LOCUS       DATUCG010000001        57085 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.001, whole genome shotgun sequence.
ACCESSION   DATUCG010000001 DATUCG010000000
VERSION     DATUCG010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 57085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57085
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.001"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            343..1119
                     /gene="rpsB"
                     /locus_tag="VCA20_000001"
     CDS             343..1119
                     /gene="rpsB"
                     /locus_tag="VCA20_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEP1833880.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKLADQAYEFIRDAAANDAVILFVGTKKQAAEAVKDEAIRAGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEINRMEEDGTFEVLPKKEVALLNKQRARLEKFLGGIADMPRIPDV
                     MFVVDPHKEQIAVKEAKKLGIPVVAMVDTNTDPDDIDVIIPANDDAIRAVKLITAKMA
                     DAIIEGNQGEDSVAAVEAELAAEPASTESIEELVEVVEGK"
     gene            1261..2301
                     /locus_tag="VCA20_000002"
     CDS             1261..2301
                     /locus_tag="VCA20_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEP1833881.1"
                     /translation="MAEITAALVKELREKSGAGVMDAKKALVETEGDIEKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVDGNVAAVVEVNAETDFVAKNAQFVELVNTTAKVIAE
                     GKPADNEAALKLAMPSGETLEEAYVNATATIGEKISFRRFVLVEKTDAQAFGAYQHNG
                     GRIGVISVVEGGDETLAKQISMHIAAMKPTVLSYTELDEQFVKDELAQINHKIEQDNE
                     SRAMVDKPALPLLKYGSKAQLTDEVIAAAEEAIKAELAAEGKPEKIWDKIIPGKMDRF
                     LLDNTQVDQAYTLLAQVYIMDDSKTVEAYLNSVNASVVEFARFEVGEGIEKASNDFEA
                     EVAATMAAALGK"
     gene            complement(2795..3421)
                     /locus_tag="VCA20_000003"
     CDS             complement(2795..3421)
                     /locus_tag="VCA20_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015445352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HEP1833882.1"
                     /translation="MLDISYQANTCHISYQGRPLGKIHSYQNPYHQTNIYLRLDLADY
                     DCTIAEQLFQSLQTSLEGKPLQVMLSSAEQEKIHFLTAAGFVCKRKCYEFEVTKQDYL
                     SQTGTSNLITARKGTAPYQTACQQVFNHYQVIHQDINPWTASQEDFEKDLPDLVYTDS
                     ENWAFIENNEIAYVCGKDEQSFGSFIQAVICQLFEQYEQISFEADDWE"
     gene            complement(3421..3759)
                     /locus_tag="VCA20_000004"
     CDS             complement(3421..3759)
                     /locus_tag="VCA20_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HEP1833883.1"
                     /translation="MNFQDYIQDFTSATVEQAQNLLAAKEEAVLFIGRATCPYCNRFA
                     PKLHKVAQDKQVTIHFLDSSQVSPELQALRDHYQVPTVPGLLVAKPTGVQVRCDSSMT
                     EEEIAAFIQD"
     gene            4028..4486
                     /locus_tag="VCA20_000005"
     CDS             4028..4486
                     /locus_tag="VCA20_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEP1833884.1"
                     /translation="MAMKNTSDYIEEHIKAILDQVSVAELRRSELASRFEVVPSQINY
                     VIKTRFTASRGYIVESKRGGGGYIRIGRITFSDKHELVHDLLNNMGEQLSATVYADIL
                     QLLFDEGLMTEREGNLFLSTGSDEILGKDASTIRARMMRQILRRLDRKEE"
     gene            4490..6940
                     /locus_tag="VCA20_000006"
     CDS             4490..6940
                     /locus_tag="VCA20_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEP1833885.1"
                     /translation="MKISTGLQRVFEDAQLVAQRYACDYLETWHVLLSFVINHDTVAG
                     AVLAEYPISISDYEHATFVVTDKVYREELDSFRILPSSKRLDETASFAKKIAEVVKAK
                     ELGTEHLFMAMLLDKRSTASQILDKVGFCFEDSDDKFRFLDLRKNLEARAGFTKEDLK
                     AIRSVMKGGKAKPTNMGQMMGMPPAPQSGGLEDYTRDLTALAREGKIEPVIGRDAEIA
                     RMIQILSRKTKNNPVLVGDAGVGKTALALGLAQRVAAGDVPVSLAKMRVLELDLMNVI
                     AGTRFRGDFEERMNNIINDIEEDGQVILFIDELHTIMGSGSGIDSTLDAANILKPALA
                     RGTLRTVGATTQTEYQKHTEKDAALVRRFAKVTIEEPTVEDSIAILTGLKGTFEKYHR
                     VRIGQAAVETAVTYAKRYLTSKNLPDSAIDLLDEASATVQNRVKGQAEETGLTSIDKA
                     LMDKKYKTVSKLLIETKEDAEASQNYDLEVTEEDVLETLSRLSGIPVAKLSQSDTKKY
                     LNLEAELHKRVIGQEEAISAVSRAIRRNQSGIRTGRRPIGSFMFLGPTGVGKTELAKA
                     LAEVLFDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYEEGGELTEKVRNKPYSVLL
                     FDEVEKAHPDIFNVLLQVLDDGVLTDSKGRKVDFSNTIIIMTSNLGATALRDDKTVGF
                     GALDLSKDHKEVEKRIFEELKKAYRPEFINRIDEKVVFHSLTETHMQDVVKVMIKPLL
                     AVTAEKGITLKLQPSALKLLAKQGYDPEMGARPLRRLLQTKLEDPLAEMLLRGDLTTG
                     STLKVGVKGEELKFDVVK"
     gene            7035..7523
                     /locus_tag="VCA20_000007"
     CDS             7035..7523
                     /locus_tag="VCA20_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HEP1833886.1"
                     /translation="MNQTIAVVFGTFAPMHKGHLDLIERAKLDCGQVCVVVSGYDKDR
                     GARIGLDLTKRFQFAQEQFKGDDLVSVCALDETDLPPYPDGWDLWLDRLLGLLDLSEH
                     QVPVFYVSEEEYAQELHSRGYQAHFSPRKFGISATLIRENPEKYRDYIALAFVDFLAE
                     QE"
     gene            7725..8108
                     /locus_tag="VCA20_000008"
     CDS             7725..8108
                     /locus_tag="VCA20_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1833887.1"
                     /translation="MRKNLLKKTVVCFINLLVVLMAVAPPAVSAQAASRIETPVETKS
                     KHFSNLEGGGDLSYINLSGYSNRFGTAASGRSGTYFGPRGTYVTRDWSVNSGGGSHGG
                     SYWKLFNRNGQRIGTYDKYGNYLRP"
     gene            8128..8361
                     /locus_tag="VCA20_000009"
     CDS             8128..8361
                     /locus_tag="VCA20_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1833888.1"
                     /translation="MQNIQLNNFEIKISERLHSIYDGKNKILVIFPDHTAICCQVKLS
                     QAGGLLVDTFWGVEYEISGNMVTIYYTDEGELE"
     gene            8358..8600
                     /locus_tag="VCA20_000010"
     CDS             8358..8600
                     /locus_tag="VCA20_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1833889.1"
                     /translation="MKQYLFRQYTIHVHPSLNVFIGKDEAEMVLYSKEPDFKLLALLR
                     WLPDKNSIRIINRWKLTYEYDGNNNIYIHYDSDYRY"
     gene            complement(8927..9931)
                     /gene="dusB"
                     /locus_tag="VCA20_000011"
     CDS             complement(8927..9931)
                     /gene="dusB"
                     /locus_tag="VCA20_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEP1833890.1"
                     /translation="MANLNTPFMIGDVEIPNRCVLAPMAGVTNSAFRTIAKEMGAGLV
                     VMEMISEKGLLYNNEKTLHMLHIDDNEYPMSIQLFGGDAEGLKRAADFIQKNTKANIV
                     DINMGCPVNKVVKNEAGAKWLKDPDKIYHIIKEVTSVLDIPLTVKMRTGWNNTDLAVE
                     NALAAESAGVAALAMHGRTREQMYTGTVDLETLTKVAGSLTKIPFIANGDIRNVEDAR
                     QRIEEVGADAVMVGRTAMGNPYIFNQINHYLETGEILPDLSFDDKLNVAFDHLTRLTN
                     LKGESVAVREFRGLAPHYLRGSAGAAKIRGAVAKAETIEQVQELFDQAREAYQERIAK
                     "
     gene            complement(9924..10790)
                     /gene="hslO"
                     /locus_tag="VCA20_000012"
     CDS             complement(9924..10790)
                     /gene="hslO"
                     /locus_tag="VCA20_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEP1833891.1"
                     /translation="MDKIIKTLSKSGHFRAFVLDSTETVKTAQEKHDTMASSTVALGR
                     TLIANQILAANEKGDTKITLKILASGAVGAIISVANTKGQVKGYIQNPDLDYKRTATG
                     EVIVGPLVGNGQFLVITDYGTGHPYNSMTPLISGEIGEDFAYFLTDSQQTPSAVGLNV
                     LLDEEDKVKVAGGFLLQVLPGATEAEIARFEKRIQEMPAISSLLASENHIEALLSAIY
                     GDDDFKRLSEEEIGFVCDCSKDRFLDALASLPKADLQEMKDEDKGVDITCQFCQTHYH
                     FDENDLEELING"
     gene            complement(10870..13989)
                     /locus_tag="VCA20_000013"
     CDS             complement(10870..13989)
                     /locus_tag="VCA20_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEP1833892.1"
                     /translation="MKIRNRSLFYTVGSVAVTAGLLLSLATSPIPSVHATEVAIENYP
                     SLAITKSEVTIQGYLIAPLNSSGTAFDATNKTNLALGASMDTAAADTIPIQLKEPLRS
                     QFNLIDHPELVGKLVRITGTSDTYMKRAGIKPATAIEIVDSSSTNVQPPTSENTATKP
                     SDLVSTPIATVRSGAQGTEYTVSGKIISLVNGWGGNGFYLQGSDGAGIYIYPGAALGY
                     QPGDTVQLTGTLGEYKGELQLTTVSNHKAISENFNTPITETNIAQLATQAQATLVSLK
                     NLTVGDIQSDSYQNSTFTVTDSEGQTVDVRLDSRTGIKTADLLNRINKGDKINLTAIL
                     STYNRKIQLKPFDLSHFEVIEKATTEAGLGKTEAVTVGRIQGASHQSPLVNQSVMLKN
                     VVVTYVTSANNFYVQDVTPDGDVKTSDGINIFTDKLKANVKVGDLVTIAGRVEEYQGR
                     GYAERDKTDLTITQIRATEVTLNGTAPVPSPIVLGLDRTIPADIIDNDGLAQFDPEQD
                     ALDFWESVEGMVVAVDDAKILGPLKNKEIYVTPATSQLPLNNVGGVNLRPEGNNTNII
                     PLLLKNGKQIVKSGDYFTGRIAGPVTYSYTNYKVYVDDSTLPTLHEGATKPETTTIIP
                     NDDKLTIASYNIENFSADSKSTSDAKVQRIAKSFVSDLHSPDIIGLIEVQDNNGATND
                     GTTDASKSAERLIAAIQAAGGPTYTYVDIAPENNKDGGQEGGNIRVGFLYNSKRVSLS
                     DKPIGTATQAVAWENGELNLSLGRIDPTNPAWAAVRKTLAAEFVFNGEKVVVLANHLN
                     SKRGDNGLYGKIQPVSFKSEEKRHILAQIIADFTKAGLAQNPNANIVMLGDFNDYEFT
                     KTIEILETGGMANLVSRHDASDRFSYFYNGNNQSLDNMLVSTNLLERYAFDMVHVNSA
                     FMEEHGRASDHDPLLVQLDVTKAQEPTQPEPSDKQTDDSGTVNNSDDNGTTNNNKPTN
                     LSTSNQTAVNADDRSGATDKGQTTVTPTAKNSQKKILPKTGEETSFVLITIGLVILSA
                     CLVKKQKES"
     gene            complement(14548..16779)
                     /gene="gshAB"
                     /locus_tag="VCA20_000014"
     CDS             complement(14548..16779)
                     /gene="gshAB"
                     /locus_tag="VCA20_000014"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HEP1833893.1"
                     /translation="MLQKLSPNSPILQATFGLERESLRINADNRVAQTPHPKKLGSRS
                     FHPYIQTDYSEPQLELITPVAHSTKEARRFLGAITDVAVRSMNKSEYLWPLSMPPVIS
                     EDEIQIAQLESDYEYQYRVGLADRYGKLLQSMSGIHYNFELGKDLTQQLFELSEETDF
                     IAFKNTLYLKLAQNFLNYRWLLTYLYGASSLAEKGFLTTEVGCVRSIRNSKYGYVNSD
                     DIHISFSSLEQYVADIEQAVQSGQLSAEKEFYSSVRLRGAKTSRDYLSKGISYLEFRS
                     FDLNPYDPLAISQETLDTTHLFALALLWLDDSEQVDEELAQAAELNNRIALSHPHTPL
                     PAEADASPILTAMTEIVAHFGLDDYYSQLIAQVEAALQDPRLTLSGKIAEQVEDGSLE
                     KFGQQQGQVFHDYAWTAPYALKGYENMELSTQMILFDAIQLGLNVEILDEEDQFLKLW
                     HGDHVEYIKNGNMTSKDNYVIPLAMANKVVTKKILDQAGFPVPAGAEFANKTDALRYY
                     GQVASSAIVVKPKSTNFGLGISIFQEPASLADYEKALDIAFSEDSHVLVEEFVAGTEY
                     RFFILDGKCEAVLLRVAANVVGDGSSTIRELVEQKNQDPLRGRDHRSPLEIINLGDIE
                     LLMLEQQGYTPDTVLPEGVQAFLRGNSNISTGGDSIDMTDQMDESYKQLAAAMATAMG
                     AWACGVDLIIPDRTKPASKEDPNCTCIELNFNPAMYLHTYTYAGPGQSITPKILKKLF
                     PEL"
     gene            17143..18435
                     /locus_tag="VCA20_000015"
     CDS             17143..18435
                     /locus_tag="VCA20_000015"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEP1833894.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIV
                     IDGTKYKLHLIPSGIFFPEKISVIGNGVVVNPKSLVKEINYLHDSGVTTDNLRISDRA
                     HVILPYHIKLDQLQEESKGENKIGTTNKGIGPAYMDKAARVGIRIADLLDKEIFAERL
                     RTNLAEKNRLFEKMYESIPIEFDEIFEEYYAYGQEIKKYVTDTSVILNDALDQGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKIDKVVGVCKAYTSRVGDGP
                     FPTELHDEIGDRIREIGKEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLETLKICVAYDLDGERIDHYPASLEQLKRCKPIYEEMPGWSEDITGVRSLDELPEA
                     ARNYVRRISELVGVRISTFSVGPGREQTNILESVWSAK"
     gene            18545..18868
                     /gene="yajC"
                     /locus_tag="VCA20_000016"
     CDS             18545..18868
                     /gene="yajC"
                     /locus_tag="VCA20_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEP1833895.1"
                     /translation="MEGLFLPLVMVAMIGFMFYSQRKQQKQRQEALNQIKKGDEIVTI
                     GGLFGIVDEIDDKKVVLDVDGVYLTFERGAIRNRVASSGTTSAVVEEAAVETTEATPE
                     SAIEE"
     gene            18982..19737
                     /locus_tag="VCA20_000017"
     CDS             18982..19737
                     /locus_tag="VCA20_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEP1833896.1"
                     /translation="MFKFNFKKKESIETAIEVPKHIAVIMDGNGRWAKKRMQPRIMGH
                     KAGMDALQKVTKTASDLGVKVLTVYAFSTENWSRPEKEVKFIMNLPVEFYDKYVPELH
                     ANNVKIQMIGDHSKLPVPTLNALYKAEQKTKSNTGLILNFALNYGGRDEVTRAVKAIA
                     QDVLDAKFDPGDIDEKLIADYLYTGTLSPTLRDPDLVIRTSGELRLSNFLPWQTAYSE
                     LYFTDIAWPDFDGEALKLAIKEYNRRHRRFGGV"
     gene            19747..20553
                     /locus_tag="VCA20_000018"
     CDS             19747..20553
                     /locus_tag="VCA20_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEP1833897.1"
                     /translation="MTNDLQKRVIFGGIALAIFIPFLLAGGMAFQFFVGLLAIIATAE
                     LIKMHRLAPNSIEGVLAMLASLVLALPLQNYLTFLPTDGNYTAYAIVVFLLLGSTVFN
                     IDHYNYSDVVFPIASSFYVGIGFHSLILARMDGLNKVFFALCLVWATDIGAYLIGRQI
                     GRRKLLPKVSPNKTVEGFVGGILSAVVVAIIFLIVDKSLLAGYSFGMMMLLVVIFSIF
                     SQFGDLVESAIKRHFGVKDSGKIIPGHGGILDRFDGMIFVFPIMHFFGLF"
     gene            20563..21822
                     /gene="rseP"
                     /locus_tag="VCA20_000019"
     CDS             20563..21822
                     /gene="rseP"
                     /locus_tag="VCA20_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEP1833898.1"
                     /translation="MKGILAFIFIFGVIVVVHEFGHFYFAKKAGILVREFAIGMGPKI
                     FAHTGKDGTLYTIRILPLGGYVRMAGWGEDKTEIKTGSPASLSLNEAGVVTRINLSGK
                     QLDSLSLPMNVTSFDFEEKLEITGLVLEESKTYKVDHDATIVEEDGTEVRIAPLDVQY
                     QNATVWGRLMTNFAGPMNNFILGILVFIILFFMQGGVANPSSNAVSITEGGALQEAGV
                     VTGDKILSVNGNTTDSYTEVATIISKAATDATTAPSFDLVVEHDGKNRHVSVTAEQVD
                     GAYRIGISPILKTGFVDKIVGGFQEAGATALRVVTALKNLIANFDVKQLGGPVAIYKV
                     SSQAAEFGLVSVLGLMAALSINLGIFNLIPIPALDGGKIVMNILEAIRRKPLKPETES
                     YITLAGVAVMVVLMIVVTWNDIIRVFF"
     gene            22155..24017
                     /locus_tag="VCA20_000020"
     CDS             22155..24017
                     /locus_tag="VCA20_000020"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEP1833899.1"
                     /translation="MKQSKMIIPTLREMPSDASVISHALMLRAGYVRQISAGIYSYLP
                     LANRVIEKAKNIMREEFDKIDAIEFLAPALLSADIWRESGRYETYGDDLYKLKNREGS
                     DFILGPTHEETVTLLARDAVQSYKQLPLNIYQIQPKYRDEKRPRNGLLRGREFIMKDG
                     YSFHASYESLDQTYDDYKAAYEAIFTRAGLEFKAIIGDGGAMGGKDSQEFMAITPDRT
                     DLNRWVVLDKSVASFEEIPEDVLEAIKTELLAWSVSGEDTIAYSSESGYAANLEMATS
                     EYKPRTAVVAEETLVKVATPDAKTIDEVAAFLNVAEEQTIKTMLFMADGEPVVALLVG
                     NDQVNDVKLKNYLGADFFDVAGPADAEKIFGAGFGSLGPVGLPENIKIIADRKVQDVK
                     NAVVGANEDGFHYTGANAGRDFQVTEYVDIREVKEGEPSPDGHGVLNFARGIEIGHIF
                     KLGTRYSDSMNANILDENGRSMPIIMGCYGIGVSRLLSAVLEQHARLFVNKTPKGEYR
                     YAWGINFPKELAPFDVHLIPVNVKDEAAMELTQSIEASLVGAGYEVLTDDRNERVGVK
                     FSDSDLIGLPIRVTVGKKAADGIVEVKIKGTGDTVEVHVDQLLETLQILTKENE"
     gene            complement(24240..24950)
                     /locus_tag="VCA20_000021"
     CDS             complement(24240..24950)
                     /locus_tag="VCA20_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEP1833900.1"
                     /translation="MNLIGELLGTFILVLLGNGVVASCILSKTKAENSGWLTIVLGWG
                     IAVTIAVYISGIFGPAHLNPAVTIAMASIGSLPWAQVPGFLLAQFAGAMLASIVLYLH
                     FYPHWQETKDSGTILATFSTGPALRHTASNLLGEALGTAVLVMGILAIGPNNVSAGLG
                     PIIVGLIVFAIGFSLGSTTGYAVNPARDLGPRIMHAILPIPNKGDSDWSYSWIPVAGP
                     ILGGVAGAILYNLLLNML"
     gene            complement(24960..25334)
                     /locus_tag="VCA20_000022"
     CDS             complement(24960..25334)
                     /locus_tag="VCA20_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002960428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HEP1833901.1"
                     /translation="MTKVGILLVSHSKNLAQGIIDLVSEVAKDIPITYCGGLEDGSIG
                     TSFEIVQERVEANPADTLLAFFDLGSARMNMELAADFTDKQILIQTVPVVEGCYTAAA
                     LLQAGADLETILEQLKELTINK"
     gene            complement(25331..25906)
                     /gene="dhaL"
                     /locus_tag="VCA20_000023"
     CDS             complement(25331..25906)
                     /gene="dhaL"
                     /locus_tag="VCA20_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HEP1833902.1"
                     /translation="MDAKQALLWMETFNKKIQENKDYLSELDSPIGDGDHGGNMARGM
                     AAVMQELSEKDYENADQVFKVVAMQLLSKVGGASGPLYGSAFMGITKASQAGADLADT
                     LQAGLDMIQKRGKAELGEKTMVDVWIPVIAALRESQLTTEIIHKAVQGTKDIAATKGR
                     ASYVGERSIGHIDPGSFSSGLLFEAMLEAGL"
     gene            complement(25917..26906)
                     /gene="dhaK"
                     /locus_tag="VCA20_000024"
     CDS             complement(25917..26906)
                     /gene="dhaK"
                     /locus_tag="VCA20_000024"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEP1833903.1"
                     /translation="MKKIINQPEHVVDEMLKGLVFMHQDLVNRINGFDVIIRKAEKTG
                     KVGIISGGGSGHEPSHAGFVGKGMLSAAVCGAVFTSPTPDQILEAIKAADEGAGVFMV
                     IKNYSGDIMNFEMAQELAEMEGIEVASVIVDDDIAVENSLYTQGRRGVAGTILVHKIL
                     GHAAETGKSLAEIKELADRLVPQIKTIGLALSGATVPEVGKPGFVLEEDEFEYGVGIH
                     GEPGYKKEKLQPSAKLAEELVEKLAEGFDIQDGEHYGVLINGLGATPLMEQYVFANDV
                     AQLLAKKGVVIDYKKIGNYMTSIDMAGLSLTLIKLEDPSWIDALQAPVEVVAW"
     gene            27053..27598
                     /gene="dhaS"
                     /locus_tag="VCA20_000025"
     CDS             27053..27598
                     /gene="dhaS"
                     /locus_tag="VCA20_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase transcriptional
                     activator DhaS"
                     /protein_id="HEP1833904.1"
                     /translation="MPTSLITKKRIAKAFKKQLSQKSFDKISVVDIMQEAGIRRQTFY
                     NHFLDKYELLDWIFETELQEQVTDNLTYISGIKLLDELLYYIESNQSFYRQLFEIKGQ
                     NDFVSYLEGYFMVLMEKISNEYQLQEGKMISQQDKHFLILYHANAWIGLIKEGLTQSP
                     CQIHSYGKQMVALVRESFLIL"
     gene            27609..28598
                     /gene="dhaQ"
                     /locus_tag="VCA20_000026"
     CDS             27609..28598
                     /gene="dhaQ"
                     /locus_tag="VCA20_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DhaKLM operon coactivator DhaQ"
                     /protein_id="HEP1833905.1"
                     /translation="MVFIRNRQATIIEDYLDGFCRTNPKVKKVKGLPIVLQKVQDDHL
                     VPIISGGGSGHEPAHIGFVGKGMLTAAVYGEIFTPPTAEEVLAAIRAVDKGKGVFLIV
                     KNFEADLVSFRKAIELARQEGIAVKYIVSHDDISVDTKNNFRMRHRGLAGTILLHKIL
                     GAAALTGYSLDELEQLGLSLATEIATIGFATKSAYLPNAALPLFDLEEGQISYGIGIH
                     GEEGYRTVRFESSEQLANEIVNKLKLKFRWKEGEDYILLVNNLGSMSEVEQGIFLNDI
                     CQFLELEGLQLSYIKTGKFMTSLDMGGISVTLCRVKDRKWLDFLEAPTEAAAW"
     gene            28715..33106
                     /locus_tag="VCA20_000027"
     CDS             28715..33106
                     /locus_tag="VCA20_000027"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002907973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEP1833906.1"
                     /translation="MSDKFQLLLQQIGIPLDARQSGAFSTATIEKVVLHKVSKLWEFT
                     FRFETPLPLMDYQLFKARLATEFEKVGNKIQFSIVSDAEAFEAGLVEAYYPEAFTEDL
                     CQSAGFKALFQPLEVTYRDGVLWIKGPETIDTDHFRKNHLPNLVEQYKRFGFGNLAVD
                     IQVCQEMTQQQAEIFHAQNEEIYQQANEENLAALEQLAQMVPPPEAAQPFVPEYKKNR
                     PAKVNIEKAEITPMIEVDSEENRIVFEGLVFEVEQKTTKTGRVIINFKMTDYTSSFTL
                     QKWAKNEEEAQKFDMVKKGNWLRVRGNVETNNFTRDLTMNVQEVQEVKKEIRKDLMPE
                     GEKRVEFHAHTNMSTMDALPAVEDLVARAAAWGHKAVAITDHGNVQSFPHGYHAARKA
                     GIKPLFGMEANIVEDSVPIAYNEADVVLSDATYVVFDVETTGLSAVNNALIQIAASKM
                     HKGNIIAEFDEFIDPGHPLSQFTTDLTGITDEHVRGSKPLEQVLREFQDFCQDSVMVA
                     HNATFDVGFMNVNYERAGLPIISQPVIDTLEFARNLYPDFKRHGLGPLTKRFGVALEH
                     HHMANYDAEATGRLLFIFLKDALEKHNLTNLNQLNTELIAEDSYKKARVKHATLYVIN
                     QVGLKNMFKLVSLSNTKYFEGVPRIPRTVLNAHREGLILGTACQEGEVFDDLLSKGID
                     EAVKTAAYYDFIEVMPPALYAPMIAKEQFKDMAEIEETIKQLIEVGRRAGLPVLATGN
                     VHYIDPEEEIYREIIVRALGQGAPINWTIGNGENAQPAPLPKAHFRTTSEMLDEFAFL
                     GESLTREIVITNPNAMLDRFEDVQVVKTDLYTPYIEKAEETVAELTYQKAFEIYGNPL
                     PDIIDLRIEKELTSILGNGFAVIYLASQMLVHRSNERGYLVGSRGSVGSSFVATMIGI
                     TEVNPMPPHYVCPNCQHSEFITDGSYGSGFDLPDKDCEKCGTKYKKDGQDIPFETFLG
                     FDGDKVPDIDLNFSGDDQPSAHLDVRDIFGEENAFRAGTVGTVAAKTAYGFVRGYERD
                     YGKFYRDVEVERLAAGAAGVKRTTGQHPGGIIVFPDYMDVYDFTPVQYPADDVTASWQ
                     TTHFNFHDIDENVLKLDILGHDDPTMVRKLQDLSGIDPQTIPADDKGVMALFSGTEIL
                     GVTPEQIGTPTGMLGIPEFGTNFVRGMVEETKPTTFSELLQLSGLSHGTDVWLGNAQD
                     LIKAGIANLSTVIGCRDDIMVYLMHAGLPPKMAFNIMERVRKGLWLKISEEERNSYIQ
                     AMKDNKVPDWYIESCGKIKYMFPKAHAAAYVMMALRVAYFKVHHPLYYYCAYFSIRAK
                     AFDLATMSGGLERVKAKMEEIALKKKNNEASNVEQDLYTTLELVNEMLERGFKFGKLD
                     LYKSHATDFLIEDDTLIPPFVAMDGLGENVAKQVVAARAEGEFLSKTELRKRGGLSGT
                     LVEKMDEMGILGKMPEDNQLSLFDDLF"
     gene            33250..33852
                     /locus_tag="VCA20_000028"
     CDS             33250..33852
                     /locus_tag="VCA20_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012997947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEP1833907.1"
                     /translation="MSNFAKLQETRRTIYALGKDLPVSNEEVVALVEKAMKEAPSAFN
                     SQSSRAVVLFGAESEAFWNEIAYSELEKVTPAEAFEGTKGRLASFAAGAGTILFFEDQ
                     DVVKGLQESFPLYAENFPIWSEQAHGINLYAVWLAFAEKNIGMNVQHYNPLVDAQVAE
                     KYGIPANWKLRAQAPFGSIAAPAADKDYMADSDRVKVFGN"
     gene            34550..35791
                     /locus_tag="VCA20_000029"
     CDS             34550..35791
                     /locus_tag="VCA20_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEP1833908.1"
                     /translation="MVLPNFKENLAKYAKLLVSTGINVQPGHTVQLTIGVEQAELARL
                     IVKEAYARGAKEVLVNWLDDVIARERLVNVDVEFLEQVHPQRITEMNYLLERKASRLV
                     VLSEDPGAYDGVDPEKLSRNARALSQALNPMRQASQANKISWTLGAASGLEWAKKVFP
                     NAASDEEAVDLLWDQIFKTCRIYEEDPIKAWEEHEARLVAKAKVLNDEQFVKLHYTAP
                     GTDLVLGMPKNHLWEAAGSVNAQGEHFIANMPTEEVFTAPDYRVADGYVTSTKPLSYN
                     GNIIEGIKVTFKDGEIVDVTAEKGDEVMKKLVFDNAGARGLGEVALVPDKSPISQSGV
                     TFFNTLFDENASNHLAIGQAYAFSIEGGTEMSQEELKEAGLNRSDVHVDFMIGSNKMN
                     IDGIREDGTRVPIFRDGEWAI"
     gene            35851..36084
                     /locus_tag="VCA20_000030"
     CDS             35851..36084
                     /locus_tag="VCA20_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEP1833909.1"
                     /translation="MLTSIIAGAIIGLIAGALTSSDDRRGCLGNIILGLAGSWIGQLL
                     FGDWGPQFAGMALVPSVFGAVLLVAIFSSSRRN"
     gene            36465..38027
                     /locus_tag="VCA20_000031"
     CDS             36465..38027
                     /locus_tag="VCA20_000031"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEP1833910.1"
                     /translation="MRVIEFLDTTLRDGEQTPGVNFSIKEKVTIAKQLEKWGISAIEA
                     GFPAASPDSFEAVRQIAAAMTKTAVTGLARSVKSDIDACYEALKDAKYPQIHVFIATS
                     PIHREFKLQKTKEEILAQITEHVSYARERFEVVEFSPEDATRTELDYLLEVVQTAVDA
                     GATYINIPDTVGFTTPQHYGEIFRYLTTNVKSDREIIFSPHCHNDLGMAVANTLSAIK
                     NGAGRVEGTINGIGERAGNAALEEVAVALEIRKDFYDVTSPIVLKETLNTSELVSRFS
                     GIAIPRNKAVVGGNAFSHESGIHQDGVLKNPLTYEIITPELVGVKKNSLPLGKLSGRH
                     AFVEKLKELDLYFEEGDVASLFVRFKNLADKKEKITDADIRALVAGTEISNRDGFQFK
                     DLRLDSQSDGSIQAQVIFINQDEEEVVVAESGKGSVEAVFNAIDQFFHQEISLERYHI
                     DAITDGIDAQARVLVAVENKATETVFNASGIDFDAVKASAIAYIHANVMVQKENSGQI
                     DKKLSEKELPHI"
     gene            38036..39088
                     /gene="leuB"
                     /locus_tag="VCA20_000032"
     CDS             38036..39088
                     /gene="leuB"
                     /locus_tag="VCA20_000032"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEP1833911.1"
                     /translation="MTKKIVALAGDGIGPEIMEAGLAVLEAVAGQVGFDYEIEERAFG
                     GAGIDAAGHPLPDATLQACRQADAILLAAIGSPRYDDAAVRPEQGLLQLRKELGLFAN
                     IRPVKIFDSLKDYSPLKADRLDGVDLVMVRELTGGIYFGEHILETDRASDSNTYQAKE
                     IERVVRSAFDLAQKRRKKVTSIDKQNVLATSKLWRKVADEVAKDYPDVTLEHQLVDSA
                     AMLLITNPSRFDVLVTENLFGDILSDEASVLTGTLGVLPSASHAVAGPSLYEPIHGSA
                     PDIAGQGIANPVSMILSVAMMLEESFDLVQAGQTIRQAVEAVFAEGIFTKDLGGSAST
                     KEMTAAIIEQLERRSP"
     gene            39085..40476
                     /gene="leuC"
                     /locus_tag="VCA20_000033"
     CDS             39085..40476
                     /gene="leuC"
                     /locus_tag="VCA20_000033"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEP1833912.1"
                     /translation="MSGKSIFDKLWDCHVITGQEGQPQLLYVDQHYVHEVTSPQAFQG
                     LRDTGRPVRRPDLTFATFDHNVPTVDIHNIRDVISKAQMDALAKNIEDFGIPHAAHGS
                     EHQGIVHMVGPETGRTQPGKFIVCGDSHTATHGAFGAIAFGIGTSEVEHVLATQTIWQ
                     VKPKRLLVEFVGQAQRGVYAKDFILALIARYGVDCGLGHVVEFAGPVIDRLSMEERMT
                     ICNMSIEFGSKMGIMAPDQTTFDYLRGRECAPADFDAAVADWKELYSDADTVYDKHLI
                     LDVSELAPMVTWGTTPAMGVSFDETFPVIRDHNDERAYAYMDLAPGQKAADIPIGYVF
                     LGSCTNARLSDLQLAARIVKGRKIAEQVTAIVVPGSRPVKRAAEKLGLDKIFMEAGFE
                     WRDPGCSMCLGMNPDKVPAGVHCASTSNRNFEDRQGFGAKTHLCSPAMAAAAALYGRF
                     VDTRQLDILKEGV"
     gene            40476..41069
                     /gene="leuD"
                     /locus_tag="VCA20_000034"
     CDS             40476..41069
                     /gene="leuD"
                     /locus_tag="VCA20_000034"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEP1833913.1"
                     /translation="MHQFTTWTGTTVPLMNDNIDTDQLLPKQFLKLIDKKGFGKYLLY
                     AWRYLDDNYTDNPDFILNQPEYQGASILISGDNFGAGSSREHAAWALADYGFKVIIAG
                     SFGDIHYNNDLNNGILPIIQPKEVRDQLAQLGPDQEITVDLADQLIRTPFWEFPFDIE
                     QDWKHKLLNGLDDIGITLQYQDLIAEYEDNRPSYWQN"
     gene            41413..42198
                     /locus_tag="VCA20_000035"
     CDS             41413..42198
                     /locus_tag="VCA20_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002276310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HEP1833914.1"
                     /translation="MTKQIHWDGALSQEGFDIIKGEGGVIVCPTKVGYIIMTADKAGL
                     ERKFDAKERKRNKPGVVLCGSMEELRELAQLTPEIDAFYQKHWDADILLGCILPWKPE
                     AYAKLQAYGDGREELMTDVRGTSCFVIKFGVAGEQIAKEMWEKEGRMVYASSANPSGK
                     GNRGKVEGIGERIASKVDLIIEADDYVASIQPDKTIETRYEQGVMVSMVDKDGKLIPE
                     QGADSRSISPCPVVIRKGLDIDKIMMHLSDHFNSWDYRQGEYY"
     gene            complement(42263..43123)
                     /locus_tag="VCA20_000036"
     CDS             complement(42263..43123)
                     /locus_tag="VCA20_000036"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP1833915.1"
                     /translation="MRWDYGSVYKSIRKSKHLSQEQICGDYLNRTTLVRFEKNQTIPS
                     YELMRFLLKQVDMSFEEFEYLCNYYQPSQRQQLLYDIDNLRNPTTKTMEDLIKRCQDH
                     LKNEPNDIPIHRKCLLLETVVAIRKSSSITQLSDEAETLSKLLWSQLEGYDNWYHNDI
                     ILVGTLLSKISSPDTLEETANLLLKRLEKYKDYKKIQPTILSHYRSLSYFFLEQRQYS
                     KSTCFATKLMDLAKKEKRYDQLARAYVYLGIAQNKQELVEKGLQILELTDEKRLVDNL
                     LFLIKQHQTD"
     gene            43250..43462
                     /locus_tag="VCA20_000037"
     CDS             43250..43462
                     /locus_tag="VCA20_000037"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1833916.1"
                     /translation="MKQIDKNTLFLSAVIVLSCSILLFHMDPIQIVDREIWLWLNSFI
                     HVLFLKPAASLGFLMGLYLLYKALKK"
     gene            43529..44413
                     /locus_tag="VCA20_000038"
     CDS             43529..44413
                     /locus_tag="VCA20_000038"
                     /inference="COORDINATES: protein motif:HMM:NF012235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1833917.1"
                     /translation="MLKVVNVCKRYGAHQVLSYVSFELQAGDLVALVGPNGVGKSTLL
                     NIISNTETADRGSVTINGRPNSDRAIFQDMSVMLDAQALYPQLTGYDHLTYVAATHKL
                     GKREVDALVEELGMGYYVKKRVAGYSMGMKQKLLFAMAVLPKPKLLLLDEPHIGLDPT
                     NIIQQREFLLNLQAEGVAILLSSHHLSEIEKLTNQVYFLKSSKLIATEVELTADYDYH
                     LAVENSKQVQEFLRDYPQLLWKKAEQGLVEIFLPERDLLDCLDRIPIQQVAGLTKASD
                     YMESLYRDLYVEEGGEEI"
     gene            44410..46752
                     /locus_tag="VCA20_000039"
     CDS             44410..46752
                     /locus_tag="VCA20_000039"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1833918.1"
                     /translation="MRLNFEIKKILWSSLYCLLVLGFLGLAYLPISQRQAHQAAYVET
                     YKKELSELTSVLAEIKIEEGSEEAPRMLGLKSDIESVGQALDEGKFEAIPNYRKLMYQ
                     DLDYFISRNRQLFQTRNSLTQTEVERLTRWNDWLLAYQLPDGPENKAWSTGRIVQAYL
                     ESLFGFLPVFLVLGFLLANQLSEKTLAHQVWQLRQWDSLSLQGMRRLVAYLLSLLLAV
                     GIGLLFILVYDGLTGQLAVSSFQAPLEILGQSQLIGSWLYGLILLGFWLILLLAIRLL
                     FGFLDHLLASRLTFVVIGLLLALVLSYQAGAVAGWSGQILPLFAFPSFLDTAEFSQVF
                     VAVDVFLLLTIGLFFAYYVLTEKKRLVLSVGHQKEWETGPLDKVWSFEILILSRLNSW
                     KGLVLVNLLLALLFSLLAGLEKQEVAQEERVSMELVAQSYKQDGIYVESLQSEINRLR
                     QLVKEDAGYQEELELRLGLLQEYRVYKDLYVKQWQVYQEKPQALPASHLAMLEAEQVY
                     LWAREEKAFANGDIGASQTVDNLRRYQTRNQVSQYYWTQLMEAGVPATKRGKDVILTS
                     LENRFDTNDALVPVDDPSWDRSRDLSGIGSLRSLFDGPAYLLVLLLAVWIGSRGKAVE
                     VVEKNWRLYQTQPVDLRQVLFTKWLLGLGQALGFTLLFLVSLFLVNSLVGGIGYLEDG
                     LILLSHSERGIFLELLRGQELWAWFLPNAPYLLWMVFGLLVLEIVLVSLNHLLQVRFS
                     NRLVVFILLAGLLALLDYLLFQDLILDGQEIFRQLALLFS"
     gene            complement(46798..47412)
                     /locus_tag="VCA20_000040"
     CDS             complement(46798..47412)
                     /locus_tag="VCA20_000040"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEP1833919.1"
                     /translation="MSVIERLTKAAHLIDMDDIIREGHPTLRQVAEEVAFPLSDQEII
                     LGEKMMQFLKHSQDPVMAEKMKLRGGVGLAAPQIDVSKRIIAVLVPNPEDEEGNPPAQ
                     AYSLQEVMYNPKIVAHSVQEAAMEGGEGCLSVDREVQGYVVRHARVTVDYMDKKGETH
                     RIKLKGFNAIVVQHEIDHLNGVMFYDRIDPEHPFAVKEGMLVIE"
     gene            47542..48711
                     /locus_tag="VCA20_000041"
     CDS             47542..48711
                     /locus_tag="VCA20_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEP1833920.1"
                     /translation="MKRILEKASLLALSTMLVSTFAVSPAIPQMIEHFAQQGIAAPQV
                     ENLITVTSFAIMVTLLANGLVVRFLSERNIIIAGLLLMAIGGALPMFLTAYPLILLAR
                     ILLGFGIGLINARAINIIGNFFTGQERVQMMGLRGSAEVLGSAGLTLLVGWLTQFGWQ
                     PAFLVYLFALVILALFLLFVPKEELLARMEVKVEENAPKVKLDKTMWMMGIYLAFLAS
                     FVINVNTFLTIRIPQIVLAKGIGTAQQASLILSLMQVMGIVAGTVFSSLVGRLKGWLL
                     AVSYVVFGLAVVGIAFADNLWVLGLGGMVSGFFYSIILTIVFSQVTDRAPKALLNAVM
                     TIVLMGCNIGGATSAILPTYLEKLNPTPTGAFGIYAIGCALISAGLIYKQLKKWE"
     gene            48875..49084
                     /locus_tag="VCA20_000042"
                     /pseudo
     CDS             48875..49084
                     /locus_tag="VCA20_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028520.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            49091..49540
                     /locus_tag="VCA20_000043"
     CDS             49091..49540
                     /locus_tag="VCA20_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEP1833921.1"
                     /translation="MDYFYIMPLPQDLALEIANQWRYQPPLDAYTISGNPETYAEMIS
                     PEARGNRFFAVIRNAALMGYFCMDQDGETVDIRLGMKPSLTGQGNGRAFYQTIEDYLV
                     EHVQPASIKLTVASSHQVAQKFFPALGFTEIEKQAEYSKMEKKVVCD"
     gene            49531..50259
                     /locus_tag="VCA20_000044"
     CDS             49531..50259
                     /locus_tag="VCA20_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEP1833922.1"
                     /translation="MRLDKFLVDCGVGSRTEVKKLLKNKQVTVNGQVETSPKQQIDEK
                     SDQIALAGQVLSHETFVYYLLNKPKGVISATEDDRHRTVLDLLDSTARQKEVFPVGRL
                     DIDTHGLLLLTNNGQLAHAMLSPKKHVDKLYRAQVDGIMTQEDVERFAAGIELKDFTC
                     QPAQLTILSTDEAKQTSLVDITIREGKFHQVKRMVQACGKTVTDLQRLTMGPIRLDPK
                     LALGEYRRLTAAELKQLEVFGVEL"
     gene            complement(50301..50882)
                     /locus_tag="VCA20_000045"
     CDS             complement(50301..50882)
                     /locus_tag="VCA20_000045"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002959164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEP1833923.1"
                     /translation="MKALEARILKDGQVLGENILKVDSFLTHQVDFHLMKEMGQVLAD
                     AYRSKEITKVVTIEASGIAPAVYVAESLDVPMIFAKKHKNITMTEGILTAEVYSFTKQ
                     VTSTVSIASKFLSPEDRVLIVDDFLANGQAAKGLIDIIQQAGAQVAGVGIIIEKSFQD
                     GRQLLLDAGVPVTSLARIEKFQDGQVVFAAADI"
     gene            51099..52409
                     /locus_tag="VCA20_000046"
     CDS             51099..52409
                     /locus_tag="VCA20_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEP1833924.1"
                     /translation="MSQKITNEHSSDMLYGIDEQPPKGMAVLLAFQHILAAFAGIIAV
                     PLVVASALGLSVEDTSIIVSASIFVAGIATILQSKGVGPVGSRVSGMMGTDFTFANPA
                     ISVGSQLGIAGIVGATIAGSFVEIALSRFVKPLMRFFPPLITGTVVSLIGITLMPVSM
                     DWAAGGAGASDYASVENISIAFIVLVFTLALNHYGKGMLKTASVFFGMVFGYVLCILL
                     GKVDMSAVGEAAWFALPKIFHYGVKFDLSSILAFIPAYVVSLIGTVGIMMAIGEASNQ
                     KIPSERAANGVLADGVGSLIAGIFGAGPNTAFSQNVGLITLTKVASRHVMILAGIILT
                     LLGVFPKLSALISIMPQPVLGGVGIIMFGLVAAQGIKTLATVKIGDRELLIISIAFAL
                     GIGVTVRPELLSHLPSALQMVFSSGISTGALAALVLNLVLKEEK"
     gene            52976..53485
                     /locus_tag="VCA20_000047"
     CDS             52976..53485
                     /locus_tag="VCA20_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1833925.1"
                     /translation="MLKYIRALPLVIVAGAVVYFTIFICIDNGDWTYVVTRALGLKEE
                     HANNVSLIVIGAQSLFLTSDWFYKRIRAFLNRRLEKAKGLNRQIYLGYKAGIEFLMAL
                     PKRTILFTFYLLLVVAESVGLIEKAKDYAAVIIFIIAVDRVTKIWPEEKRRMIEFSQK
                     IKKRIKSVG"
     gene            53626..53844
                     /locus_tag="VCA20_000048"
     CDS             53626..53844
                     /locus_tag="VCA20_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP1833926.1"
                     /translation="MKNIRLKLARVEKDMTQGDLAEAIGVTRQTIGLIEAGKYNPSLS
                     LCLSICHCLGKSLDQLFWEEATNEKNCD"
     gene            53825..54298
                     /locus_tag="VCA20_000049"
     CDS             53825..54298
                     /locus_tag="VCA20_000049"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1833927.1"
                     /translation="MKKIVTDERVRQEENQVFAWVGRAMNFLLPLSFLIKRLVLKWPF
                     DTYVFELFAMLVVSVYLFYGYWRKGLDMEQGPAWKGYLYLEGVILGTTLLVAWTNYQT
                     YGHHYTGIWDGHFWAVVFIFFISMTCLVLLLLNIVSWVNSYRQKQVEKELEEEME"
     gene            54517..55794
                     /locus_tag="VCA20_000050"
     CDS             54517..55794
                     /locus_tag="VCA20_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEP1833928.1"
                     /translation="MSTKANLLFDVHEKPAPLQVILLSFQHVFAMFGATILVPLILGL
                     PVSVALFASGVGTLIYQVATQFKVPVYLGSSFAYISAMALAIKEMGGDVSAAQTGILF
                     VGLIYVLIAALVKVIGTKWIDTLLPPIVIGPMIIVIGLGLANSAVTSAGFVADGDWRN
                     VVAAIATFLIAAFVNTKGKGFAKIVPFLIAIIGGYVIAIFLGLVDFTPVLEAAWFELP
                     GFYLPFETGTFKSYNFYFGPEMIAILPIAVVTVAEHIGDHTVLSQICGRQFLKDPGLS
                     RTLIGDGVATAVSAFIGGPANTTYGENTGVIGMTRIASVSVIRNAALIAIAFSFLGKF
                     TALISTIPSAVLGGMSILLYGVIASNGLKVLIESRVDFGQVRNLIIASSMLVLGLGGA
                     VLNIGAITLSGTALSAIVGIILNLILPKAEKAE"
     gene            complement(55897..57005)
                     /locus_tag="VCA20_000051"
                     /pseudo
     CDS             complement(55897..57005)
                     /locus_tag="VCA20_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612110.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT        15230 a        11451 c        13911 g        16493 t
ORIGIN      
        1 atgggctcac atcaaaaaac acctcagaag agtattgcca aattgcgata cgtttcttga
       61 ggcactttag tcctgctctt gtttcagttg actataaaat ggatgaagtt aaaaatcata
      121 aaaaagtagt aacataaaga gagacatatc ccaatctatg agcttggcat aatatagaga
      181 tttgtgtact gaaatgcttg attttcacct aaaactttgg tatacttact aagttgtcta
      241 aacaacaaat actcatctcg gatcaattgt ggtcttgttg ccgaaaggtt aggctgcaag
      301 tcgaagttcg ggagaggaga aaaaacaaaa ggagaaatac tcatggcagt aatttcaatg
      361 aaacaacttc ttgaggctgg tgtacacttc ggtcaccaaa ctcgtcgctg gaatcctaag
      421 atggctaagt acatcttcac agagcgtaac ggtatccacg ttatcgactt gcaacaaact
      481 gtaaaattgg ctgaccaagc ttacgaattc atccgtgacg ctgcagcaaa cgacgcagtt
      541 atcttgttcg taggtactaa aaaacaagct gctgaagctg ttaaagacga agctatccgc
      601 gctggtcaat acttcatcaa ccaccgttgg ttgggtggaa ctcttacaaa ctggggtaca
      661 atccaaaaac gtatcgctcg tttgaaagaa atcaaccgta tggaagaaga tggaactttc
      721 gaagtgcttc ctaagaaaga agtagcattg ttgaacaaac aacgtgctcg tcttgaaaaa
      781 ttcttgggcg gtatcgctga catgccacgc attccagatg taatgttcgt tgttgaccca
      841 cataaagagc aaatcgctgt taaagaagct aaaaaattgg gtatcccagt tgtagcgatg
      901 gttgacacaa acactgatcc agatgatatt gatgttatca tcccagctaa cgatgacgct
      961 atccgcgctg ttaaattgat cacagctaaa atggctgacg ctatcatcga aggtaaccaa
     1021 ggtgaagaca gcgtagcagc agttgaagct gaattggcag ctgagccagc atctacagaa
     1081 tcaatcgaag aattggttga agttgtagaa ggaaaataag taatgaaggc gaattaaaat
     1141 caatacggtt ttagctcgcg tataattcta catcattatt taacaacaaa aaaaacaatc
     1201 ctagaggggc agggctgagc ccgctcctct attttataaa tacaaacaag gagaatagac
     1261 atggcagaaa ttacagcagc tctcgttaaa gaattgcgtg aaaaatcagg tgctggcgtt
     1321 atggacgcga aaaaagcatt ggttgaaact gaaggtgata tcgaaaaagc gattgaattg
     1381 cttcgtgaaa aaggtatggc taaggcagct aagaaagctg accgtgtagc agctgaaggt
     1441 ttgacaggtg tttacgttga tggtaacgta gcagcagttg ttgaagtgaa cgctgagaca
     1501 gacttcgttg cgaaaaacgc tcaattcgtt gagttggtaa acactactgc taaagtaatt
     1561 gcagaaggca aaccagctga caacgaagca gctcttaaat tggctatgcc ttcaggtgaa
     1621 acccttgaag aagcatacgt aaacgcaact gcaacaatcg gtgagaaaat ctcattccgt
     1681 cgctttgttt tggttgaaaa aacagatgct caagcatttg gtgcatacca acacaacggc
     1741 ggccgtatcg gtgttatctc agttgttgaa ggtggcgatg aaactcttgc aaaacaaatc
     1801 tcaatgcaca tcgctgcgat gaaaccaact gttctttctt atactgaatt ggatgagcaa
     1861 ttcgttaaag atgagttggc acaaatcaac cacaaaatcg aacaagacaa cgaaagccgt
     1921 gcaatggttg acaaaccagc attgccattg ttgaaatacg gttcaaaagc tcaattgact
     1981 gacgaagtga ttgcagcagc tgaagaagct atcaaagcag aattggcagc agaaggcaaa
     2041 ccagaaaaaa tctgggacaa aatcatccca ggtaaaatgg atcgcttctt gcttgacaac
     2101 actcaagttg accaagctta cacacttctt gcacaagtgt acatcatgga tgacagcaaa
     2161 acagttgaag cttacttgaa ctcagtgaac gcttcagttg tagaatttgc tcgtttcgaa
     2221 gtgggtgaag gtatcgagaa agcttcaaac gactttgaag cagaagttgc agcgacaatg
     2281 gcagcagctc ttggtaaata attgctagtc ttcattttat cttttattac ttcgttaact
     2341 cgccttgccg tacttcagta cagcctgcgg ctcgtttcct agtactaaaa gcaaactgat
     2401 agactattca aaatcacaag aacttccgaa tgggagttct tttttggaaa aatcggtctt
     2461 agggcggatg tctgaaaaga caatatcgga tatactgata tcaatcctaa atatttttca
     2521 taatctcctt aaaaagcacc ggccttgtgg tcggtgcttt ttgttagatt tctaaaatat
     2581 aagtgtccca gctaggcttg tttggttgtt ggaattggtc agctaaacgc atggcggctg
     2641 ggctcaagtc cagccacgct tctcagagtt cgtgtcaaca tctcagcgca gtggttgatt
     2701 ggcagatttg ttcgtgtatt acactctaaa tctgacctaa tcaactgtgc gggggtgaga
     2761 agacgaactc tttttagacc ggtcgagttc tttctcactc ccagtcatca gcctcaaagc
     2821 taatttgttc atattgttca aatagttggc aaataactgc ttggatgaag gaaccaaagc
     2881 tttgttcgtc ttttccgcat acgtaggcaa tttcattgtt ttctatgaag gcccaatttt
     2941 ctgagtctgt atatactagg tcaggtagat ccttttcgaa gtcttcttga ctggcagtcc
     3001 aaggattgat gtcttggtgg ataacttgat agtgattaaa gacttgctgg caagctgttt
     3061 ggtaaggtgc ggtgcctttg cgagctgtga tgaggttgga agtgcctgtt tgactgagat
     3121 agtcttgctt ggtcacttca aactcgtaac attttcgttt gcagacaaag cccgctgcgg
     3181 tgaggaagtg aattttttcc tgctctgcag aggagagcat aacctgcagg ggcttacctt
     3241 ctaaggaagt ttgtagggac tggaagagct gctctgctat ggtgcaatcg taatcggcca
     3301 aatctagtcg cagatagata ttggtctggt ggtagggatt ttggtagctg tgaatcttgc
     3361 cgagtggtct tccttgataa gagatgtggc aggtgttggc ttggtaggaa atgtctagca
     3421 ttagtcttga ataaaagctg caatctcttc ttcggtcatg ctggaatcgc agcggacttg
     3481 gactccagta ggtttagcga ccaggagacc tgggacggta ggaacttggt agtggtcgcg
     3541 gagggcctgg agttcagggc tgacttggct ggagtcgagg aagtggatgg tcacttgctt
     3601 gtcttgggct accttgtgga gttttggtgc aaaacggttg cagtaaggac aggttgctcg
     3661 tccgatgaag agaacagcct cctctttggc agctagaagg ttctgtgcct gctccacagt
     3721 cgcacttgta aagtcttgaa tgtaatcttg aaagttcatg gtataagata taaggggcct
     3781 taagcggaca cggaaaaaat aggaaacaga acgacgacta gttctcgttt atctttttgg
     3841 ctagtccgta gcccgtattc agttcaacta aatacggaag ccctagtcct ttctgttttt
     3901 tcttttatta taggataaag cagagcagat ttcaaaattc tgctacgcta gttgctaggc
     3961 ttttgctttc aaaattcgtc attttaaggt ataatggtca aagatagtca aagaaagagg
     4021 cgatactatg gcgatgaaga atacatcgga ttatatagaa gagcatatta aagccatctt
     4081 ggaccaagtc agtgtagctg agttgcgtcg aagtgagttg gccagtcggt ttgaagtggt
     4141 gccaagtcag attaactacg tcattaaaac ccgttttaca gctagccgtg gctatattgt
     4201 tgagagtaag cgtggtggtg gtggttatat ccgaattggg cggattacct tttcggataa
     4261 gcacgaactg gtccatgatt tattaaacaa tatgggtgag cagttgtctg cgacggttta
     4321 tgcggatatt ttacagctcc tttttgatga aggattgatg acagaacgag aaggcaatct
     4381 ctttttgtcc acaggatctg atgaaatatt aggaaaagat gcaagtacta ttcgagcacg
     4441 catgatgcgt caaatactac gacgtttaga taggaaagag gaataattca tgaaaatttc
     4501 aactggttta cagcgggttt ttgaagatgc ccagttagtg gcccaacgct atgcctgtga
     4561 ttacttggaa acctggcatg ttctcttgtc tttcgttatc aaccatgata cagtcgccgg
     4621 cgctgttttg gcagaatatc ctatttcaat ttctgactat gaacatgcaa cttttgttgt
     4681 gaccgacaag gtctatcgtg aagagttgga tagttttcgt attttaccat cttctaaacg
     4741 tttggatgaa acggctagtt ttgccaagaa aattgcagaa gtcgtcaagg ctaaggagct
     4801 gggaacggaa catttgttta tggctatgtt gttggacaag cggtcaacag ctagtcagat
     4861 tttggacaag gtaggttttt gttttgaaga ctcagatgat aagtttcgtt tcttggattt
     4921 gcgtaaaaac ttggaggcac gtgcgggatt taccaaagag gaccttaaag ctatccgcag
     4981 tgtgatgaag ggtggcaaag ccaaacctac caatatggga cagatgatgg gaatgcctcc
     5041 agcacctcaa agtggtggat tagaagacta cactcgtgat ttgacagctt tggcgcgtga
     5101 agggaagatt gaacctgtta tcggtcgaga cgctgagatt gcacggatga tccaaattct
     5161 atcacgtaaa accaagaata acccagttct agttggtgat gctggtgtcg gaaaaactgc
     5221 gcttgctttg ggtcttgctc aacgtgttgc tgcaggtgat gtccctgtca gcttggctaa
     5281 gatgcgggtt ttggaacttg acttgatgaa cgttatcgct ggaactcgtt tccgtggtga
     5341 tttcgaagag cggatgaata atatcatcaa cgatattgag gaagatggtc aagtcatcct
     5401 tttcatcgat gagttgcata ccattatggg ttctggctcg gggattgatt cgaccttgga
     5461 tgcagccaat atcctcaaac cagctcttgc tcgtggcacc ctccgtacgg tcggagcgac
     5521 gactcagaca gagtaccaaa aacatactga gaaagacgca gccctggttc gtcgttttgc
     5581 caaagtgacc attgaagagc cgacagtgga agatagcatt gccattttga ctggtttaaa
     5641 aggtactttt gaaaaatacc acagggtccg tattggacaa gcagctgtag agactgctgt
     5701 gacctacgct aagcggtatt tgaccagtaa aaacctaccg gattcagcca ttgatctgct
     5761 ggatgaagcc agtgcaacgg tacaaaatcg agtgaaaggg caggctgagg aaacaggact
     5821 gacgagcata gacaaggcct tgatggataa aaaatataag actgtcagca agcttttgat
     5881 tgaaacgaaa gaagatgccg aggctagtca aaactatgac cttgaagtaa cagaagaaga
     5941 tgttttagaa acacttagcc gcttgtcagg tatcccagtg gccaagctta gccaatcaga
     6001 tactaagaag tatctgaatc tggaagcaga attgcacaag cgtgtgattg ggcaggaaga
     6061 agctatttct gcagttagca gagctattcg ccgtaatcag tcagggattc gtacaggtcg
     6121 cagaccgatt ggttccttta tgttcttggg accaactggt gtcggtaaga cagagcttgc
     6181 taaagccttg gcagaggttc tctttgatga tgaatctgct cttatccgtt ttgatatgtc
     6241 tgagtatatg gaaaaatttg cggccagccg tctcaatggt gcgcctccag gctacgtggg
     6301 ctatgaagaa ggtggcgaat tgacagagaa agtccgcaac aaaccttatt ctgttcttct
     6361 ttttgatgag gttgaaaagg ctcatccaga tattttcaat gttctcttgc aggtcttgga
     6421 tgatggtgtc ttgacggaca gtaagggacg taaggttgat ttctcaaata ctattattat
     6481 catgacgtca aaccttggag caacagccct tcgtgatgat aagacagttg gttttggtgc
     6541 ccttgattta tctaaggacc acaaggaagt ggagaaacgc atttttgagg aattgaaaaa
     6601 agcctatcgt ccagaattta tcaaccgtat tgacgagaaa gttgtcttcc atagcctaac
     6661 ggaaactcat atgcaggatg ttgtcaaggt catgattaaa cctttgttgg ctgtaacagc
     6721 tgagaaagga attaccctta aactgcaacc gtcagccctt aagttattgg caaaacaagg
     6781 ttatgaccca gaaatggggg ctcgtcctct tcgtagactc ttgcagacca agttggaaga
     6841 tccattggca gagatgttgc tccgtggtga cttgactact ggttcgacct tgaaggttgg
     6901 ggttaagggt gaagagctca agtttgatgt ggtcaaatag gtggtaaaat acttgaacta
     6961 agactgcttt tagcagcaga ttgtgagggc tttatttgcc gtaatcatat agtttcggat
     7021 gagaaaggca aggtatgaat caaaccatag ctgttgtttt cggaaccttt gctcccatgc
     7081 acaaggggca tttggatttg attgagcgag caaagttgga ttgtggtcag gtttgtgtgg
     7141 tggtttcggg ctatgataag gatcgtgggg ctaggatagg tttagatttg accaaacgat
     7201 ttcaatttgc tcaagagcag tttaagggag atgatttggt gagcgtgtgt gcgcttgatg
     7261 aaaccgactt gcctccctat ccagatggtt gggacctttg gctagaccgc ctgcttggtt
     7321 tattggactt gtcagaacat caagtaccag ttttctatgt gtctgaggaa gaatacgctc
     7381 aggaactgca tagtcgaggc tatcaagcgc atttcagtcc tagaaaattt ggtatttcag
     7441 cgaccctcat tcgtgagaat ccggagaagt atcgggacta cattgccctt gcctttgttg
     7501 actttttggc tgaacaggaa taatgcgatc atacagaaaa aacacataaa atcaaattat
     7561 catcttggtt ttatgcgttt ttttgtccaa aattttttaa aagtcttatc atttttaacg
     7621 aaagttcgga aaaaacgttc acatttcaaa aaaatatttg gattttttga ctattctgac
     7681 tgattttgtt attatatact catcaaaaaa aggaggtggt tcttatgaga aagaatcttt
     7741 tgaaaaaaac ggtcgtttgt tttattaact tgcttgttgt gttgatggca gttgctccac
     7801 cagcagtgtc tgctcaagct gctagtcgaa tcgaaacacc tgttgaaaca aaatcgaagc
     7861 atttttcgaa tttagaaggt ggtggagatt tatcctatat taacctttct ggttattcaa
     7921 atagattcgg aacagcggca tctggtagat ctggtacata tttcggacca agaggaactt
     7981 atgtgactag agattggtct gttaattctg gaggaggatc acatggagga agttattgga
     8041 agctatttaa tcgaaatggt caacgaatag gtacatatga caagtatgga aattacctac
     8101 gtccatagta aataaactag gagcaatatg caaaatattc aattaaataa ttttgaaatt
     8161 aagatttcag aaagattaca ctctatttat gatggcaaaa acaagatatt agttatcttt
     8221 ccagaccata ccgcaatctg ctgtcaggta aaactttctc aagcaggcgg acttttggta
     8281 gatacatttt ggggtgtgga atatgagatt tcaggcaaca tggtaactat ctattacacg
     8341 gatgaggggg aattggaatg aaacagtatt tatttagaca gtatactatc catgttcatc
     8401 caagtttaaa tgttttcatc ggaaaggatg aagcagaaat ggttctctat agcaaagaac
     8461 ctgatttcaa acttttagcc ttgctaagat ggttaccaga taaaaattct attcgtatta
     8521 tcaaccgttg gaaattaacc tatgaatacg acggtaacaa taatatctac attcattatg
     8581 attccgacta tcgttactaa aaataagagt cgaggttcta atctatagaa aaaacacata
     8641 aaatcaggtt gcaaccctgg ctttatgcgt tttttatata tgtttcattt taacgaatgt
     8701 ctaactactc agattttctt atactcaatg aaaatcaaaa tcaggctagt tccaaaggtt
     8761 taggaaactt tggaggttgg atatagagcg aacgtagttc gtttctactt acgcagatag
     8821 aaccctgtta ctattttgtt ttaaggtaac aggctgaaag gctccactgg agccttttac
     8881 tcatcaaatc aagtcaacaa ccgctgagtt tgattttcga agagtattac ttagcaattc
     8941 gttcttgata ggcttctctg gcttggtcaa agagttcttg gacttgctca atggtttctg
     9001 ccttggcaac ggctccacgg attttggctg cacctgctga gccacggaga tagtggggag
     9061 caaggccacg gaattcgcgg acggcaactg actcgccctt gagattggtc aatcgggtca
     9121 ggtggtcaaa ggcgacgttg agtttgtcat caaaagagag gtcgggcagg atttctcctg
     9181 tttcaagata gtggttgatt tggttgaaga tatagggatt tcccatagca gtccggccga
     9241 ccatgacagc atcggctccg acttcttcaa ttcgctgacg agcatcttct acattgcgga
     9301 tgtcgccgtt tgcgatgaaa ggaatcttgg tcaggctgcc agctaccttg gtgagggttt
     9361 ctaggtcaac agttcctgtg tacatctgct cgcgggtccg tccgtgcatg gccagggcag
     9421 ccacaccggc actttctgcg gccagggcgt tttcgaccgc taggtcggta ttgttccaac
     9481 ccgtccgcat tttaacagtc aagggaatat cgaggaccga ggtcacttcc ttgatgatgt
     9541 ggtaaatctt gtcggggtcc ttgagccact tggcaccagc ttcgttttta acgaccttgt
     9601 tgacagggca gcccatgttg atatctacga tgttggcctt ggtgtttttc tggatgaagt
     9661 cggcagcccg ttttagtcct tcggcatcac caccgaaaag ctggatggac atagggtatt
     9721 cgttgtcgtc gatatggagc atatggaggg tcttctcgtt gttgtagaga aggccttttt
     9781 cagaaatcat ttccataacg actagacctg cgcccatttc tttggcgatg gtacggaagg
     9841 ccgagttggt cacgccggcc attggggcca gcacgcagcg attggggatt tccacatcac
     9901 caatcataaa aggggtattg agattagcca ttgatgagtt cctccaaatc gttttcgtca
     9961 aagtggtaat gggtctggca gaattgacag gtgatgtcca cgcccttgtc ttcgtctttc
    10021 atctcttgta ggtccgcttt tggcagacta gccagagcat cgaggaagcg gtctttagag
    10081 cagtcgcaga caaaaccgat ttcttcttct gacaggcgtt tgaagtcgtc gtcgccataa
    10141 atggcggata gcagagcttc tatgtggttt tcagaagcta gcaggcttga aatggcaggc
    10201 atttcttgga tgcgtttctc gaaacgggca atttcagcct cggtcgcgcc tggtaaaact
    10261 tgtagcaaga agccaccagc taccttgacc ttatcttctt catccagcaa tacattcagt
    10321 cccaccgcag atggtgtctg ctggctgtct gttaggaagt aagcaaagtc ttcaccgatt
    10381 tcaccagaga ttaagggtgt catggaattg taagggtggc ctgttccgta gtctgtgatg
    10441 actaggaatt gaccattgcc tacaagtggt ccaacgatga cctcgccagt cgctgttcgt
    10501 ttgtagtcca agtccggatt ttgaatgtag cccttgacct gacctttggt attggcaacg
    10561 gagatgatag ctccaacagc accgctggct aagattttaa gggtgatttt ggtatcgcct
    10621 ttttcattgg cagccaaaat ctgattggca atgagggtgc gacctagagc aacggtggac
    10681 gacgccatgg tgtcgtgttt ttcttgggct gttttcacgg tttctgtgct atctagcaca
    10741 aaggcacgga aatgcccgct ttttgatagt gttttaataa ttttatccat aactttaatt
    10801 atagcacaag tggaggtgga gcagtagaaa aatgagcctg aaagtcatgc tttcaagctc
    10861 aaaaaatgat taggattctt tttgtttttt gacaaggcag gcgctcaaga ttacgagacc
    10921 gattgtgata agtacaaagc ttgtttcttc tcctgtcttt ggaaggattt tcttttgact
    10981 gtttttggca gtaggggtca cggttgtttg tcccttgtct gtagcgccag aacggtcatc
    11041 agcatttaca gctgtttgat tagaagttga aagatttgtt ggcttgttat tgttggtcgt
    11101 accattgtca tcactattat tgactgttcc tgagtcatct gtctgcttgt cgcttggttc
    11161 tggttgagtt ggttcttgtg cctttgttac atccagttgt accaatagtg gatcgtggtc
    11221 agaagctctg ccatgttctt ccataaaggc tgaattgaca tggaccatat caaaggcata
    11281 gcgctccaac aagttagttg atactagcat attgtcaaga gactgattgt tcccgttata
    11341 gaagtaagag aagcgatcgg atgcatcgtg acgactaacg aggttagcca ttcctcctgt
    11401 ttctaaaatt tctatggttt tagtaaattc atagtcattg aagtcgccta gcataacgat
    11461 attagcattt ggattttgag ctaagcctgc ttttgtaaag tcagcgataa tttgtgctag
    11521 tatgtggcgt ttttcttcgg acttgaagct gacaggttgg atttttccgt agagaccatt
    11581 gtcaccgcgt tttgagttga gatggttggc aaggacaacg actttctcgc cgttgaagac
    11641 gaattcagct gcaagtgttt tgcggacagc tgcccaagca gggttggtag ggtcgattcg
    11701 gcccaggctg agattgagtt cgccgttttc ccatgcaact gcctgtgttg cggtaccgat
    11761 tggtttgtca gataggctga cacgttttga gttgtagagg aagccgacgc gaatattgcc
    11821 tccctcttgt ccgccgtctt tgttgttttc aggagcaatg tccacatagg tataggttgg
    11881 acctccagct gcctgaatag cagcaatcaa gcgttcagca ctcttactag catcagttgt
    11941 gccgtcgttt gtagcaccgt tgttgtcctg cacttcaatc aatccgataa tatctggaga
    12001 atgtaggtct gaaacaaagg atttggcaat gcgttggact ttagcgtctg atgttgactt
    12061 actgtcagca gaaaagtttt caatgttata agaagcgata gtcagcttgt catcatttgg
    12121 gataatggtt gttgtttctg gttttgtagc tccttcgtgc aaggttggaa gggtgctatc
    12181 atctacatag actttataat tggtgtaaga gtaggtgact ggtccagcga tacgaccggt
    12241 gaagtagtct cctgatttga caatctgttt tccatttttc aaaagtaggg gaatgatatt
    12301 ggtattattc ccttcagggc gaaggttgac gcctccaaca ttatttaaag gaagttgact
    12361 agtggcagga gtgacataga tttccttgtt ttttagtgga ccaaggattt ttgcatcatc
    12421 cacggcaacc accatgcctt caacggattc ccagaaatcg agcgcatctt gttctggatc
    12481 aaattgagcc aaaccgtcgt tgtcaataat atctgctggg atggtccgat caagaccaag
    12541 gactatagga gatggtacag gtgcagtccc attcagagtt acttcagtag ctcgaatttg
    12601 tgtgatggtt aagtctgttt tgtcacgttc agcataccct ctgccctggt attcttctac
    12661 tcgtcctgcg atagtgacaa gatctcctac cttgacattt gcttttaatt tgtcagtaaa
    12721 gatgttaatg ccgtcagatg ttttgacgtc tccatctggt gtgacatctt gcacgtagaa
    12781 gttattggca gaggtaacgt aggttactac gacattttta agcataacag attgattcac
    12841 cagaggtgac tggtgacttg caccttgaat gcgaccgact gttacagctt cggtctttcc
    12901 tagtcctgct tccgtcgttg ccttttcaat gacttcaaaa tgagagaggt caaatggttt
    12961 taattgtatt tttctattat aggtggataa aatagcggtt agattaatct tatctccctt
    13021 gttgatccgg ttcaaaagat ccgccgtttt gatacctgtt cgactatcta agcgtacatc
    13081 aacagtttgt ccttcggagt cagttacggt aaaggtagaa ttttgatagc tatcgctttg
    13141 aatatctccg acagtcaagt tttttagaga aactagtgtt gcctgtgctt gcgttgctaa
    13201 ctgagcgatg tttgtttctg taataggagt gttgaaattc tcagagatag ccttgtggtt
    13261 gctaactgtg gttagttgga gttcgccttt atactcaccc aatgttcctg ttaattggac
    13321 tgtatcacca ggctgatagc ccaaggcagc ccctgggtag atatagatac cagcaccgtc
    13381 agaaccctgg aggtaaaaac catttcctcc ccaaccgttt actaggctaa taattttacc
    13441 agaaactgtg tactctgttc cttgtgctcc ggaacgaaca gtagcaatcg gtgtagaaac
    13501 aaggtcactt ggtttggtag ctgtattttc acttgttggt ggctgaacat tagtagaaga
    13561 tgaatctaca atttcaatcg ctgtagctgg cttgatccca gctcgcttca tgtatgtatc
    13621 gctcgttcct gtgatgcgaa ccaattttcc gactagttcg ggatgatcaa tgaggttgaa
    13681 ttggctacga agaggttctt ttagttgaat agggatagtg tcggcggccg cagtatccat
    13741 gcttgcacca agggctagat ttgttttgtt agtggcgtca aaagcagttc cgctactatt
    13801 aagtggtgca ataagatagc cttgtatcgt gacttccgac ttggttattg caagtgacgg
    13861 ataattctct atcgccactt cagtagcgtg cacagatggt atgggcgaag tagcaagact
    13921 aagcaagagt ccggctgtta ccgctacaga acctacggta tagaagagag aacggtttct
    13981 aatcttcata taaaactcct ttttgttata tatggacgtc ataaaaacgt ctgcagttgt
    14041 tatatacata tagtttaaca gaaaatgagt tgtttggaat acttttttta gatataatac
    14101 ggatattttt aaaaattcaa atgaaatggt aaaaataaat tggaaaacga aacaaaaaac
    14161 gaacaaaacg gttgggtgat gggaattggt tcacaaaaaa agcaccgaga tataaatgat
    14221 tttcttggtg ccatattcat tttgtttaac attagttggg agtttttgtg aaggaacaaa
    14281 agggcaaagc agatatatag tccttttgtt gacttttttt acttcggcgc aagaggaact
    14341 ccgtttctgg atttcccgct tcaaacaatc cactggtttg tttgaactcg ctttgccgta
    14401 ctttatagag aggcaagtct tatttccaac ctagaatgtt cttgcaacct gcagctattg
    14461 aggcgaacgt gctttgccct atctccgacc ttcaaaagtt ccacgaacct ttgaggtaag
    14521 tactaacaaa gtaaggaaaa tgggaaacta tagctctgga aacaattttt tcaaaatctt
    14581 cggcgtaatg ctctgtcccg gtcctgcgta agtataggtg tgcaggtaca tggcagggtt
    14641 gaagttgagt tcgatgcagg tgcagtttgg atcttccttg ctggctggct tggtgcggtc
    14701 ggggataatc agatctacac cgcaggccca agctcccata gcagtcgcca tagcggcagc
    14761 cagttgcttg taggactcgt ccatctggtc ggtcatatca atggagtcgc caccggtgga
    14821 gatgttggaa ttgccacgca gaaaggcttg gacgccttct ggcaagaccg tatcaggtgt
    14881 gtagccctgt tgctccaaca tgagcagttc gatatcaccc agattgatga tttcaagcgg
    14941 tgatcggtga tcgcggcccc gcaggggatc ttggtttttt tgctctacta attcacggat
    15001 ggttgagcta ccgtccccca cgacattggc tgcaacgcgg agcaggacag cctcgcactt
    15061 gccatcaagg atgaagaaac ggtactcggt ccccgccacg aattcctcca ctagtacatg
    15121 gctatcttct gaaaaggcga tgtccagggc tttttcatag tctgcaaggc ttgctggctc
    15181 ctggaagata gagatgccaa ggccgaagtt ggtagatttt ggtttgacaa caatagcaga
    15241 gctggctacc tgcccgtagt accgcagggc atctgtcttg tttgcaaatt cagctcccgc
    15301 aggtactgga aaaccagcct ggtccaagat tttcttggtc acgaccttgt tggccatggc
    15361 aagaggaatg acgtagttgt ccttggaggt catgttgcca tttttgatgt actcgacatg
    15421 gtcaccatgc cagagtttga gaaattggtc ttcttcatcc aaaatctcca cattcaagcc
    15481 cagctgaatg gcgtcaaaga gaatcatctg ggtggagagt tccatgtttt catagccctt
    15541 gagagcgtaa ggggctgtcc aagcatagtc atgaaagact tgtccctgct gctgaccgaa
    15601 cttttctaga gacccatctt caacctgctc tgctattttt ccggaaaggg tcagacgagg
    15661 gtcttgaagt gctgcctcca cttgagcgat gagctggctg tagtagtcgt ccagtccaaa
    15721 gtgggcgacg atctctgtca tggcggtcag gatagggctg gcgtctgcct ctgctggtag
    15781 gggagtgtgg gggtgactga gggcgatgcg gttgttgagt tctgctgcct gagccagttc
    15841 ctcgtcaacc tgctccgagt catcaagcca aagcagagct agggcaaaga ggtgggtggt
    15901 gtcaagggtc tcttgactaa tagcaagcgg gtcatagggg ttaaggtcga aggaccggaa
    15961 ttctaaataa gaaatcccct tgctgaggta gtcacggcta gtctttgctc cacgtagtcg
    16021 gacggaagag taaaattcct tctcagcgga tagttgacca gattggacag cttgttcgat
    16081 gtcggccacg tactgttcga gagaagaaaa ggaaatatga atatcatcgg agttgacata
    16141 gccgtacttg gaattacgaa tggaacggac gcaaccaact tccgtggtta ggaatccttt
    16201 ttcggctagg ctacttgccc catataggta ggttaagagc caacgatagt tgaggaagtt
    16261 ttgtgctaat ttgaggtaga gggtattttt gaatgcgata aaatctgttt cctcgcttaa
    16321 ttcaaaaagt tgttgggtta agtctttacc tagctcaaaa ttgtagtgaa tgcctgacat
    16381 agattgaagt agcttaccgt agcggtctgc caagccgaca cggtactggt actcgtagtc
    16441 actttccagc tgagcaatct gaatctcatc ttctgagatc acaggtggca tggacagggg
    16501 ccagaggtac tcgctcttgt tcatggaccg cactgccaca tcggtaatag ctccgaggaa
    16561 acggcgggct tccttggtcg aatgagccac aggggtaatc agctccagtt gaggctcact
    16621 gtagtccgtc tggatatagg ggtgaaaact gcgtgagccc aattttttag gatggggtgt
    16681 ttgagccacg cggttgtcag cgttgatgcg taaggattcc cgttccaaac caaaggtggc
    16741 ttggaggatg ggactatttg gggataattt ctgtaacata agtaatccgt ccaatttctg
    16801 atagtgtctt acaccaattt tatctagttt gtaaaacaaa tacaaatttc tgctacgttt
    16861 ttaaggaaaa aatgaatcta ttgaatggta agaattgata ggcttcggga atctctgcaa
    16921 aaaacaaatt taaaaatgtt cagaacgagc gtttgttcgt attttgagat cgaaaataat
    16981 gcaacaagaa acaacatccg atataaacga aattattgga atttcttgaa aaaatgttcg
    17041 ttttttgata gaatagtgtg taaagcgaaa cttgcaggtg agattcctgc cagaagtaga
    17101 agaaaggctg ggctggttag ccagttcaga gaaagagtga atatgacatc agtagttgtt
    17161 gtaggaaccc agtggggcga cgaaggtaag ggtaaaatta cagacttctt gtctgctaat
    17221 gctgaagtaa tcgcacgtta tcaaggtggt gataacgctg gacacactat cgttatcgat
    17281 ggcacgaagt ataaactgca cttgattccg tcaggaatct tcttcccgga aaagatttct
    17341 gtaatcggta atggtgtggt tgtcaatcca aaatctttgg tgaaggaaat caactatctc
    17401 catgattcag gtgtgacaac agataatctg cggatttcag accgcgcaca tgtcatcttg
    17461 ccttaccaca tcaaattgga ccagttgcaa gaagagtcta aaggtgagaa caagattggt
    17521 acaaccaaca agggtatcgg tcctgcttac atggacaagg cagctcgtgt cggtatccgt
    17581 attgcagacc tcttggacaa ggagattttt gcggagcgtt tgagaacaaa cttggctgag
    17641 aaaaaccgtt tgtttgagaa aatgtatgag tcaattccga ttgagtttga cgaaatcttt
    17701 gaagaatact atgcctacgg tcaagaaatc aaaaagtatg taacagacac atctgttatc
    17761 ttgaacgatg ccctcgacca aggcaaacgt gtcttgtttg aaggtgcgca aggagttatg
    17821 ttggatattg accaaggtac ctatccattc gttacttctt caaacccagt tgctggtggt
    17881 gtgaccatcg gtagcggtgt cggcccaagc aagattgaca aggttgttgg tgtatgtaag
    17941 gcctacacta gccgtgttgg tgacggacca ttcccaactg aattgcacga tgaaatcgga
    18001 gaccgtatcc gtgaaatcgg taaagaatac ggtacgacaa caggtcgccc acgccgtgtc
    18061 ggttggtttg actcagtggt gatgcgccat agccgccgtg tgtcaggtat taccaatttg
    18121 tccctcaact cgattgacgt cttgtcaggt cttgagacct tgaaaatctg cgtggcttat
    18181 gacttggatg gtgagcgtat tgaccactac ccagcaagtt tggagcaact caaacgttgc
    18241 aaaccaatct atgaagaaat gccaggttgg tctgaagaca tcacaggtgt acgtagcctg
    18301 gatgaattgc cagaagcggc tcgcaactat gttcgtcgta tcagcgaatt ggtaggcgtt
    18361 cgtatctcaa ccttctcagt aggacctggt cgtgaacaga ccaatatcct tgagagtgta
    18421 tggagcgcaa aatagtgtgc gaagactgaa cgaaagttcg gtctttttct gtttggtcga
    18481 actttgcttt cttgtccaga atttgatata atcatgctta tcaaacaata aaagaggtaa
    18541 acaaatggaa ggtttatttt tgccgcttgt catggttgcc atgattggtt ttatgttcta
    18601 ttcgcaacgt aaacaacaaa aacaacgtca agaagcgcta aatcaaatca aaaaaggtga
    18661 tgaaattgtg accattggtg ggctatttgg tattgtcgat gaaatcgatg ataaaaaggt
    18721 tgttctcgat gttgatggtg tttatttgac atttgaacgt ggtgctattc gtaatcgtgt
    18781 agcaagttca ggtactacat ctgcagttgt tgaagaagcg gcagttgaaa cgacagaagc
    18841 gactccagaa tctgcaattg aagaataagg atgaaaaacc gagggaaacc ttggttttct
    18901 cttgttttag ccctgcctga tttttgctat cctaggtaaa aatatggtat aatgaaacga
    18961 ttatacaata aggaaaatac catgtttaaa tttaatttta aaaagaaaga aagcattgaa
    19021 actgcaattg aagtccccaa gcatatcgct gtgattatgg atggcaatgg ccgttgggcg
    19081 aaaaagcgga tgcagccacg tattatgggg cataaggctg gtatggatgc acttcaaaag
    19141 gttaccaaga cggcttctga tttgggagta aaggtgttaa ctgtctatgc cttttctaca
    19201 gaaaactggt ctcgaccgga aaaagaagtg aagtttatca tgaatctacc tgtggagttt
    19261 tatgataagt atgttcctga actccatgcc aataatgtca aaattcagat gattggcgac
    19321 cattcaaaac ttcctgtccc gactctcaat gcgctttata aagccgaaca aaagacaaag
    19381 agtaacactg gtttgattct caatttcgct cttaactatg gcggtcgtga tgaggtgact
    19441 cgcgcagtca aggctattgc gcaagatgtc ttggatgcca agttcgatcc tggagatatt
    19501 gatgagaaat taatagcaga ttacctctat acaggaacct tatcaccaac gttacgcgat
    19561 cccgatctag ttatccgtac aagtggtgaa cttcgtctta gcaacttcct accgtggcaa
    19621 acagcctaca gcgagctgta ctttacagat attgcttggc ctgattttga tggagaagca
    19681 cttaaattag cgattaaaga atataaccgt cgccacagac gttttggcgg tgtataagga
    19741 gaaggtatga caaacgattt acagaaacga gtgatatttg gcggcattgc tctagctatt
    19801 ttcattccct ttttgttggc tggtgggatg gcatttcaat tttttgtagg cctgttagcc
    19861 atcattgcta cggctgaatt gattaaaatg catcgcctag caccaaattc tatcgagggt
    19921 gtcttagcta tgttggccag tttagtcttg gctcttccgc tccagaatta tctgacattt
    19981 ttaccgacgg atggaaatta tacagcctac gccattgtag tatttttact gcttggttcg
    20041 acggttttta atatcgatca ttacaactat tccgatgtgg tctttccaat tgcatcaagt
    20101 ttctatgttg gtatcggctt tcatagtttg atactggctc gtatggatgg gctcaataaa
    20161 gtcttctttg ccctctgttt agtctgggca acagatattg gtgcctactt aatcggtcgt
    20221 cagattggtc ggagaaaatt attgccgaag gtttcaccga ataaaacggt tgaaggcttt
    20281 gtaggcggta ttctttctgc ggtggtagtt gctatcattt tcttgattgt cgataagtcc
    20341 ttgttggcgg gctattcatt tggcatgatg atgttgttag ttgtgatttt ctcgatattc
    20401 tcacaattcg gagatttagt agaaagtgct attaaacgcc attttggtgt gaaggattct
    20461 ggaaaaatta ttccaggaca cggtggtatc ctggatcgtt ttgacggtat gatttttgtc
    20521 tttccaatca tgcacttctt tggattattt taaggaggta ctatgaaggg aattttagca
    20581 tttatattta tatttggtgt gattgtggtt gtccatgaat ttggtcattt ctactttgcc
    20641 aagaaagctg gtatccttgt gagggaattt gcgattggga tgggtcccaa aattttcgct
    20701 catacaggta aggatgggac actttacacc atccgtatcc ttcccctagg tggctatgtt
    20761 cgaatggctg gatggggtga agataagaca gagattaaga caggtagtcc tgccagcctc
    20821 agcttgaatg aagctggtgt tgtgacgcgg attaatttgt ctggtaagca actggatagt
    20881 ctcagtttgc caatgaatgt aaccagtttt gacttcgagg aaaaattgga aatcacaggc
    20941 ttggtcttgg aagaaagcaa gacctataag gttgatcacg atgccacgat tgtagaggaa
    21001 gatggaacag aagttcggat tgctccactt gatgttcagt atcaaaatgc gacagtctgg
    21061 ggacggttga tgaccaactt tgcgggtccc atgaataact ttattttagg gattctagta
    21121 tttattattt tgttcttcat gcaaggcggg gttgccaacc catcaagcaa tgcagtaagt
    21181 attactgaag gtggtgcttt acaggaggca ggtgttgtca caggggacaa gattctttcg
    21241 gtcaatggga acacgacaga cagctacaca gaagttgcaa cgattattag caaagcggcg
    21301 acagatgcaa caacagcccc aagctttgat ttggttgttg agcatgatgg gaaaaaccgt
    21361 catgtctccg ttactgctga acaagtagat ggggcctatc gaattgggat ttcaccgatt
    21421 ttgaaaactg gtttcgttga taaaattgtc ggtggtttcc aagaagcggg ggcgacagct
    21481 cttagggttg taacagcttt gaagaatcta attgcaaact ttgatgttaa gcagttgggg
    21541 ggaccggttg ctatttataa agtaagttct caagcggctg aatttggttt ggtctctgtg
    21601 ttggggctga tggctgcgct ctccattaac cttggaatct tcaatttgat tccaattcca
    21661 gccttggatg gtggtaagat tgtgatgaat atcctagaag ccattcgcag aaaaccactc
    21721 aaaccagaga cggaatctta tatcactcta gcaggtgtag ccgttatggt ggttcttatg
    21781 atagtagtca cttggaatga tattataaga gtcttcttct aagataattt gaaatttatg
    21841 aaaggaattg aatgcgtgct atacgcagga tgttctgaaa aatacgaaaa agaaagtaac
    21901 agagagtttt cagatttgaa ctcgagcgtc agtgaggggt ttgaaaagtt aattctttgt
    21961 cttccaatca agtaaactac gaaaggaatt gaacacgggc tgagaccgtg tcttcctgtc
    22021 aaacaaatga tgaaaggaat tgaatgcgtg ctaaaaactt tacgaaaaag ataaacctgc
    22081 ctagtgccca aggcactgcg ttaggtttcc tattttcgtt tcgttttctt aacgcactta
    22141 tatctatctt aactatgaaa cagtctaaaa tgattatccc tactttgcgg gagatgcctt
    22201 cggatgcgtc ggtgattagc cacgctttga tgttacgtgc gggttatgtc cgtcagattt
    22261 ctgctggtat ttacagctac ttgccattgg ctaaccgtgt gattgaaaaa gcaaaaaata
    22321 tcatgcgtga ggagtttgac aagattgatg ccattgaatt tttggcacca gctctcttgt
    22381 cagcggacat ttggcgtgag tcgggtcgtt acgaaacata tggcgacgat ctttacaagc
    22441 tcaaaaaccg tgagggttct gactttatct tgggtccaac ccacgaagaa acggtgacac
    22501 tattggcgcg tgatgcggtc cagtcttaca agcagttgcc acttaacatc taccaaatcc
    22561 agccaaaata ccgcgacgaa aaacgtcctc gtaatggtct cctccgtggg cgcgagttca
    22621 tcatgaaaga tggctacagc ttccacgcta gctatgagag tctggaccag acctacgatg
    22681 actacaaggc ggcttacgag gcaatcttca ctcgtgcagg cttggagttt aaggccatta
    22741 tcggtgatgg tggtgcaatg ggcggtaagg atagtcaaga atttatggct atcaccccag
    22801 accgtaccga tctaaatcgc tgggttgtct tggataaatc ggttgcttct tttgaggaaa
    22861 tcccagagga tgtccttgaa gcgattaaaa cagaactctt ggcttggtct gtatctggtg
    22921 aagatactat cgcttactca agcgaatctg gctacgctgc caacttagag atggcaacga
    22981 gtgagtacaa accacggaca gctgtggttg ctgaagagac attagtaaaa gttgctacac
    23041 cagatgcaaa aacaattgat gaagtggccg ctttcttgaa tgttgcggaa gagcaaacca
    23101 ttaaaaccat gctctttatg gcagatgggg agcctgttgt tgccctactt gttggcaacg
    23161 accaggtcaa tgatgttaaa ttgaaaaatt accttggagc agatttcttt gatgtagcag
    23221 gcccagcgga tgctgaaaaa atcttcggtg caggttttgg ttcccttgga ccagttggct
    23281 tgccagaaaa catcaagatt atcgcagacc gtaaggtaca agatgtgaaa aatgctgttg
    23341 ttggcgcgaa cgaggatggt ttccactata caggtgctaa tgcgggacgt gatttccaag
    23401 tgactgagta tgtggacatt cgcgaagtca aagaagggga gccatcacca gacggtcacg
    23461 gggttcttaa ctttgcccgt ggtatcgaaa tcggtcatat tttcaaactt ggtacacgct
    23521 attcagatag catgaatgcc aacatcttgg atgagaatgg tcgttctatg ccgattatca
    23581 tgggatgcta tggaatcggt gttagccgcc tcttgtcagc cgttcttgaa caacacgctc
    23641 gcctctttgt taacaagaca ccaaaaggcg aataccgtta cgcttggggg atcaacttcc
    23701 ctaaagaatt ggcaccgttt gatgtgcatt tgattccagt taatgtcaaa gatgaagcgg
    23761 cgatggaatt gactcagtct atcgaagcaa gtttggttgg tgcaggctat gaagtattaa
    23821 ctgacgaccg taatgagcgt gtcggcgtta agttttctga cagtgacttg attggcttgc
    23881 caatccgtgt gactgttggt aagaaagcag ctgatggcat cgtcgaagtg aaaatcaaag
    23941 gtacaggtga tacggttgaa gtccatgtgg accaactcct tgaaaccttg caaatcctta
    24001 caaaagaaaa tgaataacag aaaaagactt ggttgatgat agccaagtct ttgttgataa
    24061 aatgggagtg ggaaaggact cgactaggtc aaaaagagtt cgttttccca cccccgcaca
    24121 gttgattagg tcagatttgg agtgtgaaac acgaataaat ctgccaatca accactgcgc
    24181 tgagatgttg atacgaactc tgataagttg tgctgggctt tttccaaaag cctatttttt
    24241 tataacatat ttagtaggag gttatagagg atagctcctg cgactcctcc aagaattggg
    24301 ccagcaactg ggatccagct ataagaccag tcagaatctc ccttgttagg aattggaagg
    24361 atagcgtgca tgatacgagg gcctaagtca cgagcaggat tgactgcgta gcctgtcgtt
    24421 gatcctagtg agaagccgat tgcgaatacg ataagtccca cgatgattgg gccgaggcca
    24481 gcgctgacat tgttaggacc aattgcaagg attcccatga ctaagacagc tgtaccaaga
    24541 gcctcgccga gcaagtttga agctgtgtga cgaagagctg gtccagtaga gaaggtagca
    24601 aggattgttc cgctatcttt tgtttcctgc caatgtgggt agaaatggag atagaggacg
    24661 atggatgcca acattgctcc agcaaattgg gcaagtagga agcctgggac ttgagcccat
    24721 ggtagggatc cgatagatgc cattgcgata gtgacagctg gattcaggtg ggcaggtcca
    24781 aaaattcctg atatgtagac ggcaattgtt acagcgatgc cccatcctag tacgatggtc
    24841 agccagccgc tattttcagc cttggtttta gagaggatgc aggatgcaac cacaccgttt
    24901 ccaagtaata ctagtatgaa agttccaagt aattctccaa tcagattcat aatgacctcc
    24961 tatttattga ttgtcagttc ttttaattgt tcaagaatag tctccaagtc agctccagcc
    25021 tgtaagagag ctgcggcagt atagcaacct tcgacgactg gtacggtttg gataagaatt
    25081 tgtttgtctg taaaatcagc agcaagttcc atgttcattc gggcgctacc taggtcgaaa
    25141 aaggcaagga gggtatcagc agggtttgct tcaactcgct cttgtacgat ttcaaagctg
    25201 gtaccgatgc taccatcttc tagtccgcca cagtaggtga tgggaatatc ttttgcgact
    25261 tcagatacta ggtcgatgat tccctgggcc aaattttttg aatgggatac gagtaagata
    25321 ccaacctttg tcatagtcct gcctccaaca tggcttcaaa gagaagtcca gatgagaaag
    25381 agcctggatc aatgtgtcca atagaccttt ccccaacata ggacgcgcgt cccttggttg
    25441 cagcaatatc ctttgtccct tgaacggctt tatggataat ttccgttgtc agctggcttt
    25501 ctcttagggc agcaatgacg ggaatccaaa cgtctaccat ggttttctcg cccaattcag
    25561 ccttgccgcg tttttggatc atgtccaaac ctgcttgaag cgtatctgcc aaatcagcgc
    25621 cagcttgact ggctttagta atacccataa aggcagagcc gtagagagga ccggaagcac
    25681 cgccaacctt gctgagaagt tgcatggcaa ccaccttaaa gacttggtca gcattctcat
    25741 agtctttttc agatagctcc tgcatgacgg cagccattcc tcgcgccatg ttgcccccgt
    25801 gatctccgtc tccgattgga gaatctagct cactcaaata atctttgttt tcttgaattt
    25861 ttttgttgaa tgtctccatc cagagcagtg cttgttttgc atccatagtc tttctcctac
    25921 caagctacca cttcaacagg ggcctgcaaa gcgtcaatcc agcttgggtc ttctagtttg
    25981 attaaggtca atgacagtcc agccatatca atcgatgtca tgtagttacc gattttcttg
    26041 tagtcaatga ccacaccttt tttagccagc aattgagcaa cgtcgttggc aaagacatat
    26101 tgttccatga gaggggttgc gccgagaccg ttgataagga caccatagtg ctcgccatct
    26161 tgaatatcaa agccttcagc caatttctca accaattctt cagccaattt agcagatggt
    26221 tggagttttt ctttcttata gccaggttca ccatggatac cgacaccgta ttcaaattca
    26281 tcctcttcaa ggacgaagcc tggtttgccc acttctggta ccgtagcacc tgatagtgct
    26341 aagccgatag tcttgatttg tgggacgaga cggtcagcca gttccttgat ttctgctagg
    26401 gatttgccag tttctgcagc atgaccgaga atcttatgga caaggatggt accagctaca
    26461 ccacgacggc cttgggtata gagactattt tccacagcga tgtcatcgtc cacgatgaca
    26521 ctggccactt cgatgccctc catttccgcc aattcttgtg ccatctcaaa gttcatgatg
    26581 tcgccggagt agtttttgat gaccataaag acgccagctc cctcgtcagc agccttgatg
    26641 gcttccaaaa tttggtctgg tgttggtgag gtaaatactg cgccacatac agcggcggac
    26701 aacatgccct tgccgacaaa gcctgcatga gaaggttcgt gaccagagcc acctcctgag
    26761 atgatgccga ctttaccagt tttttcagcc ttgcggataa tcacatcaaa tccattgata
    26821 cgattgacaa gatcttggtg cataaagaca agccctttta acatttcatc aacgacatgt
    26881 tcgggttgat tgataatttt tttcatgaga aaactccttt gttttttgtt tgcgctttca
    26941 ttctacattg tccaaaagaa aaaaggaagg gacagattgt ccaatgtgtc ccttttgttc
    27001 caaagaaaag catgataggg tataataaag aggaagcgaa aggaagtgac atatgccaac
    27061 ctcgttgatt acaaagaaaa ggattgcaaa ggctttcaaa aaacagctat ctcaaaaatc
    27121 ctttgataaa atctcagttg tcgacatcat gcaagaagca gggattcgtc gtcaaacctt
    27181 ttacaaccat tttttagata agtatgagtt gttagactgg atatttgaga cagaattgca
    27241 ggagcaggtt acagataatc tgacctatat tagtggaatc aagcttctgg atgagctctt
    27301 gtactacatc gagagtaacc aatctttcta tagacaactc tttgagatta aggggcaaaa
    27361 tgattttgtc agctatttag aaggctactt tatggttttg atggagaaaa ttagcaatga
    27421 gtaccagctc caggagggta aaatgatttc ccaacaggat aagcattttt tgattctcta
    27481 tcatgctaat gcttggattg gactgataaa ggaaggactg acgcagtcgc cttgtcagat
    27541 tcacagctat gggaagcaaa tggtggctct tgttcgcgag tcgtttttga ttttgtagga
    27601 gttaatgtat ggtgtttatt agaaatagac aggcaacaat tattgaagat tatttggatg
    27661 gtttctgtcg aactaatcct aaagtgaaga aagtgaaggg gcttccgatt gtgcttcaga
    27721 aagtgcagga tgaccattta gttccaatta tttcaggtgg tggatcgggg cacgaaccgg
    27781 cacatattgg atttgtcggt aaaggaatgc tgactgcagc agtatatggt gaaatattta
    27841 cgcccccaac cgctgaagaa gtattggcag ccattcgtgc agtagataag ggcaagggag
    27901 tttttctgat tgtaaaaaac tttgaggcag acctagtctc ttttagaaaa gctatcgaat
    27961 tggctcggca ggagggaatt gcagtcaagt acatcgtatc tcatgacgat atttcggtcg
    28021 atactaagaa taatttccgt atgcggcata gggggcttgc agggaccatt ctacttcata
    28081 agatattagg agctgctgcc cttactggct atagcctaga tgaattggaa cagctgggac
    28141 tgtctctggc aacagagatt gcgaccatcg gttttgcaac caagtcggcc tatttgccca
    28201 atgctgccct gcctttgttt gatttggagg agggacaaat atcttacggc atcggtatcc
    28261 atggagaaga aggctatcgg accgttcgtt ttgaatcctc tgagcagtta gccaacgaaa
    28321 ttgtgaacaa attaaaatta aaattccgct ggaaggaggg ggaagactat attcttctgg
    28381 tcaataatct tggatctatg tctgaggttg agcaaggaat ctttttgaat gacatttgcc
    28441 aatttctaga gctagaaggt ttacagcttt cctatataaa aacaggtaaa tttatgacca
    28501 gtcttgatat gggaggaatt tctgtcaccc tctgtcgtgt taaggatcga aaatggctgg
    28561 actttttaga ggcgcctaca gaagctgcgg cttggtaagt taagtatcaa atgatgaacc
    28621 tcttttggaa tgccgaagag gttttttctc tctcccaaga aaatttcctc catttatggt
    28681 aaaatgtttc taacgaacta caaaggaaat aagtatgtca gataagtttc aactcttgct
    28741 acaacaaatc ggaattcctc ttgatgcacg acaatcaggg gctttttcga ctgcaacgat
    28801 tgagaaagta gtcttgcaca aggtcagcaa actttgggaa tttaccttcc gttttgaaac
    28861 ccccttgccg ctcatggact atcaactctt caaggctcgt ttggcgacgg agtttgaaaa
    28921 ggtgggcaat aagattcaat tttctattgt cagcgatgcg gaggcttttg aagctggttt
    28981 ggtagaagca tattatccag aagcttttac ggaagatttg tgccagagtg caggcttcaa
    29041 ggctcttttt cagccattag aggtgactta tagagatggt gttttgtgga taaaaggtcc
    29101 tgaaaccatt gacacagatc attttcggaa gaatcatctg ccaaatcttg tggagcaata
    29161 caagcgattt ggttttggta atcttgcggt ggacatccaa gtctgtcagg aaatgacgca
    29221 gcaacaagcg gagattttcc atgcgcaaaa tgaagaaatt taccagcagg ctaatgaaga
    29281 aaacttagcg gcccttgaac aactggctca aatggtgcca ccaccagagg cagctcagcc
    29341 atttgtccca gaatataaga aaaatcgtcc tgccaaggtc aatatcgaaa aggctgagat
    29401 tacacctatg attgaggtgg atagcgagga aaatcggatt gtctttgagg gtcttgtctt
    29461 tgaggtcgag cagaagacga ccaagacggg gcgggtcatt atcaacttta agatgaccga
    29521 ctatacttcg tccttcacct tgcagaagtg ggctaagaat gaggaagagg ctcagaagtt
    29581 tgatatggtc aaaaagggca actggctgcg ggtacgaggc aatgtggaaa ccaataattt
    29641 cactcgtgat ttgactatga acgtacagga agtccaagag gtcaagaagg aaattcgtaa
    29701 ggaccttatg cctgagggtg agaagcgggt cgagtttcat gcacatacca acatgtctac
    29761 catggatgcc ctgcctgccg ttgaggattt ggtggcgcgt gcggcagcct ggggacacaa
    29821 ggcggtggcg attaccgacc acggcaatgt ccagtccttc ccccatggct accatgctgc
    29881 ccgtaaagct gggattaagc cgctcttcgg gatggaggct aatattgtcg aggactctgt
    29941 gcccattgct tacaacgaag cagatgtagt cctgtctgat gccacctatg tggtctttga
    30001 cgtggaaaca accggtctgt ctgcagtcaa caatgccctg attcagattg cggcgtctaa
    30061 gatgcacaag ggcaatatca ttgcagaatt tgatgagttt atcgatccag gtcatccgct
    30121 cagtcaattt acaacagatc tgacaggaat cacagatgag catgtgcgtg gttctaaacc
    30181 tctagaacaa gtcctgcgtg agtttcaaga tttttgtcaa gattccgtca tggttgccca
    30241 caacgcgacc tttgacgttg gctttatgaa tgtcaactat gaacgagcag gcttacctat
    30301 tattagccag cctgtcattg ataccttgga atttgctcgt aacctttacc ctgactttaa
    30361 gcgtcatggt ttaggtcctc tcaccaagcg gtttggtgtt gctcttgaac accaccacat
    30421 ggctaactac gatgcggagg cgacagggcg cctgctcttc atcttcctaa aagatgcctt
    30481 ggagaagcac aatttgacca atctcaatca gctaaatacg gagctgattg cggaggattc
    30541 ctataagaag gctcgtgtca agcatgccac gctctatgtg attaatcagg ttggtttgaa
    30601 aaatatgttc aaactggttt ctctttccaa taccaagtat tttgaaggag ttccacgtat
    30661 tccacggacc gttctcaatg cccatcgtga aggcttaata ttgggaacag cctgtcaaga
    30721 gggcgaggtc ttcgatgact tgctctccaa agggatagat gaagcggtta aaacggcagc
    30781 ctattatgat tttatcgaag ttatgccacc tgccctctat gctcccatga ttgccaagga
    30841 gcagtttaag gacatggcag agattgaaga gactataaag cagttgattg aggtaggacg
    30901 tagggcaggt ctgcctgttc tggcgactgg aaatgtccac tacattgacc cagaagagga
    30961 aatctatcgg gaaatcatag ttcgtgctct aggtcaaggg gcaccgatta actggaccat
    31021 cggaaatggt gagaatgccc agccagcacc actgccaaaa gcccacttta gaacgactag
    31081 cgagatgttg gacgagtttg cattcttggg tgaaagtctg acccgagaga ttgtcattac
    31141 caatcccaat gccatgctgg atcggtttga agatgttcag gtggttaaga ccgacctcta
    31201 tacgccttat atcgaaaagg ccgaggaaac ggttgcggaa ttgacctatc agaaagcctt
    31261 tgaaatttat ggcaatcctc ttccagatat catcgacctg cgaatcgaaa aggaactgac
    31321 ttccatttta ggtaatggct tcgccgtgat ttatctggcg tcgcagatgt tggtgcaccg
    31381 ttccaacgag cgaggctacc tggtgggttc acgggggtct gtcggctcca gtttcgttgc
    31441 gaccatgatt gggattaccg aagtaaatcc tatgcctcct cactatgtct gtccaaattg
    31501 tcaacacagt gaatttatca cagatggttc ctatggttca ggttttgacc tgccagacaa
    31561 ggattgtgaa aagtgtggga ccaagtataa aaaagatgga caggacattc ctttcgagac
    31621 cttccttggt ttcgatggag acaaggttcc cgatattgac ttgaacttct caggggatga
    31681 ccaaccgtct gcccatttgg atgttcggga tattttcggt gaagaaaatg ccttccgtgc
    31741 aggaacggtt ggtacggtgg cggccaagac ggcctatggt tttgtccgag gctatgagcg
    31801 ggattatggc aaattctata gagatgtgga agtagaacgc ctggctgctg gagcggctgg
    31861 tgtcaaacga acgactggtc agcacccagg gggaattatt gtatttcctg actacatgga
    31921 tgtctatgac tttacccctg ttcagtatcc tgccgatgat gtgacagcca gctggcagac
    31981 cacccacttt aacttccacg atattgatga aaacgtcttg aaactcgata ttctgggaca
    32041 cgatgatcca accatggttc ggaagttgca ggacttgtca ggcatcgatc cgcaaactat
    32101 tcctgctgat gataaggggg ttatggctct tttttctggt acggaaattc tgggtgttac
    32161 cccagagcag attgggacac cgacaggtat gctgggcatt cctgaatttg gaaccaactt
    32221 tgtacggggg atggtggagg aaactaaacc gaccactttt tcggaactct tgcagttatc
    32281 tggtctatcg cacggtacag acgtttggtt gggcaatgcc caggacttga ttaaggcagg
    32341 aattgctaat ctatctactg ttatcggttg tcgggatgac atcatggttt acctcatgca
    32401 cgcaggtttg ccacctaaga tggcctttaa tatcatggag cgggtgcgga agggcttgtg
    32461 gctcaagatt tctgaggaag agcgaaatag ctacattcaa gccatgaagg ataacaaggt
    32521 gccagactgg tacatcgaat cctgtggaaa aatcaagtat atgttcccta aagcccacgc
    32581 ggctgcctac gttatgatgg ccttgcgggt tgcctatttc aaggttcatc acccacttta
    32641 ctactattgc gcttatttct ctattcgggc caaggcattt gatttggcaa ccatgtccgg
    32701 tggcttggaa cgagtcaagg ctaagatgga agaaatcgcc ctcaagaaga aaaataatga
    32761 agcttcgaac gttgagcagg atctctatac cacacttgag ttggtaaatg aaatgctgga
    32821 acgtggcttc aagttcggga aattggacct ttacaagtcc catgcgaccg atttcttgat
    32881 tgaagatgat accctcatcc caccatttgt tgccatggat ggcctgggag agaacgttgc
    32941 taagcaggtt gtggcggcgc gtgctgaagg agaattcctc tccaagacag aactccgtaa
    33001 gcgtggagga ttgtcaggta cactggtcga aaaaatggat gaaatgggca tcctcggtaa
    33061 aatgccagag gacaaccagc tcagtctctt tgatgatttg ttttaaactt gttcactgtt
    33121 tcttttggaa gcagtgaatt tttttataaa aagaagtcaa aactcttgac taatgttttt
    33181 gataggtata taatctctac atagtgataa aaactactat gtagtaacaa taagaaaaga
    33241 ggaaatccta tgtcaaactt tgcaaaactt caagaaactc gccgtacaat ctacgcactt
    33301 ggtaaagact tgccagtatc aaatgaagaa gtggtagcac ttgttgagaa agccatgaaa
    33361 gaagctccat cagctttcaa ctcacaatca agccgtgcag tagtactttt tggtgcggag
    33421 tcagaagctt tctggaacga aattgcctac agcgaattgg aaaaagtaac gcctgcagaa
    33481 gcgtttgaag gcacaaaagg tcgtttggct agctttgcag caggtgcagg tactatcttg
    33541 ttcttcgaag accaagacgt ggtgaaaggc ttgcaagaga gcttcccgct ttacgctgaa
    33601 aacttcccaa tctggtctga acaagctcac ggtatcaact tgtacgcagt atggttggcc
    33661 tttgctgaga aaaatatcgg tatgaacgta caacactaca acccattggt tgatgcacaa
    33721 gttgctgaaa aatacggcat tccagctaac tggaaactcc gggcccaagc accattcggt
    33781 agcatcgcag ctccagcagc agacaaagac tatatggcgg atagtgaccg tgtaaaggtt
    33841 tttggtaact agtaaagttt acagtttgct ttattacgtc ggcagatgag gaaacttcgt
    33901 ttccttattt ccaacttcaa acaatccact ggattgtttg aactcgcttt ggcgtacgtt
    33961 acgagcgtta cttactatcg taagtaagat ttccaacctt caacagtcac taccctgact
    34021 gttgaagcag cctgcagttg ttgacgggag cgggaaagaa ctcgaccatt catagaagag
    34081 ttcgtcttcc cgcccccgca cagttgatta ggtcagattt ggagtgtaaa acacgaacaa
    34141 atctgccaat caaccactgc gctgagatgt tgacactaac tttgagaagc ggtgctgggc
    34201 tttttgccca gcctctttat ctcccacctc taactatctc ccagatagtt agagctagta
    34261 ctaaaaacaa actgaaaact ttaccctgcg atgggatcag acgttgttga cttgatgtgg
    34321 tgagtgtaaa gctccagtgg agttttacag cctgttccct taaaacaaga gaggagcagg
    34381 gttatgtcta cggcttgctg acacgaactc cgttcgtttc atcttcaagc ttcaactatc
    34441 tcccagagct aaccttttca acttcctgtg tcagcaggaa gtttttttat ctgtctagct
    34501 taaaatatga tacaataata gggtatttca aagtacaaag gagaaaaaga tggtattacc
    34561 aaattttaaa gaaaatctcg caaaatatgc taaactcttg gtttcaacag gtatcaatgt
    34621 ccaacctggt cacactgttc aattgactat cggtgtagaa caggctgagt tggctcgttt
    34681 gattgtcaaa gaagcttacg cccgcggtgc taaggaagtc ttggtcaact ggttggacga
    34741 tgtgattgcg cgtgagcgtt tggttaatgt cgatgtggaa ttcttggaac aagttcatcc
    34801 acaacgcatc actgaaatga actacctctt ggagcgtaag gccagccgtt tggtggtctt
    34861 gtcagaagat ccaggtgctt acgatggcgt tgatccagag aaattgtctc gcaacgctcg
    34921 tgcactcagc caagctttga acccaatgcg gcaagcatct caagctaaca aaatcagctg
    34981 gacactcggt gcagcctctg gtttggaatg ggctaaaaaa gtcttcccaa atgcagcgtc
    35041 tgacgaagag gcagtggacc tcctttggga ccaaatcttc aagacttgcc gtatctacga
    35101 ggaagatcca atcaaggctt gggaagagca cgaagcacgc ttggtggcta aggctaaggt
    35161 ccttaacgac gagcagtttg tcaaattgca ctacacagca ccaggaactg acctcgttct
    35221 cggtatgccg aagaatcact tgtgggaagc ggctggttcg gttaatgccc aaggtgaaca
    35281 ctttattgcc aacatgccaa cagaagaggt cttcacagca cctgactacc gtgtggcaga
    35341 cggttacgta acgtctacaa aaccactcag ctacaacggt aacatcatcg aaggcatcaa
    35401 ggtaaccttc aaagacggtg aaattgtgga cgtgacggct gaaaaaggcg atgaagtcat
    35461 gaagaaatta gtctttgaca atgctggagc gcgtggtctc ggtgaagttg cccttgttcc
    35521 agacaagagc ccaatctctc aatcaggtgt gactttcttc aacaccctct ttgatgaaaa
    35581 tgcgtcaaac cacttggcga ttggtcaagc ttatgccttc tctatcgaag gtggtacaga
    35641 aatgagccaa gaagagctca aagaagcagg acttaaccgt tcagatgtcc atgtggactt
    35701 catgattggt tctaacaaga tgaacattga cggaatccgt gaagacggta ctcgtgtgcc
    35761 aatcttccgt gacggtgagt gggcgattta atgatatgtg tataagactg ggcaaccagt
    35821 cttattttgt aagaatagga ggaaaacaag atgttaacaa gtattatcgc aggtgccatt
    35881 attggcttga ttgcaggcgc tcttacttca tcagatgacc gtcgtggttg tcttggaaat
    35941 atcatccttg gacttgctgg ttcgtggatt ggacagttgc tctttggtga ttggggtcca
    36001 cagtttgctg gtatggccct ggtaccgtca gtctttggag cagtattgct ggtggcaatt
    36061 tttagcagtt ccagacggaa ttaaacacct gaaccgtgct tcaattggag catggttttc
    36121 tattatctgg atttgtacga attgctgcat tgttcggatt tttcagaaaa tttccatttt
    36181 tctgttgaca agtgaaaata agtagggtat catagtaaac aattaaaaca ctgaaaggaa
    36241 aagtcaaatg aaacctgtca ctacaaaatt ctttgctttg ttgttgcgac ggtcgggcta
    36301 gtgctgaaag aatgcattag tcctgtttgg cttaccaagc gggcgctgtt gacaacatct
    36361 cgcgggtagt tttcctcgag gtgttttctt tttcttatgg ttgctttgaa atctaagaaa
    36421 gagaacctaa tattgacgtt agggttattt agaaaggtat tcttatgcgt gtgattgaat
    36481 ttttagatac aactttgcga gatggggaac agacgccagg tgtgaatttt tctatcaagg
    36541 aaaaagtgac cattgccaag cagttggaga aatgggggat ttctgctatt gaagcgggtt
    36601 tcccagcggc tagtcctgat tcgtttgaag cggttcgtca gattgcggca gcgatgacca
    36661 agacggctgt gacaggtttg gcacggtcag taaaatcaga cattgatgct tgttatgagg
    36721 ctctgaaaga tgccaagtat ccgcagatcc atgttttcat tgcaaccagc cctattcacc
    36781 gtgaatttaa actgcaaaag actaaggaag aaattttggc gcaaatcact gaacatgtca
    36841 gctatgcacg tgagcgtttt gaggtggtgg agttctcgcc agaggatgcg acacggacgg
    36901 agttggatta cttgcttgaa gtggtgcaga cagcggtaga tgctggtgcg acctacatca
    36961 acattccaga tacagtcggc tttacaactc ctcagcatta cggagaaatt ttccgttact
    37021 tgacaacaaa cgtcaaatcg gaccgtgaga ttattttcag tccgcattgc cataatgacc
    37081 taggtatggc tgttgccaat accctttcag ctattaaaaa tggtgccggt cgtgtcgagg
    37141 gaactatcaa cggtatcggg gagcgggctg gaaatgcggc ccttgaagaa gtagcagttg
    37201 cccttgaaat tcggaaagat ttctatgatg tgactagtcc gattgttctt aaggaaacgc
    37261 tcaatacttc ggaattagta tctcgcttct cagggattgc cattccgcgc aataaggcgg
    37321 tggtcggtgg aaatgccttc tcacatgaat ctggtatcca tcaggatggt gtcttgaaaa
    37381 atccattgac ctatgaaatc attacacctg agttggtagg ggtcaagaaa aattctctgc
    37441 cactgggtaa actatctggt cgccatgcct ttgtagaaaa gctgaaagaa ctagacctgt
    37501 attttgaaga aggagacgtt gctagcttgt ttgttcgttt caaaaacctg gcagacaaga
    37561 aggaaaaaat tacggatgct gatattcgag ctctagtagc tggtacagaa attagcaacc
    37621 gtgatggctt ccaatttaaa gatttgcgtt tggatagtca gtctgacgga agtattcagg
    37681 ctcaggtcat cttcatcaat caggatgagg aagaagtggt tgttgcagag tcaggcaagg
    37741 gttctgttga ggctgtcttt aatgccattg accaattctt ccaccaagag attagcttgg
    37801 aacgctacca tattgacgcc atcaccgatg gaattgatgc ccaggcgcgt gtgcttgtcg
    37861 ccgtagaaaa caaggcaacg gaaacggtct ttaatgcctc tggtattgac tttgacgcgg
    37921 tgaaggcttc tgccattgcc tatattcatg ccaatgtcat ggtacagaaa gaaaatagcg
    37981 gacagattga taagaaacta tctgagaaag agttaccaca catctaggag gaaatatgac
    38041 gaaaaaaatt gtagccttgg ctggtgatgg aatcggtcct gaaatcatgg aggctggcct
    38101 agcagtcctc gaggctgttg ctgggcaagt aggatttgat tatgagattg aggagagagc
    38161 ttttggtggc gctgggattg atgcggctgg gcatcctcta ccagatgcta ccttgcaagc
    38221 ctgccgtcag gcggatgcga ttttgttagc agctatcggt agccctcgat atgatgatgc
    38281 cgctgttcga ccagaacaag gtctgcttca acttaggaag gaattaggtc tctttgccaa
    38341 tatccgtccg gtgaaaattt ttgatagtct gaaagactat tctcctctga aagccgaccg
    38401 gctggacggt gtggatttgg tcatggtacg ggagttgaca ggcggaattt attttggaga
    38461 gcacattcta gaaacagacc gagccagcga tagcaatacc tatcaggcaa aagagattga
    38521 acgagttgtc cgctcggcct ttgacctagc ccaaaaaaga cgaaaaaaag tcaccagcat
    38581 tgataagcaa aatgtgctgg caacgtcaaa attatggcgg aaggtggcag atgaagtggc
    38641 gaaggactat ccagatgtga ccttggagca ccagttggtg gatagcgcag ccatgctttt
    38701 gattaccaat cctagtcgat ttgacgtctt ggtgacggaa aatcttttcg gagatatttt
    38761 gtcggatgag gccagcgtat taacaggaac gctaggggtt ctgccttctg ctagtcatgc
    38821 ggtagcaggt cccagtctct acgaacctat ccatggttcg gcaccggata ttgccggtca
    38881 gggcattgcc aatcctgtca gtatgattct atctgttgcc atgatgctgg aagaaagttt
    38941 tgatttggtc caagcaggtc agaccattcg ccaggcagtt gaagcggtct ttgcagaggg
    39001 cattttcact aaagatctag gtggtagcgc ttctaccaaa gaaatgacag cggctattat
    39061 tgaacagctt gaaaggaggt caccatgagt ggcaaatcca ttttcgataa gctctgggat
    39121 tgccatgtga ttacagggca ggaaggacag cctcagctcc tctatgttga tcaacattat
    39181 gtccacgagg tgaccagtcc ccaggccttt caaggactga gggatacagg gcgacctgtt
    39241 cggcgaccag acttgacctt tgcgactttt gaccacaacg taccaaccgt tgatattcac
    39301 aatatccgtg atgtcatttc caaggctcag atggatgcct tggctaagaa tatagaagat
    39361 ttcggcattc cccacgcagc ccacggttcg gaacatcagg ggattgtcca catggttgga
    39421 ccagaaacag gcaggacaca gccaggtaag ttcatcgtct gtggggatag tcatacagcc
    39481 acccacggag catttggagc tatcgccttt gggattggca cttccgaagt ggagcatgtc
    39541 ctggccaccc agaccatttg gcaggtcaaa cctaagcgac tcctagtgga atttgtcggt
    39601 caggcgcaaa gaggggtcta tgccaaggac tttatcctgg ctctgattgc ccgctacggc
    39661 gtggattgtg ggctgggtca tgtggtcgag ttcgcaggac ccgtcatcga ccgactcagc
    39721 atggaagagc ggatgactat ttgcaatatg tccattgagt tcggctctaa gatgggcatt
    39781 atggctccag accagaccac ttttgactac ctgcgtggtc gggagtgtgc tccagcggac
    39841 ttcgatgcag cggtggcgga ctggaaggag ctctactcgg atgcggacac ggtttatgac
    39901 aagcacctaa tccttgatgt gtctgagttg gcacccatgg tcacttgggg aacgacgcct
    39961 gctatggggg ttagctttga cgaaaccttc ccagtcatcc gtgaccacaa cgacgagcgg
    40021 gcctacgcct acatggattt ggctccaggc caaaaggctg cggacattcc cataggctat
    40081 gtcttcctgg gctcttgtac caatgctcgc ctcagcgatt tgcagctggc agctcggatt
    40141 gttaagggac ggaaaatagc agagcaggtg acggctatcg tggtaccagg cagtcgccct
    40201 gtcaagcgag cagcagaaaa gcttgggcta gacaagattt ttatggaggc tggttttgag
    40261 tggcgggatc ccggttgctc catgtgtctg ggtatgaatc cagacaaggt cccagcgggt
    40321 gtccactgcg cctctacaag taatcgaaac tttgaagacc gacagggctt tggggccaag
    40381 acccacttgt gtagtcctgc tatggctgcc gcagctgcgc tttatggacg atttgttgac
    40441 actagacagc tagatattct caaggaggga gtctaatgca ccaatttacc acatggacag
    40501 ggacgactgt tccgctcatg aatgataata ttgataccga ccagctcttg cccaagcagt
    40561 ttctcaagct gattgacaaa aagggcttcg gcaagtatct gctctatgct tggcggtact
    40621 tggatgataa ctacacggac aatcctgatt tcattctcaa tcagcctgag tatcaaggcg
    40681 ctagtatcct catatctggg gataacttcg gagcaggctc ttctagggaa cacgctgctt
    40741 gggctctggc agattatggc ttcaaggtta tcattgcagg ctccttcgga gacattcatt
    40801 acaacaatga cttaaacaat ggtattctgc ccattatcca gcccaaagaa gtcagggatc
    40861 aactagctca gctgggtcct gaccaagaaa tcacggttga cttggcagat cagttgatac
    40921 ggacgccttt ttgggaattc ccatttgaca tcgaacagga ctggaaacac aagttgctca
    40981 atggcttgga tgatattggt attaccttac agtatcagga tttgattgct gagtatgagg
    41041 ataatcgccc aagctactgg caaaactaga gtcttccatt ttatctttta ttacttcgat
    41101 gaacgaggaa actccgtttc cctatttcca acttcaaata atttcatcat tatttgaact
    41161 cgctttgccg tattttacaa gcgttactta ctatcgtaag tatgatttct aaacttcaac
    41221 agtcactact ctgactgttg aagctgtctg cagctcgttg acacgaacaa tattcgcttt
    41281 atctccaacc tccaactata tcccagatag ttggagcgag tactaaaagc aaactgaaag
    41341 actgactcct acgaaatatt agaaaacaag caggtcgctc tgctatgaaa caaaagaaaa
    41401 gaggaaatta ttatgacgaa acaaattcac tgggatggcg cactttcaca agaaggtttt
    41461 gacattatca agggtgaggg aggcgtgatt gtctgcccga ctaaggttgg ttatatcatc
    41521 atgactgctg acaaggctgg cttggagcgg aagtttgacg ctaaagagcg caagcgtaat
    41581 aagcctggcg ttgtcctttg tggtagcatg gaggagcttc gtgagttggc tcagttgact
    41641 ccggagattg atgccttcta ccaaaaacat tgggatgcgg acattttgtt gggttgtatc
    41701 ctgccgtgga aaccagaggc ttatgctaag ttgcaggctt atggcgatgg tcgtgaagag
    41761 ttgatgaccg acgttcgtgg tacttcttgc tttgttatca agtttggcgt ggctggcgag
    41821 cagattgcta aggaaatgtg ggaaaaagaa ggccgtatgg tctatgcgtc atctgctaac
    41881 ccgtcaggta agggaaatcg tggcaaggta gaaggtatcg gtgagcgaat tgcttctaag
    41941 gtggatttga tcatcgaggc ggatgactat gtggcttcta tccagccaga caagaccatc
    42001 gaaacccgct atgagcaagg ggttatggta tctatggtag acaaagatgg taaactgatt
    42061 ccagaacaag gtgcagatag ccgttctatc agcccatgcc cagttgttat ccgtaagggt
    42121 ttggacattg ataaaatcat gatgcacctg tctgaccatt tcaactcttg ggactataga
    42181 caaggggaat attattaaaa tataaagtag tacgatccga aagggtcgtt tttttgtact
    42241 cacataaaaa actgaccgaa attcagtcag tttggtgctg cttgatgaga aataggaggt
    42301 tatcgactag ccttttctcg tctgtcagtt ctaagatttg tagtcctttt tccaccagtt
    42361 cttgcttgtt ttgggctatg cctagataga cataggcacg ggctagttgg tcatagcgtt
    42421 tttctttctt ggctaggtcc atgagtttgg tggcaaaaca agtagacttg ctgtactgtc
    42481 tttgttccaa gaaaaaataa gaaagtgatc gataatgtga cagtatggtg ggttgaattt
    42541 tcttgtaatc cttgtatttt tccaatcgtt ttagaagaag attggcagtt tcttcaaggg
    42601 tatcaggtga agaaatcttg gacagaagag tgcctactaa gataatatcg ttatggtacc
    42661 agttatcgta tccttctagt tgggaccaga ggagtttgga cagggtttca gcctcgtcgg
    42721 aaagctgggt gatagaagaa ctttttcgaa tagctacaac ggtctccagg aggaggcact
    42781 ttctgtgaat aggaatatca ttgggttcat ttttcaaatg gtcctggcaa cgctttatca
    42841 aatcctccat ggtcttagtg gtcggatttc tgagattatc tatatcgtag agcaattgtt
    42901 ggcgttggct gggttgatag tagttacaga ggtattcaaa ctcctcaaaa ctcatatcca
    42961 cctgcttgag caagaaacgc atgagctcat agctaggaat ggtttgattt ttctcgaagc
    43021 gaaccaaagt agtccgattg agataatccc cacaaatctg ttcctgactt aaatgcttac
    43081 ttttccgaat actcttatat acacttccgt aatcccaccg cataacctac tccttgaaaa
    43141 atgtgattat ttgcaacaaa acgaattaac tttataaaaa tagtatatca taataatgcg
    43201 gaaatatttt tttgtatata gaaaataggt tgatgaaagg agtaacctaa tgaaacagat
    43261 tgataaaaac accttatttt taagtgcagt tattgttctt tcgtgtagta tcctattatt
    43321 tcatatggac ccgattcaga ttgtagatag agagatttgg ctctggctaa attcatttat
    43381 tcatgtgcta tttttaaaac ctgctgctag tctaggtttt cttatggggc tttatctgct
    43441 atacaaggct ctcaagaagt agtcgagcta actaagctga tgaaagtctg ctgcaccgct
    43501 ctgccatttc ccaaagaaag gagtttctat gctaaaagta gttaatgtct gcaagcgata
    43561 cggtgcccac caggtcttgt cctatgtgtc ctttgagttg caagcggggg acttggttgc
    43621 cctagtcggt cccaatggtg tggggaaatc cactctgttg aatatcatca gcaatacaga
    43681 aacggctgac cgtgggtcgg taaccatcaa tggtcgtccc aatagtgacc gagcgatttt
    43741 tcaagatatg tctgtcatgt tggacgccca agccctttat ccacagttga cgggttacga
    43801 ccatctgacc tatgtggctg ccacccacaa gctagggaaa agggaagtgg atgcgttggt
    43861 ggaagaattg gggatgggct actatgtcaa aaagcgggtg gctggctatt ccatgggcat
    43921 gaagcagaag ttgctctttg ccatggctgt ccttccaaag cccaagctct tgttgctgga
    43981 tgagccccat attggcttgg atccgaccaa tatcattcaa cagcgggaat ttcttctaaa
    44041 cctacaagca gaaggtgttg ccattttgct ttcttcccac catttgtcgg agattgagaa
    44101 gctgactaat caggtttatt tcctcaagtc cagcaagttg attgccacag aagtggagtt
    44161 gacggctgat tatgattacc atcttgctgt ggaaaactcc aagcaggttc aggaattttt
    44221 acgggattat cctcaactct tgtggaaaaa ggcagagcag gggctggtgg aaatcttctt
    44281 gcctgagaga gatttactag actgtctgga tcgtattcca atccagcagg tggctggcct
    44341 tacaaaagcc agcgactata tggagtccct ctaccgtgat ctctacgtgg aggaaggtgg
    44401 tgaggagata tgaggctgaa ctttgaaatc aagaaaatcc tctggtcatc tctctactgc
    44461 ttgctcgtgc taggttttct aggtctggcc taccttccca tctcacaacg tcaagcccac
    44521 caagcggcct atgtggaaac ctataaaaaa gaactgagtg aattgactag tgttttggca
    44581 gagataaaaa ttgaggaggg ctcagaagaa gcaccgcgga tgctgggctt gaaatccgat
    44641 atagaaagtg tcggtcaggc cttagatgag gggaagtttg aagctatccc caactatcgc
    44701 aaactcatgt atcaggattt ggactatttt atcagtcgca atcgacagct ctttcaaact
    44761 cggaattcct tgacacaaac ggaagtcgaa cgtttgacca gatggaatga ctggttgctt
    44821 gcctaccaat tgcctgatgg tcctgaaaac aaggcttggt caacagggcg tattgttcaa
    44881 gcctacttag aaagtttgtt tggttttctt cctgtatttt tggtgcttgg ttttctactt
    44941 gccaatcaac tgtcagaaaa gacacttgcg catcaagttt ggcaattgcg gcaatgggat
    45001 agtctgagtt tgcaggggat gaggcgtttg gttgcttact tgctttcact tttgttggct
    45061 gtggggatag gtttgctctt tatcctggtc tatgatgggc tgactggtca gttggctgta
    45121 tctagttttc aagctcctct tgagattttg ggtcagagtc agttgatagg ctcttggctt
    45181 tacgggctta tcttgcttgg tttttggcta atcttactcc tagctattcg cctacttttt
    45241 ggatttttgg accacttgtt agccagtcgg ctgacttttg tggtgattgg attactgttg
    45301 gcgttggttc tttcctatca ggctggtgca gtagctggtt ggtcaggtca aattctgcct
    45361 ttgttcgcct ttccgagttt cttagacaca gctgaatttt cacaggtctt tgtagcggtg
    45421 gatgttttcc tcctgctgac cattgggctg ttctttgctt attatgtgct gacagaaaag
    45481 aaaaggttgg tcttgtcagt tggtcatcag aaagaatggg aaaccggtcc tttggacaag
    45541 gtttggtctt ttgaaatact catccttagt cggctcaatt cttggaaagg gctcgtctta
    45601 gtaaacttgc tcttagctct gctattttcc ctactggcgg gactagaaaa gcaagaggtt
    45661 gcccaggagg agagggtttc gatggagttg gttgcccagt cctataagca ggatgggatc
    45721 tatgtggaga gtttgcagtc ggaaatcaac cgtctgaggc agttggtcaa agaggacgcg
    45781 ggctatcagg aggagttgga gctgaggctt ggtctcttgc aagaatatcg tgtctataag
    45841 gacctctatg tcaaacagtg gcaggtttat caggaaaaac ctcaggctct gccggctagt
    45901 caccttgcta tgctggaggc agagcaggtc tatctatggg ctagagagga gaaggctttt
    45961 gccaatggtg atattggggc cagccagacg gtagataatc ttcgccgtta ccagacccgc
    46021 aatcaggtca gtcagtatta ctggacgcag ctgatggagg ctggggtgcc agcaactaag
    46081 cggggcaagg atgtcatttt gactagcttg gaaaatcgtt ttgacacaaa tgatgccttg
    46141 gttccagtag acgatccctc ttgggaccgt tctagagatt tgtcaggtat cggtagcctg
    46201 cgctctcttt ttgatggtcc ggcctatttg ttggtgctct tgctggcggt ttggattggc
    46261 agtcggggca aggcggtgga agttgttgaa aaaaactggc gtctctatca gacccagcct
    46321 gtggatttgc gtcaggttct atttaccaaa tggttgctgg gactgggcca ggcacttggt
    46381 tttacgctgc tctttctagt tagtctattc ctagtcaata gtctggtcgg aggaatcggt
    46441 tatctggaag atggccttat cttgctttcg cattcggaga ggggtatttt tcttgaactc
    46501 ctgaggggac aagaactctg ggcttggttc ctgcccaatg ctccttatct cttgtggatg
    46561 gtgtttggct tgcttgtctt ggaaattgtt ctagtcagtc tcaaccatct tttgcaagtt
    46621 cggttttcaa accgtttagt ggttttcatc ctactagctg gtttgctggc tctgctggac
    46681 tacctcctct ttcaagacct gattttggat gggcaggaaa tttttaggca gttagctctg
    46741 ctattttcat aaaaaaagaa aaactggctg aaaataaatt cagtcagttt cttatgttta
    46801 ttcaatcacc aacatgcctt ctttaacggc aaatgggtgt tctgggtcaa tgcggtcgta
    46861 gaacatgaca ccgttgaggt ggtcgatttc gtgttggacc acgatggcat taaatccttt
    46921 gagtttaata cggtgtgttt ctcccttttt gtccatgtag tcaacagtca cgcgcgcatg
    46981 gcggactacg taaccttgga cttcgcggtc aacggataag cagccctcgc ccccttccat
    47041 agcagcttct tggacagaat gtgccacgat tttagggttg tacatgacct cttggaggga
    47101 gtaggcttgg gcaggtggat tgccttcttc gtcctctgga tttgggacta agacagcaat
    47161 gatgcgtttg gaaacgtcaa tctgaggtgc agctagacca actccgccac ggagtttcat
    47221 cttctccgcc atgacaggat cctgcgaatg tttgaggaat tgcatcattt tttcgcctaa
    47281 aataatttcc tgatcagata ggggaaatgc gacttcttca gcgacctggc gtagggttgg
    47341 atgcccttca cggatgatgt catccatgtc aatcaaatgg gcagcttttg tcaatcgttc
    47401 gattacagac atgattttct cctttgaata tagttaagat aagtatagca tgaaatggga
    47461 gagaataaaa gtcggacttt tctatccatt cgctttcaaa tctgttatac tagtaaaaga
    47521 cgaacgttga aagggagaaa aatgaaaaga attttagaaa aagccagttt gctggcccta
    47581 tccaccatgc tggtttcgac atttgctgtc tcgccagcta ttccacagat gattgagcat
    47641 tttgcccagc agggaattgc agcacctcag gtggaaaact taattacggt gacctctttt
    47701 gctattatgg ttactctatt ggcaaacggt ctagttgtcc gtttcttgtc tgaacgaaac
    47761 attatcattg cagggctttt gcttatggcc atcggtggag ctttgcccat gtttctaact
    47821 gcttatcctc tgattttatt ggctcgcatc ttgcttggtt ttgggattgg cctaatcaat
    47881 gcgcgtgcta ttaatatcat cggtaatttt ttcaccggtc aggagcgggt gcagatgatg
    47941 ggcttgcgtg gttcagcaga ggttttggga agtgcagggc ttaccctcct tgtcggttgg
    48001 ttgacccagt ttggttggca acctgccttc ttggtctacc tttttgcctt ggttatcttg
    48061 gctctcttcc ttctctttgt tcccaaggag gaattgctgg ctcgcatgga ggtaaaggtt
    48121 gaggagaacg cgcccaaggt caaactggac aagaccatgt ggatgatggg aatttatctg
    48181 gcattcttag cctcctttgt tatcaatgtc aataccttcc tgaccattcg tattcctcag
    48241 attgttttag ccaagggcat cggtactgct cagcaagcca gtctcattct cagtcttatg
    48301 caggtcatgg ggattgtggc agggacggtc tttagtagcc tggttggtcg tttgaagggt
    48361 tggcttttag ctgtttccta cgtggtcttt ggactcgcgg ttgtcggcat tgcctttgcg
    48421 gataatctct gggtgctggg actcggtggc atggtgtcag gatttttcta cagcatcatt
    48481 ctaaccattg tctttagtca ggtaacagac cgtgcaccca aggccttgct caatgcggtc
    48541 atgacaatcg tccttatggg ctgcaatatc ggcggtgcga catctgctat cttgccgact
    48601 tatctggaaa aactcaaccc aaccccaaca ggtgcctttg gtatttacgc catcggctgt
    48661 gccttgataa gcgcaggctt gatttataaa caactgaaga agtgggagtg agaaagaact
    48721 cgactggtct aaaaagagtt cgtcaacaag tccttgtttc cagttgttga gctgaaacag
    48781 tctatcccca gactgtttca ctcccacccc cgcacagctc aaactgtctg ggagacagtt
    48841 tgaggttgga gatgaagcga actctgagta gccgctgggc tttttgccca gcctcttttg
    48901 gtataatatt cttatgttag aaaataaccg cttaaatgaa ttaatactac gttttcccga
    48961 ggatgttcag ttgattttta aggacatgat tccctcctat cagttgggct atgtggatta
    49021 tgtctatcag acagataatt acgccaccca aaaccgtcgg attaaaaatt tagcaaaaaa
    49081 tttccgaaga atggattatt tttacatcat gcccctgccc caggatctgg cattggaaat
    49141 tgccaaccaa tggcgctatc aaccaccttt ggatgcctat acgattagtg gaaatcccga
    49201 gacctatgca gagatgattt caccagaagc gcgtgggaac cgcttttttg cagtcatccg
    49261 caatgccgcc ctcatgggct atttctgtat ggaccaagat ggcgagaccg ttgacattcg
    49321 tttgggcatg aaaccttctt tgacaggtca gggaaatggc agagctttct accagactat
    49381 tgaagactat ttagtagagc atgttcagcc agctagtatc aaactgacag ttgctagctc
    49441 tcatcaagtt gcccaaaaat tctttcctgc tcttggtttt acagaaatag aaaagcaagc
    49501 agaatatagc aaaatggaaa agaaagtcgt atgcgattag ataaattttt agtggattgc
    49561 ggtgttggca gtcggacaga agtcaagaaa ctcctgaaaa acaagcaagt cacagtcaac
    49621 ggccaagtag aaacctcccc aaaacagcag attgatgaaa agtcagatca gattgcacta
    49681 gcaggtcagg tcttgagtca tgaaaccttt gtttactatc tgttaaacaa gcccaagggt
    49741 gtgatttcag ccacggagga tgaccgtcat cggactgtct tggacctgct agatagtaca
    49801 gctcgtcaga aggaagtttt tcctgttgga cgcttggaca ttgataccca tggcttgctc
    49861 ctcttgacca acaacggtca gctggctcat gccatgcttt ctccgaaaaa acatgttgat
    49921 aaactctacc gtgcacaggt ggacgggatt atgacccaag aggatgtcga acgatttgcg
    49981 gcaggaattg agctgaaaga ctttacctgt caaccagccc agttgaccat cctctctaca
    50041 gatgaagcta agcagacctc gctcgttgac atcaccatcc gagaagggaa atttcaccag
    50101 gtcaaacgca tggtgcaggc ctgtggaaag acggtgacag acttgcaacg attgaccatg
    50161 gggcctattc gcttagatcc aaaattagcg cttggagaat accgcaggtt gactgctgcc
    50221 gaattaaagc aattagaagt ctttggtgtg gaattataga taaatagaag agacttgctc
    50281 ccgattggaa gcaagtcctt ttaaatatcc gcagctgcaa aaacaacctg tccatcttga
    50341 aatttttcaa tacgagcgag agaagtaaca gggacacctg catccagtaa gagctggcga
    50401 ccatcttgga aggacttttc gatgatgata ccaaccccag ccacttgcgc cccagcctgt
    50461 tggataatgt caatcaggcc ttttgctgct tgtccattgg ccaaaaagtc atcaacaatt
    50521 agaacacggt cctcaggtga caaaaactta ctagcaatag aaacggtgct agtaacttgc
    50581 ttggtaaatg aatagacttc ggcggtcaaa atgccttcgg tcatagtaat atttttatgc
    50641 tttttagcaa aaatcattgg cacatctagg ctttctgcaa catagacggc tggtgcaata
    50701 cccgaggctt cgatcgtgac aaccttggta atttctttag agcgataggc atctgccaag
    50761 acctgcccca tttctttcat caagtgaaaa tcaacctgat gggtcaagaa tgagtcaact
    50821 tttaagatgt tttctcctaa tacctgacca tcttttaaaa tgcgtgcctc caaagctttc
    50881 atgtttatct tctcctccaa atgatacttt tgtgtattat agcatttttt tgccaaaatc
    50941 aaaacaataa acgaattttt gtatttagat tttgtatata tagacaaaat taattgaaaa
    51001 atacgaacat tatgtgtttg ttggagtgaa atgtattgaa aatgattata ttttctgcta
    51061 gactaatacc attatttagg aataaagaag gagaaatcat gtctcagaaa ataacaaatg
    51121 aacattcttc ggatatgctt tacggaattg atgaacaacc accaaaaggg atggctgttt
    51181 tgcttgcttt tcagcatatt ttagcagctt ttgcaggaat catcgcagtt cctctagtcg
    51241 ttgcaagtgc cttaggattg tcagtcgaag atacttcaat tatagtatca gcctctatct
    51301 ttgttgctgg tattgccacc attttgcaat ctaaaggtgt tggcccagta ggttcacgtg
    51361 tctctggtat gatggggacg gactttacct ttgccaatcc agctatcagt gttggtagcc
    51421 agcttgggat tgctggtatc gtgggtgcga ccattgctgg ttcatttgta gagattgctc
    51481 tcagtcgttt tgtcaaaccc ttgatgcgct tctttccgcc attgattacg gggactgttg
    51541 tatccctcat cggtattacc ctcatgccag ttagtatgga ctgggcagct ggtggagctg
    51601 gcgcttcaga ctatgcttca gttgaaaata tcagtattgc ctttatcgta ttggtcttca
    51661 ctttggcatt gaatcactat ggaaaaggca tgctaaaaac agcttctgtc ttctttggga
    51721 tggtctttgg atatgtcctc tgtattctgc ttggaaaagt agatatgtct gctgttggag
    51781 aagcagcctg gtttgctctt cctaaaattt tccattacgg tgtaaaattt gacctatctt
    51841 ctattctagc ctttattcca gcctatgtcg tatctttgat tggtacggtg ggaattatga
    51901 tggccattgg agaagcctca aatcaaaaga ttccttctga gcgggcagca aacggtgtgt
    51961 tagcagatgg tgttggttcc ttgattgcag gtatttttgg tgctggtcca aatacagcct
    52021 tctcacaaaa cgttggtctc attactttga caaaagttgc tagtcgtcat gtcatgattt
    52081 tagcaggtat tatcttgacc ctacttggcg tctttccaaa attatctgcc ttgatttcca
    52141 tcatgccgca gcctgttctg ggtggtgttg ggattatcat gttcggtttg gttgctgccc
    52201 aagggattaa gactcttgca actgtaaaaa ttggtgaccg tgagctcttg attatttcca
    52261 ttgcctttgc acttggtatt ggagtgactg ttcgtccaga attattgagt catcttccat
    52321 cagctcttca aatggtcttc tcatcaggta tttcaacagg ggccctggct gccttggtat
    52381 taaatctggt gttaaaagag gaaaaataag agttatttta ttagtttgct tagatgaaaa
    52441 gagaaggtcg ggcaaaaagc ccaggaccac tactcagagt tcgtgtcaac atctcagcgc
    52501 agtggttgat tggcagattt gttcgtgttt tgcactccaa atctggccta atcaactgtg
    52561 cgggggtggg aaaacgaact ctttttatct tggtcgagtt ctttcccact cccctttttg
    52621 gggtaagtaa aaaatgctaa gggtgtacag ttgtcgtatc tgaaattata gcccctgctt
    52681 gaccgtactt ggtataacat attacaatat atataagaac ctgtgttcac agttcatcgc
    52741 ttccattgga gtgtttgaag gtggagataa ggagaggaga ttcctgacca attaggtggt
    52801 aggcgaaaac tgccacagga gttttttact cgtcgaagca ataaagtaaa actaaatggt
    52861 gatagagtca agtattcctt caaaaaattg ccttgatgag caaaaacgat gtcttgcata
    52921 agaacacttt gtttatgaat ataggttcta agatttgtcc aagagaggtt attatatgtt
    52981 gaaatatatc cgtgcccttc cccttgttat agtagcggga gcggttgttt actttactat
    53041 ttttatctgt attgataatg gtgattggac ctacgttgtg actagagcgc tagggcttaa
    53101 ggaagaacat gccaataatg tttctttgat tgtaattggt gcccaatcac tttttctaac
    53161 ttcggattgg ttttacaagc ggattcgtgc ctttctcaat cgtcgtttgg aaaaggccaa
    53221 agggcttaat cggcagattt atctgggcta taaggcaggg attgagtttt tgatggcgtt
    53281 acccaaacga actattttat tcacattcta tctactttta gtagttgctg aaagtgttgg
    53341 tctgattgaa aaagccaagg actatgctgc ggtaatcatc ttcataattg ctgttgaccg
    53401 tgtgaccaaa atctggccgg aggaaaagcg gaggatgatt gagtttagcc agaagattaa
    53461 gaaacggatt aagagtgtgg gataagtcaa gattaagtat tttaggaagg caggactgcc
    53521 ttcttttttg ttaaaaaacc aaacaaaatg caatgtatat attgcaaaac agaaaatata
    53581 gtgtataata taactataaa aatacgaaag gaggatggct atgatatgaa gaacatacga
    53641 cttaagctgg ctcgtgtaga aaaagatatg acccaagggg acttagcgga ggctatcgga
    53701 gtgacccgcc agacaatcgg tctgattgag gcggggaaat ataatcccag tcttagtctt
    53761 tgtctatcga tttgccactg tttagggaaa agtttagatc aattattttg ggaggaagca
    53821 actaatgaaa aaaattgtga ctgatgagcg tgttcgacaa gaagaaaacc aagtctttgc
    53881 ctgggtaggc agggccatga attttctttt gcccctctca tttctcatca agagactggt
    53941 tttaaaatgg ccgtttgata cctatgtttt tgaactgttt gccatgttgg tagtttcggt
    54001 ctatctcttt tacggttatt ggaggaaggg gcttgatatg gagcaagggc cggcttggaa
    54061 aggttacctt tatctagaag gcgtgatttt gggaaccacc ctcttggtgg cttggaccaa
    54121 ttaccagacc tacggtcatc attatactgg tatttgggat ggacattttt gggcagtcgt
    54181 ttttatattc tttatttcta tgacctgtct tgttctgcta ttgctgaaca tagtatcttg
    54241 ggtaaatagc taccgtcaga agcaggtgga aaaagagttg gaagaagaaa tggagtaata
    54301 atgagcagtt cagctccctg ctcagccatt ttcctaaaaa ataaaaattt acaaaattcc
    54361 ttgccaaaag taagggctta tgttacaata ccaaaagcgt aggtttttaa actcatcccg
    54421 tgaggtgaga aagacagcag gaaaatcaat taagggccta tgactatgta tatttagaaa
    54481 gtctgggtct ttttgtgtac ccagaatggg agatttatga gtacaaaagc caatcttttg
    54541 tttgatgtcc atgagaaacc agccccgctt caagtgattt tgttgagttt ccagcacgtg
    54601 ttcgctatgt ttggtgcgac gattttggta ccgctgattc tcggtttgcc cgtgtccgtt
    54661 gccctcttcg catcaggtgt cgggaccttg atttaccaag tggcgacaca gtttaaggtg
    54721 ccggtttacc taggctcttc tttcgcctac atctcagcta tggccctcgc cattaaggaa
    54781 atgggcggcg atgtgtcagc tgcgcagaca ggtattctct ttgtcggctt gatttatgtc
    54841 ttgattgctg cacttgttaa agtgattggg accaagtgga ttgataccct cttgccaccg
    54901 attgtcatcg gtccgatgat tatcgttatc ggtttgggtc ttgccaactc tgccgtgact
    54961 tcggcaggct ttgtcgcaga cggcgactgg agaaatgtcg tagcggctat tgctaccttc
    55021 ttgattgcgg cttttgtcaa taccaaaggg aaaggcttcg ccaagattgt tcctttcttg
    55081 attgccatca tcggtggtta tgtgattgct atcttcctcg gactagttga tttcacaccc
    55141 gttttggaag cagcttggtt tgaattgcca ggtttctacc taccatttga aacaggtact
    55201 ttcaaatcct ataatttcta ctttggtcca gaaatgattg ctatcttacc aattgctgtt
    55261 gtaacagtag ctgagcatat cggtgaccac accgttctca gccaaatctg cggccgccag
    55321 ttcttgaaag atccaggtct cagccgtacc ttgattggtg acggtgtggc gactgccgta
    55381 tcagccttta tcggtggacc tgctaatacc acctacggtg aaaatacagg ggttatcggt
    55441 atgactcgca ttgcatcagt atccgttatc cgtaatgcag ccttgattgc cattgccttc
    55501 tcgttcttgg gtaaatttac agcccttatc tcgactattc caagtgccgt acttggaggc
    55561 atgtctatct tgctttacgg tgttatagcc tcaaacggtt tgaaagtttt gattgagagc
    55621 cgtgttgatt tcggtcaagt ccgtaacctg attattgcaa gctctatgtt ggtattgggc
    55681 cttggtggag cagttctcaa catcggtgcc atcacccttt caggtacagc cctctcagcc
    55741 attgtaggta ttatcctcaa tcttatcttg ccaaaagcag aaaaggctga gtaaaattga
    55801 aaataacagc aagtcagccc cggctggctt tttttgatgg tgtagaggag attggggtga
    55861 aataattagt tttcgaggag tgtgaaaggt ttatactcaa ttaaaatcaa aaatagccga
    55921 tatccaatct ctatgtacaa ttgatttgag ttgataagcg gttaaggttt gaataatctc
    55981 ttgaagttta tcgattacct catttaaagt cttaaatact ttattcttga aaccaagttt
    56041 acgaatctca gcccaaactt gctcaatagg atgcatttca ggtgtatagg gtggaatgaa
    56101 ggtaaattct atattctcag gaatatttaa ggtctgagat ttgtgccaga tggcattatc
    56161 catgactaaa ataatataat cttctggata agctgctgat agctggcgta gaaattcatt
    56221 catccaatca ctattacatc cacctgctat gatgaaaaat gagtcacctg tatgggcatc
    56281 cacagctcca taacaatagc ggtactcccg aatatggtga ctagctacgt gagggcgata
    56341 accttttgga gaccaacacg aaccaagttt actgatacgc ccaaaacccg cctcatcttg
    56401 atacataagg cggatttttg atattctttt ccattaataa aacgcttact cactctctcc
    56461 ttgaataaag atttgatttt tagacgctaa aatggtttcg gcgtctgctt tttcggatgt
    56521 tggggacgtg ggctaacttt tgttgcttac tgcgtaagct ttatctccca cctcaaagaa
    56581 tctcttgatt ctttgaccca accatgacgt ttcaggagag catagaaacc ttctcgggtt
    56641 gtcttgtgcc caactctttc ttgataggct ttaaagaggg aggtaacggt aacgaattct
    56701 ccattcaaag aagaagagag ttgctcttta aggaaatctt cttcctcctc atgcgtcata
    56761 taggagcggc ggcaactgcc acgattttct tttaagaggg cttctaagcc accacttaga
    56821 tagcgattca ataagttgcg gactgtctga tggacaaagc ctactgattt ggctactttt
    56881 gttaaattat tgcattcaga ataaagaatg agtgcctgta tttttccatg atgcggagaa
    56941 ttatttttat ctttaagtgc ttgctttaat tcttcgactt gtgtttgggt aagttctttt
    57001 gtcataatac tattataacg ctatttttga tttttattaa gtataaatac tctgtttctg
    57061 atatgtatca tgtaaaaagt tttgg
//
LOCUS       DATUCG010000002        53783 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.002, whole genome shotgun sequence.
ACCESSION   DATUCG010000002 DATUCG010000000
VERSION     DATUCG010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 53783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53783
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.002"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(184..1146)
                     /locus_tag="VCA20_000052"
                     /pseudo
     CDS             complement(184..1146)
                     /locus_tag="VCA20_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735993.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
     gene            1284..2000
                     /locus_tag="VCA20_000053"
     CDS             1284..2000
                     /locus_tag="VCA20_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEP1833929.1"
                     /translation="MRLDKCLEKAKVGSRKQVKKLFKSQQIKINGQAAQSLSQIVDPE
                     LQTIQVSGKKVALEGSAYYLLHKPAGIVSAVTDQEHQTVIDLISPQDSREGLYPVGRL
                     DRDTEGLVLITNNGPLGYRMLHPSHHVDKVYYVEVNGCLAEDASKFFASGVTFLDGTR
                     CQPVDLTVLEASLDHSRATIKLAEGKFHQVKKMFLAYGVKVTYLKRISFGGFELGDLE
                     RGAYRQLTPNEMEHLFTYFD"
     gene            2014..2493
                     /locus_tag="VCA20_000054"
     CDS             2014..2493
                     /locus_tag="VCA20_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEP1833930.1"
                     /translation="MSIYDFTVQKQDGADQSMAEYQGQVLLIVNTAPGCGLAPQYKEL
                     QELYDSYRGQGFVVLDFPCNQFLNQAPGSAEDINQTCSLNYGTTFPRFAKIAVNGSEA
                     SPLYRYLKKEKSTLLGGRIEWNFTKFLVNRQGRVVKRYLPTTSPLKLKEDIELYLEK"
     gene            complement(2512..3513)
                     /locus_tag="VCA20_000055"
     CDS             complement(2512..3513)
                     /locus_tag="VCA20_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEP1833931.1"
                     /translation="MIRHHNYLKIFTFLLILLFVIFLLSLSIGYSNSSFSDLIDIMSG
                     RASSSTLLIIGRIRLPRIIASIIGGASLALAGLLLQTLTRNPLADSGILGINAGAGLV
                     VALFVGLSSKISPVLLSLMPLFAMMGGGLTIFLVYWIARKKLYGIHPARLIITGVGIS
                     SMLSGIMVSIISQLDDFKMEYIVQWLSGRVNGGDWKTIAIYTPLLLLVWMATFSRSRS
                     LNIMNLNDQTAMALGLHLQRERRTTLVLATALASLSVILVGNITFVGLIAGHIVRKWL
                     GNDHRIIIPATMILGSFILLLADTIGRVLLVGTGIPTGIIVSLIGAPYFLWLLKQEG"
     gene            complement(3510..4535)
                     /locus_tag="VCA20_000056"
     CDS             complement(3510..4535)
                     /locus_tag="VCA20_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEP1833932.1"
                     /translation="MNKLVSSRYPKTKPKNIWLVFFIICLSFIAGVYLSLRFGAISYS
                     HQQLIETLTHPLTDSSAQDVIIDLRLPRMVAAILVGAAMAQAGAMMQGITRNPIADPG
                     LLGINAGAGLALIVAYALFGGLHYSQILLICLLGSCLAAGLVFGLAYQVQKGYNQLRL
                     ILSGAMVASLFSAIGQAITIYFDLSTAVIGWQAGGLVQVNWKMLAIIAPLIIIGLILA
                     QLFSHQLTILSLNETVAKNLGQRTLLMTLVLLGIVLLLSASAVALVGSLSFIGLIIPH
                     FIRMFTGKNYKLLLPLTAFAGASFLIWVDLVCRSINPPVETPISAVISIIGLPCFLWL
                     IRKEKHF"
     gene            complement(4549..5478)
                     /locus_tag="VCA20_000057"
     CDS             complement(4549..5478)
                     /locus_tag="VCA20_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEP1833933.1"
                     /translation="MKKFLAIFSLFVGLVFLTACSTSSSSTDVELSSMPKIEGITYHG
                     DIPKNPKKVVNFAYSYTGYLLQLGIDVSSYSLDLEKNSPVFGDKLKDVPQLTTADTEA
                     IAAQKPDVILVFSGDDNLETLKEIAPVIEITYGKSDYLKMLTDIGQIFGKEKEAQAWL
                     DQWDKKVATAKEELKGLIDTSSTFTVMDFFDKNIFLYGNNFGRGGELIYRALGFAAPA
                     KVQEDIINKEGWFGVSQEALPEYVGDYVLVNVNDKTKEAAASLKESDIWKNLPAVKNG
                     HVIEIDYNLFYFSDPMSLDLQIDAFVSEIQKLQ"
     gene            complement(5491..6276)
                     /locus_tag="VCA20_000058"
     CDS             complement(5491..6276)
                     /locus_tag="VCA20_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019774490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1833934.1"
                     /translation="MSSIQAENIQVSYDNRIIIDELSTSIPKGNITTIIGANGCGKST
                     LLKALTRIIPVQEGAIYLDGQAISQLPTKEVAKKLALLPQVLEATEGISVYELVSYGR
                     FPHQNGLGHLTDQDREKINWALEVTQTAPYARLPVDDLSGGQRQRVWIAMALAQDTDT
                     IFLDEPTTYLDLNHQLEVLELLKELNQSRQKTIVMVLHDVNLSARFSDYMIAMKEGDI
                     RYHGSVSNMMTTEILRDIFSIEPQLMQVAGQDYPILLTYDLKK"
     gene            complement(6428..7873)
                     /locus_tag="VCA20_000059"
     CDS             complement(6428..7873)
                     /locus_tag="VCA20_000059"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013990079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEP1833935.1"
                     /translation="MITIERVLEILKADANFCHIKQNDQTDSTWTDVQFDALSYDSRT
                     VSPRTLFFAKGLAFKKEFLEKAIEVGLAFYVSEIDYEVGIPAIIVHDIKQAMSLIAME
                     FHGHPQKQLKLLAFTGTKGKTTAAYFAFNILKQSKKPAMLSTMNTTLDGKTFFKSTLT
                     TPESLDLFAMMAEAVKNGMTHLIMEVSSQAYLVKRVYGLTFDVGVFLNISPDHIGPIE
                     HPTFEDYFYHKRLLMDNSRAVIVNAGMDHFEVVKEQVSSKDHDFYGPTSENQISQSAG
                     FDFTATGKLAGHYDIQLIGGFNQENAIAAGLACLRLGASLEDIHTGIAQTNVPGRMEV
                     LTQQNGAKVFVDYAHNGDSVKKLIDVVLEHQTGKVFLILGAPGNKGESRRKDFGLLLN
                     DYPQVEVILTADDPNREDPAEIAQQIRSHMTRPSDFILDREEAIRTAMSQTTSSRDAV
                     IIAGKGADAYQIVNGEKVAYDGDLEVAKQYL"
     gene            8019..8765
                     /locus_tag="VCA20_000060"
     CDS             8019..8765
                     /locus_tag="VCA20_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEP1833936.1"
                     /translation="MFYSYLRTLLTFLLWAINGNIHYHDREKILPQEENYILIAPHKT
                     FWDPVFLGYAAAPKQFIFMAKKELFKDRGFGWWISKCGAFPIDRDNPGMAAIKYPVNM
                     LKKSDRSLVMFPSGSRHSSELKGGVAVIAKSAKVKLMPATYVGPMTIKGLLAGERIDV
                     AFGNPIDVSDIKRMDDAGTAEVTSRIEAEFKRLDEHATSFQTKKKPNILTYIYRIPVL
                     LLVALILGLTYAFSYIASFFWQPSTQLDKK"
     gene            8832..9494
                     /locus_tag="VCA20_000061"
     CDS             8832..9494
                     /locus_tag="VCA20_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEP1833937.1"
                     /translation="METIKTYIEMLKEYKWQIALPAVAGLLMATFLIFSQPAKSDQTG
                     LTDFPQTAQTSSSSNLVEETSTEVSEEPSQLVVDVKGAVAKPGLYTLEADARVNDAVE
                     AAGGLTSQADPKSINLAQKLSDEAVVYVASKDENISVVASTTTSSAMSQEEKNTSLVN
                     LNTATEADLQTISGIGAKRATDIIAYREANGGFKSVDDLNNVSGIGDKTMESIRPYVT
                     VE"
     gene            9478..11715
                     /locus_tag="VCA20_000062"
     CDS             9478..11715
                     /locus_tag="VCA20_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEP1833938.1"
                     /translation="MSRLSKLPCQPIHLAVLAVAAYFAVHSFSLLTMGLFSLLLAVFG
                     VRQGKMVFLKTLPLLALCGLFFGYQKVQWERAAQSATEQVITVQVIPDTIEVNGDSLS
                     FRGRADGQTYQVFYKLASQEEQAYFQKLADLVQLEVEAEVSLPAAQRNFKGFDYQAYL
                     KTQGIYRTVKLTAIKKIVPVQSWNVFDWLSTWRRQALVYVKTNFPAPMSHYMTGLLFG
                     ELDSDFDQMSDLYSSLGIIHLFALSGMQVGFFIDKFRWILLRLGLTKETVDKLQIPFS
                     LVYAGLTGFSVSVVRSLVQKILGNLGLRKLDNFAVTVFICLLILPGFLLTAGGVLTFT
                     YAFLLTVFDFEDLGQVKKAAVESISISLGILPILMTYFYAFQPLSILLTFVFSFVFDV
                     LLLPGLSVIFLLSPVVKMTWVNGFFVFMEKIIVWVADLGLRPWILGKPSELVLLLLLV
                     SLFLLYDFHRRKKWLLGLSLVLALLFFITKHPLENEVTVLDIGQGDSIFLRDMRGRTV
                     LIDVGGRVDFAAKEAWQERSRQANAERTLIPYLHSRGVDRIDSLVLTHTDTDHVGDVL
                     EVAKQVQIGRIVVSPGSLTVPDFVATLKEIDVPVHVAKVGDRLPIFDAYLEVLYPDGT
                     GDGGNNDSIVLYGRLLETNFLFTGDLEQGELDLIETYPNLPVEVLKAGHHGSKGSSYP
                     EFLDHIEAKIALVSAGENNRYKHPHQETLERFDSRNIQVYRTDQQGAIRFRGWREWRI
                     ETVRE"
     gene            11811..12842
                     /locus_tag="VCA20_000063"
     CDS             11811..12842
                     /locus_tag="VCA20_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEP1833939.1"
                     /translation="MTVIEQIEKLKKEKLELLTVLCGEDIGQYQIAKDLLLRKLDFDA
                     ADLGFAYFDMSEADYHQVDLDLVSLPFFSDEKVVILDYFADLTTDKKRHLTDDELKQF
                     EAYLENPVETTRLVILAPGKLDSKRRLVKLLKRDGLVLEANPLKEMDLKNHFQKEIMR
                     LGLQMGGEVFQYLLEKSNFDFAEISKNLAFLQSYMGKETIEISDIDAAIPKTLQDNIF
                     DLTQLILQSKVDEARNLVRDLRLQGEEEVKLIAIMLTQFRTYLQVQILVEQGRGEQQI
                     VAELSTIMGRKVNPYQVKYALRDSRHLSIGFLKKVVKLLIDTDFQIKTGQFDKDYLFD
                     LLLLKIATT"
     gene            12955..13560
                     /locus_tag="VCA20_000064"
     CDS             12955..13560
                     /locus_tag="VCA20_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEP1833940.1"
                     /translation="MAIILPDLPYAYDALEPHIDAETMTLHHDKHHATYVANANAALE
                     KHPEIGEDLVALLSDVEQIPADIRQALINNGGGHLNHALFWELLSPEKTEISAELATD
                     IDATFGSFDAFKEAFTAAATTRFGSGWAFLVVNKEGKLEVISIANQDTPIMQGLKPIL
                     ALDVWEHAYYLNYRNVRPNYIKAFFEVINWDKVNELYKAAK"
     gene            13822..15846
                     /locus_tag="VCA20_000065"
     CDS             13822..15846
                     /locus_tag="VCA20_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HEP1833941.1"
                     /translation="MKKNIRLKSSILALVAGFSVIATQAVLADELAVQIMGVNDFHGA
                     LDMTGTARLEGETVRNAGTAALLDAYMDDSQAEFEETAAETETPAESIRVQAGDMVGA
                     SPSNSGLLQDEPTVKVFNKMDVEYGTLGNHEFDEGLDEYNRIMTGEAPKEGQFNEIVD
                     NYTHEAAKQEIVIANVIDKETGEVPYGWKPYAIKTIPVNDKEANIGFIGVVTTEIPNL
                     VLKKNYEQYTFLNEAETIARYARELAEKGVNAIVVLAHVPATSKDGVAAGEAADMIAK
                     LNEIYAEHSVDLVFAGHNHVYTNGTTGKTLIVQATSQGKAYADVRAVYDTDIADFKAV
                     PTAKIIAVAPGQKTPSPEIQAIVDEANTIVKKVTEQKIATASQATDISREVNEFKESA
                     VGNLVTSAQLAIAKKSGYDVDFAMTNDGGIRADLKVQEDGTVTWGAAQAVQPFGNILQ
                     VVQMTGEQIYTALNQQYDEGEKYFLQMSGIKYIYTKADNPTEENPYKVVKAFKEDGTE
                     IVPTETYTLVINDFLFGGGDGFSIFKEAKLIGAINPDTEVFVEYLTDLEKAGQTISAT
                     IPGRKAFVEKYVEEPKAEEKEDNAGTTTDVKTPEKANDGGDSVTNQKANEQPAPSGSM
                     APISNKKTEKASGNQTLPNTGQEALGSLLISLGGLVSLGMAVSMRRKEGE"
     gene            16041..16112
                     /locus_tag="VCA20_000066"
     tRNA            16041..16112
                     /locus_tag="VCA20_000066"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16074..16076,aa:Arg,seq:cct)
     gene            complement(16150..17244)
                     /locus_tag="VCA20_000067"
     CDS             complement(16150..17244)
                     /locus_tag="VCA20_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEP1833942.1"
                     /translation="MAYFRKRDNGWEYRISYKAPDGSYKQKSKSGYRTKAEAVQAASQ
                     AEIELSNGIVEDKNITLAEYFEKWMLIHKKPHVGPETFGKYEYTLKLITRYFHETKLS
                     KINATSYQNIINELAKCYVKDSVKRFNSHIRAAIKVAIHQGILKKDFTEIVKVFSDVE
                     SKKEEDKYLELDEYEQLITDYRKTIKYQSHFFLYTIGKTGLRFSEAAGITESIVDREN
                     MCLRIRRTYKVYGKKKGWGPTKNPQSERDVPFDSEWLKAYDEYMKVGYIDNPDKRLFT
                     KLTGTGENKILKKKTRQTFNVHGLRHTYVSWLIYHDVDVVTIAKLVGHKDATETLKTY
                     SHLFKAKQEESFDKVRNLMEKFGANLGQES"
     gene            complement(17369..17803)
                     /locus_tag="VCA20_000068"
     CDS             complement(17369..17803)
                     /locus_tag="VCA20_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEP1833943.1"
                     /translation="MAFQQGEVYLVNFTQKGGNEFYGKHYAIILTSPDKTDGTLLVVP
                     LTGKKAGKKYRGGITLDNTKYQDTPSKPKAYAYVRKIQEIDKRKIIYKTKKQVDSSGV
                     PLTDKSGKALFQKIYKPAYQLDQTDLDKLKAKIKEVLQLDIE"
     gene            complement(18038..18814)
                     /locus_tag="VCA20_000069"
     CDS             complement(18038..18814)
                     /locus_tag="VCA20_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1828 domain-containing protein"
                     /protein_id="HEP1833944.1"
                     /translation="MKANDIKKEYLAFVKENAVFTDIGEGHTEIVTPFVDPFGEAIGF
                     SIKANGRTFTITDESYTVWNLSINGIDVTKKGRRQELFKSLLQYNGFDLVGEAIERNV
                     TRTHLGQAVHDMTQLLINVYDFIQLSPNNVKSQFLDDVKHYFMHNEKYTVFPAFSIAG
                     KSRLEHRFNFVFMSKGYSKIARVHNNITKQQVDTILASWLDTVEYRKMEYGDKEQLYI
                     IVSDEGFNNIKDDHLMALQEYGISVLNFADKEQLQLQLGA"
     gene            complement(18840..19310)
                     /locus_tag="VCA20_000070"
     CDS             complement(18840..19310)
                     /locus_tag="VCA20_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1833945.1"
                     /translation="MKELLLTDAEAQKLIKILKVVAQKHNKTLHNQTNGKIDIVGKGD
                     KRFILNYFYSTNSKVFHIRETEHNYTLIRINLNNKFHKNANGKKVWGNRINIFSSEEY
                     YQKGDETTHYKAHPLPYESIRNTDDFLTALSDLLGFTNISNPEEININIQEDLL"
     gene            complement(19382..20227)
                     /gene="dinD"
                     /locus_tag="VCA20_000071"
     CDS             complement(19382..20227)
                     /gene="dinD"
                     /locus_tag="VCA20_000071"
                     /inference="COORDINATES: protein motif:HMM:NF008573.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HEP1833946.1"
                     /translation="MEQSKIYRTKEKFDSIVNQTENEFIDYWYARDLMPLLGYERWEN
                     FHKAIQRAMNSVETSDTKVSDHFRGVTKMVPLGSGSERPVKDYMLTRYACYLIAINGD
                     TNKEEIAFAQSYFAVQTRKQELIEERLHYIERTEARGKLKESEKRLSQNIYERGVDDK
                     GFGRIRSKGDTALFGGHSTQEMKERLGVKSNRPLADFLPTLTIAAKNLATEMTNYNVE
                     ENNLHGEKSITDEHVLNNTTIRNMLGQRGIKPEELPPAEDLKKLERKVKQQNKNLIKE
                     AGKLP"
     gene            complement(20240..20980)
                     /locus_tag="VCA20_000072"
     CDS             complement(20240..20980)
                     /locus_tag="VCA20_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP1833947.1"
                     /translation="MELNVYIGQKIKDFRKLAGMTQTDLAQRLETTKQTISRYEKGDR
                     KPGQDTLFELTDIFKVSIDDFFPPITTTAPNSLIEQISDKVVQLTEPNQKNVLRYSSE
                     LLDKQNTVTYSKNTVNELQATYYTYNYYDQPASAGTGQYLNDVQVEQVELPIEVNADF
                     VVQIYGDSMEPEYHSGDYVFVKLSVDLSDGDIGVFAYNGEAYIKQLRITDQGAYLRSL
                     NPDYDNIPITADTDFRTIGEVVDVYRER"
     gene            21152..21292
                     /locus_tag="VCA20_000073"
     CDS             21152..21292
                     /locus_tag="VCA20_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1833948.1"
                     /translation="MMDILKSLTPDELGTVLIVLGLVRQARLWHKQVLDHKIEQAKLN
                     KK"
     gene            21477..21683
                     /locus_tag="VCA20_000074"
     CDS             21477..21683
                     /locus_tag="VCA20_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP1833949.1"
                     /translation="MQTLLYGLRKERNLTQEEMADKIGISENAYRQKEKGQRPFTQDE
                     MFFLGKFFNKPIQEIFLPRESPKR"
     gene            21863..22189
                     /locus_tag="VCA20_000075"
     CDS             21863..22189
                     /locus_tag="VCA20_000075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1833950.1"
                     /translation="MPASGAVDGGLCAVGAGGEVERGVGRYGSAIYRKENDMNKKGRP
                     AGVKNERGLYTIAIPKEIYDQIDDLAIGSGRSRTAVATFVFTKGLEHIQIVEETITRK
                     RIVGIE"
     gene            22223..22381
                     /locus_tag="VCA20_000076"
     CDS             22223..22381
                     /locus_tag="VCA20_000076"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1833951.1"
                     /translation="MNLQILITLLLSTIFSLISFIVVTTAVDRKSKKMRDEMYKSVWD
                     EYKKRSGK"
     gene            complement(22383..22814)
                     /locus_tag="VCA20_000077"
     CDS             complement(22383..22814)
                     /locus_tag="VCA20_000077"
                     /inference="COORDINATES: protein motif:HMM:NF023890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1833952.1"
                     /translation="MENFQFWLTFGTATIPAAITGLLSFGASWMKNRSDIKAIKIQFE
                     HEMEKLSLSHKHELENIERQHSQELDALKQSHDLRLLELEKVSKLDAETDLALKQNEL
                     IYKVMTGEVNLNSVFSSADKVENYNQKQNLKKQFVQKLSKK"
     gene            22880..23590
                     /locus_tag="VCA20_000078"
     CDS             22880..23590
                     /locus_tag="VCA20_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HEP1833953.1"
                     /translation="MNEIININVNDNQEPVVSGRQLHEALGVKTAYKDWFPRMTEYGF
                     VEGQDFCSNLSESTGGRRAVDHIIKLDMAKEIAMIQRTDRGKQVRQYFIQIEKDFNSP
                     EKIMARALLLADKKVHQLEAQIEADKPKVLFADAVSASHSSILVGDLAKLISQNGFKI
                     GANRLFAWLRENGYLIKRKGSDWNMPTQKSMELGLFEIKETTITHADGHISISKTVKV
                     TGKGQQYFINKFLADDVA"
     gene            23674..23949
                     /locus_tag="VCA20_000079"
     CDS             23674..23949
                     /locus_tag="VCA20_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEP1833954.1"
                     /translation="MDDIAESLISRFISQLKVRLVEVFEVFNLELAMPLLLNSKQCKK
                     LLGIANETEFQRVSHLKDFPRIEKKGSHPRFPRDAVVEWMRVNWKLI"
     gene            23946..24215
                     /locus_tag="VCA20_000080"
     CDS             23946..24215
                     /locus_tag="VCA20_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1833955.1"
                     /translation="MTEAIFTLGIFAVPILTAAVVEQRKAEKERKRRELALLREMELE
                     TLKYETWAQAEQYYKTCQKESARNNLKRNGQQVDKEWKRHAEMVG"
     gene            24196..24390
                     /locus_tag="VCA20_000081"
     CDS             24196..24390
                     /locus_tag="VCA20_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1833956.1"
                     /translation="MQKWLDKLFKQEKPAIPRPLYTLEQENQILHDMVREIAEQRNEY
                     RIENQRLRDENAMLRRILEG"
     gene            24565..24930
                     /locus_tag="VCA20_000082"
     CDS             24565..24930
                     /locus_tag="VCA20_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001163840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEP1833957.1"
                     /translation="MQHIARNTHENYSKINNHPAQNSELSLQAKGLLFVLMSNKDTWR
                     PYIDQLSKRSKNGREAHRNAFEELKDGGYIRIYRKSLGRGRGIQNYPLVSDIPITDSY
                     WEYWKEKVDDELSTGESSE"
     gene            25296..25445
                     /locus_tag="VCA20_000083"
     CDS             25296..25445
                     /locus_tag="VCA20_000083"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1833958.1"
                     /translation="MLHRRYGMEGTYPWFDYDRDYLQPEEPRQVHDPDEWVFRGGQWI
                     YVGDA"
     gene            25445..25624
                     /locus_tag="VCA20_000084"
     CDS             25445..25624
                     /locus_tag="VCA20_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1833959.1"
                     /translation="MTEELLDTIRRLRCDYFHLGRELGEIINEQQDLILALKRENKRL
                     KRENWNLKQTKRRKK"
     gene            25621..26532
                     /locus_tag="VCA20_000085"
     CDS             25621..26532
                     /locus_tag="VCA20_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HEP1833960.1"
                     /translation="MSNLAVIQKDITDAVNAKVSQMQNEGLVVAPNYAPANALKSAFF
                     AMTNSPSGNLLEKCSKESIANALLDMVVQGLSPAKTQCYFIPYGSTLKMTRSYFGTMK
                     VVKQLSNVKDIWAEVVFEGDVLKIRNDNGRKVLESHETDWTNQDNAIIGAYCIIEKVD
                     GERILTVMTKKEIDRSWQQSKNKSVQNAFPQEMAKRTVINRAAKQFFNTSDDSDILIE
                     AVNRTTENEFDDNRQIKEAEPVQSAGQDILDKMTGKTVAEEPVEDATISEVETVEEAG
                     VDISKMETTEQVIDAEIGEILDEEEPF"
     gene            26532..27746
                     /locus_tag="VCA20_000086"
     CDS             26532..27746
                     /locus_tag="VCA20_000086"
                     /inference="COORDINATES: protein motif:HMM:NF018751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1351 domain-containing protein"
                     /protein_id="HEP1833961.1"
                     /translation="MSEALSLFDNLESMAPVPTATVLDFDFEFTPAQITIVGKDLLEQ
                     ALTGYVEKYKNYTVTAETFEDDAKVRAELNTLQKKVKSAVKEKLADYNKPIDEVKAWV
                     DGLLEPIVKIGKSIDEGVKAFEEQERLKRAKTIEELFQEVIASTGKDIDIRLFSKYFD
                     EFSKKTCFMADNVRPNKATVNMVASLVEEEVTKKEEYESALIKITEAAAKADFGPAPY
                     VRNFEKGASLADILQAIADDKALADRTREEVRRKQQLAKRIEEMTAIAESKGLDPKKY
                     ADMLESGVSALAVHEELVNDARKQQEEQDRMEQEFLAQHGAVCGNVQNRPNSDEIQRE
                     NVSEGKYTSEQKNASEDKIELNKKAVKLQGDFRITFPDGETAKLFGGKGGLYEQHGIV
                     VEKLGEWTKING"
     gene            27739..28575
                     /locus_tag="VCA20_000087"
     CDS             27739..28575
                     /locus_tag="VCA20_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease-like domain-containing
                     protein"
                     /protein_id="HEP1833962.1"
                     /translation="MAKLTEENYYQDRQWLSNSRFKAYMDCEAKAKAIDDKEWADNRD
                     DTALLVGNYVHTYFESEEAHANFVDANKTRMISSRGATKGELKKEFQVAQNMIDALKD
                     DKDFLPLYHGNPGDDVRKEMILEGEIFGIKVKGKVDSINLTEGYFVDLKTMKTICGLE
                     WSDVERKKIHGAAANILGFRYDVQLGLYQELLRQMGYPNFVPFVVAVSKEDVPDKAVI
                     TLSQYRLDEGLQFFENNVEHVAGIIAGEIKPKGCGNCDYCRSKRTLDRAINLDDLIAG
                     VF"
     gene            28791..28961
                     /locus_tag="VCA20_000088"
     CDS             28791..28961
                     /locus_tag="VCA20_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001868080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HEP1833963.1"
                     /translation="MTDNRKTVGRPKAEKPQDKRVTVRMTQETFEKFESYCETQKVTK
                     SEAIRRAVELLK"
     gene            29093..29326
                     /locus_tag="VCA20_000089"
     CDS             29093..29326
                     /locus_tag="VCA20_000089"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1833964.1"
                     /translation="MAKNQELINHIHLAFKEKGQSEQLMQWDNLISIVINRDFDAQKA
                     NTFFEAHACLLSVFANDLIEFVLDMFTEDVEDV"
     gene            29319..29861
                     /locus_tag="VCA20_000090"
     CDS             29319..29861
                     /locus_tag="VCA20_000090"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019096.1,HMM:NF019106.1,HMM:NF024784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEP1833965.1"
                     /translation="MSKEIWKDVIGYEGSYQVSNLGRVKSVSREIINRGVRQKIKERI
                     LKPCNKGGYLFVTLSQFGKLKTFAVHRLVAITFLPNPENKPQVNHLDENPLNNRLTNL
                     AWATANENCNYGNRNKRVFKVGVVKPRSVSMYSIEGELLHIFSSIAEASNFLGKSPSC
                     ISACAGGKRKKAHGYIWKYT"
     gene            30001..30180
                     /locus_tag="VCA20_000091"
     CDS             30001..30180
                     /locus_tag="VCA20_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018165975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1833966.1"
                     /translation="MIAHEKEYALYKGDKLLAIGTAKELAEQFGVKVSTIHFYKSPAY
                     IKRTNETKARRLVEV"
     gene            30170..30562
                     /locus_tag="VCA20_000092"
     CDS             30170..30562
                     /locus_tag="VCA20_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1833967.1"
                     /translation="MKFEFILSNTKRKKEMLNANDRMNWAQKAKITTYLRRIAFFKVS
                     EGKYTTHTKKRPCGLLVTVYAPTKRRMDPPNFYPTVKALVDGMTDAGIWTDDNHKVIK
                     KLSFVYGGLSKEKGHYKFIFDIDGVKDD"
     gene            30555..30893
                     /locus_tag="VCA20_000093"
     CDS             30555..30893
                     /locus_tag="VCA20_000093"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1833968.1"
                     /translation="MISGFSLICKKTRQVRSIQGALMFDTPHDAQKALKKDCKRCGEN
                     YDKELERYDIVDFMATQFEAGVLVNGMRYSLHRMKLSKEHLKSLSDGEKLMFSNHDIK
                     NLITLEVKDG"
     gene            30886..31122
                     /locus_tag="VCA20_000094"
     CDS             30886..31122
                     /locus_tag="VCA20_000094"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1833969.1"
                     /translation="MVNVLLDVTGFDKDKDKAFKLSLNVEKIIAIAEDTFVVFDDVAG
                     EYVEHVGCEITVDGSLCYKILEPYQKVKDKFVRC"
     gene            31123..31503
                     /locus_tag="VCA20_000095"
     CDS             31123..31503
                     /locus_tag="VCA20_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1833970.1"
                     /translation="MTKKKIERLSVIHRREITWLKWYFLRDKKNPKKTVLEQMIHDSF
                     MKNDTDQATFLVNLKLVTSEHVENSEEKLIETIKEVYVYENINVIGACQKILFLSPSP
                     AYTHLNKWFDAYFYATYKYLPLER"
     gene            31572..32243
                     /locus_tag="VCA20_000096"
     CDS             31572..32243
                     /locus_tag="VCA20_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4417 domain-containing protein"
                     /protein_id="HEP1833971.1"
                     /translation="MDILKPHHRQNTLSQYNLLDYDATRTAGKYNIPTLEPVDHVPTK
                     LQGFNYVLNKPNYSAGVHFFLDDYQFERVWKRPDFYIDKLADFDCVLTPDFSLYTDMP
                     LAMQVWNVYRSRLIGQMMQNWGYTVIPTVSWSHKDSYEFCFDGIPTKSIVAVSTIGIK
                     KSKDRIKVWKDGMDAMIDKLKPKKILVYGGHIDYDYNGIEVYYFANDTTERMDTWEAE
                     GQALE"
     gene            32213..32623
                     /locus_tag="VCA20_000097"
     CDS             32213..32623
                     /locus_tag="VCA20_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1833972.1"
                     /translation="MGGRGASSGMSEKQKKYGTEYSTVHQSGNIKYVTQNASGSQKTP
                     METMTKGRVYVLIDKNKNAPKSIVYFDKDNKRSKQVDLDHKHQKMRPHTHHGYNHAEY
                     EISKKGASKLTTTEAKLVEKVNTEWYNYLKKRRE"
     gene            32812..33291
                     /locus_tag="VCA20_000098"
     CDS             32812..33291
                     /locus_tag="VCA20_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HEP1833973.1"
                     /translation="MNDKQIRFADEFIKLGNATQAALNAGYSEKTSYSQGQRLLKNVE
                     VKEYINKQMQELHKSNIMQAEEALSILSDIARGKRDEEVLILNPTTGKVERHSKKTDN
                     ATVIKAITEILKRYPTAKQAEKLELEIEKLKSQLTEVQIEDETITIADKWEVEDESS"
     gene            33278..34591
                     /locus_tag="VCA20_000099"
     CDS             33278..34591
                     /locus_tag="VCA20_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HEP1833974.1"
                     /translation="MKVVNIQNNVNPHFKSVWVSKKPYNILKGGRNSFKSSVIALKLV
                     YMMVRYIIRGEQANVVVIRKVANTIRDSVFNKIQWALTLFGLMGRFRATVSPFKITHK
                     KTGSTFYFYGQDDFQKLKSNDIGKIIAVWYEEAAEFGSQEDFDQSNVTFMRQKHPLAP
                     FVQFFWSYNPPRNPYNWINEWFEGMKLQDDYLCHSSTYLDDVLGFVTPQMLADIERIK
                     ENDYDYYRYLYLGEAVGLGNNVYNMSTFHPLDALPSDDRLIGISFALDGGHQQSATAC
                     CAFGITAKGKVILLDTWYYSPAGQAVKKAPSQLSQDINSFIQSVISQYKVPILQYTID
                     SAEGALRNQMYLDFAIRWHPVAKLKKVTMIDTFQSLLAEGRFYYLNTENNKVFIEEHK
                     MYRWDDKTIQTDNPNVIKDDDHTCDVAQYFVLDNAKILGLRVGNS"
     gene            34603..36192
                     /locus_tag="VCA20_000100"
     CDS             34603..36192
                     /locus_tag="VCA20_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEP1833975.1"
                     /translation="MSLIQKVKDFFNRGRYNMTTSNLNSILDHPKVAVTQSEFRRIQH
                     NLAYYQSKFDDIEYTNTDGDRKRRKMQHLPIARTAAKKIASLVYNEQAEISAKDTTLN
                     GFLQDMLSNDRFNKNFERYLESALALGGLAMRPYVDGDKIRVAFIQAPVFLPLQSNTQ
                     DVSSAAILTKTIKTENRKNVYYTLVEFHEWVTKDGQEVGSTKDKSLYRITNELYKSTS
                     DSTLGERVNLSELYPDLQPVTPIQGLSRPLFTYLKTPGMNNKDINSPLGLSIFDNAKT
                     TIDFINRTYDEFMWEIKMGQRRVIVPEQMTQLTVQDSRGEITFKRRFDVEQNVYMQIG
                     AGNMDSGGIVDLTTPIRSSDYISAISEGLKLFEMQIGVSSGMFTFDGQGVKTATEIVS
                     ENSDTYQMRNSIVALVEQSIKELCVSMCELGKAVGLYNGKIPELDDISVNLDDGVFTD
                     RHAELDYWMKMVAAGFATQKRGIAKTLGITEDEAAKELAEINGELPPESDAELALYGK
                     GQQNTVGNSKDTVEGEDDGEV"
     gene            36179..36427
                     /locus_tag="VCA20_000101"
     CDS             36179..36427
                     /locus_tag="VCA20_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1833976.1"
                     /translation="MAKYKKKPVVIEAIRFTGSNYEEIREFIGKNTLCSDLSIVIPTL
                     EGDIVAQKGDYIIKGVHGEFYPCKPDIFNETYEVVSEA"
     gene            36430..37581
                     /locus_tag="VCA20_000102"
     CDS             36430..37581
                     /locus_tag="VCA20_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor capsid protein"
                     /protein_id="HEP1833977.1"
                     /translation="MSDTKKRPTINDQQFSLQMQGVSDIYAQMQTELFDSMIERLIRR
                     GSADLAENPYIWQLEKLNDMYMLNEDNLKIIVERTGIAEELLREVIANEGLKVYKDTK
                     EQLEEDMGKFPDGKIRNGVTDALEAYTQQAISDLNLINTTLPQSVQTVFKSVVEQTVA
                     QVVSGTKTSERALHDTIMNWQKKNFTGFTDSAGREWRADSYARAVIKTTTFKVYNDMR
                     TRPAEELGIDTYYYSIKRTARPSCSPLQGKIVTKEGSERKEHGITIHSLQDYGYGTAG
                     GCLGVHCGHYLTPFIVGVNELPDLPDFLQDLTPEQAEENARIEAKQRALERAVRNHKE
                     RLHYATTMNDDDLIQAERLKVRMYQGKIKALVDEHEFLSRDYTREKIYT"
     gene            37728..38291
                     /locus_tag="VCA20_000103"
     CDS             37728..38291
                     /locus_tag="VCA20_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HEP1833978.1"
                     /translation="MAFTTEELLKLGLTEEQAKDVFALHGKDLNANKSALETITQERD
                     SLKSQLQNTEAQLETLKADANTSAEQKEAIDKLRAEYDKYKADAAAELAQTQKVNAIN
                     LALKDTTAHNPSTLMKFIDVDAIELDENGTPKLDDILKGLKESDPYLFKADDDKPNPN
                     IVPPGNPVANDTGNTDPFQAIMDNYGK"
     gene            38312..39193
                     /locus_tag="VCA20_000104"
     CDS             38312..39193
                     /locus_tag="VCA20_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001139822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HEP1833979.1"
                     /translation="MAGNQNNPVRRYEKQYAGMLQTVFGIRKAFAKTLAPIQILDGVQ
                     ENAKAFSVKTNATPVVIGTYSTGANDGGFGDGSGAKSRFGNMTEVKYDNADVEYDYTL
                     SIHEGLDRYTVNNDLDAAVADRLKLQSEEQTRQMNTKIGKFLAENAGKTEALADFEEK
                     TIKSLFNKLDAYYTDLEVAVDITVYLRSELHSAIVDMASNTSAKGSSVSIDTNGLLKY
                     KDFILEKTPSKYLPTGTLAVFSPDGIVIPFVGISTARTIEATDFDGVKLQAAAKGGTY
                     ILEDNKKAVVKVTGTVV"
     gene            39204..39509
                     /locus_tag="VCA20_000105"
     CDS             39204..39509
                     /locus_tag="VCA20_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1833980.1"
                     /translation="MALYKATKNLVFQSLGKGVIVDEIIDLEPAYVEKVNKDLKLSFP
                     DVAAVLVAVDETVEDADGTTDDTPSETVDETVEDEKPKKSTRKKKIDTDTVADVAAE"
     gene            39551..39946
                     /locus_tag="VCA20_000106"
     CDS             39551..39946
                     /locus_tag="VCA20_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013794407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1833981.1"
                     /translation="MPYLTKDEFVKDLGFDDVTNFGKLAKRAEVAINLYTQGIYQQYI
                     DFDKEVEYRKSAVKLAMAFQIAYLDSSGIMTADEKQAMSSVSIGRTKIDYGSQHRISA
                     GQQFNLCLDAENALKQAGFSLIAGVDYDR"
     gene            39936..40262
                     /locus_tag="VCA20_000107"
     CDS             39936..40262
                     /locus_tag="VCA20_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HEP1833982.1"
                     /translation="MIDKRLLQDVVTVRKVEGKDDFGDVTYSDPLDIKPVRFDRSVAV
                     TGTNNSKTRQKVGTVYIYPKFASVTVDDSWLGAIVNDGARDYLVTGYQPNYLNGNIFS
                     YEVEVT"
     gene            40262..40612
                     /locus_tag="VCA20_000108"
     CDS             40262..40612
                     /locus_tag="VCA20_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HEP1833983.1"
                     /translation="MADVRVTVDLAGVEKKVSPQSMQRGKIAAGSEALLIMDSSIPLR
                     AGGGALRASGRVEPNGDASYNTVYARAQFHGTNGIVVFRKYTTAGTGKRWDKPLKANI
                     ERLRKAAIKGMGIR"
     gene            40612..41019
                     /locus_tag="VCA20_000109"
     CDS             40612..41019
                     /locus_tag="VCA20_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HEP1833984.1"
                     /translation="MQNNKNFQQVLLDHINNIEHIPLKARLDYFKDDKDDLVINALSG
                     GVIDKEYMDGTREVSLPFEIAIKCKENKKAIDTIWLINGDLSSLDIDLPSTDNSYTFL
                     SLGVDKPGINGKDEQGYFVYSMHVTAKLEITGG"
     gene            41023..41481
                     /locus_tag="VCA20_000110"
     CDS             41023..41481
                     /locus_tag="VCA20_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEP1833985.1"
                     /translation="MVRNKNAKRKHEIAPFDPQNPTKVPDESAWKRLAKYIETIDDET
                     DEETDDTGYYDGDGTPEEAVISVSGAYSVSGSYDPDDAAQAMIAAMKYETGDARRVWH
                     RVTESNEKKTYTQVANVSEIKAGSGDATSYEEFGCTLKWIKKPIIAGVGG"
     gene            41504..41872
                     /locus_tag="VCA20_000111"
     CDS             41504..41872
                     /locus_tag="VCA20_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1833986.1"
                     /translation="MANTFNLLGNDDSITFNLGDFTLTFTPTDAKSKAVSEKAVELKD
                     KADTIKEGNEWENRESIKGLLDDFFSTMFDADAPGKIYQAAGENTWNYLKVFLQMAEA
                     IMETKKKQENDETFKKYLAE"
     gene            41872..42459
                     /locus_tag="VCA20_000112"
     CDS             41872..42459
                     /locus_tag="VCA20_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1833987.1"
                     /translation="MFDISKKMDDKLVLNNTEYQLFLSFDRVLWCFDMWGKAHIPPEL
                     KPKLALAKLTDNESFKDMDTMEAMAIYQEVFEKHIKVIRAADHVVRYDIEGNVLPKRP
                     KEDPDNEEKPLFSIKYDGEYIFSSFMQAYNIDLIEEQGKLHWKKFNALLSGLPDGTKF
                     VEVMKIRAWKPSKRDSTKEIQRMRELQEEYALPDI"
     gene            42479..45841
                     /locus_tag="VCA20_000113"
     CDS             42479..45841
                     /locus_tag="VCA20_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1833988.1"
                     /translation="MADGKVTISVDLDGKQAQGGIEKLKGALGGLGSTFKSMLGANLV
                     GGALMSSFNAIAGSVKSVFSSAIDEGAKLQQSLGGIDTLFKNSADTVKNYASNAYKTA
                     GLSANEYMENVTSFSASLISSLGGDTAAAAELANRAMTDMSDNANKMGTDMQAITGTY
                     QSLARGNYAMLDNLKLGYGGTKAEMERLIKDASSYKDIQDELGISVEEGNMSFANMVK
                     AISVVQKKLDITGTTMKEASTTFSGSLSMMKGAFNDFLGNLTTGGDITKPLQALAESA
                     ATFLFGNFIPMIANVFKGLPTALSTFIASAKPALVNGLKGLLPEDALNSVTKVIDLIT
                     FSISDMVMAFQDFYQGFEKTGAIKALSSALSELLTAGLDLSEKLSGIIPWETIGAAAG
                     HVVKFIAQIIQAVAKFSQSMSGDTWRGVVTGIGGALVAFKAFSFLKSFNPFSFFKKGA
                     EEAIAGTTGSVKSAKSTISQVFSGISNVIKTTGNALKSTFQGIGKMFQGLGKTFEGVG
                     KGIGAALKGLMQGLRGLNPATLLSFGAAIGIAAVGIGAGIAIIAAGFALLASQGEGIA
                     IIVEAVGTAFGTFASMVIGAFAEAIVTVAGVLPIIAQSFNMMTPAIMAVGAAISMIIR
                     AFSSLAPVITALGTAISEIVTAITTGVADIATAVTPMVEILSSAFVQVVTVVSQAIVE
                     IIQALAPFIPAVSEMVQAVAPVLQALVEAFNNLISQISPIIDSIANLFKTLGEQITSI
                     LDSASGVVESFGSAIRNVLVGIAGIFDSIGNAALNAGKGFNQLAQGIERITKLNLLDM
                     GASLAAVAAGLGAIGATSGGLTSAGSSLSVIVQGLMTVQSVSAIASSSLASLGTVAVS
                     AMSMLVAGVQLAVSGTKSGMNQIVNAISLSANQMIQAGKQAGDGTTKGVVTGIRSGIG
                     QAGGAMHAMMIAIRDTGMQGVSSMRVVGSMISQGLAQGMMSSIGSVTAAANALVAQAE
                     RAAKAKAQIHSPSRLFRDAIGRFLPMGVAVGIEKNTKYVDKAMDGMYEHINAFNYKAE
                     DVIGVGKTKLSKVVQIKSDLESAIKATVEVAKEKSNELLAKALDVAEKAVERPAEMRL
                     DDGTLVAKTGDKFSSYQSEQLRRENRMKGIIT"
     gene            45838..47340
                     /locus_tag="VCA20_000114"
     CDS             45838..47340
                     /locus_tag="VCA20_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HEP1833989.1"
                     /translation="MTAIMTFNGVDLSRYLRITEIIRPIGNKRSVTTDDAPSLGVNIQ
                     QVKRGAKEHIIKFDMKNSDVMILEQLKHELAGVLDVTEPVKIVYGDEPDKYYMGIPVD
                     DITPDNLTRWFQRSEFKLLIPDGVAHSSTYRKFDSATVTGEKMVLNLRNDGNVPANPI
                     ITVKHNAENGYIGIVNSTGAIELGNIEEEDSQTYQQSEILFDYVSNNGIIKGFADGKK
                     NVAILNDNSQSFDTNLYVKNEFGRPHLALGNRGAGVGPNHAGSISWDIPLDSAGQSGA
                     LNEYIWWRQIFWAGAVNQYGFIKVMVSDEKGQFLYGVETYKRSIGVESEYNFLASDGK
                     GGFNVLKRWTFNATHLDSDNPFNAERGWSDIKRNDDQVQVFWWGSYPVFTIPEIKGKK
                     SAKIHVSIGAVGGKPAIHHAYIDSIVYRKDFVQGTKDIPNRYMIGSNVVVNCENDSVT
                     VDGLSKSSDLVDGSSWRLSIPKGESQLEIYCSSWVKNKPTVKVEFEERWL"
     gene            47340..50333
                     /locus_tag="VCA20_000115"
     CDS             47340..50333
                     /locus_tag="VCA20_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000927608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEP1833990.1"
                     /translation="MLLTIHDANLKKVAFIDNDKQETLNYYDDTWTRSLETGSSTFEF
                     TVYKKAIKSDTAINKTYNLLNERAFVSFRHNGKSYVFNVMTVEENEQTIKCYCENLNL
                     ELINEYANPYKATKAMSFVEYCNAMDLLNFTHLSVGINEISDQKRTLEWDGQDTKLAR
                     LLSLAKKFDAEIDFDTYLNADSSIKAFKVNVYRENDATHQGVGRVRNDIQLTYGKNLK
                     SITRKIDKTGVYNAIRPTGKRTVKNGKGEEVEEIVTIGSLGAWSENNKDGVREFYQQG
                     DMLYAPLSMQMYPSTFTSSTTNDQWIRKDMEVDSDSPSVIRAAAIANLRKNAYPALTY
                     EVDGFIDVEIGDTIRIYDDGFTPILLVQARVSHQKISFTNPASNQTTFANFKALENNL
                     SDGIQAAFERLFEASKPYLIKLSTDNGVIFKNQIGQSLVIPSLYRGGKPVVSGVTWRW
                     SLDGNITTGMTYLVQGSSVTDTATLTVAAYIGNDEVAVDELTFVNVLDGRDGGVGPKG
                     DPGNDGVAGRDGNKVYTSIYEMSANQSGIYLSDLTPTANPANLPPIGSIVIAPSGKMY
                     PIIALNPTSTPPSYTAGALVGNIKGPQGDNGQTPVVHWAYSDNADGTGLTTSDNGQRY
                     IGHYSDYTQADSTDKTKYRWADRWAKIEVGGRNYLRNSSFVDGVSYWQNNSGAEQFSW
                     RSVPEFATQLGRKGCIHLYSKGLPLDSLGIFQVIQDMQLSEQEKITLSFDVVGREGND
                     SGLMVIINNHLFDVRQIIPVSSIRTYSQFTKTSLERKALTFTIPKACDAISVIIIFHG
                     GITGDVVLTDFMLERGLVATSHQESPSDIEDRIGVKADQALTQERLNALSEQAGIMQA
                     ELEATASLDTVNNILKQIKDMKAADEATWAKVEKDLITHLQRVIKIETNLGDQAQRWN
                     AVDTFMQVSNDGLSLGKADGSSSMLFSPDGRITMFSSGTPVMYVDKGVIHIDNGIFSK
                     TVQIGRFREEQYHNNPDINVKRYVY"
     gene            50353..52374
                     /locus_tag="VCA20_000116"
     CDS             50353..52374
                     /locus_tag="VCA20_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1833991.1"
                     /translation="MAVFRYSGNWRGFLEGTSSTVSQDISGNSSVIKIDVWIGMDTGW
                     NIEFGNTYGNTVTVTCDGQYQTIAVGPLYLNSSKKHLGSVQFRVGHNADGTKSAGIGL
                     SSNMSNISYGTLNFGNASGNWVHGLATIPRSSSVSVGTGVIGSAVTININRQSSSFKH
                     IVRYHWGNKQGTIASNVDTSTTWTIPLDFANDIPNATSGIGTIFVDTYSGSTKTGTQQ
                     VAFTASVPDSIKPSLTGFTLIDGNTAARTLIPGEQQFVQIVSNIAVHFGQATGTYGST
                     ITSYHAEIVGKNQSTSQNGGSLGIMNYHGQVTIRARVTDSRGRTSNTIERTVTVLEYF
                     APAFNFSVERSGATSSTFSILRNARIAPLTVGGSQRNTMTLTFRVAPADSNNYTTDNG
                     PASGTFTTLASLTNSLANLSGTYSSDKSWDVIGILEDKFTRSEFKIKVSTEAVVFSYE
                     KGNRFAVGKIVDTNLPGGSVESTGGYYLNGKPIQNHQLTENTGQAIRAFSDWNNYVDT
                     GFYMGDNILNQPPFDGQHPWKYVRTTKHNSLYALQESVDFYGRSYSLRVKENGKWYPW
                     KQLVMSDHPNLINTGWQSAGFPGTYYKRVGDILTIKYDFRGNGSTMNIGSIPSDIWVA
                     PQSYMLIIAKWSISGSDNSHVQINQGTGGLNVLSTGNGVEYKGQITIMI"
     gene            52387..52821
                     /locus_tag="VCA20_000117"
     CDS             52387..52821
                     /locus_tag="VCA20_000117"
                     /inference="COORDINATES: protein motif:HMM:NF018771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1366 domain-containing protein"
                     /protein_id="HEP1833992.1"
                     /translation="MKFKFLTKSTEWLGSSPHRTIVVVGNEEGASIPYAFDKEAINLS
                     DSELFDMAMEKMYQENFPNRAEDEKFNAIGKRLAKIDDITEEATKNLEKVKEQVTMSA
                     SSRAAFLQIVMTLYGKGLLTDEDLLQTGLFDDEVVEETLEVI"
     gene            52844..52999
                     /locus_tag="VCA20_000118"
     CDS             52844..52999
                     /locus_tag="VCA20_000118"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1833993.1"
                     /translation="MMIKLYAIEIFEGNIKYKDLPFSDIIKNKIKAYLTKMVEDKEIL
                     AELISEE"
     gene            53003..53281
                     /locus_tag="VCA20_000119"
     CDS             53003..53281
                     /locus_tag="VCA20_000119"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1833994.1"
                     /translation="MQIKPEHVYALVGFVSTVVGLWTNFSAKITKQENRITILEKDIE
                     NLKEFKESANRRLDSHDEQNKAILVLAEQVKSMGEDIRDLKRVIMKEG"
     gene            53287..53529
                     /locus_tag="VCA20_000120"
     CDS             53287..53529
                     /locus_tag="VCA20_000120"
                     /inference="COORDINATES: protein motif:HMM:NF016419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEP1833995.1"
                     /translation="MKINWGVRLRNKTFWWTLVPLLVLLSQQLGFNWVPENWESTFAT
                     IMSILTVVGIINDPTTAGVSDSKQALDYYEPKADKR"
     gene            53617..>53783
                     /locus_tag="VCA20_000121"
     CDS             53617..>53783
                     /locus_tag="VCA20_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HEP1833996.1"
                     /translation="MTINLETSIRWMSDRVGKVSYSMDYRNGPNSYDCSSAVYYALMA
                     GGAISAGWAVDT"
BASE COUNT        16549 a         9977 c        12340 g        14917 t
ORIGIN      
        1 tcatattggc aaacgtgatt taggaatggc taatccgatt agcggacaaa tgcgacaaat
       61 acaaacaata gcctgagtgc cttgagcaag cacaaaaaac acccatggcg gcaaccgtgg
      121 gtgttttctt tatgcactta tctctttgaa cttttaagca caatgattat aacatatgtg
      181 tctttagttt tcaagtgtcc agtccaagtc aggtcctaca ggaacgatgc cggtcggatt
      241 gatggtcttg tgactgccgt agtagtgctg cttgatatgg tcaaagtcaa ccgtttctgc
      301 aataccagca tgattgtaaa gacgttttgt atagttccat aggtttggat agtcaaccaa
      361 ggccttgata ttgcatttga aatgtccaaa gtatacaggg tcaaaacgta ccaaagtggt
      421 aaagagtcga atatcagcct ctgtcagctg attgcccacc agatagtcct tatctgccaa
      481 atgctcttct agtttgtcca aggcagcaaa gaggtttttg acttcttttt catagacttt
      541 ctgattggtt gaaaatccag ccttgtagac tccgttgttg atatttggat agataaagtc
      601 gttcatggta tcaatctcgg cttgtagatg ttctggatag tagtcatcat agttgcctgt
      661 aatgtcattg aaagcagtgt taaacatgcg catgatttca gcagactcgt tgctgacgat
      721 tgtattggtt ttcttatccc acaggacagg aacggtcaca cggccagaat agtgtggatc
      781 agccttcaaa tatacctgat agagatagtc gctgttaaag agggtatcct tgataacacc
      841 aggaccatct tcaaatgtcc agccattttc taacatgagg ggatgaacga cagacaggga
      901 aatgtggtct tccaagcctt tcaattttcg cataataagc gttcggctag ctcaaggaca
      961 ggccagagaa atgtagagat ggtagcgacc agactcagcc ttgaaaccgc cctctcctgt
     1021 tgggcctgtc tgtccatctg gcgtaatcca gttgcgaaat tgtgtgacac tccgaacaaa
     1081 cttgccacct gtagacttgg tatcatacca cttgtccacc catttgccat cttgtaaaag
     1141 tcccatgaat tttctccctt actgaaaaaa tgggaaatcc agtaggatga ttggatttcc
     1201 tttttaaaaa atatatcatt aactagtatt ataaactttt ttcaaaaaga gtcaagctat
     1261 atgatttgtg atacaatagt cccatgagat tggacaaatg tttagagaaa gctaaagttg
     1321 gctcgcgtaa gcaagttaaa aaacttttca aatcccagca aatcaagatt aacggacaag
     1381 cagctcaaag cctaagccag attgttgacc cagagttaca aactatccaa gtttcgggta
     1441 aaaaagttgc cttagaaggt tccgcctatt atttgctcca taaaccagca ggtatcgttt
     1501 ctgcagtgac agaccaggag catcagacag tcatcgactt gatttctccg caagacagta
     1561 gggaaggtct ctacccagtc ggtcgcctgg acagggatac cgaaggccta gtcctcatta
     1621 ccaacaatgg tccgctcggt tatcgcatgc tccatcccag ccaccatgtg gacaaggttt
     1681 actatgtgga ggtaaatgga tgcttggctg aggatgcttc aaaatttttt gcctcaggtg
     1741 ttactttctt ggatggaaca cggtgtcagc ctgtagattt gactgtcttg gaggcttctt
     1801 tggaccatag cagagcgacc atcaagctgg cagaaggtaa gtttcatcag gtcaaaaaga
     1861 tgtttttagc ctatggtgtc aaggtcactt atctcaaacg gatttccttt ggtggttttg
     1921 aactggggga tttggaacga ggtgcataca gacaactcac accaaatgaa atggaacatt
     1981 tattcactta ttttgactag aggaggatat ataatgagca tttatgattt tacagtacaa
     2041 aaacaagatg gagcagatca gtccatggct gagtatcaag gacaagtttt acttatagtc
     2101 aatacagcgc caggttgtgg attggcacca caatacaagg aactacagga gctatatgat
     2161 agctatcgag gccaaggttt tgtagtgttg gatttccctt gtaatcaatt tttaaatcag
     2221 gctcccggtt ctgcggaaga catcaatcag acctgtagct tgaattatgg tacaactttt
     2281 cctcgttttg ctaaaattgc tgtgaatgga tctgaagcta gtccgctata tcgctatctt
     2341 aagaaagaaa aatcaacctt attaggaggt cgaatcgagt ggaattttac caagtttttg
     2401 gtgaatagac aagggcgtgt ggtcaaacga tatttaccaa caactagtcc attaaaactt
     2461 aaagaggata ttgaacttta cctagaaaaa taacaagaag gctggagtca tctagccttc
     2521 ttgttttagt aaccacagaa aatagggagc gccgataagg gagacgatga tgccggttgg
     2581 gatgcctgtt ccgacaagta gtactcgtcc tatggtatcc gctagaagta gaatgaagct
     2641 tcctagtatc atagtagctg gaatgatgat acgatggtca tttcctaacc atttgcgcac
     2701 aatgtgacca gcaatgagac caacgaaggt gatattacca actaaaatga cactaagaga
     2761 ggctagggcg gtagccaata ccaaggtagt ccgcctttca cgctgtaagt ggagtcccaa
     2821 agccatggct gtctggtcgt ttagattcat aatattgagt gagcgacttc gactaaacgt
     2881 tgccatccac accaatagaa gaagaggagt atagattgcg atagttttcc aatctccacc
     2941 atttactcga ccgctgagcc actggacaat gtattccatc ttaaagtcat ctaattggct
     3001 gatgatgctg accatgatac cagacagcat gctcgaaatt ccaactcccg tgataattaa
     3061 gcgtgcagga tggatgccgt acagcttctt acgtgcaatc cagtaaacta gaaaaatggt
     3121 taatccaccg cccatcatag caaatagagg catgagactc aataagacag ggctgatttt
     3181 tgaagagaga ccgacaaata gtgccacaac aagaccagct cctgcgttga ttcctaatat
     3241 tccagaatct gcaagaggat tgcgggtcaa agtttgtagc aacaaacccg ccaaagccag
     3301 agaagcccct ccgataatgg aggcgattat tctaggtaag cgaattcgtc caatgataag
     3361 tagagtcgat gaactggcac gcccactcat gatgtcgatg agatccgaaa aggatgaatt
     3421 gctatagccg atagataagg aaagtaggaa aatgacaaat aaaaggatta gcaggaatgt
     3481 gaatattttt agatagttgt gatgtcttat caaaagtgtt tctcctttct aatcaaccat
     3541 aggaagcaag ggagaccaat gatactgatg actgcgctaa tgggagtttc aacaggtgga
     3601 ttgatagaac ggcaaaccaa atctacccag atgaggaaac tagcccctgc aaaggcagtt
     3661 aatggcaaga gtagcttgta atttttccct gtaaacatgc ggataaagtg aggaataatg
     3721 agcccgataa aagaaagcga gcccacaaga gctaccgcag aagctgagag tagaaggaca
     3781 attcccaaca aaaccaaggt catcagtaag gttcgttgac ctagattttt agcaacggtt
     3841 tcgttaagac tcaggatggt cagctgatgc gaaaagagtt gagcaaggat gaggccgatg
     3901 atgataagag gagcaatgat ggccagcatc ttccagttta cctgtaccaa tccccctgct
     3961 tgccagccaa ttactgcagt tgataaatca aagtagatag taatagcctg accaatagca
     4021 gaaaatagac ttgctaccat ggcgccagat aaaattaaac gtagttgatt gtagcctttt
     4081 tgcacctgat aagctagacc aaagactaga ccggctgcca gacaggagcc cagtaaacat
     4141 attaataaaa tttgactgta gtgcaaacca ccgaacagag catatgcaac aatgagagca
     4201 agtcctgctc cagcattgat tcctaataag ccaggatcgg caatcggatt gcgtgtaatt
     4261 ccttgcatca ttgccccagc ttgggccatg gcagctccca ccaagatggc ggctaccatt
     4321 ctaggcaagc gtaaatcgat gataacatcc tgagcggaag aatctgtcag tggatgcgtt
     4381 agggtttcta tcagttgttg gtggctataa gaaatagctc caaagcgtaa actaagatag
     4441 actccagcta tgaaagaaag acagatgatg aaaaaaacaa gccaaatatt ctttggcttt
     4501 gttttgggat aacgagaact tactagtttg ttcatatatc cagtcctttt actgaagttt
     4561 ttgaatttct gagacaaagg cgtcaatttg taaatctaaa gacatcggat cggagaagta
     4621 gaagaggtta tagtctatct cgatgacgtg accattcttt acagcaggta gatttttcca
     4681 aatatcactt tctttgagag aggcagctgc ttcttttgtt ttatcattga cgttaacaag
     4741 gacatagtcg ccaacatatt ctggaagagc ttcttgagat accccaaacc agccttcttt
     4801 attgatgatg tcttcttgaa cctttgcagg agcggcaaaa ccgagagcgc gatagattaa
     4861 ttctccacca cgaccaaagt tattaccata aaggaagatg ttcttatcga agaagtccat
     4921 gacggtaaat gttgaacttg tatcaatcaa tcctttcaat tcctctttcg cagtagcaac
     4981 tttcttatcc cattgatcta accaggcttg tgcttccttt tcttttccaa aaatctgacc
     5041 aatatctgtc agcattttta agtagtcact ctttccataa gtgatttcaa taactggagc
     5101 aatttctttc agtgtttcta agttatcatc accagaaaat actagaatga catctggctt
     5161 ttgtgcggca atcgcttctg tatctgccgt tgtcaattgt ggaacatcct ttagtttgtc
     5221 tccaaagacg ggactatttt tctctaaatc tagactatag ctagatacat caattcctaa
     5281 ttgtaagaga tagcctgtgt aagagtaagc aaagttgaca acttttttag gattttttgg
     5341 aatgtcaccg tggtaagtaa tgccttcaat ctttggcatg ctagacagtt ctacgtctgt
     5401 tgaagaggaa gaggtagaac aagctgttaa gaaaaccagc ccaacaaata aactaaaaat
     5461 agctaaaaac tttttcattt tttctcctta ttattttttt aaatcgtaag ttagcaaaat
     5521 agggtaatct tgaccagcaa cctgcatcaa ttgaggttca atgctaaaaa tgtccctcag
     5581 tatctcagtt gtcatcatat tggacacaga cccatggtag cgaatatctc cctctttcat
     5641 ggcaatcata taatctgaaa atcgagctga taaattgaca tcgtgaagga ccatcacaat
     5701 ggttttctgt cggctttgat tcagttcttt taaaagttct aagacttcca attgatgatt
     5761 gagatcaaga taggttgttg gttcatctaa gaaaatggta tccgtatctt gggcaagtgc
     5821 catcgcaatc caaacccgtt gcctctgacc acctgataaa tcatcaactg gtaacctagc
     5881 gtagggagcg gtctgggtga cttccaaggc ccagttgatt ttctctctat cctgatcagt
     5941 gagatgcccc agcccatttt gatgcggaaa gcgaccatag gagaccagtt cataaacgga
     6001 aatcccttct gtcgcttcta agacctgagg gagcagggcc aatttttttg cgacttcttt
     6061 ggttggcagt tgtgaaatgg cttgtccatc taaatagata gccccttctt gaacaggaat
     6121 aatccttgtc aacgccttaa gcaaggtcga ttttccacag ccattggcac caatgattgt
     6181 cgttatattt ccttttggaa tacttgttga taattcatca ataataatcc gattgtcgta
     6241 agagacttgg atattttccg cttgaatcga agacacaaac tcctcctttc cagaatatga
     6301 ttcattaata atttgttccg accagtatta tatcaaaaaa attcaccaaa acaaagagtt
     6361 ttctgaaaat tttaatttaa tgagaatcgt tataaaaaag tccttgtctg tgaacaagga
     6421 ccgtatgtta caaatattgt tttgctactt ccaaatcacc gtcataggcg accttttcgc
     6481 cgtttacaat ctggtaggcg tcggctccct tgcctgcgat gatgaccgcg tcccgagaag
     6541 aagttgtctg gctcatagcg gtacggatag cttcttcccg gtccaagata aagtcacttg
     6601 ggcgagtcat atgggaacgg atttgctggg caatttcagc gggatcttca cggttggggt
     6661 catcagctgt cagaattacc tcaacctgcg gatagtcatt gagcagaaga ccgaagtctt
     6721 tgcggcgact ttctcccttg ttaccaggag ctcccaagat caaaaacacc ttgcctgtct
     6781 ggtgctctaa gacgacatca atcagcttct tgacactatc gccgttatga gcgtagtcca
     6841 cgaatacctt ggcaccgttt tgctgggtca ggacttccat acggccagga acattggttt
     6901 gggcaattcc tgtgtgaatg tcctccagac ttgcacccaa acggagacag gccagtcctg
     6961 ctgcgatggc attttcttgg ttgaagccac caatcagctg gatgtcgtaa tggccagcca
     7021 atttgccagt cgctgtgaag tcaaaacctg ctgattggct aatctggttt tcagaagtcg
     7081 gcccataaaa gtcatggtct ttggagctaa cttgctcttt cacgacttca aaatggtcca
     7141 ttcctgcatt gacaataacc gctctgctat tgtccatcaa aagacgctta tggtagaagt
     7201 agtcctcaaa agttggatgc tcaatggggc cgatgtggtc ggggctgatg ttgagaaaaa
     7261 cgccgacgtc aaaggtcagc ccgtaaaccc gcttgaccag ataggcctgg ctggacacct
     7321 ccataatcaa gtgggtcatg ccgttcttga ccgcctccgc catcatggca aagaggtcca
     7381 gactttcggg cgtggtcagg gtggacttga agaaggtctt accgtccagc gtggtgttca
     7441 tggtcgaaag catggcaggt ttcttgctct gtttaaggat gttaaaagca aaataagccg
     7501 ccgttgtctt acccttggta ccggtgaaag ccaggagttt gagctgtttc tggggatgac
     7561 cgtgaaactc catggcaatc aggctcatag cctgcttgat gtcgtggacg atgatagcag
     7621 gaatgccgac ctcatagtcg atttcagaca cgtaaaaagc cagcccaacc tcgatagcct
     7681 tttccagaaa ttccttctta aaggccaaac ccttggcaaa aaagagggtc cttgggctaa
     7741 cagttcggct gtcatagctg agggcgtcaa actggacatc cgtccatgtg ctgtcagtct
     7801 ggtcattctg tttgatatga cagaagttag catccgcctt caatatttct aatacacgtt
     7861 caattgtaat catgcttcta ttgtaaacct atcgaggata tttgacaagt ctgggctatg
     7921 taaatagtgg gtattttgaa cgtaaatttg aatgaatatg acatttcgtt ctgacgcaaa
     7981 tttgtgctat aataggaggg attgaaaagg agaagtttat gttttattct tatctacgaa
     8041 cactattaac attcttgttg tgggccatca atggcaatat tcactatcat gatagagaga
     8101 agattttgcc acaggaggaa aattatattc ttatcgctcc gcacaaaacc ttctgggatc
     8161 cagtctttct tggctacgca gctgctccca agcagttcat ctttatggcc aaaaaagaac
     8221 tcttcaagga ccgcggtttt ggttggtgga tttcaaaatg tggagccttt cccattgacc
     8281 gtgacaatcc tggcatggca gccatcaagt atccagttaa catgttgaaa aaaagtgacc
     8341 gttcgctggt catgtttcct tcaggaagtc gtcattcatc tgagctaaaa ggtggtgtgg
     8401 cggtcattgc caagtcagcc aaggtcaaac tcatgccagc tacctatgtg ggtcccatga
     8461 ctatcaaggg gctcttagct ggtgagcgaa tcgatgtggc ttttggaaat cctatcgatg
     8521 tttcggacat caagcgtatg gatgatgcag gtacagcaga agtaactagc cgaattgaag
     8581 ctgaattcaa acgcttagat gagcatgcaa cttctttcca aaccaagaaa aaaccaaata
     8641 tcttgactta tatctatcgt atcccagttc ttttgctggt tgctcttatc ttgggattga
     8701 cctatgcctt tagctatatt gccagcttct tctggcaacc aagtactcag ttggataaaa
     8761 aataagtaaa tgaaactcgc agcttgcggg ttttttgtat tttttacgaa ctatataggt
     8821 ggaggtaatt tatggaaacg attaaaactt atatagaaat gcttaaagaa tacaagtggc
     8881 aaattgcttt gcccgcagtg gctggcttgc taatggcgac gttcttaata ttcagtcaac
     8941 cagccaagtc tgaccagaca ggcctgacag attttccgca gaccgcacaa acttctagca
     9001 gctctaactt ggtcgaggaa accagtacag aagtaagtga ggaacccagc cagctggtcg
     9061 ttgatgtcaa aggagcagta gcaaagccgg gactctacac tttagaagct gatgcgcgtg
     9121 ttaatgatgc agttgaagca gctggcggct tgaccagtca ggcagaccca aagtctatca
     9181 atctggctca aaagctcagc gacgaagcgg tggtctatgt ggcaagcaag gatgaaaata
     9241 tctcggtggt ggccagcacg actaccagct ctgctatgtc ccaagaagaa aaaaacacca
     9301 gtctagtcaa tctgaatacg gcgactgagg cggacttgca gaccatttct ggtattggtg
     9361 ccaagcgagc gacggacatt atcgcctatc gtgaggccaa cggtggcttc aagtcggtgg
     9421 acgatctcaa caatgtgtcg ggcatcggcg acaagaccat ggaaagcatc cggccttatg
     9481 tcacggttga gtaagctccc ctgccagccc attcacctgg cagtcttggc ggtggcagct
     9541 tactttgcag tccactcgtt ttctctcttg acaatgggcc tgttcagtct gttactagct
     9601 gtctttggtg ttcggcaagg taagatggtt ttcctaaaaa cgctgccgct tttagcccta
     9661 tgtggtctgt ttttcggcta ccaaaaggtc caatgggagc gggcagctca gtcagccaca
     9721 gagcaagtga taactgttca ggtcattcca gataccattg aagtcaacgg ggacagtctg
     9781 tcctttcggg gtcgggctga cggacaaacc tatcaggttt tctacaaatt agccagtcag
     9841 gaggagcagg cttattttca aaagctggca gacctggtcc agctagaagt ggaagcagag
     9901 gtcagcctgc cagctgctca gcggaatttc aagggctttg actatcaggc ctatctgaaa
     9961 acacagggca tctatcggac agtcaagcta acggcgatta agaagattgt ccctgtccag
    10021 tcttggaatg tttttgactg gctgtcaact tggcggaggc aggccttggt ctatgtcaaa
    10081 accaattttc cggctcccat gagtcactac atgacagggc ttttgtttgg ggagctggat
    10141 agtgattttg accagatgag tgacctctat tccagtctag gaatcattca tctctttgcc
    10201 ctgtctggta tgcaggtggg ctttttcatc gacaagtttc gttggatttt gttgcgtctg
    10261 ggcttgacca aggaaacagt ggataagtta caaattccct tttcccttgt ctatgctggt
    10321 ttgacaggct tttctgtgtc ggtggtgcgg tccttggtcc aaaaaattct gggtaatcta
    10381 ggcttgcgga agttggataa ttttgcggtg acggtcttta tctgtctgtt gatactgccc
    10441 ggatttttac tgacagcagg aggagtgctg acttttactt atgctttctt gctgacggtc
    10501 tttgattttg aggatttggg gcaggtcaaa aaggctgcgg tggagagcat cagtatttcg
    10561 ctgggaattt taccgattct catgacctat ttctatgcct tccagccctt gtctatcctc
    10621 ttgacctttg tcttttcctt tgtctttgat gtcctactct tgccaggttt gtcggtcatt
    10681 tttctcctgt cgccagttgt caagatgact tgggtcaacg gatttttcgt ctttatggag
    10741 aaaatcattg tctgggtggc tgatttggga ttgcggcctt ggatactggg caagccgtct
    10801 gagctggtcc tcttgctttt gctggtcagc ctcttcttgc tctacgattt ccacaggaga
    10861 aagaaatggc ttctaggact gagcttggtc ctcgctctgc tatttttcat caccaaacac
    10921 ccgctggaaa atgaggtgac ggtgctggac atcgggcagg gggacagcat ctttttgcgg
    10981 gatatgcggg ggcggacagt tctgattgat gtgggcggac gagtcgattt cgcggccaag
    11041 gaagcgtggc aggagcggtc tcggcaggca aatgcagagc ggactttgat tccctatctg
    11101 catagtcggg gtgtggacag gattgatagt ttggtcctga cccacaccga cacagaccat
    11161 gtgggcgatg tgctggaagt ggctaagcag gtgcaaattg gtcggattgt cgtgtcgccg
    11221 ggaagtctaa cggtgcctga ctttgtagcc accttgaaag aaattgatgt tcctgtccat
    11281 gtggcgaaag tgggcgaccg cctaccaatt tttgacgcct atctggaagt cctctatcca
    11341 gatgggacag gcgacggtgg taataatgat tctattgttc tctacggtcg cttgttggaa
    11401 actaactttc tctttacagg ggacctggag caaggggagt tggacttgat agaaacctat
    11461 cccaacctgc ctgtcgaagt cctcaaagcc ggtcaccacg gatctaaagg ttcttcctat
    11521 cctgaatttt tagaccatat cgaagccaag attgccttgg tatctgctgg agaaaataat
    11581 cgctacaaac atccacacca agaaaccttg gaacgatttg atagtcgcaa tatccaagtc
    11641 tatcgcacag accagcaagg agctatccgc ttccgaggtt ggagggagtg gaggattgaa
    11701 acggtgaggg agtaactatt gaaatgtagg caaggtggaa taggtttcac tcccactgta
    11761 gctgttttct tttttgcgtg gtataatgga gagaagaata cggagagatt atgacagtaa
    11821 ttgaacagat tgaaaaatta aaaaaagaga aattagagct tctgaccgtt ctatgcggtg
    11881 aagacatcgg acagtaccaa atagcgaaag acttacttct gcggaagctt gattttgatg
    11941 cagcagattt aggttttgcc tattttgata tgtcggaggc tgattatcat caggtagact
    12001 tggacctagt ttctcttccc ttcttttcag atgaaaaagt agtgattctt gattattttg
    12061 cagatttaac gacagataaa aaacggcatt tgacagatga tgaactcaaa cagtttgagg
    12121 cctatttgga aaatcctgtc gaaacaacac gtttggtcat tctagcaccg ggtaagttgg
    12181 acagtaagcg tcgtctggtt aaactcctca aacgagatgg actagtatta gaagccaatc
    12241 ctctgaagga gatggatttg aaaaatcatt tccaaaaaga gataatgcgt cttggattgc
    12301 agatgggagg agaggttttt caatatcttt tagaaaagtc caattttgat tttgcagaaa
    12361 tcagtaagaa tctagccttt cttcaatctt atatgggaaa agagacgatt gaaatttcag
    12421 atattgatgc ggctatccca aaaacactac aagataatat ctttgattta acacagttaa
    12481 ttctacaatc caaggtagac gaggctcgca acttagttcg tgacttgcgg ttgcaagggg
    12541 aggaagaggt taagttgatt gccattatgt taacccaatt tcgaacctat ctacaagtgc
    12601 aaatactagt agagcaaggc cgaggggaac agcagatagt agctgagtta tcaactatta
    12661 tggggcgtaa ggtgaatcca taccaagtaa agtatgcttt gcgtgattca cgccatctgt
    12721 ccattggatt tttgaaaaaa gtcgtcaagc tcctaatcga tacagatttt caaattaaaa
    12781 caggccaatt tgataaggat tatttatttg atttgctatt attaaaaatc gcaactacat
    12841 agtaaaaaga gtattcttag attgagggtc aaccgtggtt tcagacaaat aaattgaaaa
    12901 ggctctcaca ttggagtaga atagaactat cacttagtat aggagaaaac gaaaatggca
    12961 attattttac cagaccttcc atacgcatac gatgccttgg aaccacatat tgatgcagaa
    13021 acaatgaccc ttcaccacga taagcaccat gcgacttatg ttgcaaatgc taatgcagca
    13081 cttgaaaaac atcctgaaat tggcgaagac ttggtagctc ttttgtcaga tgtggagcaa
    13141 attccagctg acatccgcca agctttgatc aacaacggtg gtggtcattt gaaccatgct
    13201 cttttctggg aattgctttc acctgaaaaa acagaaattt cagcagaatt ggcaacagat
    13261 attgatgcaa cctttggttc atttgatgct ttcaaagaag ccttcactgc agcagcaaca
    13321 actcgtttcg gttcaggttg ggctttcttg gttgttaata aagaaggcaa gttggaagtt
    13381 atctcaattg ctaaccaaga cacaccaatc atgcaaggtt tgaaaccaat cttggcattg
    13441 gacgtttggg aacatgctta ctaccttaac taccgcaatg tccgtccgaa ctacatcaaa
    13501 gccttctttg aagtaatcaa ctgggacaag gttaacgaac tttataaagc agctaaataa
    13561 gaacagttct cttccgtttg gaagagattt ttaagttgtg aggagtattt tggagagaac
    13621 tgtgtaaggg aagagggatg atttttcgta ttatggataa aaacaacaag atattttcag
    13681 aaaaatgatg taaaagtaag aataaacgaa tatttttata attttttgtt ataaatgact
    13741 gatttaatat tgaaaagctg taaaaaaagg attattatag atatgtcaat atgaaaaaat
    13801 agaaggagca ttgttttgct tatgaaaaag aatattcggt tgaaaagcag tatactagct
    13861 cttgtagctg gttttagtgt tattgcaaca caagctgttt tggcagatga attagctgtc
    13921 caaattatgg gagttaatga tttccatggt gcgcttgata tgacggggac agcgcgattg
    13981 gaaggggaaa cagttcgtaa tgcaggaact gccgctttac ttgatgctta catggatgat
    14041 tcacaagcag aatttgaaga aacagcagca gaaacagaga cacctgcaga gtctatccgt
    14101 gttcaagctg gggatatggt tggtgcaagt ccatcgaatt ctggactttt acaagatgaa
    14161 ccaactgtaa aagtctttaa taaaatggat gttgaatacg ggactttggg gaaccatgag
    14221 tttgatgagg gacttgatga gtataaccgt atcatgactg gtgaagctcc aaaagaaggt
    14281 cagtttaatg agattgtaga taattatact catgaagctg ccaaacagga gattgttatt
    14341 gctaacgtta ttgacaaaga gacgggtgaa gttccgtatg gctggaaacc gtatgctatt
    14401 aagactattc ctgtaaatga taaggaagca aatattggct ttattggtgt agttacgaca
    14461 gaaattccta atcttgtttt gaagaaaaac tatgagcagt acactttttt gaatgaggca
    14521 gagacgattg ctagatatgc gcgtgagtta gctgaaaaag gtgtaaatgc gatagttgta
    14581 ctggctcacg ttccggctac aagcaaggat ggtgtggctg ctggtgaagc tgcagatatg
    14641 attgctaagc taaatgaaat ctatgctgaa cactcagttg accttgtatt tgctggccat
    14701 aaccatgtct atacaaacgg tacaacgggc aaaaccttga ttgttcaagc tacctcacaa
    14761 ggtaaggctt acgcagatgt tagggctgtt tatgatacag atattgccga ctttaaagct
    14821 gttccgactg cgaaaattat tgcagtagca ccagggcaga aaacaccaag tccggaaatt
    14881 caggcaattg tagatgaggc aaataccatt gttaaaaaag taactgagca aaaaattgct
    14941 acagctagtc aagcgacaga tatttcacgc gaagtgaatg aatttaaaga aagtgctgta
    15001 ggtaatctag taacatcggc tcaattagct atcgctaaga aatcgggtta tgatgttgac
    15061 tttgcaatga caaacgatgg cgggattcgg gcagatttga aggtccaaga agatggaaca
    15121 gttacttggg gagcagcaca agctgttcaa ccatttggga atatcctaca agtcgttcaa
    15181 atgacaggtg agcagattta tacagcctta aatcaacaat atgatgaagg tgaaaaatat
    15241 ttccttcaaa tgtctggaat taaatatatc tacacgaagg ctgacaatcc aacggaagaa
    15301 aatccttata aggttgttaa agccttcaaa gaagatggga cggagattgt tccgacagaa
    15361 acctatacac ttgtcatcaa cgacttctta tttggtggtg gggatggctt ctcgattttc
    15421 aaagaagcta aactgattgg tgctatcaat ccagatacag aagtatttgt ggagtacttg
    15481 actgatttag aaaaagcagg tcaaaccatt agtgcaacaa ttccaggtag aaaagcattt
    15541 gtagagaagt acgtagaaga accaaaagca gaagaaaaag aagataatgc tgggacaact
    15601 actgatgtga aaacacctga gaaagcaaat gacggtggcg atagtgtaac aaatcagaaa
    15661 gcaaacgagc aaccggcacc atctggaagt atggctccta tttcaaataa gaaaactgaa
    15721 aaagcatcag gaaatcaaac gcttccaaat accggtcaag aagccctagg ctcacttctt
    15781 attagcttgg gtggcttagt ttcactcgga atggctgtct caatgagacg taaggaaggg
    15841 gagtaggaat caagcataaa aacgacaaac tttcgaagta cattctagaa ttacttcttt
    15901 tagagactat tatgagagaa atacataaaa acaattgtgt ttcatcttag agtaggttct
    15961 gctttttgta aaaatttgaa aaacttatat aaccttgctc ttgcaatctg ctctcttctg
    16021 gggtacaata gagaagcaga cttcccttag ttaaatggat ataacaaatt cctcctaaga
    16081 attagttgca ggttcgattc ctgcagggga gatgaaaata cagtaaaaac ccttgataca
    16141 caagggtttt taactttctt gccccaaatt agccccaaat ttttccatta aatttctgac
    16201 tttgtcgaat gattcttctt gtttggcctt aaatagatgt gaataagttt ttaaagtttc
    16261 agttgcatcc ttgtgtccta ctaacttggc aatggtcaca acgtccacat catgatagat
    16321 cagccagcta acgtaagtat gacgtaagcc gtgtacatta aatgtttgac gtgtcttttt
    16381 ctttaaaatt ttattttcgc cagtccccgt caattttgta aataatcttt tatctggatt
    16441 gtctatatat ccaactttca tgtactcgtc gtatgctttc agccactcac tatcaaatgg
    16501 cacatctcgt tctgattgcg ggttcttagt aggtccccaa cctttcttct ttccataaac
    16561 cttgtaagtc ctgcggattc gtaaacacat attttcgcgg tcaacgatag actctgtaat
    16621 gcctgctgct tccgagaaac gaagtccggt ttttccgata gtgtacagga agaagtggga
    16681 ctggtacttg attgtctttc gataatctgt gattaattgt tcgtattcat caagttccag
    16741 gtacttatcc tcctctttct tggattcgac atcggagaaa actttgacaa tttcggtaaa
    16801 atcttttttt aaaataccct gatggatagc aactttaatt gctgccctta tatgcgaatt
    16861 gaaccttttg acgctatctt tcacataaca ttttgccaat tcgtttataa tgttttgata
    16921 tgaagtggcg tttatttttg aaagtttcgt ttcatggaaa tatctagtaa ttagcttaag
    16981 ggtatattca tacttgccaa acgtttctgg tccgacatga ggcttcttgt ggataagcat
    17041 ccacttttca aagtactcag caagggtaat gttcttatct tccacaatgc cattggacag
    17101 ctcaatttca gcttgtgagg cagcctgaac agcttctgcc ttggttctat atcctgactt
    17161 agatttctgt ttatatgagc cgtctggggc tttataggag atacggtatt cccaaccgtt
    17221 atcccttttc ctaaaatatg ccattgattt accctttcaa atttgataaa atgggtatag
    17281 taaagagacc tactgcagag caggtttttt actgtacatg ataccctaca ctcaaggttt
    17341 ggcgacggag agtgtgggga tttttttgtt attcgatatc taattgcaag acttctttga
    17401 tttttgcttt taacttgtcc aaatctgtct ggtcgagttg gtaggctggt ttgtagattt
    17461 tctggaatag tgcctttcct gatttgtcag tgaggggaac tccagaggag tctacttgct
    17521 ttttggtctt gtagatgatt ttgcgtttgt caatttcttg tatttttcgg acataggcgt
    17581 aggctttggg ttttgatggt gtgtcttggt acttagtatt atccaatgtg ataccaccac
    17641 ggtacttttt acctgctttt ttgcctgtta atggtacaac caagagtgtg ccgtctgttt
    17701 tatctggcga tgtcagaatg atagcgtagt gcttgccata aaattcgttt ccgccttttt
    17761 gtgtgaaatt gacaaggtag acttctcctt gttggaatgc catggtaact ccttgataaa
    17821 aaaataaggt gcacttagaa aagtacacca gactgtttgt ccttgacgaa caaggcttta
    17881 taaataaata tcgtatcctt gacgaacaag gcttttgact aacctcatta taccaaaata
    17941 agtgaaatag tcaaggatat ttatttctgg tgttgtattt atctctccct atacacttcc
    18001 acgacctcac cgatggttcg gagagtgtgg ggagttttta tgctccgagt tggagttgga
    18061 gttgttcttt gtcagcaaag tttaaaacgc taatgccata ttcttgtaat gccattaaat
    18121 gatcatcttt aatattatta aagccttcat cgcttacgat aatataaagt tgttctttgt
    18181 caccgtattc catttttcga tattcaacgg tatctagcca gcttgctaaa atggtgtcga
    18241 cctgttgttt tgtgatgttg ttgtggactc gtgctatttt tgaataacct ttactcatga
    18301 atacaaaatt aaatcgatgt tcaagccttg attttccagc gattgaaaaa gcagggaaga
    18361 ctgtgtattt ttcgttgtgc atgaaatagt gtttcacatc atctaagaat tgcgatttga
    18421 cattgtttgg gctgagctgg ataaagtcat acacattaat aagtaactgt gtcatatcat
    18481 gaacagcttg tcctagatgt gttcttgtga cgtttctttc aatggcttcg cctactaaat
    18541 caaaaccgtt gtattgcagg agggatttga agagctcttg tcttcgtcct ttttttgtga
    18601 catcaatgcc gtttatcgat aaattccaga cggtgtaact ttcgtcagta attgtaaatg
    18661 ttcttccgtt tgctttaata gaaaatccaa tggcttcacc gaaagggtca acgaatgggg
    18721 tgacaatttc ggtatgacct tctccgatgt cggtaaagac cgcattttct ttgacgaaag
    18781 ccaagtattc ttttttaatg tcgtttgctt tcattgtgac tcctttctct ctaggttcat
    18841 tatagcaaat cttcttggat attgatattg atttcttcag gatttgagat gttggtgaac
    18901 ccaagtaggt cgcttaatgc agtcaaaaaa tcatcagtat ttctaataga ttcgtatggc
    18961 agaggatgtg ctttgtaatg cgttgtttca tcaccttttt ggtaatactc ttccgaagaa
    19021 aagatattta tgcgatttcc ccaaaccttc ttaccatttg cgtttttatg aaatttatta
    19081 tttagattga ttcggataag cgtgtaattg tgttcggttt ctcgaatgtg aaaaactttg
    19141 ctgtttgtac tgtaaaagta atttaaaatg aaccgcttgt cgccttttcc gacaatatcg
    19201 atttttccgt ttgtctgatt gtgtagcgtt ttgttatgtt tttgagcaac aacctttaat
    19261 attttgatta gcttttgtgc ttctgcatct gtgagtagca gttctttcat aaaaatctcc
    19321 ttcctacctc tccctataca catccaccac ttcaccgatg gttcggagtg tgtttttttg
    19381 tttaaggtaa tttccctgct tctttgataa gatttttgtt ttgttgtttt actttgcgtt
    19441 ctaacttttt caagtcttct gctggaggga gttcttctgg tttgataccg cgttgtccaa
    19501 gcatgtttcg gatagttgta ttattcaaaa cgtgttcatc tgtgatggat ttttcgccat
    19561 ggaggttgtt ttcttcaaca ttgtaatttg tcatctcggt cgctagattt tttgctgcga
    19621 tggtcagagt tggtaagaag tcagctagtg gacggttgct tttgacgcca agacgttctt
    19681 tcatttcctg cgtgctatgt cccccaaata gtgccgtgtc gccctttgac cgaatacgtc
    19741 caaatccttt gtcgtcaaca cctctttcat agatgttttg tgataggcgt ttttcagatt
    19801 ctttgagttt gcctcgagct tctgtgcgtt caatatagtg gagtcgttcc tcaatcaatt
    19861 cttgctttct ggtctggact gcaaaatagg attgagcaaa ggcaatctct tctttgttag
    19921 tgtctccgtt tatggcaata aggtaacaag cgtaacgagt aagcatatag tctttaacag
    19981 ggcgttcaga accactgcct aaaggaacca ttttcgtgac cccacgaaaa tggtctgaca
    20041 ccttggtgtc actggtttct acggagttca tcgctctttg gatagctttg tgaaaattct
    20101 cccaacgttc gtaaccaagt aggggcataa ggtcacgggc ataccagtaa tcaatgaatt
    20161 cattttcggt ttgattgact atgctatcga atttttcttt ggttctataa atttttgatt
    20221 gttccatttt ccttcctccc tacctctccc tatacacatc caccacttca ccaatggttc
    20281 ggaagtcggt gtctgctgtg attgggatgt tgtcatagtc tgggttcagg ctgcggagat
    20341 aggcgccttg gtctgtgatg cggagttgtt tgatgtaggc ttctccattg taggcgaata
    20401 ctccgatgtc gccgtcagag aggtccacgg atagtttgac gaatacataa tcgcctgagt
    20461 ggtattctgg ctccatagaa tcgccataga tttgtacaac gaagtcagcg ttcacttcaa
    20521 taggtaattc tacttgctct acttgtacat catttagata ttgtcctgta ccagcagaag
    20581 cgggttggtc gtaatagttg taggtgtagt aggtggcttg cagttcgttt actgtattct
    20641 tactgtatgt tactgtattt tgtttatcta gaagctcgct agaatagcgt agcacgtttt
    20701 tctggttggg ttcggttaat tgcaccacct tgtccgaaat ctgctctatg aggctgttag
    20761 gggctgtggt ggtgatagga ggaaagaagt catcaatact tactttaaaa atatccgtca
    20821 gttcaaacaa agtatcctga cctggttttc tgtcaccttt ttcataccta cttattgttt
    20881 gctttgttgt ttctaaacgt tgagcaaggt cggtctgtgt catacctgcc aattttctga
    20941 agtctttaat tttttgaccg atatatacat ttaattccat aatgtttttt cctttactct
    21001 tttgttatta aatgtagtat aacaaaaaaa tcaccgaaaa ggaaacattt ttgtgttttt
    21061 cgcaaaaaaa gtgttgacaa gtcaccgaat cggtgatata ataaagtcaa ggttaaggaa
    21121 ttaaccgaaa taaaaacagg aggaacggcg aatgatggac atactaaaaa gcctaacacc
    21181 tgacgaactc ggaacagttc tgatagtgct aggcttggta aggcaagctc gtctgtggca
    21241 taaacaagta ctcgaccaca agattgagca agctaaacta aataaaaagt aagagcaagg
    21301 ggcgaaagcc ccaacctctt atttgagtat accaccattc gccagaaaaa gcaatgttgt
    21361 attggttagc aggtttatgg gttttgatgt ttcttggcag gcacgctcac aagcgttatc
    21421 tggataaaaa gcttgaagaa ctcaaaaagg aaaaagaggg aaaggagtga gggttattgc
    21481 aaacattatt gtatggtttg cgtaaagaaa gaaatttaac gcaagaagaa atggctgaca
    21541 aaattggtat ttctgaaaat gcgtatcgtc agaaagaaaa aggacaacga ccttttacac
    21601 aagacgaaat gtttttcttg ggtaagtttt ttaacaaacc tattcaagaa atttttttac
    21661 caagagagtc accaaaacgg taacaaatgc gccagctctg ccatttcccc aatggtcagt
    21721 ctttggtcaa actttataca atacttgtat aaaaagttct tgacttcttc aagaacttta
    21781 actaacaggt ggaaggaggg gtgaccatga cgtctataca tgtgtctttg tcggttgaga
    21841 tgaagaagcg gttggggatg gagtgccagc gtctggggct gtcgatggcg gcttatgtgc
    21901 ggttggtgct ggcggagaag ttgagagagg agtagggcgg tacggctctg ctatttacag
    21961 aaaggagaat gatatgaaca agaaaggtcg accagcaggg gtcaaaaacg aacgtggact
    22021 ttacacaatc gcgataccaa aggaaatcta cgaccaaata gatgatttgg ctatcggtag
    22081 cggtaggtcg cggacggctg ttgcaacctt tgtttttaca aaaggactgg aacatatcca
    22141 aattgtcgag gaaacaatca cacgcaagcg gattgtgggt attgagtagg gaaaggccaa
    22201 ccaaactaga aaggagaagg ggatgaacct tcaaatctta ataacgttgt tactttctac
    22261 cattttttca ttgatttctt tcattgtggt tacaacagca gttgacagga aatctaagaa
    22321 aatgcgtgac gagatgtaca aaagcgtttg ggatgagtat aaaaaaagaa gtggcaagta
    22381 gcctacttct tggagagttt ttggacaaat tgttttttta ggttttgttt ttggttgtaa
    22441 ttttctacct tgtctgcgct agaaaatacg gaattgaggt tgacttctcc cgtcatgact
    22501 ttatagataa gttcattctg ttttaaagct aaatccgttt cagcatctag ttttgatact
    22561 ttctctaatt ctaagagtct gaggtcgtga gattgtttga gagcatctaa ttcttgagag
    22621 tgttgtcgct caatgttttc aagttcgtgc ttatgagata acgatagttt ctccatctcg
    22681 tgttcaaact gaattttaat cgctttaatg tcagaacgat ttttcatcca agaagctcca
    22741 aagctaagca aaccggttat tgcagctggt attgttgcag taccaaaagt gagccaaaat
    22801 tgaaaatttt ccatgagagg tgtctccaat cgtttttatt tttattatag caaattttat
    22861 cacgttagaa aggaatttta tgaatgaaat tataaacatt aatgtgaatg acaatcaaga
    22921 gcctgttgtg tctggtcggc agttgcatga ggctttgggt gttaagacgg catataagga
    22981 ctggttccct agaatgaccg aatacggatt tgtcgaagga caggacttct gctcaaattt
    23041 gagcgaaagt acgggaggtc gtcgagctgt tgaccacatt atcaaactag acatggctaa
    23101 ggaaattgct atgatccaac ggacagaccg tggcaagcag gttcgtcagt actttatcca
    23161 aatagaaaag gacttcaaca gtccagagaa gattatggct cgcgctctgc tattggccga
    23221 caagaaggtc catcagctgg aagcacagat tgaggcggac aagcccaagg tgctatttgc
    23281 agacgctgtg agtgctagtc actcgtctat cttggttgga gacctagcta agcttattag
    23341 ccaaaacggc tttaaaatcg gagcaaatcg cttgtttgcg tggttgcgtg agaatggcta
    23401 tctgattaag cgcaagggca gtgattggaa tatgccaacg cagaagtcta tggaactagg
    23461 tctgtttgaa atcaaagaga cgactatcac acatgctgac ggtcatatct cgattagcaa
    23521 aactgtaaaa gttacaggca aaggtcagca gtattttatc aataaatttt tagctgatga
    23581 tgttgcttga aaaacaaaaa agcctgacgg caatcaggct cttactaaaa ttacttactt
    23641 gaattataac acacgaaagc gaggtttgac aagatggatg acattgcaga aagcctcata
    23701 tcacgcttta tcagtcaact aaaggttagg ctggtagaag tgtttgaggt gtttaactta
    23761 gaactagcaa tgcctttgct actcaacagt aagcagtgca agaagttgct aggtattgca
    23821 aacgagacgg aattccagag ggtgtcacac ttgaaggatt ttccgagaat tgaaaagaaa
    23881 gggtcgcacc cacgatttcc acgagatgcc gtggttgagt ggatgcgtgt gaattggaag
    23941 ttgatatgac agaagcgata tttacattag gaatcttcgc tgtgccgatt ttgacggcgg
    24001 cagtagtgga acagcggaaa gctgagaagg agcgcaagcg cagggagttg gccttgctcc
    24061 gtgagatgga gttggaaaca ctcaagtatg aaacgtgggc acaggctgag cagtattaca
    24121 agacttgcca gaaagagtct gcgagaaata acctgaagcg caatgggcaa caggtagata
    24181 aggagtggaa aagacatgca gaaatggttg gataaacttt tcaaacaaga aaaacctgct
    24241 atcccacgtc cgctttacac actagagcag gaaaatcaaa tattgcatga catggtccgt
    24301 gaaatcgctg aacaacgaaa tgaatatcgt atcgaaaatc agcggctaag ggatgagaat
    24361 gctatgttga ggaggatttt agaaggatga caagtttggt tagattagaa ctaatcgctc
    24421 aatcatacga ccgatttctt cggacaggcg acgctgaaca tcttgaggat gtagaaagga
    24481 ttttggaata tgacgaacat gacagcgatt gataacatgc ttcggcgagt atttattgga
    24541 gtttaggagg aatggttatg gcttatgcag catattgcaa gaaatacaca cgaaaactac
    24601 tctaaaataa ataatcaccc tgctcaaaac agcgaactta gtttacaagc taaagggtta
    24661 ctatttgtgt tgatgtcgaa caaagatact tggcgaccgt acatcgatca actttccaaa
    24721 cgctcaaaga acggtcgaga agctcataga aatgcctttg aagagctaaa agacggcggc
    24781 tatatacgta tctacagaaa aagtctaggg agaggaagag gaattcagaa ctacccctta
    24841 gtttccgaca tccctatcac agatagctat tgggagtact ggaaagaaaa ggttgatgat
    24901 gagttatcca caggtgaatc gtcagagtga atttacaact tacgggtttt acaaagttgt
    24961 atttttcaaa agttgaattt tacaaagttg tatttttcaa aagttgaaaa acccgacact
    25021 aataataact aaataataat aataactaac ttaataataa tctaggggct atcgcccact
    25081 aatgaacaat aagaggctaa agcctctaac taacttaaaa acaaactaat cgttatatat
    25141 aaataatata tatagggatt ttcacaaaag ttatccacag gaggaaaatc ttgtctggca
    25201 acgtgccgcg gcaggagaga aggagaagct actggataca ggtctagctg ataaggcagg
    25261 atacatccgc cttgtcagag agctaggtag aaagtatgtt gcataggagg tacggcatgg
    25321 aaggtactta cccatggttt gattatgacc gtgactatct acaacctgag gaaccaagac
    25381 aggtacacga tcctgatgaa tgggtgtttc gtggcggtca atggatttat gtaggggatg
    25441 cataatgaca gaggaattac tagatacaat ccgacggctg aggtgtgatt atttccacct
    25501 aggccgagag ctgggcgaga ttatcaatga gcaacaggac ttgatacttg ccctgaaacg
    25561 agaaaacaaa cgcttaaagc gtgagaattg gaatttaaaa cagacgaaaa ggagaaagaa
    25621 atgagtaatc ttgcagttat tcaaaaagat attacagatg ccgtgaatgc gaaagtgtcg
    25681 cagatgcaga acgaaggctt ggtagtagca ccaaactacg caccggcaaa tgctttgaag
    25741 tcagcatttt ttgccatgac caacagccca agcgggaact tgcttgaaaa gtgttcgaaa
    25801 gaaagtattg ccaatgcctt gcttgacatg gttgttcaag ggttaagtcc agcaaagacc
    25861 caatgttact tcattccata tgggagtaca ttaaaaatga cacggtccta ctttgggact
    25921 atgaaggttg tcaaacaact gtccaacgtg aaagatattt gggcagaggt ggtctttgaa
    25981 ggggatgtgc ttaaaattcg caatgacaat gggcgcaagg ttctggaaag tcacgaaaca
    26041 gattggacca accaagataa tgcaattatc ggtgcttact gcatcattga aaaagtggac
    26101 ggtgagcgaa ttttgacagt catgaccaag aaagagattg atcgcagctg gcaacagtcg
    26161 aaaaataaat ctgttcaaaa tgcctttcct caagaaatgg caaagcgtac tgttatcaat
    26221 cgtgcagcca agcaattctt caatacgtct gacgatagtg atatcttgat tgaagctgtt
    26281 aatcggacaa ccgaaaatga atttgatgac aatcgtcaaa tcaaagaagc cgagccagtt
    26341 caatcagctg ggcaggatat cctggataag atgaccggaa agactgtcgc tgaagaacct
    26401 gtagaagatg caactatttc cgaagtggaa actgttgaag aagcaggagt ggatatttcc
    26461 aaaatggaaa caaccgagca ggtcattgat gctgaaatcg gcgaaatctt agatgaggag
    26521 gaaccgttct aatgtctgaa gcactatctc tatttgacaa tctggaaagt atggcgccag
    26581 ttccgactgc gacagtatta gattttgact tcgaattcac accagcccaa atcactatcg
    26641 tgggcaagga tttgttggag caggcactta ctggatacgt tgaaaaatac aagaactaca
    26701 ctgtcacggc agaaacgttt gaagacgatg ccaaggtccg agctgagtta aatacactgc
    26761 agaagaaggt caagtcagct gttaaggaga aattggcaga ttacaacaag cccatcgacg
    26821 aggtcaaggc ttgggtggac ggattgttgg aacctattgt caaaatcggc aagtcgattg
    26881 acgaaggtgt gaaggcgttt gaagaacagg aacgacttaa acgtgccaaa accattgagg
    26941 aactattcca ggaagttatt gcaagcacag gaaaagacat tgacatccgt ttgttcagca
    27001 agtattttga tgagttttct aagaaaacat gcttcatggc tgacaatgtt cgcccaaata
    27061 aagccacagt caatatggtt gccagcttgg tagaggaaga agtgaccaag aaggaagaat
    27121 atgaatcagc actaatcaaa attactgaag cagctgccaa agcagacttt ggtccagctc
    27181 cttacgtacg taattttgaa aaaggagcaa gcctggccga catcttgcag gcaattgctg
    27241 atgacaaggc tttggcagat agaacccgcg aggaggttag gcgcaagcaa caactggcaa
    27301 aacggattga agagatgact gccatcgcag aaagcaaggg actagatccg aaaaagtatg
    27361 ctgatatgct cgaatcaggg gtatctgcac tagccgtcca cgaagagctt gtcaatgacg
    27421 caagaaaaca gcaagaagag caggaccgaa tggaacagga atttctagct cagcatggag
    27481 ctgtttgcgg aaatgttcaa aatcgcccga attctgatga aattcaacga gaaaatgtat
    27541 cagagggtaa atatacctcc gaacaaaaaa acgcgtcaga agacaaaata gagctgaata
    27601 agaaggcggt caaattgcaa ggtgatttca gaatcacttt cccagatgga gagacagcta
    27661 agttattcgg tggtaagggt ggtttgtatg aacaacatgg gatagttgtt gagaaattag
    27721 gagaatggac aaaaatcaat ggctaaacta actgaagaga attactacca agaccgccaa
    27781 tggctttcaa attctcgctt taaggcttac atggactgcg aagcgaaagc taaagccatt
    27841 gatgataagg agtgggcgga taatcgtgat gacacggctt tgcttgttgg aaattacgtt
    27901 catacctact ttgaatccga agaagctcac gccaattttg ttgacgccaa caaaactagg
    27961 atgatttcga gccgtggagc gactaagggc gagctgaaga aagagttcca agtcgcccag
    28021 aacatgattg atgctctgaa ggatgacaag gatttcttgc ctctctatca cggcaaccca
    28081 ggcgacgatg tccgcaagga aatgatttta gaaggcgaaa tcttcggtat caaggtcaag
    28141 ggtaaggtgg atagtatcaa cttgactgaa ggctattttg tagacctgaa aactatgaag
    28201 accatctgcg gtcttgaatg gtccgatgtg gaacgaaaga aaatccatgg agctgctgct
    28261 aacattttag gtttccgcta cgatgtccag ctggggctgt atcaggaatt gttgcgacaa
    28321 atgggctatc caaatttcgt tccgttcgtc gtcgcagtca gtaaggaaga cgtgcctgac
    28381 aaagccgtta tcacactttc gcagtatcgc ttggatgaag gcctgcaatt cttcgaaaat
    28441 aacgtcgaac atgttgcggg tattattgca ggcgaaatca agccgaaagg ttgtgggaat
    28501 tgtgactatt gtcgcagtaa gcgaaccctg gaccgtgcca tcaatttaga tgatttgatt
    28561 gcgggggtat tttgattgag gagaaaaaag aaatgagtac aataaaaaaa tggcagacaa
    28621 tgcctaccaa aaatgatgaa actaaattga atgacctagc taaaaagctt ggtgtatagc
    28681 cgttaaatag ccgtagtaaa aaatgtcctt tgaaaactta ataaaaacac ttgacaaatt
    28741 tgtctgacaa gaattataat aattgttgtc agacagaaag gtggtgaacc atgactgaca
    28801 atagaaaaac agtaggtcgt cccaaagctg aaaagcctca agacaaacgt gtaactgtga
    28861 gaatgacaca agaaactttc gagaaatttg aaagttattg cgaaactcaa aaagtcacaa
    28921 agtcagaagc aattcgcaga gcggttgagt tactcaaata aaaaaacaac ttagaacccc
    28981 taacgccaat cagacggttc taagctgtcg ccccgaagtg taaactccgt gaaatctatt
    29041 atatcacgag tggacacttc ttttcaagat acacaaaagg agtgttttta ttatggctaa
    29101 aaatcaagaa cttatcaatc atatccacct agcgttcaaa gaaaaaggac aatcagaaca
    29161 acttatgcag tgggataatc tcatctcgat tgttatcaat cgtgactttg acgcgcagaa
    29221 agcaaataca ttcttcgagg ctcacgcttg tttgttgtct gtatttgcta atgatttgat
    29281 tgaatttgtg ttagatatgt ttacggagga tgtagaagat gtctaaggaa atctggaaag
    29341 atgttattgg ttacgaaggt agttaccaag tatctaattt aggcagagtg aagtctgtgt
    29401 cgagagaaat tattaataga ggggtaagac agaaaattaa agaaagaata ctcaaaccgt
    29461 gcaataaagg agggtactta tttgtgactt tgagccagtt cggtaagctt aagacatttg
    29521 ccgtacatcg ccttgtcgct attacatttc tcccaaaccc agaaaacaaa ccacaagtta
    29581 accatttaga cgaaaatccg ctgaacaata gattaacaaa tttggcttgg gcaactgcaa
    29641 acgaaaactg taattacgga aatcgaaaca aaagagtttt caaggtcgga gtagtcaaac
    29701 ctagatctgt ttccatgtat tcgatagagg gtgaattatt acatattttt tcttcaattg
    29761 cagaagcgtc gaatttttta ggaaaatcac ctagttgtat ttctgcttgt gcaggaggca
    29821 aaagaaaaaa agcccacggc tatatttgga aatacacgta atgaataaca agctgggcat
    29881 ccttgtaaaa ctgcaaccta gaaagcgtgt caattggaaa caatcagttg acagttggac
    29941 gacagcgact gcccgtattt agccaaactc acacaatggc agtcgctatt ttttgagaat
    30001 atgatagctc atgaaaaaga gtacgctctc tacaaaggtg ataagctact agcaatcgga
    30061 acagcaaaag aattagctga acaattcggc gtgaaggtgt caacgataca cttttacaag
    30121 tcaccggcct atatcaagag aacaaacgag acgaaagcga ggcggttggt tgaagtttga
    30181 atttatttta tctaacacta agcgcaaaaa agagatgctg aatgctaatg acagaatgaa
    30241 ctgggcacag aaggcaaaaa ttaccaccta tcttcgccgt atagcgtttt ttaaagtctc
    30301 cgagggtaaa tataccaccc acacaaaaaa gcgcccctgc ggacttttgg tgacggttta
    30361 cgcaccaact aaaagacgga tggacccgcc gaacttttac ccgactgtga aagctctagt
    30421 cgacggtatg acagatgctg gtatctggac agatgataat cacaaggtta ttaaaaagct
    30481 gtcatttgtc tatggtggtt tgagtaaaga aaaagggcat tataagttta tatttgatat
    30541 tgatggggta aaggatgatt agtggttttt cgcttatttg caagaaaaca agacaagtta
    30601 ggtcaatcca aggggcattg atgtttgata caccacatga tgcccaaaaa gcacttaaaa
    30661 aggattgcaa gcgttgtggt gaaaattatg ataaggaact ggaacgctat gatattgtgg
    30721 attttatggc aacacagttt gaggctggtg ttttagtaaa tggtatgcga tattctttgc
    30781 atcgaatgaa attaagcaaa gaacatctca aatctttgtc agatggtgaa aaactcatgt
    30841 ttagcaatca tgacattaaa aacctaataa ctttggaggt caaagatggt taatgttctt
    30901 ttagatgtaa ctggttttga taaagataaa gataaggctt ttaagttatc gctaaatgtt
    30961 gaaaaaatta tagccattgc agaagatact tttgttgtat ttgatgatgt tgcaggtgaa
    31021 tatgttgagc acgttgggtg tgagataact gttgatggaa gtctttgtta taaaattctg
    31081 gagccttatc aaaaggtaaa agataaattt gtgaggtgct aaatgaccaa aaagaaaata
    31141 gagcgcttat cagtcataca ccgcagggaa atcacatggc tcaagtggta ttttttgagg
    31201 gataagaaaa atcctaaaaa gacggtacta gagcagatga tacatgatag tttcatgaag
    31261 aatgacacag accaagcaac atttttggtc aatctaaagc tagtgacatc tgaacatgtg
    31321 gaaaattcag aagaaaagct gattgagact ataaaagagg tctatgttta tgaaaatatc
    31381 aatgtcattg gagcgtgcca aaagatttta ttcctaagcc ccagcccagc ctatacccac
    31441 ctcaataaat ggtttgatgc ctatttttac gccacctata aatacctccc tttggaaaga
    31501 taacagtaaa aattccctag cctatgtatc tataatcaag gtacataggc ttttttgcta
    31561 ggaggagaaa catggatata ttgaaaccac atcacaggca aaatacattg agccagtaca
    31621 acctactaga ttatgatgcc acgcgcacag ctggcaagta taatatccct actcttgagc
    31681 cagtagacca tgtgccaacc aaactacaag ggtttaacta tgttttgaac aagcctaact
    31741 attcggcagg tgtccacttc tttttggatg attaccagtt tgaacgagtt tggaaacgcc
    31801 cagattttta tattgataaa ctagctgatt ttgattgtgt gcttacacca gatttcagcc
    31861 tctatactga tatgccactt gcaatgcagg tatggaatgt gtatcgttca agattgattg
    31921 gtcagatgat gcagaactgg ggctatacgg ttattcccac ggtgtcctgg tctcacaaag
    31981 acagctatga gttttgtttt gatggcatac ctacaaaaag cattgttgca gttagtacaa
    32041 ttggtattaa aaaaagcaaa gaccgtatca aggtatggaa agatggcatg gatgccatga
    32101 ttgataaatt gaaaccaaag aaaattttgg tttatggcgg tcacattgat tatgactata
    32161 acggtatcga ggtctattat tttgctaacg atacaacaga aaggatggac acatgggagg
    32221 cagaggggca agctctggaa tgagcgaaaa gcaaaagaaa tatggtacag agtatagcac
    32281 ggtgcatcaa tcgggtaata taaaatacgt tacccaaaat gcaagtgggt cgcaaaaaac
    32341 tcccatggaa accatgacca aggggcgtgt gtatgtgctt attgacaaaa acaagaacgc
    32401 tcctaaaagc attgtttatt ttgataagga taacaagcgg agcaaacagg ttgacctaga
    32461 ccataagcat cagaaaatga gacctcacac gcatcatggg tataaccatg ctgaatatga
    32521 aatcagcaaa aaaggagcaa gcaaactgac cacaacagag gcaaagctag ttgaaaaagt
    32581 caatacagag tggtataatt atcttaagaa acgtagggag tagtatatag ggattacgcc
    32641 ttgatggagg agattccggt tcgattccgg gctactacgt tacttcctag cccctaattg
    32701 gggcttttta ttttatatcc aaaaaatagc gtaaaacatc cccttttcaa ccttataaaa
    32761 tgaaatcatg agtagtaata cttgtgattt ttttgttaga aaggagagag aatgaatgac
    32821 aagcaaattc gctttgcgga tgagtttatc aaactcggaa acgccacaca ggcagcctta
    32881 aatgcagggt attcggaaaa gacatcttac agtcaaggac agcgattgtt gaaaaatgtt
    32941 gaagtaaaag agtacatcaa caaacagatg caagagctac ataagagtaa tatcatgcag
    33001 gcagaagaag ccctatccat cctatctgac attgctagag gtaagcgaga tgaggaggtg
    33061 ttgatactta acccaacaac agggaaagtt gagagacaca gcaagaaaac tgataatgca
    33121 acagttatta aagccattac tgaaatctta aaacgctacc caaccgcaaa acaagctgaa
    33181 aaactagagc ttgagattga gaaattaaaa tcacaattga cagaggtaca gattgaagat
    33241 gagactataa caatcgctga taagtgggag gttgaagatg aaagtagtta atatacagaa
    33301 taatgtcaat ccacacttta agagtgtttg ggtatctaaa aaaccttaca acattctaaa
    33361 aggtgggcgt aactctttta agtcatctgt tattgcattg aaattggttt atatgatggt
    33421 tagatacatc ataagagggg aacaagccaa tgtggttgtt atccgaaagg ttgctaatac
    33481 tatccgtgat agtgtgttta ataaaatcca atgggcatta actttatttg gattgatggg
    33541 gcgttttaga gcaacggtaa gcccatttaa gataactcat aaaaaaacag gctcaacatt
    33601 ttacttttat ggacaagatg actttcagaa attgaaatca aatgatattg gtaaaattat
    33661 tgctgtttgg tatgaggagg cggcagagtt tggcagtcaa gaggattttg accaatcaaa
    33721 cgttactttc atgcgtcaga agcacccact tgcaccattt gtacagttct tttggagtta
    33781 taacccgcct agaaacccct ataactggat aaatgaatgg tttgagggga tgaagttaca
    33841 ggatgattat ttgtgtcact ctagcactta ccttgatgat gttttaggat ttgtaacccc
    33901 gcagatgtta gccgatatag agcgcattaa agaaaacgat tatgactatt ataggtattt
    33961 atatttaggc gaggctgtcg gtcttggtaa taacgtttat aacatgagta catttcaccc
    34021 attagatgcc ttgccaagcg atgataggct tattggtata tcctttgcac ttgatggagg
    34081 acaccaacaa tcagccaccg cttgttgtgc ttttggtatc acagctaaag gcaaggtaat
    34141 cttacttgat acttggtact atagcccagc aggtcaagca gtaaagaaag cacctagcca
    34201 gttatcacaa gacatcaata gctttataca atccgttata agccagtata aagttcctat
    34261 cttgcagtac acaatagata gtgcagaggg tgctttgcgt aaccaaatgt accttgactt
    34321 tgccattagg tggcatccag tagcaaaatt aaagaaagtg acaatgattg acacattcca
    34381 atcattattg gcagaggggc gcttttatta tctcaataca gagaataata aggtatttat
    34441 cgaagagcac aagatgtaca gatgggatga caagacaata cagacagata acccaaatgt
    34501 tatcaaagac gatgaccata catgcgatgt ggcacagtat tttgtattag ataatgcaaa
    34561 gatacttggt ttgcgtgttg gtaattcata aggagggcaa acatgagcct aatacagaaa
    34621 gtaaaggact ttttcaaccg tgggaggtat aacatgacga catcaaacct aaacagcatc
    34681 ctagaccatc caaaagtagc tgtaacacaa tctgaattta gacggataca gcataaccta
    34741 gcttactatc aatctaaatt tgatgatatt gagtatacga atactgatgg tgatagaaaa
    34801 cgtaggaaga tgcagcactt acctattgct agaacagcag ctaaaaagat tgccagcctt
    34861 gtttataatg agcaggcaga aatttcagca aaagatacta cactcaatgg ctttttgcaa
    34921 gatatgcttt ctaatgaccg ctttaataag aactttgagc gatatttaga gagtgctttg
    34981 gcacttggtg gacttgctat gcgcccttac gttgatggtg ataaaatccg tgtggcattt
    35041 atccaagcac cagttttttt gccattacaa agcaatacgc aggatgtatc gagtgctgct
    35101 atcttaacca agactatcaa aaccgaaaat agaaagaatg tgtattatac acttgttgaa
    35161 tttcatgaat gggtaacaaa agatggtcag gaagtaggca gtacgaaaga taaaagccta
    35221 taccgtatca ccaacgagct atataaatca acatcagata gcacattagg ggagcgtgtg
    35281 aacttgagtg agttataccc agacttacaa ccagtaaccc caatacaagg tttgtcacgc
    35341 ccgttgttta cttaccttaa aacacctgga atgaacaata aagacatcaa tagtccattg
    35401 gggctatcca tttttgataa cgctaaaact accattgatt tcatcaatcg tacctatgat
    35461 gaatttatgt gggagattaa gatggggcag aggcgcgtta ttgtgccaga gcaaatgaca
    35521 caattaacag tgcaggatag ccgtggagag attacgttca agcgtcgctt tgatgttgaa
    35581 caaaatgtgt atatgcaaat aggggcaggc aatatggata gcggggggat tgttgacctc
    35641 acaacaccta tccgctcaag tgattacatt tcagctattt cagaggggct taaattgttt
    35701 gaaatgcaga taggggtatc tagtggcatg tttacctttg atggtcaagg agttaagaca
    35761 gcaacagaga ttgtaagtga aaactctgac acatatcaaa tgcgtaatag cattgtggca
    35821 cttgttgagc aatctataaa agaactttgt gtgtctatgt gtgaactcgg caaggctgtt
    35881 gggttgtata atggtaaaat cccagaactt gatgacattt ctgtaaatct tgacgatggc
    35941 gtgtttactg atagacatgc agagcttgat tactggatga aaatggtagc tgctggattt
    36001 gccacacaga aacgaggtat tgcgaaaacc ctaggcatca cagaagatga ggcagccaaa
    36061 gaacttgctg aaatcaatgg agaattgcca ccagagagcg atgcagagct cgctttgtat
    36121 ggtaaaggtc aacaaaacac agtaggaaac agtaaagata cagtagaggg agaagatgat
    36181 ggcgaagtat aagaaaaaac cagttgtaat tgaggctatt agatttacag gctcaaatta
    36241 tgaagaaatt agagaattta ttggaaaaaa tactttatgc tctgatttaa gtatcgtaat
    36301 accaacactt gagggagata tagtagccca aaagggtgat tatattatta aaggagtgca
    36361 tggcgagttt tacccatgca aaccagatat ttttaatgaa acctatgagg tagttagcga
    36421 ggcttaatca tgagcgatac aaagaaacgc ccaactatca atgaccagca attcagctta
    36481 cagatgcagg gggtatctga tatttacgcc cagatgcaaa cagagttgtt tgatagtatg
    36541 attgaacgac tgataaggcg tggcagtgca gacctagcag aaaatcccta tatttggcaa
    36601 ctggaaaagc tgaatgatat gtatatgcta aatgaggaca atctaaaaat cattgttgag
    36661 cgtacaggca ttgcagagga gcttttacgg gaggttatag ctaatgaggg gctaaaggtt
    36721 tataaggaca ccaaagaaca gctagaggag gatatgggca agtttccaga tggcaaaatc
    36781 agaaacggtg tgacagatgc cctggaggct tacacacagc aagccattag tgacctaaac
    36841 cttatcaata ccacgttgcc acaaagcgtg caaactgtct ttaagtcagt tgtcgagcag
    36901 actgtggcac aggtagtatc aggcactaaa acaagtgaac gggcattaca tgataccatc
    36961 atgaactggc agaaaaagaa ctttacaggc tttacggata gtgctggcag ggaatggcgg
    37021 gcagacagct atgctagggc ggtcattaaa acaaccactt tcaaggtgta caatgacatg
    37081 cggacaagac cagcagaaga acttggtata gacacctatt actactctat caagcggaca
    37141 gccagaccat cttgtagccc gttgcagggc aagattgtta ccaaagaggg gagcgagcgg
    37201 aaagaacacg gcataaccat acactctttg caagattacg gctatggcac ggctggtggt
    37261 tgccttggcg tccattgtgg gcattacctt acgcctttta tcgttggagt aaacgagttg
    37321 ccagacctac cagacttttt gcaagacctc acaccagagc aggcagagga aaatgcacgc
    37381 atcgaggcaa agcaaagagc cttagaaaga gccgttagaa accacaagga gcggttgcac
    37441 tatgctacta ccatgaatga tgatgacctt atccaagctg aaaggctcaa ggtcagaatg
    37501 tatcaaggta aaatcaaggc tcttgttgat gagcatgaat ttttatcacg agattacaca
    37561 agagagaaga tttacacata gtttgggtgt tgccattggc aatgcccttt ttagataggc
    37621 tgaaaacagt aaaaaatccc attccaaaga aagtaaactg aaaaagtaaa taatattttg
    37681 ctttttggtg ggagttgtcc acctaaaaaa gaactaagga ggtacaaatg gcatttacaa
    37741 cagaggaact gctcaaactt ggattgacag aggaacaggc taaagatgta tttgctttgc
    37801 atggcaaaga cttaaatgct aataaatcag ccttggaaac tatcacacaa gaacgagata
    37861 gccttaaatc acagttgcaa aacacagagg cacagcttga aacgttgaaa gcagatgcaa
    37921 atacaagtgc tgaacagaaa gaagctattg ataaattgcg agctgaatat gacaaataca
    37981 aagcggatgc agctgctgaa cttgcacaaa ctcaaaaggt caatgctatc aatcttgcat
    38041 tgaaagatac caccgcacac aatccatcaa ccttgatgaa gttcattgat gttgatgcca
    38101 tcgagcttga cgaaaatggc accccaaaac tagatgacat actcaaaggt ctaaaggaaa
    38161 gtgaccctta tcttttcaaa gcagatgatg acaaacctaa cccaaacatc gtaccgccag
    38221 gtaatccagt agctaatgac acaggtaata ctgacccatt ccaagcaatc atggataact
    38281 acggcaagta aaagaaagga gattacacat aatggcaggt aatcaaaaca atccagtccg
    38341 ccgctatgaa aagcagtatg cgggcatgct gcaaacagta tttggtattc gtaaagcatt
    38401 tgccaaaact ctagccccta ttcaaatttt ggatggtgtg caagaaaatg ccaaagcatt
    38461 ctctgttaaa acaaacgcaa ctccagttgt catcggtacg tattcaacag gtgcaaatga
    38521 tggtggattt ggtgatggct ctggagcaaa atcacgcttt ggaaatatga cggaagtcaa
    38581 atatgacaac gcagatgttg agtatgatta tactctttca attcatgagg ggcttgaccg
    38641 ttacacagtc aacaacgatt tggatgctgc tgttgctgac cgtctgaaat tgcaatcaga
    38701 ggaacagaca cgccaaatga acactaaaat tggtaaattc ctggcggaaa acgctggtaa
    38761 aacagaggca cttgcagact ttgaagaaaa gacaatcaaa tccctattca acaagttgga
    38821 tgcctactat acggatttag aagtggctgt ggacattact gtttatcttc ggtctgaatt
    38881 gcatagtgca attgttgata tggcatcaaa cacttctgct aaaggctcaa gcgtttctat
    38941 tgatacaaat ggtttgctca aatacaagga ctttatcctt gagaaaacac cttcaaaata
    39001 tttgccaacg ggtactttag cggtgttttc gccagatggc attgtcattc catttgttgg
    39061 tatttcaaca gcccgtacta tcgaggcgac agattttgat ggtgtgaaat tacaagcagc
    39121 tgctaaaggt ggtacttaca tcttggagga caacaagaaa gcggttgtta aagtcacagg
    39181 aacagttgtc taggaggtaa acaatggcac tttataaagc aacaaaaaac ctagtttttc
    39241 aatcactagg taaaggcgtg attgtggatg agattattga ccttgagcca gcttatgtcg
    39301 aaaaggtcaa taaagacctc aagctatcat ttccagatgt tgctgctgta cttgtggcag
    39361 ttgatgagac tgttgaggat gcagacggca caacagatga tacaccatcg gaaactgtcg
    39421 atgagactgt tgaggatgaa aagcctaaaa agtcaacacg caaaaagaaa attgatactg
    39481 acacagtagc agatgttgca gctgaatagt aaggggtggc aacaccccct actttgtaag
    39541 ggaggttact atgccttatt taactaaaga tgagtttgtt aaagatttag gctttgatga
    39601 tgtgactaac tttggcaagc tggctaaaag agcagaggtt gctatcaatc tctatactca
    39661 aggtatatac caacagtata ttgactttga taaagaggtt gagtatcgca aatctgctgt
    39721 caagttggca atggcatttc agattgctta tttggatagc tctggcatca tgacagccga
    39781 tgaaaaacaa gccatgtcaa gcgtttctat tggtcgtact aaaattgatt atggcagtca
    39841 acaccgtatt tcagcaggtc aacaattcaa cctttgcttg gatgctgaaa acgccctaaa
    39901 acaagcggga tttagcttaa ttgcgggagt tgattatgat agataaacgc ttactacaag
    39961 atgttgttac agtccgtaag gttgagggca aagatgattt tggagatgta acgtattctg
    40021 acccattgga tattaagcca gtaaggtttg ataggtcggt ggctgttaca ggtactaaca
    40081 actctaaaac aaggcagaaa gtgggtactg tttatattta ccctaagttt gcaagtgtga
    40141 cagttgatga tagttggctg ggtgcaattg taaatgatgg ggcgcgtgat taccttgtaa
    40201 cagggtatca acccaattac cttaatggca atattttcag ttatgaggtt gaggttactt
    40261 aatggcagat gtcagagtaa cagttgatct tgctggggtt gagaaaaaag tatctcccca
    40321 atccatgcaa cgtggcaaga ttgccgctgg tagcgaggct ttgcttatta tggatagttc
    40381 aatacctctt agggcaggtg gaggggcatt aagagcctct gggcgtgtag aacctaatgg
    40441 agatgcgagc tataacacgg tttatgctag agcacaattt cacggcacta atgggattgt
    40501 tgtatttaga aaatacacaa ccgctggtac tggaaagcga tgggataagc cattaaaagc
    40561 aaacatagaa agactgagaa aggcagctat taaaggaatg ggtattagat gatgcagaac
    40621 aacaaaaact ttcagcaggt cttactagac cacattaaca acatcgaaca cataccacta
    40681 aaggcacgtc ttgattattt caaggatgat aaagatgatt tggtcatcaa tgccttatct
    40741 ggcggtgtca ttgataaaga gtacatggat ggtactagag aggtatcact accatttgaa
    40801 attgcgatta agtgcaagga aaacaaaaag gctattgata ctatttggct catcaacggt
    40861 gatttgtcat cacttgatat agacctacct agtacagata actcttatac attcttgtca
    40921 ttaggcgtgg ataagccagg catcaatggt aaggatgaac aaggttactt cgtttattcg
    40981 atgcatgtaa ccgcaaaact agaaataaca ggaggataaa aaatggtacg aaataaaaac
    41041 gccaagcgca aacatgagat tgcgccattt gacccccaaa atccaactaa agtgccagat
    41101 gagagtgctt ggaaacgact agccaagtac attgaaacta tcgatgatga aacagatgaa
    41161 gaaacggatg atactggtta ctatgacggt gatgggactc cagaagaagc cgtcattagt
    41221 gtctctggcg catattctgt ttctgggtca tacgacccag atgatgccgc tcaagctatg
    41281 attgcagcaa tgaagtatga gactggcgat gcacgccgtg tatggcatcg tgtgacagag
    41341 tcaaatgaga aaaaaactta tacccaagtc gcaaatgttt ccgaaatcaa agctggttct
    41401 ggagatgcaa caagctacga agagtttggt tgcacactga agtggattaa gaaaccaatt
    41461 attgcaggtg tgggtggata agaatttttg gaggaaatag aacatggcaa atacttttaa
    41521 tctgttagga aatgacgaca gcattacttt caaccttggt gattttacat tgacgttcac
    41581 cccaactgat gcaaagtcaa aagcagtatc tgaaaaggct gttgagctga aagataaggc
    41641 tgacaccatc aaagagggta acgagtggga aaatcgggaa agtatcaagg gtctacttga
    41701 tgatttcttt agcactatgt ttgatgcaga tgccccaggt aagatctacc aggcagcagg
    41761 agaaaatact tggaattatc ttaaagtatt cttgcagatg gcagaagcca ttatggaaac
    41821 gaagaaaaaa caagaaaacg atgaaacatt taagaagtat cttgctgaat aatgtttgat
    41881 atttccaaaa aaatggatga caagctggta ctcaacaaca cagagtatca gctttttcta
    41941 tcgtttgaca gggtgctttg gtgtttcgat atgtggggca aggcgcatat acccccagaa
    42001 ttaaaaccta aactggcact agccaaatta accgataatg agagttttaa ggatatggac
    42061 acgatggagg ctatggctat ctatcaagaa gtgtttgaaa agcatatcaa ggtaattagg
    42121 gctgctgacc atgttgttag gtacgacatt gagggcaatg tactaccaaa aagacccaaa
    42181 gaagacccag acaacgaaga aaaacccttg ttttcaatca aatatgatgg cgagtacatt
    42241 ttttcatcgt ttatgcaagc ctataacatt gatttgattg aggaacaagg taagctacat
    42301 tggaagaaat tcaatgcttt actatctgga ttgccagatg gcacaaagtt tgttgaagtg
    42361 atgaaaatta gagcttggaa accctcaaaa agggatagca ctaaggaaat acaaaggatg
    42421 cgtgaattgc aagaagaata cgcattaccc gatatttaac agggaaagga ggtaacacat
    42481 ggcagatgga aaagtaacca tttcggttga cctggacggc aagcaagctc aggggggcat
    42541 tgaaaagtta aaaggtgcat taggcgggct tggctcaaca ttcaaatcaa tgttgggagc
    42601 aaaccttgtc ggtggtgcat tgatgagtag ttttaatgcc attgctggtt cggtcaaaag
    42661 tgttttctca tcagctattg atgagggagc gaaactgcaa cagtctcttg gaggtatcga
    42721 tactcttttt aagaactcag cagataccgt aaagaactat gcaagcaatg cctataagac
    42781 agcagggctt tcagcaaatg agtatatgga aaacgtaacc tcgttctctg ctagtttgat
    42841 ttcttcttta ggcggagata cggcggcagc tgctgaattg gcaaacagag ccatgacaga
    42901 tatgtccgat aacgcaaata agatggggac tgatatgcag gctattactg gcacatatca
    42961 atctttggct cgtggtaact acgctatgct agacaacttg aaacttggtt atggtggcac
    43021 taaggcagaa atggaaaggc ttattaagga tgcctcctcg tataaagata ttcaagatga
    43081 gctaggtata tctgtagagg aagggaatat gtcctttgct aacatggtta aggcgatttc
    43141 tgttgtgcag aaaaaacttg atattacagg gacaacaatg aaagaagcct ctacaacatt
    43201 ttctggctct ttgtcaatga tgaaaggggc ttttaacgat ttcctcggaa acttgacaac
    43261 aggcggtgat attactaagc cattgcaagc attggcagaa agtgcggcga cctttttgtt
    43321 tggtaatttt atcccaatga ttgctaatgt ttttaaaggt ctcccaacgg ctctatctac
    43381 ctttatagcc agcgctaagc ctgcacttgt taacggttta aaaggtctat taccagaaga
    43441 tgctttaaat agcgttacaa aggtcattga cctaatcact ttttcgatta gtgacatggt
    43501 gatggctttt caagactttt accaaggttt cgagaaaaca ggagctatca aggcgttatc
    43561 aagtgccttg tcagagttac taacggctgg actagacttg tcagaaaagt tatctggcat
    43621 tatcccatgg gaaaccatcg gggcagccgc tgggcatgtt gttaagttta tcgctcaaat
    43681 catccaggcg gttgctaaat tctcccaatc catgagtggc gacacttggc gtggtgttgt
    43741 gactggtatc ggtggtgcat tggtagcctt taaggctttt agctttttaa aatctttcaa
    43801 tccattcagt tttttcaaaa aaggagcaga agaggctata gcgggtacaa caggcagtgt
    43861 caagagtgcc aaaagtacca tttcacaggt tttcagcggc atctctaatg tcattaagac
    43921 cacaggaaat gccctaaagt ccactttcca aggtatagga aagatgtttc aagggttggg
    43981 taaaaccttt gaaggagtcg gaaaagggat aggagcagct ttaaaaggtt tgatgcaagg
    44041 attgagaggt ctaaacccag caactctact atcatttggc gcagcaattg gtatagcagc
    44101 agttggcatt ggtgcgggca tagctatcat tgcagcagga tttgccctat tagcaagtca
    44161 aggagagggt atagctatca tcgttgaggc ggttggtaca gcctttggca catttgccag
    44221 catggtaatt ggtgcatttg cagaggcgat tgtaaccgtg gcaggtgtac tgccaatcat
    44281 tgcccaatca tttaacatga tgactccagc aatcatggca gttggtgcgg caatcagtat
    44341 gattattaga gcctttagct cacttgctcc tgtgattacg gcactaggta cagcgattag
    44401 cgagattgta acagcaatca caacaggcgt ggcggatatt gctacggcag ttacaccgat
    44461 ggttgaaatc ctttcaagcg cctttgtaca ggtcgtaaca gttgtatcac aagctattgt
    44521 ggagattata caggctcttg ccccattcat tccagcagtt agtgagatgg tgcaggcggt
    44581 agcgccagtg ttgcaagcat tggttgaggc attcaacaat ctcattagtc agattagccc
    44641 aatcattgac agtatcgcaa acctattcaa aacacttggc gaacaaatca catctatctt
    44701 agatagcgcc agtggggttg tggagtcatt tggctctgcc attcgtaatg tcttggttgg
    44761 cattgcaggt atctttgaca gtattggcaa tgcagctcta aatgctggta aaggtttcaa
    44821 tcagttggcg caggggatag aacgtataac caaacttaat ctgcttgata tgggggcaag
    44881 tctagctgcg gttgccgctg gattaggggc tatcggagca acatctggtg gacttactag
    44941 cgctggttcg tctttgagtg ttattgttca aggtttgatg acagtgcaat cagtaagtgc
    45001 tattgcttct agttctttgg catctttagg aactgttgcg gttagcgcaa tgtctatgct
    45061 cgtcgcagga gttcaactgg cggtatcagg tacaaaaagt ggtatgaacc agatagtaaa
    45121 tgcaataagt ttatccgcta atcagatgat acaggctggt aaacaagcag gggacggtac
    45181 aaccaaaggt gttgttacag gtattcggtc tggaattggt caagctggcg gtgctatgca
    45241 tgctatgatg attgcaatcc gtgacacagg tatgcagggc gtgagttcta tgcgtgtggt
    45301 aggtagcatg ataagtcaag gtttagcgca ggggatgatg tcatctatcg gttctgttac
    45361 tgctgctgca aatgcattgg tagcacaagc agagcgagca gcaaaggcta aagcacaaat
    45421 ccactcacca tccagacttt tccgtgatgc aattggtcgt ttcttgccga tgggtgttgc
    45481 tgttggtatc gagaaaaata ctaagtatgt tgacaaagct atggatggta tgtatgagca
    45541 tatcaacgct tttaactaca aagcagagga tgtgataggt gtcggtaaaa cgaaactatc
    45601 taaggttgta cagatcaaat cagaccttga gagtgctatt aaagctaccg ttgaagttgc
    45661 caaagaaaag agtaatgaac ttttggcgaa agctctagat gttgcagaaa aagccgtaga
    45721 acgaccagca gaaatgcgac ttgatgacgg cactctggtt gcaaaaacag gggataagtt
    45781 tagcagctat caatccgagc aactacgcag agagaacaga atgaaaggaa taatcacatg
    45841 acagcaataa tgacattcaa cggtgttgat ttgtcacgct acttgcgaat tacagagatt
    45901 atccgcccaa ttggtaacaa aaggagcgta acaactgatg acgctccttc tttgggggtt
    45961 aatatccagc aagtgaaacg tggagctaaa gaacacatca tcaaatttga catgaaaaat
    46021 agcgatgtaa tgattttaga gcaactaaaa catgagttag ctggtgtgct tgatgtgaca
    46081 gagccagtaa aaattgttta tggagatgag ccagataaat attatatggg aatacctgtg
    46141 gatgatatta caccagacaa cctaacacgt tggtttcagc gttcagaatt taagttactc
    46201 ataccagatg gcgttgccca cagctcaact tatcgtaagt ttgatagtgc aactgtaaca
    46261 ggcgaaaaga tggtgcttaa cttacgaaat gatggaaatg tgccagcaaa tccaatcatt
    46321 actgttaagc ataatgcaga aaatggttat attggcattg ttaatagtac cggtgcaatt
    46381 gagctaggta atatcgagga ggaagatagt cagacttacc aacagtcaga aatcttattt
    46441 gattatgttt ccaataatgg aattatcaag ggttttgctg atgggaagaa aaatgtggct
    46501 attttaaatg acaactcaca atcgtttgat acgaatttgt acgtaaaaaa tgaatttggt
    46561 cgcccgcatt tagctttggg taatcgtgga gctggtgtcg gacctaacca tgctggttcg
    46621 attagttggg atataccatt ggatagtgct ggtcaatctg gagcgctcaa tgaatacatt
    46681 tggtggagac agatattttg ggctggtgca gtcaatcaat acggatttat taaagttatg
    46741 gtatctgacg aaaaaggaca atttttatat ggagttgaga catataaacg ctctattggt
    46801 gtagaaagcg agtataactt tttagcatca gatggaaagg gtggatttaa tgtattaaaa
    46861 cgttggactt ttaatgctac tcatcttgat agcgataacc cgtttaatgc cgaaagaggg
    46921 tggtcagata taaaacgaaa tgatgaccaa gtgcaggtct tctggtgggg gagttaccca
    46981 gtatttacca taccagaaat aaaaggtaaa aagtccgcta agatacatgt ttcaataggt
    47041 gcagtaggag gaaaaccagc catccatcat gcttatattg atagtatcgt ttaccgtaaa
    47101 gattttgtac agggaacaaa ggatattcct aatcgctata tgatagggtc taatgtcgtt
    47161 gttaattgtg agaacgatag tgtaactgtt gatggtttgt ctaaatcttc tgatttagtt
    47221 gatgggtcta gttggagact gtctatacca aaaggagagt cacagttaga aatttattgc
    47281 tcgagctggg taaagaataa accgactgta aaagtagaat ttgaagaaag gtggttgtga
    47341 tgctattaac aattcatgat gcaaatctaa agaaagtagc atttattgat aacgataaac
    47401 aagaaacttt aaactactat gatgacactt ggacacgcag cctagaaacg gggtcatcaa
    47461 cttttgaatt tacagtatac aagaaagcta taaagtctga tacagcgata aataaaacat
    47521 ataatttact gaatgagcgt gcttttgtct catttagaca caacggcaaa agctatgtat
    47581 ttaatgtgat gactgttgag gaaaatgagc aaactatcaa atgctattgc gaaaacctga
    47641 acttagagct tatcaatgag tatgccaatc cgtacaaagc tactaaagct atgtcatttg
    47701 ttgagtattg taatgcaatg gacttgttga atttcactca cctttctgtt ggcatcaatg
    47761 aaatttcaga ccaaaaacgg actctagaat gggatgggca ggataccaaa cttgcccgct
    47821 tactaagtct tgccaaaaag tttgatgcag agattgattt tgatacttac ctaaacgcag
    47881 atagctctat caaagccttt aaggtcaatg tttatcgtga aaacgatgca acccatcaag
    47941 gggtaggacg tgttagaaat gacatccagc ttacttatgg gaaaaatctt aaatcaatca
    48001 cacgtaagat tgataaaaca ggtgtctata atgctatccg tccaacagga aaacggacag
    48061 taaaaaatgg taaaggggaa gaagtagagg aaattgtaac catcggtagc cttggtgcat
    48121 ggtctgaaaa caacaaggat ggggtgcgtg aattttacca gcaaggagat atgctctacg
    48181 ctccattgtc aatgcagatg tatccatcaa cttttaccag ctctaccaca aatgaccaat
    48241 ggattaggaa agatatggag gttgatagtg atagtccatc agttattagg gcagcagcta
    48301 ttgcaaatct tagaaaaaac gcctatccag cattgaccta cgaagtggat ggttttattg
    48361 atgttgaaat tggtgatact atcaggattt atgatgacgg ctttactcct attttgcttg
    48421 tacaagcaag ggtatcccat caaaaaatca gttttaccaa tccagccagt aaccaaacaa
    48481 cctttgctaa tttcaaggct cttgaaaaca atctatctga tgggatacag gcggcttttg
    48541 agcgcttatt tgaggcatct aagccttacc ttattaagtt atccacagac aacggtgtca
    48601 tttttaagaa tcaaattggt cagagcctgg ttataccgtc cttgtatcga ggtggtaagc
    48661 cagttgtttc tggtgtcact tggcgttggt cactagacgg gaatatcaca acaggcatga
    48721 cttaccttgt ccaaggttca agcgtgacag atacggctac attgacggtt gcagcataca
    48781 ttggtaacga tgaggtggcg gttgatgagc tgacgtttgt aaacgtattg gatggtcgag
    48841 atggcggagt cgggcccaaa ggagaccctg gcaatgacgg tgtcgctggg cgtgatggga
    48901 ataaggttta tacctcaata tatgaaatga gtgccaacca atccggtatt tatttatccg
    48961 atttgacacc taccgcaaac cctgccaatc tccctccgat aggttcaata gttatagcac
    49021 cgtctggaaa aatgtatcct attattgcat tgaatccaac tagtactcct ccgtcgtaca
    49081 cagccggagc gctagtaggg aatattaaag ggccgcaagg cgataatggt caaacgccag
    49141 tagtgcattg ggcttattct gataatgcag acggtacagg attgaccaca tcagataatg
    49201 gtcagagata cattggacac tattcggact acacacaagc tgatagcaca gacaagacaa
    49261 agtatcgctg ggctgatagg tgggcgaaga ttgaagtagg cggacggaac tatctccgaa
    49321 acagcagttt tgtagatggt gtgtcctatt ggcaaaataa ttcgggagct gaacaatttt
    49381 cttggcggtc cgtcccagaa tttgcaacac agttagggcg aaaggggtgt atccatctct
    49441 acagtaaagg tcttccgctt gattccttag gtattttcca agtaatacaa gatatgcagt
    49501 tatcggaaca ggaaaaaatt accctgtcat ttgatgtagt ggggcgagag gggaacgata
    49561 gcggattgat ggtgattata aacaatcatt tgtttgacgt tagacagatt attcctgtta
    49621 gttccatacg cacctacagt caatttacta agactagcct tgagagaaaa gcattgacct
    49681 tcacaatccc caaagcttgt gatgccatta gtgtcattat catcttccat ggcggtataa
    49741 caggggatgt cgtcctaaca gatttcatgt tggagagggg gttagtggca acaagccacc
    49801 aggagtcgcc ttcggatatt gaagaccgca ttggtgtgaa agcggaccaa gcattgaccc
    49861 aggaacggct caacgcccta agcgagcagg caggtatcat gcaagcggag ctggaagcca
    49921 cagcgagtct ggatactgta aacaatatct tgaaacaaat caaagatatg aaagcagctg
    49981 acgaagctac atgggctaaa gttgaaaagg atctgataac tcacttacag cgtgtcataa
    50041 aaattgagac aaacctagga gaccaagcac agcgctggaa tgcggtagat acctttatgc
    50101 aggtctcaaa cgatggtttg tcactaggta aggcagacgg tagctccagc atgttgttta
    50161 gcccagatgg acgtatcacg atgttttcga gtggtactcc agtcatgtat gtggataaag
    50221 gggttatcca cattgacaac ggtatctttt caaagacggt tcaaatcgga cgttttagag
    50281 aagaacagta ccataacaat ccagacatca atgttaaacg gtatgtttat tagaaaggga
    50341 tagcttgaaa gtatggcagt atttagatat tcagggaact ggagaggttt cctagaaggc
    50401 acatcatcca ccgtcagtca agatataagc ggaaacagct cagtaatcaa gattgatgtt
    50461 tggataggaa tggacacagg gtggaacatt gagtttggta atacttacgg caataccgtc
    50521 accgtcactt gtgatggtca atatcaaact attgccgtag gtcctctata cctcaatagc
    50581 tccaaaaagc atttaggctc agtacagttt agagtggggc acaatgctga cggaacaaaa
    50641 tcagcaggaa ttggcttgag ttctaatatg agcaatatca gctatggaac tttgaacttt
    50701 ggtaatgctt cggggaactg ggttcatgga ttggcgacta tcccacggtc aagctctgta
    50761 agcgtcggca ctggtgttat tggtagtgca gtcactatca atatcaatcg tcagagctct
    50821 agttttaagc atattgtccg ctaccattgg ggcaacaaac aaggaacaat cgcaagcaat
    50881 gtagatacgt ctacaacttg gactatccca ctcgattttg cgaacgacat cccaaacgca
    50941 acaagtggta taggaacaat ctttgttgac acctattctg gtagtaccaa gacaggcacg
    51001 caacaggtcg cgtttacagc aagcgttcca gatagtatca agcccagttt gactggtttc
    51061 acgctgatag acggaaatac tgcagctagg acgctgattc caggagaaca acagtttgtg
    51121 cagattgttt cgaacatagc cgtgcacttt gggcaagcta caggtacata cggctcgaca
    51181 atcacaagtt atcacgcaga gatagttggc aagaaccagt ctactagtca aaacggtggt
    51241 agcttaggaa ttatgaacta tcatggtcag gttactatac gagcaagggt aacagacagc
    51301 cgtgggcgaa cgagtaacac gatagagcga actgtgacag tgttggaata ttttgcacca
    51361 gctttcaact ttagcgtgga acgttcagga gcgacatcaa gtacattctc tattctcaga
    51421 aacgctcgta tagctccgct gacggtaggt ggtagccaac ggaacacaat gactttaact
    51481 tttcgtgtag ctccagctga tagcaataat tacacgacag acaatggtcc ggcatctggt
    51541 acttttacga ccttggcgag cctgacaaat tcactggcca atctatcagg tacttattct
    51601 tctgataagt cgtgggatgt cataggaata cttgaagaca agttcactcg ttcggagttt
    51661 aaaatcaaag tttcgaccga agcggtagta ttcagctacg agaagggcaa ccgctttgcg
    51721 gttggtaaaa tcgtagatac gaaccttccg ggagggtctg tagagtcaac aggcgggtat
    51781 tacttgaatg gtaagccaat tcagaaccat caattgacag agaacactgg tcaagccata
    51841 cgggctttta gtgattggaa taattacgtt gataccggct tttatatggg agacaatata
    51901 ttaaatcagc cgccatttga tggtcaacac ccatggaaat atgtccgaac aacgaaacat
    51961 aattcgctat acgctttgca agaaagtgtg gatttttatg gtcgatctta ttctctacgc
    52021 gtaaaagaaa atggtaaatg gtacccttgg aaacaactgg ttatgtctga ccaccccaac
    52081 ctcatcaaca ctggttggca gtcggcaggg tttccaggta cttattacaa gcgtgttggt
    52141 gacatactga cgattaaata tgatttcagg ggtaacggat caacaatgaa catagggagc
    52201 ataccaagtg atatttgggt tgcgccacag tcctatatgt taataatcgc taagtggtct
    52261 attagcggtt ctgataacag ccatgtccaa atcaaccaag ggacaggagg acttaacgtg
    52321 ttgtctacag gtaacggagt cgaatataaa ggtcagataa ctattatgat ttagaaagga
    52381 aatatcatga agttcaaatt tttaaccaag agtacggagt ggttgggatc ttctccacat
    52441 cgtactattg tagttgtagg aaacgaagag ggagcatcta taccctatgc ctttgataaa
    52501 gaagctatca atttgtcgga tagcgagcta tttgatatgg ctatggaaaa aatgtatcaa
    52561 gaaaatttcc caaacagagc agaagatgag aaattcaatg cgattggcaa gcgtcttgcc
    52621 aagattgatg atattaccga agaagctaca aagaatcttg aaaaggttaa agagcaggtc
    52681 acgatgtctg cgtcatcccg tgctgcattc ttgcagatcg ttatgacatt gtatgggaag
    52741 gggttgctta cggatgaaga tttattgcaa actggtctat ttgatgatga agttgtcgaa
    52801 gagaccttgg aagttattta aaaataaaga ttggagaatg gatatgatga ttaaacttta
    52861 cgcaattgaa atttttgaag gaaatatcaa atataaagat ttgccttttt cagacattat
    52921 caaaaataaa attaaggctt acctcacaaa gatggttgaa gataaggaaa tcttggctga
    52981 attgattagc gaggagtagc ctatgcaaat taaaccagag catgtatatg ccttagtcgg
    53041 atttgtgtcc acagtcgtcg gattgtggac caatttctca gccaaaatca caaaacaaga
    53101 gaatcgtatt acgatattag aaaaggatat tgaaaacctc aaagagttca aagaaagtgc
    53161 taatcgacga ctagatagtc acgatgaaca aaacaaggcg attttggtcc ttgcggagca
    53221 ggtcaaaagc atgggagaag atattcgaga tctaaaacgc gtcattatga aagaggggta
    53281 acatttatga aaattaactg gggagtacgt ttacgcaata aaacattttg gtggacacta
    53341 gtaccattat tggtactttt gtctcaacaa ctgggcttta attgggtacc cgagaattgg
    53401 gaatcaacct ttgcgacgat tatgtctatc ttgactgttg tcggtatcat caatgacccg
    53461 acgactgcgg gagtatcaga tagcaagcag gctcttgact attacgagcc aaaggcagac
    53521 aaacgatgag aatattaaag acaacatttt gtgtgttggc gctgattatt ttggcgccaa
    53581 ttgcatttct acttgtacca attttggagg tattagatga caatcaatct tgaaacatcc
    53641 attcgttgga tgagcgaccg tgtcggcaaa gtctcttact caatggacta tcgtaacggt
    53701 ccgaatagtt atgactgctc tagtgctgta tattatgcgc taatggcggg tggtgcaatt
    53761 tctgcaggtt gggcggtcga cac
//
LOCUS       DATUCG010000003        44997 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.003, whole genome shotgun sequence.
ACCESSION   DATUCG010000003 DATUCG010000000
VERSION     DATUCG010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 44997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44997
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.003"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            58..1875
                     /locus_tag="VCA20_000122"
     CDS             58..1875
                     /locus_tag="VCA20_000122"
                     /inference="COORDINATES: protein motif:HMM:NF013080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEP1833997.1"
                     /translation="MLQLIQEKNGKWVGVYTVFLSSILLSSFYNYFLQSGVRIQNITN
                     SNTVEFMFNGALEFQTFILFVLFCLVYLIINKYLLATLVNIIVWSLVIYANISKFKQR
                     DEPLLLTDFTWLRKFSFLLKFINLNIFLFGVLLILGIAVGYTLYLKKVDTYKIINSIK
                     YRLVAIFTLIFLPYSLLSVSINNVSENYYFAELRNRTDFNWLGFSANSRFKSVTYLWA
                     QQFFSPIMKKPLNYSESNVKELVNKYTQISEQYNSERTNQIFDQTVIYILSESFINPE
                     RLSSLTVSRPIVPNIMEISNANTGGTMKSDGYGGGTANMEFQSLTGLPFYIFSDSISV
                     ANTEVFPKLKYIPVLSDSYNSENRIAIHPDSATNYNRNNIYDTLEFSKFIAKRDSKDV
                     FIKDEIVGIYPSDRSVYQTIVEELSETDNQFFSVITMQNHAPYSANDPLDIVVTGSNF
                     TEEENNSVTNYARLLNHTDNFTKDFLNSLTEINKKITVVFYGDHLPGIFPENTFTTNP
                     ESQFETDFFIWSNFETVKKDYSTIHSSDMTAVLLDHTNSKVSPYYALLTKVAQVDINY
                     KKQGMVKESEFLRDLELLQYDITEGEGYILNYPDFFEFK"
     gene            1887..3395
                     /locus_tag="VCA20_000123"
     CDS             1887..3395
                     /locus_tag="VCA20_000123"
                     /inference="COORDINATES: protein motif:HMM:NF021436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HEP1833998.1"
                     /translation="MKSKFFSHKLKLFVLSILIIMCSQLLLRMPINTNFNPLLVILIA
                     ISGLYFFLVDLSDSESLAKNALITILLFGTVISLAKPVQFGLDEETHLRNTLRLSDGF
                     IIQFEKENIPDYSSVYNHDILRSPDTYTDNTYWLNVEHQDSQFSGKIIGLNNIVYLPS
                     ALGWNLGKLISTKIFISYYLGRIANIIVFGLLIYLAIRWSRVYKEIVYLFGTFPAILY
                     ICAGFHYDYLYYAASLMLAALLTNILNQDSPLIDKQVIAYLSLTFLFTFAKFPYILLG
                     ALICLLPNRYYQSMKQRLRTCFYFSLQFILSFVYYINGSIIRRITGGVVESIPSETSL
                     GYFLTHPSPAIRTFFISTLSGSLNSFARALEYTIIESEVLQSISLILFLFLIFLLSLK
                     LKIKVSNMTKILIFTFFVGITLLIIYAISGDVRVYRPGDIWIGGVQGRYYYLMLLFLP
                     IILSPFLREMLMKDDYVIDPDNNVAKIMQHSIALLNILTIAIAIFTQTPHKL"
     gene            3461..4432
                     /locus_tag="VCA20_000124"
     CDS             3461..4432
                     /locus_tag="VCA20_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEP1833999.1"
                     /translation="MKSPKVSIIIPVYNAANTVKKCVSSILGQTFKDFELILINDGSS
                     DSSLSKIQKFMFDSRVRVIDKQNEGASQTRNRGIREAKGDYLLFIDSDDYIDKDYVEC
                     LYKEIEQSKFDMIISGIRMVDSSGKELNHFRLNDSPWSKYMITSPCTRIIRRKFVLEN
                     NLFFIDYTMEDIHFNAVFFSRTDRVKVIPYIGYNYLMNPESTTHTLHKGISKEVDILN
                     IISSIHTAVNPTDLVKYFYRKVYIYYLLKSGRYSTPEVFLNEYERIVDYIKTNQLEST
                     IRLFDKRISEEKFTTKFIISTFGVFEKFDLMSLFAKCYCIKNSREGF"
     gene            4439..5197
                     /locus_tag="VCA20_000125"
     CDS             4439..5197
                     /locus_tag="VCA20_000125"
                     /inference="COORDINATES: protein motif:HMM:NF025751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4422 domain-containing protein"
                     /protein_id="HEP1834000.1"
                     /translation="MSIFVITHKKFTPFTEELNYKIMLVGANNNKGEAFYLKDNFEED
                     NISDKNFSFCELTGAYSLYKHCTDDIIGLVHYRRYFMNSEGFPLSFNEAESILEKYDV
                     ILPTKSKLTSYKIPVSVKKHFISLLGKDGKEAWEKCSDILKRKYPEMIVSFENFERQN
                     STYFYNMCVMNSRDFKAYHEWLFDILLQLDKEIDVTSYSKYNQRMFGFISERLLTFWV
                     MYNNKKVKELPIMFTEPISFLNQVKQYVERKLDK"
     gene            5201..6661
                     /locus_tag="VCA20_000126"
     CDS             5201..6661
                     /locus_tag="VCA20_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004231348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HEP1834001.1"
                     /translation="MTSRTKSIKRNFILNFIFTVSGIVFPLLAFPYASRILLPEGTGK
                     VAFVSSIVSYFTMIGMLGVPVYGIRACAKVRNDKKLLSKTVFEIFILNSITMLLSLLL
                     YFITIVSLPRLSELKTLFYINSLTLILNLFGFDWLYKALEEYEFITKRTVLIKCFALI
                     LLFFLVKESTDYTEYSIILIMASTGSYVFNFLNLRKVIDFSNIGNLKFFHHLKPMFTF
                     FIMAVATTIYSSLDAIMLGFISGDQSVGYYNAAINIKNVLLSMITSLGAVLLPRLSVY
                     VQQQKFDEFRTLTLQALQFVVYTSIPLTFYFIIFAESSIYFLSGVAYYGSVLPMQVVM
                     PTLFLVGISNLIGIQILVPLDKETVVVKSVCIGAVVNLIINVLLIPIFGAVGAAVGTL
                     VAEIFVTAYQLYYLRTFLKDIIIQIRWKNILISTVLASISVYIFREVNNFDSPLIVLI
                     LSFIIFGMIYGIVLIMMKDDALILLVKGLKSKNEKL"
     gene            6648..7757
                     /gene="glf"
                     /locus_tag="VCA20_000127"
     CDS             6648..7757
                     /gene="glf"
                     /locus_tag="VCA20_000127"
                     /EC_number="5.4.99.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019789942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HEP1834002.1"
                     /translation="MKNYDYLVVGAGLFGAVFAHEAAKKGKKVKVIEKRDHIAGNIYT
                     KEVEGIQVHEYGAHIFHTSEKEIWDYVNQFAEFNRYTNTPVANYRGEIYNLPFNMNTF
                     NKLWGVVTPAEAEAKIAEQRAVLGGKTPENLEEQAISLVGTDIYEKLIKSYTEKQWEK
                     PCTELPAFIIRRLPVRLTYDNNYFNDTYQGIPIGGYTQIVEKMLDHENINVETNVDFF
                     ANKEEYLANYPKVVFTGMIDEFFDYELGELEYRSLRFETEVLDMENYQGNAVVNYTDS
                     ETPFTRIIEHKHFEFGAQDKTIITREYSKTWKRGDEPYYPVNNDHNNKLYTAYKRLAE
                     QQENVIFGGRLGHYRYYDMHQVIGAALQCVRNEMK"
     gene            7801..8517
                     /locus_tag="VCA20_000128"
     CDS             7801..8517
                     /locus_tag="VCA20_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEP1834003.1"
                     /translation="MKILMIIPAYNEEESILQTVQGIIDYRNSVNFQLDYVVINDGST
                     DHTKEILIQNKLNAVHLVQNLGIGGAVQTGYKYALDNDYDVAVQFDGDGQHDIRSLNS
                     LIQPILVGQADMVIGSRFVGDTLSEFQTSFMRRFGIGVISNMIKLTTGNRIWDTTSGY
                     RLGNRKVIAQFAKRYPIKYPEPESTVHLLKQNFQVVEAPANMFERAGGVSSITPFKSI
                     RYMVEVCSSILIASLMKEGE"
     gene            8517..8873
                     /locus_tag="VCA20_000129"
     CDS             8517..8873
                     /locus_tag="VCA20_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HEP1834004.1"
                     /translation="MTIQALAMFLASLCFLYFIFRNINKNKILFEHAFMWIVIGFGLI
                     VFALFDVIPIKLAYLFGFGLTSNFLLSVAIFVLLVIGFLHSMALSQQKQQIKNLIQEV
                     SMAKKRIAEMEESDAE"
     gene            8863..9651
                     /locus_tag="VCA20_000130"
     CDS             8863..9651
                     /locus_tag="VCA20_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002886818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEP1834005.1"
                     /translation="MQNNHTFVICAYGDSRYLEECIQSLVSQSLKTNIILYTSTPSDY
                     INGLCEEYQIPYYSSPGGGIGKDWNNALSFVQTQFATIVHQDDVYLTKYAEKILSYMT
                     DETLIAYSDYQEVKEGEVIALTSNLKIKQLMLRTLDIFPKWKFWRNRVLAFGNPISCP
                     AVTYNLNNLKDFKFNEEMKVSLDWFAWYQISQKKGRFTFVDESLMYHRIHEESETTNS
                     IENNIRTKEDYEMYLLFWPTFIAKFLLRYYVKSQDANGGNNGEK"
     gene            9620..10834
                     /locus_tag="VCA20_000131"
     CDS             9620..10834
                     /locus_tag="VCA20_000131"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEP1834006.1"
                     /translation="MQMGETMGRSKTVTNTRTRKRTYWWFNLYVVVLIGLLVGYKHFS
                     LLRDGYIPLNTVLEKQSFKKISDWQYEKKIGDLSYSLLIDTDKNSVTKAGYQFDIRNN
                     IQHFLWMDYLSADKIEEIFNVQVSSSGIFVDVKDIEFSQHQWTEEFPNLIAHAGGTYR
                     EKAYNTFYMNSLEALQQNYTLGHRVFELDFYLTSDGKMAAVHDWEQFGHMNGLALSSE
                     EWKNFQTFGSPVTESRFTTMLIGDVLDQMLINKDMFLVTDTKSFEVSEEEVIHQFTEI
                     YNEAMKRSPELLSRIIPQIYNQTMYTTLKKVYDFSNVIYTLYVSPDSPEQVIEFVANN
                     PSIKVVTIPLNHGGYFNSEFFNNLHALDKKIYTHTIHTYDELTKYSALGIDGFYTGLL
                     LPSDMERLSSLR"
     gene            11015..11503
                     /locus_tag="VCA20_000132"
     CDS             11015..11503
                     /locus_tag="VCA20_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HEP1834007.1"
                     /translation="MIKIFGKIRYHWQPDLSWVIIYWSLTFTPIFIGMTLLLEKLRVS
                     RLFILLVSLFAVLVVLGLHRYFEIKDEHLRIASANPFAVLKIEIATIEKIEVSYLAIR
                     IFSQEFPNGQVYHMRKWPKKYFINHLAIHNCFKGEIELIDHLIKQDYFEEYYTKKAKS
                     IR"
     gene            complement(11492..11686)
                     /locus_tag="VCA20_000133"
     CDS             complement(11492..11686)
                     /locus_tag="VCA20_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEP1834008.1"
                     /translation="MKVKLIPERCIACGLCQTYSSLFDYDGDGIVVFSDESQFEKTIE
                     PNPDILKAIKSCPTKAISAD"
     gene            11737..12213
                     /locus_tag="VCA20_000134"
     CDS             11737..12213
                     /locus_tag="VCA20_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEP1834009.1"
                     /translation="MSQEPWNEEVYHTETTSRKERLSKGVANTTVFTVLAIIFFVIVL
                     IIGIMAVYLSIGGGSKTDSTKEFYTPSTTTVVAESSSSAAETQESTSTDTTETETSSS
                     PAEATDGSTLTVQAGEGVGQLAARGGISIAELERLNPEKMTTGSWIAHPGDVVRIR"
     gene            12223..12900
                     /locus_tag="VCA20_000135"
     CDS             12223..12900
                     /locus_tag="VCA20_000135"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEP1834010.1"
                     /translation="MKSIQIAIDGPASSGKSTVAKIIAKNLGYTYLDTGAMYRSATYL
                     ALTNGIEVTDQKRIVALLAQYPIRFGRDENGQQLVFVGDEDVTLPIRDNQVTNNVSAV
                     AALPLVREELVRLQQDIAQAGGIVMDGRDIGTVVLPQAELKIFLVASVEERALRRFKE
                     NTERGIETDLESLKEEIAARDYKDSNREVSPLKAADDAITFDTTGVSIEGVVQFISEK
                     AKEILDR"
     misc_feature    12927..13055
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            13081..13602
                     /gene="infC"
                     /locus_tag="VCA20_000136"
     CDS             13081..13602
                     /gene="infC"
                     /locus_tag="VCA20_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEP1834011.1"
                     /translation="MAKQDLFINDEIRVREVRLIGLEGEQLGIKPLNEAQAIADSANV
                     DLVLIQPQAKPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPVIDKGDFET
                     KLRHARKFLEKGNKVKVSIRFKGRMITHKEVGAKVLAEFAEATQDIAIIEQRAKMDGR
                     QMFMQLAPASDKK"
     gene            13629..13829
                     /gene="rpmI"
                     /locus_tag="VCA20_000137"
     CDS             13629..13829
                     /gene="rpmI"
                     /locus_tag="VCA20_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEP1834012.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAYTSHRFHGKTKKQRRHL
                     RKAGMVHSGDFKRIKSMLTQMR"
     gene            13879..14238
                     /gene="rplT"
                     /locus_tag="VCA20_000138"
     CDS             13879..14238
                     /gene="rplT"
                     /locus_tag="VCA20_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEP1834013.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHLLFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AAAFTALADAAKAKLAK"
     gene            14675..17179
                     /locus_tag="VCA20_000139"
     CDS             14675..17179
                     /locus_tag="VCA20_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEP1834014.1"
                     /translation="MKQGKTFLYVAGVSLICAVALVTYQASNYREQSNHQKTTSIETS
                     QSKQSKEIPSDSKKEVPGIDKATDDGFLLTDESQIEEKTDLGIIVNHGDHKHFFFYSD
                     LKNTKWAYLIPENYQEKNHSSQPKRSQLSSGSKQIEDEYVFDPKDIVAEDANGYTVRH
                     GDHYHYILKSSLGGTTHRQLTSDSRQSPTVPIVHQQEGIPGIDFQTSDGFLFDGQNIS
                     GVTETGILVRHGKHLHLIHFETLKRSKWSYLVNQYKPNVESDKKSQSEVENTEYQMKL
                     DYLAKELNLEPSRLKKVIVDGQIGLEYPHGDHTHIVLLKEIDTTKPFESPEDRILKQK
                     DGETLEQRKERLIKQYMERFKVKREDITIDGNYMSVRHGDHAHVYKIDPSLPDDPERD
                     VKTETVNLAIEKQLVYGPFYTEGSTENLTRNGVHQKYHPEGLQDIKNFILVTFSTNSD
                     FGDFVVNGKKTKRVYYLVRKDLNWKDLNIAYPQTLQQKGRVFNGWNATLPKSGKMARE
                     HQSFYADFDNIYRKPTKNIYTPSDDVSNIDLSDYVPVKYSAIANGRLKLNGEIRAGFI
                     YFVKSDLTWKQAKEQGLVVPEPVPSKDYEFIEFRTVITGGEKDTDYVSATASLAAFGT
                     TAPRIGPYIANNTENPTDINDPSRHPNYYWHDPKNYVALAFRAGEGGQLQTHLGTSKT
                     VVYLVRKGLTLNQAGIFPPSLKSDTGYKRDYTKPVIPNVAWDTPILEDTVYDIHFDKV
                     ESDSKDGTVEPGDTWLPGNSLPNTPEVPNGEENADSWLDDLLTPSPEATDVETVTEAE
                     TTTEDTKATESSTAAPIDVPKKSSTEEELSEDSIFP"
     gene            complement(17476..19656)
                     /locus_tag="VCA20_000140"
     CDS             complement(17476..19656)
                     /locus_tag="VCA20_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEP1834015.1"
                     /translation="MKKLIDYVKKSGQTRLGFVLILLIIYWLKTLWAYYVDFNLDTQG
                     FFQNFIAIMNPLPVGILLLGLSLYIKNTKVFYSVAMIIYIILSVWLFSNAVYYREFSD
                     YISISTIMATSSVSAGLGEAALKLFRLWDIVYFLDFIIFGILVYRKLLRWDKKTFHKK
                     SSFAITALSVMLFSINLFLAEIDRPELLTRGFSNTYIVRALGLPAFLGYNANQTYNAH
                     KNRSGASATDLEPVKQYITEHYAQPNDNYFGIAKGRNVIYLHLESLQQFVIDYKLNVN
                     GVEYEVTPFLNSLYHSSSTLAFSNFFNQVKAGKTSDAETMIETSLFGLNQGSFMVQYG
                     GSNTQQAAPYILSQNGGYTSAVFHGNAASFWNRNNTYKQWGYNYFFDQSYFSKATAEN
                     SFQYGLNDKIMFADSIQYLEHLQQPFYTKFITVSNHYPYTTSLIGDEIGFPLANTDDE
                     TINGYFATANYLDASIKAFFDYLKATGLYENSVIVLYGDHYGISNSRNPDLAPLIGKD
                     SQTWSDFDNAMMQRVPYMIVVPGMTNGGIFDTYGGEIDALPTLEHLLGIKSDNYLQVG
                     QDLLSSQHSQIVALRTAGSFITPKYTSYEGKIYYTETGLEITNPDETTQKEIDAIKEA
                     TAAQLATSDAIQTGDLIRFFDNGLPALDSSQYNYIDSLAAEIALEKKLGTQSTSLYSK
                     NGNKSTIELFKAPSYLELHGSTTATSTPNTGTSTSSTSTSSNSR"
     gene            19772..20935
                     /locus_tag="VCA20_000141"
     CDS             19772..20935
                     /locus_tag="VCA20_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEP1834016.1"
                     /translation="MKIKVNRIAEQKINRGIQLLDSADFSGFSFDEDRLAVLYSESGS
                     YLGQAYLSKQNKGIGWLFSRSNQDLTKDFFVKLFVDAKQKRQHFYQSEMTNAFRLFNQ
                     DGDSFGGMTVDLYDEYAVFSWYNTFIYSVKEIIVAAFQQVFPEVRGAYEKIRFKGLAF
                     ESAHLYGEEAPEYFTVLENGVHYQVFMNDGLMTGIFLDQHEVRGSLVDGLAAGKSLLN
                     MFSYTAAFSIAAAMGGAGETTSVDLAKRSRELSKAHFKVNSLELDNHHFVVMDVFEYF
                     KYAKRKGLTYDVIVLDPPSFARNKKQTFSVAKDYHKLISRSLEILNTGGTIIASTNAA
                     NVSVEKFKRQIEKGFGVRKHRYEASYRLPADFVINKNDESSNYLKVFTIQVDK"
     gene            20932..21606
                     /locus_tag="VCA20_000142"
     CDS             20932..21606
                     /locus_tag="VCA20_000142"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEP1834017.1"
                     /translation="MKIVVPIMPRNLEEVEAIDVERLAEADIVEWRADSLPKDDILRV
                     APAIFEKCNGKEVVFTIRTTREGGHLDLDDQEYVNLIKEVAALYQPDYIDFEYYSYKS
                     VFEQMLEFPNLVLSYHNFEETPSNYMEIMSELTSLSPAVVKMAVMAKTEQDVLDVMNY
                     TRGFKSLNTEQTFATIAMGELGKLTRIAGVITGSCWTFASLDETSAPGQMSLSNTRKF
                     LEILEN"
     gene            21610..22776
                     /gene="aroC"
                     /locus_tag="VCA20_000143"
     CDS             21610..22776
                     /gene="aroC"
                     /locus_tag="VCA20_000143"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002997861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEP1834018.1"
                     /translation="MRYLTAGESHGPRLTAIIEGVPAGLPLTAEDINEDLKRRQGGYG
                     RGSRMQIETDRVEITAGVRHGKTTGAPITLNVTNKDHQKWLDIMAVEDIEEKLKSKRR
                     IKHPRPGHADLVGGMKYRFDDLRNSLERSSARETTMRVAVGAVAKRILAELDIEIANH
                     VVVFGGKEIDVPDGLSVAEIKERAAQSEVSIVNQEREEEIKAYIDQIKRDGDTIGGVI
                     ETVVGGVPAGLGSYVQWDKKLDAKLAQAVVSINAFKGVEFGVGFQAGYLKGSQVMDEI
                     LWAQEKGYTRRTNNLGGFEGGMTNGEALIIRGVMKPIPTLYKPLMSVDIDTHEPYKAT
                     VERSDPTALPAAGVVMESVVATTLATEILEKFSSDNMEELKAAVASHRDYVKNF"
     gene            22784..23887
                     /locus_tag="VCA20_000144"
     CDS             22784..23887
                     /locus_tag="VCA20_000144"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEP1834019.1"
                     /translation="MKKTILIVGLGLIGSSLALCIRKDHPDYHLIGFDPNEQSCQIAL
                     GKGMVDEVSTELEGVAKQADVILLCVPIQVSLRLIEEFSALELKDMVLITDAGSTKSA
                     IVAAAEEHLAPRQINFIGGHPMAGSHKSGASAADLHLFENAYYIMTPCQATKQDAVPR
                     LTDLLSGTGARFVQIDAAEHDRVTSQISHFPHVLASSLMHQAGEYAQDHPFTNNFAAG
                     GFRDMTRIAESEPSMWTSILLTNADSILNRIEEFQARLSEISDVLRSGDQKAIWDFFD
                     KGRQTRRAMEIHKRAGVDSFYDLFLSVPDEEDTILKVLEVLRGISIVNIRINEENRED
                     IHGILQITFKNKGNLEDAAKRLREQTTYKIHLD"
     gene            23897..24235
                     /locus_tag="VCA20_000145"
     CDS             23897..24235
                     /locus_tag="VCA20_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HEP1834020.1"
                     /translation="MSTNIYDIANELERAIRNLPEYKAVEAVKASVEGNSEAKEILES
                     YISFQKEIQSKLQAGEIPTEADQKKMLDFNKKVQGNPLLTEYFSKQQQLGTYVADLER
                     IIFKPLNELL"
     gene            24497..25642
                     /gene="nagA"
                     /locus_tag="VCA20_000146"
     CDS             24497..25642
                     /gene="nagA"
                     /locus_tag="VCA20_000146"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEP1834021.1"
                     /translation="MTVFIKAKSIILPESEVESAYLEITDKGQFGRIVTEKPEGEIVD
                     YSDFHLAPGLVDTHIHGYASHDVMDNDFEGIKVISEGLLSCGVTSWLPTTLTDTTENL
                     DAVCETIGTYAGQETGAKIQGIFLEGPFFTEKYKGAQNPKYMSDPSIEKLDNWHSLSK
                     GLVNKIAIAPEREGVKEFIEFANSKAIHTALAHSDATYTQAEAAVEAGANIFVHVYNG
                     MSGLHHREPGMVGAALNLKNVYAEMICDGHHVHPAAAEIVVKARGVEETVLITDCMRA
                     GGMGEGESRLGEFEVVVKDGTARLKESGSLAGSILELIEAVQNVVKWGLVSLPDALRM
                     ASLAPARSVNIDHICGRIAEGRAADFIVVDDAGRLQATYLDGVKRFG"
     gene            25666..26667
                     /gene="lacD"
                     /locus_tag="VCA20_000147"
     CDS             25666..26667
                     /gene="lacD"
                     /locus_tag="VCA20_000147"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEP1834022.1"
                     /translation="MTKLQLSQAKFNHMTRLSNSDQVIAALAIDQRGALKRLLAAAAG
                     GGEFGDDILIDFKKVISSDLTPYASSILLDAEYGVPASELRHADCGFIAAYEKTGYDA
                     STPGRLPDLLPNWSAKRIKELGADAVKILLYYDVDDKPEINNIKHAWVERIGSECIAE
                     DIPYFVEILTYDASDMDVTSREYAALKPHKVNGAVREFSKSRYNADVLKVEVPVNMNF
                     VEGYTDEEPVYTVEEAKAFFKEQTDSTHLPFIYLSAGVSAKLFQETLVFAKEAGSSFN
                     GVLCGRATWKDAVAIFASKGEEAAKAWLADQGRRNVEELNEVLATTARPWTEKVEVK"
     gene            27361..28320
                     /locus_tag="VCA20_000148"
     CDS             27361..28320
                     /locus_tag="VCA20_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEP1834023.1"
                     /translation="MGFKKIILSSVVLLSAVTLAACSSNSSDSSSVNVGIIQYAEHEA
                     LSSAREGFVEALEEAGYKEGENLTIDLQNAQGDQANLQTMVEKLAGKNDLNFAIATPA
                     AQAMLNADSETSSVFTAVTDPVEAGLVESLEKPEGIMTGSTDATDVEGQIEMLLKVVP
                     TAKTVGIFYNSSEVNSEVQAEQAKKALEAKGVKVEVTTVTTTNDVQQAITSLAGKVDA
                     IYLPTDNTVASTASTIGDILKEAKVPAMGSDAAVIDAALFTYGVDYHAIGVQAGELAV
                     KILKGEKPADLAVEKPNTAAITVNEEMAKAVGIDAATIKALEE"
     gene            28344..29318
                     /locus_tag="VCA20_000149"
     CDS             28344..29318
                     /locus_tag="VCA20_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEP1834024.1"
                     /translation="MYYKLVKKLATISVASMGLLTLAACSSSSEQASSDVVKVGVLQY
                     MEHESLTAARKGFVVELEANGYKEGEELVLDYQNAQGDQANLQTISEQLIDGNDIVLA
                     IATPSAQSLATVSTETPIAFTAVTDPLSADLVESIEKPGGLLTGTSDQAPIDKQVELL
                     GQAVPDAKTVGILYTTSERNSEVQVEQAKELLEKAGYKVVVKGITSSNEVQDATTSLM
                     KDVDALFIPTDNTVASTMTMIGELSVEHRVPVIGGSTDMVDEGGLLTYGTNYEALGRQ
                     TAKMAIKIIEGANVSETAVEYPETVSLHVNEEMAQKLGIDTSKLAVSE"
     gene            29373..30266
                     /locus_tag="VCA20_000150"
     CDS             29373..30266
                     /locus_tag="VCA20_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP1834025.1"
                     /translation="MDIILSSISQGLLWSVMAIGVYLTFRILDIADMTAEGSYPLGAA
                     VCATGIVNGVNPLLATFMAMVAGMGAGLISGLLHTKLKIPALLTGIVTLTGLYSINLK
                     ILGKANVALLKQETLVTQLQDFGLTKTNAVLVIGLVFVLAVVGLLTLLLNTQIGLAIR
                     STGDNIPMSEANGINVDNMKIYGYMLSNGLIALCGALLTQNNGYADLNSGTGTIVIGL
                     ASVIIAEVILRNLRLGWRLLSVVLGAVVYRLIILAILEIPGMDADLVKLFSAILLATV
                     LYVPELQKKLNIRRPKLNGNA"
     gene            30276..31076
                     /locus_tag="VCA20_000151"
     CDS             30276..31076
                     /locus_tag="VCA20_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1834026.1"
                     /translation="MSTILSIQNIHKTFEAGTVNENHVLRGLNLDVKEGDFISIIGGN
                     GAGKSTFMNSLAGALTVDSGDILLEGKSIKHVSAAKRSKDISRVFQDPKMGTASRLTI
                     EENMAIAYRRGLSRGLGWGVKDSERAIFKEALKELGLGLENRMKVDTQFLSGGQRQAL
                     TLMMASLVKPKVLLLDEHTAALDPKTSDMVMELTKKIVESHQLTALMITHNMENAIEY
                     GNRLVMLHRGQIVVDVEGEEKKNLTVQNLMDLFYKNSGEKLTDDEMIL"
     gene            complement(31280..32347)
                     /locus_tag="VCA20_000152"
     CDS             complement(31280..32347)
                     /locus_tag="VCA20_000152"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEP1834027.1"
                     /translation="MKLTVNLPNTPYDILIQRGSLAQTGAWVKELWKPQKIAIITDDH
                     VGSLYRETVQSSLEQAGFETIVFEFPEGEASKNLDTVNQAYEFLVKNGMTRSDGIIAL
                     GGGVVGDLAGFVASTYMRGIHFLQIPTSLTAQVDSSIGGKTGVNTPFAKNMVGTFCQP
                     DGILIDPDTLKTLGKRELIEGMGEVVKYGLIDDVELWETLDQLDGSVESILEHADYII
                     YHSCEVKRKVVVEDELDNGVRLYLNFGHTIGHAIEATAGYGQVMHGEAVAIGMVQISR
                     AAEKKGLMPAGMTEKIIAMCEKFGLPTTHQPWNVDELYAALTHDKKARGKTIKLVIVP
                     QLGQAAIHQIPMEEMLEFLQV"
     gene            complement(32360..33229)
                     /locus_tag="VCA20_000153"
     CDS             complement(32360..33229)
                     /locus_tag="VCA20_000153"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEP1834028.1"
                     /translation="MNIDGYTRLAAVVAKPIKHTISPFIHNLAFKETGVNGVYVAWEI
                     PEEDLAVTLENIKRYDMFGINLSMPYKQAVIPYLNSLTDSARLIGAVNTVIHQDGLLI
                     GHNTDGIGFFKSLEKLRGFQVRNKRLTILGGGGASTAIIAQATLDGAKEITIFCRQQS
                     LERTQTSLTPIAQATGVPIQVLALEDSQLLQEHITNSDLLVNGTSVGMDGYSQPVPST
                     IRFPENLLVADVIYQPFETPLLKLAQSQGNPTINGLGMLLFQAAEAFQAWTGQEMPTD
                     LIWDQLVQKYDIK"
     gene            complement(33274..34299)
                     /locus_tag="VCA20_000154"
     CDS             complement(33274..34299)
                     /locus_tag="VCA20_000154"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019785454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEP1834029.1"
                     /translation="MGIHKRSTSLDIDKVKELSKLEGEFLAAKNQRDEELKRIIRGED
                     DRLLLVIGPCSSDNEEAVLEYARRLSKLQEEVKDKIFMVMRVYTAKPRTNGEGYKGLV
                     HQPDTSKLPDLINGIAAVRNLHYRVITETGLTTADEMLYSANYPLVEDLVSYHAIGAR
                     SVEDQEHRFVASGVDMPTGMKNPTSGNLTVMFNGIYAAQNKQNFIYRDAEVDTDGNPL
                     AHAILRGANTEKGGYEPNYYYDILLKTIKQYEQFGLKNPFIVIDTNHDNSGKNYLEQI
                     RIVRQTLINRAWNESIRKYVRGFMIESYLEDGRQDSPEVFGKSITDPCLGWEKTEALI
                     CEIHQTV"
     gene            complement(34309..35346)
                     /locus_tag="VCA20_000155"
     CDS             complement(34309..35346)
                     /locus_tag="VCA20_000155"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEP1834030.1"
                     /translation="MKEGDLLFTPISEKIDINQVREHSKLSPETLAKKQARDRELEAI
                     LKGEDDRLLLVIGPCSSDNEEAVLDYAHRLAKLQEEIKDKIFMVMRVYTAKPRTNGDG
                     YKGLMHQPDTDAAPSLINGIKAVRNLHYRVITETGLTTADEMLYPENLPLVDDLVSYI
                     AIGARSVENQQHRFVASGIDVPTGLKNPTSGNLKVMFNGLFAAQKKQSFLFNNTEVET
                     SGNPLAHVILRGAVNENGKYLPNYYYDNLLETIELYDQFGLKNPFIIIDTNHDNSGKN
                     YLEQIRIVRQTLINRDWNESIRNYVRGFMIESYIEDGRQDNPDIYGKSITDPCLGWEK
                     TQELIREIYNR"
     gene            35558..36838
                     /gene="aroA"
                     /locus_tag="VCA20_000156"
     CDS             35558..36838
                     /gene="aroA"
                     /locus_tag="VCA20_000156"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEP1834031.1"
                     /translation="MKLRTNIEKLQGKIRVPGDKSISHRSIIFGSLAKGVTRVHDILR
                     GEDVLSTMQVFRDLGVKIEDNGDIVEVHGVGFDGLQAPKNDLDMGNSGTSIRLISGVL
                     AGQDFEATMFGDDSLSKRPMDRVTIPLSQMGVEISGQTERDLPPLTIKGNKNLKPIRY
                     QLPVASAQVKSALIFAALQAEGESVIVEKELTRNHTEDMIVQFGGQLEVNGKEIRIQG
                     GQEFIAQEITVPGDISSAAFWLVAGLIVPGSKIVLENVGINETRTGILDVIKAMGGKM
                     TLSNIDELAKSATITVDMSELKATEIAGELIPRLIDELPIITLLATQAHGTTIIRDAE
                     ELKVKETDRIQVVADALNSMGATIEPTEDGMIIHGPTALHGAEINTFGDHRIGMMTAI
                     AALLAKDGEVVLERAEAINTSYPAFFEHLNSLMD"
     gene            36847..37338
                     /locus_tag="VCA20_000157"
     CDS             36847..37338
                     /locus_tag="VCA20_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEP1834032.1"
                     /translation="MPIVLLGFMGVGKTTTAHLLNLPVYDMDHIIEERIGMPIAAYFS
                     LEGEASFRQLETEVLKELLDLPSNCIVSTGGGVIKSEVNRELLLANREDNVLLTASFE
                     VSYQRIRKDRQSQRPLFLQCSKEEFEALYRERMALYQGLADTVIDTDKLNPEQVARKI
                     LCK"
     gene            37329..38156
                     /gene="pheA"
                     /locus_tag="VCA20_000158"
     CDS             37329..38156
                     /gene="pheA"
                     /locus_tag="VCA20_000158"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEP1834033.1"
                     /translation="MQVAYLGPRGSFTHQVAQQAFPSADLQAFETITEVIKAYETGEV
                     TYSVIPVENSIEGSVHETIDYLFHQAEIHAVAEIVQPIAQQLLATSADKTVEVIYSHP
                     QAIAQGKKYIQEHFPQARIEITASTAYAARFVAEHPEQPFAAIAPQAAAVEYGLEVIA
                     QDIQEISENFTRFWILGDKAPSIALEQVAKKVSLALTLPDNLPGALYKAMSVFSWRGI
                     SLTKIESRPLKTALGEYFFILDLDNQSEELLAYSQKELTSLDITYKVLGNYLVYMTS"
     gene            38168..39490
                     /locus_tag="VCA20_000159"
     CDS             38168..39490
                     /locus_tag="VCA20_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEP1834034.1"
                     /translation="MNKNNNILTHHEQLRLDYLQKNIHYLNDKESRELDYLLYKKELR
                     ERHGSPRQSLRSFTPTAYEDDFEEDYFEEDDEQEELLPRYPERKSRKTRQAKPQRFQS
                     PRIEPKAKVPKPKKARKKGGFKRFIGFVLLAIALVIVAMGYSFFKGMNSVSEKPVAEV
                     FNGVDTSNGTNILILGTDGRVGESSGETRTDTIMVLNVNNTDNKVKLVSFMRDTLVDI
                     EGYEYKLNTAYTLGEQDNQQGAEEVRKALKNNFDIDIKYYAMVDFATFATMIDTLFPE
                     GVTINARFSTINGEVVSSVEVPNDLQLEENAPAYQTIQVGVQQMDGKTLLNYARYRSD
                     DEGDFGRTRRQQEVLSAVMTQAKNPMKLFSGFEAIGKVVAMTPTTVPQSFMWLQGPGL
                     LLDAANGIERVTIPENGDWIDDYDMYGGMALRVDFEKYQQRLAELGLR"
     gene            39557..40918
                     /gene="rlmD"
                     /locus_tag="VCA20_000160"
     CDS             39557..40918
                     /gene="rlmD"
                     /locus_tag="VCA20_000160"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEP1834035.1"
                     /translation="MLKKNDIVEVEVVDLTHEGQGVAKIDGFVFFVDNALPGEKISMR
                     ILKLKKNIGFGKVEQWQSFSPNRNQDLDLTYLRTGIADLGHLTYPAQLAFKKKQVENS
                     LRKIAGISEIAVADTLGMDNPLAYRNKAAVPVRRVNGQLETGFFRKNSHDLVPIEDFY
                     IQHKEIDALVLATRDLLRKLDLKPYDEKEQTGLVRNIVVRRGHYSGQLMLVLVTTRPK
                     IFRVETLIERLTSQFPNLVSIVQNTNDQNTNAIFGQEFRLLHGSETIADTMLGNEFEI
                     SAPSFYQVNTEMAEKLYQTAIDFAELSADDVVIDAYSGIGTIGLSFAKQVKHVYGVEV
                     VEKAVLDSQKNAARNGIDNVTYVCDSAESAMQKWVVDGIKPTVIFVDPPRKGLTESFI
                     KASTSVKPEKIVYISCNVATMARDIKLYEELEYKLTKVQPTDLFPNTHHVECVVLLQR
                     SNG"
     gene            41129..41296
                     /locus_tag="VCA20_000161"
     CDS             41129..41296
                     /locus_tag="VCA20_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834036.1"
                     /translation="MKKKEQSSRQIVMCHLVAILGVDIEKATHLVDELEQVGLIRFDE
                     FGNVGLLVLEG"
     gene            41299..42118
                     /locus_tag="VCA20_000162"
                     /pseudo
     CDS             41299..42118
                     /locus_tag="VCA20_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648301.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
     gene            42267..43616
                     /gene="dcm"
                     /locus_tag="VCA20_000163"
     CDS             42267..43616
                     /gene="dcm"
                     /locus_tag="VCA20_000163"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEP1834037.1"
                     /translation="MKFLDLFAGIGGFRLGMESQGHKCLGFCEIDKFARTSYKAMFNT
                     EGEIEYHDIKEVTDHDFRQFRGQVDIICGGFPCQAFSLAGRRLGFEDTRGTLFFEIAR
                     AAKQIQPRFLFLENVKGLLNHDEGRTFATILSTMDELGYDVEWQVLNSKDFQVPQNRE
                     RVFIIGHSRRYRSRFIFPLRRENSPAHLERPGNINPSKRGSNGEVYLTNGLAPTLTRG
                     KGEGAKIAIPVLTPDRLEKRQHGRRLKDNQDPMFTLTSQDKHGVVVAGNLPTSFDQTG
                     RVFDISGLSPTLTTMQGGDKVPKILLREELPFLKIKEATKTGYAKATLGDSVNLAYPD
                     STKRRGRVGKGISNTLTTSDNMGVVIAALEYRQDKWYEVTGIVLEGKLYRLRIRRLTP
                     RECFRLQGFPDWAYERAESVSSKSQLYKQAGNSVTVTVIEAIAREFRRTKNGRGRKT"
     gene            43613..44041
                     /locus_tag="VCA20_000164"
     CDS             43613..44041
                     /locus_tag="VCA20_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834038.1"
                     /translation="MNLLHKKSILECTELEEHIHQVETNQLLQKILSLPNFDCDFEVT
                     FEDDYHNEMNDPLFYESNLHQISDFMETRDIKNGVDTLLTKDNHLAFRAFGENYSARG
                     KDGILTTLVTVKCFGEGRMPIDMSRYFSTPEPTVENSLTL"
     gene            44052..44435
                     /locus_tag="VCA20_000165"
     CDS             44052..44435
                     /locus_tag="VCA20_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834039.1"
                     /translation="MLEVYLGNNANTNQDLLTILTTYGVTYRCINACEVNREILLSLF
                     AKTTDCFELLSPRFLRFKRQYTISLNEMIQLILQKPDQNLRLPLIVCQNHIYPAIGLD
                     EVRTFLPRQVKEELFQASLMKQVTG"
     gene            44444..44677
                     /locus_tag="VCA20_000166"
     CDS             44444..44677
                     /locus_tag="VCA20_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003065178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEP1834040.1"
                     /translation="MNERFWDNLEIILAEKDLTWAELARKVFNGQYVYPSEFNRLYQK
                     LRHYKSNRLMPQTRWVERIVLVLDIDYEDLFKR"
     gene            44680..>44997
                     /locus_tag="VCA20_000167"
     CDS             44680..>44997
                     /locus_tag="VCA20_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEP1834041.1"
                     /translation="MIRIVLFYLAIQLNGLLVSLYLKEYLKIEGIVLLQLVLLSVTCL
                     EIARHKTVQAKKITLSNRLRCLLLGFVCMVAFAVFISFLSPVQTRNQAVLVQVGKQVP
                     PIIF"
BASE COUNT        13956 a         7595 c         9659 g        13787 t
ORIGIN      
        1 tcggtattaa ctgaattgtt tattggtgcc ttgttcataa tatttctttc taatggtttg
       61 ttacaattaa ttcaagaaaa aaatggaaaa tgggtaggtg tttacactgt atttctaagt
      121 tccatattac tctcatcctt ttataactat tttctacaaa gtggagttcg catacagaat
      181 attaccaatt caaatactgt agaatttatg tttaacggtg ctttggaatt tcaaacgttt
      241 atattatttg tgttgttctg cttagtatat ttgattatta ataaatattt actggctaca
      301 cttgttaata taattgtatg gtcacttgta atttatgcaa atatttccaa gttcaaacaa
      361 cgtgatgaac ctttattact gaccgatttt acatggttaa ggaaatttag ttttctactg
      421 aagtttatta acttgaatat atttttattc ggggtgttac taatattagg tatagcagtt
      481 ggatatacat tatatttaaa aaaagttgat acatataaaa taataaatag tattaaatat
      541 agactagttg ctatctttac tctgattttc ctgccttact ctttgttatc tgtttcgata
      601 aataatgtat cagagaatta ttattttgct gaattgagaa atcgtaccga ttttaattgg
      661 cttggttttt cagctaactc cagatttaaa tcagtaacgt atctttgggc tcaacaattc
      721 tttagtccga taatgaaaaa accacttaac tatagtgagt caaacgttaa agagttagtc
      781 aataaatata ctcaaatttc agaacagtat aatagtgaaa gaacaaatca aatttttgat
      841 caaacagtaa tttatatttt gagtgagagc tttattaatc cagagagatt gtcttccctt
      901 acggtttcaa gaccaatagt tcctaatatt atggaaattt caaatgccaa tactggagga
      961 actatgaaat cagacggtta cggtggagga actgccaaca tggaatttca atcattgaca
     1021 ggtttaccgt tttatatttt ttctgactcg atttcagtag caaatacaga agtttttcca
     1081 aaattgaagt atataccagt acttagtgat tcatataatt ctgaaaatag gattgcaatt
     1141 catcctgata gtgctactaa ctataataga aataacatat atgatacctt agaattttct
     1201 aaatttatcg ctaaaagaga tagtaaggat gtttttatta aggatgaaat tgttggaatt
     1261 tatccgagtg atcgctcggt ttaccaaacg attgttgaag aactctctga aacagataat
     1321 caattctttt cagtgattac aatgcaaaac catgctccat attcagctaa tgaccccttg
     1381 gatattgttg ttacaggtag caactttact gaggaagaaa ataatagtgt tacaaactat
     1441 gctagattac taaatcatac agataatttc acaaaagatt ttttgaatag tttgactgaa
     1501 attaacaaga aaataacagt tgtattttat ggtgatcact tgccgggaat ttttcctgag
     1561 aatacattta ccacaaatcc agagagtcag tttgaaacag atttttttat ttggagtaat
     1621 tttgaaacgg ttaaaaagga ctattcgacg attcattcaa gcgatatgac tgcggtgtta
     1681 ttggatcaca caaactcaaa agtatctcca tattatgcat tattgacaaa agttgcacaa
     1741 gtggacataa attataagaa acagggaatg gtgaaagaat cagaatttct gagagattta
     1801 gaattactac agtatgatat aactgaggga gagggataca tattaaatta tccagatttt
     1861 tttgaattta aataaaggaa gattatatga aaagtaagtt tttttcacat aagttaaaat
     1921 tatttgtttt atctattctg ataataatgt gttcacagtt attgctgagg atgccaataa
     1981 atacaaattt taatcctcta ttagtgatat taattgctat atcaggacta tacttttttt
     2041 tagttgatct gtctgactct gagtctcttg ctaagaatgc tctgattacc atcctgcttt
     2101 ttggtacagt tatttcacta gctaaacctg ttcaatttgg tcttgacgaa gaaacgcatc
     2161 taagaaatac attaagactt tcagatggat ttattattca gtttgagaag gaaaatattc
     2221 cggattattc ttcggtgtat aatcatgaca ttttaagaag tccggacaca tatactgaca
     2281 atacttattg gttaaatgtt gaacatcagg atagtcaatt tagtggaaaa attattgggt
     2341 tgaataatat tgtttattta cctagcgcat taggttggaa tttaggaaaa ctgatttcga
     2401 ccaaaatttt tatttcatac tatctcggaa gaattgctaa tataatagtg ttcgggctat
     2461 taatttattt ggcaatcaga tggagtagag tttataaaga aattgtatat ttgtttggta
     2521 catttccagc aattctctat atttgtgcag ggtttcatta tgactatctc tattatgcgg
     2581 cgtcattaat gttagctgca ttactaacaa atattttaaa tcaggactca cctcttatag
     2641 ataaacaagt tatagcttat ctatcgctga cattcttatt tacttttgca aagtttcctt
     2701 atatactttt aggcgcatta atctgtttgt tacctaatcg atattatcag tcaatgaagc
     2761 aaagacttag aacgtgtttc tatttcagtt tgcaatttat tttatcattc gtatattaca
     2821 taaatggatc aattatcaga agaataacag gtggagttgt agaatctatt ccttctgaga
     2881 cctccctagg ctactttctt acacatccgt ccccagcaat tagaacattt tttataagta
     2941 ctttgagtgg aagtttgaat tcatttgcaa gagcgttaga atatacaatc attgaatcag
     3001 aagtcttaca aagtatttca ttaattctat ttttattttt aatcttttta cttagtttga
     3061 aattaaaaat aaaggtttcc aatatgacaa aaattttgat attcactttc tttgttggga
     3121 ttacattatt aataatttat gctatatctg gtgatgttag agtgtacaga cctggtgata
     3181 tttggattgg gggtgtacag ggaagatact attatctcat gttgctattt ttaccaatta
     3241 ttctttctcc atttttaaga gaaatgctaa tgaaagacga ttatgttatc gatccagata
     3301 ataacgtggc aaaaattatg caacattcaa tcgcgctttt aaatattctt actatagcaa
     3361 ttgcgatttt tacacaaacg ccacataaat tataacttgc ttaagaaaac aaagttagga
     3421 atatttacgg attcgtgagt ttaattatta gaaagtagaa atgaaaagtc caaaagtttc
     3481 aattattata ccagtatata acgctgctaa tacagtcaaa aagtgcgtta gttctatatt
     3541 aggtcaaacc tttaaagatt ttgaacttat acttataaat gatggttctt cggactcttc
     3601 cttatcaaaa atacaaaaat ttatgtttga tagtagggtc agagtaattg ataagcaaaa
     3661 tgaaggggca tcgcaaacaa gaaatagagg cattcgtgag gctaagggag actatttatt
     3721 atttatagat agtgatgatt atattgataa agattatgtg gaatgcttgt ataaggaaat
     3781 tgagcaatca aaatttgata tgattatcag tggtattcga atggttgata gttctgggaa
     3841 agagttaaat cattttcggt tgaatgattc tccatggtcg aagtacatga ttacatcacc
     3901 atgtacacgg attattagaa ggaaatttgt attagagaat aatttatttt tcatagatta
     3961 taccatggaa gatattcatt ttaatgcggt atttttctca cggacagatc gggtaaaagt
     4021 gataccatac attggttata attatctaat gaatcctgag agtactaccc acacgcttca
     4081 taaaggaatt agtaaggaag tcgacattct taacattatt tcctctatac atactgctgt
     4141 caatccgaca gatttagtga aatattttta tagaaaagtg tacatatatt atttactaaa
     4201 atctggaaga tattccacac cggaagtgtt tttaaatgaa tatgaaagaa ttgtagatta
     4261 cataaaaact aatcaactag agtcaacaat tcgattattt gataagcgaa ttagtgaaga
     4321 gaaattcact acaaaattca ttatttcaac atttggtgtt tttgaaaagt ttgacttaat
     4381 gtcattattt gctaagtgtt attgtataaa aaatagtagg gaagggtttt gataaactat
     4441 gtcaattttt gttattactc ataaaaaatt tacacctttt acagaggagt taaactataa
     4501 aatcatgctt gttggtgcga acaataataa aggggaggcg ttttatttaa aagataactt
     4561 tgaagaggat aatatttctg ataaaaattt ttcgttttgc gaactaacag gagcatatag
     4621 tttatataaa cactgtactg atgatattat aggtttagtt cactatcggc gatatttcat
     4681 gaattcagag ggctttccgc ttagttttaa tgaagctgaa tcaattttgg agaagtacga
     4741 tgttattttg cctacaaagt caaagttaac aagttataaa attcctgttt cagtaaaaaa
     4801 acattttatc agcttgcttg gcaaagatgg taaagaagcg tgggaaaaat gttcagatat
     4861 attgaaaaga aaatatccgg aaatgatagt ttcattcgaa aactttgaac gtcagaattc
     4921 tacttatttt tataatatgt gcgttatgaa cagtagagac tttaaggctt atcatgagtg
     4981 gttatttgat atattgttac aacttgataa agagattgat gtcacaagtt attcaaaata
     5041 caatcagcga atgtttggtt ttatttcgga aagactatta actttttggg taatgtataa
     5101 caataagaaa gtaaaagagt tacctatcat gttcacagag ccaatttcat ttttgaatca
     5161 agttaagcag tatgttgaga gaaagttgga taagtgatta atgacatctc gaactaaatc
     5221 cataaaacga aattttatat taaattttat ttttactgtt tcagggattg tttttccact
     5281 cttggctttt ccttacgctt ctcgtatttt acttccagaa ggaactggaa aagtagcctt
     5341 tgtatcttca atcgtatctt attttacaat gattggaatg cttggagttc cagtctatgg
     5401 aattagggct tgtgcaaaag taagaaatga caagaaatta ctttcaaaaa ctgtttttga
     5461 aatatttatt ttgaattcaa ttactatgct attgtcctta ttattatatt ttataacaat
     5521 tgtttcacta ccgaggttgt ctgaattaaa aactctattt tacataaatt ccttgacatt
     5581 gatattgaat ttatttggtt ttgactggtt gtataaagca ttggaagaat acgagtttat
     5641 cacaaagaga acggtattaa ttaagtgttt cgccctcatt ttactatttt ttcttgtcaa
     5701 agaaagtaca gattatacag agtattctat tatattgatt atggcgagta caggttcgta
     5761 tgttttcaat tttttaaatt tacgtaaggt aatcgatttt tctaatatag gcaatctaaa
     5821 attcttccat caccttaaac caatgtttac ttttttcatt atggcagttg caactacgat
     5881 ttattctagc ttggatgcta ttatgttagg ttttatttcc ggagatcaat ctgttgggta
     5941 ttacaatgca gcaattaata taaaaaatgt tttattaagt atgattactt cacttggggc
     6001 tgtattattg ccacgtcttt ctgtatatgt gcaacaacaa aaatttgatg aatttcggac
     6061 attaacatta caagcattac aatttgtagt gtatacttcg attccgttaa cattttattt
     6121 tattattttt gcagaatcaa gtatttactt tttatctggt gtagcctatt atggctctgt
     6181 actacctatg caagttgtta tgccaacgtt attcttagta ggtatatcaa atttaattgg
     6241 aattcaaatt ttagttccat tagataaaga aaccgtagtt gtaaaatctg tatgtatagg
     6301 ggcagtagtc aatttgataa taaatgtgct tttgattcct atttttggcg ctgtaggtgc
     6361 tgctgttggg acattagttg ctgaaatctt tgtcactgct tatcaattat actatcttcg
     6421 tacgttctta aaagatatta ttatccaaat tagatggaag aatattttga tttcaacagt
     6481 attagcaagt atttcggtct atatttttag agaagtaaat aattttgatt caccgttaat
     6541 tgttttgata ctctctttta ttatttttgg tatgatttat ggaattgttc ttattatgat
     6601 gaaagatgat gcattgatac tactcgtgaa aggattaaaa tcaaaaaatg aaaaattatg
     6661 attatttagt agtcggtgct ggtttattcg gagcagtatt tgcccatgaa gcagccaaaa
     6721 aaggtaaaaa ggttaaggtg attgaaaaac gtgaccatat tgccggtaac atctacacca
     6781 aagaagtaga aggtatccaa gttcatgagt atggcgcaca cattttccat acttctgaaa
     6841 aggaaatctg ggactatgtc aaccaatttg cagagttcaa ccgctacacg aacacaccag
     6901 ttgccaacta taggggtgaa atctacaacc taccattcaa catgaatacc tttaacaagc
     6961 tttggggtgt agttactcct gctgaagcgg aggcaaaaat tgcagagcaa cgcgctgttt
     7021 taggtggcaa aacacctgag aacttggaag agcaagctat ctcactggtc ggtacagata
     7081 tctatgaaaa attaatcaag tcttatacag agaagcaatg ggaaaaacct tgtacggaac
     7141 taccagcctt tatcatccgt cgtttaccag tgcgtttgac ttatgataac aactatttca
     7201 atgatactta ccaaggtatt ccaatcggcg gctacacgca gattgttgaa aaaatgcttg
     7261 atcatgaaaa tattaatgtg gagacaaatg ttgatttctt tgctaataag gaagaatact
     7321 tggcgaacta tccaaaagtt gtctttacag gtatgattga tgagttcttt gattatgagc
     7381 ttggtgaatt agaataccgt agtcttcgtt ttgaaactga agtcttggat atggaaaatt
     7441 accaaggtaa tgctgttgtt aactatacag atagtgaaac accctttact cgtattatcg
     7501 aacacaaaca tttcgagttt ggggcacaag ataaaaccat tattactcga gagtattcga
     7561 agacttggaa acgtggggac gagccttact atccagtcaa taatgaccac aacaataaac
     7621 tctatactgc ttacaagcgc ctagctgaac aacaagaaaa tgttatattc ggcggccgtt
     7681 taggccacta tcgttactac gatatgcacc aagtaattgg agcggccttg cagtgcgtga
     7741 gaaatgaaat gaagtagttt gaagtaaaat attaattatg taagttttta gtgagaaaac
     7801 atgaaaatac taatgattat ccctgcctac aatgaagaag aaagtatcct tcaaactgtt
     7861 cagggaatta tagattatag aaatagtgtg aatttccaat tggattatgt tgtcattaat
     7921 gatggctcaa cagatcacac gaaagaaatt ttgattcaaa ataaattaaa tgctgttcat
     7981 ctagtacaaa atttaggtat tggtggagct gttcagacgg gatataaata tgccttggac
     8041 aatgactatg acgtagcagt tcaatttgat ggtgatggtc agcatgatat tcgttcatta
     8101 aatagcttaa ttcagccaat cttagttggt caggctgata tggtgattgg ctcacgcttt
     8161 gttggtgata ctttgtcgga atttcaaact agctttatga ggcgttttgg aataggtgtt
     8221 atttcaaata tgattaaatt aacaactgga aaccgaattt gggatactac atcaggttat
     8281 cgtttgggaa atagaaaagt gattgcacaa tttgcaaaac gatacccaat taaatatccg
     8341 gaaccggaat caactgttca tttactaaag caaaatttcc aagtagtaga agcaccggcc
     8401 aatatgtttg aaagagcggg aggggtttct tcgattactc catttaagtc catccgctac
     8461 atggtagaag tttgctcatc tattttgatt gcttcgctta tgaaggaggg ggaataatga
     8521 ctatacaagc attagctatg tttcttgcct cgctatgttt tttgtatttt atttttagga
     8581 atattaataa aaataagatc ttattcgaac atgcctttat gtggattgtt attggcttcg
     8641 gactgattgt ttttgcgctc tttgatgtta ttcctattaa actggcttac ttattcgggt
     8701 ttggtctgac ttccaatttc ttgttatctg tggcaatttt tgttctcttg gtgattggat
     8761 ttttgcatag tatggcttta tctcaacaaa agcagcagat taaaaatctt attcaagaag
     8821 tttcaatggc taaaaaaaga attgctgaaa tggaggaatc tgatgcagaa taatcatacg
     8881 tttgttatct gtgcctatgg tgatagtaga tatttggaag aatgtatcca gtctttggtg
     8941 agtcaatccc taaaaacaaa tattattcta tatacaagta ccccaagcga ttatattaat
     9001 ggtctttgtg aggagtacca aattccatat tattcttccc ctggaggagg tattggaaaa
     9061 gactggaata atgctttgtc tttcgtacaa actcaatttg caaccattgt tcaccaagat
     9121 gatgtatatc tgaccaagta tgcagaaaaa atattgtcat atatgactga cgaaaccctc
     9181 attgcttatt cggattatca agaagtgaaa gagggtgagg tcattgccct aacttcaaat
     9241 ttgaagatca agcaattgat gttacggact ttggatattt ttcctaagtg gaagttttgg
     9301 cgtaatagag tgttagcctt tggtaatcca atttcttgtc cagctgtgac ctataatttg
     9361 aataacttga aggatttcaa gtttaatgaa gaaatgaaag tttctctgga ttggttcgct
     9421 tggtatcaaa tatctcaaaa aaagggtaga tttacctttg tagatgagag tttgatgtat
     9481 catcgtatcc atgaagaatc ggagacgaca aatagcattg aaaacaatat tcgtacaaaa
     9541 gaagattatg aaatgtattt gttattctgg ccaaccttta ttgctaagtt tcttttgaga
     9601 tactatgtca agagtcaaga tgcaaatggg ggaaacaatg gggagaagta agactgtaac
     9661 caatacacga actcgcaaac gaacgtattg gtggtttaat ttgtatgtag tagtgttgat
     9721 aggcttacta gtcggctaca agcatttttc tttgttgcga gatggttata tcccattaaa
     9781 tacagtttta gaaaagcaat catttaaaaa gatttctgat tggcagtatg agaaaaaaat
     9841 cggtgattta tcttactcct tgctgattga tacagataaa aacagcgtaa caaaagcggg
     9901 gtatcaattt gatattcgta ataatattca acatttttta tggatggatt atctatcagc
     9961 agataagatt gaggaaatat tcaatgttca agtttcttcc agtggaattt ttgtagatgt
    10021 taaagatatt gaattttcac aacatcagtg gacagaggaa tttccaaatc ttattgcaca
    10081 tgctggaggt acttatcgtg aaaaagcata taatacattc tatatgaact ccttagaggc
    10141 tttgcagcag aactacactt taggtcatag ggtatttgaa cttgattttt atctaactag
    10201 tgatggaaaa atggctgcag tgcatgattg ggaacaattt gggcatatga atggcctagc
    10261 attaagttcg gaagaatgga aaaactttca aacctttggt tcaccagtta ctgaatcacg
    10321 ctttacaact atgcttattg gtgatgtgtt agatcaaatg ctgataaata aggatatgtt
    10381 cttagtgacg gatactaaat cttttgaagt ttcagaagaa gaagtaatcc atcagtttac
    10441 tgaaatttat aatgaagcaa tgaaacgaag ccctgaatta ctaagtcgta ttattccaca
    10501 aatttacaat caaacgatgt atactaccct aaaaaaagtt tatgatttct caaatgttat
    10561 ctacacgcta tatgtctcgc ccgatagtcc ggaacaggtt attgaatttg tggctaataa
    10621 tccatctatt aaagtggtta ctattccttt gaatcatggt ggatatttta attcagaatt
    10681 ttttaataac cttcatgctt tagataaaaa aatctatact cataccattc acacatatga
    10741 tgaattgacg aaatactctg cgcttggaat tgatggtttt tataccggtc tattattgcc
    10801 aagtgatatg gagagattat caagtttgag gtaatcttta ttattactga tggcactatt
    10861 ttaatcatat catcaaaaaa tatttcaaaa gcatttcaga agttttacaa accaaccaat
    10921 attgtcatgt aaatgtaata aaaaatttca aaagatagag gttttcatag gaaaatctct
    10981 ttttttgtgc tagaataata gatggaggtc cgatatgatc aaaatttttg gtaaaattcg
    11041 ttaccattgg caaccggatt tatcatgggt cattatttac tggtcactta ccttcacgcc
    11101 tattttcatt ggtatgacac ttttattgga aaagttgaga gtttcaagat tatttattct
    11161 acttgtctca ctctttgctg tattagtagt gctcggctta catcgctatt tcgagataaa
    11221 agatgagcat ttacggattg cttcggctaa tccttttgca gtgctgaaaa ttgaaattgc
    11281 taccatagaa aaaattgaag tttcttatct agccattcgc atattttcac aagaatttcc
    11341 caatgggcag gtgtatcaca tgcgcaaatg gccgaaaaaa tactttatta accatttagc
    11401 tattcataat tgcttcaagg gtgagattga attaatagac catttaataa aacaggatta
    11461 ttttgaagag tattatacaa aaaaagccaa atcaatccgc tgaaatggct tttgtaggac
    11521 atgatttgat ggctttaaga atatctggat tgggttcaat cgttttctca aattgtgatt
    11581 cgtctgaaaa aacaacgata ccgtcgccat cataatcgaa tagagatgag taggtttggc
    11641 agagcccgca agcaatgcac cgttcaggaa taagttttac tttcatatta gtattgtaat
    11701 catattttaa taaaaataca aggagttttc tcaataatgt cacaagagcc atggaatgaa
    11761 gaagtttacc acacagaaac aactagtcgt aaagaacgtc tgagtaaggg ggtagcgaat
    11821 acaacagtat ttacagtttt ggcaattatt ttctttgtta tcgtactgat tattggaatt
    11881 atggctgtct atctctcaat cggtggcggt agtaaaacag attcaacaaa agaattttat
    11941 accccaagca ctacaacagt cgtagcagag agctcttcat cggctgctga gacacaagaa
    12001 tctacttcga ctgacacgac agaaacagaa acaagttcca gcccagcgga agcaacggat
    12061 ggctctaccc ttactgttca agcaggtgaa ggtgtaggac aactggctgc tcgtggtgga
    12121 atttcaattg cagagctaga gcgtttgaat ccagagaaaa tgactacagg gtcttggatt
    12181 gcgcatcctg gggatgtagt tcgtattaga taaagaggag ttatgaaatc aattcaaatt
    12241 gcaattgacg gtccagcttc aagtggaaag tcaacggttg ctaaaattat tgctaaaaat
    12301 ttgggatata cttacttaga tacaggagct atgtatcgct cggctaccta tctagccttg
    12361 acaaacggca tagaggtcac agatcaaaaa agaatcgtgg ccttgcttgc ccagtatccc
    12421 attcgatttg ggcgtgatga aaacggtcag caacttgttt ttgtcggtga tgaagatgtt
    12481 actttgccta ttcgtgataa ccaagtaaca aataatgtat cagcagtagc agcgcttcct
    12541 ttagtacggg aagaattggt ccgcctgcaa caggacattg cgcaagctgg tgggattgtt
    12601 atggatggtc gtgatattgg tacagttgta ttgccacaag cagaactcaa aatattcctt
    12661 gttgcctctg ttgaagagcg tgcccttcgt cgatttaagg aaaatacgga acgtggcatt
    12721 gagacagact tggaatcctt gaaagaggag attgcggctc gtgactataa agattccaat
    12781 cgagaagttt ctcctttaaa ggcagcagat gatgccatta cctttgatac aacaggagta
    12841 tccatcgaag gggttgttca atttatttca gaaaaagcga aagaaattct tgacaggtag
    12901 ctttagacat gttagaatag tcgtattgag aaaagcagaa gtgagaactt ctcgccttgc
    12961 gactaacgtt gtctggctct acggattaag atttcttcgg aaagaataat ggtgtagtgc
    13021 ggacttgttt agcaagtccg tttcgttttt ctcgaaaaat ataaaatgag gtgaaaagcc
    13081 atagcaaagc aagatttgtt catcaatgat gaaattcgag tgcgtgaagt tcgtcttatc
    13141 ggtcttgagg gtgaacagtt gggaatcaaa ccgctcaatg aggctcaggc cattgctgat
    13201 agcgccaacg tggatttagt attgattcaa ccccaagcta aaccaccagt agcgaaaatt
    13261 atggactacg gtaagttcaa atttgaatat cagaagaaac agaaagaaca acgcaaaaaa
    13321 caaagtgttg tcactgtaaa agaagtgcgt ctaagtccag ttattgataa aggggacttc
    13381 gagacaaaac ttcgtcatgc ccgtaaattc cttgaaaaag gaaataaggt taaggtttcg
    13441 atccgtttta agggtcgtat gattacccac aaagaagttg gagcgaaagt attagctgag
    13501 ttcgctgaag caacccaaga tattgccatt atcgaacaac gcgctaagat ggacggacgt
    13561 caaatgttta tgcaattggc cccagcttca gataaaaaat aagcttttta agcaaaagga
    13621 gaattaacat gccaaaacaa aaaactcacc gcgcatcagc taaacgtttc aaacgtacag
    13681 gttctggtgg attgaaacgc ttccgcgctt atacttcaca ccgtttccac ggtaaaacta
    13741 aaaaacaacg tcgtcacctt cgtaaagcag gtatggtaca ttcaggtgac ttcaagcgta
    13801 tcaagtcaat gcttactcaa atgcgctaag cactgatata gcactactta ttatttgaat
    13861 tatttggagg aaaattaaat ggcacgtgtt aaaggtggcg ttgtttctcg taaacgccgt
    13921 aaacgtattt taaaattagc taaaggttac tatggagcta aacatctctt gttccgcact
    13981 gcgaaagaac aagtaatgaa ctcttactac tatgcatacc gtgaccgccg tcagaaaaaa
    14041 cgtgatttcc gtaaattgtg gatcactcgt atcaatgcgg ctgctcgtat gaacggtttg
    14101 tcatactcac aattgatgca cggtttgaaa ttggccgaaa tcgaagtaaa ccgcaaaatg
    14161 cttgctgact tggcagtgaa cgatgcagca gctttcacag ctcttgcaga tgctgctaaa
    14221 gcaaaacttg ctaaataagt ataaaaatgt aaagaacttg gtagtgatga accaggttct
    14281 ttatttttgg aaagtagctc actacttgct aaatttcgat atgagtgata tacttaacta
    14341 gtaaataaat cgacggtttt aactttatcg tcatttagtt tttcttttat tacttcgacg
    14401 aatgaggaaa ctttgtttcc ttatttccaa cttcaaacac tccactggag tgtttgaact
    14461 cgccttgcct aacttcagtt atgcctgtgg ctgttgacac aaacttcgtt tgtttcattt
    14521 ccgaccttta aaggttcccc gaacctttga agctagtaat aaaagtaaac taaaagacta
    14581 taataagtgg atgtggtgat gctattttac aatagttgat tacatttatc gtgtcggtaa
    14641 tataataatg gtaaataagg agagaaacat agatatgaaa caaggtaaga cttttcttta
    14701 tgtagcagga gtatcgctta tttgtgctgt agcattggtt acttatcaag cgtctaacta
    14761 tagagagcaa tcgaaccatc aaaaaacaac aagcatagaa acatcgcaat caaagcaatc
    14821 aaaagagatt ccttcagatt cgaaaaagga agttccaggg attgataagg cgacggatga
    14881 tggtttttta ctcactgatg agtcacaaat tgaggaaaaa actgatttag gaattattgt
    14941 caaccatggc gatcataagc actttttctt ttactcagat ttaaagaata caaaatgggc
    15001 ttatttaatt cctgaaaact atcaagaaaa aaatcattct tctcaaccta agagaagtca
    15061 attatcgtca ggctcgaaac agatcgaaga tgaatacgtt tttgatccta aagatattgt
    15121 cgcagaggat gccaatggct atactgttcg gcatggggat cattatcact atattttaaa
    15181 atcaagctta ggtggcacga cacacagaca attgacctct gatagtcgtc aatcgccaac
    15241 tgttcctatc gttcaccaac aggaagggat tccggggatt gattttcaaa cgagtgatgg
    15301 tttcttgttt gatgggcaaa atatcagcgg tgtaacggaa accgggattc tagtaaggca
    15361 tgggaaacat ttgcatctta ttcattttga aacattgaaa aggagtaaat ggtcttatct
    15421 tgtgaatcag tataaaccaa atgttgaatc ggataagaag tcacaatctg aggtagaaaa
    15481 tactgagtat caaatgaaat tggactactt agcaaaagaa ttgaatttag agccatcacg
    15541 tcttaagaaa gtcatcgttg acggtcaaat tggattagag tatccgcatg gagatcacac
    15601 acatattgtt ttattaaagg aaattgatac aacgaagccg tttgaaagtc cagaagatcg
    15661 tattcttaag caaaaggatg gagaaactct tgaacagaga aaagaacgtc tcattaagca
    15721 gtacatggag cgttttaaag tcaagcgtga ggacattaca attgacggga actatatgag
    15781 tgtacgccat ggggaccatg cgcacgttta taaaattgat cctagtttgc cagatgatcc
    15841 tgagcgcgat gttaagaccg aaacggtaaa tcttgctatt gagaaacaat tagtatatgg
    15901 acctttttat acagaaggtt caacagaaaa tttaacacgt aatggtgtcc atcaaaagta
    15961 tcatccggaa ggtcttcagg atattaaaaa tttcattcta gtaacattca gtacaaatag
    16021 tgattttggt gattttgtcg ttaatggtaa aaaaacgaag agagtttatt atttggttcg
    16081 taaagattta aattggaagg atttgaacat cgcctatccg cagacccttc aacaaaaggg
    16141 acgcgtcttt aacggttgga atgctacttt gccaaagtct ggcaagatgg caagagaaca
    16201 tcaaagtttt tatgctgatt ttgataatat ttatcgtaag ccaaccaaaa atatttacac
    16261 tccaagtgat gatgtttcaa atattgattt atcagactat gtacctgtta aatatagtgc
    16321 gatagccaat ggtcgtttaa aattaaatgg tgagattcga gcagggttca tatactttgt
    16381 gaaatctgac ttaacttgga aacaagctaa ggagcaggga cttgttgtac cagaaccggt
    16441 tccaagcaaa gattatgagt tcattgagtt taggacagtt attactggtg gagaaaaaga
    16501 tacagattat gttagtgcca cagcgagctt ggcagcattt gggacaacgg ctcctcgtat
    16561 aggaccttac attgctaaca atacggagaa tccaacggac atcaatgacc caagtagaca
    16621 tccaaattac tattggcatg atccaaagaa ttatgtagcc ctcgcatttc gagcaggtga
    16681 aggcggtcaa cttcagacac atttgggaac tagtaaaaca gttgtttacc ttgttagaaa
    16741 agggttaaca ctgaaccaag ctggaatttt tccgccatct ttgaagagtg atacaggtta
    16801 taaacgagat tatacaaaac ctgttattcc aaatgtagca tgggatacac ctattttgga
    16861 agatactgtc tacgatattc atttcgataa agttgaaagt gatagcaaag atgggacagt
    16921 tgagccaggt gatacttggt tgcctgggaa ttcattgccg aatactccag aagttcctaa
    16981 tggagaagag aacgcagata gctggttaga tgatttgttg acacctagtc cagaagcgac
    17041 agatgtagaa actgtaacag aagctgaaac tacaacagaa gatacaaaag caactgaaag
    17101 tagtactgct gcccccatag atgtccctaa aaaatcatca acagaggaag agcttagtga
    17161 ggattctata tttccttgat gaaatagata tgagtgattt gaaatagtgg tctaatactc
    17221 ttcaaaaatc aaaatcagac gttgttgagt tgatttgatg agtgaaaggc tccagtggag
    17281 cctttcagcc tgttaccttg aaacaaaata gtaacagggt tctatctgct aagtagaaac
    17341 gaactacgtt cgctctatct ccaacctcca aaggttcccc aaacctttgg agctagtctg
    17401 tttttgattt tcattgagta taaaaaggct tagaatcaat gtagtaactg acatgattct
    17461 aagcctttta gtgtattacc gtgaatttga actggtactt gttgagctag tgcttgtacc
    17521 agtatttggt gtactagtag cagttgtact accgtgaagt tctaggtagc ttggtgcttt
    17581 aaatagttca attgttgatt tattgccgtt tttactatac aaacttgtcg attgtgtacc
    17641 aagttttttc tccagtgcaa tctcggcagc caatgagtca atgtagttat attgacttga
    17701 atccaaggct ggcaatccat tgtcgaagaa ccggataaga tcgccagttt gaatggcatc
    17761 gctagtcgct agttgtgctg cggtagcttc tttaattgca tcaatttctt tctgagttgt
    17821 ctcatctgga tttgtaattt caagaccagt ttctgtatag tagattttac cttcgtagct
    17881 agtgtatttt ggtgtgataa aactgccagc agttcgaaga gccacaattt gactgtgttg
    17941 gctggaaagt aggtcttgac caacttgaag atagttatcg gatttgattc ctaaaaggtg
    18001 ttcgagagta ggaagtgcat caatctcacc accgtaggta tcaaaaatcc caccgttggt
    18061 cattccaggt acaacaatca tatatggaac acgttgcatc atggcattgt caaaatctga
    18121 ccaagtctgc gaatcttttc caatcaacgg tgccaagtca ggattgcgtg aattagaaat
    18181 tccatagtga tcaccataga gaacgatgac ggagttttcg tacaagccag tcgcttttaa
    18241 gtaatcgaaa aatgccttta tggacgcatc aagatagttg gcagtagcaa aatagccatt
    18301 aatggtttca tcatctgtat tggctaatgg aaagccaatc tcatccccaa tcaagctggt
    18361 tgtatagggg tagtggttgg aaactgtgat aaatttggta tagaagggtt gctgtaaatg
    18421 ttccagatat tggatagagt cggcgaacat gattttatca ttcagaccgt attggaatga
    18481 attttcagca gtggctttag agaaatagga ttgatcaaag aagtaattgt aaccccattg
    18541 tttataggtg ttgttcctat tccagaaact ggctgcgtta ccgtggaata cagcagatgt
    18601 atagccacca ttttgagaga gaatatatgg tgcggcttgc tgggtattgc ttccaccata
    18661 ttggaccatg aaagagcctt gattgagtcc aaaaagcgag gtttcaatca tggtttcggc
    18721 atcagaggtt ttacctgcct tcacttggtt gaagaaatta gaaaaggcga gagtagagct
    18781 agaatgatac agagaattga ggaaaggagt gacttcgtat tcgacaccgt tgacgttcaa
    18841 cttatagtca ataacaaatt gttgtaaact ttctaggtgg agatagataa catttcgtcc
    18901 tttggcaata ccgaagtagt tgtcattcgg ctgagcataa tgttctgtaa tatattgttt
    18961 cacaggctcc aaatctgtag ctgatgctcc agaacgattc ttgtgggcat tgtaggtttg
    19021 attagcattg taaccaagga aagcaggtaa accaagggct ctgacgatat aagtattaga
    19081 aaatcctctc gtaagtagct ctggtctatc aatttctgct aaaaagaggt tgatggagaa
    19141 aagcatgact gatagtgctg taatagcaaa gctagatttt ttatgaaatg tctttttatc
    19201 ccaccggagt agtttacgat agactaggat tccaaaaatg ataaaatcaa gaaaatagac
    19261 aatatcccat agcctaaata acttaagagc tgcttctcct aatccagcag atacactaga
    19321 ggtagccatt atagtagaga tggaaatata atcggaaaat tctctgtaat agactgcatt
    19381 tgaaaagagc cagacggaaa gaataatata aatgatcatc gctacgctat agaagacttt
    19441 tgtattcttt atataaagcg agagaccgag taataaaatc ccgactggta gaggattcat
    19501 aatagcaata aaattttgga aaaacccttg tgtatctaag ttgaaatcaa catagtaggc
    19561 ccagagtgtt ttgagccagt aaatgattag caaaatcaag acaaaaccaa gtctagtctg
    19621 tccgctcttc ttgacatagt caattagttt tttcacaata aaacctcatt gtcctttcta
    19681 aaagtttagt tcattatacc ataattctaa aataagcctc aaagtttaga gtttttttgg
    19741 catatatgaa gaaacatgtt ataataattc tatgaaaata aaggtaaacc gtattgcaga
    19801 acagaaaata aacagaggga ttcaactctt ggatagcgct gatttttcag gtttttcgtt
    19861 tgatgaggac aggctggctg ttttgtattc agaatctgga agctatttgg gccaggctta
    19921 tttgtccaaa cagaataagg gaataggctg gctgtttagt agaagcaatc aagacttgac
    19981 caaggatttt tttgttaaat tattcgtgga tgctaaacag aaacgtcaac atttttatca
    20041 atctgagatg acgaacgctt ttcgtttatt taatcaagat ggagatagtt ttggtggaat
    20101 gacggttgac ttgtatgatg aatacgcagt tttttcatgg tataatacct ttatttattc
    20161 tgtcaaagag attattgttg ctgcatttca acaggtattt ccagaagtaa gaggagctta
    20221 tgagaagatt cgttttaaag gtttggcctt tgagtctgct catctttatg gagaagaagc
    20281 ccctgagtat ttcactgtgt tggaaaatgg agttcactat caggttttta tgaatgatgg
    20341 tctgatgacg ggaatctttt tggatcagca tgaggtacgt ggttccttgg tggatggcct
    20401 ggcggcaggc aagtcacttt tgaacatgtt ttcgtatacg gcagcatttt ctattgctgc
    20461 tgccatgggc ggagcagggg aaacgacttc tgttgactta gccaaacgct cgcgagaact
    20521 ttctaaagct cactttaagg ttaatagttt agagttagat aatcatcact ttgtggtaat
    20581 ggatgtattt gaatatttca aatatgccaa acgtaaaggc ttgacttatg atgtgattgt
    20641 cttggatccg ccaagctttg cacgaaataa gaaacaaacc ttttcagtag ccaaagatta
    20701 tcacaagttg atttctcgat ctttggaaat actaaataca ggtggaacta ttatcgcttc
    20761 gaccaacgcc gcaaatgttt ctgttgaaaa atttaagagg cagattgaaa aaggttttgg
    20821 tgttagaaaa caccgttatg aggcaagtta tcgcttgcca gccgattttg taatcaataa
    20881 aaatgatgaa agtagtaatt acctaaaggt ctttacgata caggtagata aatgaaaata
    20941 gtagttccta ttatgcctag aaatctggaa gaggtagagg ccattgatgt agaacgtcta
    21001 gcagaggcag atattgttga atggcgagca gactcccttc cgaaagatga tattttaaga
    21061 gtagcacctg ctatatttga aaaatgtaat ggtaaggaag tggttttcac cattcggaca
    21121 acacgtgaag gtggccactt ggatttggac gatcaagaat atgtcaattt aatcaaagaa
    21181 gttgcagcgc tttatcagcc agattatatt gattttgaat attattctta taagtcggtt
    21241 tttgagcaga tgttggagtt tcctaatctt gtgttgagtt atcataattt tgaggaaaca
    21301 ccgtctaatt atatggaaat catgtctgaa ctgactagcc tgtcaccagc tgttgttaaa
    21361 atggctgtaa tggctaagac agagcaggat gtactggatg ttatgaatta tactcgtggt
    21421 tttaaatcgc tgaacaccga gcagacattt gcgacaatcg ctatgggtga actagggaag
    21481 ttaacacgta ttgcaggtgt gattacaggc tcctgctgga cttttgccag cttagatgaa
    21541 acatctgcac caggacaaat gtctttaagc aatactcgta agtttctgga aattttggag
    21601 aattaagtta tgcgttattt aacagctggt gagtctcatg gaccacgtct aacagccatt
    21661 attgagggag ttccagcggg tcttccttta acagccgagg atattaatga agatttaaaa
    21721 cggcgccaag gtggatacgg tcgtggtagt cgtatgcaga ttgaaacaga tcgcgtagag
    21781 attacagctg gtgttcgtca cggtaagaca accggtgccc cgattacatt gaatgtgacc
    21841 aacaaagacc atcaaaaatg gttggatatt atggcagttg aggacatcga agagaagctc
    21901 aaaagtaagc gtcgtatcaa acatccacga ccaggacatg ctgacctagt tggtggtatg
    21961 aaatatcgct ttgatgattt gcgtaattcc ttggagcgtt ctagtgcgcg tgagacaact
    22021 atgcgtgtgg ctgttggtgc ggttgccaaa cggattttgg ctgagttgga tattgaaatt
    22081 gctaaccatg tagtcgtttt cggagggaag gaaattgatg tcccagatgg tttaagcgta
    22141 gcagaaatca aagagcgtgc agcccagtca gaagtatcca ttgtcaacca agagcgtgaa
    22201 gaagaaataa aagcctatat tgatcaaatc aagcgagatg gagatactat tggcggagtg
    22261 attgagactg ttgttggagg agttccagct ggcttggggt catatgtaca atgggataaa
    22321 aagttagatg caaaattagc gcaggctgtt gtttcaatca atgcttttaa gggagtagaa
    22381 tttggagtag gcttccaagc tggttatctc aaagggagcc aagtcatgga cgaaattctc
    22441 tgggcccaag aaaaaggata tacgcgccga accaataatc taggtggttt cgaaggtggt
    22501 atgaccaatg gcgaggctct gattattcgt ggtgttatga aaccaattcc gaccctctat
    22561 aagccattga tgtcggttga cattgatact catgagcctt ataaagctac tgtggagcgt
    22621 tcagatccaa cggctctccc agcagctggt gtagtcatgg aatcagtcgt agctacaact
    22681 ctagcgacag aaatcctaga gaagttctct tccgataata tggaggaatt gaaagctgca
    22741 gtagccagtc atcgcgacta tgtcaaaaat ttctaggtaa ttaatgaaaa aaacgatttt
    22801 aattgtaggt ctagggttga ttggaagttc acttgcgctc tgtattcgca aagaccatcc
    22861 tgactatcac ctcattggct ttgatcctaa cgaacaatca tgccaaattg ccttgggcaa
    22921 ggggatggtt gatgaggtat caacggaatt ggagggtgta gcaaagcagg ctgatgtgat
    22981 tcttctttgt gttccaattc aagtttcttt gcgtttgatt gaagaattta gtgcccttga
    23041 attgaaggat atggtcttga ttacagatgc aggttcaacg aagtctgcaa ttgtggctgc
    23101 tgctgaggaa catttggcgc caagacaaat taattttatc ggtggacacc ctatggcagg
    23161 tagtcataaa tctggtgcca gtgcagcaga tctccatcta tttgagaatg cttactacat
    23221 tatgacaccg tgtcaggcta ccaagcagga tgcggttccc cgattgacag acttgttatc
    23281 tggaacgggg gcacgttttg ttcagattga tgcagcagaa catgaccgtg tgaccagtca
    23341 aatttcacat tttccacacg tattggcttc tagtttgatg catcaagctg gtgagtatgc
    23401 tcaagatcat ccctttacga ataattttgc agctggagga tttagagata tgacacggat
    23461 tgcggaaagt gagccgagca tgtggactag cattttgcta accaatgcag attctattct
    23521 aaatcgaatc gaggaatttc aagcacgtct ctcagaaatt tcagatgttc tcagatctgg
    23581 cgatcaaaaa gcaatttggg acttttttga taagggacgt caaactcgta gagccatgga
    23641 aattcacaag cgtgcaggtg ttgattcttt ctacgattta ttccttagtg ttcctgatga
    23701 ggaagatacg attttgaagg ttttggaagt cttacggggt atttccattg tcaatattcg
    23761 tatcaatgaa gagaaccgtg aagatataca cggtatttta caaatcactt ttaaaaacaa
    23821 gggaaatttg gaagacgctg cgaaacgttt aagagagcag acgacttaca aaatccattt
    23881 agattaggag aaaattatgt caacaaatat ctatgatatc gcaaatgaat tggaacgtgc
    23941 aatccgtaac cttcctgaat ataaggcggt ggaagccgtt aaggcctcag ttgaggggaa
    24001 ttcagaagcc aaagaaatat tggaaagtta tatttctttc caaaaagaaa tccaatctaa
    24061 attgcaggct ggtgaaattc ctactgaagc tgaccaaaag aaaatgttgg atttcaacaa
    24121 gaaagttcag ggaaatccac ttttgacgga atatttcagc aagcaacaac agttgggaac
    24181 ttatgtagct gatttggaac gtatcatctt caagcctctc aacgaattgt tgtaatatat
    24241 ttatatttga taggagcagg gtgttaggtc aggtattgac gcctagtata cactgcttac
    24301 gtagagttct tatctccaag tacagattca gaaacggtac ttggggagta agaactcttt
    24361 tttctacctt ctcacagcat ccatttctta taaagttatg gtaattactt gacattttct
    24421 aatagaagtt ctacaatata tttagtatat ggttataacc agtttagcaa atatatatat
    24481 caataaagga gataatatga cagtatttat aaaagcaaaa tctattattt taccagagag
    24541 tgaggtcgaa tcagcctatt tggaaattac tgacaagggt cagtttggac ggattgtgac
    24601 tgaaaagcct gaaggggaga ttgttgatta ttctgacttt catttagcac cagggctagt
    24661 tgatacacat atccacggct atgcttctca cgatgttatg gacaatgatt ttgaagggat
    24721 taaggtaatt tcagaaggtc tcttgtcttg tggggtaacc tcttggcttc caacgacatt
    24781 aacagatacg actgaaaatt tagatgcagt ttgtgaaacg attgggactt atgcagggca
    24841 ggaaacaggc gccaagattc aaggtatttt cttagaaggt ccctttttta cagaaaaata
    24901 caagggagca caaaatccta agtacatgag cgatccatcc attgaaaagt tggacaactg
    24961 gcacagctta tctaaaggtt tagtcaataa aattgctatt gctccagaac gtgaaggcgt
    25021 caaagaattc atcgagtttg ctaacagcaa ggcaattcat actgccttgg ctcacagcga
    25081 tgcgacctat acacaagcgg aagcggcagt agaagctggt gccaatatct tcgtccatgt
    25141 ttacaatggg atgagtgggc ttcatcatcg tgaaccaggt atggtcggag ctgctttgaa
    25201 tttaaaaaat gtctatgcgg aaatgatttg tgatggacac catgttcatc cagctgcggc
    25261 agaaatcgtg gtcaaagcac gtggagtaga ggaaactgtt ttgattacgg actgtatgcg
    25321 agctggcggt atgggtgaag gggagtcccg cctcggtgaa tttgaagttg ttgtaaaaga
    25381 tggaactgct cgtttaaaag aaagtggtag tttggcaggt tctatcttag aattgattga
    25441 agcggtgcag aatgttgtga aatggggctt ggtgtcccta cctgatgccc ttcgtatggc
    25501 ttctctggca ccagctcgct ctgtcaatat tgaccatatt tgtggtcgga ttgcagaagg
    25561 ccgagctgca gactttatcg tcgtagatga tgcaggacgc ttacaagcga cctatctaga
    25621 tggcgtgaaa cgatttggtt aataaaattc cttaggagga aactaatgac aaaattacaa
    25681 ctttctcagg ctaaattcaa tcacatgacc cgcttgtcaa acagcgatca ggtgattgcg
    25741 gccttggcga ttgaccaacg tggtgcattg aaacgcctgc ttgctgctgc agcaggtggt
    25801 ggtgaatttg gtgatgacat tttgattgac ttcaagaaag tcatctcaag cgatttgact
    25861 ccttatgcaa gctccattct cttggatgcg gaatacggtg ttccggcttc agaattgcgc
    25921 catgcagatt gcggtttcat tgcagcttat gaaaaaacag gttacgatgc gtccacacca
    25981 ggtcgtttgc cagacctttt gccaaattgg tcagccaaac gtatcaaaga attgggggcg
    26041 gatgccgtaa aaatcttgct ttactatgat gtagatgaca agccagaaat caacaacatt
    26101 aagcatgctt gggtagaacg cattggtagt gagtgtatcg cagaagatat tccttacttc
    26161 gtcgaaatct tgacttacga tgccagcgat atggatgtga cttctcgtga atacgcagcc
    26221 ctcaaaccac acaaggtcaa tggtgccgtg cgtgaattta gtaagtcacg ttataatgcc
    26281 gacgtgctca aggttgaagt gcctgtcaat atgaactttg tagaaggcta cacagatgaa
    26341 gagccagtct atacagttga ggaagctaag gccttcttca aggaacaaac agacagcact
    26401 cacttgccat tcatttattt gagcgctggc gtatctgcta aacttttcca agaaaccctt
    26461 gtctttgcca aagaagctgg ttcaagcttt aatggtgtcc tttgtggtcg ggcgacttgg
    26521 aaggatgcag tagccatctt tgcaagcaag ggtgaagaag cagccaaagc ttggttggcg
    26581 gatcaaggcc gccgcaacgt agaagaactc aacgaggttc tagcaactac agcgcgtcct
    26641 tggactgaaa aagttgaagt aaaataattg ttttagtagg gtctgggctt aggttcagac
    26701 tgttttgttg catcaaatat agtcatttat actcttcgaa aatcaaaatc agtcgttgtt
    26761 gacctggttt tgcaatccgc tcttatatct ctcaccttga acatgtgcaa gcaatcccag
    26821 aaacagttat agctgcggtt gttgatgcaa accttgttcc ccttatttcc aacctttgac
    26881 tatttgtgaa atagttggag caagtactaa aaggctatat aaacatgatg acaggtcgtt
    26941 ttatagctaa taatataggg gtctattttt agaaaaattc gaaaaaactt gaaaaatctg
    27001 aaaatttacg caattcccta ttgacaaata atttgaaaac agttatgata gttacaaata
    27061 aatgagaaag gaaaacagag atgttacaaa tgtgtcaaaa tcattcagct aatcatactt
    27121 atttctacgg ctattttaga tagtgtttgt agacatggta aacatggatg caaacacggg
    27181 aacgtaaagt ttgtatctat gtggctgtag ctattttgac atgtgcccat agatgataca
    27241 tctaaatggc acggataatt cattctgttt tcctatacag atgaaatata aggaccgttt
    27301 agagtgtagt ctaagcggtc ctttcttgtt tgctattata tttataaaag gagaataatc
    27361 atgggattta aaaaaattat acttagttca gtagtacttt tgtcagcagt aacgcttgct
    27421 gcatgcagtt ccaattcgtc agattcaagt tcagtcaatg ttggtattat tcaatatgct
    27481 gaacatgaag cgttgagctc agctcgtgaa ggctttgtag aggcgctcga agaagcaggc
    27541 tacaaggaag gtgaaaatct gacgattgat ttgcaaaatg cccaaggtga tcaagcaaac
    27601 ttacagacaa tggttgagaa attggcagga aaaaatgatc ttaactttgc catcgcaaca
    27661 ccggcagccc aagccatgct taatgcagat agtgaaacat caagtgtatt cacagctgtc
    27721 acagatccag ttgaagcagg tttggttgaa tcattggaaa aaccagaagg gatcatgacg
    27781 ggatcgactg atgcaactga tgtggaaggt caaatcgaga tgttgctcaa agttgtacca
    27841 actgcgaaga ctgtcggtat tttctacaat tctagcgagg taaactcaga agttcaagct
    27901 gagcaggcta aaaaagcact cgaagcaaaa ggtgtaaaag tagaagtgac aacagttacg
    27961 acgacaaacg atgttcagca agctattact tcacttgcag gaaaagtcga tgctatctat
    28021 cttccaactg ataatacagt tgcctctaca gcttcaacaa tcggtgatat tttaaaagaa
    28081 gcaaaagttc cagctatggg aagtgacgcg gcagttattg acgcagcact atttacctac
    28141 ggtgtcgact accatgctat cggtgttcaa gctggtgaat tggctgtgaa gattttgaag
    28201 ggtgaaaagc ctgctgactt agcagttgaa aaaccaaata cagcggctat taccgtcaat
    28261 gaagaaatgg ctaaagctgt tggtattgat gcagcgacga taaaagcatt agaagagtaa
    28321 aatatttagg agaagaaacg tttatgtatt ataagctagt aaaaaaattg gcaactattt
    28381 cagtagcaag tatgggtttg ctaacacttg cagcttgttc ctcatcatca gaacaagctt
    28441 cctcagatgt ggtcaaagtt ggagttctcc aatatatgga gcatgaatca ttgacagctg
    28501 cccgtaaggg ttttgtggtg gaattagaag caaatggcta taaagagggt gaagaattgg
    28561 ttttggatta tcaaaatgcc caaggcgatc aagctaacct tcaaaccatt tcagaacaat
    28621 taatcgatgg aaatgatatt gtcttggcaa tcgctacgcc gtctgcccag agtttagcga
    28681 ctgtgtctac tgaaacgccg attgccttta ctgcggttac agatcccttg tcagctgact
    28741 tggtagaatc cattgaaaaa ccaggtggac ttttgacagg tacttctgat caagctccaa
    28801 ttgataaaca agttgaattg ctaggtcaag ctgttccaga tgctaagaca gttggtattt
    28861 tgtacactac tagcgaacgt aattcagaag tccaggtaga gcaagctaag gaattgctag
    28921 aaaaagcagg ttataaggta gtagtaaaag gaatcacatc ttcaaatgaa gtccaagatg
    28981 cgacaactag cttgatgaag gatgtagatg ccctctttat tccaacagac aatactgtcg
    29041 cttcaaccat gaccatgatc ggtgaattat ctgtggagca tagggttcca gtaatcggtg
    29101 gttcaacgga catggtggat gaaggaggtc ttttgactta cggtaccaac tatgaagctt
    29161 taggtcgtca aactgcaaaa atggctatta agattatcga aggagctaat gtgtcagaaa
    29221 cggcagtaga atacccagag accgtcagtc ttcacgttaa tgaagaaatg gctcagaaat
    29281 taggtattga tacttctaag cttgctgtat ctgaataaga ttggtaagat agaacctagt
    29341 atcgactacg aatttagaaa aaggagaaaa tcgtggatat tattttatca agtatctcgc
    29401 aaggcttgct ttggtcagtt atggcgattg gtgtttactt gacgtttcgt attttagata
    29461 ttgctgatat gacagctgag ggatcttatc cgcttggagc tgctgtttgt gcgacaggga
    29521 ttgtgaacgg agtgaatccc ttgctggcaa cctttatggc tatggtagct ggtatgggtg
    29581 caggcttgat ttctggttta cttcatacaa aactaaaaat tccagctctt ttgaccggta
    29641 ttgtaacctt gacaggtctc tactctatca acttgaaaat cttaggcaaa gccaatgtag
    29701 ctctacttaa gcaagaaact ctagtgaccc aattgcagga tttcggtttg acaaaaacaa
    29761 atgccgtttt ggtgattggt ctggtctttg tactagcagt tgtaggctta ttgacgctct
    29821 tgctcaatac acaaattggt ctagctattc gttcaacagg ggataatatt ccaatgagtg
    29881 aggccaatgg tatcaatgta gacaatatga aaatctacgg ttatatgttg tcgaatggtc
    29941 taattgccct ttgtggtgct ttgttgacac aaaacaatgg ttatgcggat ttgaactctg
    30001 gtacaggtac cattgttatc ggtttggcct ctgttattat tgcagaagta attttacgca
    30061 acttgcgctt gggttggaga ctcctatcgg ttgttctcgg tgcagttgtt taccgtttga
    30121 ttatcttagc gattttggaa attccaggta tggatgcaga ccttgttaaa ctcttctcag
    30181 ctatccttct ggcaaccgtt ctctatgtgc cagaattgca gaagaaattg aatattcgtc
    30241 gtccaaaatt gaatggaaat gcttaggagg aagtcatgtc tacaatttta tcaattcaaa
    30301 atattcataa aaccttcgaa gctggaacag ttaatgagaa ccatgtcctt cgtgggttga
    30361 acttagatgt aaaagagggg gattttatct ctattatcgg tggtaatggt gctggtaaat
    30421 caactttcat gaactcactt gcaggtgcct tgacggttga ttcgggcgat attttgcttg
    30481 aaggtaaatc cattaagcat gtctcagctg ccaaacgctc taaggacatt agtcgagtat
    30541 ttcaagatcc aaagatggga acagcttctc gattgaccat tgaagaaaac atggctattg
    30601 cctatcgtcg tggattgtca cgtggtcttg gctggggtgt gaaggacagt gaacgtgcta
    30661 tttttaagga agccttgaaa gagcttggtc tgggattaga aaaccgtatg aaggttgaca
    30721 cgcaatttct ttcaggtggt caacgtcagg ctctgacctt gatgatggct tcgctggtta
    30781 aaccaaaggt tctacttttg gacgaacata ctgctgccct tgatccaaaa accagcgata
    30841 tggttatgga attgactaag aagattgtcg agagccacca gttgacagct ctgatgatta
    30901 cgcataatat ggaaaacgcc attgaatatg gtaatcgttt ggtcatgttg caccgtggtc
    30961 agattgttgt cgatgtagaa ggagaagaga agaagaactt gactgttcaa aacttgatgg
    31021 accttttcta caaaaacagc ggtgaaaagc taactgacga tgagatgatt ttataaaaaa
    31081 taagactact gatttcatgc aagtcagtag tcttttttct tatatcgtca tttatactct
    31141 tcgaaaatca aaatcagacg ttgttgactt gatttgatga acttcagttc tatctgcatc
    31201 tgcgtcgcct agtctgtttt tgattttcat tgagtattag ttgactttta ttacttcgat
    31261 gtaaaagtca actaaatgac tatacttgta gaaattctag catttcttcc attggaattt
    31321 gatggatggc agcctgtccc aattgaggaa cgatgacaag tttgatggtc ttgccacgtg
    31381 cttttttatc gtgagttagg gcggcgtata attcatctac attccaaggc tggtgtgtag
    31441 tcggtaagcc gaatttttca cacatggcga taatcttttc tgtcatacca gctggcatca
    31501 gtcctttttt ctcagctgcg cgtgagattt gtaccatacc aatggcaaca gcttcaccgt
    31561 gcatgacctg gccgtagcca gcggtcgctt caatagcatg tccaatcgtg tgaccgaagt
    31621 tgaggtagag tctgacaccg ttgtctaatt catcttcgac aacaaccttg cgtttgactt
    31681 cacaggagtg gtagatgata tagtcagcat gctccagaat gctctcgaca gagccgtcca
    31741 attggtctag ggtttcccag agctccacat catctattaa gccatatttg acaacctcgc
    31801 ccattccttc aatcaattct cgtttgccga gtgtttttag ggtatcaggg tcaatgagaa
    31861 tgccgtctgg ctggcagaag gtgcccacca tatttttggc aaatggggtg ttcacgcccg
    31921 tttttccccc gatagaggaa tcaacctgag cagttaggct ggttgggatt tgtaggaaat
    31981 ggatacctcg catataggta gaagcgacaa aacctgccaa atcgccaacg actcccccgc
    32041 cgagagcaat gattccatca cttcgtgtca taccattttt tacaagaaat tcgtaggcct
    32101 gattgacggt atcaagattt tttgaggctt caccttctgg aaattcaaaa acaatggttt
    32161 caaaaccagc ttgttctaag cttgactgaa ctgtttcgcg gtagagtgag ccaacgtggt
    32221 catctgtaat aatggcgatt ttctgaggtt tccaaagttc tttgacccat gcacctgtct
    32281 gtgctaaact tcctctttgg ataaggatgt cgtagggagt gttgggaaga ttgacggtca
    32341 gtttcatcag atttcctctc tatttgatgt cgtatttctg gactaattgg tcccaaatca
    32401 agtcagtcgg catttcttgt ccagtccagg cttggaaggc ttcagctgct tggaaaagga
    32461 gcatgcccaa accgttaata gtagggttac cttgtgactg ggctagtttt aataaaggtg
    32521 tttcgaatgg ttgataaatc acatccgcaa ccaaaagatt ttcgggaaag cgaatggtag
    32581 aaggaacagg ttgagaatac ccatccatgc ctacactggt tccgttgact agtaagtcgg
    32641 agttggtgat atgttcctgt agtaattggc tatcttcaag cgctaggacc tgtatgggaa
    32701 ctccagttgc ttgagcaata ggtgtaagac tcgtctgtgt tctttccaag ctttgttgac
    32761 ggcagaaaat ggtaatttcc tttgcaccat ccaatgtagc ctgagctatg atagcagtag
    32821 aagcaccacc accacctaga atagtcagtc gcttgttgcg tacttggaat cctcttagtt
    32881 tttctaaact tttgaagaat ccaatgccat cggtattatg accgattagt aagccatcct
    32941 gatgaatgac ggtattgaca gcaccaatca agcgagcaga gtcagtcagg ctattgagat
    33001 aagggatgac agcctgctta tagggcatgg acaggttgat accaaacata tcatatcgtt
    33061 tgatattttc aagcgttaca gccaaatctt cctcgggaat ttcccaagcg acatagacac
    33121 catttacacc tgtttcctta aaagccagat tatgaataaa gggggaaatg gtgtgtttga
    33181 ttggctttgc aacaacggca gcaagacgag tgtagccatc aatgttcata gcttacctcc
    33241 aaattattta tcgtataaaa attaaggaaa gaattagaca gtttgatgaa tttcacaaat
    33301 cagagcttcc gttttttccc agcctaagca agggtcggta atggatttgc caaatacctc
    33361 agggctatct tgacggccgt cttctaagta ggattcaatc atgaaaccac ggacatattt
    33421 gcgaatagat tcgttccaag ctcggttgat gagggtttga cggacgatac ggatttgctc
    33481 caggtaattt ttaccagaat tgtcgtggtt ggtatcaatt acgataaatg gatttttaag
    33541 accaaattgt tcatactgtt tgatggtttt caagagaata tcatagtagt agtttggctc
    33601 ataaccgccc ttttcagtat tggcaccacg aaggatagca tgagctagag ggttgccatc
    33661 tgtatcaact tctgcatcac ggtaaataaa gttctgtttg ttttgtgcgg cgtagatacc
    33721 attgaacata acggtcaaat taccagaagt tgggtttttc ataccagttg gcatatcgac
    33781 acctgatgca acaaatctat gttcttggtc ttcaaccgaa cgtgctccga tagcatggta
    33841 ggacaccaag tcttccacaa gagggtagtt ggcagagtag agcatttcat cagcagttgt
    33901 gagtccggtc tcagtaatga cacggtaatg caaattacga acggctgcaa ttccgttaat
    33961 gaggtctggt aatttagagg tatctggctg atgaaccagt cctttatagc cttccccatt
    34021 ggtacgtggc ttagctgtgt aaacccgcat gaccataaag attttatctt tgacttcctc
    34081 ttgaagctta gagagacgac gtgcatattc taggacggcc tcttcattat ctgatgagca
    34141 agggccgata accaagagca gacggtcatc ttcgcctcgg ataattcttt tcagttcttc
    34201 atcgcgctga ttttttgcag ctagaaattc tccttctaac ttggaaagtt cctttacttt
    34261 atcgatgtct aaactggtgc tacgtttgtg aattcccata atatcttcct atctgttgta
    34321 aatttctcgg atgagttctt gtgttttttc ccagcctaag caagggtctg taatggattt
    34381 accataaata tctggattgt cttggcgacc gtcttcaata taggattcaa tcatgaatcc
    34441 gcgaacgtag ttacgaatgg attcgttcca gtcacggttg attaaggttt gacggacgat
    34501 acggatttgt tccaaataat ttttcccaga attgtcatgg ttggtatcga tgatgatgaa
    34561 tggatttttc aaaccaaatt ggtcatagag ctcaatggtt tctagaagat tgtcatagta
    34621 atagtttggt aggtatttac cattttcatt gactgcgcca cgaagaataa cgtgagcaag
    34681 tgggttgcca gatgtttcaa cttcagtatt gttgaagagg aaggattgct tcttctgagc
    34741 agcaaaaaga ccgttgaaca tgactttgag gttaccagat gttggatttt tcaagcctgt
    34801 cggaacatcg atgcctgacg ctacaaagcg gtgctgctga ttttcaaccg aacgagctcc
    34861 gatagcgatg taggagacca agtcatcaac caaaggcagg ttttcagggt agagcatctc
    34921 gtcagcagta gttagacctg tttccgtaat gacacgatag tggaggttac gaactgcttt
    34981 gattccgttg atgaggcttg gtgcagcatc tgtatctggt tggtgcatga gtcctttata
    35041 accatcacca ttggtacgtg gcttggcagt atagacgcgc atgaccatga aaattttatc
    35101 tttgatctct tcttggagct tggccaagcg gtgagcataa tctaagacag cctcttcatt
    35161 gtcagaagaa catggtccaa ttactaggag aagacgatca tcttctcctt ttaagatggc
    35221 ttcaagttca cggtcacgag cttgtttttt agcaagtgtt tctggagaaa gttttgaatg
    35281 ttctcgtact tgattgatgt caattttttc gctgattggt gtaaacaata gatctccctc
    35341 tttcatttat agattcctca attataccat tatttttaag aatttaaaat caatatccat
    35401 gattttatgt aaaatttaca gtaaattctg aaaattcttt ctttaatttc agtagatttt
    35461 gagtatcgct tttatttaga tataattttc caaaaaaatc tgaaaatatt caataaatat
    35521 gatagaatag gtctaacact atttcgaggt atgagttatg aaattaagaa caaatataga
    35581 aaaattacaa ggaaaaattc gagttccggg tgataaatca atcagtcatc gctctattat
    35641 ttttggttcc cttgcaaaag gtgttacccg tgtccacgat attttacgtg gggaagatgt
    35701 cttgtcaacc atgcaggtct ttcgtgattt gggtgtaaaa attgaagaca atggtgatat
    35761 tgttgaagta cacggtgttg gctttgatgg tcttcaagcc cccaaaaatg atttggatat
    35821 gggaaattct gggacctcga ttcgtttgat ttcaggtgtt ttagcaggtc aagattttga
    35881 ggcgactatg tttggtgatg attccttgtc caaacgtcct atggaccgag tgactattcc
    35941 actcagtcaa atgggagtag agatttctgg acaaactgaa cgtgacctac ctccgttgac
    36001 catcaaaggg aataagaatt taaaaccaat tcgctaccaa ttgcctgtag cctctgccca
    36061 agtaaaatca gctctgattt ttgctgcctt gcaggctgag ggagagtcgg tcatcgtaga
    36121 aaaagagttg actcgaaacc atacagaaga tatgattgtt cagtttggtg gacagttgga
    36181 agtcaatggc aaggaaatcc gcatccaagg tggtcaggag tttattgccc aagagattac
    36241 agttccagga gatatttcaa gtgctgcttt ttggttggtt gctggcttaa tcgtaccagg
    36301 ttcaaaaatt gtccttgaaa atgtgggaat caatgaaact cggactggta ttctagatgt
    36361 cattaaagct atgggcggaa aaatgactct ttctaacata gatgaacttg caaaatctgc
    36421 taccattaca gttgacatgt cggaattgaa ggctacggag attgcaggcg aattgattcc
    36481 tcgtttgatt gatgagttgc caattattac cttactagcg actcaagcac atggaacaac
    36541 gattattcgt gatgctgagg agttgaaagt caaagaaacg gatcgtattc aggttgttgc
    36601 agatgctcta aatagtatgg gagcaactat tgaaccaaca gaagatggta tgattattca
    36661 cggaccaact gctttacatg gtgctgaaat caatacattt ggtgaccacc gtatcggtat
    36721 gatgactgcc attgccgcct tgttggccaa agatggagaa gttgtattgg aaagagcaga
    36781 agctatcaat actagctatc ctgccttctt tgaacactta aatagtttga tggattagga
    36841 gaacgtatgc caatcgtttt acttggattt atgggagttg gaaaaacaac aacagcacat
    36901 ttgttaaatc tccccgttta cgatatggac catatcattg aagaacggat tggtatgcct
    36961 attgctgcct atttcagtct tgaaggagag gcttcgttta gacaactgga gacagaggta
    37021 ctgaaagaat tgctggactt acctagtaat tgtatcgtgt caactggtgg tggggtgatt
    37081 aaatctgaag taaacaggga attgctgttg gcaaataggg aggacaatgt acttcttact
    37141 gcctcctttg aagtttccta tcagagaatt cgtaaggata gacaatcgca aagacctctc
    37201 tttttacagt gtagtaagga agagtttgaa gccctctatc gtgaacgaat ggcactttat
    37261 caaggtttag ctgatactgt aatcgataca gataaactga atccagaaca agtagcaagg
    37321 aaaattttat gcaagtagcc tatctaggac ctcgtggttc ttttacacat caagtggccc
    37381 agcaggcctt tccgagtgcg gatttgcagg cttttgagac gattacagag gttatcaagg
    37441 cctacgaaac gggagaagtt acctattccg ttattccagt agagaactcg attgaaggta
    37501 gtgttcatga aacgattgac tatctctttc atcaggcgga gattcatgcg gttgcagaga
    37561 ttgttcaacc gattgcccaa caactgttag ccacgagtgc cgataagact gtagaggtta
    37621 tctattctca tccgcaggca attgctcagg ggaagaaata tattcaggaa cattttcctc
    37681 aggctcgtat tgaaataaca gctagtacag cctatgctgc tcgctttgtg gcagaacacc
    37741 ctgaacaacc attcgcggca attgctccgc aagcggcagc agttgaatat ggactggagg
    37801 taattgcgca agacatccag gaaatcagcg agaattttac acgtttttgg attttggggg
    37861 ataaggctcc gtctattgcc ctggaacaag tagctaaaaa agtcagtctt gcactgacat
    37921 tgccagataa cctgccagga gccttgtata aggccatgtc tgttttctca tggagaggaa
    37981 tcagcttaac aaaaatagaa agccggcccc tgaaaacggc tttgggtgag tattttttca
    38041 tactggatct tgacaatcag tctgaggaat tattagccta ttctcaaaaa gaattgacta
    38101 gtttagatat aacctataaa gtactaggga attacctggt ctatatgacc agctaggatg
    38161 aagacagatg aataaaaaca ataatatcct aacccatcat gaacaattga ggttagatta
    38221 tttgcaaaaa aatatccact acttaaatga caaagaaagt cgtgagttgg attatctatt
    38281 atataaaaaa gagctgagag aacggcatgg aagtccaagg caatctctac gtagcttcac
    38341 accaacggct tacgaagatg acttcgagga agattatttt gaagaggatg atgagcaaga
    38401 ggaactgtta cctcgctatc ctgaacgtaa atctagaaaa acaagacaag ccaaacctca
    38461 acggtttcag tctcctcgta tcgagcccaa agcgaaagta cctaaaccta aaaaagctcg
    38521 taaaaagggt ggtttcaagc gctttattgg ttttgtattg cttgctatag cgctagtgat
    38581 tgtagcgatg ggctatagtt ttttcaaagg tatgaatagt gtgagtgaga agccagtagc
    38641 tgaggtcttc aacggcgtag atacatcgaa tgggacaaat attttaattt tgggaacaga
    38701 cggtcgtgtt ggcgaaagct caggtgaaac tcgaacggat accatcatgg ttctaaatgt
    38761 gaataatacg gataacaaag tcaaacttgt tagctttatg cgggatacct tggtggatat
    38821 tgagggatat gaatataaac tcaatactgc ctatactttg ggagaacagg ataatcagca
    38881 aggggctgaa gaagttcgga aggcgttgaa gaataatttt gacattgata tcaagtacta
    38941 tgcgatggtg gattttgcaa cctttgcgac aatgattgat acacttttcc ctgagggcgt
    39001 aacgattaat gcacggtttt caactattaa tggtgaagtc gttagctcag tagaagtgcc
    39061 gaatgattta cagctggaag aaaatgctcc tgcttatcaa acgattcagg tcggtgtaca
    39121 gcagatggat ggtaagacct tgcttaacta cgcgcgctat cgttcggatg atgaaggtga
    39181 ttttggtcgt acgagacgtc aacaggaagt gttatctgct gttatgacac aggctaaaaa
    39241 tccaatgaaa ctattctctg gcttcgaagc tataggtaag gttgtggcca tgactccgac
    39301 aacagtgcca caaagcttta tgtggctaca gggaccaggc cttctattgg atgcggcaaa
    39361 tggtatcgaa cgtgttacaa tcccagaaaa tggagactgg attgacgact atgatatgta
    39421 tggtggcatg gctcttcgtg ttgattttga aaaataccag cagcgcttgg ctgagttagg
    39481 cctgcgttaa gaaatatggt atactagagg tgacttcggt cactttttta gtggtccaaa
    39541 aatagaaagt agaattatgt taaagaaaaa cgatattgtc gaagtagaag ttgtagattt
    39601 gacacatgag gggcagggtg tggcaaaaat tgatggtttt gtcttctttg tagacaatgc
    39661 tcttccaggc gagaaaattt ccatgcgcat cttaaaactt aagaaaaata ttggttttgg
    39721 taaggtggag caatggcaga gcttctcacc aaaccgtaat caggacttgg atttgaccta
    39781 tctaagaaca ggtattgctg acttggggca tctcacttat ccagcccaac tagcttttaa
    39841 gaaaaaacaa gttgagaata gtctacgtaa gattgcaggc atttcagaaa tagcagtagc
    39901 agatacgttg ggaatggaca atcctcttgc ttatcgtaat aaggcagctg ttccagttcg
    39961 tcgtgtgaat gggcagttag aaacaggatt cttccgaaaa aattcacatg atttagttcc
    40021 gattgaggat ttttacattc aacataagga aattgatgcc ttggttctag cgacacgtga
    40081 tctgttgaga aaattagact tgaagcctta cgatgagaaa gaacagacag gacttgtgag
    40141 aaatattgtt gttcgtcgtg gtcattattc aggtcagttg atgctagttt tagttacgac
    40201 tcgtccgaag attttccgtg ttgaaaccct tattgagcgt ctgacaagcc aatttccaaa
    40261 tcttgtttct atcgttcaga atactaatga ccagaatacc aatgctatat tcggtcaaga
    40321 attccgtctt ttgcatggca gtgaaactat tgcagatacc atgttgggca atgaatttga
    40381 aatttctgca ccgtctttct atcaagtcaa cacagaaatg gcagaaaaac tctatcagac
    40441 cgccattgat tttgcagaat tgtcggcaga tgatgtcgtg attgatgcct attcaggtat
    40501 tgggacaatt ggtctctcct ttgcaaaaca agttaagcat gtttacggtg tagaagtcgt
    40561 tgaaaaggct gtccttgata gtcagaagaa tgctgctcga aatgggattg ataatgttac
    40621 ctatgtctgt gatagtgcag aatccgctat gcaaaaatgg gtggtagatg gtattaaacc
    40681 aacagtgatc tttgtcgatc ctccgagaaa aggtttgaca gaaagtttta tcaaagcaag
    40741 tacttctgta aaaccagaga aaatcgtcta tatctcctgt aatgtggcaa ccatggcgcg
    40801 tgatattaaa ctttatgagg aattggagta caaattgacg aaggtacagc cgactgattt
    40861 atttccaaat acgcatcacg tggagtgcgt agtgttgcta caacgaagca acgggtaaaa
    40921 atcctttatt ttaagcactt tttcaagcat tttgtcttta ttgaaaagag tgattttgac
    40981 ataaaaaagg ttcaaaaaaa gtacattgat gtgaacgtct gtttggatgt cgactgcgta
    41041 gacaaaaaat agatacgtca taaaatataa tagttcagcg agaacatttt aataagtgtt
    41101 ctcgtttttt tattttaagg aggaattcat gaaaaagaag gaacaatcat cacgccaaat
    41161 cgtgatgtgt catcttgtgg ctattttagg agttgatatt gagaaagcaa ctcatctggt
    41221 tgatgaattg gaacaagtag gtttaatccg atttgatgaa ttcggaaatg ttggactttt
    41281 agtcttggag ggataatcat gaaacgtatt accgctaatc aatatcagac atcagagcgt
    41341 tattataaac tcccaaaagt cttgtttgag agtgaacgtt ataaaggata tgaagctgga
    41401 agttaaggta gcctacgcgg ttttaaaaga taggttggag ctgtctttga gtaaaggttg
    41461 gattgatgag gatggggcta tttatttgat ttattccaat tcaaatctga tggcactttt
    41521 aggctgttca aagtcaaaac tactctctat caagaaaacc ttacgcgaat atggcttaat
    41581 tgatgaagtc caacagtcct ctagtgaaag aggtcgaatg gcaaataaaa tttacttggg
    41641 ggaattagaa catgaaacta ccccagtctt acatacagac ggggctagtg ttaaaaaaac
    41701 actagggggg tctcaaagaa agacggggcc ggtcttaaat tcagccccta gtgagactga
    41761 aagaagtgag actaaatata gtgaaactaa agggagtgat ttccttattg aggacgagga
    41821 ggagaggcag ctagtagatg agaaacaaga agagaacttt acttcaaaag tcgatggcgt
    41881 gaccaagtac gatcgagact atattttggg tttggttcat gaccagttaa gacagacggg
    41941 tctatctcag tcagctagtg actatgccat gatttatttt agtgaccgtt atcagtatgc
    42001 tttggaacat atgcgatttg ctcggtcagc ggaagtaata gctgaatacg tatttaatgg
    42061 tgtgctgtca gagtggacta agcaactgag acgacaagaa gtaaagggag gtgattaagt
    42121 tgatttggtg gatactaggt ggaatctatc tgatttctat cgtcattttg attgttgaaa
    42181 tcatccgtgc acctgaaatg gatgatcata tataagcaag agttttacaa gtgtaaggct
    42241 cttttttagt tggaaagaga ggaaaaatga aatttttaga tttatttgct gggataggcg
    42301 gttttaggct agggatggaa tcacagggtc ataaatgcct gggcttttgt gaaattgata
    42361 aattcgccag aacatcttat aaagccatgt ttaacacaga aggggaaata gaataccatg
    42421 acattaaaga ggtcacagac catgacttta gacaatttag agggcaagtg gacatcatct
    42481 gcgggggatt tccttgccaa gcattttcac tcgcaggcag acgattggga tttgaagata
    42541 ctcgagggac tctctttttt gagattgctc gagcggccaa acaaatccaa ccacgttttc
    42601 tatttttgga aaacgtcaaa ggcctactca atcacgacga gggacgtacg tttgccacaa
    42661 tcctctccac gatggatgaa ttggggtatg atgtcgaatg gcaggtgctt aacagtaagg
    42721 acttccaagt cccgcaaaac agagagcggg tctttattat cggacattct agaagatacc
    42781 gttccagatt catatttcct ctcagaagag aaaacagccc agctcatctt gaaaggccag
    42841 gaaatatcaa tccctctaaa cgtggttcga atggtgaagt ctatctgacg aatggacttg
    42901 ctcctacact aacaagaggt aaaggagagg gagctaaaat cgccattcca gtcttaacac
    42961 cagatagact agaaaaacgg caacatggtc gtcgacttaa ggacaatcaa gaccctatgt
    43021 ttactttgac cagtcaagac aaacacgggg ttgttgtcgc aggaaatctg ccgactagct
    43081 ttgaccagac cggtagagta tttgacattt ctggcttgtc accgaccttg accaccatgc
    43141 aaggtggaga caaggtgcca aagattttgc tgagggagga gctgccattt ctgaaaatca
    43201 aggaagccac aaaaacaggg tatgcaaagg cgactcttgg agactctgtc aatctggctt
    43261 atccagactc aaccaaacgt aggggacgag tgggaaaggg aatatccaat actctgacga
    43321 cttcagacaa tatgggagtg gtgattgctg ctctggaata tcgacaggat aagtggtatg
    43381 aagtcacagg cattgtctta gaggggaaac tttatcgctt gagaataaga cgactgacac
    43441 caagagagtg tttcagactt caaggctttc ctgactgggc ttatgaaaga gcagagagtg
    43501 tttctagtaa gagccaacta tacaaacagg ccggcaatag cgtgactgtc acagttattg
    43561 aagccattgc cagagaattt agaagaacga agaacggaag aggaagaaaa acatgaacct
    43621 actacataag aaaagtattc tagagtgtac tgaattagaa gaacatattc accaagttga
    43681 aaccaatcag ttattacaaa agatactgtc gctccccaat tttgattgtg actttgaggt
    43741 gacttttgaa gatgattacc acaatgagat gaatgatccc ctattctacg aatccaatct
    43801 tcatcagatt tcggatttta tggaaactag ggatattaaa aatggtgtag atacactatt
    43861 gacgaaagac aatcacctgg cctttcgtgc ctttggtgaa aattattctg ctagaggaaa
    43921 ggatggcatt ttaacgactt tagtgacggt caagtgcttt ggtgaaggac ggatgcccat
    43981 tgatatgagt cgctatttct caactcctga accaacagtt gaaaatagcc taaccttata
    44041 aggaggagcc tatgcttgag gtatatctag gaaataatgc caatacaaat caagatttac
    44101 taaccatttt gacgacctat ggtgtgactt atcgttgtat aaatgcgtgt gaggtaaatc
    44161 gtgaaatctt actgtcactc tttgctaaaa ccacggattg ttttgagttg ctgtcgccac
    44221 gatttcttcg ctttaagcgt caatatacga taagtttgaa tgaaatgatt cagctcattc
    44281 tccaaaagcc agaccaaaac cttcgtctgc ctctcattgt ctgtcagaat catatctatc
    44341 cagctattgg actagacgaa gtacgaactt ttcttcccag acaggtaaaa gaagagctgt
    44401 ttcaggccag tctgatgaaa caagtgacag ggtaggtgtg aagatgaatg aacgattttg
    44461 ggacaattta gaaatcattc tggcagaaaa agacctcact tgggcagaac tagctcgcaa
    44521 agtattcaac ggtcaatatg tttatccaag tgagtttaat cgcctctatc aaaaattacg
    44581 gcattacaaa tctaatcgtc tgatgccaca aacaagatgg gttgagcgaa tcgttctagt
    44641 cttagatatt gattatgaag atttatttaa gaggtgacaa tgataaggat agtgttgttt
    44701 tatctagcca tacagcttaa tggacttctg gtgagtttat acctgaaaga gtatctgaaa
    44761 atagagggta tagtcttgct acaattggtc ctattaagtg tgacttgctt agagattgcc
    44821 cgtcataaaa ctgttcaagc aaaaaaaata accttaagca atcgtctaag atgcttgctt
    44881 cttggttttg tgtgtatggt tgcttttgca gtcttcatca gcttcctatc tccagttcag
    44941 actaggaatc aagcggtatt ggtacaagta ggaaaacaag ttcctcctat tatcttt
//
LOCUS       DATUCG010000004        41772 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.004, whole genome shotgun sequence.
ACCESSION   DATUCG010000004 DATUCG010000000
VERSION     DATUCG010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 41772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41772
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.004"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            458..1951
                     /gene="sly"
                     /locus_tag="VCA20_000168"
     CDS             458..1951
                     /gene="sly"
                     /locus_tag="VCA20_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_095889394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin suilysin"
                     /protein_id="HEP1834042.1"
                     /translation="MRKSSHLILSSIVSLALVGVTPLSVLADSKQDINQYFQSLTYEP
                     QEILTNEGEYIDNPPATTGMLENGRFVVLRREKKNITNNSADIAVIDAKAANIYPGAL
                     LRADQNLLDNNPTLISIARGDLTLSLNLPGLANGDSHTVVNSPTRSTVRTGVNNLLSK
                     WNNTYAGEYGNTQAELQYDETMAYSMSQLKTKFGTSFEKIAVPLDINFDAVNSGEKQV
                     QIVNFKQIYYTVSVDEPESPSKLFAEGTTVEDLQRNGITDEVPPVYVSSVSYGRSMFI
                     KLETSSRSTQVQAAFKAAIKGVDISGNAEYQDILKNTSFSAYIFGGDAGSAATVVSGN
                     IETLKKIIEEGARYGKLNPGVPISYSTNFVKDNRPAQILSNSEYIETTSTVHNSSALT
                     LDHSGAYVAKYNITWEEVSYNEAGEEVWEPKAWDKNGVNLTSHWSETIQIPGNARNLH
                     VNIQECTGLAWEWWRTVYDKDLPLVGQRKITIWGTTLYPQYADEVIE"
     gene            2269..2991
                     /locus_tag="VCA20_000169"
     CDS             2269..2991
                     /locus_tag="VCA20_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEP1834043.1"
                     /translation="MAIISKEILKEQIKDGIIVSCQALPGEPLYREEGGIMPLLVKAA
                     EEAGAVGIRANSVRDIKEIKEVTNLPIIGIIKRDYPPQEPFITATMKEVDELAELDIA
                     VIALDCTKRERFDGLDIVDFIKQIKEKYPHQLFMADISTYEEGVTAYEAGIDFVGTTL
                     SGYTSYSRQEDGPDMELVDRLCRAGIDVIAEGKIHYPDQARTIHDLGVAGIVVGGAIT
                     RPKEIAERFIQAIEKFYGGKND"
     gene            2984..4117
                     /gene="ugpC"
                     /locus_tag="VCA20_000170"
     CDS             2984..4117
                     /gene="ugpC"
                     /locus_tag="VCA20_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEP1834044.1"
                     /translation="MTELKLKHLFKKYPNSTYYSVEDFDLEIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDITEGELYIGETLMNDIAPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYSKSEIENRVNEAAEILGLKEFLNRKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRTEIAKIHQRIGSTTIYVTHDQTEAMTLADRIVIMSATKNEEGT
                     GTIGKIEQVGSPQELYNYPVNTFVASFIGSPAMNFIKLFVGESNIYNDYGFDLELPEG
                     VKKVLSENGYIGKEIIFGIRPEDISADLISSTTFPNATIETEVVVSELLGSDSILYCN
                     TNNIEFSARVAAHDHRQAGEKIRLAFNISKGHFFDIQTEKRIG"
     gene            4126..4575
                     /locus_tag="VCA20_000171"
     CDS             4126..4575
                     /locus_tag="VCA20_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEP1834045.1"
                     /translation="MIITNILNLERYNSINPQFWKLQKFIEENDLVNLPEGKIEIDGE
                     EIFGNCFSYIADGKPGEFFETHRKYIDIHLVVNNIEAMATSSTNCTSVTSPYCEEKDI
                     ELFEGEIEQIIKLNAGTCLITFPEDLHQPKIGINEFPVKKIVFKVKI"
     gene            4598..5950
                     /locus_tag="VCA20_000172"
     CDS             4598..5950
                     /locus_tag="VCA20_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEP1834046.1"
                     /translation="MFKIKKIIKHAGLILTAIGLAACSSGSSATNIAPEKNGELEGEI
                     VMWHSFTQGPRLESIQKTADEFMEEHPKVKIKIETFSWGDFYTKWTTGIANGNVPDIS
                     TALPGHVMEMINSDAIIPLNDSIEAIGKDRFNETALNEAKIGTDYYSIPLYSHAQVMW
                     VRTDLLKEKNIEVPKTWDELYEASKKLTEGDEYGMSVPFGTNDLMGTRFLNFYVRSGG
                     GSLITKDLKVDLTSKLAQDGIKYWVKMYNEVSPKDSLNFNVLQQATLFYQGKTAFDFN
                     SGFHIGGVEANSPQLIDSIDAYPIPKINESDETNGLETSNIPMVVWKNSKHPEIAKAF
                     LESLYEDENYLEFLDSTPVGMLPAISGITDLPSYQENETRKKFKHAEEVILEAVKTGT
                     AIGYENKPSVQAGLLTNQHVIEQMFQEIITNGKDPMEAAKDAEKQLNDLFDSVAVDVE
                     "
     gene            5985..6869
                     /locus_tag="VCA20_000173"
     CDS             5985..6869
                     /locus_tag="VCA20_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEP1834047.1"
                     /translation="MVKDRNLTRWIFVLPAMIIVGLLFIYPFFSSIFYSFTNKHLIMP
                     NYKFVGFDNYIKVLSDPNFFNAFFNSLKWTVFSLTGQVLVGFCLALALHRIRRFKKLY
                     RTLLIIPWAFPTIVIAFSWQWILNGVYGYLPNMIVKLGLMNVAPAFLTDRTWAFLCLV
                     FINVWFGAPMIMVNILSALQTVPQEQFEAAKIDGASTLQVFRYITFPHIKVVVGLLVV
                     LRTVWIFNNFDIIYLITGGGPSNATMTLPIFAYNLGWGTKLLGRASAVTVLLFIFLLA
                     ICFIYFGIISKWEKEGGK"
     gene            6869..7702
                     /locus_tag="VCA20_000174"
     CDS             6869..7702
                     /locus_tag="VCA20_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004635570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEP1834048.1"
                     /translation="MNAKKGIILDVISHLFLALATIVSIFPLVWIVISSVKGKGELTQ
                     FPTRFWPEQLTLDYFTHVINDLNFIVNIRNSLVLALSATVVATIISSMAAYGIVRFFP
                     KLGAIMSRLLVTTYIFPPILLAIPYSIAIAKAGLTNSLFGLMLIYLSFSVPYAVWLLV
                     GFFQTVPIGIEEAARIDGANKFVTFYKVVLPIVAPGIVATAIYTFINAWNEFLYSLIM
                     INDTSKMTVAVALRSLNGSEILDWGDMMAASVIVVLPSIVFFSIIQNKIASGLSEGSV
                     K"
     gene            7714..9810
                     /locus_tag="VCA20_000175"
     CDS             7714..9810
                     /locus_tag="VCA20_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HEP1834049.1"
                     /translation="MKNIKLLKKFSICLLATTMLTTLHPIFINAEDAIGTEIVNGSEY
                     HSGNFTLENSRIDITNEAFDKLNTESQTIVLKFQSDNPNSLQAFFGVSNSNSGFRNNY
                     FSIFMRDTGEVGFEVRDKNKGINYLFSRAASIWGIHKGQAVENTIVFTSDNESKTYSL
                     FVNGTKVLSETVENFLPITSIEGVDNVSVGGVNREGKDAYTMRGQVNQLSIYNRVLSI
                     DEINSKFGELPYQFIFQSGDATNANYFRIPTLYTLSNGRVLASIDARYGGTHDSRSKI
                     NIATSYSDDNGNNWSIPSLPLKFDDYAEQLIEWPRSGNLKNVQINGSASFIDSALVED
                     KTTGNVVMIADAMPAGIGNNNADRNDSGFKEIDGKYYLKLKKDGETTFNYSVREDGVI
                     YDDIANLPTQYRLNDKYEVLEGDVPLNVEQYSYSFDASGLQEFHNGNLVPMNVFYQDS
                     LFKVARTNYLGVTISKDKGETWSNFKLLPPMLGLNHNATYLSPGQGLSLMNSNRIIFA
                     TYTRGELTYLISDDGGVSWNKSSAGVPFQNATAEAQMVELKEGVIRTYFRTTTGKIAY
                     MTSYDSGETWSDVSYLDHISQTNYGTQVTAIKYSKKIEGKDAIILSTPNTNSGRRGGQ
                     LWVGLINPEDDTVNWKYSYNVDIPTFGYSYSALTELPNGHIGLLFEKYDSWSRNELHL
                     SNVVQYIDLETSDIIK"
     gene            10188..11291
                     /locus_tag="VCA20_000176"
     CDS             10188..11291
                     /locus_tag="VCA20_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEP1834050.1"
                     /translation="MIRYGVVGTGYFGAELARFLILNEGAVITKVYDPENAGPIAEEL
                     GALETQSLDELVSSDDVDCVIVATPNYLHKEPVIKAAQNGKHVFCEKPIALSYEDCKE
                     MVETCKQYGVTFMAGHIMNFFNGVQHAKKLINEGAIGDILYVHTARNGWEEMQETISW
                     KKIREKSGGHLYHHIHELDCVQFIMGGVPETVTMIGGNVAHVGENFGDEDDMIIVNME
                     FPGKKFAVLEWGSAYHWGEHYVLIQGTKGAIKLDMFNTKGTLKVNGNETNFLVHETQE
                     EDDDRTRIYYSTEMDGAIAYGKPGKRTPMWLSSIIKKEMKFLHEIMEGGPVSDEYKKL
                     LTGEAALEAIASADACTVSLNEDRKVKLTEIMK"
     gene            11416..12333
                     /locus_tag="VCA20_000177"
     CDS             11416..12333
                     /locus_tag="VCA20_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEP1834051.1"
                     /translation="MTLEKYHGIIPAFYACYDEAGEISSERVKALVQYFIDKGVQGLY
                     VNGSSGECIYQSVADRKQILEAVMEVAKGKLTIINHVACNNLKDSVELARHSEELGVD
                     AIAAIPPIYFRLPEYSIATYWNGISAAAPNTDFIIYNIPQLAGVSLTPSLYKEMLKNP
                     RVVGVKNSSMPVQDIDTFVSLGGEDYVVFNGPDEQFLGGRLMGAKGGIGGTYGAMPEL
                     FLRLNQLIADKDLETARQLQATINTIIGKLVSGHGHMYAVIKEVIRINDGLDIGSVRE
                     PLTALTEADLIIAQEATQLIRDARERFGA"
     gene            12343..13233
                     /locus_tag="VCA20_000178"
     CDS             12343..13233
                     /locus_tag="VCA20_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018367969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEP1834052.1"
                     /translation="MKTYLAIDIGGTQIKYGRLDEAGQILESYKIDTEAYKGGPHILA
                     TVKKLVEDFHAQVALSGVAISSAGMVDPDKGEIFYSGPQIPNYAGIQFKSEIETTFGL
                     PCEIENDVNCAGLAEGISGAGQGSQISICLTIGTGIGGCLLVDGQIFHGFSNSACEVG
                     YLHLSDGAFQDLASTTALVQEVARLHDQNPEEWNGYKIFQEAKAGDAHCIVAIERMVN
                     YLGQGIANICYVANPEVVILGGGIMAQKDYLYEKIQAALKENLVSSLADKTKLAFAEH
                     ENAAGMLGAFYHFQQKQGLR"
     gene            13328..14227
                     /locus_tag="VCA20_000179"
     CDS             13328..14227
                     /locus_tag="VCA20_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEP1834053.1"
                     /translation="MIDNMTLQDMLTYRGRQEVPADFDAFWQNEKNQLTGLTDHVLLE
                     KECGLPHVACYELTFTGTNGSKVFAKCLFPKSEQAVPVLFYFHGYQGQGPDWSENLKF
                     LAAGYGVVAMDVRGQAGRSQDLGQFEGITVKGQVIRGMLQGPEHLFYKDVYQLIEIIA
                     SLDFVDQKRLVSYGASQGGALALVAAALSPYISKTVAIYPFLSDFKRVLELGNTSEAY
                     DELFRYFKFSDPFHETEEAVLQTLSYIDVKNLAHMITCPVAMVTGLEDSVCPPSTQFA
                     IYNRLEVEKEMKILPEYATMPCM"
     gene            complement(14296..15117)
                     /locus_tag="VCA20_000180"
     CDS             complement(14296..15117)
                     /locus_tag="VCA20_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEP1834054.1"
                     /translation="MLETPKNMTAIIEAHLEQMTRLEKHIASYFTQLDPASADFSLEN
                     TTQTLHISPSALTRFAKKCGFSGYREFVFAFQNNRQYLDKHFEKLQRSLTKKVLVDYE
                     EILTATNKLVDEEKLEEIAKLIDSSKRVYFYGIGSSGLVAMEIKSRFMRLGVVCDAIT
                     DKNNLIWTTNILDQTCLVIGLSLSGQTEEVMEHLQLAAQKGIPTALLTTKSFPHPTFD
                     QIIPVASVRHLNYGNRISPQIPLLIMLDIIYAYFLAIDKERKENIFKHTIKDTTI"
     gene            15215..15712
                     /gene="ybeY"
                     /locus_tag="VCA20_000181"
     CDS             15215..15712
                     /gene="ybeY"
                     /locus_tag="VCA20_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEP1834055.1"
                     /translation="MYIEMIDETGQVCAQMQEQITELLQFAAEKIGKQNKEMAVTFVD
                     NKRVHEVNLEYRGIDRPTDVVSLEYKPENEIFFDEEDLQDNPELAEMMEDFDVYIGEL
                     YISIEKAREQAEDYGHSYEREMGFLAVHGFLHINGYDHYTPEEEAEMFGLQEEILTAY
                     GLTRE"
     gene            15693..16094
                     /locus_tag="VCA20_000182"
     CDS             15693..16094
                     /locus_tag="VCA20_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEP1834056.1"
                     /translation="MDLQENNSKNRWKNRELIASLEFAVTGLLTAFKEERNMKKHLAS
                     AVLVVLAGLIFQVSVTEWLFLLLSISLVIAFEIVNSAIENVVDLASDYHFSMLAKNAK
                     DMAAGAVLFVSGFALLTGLIIFVPKIWDFIF"
     gene            16111..17010
                     /gene="era"
                     /locus_tag="VCA20_000183"
     CDS             16111..17010
                     /gene="era"
                     /locus_tag="VCA20_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEP1834057.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNYVMGQKIAIMSDKAQTTRNKI
                     MGIYTTEEEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVDTVFFMVPADEKRGKGD
                     DMIMERLKQAKVPVILVVNKIDKVHPDQLLEQIDDFRQQMDFKEIVPISATQGNNVNR
                     LMEILKENLDEGFQYFPADQITDHPERFLVSEMIREKVLHLTREEIPHSVAVVIESMK
                     RDEFTDKVHIRATIMTERDSQKGIIIGKQGAMLKKIGSMARRDIELMLGDKVFLETWV
                     KVKKNWRDKKLDLADFGYNEKEY"
     gene            17215..17652
                     /locus_tag="VCA20_000184"
     CDS             17215..17652
                     /locus_tag="VCA20_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEP1834058.1"
                     /translation="MTIIAVNEKTLSIWATFASQVWQTSEQVLIEKFSNNEFPFEFLY
                     YSQSEEPIAWISLSLRQDYVEGCQTSPVAYLEGIFISPNYRSQGIAEELLHFAECWAR
                     SRGISQLGSDAELDNLLSQNFHIKHGFREVSRTVHYVRNLNIE"
     gene            18046..18873
                     /gene="mutM"
                     /locus_tag="VCA20_000185"
     CDS             18046..18873
                     /gene="mutM"
                     /locus_tag="VCA20_000185"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEP1834059.1"
                     /translation="MPELPEVETVRRGLNRLVKGKVIEKVKVTYAPMIKTGVDAFCQD
                     LLGQEILDVARRGKYLLIYLTDHVLISHLRMEGKYNFFPDQVPANKHFHAFFTFSDGS
                     TLVYQDVRKFGTMELLGKADVDAYFISRKIGPEPTEEDFDLEEFAKKLAKSKKPIKSH
                     LLDQSLVAGLGNIYVDEVLFRAQVHPAQASNQLSAEQVADLRQATIEVLQLGIEKGGS
                     TIRTYKNALGMDGTMQDYLQVYGKTGQACPRCQIEIVKIQLGGRGTHFCPTCQVKHG"
     gene            18866..19468
                     /locus_tag="VCA20_000186"
     CDS             18866..19468
                     /locus_tag="VCA20_000186"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEP1834060.1"
                     /translation="MAKIIGLTGGIASGKSMVTAFLREQGYPVIDADAVVHELQAKGG
                     KLYQVLLAEFGEAILSADGSLDRAKLGQAVFEDSKLRARLSDLQDQIIRQELLDRRDA
                     LKQTEDVIFMDIPLLYEADYSGEVNEVWLVYVDRAQQLERLMKRNGLAVQDAENRLAA
                     QLSLEEKWSQAQVVIDNSGAVEATLAQVAQLLEELKDGRR"
     gene            19455..20660
                     /locus_tag="VCA20_000187"
     CDS             19455..20660
                     /locus_tag="VCA20_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEP1834061.1"
                     /translation="MDDGSSYWKQNLKVAWLGNFLTGTSFTLVMPFISVFVEELGVGP
                     GQVEYYAGLAVSVNALAAALMAPIWGSLADRYGRKPMMVRAAFAMIFTMGGMAFVPNV
                     FWLLALRVLNGVFTGYIPNATALIASQVPKDKTGYALGTLSTGAVAGNLIGPTLGGIL
                     AEMFGVHMVFLLVGLLYAIVVLLTVFYIREDFVPIKKGEEMSVKEVFEQVKDRQMLVG
                     LFVTSMIIIAAAQAVVPILTLYVRHLGQTDNLLFVAGFIISLPGMASLVTSGYLGKFG
                     DRIGNHRLLLIALTYSLLINVFCVFAENPFQLGLLRFMYGFGTGALLPSVNSLLTKLT
                     PKEGISRIFSYNQLFNNLGSVVGPMMGSAVAAHMSYDWVFYLSSGLVLFNLIWSLTNF
                     RNYLKVRDV"
     gene            20660..20809
                     /gene="rpmG"
                     /locus_tag="VCA20_000188"
     CDS             20660..20809
                     /gene="rpmG"
                     /locus_tag="VCA20_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEP1834062.1"
                     /translation="MRVKINLQCSECGSKNYLTSKNAKTHPDKIEVLKYCPKERKVTL
                     HLETK"
     gene            20856..21089
                     /gene="secG"
                     /locus_tag="VCA20_000189"
     CDS             20856..21089
                     /gene="secG"
                     /locus_tag="VCA20_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEP1834063.1"
                     /translation="MYQALLAILLILSVILIVVIFIQPAKNQSSNVFDSSSGALFERT
                     KARGFEAVMQRITAILVFLWMLDALALVIISSK"
     gene            21310..23676
                     /gene="rnr"
                     /locus_tag="VCA20_000190"
     CDS             21310..23676
                     /gene="rnr"
                     /locus_tag="VCA20_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEP1834064.1"
                     /translation="MKNEIINYIKEVGPVTMDQLVDQFGASSAKSFTDLVKLVSSMEG
                     SRQLVFDNAGRIALPVPKVSKNKVTLQGIFRAHKSGFGFVTIDEEEDALFVSRDDVNF
                     AIEGDRVEIAIKKVADRLKGTAAEAEVIDILEHSLKTAVGLIVFDEDKPEYAGYIKSK
                     NQKIAQKIYIKKSPLVLTGTEILKVDIEAYPNKKRDHFIATIRDVVGHKDDVGIDVLE
                     VLESMDIVSEFPDEVLAEANRVPESPSEKDFEGRLDLRDEIIFTIDGADAKDLDDAVH
                     IKQLKNGNLELGVHIADVSYYVTEGSALDQETVKRGTSVYVTDRVVPMLPERLSNGIC
                     SLNPNVDRLTQSAIMEIDRKGKVVKHWIGQTVIKTTFRMTYSDVNDMIAGNKEKLATY
                     KAIVPSVELMVKLHETLETMRYKRGALNFDTTEAKIIVNKDGLPVDIQLRQRGIAERM
                     IESFMLAANECVAEHFAKLDLPFIYRIHEEPKSDKLQKFIDYATSFGLTVYGTASSIS
                     QDALQDLMERVKDEPYADVLNMMLLRSMQQARYSEHNHGHYGLGAEFYTHFTSPIRRY
                     PDLLVHRMVREYGHNPVEKAEHFEQVIPELAKSSSSLERRAIEAEREVEAMKKAEFMQ
                     EFVGQEFDGVVSSVVKFGLFVELPNTVEGLIHITNLNEYYQFHERTLTLQGEKSGRVF
                     RVGQPIRIKLTRADKMTGEIDFAHVPSELDIVEKALKAKRRTTSHSNRDRDDRSGRGR
                     GKHYKQEAAGRDSGRHKSGKDHKSKSGKKDKKKKPFYKEVTKKNKKRR"
     gene            23679..24146
                     /gene="smpB"
                     /locus_tag="VCA20_000191"
     CDS             23679..24146
                     /gene="smpB"
                     /locus_tag="VCA20_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEP1834065.1"
                     /translation="MAKGEGNVIAQNKKARHDYTIVDTVEAGMVLTGTEIKSIRAGRI
                     NLKDGFAQIKQGEAWLVNVHIAPYDEGNIWNQEPTRTRKLLLRKKQIESLGNEVKGTG
                     MTLVPLKVYIKDGFAKVLIGLAKGKHDYDKRESIKRREQDRDIKRTMKAINSR"
     gene            complement(24232..25274)
                     /locus_tag="VCA20_000192"
                     /pseudo
     CDS             complement(24232..25274)
                     /locus_tag="VCA20_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833743.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
     gene            25430..26290
                     /gene="tehB"
                     /locus_tag="VCA20_000193"
     CDS             25430..26290
                     /gene="tehB"
                     /locus_tag="VCA20_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEP1834066.1"
                     /translation="MTVLVPYKRMPIWNQETVPQHFLTKHNTKVGTWAKIKVLRGQLK
                     FEPISDDNEILGEYVYGPTDDIPFVEPQAWHRVTLLTEDTEFFLEFFCQPEDYFAKKY
                     DYTRTHSEVLEALKRIEPCKVLDLGCGHGRNSLYLAQQGFEVTAVDKDVPSIRTLLQV
                     KEVEDLDLQAGTYDINSASLEQDYDWIISTVVFMFLERDRVPAIIRNMQEQTRPGGYN
                     LIVAAMDTEDAPCPMNFSFTFGAGELKEYYRDWELVKYNEDFGQLHKRDENGNFLKMR
                     FATILARKNV"
     gene            26483..28387
                     /locus_tag="VCA20_000194"
     CDS             26483..28387
                     /locus_tag="VCA20_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HEP1834067.1"
                     /translation="MKKTFVRIATFLMLVCLFPAQLVQACSGFIIGKGLTTDGSILYG
                     RTEDYPYPPDNGAHNKNYIVVPAATYTEGDMLVDETFGFTAPHLANEFKYTSTPDAAR
                     GDGSNGNFGAHGFNEKGVSMTATVTAIPNKKVLAVDPLVTAGGLGEAILIDYVLPRVT
                     SAREGIELIAKTIDEKGSAEGNIIVIADKNEVWYMEILSGHQYVAIKFPEDKYAIFAN
                     TYYLGHVDFTDTENVIASAKVEEVAKQAENYMMVDGKFHIAKSYGPENYADGDRSRTY
                     AGIKLLDPVSPVTYEDAVYDLLRQPTDPSRRFSLQDTFALQRNRFEHLPEFRPDDEAG
                     KVKQGDNGANDQAADATYKYALGNENVIDAHVYQINSSLPSAFGGTVWLGLAQTRNTP
                     YVPFYGIVTDTYEAFKNRSASYDTNSWYWTVQNIDKMAISHPELFGTSIQEKWIALEK
                     EWIASQAALDAQYAGLSEDAAVALAPTVTEETLARSAEIFAQLKAVEAEMMAKIEAAT
                     TPSSSSTDSSSSTEPSTSTEPSSSSTEPSTSTEPSSSGTENSTSTSTSTSSSTSQSTS
                     ASDTGGVTDTSSSRGIVVSSDKKVTPTNKKGKSSLPSTGEQVSLLLVALGVAGILTAI
                     FLHRKKSSRE"
     gene            28508..30958
                     /locus_tag="VCA20_000195"
     CDS             28508..30958
                     /locus_tag="VCA20_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEP1834068.1"
                     /translation="MKQASYPIQGMTCASCALTVEKAVGKLAGMEEVSVNLATEKLSV
                     SYDEKLLGLEDIRQAVEKAGYQLVDNLVTESYDISGMTCASCALTVEKALDKLEGVEE
                     VSVNLATEKATIRYSRDRQNSASLERAVEQAGYQLIRPEEVEEAADKGPSKEEKLWYR
                     FAWSAAFTLPLLYIAMGPMLPWGGLPLPALLHQPLVYAISQVILLIPILYIGRSFFQK
                     GFKTLLQGHPNMDSLIAVGTGAALVQGLLMIVFLLMGKEVAMHGHHPELYFESAAVIL
                     TLITLGKYFEARAKGQTSEAIKKLMDLAPKTAQVLRNGQEIQVPIEEVVVGDQVIVRP
                     GQQIPVDGQVLEGQTRVDESMLTGESLPVKKALGDNVFGGTLNQQGAITMRATKVGRD
                     TTLAQIIRLVEEAQGSKAPIAKLADQVSAIFVPVVMGLALLSGLAWYFLGQESWIFSL
                     SIIIAVLVIACPCALGLATPTAIMVGTGKGAENGLLFKSGQAIETLQGVNTIVFDKTG
                     TITEGKPQVTDIHLLSTKNREQVLQLAASSEQFSEHPLAQALLQAAWTEKIVLLPATD
                     FQALSGRGLSVIIAEQTIYLGNERLMREQGIDVSKGRAVAEAFAQQAKTLVFLASQQE
                     VLAVIAIADKVKETSRQAIQALQAMGLEVVMLTGDNEKTAQAIAKEVGIEQVVSQVLP
                     DDKANQVKFLQEQGKTVAMVGDGINDAPALAQAHVGLAIGSGTDIAIESADIVLMHSD
                     ILDVFKAVKLSQATMRTIKQNLFWAFAYNVIGIPIAMGLLHVFGGPLLNPMFAGAAMA
                     LSSVSVVLNALRLKTYKL"
     gene            31037..31522
                     /gene="tpx"
                     /locus_tag="VCA20_000196"
     CDS             31037..31522
                     /gene="tpx"
                     /locus_tag="VCA20_000196"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEP1834069.1"
                     /translation="MTTFIGKPVTLVGPQLRVGDTAPDFVLMANDLSLKSLKDFGKQT
                     KVISVVPSIDTGICDTQTRRFNQELADRDNTVVITVSADLPFAQKRWCGAAGLEDAIT
                     LSDYYDHAFGKSYGVLMREWNLLARAVFVLNADNEITYVEYLDNVNEHPDYDAALEAV
                     K"
     gene            31617..31952
                     /locus_tag="VCA20_000197"
     CDS             31617..31952
                     /locus_tag="VCA20_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HEP1834070.1"
                     /translation="MEKTQIIQDVLALIQDLNRAFQADKVGTADEQRFQHNLTETTRI
                     LSEAKSVTNTELIAIEKFYRSTSFLVGLGDLRLSETSRQAWRAFDRYYYQTIREELKL
                     YGGSAIAQV"
     gene            32041..32742
                     /locus_tag="VCA20_000198"
     CDS             32041..32742
                     /locus_tag="VCA20_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019795669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1834071.1"
                     /translation="MSYIQVKNSSKYYQMGDTTIVANDGVSFEIEQGELVIILGSSGA
                     GKSTLLNILGGMDSNDEGEVWIDGVDIAKLSSHELTNYRRDDVGFVFQFYNLVANLTA
                     KENVELAAEIVKDALDAEEVLEQVGLGHRINNFPAQLSGGEQQRVAIARAVAKKPKIL
                     LCDEPTGALDYQTGKQVLQILQDMSRKQGATVIIVTHNSSLAPIADRVIRMRDARVHS
                     VELNADPQDIASLEY"
     gene            32742..36119
                     /locus_tag="VCA20_000199"
     CDS             32742..36119
                     /locus_tag="VCA20_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEP1834072.1"
                     /translation="MGETMKKKIYWKDMRQSLLSSKGRFLSIFSLMMLGALVLTGLKV
                     TAPNMEKTAQAYIASHRTMDLAVISGLGLSQADLDELETIEGATLEAGYFKDVVTNEG
                     QTAIRLFSAPQNLSTYKLVEGEMPSQKGQIALSASLKNRYKLDDTVQVTESDKDGKIL
                     TQTSFTLVGFVASAEIWDNETMGMAASGDGQLGGYGLVSQDIFKSEVYTIARIRYDDL
                     VDLPYYSQAYQDRLDQHQEDLEKLLANNDEQRLATIQSDGQTDISKGEEEIAQAQSQL
                     EQAESELETGEEQLATGRREFARGRAKVVQTEAELRAAQAQLLKNKFELEESKKELDG
                     RKARLEQTASFLDGYQMELETSAQQLEEAKQELDSQNAQLSQTASQISTGRASWFQAQ
                     QELDLEIANHLQEGQTLSDYPDLLARQEGLDVEKSRLDQMESAHEQANQAYLQGYDYY
                     QTKQNEYNSNLAQYQTWNQEYQAGLARYQAGLSQYEQGIADYNKGVEDYEWGLSQLES
                     SNQLLRQEELRLEEADKELSQAQSQFSEKKATADQEISQAQTEIAQAKSDLSKLEKAP
                     YQVYTRSSLPGGDGYTTYSNATRSIAAVGNVFPVVLYLVAALVTFTTMARFVDEERTQ
                     SGLLKALGYTNRQIMAKFILYGLAAGLVGTIVGIIAGNLLLSPLISNIITQTTVIGPA
                     KLHFYPLWTGLALLLSLASSVLPAYLVARRELTEKPAQLLLPKPPVTGSSIWLEKWPA
                     IWSRLSFTHKVTARNIFRYKLRMLMTIFGVAGTVALLFGGLGIRSSISGVVQRQFGEL
                     IHYDMLVVENSRATEEELDKLTHFLQSNQVRQSLPVAFEQLNQTVDENGQRKNLSISL
                     YISDRRDFGNQVSLESSTGQPIKLSDRGIVLTEKLAQIYGVSVGDKLSLTLEDKEVSV
                     RVEAVADMYAGHFIYMTDSYYEQVTGKQKTANAYLVQLKDRQLGHIQTLASQLLAMPA
                     VRSLVQNTSLIDMLTTIAGSLQTIMTILVILSILLGLVILYNLTIINMSERIRELSTI
                     KVLGFHNKEVTMYIYRETIALSLIGMLVGLVSGIYLHKLLLAMIGSDSIRFNPSVGLE
                     VYLIPILAISGILAALGWYVNHHLRKVDMLEALKSVD"
     gene            36419..37744
                     /locus_tag="VCA20_000200"
     CDS             36419..37744
                     /locus_tag="VCA20_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834073.1"
                     /translation="MCYNHLMKKISDLGLTGRKLVGEGLILVFIGIGFLIAGWQFPGL
                     ILRFVHAGLFFLALYELSMIFFRKKKSSESVIALVGKAVLFGILASLDLAVQIPLYFA
                     AIFVGIYQLFTAVINFITFYLYRKDGVQPRIRYLIDGIWLSLLGSASLFVSGTQLVVQ
                     TIVIGGYLILYGLTNLRDGFLFEEAIEQQNLKRHVRLPLPLFLAALIPRMTLQKVNDY
                     LADNEGQTAQSIYNRHKEIAELPALEVFVHVGEEGFGAVGHVDLSYKGQVYGFGSYDV
                     LSERLGGAIGDGVLFKAERQAYIDFCNQERMTMLGYQLALSSEQEKAVETRLAEIEGL
                     LLPWQPSAEKVSRRPDGQPIEMYAYRMKEAIGAALFKFKKSKFKTYFVLSTNCVLLAD
                     SVIGQAGTDVLGLRGFIAPGTYQSYLDQEYEKPHSLVVAKNIYYRKEKS"
     gene            37820..40456
                     /gene="polA"
                     /locus_tag="VCA20_000201"
     CDS             37820..40456
                     /gene="polA"
                     /locus_tag="VCA20_000201"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEP1834074.1"
                     /translation="MKKNKLLLIDGSSVAFRAFFALYNQIDRFKSPTGLHTNAIYGFN
                     LMLDHMMKRIEPTHILVAFDAGKTTFRTEMYADYKAGRAKTPDEFREQFPFIRQMLDA
                     MGVKHYELDQYEADDIIGTLDKMAERTEISFDVTIVSGDKDLIQLTDENTVVEISKKG
                     VAEFEEFTPAYLMEKMGITPTQFIDLKALMGDKSDNIPGVTKIGEKTGLKLLTEFGSL
                     DGIYENIDSMKASKMKENLIADKEQAFLSRTLATIDTNAPIEIGLDDIVYQGPKLEVL
                     GQFYDDMGFKQLRAQLGTTSSQEEAVLDFQIVTEISPAMLKQDRFFYFEILGENYHRE
                     DLVGLAWGDKEKIYVGGPELLDSPVLRDFLENQTIKTYDFKRGKVLLDRKEITLPPAT
                     FDSRLAKYLLSTVEDNSLTTIANLYGQTSLVPDEAIYGKGAKLDLPEREIFFPHLARK
                     VQVLIETEEPMLTKLEENQQLDLLFDMELPLANVLAKMEIAGIKVEAETLKAMQSENE
                     VLIDQLTKEIYELAGQEFNINSPKQLGTILFEEMGLPLEYTKKTKTGYSTAVDVLERL
                     APIAPVVSKILEYRQITKLQSTYVVGLQDAILEDGKIHTRYVQDLTQTGRLSSTDPNL
                     QNIPVRLEQGRLIRKAFVPSLENSVLLASDYSQIELRVLAHISQDQHLIEAFQQGADI
                     HTSTAMRVFGIEKAEDVTPNDRRNAKAVNFGVVYGISDFGLSNNLGITRKEAKAYIDT
                     YFERFPGIKNYMETIVREARDKGYVETIYKRRRELPDINSRNFNVRNFAERTAINSPI
                     QGSAADILKVAMINLDKALTEAGLATRMLLQVHDEIVLEVPVAELETVKAMVKETMES
                     AISLSVPLIADENEGSTWYEAK"
     gene            40443..41225
                     /locus_tag="VCA20_000202"
     CDS             40443..41225
                     /locus_tag="VCA20_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEP1834075.1"
                     /translation="MKQSKKGKSMKKLILCIGLCSLLLAGCGKASQKTEKTPASSGEE
                     FSTTLPILQEKQDTTNEQFNVLANAEVVAVNSTPPADETQKGHKIYAANNGVAEKDEN
                     GNIKEYFRYYDVPSTWTINVENTKDETVAAVYDVKEGGSNYMVQLYNINAFNQSPLEE
                     GRNMTPEELEARMLETNHSFNEKTVVTINGQEWQVGRQLLTDQKLARLTFYRMESTAA
                     YDDSVVVGSIYYSLDPGDDKKRTNLKKVIGQVKDVVYQIAKK"
     gene            41345..>41772
                     /locus_tag="VCA20_000203"
     CDS             41345..>41772
                     /locus_tag="VCA20_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002266707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEP1834076.1"
                     /translation="MSYSFQNPSQDSIFDYLKNAKTIAVVGLSSREETAAYRVSKLMQ
                     EAGYKIIPVNPKVAGGTILGELVYSSLAEIDQPIDIVDVFRRSEFLPEVAQEFIQSNA
                     KVFWAQLGLESQEAEKLLRQAGRNDIVMNKCIKIEYLEMK"
BASE COUNT        12489 a         7813 c         9614 g        11856 t
ORIGIN      
        1 cgctccctat ttccaacttc aaacactcca ctggattgtt tgaactcgct ttgccgtact
       61 ccgcgaaagt gacttgcttc gcatgtctta tttccaacct agaatagcct ataggctgtt
      121 ctagcaacct gtagctcgtt gccttgtact aaaagcgaac taaacgatta taatatctag
      181 ttatcattgc aaatatttat aagcgacaat gtgtatgaaa aactgacaga aattcagaaa
      241 aaacagaaaa tgaataggtt gtttacagaa acgttccgga aattgttgga aaaacaacaa
      301 atagaggggt gtaatctgtt ttttgtctgt tttctgtctc aaggagtctt gacaacttat
      361 ctaaaaaggt gcagaatgaa ctcgcaaacg tttgtttttt taataccatt tttatgtcta
      421 ttcatttcat tagtgaaaac atgaaaggat aaaacttatg agaaaaagtt cgcacttgat
      481 tttaagctca atagtcagtt tggcactcgt aggggtcaca ccattgagtg ttcttgcaga
      541 ttccaaacaa gatattaatc agtattttca aagcttgact tacgagccac aagagattct
      601 tacaaatgag ggagaataca ttgataatcc gccagcaaca actggaatgt tagaaaacgg
      661 acgttttgtg gtacttcgca gagaaaagaa gaatattacg aacaatagtg ccgatattgc
      721 tgttattgat gctaaggccg caaatattta tccaggtgct ttattgcgtg ctgaccaaaa
      781 tcttctggat aataatccaa cgcttatcag tattgcgcgg ggagatctga cgcttagttt
      841 gaatttacct ggtttggcca atggggatag ccacactgtt gtaaattctc caacaagaag
      901 tactgttcga acaggggtga ataaccttct gtctaaatgg aataatacgt atgctggaga
      961 gtatggcaat acccaagcag agcttcaata tgatgaaaca atggcataca gtatgtcaca
     1021 attaaaaacg aagttcggaa cctcttttga aaaaattgca gtaccattag atatcaattt
     1081 tgatgccgtg aattcgggtg aaaaacaggt tcagattgtt aactttaaac aaatttatta
     1141 tacagttagt gttgatgaac cagagtctcc aagcaagctt tttgcagaag ggacaactgt
     1201 agaagatttg caacgaaatg ggataacaga tgaggtacct cctgtttatg tttctagcgt
     1261 ttcttatgga cgctctatgt tcatcaagtt agaaactagc agtaggagta cccaagttca
     1321 agccgcattt aaagcagcca tcaaaggcgt tgatattagt ggcaatgctg agtatcaaga
     1381 cattctgaaa aatacttcat tctctgctta tatttttggt ggggatgcag gcagcgcagc
     1441 tactgttgtg agtggaaata ttgaaacact gaagaagatt attgaagaag gtgcaagata
     1501 cggaaaactc aatccaggtg ttccgatttc gtattcaacc aactttgtta aagacaatag
     1561 acctgctcaa attttgagca attcagagta catagaaaca acttcaaccg tccataatag
     1621 tagtgcattg acattggatc attcaggtgc ttatgttgcg aaatacaaca ttacttggga
     1681 agaagtatca tacaatgaag ctggagaaga agtttgggaa ccaaaagctt gggataagaa
     1741 tggtgtaaat ctgacctcac actggagtga aaccattcaa attccaggaa atgctcgcaa
     1801 tcttcatgtc aatattcaag aatgtacagg attagcatgg gagtggtgga gaacagttta
     1861 tgacaaagat ttaccacttg ttggtcaacg taaaataacc atctggggta caacgttata
     1921 cccacagtat gcggatgagg tgatagagta atctggcaat gtattatact ctcttagcca
     1981 cagaggctag gagctaaaca ctgttcgtca gaaacttatc acaagaaaat ggtattaaaa
     2041 taaggattct attcaaattt tgggtagaat cttttatatt ttcctaaaaa ctacttttag
     2101 gtatgagaac tcgttctgaa actattttca agaatttttg tatttctaca aaaatcgaaa
     2161 ttacattcta aataaaaaga ggttattgaa aaaataataa tttactatat aataaatagt
     2221 gtaagcgcta tctcggatcc gatagtagaa cggtgcaagg agaactaaat ggcaatcata
     2281 agcaaagaaa tcttaaaaga gcagattaaa gatggcatta ttgtttcctg tcaggcctta
     2341 cctggtgaac ccttgtatag ggaagaaggg gggattatgc ctttacttgt aaaggcggca
     2401 gaagaagcag gtgctgtagg tattcgagca aacagtgttc gagatattaa agaaatcaag
     2461 gaagtgacca atttaccaat tatcggaatt atcaagagag attaccctcc tcaagaacct
     2521 ttcataacgg caacgatgaa agaggttgac gagctagctg agctagacat tgcagttatt
     2581 gccttggatt gtaccaaacg ggagcggttt gatggtctgg acatcgttga tttcatcaag
     2641 caaatcaagg aaaaatatcc acaccaactc ttcatggcag atatttccac ctatgaagaa
     2701 ggtgtgacag cctacgaggc cggaattgat tttgttggaa cgaccctttc aggctacaca
     2761 tcctatagcc gacaagaaga tggaccggat atggagctgg ttgaccgact ttgtcgtgct
     2821 gggattgatg tcatcgcaga aggaaaaatc cactatccag atcaagctag gaccatccac
     2881 gatttaggtg tggcaggtat cgtcgttggc ggagccatta cccgtcctaa agaaatagca
     2941 gagcggttta tccaagcgat tgaaaaattt tatggaggta aaaatgactg agttaaaatt
     3001 aaaacactta tttaaaaagt atccaaatag tacatattac tcagtagaag actttgactt
     3061 agaaattaaa gataaagaat ttatagtatt tgttggcccc tctggttgtg gaaaatctac
     3121 gacattgcga atgattgctg gactagaaga tattactgaa ggagagttgt atataggtga
     3181 aacgcttatg aacgatattg cacctaaaga tcgtgatatt gctatggttt tccaaaacta
     3241 tgctttatat ccacatatga ctgtttatga caatatggcc tttggattaa aacttcgcaa
     3301 atactcaaaa tcagaaattg aaaatcgtgt taacgaagcg gctgaaattc tgggattaaa
     3361 ggaattttta aatagaaaac ctgctgactt atcaggtgga caaagacaac gtgttgcaat
     3421 gggaagggct attgttcgtg atgctaaagt atttttgatg gatgagccgt tatcaaatct
     3481 tgatgcaaaa cttcgagttt ctatgagaac tgagattgca aaaattcatc aaagaattgg
     3541 ttctacaact atttatgtca cgcatgacca aactgaagct atgacattgg cagacagaat
     3601 tgtaattatg tcagcgacaa aaaatgaaga aggtacaggg acaataggaa aaattgaaca
     3661 agttggttcg cctcaagaac tttataatta tcctgtaaat acttttgtag ctagctttat
     3721 tggttctcca gccatgaact ttataaaatt atttgttggt gaatcaaata tttataatga
     3781 ttacggattt gacttggaat taccagaagg ggttaaaaaa gttttgtctg agaacggata
     3841 tattggtaaa gaaattatct ttggaatccg tccagaagat atctctgcgg atttaatttc
     3901 atcaactact tttccaaatg caaccatcga aactgaagtt gttgtttcag aattattagg
     3961 tagtgattca atactatatt gtaatacaaa taatattgaa ttttctgcga gagtagcggc
     4021 acacgatcat cgtcaagcag gtgaaaaaat tagattggca tttaatatat caaaaggtca
     4081 tttctttgat atacagactg aaaaaagaat tgggtgacat agaatatgat aattacaaat
     4141 atattaaatt tagaaagata taattcgata aatcctcaat tttggaaatt acaaaaattt
     4201 atagaagaaa atgacttagt aaatttacct gagggtaaaa tagaaattga tggagaagaa
     4261 atattcggta attgctttag ttatatagct gatggtaaac cgggtgaatt ttttgaaaca
     4321 catcggaaat atatagatat tcatttagtt gtaaacaaca ttgaagcaat ggcaacatca
     4381 tcaacaaatt gtacatctgt aacaagtccg tattgtgagg agaaagatat cgaattattt
     4441 gaaggagaaa ttgagcaaat cataaaatta aatgcaggaa catgtttaat cacatttcca
     4501 gaagacctac atcaacccaa gattggaata aatgaatttc cagtgaaaaa gatagttttt
     4561 aaggtaaaaa tttgaaaaag aacaggagaa tgatatcatg tttaaaataa aaaaaattat
     4621 caaacatgct gggctaatat taacagcaat aggcttggct gcgtgttcgt caggtagttc
     4681 tgcaacaaat atagcacctg aaaaaaacgg tgaattagaa ggtgaaattg taatgtggca
     4741 ctccttcact caaggaccgc gtttagagag tattcaaaaa actgcagatg aatttatgga
     4801 agagcatcca aaggttaaaa taaaaattga aacattttcg tggggcgact tctatactaa
     4861 atggactact ggtattgcta atggaaatgt accagatatt agtactgctt taccaggaca
     4921 tgtaatggag atgataaatt cagatgcaat tattccgttg aatgacagta ttgaagccat
     4981 cggaaaagat cgatttaatg aaacagcact taatgaagca aaaattggta ctgactatta
     5041 ttcaattcct ctatattctc atgctcaggt aatgtgggtt cgtacagatc tgctgaaaga
     5101 aaaaaatatt gaagttccta aaacatggga tgagctttat gaggcaagta aaaaattaac
     5161 agaaggcgat gaatatggaa tgtctgttcc attcggtact aatgatttga tggggactcg
     5221 tttcctaaat ttctatgttc gaagtggtgg aggaagttta ataacaaaag atctgaaggt
     5281 tgatttaaca agtaaattag ctcaagacgg aattaagtat tgggtgaaaa tgtataatga
     5341 agtatcacca aaagactctt taaactttaa tgtattacaa caagctacat tattttatca
     5401 aggaaaaaca gcttttgatt ttaactcagg gttccatatt ggaggtgttg aggctaacag
     5461 tccacaatta attgattcta ttgatgccta tccaattcca aaaatcaatg aatctgacga
     5521 aacaaatggg ttagaaacat ccaacattcc aatggtagtc tggaaaaatt caaaacaccc
     5581 agaaattgct aaagctttct tggaatccct atatgaggat gaaaattact tggaattttt
     5641 agattcaact ccagttggta tgttgcctgc tataagtgga attactgatc taccttctta
     5701 tcaagaaaat gaaactcgta aaaaattcaa acatgctgaa gaagtgattt tagaagctgt
     5761 gaaaactggt acagctattg gttatgaaaa caaaccaagt gttcaagctg gtttactcac
     5821 caatcaacat gtaattgaac aaatgttcca agagattatt actaacggaa aagacccaat
     5881 ggaagctgca aaagatgcag aaaaacaatt gaatgatttg tttgattcag tcgcggtcga
     5941 tgttgagtaa taattcccga aatttaagtt gaagggagta tgttatggtc aaagatagaa
     6001 atttgactag gtggattttt gtgctaccgg caatgataat cgttggatta ctatttatat
     6061 acccattttt ttctagtata ttttatagtt ttacaaataa gcatttaatc atgccgaatt
     6121 ataaattcgt aggatttgac aactatataa aagtcctatc cgatcccaat ttctttaatg
     6181 cattctttaa ttctttaaaa tggacagttt tctcattaac aggtcaagtc ttagtaggtt
     6241 tttgtcttgc attagctcta catcgaataa gaagatttaa aaaattatat agaacattgt
     6301 taattattcc ttgggctttt ccaacgattg taattgcatt ttcttggcaa tggatactta
     6361 atggcgtata cggttatttg ccaaatatga ttgtaaaatt agggcttatg aacgttgctc
     6421 cagcatttct aacagatcga acttgggcct tcctttgtct tgtatttatt aatgtttggt
     6481 ttggagcacc aatgattatg gtaaatattt tgtcagcttt acaaactgtt cctcaagaac
     6541 aatttgaagc ggcaaagatt gacggcgcat caactttaca agtatttcga tatattacct
     6601 ttcctcatat caaagttgta gtaggcttgc tagtggtgct ccgtactgtt tggatattta
     6661 ataactttga cattatatat ttgataaccg ggggaggacc ttccaatgca actatgactt
     6721 taccaatatt tgcatataat ctcggatggg gaactaaatt gttaggaaga gcctcagcag
     6781 ttacagtttt actctttatt ttcttactag caatttgctt tatttacttt ggaataataa
     6841 gcaaatggga aaaggaagga ggtaaataat gaatgcaaaa aaaggaataa tcctagatgt
     6901 tatttcacat ttattcttag cccttgcaac tatagtttcc atatttccat tagtctggat
     6961 agttatttcc tcagttaagg gcaaaggtga gttgacacaa tttccgacac gattctggcc
     7021 ggagcaactt actttagatt attttacaca cgttattaat gacttgaatt ttattgtaaa
     7081 cattagaaat tcattagttc ttgctttgtc tgcaacagtt gttgcaacca ttatttcctc
     7141 aatggcagca tatggaattg ttcgattctt tccaaaacta ggagcgatta tgtctagact
     7201 cttagtaaca acttatatat ttcctccaat attattagct attccatatt caattgcaat
     7261 tgctaaagct ggattaacaa atagtttgtt tgggttaatg ctaatctatc tttcatttag
     7321 tgttccatat gccgtatggc ttttagttgg tttctttcaa acagttccaa ttggaattga
     7381 agaagcagct agaattgatg gagcaaataa gtttgtgact ttctacaaag ttgtattacc
     7441 tatagtagct cctggaattg tagcaactgc aatatataca tttatcaatg cttggaatga
     7501 atttttatat tcactaatta tgataaatga tactagtaaa atgactgttg cagtagcact
     7561 tcgatcacta aatggatcag agattcttga ttggggagac atgatggctg cctctgttat
     7621 agttgtttta ccatcaatcg ttttcttctc aattattcaa aataaaattg caagtggttt
     7681 aagtgagggg tctgtaaaat agaaaggaat aaaatgaaga atattaaatt actaaaaaaa
     7741 tttagtattt gtcttttagc tacaacaatg ttaacaactt tacaccctat tttcattaat
     7801 gctgaagacg caattgggac tgaaattgta aatggatctg aataccattc aggcaatttt
     7861 actttagaga atagtagaat tgacattaca aatgaagctt ttgataaatt aaacactgaa
     7921 tctcaaacta ttgtattgaa atttcaatca gacaatccaa attcactcca agcatttttt
     7981 ggggtttcaa atagtaatag tggatttcga aataactatt tttcaatatt tatgagagat
     8041 accggtgaag ttggttttga agtccgtgat aaaaataaag gtattaatta tttatttagc
     8101 agagctgctt cgatttgggg tattcataaa ggtcaggctg tagaaaatac gatagtcttt
     8161 acatcagata atgaatcaaa aacttattca ttatttgtta atggtacaaa agtattatca
     8221 gaaactgttg aaaacttttt acctattaca tctattgaag gagttgataa tgtttctgta
     8281 ggcggtgtaa atcgagaagg taaagatgcc tatactatga gaggacaagt gaatcaattg
     8341 tcaatttata atagagtact ttcaattgat gaaataaaca gtaagtttgg agaactgcca
     8401 taccaattca ttttccaatc aggagatgcg acaaacgcaa actatttcag gatccctact
     8461 ttatatacac ttagcaatgg tcgtgttcta gctagtattg atgctagata tggaggaact
     8521 cacgactcta gaagtaaaat aaatattgca acttcatata gtgatgataa cggaaataat
     8581 tggtcaattc catctttgcc attaaaattt gatgattacg cagagcaatt gattgaatgg
     8641 ccaagatcag gtaatctaaa aaatgtacag attaatggaa gtgcttcatt tattgattct
     8701 gctttagttg aagataaaac aacaggtaat gtagttatga tcgctgatgc aatgccagcg
     8761 ggaatcggaa acaataatgc cgatagaaac gattccggtt ttaaagaaat tgatggtaaa
     8821 tattacttaa aactaaaaaa agatggggaa acaactttta attattctgt tcgtgaagat
     8881 ggtgtaatct atgatgatat cgctaactta ccgacacaat atagattaaa tgataaatat
     8941 gaagttttag aaggtgatgt tccactaaac gttgaacagt attcgtatag tttcgatgca
     9001 tctggattac aagaatttca taatggtaat ttagtaccga tgaacgtatt ttatcaagat
     9061 tcacttttca aggtagctag aaccaattat cttggcgtaa caataagtaa ggataagggt
     9121 gaaacatgga gtaactttaa actgcttcca cctatgctcg ggttgaatca taatgctacc
     9181 tatttgagtc ctggtcaagg attatcttta atgaatagca atcggattat ttttgcgacg
     9241 tatactaggg gagaattaac ttatttaatt tcagatgatg gtggagtaag ttggaataaa
     9301 tcatccgctg gagtaccatt ccaaaatgca actgctgaag ctcagatggt agaactcaaa
     9361 gagggtgtaa ttcgtacata cttccggaca acgacaggta aaattgccta tatgacaagc
     9421 tatgactcag gtgaaacatg gtcagatgtt tcctacctag atcatatttc tcagacaaac
     9481 tacggaaccc aagttactgc tataaaatat tcaaaaaaaa ttgaaggtaa agatgcaatt
     9541 attttgagca ccccgaatac aaattcaggt cgtagaggtg gtcaattgtg ggtaggtttg
     9601 attaacccag aggacgatac tgtcaattgg aaatattcgt ataatgtaga tattcctaca
     9661 tttggatatt cctactcagc attgacagaa ctaccaaacg gacatattgg attattattt
     9721 gaaaaatatg attcttggtc acgaaatgaa ttacatttat caaatgttgt tcagtatatt
     9781 gatttagaaa cctcagatat tattaagtaa acaatataag gataagtcta gtggtaaaaa
     9841 gtcaatagaa agttgattaa tttttagttg attttttaga aaacagggtg cactaaaaac
     9901 acctagcgaa aatcactttt ttcactagct cacctgccgt ctgtacgcta ctacgtacag
     9961 aagtgagcgt aatggaaaca gctagtgtgt tggaaatagg aacaacgctt gggtaacaga
    10021 cttaaaaatg aagcctaagc aacaaggaga aaaagaaaca accatgagtc cattccatga
    10081 ctctattgtt ctggaaaacc ttgcaatagt caagttaacg ttattacaat tattaaaaat
    10141 ttgggattga aacatcccca taagcttatt ttacgaggag aaataacatg attagatatg
    10201 gtgtagtagg tactggatac tttggagcag aactcgcacg attcttaata ctaaatgaag
    10261 gagcagtaat aactaaagta tatgaccctg aaaatgccgg accgattgct gaagaattag
    10321 gagcacttga aacacaatca ttagatgaat tagtttcaag tgacgatgta gattgtgtga
    10381 ttgtagctac cccaaattat cttcataagg aacctgtaat caaagctgca cagaatggta
    10441 aacatgtctt ttgtgaaaag cctattgctc tttcttatga agattgtaaa gaaatggttg
    10501 aaacatgcaa gcagtatggt gtaacattta tggcaggtca cattatgaat ttctttaatg
    10561 gtgtacaaca tgctaagaaa ctaatcaatg aaggagccat tggagatatt ttatatgtgc
    10621 atacggcacg aaatggttgg gaagagatgc aagagacaat atcttggaaa aaaattcgag
    10681 aaaagtctgg aggacacctc tatcatcata ttcatgaact agattgtgta caatttatca
    10741 tgggtggcgt acccgaaact gtaacaatga ttggcgggaa tgtagcacac gtgggtgaaa
    10801 actttggtga cgaagatgat atgatcattg tgaatatgga atttccaggg aaaaaattcg
    10861 cagtcttaga atggggatcg gcttatcatt ggggtgaaca ctatgtgttg atccaaggga
    10921 caaaaggtgc aattaagcta gacatgttca atacaaaagg tactctcaaa gtaaatggta
    10981 atgagactaa tttcttagtt catgaaacac aggaagagga tgatgatcgt acaagaattt
    11041 attatagtac agagatggat ggtgcaattg cttatggtaa gccaggcaaa cgcaccccaa
    11101 tgtggttatc atctatcatc aagaaagaaa tgaagttttt acatgaaatt atggagggtg
    11161 gaccagtttc ggatgaatac aaaaaattat taactggtga ggcagcactt gaagctatcg
    11221 cttcagcaga tgcttgtact gtctcattaa atgaagatag gaaagtaaaa ttaactgaaa
    11281 tcatgaaata atagaacagt taaatataaa atcatcagga taaacgtgaa tatcatacag
    11341 gaataataat actattcctg tatgatcata tttacaagaa agttaattta ttttatttag
    11401 aaaaggataa atagtatgac actagaaaaa tatcatggta ttatcccagc cttctatgcc
    11461 tgttatgacg aggctggtga gattagttca gaacgtgtaa aagccctggt tcagtatttc
    11521 attgacaagg gtgttcaagg gctttatgtc aatggttcat ctggtgaatg tatttaccaa
    11581 agtgtagcgg accgcaagca aattcttgaa gcggtcatgg aagttgccaa gggcaaattg
    11641 accatcatca accatgtagc ctgcaacaat ttgaaggaca gcgtggagct agcgcgtcat
    11701 tcggaagaat tgggtgtcga tgctattgcg gccatcccac ctatttactt ccgtttgcca
    11761 gagtacagca tcgctactta ctggaacgga attagtgcag ctgcaccaaa cactgatttc
    11821 attatctaca acatcccaca attggctggt gtttccttga caccaagtct atataaggaa
    11881 atgctgaaaa atccacgtgt ggttggggtg aaaaattcct ctatgccagt tcaggatatt
    11941 gatacctttg tcagtctagg cggtgaagac tacgtggtat tcaatggtcc agatgagcaa
    12001 ttcttaggtg gtcgcctcat gggcgctaag ggtggaattg ggggtaccta tggtgctatg
    12061 ccagaactct tccttcgttt gaaccaattg attgcggaca aggacttgga aacagcccgt
    12121 caattgcagg cgactatcaa tactattatc ggtaaactgg tatctggtca tggtcatatg
    12181 tacgctgtta tcaaggaagt catccgtatc aatgatggct tggacattgg ttctgttcgt
    12241 gaacctctga cagccttgac ggaagcagac ttgatcattg cccaagaagc aacccaactc
    12301 attcgcgatg ctagggaaag atttggtgcc taggaggtgt ctatgaaaac ctatcttgct
    12361 attgatattg gcggaactca gattaaatac ggtcgtctgg atgaagcagg tcagattctg
    12421 gaaagttata agatagacac ggaagcctac aagggtggcc cccatatcct agcgacagtc
    12481 aaaaaacttg tggaagactt tcatgcccaa gttgcccttt caggtgtagc tatttcatca
    12541 gctgggatgg ttgaccctga caagggagag attttctact caggaccaca aattccaaac
    12601 tatgctggaa tccagttcaa gtcagaaatt gaaacaactt tcggacttcc ttgtgagatt
    12661 gaaaatgatg tcaactgtgc aggactagca gaagggattt caggtgctgg tcagggcagt
    12721 cagatttcca tttgtttgac tattggcacg ggcattggag gttgcttgct agtggatggt
    12781 caaatcttcc atggctttag taattcagcc tgtgaggtcg gctatctcca cctttcagat
    12841 ggggctttcc aagacctggc ttcaacgacg gctcttgtcc aagaagtggc tcgtttacat
    12901 gatcagaatc cagaagagtg gaacggctat aagattttcc aggaagccaa ggcaggagat
    12961 gcccactgta ttgttgccat tgagcgcatg gtcaactatc ttggtcaagg tattgccaat
    13021 atctgctatg tagccaatcc agaagtggtc attcttggcg gtggtattat ggcgcaaaaa
    13081 gattatcttt atgagaaaat ccaagcagct cttaaggaaa atctggtttc aagtctagca
    13141 gacaagacca agctggcctt tgcagagcat gagaatgctg ctggtatgct gggagccttc
    13201 tatcacttcc aacagaagca agggctaaga taatatcagc taatgtaaga tggagagctt
    13261 cctccccttt ctcttctcct ttagatttgg aagctttccg tcttttgatt gttatagagg
    13321 tggaatgatg attgataata tgaccttgca ggatatgctg acctatcgtg gtcggcagga
    13381 agtccctgca gattttgatg ctttctggca gaatgaaaaa aatcagttga caggtctaac
    13441 agaccatgta ttacttgaaa aagaatgtgg cttgcctcat gtagcttgct acgaactgac
    13501 ctttacgggt acaaatggct ccaaggtttt tgccaagtgc ctctttccaa agagtgagca
    13561 agctgttcca gttctctttt actttcatgg ttaccaaggt caaggtcccg actggtctga
    13621 gaatttaaaa tttttagcag ctggttatgg ggtagtagct atggatgtac gtgggcaggc
    13681 tggtcgttct caggatttgg ggcaatttga gggaataact gttaagggac aggttattcg
    13741 agggatgctt caagggccag aacacttgtt ttataaggat gtctatcagc tgattgagat
    13801 tattgccagc ttggactttg ttgaccagaa gcgcctggtg tcttacggtg cctcacaggg
    13861 aggagccttg gccttggtgg cagccgctct cagtccctac atcagtaaaa ccgtagccat
    13921 ttatcctttc ctatctgact ttaaacgggt gttggaacta ggaaacacta gcgaggccta
    13981 tgatgaactc ttccgttatt tcaagttttc tgatccattt cacgagacag aagaagcggt
    14041 cttacagact ctttcctata ttgatgtgaa aaacttagct catatgatta cctgtcccgt
    14101 tgccatggtg actggcttgg aggatagtgt ctgtccacct tctactcaat ttgccattta
    14161 caaccgactg gaagtagaaa aagagatgaa aattttacca gaatatgcca cgatgccatg
    14221 catgtaaagg ttaatgacta tgtctatgat tatttaatcg ggacaagttt tttatctaac
    14281 taataaaggc actacttaaa tggtagtgtc ttttatcgta tgtttaaaaa tgttttcttt
    14341 tctttcttta tctatagcca aaaaataagc gtaaataatg tccaacataa tcaataatgg
    14401 aatttgtggc gaaatacggt taccataatt aaggtggcgg acagaggcga ctggaatgat
    14461 ttggtcaaaa gttggatggg gaaaactctt agttgttaac agggcggtag gaatcccctt
    14521 ttgagcagcc agctggagat gctccatgac ctcttctgtc tgaccagaca aagaaagacc
    14581 gataaccaag caggtctgat ctaggatatt ggttgtccaa atgaggttgt ttttatcagt
    14641 aattgcatca cagactacac ccagtcgcat aaagcgggat ttgatttcca ttgcaaccaa
    14701 accagaactg ccgattccat aaaaatagac acgcttacta ctgtcaatca gtttggcaat
    14761 ttcttccaat ttttcttcat caacgagttt attggtagca gttaggattt cctcatagtc
    14821 taccaagact ttttttgtta ggctccgctg taatttttca aaatgtttat ccaagtattg
    14881 tcggttattt tgaaaagcga aaacaaattc tcgataacct gagaaaccgc attttttagc
    14941 gaatctggtt agagctgatg gggaaatatg cagggtttgg gtggtgtttt caaggctgaa
    15001 atcagcactg gcagggtcca gttgggtaaa gtagctggca atatgttttt cgagccttgt
    15061 catctgttcc aaatgtgctt cgataattgc tgtcatgttt ttgggtgttt ctaacatctt
    15121 cattcctctg atagcttctg aggatattat atcataaaac ctcctcattg ataggtttta
    15181 tgatataatg gaaacgtaaa aggaatgtaa gattatgtat attgaaatga ttgatgaaac
    15241 agggcaggtt tgtgctcaaa tgcaggagca gattacggaa ttgcttcagt ttgctgccga
    15301 aaaaattggc aagcagaata aggaaatggc tgtgaccttt gtggacaata agcgagtcca
    15361 tgaagtgaac ttggagtacc gtgggattga ccgtcctaca gatgtcgtta gcctagagta
    15421 taagccagaa aacgagattt tcttcgacga ggaagacttg caggacaatc ctgaattggc
    15481 tgagatgatg gaagattttg atgtctatat tggtgagttg tatatttcta tcgaaaaggc
    15541 gcgtgagcag gctgaagatt atggacacag ctacgagcgc gaaatgggct ttttagctgt
    15601 acacggtttt ctgcatatca atggctatga tcattatacg ccagaagaag aagcagaaat
    15661 gtttggttta caagaagaaa ttttgacggc ctatggactt acaagagaat aattcaaaaa
    15721 atcgctggaa aaaccgagag ctgattgcca gtctagaatt tgcagtaaca ggtttattga
    15781 cggctttcaa ggaagaacgc aatatgaaga agcaccttgc ttcagctgtc ctagtggtac
    15841 ttgcgggact tattttccaa gtatctgtga cagagtggct cttcctcttg ctcagtatta
    15901 gtctggttat cgcttttgaa attgtgaact cggctattga gaatgtggtg gatttggcgt
    15961 cagactatca tttttctatg ttggcaaaga atgcaaaaga tatggcagca ggggcagtct
    16021 tgtttgtgtc aggatttgcc ttgttgacag gattgattat ctttgtaccg aaaatttggg
    16081 actttatttt ttagaatata aggagaatac atgacattta agtcaggttt tgtggcgatt
    16141 ttaggtcgtc caaacgttgg gaaatcaacc tttctcaact atgtaatggg gcaaaaaatt
    16201 gccatcatga gcgacaaggc tcaaaccacc cgtaacaaga ttatgggcat ttatacgaca
    16261 gaagaagagc agattgtctt cattgacaca ccaggaattc acaagcctaa gacggccttg
    16321 ggtgacttta tggtggaatc tgcctatagc acccttcgtg aagtggatac ggtcttcttt
    16381 atggtgccag ccgatgaaaa gcgaggcaag ggtgacgaca tgattatgga acgccttaaa
    16441 caggcaaaag ttccagtcat tttagtggtt aacaagattg acaaggttca tccagaccag
    16501 cttttggaac agatcgatga tttccgtcaa cagatggact tcaaggaaat tgtaccaatt
    16561 tctgctaccc aagggaacaa tgtcaaccga ctcatggaaa ttctcaagga aaacctggac
    16621 gaaggcttcc agtatttccc tgcggaccaa atcactgacc acccagaacg tttcttggta
    16681 tctgagatga ttcgtgagaa ggttctacac ctgactcgtg aggaaattcc gcactctgtg
    16741 gccgttgtca tcgagtccat gaaacgtgat gaatttaccg acaaggtcca tatccgagcg
    16801 acgattatga ctgagcgtga tagccagaaa ggcatcatca tcggcaagca aggagccatg
    16861 ctcaagaaaa ttggttccat ggctcgtcgc gatatcgaac tcatgctagg tgacaaggtc
    16921 ttcctcgaaa cctgggtcaa ggtcaagaaa aactggcgtg acaaaaaact cgacctcgcc
    16981 gattttggtt ataatgagaa agagtattaa aaacgtccgg gggagtgaaa cagtctagga
    17041 atagactgtt tcagctggtc acctagaaat gtgaaagtga ccatttgaaa aaccgagctt
    17101 gaaaactaga gggcgagatg gtctggtgtg acttgtctca ccagaattga atgaatgaat
    17161 ttcaagcgta aagcaccact tttgtggtgt tttgcatcgt tacggtgaga tattatgacg
    17221 attatagcag ttaatgaaaa gactctttct atctgggcta cttttgcaag tcaggtttgg
    17281 cagacaagtg agcaagtttt gatagagaaa ttttcaaata atgagtttcc ttttgagttt
    17341 ctgtactatt ctcaaagtga ggagccgata gcgtggatta gtttgtcgct ccgtcaggac
    17401 tatgtagaag gctgtcagac gagtccagtg gcctatttag aaggaatttt tattagtccg
    17461 aattaccgta gtcaagggat tgcggaagaa ctgttgcatt ttgcagaatg ttgggctaga
    17521 agccgtggta taagccaact ggggtctgat gcggaactag acaatctact gagtcagaac
    17581 tttcatatca aacatggctt ccgagaagtt agccgaacag ttcactatgt tcgaaatttg
    17641 aatattgaat aaaattatgt ctaacacaaa tttagttaag aaaggaaagc cgattaggtg
    17701 ttgtcttgaa ctcgcctacg actctttgaa aatcacaacc gtgacctagg tgccactgca
    17761 ccgtcggtca ggtttctatt tgctttgtaa ttttgttaga aaggacgagt gtttgcgtac
    17821 ttgaacccga gctaaaagct cggaaaatag acagttcccc ttgtatgcaa gtcacatagc
    17881 gtcgctcttc taattttact atatttagaa aggaaagccg attaggtgtt ggaattgaac
    17941 acgcctacga ctctgtgaaa aaagacaacc gtgacctagg tgccgaagca ccgtcggtca
    18001 ggtttctatt tttctttgag tcgcttaacg gcttagtatc ttagtatgcc cgaattgcct
    18061 gaggttgaga cggttcgtcg tggtttaaat cggttggtca aaggaaaggt catcgaaaag
    18121 gtcaaggtga cctacgctcc tatgattaag actggtgtgg atgccttctg tcaggacttg
    18181 cttggtcagg agattttaga tgtggctcgt cgtggcaagt atctcttgat ttacctgaca
    18241 gaccatgtcc tcatttccca cttgcggatg gaaggtaagt ataatttctt tcctgaccag
    18301 gtgcctgcca acaagcattt ccatgccttt tttacttttt cagatggctc gaccttggtt
    18361 tatcaggatg tccgcaagtt tggcacaatg gaactgctgg gcaaggcaga tgtggatgcc
    18421 tattttatca gtcgaaaaat tggtcctgag ccgacggagg aagattttga cttagaagaa
    18481 tttgccaaaa agttagctaa gtcgaaaaag cccattaagt cgcatctttt ggaccagtct
    18541 ttggtggctg gtttgggcaa tatctatgta gatgaggttc tgtttagagc acaagttcat
    18601 cctgctcaag caagtaatca gctatcggca gagcaggtgg cagacctccg tcaagctacc
    18661 atagaagttc tgcaactggg tattgaaaaa ggtggttcaa ccattcggac ctataagaat
    18721 gccttgggca tggacgggac tatgcaggat tatttacaag tttatggaaa gacaggacag
    18781 gcttgtcccc gttgccagat agagattgtc aaaatccagc tgggtggtcg agggacacat
    18841 ttctgtccca cgtgtcaggt gaaacatggc taaaattatc ggtttaacag gcggaatcgc
    18901 ctctggaaaa tcaatggtca cagctttttt gcgagaacag ggctatccag tcatcgatgc
    18961 ggatgcggtt gtgcatgagt tgcaggctaa aggcggtaaa ctctaccagg ttttgcttgc
    19021 cgagtttggg gaagcaatcc tgtcagctga tggtagtcta gatagggcta agctgggcca
    19081 ggctgttttt gaagatagca aattgcgggc tcgcctgtct gacctgcagg accaaattat
    19141 taggcaggag ctgttagata ggagggacgc cctcaagcag actgaggatg taattttcat
    19201 ggatattcct ttgctttatg aggcggatta tagcggggaa gtaaatgaag tctggctagt
    19261 ttatgtcgat agagcacagc agttggagcg gcttatgaaa cgtaatggct tagctgttca
    19321 agatgcagaa aatcgtctgg ctgctcagct ttctttagag gaaaaatggt cgcaagccca
    19381 ggtggtgatt gacaatagcg gagcggttga ggcgacctta gcacaggttg cgcaactttt
    19441 ggaggaatta aaggatggac gacggtagta gttattggaa gcagaatttg aaggtggctt
    19501 ggttgggaaa cttcctgaca gggacgagtt ttaccttggt tatgcctttt atctcggttt
    19561 ttgtggaaga gttgggggta ggtcctggac aggtagagta ttatgcggga cttgcggtat
    19621 ctgttaatgc ccttgcggca gccttgatgg cgcctatttg ggggagtttg gcggaccgtt
    19681 acggtcgcaa gcccatgatg gtgcgggctg cttttgcgat gatttttact atgggtggta
    19741 tggcctttgt accaaatgtt ttttggttgc ttgccttacg tgtcctaaac ggggtattta
    19801 cagggtacat tcccaatgcg actgccttga ttgccagtca ggttccaaag gataagacgg
    19861 ggtatgcgct ggggacctta tcaacaggtg cggtagcagg taatttgatt ggtccgaccc
    19921 tgggtggtat cttagcagag atgtttggcg tacatatggt atttttgctg gtgggacttc
    19981 tttatgctat cgttgtgtta ctgaccgttt tctatattcg agaggatttt gttccaatca
    20041 aaaaaggcga agagatgtcg gttaaagagg tatttgagca ggtgaaagat cggcagatgc
    20101 tggttgggct ttttgtgact tctatgatta ttattgcagc tgctcaggct gttgttccta
    20161 tattgaccct ctatgtgcgg catttgggac agacagataa tctgttattt gttgctggtt
    20221 ttatcatttc cttgcctggt atggcatcgc tagtgacatc aggttattta gggaaatttg
    20281 gtgatcgtat tggcaatcat cgcctcctgt taattgcttt gacttacagt ctgttgatta
    20341 atgttttctg tgtttttgca gaaaatcctt tccagcttgg tttgttgcga ttcatgtatg
    20401 gttttgggac aggagcattg ttgccttcgg tcaattctct tttgaccaag ttgaccccaa
    20461 aagaagggat ttcaaggata ttttcctaca accaactctt taataatcta ggttctgttg
    20521 ttggtcccat gatggggtct gcggtagctg ctcacatgag ttatgactgg gttttctatc
    20581 tgtcgagcgg acttgtcctc tttaacctta tctggtcttt gaccaatttt agaaactatt
    20641 tgaaagtaag ggatgtttag tgagagttaa aatcaattta cagtgttcag aatgtggtag
    20701 taagaactac ttgactagca aaaacgccaa gactcatcca gataagattg aagtccttaa
    20761 atactgccca aaagagagaa aagtaaccct ccaccttgaa actaagtagg ttttatggta
    20821 taatgtcata agttattgta gaaagtgaaa aaaatatgta tcaagcatta ttagcaattc
    20881 tgttgatttt atctgttatt ttgattgtag tgatttttat tcaaccggct aaaaatcagt
    20941 ctagcaacgt ctttgactct tctagtggag ccttatttga acgtacaaag gcccgtggtt
    21001 ttgaagcggt gatgcaacgc attacagcta ttcttgtttt tctttggatg ctggatgcgc
    21061 tggcacttgt tatcatttca agtaagtaga taaatcagac tgggacttgt tcttggtcta
    21121 tttttttggg agtgggccag aactcgacaa atgtaaagga gttcgtctgc ctacccccgc
    21181 acaagttgaa taggtatggt tcggagcgtt atacgcgaac gaagctgcca ttcaaccact
    21241 gcatctggat ataacgcaca ctaagagaaa tagtgctaga ttcttaacta gcactctggt
    21301 aaatacaaca tgaaaaacga aattataaac tatattaaag aagttggtcc tgtgaccatg
    21361 gaccagctgg tagatcagtt tggagccagt tctgctaaga gctttacaga cttggtcaag
    21421 ctggtttcta gcatggaagg cagtcgtcaa cttgtctttg acaatgctgg gcggattgct
    21481 ttgccagttc caaaagtgtc caaaaacaag gtgaccttac agggtatttt ccgtgcccac
    21541 aagtcaggct ttggctttgt gaccattgac gaggaggaag atgctctctt tgtaagccgt
    21601 gacgatgtta actttgctat cgaaggtgac agagttgaaa ttgcaatcaa aaaggttgca
    21661 gatcgtctca agggaactgc cgcagaagcg gaagttattg atattttgga acatagcctg
    21721 aaaactgcgg tgggcttgat tgtctttgac gaagacaagc cagaatatgc aggctacatc
    21781 aagtctaaaa accagaaaat cgctcagaag atttacatca agaagtcacc tctggtattg
    21841 accggaacgg aaatcctaaa agtcgacatt gaagcctatc caaataagaa acgggaccac
    21901 tttattgcga ccattcggga cgtggtgggc cataaggacg atgtgggaat cgatgtcttg
    21961 gaagtcttgg agtctatgga tatcgtgtcg gagttccctg atgaagtctt ggcggaggcc
    22021 aatcgggtac cagaaagtcc gtctgagaag gactttgaag gacgcttgga cctgcgggat
    22081 gaaatcatct tcaccataga cggtgcggat gctaaggact tggacgatgc ggtccatatc
    22141 aagcagctca agaacggtaa tctggaactg ggtgtccata tcgctgatgt cagctactat
    22201 gtgacggaag gctcagcctt ggatcaggaa actgttaagc gtgggacttc tgtctatgtg
    22261 accgaccgtg tggtgccaat gctaccagaa cgcctgtcaa atggcatttg ttcccttaat
    22321 ccaaatgtgg accgtctgac ccagtcagct attatggaaa ttgaccgcaa gggcaaggta
    22381 gtcaagcact ggattggcca gacggtcatc aaaaccacct tccgcatgac ctattccgat
    22441 gtcaatgata tgattgctgg taacaaggaa aagcttgcta catacaaggc aatcgtgcca
    22501 agtgttgagc tcatggtcaa actccatgag acgttggaaa ccatgcgcta taagcgtggg
    22561 gctctcaact ttgacacgac agaagctaaa atcatcgtca acaaggatgg tctgccagtc
    22621 gatattcaac tgcgccagcg aggcattgcc gagcggatga ttgagtcctt catgctggct
    22681 gccaatgagt gcgttgctga gcattttgcc aaactggacc tgcctttcat ctatcggatc
    22741 cacgaggagc ctaagtcaga caagctgcaa aagttcatcg actatgcgac cagctttggt
    22801 ctgacggtct atggtacggc cagctctatc agccaggacg cccttcaaga cctcatggag
    22861 cgggtcaagg atgaacccta tgcggatgtc ctcaacatga tgttgctgcg ttctatgcag
    22921 caggcacgtt actcagagca caaccacggc cattacggat tgggtgcgga gttctacacc
    22981 cacttcacca gtcctatccg ccgctatccc gacctgctgg ttcaccgtat ggtacgggaa
    23041 tatggtcaca atcctgtgga aaaagcagag cattttgagc aggttattcc tgagcttgcc
    23101 aagtcttcgt ccagtttaga acgccgtgcc attgaggcag agcgggaagt ggaagccatg
    23161 aagaaagcag agttcatgca agaatttgtt gggcaagagt ttgatggtgt cgtgtctagc
    23221 gtggtcaagt ttggtctttt tgtcgagtta ccaaatacgg ttgaaggtct gattcatatc
    23281 accaacctca acgaatacta ccagttccac gagcggactc tgaccttgca gggtgagaaa
    23341 tcaggtcggg tcttccgtgt tggtcagcca attcgtatca agctaacgcg ggcggacaag
    23401 atgacagggg aaatcgattt tgcccacgtg ccgtccgaac tggatattgt cgaaaaagct
    23461 ttgaaagcca agcggagaac tactagtcac tccaaccgtg accgtgatga tcggtcaggg
    23521 cgaggacggg gcaagcacta caaacaagaa gctgctggtc gcgacagcgg acgtcacaag
    23581 tctggaaaag accacaagtc aaaaagtggc aaaaaagaca agaaaaagaa accattttac
    23641 aaagaagtaa cgaaaaagaa taagaaaagg agataggcat ggccaagggt gagggcaatg
    23701 tgattgcgca aaataaaaaa gcccgccacg actatactat tgtcgataca gtggaggcgg
    23761 ggatggtcct gacggggacg gagattaagt ctattcgtgc ggggcggatc aaccttaagg
    23821 acggctttgc ccagatcaag cagggcgagg cttggttggt caatgtccat atcgctccct
    23881 acgatgaggg caatatctgg aatcaggagc caacgcggac ccgcaagctc ctcttgcgga
    23941 agaagcaaat cgagtctctg ggcaatgagg tcaagggaac agggatgacc ctggttcccc
    24001 tcaaggtcta tatcaaggat ggctttgcca aggtcttgat tgggcttgct aaagggaagc
    24061 acgactacga caagcgagag tccatcaaac gccgtgaaca agaccgtgat atcaaacgaa
    24121 ccatgaaggc tatcaatagt cgatagaggc tgggagaaat ccggtctttt ttgttatagg
    24181 aaaacaaata ccccagacag tttcgagact atctggggct tattatttat cttaatccac
    24241 cgtaatcata gctttaatcg ttttccggtt ggccatgtct ttgtaggctt ggtcgatatt
    24301 gtccaggctg taagtttggg tgaaaactcg acctgggttg atgtcgcctt tgagaacagc
    24361 atcaagcaaa atttccttat cataggttgt gacagaagca gagccaccac cgataatgat
    24421 attttgagca aaagttgatc cgagggcgtg gttgttgtaa tgaggcacac caacaaaacc
    24481 gatgcgaccg ccattatgaa ggacgccgag ggcttggtca atagaagctt ctgttccaac
    24541 acattcgagg gcagcatcag cgccacctcc aagaatttca cggactttgg caattccttc
    24601 ctcgccccgt tcagcaacaa cagcagtagc ccctgattcc agagccattt gctgacggtc
    24661 agcatgacgg ctcatcatga taatctgcga agctccccgc atttttgcag caatgacggc
    24721 acattgcccc actgcaccat caccgatgac aaccacctta tcaccaggtt tgacatctgc
    24781 aacacgctta gcatggtagc ctgttggcat aacatctgcc agagtcaaga gagatttgat
    24841 catgccttct gagtagtcgc ttggctgacc aggaattttg accagggccc agttaccgta
    24901 gtggaaacga atatattgag actggtagtt gttgcccagt tggtgtagcc tggatggttg
    24961 tcacaagtac cgtcaaagcc agcacgacag gcatcacagt gtccgcaacc atgtgtaaat
    25021 ggaacgataa cgaagtcacc aggaacaagt gttgtaatgt cttcgcctac ctcttcaacg
    25081 ataccgatgg cttcatgacc agagtttgtg tcaccgtggt catggtgaat gtcttctccg
    25141 tagccccaaa ggtccgaccc acagacacag gcccgaacga ccttgataat gacgtcgtct
    25201 ttggcttgaa ggcttggttt ttcgatttct gcaacggtca tcttaccggc tttttcataa
    25261 atagctgctt tcatattttc tacacaccta catttattaa taaatgtcct ttctgattga
    25321 tatggaatat tatacacctt ggagttcact ccaagtcaag aaaaaaatgg gtttgaattc
    25381 atatttcctt tgtgatagac taagtgtatc ggaaagaaaa ggagagataa tgacggtttt
    25441 agttccttac aaacgcatgc ccatttggaa tcaggagacg gttccccagc attttttaac
    25501 caagcacaat accaaggtgg gtacttgggc caaaatcaag gtcttgagag gtcaactcaa
    25561 atttgagccg atttcagatg ataatgagat tttgggagaa tatgtctatg gtccgacaga
    25621 cgatattccc tttgtcgagc cacaggcttg gcatcgggtg actttgctga cagaggatac
    25681 ggagtttttc ttggaatttt tctgtcaacc agaagactat tttgctaaaa agtatgacta
    25741 cacgcggacg cattcggaag tcttggaggc cttgaagaga attgagcctt gcaaggtctt
    25801 ggacctgggc tgtggtcatg gtcgtaacag tctttacttg gctcagcagg gatttgaggt
    25861 gacggcggtt gataaggatg tgccaagtat tcggacactc ttgcaggtca aggaagtgga
    25921 ggacttggac ttgcaggctg ggacctatga catcaattca gccagtctgg agcaggacta
    25981 cgattggatt atctcaacag tggtctttat gttcttggag cgggatcggg tgccagccat
    26041 tatccgcaat atgcaggagc agacacgacc tggtggctat aacttgattg tggcggccat
    26101 ggatacggaa gatgcgccct gtcctatgaa tttctccttt acttttggag caggtgagtt
    26161 gaaggaatac tatcgtgact gggaactggt caaatacaat gaagattttg ggcaactcca
    26221 taagcgggat gaaaatggga atttcctcaa gatgcggttt gcgaccatat tagctagaaa
    26281 aaatgtgtaa tatatatctg aaagcacttg caaggcaagt gtttttgcgt tacaatgtag
    26341 gtgcaaatgg gtaaatcata ttatactcca cgaaaatcac cttctgtttg ggtcggaggc
    26401 ttgtgtaaaa tagtctctgg attcctatct ttggaaggag cctacttctg attttcacag
    26461 agtatcagga aaggagaagt ctatgaaaaa gacttttgtt cgtatagcca catttctaat
    26521 gttggtttgc ctattccctg ctcaacttgt tcaagcctgt tcaggcttta tcattgggaa
    26581 agggttgaca acggatggtt ctatccttta tggtagaacg gaagattatc cctatccacc
    26641 agataatgga gcgcacaata aaaattatat tgtggttcca gcagcaacct atactgaggg
    26701 agatatgttg gtggatgaaa catttggttt tacagcacca cacttggcaa atgagttcaa
    26761 gtacacctct acgcctgatg cggcacgtgg ggatgggtca aacggtaatt ttggtgcgca
    26821 tggatttaat gaaaaaggtg tgtccatgac tgctactgtt acagctattc caaataaaaa
    26881 agttttagct gttgacccat tggtaactgc ggggggacta ggagaggcca ttttgattga
    26941 ctatgttctt cctagagtca ccagtgcgcg tgaaggtatc gaattaattg cgaagacaat
    27001 tgatgaaaaa ggttcggcag aagggaatat cattgttatt gctgataaga atgaagtttg
    27061 gtacatggaa attttgtcag gccaccaata cgtagccatc aagttccctg aagacaagta
    27121 tgcaattttt gcaaatactt actatctagg tcatgttgat tttaccgata cagaaaatgt
    27181 gattgcttct gcaaaggttg aagaagtagc caaacaggct gaaaattata tgatggttga
    27241 tgggaagttc catattgcca aatcatatgg acctgagaat tatgctgatg gtgatcgttc
    27301 tcggacatac gctggtataa aattgttaga tccagtatcg cctgtaacct atgaagatgc
    27361 tgtgtatgat ttgctacgtc agccaacaga cccaagtcgt cgctttagtt tacaagatac
    27421 ctttgcctta caacgtaacc gttttgaaca tcttccagaa tttcgtccag atgatgaagc
    27481 gggtaaggta aaacaagggg ataacggtgc gaatgatcaa gcggcagatg cgacttataa
    27541 atacgctctt ggtaatgaaa atgtcatcga tgcccatgtt tatcagatca atagtagctt
    27601 accatcagct ttcggtggca cagtatggtt gggcttggct cagacaagga atacaccgta
    27661 tgttccattt tatggtattg tgactgatac ttatgaagcc tttaaaaatc gatcagcttc
    27721 ttatgacacc aattcatggt actggacggt gcaaaatatt gataagatgg ccatctcaca
    27781 cccagagtta tttggtacat caattcaaga aaaatggatt gcacttgaaa aagaatggat
    27841 tgcttcacag gcagctcttg atgctcaata tgctggactt tcagaagatg cagccgtagc
    27901 tctggctcca actgtaaccg aggaaacctt ggctcgttca gcagagattt ttgcccaact
    27961 aaaagctgtt gaagctgaaa tgatggcaaa aattgaagca gcaacaactc cgtcatccag
    28021 cagcaccgac tcatcaagta gtactgaacc atccactagt accgaaccat catcaagtag
    28081 tactgaacca tcgaccagca ctgagccatc atcaagtggt accgaaaact caaccagtac
    28141 ttcaaccagt acttcaagca gtacaagtca gtctacaagt gcttctgaca caggaggagt
    28201 tacggatact tcctcctcaa gaggaatagt agtgtcttct gataagaaag taacacctac
    28261 caataaaaaa ggtaaatcaa gtcttcctag tacaggtgag caagtcagcc tcttgttggt
    28321 ggcgctaggt gttgcaggaa ttttaacagc aatttttctt catcggaaaa aatccagtag
    28381 agaataaatg aatatgtaaa agtaggttgg attgatttca atctactttt ttgaaacaat
    28441 tttgtttaca aatgtagtcg tatgttgtat aatttgttta cagatgtagt cggaaaagga
    28501 gtaacctatg aaacaagcaa gctatcccat tcaaggtatg acctgcgctt cctgtgctct
    28561 gacggttgag aaagcggttg gtaaactagc aggaatggaa gaagtcagtg tcaacctagc
    28621 gactgaaaaa ttgagtgtta gctatgatga aaagcttttg ggactagagg atattcgtca
    28681 ggcagttgaa aaggcgggtt atcagttagt cgataacttg gtgaccgagt cttatgacat
    28741 ttcaggcatg acctgtgctt cctgtgctct gacggttgaa aaagctctgg acaaactaga
    28801 gggtgtagaa gaagtctctg tcaatctagc aacagaaaag gcgactatcc gttatagccg
    28861 tgataggcaa aattcagcta gtcttgaaag agcagttgag caagcaggtt atcagctgat
    28921 tcgaccagaa gaagtagaag aggctgcgga caagggaccg agcaaggaag aaaagctgtg
    28981 gtatcgattt gcctggtcag cagcattcac tcttccactg ctctatattg ctatgggtcc
    29041 tatgttacct tggggagggc ttccattacc agctttgctc catcaaccgc tagtctatgc
    29101 gatcagccaa gtcatcttgc tcatccctat cctctatatc ggacgcagtt ttttccaaaa
    29161 aggctttaaa actctcttgc aaggccatcc caatatggat tccttgattg ctgttgggac
    29221 aggggcagcc ttggttcaag gtcttttgat gattgttttt cttctgatgg ggaaagaagt
    29281 ggccatgcac ggtcaccatc cagaactgta ttttgaatca gccgccgtta tcttgacctt
    29341 gattacccta ggaaaatatt ttgaagctag agctaaaggt cagacttctg aggccattaa
    29401 gaaattgatg gatctggctc ccaagacggc ccaggtcttg cgaaatggtc aggaaataca
    29461 ggtgccgatt gaagaagtgg tggtcggtga ccaggtcatt gttcgtccag ggcagcagat
    29521 tcctgttgat ggtcaggttc tcgaaggcca aacacgtgtg gatgagtcta tgctgacggg
    29581 tgagagcctg ccagtgaaga aagctctagg cgataatgtt tttggcggca cccttaacca
    29641 gcaaggcgcc attaccatgc gggctaccaa ggttggtcgt gatacgactc tggcccagat
    29701 tattcgcttg gtagaagagg ctcaaggttc taaggctccc atcgccaaat tggctgacca
    29761 agtatctgcc atatttgtac cagtcgtcat gggcttggct ctcttgtcgg gtctggcctg
    29821 gtacttcttg ggtcaagaat catggatttt ttcactaagt attatcatcg ccgtcttggt
    29881 catcgcctgc ccttgtgctc tgggcttagc aacccctacg gctatcatgg ttggtactgg
    29941 aaaaggtgca gaaaatggtc ttctgttcaa gtctggtcaa gctattgaaa ccctacaagg
    30001 tgtgaacacc attgtcttcg acaagactgg gaccattact gagggcaagc cacaagtgac
    30061 agacatccac cttttgtcta ctaaaaatcg tgagcaggtc ctccagctgg cagcaagtag
    30121 cgaacaattt tccgagcatc ccttggctca ggctctccta caagcagctt ggacagaaaa
    30181 gattgttctc ttgcccgcca ccgattttca ggcactttct ggtcgtggtc tttcggtgat
    30241 aatagcagag cagaccatct atctgggaaa tgaacggttg atgcgggaac agggaattga
    30301 tgtttccaaa ggacgtgcag ttgcggaggc atttgcccag caagccaaga cgcttgtttt
    30361 cctagccagc cagcaagaag tactagcagt cattgccatt gctgataaag tgaaagagac
    30421 cagtcgccaa gctatccagg ctttgcaagc tatgggctta gaagtagtca tgctgacagg
    30481 ggataatgaa aaaactgccc aggccattgc caaggaagtc ggcattgagc aggtggtcag
    30541 tcaggtcttg ccagatgaca aggccaacca agtcaagttc ttacaagaac agggcaagac
    30601 agttgccatg gtgggggatg gcatcaacga tgccccagct ctagctcaag cccatgtggg
    30661 tctagccatt ggttcaggga ctgatattgc cattgagtct gccgatattg ttctcatgca
    30721 tagtgatatt ttggatgtgt tcaaggctgt caaactcagc caggcaacca tgcgaaccat
    30781 caaacaaaat ctcttctggg cctttgctta caatgtgatt ggcattccca tcgccatggg
    30841 tctgttacat gtatttggtg gacccctgct caatcctatg tttgcaggtg cagccatggc
    30901 cctcagctca gtgtcagtcg tcttgaacgc tctgcgattg aaaacctaca aactatagca
    30961 aaaataccag tcaaaaaact ggtatttttt tacataaacg cttacaatag atatattaag
    31021 tgaaagggag atgtttatga caacctttat tggaaaaccg gtgaccttgg taggtccgca
    31081 gttgcgagtg ggagacacgg ctcctgactt tgttctcatg gccaatgact tgagcttgaa
    31141 aagcctgaaa gactttggca agcagaccaa ggtcatcagt gttgtgcctt ctattgatac
    31201 agggatttgt gatacccaga cccgtcgctt caatcaggaa ctggctgacc gtgataatac
    31261 agttgtcatc accgtttcag ctgacctgcc ctttgcacag aaacgctggt gcggggctgc
    31321 aggtttggaa gatgccatca ccctgtctga ttactatgac catgcctttg gtaaatccta
    31381 cggtgtgctc atgcgagagt ggaatttgct ggcgcgtgcg gtctttgtcc tcaatgctga
    31441 taatgaaatt acctacgtag aatacctgga caatgtcaac gaacatccag actatgatgc
    31501 ggctttggag gctgttaaat aaagatataa cggtgccgga acaggcatcg ttttttgatg
    31561 gaatattttt gtctaatttg atatactgaa tttatcaaaa acaaggaggg tcttttatgg
    31621 aaaagacaca gattattcaa gacgttttgg ccctcattca agacttgaat cgggcctttc
    31681 aagcagataa agtaggaaca gctgatgaac aacggttcca acataatctg acagagacga
    31741 ctcgcatatt gtcggaggcc aagtcggtca ccaatacaga actgattgcg attgagaaat
    31801 tttatcgttc gaccagtttt ttggtcggat taggcgacct gcgattgagt gagactagca
    31861 ggcaggcttg gcgggcattt gaccgatatt actaccaaac gatcagagaa gaactcaaac
    31921 tctatggggg ctctgctatt gcccaagtgt aaaagatgaa ggagagggtg aaaaccctct
    31981 tttgtgctat aataagtagt aagaatacag ttgttaggaa gagaagaagc gaggtgtgcc
    32041 atgtcctaca tccaagtaaa gaattcgtca aaatactacc aaatggggga tacaaccatt
    32101 gtggcaaatg atggtgtgtc ctttgagatt gagcagggtg agttggtcat cattcttggc
    32161 tcatctggtg cgggcaaatc cactctgctc aatatcttag gcggcatgga tagcaatgac
    32221 gagggggagg tttggattga cggtgtggat atcgccaagc tttcaagtca tgaactgacc
    32281 aattatcgta gagatgatgt aggttttgtc tttcaatttt ataacttggt tgccaatctg
    32341 actgccaagg aaaatgtgga actggcagca gaaattgtca aggatgcctt ggatgctgag
    32401 gaagtcttgg aacaggtcgg actcggtcac cgaatcaata atttccccgc tcagttgtca
    32461 ggcggtgagc aacaacgtgt ggccattgcc cgtgctgttg ccaaaaagcc taaaattttg
    32521 ctttgcgatg aaccaactgg cgctctggac taccaaacag gtaagcaagt cttgcaaatc
    32581 ctacaagata tgtcccgcaa gcagggggcg acggtgatta tcgtgaccca caatagttcc
    32641 ctagccccta ttgcagacag ggtcattcgg atgcgggacg cccgtgtgca ctctgttgag
    32701 ctgaacgcag atccgcagga tattgctagt ctagaatatt agtgggtgaa actatgaaaa
    32761 agaaaatcta ctggaaggat atgagacaat ctctgctttc ttccaagggg cgttttttgt
    32821 ccatatttag cctgatgatg ctaggagctc tagttttgac aggtctgaaa gtaacagctc
    32881 ccaatatgga aaagacggcc caagcctata tagccagtca tcgaacgatg gatttggcag
    32941 taatttcagg tctgggactt agtcaagctg accttgacga attagaaacg atagaaggtg
    33001 ctactcttga agcgggctac tttaaagatg tggttactaa tgaaggacag actgcaattc
    33061 ggttgttttc agctccccag aacctatcga cctataagct agtagaaggg gaaatgccca
    33121 gccaaaaggg gcaaatagcc ctatcagctt ctttaaaaaa tcgttacaaa ttggacgata
    33181 ctgtccaagt gacggagtct gataaggatg gaaaaatctt gacacagaca agttttactc
    33241 ttgtgggctt tgtcgcatca gcagaaattt gggacaatga aaccatgggc atggctgcaa
    33301 gtggtgatgg gcagttaggt ggctatggcc ttgttagtca ggatatcttc aaatcagaag
    33361 tttacactat tgctcgtatt cgctatgatg atttggttga tttaccatat tacagtcagg
    33421 cctatcaaga caggttggac cagcaccaag aagacttaga aaagttattg gctaacaatg
    33481 atgaacaacg attggcaact attcagtcag atggacagac agatatttca aaaggggaag
    33541 aggaaattgc tcaggctcaa tcccagcttg aacaggcaga gtcagaactg gaaacagggg
    33601 aggagcaatt agcgacaggt cgtagagagt ttgcgcgtgg tagggctaag gttgtccaaa
    33661 cggaagctga attacgagct gcccaagccc aactattaaa aaataagttt gaattggaag
    33721 aaagtaaaaa ggaattggac gggcgtaagg ctcgattaga acagactgct tcatttctag
    33781 acggctatca gatggaatta gaaacgtcag cccagcagtt agaagaggcc aagcaagaat
    33841 tggattccca aaatgcccaa ctgagtcaga ccgcatctca gatttccaca gggcgagcta
    33901 gttggtttca agctcagcaa gagttagacc ttgaaattgc caatcacttg caagaaggtc
    33961 aaaccctctc agactatcct gacttgttgg ctagacagga aggtttggat gttgagaaaa
    34021 gccgattaga ccaaatggaa tctgcacatg agcaggccaa ccaagcctat ttacaaggtt
    34081 atgattacta tcaaaccaaa caaaatgaat acaatagcaa tctagcccag taccaaactt
    34141 ggaatcaaga atatcaggca ggtttggccc gttatcaagc aggtttgagt cagtatgaac
    34201 aaggcattgc agactacaat aaaggagtag aagactatga atggggactg agtcagttag
    34261 agtcttcaaa tcaactgctc cgtcaggaag aactccgttt ggaggaggct gataaagagt
    34321 taagtcaagc tcaatcccaa ttttctgaga agaaagccac ggccgatcaa gaaattagcc
    34381 aagctcaaac cgagattgct caggctaaat ccgacttaag taagctggaa aaagcaccct
    34441 atcaggtcta tactcgatca agcttacctg gtggggatgg ctatactact tatagcaatg
    34501 cgactaggtc tattgctgca gtgggcaatg tcttcccagt tgttctctac cttgtcgcag
    34561 ctctagtgac ttttaccacc atggcccgtt ttgtcgatga agagcgaaca cagtcaggtc
    34621 tcctcaaggc ccttggctat acgaatagac agattatggc taagttcatt ctctatggtt
    34681 tggcggcagg tctggtagga acaatagtcg gtataatagc aggcaatctc ctcctgtctc
    34741 cacttatttc taacattatt acgcagacaa ccgtgattgg accagctaaa cttcattttt
    34801 atcccctttg gacaggttta gccttgctcc tgtctttggc ttcctcggtc ctgccagctt
    34861 acttggtggc tcgtcgggag ctgacagaaa aacctgctca gctactttta cctaagccac
    34921 cggttacagg ctccagcatt tggctagaaa aatggccagc tatctggtct cgcctgagct
    34981 tcacccacaa ggtgacagcc cgcaatatct tccgctacaa gcttcgtatg cttatgacca
    35041 tctttggagt agcagggacc gtcgcccttc tttttggagg actgggtatc cgttcctcta
    35101 tttcaggagt tgtccaacga cagtttgggg agctgatcca ttatgatatg ttggtggtgg
    35161 aaaatagcag agctactgag gaagagttgg ataagttaac acattttctt cagtcaaatc
    35221 aggttcgcca atccttgcca gtggcttttg aacagctcaa tcaaacggtg gatgaaaacg
    35281 gtcagagaaa gaatctatcg attagtctct acatttcaga ccgtcgggat tttggaaatc
    35341 aagttagtct ggaaagttcg acaggtcagc ctattaaact gagtgacagg gggattgttc
    35401 tcacagaaaa attggcgcaa atctacgggg tctctgttgg tgataagtta tcactgacct
    35461 tggaagataa ggaagtttca gtcagggtag aggctgtggc tgatatgtac gcagggcatt
    35521 ttatctatat gacagatagc tactacgaac aagtgaccgg caagcagaag actgccaatg
    35581 cctatctggt tcagttgaaa gacaggcagt tggggcatat tcagactctt gcaagtcaac
    35641 tcctagcgat gccagcagtc agaagtttgg ttcaaaatac ttctcttata gacatgctta
    35701 ccacgattgc gggctcgctt cagaccatca tgaccattct agtcatccta tccatcttgc
    35761 ttggcttggt tattctttac aaccttacaa ttatcaatat gtccgaacgg attcgtgaat
    35821 tatccaccat caaggtactg ggcttccaca ataaagaggt gaccatgtac atctatcgtg
    35881 aaaccattgc cctttcgctg attggtatgt tggttggact ggttagcggt atttatctcc
    35941 acaagctcct tcttgccatg attggttcgg acagtatccg cttcaatcca tctgtcggac
    36001 tagaagtcta tctcattcct atcttagcca ttagtggaat tttggcagct cttggttggt
    36061 atgtcaatca ccatctaaga aaggtggata tgctggaagc cttgaagtcg gttgattagg
    36121 gagtgggaaa gaactcgagt ggtctaaaaa gagttcgtct tcccaccccc gcacagttga
    36181 ttaggttgga tttggagtat aaaacacgaa caaatccgcc aatcaaccac tgcgctgaga
    36241 tgttgacacg aactctaaga agtggtgctg ggctttttgc ccagcctcta acccgagcta
    36301 taaaacacgg aagcgaggac agtctatata gataactaat ttgaaatatg agaaacacag
    36361 ctataaagca aaaaaatcct agtgtatcta ggattttttt gcaaacataa gtctagaaat
    36421 atgctataat catctcatga aaaaaataag tgatttagga ttaacaggtc gaaaattagt
    36481 tggagaaggt ctaatcttag tcttcatcgg tattggattt ttaattgctg gttggcaatt
    36541 tccaggactg attcttcgat ttgttcacgc aggtctattc tttttagctt tatatgaact
    36601 gagcatgata tttttcagaa agaagaaatc gagtgaaagt gtcattgcgc tagttgggaa
    36661 ggctgttctt ttcgggatcc tagccagtct cgatttagct gttcaaattc ccctctattt
    36721 cgcggctatt tttgttggga tttaccagct ttttacggca gtcatcaatt tcattacttt
    36781 ttacctatat cgaaaagatg gagtacagcc acggattcgc tatttaatag atggaatttg
    36841 gttatcctta ctaggttcag cttccctatt tgtgtcaggc acacagctgg ttgttcaaac
    36901 cattgtcatc ggaggttatc tgatcttgta tggactaact aatctgcgtg atggcttctt
    36961 gtttgaggag gctattgagc aacaaaatct taagcgccat gtccgtttgc ccctaccttt
    37021 atttttagca gctttgattc ctcgtatgac tctgcaaaaa gtcaatgatt acttggcgga
    37081 taatgaggga caaacggctc aatctatcta taaccgccac aaagaaattg cggagcttcc
    37141 agcattagaa gtctttgtac acgttggaga agaagggttc ggagcagttg gacatgttga
    37201 tttgagttac aaagggcagg tctacggttt tggttcatat gatgtcctgt cagagcgttt
    37261 gggtggtgcg attggtgacg gagttttgtt taaagctgag cgtcaggcct atattgattt
    37321 ttgtaaccaa gaaagaatga ctatgttggg ctatcaattg gcattgagtt ctgagcagga
    37381 aaaagcggta gaaactcgac tagctgaaat agaaggttta ctgcttccat ggcaaccaag
    37441 tgcggaaaag gtctctagaa gaccagacgg ccagcctatc gaaatgtatg cctaccgcat
    37501 gaaagaggca attggggcag ctcttttcaa atttaaaaaa tcaaaattca aaacctactt
    37561 tgtcctctca accaactgcg tccttttggc tgattcagta atcggtcaag caggaacaga
    37621 tgtcctaggc ttgcgtggtt tcattgctcc aggaacctac caatcttacc tagatcaaga
    37681 atatgaaaaa cctcatagtt tagtagtcgc aaagaacatt tattatcgaa aagagaaatc
    37741 ctagtcactg actgggattt tctgttattc cagccctccc cagtctacta aaacatactt
    37801 ttcatgctat aatagagaca tgaaaaaaaa taaattatta ttaatcgatg gctcttcggt
    37861 agccttccgt gctttctttg cactttataa tcaaattgat cgttttaaga gtccgacagg
    37921 tcttcacacc aatgctatct acggcttcaa tctcatgctg gaccacatga tgaagcggat
    37981 tgagccgacc cacattcttg tagcttttga tgctggaaag accactttcc gtacggagat
    38041 gtatgcagac tataaggcag gtcgtgccaa gactccagac gaattccgtg agcagtttcc
    38101 ttttatccgt cagatgttgg atgccatggg agtcaagcat tacgaactgg atcagtatga
    38161 ggcagatgac attatcggta ccttggacaa aatggcagag cggacagaga tttctttcga
    38221 tgtgaccatt gtcagcggtg ataaggactt gatccagctg acagacgaaa acaccgtagt
    38281 tgagatttct aagaaaggcg tagccgaatt cgaagaattt accccagcct acctcatgga
    38341 aaaaatgggg attaccccta ctcagtttat tgacctcaag gctctaatgg gagataagtc
    38401 cgataacatc cctggggtga ccaaaatcgg tgaaaaaaca ggcctaaaac tcctgacaga
    38461 atttggttct ttggatggca tctacgaaaa cattgacagc atgaaggctt caaagatgaa
    38521 ggaaaacctg attgcggata aggaacaagc atttctttct cgaacccttg ccaccattga
    38581 cacaaatgct ccaatcgaaa ttggactaga tgatattgtt taccaaggtc caaagctaga
    38641 agtactaggt caattctacg atgacatggg cttcaagcaa ttaagggctc agttaggtac
    38701 gaccagctcg caagaggaag cggtactcga tttccaaatc gtaacggaaa tcagccccgc
    38761 tatgctcaaa caagaccggt ttttctattt tgaaatcttg ggtgaaaatt atcaccgaga
    38821 ggatttggtt ggtctagctt ggggagataa ggagaaaatc tatgtcggag ggcctgagct
    38881 acttgatagt ccagtcttgc gtgacttttt agaaaaccaa accataaaaa cctatgactt
    38941 taagcgtgga aaagttcttc tcgaccgaaa agagattacg ctacctccag ctactttcga
    39001 tagccgtcta gccaaatact tgctttccac cgttgaggac aattccctaa caaccattgc
    39061 caacctctat ggtcagacca gtcttgttcc agatgaggca atttacggca agggtgctaa
    39121 gttagacttg ccagaacgcg agatcttttt cccacacttg gcacgtaagg ttcaggtttt
    39181 gattgagaca gaagagccga tgttgaccaa attagaagaa aatcagcaat tagatctttt
    39241 gtttgacatg gagttgccac tggcaaatgt tttagctaag atggaaattg ctggtattaa
    39301 agtggaagct gagactctca aagctatgca atctgaaaac gaagttttga ttgaccagtt
    39361 gaccaaggaa atctatgaac tagctggtca ggaatttaat atcaattcgc ctaagcagct
    39421 tgggaccatt ctctttgaag agatgggact accattggaa tataccaaga aaaccaagac
    39481 aggctattca acagcagtag atgtcttaga acgattagcg ccaattgcac cagttgtttc
    39541 aaaaatcttg gaataccgtc agattaccaa gcttcaatca acttatgtag tcggcttgca
    39601 ggatgccatt ttagaagatg gtaagatcca tacccgctat gtgcaggatt tgacacaaac
    39661 aggtcgtcta tcttcgacgg accctaactt gcaaaatatt cctgttcgtc tagaacaagg
    39721 gcgcttgatt cgtaaggcat ttgtgccatc gcttgaaaat agtgtccttt tagcatcgga
    39781 ctattcacag attgaattgc gcgtcctggc tcatatttct caggaccagc atttgattga
    39841 ggccttccag caaggtgcag atattcacac ttcgacagct atgcgggtct tcggtatcga
    39901 aaaagctgag gatgtgacac caaatgaccg tcggaatgcc aaggctgtaa acttcggtgt
    39961 cgtctatggt atttcagatt tcggtttatc caataactta gggattacac gtaaggaagc
    40021 taaagcctat atcgatacct attttgagcg cttcccagga attaagaatt acatggaaac
    40081 cattgttcgt gaggcacgtg ataaggggta tgtagagacg atttacaaac gccgtcgtga
    40141 attaccagat ataaactctc gcaatttcaa tgtccgtaat tttgcggaac gaacagccat
    40201 caactcacca attcaaggtt cggccgcaga tattctcaaa gtagccatga ttaatcttga
    40261 caaggctttg actgaagcag gtcttgcgac acgtatgtta ttgcaagtac acgatgagat
    40321 tgttttggaa gtcccagtgg cagagctaga aacggtaaaa gccatggtaa aagagaccat
    40381 ggaatcggcg attagcctgt ctgtgccatt gattgcggat gaaaatgaag gttcaacatg
    40441 gtatgaagca aagtaagaaa ggaaaaagta tgaaaaaatt aattctttgt attggtctat
    40501 gtagtcttct acttgcaggt tgcggaaaag catcgcagaa gactgagaaa acaccagctt
    40561 cttcagggga agagttttca accaccttac cgattttgca agaaaaacag gatactacga
    40621 acgaacagtt caatgtttta gcaaatgcag aggttgtagc tgttaatagt acaccacctg
    40681 ccgacgaaac acaaaaaggc cacaagattt atgcggccaa taatggtgtt gctgaaaaag
    40741 atgaaaatgg gaatatcaag gaatatttcc gttattatga tgtgccatct acttggacaa
    40801 ttaatgtaga aaatactaag gatgagacgg tcgctgcagt ctacgatgtt aaagaggggg
    40861 gcagcaatta catggttcaa ctctataata tcaatgcttt taatcagtct ccattggaag
    40921 aaggtcgcaa tatgacgccg gaggaattag aagcacggat gcttgaaaca aatcattctt
    40981 tcaatgaaaa gacggttgtc acaatcaatg gtcaggaatg gcaggttggg cgacagttat
    41041 taacagatca aaaattagct cgtctgactt tttatcggat ggaatcgaca gctgcttatg
    41101 atgactccgt ggttgttggt tcaatctatt attccttaga tccaggagat gataaaaagc
    41161 gtaccaatct taaaaaggtt atcggccaag ttaaggatgt tgtgtaccaa attgctaaaa
    41221 aataatcaag gtagacaagc tataatcagt aacgagttaa aactgacaag attgtgttat
    41281 agcacacctt attcattaag cttttattga ttctaacatt taatatacat taaaggaggt
    41341 ctctatgtct tattccttcc aaaatcctag tcaggacagt atctttgact atctgaaaaa
    41401 tgctaagacc attgcagtgg tcggtttgtc tagtcgagaa gagacagcag cctaccgcgt
    41461 ttccaagctc atgcaggaag cgggctataa aattattcca gttaatccca aggttgctgg
    41521 tgggactatt ttaggcgagt tggtctatag tagtctagca gagattgacc aacctattga
    41581 tattgtagat gttttccgca gaagtgaatt tttaccagag gtagcacagg aatttatcca
    41641 gtcgaacgcc aaagttttct gggctcaatt gggtttagaa agtcaagaag cagaaaaact
    41701 attgcgtcag gcaggtcgta acgatattgt catgaataaa tgtatcaaga ttgaatatct
    41761 tgaaatgaaa ga
//
LOCUS       DATUCG010000005        37524 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.005, whole genome shotgun sequence.
ACCESSION   DATUCG010000005 DATUCG010000000
VERSION     DATUCG010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 37524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37524
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.005"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(444..1966)
                     /locus_tag="VCA20_000204"
                     /pseudo
     CDS             complement(444..1966)
                     /locus_tag="VCA20_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003095501.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
     gene            complement(1963..2826)
                     /locus_tag="VCA20_000205"
     CDS             complement(1963..2826)
                     /locus_tag="VCA20_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase alpha helix C-terminal domain-containing
                     protein"
                     /protein_id="HEP1834077.1"
                     /translation="MTIYEARGFSNILHPFDKLEPFEYIARFKPLVVPKEADIEEYKR
                     TQAPYCLSGKVKPDNNGSYKRNNASLIYRDLIFLDYDDIETGVDLPKIVSEALSAFSY
                     IIYPTIKHTAEKQRFRLVIKPSNVMNEAVYKKTVTEVAEKIGLPYDTASLTWSQLQGL
                     PVTTGNPADYQIIVNRGYDYIVPSQPLSDYPATAPLYADNRQPKRKSMTMRVIDTLLH
                     GFGDEGGRNVALTRFVGLLLNKGVDCDVETAYELAQIANSVTNKPIPQRELDRTFESI
                     VKSEIRKRSRM"
     gene            complement(2823..3095)
                     /locus_tag="VCA20_000206"
     CDS             complement(2823..3095)
                     /locus_tag="VCA20_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEP1834078.1"
                     /translation="MFSLSKESENSLKRGILSLISGFLKEYLKPKPRITGLITPKQIK
                     DELDVDYKTLQRWERAGLKRLSPPIKDTRKVFYKIDDLLIFLGAEK"
     gene            complement(3088..3438)
                     /locus_tag="VCA20_000207"
     CDS             complement(3088..3438)
                     /locus_tag="VCA20_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003095330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834079.1"
                     /translation="MTLPPLPENYRGVLNLIKVGADNPITGAEIALILKLEERTVQSI
                     VSRLITRYGVPIIGVRHGFNRGYFIPADKAELLDGAKAFYNQVQEEQKRLSVLLNADL
                     DSYKGLLKGADMNV"
     gene            complement(3435..3797)
                     /locus_tag="VCA20_000208"
     CDS             complement(3435..3797)
                     /locus_tag="VCA20_000208"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834080.1"
                     /translation="MTTLAKASIFIAKKENYTTKQWDRIQQQKRSEEQAHRRLTALYG
                     TRVANTVIYNVTMCYYNIKRKFADTYEQACDSLGDDMLENIIYRAVNNLPSIGAERKP
                     NQSLSDFWGQFSTREVTE"
     gene            complement(4134..4757)
                     /locus_tag="VCA20_000209"
     CDS             complement(4134..4757)
                     /locus_tag="VCA20_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HEP1834081.1"
                     /translation="MNIVYMDGKKEPYTTSEIIAECAEVQHHTITRLIREHKADFEAL
                     GILGFEIHKLDTRGQPKKSYILNEQQATLLITYLKNTEPVREFKKNLVKAFFELRKEV
                     AEFRYQRALEKPKRKTLHDSIETWEQAPKHAHPTVNNLLLKGASGLNKKQLMAQRGGH
                     NGIDSLTSAELIRYQALEDMAIAMINLGMSYQDIKTMVFRQKTEVSQ"
     gene            complement(4812..4964)
                     /locus_tag="VCA20_000210"
     CDS             complement(4812..4964)
                     /locus_tag="VCA20_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834082.1"
                     /translation="MNIADFKAKYNKLIEQATDRVTYNDRRGGIGVTTDGELLRNLLK
                     ALDAEN"
     gene            complement(5174..5371)
                     /locus_tag="VCA20_000211"
     CDS             complement(5174..5371)
                     /locus_tag="VCA20_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP1834083.1"
                     /translation="MNNNFRVILAKQRKKVSDVHEATGISKSALISLYYERTKRPDIE
                     TLQKVSDYLGVTIDELLTPEE"
     gene            5537..6124
                     /locus_tag="VCA20_000212"
     CDS             5537..6124
                     /locus_tag="VCA20_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP1834084.1"
                     /translation="MLRNNLAKLMIDRGVTATQLFNDTGIARSTISKISNNNTDKISL
                     QTIDKICNYLEVSPSEFFDFWAYDVKIQCGFDNFDTLSEVKEHWKFVPEFEEPCYLLL
                     EFRRGRDRKYLLEYKFIYRYSKDPDKAFEPSHLDKVRLNGSTSQVAIFDDMPVQFKNE
                     LIDNIKQQLSETFKVWDISPTIKYIDMFIFNPKVE"
     gene            6495..7640
                     /locus_tag="VCA20_000213"
     CDS             6495..7640
                     /locus_tag="VCA20_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEP1834085.1"
                     /translation="MKITEVKKKNGTIVYRASVYLGTDQVTGKRSKTSVTGRTRKETR
                     QKAKHAQDDFISNGYTVTKVVPVRNYQELAELWLESYQLTVKPQTFIATKRMLYNHLI
                     PVFGALKLNKLSVSYIQGFINDLSSELVHYSVVHSINRRVLQYGVSLQLLPFNPARDV
                     ILPKVPKRENKAIKFIAPEDLKALMAYMEKLANKKFSYFFDYVLYSVLLATGCRFGEV
                     VALEWSDIDLENGTISITKNYSRLLKLIGTPNSKAGVRVISIDKKTINLLRLYKNRQR
                     QLFIETGARVSAVVFATPLKEYQNMATRQDSLDRRCAEIGIPRFTFHAFRHTHASLLL
                     NAGISYKELQYRLGHATLAMTMDIYGHLSKDKEKEAVSYYEKAINSL"
     gene            7736..7972
                     /locus_tag="VCA20_000214"
     CDS             7736..7972
                     /locus_tag="VCA20_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018367459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEP1834086.1"
                     /translation="MTFEEILPGLKAKKKYVRTGWGGAENYVQLFDTIEVHGQKLEAT
                     PYFLINVTGEGEGFSMWSPTPCDVLATDWVEVHD"
     gene            7965..8696
                     /locus_tag="VCA20_000215"
     CDS             7965..8696
                     /locus_tag="VCA20_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEP1834087.1"
                     /translation="MTKTALITGVSSGIGLAQAGIFLGNGWRVYGIDQASKPDLTGDF
                     HFLQLDLTGDFHFLQLDLTGDLSPVFSWCQTVDVLCNTAGILDDYRPHLDISEDELAR
                     IFAVNFFTVTRLTRPYLQQMVDRQSGIIINMCSIASSLAGGGGSAYTASKHALAGFTK
                     QLALDYAKDKVQIFGIAPGAVQTGMTQKDFEPGGLADWVADQTPIGRWTQPSEIAELT
                     FMLATGKLASMQGQIITIDGGWSLK"
     gene            8707..9312
                     /locus_tag="VCA20_000216"
     CDS             8707..9312
                     /locus_tag="VCA20_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEP1834088.1"
                     /translation="MILETNRLLLRPWSETDAADLFSQASHPDVGPAAGWPLHQSLKE
                     SREIIKTVLSQPETYALVLKETGQVIGSIGLMLGKQSGLDLPDSEAELGYWLGQGFWG
                     QGLVPEASQVLLDYGFFQLNLEKVWCRAFVENSKSLRVQEKLGFVYQYLLEDVHFPLI
                     DEVRTERVSLLTKEEWQSRKDPYDPDTTLARISCHQSPSWF"
     gene            9254..9724
                     /locus_tag="VCA20_000217"
     CDS             9254..9724
                     /locus_tag="VCA20_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEP1834089.1"
                     /translation="MTPTQLWQEFLAINPQAGSEPEAWVFGAEAERLADLVARGIKTS
                     TSSAHALYAVEGEEIPRAGGYDIILDGQGQAVCIIQTTKVYVTPFSKVTEEHAYKEGE
                     FRQGGDLSDRKAKSLIHWRQVHEELFTIWLEEAGLSFSEDMLVVCEEFELVYPK"
     gene            9900..11282
                     /gene="glmU"
                     /locus_tag="VCA20_000218"
     CDS             9900..11282
                     /gene="glmU"
                     /locus_tag="VCA20_000218"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEP1834090.1"
                     /translation="MSNNYAIILAAGKGTRMKSDLPKVLHKVAGITMLEHVKRAVDAM
                     EPAKTVTIVGHKAELVQAVLEGQSEFALQSEQLGTGHAVMMAEPALAGLEGQTLVIAG
                     DTPLITGESLKNLINFHVSHKNVATILTAQADNPFGYGRIIRNADGEVQKIVEQKDAN
                     DFEKQVKEINTGTYLFDNKRLFEALKDINTDNAQGEYYLTDVISIFRQAGEKVGAYVL
                     RDFDESLGVNDRVALATAEAVMRKRINEKHMVNGVTFINPDATYIDIDVEIGAEAVIE
                     ANVVLKGQTVIGERTVLTNGTRVRDAKIAADAVISNSDIEESVIEEGVTVGPYAHIRP
                     GSLLKKDVHVGNFVEIKASTLGQGTKSGHLTYLGNATIGNNVNVGAGTITVNYDGKNK
                     FKTTVGDNAFVGSNSTIIAPVTIGDNALLAAGSVITKDIPEDAIGIGRGRQENKEGYA
                     TRFPFHPSQK"
     gene            11291..11836
                     /locus_tag="VCA20_000219"
     CDS             11291..11836
                     /locus_tag="VCA20_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEP1834091.1"
                     /translation="MNFEEKTIERTEIFKGHIFDVVVDDVALPNGGTGKRELIFHKGA
                     VCVLAVTPEGKMILVKQYRKAIERTIYEIPAGKLELGEEDTLEDAALRELEEETGYTS
                     DKLTLLADFYSAIGFCNERIRLYLADNLIKVENPRPMDEDEVIELHEVTLEEALNLVA
                     TGDICDAKTIMAIQYLQLMRK"
     gene            11847..12167
                     /locus_tag="VCA20_000220"
     CDS             11847..12167
                     /locus_tag="VCA20_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834092.1"
                     /translation="MGKPLLTDEILEKAKKKQYSDQSSQFGHYRPNQDISMDDALFDP
                     YLEEDYQGYEEGQTIRIPVKPTVVKSRRIETIKREAFRSKVNKILFWVIILLIIFIIA
                     VLFI"
     gene            12176..12865
                     /locus_tag="VCA20_000221"
     CDS             12176..12865
                     /locus_tag="VCA20_000221"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEP1834093.1"
                     /translation="MKFGIIAAMPQELKILVEHLQDATEIDVLGRTYYQGRIGQHEVV
                     LVQSGIGKVMSAMSVAVLVDRFSVNVIVNTGSAGAVADGIAIGDVVVANQLAYHDVDV
                     TAFGYAYGQMAGQELYYPADQVLLEQLRTVLAEQEMISHVGLIVTGDSFIAGQERIAT
                     IKTHFPEVLAVEMEGAAIAQAAVNTGKPFLVIRAMSDTAQGDANITFDEFIIQAGERS
                     AQTLIAFLEKN"
     gene            13062..13910
                     /locus_tag="VCA20_000222"
     CDS             13062..13910
                     /locus_tag="VCA20_000222"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HEP1834094.1"
                     /translation="MVQRLLLANDLPGVGKVALATSIPIAAACQVETILLPTVLLSSH
                     TGGFPDVVVEDMTDLNRAYFEQWKNLNLELAGILSGYCRNPQQLSQLAMYAKQTETPL
                     IVDPVMGDGGKLYTGFTSTYVEAMKELIQSAKLILPNVTEAAFLTGRDYLGACYDRAA
                     IEDLLEALAEQVSADIVLTGISFDDSQIGVAYFQQETGEITTYMSKKYPANFFGTGDI
                     LSTLLAVATIQQINLHQAIPLALDFIDKSLERTLALDRDLKFGISFEPFLAELQQAFQ
                     TLKEKL"
     gene            13907..14437
                     /locus_tag="VCA20_000223"
     CDS             13907..14437
                     /locus_tag="VCA20_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEP1834095.1"
                     /translation="MKQSQLRQLTEISLFAALICVVVFFIRIPFGSQFVHLGNALVVV
                     GVLIFGAKKGALAASLGLGIFDLLSGYASVAWITILESLIVCFVLHLFYEKFLQANDS
                     LSNIMLVGIVAALTKIILNLLKYTLTGSLVGGLTPLASFLAALGRILGTYGTAITTII
                     AVPILYKVCKRLYGRK"
     gene            complement(15000..15803)
                     /locus_tag="VCA20_000224"
     CDS             complement(15000..15803)
                     /locus_tag="VCA20_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEP1834096.1"
                     /translation="MKKILALALASSILLTACTNNSTANRSSSTASSSSPSSTTTADT
                     TASSQYAKDLAYAINNPKATFPQLSNEIGENEAAVKIKTTEGDITIKLFPEQAPLTVE
                     NFLTHAKEGYYNGTIFHRVIKDFMIQGGDPLGNGTGGESIWAGKDTTIDAGNGFKDEI
                     SAFLYNIRGSLSMANAGAGTNGSQFFINQNTTDMSSQLSSSSYPGKIIDAYKNGGNPN
                     LDGKHTVFGQVIEGMDIVDKIASVETDNNDKPKTDVKIESIEILKDYVK"
     gene            15946..16299
                     /locus_tag="VCA20_000225"
     CDS             15946..16299
                     /locus_tag="VCA20_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HEP1834097.1"
                     /translation="MFENLFAPDGSLVWHMALFYSFSAIILATVCFHIIKNFMEWHRN
                     NREPKESCKAKLVSKRTHVFGNEVARTNYYVTFEWEGKRQEFRLRSDEYALLAEGDVG
                     TLHFQGTRFLGFERL"
     gene            16384..16761
                     /locus_tag="VCA20_000226"
     CDS             16384..16761
                     /locus_tag="VCA20_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEP1834098.1"
                     /translation="MIQSIEQVMLYVNDIEASAQFWKEKMGFERVEKQVQGPQTSYII
                     APKSDSEVQFVLHDKAEVAEMEPELNLGIPSILMTSVDVAKTYEELVERGVTTNPVMD
                     LGFMKVISFADNDGNYYAIREVK"
     gene            16762..17307
                     /locus_tag="VCA20_000227"
     CDS             16762..17307
                     /locus_tag="VCA20_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834099.1"
                     /translation="MSLQNRVEEMYKDHEVKPYISPERDLAAWLLEAKPVPKRNMVRL
                     EEGILPGDIILLWRISLGSFETTTPYSKYFEYMYGINGPAHMEQLIADGYVYVESAFD
                     SLDHITSTAKKNILKAEGVTGLSKMKAADLDTALKDNLTEEKLAPYFTVRGYALTEKG
                     RAALDNHTEVLAKHPMKKMYK"
     gene            17464..18453
                     /locus_tag="VCA20_000228"
     CDS             17464..18453
                     /locus_tag="VCA20_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEP1834100.1"
                     /translation="MEEQKSITMKDVARVAGVSVGTVSRVINNEPGIKESTLEKVNAA
                     IKELNYIPDVYARGMKKNKTETIALIIPTVWHPFFGEFAYHVEVELSKKNYKLLLCNI
                     SGPKRELDYLTMLQQNKVDGIIAITYSPIDDYLSSNIPFVSIDRTYENKDIACVSSDN
                     QAGAELAADILIEKGGSQFAFIGGHNKTINETKKRRLYFEKRILEAGFPCQVLDLEEP
                     YDDFVGTVEKFLIANPQIDAIFTINDFVALDTLSILEKLGRRVPEDVQIIGYDGIQLA
                     GERSLELSTIRQPLEAMAQEAVACLVDIIEKRERPLQVTLPISYLEGKTTKNF"
     gene            18611..19531
                     /locus_tag="VCA20_000229"
     CDS             18611..19531
                     /locus_tag="VCA20_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEP1834101.1"
                     /translation="MKTSKLQQASLVDRIKQQKLLLLMLLPGLVLTFIFRYIPMYGVL
                     IAFKDYNPLKGVLGSEWIGFEEFTKFLSSPNFGNLLANTLKLSVYGLLLGFLPPIILA
                     IMLNQLLSDKAKKRIQLVLYAPNFISVVVIVGMIFLFFSVGGPVNSILGMFGLEANFL
                     TNPDYFRPLYILSGIWQGMGWASTLYTATLVNVDPALIEAAKLDGANIFQRIWHIDLP
                     ALKPVMVIQFILAAGGIMNVGYEKAFLMQTSLNLTSSEIISTYVYKIGLVSGDYSYST
                     AVGLFNAFINIILLIAVNKIVQRINDGQGL"
     gene            19541..20428
                     /locus_tag="VCA20_000230"
     CDS             19541..20428
                     /locus_tag="VCA20_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEP1834102.1"
                     /translation="MNTQMYTKFDKRMLIVNKILLAFLVLITVVPMVYILVASFMDPQ
                     ALVSKGISFDPKDWTVEGYQRVFSDKSILRGFFNSLFYSFSFAFVTVAISVMTAYPLS
                     KKDLVGKKWINAFLVFTMFFGGGLVPTYLLVKDLGMLNTVWAIIIPGAVNVWNIILAR
                     TYFQGLPDELVEAAVMDGANEFQIFWKIMIPLAKPIMFVLFLYAFVGQWNSYFDAMIY
                     IKDPDLEPLQLVLRKILIQSEPAKDMIGAQAAMNEMKRIAEMIKYATIVISSLPLIVM
                     YPFFQKYFDKGIMAGSLKG"
     gene            20580..22190
                     /locus_tag="VCA20_000231"
     CDS             20580..22190
                     /locus_tag="VCA20_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEP1834103.1"
                     /translation="MKHTFGKTVVTLLASTVLLAACGSKNTASSPDYELKDVQFPLEK
                     SVTLKFMTASSPLAPADPNEKLIFKRLEEETGVHIDWTNYQSDFGEKRNLDIASGDLP
                     DAIHNDGASDVELMNWAKQGVIVPVEDLIDKHMPNLKKILDENPEYRSMITAPDGHIY
                     SFPWIEELGEGKESIHTVNDIAWINKEWLNKLGLEMPKTTDDLIKVLEAFKTQDPNGN
                     GKADEIPMSFVNGNGGEDFKILFGAFGVGDNDDHIVVNNDGTVDFTADNEDYKEGVGF
                     MRTLQEKGLLDPEAFEQDWNTYISKGSENLYGVYFTWDKANITGMNDTYDILPVLAGP
                     DGTKHITRTNGMGFQRDRMVITSANKNLELTAKWIDAQYEPIQSVQNNWGTYGDETQQ
                     NIFELDTATNSLKHLPLNGTAPGELRQKTEVGGPLAILDEYYGTVTTMPDDAKWRLDL
                     MHATYLDYVTNDDIYPRVFMEQEDLDKIAQVEADMNDYIYRKRAEWIVNGGVEEEWDS
                     YLKELESYGLSDWLAIKQKYYDQYKENQ"
     gene            22208..23566
                     /locus_tag="VCA20_000232"
     CDS             22208..23566
                     /locus_tag="VCA20_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEP1834104.1"
                     /translation="MKTVVEANQFIQTEKGNVNPIFKPQAHLTPETGWINDPNGFIYF
                     RGEYHLFYQFNPYESVWGPMHWGHAKSKDLVNWEHLPVALAPDKDYDKDGCFSGSAIV
                     KDDVLWLMYTGHINNEDGTVSQVQNMAFSTDGIHFEKIEQNPVATADGLPEEVIANDF
                     RDPKIFEKDGHYYSVVATKHKDNVGCIVLLSSPNLTDWKFESIFLKGEANQGFVWECP
                     DYFEVDGQEYLIISPMRYQKDGNDFININSNIFVTGHVDWDKKVFVAESFKEIDHGHD
                     FYAAQTTEGPEGERVMIAWMHTWGRPLVTNDLGHKWYGQMTLPRLLKQGENGLRQVLP
                     AGILNSFDDIEIGQVIQGPSKLSLKLDDSLDLKLGTDRDYLQFGYDKAKQEVYIDRSS
                     LKIQQVGEEEWSTVRRAVTVQATELDVLVDTNCLEIFINDGQETLTSTFYVEGQIILN
                     AL"
     gene            23692..26034
                     /locus_tag="VCA20_000233"
     CDS             23692..26034
                     /locus_tag="VCA20_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEP1834105.1"
                     /translation="MRKTSKKGKSTRRPTKAELAQQERVKNITLRVLGLLFIAFAASR
                     LGVFGVTSYNIFRLLFGSLAYLLLAGAFIYLLIPKVLREREGTISGFWLIVIGLLIEF
                     QAYLDWTYQGSDLFGHTLKLALSDLAKFQVTAFLGGGMIGSIFYLPVSFLFANVGSFF
                     IGLLVIAFGIFFVSPWSVYDVADGLAVAKDKLVETQVKRAELRTQKKAEREEKRQQEL
                     ARLEEESARALALVDRHVDLETGEILEEEPVQVPIVSEYDHLDIEDEFPIMLAEDDQP
                     AQSNSASQASEPNEFEKDETDVQIDFKPKQRLAYKLPSIDLFAPIKAKSQSNEKRIVR
                     QNIKVLEDTFASFGIKVVVERAEIGPSVTKYEVKPAVGVRVNRISNLADDLALALAAK
                     DVRIEAPIPGKSLVGIEVPNSEVATVPFRELWEQSKTDPDKLLEIPLGKAVNGSVRSF
                     NLARMPHLLVAGSTGSGKSVAVNGIISSILMKAGPDQVKFMMIDPKMVELSVYNDIPH
                     LLIPVVTNPRKAARALQKVVDEMEKRYELFSQIGVRNLEGYNAKVEEFNSRSEEKQIP
                     LPLIVVIVDELADLMMVASKEVEDAIIRLGQKARAAGIHMILATQRPSVDVISGLIKA
                     NVPSRIAFAVSSGTDSRTILDENGAEKLLGRGDMLFKPIDENHPVRLQGSFISDDDVE
                     AIVGFIKDQADADYDESFDPGEVAEGDGDTGFGDAGGDPLFNEARALVVETQKASASM
                     IQRRLSVGFNRATRLMEELEAAGVIGPAEGTKPRKVLETQ"
     gene            26034..26420
                     /locus_tag="VCA20_000234"
     CDS             26034..26420
                     /locus_tag="VCA20_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEP1834106.1"
                     /translation="MKFDAVHHIAIIGSDYDKTREFYVEKLGFEQVDEHIRPEKNDIL
                     FNVKKGNLILEIFIKPDAPMRPAMPNPEHTGLRHLAFQVADVEACLEEFDRLDIRHEV
                     LRTDDFDGKKMAFFFDPDGLPLEIHE"
     gene            complement(26789..27157)
                     /locus_tag="VCA20_000235"
     CDS             complement(26789..27157)
                     /locus_tag="VCA20_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEP1834107.1"
                     /translation="MFESIIFLKIVAFLFIFLTLAISIIAVKFFRLQNRGWNFADIAF
                     PLYAIEFYLISDKAYYNSLLPQLVLALSLLAIGLCGFFLLKKKNFLYRRFFKVFWRAS
                     FILTFLMYLALVIAVFTLKS"
     gene            27312..27737
                     /gene="rplK"
                     /locus_tag="VCA20_000236"
     CDS             27312..27737
                     /gene="rplK"
                     /locus_tag="VCA20_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEP1834108.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFTFITKTPPAAVLLKKAAGVEKGSGTPNKTKVATVTR
                     AQVQEIAETKMPDLNAASLEAAMRMIEGTARSMGFTVTD"
     gene            27778..28467
                     /gene="rplA"
                     /locus_tag="VCA20_000237"
     CDS             27778..28467
                     /gene="rplA"
                     /locus_tag="VCA20_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEP1834109.1"
                     /translation="MAKKSKNLRAALEKIDSTKLYSVEEAVALAKETNFAKFDASVEV
                     AYNLNIDVRKADQQIRGAMVLPNGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINGGWLDFDVVIATPDMMAVVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADKAGIVQALIGKVSFDADKLVENFKAFHDVMAKAKPATAKGTYMTSVSITTTQ
                     GVGIKVDPNSL"
     gene            28562..28849
                     /locus_tag="VCA20_000238"
     CDS             28562..28849
                     /locus_tag="VCA20_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834110.1"
                     /translation="MKATCEEFYIHLSAVYQLPEDAITSVLREKVFETAKELAQADNL
                     YLLAERLGHYVNAELTALTCLTPKELVQLSLYIQQLQNHYRIASFIPGKVE"
     gene            28855..29643
                     /gene="yghU"
                     /locus_tag="VCA20_000239"
     CDS             28855..29643
                     /gene="yghU"
                     /locus_tag="VCA20_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HEP1834111.1"
                     /translation="MTEYTKPLVWKWEDENGNRSGNRPTAGSRFEQDLPKGEKSFQVY
                     SLGTPNGIKVAIMLEELKELGISEADYDLFLINIGQGDQFGSDFVAINPNSKIPAMVD
                     YSEQEPISVFESANILLYLAEKFEAFLPKAWAERAEVLNWLFWQTGAAPFVGGGFGHF
                     FHYAPTAQEYPINRYTMETKRQLDLLDQLLATREYIAGNTYTIADIAIWSWYGRLVQG
                     ELYPGSAEFLDVDSYKHLKTWVEKIAQRPAVQRGLTVKYHSIDN"
     gene            29898..31538
                     /locus_tag="VCA20_000240"
     CDS             29898..31538
                     /locus_tag="VCA20_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEP1834112.1"
                     /translation="MSEKVEMQQSKEQATMARGLAWLTAGNILSRLLGVAYVIPWYIW
                     LGEYRAEANALFSMGYQIYANFLLISTAGLPTAIAKQVAKYNVLGKEEVSLYLVREFF
                     KLMLVFGAVFAGVMYLSAPWLADASGSKEKLLPVMYSLVPPLFIFPAMSILRGFFQGR
                     HDMKPYAFSQLAEQLVRVIWILAATFMIMKLGSGDYLEAVVQSTFAAFVGTIASVGIL
                     VYTLWKQGYLGKLLHAKKQKISLDTGQLIKETVRDAIPIIILGLTIQLLQFIDQVSFI
                     RVMERITDYSNSELLELYSYMAANPSKITMMIIGISLSLGSVAIPLITEKFVKKDLKA
                     TSHLVADNLQLLFIFTIPAIVGTVLLAGPLYTIFYGPSEPIAITLFIWNLFLILPLGL
                     YSVISVVIQAIFENRRAIYYFLIGMLVKIVLQVPMIYVFKAYGSFISTIFGLGIMLYL
                     FYRRIDKVLHIDERLVIKDIATISWISIIMGLVVWGIEFVLNLILPANGYVSSFIHLA
                     VAGGAGMLVFVILTLKTRQLDRLIGSRAQSLRRNLRLG"
     gene            31705..32439
                     /locus_tag="VCA20_000241"
     CDS             31705..32439
                     /locus_tag="VCA20_000241"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEP1834113.1"
                     /translation="MMKPKYERILIKLSGEALAGERGVGIDLKTVQEMAKEIQEVAES
                     GIQIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLKQLGVDTRVQ
                     TAIAMQSVAEPYIRGRALRHLEKGRIVIFGAGIGSPYFSTDTTAALRAAEIEADAILM
                     AKNGVDGVYNADPKKDANAVKFNELTHREVISRGLKIMDATASTLSMDNDIDLVVFNM
                     NEPGNIKRVVFGEPIGTTVSNSSEEK"
     gene            32574..33131
                     /gene="frr"
                     /locus_tag="VCA20_000242"
     CDS             32574..33131
                     /gene="frr"
                     /locus_tag="VCA20_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEP1834114.1"
                     /translation="MSKEIIAKAQERMNQSHQSLAREFSHIRAGRANASLLDRISVEY
                     YGSPTPLNQLAGITVPEARVLLITPFDKSILKDIERALNASDLGLTPQSDGTVIRLVI
                     PALTEETRKNLAKDVKKVGENSKVAIRNIRRDAMDEAKKAEKAKEITEDELKTLEKDI
                     QKVTDDAIKTIDKMTADKEKELLEV"
     gene            33263..34117
                     /gene="cvfB"
                     /locus_tag="VCA20_000243"
     CDS             33263..34117
                     /gene="cvfB"
                     /locus_tag="VCA20_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HEP1834115.1"
                     /translation="MNDLLAQYIVGLVTDENDQFYFVQKEGRTFALSKDEGEYSLGQS
                     VKGFAYTDMKQKLRLTTKEVGASRTSFGWGTVTEVRKDLGVFVDTGLPDKQVVVSLDI
                     LPEIKELWPKKGEQLYVKLDVDKKDRIWALPAFQEDFQKMAGPAYDNMQNQNLRAIVY
                     RLKMNGTFVYLPDNNMLGFIHPSERFAEPRLGQVLEARVIGYRAVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSAPEDIKATFGISKGQFKKALGGLMKAGKIKQ
                     DSFGTELV"
     gene            34127..35047
                     /locus_tag="VCA20_000244"
     CDS             34127..35047
                     /locus_tag="VCA20_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000964166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEP1834116.1"
                     /translation="MLVYIAGSGAMGCRFGYQLSKTNHEVILLDNWEEHIEAIRENGL
                     KVTGDVEETVHLPIMKPTEATREADFIILLTKADQLPKMLRDIKPIIGPTTKVLSLLN
                     GLGHATTMRRYVPDESIMVGVTMWLAGLKGPGHAHLEGPGSISVQHILGDEQSVADII
                     EMLNEAGLNVTYDNHVVSAIWQKACVNGVMNPTCTILDCTIGELFGTEAGLNLVQGIF
                     KEFIAVAKSEADGIDEEAIWKYVIDASKKASNHYPSMHQDLVQHGRKTEIDYLNGAVV
                     FKGKRAGIPTPYCQMITDMIHAKESMLGLR"
     gene            35057..35512
                     /locus_tag="VCA20_000245"
     CDS             35057..35512
                     /locus_tag="VCA20_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEP1834117.1"
                     /translation="MLIEVETIEEYIAALPENRKEPVERLHQVIVEQLPAGFEVGILG
                     GMINYYVPLAAYPDGYHCTPREPVPFLALASQKAHIALYHMGIYMDQELNDWFVAAYQ
                     AQVPTKLDMGKSCVRMKNPKNIPYELIGELVSKMSMERYIELYEKNHRK"
     gene            35518..35733
                     /locus_tag="VCA20_000246"
     CDS             35518..35733
                     /locus_tag="VCA20_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEP1834118.1"
                     /translation="MRRSFYSWLMTQRNPKSNEPVAILADYAFDESDFPKQSDNFDEV
                     SRFLEESASFAFSMSDFDVIWEDYLGH"
     gene            35812..36834
                     /locus_tag="VCA20_000247"
     CDS             35812..36834
                     /locus_tag="VCA20_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003072554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEP1834119.1"
                     /translation="MLEHSIDIQLKHPDDSFSLFGSNERHLRLMEQELGVVIHARTEK
                     VQVIGQEEHVEQARLVIQSLLVLVSRGLVINTPDVVTAISMAKNDEIEKFVALYEEEI
                     IKDNYGKPIRVKTLGQKLYVDSVKRHDIVFGIGPAGTGKTFLAVTLAVTALKRGQVKR
                     IVLTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKEQTTRLMEREIIEIAP
                     LAYMRGRTLEDAFVILDEAQNTTIMQMQMFLTRLGFNSKMIVNGDISQIDLPRKVKSG
                     LIDATEKLSKIPQIDFVHFSAKDVVRHPVVAQIINAYEEEAWAADSQASFETIGEPSR
                     SEENED"
     gene            36824..36961
                     /locus_tag="VCA20_000248"
     CDS             36824..36961
                     /locus_tag="VCA20_000248"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834120.1"
                     /translation="MKISQFSSHYQVRKLADADLEQVLALYQTNPLYFEYFHPCLVWK
                     V"
     gene            36940..37326
                     /locus_tag="VCA20_000249"
     CDS             36940..37326
                     /locus_tag="VCA20_000249"
                     /inference="COORDINATES: protein motif:HMM:NF025054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEP1834121.1"
                     /translation="MPSLESLANDLVECPPGKELSDKYFLGFWDKERLVAILDPIDGY
                     PTAEIAYIGLFMVDSRLAGQGLGSKVIAKLLTQLATHFKKVRLGYVESNPQASHFWSK
                     VGLCSTGEVKEIAGKTIVIAEHALDE"
BASE COUNT        10909 a         7181 c         8255 g        11179 t
ORIGIN      
        1 aatatcgcta ggctcaatag gtgtatcatc agccatataa tttaggcggt gttgctcaag
       61 ttctaatctg tctttttccg cctgttctaa gtaatcattt tctttcattt tcagtctctc
      121 tttcttgttt taagataaat tatgtaaact tttgccattt gtgaccaact ttaaaaaatc
      181 ttggtcacgt taaaaaagtt agtagtatca agggtttagc tatggtgttg accttgtgac
      241 cgattttgaa aaaaattatt tatataaaaa agatgaaatg atttccaaac tcctatatat
      301 ataaataaaa aacttggtca catggtcaca ttttgataat ttagaggtaa aaagtcagta
      361 ttatcaaggg tttcagcgtg tgaccgtttt ttaaaaactt ggtcacatct tggtcacacg
      421 gtcacaaact ttgatacttc ttattattct tggttatctc tgtgtactcc cttaggtact
      481 ccttgaagtt ttcggctatg ccaaccgtta gggtcaagga tattataatc gtctaatggc
      541 acgcgcccag tttttaactt ataaatatca gcaacttctt tatttagtac agcagttatt
      601 tttttccaaa gctgtatagg ttagcgtttt ttatcccctc atcttctgta aattcttcta
      661 gccattgctt gataatgggt agcggtacat gttgcaactc atggtagcca ttgggaatat
      721 aagttccaac cacaaaagaa taataaaagt cgttatcctc tttgtactcg tctaacatgt
      781 ctttaaccgc tttaggttca ataaatttat caaactccgc catatttaag atttcataga
      841 gtacccactc taaaagaact ttgtttttaa taaagtcgtc tttgatttct gggcgctcta
      901 ctgtgccatt aaaatctgca ttaaatggca cgatacacaa gcgcctatac caaccttgtg
      961 acttgttgcg acttctaggg ataccatttc cagaaaagat acataaaagg cgataagtag
     1021 cctcataggg ctgtaaatgt ttagggttta cttgtatagt atcgcctgta acaatactca
     1081 ttaggtcaga aacactatca aggtatttat cagaaatatc atcaccaata ttacaaactt
     1141 ttccatttag ggctgaaagt aggtgctgtt cttgaaattg gtctggttta aggttgctaa
     1201 tgttttcctt acctatcaga ttttctaaca gggcttgaaa tgtccctttt ccgttgttcc
     1261 catctccagt taaaatgacc atctttttgc gcgtgcggtt ggggttgata gcctcgttca
     1321 tgacttgcca aagtagtgta atgatttcct tatcgtcaca agcaagagta cctagccact
     1381 tatcaaaatc aaaccagcca ccaagtaaag gcttttgagc ctctgggttg tatgctgtgg
     1441 ttatcttggt tgtgataata tgcttagggt taaatggctc taattgttta gtatgtaagt
     1501 taaaaatgcc gtttctgact ggtattaaat agctttcctg catagggggc ttgatttttg
     1561 tttctgtcct tagaaaaata atcacttcct cggtaaatcg cttagatgtc agcctgctat
     1621 caaattttag aattagctta ttgactatat cacggctagc aatataatag cctaaatcta
     1681 aatggtagat gtatagctgg ctactgtctg ttagtttact gtacccaata aaggtaaaat
     1741 agcaattttt gagtaaaata ttagctacct cagcaacact agggcgtggt attttggttt
     1801 ctacctcgcc ttttctgtta cctttagtga tttccttttc ttctttatgc tcatcacgcc
     1861 aagtctcgcc taatgattgt aaaagttggt acagctcttt catagtttgg ggctttttaa
     1921 cttgtttggc gttatctaat tcctgttgta attccttgat attcacattc tcgacctctt
     1981 tctaatttca gattttacga tactctcaaa agttctatct aattcccttt gtgggatcgg
     2041 cttattagtt acgctgttag ctatttgtgc aagctcatag gctgtttcta cgtcacaatc
     2101 aaccccttta ttaagcagta accccacaaa gcgcgtgagt gctacgttac gccctccctc
     2161 atcaccaaaa ccatgtaata aggtatctat cacgcgcata gtcatagatt ttcttttagg
     2221 ttgtcggtta tctgcataga gtggagcagt agcgggataa tcagataatg gctgactagg
     2281 tacgatataa tcataccccc tgttcacaat tatttgataa tctgctgggt ttcctgttgt
     2341 cactggtaag ccttgtaatt gtgaccatgt aaggcttgct gtatcgtaag gtaagcctat
     2401 tttttcggct acttccgtta cagttttctt atagactgcc tcattcatca cattactagg
     2461 cttaattaca agcctaaaac gctgtttttc ggctgtgtgc ttaatagttg ggtaaatgat
     2521 ataggaaaag gcgctaagtg cctcagaaac gattttaggg aggtctacgc ctgtttctat
     2581 atcgtcatag tctaagaaaa tcaaatcacg atagataagg ctagcgttat tacgcttata
     2641 acttccattg ttatctggtt tcacttttcc gctcaagcag tagggggctt gggttcgttt
     2701 gtactcttct atatcagcct ctttaggaac tactaaaggc ttgaaacggg cgatatattc
     2761 aaaaggttct aacttatcga aagggtgtaa gatattgctg aaccctctag cctcgtatat
     2821 tgtcatttct ccgctcctaa aaatattaaa aggtcatcaa ttttataaaa gactttgcgc
     2881 gtgtccttga taggagggga tagccttttg agacctgcac gctcccaacg ttgcaaagtt
     2941 ttataatcta cgtccagttc atcttttatc tgtttgggag tgattaagcc tgttatccgt
     3001 ggtttgggtt ttagatattc ttttagaaac ccagaaatta gtgataatat gccacgtttt
     3061 agactgtttt cactctcttt gcttaaacta aacattcata tcagcccctt tcagtaaccc
     3121 cttgtagctg tcaaggtcag catttaacaa aacacttagg cgcttttgtt cttcttgtac
     3181 ttgattgtaa aaggctttag cgccatctaa tagctccgcc ttatctgctg ggataaagta
     3241 accacgatta aagccatgcc ttacgccaat gataggaaca ccgtagcgcg tgattaaacg
     3301 gctaacgata ctttgtactg tcctttcctc taacttcaaa ataagggcta tctctgcccc
     3361 tgtgatgggg ttgtcagctc ctaccttgat aagattaagc acgcccctat aattctctgg
     3421 tagcggtggt agtgtcattc tgttacctcc cttgtgctga actgtcccca aaagtctgat
     3481 agagattgat taggttttcg ttctgcccca atggagggta ggttatttac tgctctgtaa
     3541 ataatgttct ctaacatgtc atcacctaag ctatcacaag cctgttcata ggtatctgca
     3601 aacttgcgct taatgttgta atagcacata gtcacattgt aaatgactgt gttagctacc
     3661 ctagtgccat atagagccgt taacctcctg tgggcttgtt cctcacttcg tttttgctgt
     3721 tgtattctat cccattgctt ggtggtataa ttttctttct tggctataaa aatgctagcc
     3781 tttgctagtg ttgtcaattt gttatcctcg ttttcttaat tttttgttaa aaggtcgttt
     3841 tcaaattggt ttcttgtgtt atgctctttt taagagttag ttctatccgt atggtcaaga
     3901 tacttaaatc atgatataat ctaagtatca gtaaattact aaaatccctt taataacggc
     3961 ttgcctgctg ttattggatt tagtttaatt gtgtgaaaag gcttggaact ttggtcggtt
     4021 gcataagcct tttttgttgc tctcacgcgc ctgctaggtg cgtttttaat catcttctgg
     4081 gctatctgtt gtaaacgccc atagtaaaag gtgtagtagt gctgttagta tgcctattgt
     4141 gatacctccg ttttctgtct gaataccatt gtttttatat cttggtaact catgcctagg
     4201 ttaatcatag cgatagccat atcttctagg gcttggtatc tgataagctc agcacttgtt
     4261 aagctgtcaa tgccgttatg tccgccacgt tgtgccataa gttgcttttt attcaatccg
     4321 ctagcgcctt ttagtagcaa gttgttaacg gtcgggtgtg cgtgttttgg ggcttgttcc
     4381 catgtctcta tgctatcgtg caaggttttg cgctttggtt tttctaatgc cctctgataa
     4441 cgaaactccg ccacctcttt acgtaactca aagaatgctt taactaggtt ctttttgaac
     4501 tctcttacgg gttcggtatt tttgagatag gttatcagca aagtagcctg ttgctcattc
     4561 aaaatatagg attttttagg ctgtcctcta gtgtctaatt tatggatttc aaatccaagt
     4621 attcctaaag cctcaaaatc tgctttatgc tctctaatta agcgcgtgat ggtgtggtgt
     4681 tgtacctctg cgcattctgc gataatctcg ctagttgtgt acggctcttt cttaccgtcc
     4741 atgtaaacaa tgttcattgt gcgcctcctt tctaatcttg ataaaataat tcgtctatgg
     4801 ttatatctgg tttaattttc agcatcaagt gcttttagaa gatttcttaa tagttcaccg
     4861 tcagttgtaa ctccaatccc tcctcttcgg tcattatagg taacacggtc agtcgcctgt
     4921 tcgatgagtt tgttgtattt tgctttgaaa tctgcaatgt tcatttgttt tctccttttt
     4981 ttcgcttgtt ctaataggtg ttttcaaatt ggtttagtaa tcttttgcta gccattccat
     5041 aacagaacta taaatccgtt tgggggtcta tttcgatttg ttggtcaatg gctaccaggt
     5101 ttagcccgtg agtggttcgc atttcttcaa tgactttact gtaattcctc cgcattgctt
     5161 gcctgctcct ttcttattct tctggtgtta gtagttcatc aatcgtcacg cctagataat
     5221 cagatacctt ttgtaaggtt tcaatatctg ggcgcttagt acgctcatag tatagtgaga
     5281 taagtgcgct ttttgatata cctgttgcct cgtgaacgtc actaactttt ttacgttgtt
     5341 ttgccaatat gacacgaaaa ttattgttca tgttgtaaaa actccttttt gtttgttttt
     5401 agaaaaataa tagcgccaac gctaaaatta tatcatttat tttacactat atttttatcg
     5461 tgtcaagtaa aaaaataaaa tatattgaaa aaaaggagtg tataggctaa aatatattca
     5521 aaggagcgtg attttaatgc ttagaaataa tttggcaaaa ctaatgattg atagaggcgt
     5581 aacagctacc caattattta atgacaccgg gatagctcgt tcaactattt caaaaatttc
     5641 aaataataat actgacaaga ttagcttgca aacaatagat aaaatttgta actatctaga
     5701 agtttctcct agtgagttct ttgatttttg ggcttatgat gtaaaaattc aatgtggctt
     5761 tgataatttt gatacattat ctgaggtgaa agaacattgg aaatttgttc ctgaatttga
     5821 agaaccatgc tatcttttgc tagagtttag aagagggaga gacagaaaat atcttcttga
     5881 atataaattt atttatcgtt atagcaaaga ccctgataag gcttttgaac caagtcattt
     5941 ggataaagtt cggctaaatg gttctacgtc gcaagttgct atttttgacg atatgcctgt
     6001 gcaatttaaa aatgaattga tagataatat aaagcaacaa ctttctgaaa cgtttaaagt
     6061 atgggatatt tcaccaacaa ttaaatacat agatatgttt attttcaacc caaaagttga
     6121 atagtgtata tcacgcgcca ctatattaaa aatatgaaag ataggttttt ctaggttttc
     6181 tttcaagttt tctgtgtgct acaccttaaa aagtgtctag ttttgtcaag ttttgtaaat
     6241 acatctttca tcatatttca acaaaattag tattttttag taaaatgcgc gtggtacaaa
     6301 attatcttac tcttaccatt ttataaaggc gctcaaatgc tttgtatagc gttttaacct
     6361 caatcaatat aaatagccta tctacccaaa acaaacgaaa ataggggcgt tctcgtaagc
     6421 ctcagcatga tataaaccta ataactcaaa atctttttaa taacggcttg cctgctgata
     6481 gaaaggttca tatcatgaaa attacagaag tcaaaaagaa aaacggtacg atcgtgtacc
     6541 gtgctagtgt ttatttaggt actgaccagg taacaggtaa gagatctaaa acaagcgtaa
     6601 caggtaggac aagaaaagaa actaggcaga aagcaaagca cgcgcaagac gattttattt
     6661 ctaatggcta cacggttact aaagttgtgc cagtaaggaa ttatcaagaa ttggctgagt
     6721 tatggctaga aagctaccaa cttactgtaa agcctcaaac attcatagca actaagagaa
     6781 tgctctataa tcacttaata ccagtctttg gcgctttgaa attaaataag ctatctgtta
     6841 gctatattca aggttttatt aatgatttat ctagtgagtt ggtacattat agcgttgtcc
     6901 actcaatcaa taggcgtgtg ttacaatatg gggtatctct ccagttattg ccattcaatc
     6961 ctgcgcgtga tgttatattg cctaaagtac caaaaaggga aaataaagct attaagttca
     7021 tagctccaga agatttaaaa gcgttaatgg cttacatgga aaagctagcc aataagaaat
     7081 ttagctattt ctttgattat gtcttatata gcgttttact tgctactggt tgccgatttg
     7141 gagaagtagt agccctagaa tggtctgata ttgacctaga gaatggaact atcagcataa
     7201 ccaaaaatta cagtagacta ttaaagttaa tcggtacgcc aaatagtaag gcaggggtta
     7261 gggtcatcag catagacaag aaaacaatca atctgctacg cctttacaag aataggcaac
     7321 gacaattatt tatagagact ggggcgcgtg tttctgctgt ggtgtttgcg acaccactaa
     7381 aggaatatca aaacatggcg accaggcaag acagcttaga caggcggtgt gctgaaattg
     7441 gtatccctcg ctttaccttt cacgctttcc gacatactca tgctagttta ttgctaaatg
     7501 ctggtatcag ctataaagaa ttacaatacc gtctaggtca tgctacgtta gctatgacaa
     7561 tggacattta cggacacctc tcaaaagaca aagaaaaaga ggcagtttct tattacgaaa
     7621 aagccataaa ttctttataa gtccacaaaa aggtgaacaa attttatttt ttgaactcat
     7681 aggcttattg aaaagcctat taaatcaacg tttatagaca gtaaaggagt aaaccatgac
     7741 atttgaagag attttaccag gcttgaaggc caagaagaag tacgttcgta caggctgggg
     7801 tggggcggag aactacgtcc agctttttga caccattgag gtccacgggc aaaagctgga
     7861 ggcgaccccc tatttcctca tcaacgtgac gggcgaggga gaaggcttct ccatgtggag
     7921 tccgaccccc tgcgatgtcc ttgcgaccga ctgggtagaa gtccatgact aagacagccc
     7981 tcatcacagg agtttctagc ggcattggac tagcgcaggc agggattttt ttgggaaatg
     8041 gctggcgtgt gtacgggatt gatcaggcca gcaagcctga tttgacaggc gatttccact
     8101 ttctacagct ggacctgaca ggcgatttcc actttctaca gctggacctg acaggcgatt
     8161 tatcgcccgt cttttcctgg tgccagacag ttgatgtcct ctgtaacacc gcaggcattc
     8221 tagacgacta ccgtccccac ctcgatattt cggaggacga attagctcgc atcttcgcgg
     8281 tcaatttttt tacggtgact agactgaccc gtccctatct gcagcaaatg gtggacagac
     8341 agtcgggcat cattatcaat atgtgctcca ttgcctccag tctagcaggt ggaggaggtt
     8401 cagcctatac tgcctccaag cacgctttgg caggttttac caagcagttg gcactggact
     8461 atgccaagga caaggtccaa atcttcggca tcgcccctgg tgccgttcag acaggcatga
     8521 cccagaagga ctttgagcct ggtggcctgg cggactgggt ggcagaccag acccctatcg
     8581 gacgctggac ccagcccagc gaaatagcag agctgacctt catgctggct actggtaaac
     8641 tcgcttccat gcaaggtcag attatcacca tcgacggcgg ctggagtttg aagtaggggg
     8701 aatggtatga ttcttgaaac caatcgtctc ttgctccgcc cttggagtga gacagacgca
     8761 gctgacttat tttcacaggc cagtcatcca gatgtgggac ccgcagcggg ttggccactt
     8821 catcaaagtc tgaaagaaag tcgagaaatt atcaagacag tccttagcca accagaaacc
     8881 tacgcccttg ttctcaaaga aacgggacag gtgattggca gtatcgggct catgcttggt
     8941 aaacaaagtg gtcttgattt acctgactca gaagctgagc taggctactg gcttggacag
     9001 ggattttggg gacagggatt ggtgccagag gctagtcaag ttttactgga ttacggcttt
     9061 ttccaattga atttagagaa agtctggtgt cgtgcttttg tagaaaatag caaatcatta
     9121 cgtgtccaag aaaagctggg ttttgtttac caatacctct tggaagatgt gcattttcca
     9181 ctcatcgatg aagttcgtac cgaacgcgtg agcttactga ccaaggagga atggcagtct
     9241 agaaaggacc cctatgaccc cgacacaact ctggcaagaa tttcttgcca tcaatcccca
     9301 agctggttct gagccagagg cttgggtctt tggggcggaa gctgaacgac tggctgactt
     9361 ggtagctaga ggcatcaaaa cctcgaccag ctctgcccat gcactctacg cagtagaggg
     9421 agaagaaatt ccaagagctg gtggctatga catcattctg gacggacaag gtcaggctgt
     9481 gtgtattatc cagaccacca aggtctatgt gacgcccttt tccaaagtga cggaagaaca
     9541 tgcctataag gagggcgagt ttcgtcaggg aggtgacttg tctgacagaa aagcgaaaag
     9601 tctgatccac tggcgacagg tccatgaaga gcttttcact atctggctgg aagaagcagg
     9661 cctgtccttc tcagaagata tgctggttgt ctgtgaagaa tttgaattgg tttaccctaa
     9721 atagacaatt ctattggatg caatcaacct gtagtttgtt ttctaatact agaaattgtt
     9781 gagaaactgg ctttattcca gtttcttttt tatttacgaa aattccctat aatactgcta
     9841 ttttccaaaa acatcaaaat ttgctataat agggtatatc attgaaaaga gagaaaacta
     9901 tgagcaataa ctatgcaatt atcctagcgg cgggtaaggg aactcgcatg aagtctgact
     9961 tgccaaaggt cttacacaaa gtggcaggaa ttaccatgtt ggagcatgtc aaacgggctg
    10021 tagatgctat ggaacctgct aaaacagtaa cgattgttgg tcacaaggca gaattggtcc
    10081 aagcagttct cgaaggtcag tcagagtttg cccttcaatc tgaacagttg gggacaggcc
    10141 acgccgttat gatggctgag ccagctcttg caggtttgga aggtcagacc cttgttatcg
    10201 caggagatac accgctgatt acaggagaaa gcttgaaaaa cctcattaac ttccatgtca
    10261 gccacaagaa tgttgcgact atcttgacag ctcaggctga taacccattc ggatatggtc
    10321 ggattattcg caatgctgat ggtgaagtgc aaaaaattgt tgagcaaaaa gatgccaacg
    10381 attttgaaaa acaagtgaaa gaaatcaata caggaaccta cttgtttgat aataaacgtc
    10441 tttttgaagc gcttaaagat attaatacgg acaatgccca aggtgagtac tatctgacag
    10501 atgttatttc gattttccgt caggcaggcg agaaggttgg tgcctatgta ttgcgtgatt
    10561 ttgatgaaag tttgggtgtc aatgatcgcg tcgctcttgc gactgcagaa gctgttatgc
    10621 gcaaacgaat caatgaaaaa cacatggtca atggtgtgac atttatcaac ccagatgcaa
    10681 cctatattga tattgatgtt gagattggtg cagaagctgt tatcgaagct aatgttgtct
    10741 tgaaagggca aacggtgatt ggtgaacgta ctgttttaac caatggtact cgtgtacgtg
    10801 atgcgaaaat tgcagcagat gcagtcatca gcaactcaga cattgaagaa tctgttattg
    10861 aagaaggtgt gacggtagga ccatacgccc atattcgtcc aggcagtctc ttgaaaaaag
    10921 atgttcacgt tggaaacttt gtggaaatca aggcctctac acttggtcaa ggaacgaagt
    10981 ctggtcattt gacctaccta ggaaatgcaa cgattggcaa taatgttaac gttggtgctg
    11041 gtaccatcac tgtcaactac gacggtaaaa ataaatttaa aacaaccgtt ggagataatg
    11101 cctttgttgg ctctaactca accatcatcg caccagtaac tattggggac aacgccttgt
    11161 tggctgcagg ttctgttatt acaaaagata ttccagagga tgccatcggt attggtcgtg
    11221 gtcgtcaaga aaacaaagaa ggttatgcga ctcgttttcc gttccatcca agtcaaaaat
    11281 aggtaatcat atgaattttg aagaaaaaac cattgagcgg acagaaattt ttaaagggca
    11341 catttttgat gtagtagtag atgatgttgc cttgccaaat ggtggaactg gtaagcgcga
    11401 actcattttc cacaagggag cagtttgcgt tttagccgtt accccagaag gtaaaatgat
    11461 tttggtcaaa caataccgca aggccattga acgtactatt tatgaaattc cggcggggaa
    11521 attggagctg ggcgaagaag atacgcttga agatgctgct ttgcgtgaat tagaagaaga
    11581 aacaggctat actagtgaca aactgacctt gttggcagat ttctattcag ccatcggttt
    11641 ctgtaatgaa cgaattcgcc tttatttagc agataacctt atcaaggttg aaaatcctcg
    11701 tccaatggat gaagatgagg ttatcgaatt acatgaggta accttggaag aggctttgaa
    11761 cttggtggct actggcgata tttgtgatgc aaagaccatc atggctatcc aatatttaca
    11821 actcatgaga aagtaggaaa ctagcaatgg gcaagccctt gttgacggat gaaattttag
    11881 agaaagcgaa aaagaaacag tattccgacc aatctagcca gtttggacac tatcgtccaa
    11941 accaggatat atcaatggat gatgctctat tcgatccata tctagaagaa gattatcagg
    12001 gatatgaaga gggacaaacg atccgaatcc cggtcaaacc tactgtagta aaaagtcgcc
    12061 gtatcgaaac catcaagcga gaagctttcc gcagtaaggt aaacaaaatc cttttttggg
    12121 tcatcatctt gctgattatc tttatcattg cagtattatt tatttaggag aagagatgaa
    12181 atttggaatt atcgcagcca tgcctcagga attaaagatt ttggttgagc atctacaaga
    12241 tgcaactgaa atcgatgtct tgggtcggac ttactatcag ggacgaatcg gtcaacatga
    12301 agtcgttctt gttcagtcag gtatcggtaa ggttatgtca gctatgtccg tggctgtttt
    12361 ggtagatcgt ttttcggtta atgtcattgt caatacaggt tcggcaggag ctgttgctga
    12421 tggtattgcc atcggtgatg tggttgtagc aaatcaattg gcttaccacg atgtggatgt
    12481 gacggctttc ggctatgctt acgggcaaat ggcagggcag gaattgtatt atccggcaga
    12541 ccaagtcttg ttagaacagc taagaaccgt tttagcagag caggaaatga ttagccatgt
    12601 gggtctgatt gtgacaggtg acagttttat cgcaggtcag gaaagaatcg ctacaatcaa
    12661 aactcatttt ccagaagtcc ttgccgtaga gatggaagga gcagctatcg ctcaagcagc
    12721 tgtcaataca ggtaagccat tcttggttat ccgtgctatg agtgacacag ctcagggaga
    12781 tgccaatatc acctttgatg aatttatcat tcaagcagga gaacgttctg cccagaccct
    12841 gattgccttt ctggagaaaa attaagcaga ttttgacaac aatacattga aataggatga
    12901 cagtagcaaa aagttgttac tgtttttctt atgcagtaat ttgagcccga tattataagg
    12961 tttcagccat ctgtccccat acaatttctg ataagagtta ttgaaaagct cgtgaaaata
    13021 ctttacaatt ttgtttaaga aaaataattt aggaggttgt tatggttcag cgattattgc
    13081 ttgcaaatga tttgcctggt gtgggtaagg ttgccctagc cactagcatc ccaattgcag
    13141 cagcctgtca ggtagaaacg attcttttac caacggtact actttcttct catacgggtg
    13201 gttttccaga tgttgtcgtt gaagatatga ctgacttgaa tcgagcctat tttgaacagt
    13261 ggaagaactt gaatttggag ttggcaggca tcttgtctgg ctactgtaga aatcctcagc
    13321 aactaagtca attggctatg tatgcgaaac aaacagagac accattgatt gttgatccgg
    13381 tcatggggga tggagggaag ttgtatacag gtttcacatc tacctatgta gaagccatga
    13441 aagagttgat ccagtcggcg aaactcattc tccccaatgt aacagaggca gcttttttga
    13501 cagggaggga ttatttggga gcttgctacg atagggcagc tattgaggac ttactggaag
    13561 ctttagcaga gcaggtatcc gcagatattg tactgacagg gatttccttt gatgatagcc
    13621 aaataggtgt agcctatttt cagcaggaaa caggggaaat aactacctat atgtctaaaa
    13681 aatatccagc aaatttcttt ggaacaggtg acattctatc taccttgttg gcagtagcaa
    13741 ccatccaaca aatcaatctc catcaagcta tccctctagc tttagatttt atcgataaga
    13801 gtttagagcg tacccttgct ttggatagag acttgaaatt tgggatttcc tttgaacctt
    13861 ttctagcaga attacagcag gcatttcaaa cattaaagga gaaattatga aacagagtca
    13921 attgcgtcaa ttaacagaaa ttagcctatt tgcagccctt atttgtgtgg ttgtcttctt
    13981 tattcgtatt ccttttggga gtcaatttgt ccatttaggc aatgctctgg tggtagtagg
    14041 agtattgatc tttggagcca aaaaaggggc gctggcagcc tcactcggtc taggcatctt
    14101 tgatttactg tcgggatatg cttccgttgc ctggattacc attctagaat cactcattgt
    14161 ctgttttgtc ctccacctat tttatgagaa attcctacag gcaaatgata gtctatcaaa
    14221 tattatgttg gttgggatag ttgctgccct cacaaaaatt atcttgaacc tgttgaagta
    14281 cacattgacg ggtagtttag tcggtggact aacgccattg gcatcatttt tagctgcttt
    14341 gggtcggatt ttaggaacct atggcactgc aattacgacc atcattgctg tacctattct
    14401 ttataaggta tgtaaaaggt tatacggtag aaagtaattg agaaatttga aaatgcaaaa
    14461 aagaggctgg acaaaaagcc cagcaccgct tctcaaagtt agtgtcaaca tctcagcgca
    14521 gtggttgatt ggcagatttg ttcgtttttt gcactccaaa tctggcctaa tcaactgtgc
    14581 gggggcggga agacgaactc tttttatctt ggtcgagttc tttcccactc ccgctccttt
    14641 aattatttat tataaataac tgaattatca aattaggtta gactttttaa gtagctgaga
    14701 aagacttggt aatgtgtttg ataattccta gaaaatcctc tgtttcgcct tgataggaca
    14761 tttgaatgtt cattgagacc tcgtttctca ggacaagcct tggtctgcaa agaaaatatc
    14821 aaaggtacat tcgaaatagg tattcaggta gagaattctt tcccacaatc aaaggttatc
    14881 gttttagaga gaggattgag gacttgagat aaccactaat tgatggaagc tccaatatca
    14941 cttgactttc gtccattgct ttttagtgtg atgatggcgt tctatgtcta gctagtatac
    15001 tattttacgt aatctttcag aatctcaata gactcaattt ttacatctgt tttaggttta
    15061 tcgttattgt ctgtttcgac tgaagcgatt ttatccacga tgtccatccc ttcaatgact
    15121 tgaccgaata cggtatgctt gccatctaag tttggattgc ctccgttttt ataagcgtca
    15181 atgattttac caggatagct agaactagag agttggctag acatgtctgt tgtattttga
    15241 ttgatgaaaa actgacttcc atttgttccc gcaccagcat ttgccataga aagtgagcca
    15301 cgaatgttat aaaggaaagc tgaaatctcg tctttaaaac catttccagc atcgatagta
    15361 gtatcttttc cggcccagat agactctcca ccagtaccat ttccaagagg atcgccacct
    15421 tgaatcatga aatccttaat gacacgatgg aagatcgttc cattatagta tccttccttc
    15481 gcatgagtca gaaagttttc tacagttagt ggtgcttgct cggggaagag ttttatagtg
    15541 atgtcgcctt cggttgtctt tattttaaca gcagcctcat tttcaccaat ttcattggaa
    15601 agttggggga atgtagcttt gggattgtta atggcgtatg ccaaatcttt ggcatattgg
    15661 ctagaagcag ttgtatcagc tgtcgtagtc gagcttggac tagaactaga tgctgtagaa
    15721 gatgacctat tagctgtcga gttgtttgtg caggcagtta gcaagatgct cgaagctaga
    15781 gcaagagcta gtattttttt cattgaaaat cctttctcgt cttgttcaaa attatgctca
    15841 ttctaccatg aaatatggat tttttcaatt tttatctagt ataaaaagcg tatctaaaac
    15901 aataatatga tatactatat ttacagatac gaaaggagaa taatgatgtt tgagaacttg
    15961 tttgccccag atggaagcct agtatggcat atggcccttt tctacagttt ttccgcgatc
    16021 atattagcga cggtttgttt ccatattatt aaaaatttta tggaatggca ccgcaacaat
    16081 cgggagccca aagaatcttg caaggctaag cttgtctcta aacgaaccca tgtttttggt
    16141 aacgaagtag cacgaaccaa ttattatgta acgtttgaat gggagggaaa acggcaggaa
    16201 tttcgccttc gttcagacga atatgccctt ttagcagagg gagatgtagg caccctgcat
    16261 tttcagggga ctcgtttcct aggttttgaa cggctctaag agtcgttttt ctttggagaa
    16321 tatctctatc tagtatataa tggtttgaac acatgttcgt acaacgcaaa agaggtattt
    16381 aagatgattc aatcaatcga acaagttatg ttatatgtga atgatattga ggcttcagct
    16441 caattttgga aagagaaaat gggatttgaa cgtgtagaaa aacaagtgca aggcccccaa
    16501 acttcttaca ttattgcacc taaatcagat agtgaggtac aatttgtact tcatgataag
    16561 gcagaggttg ctgaaatgga gccagaatta aacttaggta taccatcaat cttaatgaca
    16621 tcagtagatg tagccaaaac ttacgaagaa cttgttgaac gtggtgtcac aacgaatcca
    16681 gtcatggacc ttggtttcat gaaggtaatt agctttgcag acaatgatgg aaattattac
    16741 gcaattcgtg aggtaaagta aatgtcactc cagaatcgtg ttgaagaaat gtataaagac
    16801 cacgaggtca aaccctatat ctcccccgag cgtgacttgg cagcctggct gttggaggcc
    16861 aaacccgttc ccaaacgcaa tatggttcgt ctggaagaag gtattttgcc aggagatatt
    16921 attttgctat ggcggattag cctgggttct tttgaaacga cgacgcccta ctccaagtac
    16981 tttgagtaca tgtatggtat caatggtcct gctcacatgg agcagttgat tgcagacggt
    17041 tatgtctatg tggagtcagc ctttgattcc ctagaccaca tcacttcgac tgccaagaaa
    17101 aatatcctca aagcagaggg tgtgacaggc ctatccaaga tgaaggcggc agacctcgac
    17161 actgccctca aggacaactt gacggaagaa aagttagctc catactttac cgtccgaggt
    17221 tatgccctga cggaaaaagg cagagctgca ctcgataatc atacagaagt acttgcaaaa
    17281 cacccaatga agaagatgta caaataaaat agaaaagatt tttaaacggc acccaaaagt
    17341 tagaatcatt ctagtttttt gggtgttttt atttgctaac aagtcaattt ttcttgacag
    17401 aaaacgctat caatgatatt ctagtgtaaa ttattgaaac gtttcaaaag aaaggggtaa
    17461 accatggaag agcaaaaatc aatcaccatg aaagacgttg cgcgagttgc tggtgtaagc
    17521 gttgggactg tatcaagagt tatcaacaat gagccaggta ttaaggaaag tacgcttgaa
    17581 aaagtcaatg ctgccattaa ggaattgaat tacatcccag atgtctatgc tcgtggcatg
    17641 aaaaagaaca agaccgaaac cattgccttg attattccaa cagtttggca tccctttttt
    17701 ggagaatttg cctatcatgt tgaagtggaa ctgagtaaga aaaattacaa gttgctcctt
    17761 tgcaatattt ctggaccaaa aagggaactg gactatctaa ccatgctgca acaaaataag
    17821 gtggacggta ttatcgccat cacctatagc ccgattgacg attatctatc gtccaatatc
    17881 ccctttgtca gtatcgatcg cacttatgaa aacaaggaca tagcttgtgt cagctcggac
    17941 aaccaagctg gtgcggagct agcagcggac atcttgattg aaaaaggcgg tagccaattt
    18001 gcctttattg gcggacacaa taaaaccatc aacgaaacca agaagcgtag attgtatttt
    18061 gaaaagcgaa ttttagaggc tggttttcct tgtcaagtct tggatttgga agaaccctat
    18121 gatgattttg ttggaacagt agagaaattt ttgattgcca acccgcaaat tgatgccatt
    18181 tttaccatca atgattttgt ggctcttgac accctttcca ttttggaaaa actgggacga
    18241 cgggttccag aagatgtcca gatcattggc tatgacggta ttcagttggc gggcgaacgt
    18301 tccttagaac tttcgactat tcgtcaacct ttagaggcta tggcccaaga agcagttgcc
    18361 tgtttagtcg acattattga gaaaagggag cgtcctctcc aagtcacatt accaatttct
    18421 tacctagaag gaaaaacgac aaaaaatttt taaaaagcag ttgacagaaa acggttacaa
    18481 tgataaaata aaatcatcaa taaacagttg gttttatttt tacaagaaaa atgaaacgtt
    18541 tcaaaaacgg aggatttggg ttcatctttc taaaaccaag gattttgaaa cgtttcaaaa
    18601 aggagttctt atgaaaacat caaaactgca acaagctagt ctagtagaca gaataaagca
    18661 acagaaactc ttgttactga tgttgctacc aggtttggtc ctgacattca tcttccgtta
    18721 cattcctatg tacggggtgc tgattgcctt caaggactat aaccctttaa aaggtgttct
    18781 aggtagtgag tggattgggt ttgaagagtt tacaaagttt ctctcttctc caaactttgg
    18841 taatttgctt gccaacactt tgaaattgag tgtctacggc ctgctactag gatttcttcc
    18901 tccgataatt ttagccatca tgctcaatca actattgagc gataaggcta agaagcggat
    18961 tcagttggtg ctctacgctc caaactttat ctcagtcgtg gtcatcgtcg gtatgatttt
    19021 cctcttcttc tcagtaggag gaccggtcaa ttccatccta ggtatgtttg ggttagaagc
    19081 caacttcctg acgaatcctg attacttcag acctctctat attctcagtg gtatctggca
    19141 gggaatgggt tgggcatcaa cgctctacac agcaaccttg gtcaatgttg atccagcctt
    19201 gattgaagca gcaaaactag acggtgccaa tatcttccaa cgtatctggc atattgactt
    19261 accagcacta aagccagtta tggttattca gtttatcctg gcagctgggg gcatcatgaa
    19321 cgttggttac gaaaaagcct tcttgatgca gacttccctc aacctgacca gctctgaaat
    19381 catctcaacc tatgtctata aaatcggttt ggtgtcaggt gactactctt actcaacagc
    19441 ggttggtttg ttcaatgcct ttatcaatat tatcctactg attgcagtca ataagattgt
    19501 ccaacgcatc aatgatggac aaggcttata ggaggttcgt atgaatacac aaatgtacac
    19561 aaaatttgac aagcgaatgc tgattgtcaa taaaatcttg ctggcatttc ttgttctcat
    19621 aacagttgtg cctatggtct atatcctagt cgcttccttt atggatccgc aggccttggt
    19681 cagcaagggc attagctttg atccaaagga ctggacagta gaaggttacc aacgggtatt
    19741 ttcagacaag tctatcctac gtggattttt caactccctc ttttattcct tctcctttgc
    19801 cttcgtgacc gtggctatct cagtcatgac tgcctacccg ctctctaaaa aagatttggt
    19861 tgggaaaaaa tggattaatg cctttctagt tttcaccatg ttcttcggtg gtggacttgt
    19921 tccgacttat ctcttggtca aagatttggg aatgctgaac accgtttggg ccattattat
    19981 tcctggtgca gtcaatgttt ggaatatcat cttggcacgg acctatttcc aaggcttgcc
    20041 agatgagttg gtagaagcag ccgttatgga cggtgccaat gaatttcaga ttttctggaa
    20101 aatcatgatt ccattagcta agccaatcat gtttgtgctc ttcctctatg cctttgtcgg
    20161 tcaatggaat tcttactttg atgccatgat ttacatcaag gatccagact tagagccact
    20221 ccaactagta cttcgtaaaa tcctcattca gagcgagcct gctaaggaca tgattggtgc
    20281 tcaggctgct atgaatgaaa tgaaacgcat cgcagaaatg attaagtatg cgaccattgt
    20341 catctctagc ttgccactca ttgtcatgta tccattcttc cagaaatact ttgacaaggg
    20401 tatcatggct ggttcattaa aaggttaata ctcttcgaaa atcaaaagca gacgttgttg
    20461 acttgatttg atgaacgtca gttctatctg catctgcgtc gcctagtctg tttttgattt
    20521 tcattgagta taataaatat taattatttt ttagaaaaca atacaggagg ttgtttctga
    20581 tgaaacacac atttggaaaa acagttgtta cgcttctagc aagtaccgtt ttattggctg
    20641 cttgtggctc aaaaaatact gcttcaagcc cagattatga attgaaagat gttcaattcc
    20701 cgcttgaaaa atcagttacg cttaagttta tgacagctag ctccccccta gctccagcag
    20761 atccaaatga aaaattaatt tttaagcgtt tagaagaaga aacaggtgtg catatcgatt
    20821 ggacaaacta ccagtcagac tttggtgaaa agcgaaactt ggacatcgct tcaggtgact
    20881 tgccagacgc catccataat gacggggctt ctgatgtgga attgatgaac tgggctaaac
    20941 aaggtgttat tgtaccagtt gaagacttga ttgataagca tatgccaaac cttaagaaaa
    21001 ttttggatga aaatccagaa taccgttcaa tgattacggc tccagacggc catatctact
    21061 cattcccatg gattgaagag ttgggtgaag gtaaggaatc aatccacact gttaacgata
    21121 ttgcttggat caacaaagaa tggttgaaca aactcggtct tgaaatgcca aaaacaacgg
    21181 atgacttgat taaggtcctt gaagccttca agacccagga tccaaacggc aatggcaagg
    21241 cggatgaaat cccaatgtcc tttgtcaacg gtaacggcgg cgaagacttc aagattctct
    21301 ttggtgcctt cggtgtcggt gacaacgatg accatatcgt cgtaaacaat gacgggaccg
    21361 ttgactttac agcggacaat gaagactaca aagaaggtgt tggatttatg cgtacccttc
    21421 aagaaaaagg tcttctagat cctgaagcgt ttgaacaaga ttggaacact tacatctcta
    21481 aaggtagtga aaatctatac ggtgtttact tcacttggga taaagctaac attacaggta
    21541 tgaatgatac ctatgacatc ttgccagtct tggcaggtcc tgacggaacc aagcatatca
    21601 cacggaccaa cggtatgggc ttccaacgtg accgcatggt catcacatca gctaataaaa
    21661 acttggaatt gaccgctaag tggattgatg cccagtatga accaatccag tctgtccaaa
    21721 acaactgggg tacttatggt gatgaaaccc aacaaaatat ttttgaactc gacacagcaa
    21781 ccaacagtct taagcactta ccattgaatg gtaccgcacc gggtgaactt cgtcagaaga
    21841 cagaagttgg tggaccgttg gctattcttg atgaatacta cggaaccgta acaaccatgc
    21901 cagatgacgc taaatggcgt ttggatttga tgcatgcgac ctaccttgac tatgtaacaa
    21961 acgatgatat ttatcctcgt gtcttcatgg aacaagaaga tttggacaag attgcccaag
    22021 ttgaagcaga tatgaacgac tatatctacc gtaaacgtgc tgaatggatt gtaaacggtg
    22081 gtgtagagga agagtgggat agctacttga aagagttgga aagctatggt ctttctgact
    22141 ggttagctat caagcaaaaa tactacgatc agtataaaga aaatcagtaa tcaaaaggag
    22201 gctgcctgtg aaaacagtag tagaagccaa tcaatttatt cagacagaaa aaggaaacgt
    22261 caatccgatt ttcaaaccgc aagcccattt aacaccagaa acaggctgga ttaacgaccc
    22321 caatggattt atctacttcc gtggagaata ccatttgttc taccaattca acccttacga
    22381 gagtgtttgg gggccaatgc attggggcca cgccaaaagt aaggatttgg tcaactggga
    22441 acaccttcca gttgcccttg ctccagacaa ggactatgat aaggacggct gtttctcggg
    22501 ctctgccatt gtcaaagatg atgttctatg gctcatgtat accggtcata tcaacaatga
    22561 agacgggaca gttagccaag ttcagaacat ggccttctca actgacggta ttcactttga
    22621 aaaaatcgag cagaatccag tcgcaacagc agatggactg ccagaagaag tgattgccaa
    22681 tgacttccgc gatccgaaga tttttgagaa agatggtcac tactactctg ttgtcgcaac
    22741 caagcacaag gataatgtcg gctgtatcgt cttactcagc tctcccaacc tgacggattg
    22801 gaaatttgag tctatcttct tgaaaggtga ggccaaccaa ggctttgtct gggaatgtcc
    22861 tgactacttt gaagtggacg gtcaagagta cctgattatc tctccaatgc gttaccaaaa
    22921 agacgggaat gactttatca atatcaactc caatatcttt gtgacgggtc atgtagactg
    22981 ggacaagaaa gtctttgtgg cagaatcctt caaggaaatc gaccatgggc atgatttcta
    23041 tgcagctcaa acgactgaag gtccagaagg ggaacgtgtc atgattgcct ggatgcatac
    23101 atggggacgg ccgcttgtaa ccaatgacct aggccataag tggtatggtc aaatgaccct
    23161 gcctcgtcta cttaagcaag gtgagaatgg actcagacag gtcttgccag caggtatctt
    23221 gaacagcttt gacgacattg aaatcggtca agttattcaa ggaccaagta aactttcact
    23281 caaactggac gacagtctag acttgaaact ggggactgac cgagactatc ttcaatttgg
    23341 ctatgataaa gccaaacaag aagtctatat cgaccgtagc tctctcaaaa tccagcaagt
    23401 tggtgaggag gaatggtcaa ctgttcgtcg agctgtcacg gttcaagcga cagagctaga
    23461 tgtcttggtt gacaccaact gtttggaaat ctttattaac gatggtcaag aaaccttgac
    23521 atcaaccttc tacgtagaag gtcagattat tctaaacgct ctataatatt atttgccctc
    23581 ttaaattatt ctttcactcc aagagaaacc ctgtatttgc agggtttttt ctatcatgaa
    23641 aattcttccc aattatgata gaataaagag attgagaaag ttgaggtatc tatgagaaag
    23701 acaagtaaaa aaggaaagtc tacgaggcga ccaaccaagg cagaattagc acagcaagaa
    23761 cgtgttaaaa atatcaccct tcgagtattg gggcttttat tcattgcctt tgcagccagt
    23821 cgcctaggtg tgtttggagt gaccagctac aatattttcc ggctattatt tggtagtctg
    23881 gcctatctat tattggcggg tgcatttatc taccttctta ttcccaaagt cttacgcgaa
    23941 agagaaggga ccatatcggg cttctggctg attgttatcg ggttattaat tgaatttcag
    24001 gcttacttag actggaccta tcaagggagt gacttattcg gtcatactct aaaattggcc
    24061 ttgtcagacc tagctaagtt tcaggtaaca gcatttctag gcggagggat gattggaagt
    24121 atcttctatc tgccagtctc cttcctcttt gcaaatgtcg gttccttctt catcggtctt
    24181 ctagtgatag catttggtat cttttttgta agcccatggt ctgtttatga tgttgccgat
    24241 ggcttggcgg ttgccaaaga taagttggtg gagacgcagg taaaaagagc agagcttcga
    24301 acacagaaga aggctgaacg agaagaaaaa cgtcagcaag aattagctcg tctagaggaa
    24361 gagtcagctc gggctttggc actggtagat agacatgtcg atttagaaac gggtgaaatc
    24421 ctcgaagaag agcctgttca agtaccgatt gtttcagaat atgaccactt agacatagaa
    24481 gatgaatttc caattatgtt ggcagaagat gaccaaccag cacaatcaaa ctcggcaagc
    24541 caggcttctg aaccaaatga gtttgagaag gatgagacag atgtgcagat tgatttcaag
    24601 cctaagcaga gactagcata taaacttccg tccatagact tatttgctcc tattaaagct
    24661 aagagtcagt ccaatgaaaa acgaattgtt cgtcagaata ttaaagtttt agaagacacc
    24721 tttgccagtt ttggaataaa ggtagtagtt gagcgtgctg agattggacc atcggtaacc
    24781 aagtatgaag tcaaaccagc agtcggcgtc cgtgtcaatc ggatttctaa cctagctgat
    24841 gatttggctc tagcccttgc ggctaaagat gtccgtattg aagctcctat cccaggaaaa
    24901 tctctggtcg gtattgaagt tccaaactcc gaagtggcta cagtaccttt ccgtgagctc
    24961 tgggaacaat ctaaaacaga cccagacaag cttttggaaa ttcctctcgg taaggctgtc
    25021 aatggctccg tccgttcctt taacctagca cgtatgcccc atctattggt ggctggttca
    25081 actggttcag ggaagtctgt tgctgtcaac ggtatcatct cttcaattct catgaaagct
    25141 ggtccagacc aagttaagtt catgatgatt gaccccaaaa tggtcgagtt atcggtctat
    25201 aatgatattc cccacctatt gattcctgtt gtgaccaacc cacgcaaggc tgctcgagct
    25261 cttcaaaaag tagtggatga aatggagaaa cgctatgagt tgtttagcca gattggtgtc
    25321 cgaaacttgg aaggttataa tgcgaaggtc gaagaattta atagccgttc agaagaaaaa
    25381 cagattccac ttcctctcat tgtggtaatc gtagatgagt tggcagactt gatgatggta
    25441 gccagcaagg aagtggaaga tgccattatt cgcttgggac agaaggctcg tgcagcaggt
    25501 atccacatga ttcttgctac ccagcgacca tctgttgatg ttatttcagg tttgattaaa
    25561 gccaatgtcc catctcgtat cgcctttgcc gtatcaagcg gtacagatag tcggacgatt
    25621 ctagatgaaa acggtgctga aaaactcttg ggccgcggtg acatgctctt taaaccgatt
    25681 gatgaaaacc atccagtccg cttgcaaggt tcctttatat ccgatgatga tgtagaagca
    25741 atagttggct ttatcaagga tcaagcagat gcagattatg atgaatcctt tgatcctggt
    25801 gaagtggcag aaggtgatgg cgatacaggt tttggtgatg ctggaggaga tccacttttc
    25861 aacgaagcac gtgccttggt agttgagaca caaaaagcca gcgcctctat gattcaacga
    25921 cgcttgtctg ttggttttaa cagggcaaca cgcctcatgg aagagctaga agcagcgggt
    25981 gtcattggtc cagctgaagg aacaaaacct agaaaggtat tggaaacaca ataatgaaat
    26041 ttgatgcagt tcatcatatt gcaattatcg gtagtgacta tgacaaaaca agagaatttt
    26101 atgttgagaa actaggcttt gaacaggtgg atgagcatat tcgcccagaa aaaaatgata
    26161 ttctcttcaa tgtcaaaaaa ggcaatctga tcttggaaat tttcatcaaa ccggatgcac
    26221 caatgcgtcc ggccatgccc aatcctgaac atacaggtct acgccatcta gccttccagg
    26281 ttgcagatgt ggaagcttgt ctggaagagt ttgaccgttt ggacatccga catgaagtcc
    26341 tgcgaacaga tgattttgat ggtaaaaaaa tggccttctt ctttgatcca gatggcctcc
    26401 ctctagaaat tcatgaataa gtccatcttt tcatgcccag agaggccttt caatatgaca
    26461 actaattaat actcttagaa aatcaaaatc atacgctgtt gacttgattt gatgaacgtc
    26521 agttctatct atagtcattt gaattaactt tgatacatcg gcgctttcgg cttgccgtac
    26581 tctgcagaag tgacttgctt tgcaagttat atctccaacc tagcacagcc actaggctgt
    26641 gctagcaacc tgcacctcag ttccttgtct gaaagctaat tcattcgact atacatctac
    26701 gtcgccttgt ctgatttagt ataaaaaaga atccccactg cgcctgcaat ggggatttat
    26761 tatgccatct atgaaaggat ggatgatttt aggattttaa agtgaagacc gcaataacca
    26821 aagctagata catcaaaaag gtcaagataa aactagctcg ccagaaaacc ttgaagaaac
    26881 ggcgatataa aaaattcttt ttctttaaaa ggaagaaacc acaaagacca atcgctagta
    26941 gtgagagggc taggacaagc tgaggcagta gactgttata gtaggccttg tccgaaatca
    27001 gataaaattc aattgcatag agaggaaagg caatgtctgc aaagttccat cctcggtttt
    27061 gcaacctaaa aaacttgaca gcaattatcg aaatggccaa ggttagaaag ataaatagga
    27121 aggctactat ttttaaaaat ataatcgatt caaacataca ttcattctat aaaaaatgtg
    27181 aaaaaaagta aacctaaagc ttgcatttct ttgaaattcg tgtataatga aaaggtatgt
    27241 gtaaaacaca tatttgtggg aggtaaaaat ctttaattac cgccaaaacc acaataggag
    27301 gattttatat catggctaaa aaagtcgaaa aactcgtaaa acttcaaatt cctgcaggta
    27361 aagctactcc agctccacca gtcggaccag cacttggtca agcaggtatc aacatcatgg
    27421 gattcacaaa agagtttaac gctcgtacag ctgaccaagc tggtatgatc atcccagtag
    27481 ttatctctgt gtatgaagat aaatcattca ctttcatcac taaaacacca ccagctgctg
    27541 ttcttttgaa aaaagctgca ggtgttgaaa aaggttcagg tacaccaaac aaaactaaag
    27601 ttgcaacagt tactcgtgca caagtacaag aaattgctga aactaaaatg cctgacttga
    27661 acgctgcatc tcttgaagct gcaatgcgta tgatcgaagg tactgctcgt tctatgggat
    27721 tcactgttac tgactaagta gcacccaaga ttatctgatt acaaggagac atataacatg
    27781 gctaaaaaaa gcaaaaactt gcgtgctgct cttgaaaaaa tcgacagcac aaaactttac
    27841 agcgtagaag aagctgttgc tcttgcaaaa gaaactaact tcgctaaatt tgatgcgtca
    27901 gttgaagttg cttacaactt gaacatcgac gtacgtaaag cagaccaaca aatccgtggt
    27961 gcaatggtat tgccaaacgg tactggtaaa acttcacgcg ttcttgtttt cgcacgtggt
    28021 gctaaagcag aagaagcaaa agctgctggt gcagactttg ttggtgaaga tgacctcgtt
    28081 gctaaaatca acggtggttg gttggacttc gacgttgtta tcgcaactcc agatatgatg
    28141 gctgttgttg gacgtcttgg tcgtgtactt ggtccacgta acttgatgcc aaaccctaaa
    28201 actggtacag taacaatgga tgttgctaaa gcagttgaag agtctaaagg tggtaaaatc
    28261 acttaccgtg ctgacaaagc aggtatcgta caagctctta tcggtaaagt atcatttgac
    28321 gctgacaaac tcgttgaaaa cttcaaagct ttccacgatg taatggctaa agctaaacca
    28381 gctacagcta aaggtactta catgacttca gtatcaatta caacaacaca aggtgttggt
    28441 atcaaagttg atcctaactc actttaatca atagaaagat tgtctatggg caatcttttt
    28501 tagttgacat tcaataaaac aaactccata atatatgtat aaagtgattg aggaggcaac
    28561 tatgaaggcg acctgtgaag aattttatat tcatctttcg gcggtttatc agctaccaga
    28621 agatgctatc acatccgttt tgcgtgaaaa ggtttttgaa actgccaaag agcttgctca
    28681 agctgacaat ctctacttgc ttgctgaacg gttgggacac tatgttaatg cagagctgac
    28741 agccttgact tgccttacac caaaagagtt ggttcagctt tctctttaca ttcaacaact
    28801 tcaaaatcac tatcggatag caagttttat cccaggaaag gtagaataaa aaagatgaca
    28861 gaatatacaa aaccacttgt ttggaagtgg gaagatgaaa atggcaaccg ttctggcaac
    28921 cgtccaacag cgggcagtcg ctttgagcaa gacctaccaa agggagagaa gtcttttcaa
    28981 gtctactcat tggggacgcc gaatggcatt aaggttgcta ttatgttgga ggagttgaaa
    29041 gagttaggta tttcagaagc tgattatgac ctctttctca tcaatatcgg tcaaggagac
    29101 caatttggct ccgattttgt tgccatcaac cccaactcca aaataccagc tatggtagat
    29161 tattccgagc aagaaccgat ttcggtcttt gaatcagcca atattcttct ctacttggca
    29221 gaaaagtttg aggccttcct accaaaagct tgggcagagc gggcagaggt tcttaattgg
    29281 ctcttctggc aaactggcgc cgctccattt gttggtggag gatttggaca tttcttccat
    29341 tacgctccga cagcgcaaga atatccgatt aatcgctata caatggaaac taagcgtcag
    29401 ctggatctct tggatcagct attggcgaca agagaatata tagctggaaa tacctatacc
    29461 atcgcagata ttgctatttg gtcttggtat ggtcgtttgg tgcaaggaga actatatcca
    29521 ggttcggcag aatttttaga tgttgattct tacaagcatc taaagacctg ggtagagaaa
    29581 atagctcagc gcccagctgt acaacgaggg ttgacagtga aatatcattc aattgataat
    29641 taattgactt gcccacctta tgggtgggtt tttcttttac tttggtatga caaaagttta
    29701 taatgaaaga gttagacttt gtgaaaatcc aaaaatagaa attaataatt gatactcaat
    29761 gaaaatcaga attagactag gcgacgtaga tgcagataga actgaagttc atcaaatcaa
    29821 gtcaacaacg tctgattttg attttcgaag agtatgagtt tttcctgtct tatatttaca
    29881 tagaaagtaa tagaaaaatg tccgaaaaag ttgaaatgca gcagtccaaa gaacaggcga
    29941 caatggccag aggcttggct tggttgactg ctggaaatat tcttagtcgc ctgcttggtg
    30001 ttgcctatgt tattccctgg tatatttggt taggggaata tcgggctgaa gccaatgccc
    30061 tctttagtat ggggtatcaa atctatgcca acttcttatt gatttcaaca gctggtcttc
    30121 cgacagctat tgctaaacaa gtagctaaat acaatgtctt gggtaaagaa gaggtgtcgc
    30181 tttacttggt cagagaattc ttcaagctca tgttggtctt tggagctgtc tttgcagggg
    30241 tcatgtatct aagtgctccg tggttggcag acgcatcggg gtcgaaggaa aagttacttc
    30301 cagtcatgta tagtctagtc ccgcctctct ttattttccc agccatgagt atcttgcgtg
    30361 gttttttcca aggtcggcac gatatgaagc cctatgcgtt cagccagctt gcagagcagc
    30421 tggtacgggt tatctggata ctggcagcaa catttatgat tatgaagttg ggtagtggtg
    30481 actatcttga agcagttgtc caatctacct ttgcagcttt tgtcgggacg attgctagtg
    30541 ttgggatttt agtttatact ttatggaagc aaggttacct tggtaagttg cttcatgcta
    30601 aaaaacaaaa gatttctttg gatacaggtc aacttattaa ggaaactgtt cgagatgcga
    30661 ttccaattat cattttaggt ctaacgattc aattattgca gtttattgac caagtctcat
    30721 ttattcgtgt tatggaaaga attacagatt acagtaattc ggaacttttg gaactttatt
    30781 catatatggc tgccaatcca agtaagatta cgatgatgat tatcgggatt tcactgagtt
    30841 tgggaagtgt tgctattcca ttgattacag agaaatttgt caagaaagat ttgaaggcga
    30901 cgtctcattt agtggcggat aatctacaat tactctttat attcactata ccggcaattg
    30961 ttggaacagt cctgttagct gggccactct atacgatttt ttacggaccg tcagagccaa
    31021 tcgccattac tctatttatt tggaacttat ttttaatttt acctctggga ctatattctg
    31081 tcattagtgt ggtcattcaa gcgatatttg aaaatagaag agccatttat tatttcttga
    31141 ttgggatgct agtgaaaatc gttttacaag taccaatgat ctatgtcttt aaggcatatg
    31201 gtagctttat ctcaacgata tttgggttgg gaattatgct gtacctcttc tatagacgaa
    31261 ttgacaaggt tctgcatatt gatgaaagac tagtgataaa agatattgcg accattagtt
    31321 ggatttctat cataatggga ctcgttgtat gggggattga atttgtcctg aatcttatcc
    31381 ttccagcaaa tggctatgta tctagcttta tccacttggc tgttgcaggt ggagcaggta
    31441 tgcttgtctt tgtcatcttg acattgaaga ctcgtcagct agatcgttta attggaagtc
    31501 gggctcagag tttacggagg aatttgcgcc taggctagtt tgttttaaaa atctctattt
    31561 tctactattg aaaagctggg aaaaagaggc tttttagaat gtgagaagga aagttcgttg
    31621 aggattttcc ttttttcttg gagatttcag tataaaaaaa acggtttttg tggtagaata
    31681 gtagggataa aaaagaggag agagatgatg aaacctaaat acgaacgtat tctaatcaaa
    31741 ctatctggtg aggccttggc tggtgaacgt ggtgtcggta ttgaccttaa aactgttcag
    31801 gaaatggcaa aggaaattca ggaagtcgca gaatcaggta ttcagattgc ccttgttatt
    31861 ggtggtggta acctttggcg tggagaacct gctgctgaag cgggtatgga tcgagttcaa
    31921 gcagactata caggcatgtt ggggactgtt atgaatgctc ttgtaatggc tgattctctt
    31981 aaacagcttg gtgtggatac acgtgtgcag acagcgattg cgatgcagtc agttgcagag
    32041 ccttatattc gtggtcgtgc tcttcgtcat cttgaaaaag gtcgcattgt tatttttgga
    32101 gcaggtattg gttctccgta cttctcaacg gatacgacag cagctcttcg tgctgcagaa
    32161 attgaagcgg atgcgatttt gatggctaaa aatggtgtgg atggcgtcta caatgctgat
    32221 ccgaagaaag atgctaatgc agttaaattc aatgaattga cccaccgtga agtgattagt
    32281 cgtggtttga aaatcatgga tgcgacagca tcaactctct caatggacaa tgatattgat
    32341 ttggtcgtgt tcaatatgaa tgaaccagga aatattaaac gtgttgtctt cggtgagcca
    32401 atcggtacta ctgtttcaaa ttcttcagaa gaaaaataaa tcatatcgtc atttagtttt
    32461 tcttttatta cttcgttaat ctgccttgcc taacttcagt tatgcctgtg gctcgttgcc
    32521 tagtgctaaa agtaaactaa aagactataa aaaaatacaa agaaggaagt cttatgtcta
    32581 aagaaattat tgctaaagcg caagagcgca tgaaccaatc tcatcagagt ttggcacgtg
    32641 aattcagcca cattcgtgct ggccgtgcca atgctagttt gttggaccgt atttcagttg
    32701 agtactacgg ttctccaacg ccattgaacc agttggctgg tattacagtg ccagaagcgc
    32761 gtgttctttt gattactccg tttgataagt caatcttgaa ggatattgag cgtgctttga
    32821 acgcttcaga tcttggtttg acaccgcaat ctgacggtac tgttatccgt ttggttattc
    32881 ctgcccttac agaggaaact cgtaagaact tggcaaagga tgtgaaaaaa gtcggtgaaa
    32941 attcaaaagt tgccatccgt aatatccgtc gtgatgccat ggatgaagct aaaaaagctg
    33001 aaaaagcaaa agaaatcaca gaagacgagt tgaagacgct tgaaaaagat attcaaaaag
    33061 taacagatga tgctatcaag acaattgata aaatgactgc cgacaaagaa aaagaattgt
    33121 tggaagttta atcaacacgt ttttagacta caaaaataga tcatcactgg gctgtctaac
    33181 ttccagtggt gaaccaataa gaaatctaat ggggggagtt agacaagact tcccccactt
    33241 tttaatcaga aagaagaaaa tcatgaatga tttattagca cagtatatcg ttggtttagt
    33301 gactgatgaa aacgatcagt tttactttgt ccaaaaagag ggtagaacct ttgctctttc
    33361 taaggatgaa ggagaatata gtctaggtca atctgttaaa ggatttgcct ataccgatat
    33421 gaaacaaaaa ctccgtttga caactaagga agttggggcg agtcgtacca gttttggttg
    33481 gggaactgtc acagaggtac gtaaggattt gggtgtcttt gtggatacag gtctgccaga
    33541 caagcaagtg gttgtttcct tggacatttt gcctgaaatc aaggaactct ggccgaaaaa
    33601 aggggaacag ttgtacgtca aattagacgt cgataagaag gaccgcattt gggctctgcc
    33661 agcttttcaa gaagattttc agaaaatggc tggacctgct tatgacaata tgcaaaatca
    33721 aaatctgcga gcgattgtct atcgtttgaa aatgaatggt acttttgttt atttgccaga
    33781 caacaatatg cttggtttta tccatccgag cgaacgcttt gcggaaccac gtctgggtca
    33841 ggttttggag gcgcgtgtca tcggctaccg tgcagttgat cggaccttga acttgtctct
    33901 caagccacgt tcatttgaga tgctggaaaa tgatgctcag atgatattga cttatctgga
    33961 aagcaatgga ggtttcatga ccttgaacga taagtctgct ccggaagaca tcaaggccac
    34021 ctttggtatt tccaaagggc aattcaaaaa agctcttggt ggtttgatga aggctgggaa
    34081 aattaaacag gattcttttg gaacagagtt ggtctagggg gcgtctatgc tagtgtatat
    34141 tgctggaagt ggggcaatgg gttgtcgttt tggttatcaa ttgtctaaga caaatcatga
    34201 agtgattttg ctggataatt gggaagaaca tatcgaagcg attcgagaaa acggcttgaa
    34261 agtgactgga gacgtagagg aaactgtaca cttacctatc atgaaaccga ccgaagcaac
    34321 gagagaagca gattttatca ttctattgac aaaggctgat cagttgccaa agatgttgcg
    34381 ggatattaaa ccgattatcg gtccaactac caaggttttg agtctcttga atggtttggg
    34441 gcatgcgacg accatgcgtc ggtatgtgcc agatgaaagt attatggttg gggtgaccat
    34501 gtggttggcg ggactaaagg gtcctggaca tgctcactta gaaggtccgg gttctatctc
    34561 cgttcagcat attcttggtg atgagcaatc agttgcggat attattgaaa tgctgaatga
    34621 agctggacta aatgtgacct atgataatca tgttgtcagt gccatttggc agaaagcctg
    34681 tgtcaatggt gtgatgaatc caacttgtac cattttagac tgtacaattg gtgaattatt
    34741 tggtacggag gctgggctga atctagtaca ggggattttc aaagaattta ttgctgttgc
    34801 taagtctgaa gcagatggaa ttgacgaaga agctatttgg aaatatgtta ttgatgcttc
    34861 gaagaaggct tccaatcact atccatctat gcaccaggat ttggttcaac atggtcgaaa
    34921 gacagaaatt gattatctga atggagcggt tgtttttaag ggcaaacgtg caggtatccc
    34981 aacgccatac tgtcagatga taacagatat gattcatgcc aaggaaagta tgctggggtt
    35041 gagataggag aaagtcatgc tgattgaagt ggaaacgatt gaagaatata tagctgcctt
    35101 gcctgaaaat cggaaggaac cggttgaacg gctccatcaa gtgattgtgg aacagttacc
    35161 agctggtttt gaagtaggaa tcttgggagg tatgattaac tattatgtgc cgcttgctgc
    35221 ctaccctgat ggttatcatt gcacaccgag agagcccgtg ccattccttg ccttggcatc
    35281 acaaaaggca cacatcgccc tctaccacat gggaatctat atggaccaag aattgaatga
    35341 ttggttcgtt gctgcttatc aagcacaggt gcctaccaaa ctggatatgg gaaagtcctg
    35401 tgttcgtatg aaaaatccca agaatattcc ttatgaattg attggggaat tggttagtaa
    35461 gatgtctatg gagcgctata tagagctcta cgaaaaaaat catagaaagt agatgtagtg
    35521 agaagaagtt tttattcttg gttgatgacc cagcgcaacc cgaaaagtaa tgaaccagta
    35581 gctatcttgg cagattatgc ctttgatgaa tctgattttc ctaagcaatc agataatttc
    35641 gatgaagtca gtcgtttttt ggaggaatcg gccagttttg ctttttccat gtctgatttt
    35701 gatgtgattt gggaagatta tttaggacat tgatggataa aatagtacaa aatagccttg
    35761 tttgggctat ttttgttata atagagaaaa gcacaggaaa aggagcaact cttgctagaa
    35821 cattcaattg atattcaact caagcatcca gatgattcgt ttagtctatt tggttcgaat
    35881 gaacgccatc tgagattgat ggaacaggaa ctaggtgtgg ttattcatgc ccgtacggaa
    35941 aaagtgcagg tgattggaca ggaagaacat gtagaacagg cgcgtcttgt tatccagtct
    36001 ctccttgtct tggtgagtcg tggtttggtt atcaatacgc cagatgtggt cacagctatt
    36061 tccatggcaa aaaatgatga gattgagaaa ttcgttgccc tctatgaaga agagattatc
    36121 aaggacaact atggcaaacc cattcgcgta aagacccttg gacaaaaact ctacgtagac
    36181 agcgtaaagc gtcatgacat cgtgtttgga attggaccag ctggtacagg gaagaccttc
    36241 ttggcggtta ctctagctgt aacagccctc aagcggggac aggtcaagcg gattgtcctg
    36301 acccgtcctg cggtcgaggc aggcgaaagt ttaggctttt taccagggga cttaaaagaa
    36361 aaagtagacc cctatttgcg tcctgtctat gatgccctct accagatttt gggcaaggag
    36421 cagaccacgc gcctaatgga gcgcgagatt atcgagattg caccgctggc ctatatgcgg
    36481 gggcggacct tggaagatgc ctttgtcatc ttggatgagg cgcaaaatac gaccattatg
    36541 cagatgcaga tgttcctgac ccgtctgggc tttaattcca agatgattgt caatggggat
    36601 attagtcaga ttgacctgcc acgcaaggtt aagtcaggcc tgattgatgc gacagagaaa
    36661 ctaagcaaaa ttccacagat tgatttcgta catttttcag ccaaggatgt ggttcgccat
    36721 ccagttgttg cccagattat caatgcctat gaagaggagg cttgggcagc tgatagtcaa
    36781 gcaagttttg aaacgattgg ggagcctagt cggagcgaag agaatgaaga ttagtcaatt
    36841 ttcaagtcat tatcaagtac gaaagttagc agacgccgac ttagagcagg ttttagcact
    36901 ctaccagacc aatcccctct attttgaata tttccaccct tgcctagtct ggaaagttta
    36961 gcaaatgacc ttgtcgagtg tccgccaggt aaggagttat cagataaata ttttctagga
    37021 ttctgggaca aagagcgatt agttgccatc ttagacccaa ttgacggcta tccgactgca
    37081 gagattgcct atatcggact ttttatggtg gattcaagat tggctggtca gggtcttggt
    37141 tcaaaggtca tagcaaagct cttaactcag ttagcaactc acttcaaaaa ggtgcgtcta
    37201 ggctatgttg aaagcaatcc tcaggccagt catttttggt ccaaagttgg attgtgttca
    37261 actggcgagg ttaaagaaat agctggaaaa acaatcgtca tagccgaaca tgcattagat
    37321 gaataataat aaaaaaactg cctagttcaa tcagaactgg gcagtactta ttatgtgact
    37381 tttagtccgt cacgccccgt aaggtgcgtg acagataaac cacccgctat gcgggtgcgc
    37441 gacgaaggtt ataccaaaaa aactccaaac gcgatagaat aaaggtgttc aagcctattg
    37501 taaagcgaaa ggagaaaaat atgg
//
LOCUS       DATUCG010000006        37094 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.006, whole genome shotgun sequence.
ACCESSION   DATUCG010000006 DATUCG010000000
VERSION     DATUCG010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 37094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37094
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.006"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(217..1110)
                     /locus_tag="VCA20_000250"
     CDS             complement(217..1110)
                     /locus_tag="VCA20_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HEP1834122.1"
                     /translation="MLELKNENLTVQFSELGGQIISIKDKDGIEYLWQGDPTYWSGQA
                     PVLFPICGSLRNDWAIYEPAERPTFTGTIPRHGLVRKMMFKNVNSTENSLEFSISSNE
                     ETVKNYPFEFELSINYSLLGNTIRTEYRVKNLEGHRKLPYFIGGHPGFNCPLLDGESY
                     EDYYLEFEKVESCTVPRSFPETGLLDLRDRRPFLENQKILDLSYKLFEHDAITLDQLA
                     SRKVTLKSKNHQKKLSIAFDDFPYLVIWSTTNQSPFIALEPWSGLSTSLEESDIFNAK
                     RSISYVAPKEVDKKHFDIIIE"
     gene            complement(1175..2581)
                     /gene="lacG"
                     /locus_tag="VCA20_000251"
     CDS             complement(1175..2581)
                     /gene="lacG"
                     /locus_tag="VCA20_000251"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEP1834123.1"
                     /translation="MVKTLPKDFIFGGATAAYQAEGATKTDGKGRVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLELSEQFGVNGIRISIAWSRIFPTGFGEVNPKGVEYYHKLFAE
                     CHKRHVEPFVTLHHFDTPETLHSNGDFLNRENIEHFVNYAAFCFKEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHAKAVKLYKDAGYSGEIGVVHAL
                     PTKYPYDPSNPDDVRAAELEDIIHNKFILDATYLGYYSEKTLEGVRHILKVNGGELDL
                     RDEDFAALDAAKDLNDFLGINYYMSDWMRTHDGDTEIIHNGKGEKGSSKYQIKGVGRR
                     ESPVDIPKTDWDWIIYPQGLYDQIMRVKNDYPNYKKIYITENGLGYKDEFVDGTVYDD
                     GRIDYVKKHLEVISDAISDGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAYWYKKVAETQVIE"
     gene            complement(2816..4495)
                     /locus_tag="VCA20_000252"
     CDS             complement(2816..4495)
                     /locus_tag="VCA20_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HEP1834124.1"
                     /translation="MNKLIEFIEKGKPFFEKISRNPYLRAIRDGFIAAMPVILFSSIF
                     LLVAFVPNIFGFTWSDEAVAAIMKPYGYTMGIVAVLVAGTTAKSLTDAFNRQLPKTNQ
                     INFISTMIASISGFLLLASDGIEGGFANGYMGTKGLLTAFLAAFITVNIYKVCVKNNV
                     TIRMPDEVPPNVSQAFKDVIPYALSIFVLYGIDLVTRQFLGTNVAEAILKLFEPLFTA
                     ADGYVGITIIFGAYALFWFVGIHGPSIVEPAIAAITYANIETNFQLLQAGQHADKILT
                     SGTQMFIVTMGGTGATLVVPFMFMWLTKSKRNKAIGRASVVPTFFGVNEPILFGAPLV
                     LNPVFFVPFILAPIANVWIFKFFVDTLKMNSFSVNLPWTTPGPLGIVMGTNFAPLAFA
                     LAILLVFVDVLIYYPFLKVYDEQILAEEQSGKVENSLKEKVAANFNTAKADAILEKAA
                     VETEISEQTNVLVLCAGGGTSGLLANALTKAAKEYGVPVTATAGSYGAHREILPEYQL
                     VILAPQVASNYDDIKQETDALGIKLAKTEGAQYIKLTSDGQGALDFVKQQF"
     gene            complement(4495..4812)
                     /locus_tag="VCA20_000253"
     CDS             complement(4495..4812)
                     /locus_tag="VCA20_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEP1834125.1"
                     /translation="MNREEITLLGFEIVAFAGDARSRLMEALAAAQKGDYAKAEELVE
                     AANACIVEAHHAQTSLLQKEAAGEDLAFSVTLMHGQDHLMTTLLLKDMMSHMIELYKR
                     GDK"
     gene            complement(4943..5776)
                     /locus_tag="VCA20_000254"
     CDS             complement(4943..5776)
                     /locus_tag="VCA20_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEP1834126.1"
                     /translation="MFRIIQSLNNNAALVKNEHGEQAVVMGLGITFQKKKGDLIVKDK
                     IENIFSLKNDEAKENFLTLLKDIPLDFISATYTVINHLVETYHYPVQEYLYVTLTDHI
                     YCAYQAVLKNTYQESRLPNISAEYPLEYKMAREALAMFREKLLNDFPNDEIGKIALHL
                     INAKGETIHPTSEEHDISKKIIAEVEQILIDNGIRRSKENSNFYDRFMIHLSYFLNYL
                     DRSHQSNESIASLEQYIKLQNPRAHQVGSQIFEMITSLVDEPVNDSERFYIVLHIQRL
                     L"
     gene            complement(5969..6865)
                     /locus_tag="VCA20_000255"
     CDS             complement(5969..6865)
                     /locus_tag="VCA20_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006531659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HEP1834127.1"
                     /translation="MVIELKNDFLTVQFKEFGGALSSIKDKDGVEYLWQGNPEYWSSQ
                     TPVLFPICGSLRDDEAIYRPSSRPHFTGVIPRHGLVRKKTFTYEPLSENSLVFSITPD
                     AEMLANYPYDFELKIIYTLIGKTIKTSYQVTNRETEKVMPYFIGGHPGFNCPLLADEC
                     YEDYYLEFEKEETCTVPESFPETGLLDVNKRTPFLEKQNILNLDYALFAKDAITLDKL
                     ASRSVSLKSHKHEKGLRLDFEDFPNLILWSTVNKSPFIALEPWSGLSTSLDEGDDLED
                     KRQVTFVASGEMSEKTFGITIF"
     gene            complement(6975..8375)
                     /locus_tag="VCA20_000256"
     CDS             complement(6975..8375)
                     /locus_tag="VCA20_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002265221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol transporter subunit IIA"
                     /protein_id="HEP1834128.1"
                     /translation="MNVIIDFANTIFKPIIGLGAAPLMTIILTVIALFFKVKFTKALE
                     GGIKLGIALTGISAVINILSTAFSGAMADFVARTGLELNITDVGWAPLATITWGSPYT
                     LYFLMVMVIVNIIMLVLNKTNTLDVDIFDIWHLSIVGLFAIYSGANLMIATILVIFIG
                     ILKIINSDLMKPTFNDLLGTGDENPMTTTHMNYMMNPIIMVFDKIFDKLFPWLDKYDF
                     DAAKLNAKIGFWGSKFAIGIYLGIFVGLLAGQTPAEIASLAFTAAVCLELFSLIGSWF
                     IASVEPLSQGITDFASSKLQGRTLNIGLDWPFIAGRAEVWAAANILAPIMLLEAIVLP
                     GNKLLPLAGIIAMGVTPALLVVTRGKLIRMIVIGAIELPLFLWSGTLIAPFITETAKK
                     VGAFPSGLDASTMISHTTMEGPIEKFLAFFVGKASTGDLTYVLYAAIALVVYTLLFFW
                     YKGEMKKRNQAYQEAK"
     gene            complement(8411..8713)
                     /locus_tag="VCA20_000257"
     CDS             complement(8411..8713)
                     /locus_tag="VCA20_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003063077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HEP1834129.1"
                     /translation="MIKILAACGAGVNSSHQIKNALETELTKRGYQVSCDAVMIKDIN
                     EELLSHYDIFAQISKTDLGFDVKIPVVDAGAILYRIPAMAEPVYKKMEDVINSLNK"
     gene            complement(8727..9200)
                     /locus_tag="VCA20_000258"
     CDS             complement(8727..9200)
                     /locus_tag="VCA20_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002267762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEP1834130.1"
                     /translation="MTQLFAEDTVYISEQQDQKSVFKEIAHKLFEKGLVTEEYLDNLI
                     DREEHYPTALPLSPIDSSLPNIAIPHTESQFVNVTRIVPVKLEHAITFHNMILPDEKL
                     EVSFLFMILNNEEKEQAGLLAAIMDFINRQDKEALIKFFNSENPERIYQFLENNF"
     gene            complement(9213..10193)
                     /gene="lacD"
                     /locus_tag="VCA20_000259"
     CDS             complement(9213..10193)
                     /gene="lacD"
                     /locus_tag="VCA20_000259"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEP1834131.1"
                     /translation="MVVTADKRKYMEKVSNEAGIISALAFDQRGALKKMMAKHQVEEP
                     TVVQMEELKKLVSEELTQFSSSILLDPEFGLPASRARDEQCGLLLAYEKTGYDTSSTS
                     RLPDCLVEWSVKRLKEEGADAIKFLLYYDVDGDSYVNLQKQAYIERIGAECKAEDMPF
                     FLEILTYDENITDNTSAAFAKVKPHKVNEAMKVFSDDRFGIDVLKVEVPVNMKYVEGF
                     ADGEVVYTKEEAAKAFKDQEAASHLPYIYLSAGVSAKLFQETLVFAAESGAKFNGVLC
                     GRATWAGSVQVYIEEGEAAAREWLRTQGRKNIEELNAVLIKTASSWSEKV"
     gene            complement(10195..11127)
                     /locus_tag="VCA20_000260"
     CDS             complement(10195..11127)
                     /locus_tag="VCA20_000260"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEP1834132.1"
                     /translation="MILTITLNPSVDIAYQLDTFHLDTVNRVEKVQKTAGGKGLNVTR
                     VLKQIGEDVVATGFIGGEIGSYIKKQLTRNDIKNSFVEIGNETRNCIAVLHDGQQTEI
                     LEQGPTIQEHESLNFIEHLEIILNNIDVVVISGSLPKGLAGNYYVKIIELCKKYGVAV
                     VLDCSGEALKKVLESQQKPTVIKPNTEELSQLIGKEVTDDIQELKSVLSGQLFQGIDW
                     IVVSLGAQGAFAKHNDKFYRVRIPKIKVVNPVGSGDSTVAGIAAGLVHALPDAELLKN
                     ANVLGMLNAQEEQTGYVNLENAESLYSQIEVEEV"
     gene            complement(11137..11652)
                     /gene="lacB"
                     /locus_tag="VCA20_000261"
     CDS             complement(11137..11652)
                     /gene="lacB"
                     /locus_tag="VCA20_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEP1834133.1"
                     /translation="MKIAIGCDHIVTYEKIAVVDYLKNQGYEVLDFGTYDNVRTHYPI
                     FGKKVGEAVASGQADLGICICGTGVGINNAVNKVPGVRSALVRDMTSALYAKEELNAN
                     VIGFGGKITGELLMCDIIEAFIKAEYKPTEANKCLIEKIAAVETLNQEQANPEFFTEF
                     LEKWDRGEYHD"
     gene            complement(11679..12101)
                     /gene="lacA"
                     /locus_tag="VCA20_000262"
     CDS             complement(11679..12101)
                     /gene="lacA"
                     /locus_tag="VCA20_000262"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEP1834134.1"
                     /translation="MTIIIGADPAGSKLKDVIKDYLLEKSYDFKDVSDGSDFVDTTLA
                     VVAEVNQEEGNLGIVIDAYGVGPFMTATKVKGMIAAEVSDERSAYMTRGHNNSRIITI
                     GSEIVGEGIAKNIVKGFVEGEYDGGRHQIRVDMLNKMC"
     gene            complement(12307..13074)
                     /locus_tag="VCA20_000263"
     CDS             complement(12307..13074)
                     /locus_tag="VCA20_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEP1834135.1"
                     /translation="MNKRERLEEITRLVNKKGTVRISEIVELLNVTDMTVRRDFIELE
                     EQGVLTKIHGGARSNKAFQYREYSHSEKHIQNKEAKQEIAERAAQLIEDGDTVFLGPG
                     TTVAFLAEAIKNPRLTVITNCMPVFTILSQKKTEEFQVFLLGGEYRQVTESFVGEITN
                     TSLEKMRFSKIFFSGNGLKGTDVMTSTLAEAYTQNIAIKHSLEKYLLLDASKIGKDDF
                     TSFCDLNDVTALVTDMNAKDENYQLLSKHIEIISTKS"
     gene            13353..14108
                     /locus_tag="VCA20_000264"
     CDS             13353..14108
                     /locus_tag="VCA20_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834136.1"
                     /translation="MALGNVEKDTEGWIELVNQYLQYCIEIGLSPYTQATYKVALAKV
                     LGVSSTNFIATQPRTRANRMNNRVLHKDYRLSNKNNDYWHKVVTATGLRKSELIHVTG
                     DALQRGRDGRWYLNLAGHKHHTKERRDRWSPIMATSQEEEEWLVAIFQRAGEKKVFHV
                     PKDLILDDFDGKKVPTALKSHKYPAEYAERVYRSVAREISKIRNRKEVIHLRKELVDI
                     SLDRKACKIVTKALGHNRPEEFPRSYAYILLKR"
     gene            14476..15523
                     /locus_tag="VCA20_000265"
                     /pseudo
     CDS             14476..15523
                     /locus_tag="VCA20_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(15749..16291)
                     /locus_tag="VCA20_000266"
                     /pseudo
     CDS             complement(15749..16291)
                     /locus_tag="VCA20_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16311..16781)
                     /locus_tag="VCA20_000267"
     CDS             complement(16311..16781)
                     /locus_tag="VCA20_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834137.1"
                     /translation="MKRITEISWNDIYKEWETYANHFGLTTPINTEKLRDQKSKDFGK
                     GSLITLDLLADYDTDSEKTAAIWVASFCRDLIQDYAYLLNGIAYLTVNQIYFQAVKQF
                     QSEAVIWSKPLTRLQPKLFVSYRLLENLDLSHYSCVVELAMLQASMVRTQILEK"
     gene            16913..17713
                     /locus_tag="VCA20_000268"
     CDS             16913..17713
                     /locus_tag="VCA20_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP1834138.1"
                     /translation="MILGDILKEYRSKNKLSMDKFAELSGLTKGYISMLEKNQHPKTK
                     KALLPTMDTLEKVAKGMAISVGELIEQLDSNQDIALTITPSQFKLFQQPIHPEVQTLD
                     NELKGDFHAQWLTFGQEQLAAMQTASVTPSASILHLDDYREQSELPVPGKVSAGTGYW
                     QDTDMNNLVSFYNDEIPDNQSYDTIAQVVGDSMAPHIEDGDYLFIRLTPHIPLNTIGI
                     FSVNGENFVKKFKGDYLQSLNSDYDDIYFSPEDDIRPIGQVVDIHSPD"
     gene            complement(18376..19536)
                     /gene="mutY"
                     /locus_tag="VCA20_000269"
     CDS             complement(18376..19536)
                     /gene="mutY"
                     /locus_tag="VCA20_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEP1834139.1"
                     /translation="MLNLKEYGIEMWPEEKIQAFRKALLDWYDANKRDLPWRRTKDPY
                     AIWVSEIMLQQTRVDTVIPYYERFLHHLPTISDLAQAPEEVILKLWEGLGYYSRVRNM
                     QKAAQQMVEDFDGQFPTTHAAISSLKGIGPYTAGAISSIAFNLPEPAVDGNVMRVLSR
                     LFEVDYDIGLPANRKIFQAMMELLIDPERPGNFNQALMDLGADIESPVNPRPQDSPVK
                     AFSAAYLNGTMDKYPIKAPKKKPIPVAYQGFLIRNKDNQFLLEKNNEAGLLSGFWSFP
                     LLEKGAIVEKQVSLFEVAEEVVQPDIRQSFTELYGLTVDWQEQEFGIVQHIFSHRKWQ
                     IEMVEGVAETLNLPESKELKWVSVEDFPTYPFAKPQQKMWENFIKEKEKQKS"
     gene            complement(19770..20162)
                     /locus_tag="VCA20_000270"
     CDS             complement(19770..20162)
                     /locus_tag="VCA20_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEP1834140.1"
                     /translation="MKVLDKLNLQPITVLDDYQATMLNEKEVLLTTKVTEKSLNPYGM
                     AHGGFLFTLADSVAGLTTVARGSYSVTLQSNIHYMKAAKLGDTLSVIGSCTHDGSRTK
                     VVEVKIENQDKQLLASASFTMFVTGKVE"
     gene            complement(20146..21888)
                     /locus_tag="VCA20_000271"
     CDS             complement(20146..21888)
                     /locus_tag="VCA20_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1834141.1"
                     /translation="MRQSSFKELVRLVLHQPLRMSLMLVGTLVQVLLTVYLPILIGQA
                     VDAVLIANGQVLLGILGKMVMVILLNTLVQWYLPLVTNRLVYGMVADLREQVYIKLHQ
                     MPLSYLDRHSVGDLVARFSSDSEQLTNGLLMIFNQFFIGILTIFLTIFTMARLDLMMM
                     LVVVALTPVSLLVARYIAKKSYGYYRQQTQARGRQSQLLEESISQLTLVQSFNAQEQF
                     TQGFQVVNDQYATYSQQAIFASSTVNPSTRFINAIIYALLAGLGALRIMAGQFTVGSL
                     TTFLNYASQYSKPFNDISSVLSELQSALACADRLFAILALDSIDDQAERKIDSEELKG
                     AISFDNVSFSYSADRSLIEHLDIDVKAGQKVAIVGPTGAGKSTMINLLMRFYDVTAGQ
                     IVLDGVPISQYSREDLRRQIGMVLQETWIKSGTIHDNIAYGYPNATRELVIEAAKAAN
                     ADFFIRQLPQGYDTVLADGGEGLSQGQRQLLSIARVFVKIPKILILDEATSSIDTRTE
                     ILVQEAFAKLMEGRTSFIIAHRLSTIQSADLILVMVDGKIVEQGNHEALMAEQGVYYQ
                     MQTSQQTEEDESVR"
     gene            complement(21875..23614)
                     /locus_tag="VCA20_000272"
     CDS             complement(21875..23614)
                     /locus_tag="VCA20_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1834142.1"
                     /translation="MKFLSRYFKDYIKESILGPVFKLLEACFELLVPLIIAYIVDTII
                     PNGSQGNLVAMLFLLVGIACIGIIVSLIAQYYSAKAAVGVTKELTNDLYQKVLSLPKS
                     SRDILSSSSLLTRLTSDTLQIQTGINTFLRLFLRAPIVVFGSLIMAFYISPSLSAYFL
                     GMIILLIFIVTVISVMTSRIYQLMRKELDGLVGQVRETVTGWRVIRAFGQREREIKAF
                     QGINQIYKKQQLQAGFWSSLLSPLTFLVVNGTLLILIWQGNIAISHNLLEQGMLVALI
                     NYLLQILVELVKMIMVVSTLNQTYISAQRIQEVFDQTSEDVESSLPKVVSEDKEIIFS
                     VRHLSFSYPKSAEESLSDIAFDLRKGQFMGIIGGTGSGKSTLVDLLQALYSVPTNQLS
                     LFIDGKSPKNLKEWRQRIAIVPQQAQLFAGTIRSNLSLGLEEISDSDLWSALEIAQAK
                     SFIEDKGGLDSPVEAFGKNFSGGQRQRLTIARAILQKAPILILDDATSALDYLTESRL
                     LVAIRQELPGQNLIMVSQRTNSLRTADQILVLEQGRQVGLGRHEDLLRSSAIYQEIHQ
                     SQQQGEEDSYETI"
     gene            complement(23616..24590)
                     /gene="pta"
                     /locus_tag="VCA20_000273"
     CDS             complement(23616..24590)
                     /gene="pta"
                     /locus_tag="VCA20_000273"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEP1834143.1"
                     /translation="MKIFEQLATKLEGKKVRIVLPEGEEPRILQATKRLVNESDVVPV
                     LLGNPDRIKIYLDIEGITKGFEIIDPAHYDRYDEMVAALVERRKGKVSEEQAHELLKD
                     VNYFGVMLVYMGVVEGMVSGAIHSTASTVRPALQIIKTEPGVSKTSGAFLMVKGEERY
                     IFGDCAINIDPDAQTLAEIAINSARTAKMFGIDPKVAMLSYSTKGSGAGPKVDKVVEA
                     TKIAQKLRPELDLDGELQFDAAFVPTTGKLKAPGSSVAGQATVFIFPGISAGNIGYKI
                     AERMGGFAAVGPVLQGLNQPVNDLSRGCNPDDVYNLTLITAVQALEHR"
     gene            complement(24608..25501)
                     /locus_tag="VCA20_000274"
     CDS             complement(24608..25501)
                     /locus_tag="VCA20_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEP1834144.1"
                     /translation="MRFEFIADQHTKIKTFLKKHGVSKGLLAKIKYTGGNIWVNDIEQ
                     NATYLLDIGDSVTIDIPAEEDLTGSLKPIFFPLDIVYEDDHFLAINKPVGYASIPSAL
                     HSSTIANFVKGYLIEQNYENKQVHIVTRLDRDTSGVMLFAKHGYAHARLDKQLQAKLI
                     HKRYYALVKGNGQLEETGDIIAPIGRPEDSIITRCVTKNGKYAHTSYRVVQSWGDIHL
                     VDIQLHTGRTHQIRVHFSHIGFPLLGDDMYGGSLECGIERQALHCHNLAFDNPFSAER
                     IDLEAPLPEDFQAVINQLISK"
     gene            complement(25501..26328)
                     /locus_tag="VCA20_000275"
     CDS             complement(25501..26328)
                     /locus_tag="VCA20_000275"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002933692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEP1834145.1"
                     /translation="MIQMKNTGRKKIALLASRNPKSEAVSKELWTKLKEANFILTPKN
                     PDIVISIGGDGMLLSAFHKYEKLIDRVRFVGIHTGHLGFYTDYRDFEVAKLIENLKLD
                     TGARVSYPILNVKVKMTDGRIVEARALNEATVKRLSKTMVADIIINNVPFERFRGDGI
                     SVSTPTGSTAYNKSLGGAVLHPTIEALQIAEVASLNNRVYRTLGSSVVVPKKDKIVIE
                     PKHSDRYSIAVDNKTFVYDSIESIEYQIDNSKIHFVATPSHTSFWNRVKDAFIGEVE"
     gene            complement(26303..26974)
                     /locus_tag="VCA20_000276"
     CDS             complement(26303..26974)
                     /locus_tag="VCA20_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEP1834146.1"
                     /translation="MDFNWEEFLDPYIQTVGELKIKLRGIRKQYRKANRHSPIEFVTG
                     RVKPIESIKEKMALRHIKLENLAQDMQDIAGLRLMVQFVDDIEEVLAILRKRKDMQIV
                     HERDYINNMKASGYRSYHVVIEYPVDTINGNETVLAEIQIRTLSMNFWATIEHSLNYK
                     YKGNFPEEIKKRLEVTAKIAYELDEEMRKIRNDIQEAQALFDPAYRKLNDGVGNSDDT
                     DEEYR"
     gene            27061..27627
                     /locus_tag="VCA20_000277"
     CDS             27061..27627
                     /locus_tag="VCA20_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEP1834147.1"
                     /translation="MKNHLEIEYKTLLTKSEYKRLLTDFSDVSPILQTNHYIDTPDLD
                     MKNHRFSLRIRTFEDSAELTLKIPQEVGNQEYNQVLDSQTAHDLLENFQLPAGQISDI
                     ISATEIPLDKLAVWGSLTTKRYEKQTSIGLMALDENDYVGQKDFELEVEVTDADAGKI
                     LFDEFLKKKSIQFKYASSKVARTAAHMK"
     gene            27772..28749
                     /locus_tag="VCA20_000278"
     CDS             27772..28749
                     /locus_tag="VCA20_000278"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEP1834148.1"
                     /translation="MVQPLGEHNIKLFSLNSNRDIAEKIADSAGIPLGKLSSRQFSDG
                     EIQINIEESVRGVDVYIIQSTSYPVNNHLWELLIMIDACKRASANTITAVIPYFGYAR
                     QDRTASPREPITAKLVANMLVKAGVDRVLTLDLHASQIQGFFDIPVDNLLTEPLFAAY
                     YMEKGLCGEDVVIVSPKNSGIKHARNIAEFLNAPIAIIDYVQDDSKRSEGYIIGDVAG
                     KKAILVDDILNTGRTFSQASKIVQDGGATEIYAVASHGLFAGTAAQLLDEAPIKEILV
                     TDSVASKEQHPKNIAFLTASDLIADAIHRIQEHQPLSPLFKFTSPEKEN"
     gene            28760..29875
                     /locus_tag="VCA20_000279"
     CDS             28760..29875
                     /locus_tag="VCA20_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEP1834149.1"
                     /translation="MIYLDNAATTALSPTALQRMMEVAQENYGNPSSIHQSGRKANQC
                     LRQSRQEIAQILYASPEQIIFTSGGSEADTLAIQGYALAHQSKGKHLITTAIEHHAVL
                     HTVQYLEERFGFEVTYIQPVDQVITAQQIQDALRPDTILVSVMFANNETGQLLPIKEI
                     GELLADHQAVFHVDAVQTIGKIAISPSDYGIDLLAASAHKFHGPKGTGFLYSKVHKFD
                     SLIHGGKQEQQHRAGTENLPAIAAMATALTEQYESLDTHHQHVSELRQHIIEGLSGLD
                     YYLNQAGPHLPHVLNIGFPGKLNEQLLMQLDLAGIAVSSGSACTAGVVQNSHVLEAMY
                     GKDSHRLKESIRISLSEINTKEEIDTLIIELKKILGG"
     gene            29877..30221
                     /locus_tag="VCA20_000280"
     CDS             29877..30221
                     /locus_tag="VCA20_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEP1834150.1"
                     /translation="MAFQQSVTLKDCNFTYHISPAIKKYTLRDNTFEQTKAGHYQLTR
                     LLEEIPNSNQGFLLKIIINQDLTGFKINITDKSGLHLVNIFKPESNKVIQEKFYFLMD
                     SLIDRDIFTKEA"
     gene            30223..30426
                     /locus_tag="VCA20_000281"
     CDS             30223..30426
                     /locus_tag="VCA20_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEP1834151.1"
                     /translation="MIEIHYLDAYKQERIQTFENKDAAILAFSGCLTLPDYYPVTSIT
                     QDGQALDYKGTIGDLYRYLQTLD"
     gene            30561..31199
                     /locus_tag="VCA20_000282"
     CDS             30561..31199
                     /locus_tag="VCA20_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEP1834152.1"
                     /translation="MKNDKKSDIPRATAKRLSLYYRMFKRFYAGNIEKASSKEIAEAI
                     GIDSATVRRDFSYFGELGRRGFGYNVKELMDFFADILNDTSITNVMLVGVGNMGRALL
                     HYRFHERNKMKIVMAFEADDNPAVGTTDENIPIHAISEIKERISEANSQTAILTVPSV
                     KAQEVTDILVEAGVKGILSFSPVNLSVPKDVVVQYVDLTSELQTLLYFMRKG"
     gene            31203..31895
                     /locus_tag="VCA20_000283"
     CDS             31203..31895
                     /locus_tag="VCA20_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEP1834153.1"
                     /translation="MKKPVIGISGNEYKTGDHTEPLLSYTQTCLVQAIEDAGGLPLIL
                     PVIEPDLAKYYIHLIDKLILTGGQNVQPSYYHEERTIDSDNYLPKRDEFELALIRAAQ
                     ENQKPIFGICRGLQLYNVAQGGSLHQSISEHWQDIDGQEVSQTIQLTQNSLLYDIYKS
                     DPSVNSFHRQAIKDLAPDLEIIALSDNQQIIEAVHSAYPTKFLGVQWHPELLYGKREI
                     EKELFHYIVNKL"
     gene            complement(31892..32572)
                     /gene="radC"
                     /locus_tag="VCA20_000284"
     CDS             complement(31892..32572)
                     /gene="radC"
                     /locus_tag="VCA20_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEP1834154.1"
                     /translation="MYQIEFKEEAILPRERLVEVGAERLSNQELLAIFIRTGTKKEPV
                     SILSNKLLNRLESLAALRELSIEELQSLTGIGRVKAIEIKAMIELGKRINQSELLLNE
                     RILGSEKLGRKMIHEIGHKKQEHLVALYLNTQNQIISQKTIFIGSVNRSIAEPREILH
                     YAVKCMATSIIIVHNHPSGSVQPSRNDLLFTENLKESCEKLGLVLLDHLIVGNKDYYS
                     FREESELF"
     gene            complement(32686..33435)
                     /locus_tag="VCA20_000285"
     CDS             complement(32686..33435)
                     /locus_tag="VCA20_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HEP1834155.1"
                     /translation="MSKREKKKKRKGSFWRNFLAVVLILISLALIFNTSIRNFIIGWN
                     TNKYQISNVTTEDIEKNKQAETTFDFDQVQSISTEAILAAQWDAQRLPVIGGIAVPEL
                     GINLPIFKGVFNTSLMYGAGTMKENQEMGKGNYALASHNIFGVTGAADVLFSPLDRAK
                     NGMKIYITDKTNVYTYVIDSVEIVSPESVYVIDDVEGRTEVTLVTCTDYNATQRIIVK
                     GVLESTTPYNETANDILDSFNKSYNQYDYGQ"
     gene            complement(33442..35886)
                     /gene="gyrA"
                     /locus_tag="VCA20_000286"
     CDS             complement(33442..35886)
                     /gene="gyrA"
                     /locus_tag="VCA20_000286"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEP1834156.1"
                     /translation="MQDKNLVTVNLTNEMKSSFIDYAMSVIVSRALPDVRDGLKPVHR
                     RILYGMNELGITPDKPHKKSARITGDVMGKYHPHGDSAIYEAMVRMAQWWSYRHMLVD
                     GHGNFGSMDGDGAAAQRYTEARMSKIALEMLRDINKNTVNFADNYDASEREPEVLPAR
                     FPNLLVNGTTGIAVGMATNIPPHNLGETIEAVKLVMDNPEVTTREIMEVLPGPDFPTG
                     ALVMGKSGIHRAYETGKGSIVLRSRTEIEEYGNGRERIVVTEFPYMVNKSKVQEHIVK
                     LVQEKRIEGITAVRDESNREGVRFVIEVRRDASANVILNNLFKQTQLQTNFSFNMLAI
                     QNSVPKILSVRQILESYIEHQKEVVTRRTRFDKEKAEARAHILEGLLIALDHIDEVIR
                     IIRNSETDAIAQAELMGKFDLSERQSQAILDMRLRRLTGLERDKIQSEYDELVALIAD
                     LADILAKPDRVVQIIKDELEEVKRKYADPRRTELMVGEVLSLEDEDLIEETDVLITLS
                     NQGYIKRLAQDEFQAQKRGGRGVQGTGVKDDDFVRELVSTSTHDRLLFFTNKGRVYRL
                     KGYEIPEYGRTAKGLPVVNLLKLEENEAIQTIINVTKDLDADSYLFFATRQGVVKRTS
                     VSEFANIRQSGLKALNLKKDDELINVFLTNGQADIIMGTKFGYSIRFTETDVRNMGRT
                     ATGVRGINLREGDQLVGATMISDDQEVLVLTEKGFGKRTPASEYPTKGRGGKGIKTLK
                     VADKNGSLAGLTTVSGDEDIMVITDTGVIIRTSVANISQTGRSTMGVKVMRLNDEAKI
                     MTFTLVDAAEIKEEKE"
     gene            36075..37058
                     /locus_tag="VCA20_000287"
     CDS             36075..37058
                     /locus_tag="VCA20_000287"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEP1834157.1"
                     /translation="MTATKQHKKVILVGDGAVGSAYAYALVNQGIGQELGIIDINKDR
                     TQGDAEDLSHALAFTFPKKIYSAEYSDAHDADLVVLTAGLPQKPGETRLELVEKNLRI
                     NQQIVTEIVNSGFNGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQAL
                     AEKIGIDARSVHAYIMGEHGDSEFAVWSHANVAGVKLYDWLQDNRDIDEQGLVDLFVS
                     VRDAAYSIINKKGATYYGIGVALARITKAIFDDENAVLPLSVYQAGQYEGVEDVFIGQ
                     PAIIGAHGIVRPVNIPLSEAELQKMQASAKQLKDIIDDAFANPEIAAGVKN"
BASE COUNT        11452 a         7926 c         6459 g        11257 t
ORIGIN      
        1 gattttggat ttcaagtagc tgttttcttc tcaaaaaata acaaaatcaa aacgctccat
       61 atccactttg attggtaggt ggctattttt gattttaaat gagtattaat ttaatttttc
      121 ataatattaa attaagcaat aaaccccact gacttattca gccagtgggg ttttgagtgt
      181 tttccgtaga gtatttaaat tacttaacta tggtatttac tcaataataa tatcaaaatg
      241 ttttttatca acttcttttg gagcaacata gctaatactt cgtttggcat tgaatatgtc
      301 agattcttcc agtgatgtag agagtccgct ccaaggctct aatgcaataa agggactctg
      361 atttgtagtt gaccaaatca ctagatatgg aaaatcatca aatgcaatcg aaagtttctt
      421 ctgatgattt tttgatttca aggttacctt tcgtgatgct aattgatcca atgtgatggc
      481 atcatgctca aacagtttat aggataagtc caagattttt tgattttcca agaatggccg
      541 gcggtctcgc agatcaagta agccagtttc tggaaaactt ctaggtactg tacaagattc
      601 tactttttca aattctaaat aatagtcttc ataactctca ccatccaaca agggacaatt
      661 aaaacctggg tgacccccaa taaagtaagg tagctttcta tgtccctcaa ggtttttcac
      721 ccgatattca gttcgaattg tattgccaag tagtgagtag ttaatggaaa gttcaaattc
      781 aaaagggtaa ttttttacgg tttcttcgtt tgatgaaata gaaaattcca aagagttctc
      841 tgtactattt acatttttga acatcatttt tctcacaagt ccatgccttg gaattgtgcc
      901 tgtaaatgtc ggtctttctg caggttcata gatagcccaa tcatttcgta aacttccaca
      961 aatcggaaat agaactggtg cttgaccgct ccaataggtc ggatcgcctt gccaaagata
     1021 ttcgatacca tccttgtctt taatggaaat aatttgccca cctaattcag aaaattgtac
     1081 tgttaagttc tcatttttta attcgagcat aaaaactgcc tcccttatat gaaaacaagg
     1141 atgctaactt gagctaggca tccttgtatt agttttattc aataacctgt gtttctgcta
     1201 cttttttata ccaataagca cttttctttg gataacgttc ttgggtctca aagtcaacat
     1261 agaacaaacc atatcgtttc tcgtatccat ttgaccaaga aaatacatcc atcaaggacc
     1321 agatgaaata acctttaaca ttggcaccat ctgagatagc atctgaaatc acttctaaat
     1381 gctttttcac gtagtcgatt cgaccatcat catagactgt accatctaca aattcatctt
     1441 tatagcctag accattttca gtgatgtaga ttttcttata atttggatag tcatttttta
     1501 cacgcatgat ttgatcataa agaccttgcg gatagataat ccaatcccag tccgtttttg
     1561 gaatatcaac tggagactct ctacgaccga ctcctttgat ttgatactta gaactgccct
     1621 tttcaccttt accattatgg ataatttcag tatcaccgtc atgagtcctc atccaatcac
     1681 tcatatagta attgatgccc aagaagtcgt tcaaatcttt tgcggcatct aaggcagcga
     1741 agtcttcatc acgaaggtct aattccccgc cattcacttt cagaatatgt cgaacgcctt
     1801 caagagtttt ttcagaatag taacccaagt atgtagcatc gaggataaat ttattatgaa
     1861 taatatcttc tagttccgct gcacgaacat cgtcagggtt gcttggatca taaggatatt
     1921 tcgttggcaa tgcgtgtaca acaccaattt ctccactata accagcatcc ttgtagagct
     1981 taactgcctt ggcatgtgaa accatcatat tgtggtgaga ttggaatacc ttagctaagt
     2041 catactgaat ccctggtggg aacttcccaa ctagatattg cccatcacca attggaccga
     2101 tttcattaaa ggttgtccag taattaactt cggggaattc tttgaagcaa aacgctgcgt
     2161 aattgacgaa gtgctcaata ttttctcgat ttagaaagtc accattagaa tgtagcgttt
     2221 ctggtgtatc aaaatggtga agcgttacaa atggttctac gtgacgttta tggcattccg
     2281 caaataattt atggtaatac tctactccct ttggattcac ttctccaaag cctgttggga
     2341 agatacgaga ccacgcaatt gaaatccgaa tgccattcac accaaactgt tcacttagtt
     2401 ctaaatcaac tggataacga ttataaaaat cagaagctgg ttccgcagtg taccagtaat
     2461 tatcttctag atacttgtcc cacgcaacac gaccttttcc atctgtcttt gtggcaccct
     2521 ctgcttgata tgcagcagtt gctccaccaa atataaaatc tttcggtaat gttttaacca
     2581 tatgcctacc ttcttctatc aaaaataaat aaaggggtag gagttggaga tgataactcc
     2641 ctcccctttt gattaagaac ctgtattcac aagtgaagtg cttgtatata agagatagtt
     2701 tttcgctaat caaggcgttt ttttgaggga atactgactg tattttgaaa aaaactaacg
     2761 atgagtagct gaaaaactag ccttagatgg aagtgctgaa ccgtgaatat aggttctaaa
     2821 actgttgttt gacaaagtca agagcacctt gaccatcact agtgagttta atgtattgag
     2881 ccccttcagt tttggcgagt ttaataccca atgcatcggt ttcttgtttt atatcatcat
     2941 agttcgatgc tacttgtggt gcaaggatga ctaattgata ctctggcaaa atctcacggt
     3001 gcgccccata gcttcctgct gtagctgtaa ctggaacacc atattctttt gcagctttag
     3061 ttagagcgtt ggccagcaag ccacttgtgc caccacctgc acaaagtacc agaacgttgg
     3121 tttgttcaga aatttcggtt tcgactgcag ctttttcaag gatagcatcc gccttggcag
     3181 tattaaaatt agctgccact ttctctttca agctattttc aactttccct gattgttctt
     3241 cagcgagaat ctgctcatcg taaactttca agaatggata atagataagt acatcgacaa
     3301 ataccaacaa gatagctagt gcaaaggcaa gtggagcaaa gtttgttccc attacaatcc
     3361 ccaatggtcc tggagttgtc catggcaagt taacgctgaa actgttcatt tttagcgtat
     3421 caacaaagaa tttgaaaatc catacgttag caattggagc taagatgaat ggaacaaaga
     3481 atactgggtt gagtacaagt ggcgcaccga acaagatcgg ttcgtttaca ccaaagaagg
     3541 ttggaacaac tgaagcacgt ccaattgctt tatttcgttt agacttagtc aaccacataa
     3601 acatgaatgg cacaaccaag gtagcacctg taccacccat tgtcacgata aacatttgag
     3661 tacctgaagt caggattttg tccgcatgtt gaccggcttg taaaagctgg aagttggttt
     3721 caatattagc ataagtaatg gctgcaatcg ctggttccac aatagatgga ccatgaatcc
     3781 ctacaaacca gaacaaagca taggcaccaa agataattgt aataccaaca taaccatctg
     3841 cggctgtaaa tagaggctca aataatttca gaatcgcctc agcaacgttt gttccaagga
     3901 attgacgtgt cacaagatca attccataca agacaaagat agacaatgca tatggaatca
     3961 catctttaaa tgcctgggat acgttcggtg gaacttcgtc tggcatacga atggtgacat
     4021 tgtttttcac acagacctta tagatattaa ccgtaatgaa ggctgctaga aaggctgtca
     4081 aaagtccctt agttcccatg taaccattgg caaatccacc ttcaatacca tcagaagcca
     4141 gtaataagaa acctgaaatt gaggcaatca tggttgaaat gaagttaatc tgattggttt
     4201 ttggcaattg acggttaaac gcatctgtca aagattttgc ggtcgttcct gcaactaaga
     4261 cagctacaat acccattgta taaccgtaag gtttcatgat agccgcaact gcttcatcag
     4321 accaagtaaa tccaaagata ttaggcacga aagctaccaa tagaaagata cttgagaaca
     4381 agataaccgg catggcagcg ataaaaccat cacggatagc ccttaaataa gggtttctcg
     4441 aaatcttttc aaagaaaggc ttccctttct caataaattc aatcaattta ttcattactt
     4501 atcacctcgt ttgtacagtt caatcatatg actcatcatg tctttcaata ataatgtggt
     4561 catgagatgg tcttggccat gcatgagcgt cacactaaag gctaaatcct caccagccgc
     4621 ttctttctgc aacaaacttg tctgagcatg gtgggcttca acaatacaag cattggcagc
     4681 ttcaactagt tcttctgctt ttgcgtaatc acctttttga gcagcagcca gtgcctccat
     4741 caaacgagaa cgagcatcac cagcgaatgc aacaatttca aaacctaata aagtaatttc
     4801 ttctctgttc ataacatgaa cctcctattt tttatgtact caatggaaat accattcaga
     4861 ctaggcgacg aaaacgtaga tagaactaaa gttcatcaag ttgaggcaac aacgtctgaa
     4921 ttggatttac gaagagtatg atttacaaca accgttgaat atgtaacaca atatagaacc
     4981 tttcactgtc attgacaggc tcatcaacca atgatgtaat catctcaaaa atctgactcc
     5041 caacctgatg ggctcttgga ttctggagct tgatatactg ttccaaacta gcaatcgatt
     5101 catttgactg atgggatcta tctaagtaat tcagaaaata ggacaaatga atcataaagc
     5161 gatcataaaa attgctattt tcttttgagc gtctaatccc attatctatc aaaatttgtt
     5221 caacctcagc aataattttc ttactgatgt catgttcttc acttgtggga tgaattgttt
     5281 caccttttgc attgattaag tgaagtgcaa ttttaccaat ctcatcattt ggaaaatcat
     5341 ttagcaactt ttcacgaaac atagctaagg cttcacgcgc cattttatat tccaatggat
     5401 attcggctga aatattgggt agtctacttt cttgataggt attctttaaa accgcctgat
     5461 atgcacaata gatatgatct gtcagtgtca catatagata ttcttgtaca gggtaatgat
     5521 aagtttctac cagatgatta ataaccgtgt aggttgccga aataaaatct aagggaatat
     5581 cttttaataa ggttagaaaa ttttctttgg cttcatcatt ttttagcgaa aagatatttt
     5641 ctatcttgtc cttaacaatt aaatcccctt tcttcttttg aaaggttata cctaatccca
     5701 tcacgactgc ctgttcccca tgttcatttt tcaccagggc agcgttatta tttagtgact
     5761 gtataatcct aaacatatat ccctcctata tttttaagca aaaaagacta caaacaaacc
     5821 gagaatatcc tactctcaaa tccatttgta gtcttgccta attatcttag ttacaatcca
     5881 ctctattaat ttctacagtt ataatataac acacaatttc gattttgtaa accctttctt
     5941 tgtgtttttt tgtttgtttt aaacattttt aaaaaattgt aattccaaag gttttctcac
     6001 tcatttcgcc gctcgctaca aaggttactt gacgtttatc ttctaaatca tcaccttcat
     6061 caagtgacgt tgacaagccg ctccacggct ctagagcaat aaaagggctc ttattgacgg
     6121 ttgaccataa aatcaaattt ggaaaatctt caaaatccaa acgcaatcct ttttcgtgtt
     6181 tatgagattt caaagaaacg ctgcgtgaag ctaatttatc caaagtaatg gcatctttgg
     6241 caaacaaagc ataatccaaa tttagaatat tttgcttttc taaaaatggc gtacgcttat
     6301 tcacatcaag aagccctgtt tctggaaaag attctggaac agtacaagtt tcctcttttt
     6361 caaattcgag ataataatct tcataacact catcagcaag caatgggcaa ttaaacccag
     6421 ggtgcccacc aataaagtaa ggcatgacct tttccgtctc gcgatttgtc acttggtaac
     6481 ttgttttaat cgtctttcca atcaaagtgt aaatgatttt caactcaaaa tcataaggat
     6541 aatttgccaa catttccgca tcaggggtga ttgaaaaaac aaggctattt tcagataatg
     6601 gttcataagt aaaagtcttc ttacgcacca aaccatgtcg cggtatcaca cctgtaaaat
     6661 ggggacggct actaggacga taaatagctt catcatcacg taagctccca caaatcggaa
     6721 ataatactgg tgtttgtgaa ctccaatact ctggatttcc ttgccacaaa tattcgacgc
     6781 cgtctttatc ttttatggat gacaaagcgc caccaaattc cttaaattga actgttaaga
     6841 aatcattttt taattcaata accattactt tcccctaaat aaaatgagaa tgaacagcct
     6901 cataactgcc attctcatca cgcgtaaaaa caaaataata aaagaagttt tagtgagtta
     6961 gattagatgt attactattt ggcttcttga taagcttgat tacgtttttt catttctcct
     7021 ttataccaga agaagagcaa cgtgtaaaca actaaagcaa tagctgcata aagaacgtaa
     7081 gtcaaatcac cagttgaagc tttaccaaca aagaatgcca agaatttttc aattggtcct
     7141 tccattgttg tatgagaaat catggtcgaa gcatctaaac cacttggaaa ggcacccact
     7201 tttttagcag tttctgtgat aaatggtgca atcaacgttc ctgaccacaa gaatagcggt
     7261 aattcaatag caccaatgac aatcatgcgg attaatttac cacgtgttac aactagcaaa
     7321 gctggagtaa cccccatcgc aataatacct gcgagcggta ataatttatt tcctggtaaa
     7381 acaattgctt ctaacaacat aattggagct aaaatattag cggcagccca tacttctgca
     7441 cgacctgcaa tgaatggcca gtccaaacca atatttaatg tgcgtccttg taattttgaa
     7501 gaagcaaagt cagtaatacc ttgagaaagt ggttcaacag aagcgataaa ccatgaccca
     7561 atcaatgaga acaattccaa gcagacagct gctgtaaaag ctaaagaagc aatttcagct
     7621 ggtgtttgac ctgctaataa accaacaaag attcctaaat aaataccaat tgcaaatttt
     7681 gaaccccaga aaccaatttt agcatttaat ttagcagcat cgaagtcata tttatcaagc
     7741 caagggaaaa gcttgtcaaa aattttatcg aataccataa taattggatt catcatgtag
     7801 ttcatgtgag ttgttgtcat cggattttca tcaccagtac ccaataaatc attaaaagta
     7861 ggtttcataa ggtctgaatt gataattttt aaaataccga taaaaataac caaaatcgtt
     7921 gcaatcatga ggtttgctcc actataaata gcaaacaagc caacaattga taaatgccaa
     7981 atatcgaaaa tatcaacatc aagcgtattg gttttattca ataccaacat aatgatattt
     8041 acaatgacca ttaccatcaa aaaatatagg gtataaggtg agccccaagt gatagttgcc
     8101 aacggtgccc aaccaacgtc agtgatattt aattccaaac cagttctggc aacaaaatca
     8161 gccatagcac ccgaaaatgc cgttgacaag atattgataa ctgcgctaat acccgttaag
     8221 gcaattccta gtttaatacc accttctaat gctttagtga atttcacttt aaaaaagagg
     8281 gcaatgacag tcaagatgat tgtcattaac ggagcggcac caaggccaat aattggtttg
     8341 aaaatcgtat ttgcaaagtc gataattaca ttcatttcat ttcttccctt tctttcgaaa
     8401 gcgctttaat ttatttgttt aagctattaa taacgtcttc cattttctta taaacaggtt
     8461 ctgccattgc tgggatacga taaagaatag caccagcatc gacaactgga atcttaacat
     8521 caaagcctaa atcagtctta gaaatttgtg caaagatgtc ataatgcgat aacaattctt
     8581 cattgatatc tttaatcata acagcatcac acgatacttg ataaccacgt ttagtcaatt
     8641 ctgtttctag agcgttttta atttggtgac ttgagttaac cccagctcca cacgctgcta
     8701 aaattttaat catgagaaat tcctccttaa aaattatttt ccaaaaattg ataaattctt
     8761 tctggatttt cagaattaaa gaatttaatt aacgcttcct tatcttgtct attaataaaa
     8821 tccattattg ctgccaataa accagcttgc tctttttctt cattgtttaa aatcatgaac
     8881 aaaaatgaaa cctctagttt ttcatctggc aaaatcatat tatgaaaagt tatcgcatgt
     8941 tccagtttaa ctggaacaat acgagtaaca tttacaaact gactttctgt atgtggaata
     9001 gcaatattgg gaagactcga atcaattgga cttaacggta aggcagtcgg ataatgttcc
     9061 tctctgtcga ttaaattatc caagtattct tctgttacaa gacctttttc aaataattta
     9121 tgagcgattt ctttaaaaac acttttttga tcttgttgtt cagatatata taccgtatct
     9181 tctgcaaata attgagtcat tttatcacct tcttacactt tctcactcca tgagctggct
     9241 gttttgataa ggactgcgtt gagttcttcg atattcttgc gtccttgtgt tcttagccat
     9301 tcacgcgctg cggcctcgcc ttcttcgata tacacttgta cagagccagc ccaagtagca
     9361 cgtccgcaaa ggacaccatt gaatttagca cctgattcag ccgcaaatac caacgtttct
     9421 tggaaaagct ttgctgaaac acctgcgcta agatagatat atggtaaatg acttgctgct
     9481 tcttggtctt tgaaagcttt agcagcttct tctttagtgt aaaccacttc accgtccgca
     9541 aaaccttcaa catatttcat gttgacaggg acttcaacct tgagcacatc aataccaaag
     9601 cggtcgtcag aaaaaacttt catggcttcg ttaactttgt gaggttttac tttggcaaat
     9661 gctgcgcttg tattgtcagt gatgttttca tcataagtca aaatctcaag gaagaatggc
     9721 atatcttcag ctttacattc ggcaccaatg cgttcgatat aagcttgttt ttgaagattg
     9781 acgtatgaat ctccatccac atcataatag agtaggaatt taatagcatc tgccccttct
     9841 tcttttagtc gtttcacaga ccactctact aggcaatctg gtaagcgact agtagagctg
     9901 gtatcatagc cagttttttc ataagctagg agcaaaccgc actgttcgtc acgggcgcgt
     9961 gatgctggca aaccaaattc aggatctaac agaattgagg aagaaaactg tgttaattcc
    10021 tctgaaacta gtttctttag ttcttccatc tgcaccacag taggttcttc tacctgatgt
    10081 tttgccatca tttttttcag ggctccacgt tggtcgaaag ctaaagctga aataatacct
    10141 gcttcattgc ttactttttc catgtatttt cttttgtctg ctgttactac catattatac
    10201 ctcttcgact tcgatttgag aatacaacga ttctgcattt tctaaattga cgtaacccgt
    10261 ctgttcttcc tgggcattta acatgcctaa aacattagca ttttttaata attctgcatc
    10321 aggtaaagca tgtaccaaac ctgctgcaat acctgcaaca gttgaatctc ctgatcctac
    10381 tggattgacg actttgattt ttggaattct gacacgatag aacttgtcat tgtgcttggc
    10441 aaaagccccc tgtgccccta atgagacgac tatccaatca attccttgga aaagttgacc
    10501 ggacaaaact gatttcaact cttgaatatc atcagtaact tctttaccaa tcagttgtga
    10561 taactcctct gtattcggct taataactgt cggcttctgc tggctttcta ggaccttttt
    10621 cagcgcctca ccagaacaat ccaaaacaac agctacaccg tattttttac aaagttcaat
    10681 tattttaaca tagtagttac cagcaagtcc cttaggcaaa cttcctgaaa tgacaactac
    10741 atctatattg ttcagaataa tttcaaggtg ctcaataaaa ttcagggatt cgtgttcttg
    10801 aatagttggt ccttgttcta aaatctccgt ctgttgtcca tcgtgaagaa cagcaataca
    10861 gttacgagtt tcgttcccta tttctacaaa ggagtttttg atatcatttc gagtgagttg
    10921 tttctttata taacttccaa tctcaccgcc aataaagcct gttgctacaa catcttcacc
    10981 gatttgtttc aaaactcgtg taacattgag ccccttgcct ccagctgttt tttgaacttt
    11041 ttctactcga ttgaccgtat ccaaatgaaa ggtatccaat tgataggcaa tatcaacgga
    11101 cggatttaat gtgatcgtca aaatcatgtg agcctcctag tcgtggtatt ctccacgatc
    11161 ccacttttca agaaattccg taaagaactc tggattagcc tgctcttgat tgagcgtttc
    11221 aactgcagca atcttttcaa tcaaacattt gtttgcttca gttggtttat attctgcttt
    11281 aatgaaggct tcaataatgt cacacatgag cagctcgcct gtaatctttc ctccaaaacc
    11341 aatgacgttg gcatttaact cttcttttgc atagagtgca gaggtcatat cacgaaccag
    11401 ggctgatcga acaccaggaa ccttgtttac agcattgttg attccaacac ctgttccaca
    11461 gatacaaata cccaaatctg cttgcccgct tgcaacagct tcaccaactt ttttaccaaa
    11521 gattgggtaa tgagtccgaa cattgtcata agtcccaaag tccaacactt catatccttg
    11581 atttttcaaa taatctacca cagcgatttt ttcataggtt acaatgtggt cacaaccgat
    11641 tgcaattttc atctcttatt ctcctttgtt tacaacaatt agcacatttt atttagcata
    11701 tcaacacgaa tttggtgacg gccgccatca tactcacctt caacaaatcc tttaacgata
    11761 tttttagcaa ttccttctcc tacaatctca ctaccgattg taatgatacg agaattgttg
    11821 tgtcctcttg tcatataggc agagcgttcg tctgaaactt ctgcagcgat catgcctttg
    11881 actttagttg cggtcataaa tggaccaaca ccataagcat caatcacaat gccaagattt
    11941 ccttcctctt gattaacttc tgcaactact gccaaagttg tatcaacaaa atcacttcca
    12001 tcactgacat ctttaaagtc atatgatttt tctagcaaat aatctttaat aacgtctttt
    12061 agttttgaac ccgctggatc agcaccgata atgattgtca taacctatct cctaacaaaa
    12121 atagaaagtt gtagccatat ttggcagaat atctttgttt tgcaaaactt agaaaaagcg
    12181 ctttcgcttt ttatgtttaa attatacaac caaacaaata taaagtcaac atcttttgtt
    12241 ttatttttgt ttattttttt cgtacgcaaa caaaaaacca acatttctct gttggttttg
    12301 ataactttat gattttgtag aaataatttc gatgtgctta cttaataatt ggtaattttc
    12361 atcctttgca ttcatatcgg tcactagtgc agttacatca ttcaaatcac aaaaggaagt
    12421 aaaatcatct tttccaattt tcgaggcatc tagcagtaga tatttttcta aagaatgttt
    12481 aatggcaata ttttgcgtat aggcttccgc taaggttgag gtcatgacat cagttccctt
    12541 aagtccgtta cctgaaaaga aaatcttact gaatcgcatt ttttctaaac ttgtattggt
    12601 aatttctcct acaaaagatt ctgtcacttg gcgatattct cctcccaaga gaaagacttg
    12661 gaactcctcg gtcttctttt gagataggat ggtaaaaaca ggcatacagt ttgtgatgac
    12721 tgttaggcga ggatttttaa tagcctcagc cagaaaggcc actgttgttc ctggacctaa
    12781 gaacaccgtg tctccatctt ctatcagctg tgcagccctt tccgctatct cttgcttggc
    12841 ttccttgttc tgaatgtgtt tctcagaatg ggaatattcc cgatattgaa aagccttatt
    12901 acttctagca ccgccgtgga tttttgttaa gacaccttgc tcttccaatt caataaaatc
    12961 acgtcgaaca gtcatatcag taacatttag caattcaaca atctcagaaa ttctaactgt
    13021 accttttttg tttaccaatc gtgtaatttc ttctagtcgt tctcgtttat tcatgtttaa
    13081 tcctttgttg tttttttaca ttatatcaaa aataaacaaa aatatgttgg aattttgaac
    13141 attattttaa atgataatta caactttatt atgcgagcaa tcttcgcgag caggttttgc
    13201 gcggttcgca aaacatcggt tcgttagaac cgactgcaag gaaaaaagaa agcattgagt
    13261 taatccacac tcatcgtaca ctccataact attcaggttc atagcattgt tttgcaaaat
    13321 gggtttcaat taacatttcg ccagaacagt taatggcatt aggtaatgtt gaaaaagata
    13381 cggaaggttg gatagaactt gtcaatcaat acttacaata ttgtatagag attggtttaa
    13441 gtccatatac acaagcgacc tacaaagtag cattggccaa agttctcgga gtttcctcaa
    13501 ctaatttcat cgctacacaa ccacgtactc gtgcgaatcg tatgaacaat cgtgtattgc
    13561 ataaagacta tcgtttatcc aacaaaaata acgactactg gcataaagta gttaccgcga
    13621 caggcttacg caaaagtgaa ttgatccacg tcactggcga tgctctgcaa cgcggccgtg
    13681 atggccgttg gtatttaaat ttagccggcc ataagcatca cacaaaagaa cgcagagatc
    13741 gctggtctcc aatcatggcc actagtcaag aggaggaaga gtggttagtc gctattttcc
    13801 aacgagcagg agaaaagaaa gtctttcatg ttcccaaaga tttaatcctc gacgattttg
    13861 atggaaagaa agttccaaca gcattaaagt cacacaaata tcctgcagag tatgcagagc
    13921 gtgtttatcg cagtgtagca cgcgaaattt ccaagattag aaatcgtaaa gaagttatcc
    13981 atctgcgtaa agagttagtt gacatctcac tcgacagaaa agcttgtaaa attgtaacaa
    14041 aggctctggg acacaataga ccagaggaat ttcctcgttc ctacgcttat attttattga
    14101 aacgttaggt aacattgaac aattttcaaa aatctagcct caattcatta cgcatgtggc
    14161 ataatacttg tatctagaaa atgaaaggaa ttctatatgt tacctcaaaa taattctcct
    14221 cttctgttaa atagacaaca agcagcggaa ctacttggaa tagatcctaa atcatttgat
    14281 aagtacatca gaagtcaccc agacttccaa tgctttatgg tgggaaaaca agagcgctat
    14341 ttgaagtcaa aactggttaa atttattgaa agccactgcg attgacggat aatgattgat
    14401 aaaattagat ttttcatata tcctatatgt gactagtcta ttttatcaat cattttttat
    14461 agaaaggctg ataaaatggc aacaataaca aaacgtggta actcttaccg tgcaactgta
    14521 tcactttata agaaaggaaa atataaccga aaaactaaaa cctttagcaa cagaaaagat
    14581 gctgagcttt ggacattaga aatggaactt gaaaaaggtc gtggcaaaaa cattgccgaa
    14641 cgctccaccc tatttcctga tttttataga aattgggtcc atacggtcaa gaaaactgac
    14701 gttcgcgaag ccacctttat taactataaa cgaactctcg taatagttga tgaccttttt
    14761 gatggtattc aacttaaaca tcttgacgat ctcatcatgc aaaagaaaat cgaccaatac
    14821 gctgaaactc actctaaaaa aaacagtaaa agaacttgtt cttaaaatcc gtggttcatt
    14881 gaaatatgcg tatgctcgtg gtttgattag taatgacttt ggtcatcttt tgaaatcaaa
    14941 aggacaagaa caacctaaac gaaatatacc actatctatc acaaaattaa aaaaactcag
    15001 acaatactgc ctaagtcata ctgaggatga atttaatgtt cttgttgctc tcgcccttga
    15061 gacaggagca aggcgtggag aacttttagg aattaaaaaa gaagatattt ttgaatatgg
    15121 tatcaaaatt cttcgatcaa ttagtccaac taatgacgat acccagctta aaaccaaaca
    15181 ttctaagcgt gatatttcca tcaacgaaga tgtttatcaa gctgtgacaa aacttgcaca
    15241 aacaaaagaa ggctatatct ttgattggaa tggattcaag caagctagtc aacttcaaaa
    15301 gctgttaaaa cagttgggat taacaaaaac gacatttcat ggacttcgtg acacgcatgc
    15361 ctcatttctc ttttcaaaag atattagcct agactatatt tctcgtcgtc taggtcataa
    15421 ctctatctta acaacacaac agtattatct agaattgata ccagaaaaaa agcaccagca
    15481 agatgccgat gctttaagtc tcttaaacga cttatcatta taattaacac caactggcac
    15541 caaaaaagcg ccataatcgt tggtataata aggttttgat taacgttttt gaactataat
    15601 tcaaaaacat tcactccaat ttttattagc tgaaatataa tcctataaac gcatcattag
    15661 acttctttct ttaagattct aagctgatgc gctttttatt tatctgttac tccaatcagc
    15721 atgaaatgtt ttcctacaac tgtatcagtt aactgactag cttaaaaatt cctgtccaat
    15781 ttagaaatgc ctttcgacgc atttgttcaa tttcacagcg aaataatcct gctaaggttg
    15841 ctctatttaa aacaatatca ttcttacgag aaaggctagt tactttattt acaagcgtct
    15901 cgacttcttc accatctcta attcccatat cccataacta ttgaagtact gattttttat
    15961 acattttctc atattctgcc ataccttgta gagtccagta acgttcatca ttctcaactt
    16021 ctaaccattg ccacacaatt tctgtatcag ctacattagg tttttcatgc tgacaggata
    16081 aacagaggac catctgatta gccaggctcg tatccttgct ccatttgctt gatacaatct
    16141 gacacacagc tacaccatga gaacttttac atctccagca ttttccttca agcaattcat
    16201 ccgcgctcaa aattcccctt tctatcaaag tatcattcca atattctgca actttcgata
    16261 cttcagcttt cttattcaac aaattaaaca ttttcattct cctactataa ttacttttct
    16321 aatatctgtg tccgaaccat actagcctgc aacattgcta actcaactac acaagaataa
    16381 tgactcaaat caagattttc caacaaacga taagatacaa ataacttcgg ttgcaatcgt
    16441 gtgagaggtt ttgaccatat taccgcttct gactggaatt gtttgactgc ctgaaaataa
    16501 atctgattga ctgtaaggta ggctatacca tttagtaaat atgcataatc ttgtattaaa
    16561 tctctacaaa atgaagctac ccaaatagct gcggtcttct cagaatcagt atcgtaatcg
    16621 gctaataagt ccagcgtaat cagacttcct tttccaaaat ccttcgattt ctgatctcgc
    16681 aatttttcag tatttatcgg agtcgtcaaa ccaaaatgat tagcataggt ttcccactct
    16741 ttataaatat cattccaaga aatctctgta atccttttca tttgcgactc ctatttttct
    16801 tttttgtgag attatcataa caccgttttg attttttgtc aaacatgtta aacaaaattt
    16861 gcaaaaaata ttttttaatg gtataattag ttaaacagat aaggagacaa ccatgatttt
    16921 aggtgatatt ttaaaagaat accgaagcaa gaataaatta tcaatggata aattcgctga
    16981 attatctggt ttaacaaaag gttatatttc tatgttggaa aagaaccagc atcccaaaac
    17041 caaaaaagct ctcctgccaa cgatggatac gctagaaaaa gtcgctaagg gcatggctat
    17101 atcggttggt gagctaatcg agcaactaga ttctaatcag gatatagctt taacgattac
    17161 tccttcccaa ttcaaactct ttcaacagcc tattcatcca gaggttcaaa ccttggacaa
    17221 tgaattaaaa ggtgatttcc atgcacaatg gttaactttt ggtcaagaac agttagcagc
    17281 tatgcaaact gcctcagtta ctccttctgc ttctattctt cacttggatg actatcggga
    17341 gcaaagcgaa ttacctgttc ctggtaaggt ttctgccggt acaggttact ggcaggatac
    17401 agatatgaac aatcttgtat cattctataa cgatgagatt ccagataacc aatcttatga
    17461 tacaatcgct caggttgtgg gagattctat ggctccgcat atcgaggatg gagactatct
    17521 cttcattcgc ctaactcctc atattcctct aaatacgatt ggaatttttt cagtcaatgg
    17581 ggaaaatttt gttaaaaagt tcaaaggtga ctatttgcaa tcactcaatt ctgactatga
    17641 tgatatctat tttagcccag aagacgatat tcgacctatt ggtcaagttg tagatattca
    17701 tagtccagat tgatttctaa gcctagttaa tcactagtta tattggctac attcatccat
    17761 ctagttttgg aaagtctgtt cgtatagctg gcgttttttc ctactgaaaa caaactgaga
    17821 agcacagtaa aaggaggcta tgacggcctc ctttttatat gtcaaattgg aaaagtccat
    17881 taccagatag ttatttgaca acaagttaat gattaaataa ttattctgct acccttatgt
    17941 ctttgattgt cctcttacta tttgtctaaa gtgtggagat tgcttattat gatgaaaatc
    18001 gctagaggtt tttgtgattt tctaaggtta tgttcaaaag ttacaacatc ttgaacacat
    18061 agtagttacg acacgaataa tcttagccta cttctgattg aaccgggtag caaggctaaa
    18121 taatgagtta atgctatgca tttgacaatg gatatcacaa atagatataa aacagggata
    18181 acggactttt cccctactag catccctctc tttttattag agaaagatgg ctaatagtat
    18241 atacacttct acgaacgcta aagaactcct ttcagcaatt cagatggcgt tcaacatctt
    18301 aactaactat ttcctttttt caaatagatt ataccactat tgtaaaacgc tttcaaataa
    18361 ttatgtgctc caaagtcaac ttttttgctt ttctttttcc ttgataaagt tttcccacat
    18421 cttttgttgc ggcttagcaa atgggtaggt aggaaaatct tctacagaaa cccatttcaa
    18481 ttcttttgat tctggaaggt taagtgtttc tgccacacct tctaccattt ctatctgcca
    18541 tttgcggtga ctaaaaatgt gctggacgat gccaaattct tgctcttgcc aatcaacagt
    18601 caaaccataa agttccgtaa agctctgccg aatatctggc tgaacaactt cttctgcaac
    18661 ttcaaagaga gagacttgtt tttcaacgat tgctcctttt tccaaaagcg gaaaagacca
    18721 gaaacctgat agaagacctg cttcattatt tttttccaat aaaaattgat tgtctttatt
    18781 tctgatgaga aatccctggt aggccacggg aatcggcttt ttctttggtg ctttgattgg
    18841 atacttatcc attgtaccgt ttaagtaggc tgcgctaaag gctttgactg ggctgtcctg
    18901 gggacgtgga ttaactggcg attcaatatc cgcccccaga tccatcaggg cttggttgaa
    18961 attgcccggt cgctctggat caatcaatag ctccatcatc gcttggaaaa tcttacgatt
    19021 ggcaggaaga ccaatgtcat aatcaacctc aaacaagcgg ctgagaaccc gcatcacatt
    19081 gccatctacc gctggttctg gcaaattaaa agcaatactg gaaattgctc cagcagtata
    19141 gggaccaatg ccctttaggc ttgaaatggc tgcatgggtg gtcggaaatt gcccatcaaa
    19201 atcttctacc atttgctgag ccgccttttg catattccgc acgcgcgaat aatagcccaa
    19261 tccctcccag agtttgagaa tgacctcctc tggtgcttgt gctaaatcac taatagtagg
    19321 caagtgatgg agaaaacgtt cataataagg aatgaccgtg tctactcttg tctgttgaag
    19381 catgatttca gaaacccata tagcatacgg atccttggtc cgtctccaag gcaagtcacg
    19441 tttattggca tcataccaat ctaagagtgc cttgcgaaaa gcttgaatct tttcctccgg
    19501 ccacatttcg attccatatt ctttcaaatt taacatacat ctagtataac acaaagaaag
    19561 agagccagct tttgccaact cccttatatc gtttataatc aaaatcaaac tagctccaaa
    19621 ggtttgggga aacttgggag gttggaaata gagcgaacga agttcgtttc tacaaaggtt
    19681 tggggaaact tgggagattg gaaatagagc gaacgaattt gtttctctaa atctagccaa
    19741 gactgtctgt ttttgatgtt cgttgagtat tattctactt ttcctgttac aaacatggta
    19801 aaactcgctg aagcaagtag ctgtttatct tgattttcta tcttaacttc gacgaccttt
    19861 gttctacttc catcatgtgt acaagagcct attactgata aggtatcacc aagtttagct
    19921 gccttcatat aatggatatt ggactgtagg gtgacagaat aagaacccct tgctaccgtt
    19981 gtcagacctg caactgaatc tgccaatgtg aaaagaaagc ctccatgtgc cataccgtat
    20041 ggattgagag atttttccgt cacctttgta gtcagtagca cctctttctc attgagcatt
    20101 gtagcttgat agtcatctaa aaccgtaatc ggttgtaagt ttaatttatc taacactttc
    20161 atcctcctct gtctgctgac ttgtttgcat ttgataataa acgccttgtt cagccatcaa
    20221 cgcttcatga ttcccttgtt caacaatttt tccatccacc atgaccaaaa tcaagtcagc
    20281 tgattgaatg gttgataggc gatgagcaat aataaaactg gtccgtcctt ccatcaattt
    20341 ggcaaatgct tcctgtacta aaatttctgt ccgtgtatca atagacgaag tcgcctcatc
    20401 taaaatcagg attttaggta tcttgacaaa gacacgcgca atagacaaaa gctgacgttg
    20461 tccctgggat aagccttcac ctccatctgc caaaactgta tcgtagccct gcggaagctg
    20521 acgaataaag aagtctgcat tagctgcctt agccgcctca atgaccaatt cccgcgtggc
    20581 atttggatag ccataggcaa tattatcatg aatggtacca gatttgatcc atgtctcctg
    20641 taaaaccatc ccaatctgtc tgcggagatc ctcacgactg tactggctaa tcggaacccc
    20701 atctaagaca atctgaccag ccgttacatc gtagaatcgc ataagcaaat taatcatggt
    20761 tgatttacca gcaccagtag gtccaacaat ggctaccttt tgtcctgctt tgacatcaat
    20821 gtccagatgt tctatcagag aacggtcagc actatatgag aaagacacat tgtcaaagga
    20881 aatagctccc ttcaactcct ctgaatcgat tttccgctca gcctgatcgt caatgctatc
    20941 taaagccaag atagcaaaca gcctgtctgc acaagctaag gcgctctgca attccgatag
    21001 gaccgaggaa atatcattaa aaggcttgct gtactgacta gcataattga ggaaggttgt
    21061 cagtgaaccc actgtaaact gccctgccat aattctcaac gctccaagtc ctgcaagtaa
    21121 ggcataaata atagcattga taaaccgcgt gcttggattg accgttgaag aggcaaagat
    21181 agcctgttgg gaataggtcg catactggtc attcacaact tgaaaacctt gcgtaaattg
    21241 ctcttgagca ttgaaagatt gaaccagtgt caattgactg atggactctt ccaaaagctg
    21301 agattgcctc ccacgtgcct gagtctgttg cctataataa ccataggatt tcttagcgat
    21361 atagcgtgcc acaagcagag aaactggagt cagggcaaca acaaccaaca tcatcatcaa
    21421 gtcaagccta gccatagtga aaatggttag gaaaatggtc aagataccaa tgaaaaattg
    21481 attgaaaatc atcaagagac catttgttaa ttgctcacta tcacttgaga aacgagctac
    21541 taaatcacca actgaatgac gatccagata agataggggc atctggtgca gcttaatata
    21601 aacctgttca cgcaagtcgg ccaccattcc ataaaccaag cgattggtta ccaaaggtaa
    21661 gtaccattgc accagtgtat taagcaagat aaccatcacc attttgccta agataccgag
    21721 caagacctga ccatttgcta tcaatactgc atcaactgcc tgaccaatca agataggcag
    21781 ataaactgtc agaaggactt gcaccaaagt tcccaccaac atcaaggaca ttcgaagggg
    21841 ctggtgtaga accaatctga ccagttcctt aaaactagat tgtctcatag ctatcctcct
    21901 ctccttgttg ttgcgattgg tgaatttctt gataaatagc agagcttctc agcaagtcct
    21961 catggcgacc aagtccaact tgacgccctt gttccaagac taaaatctga tcagctgtcc
    22021 gtaaactatt ggttctttgt gacaccatga ttaagttctg acctggtaat tcctggcgaa
    22081 tagccactaa taaccgactc tccgtcaaat aatccagagc agaggtcgca tcatccaaga
    22141 ttaaaatcgg tgctttctgt aaaatagcgc gtgcaatggt caaacgttga cgctgtccac
    22201 cagaaaaatt cttcccaaag gcttcgacag ggctatccaa accaccttta tcctctatga
    22261 aagatttagc ctgtgcaatt tctaatgcag accataggtc actatcagat atttcttcca
    22321 atcctaaaga taaattagaa cgaatagttc cagcaaaaag ctgagcctgt tgcggaacga
    22381 tggcaattcg ctgccgccat tcttttaaat tcttaggact ttttccatca atgaagagag
    22441 agagctgatt tgttggcact gaatagagtg cctgtaataa atctactagg gtggacttac
    22501 ctgacccagt tcccccgata attcccataa actgtccctt acgtaagtca aaagcaatgt
    22561 cagacaggga ctcctcagca gattttggat aagaaaagga aagatgacgc acagaaaaga
    22621 tgatttcctt atcctcacta accacttttg gcaaactaga ctctacatcc tcagatgttt
    22681 ggtcaaaaac ctcttgaata cgttgcgctg aaatataggt ctggttgaga gtagacacga
    22741 ccataatcat cttgaccaat tcgactaaaa tttgaagaag gtagttgata agagcgacca
    22801 acatcccctg ttccaacaaa ttgtgtgaaa tggctatatt tccttgccag atgagaatga
    22861 gtaaggtgcc gttgaccact aaaaatgtca atggcgataa aagactagac caaaaaccag
    22921 cttgcaactg ttgcttctta taaatctgat ttatgccttg aaaagcctta atttcccgct
    22981 ctctttgtcc aaatgcacga atcactcgcc aaccagtcac tgtctcacgt acctgcccca
    23041 ccaaaccatc taattctttt cgcatcaatt gataaatacg gctggtcata acagaaatga
    23101 ctgtaactat aaaaatcaat aggataatca ttcctagaaa ataggcagat agacttgggc
    23161 taatatagaa tgccatgatg agcgaaccaa atacgacaat tggagcacgt aaaaacaaac
    23221 gaagaaaagt attaataccc gtttgaattt gtaaagtgtc gctagtcaat cgtgtcagca
    23281 ggctggatga ggaaagaata tctcggctag acttgggaag ggaaagaacc ttctgataca
    23341 agtcattcgt cagctcttta gtcacaccaa ccgctgcttt agctgaataa tattgcgcta
    23401 ttagtgaaac aataattcca atacatgcta taccaaccag taaaaaaagc atagcaacca
    23461 ggtttccctg gctgccattt ggaataattg tatcaacgat ataggcgata attaatggta
    23521 ccaaaagctc aaaacaagct tccaatagtt tgaaaactgg acctaatatg gattccttaa
    23581 tatagtcctt aaaatatcgt gataaaaatt tcatattaac ggtgctccaa agcctgaacc
    23641 gcagtaatca aggtcaagtt gtaaacatca tcagggttac aaccacgaga caagtcgttt
    23701 acaggctgat tcaaaccttg taaaacagga cctaccgctg caaatccacc catacgttcg
    23761 gcaattttat aaccaatatt accagctgag atacctggga agataaatac agtcgcttga
    23821 ccagcaacac tcgaacctgg ggcttttaat ttacctgtgg taggaacaaa ggcagcatca
    23881 aattgcaatt caccgtccaa atccaactca ggacgcaatt tttgagcaat ttttgtagct
    23941 tcaacaacct tatctacttt aggaccagca cctgagcctt ttgtagaata gctaagcatt
    24001 gcaacttttg gatcaatacc aaacatttta gcagtacgtg ctgagttgat agcgatttct
    24061 gccaaggttt gagcatctgg atcaatgttg atagcacagt caccaaaaat ataacgctct
    24121 tcacctttta ccatcaagaa ggcacctgat gttttagaaa cacctggctc agtcttaata
    24181 atttgaagcg caggacgaac tgttgaggca gttgagtgaa tagcaccaga aaccattcct
    24241 tctacaacgc ccatgtaaac caacatgaca ccaaagtagt taacatcttt caacaactca
    24301 tgtgcctgct cttctgatac cttaccttta cggcgctcta ccaaagcagc taccatctca
    24361 tcatatctat cataatgagc tgggtcaata atttcaaaac cttttgtaat cccttcaatg
    24421 tcaaggtaaa tcttaatacg atctgggttt ccaagcaaga cgggtactac atccgactca
    24481 tttaccaaac gttttgtcgc ctgcaagata cgtggttctt caccttctgg caatacgata
    24541 cgtacttttt taccctctag ctttgtagct aattgctcaa aaattttcat gaacaaactc
    24601 cttaaattta tttacttatt aactgattga tgacagcctg aaaatcctca ggaagcggtg
    24661 cttccaaatc aatcctttcg gctgaaaaag gattgtcgaa ggccagatta tgacaatgta
    24721 aggcttgacg ttctatccca cattccaaac ttccaccata catatcatct cctaacaatg
    24781 gaaatccaat gtgagaaaaa tgaacacgaa tctgatgagt ccgcccagta tgtagttgaa
    24841 tatctactaa atgaatatct ccccacgatt gcacaacacg ataggaagta tgagcatatt
    24901 ttccattctt tgtcacacaa cgggtaatga tgctgtcttc tggacgaccg ataggggcaa
    24961 taatatcccc tgtctcttcc agctgcccat tccccttcac caaagcataa taacgtttat
    25021 gaataagctt ggcctgtaac tgcttatcga gtcgtgcatg ggcatagccg tgcttggcaa
    25081 acagcataac gcctgatgta tccctatcca gacgagtcac aatatgaacc tgcttattct
    25141 cataattctg ctcaataaga taacctttta caaaatttgc gattgtggat gaatgcaagg
    25201 cagaaggaat agaggcgtag ccaaccggct tatttatcgc cagaaaatga tcatcttcat
    25261 agacaatatc caacggaaag aaaatcggtt ttaaactacc agtcaaatcc tcttcagcag
    25321 gtatgtcaat tgtaacacta tctccaatat caagcaagta agttgcattt tgctctatat
    25381 cattgaccca gatattgccg ccggtatact taatcttcgc taataggcct ttagagacac
    25441 cgtgtttttt taggaaagtt tttattttcg tatgctggtc agcaatgaat tcaaaccgca
    25501 ttactccacc tctccaataa aggcgtcctt aacccgattc caaaaactag tatgactcgg
    25561 tgttgcaaca aaatgaatct tactattgtc tatctgatac tctatacttt caatactatc
    25621 atagacaaaa gtcttattgt ctaccgcaat tgagtaacgg tcactatgct ttggttcaat
    25681 cacaatcttg tcttttttag ggacaacaac ggatgaaccc aaggtgcggt aaacacgatt
    25741 gttcaaactt gccacttctg caatctgcaa agcctcgatt gttggatgta aaacagcacc
    25801 acctaatgac ttgttatagg ctgttgagcc tgtcggcgtt gaaaccgaaa ttccatcccc
    25861 tctaaagcgt tcgaagggca cattgttaat aataatatct gcgaccatgg tcttagaaag
    25921 acgttttact gtcgcctcat ttaaggcacg cgcctcgaca atgcggccat ctgtcatctt
    25981 gaccttaaca tttaaaatcg gatacgatac tctagcacct gtatcaagct taagattttc
    26041 aatcagcttg gccacttcaa aatcgcgata atctgtataa aaccccaaat gacccgtgtg
    26101 aattcctaca aaacgtacac gatcaatcaa cttttcatat ttgtgaaaag cagataaaag
    26161 catcccatct ccaccgatag aaatgacaat atctggattt ttaggagtca aaataaaatt
    26221 tgcttctttt aactttgtcc ataattcttt ggacaccgct tcactcttag gatttcgact
    26281 agcgagcaga gcgatttttt ttctacctgt attcttcatc tgtatcatca ctgttgccca
    26341 caccgtcatt cagtttgcga taggcaggat caaataaggc ctgcgcttct tgaatgtcgt
    26401 tgcggatttt tcgcatctcc tcatctaatt cataggcaat ttttgctgta acttccaagc
    26461 gtttcttgat ttcttctgga aaattcccct tatatttata attcagagaa tgttcaattg
    26521 tagcccagaa attcatggaa agggttcgaa tctggatttc tgccagaacc gtctcgttgc
    26581 catttatagt atccacagga tactcaatca ccacatgata tgaacggtag ccagaggctt
    26641 tcatattatt gatataatcc cgttcatgca caatttgcat atccttgcgt ttccgcaaaa
    26701 tagctaatac ttcttcaatg tcgtccacaa attgaaccat aagccgaaga ccggcaatat
    26761 cttgcatatc ttgagcaaga ttttccaatt tgatatgtcg cagggccatt ttttctttaa
    26821 tggattcaat cggttttacc cgtcctgtga caaactcaat tggagaatgc cggttagcct
    26881 tgcgatactg cttgcgaatc ccccgcagtt tgattttcaa ttcaccaact gtctgaatat
    26941 aggggtctaa aaattcttcc cagttaaagt ccatacggcc cctttcttgt caaaactttt
    27001 aaattgtatt agaataacac aaagactaat ctattataac acattgaaag ggtttactgc
    27061 atgaaaaacc accttgaaat tgagtataaa accttactga ccaaatccga atacaaacgc
    27121 ctgcttactg acttttcaga tgtaagccca attcttcaaa caaaccacta cattgatacc
    27181 ccagacctag atatgaagaa tcaccgcttt tcactccgta ttcgtacatt tgaggactca
    27241 gcagaactaa ccttaaaaat tccacaagaa gtcggcaatc aagaatataa ccaagttcta
    27301 gacagccaaa ctgctcatga tttactggaa aattttcaac taccagcagg acaaatttct
    27361 gacattattt ctgcaaccga aattccactt gataaacttg ctgtctgggg aagcctcacg
    27421 acaaaacgtt atgagaaaca gacatctata ggccttatgg ccctcgatga aaatgattat
    27481 gtgggacaaa aagatttcga attagaagtt gaagtgaccg atgcagatgc ggggaaaatt
    27541 ctttttgatg aattccttaa gaagaaatca attcaattca aatatgctag tagtaaggtc
    27601 gcacgaacag ctgcccacat gaaataacta taaaaaatca gccaaataag gttgattttt
    27661 tagtttcact actgaaaatt tttcattttt ttgatagaat agaactatct attgtaaaaa
    27721 atacttttat gtcatcataa aagctcagtg tgtaaaagga gttacttcaa aatggtacaa
    27781 ccactcggtg aacataacat taaattattc tcactcaata gcaaccgtga tattgctgaa
    27841 aaaattgctg attctgctgg aattcctctt ggaaagctgt cttctcgtca gttttctgat
    27901 ggtgagattc aaattaacat tgaagaatct gtacgtggcg tagacgtcta cattatccaa
    27961 tcaacaagct atccggtcaa taatcacttg tgggaattat tgattatgat cgatgcttgt
    28021 aaacgtgcta gtgccaacac cattacagcg gtcatccctt atttcggcta tgctcgtcaa
    28081 gaccgtaccg cttctccacg cgaaccaatt acagctaaat tagttgcaaa tatgctggtt
    28141 aaagctggtg tcgaccgtgt attgactctt gatttgcacg cttcacaaat tcaaggtttc
    28201 tttgatattc cagtagacaa tctccttaca gaaccacttt ttgcagccta ctacatggaa
    28261 aaaggactct gcggagaaga tgtcgtcatt gtgagtccta aaaattctgg tatcaaacac
    28321 gcgcgtaata tcgctgaatt tctaaatgca ccgattgcga ttatcgacta cgttcaagat
    28381 gattctaagc gttctgaagg ctatattatc ggtgatgttg ctggtaaaaa agctattttg
    28441 gtggacgata ttctcaatac tggtcgcact ttctcacaag cttcaaaaat tgtccaagac
    28501 ggtggtgcta ctgaaattta tgcagttgcc agccatggtc tctttgcggg aactgcagcc
    28561 caactcttag atgaagcacc aatcaaagaa atattagtta ccgattctgt tgcaagcaaa
    28621 gaacagcatc ctaaaaatat cgccttccta acagctagtg acttgattgc agatgctatc
    28681 caccgcatcc aagaacatca gccactcagc ccacttttca aatttactag tcctgaaaaa
    28741 gaaaactagg agtaactctt tgatttattt agataatgca gcaaccactg ctctatctcc
    28801 tactgcactc caacggatga tggaagtggc tcaagaaaat tatggaaacc cctccagtat
    28861 tcatcaaagc ggtcggaaag ccaatcaatg tcttagacaa agtcgtcaag agattgctca
    28921 gattctctac gcctctcccg aacaaattat tttcacatct ggaggctccg aagctgatac
    28981 tttagctatt caaggctatg cacttgcaca tcaatccaaa ggaaaacacc tgattactac
    29041 ggctatcgaa caccatgctg tcctccatac cgtgcagtat ctagaggaac ggtttgggtt
    29101 cgaggtaacc tatatccaac cagtcgacca agtaatcacc gctcaacaaa ttcaggatgc
    29161 ccttcggcca gatactattt tagtcagcgt catgtttgct aacaatgaga ccggtcagct
    29221 acttcccatc aaggaaattg gagagctcct agccgatcat caggccgtct ttcatgtaga
    29281 tgctgtccaa acaattggaa aaattgcaat ttctccatct gactacggaa ttgacttgct
    29341 cgcggcctct gcccataaat tccatggtcc aaagggaacg ggattcctgt atagtaaagt
    29401 tcataaattt gattccttga tccacggcgg aaagcaggaa cagcaacatc gagcaggtac
    29461 tgagaatctc cctgctattg ctgctatggc gactgcccta acagaacaat atgagtcctt
    29521 agatacgcat catcaacatg tcagtgaact gcgacaacat attatcgagg gattgtctgg
    29581 cttagactat tatctcaatc aggctggacc tcatctccct catgtcctta atatcggttt
    29641 tcctggtaaa cttaatgaac aactactcat gcaactagat ttagcaggta tcgctgtttc
    29701 tagtggttct gcatgtacgg caggcgttgt ccaaaatagc catgttttag aagctatgta
    29761 tggaaaagac tcccatcgtc tgaaagaatc cattcgtatc agcctatctg aaatcaatac
    29821 aaaagaagag atagatactc ttatcatcga actaaagaaa atacttggag gttaaaatgg
    29881 cattccaaca atcagttacc ttaaaagatt gtaattttac ttatcacatt agccctgcta
    29941 tcaaaaaata tacactccgt gataacacct ttgaacaaac caaagcaggt cactatcagc
    30001 ttacacgtct tctagaagaa attccaaatt caaaccaagg attcctcttg aaaattatta
    30061 tcaatcaaga cctaactggt tttaaaatta acattaccga caagtctggt cttcatctgg
    30121 taaacatttt taagccagaa agtaacaagg ttatccaaga aaaattctat ttcctcatgg
    30181 atagcttgat tgaccgtgac atctttacaa aagaggctta gtatgattga aattcactat
    30241 cttgacgctt acaagcaaga acgtatccaa acatttgaga ataaagatgc agccattctg
    30301 gctttttccg gttgcttaac cctcccagac tattatccag ttaccagcat tacacaagac
    30361 ggacaagctc tggattacaa agggacaatc ggagatctct atcgctattt acaaacacta
    30421 gattagacag tttcctgttt aatctttttt taaaatcaat ctgttgattt tttcacaaac
    30481 ttggactaga atagaataaa gaactgttta atatataaat aattgataaa gcgtattcta
    30541 tcgaaataaa ggagtctaaa gtgaaaaacg ataaaaaatc tgatattcca cgcgctactg
    30601 caaaacgtct atccctctac tatcgcatgt ttaaacgttt ctatgcagga aatattgaaa
    30661 aagctagttc aaaagaaatt gccgaagcta tcggaatcga ttcagctacc gtccgccgag
    30721 atttttccta ttttggagag cttggtcgtc gtggctttgg ctacaatgtc aaagagttga
    30781 tggatttctt tgcagatatt ctaaacgata cctccatcac caatgttatg cttgtaggtg
    30841 tcgggaatat ggggcgggcc ttactacact atcgcttcca cgaacgaaat aaaatgaaaa
    30901 tagtcatggc ttttgaagca gatgataatc cagctgttgg aacgacagat gaaaatatcc
    30961 caatccatgc aatctcagag atcaaggaac gtatcagcga agcaaactct cagacagcca
    31021 ttctaacggt acccagcgtc aaggcacaag aagtgaccga tattcttgtt gaagctggtg
    31081 tcaagggaat tctgagcttt tcaccagtga acttgtctgt cccaaaagat gtcgttgtcc
    31141 agtatgttga tttgactagt gaattacaga cactactcta ctttatgcga aaaggataag
    31201 caatgaaaaa accagttatt ggtatttctg gaaatgaata taaaacaggg gatcatacag
    31261 aacccctgct ttcttatacc cagacttgcc tcgtacaagc tattgaagat gctggcggac
    31321 tcccactcat ccttcctgtt atagagccag atttggcaaa atattatatt catcttattg
    31381 acaaactcat cctaacagga ggacaaaatg tccagccaag ttactatcat gaagaaagga
    31441 ctattgacag cgataattat ctaccaaagc gtgacgagtt tgaattggcc ctcataaggg
    31501 ctgcccaaga aaatcagaaa cctatcttcg gtatctgccg aggtctacag ctctacaatg
    31561 tagctcaagg tggtagcctt catcaatcaa tttctgaaca ttggcaagac atagatggac
    31621 aagaagtcag tcaaacgatt caactgactc aaaatagtct actttacgac atatataagt
    31681 ccgatccgag tgtgaactca tttcaccgtc aggctatcaa agacttagcc cctgatttag
    31741 aaatcattgc actatcagat aatcaacaga ttattgaagc agttcattca gcctacccaa
    31801 ctaaattttt aggggttcaa tggcatcctg aactactcta cggtaaacgt gagattgaaa
    31861 aagaactctt ccactatatt gtcaataaac tttaaaataa ctcactttct tctctaaaag
    31921 aatagtaatc cttgtttcca acaattaaat gatctaataa taccagtcct agcttttcac
    31981 aggactcttt taaattctcc gtaaatagca aatcattcct actaggttgt actgaacccg
    32041 acgggtggtt atgaacgatg ataatcgatg ttgccataca ttttactgcg taatgcaaaa
    32101 tttcacgtgg ttctgcaatg ctacgattaa cactcccaat aaaaatcgtt ttttgactga
    32161 taatctggtt ctgggtattg agataaagtg caactagatg ctcctgcttc ttatgcccga
    32221 tttcatgaat catcttacgt cccaacttct cacttcccaa aattctctca tttaagagca
    32281 actctgattg attgatacgt tttccaagtt caatcatagc cttaatttca atcgccttga
    32341 cacgcccaat tccagttaaa ctttgcaatt cttcaattga tagttctctt agagccgcta
    32401 aactctccaa acgattcaat aatttattgg agagaataga aacaggttct ttttttgtcc
    32461 cagttctaat aaaaattgct agtagttcct gattactgag tcgctcagct ccaacttcta
    32521 ctaaacgttc tctaggtaga atggcctctt ctttaaattc aatttgatac ataatttcct
    32581 cctcacctat ctattcgtaa aatcttgcaa aaaaagaagc catataaaac caaagattat
    32641 ctcctcagtc ttataccact tctttatcct taatatatag atgttttatt gtccataatc
    32701 atactgatta taactcttat tgaaggaatc taagatgtcg tttgccgttt cattatatgg
    32761 agtggttgat tcaagaactc cctttacaat gatacgttga gttgcattat agtccgtaca
    32821 tgtcactaac gtaacttctg tacgtccttc tacatcatca atgacataga cactttcagg
    32881 cgaaacaatt tccacactat caataacgta ggtgtaaaca ttggtcttgt cagtaatata
    32941 gattttcatg ccgtttttag cacgatcaag cggtgaaaag agaacatctg ccgcaccagt
    33001 tacaccaaaa atattgtggc tggccaatgc ataatttcct ttccccatct cttggttttc
    33061 cttcatagta ccagctccat acatgagcga agtattgaag acccctttaa aaataggaag
    33121 gttgatacca agctcgggaa ccgcaatccc accaattaca ggtaagcgtt gtgcatccca
    33181 ctgagctgct aaaatagcct ctgtagaaat agactgaacc tgatcgaaat caaatgttgt
    33241 ttcagcctgt ttattctttt cgatatcctc tgtcgtaaca ttgctaatct ggtatttatt
    33301 cgtattccag ccgataataa aattacggat agaggtatta aatatcaatg ccaaggaaat
    33361 cagtatcaat acaactgcta aaaaattgcg ccaaaaagag cctttacgct tcttcttttt
    33421 ttcacgtttt gacatgatta tttattcctt ttcttctttg atctcggcgg catctaccaa
    33481 ggtaaaggtc atgattttcg cttcatcatt caagcgcatc acttttacac ccatggttga
    33541 acgaccagtt tgagagatat tggcaacact tgtacgaata atcacgccag tatcggtaat
    33601 aaccataata tcttcatcac cagataccgt tgtcaaacct gccaaagaac cattcttatc
    33661 agcaacttta agagtcttga tacccttacc accacgacct ttggttggat actcactggc
    33721 tggtgtacgt ttaccaaaac ctttttccgt caatacgagt acttcttggt catcagaaat
    33781 catagtagcg ccaactaact ggtctccctc gcgtaaattg ataccacgaa cacctgttgc
    33841 tgtccggccc atattacgaa catctgtttc agtgaaacgt atagaataac cgaacttagt
    33901 ccccataata atgtctgctt gaccatttgt taagaaaaca ttgatgagtt catcgtcttt
    33961 tttcaaattc aaagctttca gaccgctctg gcgaatattg gcaaattctg acacactagt
    34021 ccgttttaca acaccttggc gggttgcaaa gaagagatag ctatccgcat ccaggtcctt
    34081 agttacattg ataatggttt gaattgcctc gttttcttcc aatttaagta aattgactac
    34141 tggaagtccc tttgcagtac gaccatattc tgggatttca tagcctttga gacggtaaac
    34201 acgtccctta ttagtgaaga aaagcaagcg atcatgagtg cttgtagaga ctaattctcg
    34261 tacaaaatca tcatccttga cgccagttcc ttgaacccca cgtcctccac gcttctgagc
    34321 ttgaaactca tcctgtgcca aacgcttgat gtagccttgg tttgacaagg tgattaaaac
    34381 atccgtttct tcaatcaagt cctcatcttc aagggaaaga acttcaccaa ccatcaattc
    34441 tgtacggcgt gggtcagcat atttacgttt gacttcttct aattcgtctt taataatctg
    34501 aacaacacgg tcaggcttag ctagaatgtc agccaaatct gcaatcaagg ccactaactc
    34561 atcatattct gattgaatct tgtcgcgttc caaacctgtc aaacgacgaa gacgcatatc
    34621 aagaatagcc tgactctgac gttcagaaag gtcaaatttc cccatcaatt cagcttgagc
    34681 aatagcatcc gtttctgaat tgcggataat gcgaatgact tcatcaatat ggtcaagagc
    34741 aatcagcaag ccttctaaga tatgagcacg cgcctcagcc ttttctttat caaatcgtgt
    34801 acgacgagtg actacttctt tttggtgttc aatataagac tctagaatct gacgtacaga
    34861 aaggattttc ggtacactat tttggatggc caacatgttg aaactaaagt tggtttgaag
    34921 ctgtgtttgc ttgaataggt tattcaaaat aacgttagca gaagcatcac gacgaacttc
    34981 gataacaaat cgtacaccct cacggttgga ttcgtctcga acagcagtga ttccttcaat
    35041 acgtttttct tggactaatt taacaatatg ttcttgaacc ttggacttat tgaccatgta
    35101 tggaaactct gtaacaacaa tgcgttcacg accatttcca tattcttcaa tttctgtgcg
    35161 ggaacgaaga actattgaac ctttaccagt ttcataagca cggtgaattc cagatttccc
    35221 cataaccaag gcacctgtcg gaaaatctgg tccaggcaag acttccataa tctcacgtgt
    35281 tgttacctcg ggattgtcca taactagctt aactgcctcg atagtctcac ccaagttgtg
    35341 tggaggaatg ttggtagcca taccaacggc gataccagtc gttccgttaa ccagaaggtt
    35401 agggaaacga gcaggtaaaa cctcaggctc acgttcactc gcgtcatagt tatccgcaaa
    35461 atttaccgtg ttcttgttaa tatcacgaag catttcaagc gcaatcttgc tcatgcgtgc
    35521 ttctgtataa cgctgagcgg ctgctccgtc gccatccatg gaaccaaagt ttccgtgacc
    35581 atcaactaac atgtgacggt agctccacca ttgtgccata cgaaccattg cttcgtaaat
    35641 cgcactatct ccatgcgggt gatatttacc cataacatcc cctgtgatgc gggctgattt
    35701 tttatgtggt ttatccggtg taattcctaa ttcattcatc ccatacaaaa tacggcgatg
    35761 aaccggcttc agaccatcac gtacatccgg cagagcacgt gaaacgataa cactcatcgc
    35821 atagtcgatg aaggatgatt tcatctcatt ggtcagatta accgttacta aatttttatc
    35881 ttgcattata tgcctctttc aacagtcatt gtgcttctat tataccatat tggcgaataa
    35941 atttctttgt tggaaactag catgtaaaca ttttcaaagg cgctttcaca agtgacaaaa
    36001 agcttaaaac atgctagaat gtattacggt cgggatgaag tcatccccaa ataaaactaa
    36061 ggagatgttt agcaatgact gcaactaaac aacacaaaaa agtaatcctt gtcggtgacg
    36121 gtgccgtagg ttctgcttat gcttatgctc ttgttaacca agggattggt caagaattgg
    36181 gtatcatcga tatcaacaaa gaccgtactc aaggtgatgc agaagacttg agccacgcat
    36241 tggccttcac attccctaaa aaaatctact ctgctgagta ttcagatgct catgacgcag
    36301 acttggttgt attgacagct ggtttgccac aaaaaccagg tgaaactcgc cttgaattgg
    36361 tagagaaaaa ccttcgtatc aaccaacaaa tcgttacaga aatcgttaac tctggtttca
    36421 acggtatctt ccttgttgct gctaaccctg ttgacgtatt gacgtactca acttggaaat
    36481 tctctggttt ccctaaagag cgtgttatcg gttctggtac ttctcttgac tcagctcgtt
    36541 tccgtcaggc attggctgag aaaatcggta tcgacgcacg ttcagttcac gcttacatca
    36601 tgggtgagca cggtgactca gaatttgcag tttggtctca tgccaacgtt gccggtgtga
    36661 aattgtacga ctggttgcaa gataaccgcg atattgatga gcaaggtctt gttgacttgt
    36721 ttgtatctgt tcgtgatgca gcctactcaa tcattaacaa aaaaggcgca acttactacg
    36781 gtatcggtgt tgcacttgca cgtatcacta aagcaatctt tgatgatgaa aatgcagtac
    36841 ttccattgtc agtttaccaa gctggccaat acgaaggtgt tgaagatgtc tttatcggtc
    36901 aacctgctat catcggtgcc cacggtatcg ttcgtccagt caatatccca ttgagcgaag
    36961 ctgaattgca aaaaatgcaa gcatctgcta aacaattgaa agacatcatc gatgatgctt
    37021 tcgcaaaccc agaaattgca gctggtgtaa aaaactaatt atataaaata gtaagaaagg
    37081 tgagcaatag ctcg
//
LOCUS       DATUCG010000007        34231 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.007, whole genome shotgun sequence.
ACCESSION   DATUCG010000007 DATUCG010000000
VERSION     DATUCG010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34231
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.007"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(11..256)
                     /locus_tag="VCA20_000288"
     CDS             complement(11..256)
                     /locus_tag="VCA20_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008472796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HEP1834158.1"
                     /translation="MLFLIQATILVIPKKLKSKQVLFAYLQEELAKKGSTFTEKEVNA
                     FLAEVYDDYAILRRYMVDYGYLSRDQYGLEYRIEEKR"
     gene            complement(457..2856)
                     /locus_tag="VCA20_000289"
     CDS             complement(457..2856)
                     /locus_tag="VCA20_000289"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEP1834159.1"
                     /translation="MLVSYKWLKELVDFDATSHDLSEKMSTTGIEVEGVEQKSAGLSK
                     LVVGQVVSAEPIPDTHLNICQVNVGEEITQIVCGAPNVKAGIKVIVALPGARIAGNYK
                     IKKGKIRGVESLGMLCSLSEIGVSDSVVPKVYADGIYHLPEDAVVGEPIFSYLDLDDE
                     IIELSITPNRADALSMRGVAHEVAAIYDSKVNFKPVVLEESDKKASDVIEVAIESDKV
                     TAYTARVIENVTIAPSPQWLQNLLMNAGIRPLNNVVDITNYILLYFGQPMHAFDFDKF
                     DGKKIVARQAKEGENLVTLDGEERDLITDDIVISVADKAVALGGVMGGANTEIDNSSK
                     TVVLEAALFDGKSIRKTSGRLNLRSESSSRFEKGINVATLTEAMDTAAAMIAELAGGQ
                     VLSGIVSAGSVDTSDVPVTATIDYVNRSLGTKLDYAQIADIFRRLGMETTGDASQFTV
                     AVPRRRWDIRIPADLVEEIARIYGYNNLPTTLPKEDGTAGELTLTQNIRRQVRSLAEG
                     AGLTEIISYALTTPEKAVEFVARPTTVTELMWPMTVDRSALRQNMVSGMLETVAYNVA
                     RKNKNLALYEIGKIFEQSGNPKEDLPQEINKFALVLTGLVAEKDFQTPAVPVDFFHAK
                     GILEAIFDHYKLAVDFVATSEIAALHPGRTAEIHLNGAVIGFVGQVHPQTAKDYGIPE
                     TYVTEINLDAIEEALQPAQPFTEISKFPAVSRDIALLLSSDVKHQDVLDAIAGAGVKR
                     LTKVTLFDVYAGSNIEAGKKSMAYNLTFQNPADSLTDEEVAKYMEKISKALVELGAEI
                     R"
     gene            complement(3211..4254)
                     /gene="pheS"
                     /locus_tag="VCA20_000290"
     CDS             complement(3211..4254)
                     /gene="pheS"
                     /locus_tag="VCA20_000290"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEP1834160.1"
                     /translation="MSNIEQQLAELSQTTLEKLKEIQHQGEKELQDLRVAVLGKKGSL
                     TDLLKGLKDLSNEMKPIVGKQVNEVRDVLTAAFEETAQKVAAAKIQQQLASETIDVTL
                     PGRQVKVGKRHVLTQTSEEIEDIFLGMGFQIVDGFEVEKDYYNFERMNLPKDHPARDM
                     QDTFYITEEILMRTHTSPVQARTMDQHDFSKGALKMISPGRVFRRDTDDATHSHQFHQ
                     IEGLVVGENVSMGDLKGTLEMIIKKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGG
                     DGCNVCKKTGWIEILGAGMVHPQVLEMSGIDSTKYSGFAFGLGQERIAMLRYGINDIR
                     GFYQGDVRFSEQF"
     gene            complement(4712..6436)
                     /locus_tag="VCA20_000291"
     CDS             complement(4712..6436)
                     /locus_tag="VCA20_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HEP1834161.1"
                     /translation="MPRLVDLRKKPYCLNDEQITWVENTLAHLTDEEKIGQLFVNLFF
                     FGGDAFSGNNLSNQELMNRYHIGGARYMNGSPEQVQGLINELQSYSKVPLLVAANCDS
                     GGDGAVKGGTYISSGAQSEASRDPRIPYLAGLVSAREETALGVNVNFDPCVDIVQNWR
                     NTIVNTRAYGTNADDVIAYTSAYLEGLTAERDVIQCIKHFPGDGTEERDQHLVLGVNE
                     LSPEEWDKSFGRVYRHHIEAGVEMIMAGHIALPYYQQTLNPSLKDEDILPATLAPELI
                     QDLLKEKLEFNGLVITDASHMLGMTAAMRREDYVPAAIAAGCDMFLFFNNLEEDFEFM
                     LNGYRKGVITEERLHDALRRILGLKAKLNLHVKQAEGTLLKSADELAIIGCEEHLAWQ
                     REAADKAITLLKDTQKNLPISPETHRRIRLYYLEGEKEGIMTASTEVVDNLKAALEAR
                     GYEVTVNDGTTRIKGKTLQYREEVDLALTIANVIGYGAQNNYRIQWKTAMSNEVPWYV
                     HEVPTVFVSLNYTTHLHDVTMVKTAINAYHHNKNTIESLLDKLEGKNDFYGVPNENVW
                     ANKWQAKL"
     gene            complement(6447..8237)
                     /locus_tag="VCA20_000292"
     CDS             complement(6447..8237)
                     /locus_tag="VCA20_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HEP1834162.1"
                     /translation="MTHLVDLRKKPYSLDEEAISWIEETIAQMTLDEKIGHLFVNMGG
                     QRTEEYLTGVLNDYKIAAVRYNPGPAADIWEQNYILQTKSKIPLLIAANTESGGNGAV
                     TDGTKIGDEIKIAATADPRYAYEMGKVAGLEAAAVGCNASFAPIMDLSRNWRNPIIAN
                     RTWGTNVDQVIELSKEYMRGIMEHGIVPFAKHFPGDGIDERDHHLSFASNPMTKSEWM
                     ETFGRIYGEMAEAGLPGIMAGHIHLPNVEKEMHPERELDDMLPASLNKTLLDELLRGE
                     LGYNGAIVTDASHMIGMTASMPRRELLPTAIEAGCDLFLFFNDVEEDLQWMKEGLENG
                     LLSEERLHDALRRTLGLKAKLGLHLFEGRRQEIMMPKEEALALIGRDEAKQLAKEVAD
                     KAITLVKAKQEGIFPVNSERYKRILLVEVDGYKGGFGAMINAGKKRAADTLKELLEAR
                     GHQVSIWENTEARIAKLPEEERPVAIANVYASKRPIAEITDNYDLIINLVDVNSGGTT
                     QRIIWPAAKGTPDQPFYVHEIPTIVVSVQHPFALADMPQVATYINAYDGLPVTLEALV
                     AKLAGESAFIGVSPVDAYCGLVDTHIWRGK"
     gene            complement(8284..9084)
                     /locus_tag="VCA20_000293"
     CDS             complement(8284..9084)
                     /locus_tag="VCA20_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEP1834163.1"
                     /translation="MMKTDYVFCVDSDGCAMDTMTYKHKLFFGPLAADVFDVKDKEPF
                     LAEWNRVNLYSRERGINRFVGLVKGLEFAGVTGIDNLKNWVATTDSLSNASLERLIEE
                     QPSKDLELALKWSTQVNQAIKQYSGPVLAFIGVHKGLEKLSQLGKVYVVSSANKEAVE
                     EEWTDQGLMDFVTELYCQDRGKKEDVIKLLIEEGYCPDKIMMIGDSPGDLKAAELNGV
                     HFYPILVGREMQSWADLTETIADECVHQAFTGEKETELIQAFWNNLDD"
     gene            complement(9084..9923)
                     /locus_tag="VCA20_000294"
     CDS             complement(9084..9923)
                     /locus_tag="VCA20_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEP1834164.1"
                     /translation="MSRVIEFKDKVVVITGAGGVLCGYLAKEFAKAGAKVALLDLNEA
                     AAQVFVDEIVAAEGVAKAYKSNVLSKENLEEVRQQVLADFGPVDILINGAGGNNPKAT
                     TDNEFHEIGLPAETKTFFDLDEGGINFVFNLNYLGTLLPTQVFAQDMIGRSGANIINI
                     SSMNAFTPLTKIPAYSGAKAAISNFTQWLAVHFSKVGIRCNAIAPGFLVTNQNRGLLF
                     DESGQPTARANKILTNTPMGRFGEAEELVGGVFFLADENLASFVNGVVLPIDGGFSAY
                     SGV"
     gene            complement(9925..11025)
                     /locus_tag="VCA20_000295"
     CDS             complement(9925..11025)
                     /locus_tag="VCA20_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEP1834165.1"
                     /translation="MKMSFRWYGKKDPVTLEEIKAIPGMQGIVTAVYDVPVGQAWPLE
                     NILELKKMVEEAGLEITVIESIPVHEDIKQGKPNRDALIENYKTSIRNVGAAGIPVVC
                     YNFMPVFDWTRSDLHHPLPDGSTSLAFLKSDLAGVDPVADDLNLPGWDSSYSKEEMKA
                     IIENYRQNISEEDLWANLEYFIKAILPTAEEAGVKMAIHPDDPPYGIFGLPRIITGQE
                     AVERFLNLYDSEHNGITMCVGSYASDPKNDVLAMTEYALKRNRINFMHTRNVTAGAWG
                     FQETAHLSQAGDIDMNAVVKLLVDYDWTGALRPDHGRRIWGDQTKTPGYGLYDRALGA
                     TYFNGLYEANMRAAGKTPDFGITVKTVGNKES"
     gene            complement(11039..12439)
                     /gene="uxaC"
                     /locus_tag="VCA20_000296"
     CDS             complement(11039..12439)
                     /gene="uxaC"
                     /locus_tag="VCA20_000296"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEP1834166.1"
                     /translation="MSFNDKNFMLKNEPAKELYGKIAELPIYDFHCHLDPKEIFEDKV
                     YEDIVDLWLGGDHYKWRLMRANGISEEEITGSASKLDKFKAWARTLERAFGNPLYHWS
                     HLELRQVFGVEELLTEGNAERLYHQLNAYLQEHQVSPRKLIADARVTFIGTTDHPLDS
                     LEWHKRLAEDSTIDTVVAPSFRPDEAFIEHRNFNPFIKRLAEVTGVAVRDFASFVEAL
                     GQRVSYFAQYGCRASDISFTTISYEEASLEELDDILLARLGGKEAGQSSINKWQTAIF
                     RELCRLYKKHGFVTQVHFGALRNNHTGLFEKLGADVGVDSIGDQTCLTVNLNRLLDNL
                     VKENVLPKMIWYNLNPSYNIALANTLANFQSNEEGLRSQLQFGAGWWFNDTKLGMIDQ
                     MNAYAEQGMLANFVGMLTDSRSFLSYQRHDYFRRILATYVGQWIVDEEVPEDYNRLGR
                     FVEAISYYNAKEFFEQ"
     gene            complement(12441..13061)
                     /gene="eda"
                     /locus_tag="VCA20_000297"
     CDS             complement(12441..13061)
                     /gene="eda"
                     /locus_tag="VCA20_000297"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEP1834167.1"
                     /translation="MLKQLKENYFFAVIRGKDEKDAKEIARHAILGGIRNIEITFSTP
                     NAATVITELQEEFSDDSSVVIGAGTVMNLKLAQAAIDAGASFLVSPHFDKEIQDLAQE
                     AEVFYFPGCATATEIVTASQAGCPIIKLFPGGVLGPGFIKDIHGPVPQVDLMPSGGVS
                     VENVADWKKAGACAVGVGSALASRVQAEGYESVTTIARSFVAALEG"
     gene            complement(13186..13902)
                     /locus_tag="VCA20_000298"
     CDS             complement(13186..13902)
                     /locus_tag="VCA20_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HEP1834168.1"
                     /translation="MAKPLVEKASERLLELILERGYEVGDKLPNEYELAQDLEVGRST
                     VREAVRSLATRNILEVRQGSGTYISSKKGVSEDPLGFSLVRDRVKLTTDLFEVRYLLE
                     PRIAERVAQFASDEDVARLEEIVLAIEEAVAAGDTKHLELDVKFHCMLAEMSGNLAMT
                     SLMPVINQSIHLINENYSNRQMKSDSLQAHRNILMAIKQRHPVAAYDSMLAHIISVRQ
                     VVLSEWFDKDMTLHGLNDHK"
     gene            complement(13948..15747)
                     /gene="uidA"
                     /locus_tag="VCA20_000299"
     CDS             complement(13948..15747)
                     /gene="uidA"
                     /locus_tag="VCA20_000299"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HEP1834169.1"
                     /translation="MLYPIQTKTRSVYSLNGIWKFKQGNNDVHTLLDTDEVMVVPSSF
                     NDLVVDKDKRRFVGDNWYELMVALPVVADDEELVVRFGSVTHQAKVYADGQLIGEHKG
                     GFTPFECLIPSNLYDADQFRLTVCANNELNYTTLPVGNYSEEMDENGQIVKTVKENFD
                     FFNYAGIQRTVHLYKRPKNRIEDIVIRTELNQDLTQAVVNVDVKTVGQYDSIRMSILD
                     QEGQEVGLLENGQMVIEHPRLWEVLDAYLYTAKVELFDGENLLDTYSEQFGIRSVAVE
                     NGQFFINGKPFYFKGFGKHEDTFINGRGFNEAANLMDLNLLKDIGANSFRTSHYPYSE
                     EMMRLADRLGIVVLDEVPAVGLFQLFNAALNLTGDSEEAKNTWEVMQTREAHELVIDE
                     LIARDKNHPSVVMWVVANEPAGHEKGARAYFEPLVQRMRDRDPSKRPVTLVNIMTATP
                     DKDEVMDLVDVVCLNRYYGWYVEHGDLKAAEKGLRQELETWQGLYPDKPILITEYGAD
                     TLPGLHSMWDIPYTEEFQVDYYDMNHRVFDSIPNLVGEQVWNFADFETTVGIIRIQGN
                     HKGLFSRNRQPKQIVREIKKRWTAIPNYHYKRK"
     gene            complement(15763..16791)
                     /locus_tag="VCA20_000300"
     CDS             complement(15763..16791)
                     /locus_tag="VCA20_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEP1834170.1"
                     /translation="MKKILAFGEVLLRLSPPHYQTLTQATSLDCQFGGSELNVLASLA
                     QLSHSVELVTALPKNEVGKMAEHFLFARQIGQKYVIRKEGRLGLYYYQKGFSLRPSQV
                     TYDRDYSAFSLSKEEDYDFEGMFDGVDWFHVSGITIALNPDIYRLAFRLMEMAKGQGV
                     KVSFDLNYRESLWSSFEEAREKLSPFVGLADICFGLEPIQLFNEFGKDVKDELGLKRP
                     YENKEVLLAVVKGLAETYSLQSLAFTQREMTYNNEYLLKAYLYQDGILYETEKESIQV
                     LDRVGTGDAFTAGIILGYLQEKTPREILDLGMASFQFKHTIEGDVNIISQSDIDLLLK
                     KGSREIKR"
     gene            complement(16862..18400)
                     /locus_tag="VCA20_000301"
     CDS             complement(16862..18400)
                     /locus_tag="VCA20_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEP1834171.1"
                     /translation="MEKHVEQRVEDVTYNKAKLWQIILFAMNNSSTNIYLVAFTFVTY
                     LSTGVLGLAALFVSQLMGYIRIFDGFIDPTIGVIIDKTDTKFGKYRPILVLGNIITAA
                     SLFMMLGLSGVSESLRFPLFILVLIIHKIGYSLQQTITKAGQVALTNDPKQRPIFNIV
                     DGIVSTVLMTGSQVVVSGTLVPKHGGFTPSFFQELFLGVVVISAVLGILAIIGIWEKD
                     NKKYFGLGEKTQETKLKDYWKVIKGNKPLQVLSISAAFVKFVVQLFGDSVVGVMLYGI
                     IFGDYALSGKMSMLLILPGILVTVGAAQLARRKGLRFAYITALQLGIAGLMALAAMLF
                     SAKPGMLDLTNWNLYTISFFVVYIIARYASGAPAGLVLTMGADITDYETSVSGRYLSG
                     MIGTIFSLTDSIASSLAPMVVGFVLAGIGFGKEYPTVETAFSTDLRMALIVLLTVIPA
                     VVLSLSLFLMKFYKLDREEMARIQEKIHVMKAAKDEERVRAIAKNVPLSDMDYVDVSQ
                     YKVD"
     gene            complement(18588..19427)
                     /locus_tag="VCA20_000302"
     CDS             complement(18588..19427)
                     /locus_tag="VCA20_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEP1834172.1"
                     /translation="MAKKNVKKQAMSLLSLLATIVVVAGGYLLSDNKSSNVADYSYYV
                     NQSKASEGTPNQELASSVLTEDVKKQLGNSIEWNGAGAFIINGNQTDLDASVASVPYG
                     DNKTKTVQGETVPTVANALLAKSTRQYKDREETGNGRTSWTPAGWHQIHDLTGEFDHA
                     VDRGHLLGYALIGGLSGFDASTSNPKNIAVQTAWSNQANDQTSTGQNYFESQIRRVLD
                     KNKRVRYRVTLVYAESEDLVPVGSHLEAKAADGSLEFNVFVPNVQSGLSINYHSGEIA
                     VNQ"
     gene            complement(19457..19648)
                     /locus_tag="VCA20_000303"
     CDS             complement(19457..19648)
                     /locus_tag="VCA20_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEP1834173.1"
                     /translation="MDKDNLSYVGKNLILVAVVLLIAILVFILGLMVGYGVIGDGDNV
                     FAVLSPAKWQELIGKFTGK"
     gene            complement(19641..20924)
                     /gene="murA"
                     /locus_tag="VCA20_000304"
     CDS             complement(19641..20924)
                     /gene="murA"
                     /locus_tag="VCA20_000304"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEP1834174.1"
                     /translation="MDTIIVTGSKNGLKGQVEIEGAKNAVLPLLAATVLASQGQTKLT
                     QVPILSDVYTMNNVVRGLGVRVKFDEETKTIDIDATGKLGSETPYKYVSQMRASIVIL
                     GPILARNGYAKVSMPGGCTIGSRPINLHLKGLQAMGAKIKQRAGYIEASAENLQGAHI
                     YLDFPSVGATQNLMMAATLAQGTTVLENAAREPEIVDLAIFLNKIGAKVKGAGTETIT
                     ITGVEELTGAEHQVVQDRIEAGTFMVAAAMTGGDVLVKDAVWEHNRPLISKMLEMGVE
                     VTEEAEGIRVRSQVDKLKPVTVKTLPHPGFPTDMQAQFTALMAVAKGESTMIETVFEN
                     RFQHLEEMRRMDLHSEILRDTAIITGGLALQGAQVMSTDLRASAALILAGLVAEGETI
                     VGKLTHLDRGYYRFHEKLAALGATIERVSGEYENG"
     gene            complement(20949..21173)
                     /locus_tag="VCA20_000305"
     CDS             complement(20949..21173)
                     /locus_tag="VCA20_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEP1834175.1"
                     /translation="MVISILQFFSHILFIYLAHHLLVTTVDWSRWLKVTGDNQRKINL
                     LILFLAIALGYLVSTFFLELLMMGRFFANM"
     gene            complement(21233..21649)
                     /locus_tag="VCA20_000306"
     CDS             complement(21233..21649)
                     /locus_tag="VCA20_000306"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEP1834176.1"
                     /translation="MGQMTVQIVTPDGIKYDHHAAFVLVKTVDGEMGVYSGHEELIAV
                     LEIGEMKVRRVDDENHVDWIAVNGGIIEVNKDIITVISDSAERERDIDISRAERAKLR
                     AERELEEAQTARNIDMEMRATVALQRAINRIRVGKH"
     gene            complement(21659..23065)
                     /gene="atpD"
                     /locus_tag="VCA20_000307"
     CDS             complement(21659..23065)
                     /gene="atpD"
                     /locus_tag="VCA20_000307"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEP1834177.1"
                     /translation="MSSGKITQVVGPVVDVAFAAEDKLPEINNALVVYKNDDSKQKVV
                     LEVALELGDGVVRTIAMESTDGLTRGMEVLDTGRPISVPVGKETLGRVFNVLGDTIDL
                     EESFPADFEREPIHKKAPAFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLTQ
                     LGIYPAVDPLASSSRALAPQIVGEEHYAVAMEVKRVLQRYQELQDIIAILGMDELSDE
                     EKTLVGRARRIQFFLSQNFNVAEQFTGMPGSYVPVAETVKGFKEILDGKHDHLPEDAF
                     RNVGSIEDVVAKAAKMKF"
     gene            complement(23085..23963)
                     /locus_tag="VCA20_000308"
     CDS             complement(23085..23963)
                     /locus_tag="VCA20_000308"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEP1834178.1"
                     /translation="MAGSLNEIKSKIASTKKTSQITGAMQMVSASKLAKSEQLAQSFQ
                     IYASKVRQITTDLLRGELMASDTSNPMLIRRPVQKSGYIVITSDSGLKGSYNSSILKA
                     VMGMIEQDHDSKDEYEIIAIGSMGADFFRARGINPVFELRGLADNPSFEEVQKIISKS
                     VEMYKNELFDELYVCYNHHVNSLTSQVRVQQMLPVEDLDHNEADGYTATFELEPNREV
                     ILEQLLTQYAESTIYGAILDAKTAENAAGMTAMQTATDNAKNVINDLTIQYNRARQAA
                     ITQEITEIVAGASALE"
     gene            complement(23975..25495)
                     /locus_tag="VCA20_000309"
     CDS             complement(23975..25495)
                     /locus_tag="VCA20_000309"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEP1834179.1"
                     /translation="MESGVLLINAQEISALLKQQIEGFQPDFDYTETGVVTYIGDGIA
                     RAQGLDNAMSGELLVFENGTIGMAQNLETNDVGIIILGQFTDIREGSVVRRTGKIMEV
                     PVGSALIGRVINPLGQPVDGLGEIRTSKTRPIEYPAPGVMQRKSVNEPLQTGLKAIDA
                     LVPIGRGQRELIIGDRQTGKTSVAIDAILNQKGQDMICIYVAIGQKESTVRTQVETLR
                     QYGALDYTIVVTASASQPSPLLFLAPYAGVAMAEEFMYEGKHVLIVYDDLSKQAVAYR
                     ELSLLLRRPPGREAYPGDVFYLHSRLLERSAKVSDELGGGSITALPFIETQAGDISAY
                     IATNVISITDGQIFLKDDLFNSGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRIDLA
                     SYRELEAFTQFGSDLDAATQAKLNRGRRTVEVLKQPLHKPLPVEKQVLILYALTNGFL
                     DSVPIDDILAFEEELYAYFDLHYDGLLDVIRTTKDLPDTDELNAAIQAFKDQSVFK"
     gene            complement(25499..26032)
                     /locus_tag="VCA20_000310"
     CDS             complement(25499..26032)
                     /locus_tag="VCA20_000310"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEP1834180.1"
                     /translation="MNARENAIVQKYALSFVEKVSDHADIWDMYDQISDLISIIHDSK
                     LNRILLSATVSREEKADFVRTVRQSSFWQINDLIEDVIRDGHADLLLETLERVQLQIS
                     KFKNEFEARVVSVYPLTEAQKERLRHLVEQRFSLRVRNITEELDQSLLGGFIVTVNHK
                     VIDASVRTQLKDVRKKL"
     gene            complement(26033..26539)
                     /gene="atpF"
                     /locus_tag="VCA20_000311"
     CDS             complement(26033..26539)
                     /gene="atpF"
                     /locus_tag="VCA20_000311"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEP1834181.1"
                     /translation="MATTITMQSSTILGNFILVTASFAVLIILIRVFAWDKITGIFEE
                     RANKIANDIDAAEEKLTAAANLVQQREDELVQGRIESQKIIQDAVERAKLEKKRILEQ
                     ADVEIQGLKQKAQLEIEAEKREAQENLRVQVAELAVDLASKIILEDLDQQAHSNLIDR
                     YLDKLGDK"
     gene            complement(26557..27273)
                     /gene="atpB"
                     /locus_tag="VCA20_000312"
     CDS             complement(26557..27273)
                     /gene="atpB"
                     /locus_tag="VCA20_000312"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEP1834182.1"
                     /translation="MEEHLSPTLTLGPVTFDLTMVLVSVITISIIFLLVFWASRRMEL
                     KPKGKQNVLEYVYELTINFTKGNLGDAEAKRYSLFFFTVFTFLLVANNLGLMTKLETA
                     EGHNLWTSPTANMAYDFGLATIATVFCHVEGIRRRGFKAYLKSFVTPWAMAPMNILEE
                     VTNLVSLALRLYGNIYAGEVLVSLLLQLSQQSALAYPIAFALNVVWTAFSVFISCLQG
                     YVFIMLVSMYLNKKISSESE"
     gene            complement(27310..27510)
                     /locus_tag="VCA20_000313"
     CDS             complement(27310..27510)
                     /locus_tag="VCA20_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEP1834183.1"
                     /translation="MSLGALALGLACLGVSIGEGLLVASYLSSTARQPEMQSKLMAGV
                     FLGVAFIEGTFFVTLAMTFVLK"
     gene            complement(27647..28690)
                     /locus_tag="VCA20_000314"
     CDS             complement(27647..28690)
                     /locus_tag="VCA20_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEP1834184.1"
                     /translation="MEERKRARLIYNPTSGQEIMKKNVAEVLEILEGYGYETSAFQTT
                     AEKDSAKNEATRAALAGFDLIIAAGGDGTINEVVNGIAPLEKRPQMAIIPTGTTNDYA
                     RALKVPRGNPVEAAKVIGKQQTILMDIGLAKNQKNGFHQEHYFINIAAAGTLTELTYS
                     VPSQLKTMFGYLAYVVKGAELLPQVQFTPVRVEHDEGVFEGSVSMIFVALTNSIGGFE
                     QIVPDAKLDDGNFTLLMVKTGNLFEILHLIRQVLDGGKHIESDLVEYIKTKSLSIENL
                     NPDNRLLLNLDGEFGGEAPVRLYNLSNHIEFFADTDLVSDHAITLDTDQMNREDMAKR
                     FIEETDHIDEAIK"
     gene            complement(28701..30659)
                     /gene="ligA"
                     /locus_tag="VCA20_000315"
     CDS             complement(28701..30659)
                     /gene="ligA"
                     /locus_tag="VCA20_000315"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEP1834185.1"
                     /translation="MKTRISELVSVLNQYSKEYYQLDQPSVSDAEYDTLYRELVELET
                     AHPELILPDSPTHRVGGKVLDGFEKYSHVYPLFSLQDAFSREELEAFDQRVRKEFPQA
                     TYICELKIDGLSISLTYEAGNLVVGATRGDGSVGENITENLKRVADIPLTLPEAADIT
                     VRGECYMPKASFDRVNKQRQEAGEAEFANPRNAAAGTLRQLDTGVVAQRGLATFLYQE
                     ATPSEVTSQSQVLEKLDALGFVTNHEYCLAESIDDTWDFIEKIAERRDDLPYEIDGVV
                     IKVNDLAIQEELGFTVKAPRWAVAYKFPAEEKEAEILSVDWTVGRTGVVTPTANLSPV
                     QLAGTTVSRATLHNVDYIAEKDIRIGDTVIVYKAGDIIPAVLKVVDKYRKEQEIMPIP
                     SHCPSCQSDLQHYEDEVALRCINPICPSQLMSKLEHFASRDAMNIAGLGSSIVEKLFG
                     AGLVHDVADIYKLTVDDLLTLEGFKEKSADKLHQAIQTSKSNSAERLLFGLGIRHVGS
                     KASKILVEKFGDLETLAFADQEAIASLEGLGQVIAKSLTTFFASEGAQQLLAELKEAK
                     VNLTYLGQVVDENAALSGMTVVLTGKLERMKRNEAKAKLEALGANVAGSVSKKTNLVV
                     AGTDAGSKLTKAQELGIEIKDEAWLESL"
     gene            30782..30928
                     /locus_tag="VCA20_000316"
     CDS             30782..30928
                     /locus_tag="VCA20_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834186.1"
                     /translation="MAVTYKRQDDLEKMLEEFASFEKLEEIEYPDPKSKENSGKNNLN
                     QDKK"
     gene            30930..31835
                     /locus_tag="VCA20_000317"
     CDS             30930..31835
                     /locus_tag="VCA20_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEP1834187.1"
                     /translation="MFGLDQLPTSVLQAGAIFLSIMIEALPFVLIGAIISGFIDVFIT
                     PDKVRKLLPKNKFLAILFGTFIGFIFPSCECGIVPIVNRLLEKKVPSYTAVPFLVTAP
                     VINPIVLFATFTAFGNSLLFALYRALGAIIVSLVLGIILGFFVKGPILKGSKFDHHQH
                     DYSDKTSWQKVGFALIHAIDEFFDTGRYLVFGCLFASLVQVYIPTAVLTTIGHSPLTA
                     ILLMMLLAFLLSLCSEADAFIGASLLSSFGFAPVMAFLVIGPMVDVKNLLMMKHYFKG
                     KFIAGFIITITLVILGYSLILGGVV"
     gene            31832..32647
                     /locus_tag="VCA20_000318"
     CDS             31832..32647
                     /locus_tag="VCA20_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEP1834188.1"
                     /translation="MIRFLILAGYFEMTMYLYVSGKLDQYINLHYSYLAYLSMILSFI
                     LAIVQLYVWVKEIRVHSHLKSKIAKLSSIGLLLIPLAIAWLFPTVSLDSTTVAAKGYH
                     FPLAAENDTNTQNQEGTTVQYLKPDTSIYFTKSAYQSQMREIANRYLAEETIVVTNEN
                     YMEVMEAIYDYPTEFSGKTIEMIGFVYNDPDNSQQFFLFRFGIIHCIADSGVYGLLST
                     GATTEFPNNTWVKARGTVKVSYHTSLKQNLPTLELQSMVQIEQPDSPYVYRVF"
     gene            32726..34213
                     /locus_tag="VCA20_000319"
     CDS             32726..34213
                     /locus_tag="VCA20_000319"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEP1834189.1"
                     /translation="MSSHVLFTIFGASGDLAKRKLYPSLFRLYKAGHIKENFAVIGTA
                     RRPWTKDFFEQTVIESLGDLPDTPRQAHEFASHFYYQSHDVNDTEHYVALRKLQDNLC
                     EKYNTQHNKVFFLSMAPEFFGTIAKHLKSEQIVDGQGFERLIIEKPFGTSLATATKLN
                     DELAAAFNEDQIYRIDHYLGKEMVQNIFAVRFANIIFEHIWNRDYIDSVQITFAEAIG
                     VEDRGGYYDHSGALKDMVQNHALQVLSLLAMDKPASFQEEDVRAEKIKVFQHLRQPSD
                     EDLKRNFIRGQYAAGSIDGKDYVSYLDEPNIAEASQTETFAGGVFFVDTDRFRDVPFF
                     FRTGKRLTEKGTRVTITFKHAEDIFGQPSEANVLTIFIQPTEGFMLSINGKEVGSHFA
                     LTPAKLNFRHNATALGNSPEAYEKLFFDVLNGDSTNFSHWEEVKASWSLIDRIVDLWA
                     SNQVPLHTYPAGTMGPQAAFDLLESYGHKWVWTPDVWYRERGLLK"
BASE COUNT         9813 a         8134 c         6319 g         9965 t
ORIGIN      
        1 gatgacacaa tcatctcttt tcttctattc tgtattccaa accatactga tcacgcgata
       61 agtagccata atccaccatg tagcgtctca atatagcata atcatcatag acttcagcta
      121 gaaaggcatt gacttctttt tctgtgaagg tagagccttt cttagccaac tcttcttgta
      181 aataggcaaa taaaacttgc ttacttttca acttcttagg aataacaagt atagtggctt
      241 gaattaggaa aaggatagcc ttaagttata gtgttaataa cccactccta aaataacttt
      301 agagttctca aattgacaaa cttagatacg tcccgtttct aattcacttg actataactt
      361 cccatctcga aaaaattttt cttgaatatc ttgagtattc atacttccct ccaaaaaatc
      421 ctagcccgta ggctaggact tgataagatt tacaaattaa cgaatctcag caccaagctc
      481 aaccaaagcc ttactgattt tctccatgta cttagccact tcctcatccg tcaggctatc
      541 cgcaggattt tggaaggtca ggttataagc catagacttt ttaccagcct caatattgct
      601 acccgcatag acatcaaaga gtgtcacttt agtcaagcgt ttcacgcccg caccagcaat
      661 cgcatccaag acatcctgat gcttgacatc gctgctcaag agcagggcaa tatcacggct
      721 aaccgctggg aatttagaga tttcggtaaa tggctgagca ggctggagag cttcttcaat
      781 cgcatccaga ttgatttcag tcacgtaagt ttcaggaata ccgtagtcct tagccgtttg
      841 aggatgtacc tgaccgacaa agccgatcac agccccattc aaatgaattt cagctgtacg
      901 acctgggtgc aaggcagcaa tctcacttgt ggctacaaag tcaactgcca acttgtaatg
      961 atcaaagata gcttccaaaa ttcctttggc atgaaagaaa tcaactggta cagctggtgt
     1021 ctggaagtct ttctcggcaa ccaagccagt caagacaagg gcaaacttat tgatttcttg
     1081 tggcaggtcc tcttttggat tgccagactg ttcaaagatt ttaccgattt cgtaaagagc
     1141 caaattcttg ttcttacgag ccacgttgta ggctactgtt tctagcatac cagacaccat
     1201 attctgacga agggcagagc gatcaacggt catcggccac atgagttcgg tcacggtggt
     1261 tggacgagcc acaaactcca cagccttctc aggtgttgtc agagcgtaag aaatgatttc
     1321 tgtcagacct gcaccttctg ccaggctacg aacttggcga cggatattct gagtcagggt
     1381 tagctcacct gctgttccgt cctctttcgg aagagtggtc ggcagattat tatacccata
     1441 aatacgagcg atttcctcta ccaagtctgc tggaatgcga atgtcccaac ggcgacgcgg
     1501 tactgctact gtgaactgac ttgcatcgcc agttgtttcc atacctaaac gacggaaaat
     1561 atctgcgatt tgtgcatagt cgagcttggt cccgagcgag cggttaacgt aatcaatggt
     1621 tgctgtgact gggacatccg atgtgtccac gctacccgca gaaacgatgc cagacaagac
     1681 ctgaccccca gccagttccg caatcatagc agccgctgtg tccatagcct ccgtcaaggt
     1741 cgctacgttg atgccctttt caaatcgaga agaggactca gatcgaaggt tgaggcgacc
     1801 tgaggtcttg cggatagact tgccgtcaaa gagggccgct tccagcacga cggtcttaga
     1861 gctattgtca atctctgtat tagcaccacc catgacacca ccgagggcaa ctgccttgtc
     1921 cgcaacggaa ataacgatgt catcggtaat caagtcacgc tcttcgccgt caagcgtcac
     1981 caagttttca ccctcttttg cctgacgagc cacgattttc ttgccatcaa acttgtcaaa
     2041 gtcaaaggcg tgcattggct gaccgaagta aagtaagatg tagttggtga tgtccaccac
     2101 gttgttaagc gggcgaatac ctgcgttcat gagcagattt tgcaaccact gtgggcttgg
     2161 tgcgatggtc acattttcaa taacacgggc cgtgtaggca gtcaccttgt ctgactcaat
     2221 agccacttca atcacatcgc tggctttctt gtcgctttcc tccaagacca ctggcttgaa
     2281 gttgaccttg ctgtcataga tagctgccac ttcgtgagcc acaccacgca tagagagggc
     2341 gtcggcacgg tttggcgtga tggacagctc gataatttcg tcgtcaaggt ccaggtatga
     2401 aaagattggc tcacccacga ctgcatcttc gggcaggtgg tagattccat ccgcatagac
     2461 ctttggcaca actgagtcgg acacgccgat ttcgctcaat gagcagagca tacccaagga
     2521 ctcaactccg cggattttgc ctttcttaat cttgtagttg ccagcgatac gagctcctgg
     2581 cagagcaacg attaccttga taccagcttt cacatttgga gcaccgcaaa caatttgcgt
     2641 gatttcttcg cccacattga cctgacagat attgagatga gtatcaggaa ttggctcagc
     2701 agacacaacc tgaccgacaa ccaacttaga caggccagca ctcttttgct caaccccttc
     2761 tacttcaatt cctgttgttg acattttttc agacaagtca tggcttgtcg catcaaagtc
     2821 aacgagttct tttaaccatt tataacttac taacataact tttctttctt tattttttag
     2881 aaaacactac tttttcaact ctaaacctag tgaggggagt aggtagaccg ctagagcggc
     2941 taccctttct ctcaatcaac aatctactaa ttttatcgta ttacgacggt atttcaagcg
     3001 aaacgatatt tttcgcttga aatcctagta aggtagctaa gaaagtcgct taatggcgat
     3061 ttccgttcac ctattcgttc atggttacga cggttcaagg ttgaaaacga cattttttca
     3121 accttagaac tagtgagggg agtaggtagg ccgctagggc agctaccgtc ctgtttctag
     3181 atactttcag tatcagaaac aggtctcaca tcaaaactgc tccgagaatc gtacatcgcc
     3241 ttgataaaat ccacgaatat cgttgattcc gtagcggagc atggcaatac gttcctgacc
     3301 aagaccgaag gcaaagccag agtacttggt tgagtcaatg ccactcattt ccaatacttg
     3361 cgggtggacc atgccggcac cgaggatttc aatccagcct gttttcttac atacgttaca
     3421 gccgtcacca ccgcacttga agcaggaaac atccacctca acagatggct ctgtgaatgg
     3481 gaagtatgat ggacgcaggc gaatctgacg ctcttcacca aacattttct tgataatcat
     3541 ttcaagcgta cctttgagat cgcccatgga tacattttca ccaacaacca aaccttcaat
     3601 ctggtggaac tggtggctgt gggtcgcatc atccgtatca cgacgaaaaa cacgtccagg
     3661 cgaaatcatc ttcaaggcac cttttgagaa atcatgctgg tccatggtac gcgcctgaac
     3721 aggcgacgta tgggtccgca taaggatttc ttctgtgatg tagaaggtat cctgcatatc
     3781 acgtgctggg tgatcttttg gcaaattcat gcgctcaaag ttgtagtagt ctttctccac
     3841 ttcaaaccca tcaacgattt ggaagcccat gcccaagaaa atatcttcga tttcttcaga
     3901 agtttgggtc aagacatggc gttttccgac cttaacctga cgacctggca aggtcacatc
     3961 aatggtttct gaagctaatt gctgttgaat ttttgcggct gcaaccttct gagctgtctc
     4021 ttcaaaggca gctgtcagca catcacgcac ttcattgacc tgtttaccaa cgattggttt
     4081 catttcgtta gataggtctt ttaaaccttt caacaagtca gtcaaggacc cttttttacc
     4141 caaaaccgca acacgcaggt cttgtagttc tttttctcct tggtgctgga tttcttttaa
     4201 cttttctagt gtcgtttggc ttaattcagc caactgttgt tcaatgtttg acataattcc
     4261 tccaataaaa aaacgcatga caccactcgt atcggagcgt tgtcacgcgg taccatccgt
     4321 tttcatctgc aaggcagacc ttagttccat gaacctgtat tcacaagtga agagtttgca
     4381 cataagagat ggttttccgc taatcaaggc agttttttga gggaatactg actgtatttt
     4441 gaaaaaaact aacgatgagt agctgaaaaa ctagccttag atgaaagtgc tgaactgtga
     4501 atacaggttc taagtccttg tgtgagtgaa ttcgaccaac tttcactgat tgagcttgca
     4561 gtcacggctc aactccctgg acagcttcca taaggtgtac tagtctcaca gacttctatt
     4621 caattaagaa tattctatca aaaataagaa agtttggcaa taaatatccc tagaaaaact
     4681 ccctctatct aggatagaaa ggagtttagt tttatagttt tgcttgccat ttattggccc
     4741 aaacattctc atttggcaca ccatagaagt cattttttcc ttccaattta tcaaggaggg
     4801 attcaatcgt atttttattg tgatggtagg cattgatagc agttttgacc atggtaacat
     4861 cgtgaaggtg agttgtataa tttagtgaaa cgaaaacggt tggaacttca tgtacatacc
     4921 aagggacctc attggacatg gccgttttcc actggatgcg gtaattattc tgcgctccat
     4981 atccaatgac attggcaata gtcaaggcta aatcgacttc ttctcggtat tgcagcgttt
     5041 ttcctttgat ccgagttgta ccatcattga cggtcacttc ataaccacgc gcttccaagg
     5101 cagctttaag gttatcaacc acttctgtac ttgccgtcat aatcccttct ttttcacctt
     5161 ctaggtaata caatcggatt ctgcgatggg tttctggact gattggaaga tttttctgtg
     5221 tatctttcaa cagcgtaatt gctttatctg ctgcttcacg ttgccaagcc aagtgttctt
     5281 cacagccgat aatagccaat tcatcagctg attttaataa ggttccttct gcttgcttga
     5341 catgaagatt gagtttggct ttcagaccta aaatgcgacg gagagcatca tgaaggcgtt
     5401 cttcagtaat cactcccttg cggtagccat tgagcataaa ttcaaaatct tcttctaagt
     5461 tattgaagaa taagaacata tcgcaacccg cagcaatagc tgcaggtacg tagtcttcac
     5521 gacgcatggc tgctgtcatc cccaacatgt ggctggcatc tgttatgacc aagccattga
     5581 attccagttt ttctttcaat aaatcttgga tcaattcggg agccagcgtt gcaggtaaaa
     5641 tatcttcatc tttcaaactt ggattcaagg tttgttgata gtagggcaag gctatatgac
     5701 ccgccataat catctcaaca ccagcttcaa tatgatgacg atagacacga ccaaaactct
     5761 tatcccattc ttctggagag agctcattga caccgaggac taggtgctga tcacgctctt
     5821 ctgtaccatc tcctgggaaa tgcttgatac attgaataac atctctttca gcagtcaatc
     5881 cttctagata ggcactggta tatgcaataa catcatccgc atttgtgccg taggctctgg
     5941 tattgacaat cgtattgcgc caattctgaa caatgtccac gcaaggatca aagttgacat
     6001 ttacacctaa tgcagtctcc tcgcgcgcag atacgagacc tgctaggtag ggaatacgag
     6061 gatcccgact ggcttccgat tgagcacctg aggaaatata ggttccgcct ttcacagctc
     6121 catctccacc tgagtcacag ttcgctgcaa ccaagagagg tacttttgaa tagctttgca
     6181 actcgttaat cagtccttgt acttgttctg gactgccatt catataacga gcgccaccta
     6241 tgtggtatcg gttcatcaat tcctgattac tcagattatt tccactaaag gcatcccctc
     6301 cgaagaagaa tagattgaca aacaattgac cgattttttc ttcatcagtc agatgagcta
     6361 aggtattttc tacccaggta atctgctcat cattcaaaca atagggtttc ttccgcaaat
     6421 caactaatct aggcattatt tcctccttat tttcctcgcc aaatatgtgt atctaccaaa
     6481 ccacagtagg catctacagg cgatactcct atgaatgctg actctcctgc tagtttggca
     6541 accaaggctt ctaaagtgac aggcaagcca tcgtaggcat tgatataggt tgcaacctgt
     6601 ggcatatctg caagtgcaaa cgggtgctga acagatacaa caatggttgg aatttcgtga
     6661 acatagaaag gctggtctgg tgttcccttg gcagccggcc aaataatccg ctgagttgtt
     6721 cctcctgagt tgacatcgac taaattaata atcaaatcat agttatctgt gatttcggca
     6781 ataggacgct tggaagcata gacatttgca atcgctactg gtctttcttc ttctggcaat
     6841 ttggcaatcc gtgcttctgt attttcccag atagatactt ggtgacctcg agcttctaat
     6901 agttctttca gcgtatcggc tgctctcttc ttaccagcat tgatcatggc gccaaaacca
     6961 cctttatagc catctacttc gaccaacaaa atacgtttgt agcgttctga attgactggg
     7021 aaaattcctt cttgtttggc ttttaccaag gtaatcgcct tgtcagctac ttcttttgca
     7081 agctgtttcg cttcatctct accaatcaaa gccagtgctt cttcttttgg catcatgatt
     7141 tcctgacgac gaccttcaaa caagtgaaga ccgagtttag ctttcaagcc caaggtacga
     7201 cgaagggcat catgtagacg ttcctcagaa agcagaccat tttccaatcc ttctttcatc
     7261 cattgcaaat cttcttctac atcgttgaag aaaaggaaaa gatcacatcc tgcctcaata
     7321 gctgtaggca aaagctcacg acgtggcata gaggctgtca taccgatcat gtgactggca
     7381 tccgttacaa tggcaccatt gtagcctaat tctccacgaa gtaattcatc caagagggtt
     7441 ttattcagtg aagctggcaa catgtcatcc aattcacgtt ctggatgcat ttctttctca
     7501 acatttggca aatgaatatg ccccgccatg atacctggaa gacctgcttc agccatttca
     7561 ccataaatcc gtccaaatgt ttccatccat tcagactttg tcatgggatt cgaagcgaaa
     7621 gagagatgat ggtcacgttc atcaatacca tcgcctggga aatgcttagc aaacggcaca
     7681 atgccatgct ccataatacc tcgcatgtat tctttggata attcaatgac ttggtctaca
     7741 ttagttcccc aagttcggtt tgcaatgatt ggattgcgcc aattacggct gaggtccata
     7801 atcggtgcaa aggaggcatt gcagcctact gcagctgctt caagtcctgc aactttcccc
     7861 atttcataag catagcgggg atcagctgtc gcagcaatct tgatttcatc cccaattttc
     7921 gttccatccg taacagcgcc atttccacca gattcggtat tggctgcaat caaaagagga
     7981 atcttagact tggtctgcaa aatatagttt tgctcccaaa tatctgcagc aggtccagga
     8041 ttgtaacgaa cagcagcaat cttataatca tttaatactc cagtcagata ttcctctgtc
     8101 cgctggccgc ccatgttgac aaagagatga ccgatttttt catccaaggt catctgagca
     8161 atggtttctt caatccaaga aatagcctct tcatcaaggc tgtaaggttt cttccgtaaa
     8221 tctactaaat gtgtcatgtt tacctccaaa aaagctagag ggcatagcgc ctctagccct
     8281 tctctagtcg tctaaattat tccaaaatgc ctgtatcaat tctgtttctt tctcacctgt
     8341 gaatgcttga tgaacacatt catctgcaat cgtttctgtc aaatctgccc aagattgcat
     8401 ctctcgtcct actaaaatag gatagaaatg gacaccgttc aattccgctg ccttcaaatc
     8461 accaggcgag tcaccaatca tcataatctt atcaggacaa tacccttcct ctattaacag
     8521 tttaatgaca tcttctttct tgccacggtc ttgacagtac aattctgtaa caaagtccat
     8581 caaaccttgg tctgtccact cttcttctac tgcttcctta ttagcagaag aaaccacata
     8641 gacttttcct aactggctca atttttccaa gcccttgtga acgccgatga aggctaaaac
     8701 aggaccgcta tactgcttaa tggcttgatt gacttgagta gaccatttta aggctaattc
     8761 caagtctttt gacggctgct cctctatcaa tctttccaga gaagcatttg acaaagaatc
     8821 tgtggtagcg acccaatttt tcaagttgtc tataccagtc acacctgcaa attctaggcc
     8881 tttaactaag ccaacaaaac ggttaatgcc acgttctcgt gagtagagat tgactcgatt
     8941 ccattctgct aaaaacggtt ctttgtcttt aacatcaaaa acatctgcag ccaaggggcc
     9001 gaaaaaaagt ttatgtttgt aggtcatggt gtccatggca cagccatcag agtccacaca
     9061 aaatacgtaa tccgttttca tcatcaaact cctgaatagg ctgagaagcc accgtcaatc
     9121 ggtaaaacaa caccgttgac aaaacttgcc aaattctcat ctgccaagaa gaagacccct
     9181 ccgaccaact cttcggcttc accaaaccgt cccattggtg tattggtcaa aatcttgtta
     9241 gcacgtgcgg tcggttgacc tgactcatca aatagcaaac cacggttttg gttggtaacc
     9301 aagaatccag gcgcaatcgc attacaacgg atgcccactt ttgaaaaatg aactgctaac
     9361 cattgggtaa aattactaat agcagccttg gcacctgaat aggctggaat ttttgtcaaa
     9421 ggagtaaagg cattcatgct ggaaatattg ataatatttg ctcccgagcg accaatcata
     9481 tcctgggcaa agacctgtgt tggcaataag gttcccaaat agttcaaatt gaatacaaag
     9541 ttgatacctc cctcatccaa gtcgaagaaa gttttggtct cagctggcag tccaatctca
     9601 tggaattcat tgtccgttgt cgctttggga ttattgcctc ctgcaccatt gattaaaata
     9661 tctacaggac caaaatctgc taggacttgc tggcgaactt cttctaaatt ttccttagaa
     9721 agaacgttag atttataggc ttttgcaacg ccctctgctg ctacaatctc atcaacgaag
     9781 acctgggccg cagcctcatt caagtccaaa agcgcaacct ttgcacctgc cttagcgaat
     9841 tcttttgcca aatagccaca aagaacgcct cctgcacctg taataacaac aaccttatcc
     9901 ttaaactcaa taacacgtga catattagct ctccttattt ccaactgttt tgactgtaat
     9961 tccaaaatcc ggtgtcttgc ctgctgctcg catattggct tcatagagac cgttaaagta
    10021 ggttgctcca agggctcggt catacagacc ataaccaggt gtcttggtct ggtctcccca
    10081 gatacgacga ccgtggtctg gacgcaaggc accagtccaa tcatagtcta ccaataattt
    10141 aacaacagcg ttcatatcaa tgtcaccagc ctgtgacaga tgtgctgttt cttggaaacc
    10201 ccaagcacct gccgtcacat tgcgcgtatg cataaaattg atgcgattgc gtttgagagc
    10261 atattccgtc atagcaagga catcattttt cggatcagac gcataggatc ctacacacat
    10321 ggtaattcca ttgtgctctg aatcatagag attcaaaaat cgttcaacgg cttcttgacc
    10381 agtaatgatg cgcggtaatc cgaaaattcc atacggaggg tcatcagggt gaatagccat
    10441 tttgactcca gcttcctcag ctgttggcag aatcgctttg atgaaatact ccaaattcgc
    10501 ccataaatct tcttcagaga tattctgacg ataattttcg ataatagcct tcatttcttc
    10561 cttggaatag gacgaatccc aacctggtaa gttcaaatca tccgccacag gatcaacacc
    10621 tgctaaatca gatttaagga aagctaaaga ggttgaacca tctggaagtg ggtggtgtaa
    10681 gtctgaacgg gtccagtcaa acacaggcat gaaattgtag catacaactg gaatacctgc
    10741 agccccgaca ttacggatgg aagttttata attttcgatt aacgcatcac gatttggctt
    10801 cccttgtttg atgtcttcat gaacaggaat ggattcaata accgtaattt caagtcctgc
    10861 ctcttctacc atttttttca gctctagaat gttttcaagg ggccaagctt gaccaactgg
    10921 cacatcatag acagcagtca caattccctg cataccagga atcgccttaa tctcctcaag
    10981 tgtcacagga tcctttttcc cataccatcg aaatgacatt ttcatgattt actctccttt
    11041 attgttcaaa aaattctttg gcattgtaat aggatatggc ttctacaaac cgccctaaac
    11101 gattgtaatc ttcaggcact tcttcatcaa caatccattg accaacatag gtcgctaaaa
    11161 tacgtctgaa ataatcatgc cgttgataag ataaaaagct acgcgaatct gtcaacatac
    11221 caacaaaatt agcaagcatg ccctgttctg cataggcatt catctggtca atcatgccca
    11281 acttggtatc attgaaccac caaccagcac caaattgtag ctgactacga agcccttcct
    11341 cattggactg gaaattcgcc aaagtattgg ctagagcaat gttgtaactt ggattgagat
    11401 tgtaccaaat catttttgga aggacatttt ccttgaccaa gttatctaac aaacgattca
    11461 agttcacagt cagacatgtc tggtctccaa tcgaatccac acctacatct gctccaagtt
    11521 tttcaaacag tccggtatga ttgttccgta aggcaccaaa gtgaacttgt gtcacaaagc
    11581 catgtttctt atacaatcgg cacaattctc taaagatagc agtctgccat ttattgatac
    11641 tggattggcc agcttcttta cctccaagtc ttgctagtaa aatatcatca agctcttcaa
    11701 ggcttgcttc ttcgtatgaa atagtcgtaa aactgatatc acttgctcgg caaccgtatt
    11761 gagcaaaata ggaaacacgc tgaccaagtg cctctacaaa acttgcaaag tctcttactg
    11821 ctacccctgt tacctccgcc aagcgtttaa taaatggatt gaaatttcga tgctcgataa
    11881 aggcttcatc agggcggaaa gacggtgcca ctacagtatc tatggtcgag tcctcagcca
    11941 agcgcttatg ccactccaag ctatctaatg gatggtcagt agtcccaata aaggtcaccc
    12001 tagcatccgc aatcagctta cgtggactaa cttgatgttc ttgcaaataa gcattgagct
    12061 gatgatagag tcgctctgca tttccttcgg tcaacaattc ctcaacaccg aaaacctgtc
    12121 gcaattccaa atgactccaa tgatagagag gatttccgaa agcccgttcc aaggttcttg
    12181 cccaggcttt aaacttatct agtttacttg cagaacctgt aatttcttct tctgagatac
    12241 catttgcccg catcaagcgc cacttataat ggtcaccccc cagccacaga tcaacaatat
    12301 cttcataaac cttatcttca aaaatttcct tcggatctag atgacaatgg aaatcataga
    12361 taggaagctc tgctattttc ccataaagct ccttagcagg ctcgtttttt agcataaagt
    12421 ttttgtcatt aaaactcatt tcatccctcc aaagctgcaa caaaactgcg agcaatcgtc
    12481 gttacacttt catagccttc agcctgcact cgacttgcca gagccgaacc gactccaaca
    12541 gcacaagcac ctgccttttt ccaatctgca acattttcaa ccgaaacgcc accagaaggc
    12601 atcaaatcca cttggggcac agggccgtga atatccttga taaatccagg acccaacacg
    12661 ccaccaggga aaagcttgat aataggacat cccgcctgac tggctgtgac aatctctgtt
    12721 gcagttgcac agcctgggaa gtagaatacc tcagcctctt gcgccaagtc ttgaatttct
    12781 ttatcaaaat gaggactaac caggaaacta gcacctgcat caatggccgc ttgtgccagc
    12841 ttaagattca tcactgttcc cgccccgatg actacggaag aatcgtctga gaactcctct
    12901 tgtaattctg tgataactgt cgctgcattt ggagtcgaaa atgtaatttc aatatttcga
    12961 atacctccca aaatcgcatg gcgagcgatt tctttcgcat ctttttcatc ttttccgcga
    13021 atcactgcga aaaagtaatt ttcttttaat tgctttaaca tcttgacctc caattagaaa
    13081 gcgttattat ttatagctat agtatacaat tcatcagatg atttgtcaat aaaaaaagca
    13141 aaaaataccc agaagaaatt cttctgggct gtctattttt tatttctatt tatgatcatt
    13201 gagaccatgc aaggtcatat ctttatcgaa ccattcgctt aaaacaacct gtcttactga
    13261 tataatatgt gctaacatgc tatcatatgc tgcaacagga tggcgttgct tgatagccat
    13321 taagatgttc ctgtgggcct gtaaactatc tgatttcatc tgacgatttg aataattttc
    13381 attgatcaga tgaatagatt gattaatgac gggcatgaga cttgtcatag ccagattccc
    13441 actcatctca gccagcatac agtggaattt cacatccaac tccagatgct tcgtgtctcc
    13501 agcagcaacg gcctcttcaa tagcaagaac aatttcttct agtcgagcaa cgtcttcgtc
    13561 cgaggcaaat tgagcgacac gctccgcaat tctcggttca agcaaatacc gcacttcaaa
    13621 caaatctgtc gtcaacttca cacggtctct taccaaagaa aaacctaagg gatcctctga
    13681 cactcctttt ttagagctga tataggttcc tgacccttgg cgcacctcca gaatatttcg
    13741 agtagccaaa cttcgaacag cttcacgaac tgtcgaacgt ccaacttcta aatcttgggc
    13801 caattcatac tcatttggaa gcttgtcacc aacttcatat cctctttcca aaatcaattc
    13861 caataagcgt tccgatgctt tttctaccaa gggtttcgcc atgtgcttgg tcctccatta
    13921 caactttcta tttgctacta ttatagctca tttcctctta taatggtagt ttggaatggc
    13981 agtccaacgt ttcttgattt ctctaacaat ttgcttgggt tgtctatttc ttgagaataa
    14041 tcctttgtga tttccttgaa ttcgaatgat accgactgta gtttcaaaat cagcaaaatt
    14101 ccatacctgc tcaccaacta aattaggaat agaatcaaag acgcgatggt tcatatcgta
    14161 gtaatccacc tgaaattcct ctgtataagg aatatcccac atcgaatgca aacctggaag
    14221 agtatctgca ccatattccg taatcaaaat tggcttatct ggataaagtc cttgccaggt
    14281 ctctaattcc tgtctcaagc ccttttctgc tgccttcaag tcaccgtgtt caacatacca
    14341 accataataa cgattgagac agacaacatc gaccaaatcc ataacctcat ccttatcagg
    14401 cgttgctgtc ataatattga ccaaggtcac gggacgctta gaaggatctc tgtctctcat
    14461 gcgctgaacc aagggttcaa aataagcacg cgcgcctttt tcatgccctg ctggctcatt
    14521 ggcgacaacc cacatcacta cagaaggatg attcttatca cgcgcaatca attcatcaat
    14581 caccaactca tgagcctccc tggtttgcat aacttcccaa gtgttttttg cttcttcaga
    14641 atcacctgtc aaattcaagg ctgcattgaa aagctggaac aggccaactg ctggcacttc
    14701 atcaaggacg acaattccca aacgatctgc tagtcgcatc atttcttcag aatagggata
    14761 atgggatgtc ctaaaggaat ttgctccaat atctttaagc aaattcaaat ccatcagatt
    14821 ggctgcttca ttgaaaccac gaccattgat gaaggtatct tcatgtttac caaatccctt
    14881 gaaatagaaa ggttttccat tgatgaaaaa ctgcccattt tcaaccgcaa cactacgaat
    14941 gccgaattgt tctgagtagg tatctagcaa gttttctcca tcaaataatt caactttagc
    15001 agtgtagaga taggcatcca agacctccca gaggcgtgga tgttcaatga ccatttgtcc
    15061 attttcaaga agacctactt cctgtccctc ttgatctaaa atagacatac ggatggaatc
    15121 gtactgccca actgtcttca catcaacatt cactaccgcc tgagtcaaat cttgattcag
    15181 ctccgtccga ataacaatgt cctcaatcct attttttgga cgtttataga gatgaactgt
    15241 ccgctggata ccagcatagt tgaaaaagtc aaaattttcc ttgacggtct tcacaatttg
    15301 accattctca tccatttctt cgctataatt ccctactgga agggttgtgt aattcaactc
    15361 attattggca cagactgtca aacggaattg gtcagcatcg tataagtttg atggaatcaa
    15421 gcattcaaaa ggggtaaaac cacccttgtg ttccccgatt aactgaccat ctgcataaac
    15481 tttggcctga tgtgtcaccg aaccaaagcg tacaactaac tcttcatcat cagctactac
    15541 tggaagagct accataagtt cataccaatt atctccgaca aaacgacgtt tgtccttatc
    15601 aacaacaaga tcattaaaag aactcggtac aaccattact tcatctgtat ctaacagagt
    15661 atgaacatca ttgttccctt gcttaaattt ccatattcca tttaaagaat aaactgaacg
    15721 tgtttttgtt tgaattggat ataacatact ttttccttct ttctatcgtt ttatttcacg
    15781 tgaccctttt ttcaataaca aatcaatatc tgattgactg atgatattga catccccttc
    15841 aatggtatgt ttgaattgaa agctagccat acctaagtcg agtatttccc gtggcgtttt
    15901 ttcctgcaaa tagcccaaaa taatccctgc tgtaaaggca tcccctgtcc caactcggtc
    15961 caaaacttga atggattctt tttccgtttc atatagaata ccatcttgat ataagtatgc
    16021 cttcagcaaa tattcattat tgtaggtcat ttccctctgg gtaaaagcaa gactttgcaa
    16081 agaatacgtc tccgccagac ctttaaccac cgccaacaag acttctttat tttcataagg
    16141 gcgcttcaac cccaattcat ccttcacatc ttttccaaat tcattgaaga gttgaatagg
    16201 ctctagacca aagcaaatat ctgctaaacc tacaaaagga gataattttt ccctcgcttc
    16261 ttcaaaagaa gaccacaaac tttctcgata attcaagtca aaggaaacct taacaccctg
    16321 cccctttgcc atttccatca aacgaaaggc cagtcgataa atatctggat tcaaagcgat
    16381 cgttatacca ctcacatgaa accaatcgac accgtcaaac ataccttcaa aatcataatc
    16441 ttcttccttg gataagctga aagcagaata atcacgatca taggtcacct ggctaggtct
    16501 aagagagaaa cccttttgat aataataaag ccccagcctt ccctcttttc gaatcacata
    16561 tttctgtcca atttgccttg caaaaagaaa atgttctgcc atttttccca cttcattctt
    16621 aggcagtgcc gtcaccaact cgactgagtg actaagctga gccaagctgg ctaaaacatt
    16681 caactccgaa ccaccaaact gacaatctaa actcgtggcc tgtgttaagg tctgataatg
    16741 aggaggagat agccgcaaca aaacttctcc aaatgctaag atttttttca tactgcctcc
    16801 taggtgaaag acccagctgg aatcctccaa ctaggtcgat actgtattat ttatcgtcgt
    16861 attagtcaac cttatattga cttacatcaa cataatccat atcagataat ggaacatttt
    16921 tcgcaattgc acgcacacgt tcctcatctt ttgccgcttt cataacatgg attttctctt
    16981 gaatacgagc catttcttca cggtctagtt tgtagaattt catcaagaac aaggataggc
    17041 tcaaaaccac tgctggaatc actgtcaata ggacaatcaa tgccattctc aaatctgttg
    17101 agaatgctgt ttcaacggtt gggtactctt taccaaaacc gatacctgct aatacaaacc
    17161 caacaaccat tggggctaat gaagaagcaa ttgagtctgt caatgagaaa atagtcccaa
    17221 tcatacctga caagtaacga ccagatactg atgtttcata gtctgtaata tctgccccca
    17281 ttgtcaatac caatccggct ggagcacctg atgcatagcg tgcgatgata tagacaacaa
    17341 agaaactgat tgtgtagaga ttccaattcg tcaaatccaa catacctggt ttagctgaaa
    17401 agagcattgc tgccaaagcc ataagaccag caattccaag ttgaagcgct gtaatatagg
    17461 caaaacgtaa acctttacga cgagccaatt gcgcagcacc aactgttaca agaatacctg
    17521 gcaaaatcaa cagcatagac attttaccag aaagagcata gtcaccgaag ataataccat
    17581 acagcatcac tccaacaact gaatctccga acagttgcac aacaaatttc acaaaggctg
    17641 cagagattga aagaacctgc aaaggtttgt ttcctttgat aactttccag tagtctttca
    17701 acttagtttc ttgtgtcttc tcaccaagac caaagtattt cttattgtct ttctcccaaa
    17761 taccgataat agcaagaata cccagaacag ctgaaattac tactacaccg aggaacaatt
    17821 cttggaagaa acttggagtg aaaccgccat gtttcggaac caatgtccct gacacaacaa
    17881 cctgactacc agtcatcaac acagttgaca caataccatc aacaatatta aagattggac
    17941 gttgctttgg atcattggta agcgcaactt gtcctgcttt tgtaatcgtc tgttgaagtg
    18001 aatatccaat tttatggata atcaaaacaa ggatgaatag aggaaaacgg agactctcgc
    18061 taactccact taaacctaac atcataaaaa gagaagctgc tgtaatgata ttccccaaaa
    18121 ccaaaatagg acgatattta ccaaacttag tatccgtttt atcaatgata actccaattg
    18181 taggatcgat gaagccatca aagatacgaa tatagcccat gagttgacta acaaacaggg
    18241 ctgcaagacc aagcacacct gtcgataaat aagtcacgaa agtaaaagca accaagtaaa
    18301 tattcgtcga tgaattgttc atcgcaaata atatgatttg ccataacttg gctttgttat
    18361 aagtaacgtc ttcgacacgt tgctcaacat gtttttccat tggaaattcc tcctaaatgt
    18421 tttctacgac aaagtcgtaa gcgttttctt taactatatt ctaatattca tctgatgatt
    18481 tgtcaactgt ttttgtaaat atttttaatt atacgcaaaa aaaagacgat tttacgtgaa
    18541 aatcgtctga tatatttgat ttggcgaggg aaaattcgtc tgactattta ttgattcact
    18601 gcaatttccc cactatgata gttgatactg agaccactct gcacatttgg aacgaacaca
    18661 ttgaactcta ggctaccatc tgctgctttt gcctctagat ggcttccgac agggacaagg
    18721 tcttcactct cagcatacac caaggtcaca cgatagcgga cacgcttatt tttatccaag
    18781 acccgccgaa tctgactttc aaaataattc tgcccagttg aagtttggtc gttagcttgg
    18841 ttggaccaag ctgtttgaac tgcaatattc ttaggattgc tggttgaagc atcaaaacca
    18901 gacaatccac caatcaaagc ataacctagt agatgcccac ggtccactgc atggtcaaat
    18961 tctccagtta aatcatggat ctgatgccaa cccgcaggtg tccaagaagt tcgaccattt
    19021 cctgtttctt cacgatcctt atactgacgt gtagatttgg ccaaaagcgc attggctacg
    19081 gttggaaccg tttctccctg gactgttttt gtcttattat caccataagg aacactggca
    19141 accgatgcat ctaaatccgt ttgattaccg ttaataataa atgctccagc gccattccat
    19201 tcaatcgaat ttcccagttg cttttttaca tcttctgtca ataccgaact agccagttcc
    19261 tgattaggtg tgccttcact tgccttagac tgattgacat aataggaata gtcagcaacg
    19321 ttactgctct tgttatcact taaaagatag ccacccgcta ctacaacaat cgtagccaat
    19381 aagctgagca gactcatcgc ttgttttttt acatttttct tcgccatttt ctctccatca
    19441 aaaaagcagg tccagcctac tttcctgtaa atttaccgat tagttcttgc cacttagctg
    19501 gtgacaacac cgcaaataca ttgtctccat ctcctatgac accgtaacca accattaatc
    19561 ctaaaataaa caccaaaata gcaatcaaca aaacaactgc tactaagatt aaatttttac
    19621 caacatagct aagattatcc ttatccattt tcatattctc cactcacacg ttcaatcgtt
    19681 gcacctaaag cagctaattt ctcatgaaaa cgatagtaac ctcgatctaa gtgcgttaat
    19741 ttccctacaa tcgtctcacc ttcagcaacc aaaccagcca gaatcaaagc ggcacttgct
    19801 cgtaaatcag tagacataac ctgagcacct tgcagagcaa gtcctcccgt gatgatagct
    19861 gtatctcgta aaatttccga atgcaaatcc atccgacgca tttcttccaa atgctggaaa
    19921 cgattctcaa ataccgtttc aatcatggta gattcaccct ttgcaactgc cattagagct
    19981 gtaaattgag cctgcatatc tgttgggaag cctgggtgtg gtaaggtttt tactgttaca
    20041 ggtttaagct tatccacttg cgaacgaact cgaatccctt cagcttcttc cgtcacctca
    20101 actcccattt ccaacatctt agaaatgagt ggacggttgt gctcccagac agcatccttg
    20161 accaagacat cgccaccagt cattgccgct gccaccataa aggtacccgc ttcgatacga
    20221 tcttgcacta cttgatgttc tgcgccagtc aattcttcta caccagtaat cgtaatcgtc
    20281 tctgtacctg cccccttaac tttggcccct attttgttta ggaaaatagc caagtcaaca
    20341 atttcaggtt ctcgcgctgc attttccaat accgttgtac cctgcgccag agttgcagcc
    20401 atcattaaat tttgcgttgc cccaacactt gggaaatcaa gatagatatg agctccctgc
    20461 aagttctctg cacttgcttc aatataacca gctcgttgtt taattttagc ccccatagct
    20521 tgcaaacctt ttaagtgcaa atttattgga cggctaccaa ttgtacaacc tcctggcata
    20581 gaaaccttag cataaccatt gcgtgccaaa atcggaccaa gaataacaat agaggcgcgc
    20641 atttgactca catacttata aggagtttcg ctaccaagtt tacctgttgc gtcaatatca
    20701 atagtctttg tttcctcgtc aaatttcaca cgaactccca gaccacgtac aacattattc
    20761 attgtataga catcagacaa aataggaacc tgagtcaatt tggtttgccc ttgacttgct
    20821 agaactgttg ctgccaataa aggcagaacg gcattcttcg ccccctcaat ctcaacctgt
    20881 cctttcaaac catttttact acctgtaaca ataattgtat ccatttctta tccttttctt
    20941 atcttaaatc acatatttgc aaaaaagcgt cccatcatca acaattctaa aaagaaagtc
    21001 gagaccaagt atcccagtgc tattgccaga aagagaatca gcaaattgat tttacgctga
    21061 ttgtccccag tcaccttcaa ccaccgtgac caatcaactg ttgtaaccaa gagatgatga
    21121 gccagataga taaacaatat atgactaaaa aactgtaaaa ttgaaattac catgtttcca
    21181 ttataccatg aaattaaaaa atggaactga accaagttca atcccatata gattaatgtt
    21241 tcccaacacg aatacgattt atcgcacgtt ggagggcgac tgttgcacgc atctccatat
    21301 caatattacg agcagtctgt gcttcttcca attcacgttc cgcgcgcaat ttggcacgtt
    21361 ccgcacgact aatatcaata tcccgttctc gctcagcgga atcagaaatg accgtaataa
    21421 tatccttgtt gacttcaatg attccaccat tgaccgcaat ccagtctaca tggttttcat
    21481 catcaacacg acgcactttc atttcaccga tttccaaaac tgcaatcagt tcttcatgtc
    21541 cagaatagac acccatctca ccatcaaccg ttttgaccaa aacaaatgct gcatgatggt
    21601 catatttgat accgtctggt gttacgattt gtacagtcat ttgtcccata agccacctct
    21661 agaatttcat tttagcagct ttagcgacca catcctcaat tgaaccaaca tttcggaagg
    21721 catcttctgg tagatggtcg tgtttgccgt ccaagatttc cttaaagcct ttcaccgttt
    21781 cagctactgg cacataagaa cctggcatac ctgtaaattg ctccgcaacg ttgaagtttt
    21841 gagagaggaa gaattggata cgacgagcgc gaccaaccaa ggtcttctct tcatctgaca
    21901 attcatccat accgagaatg gcaataatat cttgcaattc ttggtaacgt tgaaggacac
    21961 gttttacttc catagctact gcatagtgct cttcaccaac gatttgtgga gcaagtgcac
    22021 gtgatgaaga agccaatgga tccaccgcag ggtagatacc aagctgagtc aacttacgct
    22081 ccaagttagt tgtcgagtcc aaatgagcga aagctgtcgc tggagctggg tctgtatagt
    22141 catccgcagg tacgtaaata gcctgaatag atgtaacaga acccttcttg gttgaggtaa
    22201 tacgctcctg caattgcccc atctcagttg caagtgttgg ctgataacca acggctgatg
    22261 gcatacgacc caagagggca gacacttctg aaccagcctg cgtgaaacgg aagatattat
    22321 cgatgaatag aagaacatcc tgcccttcca catcacggaa gtattccgca atggtcaaac
    22381 cagtaagagc aacacgcata cgagctcctg gtggctcatt catctgacca aataccatgg
    22441 ccgttttttc aataacacct gattctttca tttcccagta aagatcattc ccttcacggg
    22501 tacgctcgcc aactccggta aatacagaga taccaccgtg ttcttgggca atattgtgaa
    22561 tcaattcttg gataaggacg gttttaccaa caccggcacc accgaagaga ccaaccttac
    22621 caccttttag ataaggtgct aggaggtcga taaccttaat ccctgtttcc aaaatttcgc
    22681 ttgaagtaga taattcgtca aaagccggcg ctttcttatg gataggctca cgttcaaaat
    22741 ctgccggaaa agactcttca aggtcaatgg tatctcccaa cacattgaag acacgaccca
    22801 gcgtttcttt accgactgga acagagatgg gacgacctgt atcgagaact tccatcccac
    22861 gtgtcaatcc atccgttgat tccatggcaa tggtccgtac aacgccatca ccaagttcca
    22921 aagccacttc aagcacgact ttttgtttag aatcatcatt tttatataca acgagtgcgt
    22981 tgttaatctc aggaagttta tcttctgctg caaacgcaac gtctacaact ggtccgacaa
    23041 cctgagtaat tttgcctgaa ctcattctat ttcctctatt tctattactc aagggccgag
    23101 gcaccagcta caatctcagt aatttcctga gtaatcgctg cctgacgagc cctattgtat
    23161 tggattgtca aatcgttaat gacattctta gcattatccg tcgctgtctg catggctgtc
    23221 atacctgctg cattttcagc agttttggca tccaaaatcg ctccataaat agttgattct
    23281 gcatattgag tcaagagttg ttccaaaata acctcacggt ttggttccaa ctcaaaggta
    23341 gctgtataac catctgcttc attgtggtcc aaatcttcta caggaagcat ctgttgtaca
    23401 cgcacctgac tggtcaaact attgacatgg tggttataac aaacatacaa ctcatcaaag
    23461 agttcattct tgtacatctc aacagactta gaaatgattt tttgaacctc ttcaaaactt
    23521 gggttgtctg ctaaaccacg caattcaaag acaggattga taccacgggc acggaagaaa
    23581 tctgctccca tactaccaat ggcaataatc tcatactcgt cttttgagtc atgatcctgt
    23641 tcaatcattc ccatcacagc cttcaaaata ctggaattat aagaaccttt tagtccactg
    23701 tcagatgtaa tgacaatata accagatttt tgaactggac gacgaatcag cattggattg
    23761 cttgtatctg aagccatcaa ttctccacgc aagaggtctg ttgtaatctg acgaacctta
    23821 ctagcgtaga tttgaaagga ttgcgctaat tgctctgatt tggctagttt agaagccgaa
    23881 accatctgca tggcaccggt gatttgactg gttttcttag tagatgcaat ttttgacttg
    23941 atttcattga gagaacctgc cattattcac tcctttactt aaagacagac tggtctttga
    24001 aagcttggat agcagcattc aattcatctg tatcaggaag atcttttgtt gtacgaataa
    24061 catccaaaag accatcgtag tgcaaatcaa aatatgcgta cagttcttct tcaaatgcca
    24121 aaatatcatc aattggcact gaatccaaga agccatttgt caaagcatac aaaatcaaaa
    24181 cctgtttttc aactggaagt ggcttatgca atggttgttt caacacttcc acagtccgac
    24241 gaccacggtt gagtttggcc tgagttgctg catccaaatc cgaaccaaat tgagtaaagg
    24301 cttctaattc acgataagaa gctaagtcga tacgaagggt acctgcaacc tttttcatgg
    24361 ccttaatctg tgcagagcca cctacacgtg atacagaaga acccgcatca atggctggac
    24421 ggataccaga gttgaataaa tcgtccttca agaagatttg tccatccgtg attgaaatca
    24481 cgttggtcgc gatgtaagct gaaatatctc ccgcttgtgt ttcgataaat ggtagagctg
    24541 taatggagcc accgcccaat tcatctgaaa cctttgctga acgctctagc aaacgactgt
    24601 gcaagtagaa tacatcccct ggataggctt cgcgacccgg aggacgacga agcaagagag
    24661 ataattcacg gtaagctacc gcttgttttg acaaatcatc gtaaacaatc aagacgtgtt
    24721 tgccttcgta catgaactct tctgccatcg caacaccagc ataaggagcc aagaaaagca
    24781 atggagacgg ttgtgaagca gatgcagtta ctacgattgt gtaatccaaa gcaccatatt
    24841 gacgaagcgt ttcaacctga gtacgaacag ttgactcttt ctgaccgata gccacataga
    24901 tacaaatcat gtcttggcct ttttggttga gaatagcatc aatagctaca gatgtcttcc
    24961 ctgtctgacg gtcaccgata atcaattcac gctgaccacg accaatcgga accaaagcgt
    25021 caatcgcttt aagaccagtt tgaagcggtt cattaaccga cttacgttgc ataacacctg
    25081 gtgctggata ttcaatcgga cgagtcttac tagtacgaat ttcaccaaga ccatccacag
    25141 gttgaccaag tgggttgatg acacgaccaa tcaaagccga accaactggt acttccataa
    25201 ttttaccagt acgacgaaca actgatcctt cacgaatatc ggtaaattgt ccgagaatga
    25261 taataccaac atcgttggtt tctaagtttt gtgccatacc aatggtgcca ttttcaaata
    25321 ccaaaagctc tccactcatg gcattgtcaa gaccttgcgc acgggcgata ccgtcaccga
    25381 tataggttac gacaccagtc tctgtgtagt caaagtcagg ctgaaagcct tcaatttgtt
    25441 gttttagtaa agcgctaatt tcttgtgcat taatcaaaag aacaccactt tctattttct
    25501 aaagtttttt tctaacgtcc ttcaactgtg tccgaacgct tgcatcgatt accttatggt
    25561 taaccgttac aataaaacca ccgaggagac tttgatcaag ttcttccgtg atattgcgta
    25621 cacgcaagga aaatcgttgt tctaccaagt gacggagacg ctctttttga gcttctgtca
    25681 acggatagac tgaaaccaca cgggcttcaa attcattttt aaatttactg atttgtaatt
    25741 ggacacgctc aagggtttca agaagcaagt ctgcatgacc atcacgaata acatcttcaa
    25801 tcaagtcatt gatttgccag aaactagatt gacgtaccgt ccttacaaag tcagcctttt
    25861 cttctctcga tactgtcgct gacagcaaga ttcgattcag cttactatca tgaatgatgg
    25921 aaatcaagtc agaaatctgg tcatacatat cccaaatatc tgcatgatca ctgacttttt
    25981 caacaaatga tagagcatat ttttgcacga tggcattttc tctagcgttc atctacttgt
    26041 ctcctagttt gtccaaataa cggtcaatca agttactatg agcttgttgg tccaaatctt
    26101 ccaaaataat tttgcttgca agatcaactg ccaattcagc aacttgaaca cgcaagtttt
    26161 cttgtgcttc acgtttttca gcttcaattt ccaattgagc tttttgcttc aatccttgaa
    26221 tttcaacatc tgcttgctca agaatacgtt ttttctctag ttttgcgcgt tcaaccgcgt
    26281 cttgaataat cttttgactt tcaatacgtc cctgtaccaa ctcatcttca cgttgttgaa
    26341 caaggtttgc tgctgctgtt aatttttctt ctgcagcatc aatatcgttc gcaatcttat
    26401 tggcacgttc ttcaaaaata cctgtaattt tatcccatgc aaaaacacga ataagaataa
    26461 taagtactgc aaatgaggct gttaccaaga taaagtttcc aagaatggta ctagattgca
    26521 ttgtaatagt tgttgccata gttgtctttc cctttcctat tcactctcac tactgatttt
    26581 tttatttaag tacatagata ccaacatgat aaatacatat ccctgcaagc atgaaatgaa
    26641 tacagaaaat gctgtccaaa caacgttcag agcaaaggca attggatatg caagtgcact
    26701 ttgttgagat aattgtagaa gaagagagac aagcacttca ccagcataaa tgttaccgta
    26761 cagacgaaga gcaagcgaca caaggttggt cacttcttcc aaaatgttca ttggagccat
    26821 agcccagggt gtcacaaaag atttgaggta tgccttgaaa ccgcgacgac gaataccctc
    26881 aacatgacaa aatactgttg caattgttgc caaaccgaaa tcgtaagcca tattggccgt
    26941 tggagaagtc cacaaattat gtccttctgc tgtttccaat ttggtcatca aacctagatt
    27001 gttggcaacc aaaagaaaag taaagactgt aaagaagaac aaggaatacc gctttgcttc
    27061 cgcatcgcca agattccctt ttgtaaaatt gatggtcaat tcgtaaacgt actccaaaac
    27121 attctgtttt ccttttggct tgagctccat ccgacgacta gcccaaaaaa caagtaagaa
    27181 aataatggaa atggtaatga cagaaaccaa taccatggtc aggtcaaagg ttacgggacc
    27241 aagggtaagg gttggactta aatgttcttc caatggaggt tccccccttt tctatttctc
    27301 tattttctac tatttcaaga caaatgtcat agccaaggtt acgaagaaag ttccctcgat
    27361 aaaggcaaca cccaaaaata caccagccat caatttactt tgcatttctg gttgacgtgc
    27421 agtcgaacta aggtatgaag ccaccaacag tccctcaccg atacttacac caagacaggc
    27481 caatccaagg gccaatgcgc ctaaactcat aacgaattct ccttttaaaa tttaatacca
    27541 tgtaaggata ctcctaaaac atctatttgt caagaaaata ccgccattgt aagcgtttaa
    27601 ttcgtttttg taaaataaaa agaaaagagt atttcatctt ttcttcctat ttaatagctt
    27661 catcaatatg gtctgtttct tcaatgaatc gtttggccat atcttctcga ttcatctgat
    27721 cagtatcaag agtgattgca tggtcagaaa ccagatctgt atctgcgaaa aactctatat
    27781 ggtttgatag gttgtacaga cgaacagggg cttccccacc aaactctcca tctagattga
    27841 gcagtagacg gttatctggg ttcagatttt cgatggatag gctcttggtt tttatgtatt
    27901 caactaaatc actctcaatg tgtttgccac cgtctaagac ttgacgaatc agatgcagaa
    27961 tttcaaatag attccctgtt ttaaccatca ataaggtaaa attaccatca tctaacttag
    28021 catcgggaac aatctgttca aaacctccaa ttgaattggt taaggcgaca aaaatcattg
    28081 aaaccgagcc ttcaaatact ccctcgtcat gctctacacg aacaggtgta aattgtactt
    28141 gtggcaggag ttctgctcct tttactacat aggccaaata gccaaacatg gtcttaagtt
    28201 gactgggaac actgtaggtc aactccgtaa gtgtacctgc cgcagcaata ttgataaaat
    28261 aatgttcttg atgaaaacca tttttttgat ttttggctaa accaatatcc attaaaatgg
    28321 tctgctgctt accgataacc ttagctgctt ctacaggatt tcccctgggc acttttaagg
    28381 cacgcgcata gtcattggtg gtgccagtcg gtatgatggc catctgcgga cgtttttcaa
    28441 gcggagcaat tccattgacg acttcattga tcgttccatc tccaccagcc gcgataatca
    28501 agtcaaagcc tgcaagcgct gcacgcgtag cttcattctt tgcagagtct ttctcagcag
    28561 ttgtttggaa agctgaagtt tcataaccgt agccttccaa aatttccaat acttcggcca
    28621 catttttctt catgatttct tgacctgagg tcggattata tattagtcta gcacgttttc
    28681 tttcttccat cgttttacct ttacaagctc tctaaccaag cctcatcttt aatttcgatg
    28741 cctagctcct gtgctttggt caatttactg cctgcatctg ttccggcaac cacaagatta
    28801 gtttttttag aaacagatcc cgctacattt gcccctaaag cttctaattt ggctttggct
    28861 tcgtttcgtt tcattcgctc caatttacca gtcagtacaa ctgtcatacc cgatagcgca
    28921 gcattttcat ctactacttg tccgagatag gttaagttta ccttagcttc cttaagttcg
    28981 gctaaaagtt gttgcgcacc ctcactagca aaaaaagttg tcaaagactt agcaataacc
    29041 tgaccaagac cttctagcga agcaattgct tcttgatcag caaatgctaa agtttctaaa
    29101 tcaccaaatt tttctactaa aatcttactt gccttactac ctacatggcg aatacctagt
    29161 ccaaatagga ggcgttctgc tgagttgctc ttagaagttt gaatggcttg atggagcttg
    29221 tcagctgatt tttctttgaa accttccaag gtcaataggt catcaacagt taatttataa
    29281 atatcagcaa catcgtgtac caagcctgcc ccaaagagtt tttcaacgat tgaagaccct
    29341 agccctgcga tgttcatggc atcccgactt gcaaaatgtt ccaacttact catcagctgg
    29401 ctaggacaaa tagggttgat acagcggagg gcaacttcgt cctcatagtg ttgtaggtcg
    29461 ctctgacaag acggacaatg actaggaatg ggcatgattt cttgctcttt tcgatacttg
    29521 tccaccactt tcagaactgc tggaataata tctcccgcct tataaacaat gaccgtatcg
    29581 ccaatacgaa tatctttttc ggcgatataa tctacgttgt gcaaggtcgc tcgactgaca
    29641 gtcgttcctg ccagttgaac aggactaaga ttggcagttg gtgtcacaac acctgttcgg
    29701 ccaacagtcc aatcaaccga caatatttct gcttcttttt cctcagctgg aaacttgtaa
    29761 gctactgccc atcgtggcgc tttaactgta aatcctagtt cttcttgtat agctaaatca
    29821 tttaccttga tgacaacacc atctatctca tagggtaaat catctctacg ctctgctatt
    29881 ttttcaataa aatcccacgt gtcatcaatc gactccgcca agcaatattc gtggttggtc
    29941 acaaatccca gagcatccag tttctccagt acttgtgact ggctagtcac ttcagatgga
    30001 gtagcctctt ggtagaggaa ggtagcgaga ccacgctggg ccaccactcc cgtatcaagc
    30061 tggcggaggg tccctgctgc tgcattacgc ggattagcaa attcagcttc accagcctcc
    30121 tgacgctgtt tgttaacacg gtcaaaggaa gcttttggca tataacactc acctcggaca
    30181 gtgatgtccg cagcttcggg tagggtcaaa ggaatatcag ccactcgttt gagattttct
    30241 gtgatatttt caccgacact accgtcacca cgtgtagcac ccacgactaa attacccgct
    30301 tcataggtca gggaaataga tagaccatca atcttcaatt cacagatata ggttgcttga
    30361 ggaaattcct tgcgaactcg ctggtcaaaa gcttctaatt cttcacgaga aaaggcatcc
    30421 tgcaaactaa aaagaggata aacatgacta tatttttcaa atccgtctag cacctttccc
    30481 ccgacccgat gggtaggact atctggtaaa atcagttctg gatgagctgt ttctagctct
    30541 actaattcac gataaagcgt atcatattct gcatctgata cgctaggttg gtccaattga
    30601 taatactctt tagagtattg attgagcacg ctaactaatt ctgatattct tgttttcata
    30661 agaatattat atcatgattt gagggtgcga actatatttg cgcttcaata aaaacagatt
    30721 cctttagata atttttagta aaaaatgtat aatgtttata taaaattttt ggaggtgccc
    30781 tatggctgta acctataaac gacaagatga tttagaaaag atgctggaag aatttgcttc
    30841 atttgaaaaa ttggaagaaa ttgagtatcc agatccaaaa tcaaaagaaa attccgggaa
    30901 aaacaacttg aatcaggata aaaaataata tgtttggtct tgatcaatta cctacctccg
    30961 tcctacaggc gggtgctatt ttcctgtcta tcatgattga agccttgcct tttgtcttga
    31021 ttggagctat tatttcaggt tttatcgatg tgtttataac accagataag gttcgtaaac
    31081 tcttacctaa aaacaagttt cttgccattc tatttggaac ctttatcggc ttcatttttc
    31141 cgtcttgcga atgtggtatt gtccctattg tcaatcggct attagagaaa aaagtaccta
    31201 gttacactgc tgttcccttc ttagtaacgg cgccggtcat taatcctatc gtcctttttg
    31261 caacctttac cgcttttggc aattccctac ttttcgccct ctatagagcc ctaggagcca
    31321 tcattgtatc cctagtattg ggaatcattc ttggtttttt cgtaaaagga ccaattctta
    31381 aaggaagcaa gtttgaccat caccagcatg attattcaga taaaaccagc tggcaaaaag
    31441 ttggcttcgc acttattcat gccattgatg aattttttga tacgggacgt tacctagtct
    31501 ttggttgtct atttgccagt ctcgtacaag tctatattcc aaccgctgtc ttgactacca
    31561 tcggtcacag tccattaaca gctattttac tgatgatgtt gctggccttt cttctttccc
    31621 tatgttctga agccgatgcc tttattggtg catccctact atccagcttc ggattcgccc
    31681 cagtaatggc tttcttagtt atcggaccca tggtggatgt taaaaacttg ctcatgatga
    31741 aacattattt caagggaaaa tttatagccg gttttatcat tactatcact ctcgttattt
    31801 tgggatatag tctgatactt ggaggtgtag tatgattcgt tttttaattc ttgcaggcta
    31861 ttttgagatg actatgtatc tttatgtctc tggaaaatta gaccaataca ttaatctgca
    31921 ctatagctat ctagcctatc tatccatgat tttatctttc atacttgcca tcgtacaatt
    31981 gtatgtctgg gtaaaagaaa ttcgagtaca tagccattta aaaagcaaaa ttgccaaact
    32041 atcaagcatt ggactattgc tcattcctct tgctatagcc tggctctttc ctacggtaag
    32101 cctggattca acaactgttg ctgccaaggg ctatcatttc ccactagcag cagaaaacga
    32161 tactaataca caaaaccaag aagggacgac tgttcagtat ctcaagcctg atacttctat
    32221 ttattttaca aaatctgcct atcagagcca gatgcgtgaa attgctaata gatacttggc
    32281 tgaagaaacc attgtcgtta ctaatgaaaa ctatatggaa gtgatggaag caatctacga
    32341 ctatcctacg gaattttcag gaaaaacgat tgaaatgatt ggatttgtct acaatgaccc
    32401 tgacaatagc cagcaatttt tccttttccg ctttggtatt attcactgca ttgctgattc
    32461 tggggtatat ggcttactct ctacgggtgc taccacagaa tttcctaaca atacttgggt
    32521 aaaagccaga ggaacagtca aggtttccta ccacacatcg ttgaaacaaa atctcccaac
    32581 acttgaatta caatcgatgg ttcagattga acagccagat tccccatacg tctaccgtgt
    32641 tttctaagaa ttccaactca aaaagtgaat tttctgacaa tttgtggtat actattaatg
    32701 atattcaaca gataggaatg aaattatgtc atcacatgta ttgtttacaa tatttggtgc
    32761 tagtggcgac cttgccaaac gcaaacttta cccatcttta tttcgcctct ataaggctgg
    32821 acacatcaag gaaaactttg cagtaattgg tacagctcgc agaccctgga ccaaggattt
    32881 ttttgagcaa accgtcattg aatcactagg tgatctgcct gacacaccgc gtcaagctca
    32941 tgaatttgct agtcatttct actaccaaag ccatgatgtc aatgacacgg aacattatgt
    33001 agctcttcgt aaattgcagg acaacttgtg tgaaaaatac aatacccagc acaacaaggt
    33061 cttcttcctc tctatggcac ctgaattttt cggaaccatt gccaaacacc tcaaatctga
    33121 gcaaatcgtt gatggacaag gttttgaacg cttgattatt gaaaaacctt tcggaactag
    33181 ccttgctaca gctactaaac tcaatgatga attggcagca gcctttaatg aagaccaaat
    33241 ctaccgtatc gaccattacc taggcaagga aatggtacaa aatatctttg cggttcgctt
    33301 tgccaatatc atttttgagc atatttggaa ccgcgattac atcgatagcg tccaaattac
    33361 atttgctgaa gcgattggtg ttgaggatcg tgggggctac tacgatcatt ctggcgcctt
    33421 aaaagacatg gtgcaaaacc atgcccttca agttctttcc cttctagcaa tggataagcc
    33481 agccagcttc caggaggaag atgtccgcgc tgaaaagatc aaggtcttcc aacaccttcg
    33541 tcagccttct gatgaggacc tcaaacgcaa cttcatccgt ggtcaatatg cagcaggttc
    33601 catcgacgga aaagactacg ttagctactt ggatgaacca aatattgccg aagcttctca
    33661 gacagaaacc tttgcaggtg gtgtcttctt cgttgatact gaccgtttcc gtgatgtacc
    33721 tttcttcttc cgtacaggta aacgtctgac agaaaagggc acgcgcgtga ccatcacctt
    33781 caagcatgcg gaagatattt ttggtcagcc ttctgaagcc aatgtattga ccatatttat
    33841 ccaaccaact gaaggcttca tgctctctat caacggtaag gaagttggtt cccactttgc
    33901 tcttactcct gccaaactca acttccgcca caatgcaact gctcttggaa attcacctga
    33961 agcctacgaa aaactatttt tcgatgtcct aaatggcgac tctaccaact ttagccattg
    34021 ggaagaagtg aaagcaagct ggagcttgat tgaccgcatt gttgatttgt gggcaagcaa
    34081 ccaagtccca ctccacacct atccagctgg aactatggga cctcaagcag cctttgattt
    34141 gctagaaagc tacggtcaca agtgggtttg gacacctgat gtctggtatc gtgaacgtgg
    34201 tttgttgaaa taagatataa aaactggagc a
//
LOCUS       DATUCG010000008        33476 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.008, whole genome shotgun sequence.
ACCESSION   DATUCG010000008 DATUCG010000000
VERSION     DATUCG010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33476
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.008"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            157..1887
                     /locus_tag="VCA20_000320"
     CDS             157..1887
                     /locus_tag="VCA20_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834190.1"
                     /translation="MLKKLLKKHPDIVAVTVFFLFSLTLLLPQILTKAVVTGDDIVFH
                     YNRFYDTAMQIKSGDFSYIMSLHGYQQSGRIVNALYGPYLAYLQGALLLLAGTWYRYQ
                     LLSNLLLGVLSATSLYFLMREVKVRYSLSVGLSMFFVTTYSVQYWWVAQGFSSWGVAL
                     FPLCLIPAVRFLKTGKVPIFLMAGAVGLMLQIHMLSALFLILAYAILFSIGWLKSQDK
                     WNVMKDVLFSVGVFLILTVNIWLPLLYVNATNELAAPFINKKFIQSTVTWSKAYFLYF
                     PYSLPIIFATSLYFMLKKWKKQSVILRGLTLTFLVFFIFSTNLFPWQLFAGKGIGLVD
                     LIQFPFRFFLYANALLLTIVGLQLTDYEVRKKLFLGLTILSTIIGVGFTWDQYNQAVN
                     DQYNKAEFLQDMLHTTLYGTTEELRASLHSKNLGEFVQLAQKSTPDYVPVLSEKEENV
                     RAVNEAKSRYDRYRDQMILPNQQFNKQVFGDSLKISWSANQSEEIQIPIVIYRDSELT
                     LNGKRLGKMDYYLSTIGVPTVTSQKGKNTLILSYKQQWWLLPVIFISLTGWILWSIYY
                     TFIYRKSNIS"
     gene            1900..4344
                     /locus_tag="VCA20_000321"
     CDS             1900..4344
                     /locus_tag="VCA20_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEP1834191.1"
                     /translation="MKQIKLKATKQFILVMVMTVLLAMGRALLLSDVSFPLWFIPLPL
                     IAYLIFTFYVLVLLDKYEKFIKTKTFAKYVGYFLGFLYLINLVYRLNAKKYQPWNIFR
                     NNLFQFELLLMLALPILLAFLWRKKTGIREKLSQWSANHLVPDGYLLLTSLLSLSPLA
                     ISYWKDSHYESLVEKGSYIEFFSQTPLFAPIHFIGTYIFLRYLHKAFVEFKANRTNVH
                     SMLFISLALASLSHIGYQASMAGGTGSYFTRHLFPGAIVLQIACLFFLNVIISLVINR
                     QILSVAVIASLNVILVTANFLKFRYRSEPMTPNDFKWIGNMGMILSFISLRVVLVSLV
                     FIVLLVFIYRRIHKNYFQGRIVASIWKRLAGISVIVSLILGMGWAIRNEKDHKIAGWI
                     PILSQVNNWRNVDWKGYAFVARYRTLSFLWLQQLSKTSMEMPENYSEKTMKAIVKKYT
                     ALAEEINAERTGQLTDQTVIYILSESLADPRRIPGVTLSQNVLPNIEYIMSQTTSGLM
                     KSDHYGGGTANIEFQVYSGLPFYNYSSSISSVYLDVAPNMKKLPSISDLYPADNRIAI
                     HPYYDTSYNRNSIYKQLGIEQFYTLNSAKYPLTVTAEDYQGNFVSDKKTYDLILDQVR
                     SADTSFVSAITMQNHVQWNSLEPAGITASGEGFTAEENENLTSYVRLLSFTDQATRDF
                     LDQLKTLDKKVTVVFYGDHLPGLYPESAFVTDPSAQYKTDYFVWSNFDTAKYNYELVN
                     SSDMNALMLETTNNKVSPYYALLTEVLHKDRVGQAERDAKVAEELKLVQYDLSTGKGY
                     LLKYKDFFKVATETTE"
     gene            4485..6062
                     /locus_tag="VCA20_000322"
     CDS             4485..6062
                     /locus_tag="VCA20_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1834192.1"
                     /translation="MKKRNFWLTLSCVFAVALLNVGFLYQYCYIIDAIAAGDRQAFVS
                     YFVQMGLTVLVMLVFEYIRQIANVSYLNQVGFQLQATFIGKIFDLPFRQFVEQGAGAY
                     ISQLNNDLETVKEDYYDAFFTIFQGACTFGIASLALLSLDGLTAIFLILISFLPVVIP
                     YLFKKRRRLNQEAISDSQQVYNTRVSDVFLSYLQVKNSRQRQQVLDGLNDRYQAVNDK
                     VNQANRTTVTMRLLVGFVFYLTTLSIIFIGGFQVLSGALTIGGLTAILTISEQLVDPI
                     NSIAAAFLDRHAVKKLKQEFELSTLGQETKGQELASAFQSIQLVKASYVIGEKQVFED
                     LTVAFERGKKYLILGESGSGKSSLALILTKNSQLQAGDIYFDQISLSDLSYQAIQDKI
                     AYLPQEGALFHDTVLYNLTMGREVPEDRLMFLIKTMNLDSRFSSYASLSEEISDDSGL
                     SGGQKQRLLLIRALLQDKDILLLDESLSALDQETYAVIENYLTSLADKTLIHISHRVS
                     DEVLSRYDGVVRIGENN"
     gene            complement(6595..6676)
                     /locus_tag="VCA20_000323"
     tRNA            complement(6595..6676)
                     /locus_tag="VCA20_000323"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6641..6643),aa:Leu,seq:cag)
     gene            6806..7483
                     /locus_tag="VCA20_000324"
     CDS             6806..7483
                     /locus_tag="VCA20_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEP1834193.1"
                     /translation="MKKIIKQYQEKGSVVIGYARVSSRDNRQELGLEVQKEALHFCDR
                     LYMEKESGGHQDRPQLQKAIRLAKACAESGIETSFVVYKLDRLTRKMLHLSAIISDLT
                     SHHIRLQSVHEQIETDSLTGRFFCLMLGYVAEWELQAISDRTKDGLRKARERGVKLGN
                     KGISKSKEKQVIQSYVAEETTIRDMVNQLNISTATIYQILKRNNIPFRRQKAHNSLTE
                     TGQNDRL"
     gene            7702..8745
                     /locus_tag="VCA20_000325"
     CDS             7702..8745
                     /locus_tag="VCA20_000325"
                     /inference="COORDINATES: protein motif:HMM:NF012948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEP1834194.1"
                     /translation="MKKIVTKKIGITLACTTLLGGVVAPVAVPAFQNIVHAEETNQQQ
                     PISVAVIAISETDGPLSHHYITINPGETKTISAPEVEGWKLDQYTNPTYELTYDQALK
                     TLNSAGFVWVQFWMIKDAAPTTKDPVIFTVAHRVAGGDDLLLDNAVVNPGESITLTPI
                     TVDGYVARADNKYTYTYDEVATGSSSLFVAEILYDKIAVEQPVEQPTEQPTEQPTEQP
                     VETPADHPVEQPADQPAEVPTEQAVEQPVEQPAEQPVDTPTDKPVEQLKEASSLTDQA
                     AGSVQTGKATDKKKIDNSTPSTPAVKPEEVSQPVAKTLPKTGDSSSMLLVLGGVLSGL
                     SGLGLAATSRKRD"
     gene            8904..9779
                     /gene="rsgA"
                     /locus_tag="VCA20_000326"
     CDS             8904..9779
                     /gene="rsgA"
                     /locus_tag="VCA20_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEP1834195.1"
                     /translation="MQGRIIKALAGFYYVEADGQIYQTRARGNFRKKGQTPYVGDFVD
                     FSAEENSEGYILKIHERKNSLVRPPIVNIDQAVVIMSAKEPDFNANLLDRFLVLLEQK
                     DMDPIIYISKMDLVEERAEMDAFKAIYEKIGYPFVYSLEELTPLLQDKVTVFMGQTGV
                     GKSTLLNRIAPELALETGAISDSLGRGRHTTRAVSFYNVFGGKIADTPGFSSLDYEVK
                     EAEALTDCFPEIAEASQNCKFRTCTHTHEPECAVKEAVASSAISQSRFDNYLQFLSEI
                     QNQRETYTKVSKKFK"
     gene            9794..10453
                     /locus_tag="VCA20_000327"
     CDS             9794..10453
                     /locus_tag="VCA20_000327"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEP1834196.1"
                     /translation="MSHYKIAPSILAADYANFEKELKRLEKTGVEYVHIDVMDGHFVP
                     NISFGADVVAAMRPHSKLVFDCHLMVSNPENHIETFARAGADILTIHAEATVHLHGTL
                     QKIRAAGMKVGVVINPGTPLVTIEPVLNLVDQVLLMTVNPGFGGQAYIPEVAEKIADL
                     VKLREAKGLNFDIEVDGGIDDKTIHSAKNAGANVFVAGSYLFKGDLDANVAKLRAALH
                     D"
     gene            10446..11078
                     /locus_tag="VCA20_000328"
     CDS             10446..11078
                     /locus_tag="VCA20_000328"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEP1834197.1"
                     /translation="MIKIAVIAGGSFDCLPEPADLYVGVDAGSLRLLDHSLPLDWAIG
                     DFDSVTPEELGRITDLAERFLQAPAEKDDTDLELALKEIFKAYPQDQVRIYGALGGRM
                     DHMMANLFLAAEPDLAPYMEQIELVDSQNIVRFRSAGQHRLAPIAGMKYISFMPSDQS
                     RLTIRHAKYPLDASNYFFKKCYSSNEFIDRDIDIQLDKGYVVLIYSKDKD"
     gene            11079..12317
                     /gene="rmuC"
                     /locus_tag="VCA20_000329"
     CDS             11079..12317
                     /gene="rmuC"
                     /locus_tag="VCA20_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEP1834198.1"
                     /translation="MDIVLLILLILVLLALVFLYGKWQSLALLLQDQAEDTADNLSDQ
                     LSYQLENATLKQQQAIHQEVERLRTELYQQLTDIRQELNKSHLETRDATDRRLQAIQE
                     SNEKRLEEMRQTVEEKLEKTLQTRLQASFETVSKQLESVNRGLGEMQTVARDVGSLNK
                     VLSGTKTRGIMGELQLGQIIEDILTPSQYEREFATVSGSSERVEYAVKLPGRTEGDYI
                     YLPIDSKFPLADYYRLEDAYESGDKGQIDLHRKNLLAAIKRFAKDIQSKYLNPPETTN
                     FGVLFLPTEGLYSEVVRNPIFFDELRRQENIVVAGPTTLSALLNSLSVGFKTLNIQRS
                     ADDISKVLGNVKLEFGKFSDLLVKAQKQLNQASSNIDKLLTTRTNAIERSLRTIDLYE
                     DDQTKGLLGLSPLDEEDNEN"
     gene            12307..13248
                     /locus_tag="VCA20_000330"
     CDS             12307..13248
                     /locus_tag="VCA20_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HEP1834199.1"
                     /translation="MKINQMKKDEFFEGFYLIKSAEVRQTRAGKDYLALTFQDDTGEI
                     EGKIWDAQPGKTKDFTAGTVVHMQGRREVYNNTPQVNQLVLRLPKAGEPNDPAEFKEK
                     PPVNVKDTKDYLSQMIFRIENATWQRIVRALYSKYDKEFYSYPAAKTNHHAFYSGLSF
                     HTATMVRLADKIGDIYPQLNKSLLFAGIMLHDLAKVIELTGPDNTGYTVRGNLIGHIS
                     LIDEEITKVLMELGIDDSLEEVTVLRHVILSHHGLLEYGSPVRPQIMEAEILHMIDNI
                     DAEMMMMLSALDKVGPGEMTNRIFAMDNRAFYKPNLD"
     gene            13248..14126
                     /locus_tag="VCA20_000331"
     CDS             13248..14126
                     /locus_tag="VCA20_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEP1834200.1"
                     /translation="MAVRIVSRQPLTKGWSTDQKYKVQLEDGRFGLLRIAERPAYEAK
                     RLEFQLVENLFGLGLPVAEPLSFWADDLSVYTLYEWVEGQDMNEVASSLSDSVLYELG
                     CQSGKFLRTLHALPIDQSQRDWNSFYQVKIDNKLAAYQAASHSYPNGQAMIDFVQANR
                     RLLEGRSIAYHHGDFHTGNFLLGADGKLKILDFDRYDIGDPWEEFNRLIFTADLSPAF
                     AHGQVDAYFDGAIPEEFWKLMALYVTVNSLGALSWAEQVDSEQIPLMKLQAATISEWY
                     ADFTCWIPTWYGQVYG"
     gene            14215..15027
                     /gene="purR"
                     /locus_tag="VCA20_000332"
     CDS             14215..15027
                     /gene="purR"
                     /locus_tag="VCA20_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEP1834201.1"
                     /translation="MKLRRSERMVVISNYLINHPYELTSLNTFAEKYESAKSSISEDI
                     AIIKKAFEESSIGQIETITGASGGAVFTPSISKEESIEIAQALRDQMAESNRILPGGY
                     IYSSDLLSTPHILKNVGRIIANAFKEEKIDAVMTVATKGVPLANAVANVLNVPFVIVR
                     RDLKITEGSTVSVNYVSGSSDRIEKMFLSKRSLKAGSRVLIVDDFLKNGGTINGMISL
                     LSEFDSTLVGVAVFAENQKGDRSVANYKSLLAVTDINVKENRVDVELGNIFD"
     gene            15122..15991
                     /locus_tag="VCA20_000333"
     CDS             15122..15991
                     /locus_tag="VCA20_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP1834202.1"
                     /translation="MKVLLATRLKNRRKELGWSQKELAEGVCDQGQISRIEKGTYMPG
                     ADLLHALAKKLQVRMDYFFDEEESEIVSDLKQFRKLAKNCLMQRDYEALGFLYEREKG
                     SLSHLTFPDQVYLEWVEAVLAEYLEARREDAIQKLRELLERVGENRRGYLYLYNSLLI
                     LLIRDEKNAEAEQFYSEVIEKIDKNKLDLLVDIDAYFSIQSNYIRYLWLTNQLEKGIE
                     LVTACIEEFKSIYPIHFLADFYCDLGNVTEDFADKQLVKERYELSRLLYELAGIDAIA
                     LKIEKYLKETYTD"
     gene            16263..18481
                     /locus_tag="VCA20_000334"
                     /pseudo
     CDS             16263..18481
                     /locus_tag="VCA20_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938433.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
     gene            18474..18941
                     /locus_tag="VCA20_000335"
     CDS             18474..18941
                     /locus_tag="VCA20_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEP1834203.1"
                     /translation="MSKLEVLENKKLVLKKVICKKLSSLQFEDLEQEIDKFYQHLQLL
                     KVQMFGPLIVKNSGTILHEDGTITVDYDLYVQAHDFRQYDKFYTVYEEIVCPHCVYVR
                     FEDSPEYLQYAYSKLDLHFYENDLETDGIIYTVYVNTNNETMTVDIFRPIISL"
     gene            18938..20341
                     /locus_tag="VCA20_000336"
     CDS             18938..20341
                     /locus_tag="VCA20_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEP1834204.1"
                     /translation="MKLYNKSELRYSRIFFDKRPPAYAFILIFSTLATLIIAFAVSAY
                     LPKNYIVKARGTSVITGTEYVSALSNGKIVTLHKKEGDSVKAGEVILSLSSGQEGLQT
                     ESLTKQLDKLKAKESLFQKYEQSLNEKVNYMANSGEEQEYYGKVEYYLSQLSSENYND
                     GSQYAKLQEEYTKLNKLVAEKSQLETDLSSYQSQLANLEAELATLSVQETAETNSDSE
                     TSSSSVDTSSNSIQLEQLNAKISEVKTKIETTTSSIQGKGTEIESLQSSIREMERSYN
                     DPTSQAYNTYAQLISELGTARAANNKSITELEANLGIATGQDRGLTVSASSDGTLHYV
                     TPLKQGMSVQQNQTVAEIAGSDREAYVEAFVLATDISRVTVGAEVDIAITGVNSQKFG
                     TLKGAVTQIDSGTITQETQNGNVSFYKVVVALEELTLKKDEEIVVLQKDMPVEARIVY
                     DNETYLDWILELLSFKQ"
     gene            20482..20790
                     /locus_tag="VCA20_000337"
     CDS             20482..20790
                     /locus_tag="VCA20_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834205.1"
                     /translation="MELVMPNNYVVLEEEEMMYLDGGEIATATVLGIISAAVAAGGAA
                     YGAGLAAGTRVYYAGLRNSQYQKIKWQVRAAALVVGNVWGGIFMTGFENAFYAKVTGK
                     "
     gene            21247..21564
                     /locus_tag="VCA20_000338"
     CDS             21247..21564
                     /locus_tag="VCA20_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834206.1"
                     /translation="MELVLPNNYVALEQEEMMYLDGGDWNTFKNNIKGLAASSAGFRD
                     AARQVGLWGAIWSARVYSYSYVVSVLAPILGSVTAANFVLGAIGVATVGAVSYALWNY
                     KYY"
     gene            21570..22469
                     /locus_tag="VCA20_000339"
     CDS             21570..22469
                     /locus_tag="VCA20_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834207.1"
                     /translation="MLIFLRFLLMVSLYGLWLEIHLLFVSLAFNLEPLTGYLLITCLT
                     FLTIFLVKILLDFLYLIKSLYQKECIFTVFPFILIKRQKMNIRLSPYFSFHRKYLSNS
                     SLKYSLLSLFLCVIALMTTIILKKPILSIVFTAILFWTIVEITHIIFNPMEIIFTDYK
                     WKKGDLFDSVLSKLLKNQIQTSEISYSNLMSILLYDAINQCTFLTDGDFFEDIFSKIM
                     DSEDIVLCTGLVELLMYEGLIGKNNNYLEKCEKIRNHLIMIERLDFFCYTSWLKYNFD
                     LCVNREYGKIEVRKILISNKKII"
     gene            complement(22632..23507)
                     /locus_tag="VCA20_000340"
     CDS             complement(22632..23507)
                     /locus_tag="VCA20_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEP1834208.1"
                     /translation="MKIDIRIPQAFPQLTVKEVLEDYFLIPRKIRHFLRTKKHVRVNG
                     ELINWQSPIAAGDLLQLTFDQEDYPEKSIPMGQGNLVEELYQDEHLIIVNKPEGMKTH
                     GNEPTEIALLNHVSAYVGQTCYVVHRLDMETSGAILFAKNPFILPILNRLLEDKVIYR
                     DYLALCQGQLKETDWTITDKIGRDRHDRRKRMVDNRKGQAALTQVRLLKPLGKNSLVS
                     CRLQTGRTHQIRVHLAHHGHALIGDPLYSRVAAPRLMLHAQKLSLTHPLTLEEISVEA
                     RSESFEKARDRLKIT"
     gene            complement(23509..24477)
                     /locus_tag="VCA20_000341"
     CDS             complement(23509..24477)
                     /locus_tag="VCA20_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEP1834209.1"
                     /translation="MTQVYDITIIGGGPVGLFAAFYAHLRQAKVKIIDSLPQLGGQPA
                     ILYPEKAILDIPAFPSLTGQELTDNLLAQLAPFDTTICLNETLTAIEPGETITLTTNK
                     GCHQTKTLIIAMGGGAFKPRPLEIDGADSFDNVHYHVSNIQQYADKDIVVLGGGDSAV
                     DWSLAFEKIAKTTQIVHRRDNFRALEHSVEELKQSSVTIHTPFVPKGLAGENGRASAI
                     DFDKVKSEDKLTLSFDHLFVNYGFKSSVGTLKEWGLELNRHRIVVNSKQETSVPGIYA
                     IGDCCFYEGKIDLIATGLGEAPIAVNNAMNYLNPNEKVQPKHSTSL"
     gene            24630..26843
                     /locus_tag="VCA20_000342"
     CDS             24630..26843
                     /locus_tag="VCA20_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEP1834210.1"
                     /translation="MDDLQHQPIEVPEDFQIDHEDRPSRRSRGNRGSVDKPKKKSRIP
                     EPIRKFWRRYQLTKILMILVGVLILTVGGYLFYLAKTANVGDLQQALKATTIIYDKDG
                     AEAGTLSGQKGTYVELDAVSDNLENAVIATEDRSFYKNSGINYQRTILAALTLGRSGG
                     GSTITQQLAKNAFLTQDQTISRKAREFFLALEINKKYSKQEILTMYLNNAYFGNGVWG
                     VEDASQKYFGISASDLTLEQAAVIAGMLKGPEIYNPYYSLENAVNRRDTVLQNMVNAG
                     YIDQATADAGFQVDLASQLSDTYTGKQSNYSYPSYFDAVIAEAIERYGLKESDIINNG
                     YRIYTEMDQNSQASMQVIFNDETMFPTSSFDGTHAQAASVALDPRTGGVRALVGRVNS
                     SEDAVFRTFNFATQAKRSPGSTIKPLVAYAPAVASGWSIDMALDNHTETYGDYTLHNY
                     DYSTSDTIPMYQALALSYNLPVAYIVNTLGIDKAFEYGQKFGLDMENVKKVLGVSIGS
                     GVETNPLQMAQAYATFANKGVMKDAHLITRIETASGKVLAEHRETSSKVIDRSVADKM
                     TAMMLGTYTNGTGVTADAANYYIAGKTGTTETSFDVNLVNDQWHIAYTPDLVISQWVG
                     FEQTDENHYIDSSSYWMAPSVFQTVANSILPYTAGTQFEVENAYAQNGIVEVAPDEAA
                     QTEPSLGQEQVQEISDRAKNLIEQTKQNLIDAQLPERAKTLWDNITSWFGELSNP"
     gene            26877..27029
                     /gene="rpmG"
                     /locus_tag="VCA20_000343"
     CDS             26877..27029
                     /gene="rpmG"
                     /locus_tag="VCA20_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEP1834211.1"
                     /translation="MALKKASLACTVCGSRNYSISLSSNPKPTRLEVNKFCKHCGQYT
                     VHKETR"
     gene            27039..27215
                     /gene="secE"
                     /locus_tag="VCA20_000344"
     CDS             27039..27215
                     /gene="secE"
                     /locus_tag="VCA20_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEP1834212.1"
                     /translation="MKFIKDIIRILKDTSWPTRKQSWTDFVSVVEYTAFFVAVVYLFD
                     LILSRGIMSLINLF"
     gene            27325..27864
                     /gene="nusG"
                     /locus_tag="VCA20_000345"
     CDS             27325..27864
                     /gene="nusG"
                     /locus_tag="VCA20_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEP1834213.1"
                     /translation="MYDSFDKGWFVLQTYSGYENKVKENLLQRAHTYNMLENILRVEI
                     PTQTVQVEKNGEVKEVEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKP
                     TPLLEEEIRQILVSMGQTVQEFDIDVKVGDTVRIIDGAFTDYTGKITEIDNNKVKMVI
                     SMFGNDTIAEVNLSQIAEL"
     gene            28007..33085
                     /locus_tag="VCA20_000346"
     CDS             28007..33085
                     /locus_tag="VCA20_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEP1834214.1"
                     /translation="MKQKWSQIENKQRFSIKKLSVGVASVSIGFFITGVPMVQADTSG
                     EGLESTVAVAMDMDSRQNSAVEKKEDGPLSDEPVKTEQVDEPVVEEGVVEEVVDTEAD
                     EEPVLSVDRAATDIKTTAGKTTEESKEKEDISGKEASAPQTIPQESQLEPEEVTTGRY
                     ILQFSEENRNLVLDKLKKIDDVKIVHEYKEVLTGASVEVGKESLSDVKAITELTSLEE
                     SRRIRPTLHTAKQLVGALKASSKYQTDGRGMVIAVIDSGLDIKHKDMRLDDGVIPKIK
                     DITPSTTGTYTLKVPHGYNYVSGNDNLYDDTHEPHGMHIAGTLAGNATDEEVASKKGV
                     DGIAPNAQLLVYKIFSNDPKNYKAETEDAAYAAIEDAIKHGADVISLSVGYYDSGLPG
                     NAYYTIAKRAAEKGIIITAAIGNAGASSSDTSFDLHTNNALGAVDTATTVGVAATPAV
                     IGVGSARNTHLVQREFMLNGQSFGYYPIGYTTLTEGKYEFVDAGNGRWEEVQGLDLAG
                     KVAIIKKDKFDLKDAVRNLKFKDVAGLVVINTDQGWNKDYYRTHQLLVDDKTLLSYSS
                     IWGISLSGEDGRRLLEVANQSQGNTGLVLKPTIGMKKLIEVPTVSGFSSWGPTVNLEL
                     KPEIVAPGEDVYATLNDNRYGSMSGTSMASPIVAGASALLLPRIRQMTPPEGMTRMDL
                     LRIILMNTATPLIDVLDSSGHALENSPRQQGAGLLQIDRAFETDVILHHRLKGGVELK
                     EIGRETEFEVTLENLGNQQRSFAISAGKVLTSQDVPVDRIGRSGKIVKEIHAAEIKGS
                     SIHLSEQSIQLGPKERKTIRLRLHAGEAKDQFAEGYIYFKSLTEGQSDISIPYFGFVG
                     DWSKERIVDAPAWETSSKLKLTSVLSSYKHNKSGRYIELGREKIQDNQSPLNPDNIAI
                     QNQHSDSQTGNAFVRFALLRDITNYDLDIVKEATEDAPVLRRIDTGTMLSRVRYADYF
                     ESLSEYSKLRTPIELHRWDGKVYDASNDENIPVPEGQYFFRLRVKNKENGAYQYTYLP
                     VKIDNQKPEIVAINTNRLTSHRELVVTAKDNNKVWEVRANLNGENLLVEKVVDDAGQL
                     HYHLKEVELPLDAKNHLRVEVMDIAGNVIAVEKDLVAPVIQFKNLEDLMAIRSKKTVE
                     IKANVSAQVSDVQANLDAQAVNYSLENGQLSLQIPDQSDGRHSFELILKDKDGNPIYT
                     KTLKYLVDNEKPTIDLDIEKDEEDEEVIQIGKNGRFTLKGKVSDNVSLPKDIKLYYSN
                     LDIGKGELKIIDVKEDGSFEQDFFKSDFPRAIMLTAVDEKGNKLKDLRINTSPESLDE
                     EEETEVPITVNNWLIDPIRFNKESLGRELDSGLVDFKKQEDGTYLFTFEIEAETDQAH
                     SVRINGGEKRYFEDGKLTYPVTLIEEGNVVDISVYNEADELTYTKKYQMLVDTENPVL
                     QLENEVLPLERQVVDSEEDEDEENQYAGVLLADADGHLTLTGSAKDNGIYWSLKINED
                     FVARGGFWRQYGNNEKAFRYELHSLKDGDTVKLDLSDSFGNAVVKKYKVRLNDKEVSE
                     QVPEKDLHVEQSDKDQTPSIPIPKSEAHVPMPKEDNSLAPQTESTEIALLTGDTREDG
                     VEHLVRLTKHEEALGISDERIEVSVPHREFFERSRRGETGALVADTSGKLPQTGDSLG
                     SVFISALLGLFGGAMALGNLKRKE"
     gene            complement(33128..33198)
                     /locus_tag="VCA20_000347"
     tRNA            complement(33128..33198)
                     /locus_tag="VCA20_000347"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33164..33166),aa:Cys,seq:gca)
BASE COUNT         9913 a         6188 c         7652 g         9723 t
ORIGIN      
        1 tgcgtatggc gtgtcccgcc ttccttgttc gttgacagtt gtgagaaagc ttgctatact
       61 gaaatgtaac taacttttga aaaatattgt gtgcaaattg gcagaatatt tacaaaaaca
      121 agagaggggt tccctctttt tttatgaagg tatcttatgt taaaaaaatt actgaaaaag
      181 catccagata ttgttgcagt aactgtattt tttctttttt cgctcaccct tctattgcca
      241 cagattttga caaaagcagt ggtgacagga gacgatattg tttttcatta taatcgtttt
      301 tatgatacag ctatgcaaat taagtcagga gatttttctt atataatgag cttgcatggc
      361 tatcagcaat ctggaaggat tgtcaatgct ctgtatggtc catatctagc ctatttgcaa
      421 ggagctttgc tgttgttggc cggaacttgg tatcgatatc aactactgtc taatcttctt
      481 ttaggagttt tgagtgccac ttcgctttat tttttaatgc gagaagtgaa agtacgctat
      541 tctctatctg ttgggttatc catgtttttc gtgactacct actctgttca atactggtgg
      601 gtagctcaag gattttcaag ttggggagtg gccttatttc cgctttgctt gattccagca
      661 gttcgattcc taaaaactgg taaagttcct atttttctga tggcaggagc tgtagggtta
      721 atgttgcaga tacacatgct gtcggctctg tttctcatct tagcctatgc aattcttttt
      781 tcaattggtt ggttaaaatc gcaagataaa tggaacgtaa tgaaagatgt actgttttcg
      841 gttggagttt ttctgatttt aactgtaaat atctggttac ccttattata tgtaaatgca
      901 acaaatgaat tagcagcacc atttataaac aaaaagttta ttcagtcaac agttacctgg
      961 tctaaggctt atttcttata ctttccatac tctctaccaa tcatttttgc aacctctctt
     1021 tattttatgc tgaaaaaatg gaagaagcaa tcggtgatat tgaggggtct gacattaact
     1081 tttctggtat tttttatttt ttcaacaaat ctttttccgt ggcaactatt tgctggtaaa
     1141 ggtattggat tagtggattt aatccaattt ccctttagat tctttttgta tgctaatgct
     1201 ttacttctga cgattgttgg tcttcaatta acagattacg aagtacgtaa aaagttgttt
     1261 ttggggttga caatcttatc taccataatt ggtgttggtt ttacttggga tcaatataat
     1321 caagcagtca atgaccagta caacaaagca gaattcttac aagatatgct tcatactact
     1381 ctatacggaa caacggaaga attacgtgca tccttgcatt caaaaaattt aggtgagttt
     1441 gttcaactgg ctcagaaatc aactcccgac tatgtgccgg tgttatctga aaaagaagag
     1501 aatgttcgtg cggtaaatga ggctaaatct cgttatgatc gttaccgtga ccagatgatt
     1561 ttacctaacc agcaatttaa taagcaagtt tttggggata gcttaaaaat aagttggtcg
     1621 gcaaatcaat cagaggaaat ccaaataccg attgttattt accgagatag tgaattaaca
     1681 ttgaatggaa agaggttagg gaagatggat tattatcttt ccactattgg tgtgccaacg
     1741 gttaccagcc aaaaagggaa gaacaccctt atcctctcct ataagcaaca atggtggtta
     1801 ttgccagtta tctttatcag tttgactggc tggatacttt ggtcgattta ttataccttt
     1861 atctaccgaa aatcaaatat ttcctaagag agaaatgcta tgaaacaaat caaactcaag
     1921 gcaaccaagc aatttattct ggtcatggta atgaccgtct tgctggctat ggggcgagcc
     1981 cttttactga gtgatgtatc ttttccactc tggtttattc ccctgccctt gattgcctat
     2041 cttattttta cattttatgt attggtcttg cttgataaat atgagaaatt tataaagacc
     2101 aaaacctttg ccaagtatgt cggctatttt ctaggttttc tatatcttat caacctggtc
     2161 tatcggctca atgccaagaa gtatcaaccc tggaatattt tccggaataa tctctttcaa
     2221 tttgagcttc tattgatgtt ggctcttcct atattattgg catttttatg gcggaagaag
     2281 acgggaattc gagaaaaatt aagccagtgg tcagccaatc acttggtacc agatggctat
     2341 ttgctcttga ccagcttact ttctctcagt ccactagcta tatcttattg gaaagatagc
     2401 cattatgaga gtttggttga gaaaggttct tacatagagt ttttcagcca gacacccttg
     2461 tttgcaccta tccattttat agggacctac atttttcttc gctatcttca taaggcattt
     2521 gtagagttta aggccaatcg aaccaatgtc cacagcatgc tctttatcag tttggcactg
     2581 gcctcgctgt cgcatattgg ttatcaggct tctatggctg ggggaacggg ttcttatttc
     2641 acacgacatc tctttccagg ggctattgtc ttacagattg cctgcctctt tttcctcaat
     2701 gtcatcatta gcctagttat caaccgccaa atcttatctg ttgccgtgat tgctagccta
     2761 aacgtcattt tggttacggc caattttctc aaattccgct accgttcgga gccaatgaca
     2821 cccaatgact tcaaatggat tggcaatatg gggatgattc tcagcttcat cagtctacgt
     2881 gttgtcttgg tatctctcgt ctttatagtc ctccttgtgt ttatctaccg acgtattcat
     2941 aagaactact tccaaggaag gattgtcgct tctatctgga aacggctggc aggaatttca
     3001 gttatcgtat ccttgatttt aggaatgggc tgggctattc gcaatgaaaa agaccataag
     3061 attgctggct ggattcctat tctttctcag gttaataact ggcgtaacgt ggactggaag
     3121 ggatatgctt ttgtggctcg ttatcgcact ctttcattcc tctggttgca gcaactcagc
     3181 aagaccagta tggaaatgcc tgagaattac tctgagaaga ccatgaaggc cattgtgaag
     3241 aagtataccg ccttggctga ggaaatcaat gcagaacgga caggtcaatt gactgaccag
     3301 acagttatct atattcttag tgagagtttg gctgacccgc gtcggattcc aggtgtaacc
     3361 cttagtcaaa atgtccttcc aaatattgag tacatcatga gccagactac cagtggcttg
     3421 atgaagagtg accactatgg tggtggtacg gctaatatcg aatttcaggt ctatagtggt
     3481 ctgcccttct acaattacag ctcatccatt tcttcagtct atctggatgt ggcgccaaat
     3541 atgaagaagt tgccaagtat cagtgacctc tatccagcag ataatcgtat tgccatccat
     3601 ccttactacg ataccagtta caaccgaaat tctatctata agcagctggg gattgagcaa
     3661 ttctacactc tcaatagtgc caagtatcct cttactgtga cggctgagga ttatcagggg
     3721 aattttgtca gtgacaagaa gacctatgac ttgattttgg atcaggtacg ttctgccgat
     3781 accagctttg tgtcggctat taccatgcag aaccatgttc agtggaattc tctagagcct
     3841 gcaggtataa cagcatcggg tgagggcttc acggctgagg aaaatgaaaa cttgaccagc
     3901 tatgttcgcc tcctatcctt tacggatcag gcaacaaggg attttctaga ccagcttaag
     3961 accttggata agaaagtgac agttgttttt tacggtgacc acctaccagg tttgtatcca
     4021 gagtctgcct tcgtgacaga cccaagtgcg cagtacaaga cagattattt cgtgtggagc
     4081 aattttgata cagcgaaata taattatgag ctagttaact ctagcgacat gaatgctctc
     4141 atgttggaaa cgaccaataa caaggtcagt ccgtactatg ccctcttgac ggaagtgctc
     4201 cataaagacc gagtaggaca ggctgagcga gatgcgaaag ttgcagaaga actcaaactg
     4261 gtccaatatg acctatccac aggaaaaggc taccttttga aatacaaaga ttttttcaaa
     4321 gtagcgactg aaacgacaga ataaaaatag accaggtcag aatgttgact tggtttttct
     4381 ttatgtgaaa aaaacacaaa catgggaagt tgtggtataa taacggtgtt taatacagat
     4441 tttttattgc tttgcttaga aatctatcaa acagtggagg agaaatgaaa aagaggaatt
     4501 tttggctaac cttatcttgt gtttttgcag tagccctgct aaatgttggc tttctgtacc
     4561 aatattgcta tattattgat gctattgcag ctggagatag acaggccttt gtgtcttatt
     4621 ttgtgcagat ggggttaact gtccttgtca tgctggtttt tgaatacatt cgtcagattg
     4681 ccaatgtcag ctatctcaat caagtaggtt tccaattgca ggcgaccttt attgggaaaa
     4741 tttttgattt accttttagg cagtttgttg agcagggggc aggggcctat atttctcagc
     4801 tcaataatga tttggagacg gtgaaagaag attattacga cgctttcttt acaatttttc
     4861 aaggtgcttg tacctttggc attgccagtt tggcccttct tagcttggat ggtctgaccg
     4921 ctattttctt gattctgatc tcattcttgc cagttgtgat tccctatttg tttaaaaagc
     4981 ggagaaggct caatcaagaa gcgatttcgg acagtcagca ggtctataat acaagggttt
     5041 cagatgtctt tctgagttat ttacaagtga aaaatagccg ccaaagacag caagttttag
     5101 atgggctgaa tgatcgctat caagcagtca atgacaaggt caatcaggcc aatcgaacga
     5161 cagtaaccat gcggctctta gtcggtttcg tcttttatct gaccactctt tcgattatct
     5221 ttatcggtgg ttttcaggtt ttatctgggg ccttgaccat cggtggcttg acagccattt
     5281 tgaccatttc agagcagttg gtggatccca ttaatagcat tgcggcagcc tttttggaca
     5341 ggcatgccgt taagaaattg aagcaagagt ttgagctatc tactttgggt caagaaacga
     5401 aggggcagga actagcttca gcattccaga gtattcaact ggtcaaggct agttatgtca
     5461 tcggtgaaaa gcaggtattt gaagacctga ccgtagcgtt tgaacgtggc aagaagtatt
     5521 taattcttgg tgaaagtggt tctggcaaat cttccttggc tcttattttg actaagaata
     5581 gccaactgca agcaggggat atttactttg atcaaatctc tctttcagac ttatcctacc
     5641 aagccattca agacaagatt gcctatctgc cacaagaggg agcccttttc catgatacgg
     5701 ttctttacaa cttgaccatg ggacgggagg ttccagaaga taggctgatg tttctcatta
     5761 agaccatgaa ccttgatagt cgtttctcaa gttatgcttc tttgagcgag gaaattagtg
     5821 atgatagcgg actgtctggc ggacaaaagc aacgcctgct cttgatccgt gccttgctgc
     5881 aagataagga catcttgttg ctggatgaaa gtctgtctgc cttggatcag gaaacctatg
     5941 cagtgattga aaattacctt acctcactag ctgataagac gctcattcat atttcccacc
     6001 gtgtgtccga tgaggtcctt agccgttatg atggggtggt acggattggg gagaacaatt
     6061 aatactcttc gaaaataaaa atcaggcgtt gttgacttga tttgatgagc gaggctgggc
     6121 aaaaagcaca gcaccgcttc acaaaattag tgtcaacatc tcagcgcagt ggttgattgg
     6181 tagatttgtt cgtgttttat actccaaatc tggccattac gactgttgcg aacaaagttc
     6241 gccttatctc caacctcaaa ctgtcttcca gacagtttga gctgtgcggg ggtgggagtg
     6301 aaacagtctg ggaatagact gtttcagctc aacaactaga aataaagact tgttgacgaa
     6361 ctcttctatg aatggtcgag ttctttccca ctcccagcct gttcctttga aacaaaatag
     6421 taacagggtt ctatctgcgt aagtagaaac gaactacggt cgctctattt ccaacctcca
     6481 aaggttccca aaacctttgg agctagtctg tttttgattt tcattgagta tgagcgagaa
     6541 aatataaaaa taaccccaag tcatcaactc tgacttgggg tttctcgttt atcatgcgga
     6601 ggaagggatt tgaaccctca cacccgatgg gcacatgcgc ctgaagcatg cgtgtctgcc
     6661 gttccaccac ctccgcttgc tttttattat actatttttt tcttgacttg gctaggggaa
     6721 atggaatgat tatcaaacca tgatcacatg ttcatcagac cttttatacc aacccccgcc
     6781 acaatagtag aacaaggagg aaaatatgaa gaaaatcatc aagcaatacc aagaaaaagg
     6841 atctgttgtc attggctacg cccgtgtgag ttcgagggac aatcggcaag aactggggct
     6901 agaagttcaa aaagaagccc tacacttctg tgacagactc tacatggaaa aggaatcggg
     6961 aggtcatcaa gaccgcccac aacttcaaaa agctatcagg ctagccaaag cctgtgctga
     7021 aagcggtata gagaccagtt ttgtagtcta taagctagac cgcctaacca gaaagatgct
     7081 ccatctgtct gctatcatct cagacttgac cagccaccat atccgcctgc aatccgtcca
     7141 cgaacaaatc gaaacagact cgctgacagg tcgattcttc tgcctcatgc taggctatgt
     7201 ggcagaatgg gaactccaag ccatttctga ccgtacaaaa gacggtctac gtaaggccag
     7261 agaacgtggt gtgaaattgg gcaataaggg tatttcaaaa tcaaaagaaa aacaagtgat
     7321 tcagagctat gtggcagaag aaacaaccat ccgagacatg gtcaaccagt tgaatatttc
     7381 aacagcgacc atctatcaga tcttaaaacg aaataatatc ccatttcgtc gtcaaaaagc
     7441 acataattct ttgacagaaa caggacaaaa tgatagactg taggtaaata catttgtttt
     7501 agcagacggt cgttaggtaa aacaattaaa gttgtcaata gaaaatattc aaaatgctga
     7561 tttgacgggc ttttcaagcc ttatcaagtt ggcatttttt gatttttcga tgttttagtt
     7621 aacgaccctt aactaaaaca ggtacaaacc ctattgtagc aggggttttt ggataaaaat
     7681 gaaaaaggaa gttggaaaaa catgaaaaaa atagtcacaa agaaaatcgg cataaccctt
     7741 gcttgcacca cactccttgg aggggttgta gcacccgtag ctgttccagc ttttcaaaat
     7801 attgttcatg cagaagagac aaatcagcag cagccaattt cggtagcagt aattgcaatc
     7861 tctgaaactg acggtccatt gtctcatcat tacattacca tcaaccctgg ggagacaaag
     7921 acaatctccg caccagaagt tgagggatgg aagttggatc aatacactaa tccaacttac
     7981 gaacttacct atgatcaggc cttaaagacc ctaaactctg ccggctttgt atgggtgcag
     8041 ttctggatga ttaaagacgc tgctccaaca accaaagatc cagtaatttt tactgttgct
     8101 cacagggtgg ctggtggtga tgacctttta ctagacaatg cggtcgtaaa tccaggtgaa
     8161 tctatcacgc ttacaccaat cacagtagat ggatacgttg caagagctga caataagtat
     8221 acttatactt atgacgaggt tgctaccggg tctagctctc tgtttgttgc agagattctt
     8281 tatgataaga ttgcagtaga acagccagtt gaacagccaa ctgaacagcc aactgaacaa
     8341 ccaactgaac aaccagtcga aaccccagca gaccatccag ttgaacagcc agcagaccag
     8401 ccagctgaag ttccaactga acaagcagtt gagcaaccag ttgaacagcc agctgaacaa
     8461 ccagttgaca ctccgacaga caaaccagtt gaacagctaa aagaagcctc gtccttaact
     8521 gaccaggcag ctggcagtgt tcaaacagga aaagccactg acaagaaaaa gatagataat
     8581 tctactccaa gcactcctgc tgttaaacca gaggaagtat cacaacctgt tgccaaaacc
     8641 ttgccaaaaa caggtgatag cagctctatg ctcctagtct tagggggcgt cttgtccggt
     8701 ctttcaggtc taggattagc tgccacatca cgcaaaagag actagtccat ctatgtatac
     8761 caaggaagct ctcatttgag ggctttttct attgtatata tttccattgg caagaaaata
     8821 ctagctgtgc aggatttggc tttgctccct ttttcctagg aaatttggta taataggaac
     8881 aagtacacac aaaggagtct tcattgcaag gaagaattat caaggccttg gctggttttt
     8941 actatgtcga ggcggatgga cagatttatc aaaccagagc tagaggaaat tttcgtaaga
     9001 aaggccaaac gccctatgtg ggtgattttg tggatttttc tgcggaggaa aactcagaag
     9061 gctatatatt aaaaattcat gaacggaaaa atagtctggt tcgccctcct atcgtcaata
     9121 tcgatcaggc ggtggttatc atgtcggcca aggaacctga ttttaacgcc aatttactag
     9181 accgtttcct tgttcttttg gagcagaagg acatggatcc cattatctat atctctaaga
     9241 tggacttggt agaagaacgg gcggagatgg atgccttcaa ggcaatctat gagaaaattg
     9301 gctatccttt tgtctatagt ttggaggaat tgacgccatt attgcaggat aaggtgacgg
     9361 tctttatggg gcagacaggg gttggcaaat cgacacttct caatcgcatt gcgccagaat
     9421 tagccttgga aacaggggct atttcagata gtctggggcg tggtcgccat accactcgtg
     9481 cggtcagttt ctacaatgtc ttcggtggga aaattgcgga tacgccaggt ttttcatcgc
     9541 tagactatga agtcaaggag gctgaggcac tgacagattg tttcccagag attgcggaag
     9601 ccagccagaa ctgcaaattt aggacctgta cccacaccca tgagccggaa tgtgcggtca
     9661 aggaagctgt tgccagctct gccatttccc aaagtcgctt tgacaattat ctccaattcc
     9721 tcagcgaaat ccaaaatcag cgtgaaacct ataccaaggt aagtaagaaa ttcaaataga
     9781 aagttggttc tgtatgtcac attataagat tgcaccgtct attttggcgg cagattatgc
     9841 aaattttgaa aaagagctca agcgtctaga aaagacaggt gtggagtatg tgcacattga
     9901 tgtcatggac ggtcactttg tgccaaatat tagctttggg gcggatgtgg ttgcggctat
     9961 gcgtcctcat agcaagctgg tttttgattg ccatctcatg gtgtccaatc ctgaaaatca
    10021 tatcgagacc tttgctcgag caggtgcgga tatcttgact attcacgcag aagcaacggt
    10081 tcatctacat ggaaccctac aaaaaattcg tgcggctggt atgaaggtgg gtgtggtgat
    10141 caatccaggc actcctcttg tgaccattga gcctgtactc aacttggtgg atcaggttct
    10201 gctcatgacg gtcaacccag gttttggtgg acaagcctac attccagaag tggctgagaa
    10261 gattgcagat ttggttaaac tccgtgaagc caaaggcttg aactttgaca tcgaagtgga
    10321 cggcgggatt gacgacaaga ccattcattc agccaaaaat gcgggtgcca atgtctttgt
    10381 ggctggatcc tacctcttca aaggagactt ggatgccaac gttgccaaat tgagagctgc
    10441 cctccatgat taagattgct gtcattgcag gcggttcctt tgactgcctt cctgagcctg
    10501 ccgacctcta tgtgggggta gatgcaggct ctcttcgtct tctggaccat tccctgcctc
    10561 ttgactgggc tatcggtgat tttgactcgg tgacacctga agaattaggg cggataacgg
    10621 atctagcaga gcgttttttg caagctcctg ctgaaaaaga tgatacagac ttggagctgg
    10681 ctttaaagga aatctttaag gcctatccgc aggaccaggt tcgcatatac ggagccttgg
    10741 gtggtcgcat ggaccacatg atggccaatc tctttctggc tgcagagccg gacttggcac
    10801 cctatatgga gcagattgaa ttggtggata gtcagaacat cgtccgtttt cgatctgcag
    10861 gtcagcaccg tttggcaccg attgctggta tgaagtacat ttcctttatg ccgtcggacc
    10921 agagtcgtct gaccattcgt catgccaagt acccgctgga tgccagcaat tattttttca
    10981 aaaaatgcta ttcctctaac gaatttatag atagagacat agacattcaa ctggataagg
    11041 gctacgtggt ccttatctac agtaaggaca aggattagat ggatattgta ttacttattt
    11101 tgctgatact ggtcttgctc gccttggttt ttctttatgg aaaatggcag agcttggccc
    11161 tgcttttaca agatcaagcc gaagatactg cagacaactt gtctgatcag ctcagctatc
    11221 aactagaaaa cgctaccctc aaacagcaac aggccattca tcaggaagtg gaaagactcc
    11281 gcacggagct ttaccaacag ctaaccgaca tccgtcaaga gctgaataag agccacttgg
    11341 aaacgcgaga tgccaccgac cgccgtttgc aggctattca ggagtccaat gaaaaacgcc
    11401 tggaagaaat gcgacaaaca gtcgaggaaa agctggagaa aaccctgcaa acccgcctac
    11461 aagcctcctt tgaaacggtg tccaagcagt tggaatcggt caatcgtggt ctgggcgaaa
    11521 tgcagacggt ggcgcgtgat gtgggtagtc ttaacaaggt gctgtctggc accaaaaccc
    11581 gtggcatcat gggtgaatta cagctgggac agattatcga ggatatttta acacctagcc
    11641 aatatgagcg agagtttgcc acagtttcag gctctagtga gcgcgtggag tatgcggtca
    11701 agctaccagg acggaccgag ggagactata tctacttgcc aatcgattcc aagtttccgc
    11761 tggctgatta ttaccgcttg gaagatgcct acgaaagtgg ggacaagggc cagattgacc
    11821 tccaccgcaa aaatctctta gcggctatta aacgttttgc caaggacatt cagagcaagt
    11881 acctcaatcc gcctgaaacc accaactttg gtgtcttgtt cttgccgacc gaagggctgt
    11941 attcagaagt ggtccgcaat ccgattttct ttgatgagct tcgccgtcag gaaaatatcg
    12001 tggtagcagg accgaccacc ctgtctgccc tgctcaattc cctctccgtc ggctttaaga
    12061 cccttaacat tcaacggtca gccgatgata tttccaaggt cttgggcaat gtcaagctgg
    12121 aatttggcaa gttttctgac ctcttagtca aggctcagaa acaactcaat caagccagca
    12181 gcaacattga caaactccta accactcgta ccaacgccat cgagcgtagt cttcgtacca
    12241 ttgacctgta cgaagatgac caaaccaagg gactgcttgg cctgtcccca ttagatgagg
    12301 aagataatga aaattaacca aatgaaaaaa gatgaattct tcgaaggatt ctatctaatc
    12361 aagtcagcag aagtccgaca aacacgggct ggaaaagact acctagctct gaccttccag
    12421 gatgatactg gtgagattga aggcaagatc tgggatgccc aaccagggaa aaccaaggat
    12481 tttactgctg gaactgtagt tcatatgcag ggacgccgtg aagtctacaa taatacaccc
    12541 caagtcaatc aattggttct ccgtttgcca aaagctggtg aacccaacga ccccgctgag
    12601 ttcaaggaaa agccacctgt gaatgtcaag gataccaagg actatttgag ccagatgatt
    12661 ttccgcatcg aaaatgccac ttggcagcgg atcgtccgtg ctctttacag caaatacgac
    12721 aaggaatttt attcctatcc agcggccaag accaatcacc atgccttcta ctcaggcttg
    12781 tccttccata cagcgaccat ggtacgattg gcagataaaa ttggtgacat ctatccccag
    12841 ctcaacaaga gcctgctctt tgcaggaatc atgcttcatg acctagccaa ggttatcgaa
    12901 ctaacaggac ctgataacac aggctacact gtccgtggta atctgattgg gcacatttcc
    12961 ttgattgatg aagagattac caaggtcttg atggaattgg gcatcgatga cagtctggag
    13021 gaagtgaccg ttctgcgcca cgttatcctc agccaccacg gcctcttgga gtatggtagc
    13081 ccagtccgtc cgcagattat ggaggcggaa atcctccaca tgattgacaa catcgatgcg
    13141 gaaatgatga tgatgctatc agccttggac aaggtcggcc caggcgagat gaccaaccgc
    13201 atctttgcca tggacaaccg cgccttctat aagcctaacc tagactgatg gcagttagga
    13261 ttgtatctag acagcccctc accaaaggct ggtccacgga ccaaaagtac aaggtccagc
    13321 tagaagatgg tcgctttggt cttttgagaa tagcagagcg accagcctat gaggctaagc
    13381 gattagagtt tcagttagtt gaaaacctgt ttggtctagg tttgcctgtg gcagagccac
    13441 taagtttttg ggcggatgac ttgtcagtct acacccttta tgaatgggtg gaaggtcagg
    13501 atatgaatga agtggcttct agcctgtctg attctgtctt gtatgaattg ggctgtcagt
    13561 cagggaagtt tttgcgaacc ttgcatgccc taccaattga ccaaagtcaa cgggactgga
    13621 acagttttta tcaggttaag attgacaata aattagctgc ctaccaagca gcgagtcatt
    13681 cctatccaaa tggtcaagct atgatagact ttgttcaggc caatcgtcgc ttgcttgaag
    13741 ggagatcgat tgcctaccac catggagatt ttcacacagg taacttcctg ctgggtgcgg
    13801 atgggaaact gaaaatatta gactttgatc gctacgatat aggagacccc tgggaagaat
    13861 tcaaccgttt gattttcacg gcagatttgt ctccagcctt tgcgcatggt caggtagatg
    13921 cctattttga cggcgctatt ccagaggaat tttggaaact catggctctt tatgtaacag
    13981 taaatagtct aggcgctctt tcttgggcag aacaagtaga ttccgaacaa attccattga
    14041 tgaagttgca agcagcaact atctcagaat ggtatgcgga ctttacttgc tggattccga
    14101 cttggtatgg gcaagtctat ggctaaatac gaaaaagcga acttttttta tgaaaaaagt
    14161 tcgctttttg tttttcttgt gttataatag gaaaaagcca aaaaggagaa cgatatgaaa
    14221 ttaagaagaa gtgagcgtat ggttgtcatt tccaattacc tcatcaacca cccttatgaa
    14281 ttaactagtt tgaatacctt tgcggaaaag tatgagtctg ccaagtcatc tatttcagaa
    14341 gatattgcta ttattaaaaa agcgtttgaa gaaagttcga ttggtcaaat tgaaaccatc
    14401 acgggagcta gcggaggggc tgtgtttaca ccttctattt ctaaggaaga gtccattgag
    14461 attgcacagg cccttcgtga tcagatggcc gaaagtaatc gtatcttgcc aggcggttat
    14521 atctattcat ctgacttgct gtctacacca catattttga aaaatgtcgg tcgtattatt
    14581 gctaatgctt ttaaggaaga aaagattgat gctgtcatga cagttgcgac aaagggggtc
    14641 cctttggcca atgctgttgc taatgtcctt aatgtaccgt ttgttattgt gcgtcgtgat
    14701 ttgaaaatca cagaaggttc aaccgtttct gtcaactatg tgtcaggatc gagtgaccgt
    14761 atcgaaaaga tgttcctgtc taagcgtagt ttgaaggcag gaagtcgtgt cttgatcgtc
    14821 gatgatttct tgaaaaatgg gggcaccatc aatggtatga ttagcctctt gtctgagttt
    14881 gactcaacct tggtaggcgt ggctgtcttt gcagaaaacc aaaaagggga ccgtagcgta
    14941 gctaactaca agtctttgct cgctgtgacc gacatcaatg tcaaggaaaa tcgcgtggat
    15001 gttgagttgg ggaatatatt tgattaatcg ttaagcctct cttgatgaga ggttttttgt
    15061 tgtttgtctg ctagggctta acctgttata ataaatatag attggattct gagaggttga
    15121 tatgaaagtt ttactagcaa caagattaaa aaatagaaga aaagaattgg gttggtcgca
    15181 gaaggagttg gcagaagggg tctgtgatca agggcagatt agccgcattg agaagggaac
    15241 ctacatgcct ggtgcagact tgcttcatgc ccttgctaaa aaactccaag tcagaatgga
    15301 ctattttttt gatgaagagg aaagcgaaat tgtctctgac ctgaaacagt ttagaaaact
    15361 tgccaaaaac tgtcttatgc aaagagatta tgaggccttg ggttttctat atgaaaggga
    15421 aaagggtagt ctatctcatc tgacctttcc agaccaagtt tatttggaat gggtggaggc
    15481 tgttttagca gagtatttgg aagcaaggcg agaagatgct attcagaaat tgagggagtt
    15541 gctggaacga gttggggaga ataggagagg ctacctctat ctatacaata gtttattgat
    15601 tctattgatt cgagatgaga agaatgctga ggcagagcaa ttctatagtg aggtaatcga
    15661 aaagattgat aaaaataagt tggatttatt ggtagatata gatgcttact tcagtattca
    15721 gtccaactac atccgctacc tatggttgac aaatcagttg gaaaagggaa ttgagctggt
    15781 tacagcctgc attgaggaat tcaagtcaat ctatcctatc catttcttgg cagattttta
    15841 ttgtgactta ggaaatgtga cagaagactt tgcggataag caactggtca aagaacgcta
    15901 tgaactatca cgacttcttt acgagttagc aggcattgat gctattgctt tgaaaataga
    15961 aaaatacctc aaagaaacct atacagattg agcttgctcc agaggggcag gcttcttttt
    16021 atatcaaaaa tataacaata ataaatttaa tcaaaaaaat atcaaaaagt attgacggcg
    16081 agtaacgaaa cggttatact atagaaaaga aaggggttac aaaatgaaaa gaatagttac
    16141 gagtgttttg gtggtgggat tactagcggt tattttaatt agagttccac aattgccgag
    16201 agtttttggt gtagatagag aagagagagt tatctataca aaatagttag ggcggagtat
    16261 atatgtttaa aaaatatgct tgcactttgc aacatgacca gtcagattgt gcagcagctg
    16321 ttgtctccac tgtattattg agctataaaa aagaactttc aataatgaaa attcgggaaa
    16381 ttattggaac agatatgtac ggaacgacag ttcacggaat tgtttctggt cttgaaaagt
    16441 taaattttac cgtaaaggca gttcgagtat ccttggatga cttaagtgga gatattagct
    16501 ttccagctat attacaaata cggaatagtc tggggcagaa tcattttata gtgattcata
    16561 agataaagaa agatggtaat tatttggtag ctgatccagc aaaaggactg gatatgcttt
    16621 caattacaga acttgaggaa gtttatcaag gtatagccat tttcatggtt ccaaatagtg
    16681 agtttgaaaa agggaacatg aaaggcaaag gactatttga cttattttcg gctctgatat
    16741 taacacagaa aggacttgtg gagacaatca tcttagcatc attcttgcta agtatcgttg
    16801 gtattttgtc tagtcttttt tccaaagtta ttatggatga gattattccc tttggattaa
    16861 agaacagctt atacttattc ttgattgttt ttggcatcgt ttctttagtg cagagtctct
    16921 tatctgcttt taggcaacat attttgctct ttctatcaag aaaaattgat attcctgtgc
    16981 tcctgggcta ttatgaccat attattcacc taccttatag tttttcggta gcagaagggt
    17041 gggtgatgtc ttgacacggt ttcaagatgc catgacgata aagaatgttt ttacatcggt
    17101 ttctatttcg ttagttatgg atattagcct atcactgata agcgcctttg ttttatggca
    17161 cctcaacccc actttatttc taatattggt tatgatggtg attgtgaata tcgtgttaat
    17221 ttattgcttc aagaaacctt ataagaagat taataatgag cagatggaag caaactctat
    17281 gcttaattcg caattgatag aatctattcg taacatagat accataaaat cacagcatga
    17341 tgaacgacaa cgtttggata aactagaaga gaattttgtt cacaccttag aaattggcta
    17401 taaagaaggt gttttacaaa atattcaatc tacaatttct tctatagccg gaactctagg
    17461 gaatttgatt tttatgggag tgggagcctt atttattatt gataacaaga tgacaattgg
    17521 tgatcttctt gtttttcaga ccttgagtca gtattttact gagcctgttc agaatttggt
    17581 tggtctccaa cttactttcc aggaagttca ggttgctgtt agccgacttc aagaattgat
    17641 ggatgttgag cgcgaagata gtcaagtagg gaatagtatc cataattttt cgttattgga
    17701 agatattgtc ttcaaaaacg tttctttcgc ttatggatct agaccgccta ttattcgaga
    17761 ttttagtttg acaatcaaac aaggtgaaaa aatagctttt gtaggtgaaa gtggcgcggg
    17821 aaagagtact cttgtacgac ttcttcttca tttcattcag cctattgaag gtcaaataaa
    17881 gtttggtgag tatgacttag gggatttaga ttatggagaa ttacggaaaa gaatagctta
    17941 tatccctcag acagttgagc tcttttcagg gactataatt gataatttaa agataggaaa
    18001 tccagaagct agctatgagg atatgatacg tgtatgccgc atagtaggga ttcatgacac
    18061 aattcaaaaa ttacaaaacc ggtatggttc ttttgttgaa gaaggtggcc agaatttctc
    18121 aggaggagaa aaacaacgtc ttgctattgc ccgagctctt cttagtaaag cagatcttta
    18181 tatttttgat gaagcaactt ccaatctaga ttcctttagt gaacagatta tccaaaattt
    18241 aatatttgaa aaaattattg gcaagacgac agttgtagtg gcccacagat tatcaacgat
    18301 tctacgttgt gataaaatct gttttttgga agacgggaag attgtagaat atgggacgca
    18361 tgatgagttg atgaagttgg gtggccgcta tgcaacaatg gttggcttac aaagaatcca
    18421 actagataag caagaaaaaa cagttgctac tagctggtca gaagaggtag tatatgagta
    18481 agttagaagt attagaaaac aaaaaattag ttttgaaaaa agtgatttgt aagaagctaa
    18541 gtagccttca gtttgaagac ttggaacaag aaatagataa gttttatcag cacttacaat
    18601 tactgaaagt gcaaatgttt ggccctttga ttgtaaaaaa tagtggaact attcttcatg
    18661 aagatgggac tattactgta gactatgatt tgtatgtcca agctcatgat tttcgccagt
    18721 atgataagtt ttatactgtt tatgaagaaa ttgtttgtcc acattgtgtc tatgtacgat
    18781 ttgaagatag tccagagtat ttgcaatatg catattctaa actagattta catttctatg
    18841 aaaatgattt ggaaacagat ggcattattt acaccgtcta tgtcaataca aataatgaaa
    18901 ccatgacggt ggatattttt agaccgatta ttagcttatg aaactatata acaaatctga
    18961 acttcgttat tctagaatct tcttcgataa gcgtcctcca gcctacgcgt ttatcctaat
    19021 ctttagtaca ctagctactt tgattatagc ttttgcagta tctgcctatt tgcctaaaaa
    19081 ttacatagtc aaagctaggg ggacttccgt gataacagga acagagtatg tatctgctct
    19141 gagcaatggg aagattgtga cacttcataa aaaggaaggg gatagtgtaa aagctggtga
    19201 ggttattctt tctctatcta gtggacaaga aggacttcag actgaatctt taactaaaca
    19261 attggacaag ctcaaagcta aagagagtct ctttcaaaag tatgaacagt ctctgaatga
    19321 aaaagttaat tatatggcaa attcaggtga agagcaggag tattacggga aggtagaata
    19381 ttatctatct caacttagct cagaaaacta taatgatggt agtcaatatg ctaagttgca
    19441 ggaagagtac actaagttga ataaattagt ggcggagaaa agtcaattag aaacggactt
    19501 gtctagttac caatcgcaac ttgctaattt agaggcagaa ttggcaactc tttctgttca
    19561 agagacagca gaaacgaatt ccgatagtga gaccagttca tcatccgttg acacttcttc
    19621 taatagcatt cagttggagc agctaaatgc aaaaatatca gaggtcaaaa caaaaattga
    19681 aacgactact tcttctatac aaggaaaggg gacggaaata gagtccctac agtctagtat
    19741 tagggagatg gagcgttctt acaatgatcc tacttctcaa gcttacaaca cttatgccca
    19801 gttaatcagt gagttaggaa cagcaagggc ggccaataat aaaagcataa cagagttaga
    19861 agctaattta gggattgcca caggtcagga tagagggcta actgtttctg cctccagtga
    19921 cggaactcta cattatgtta ctccgctcaa gcaaggcatg tctgtccaac aaaatcagac
    19981 tgtcgctgaa attgctggta gtgatagaga agcctatgta gaagcctttg ttcttgcgac
    20041 agatatttcc cgtgtaacag tcggtgcaga ggtagatata gctatcacag gtgtaaacag
    20101 tcagaaattt ggtaccttaa agggtgctgt aactcagatt gactctggaa cgattactca
    20161 ggaaactcag aatggtaatg ttagttttta caaggtggtt gttgctcttg aagaattgac
    20221 tctaaaaaaa gatgaagaaa ttgtagtttt acaaaaagat atgccagtgg aggctcgtat
    20281 cgtttatgat aatgaaacct atttggattg gattctagaa ttgttgagtt ttaaacaata
    20341 gtggttttat atctttacag tagataaatc caaacagtat ggatgaaagg tccttctgct
    20401 atctaggtaa agaatgagaa acgttgatta tatccagtta gaggattgtg tctaactatg
    20461 attttttaaa ggagaatttt tatggaatta gtaatgccga ataattatgt agttcttgaa
    20521 gaagaagaaa tgatgtacct tgatgggggt gaaatagcca cagctactgt attagggata
    20581 atatcagctg ccgtagctgc gggtggagca gcttatggag ctggcttagc tgcaggtact
    20641 agagtatact atgcagggtt aagaaattcg caatatcaaa aaatcaaatg gcaagttcgg
    20701 gcggcggcac tagttgttgg aaatgtttgg ggaggtattt ttatgaccgg atttgaaaat
    20761 gctttctatg caaaagtaac tgggaagtaa tcacttatga aatatttacc tttgttctta
    20821 ttcatagaag gcagtattgt tcttttgtta tgcggtacaa tatatttttc gcaaaatgag
    20881 tttaatcagt tatcgggggt tgcacttatt gttacattag cacaaatttg tatgctaatt
    20941 tcttattact tattgataaa agcaaaatag ctttgtatta aaaggttgta acattatttg
    21001 tcaataattt atttatgaag ataaatctaa tggtactatc tttataaaaa aatattaaag
    21061 aaacctattt ggattggatt ctagacttgt tgagttttaa acaataatgg ttttatatct
    21121 ttacagtaga taaatcccaa cagtgtagat gaaaggtcct tctgctatct aggtaaagaa
    21181 tgagaaatgt tgattatatc cagttagagg attgcgtcta accatgattt ttaaaggaga
    21241 atttttatgg aattagtact accaaataat tatgtggctc ttgaacaaga agaaatgatg
    21301 tatcttgatg ggggagattg gaatacattt aaaaacaata taaaaggttt ggcagcttct
    21361 tcagcaggat ttagagatgc tgctcgacaa gtaggcttat ggggggcaat ttggtctgcg
    21421 agagtgtata gctattctta tgtagtttct gttttggcac caatactcgg ttcagtaact
    21481 gcggctaatt ttgttttagg agctattggc gtagctacag taggagccgt tagttatgct
    21541 ctatggaact ataaatatta ttgattaata tgctcatttt tttaagattt ttattaatgg
    21601 tttcgctgta cggattatgg ctagagatac atttattatt tgtttctctg gcatttaatc
    21661 tagaaccatt aacaggttat ttattaatta catgccttac ttttttaact atatttttag
    21721 tcaaaatatt acttgatttc ttatatttaa ttaagagtct ataccaaaaa gagtgcattt
    21781 ttaccgtctt tccttttatt cttataaaaa gacagaaaat gaatattaga ctttctccat
    21841 acttttcatt tcatcgtaaa tatttatcta atagtagttt aaaatatagc ttactatcat
    21901 tatttctttg tgtaatcgct ctgatgacaa caatcatatt aaaaaagccg attctatcta
    21961 ttgtctttac tgctatattg ttttggacta tagtagaaat tactcatata atttttaatc
    22021 caatggaaat catttttact gattataaat ggaaaaaagg ggacttgttt gatagtgttc
    22081 taagtaagtt gttaaagaat caaatccaaa cttcagaaat cagttactct aatctaatgt
    22141 ctatccttct ttatgatgcg attaatcaat gtacttttct aactgatggt gatttttttg
    22201 aagatatttt ttcaaaaatt atggactctg aagatattgt cttgtgtaca ggtcttgttg
    22261 aactattaat gtatgaaggc ctcattggaa aaaataataa ttacttagaa aaatgtgaaa
    22321 agattagaaa tcatcttatt atgattgaac gattagattt tttctgttat acgagttggc
    22381 taaaatataa ttttgattta tgtgtaaatc gagagtatgg taaaattgaa gttcgtaaaa
    22441 tattaattag taataaaaaa ataatataat ttattattca ttaactattt caaggtagta
    22501 ttctttacaa attggaaatc aatagagatg ttgtgtaaga tatctatgat ggctttgccc
    22561 tcatccgtaa aagaataaaa acgaacagga atccctcact aggtgtataa tcttagtgag
    22621 ggatttgagg tttatgtgat ttttagtctg tcacgcgcct tttcaaaact ctccgacctt
    22681 gcttctacac tgatttcttc taaagtcagc gggtgggtca ggctgagttt ttgggcgtgg
    22741 agcatgaggc ggggggctgc aactcgactg taaagtgggt cgccgattag agcatgaccg
    22801 tggtgggcta gatgaactcg aatctggtgg gtccttcctg tctggaggcg acaagagacc
    22861 aaactatttt taccaagggg cttcaagagg cggacctggg taagggctgc ctgtcctttg
    22921 cgattatcga ccatccgctt tctacggtca tgcctgtccc gtccgatttt atcggtaatg
    22981 gtccagtctg tctcttttag ctggccttgg cagagggcta ggtagtcacg gtagatgacc
    23041 ttgtcttcca agagccgatt gagaatgggt aggataaaag gatttttagc aaagagaatg
    23101 gctccgctgg tttccatatc gagcctgtgg actacatagc aggtctggcc gacataggca
    23161 gagacatggt tgagcagggc aatttccgtc ggctcatttc catgggtctt catgccttca
    23221 ggcttgttga cgataatcaa gtgctcatct tgatagagtt cctccaccaa gttcccctgt
    23281 cccataggaa tgcttttttc agggtaatct tcctggtcaa atgtcaattg cagcaggtca
    23341 cctgctgcaa ttgggctctg ccagttaatc agctcaccgt tgacacggac atgcttcttg
    23401 gtgcggagga agtggcggat ttttctggga atgagaaaat aatcttccaa gacctccttg
    23461 acagtcagtt ggggaaaggc ttggggaatg cgaatgtcaa ttttcatctt ataggctggt
    23521 tgagtgcttt ggttgcactt tttcgtttgg attgaggtag ttcatggcat tgttgacagc
    23581 gattggggct tcgcccagtc cagtcgcaat caggtcaatc ttgccttcgt agaagcaaca
    23641 gtcaccgatg gcatagatac cagggacaga ggtttcttgc ttgctgttga ccacgatgcg
    23701 gtgacgattg agttccaatc cccattcttt cagcgttccg acagatgatt tgaaaccgta
    23761 gttgacaaag aggtggtcaa aagacagggt cagcttgtcc tcgcttttta ccttatcgaa
    23821 atcaatggca gaagctctgc cattttcccc agcaagccct ttagggacaa atggtgtgtg
    23881 aatggtcacg ctggactgct tgagctcttc cacgctgtgc tcgagggctc ggaagttgtc
    23941 gcgacgatgg acgatttggg tagttttggc gattttttca aaggccaggg accaatcgac
    24001 cgcagagtca ccgccaccca agacgacgat gtccttgtcg gcatactgct ggatattaga
    24061 tacgtggtag tggacgttgt caaagctgtc ggcaccgtca atctccagcg gacgaggctt
    24121 gaaggccccg ccacccatgg cgataatcag ggtcttggtc tggtggcagc ccttgttggt
    24181 tgttagggta atggtttccc caggttcgat agcagtcagg gtttcattga ggcagatggt
    24241 ggtgtcaaat ggagccagtt gggcaaggag gttgtcggtc aactcctgtc ccgtcaggct
    24301 tggaaaggcc ggaatgtcta aaatggcctt ctcaggatag agaatggcag gctggccacc
    24361 cagttgggga agggagtcaa taattttgac cttggcttga cggagatgag cgtagaaggc
    24421 ggcgaagaga ccgactggtc cgccaccgat gatagtaata tcatatactt gtgtcatggg
    24481 aatcctcttc tttgttcaga atgaccctat tttatcatat ttttggaaaa ttttctctgt
    24541 ctgttgtcac aagtggtcgc aggctttttt ctgctagttt ttaggaaatg gtgtataatg
    24601 gtacaaaatt cactagaaag aggtagagta tggacgattt gcaacatcag ccgattgaag
    24661 ttccagaaga tttccaaatt gatcatgaag accgacctag ccgtcgcagc aggggaaatc
    24721 gtggctctgt tgataaacca aagaaaaaat cacgtattcc ggagccgatt cgtaaatttt
    24781 ggcgccgtta tcagttgacc aagattttga tgattctggt aggagttctg attctgacag
    24841 ttggtggcta tctcttctat ctggccaaaa cagccaacgt cggtgattta cagcaggcgc
    24901 taaaagccac aacgattatt tacgataaag atggtgcgga agctgggaca ttgtctggtc
    24961 agaaggggac ctatgtagag ctggatgctg tctctgataa tttagagaat gccgttatcg
    25021 ctacagaaga tcggagcttc tacaaaaatt caggcattaa ttaccagcgt acaattttgg
    25081 ctgccttgac gttaggtcgg tctggaggtg gttcaactat tacccagcaa ttggcaaaaa
    25141 atgcctttct aactcaggat cagaccatca gtcgaaaggc tcgagaattc tttttggctc
    25201 tggaaatcaa taaaaagtac agcaagcaag aaatcctgac catgtatctc aacaatgctt
    25261 actttggaaa cggggtctgg ggagtggaag atgcaagcca aaagtacttc ggtatttcag
    25321 ctagtgatct tactcttgag caagcggccg tcatagcagg tatgctcaag ggacctgaga
    25381 tttataatcc ttattattct ctagaaaatg cagttaatcg tcgggataca gtcctgcaaa
    25441 atatggtcaa tgctggttat attgatcagg cgactgcaga tgcgggtttc caagtagacc
    25501 tagccagtca gctttcagat acctatacag ggaaacaaag caattattcc tatccatcct
    25561 attttgatgc tgtgattgct gaggcgattg agcgatatgg attgaaagaa tcagacatta
    25621 ttaataacgg ctataggatt tacacagaga tggatcaaaa ttctcaagcc agcatgcagg
    25681 tgatttttaa tgatgagacc atgttcccaa cgtcaagttt tgatggtacc catgctcagg
    25741 ctgccagcgt agctcttgat cctaggactg gtggagttcg cgccttggtt ggtcgagtta
    25801 acagttcaga ggatgcggtc tttcggactt tcaactttgc gacacaagcc aagcgcagtc
    25861 ctggttcaac aatcaagcct cttgttgcct atgctccagc agtagcgtcg ggctggtcga
    25921 ttgatatggc tctggataac catacagaaa cctacggcga ttacaccttg cataactatg
    25981 attattcgac ttctgatacc attccaatgt atcaagcttt ggctctctct tacaatttac
    26041 cagtagctta tatagtgaac accttgggga ttgacaaagc ctttgagtat ggacaaaagt
    26101 ttggacttga tatggaaaat gtcaagaaag ttttgggtgt gtcaatcggt tctggagtgg
    26161 agactaatcc tctgcaaatg gcccaggcct acgctacttt tgcgaataag ggtgtgatga
    26221 aggatgccca cctcatcact cgtattgaaa cagctagtgg aaaggttttg gcagagcatc
    26281 gagaaacaag tagtaaggtg attgaccgct ctgtcgcaga taagatgaca gcaatgatgc
    26341 ttggaaccta taccaatggt acgggagtga cagcagatgc ggccaattat tatattgccg
    26401 ggaaaacagg aacaaccgaa acgagttttg atgtcaatct tgtcaatgat cagtggcata
    26461 ttgcctacac acctgatttg gtcatcagtc agtgggttgg ttttgagcag acagatgaaa
    26521 atcattatat agatagcagt agctactgga tggctccgtc ggtcttccag actgtagcca
    26581 attcaatctt gccatatact gcaggaactc agttcgaagt agaaaatgcc tatgcacaaa
    26641 atggtattgt agaagtagct ccggatgaag ctgcgcaaac tgaaccaagc cttggtcaag
    26701 aacaggtaca agaaatttca gatcgggcga aaaacttgat tgagcaaacc aaacaaaatc
    26761 taatcgatgc ccagttacca gaacgagcaa aaaccttgtg ggataacatt acgtcttggt
    26821 ttggagagtt gtcaaatcca taaaaacatg gtagaatgaa aaggatggag ggctttatgg
    26881 cactaaaaaa agcaagccta gcctgtactg tttgtggttc tcgaaactat tcaatcagcc
    26941 tttcgagcaa tcccaagcca acacggttag aagtaaataa attttgtaaa cactgtggac
    27001 aatacactgt tcacaaggaa accagatagg agtcgcgcat gaaatttatc aaagacatta
    27061 tccgcatttt aaaagatacg tcttggccaa ctcgtaagca gagctggacg gatttcgtgt
    27121 ctgtagtgga atatactgcc tttttcgtag cggtggttta cctctttgac ttgattttgt
    27181 cacgaggaat catgagcttg atcaatcttt tctagaaaat acttgccatt tgtaaaatgg
    27241 aagtgatata ataaggaaaa gcgaaagcct tcgggctttt tttagcgtct aaaaattccg
    27301 tgtttccaaa gaaaggaagt aacaatgtac gatagttttg ataaaggctg gtttgtttta
    27361 cagacctact caggatatga aaataaagtg aaggaaaact tgctccaacg tgcccacact
    27421 tataacatgt tggaaaacat ccttcgtgta gaaattccaa ctcagactgt tcaggttgaa
    27481 aagaacggtg aggtcaagga agtggaagaa aaccgtttcc ctggttatgt tttggttgag
    27541 atggttatga cggatgaagc ttggtttgtg gttcgtaaca cgccaaacgt tacaggtttc
    27601 gtgggttcac acggtaaccg ttcaaaacca acaccacttt tggaagaaga aattcgtcaa
    27661 atcttggtgt ctatgggaca aactgttcaa gaatttgata ttgatgtcaa agttggcgat
    27721 actgttcgta tcatcgacgg tgccttcaca gactacacag gtaaaattac agaaatcgac
    27781 aacaacaagg ttaaaatggt tatctctatg tttggtaatg ataccattgc tgaagttaat
    27841 ttgagccaga ttgctgagtt gtaataagtt gttaaagcgc agggaagcct gtgctttttc
    27901 ttgtttttga gggctaattc aaaaaaactt gccaaagatt taaaaatttt atataattag
    27961 gtatacctaa cttataaatt aattaatctt aaaaaacgga ggttttatga aacagaagtg
    28021 gtctcagatt gagaataaac aacgctttag tatcaagaag ttaagtgtcg gtgtggcatc
    28081 ggtatcaata ggatttttta tcacgggtgt tccgatggtt caagcagata ctagtggaga
    28141 aggattggag tctacagttg cggtcgcaat ggatatggat agtaggcaaa attctgcggt
    28201 agagaaaaaa gaagatggac cattatcaga tgagccagtt aaaacggagc aggtggatga
    28261 accagttgtt gaagagggag tggtcgaaga agttgtagac acagaggcag atgaagagcc
    28321 ggttctttcc gtagatcgag ctgcgaccga tataaaaact acagctggta agacaacaga
    28381 ggagtcaaag gaaaaagaag acattagcgg caaagaagct agtgctcctc aaacaatccc
    28441 gcaggaatca cagcttgagc cagaagaggt gacaacaggg cgctatattt tacaattttc
    28501 cgaagagaat cgaaatcttg tattggataa attaaagaaa attgatgacg ttaaaattgt
    28561 tcatgagtat aaggaggttt taacgggagc atcagtagag gtaggaaaag aaagtttgtc
    28621 tgatgtgaag gcaattaccg aattaacctc tttagaagag agtcgccgta ttcgaccgac
    28681 tcttcatact gctaaacagc tggttggtgc cttaaaagca agttctaaat atcagacaga
    28741 tggtcgcgga atggtgattg cagtcattga ctccggtttg gatattaagc acaaagacat
    28801 gaggttggat gatggtgtca ttcctaaaat taaggacatt actccatcta cgacaggaac
    28861 atacacactg aaagttcccc atggctataa ttatgtatct ggcaacgata atctctacga
    28921 tgatacccac gaaccacatg gtatgcacat agcaggaact ctggctggaa atgcaacaga
    28981 cgaagaagtt gcgtctaaaa agggagttga tgggattgct cccaacgcac aactgctagt
    29041 ctacaagatt ttttcaaatg accctaaaaa ttataaagct gagacggagg atgctgctta
    29101 tgctgctatt gaagatgcta tcaaacacgg ggcagatgtc attagtttga gcgttggtta
    29161 ttatgatagc gggctaccag ggaatgctta ctacacgatt gctaagaggg cagcagaaaa
    29221 gggaattatc attacagcgg ctattggtaa tgctggtgcc tcctcttcag acacctcctt
    29281 tgacttgcat acaaataatg ccttaggagc tgtggatacg gcaacaacag ttggtgtggc
    29341 agcgactccc gcagttatcg gggttggttc ggcgagaaat acccatcttg ttcaacgtga
    29401 atttatgctg aatggacagt cttttggcta ctatcctatt ggctatacaa cgcttacaga
    29461 gggaaaatat gaatttgtag atgctggaaa tggtcgttgg gaagaagtgc aggggcttga
    29521 tttggctggt aaagtagcga ttatcaaaaa agataagttt gacttgaaag atgccgttcg
    29581 taatttgaaa ttcaaggatg ttgctggact tgttgtgata aacacagatc aaggatggaa
    29641 caaggattat tataggacgc atcagttatt ggtagacgat aagactttgc tttcctactc
    29701 ctctatctgg ggcatcagtc ttagcggaga agatggaagg cgattattag aagttgccaa
    29761 ccaatcgcaa ggtaacactg gtttggttct taaacctaca attggaatga agaagttgat
    29821 tgaagtgccg actgtatcag gcttttctag ttggggtcct acggtcaact tagaattgaa
    29881 gccagagatt gtggcaccgg gagaggatgt ttatgcaacc ttgaacgaca atcgttatgg
    29941 ctccatgtca gggacttcga tggcttcgcc aattgtggcg ggtgccagtg cacttttatt
    30001 gccacggatt cgccaaatga caccaccaga aggcatgact aggatggatt tgctgagaat
    30061 cattttgatg aatactgcta cccctttgat tgatgttcta gattcatctg ggcatgcttt
    30121 ggaaaattcc ccaagacaac aaggtgctgg tctgttgcag attgatagag cctttgaaac
    30181 agatgttatt cttcaccacc gtctaaaagg aggggtggaa ttaaaagaaa tagggcgtga
    30241 gacagaattt gaggtaacct tggaaaatct gggaaaccaa caaagaagct ttgctatttc
    30301 agcagggaaa gtattgacta gtcaagatgt tcctgttgat agaataggac gttctggaaa
    30361 aatagttaaa gagattcatg cggcagaaat caagggttcg agtattcatc tttctgagca
    30421 atctattcaa ctggggccga aagagaggaa aaccattcgc ctgagactgc atgcaggaga
    30481 agcgaaggac cagtttgcag aagggtatat ttacttcaaa tcattgacgg aggggcagtc
    30541 tgacatcagc attccttatt ttggctttgt gggtgactgg tcaaaagaaa ggattgttga
    30601 tgcaccggct tgggaaacca gttctaaatt gaaactgacc tcggttttat ctagctataa
    30661 acacaataag tctgggcgct atattgaact tgggcgtgaa aagattcaag ataatcaatc
    30721 gcctctcaat ccagacaata tcgcaattca aaaccagcat tcggacagtc agactgggaa
    30781 tgcctttgtt cgatttgcgc tattgaggga tattaccaac tatgatttgg atattgtaaa
    30841 agaagctaca gaagatgctc ctgttttaag gcggattgat acaggaacca tgctgtctcg
    30901 tgttcgctat gcagattatt ttgaaagtct ttcggagtat tccaagcttc gtactccgat
    30961 agaattgcac cgttgggatg ggaaagtgta cgatgcaagc aatgatgaaa atataccagt
    31021 accggaagga caatatttct ttagattgcg ggtgaaaaat aaagaaaatg gggcctatca
    31081 gtatacctat ctgccagtca aaattgataa tcaaaagcca gaaattgtcg cgattaatac
    31141 caatcgtcta accagccata gagaactggt tgtcacagcc aaggataata ataaagtttg
    31201 ggaggttcga gctaatctaa atggagaaaa tcttcttgtt gaaaaggttg tagatgatgc
    31261 aggtcaacta cattaccatc tcaaagaagt ggaactgcca ctcgatgcta aaaatcatct
    31321 ccgtgttgag gtgatggata tagcgggtaa tgtgattgct gttgaaaaag acttagtggc
    31381 gcctgtaatc cagtttaaaa atttggaaga tttgatggca attcgcagta agaaaactgt
    31441 agaaattaaa gcgaacgtct cagcgcaagt ttccgatgta caggctaact tggatgctca
    31501 ggctgtgaat tactctttgg agaatggtca actatctctc cagattccgg atcaatcaga
    31561 tggacgtcat agttttgaat taatcttgaa agataaggat ggcaacccta tttacactaa
    31621 aaccttgaaa tatttggtag ataatgaaaa gccgacgatt gatcttgata ttgaaaaaga
    31681 tgaagaggat gaagaggtta tccagattgg caaaaacggg cgttttacat taaaggggaa
    31741 agtgagtgat aatgttagtc ttcctaagga catcaaactt tactattcca atctagatat
    31801 tggtaaggga gagctgaaaa tcatagatgt aaaagaagac ggcagttttg agcaagactt
    31861 ctttaaatct gattttccta gagccatcat gttaactgcg gtagatgaga agggcaataa
    31921 attaaaggac cttcgtatca atacaagtcc agagagtctg gatgaggagg aggaaacaga
    31981 agttccgatt actgttaata actggttgat tgacccgatt cgtttcaata aagaaagtct
    32041 tggtcgtgag ctagatagcg ggttggtcga cttcaaaaag caagaagatg ggacttatct
    32101 atttacattt gaaatagaag cagaaacgga tcaagctcat tctgtgcgca tcaatggtgg
    32161 tgaaaaacgc tattttgagg atggtaagtt gacctatcca gttaccctga ttgaagaggg
    32221 aaatgttgtg gatataagtg tgtacaatga ggcggatgag ttgacttaca cgaagaaata
    32281 tcagatgttg gttgatacag aaaaccctgt tttacagttg gaaaatgagg tgctgccatt
    32341 ggaaagacag gtagtggata gtgaagaaga tgaagacgag gaaaatcagt atgcaggagt
    32401 tctcctggca gatgctgatg ggcacttgac acttaccgga agcgccaagg ataatggaat
    32461 ttattggtcc ttgaagatca acgaagattt tgtagctcga ggaggcttct ggaggcagta
    32521 tggaaataat gaaaaagcct ttcgctatga gctacatagt ctgaaagacg gggatacagt
    32581 caaacttgat ttgagtgaca gttttggaaa tgctgttgtg aagaaatata aggtacggtt
    32641 aaatgacaag gaagtatctg agcaagttcc tgaaaaagac cttcatgtgg agcaatcaga
    32701 taaggatcaa acccctagca ttcctattcc gaaatcggaa gcgcatgtac caatgccaaa
    32761 agaagataat tcacttgctc cgcaaacaga atccactgaa atcgcgctgt taactggcga
    32821 tacaagagaa gacggtgtgg agcatttggt gagacttaca aaacatgagg aggcactagg
    32881 aatttctgac gagagaatag aagtgtccgt acctcacaga gaattctttg aaagaagcag
    32941 aagaggagag acaggtgcac tagtggcgga tactagtggg aaactgcctc aaactggaga
    33001 tagccttgga agtgtcttta taagtgcttt gttaggtcta tttggtggag ctatggccct
    33061 cggaaatctg aagcgaaaag aatagcaaaa aacctaggga gaatcctagg ttttttagaa
    33121 tattaaaagg cggtagacgg atttgaaccg acgatcaagc ttttgcagag ccatgcctta
    33181 ccacttggct ataccgccat aacaaagaat attctaccga aaaaaaagct gaaagtcaag
    33241 aaaaatctag tgactgtttg tgactgataa caaatctata aaacagttgc gaaaacatag
    33301 gagaatacga cgaaaaagat aggagtactt tttgagtatt gatggctagt accactctct
    33361 ttgtttgata gaaaaagagt ggagcaaaaa gaattaagaa acactttggg taagtgaatt
    33421 catttttact gcaaggaatg ttaagggaat agaattacga taatagtgca cctctt
//
LOCUS       DATUCG010000009        30890 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.009, whole genome shotgun sequence.
ACCESSION   DATUCG010000009 DATUCG010000000
VERSION     DATUCG010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 30890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30890
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.009"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            <1..138
                     /locus_tag="VCA20_000348"
     CDS             <1..138
                     /locus_tag="VCA20_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEP1834215.1"
                     /translation="LPPYSPEYNPIEKTWAHIKKHLRRVLPNCDTFLEALSSCSCFSL
                     L"
     gene            complement(236..889)
                     /locus_tag="VCA20_000349"
     CDS             complement(236..889)
                     /locus_tag="VCA20_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEP1834216.1"
                     /translation="MKNKQIHLRNASFFALAFVILGYVVKFYPTTLAGFDSTVQSVIR
                     GNLPSGASQFWTSITVLGNTVIILAITMILAAIFYYYKKWKIEAYFLLASFAAMGVAS
                     TALKYVYQRPRPSIEWLIDTIGYSFPSWHTASTMMIAGAVVIIVHQRMKKGIARLVLQ
                     AVLILLAVLVAVSRIYIGVHYPTDIIGGWLLAVTLLQMTYPFYDQKRFEWRFQSKQK"
     gene            complement(914..1471)
                     /locus_tag="VCA20_000350"
     CDS             complement(914..1471)
                     /locus_tag="VCA20_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEP1834217.1"
                     /translation="MTNTRKMTIIAILSAVSFLLMYLKFPLIPTASFLEVDFSLVPIL
                     FGLLILDMKSSFAILLVRTFLKLILNNQGPSTIIGLPMNIAAMSVFILTVAYFWKNEQ
                     TIKNYLKAATIATLGSTAVMLVLNYVYAVPVYAAFANFDIKEILGLTNYLLMMVLPFN
                     LLQGIVLSAIFYICYKAAQPILKKV"
     regulatory      complement(1531..1745)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(1795..2340)
                     /locus_tag="VCA20_000351"
     CDS             complement(1795..2340)
                     /locus_tag="VCA20_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEP1834218.1"
                     /translation="MNIETLEYIEETAKNHIVLFEPQIPQNTGNIARTCAATNSPLHI
                     IKPMGFPIDDRKMKRAGLDYWDKLDVRFYDNLAEFMDYASKQGRVHLVSKFADKTYSD
                     EDYNDGNIHYFLFGREDKGLPEYFMREHPEKAIRIPMNDEHVRSLNVSNTACMIVYEA
                     LRQQQFSGLDLVHTYDGDKLK"
     gene            complement(2908..3147)
                     /gene="yidD"
                     /locus_tag="VCA20_000352"
     CDS             complement(2908..3147)
                     /gene="yidD"
                     /locus_tag="VCA20_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002272085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEP1834219.1"
                     /translation="MTRLLIGLVRFYQKFISPLFPPSCRYRPTCSAYMIETLQKHGLR
                     GFLMGLARIGRCHPFVEGGEDPVPDRFSLKRNKQK"
     gene            complement(3147..3878)
                     /locus_tag="VCA20_000353"
     CDS             complement(3147..3878)
                     /locus_tag="VCA20_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEP1834220.1"
                     /translation="MRINKYIAHAGVASRRKAEELIKQGLVTVNGQVVRELGTVIKSG
                     DKVEVEGQPIYNEEKVYYLLNKPRGVISSVSDDKGRKTVVDLLPTVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDEMIHPRNEINKVYLARVKGVANKEVLRPLTRGVVIDGKKTKP
                     AVYEIIKVDPVKNRSVVELTIHEGRNHQVKKMFEAVGLQVDKLSRTRFGNLDLIGLRP
                     GEYRKLNKKDISKLHTLAVTKPVKK"
     gene            complement(3868..4437)
                     /locus_tag="VCA20_000354"
     CDS             complement(3868..4437)
                     /locus_tag="VCA20_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HEP1834221.1"
                     /translation="MNRLAEIEVLLFVAGEDGLTLRNLAEMLEMQPTAVSQQLEKLSE
                     KYMADKASGLAILESSNRYKLVTKKEYANLLRIYAKTPINQTMSRALLETLSIVAYKQ
                     PITRIEVDDIRGVNSSGAISKLQAFDLIRENGKKEVLGRPNLYVTTDYFLDYMGINSL
                     EELPDVSEIDLIQEETELFVERNELENEN"
     gene            complement(4421..5140)
                     /locus_tag="VCA20_000355"
     CDS             complement(4421..5140)
                     /locus_tag="VCA20_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEP1834222.1"
                     /translation="MDIKLKDFEGPLDVLLHLVSKYQVDIYEVPITEVIEQYLAYIAT
                     LQAMRLEVAGEYMLMASQLMVIKSRRLLPKVVEQIDPEDDPEMDLLDQLEEYRKFKLL
                     SEKLGEQHDERANYFSKPKLDLIYDDVQLAKDKTVIDIFLAFSKVMAEKQASLRQSHA
                     TITRDEYKIEDMMDFVRSRFETGPRLELRQLFQESQDVNEMITIFLATLELVKVHEIV
                     LEQTETFGDIYLVRSEDESLS"
     gene            complement(5140..5871)
                     /gene="xerD"
                     /locus_tag="VCA20_000356"
     CDS             complement(5140..5871)
                     /gene="xerD"
                     /locus_tag="VCA20_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEP1834223.1"
                     /translation="MKQAIESFIQSKKVSVNSQKSYTYDLQQFVAVTKGEISQQSLLV
                     YQQSLLDLKPAAQKRKMSAVNQFLYFLYENNLLDRFYKLQTMSGPASVKKKLEREDLT
                     LLFQESPWLDGQLITLLIALLGLTPSEIAELTSQQVNLDFQVLTVEKGATKRVLTLPK
                     ELIPYMESHLSGRYVFDKKGQTYSRQWFFNRLTEFVQSIGKPDWTAQKLREQYILKQI
                     DEGKSLDQIAKQLGLKTSMSLEKFR"
     gene            complement(5868..6326)
                     /locus_tag="VCA20_000357"
     CDS             complement(5868..6326)
                     /locus_tag="VCA20_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEP1834224.1"
                     /translation="MIAREFEAFLLDQEETFLTPADKLAVIIDTHNIDHAKLLLSHMT
                     YSRVPVVTEDGRFFGTIGLTEIIKYQAENDLSDDELNRDISVIVKTDEGTVGLDYDLT
                     EVMRKLVDQSFLPVLGENREFMGIITRKSILKAINALLHNFPIASKEGKK"
     gene            complement(6323..6850)
                     /locus_tag="VCA20_000358"
     CDS             complement(6323..6850)
                     /locus_tag="VCA20_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEP1834225.1"
                     /translation="MAGASKTILVMSDSHGDRQIVEDIKHHYLGKVDAIFHNGDSELD
                     SQDRLWDGIQVVNGNCDYFGGYPDQLITQLDDVTIAQTHGHLYGINYGWQRLDYWAQE
                     VDADICLYGHLHVPDAEVRGKTLFLNPGSVSQPRGLVRECLYALVTIYDDHFQVDYYN
                     RQHQLYPALTKDISR"
     gene            complement(6823..7833)
                     /locus_tag="VCA20_000359"
     CDS             complement(6823..7833)
                     /locus_tag="VCA20_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HEP1834226.1"
                     /translation="MTDKIYEYRDEHNWFIGKASFANLFGSFGENGRVQEIYQIGQLF
                     DKLIAGNYEDENFNQCVKIEVIKLQSDFALFQFACDILNELNNRQFKVLQHQGAILVT
                     ENDKLLLVHLPQAGVSMADFFGQDKGLASVGDTILIATKNEGKTKEFRKFFERFGYQV
                     ENLNNYPDLPDVAETGMTFEENARLKAETIAELTGKMVLADDSGLKVDALGGLPGVWS
                     ARFSGPDATDELNNAKLLHELAMVFELKDRSAQFHCTLVMAAPNRDSLVVEADWEGFI
                     GMDLRGENGFGYDPLFLVGETGKTSAELTLEEKNKISHRAQALEKLVEAFPVWQEQAK
                     QS"
     gene            complement(7830..8624)
                     /gene="racE"
                     /locus_tag="VCA20_000360"
     CDS             complement(7830..8624)
                     /gene="racE"
                     /locus_tag="VCA20_000360"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEP1834227.1"
                     /translation="MDNRPIGFLDSGVGGLTVARELMRQLPHEEIVYIGDSARAPYGP
                     RPAEQIRDYTWQLVNFLLTKNVKMIVFACNTATAVAWEEVKEKLDIPVLGVILPGASA
                     AIKATQTGKVGVLGTAMTIQSDIYRQKIQALSPETQVASLACPKFAPLVESNSHQSSL
                     AKKVVYETLRPLVGKVDTLVLGCTHYPLLRPIIQNAMGKDVKLIDSGAECARDISVLL
                     NYFQINRSRTEKAIQHRFYTTASPAAFKEIAESWMGIDIHVEHVEL"
     gene            complement(8714..8950)
                     /locus_tag="VCA20_000361"
     CDS             complement(8714..8950)
                     /locus_tag="VCA20_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEP1834228.1"
                     /translation="MNLGLAILLIVLAFAGGVALGIYLSRKQVENYIADKPVLDENAL
                     RLMMSQMGQKPSEAKVQQVLRQIKSQQKVASKKK"
     gene            complement(9053..10300)
                     /locus_tag="VCA20_000362"
     CDS             complement(9053..10300)
                     /locus_tag="VCA20_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEP1834229.1"
                     /translation="MTKTPFVSKEVLETITEQFPTPFHLYDEKGIREKARALNAAFSW
                     NKGFKEYFAVKATPTPAILKILQEEGCGVDCATDVEVLMSEKLGFKDIMFTSNDTQAQ
                     EFVYARKVGATINLDAYEHIEFLKNVAGIPETVCLRYNPGGVFSLGTDIMDHPEESKF
                     GMTKEQLMKGYKELKELGVKEFGIHAFLASNTVTNDYYPVLARQLFELALEIREETGV
                     TLDFINLSGGIGVNYRPEQEPNDIAVIGEGVRKVYEEILTPAGMGHVKIFTELGRFML
                     APHGHLITKVLHRKETYRTYIGVDASAANLMRPAFYGAYHHITNITRPDAPIEVVDVA
                     GSLCENNDKFAVNRELPRAEVGDTLVIHDSGAHGFSMGYNYNGRLRSSEILLQEDGTA
                     RMIRRAETPEDYFATIYGFDFDR"
     gene            10434..11765
                     /gene="pepC"
                     /locus_tag="VCA20_000363"
     CDS             10434..11765
                     /gene="pepC"
                     /locus_tag="VCA20_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014607929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HEP1834230.1"
                     /translation="MSTLDFDFTERLYANYLANPSLQATENAVSHNGLLKSLETRQSA
                     IDNDYVFSIDLTKDAVSNQKASGRCWMFAALNTFRHKLISDFKLENFELSQAHTFFWD
                     KYEKSNWFLEQIIATADQEIGSRKVKFLLDTPQQDGGQWDMVVALFEKYGVVPKSVYP
                     ESISSSASRELNQYLNKLLRQDAQILRDLLAKGASSKEVQVQKENLLQEIFNFLAVNL
                     GLPPRSFDFAYRDKDNVYHRDTNVTPQAFYEKYVGLKLSDYVSIINAPTTDKPYNKSY
                     TVELLGNVVGAPAVRYLNVEMNRFKELAIAQLKAGESVWFGSDVGQSSNRQTGIMATN
                     TYDFSSGLGIHFHQDKAGRLDYSESLMTHAMVLTGVDLDDNEKPLKWKVENSWGDKVG
                     DKGYFVASDSWMDEYTYQIVVRKEFLTQEELAAYQAQPQVLAPWDPMGALA"
     gene            complement(11817..12518)
                     /locus_tag="VCA20_000364"
     CDS             complement(11817..12518)
                     /locus_tag="VCA20_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEP1834231.1"
                     /translation="MNNDSFIINQVDNTALNRFFGKIYGVVAMGIGLSALVSFLAVTV
                     FQSLLLSLLSAGSIIMMLIMIGQIALVVSASAMAAKNSPMALPMFLAYSVTNGITISM
                     ILMFYTSETVVLAFVSAALMFAIMAVIGMTTKKDLSGMAQALRAALWGIIIASVINIF
                     LRSSGLSFMMSIISVLVFSGLIAYDNQRIRNVFEQTGGNVGQGWVVSMALQLYLDFIN
                     LFLNLLRIFGGLSRD"
     gene            complement(12540..13022)
                     /locus_tag="VCA20_000365"
     CDS             complement(12540..13022)
                     /locus_tag="VCA20_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEP1834232.1"
                     /translation="MVAYKRDKEYMHYVGHLIATPKVQKLGKIPHHYYSTRLEHSINV
                     SYTSYKIAKKFGWDAKSTARGGLLHDLFFYDWRDTKFNKSHAWVHPRIAKRNAQKLIQ
                     LNKLEEDIIVKHMFGATIAPPRYKESWIVTCVDKYWAVREWSLPLQHKWKNRKVFRFQ
                     "
     gene            complement(13069..13809)
                     /locus_tag="VCA20_000366"
     CDS             complement(13069..13809)
                     /locus_tag="VCA20_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEP1834233.1"
                     /translation="MEIIRSKANHLVKQVKKLQQKKYRTSSYLIEGWHLLEEALAAKV
                     PIEHILVSEEHVHRVAGLSNVTVVSSDIMQDLADSRTPQGIVAQLSLPNQTLPDVLTG
                     KFLVLEDVQDPGNVGTMIRTADAAGFDGVFLSDKSADIYNMKVLRSMQGSHFHLPVYR
                     MPMTAIFSALKSNQLQILATTLSSQSVDYKEVTPNPSFALVMGNEGQGISTFVADEAD
                     QLIHITMPGQAESLNVAIAAGILLFSFI"
     gene            13940..14221
                     /locus_tag="VCA20_000367"
     CDS             13940..14221
                     /locus_tag="VCA20_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEP1834234.1"
                     /translation="MRKVKMIASGRVQGVGFRWSVQFLAVEIGDIYGRVWNNDDGTVT
                     ILAQSDNAEKLSHFIHEIRKGPSRMAKVTYLDVTLANFEDYKDFQVAYR"
     gene            14312..15205
                     /gene="yidC"
                     /locus_tag="VCA20_000368"
     CDS             14312..15205
                     /gene="yidC"
                     /locus_tag="VCA20_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006269468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEP1834235.1"
                     /translation="MLSGMALSTLLFLSGCVQTDKSGNPTGLIWDWLGQPMSNLIRFF
                     AEDQALGFGLAIIIVTLLVRFIILPLGIYQSWKAAIQSEKMNYLKPILGPIQERMKNA
                     SSQEEQLAAQQEYFATQKQYGVSMLGGIGCLPMLIQMPFFSAIYFAARHTPGISEATF
                     LGINLGSTSLILTVVAGILYYAQSLLMQVGMDEEQKKQMKTMALMNPIMIMMFSFGSP
                     AGVTLYWVVGGLFGILQQAITNFILKPRIRKQVEEEFKNITVSATPRKTKDVTPTASA
                     VIEGKTNNKKKNRNSGKQRSR"
     gene            complement(15289..15771)
                     /gene="greA"
                     /locus_tag="VCA20_000369"
     CDS             complement(15289..15771)
                     /gene="greA"
                     /locus_tag="VCA20_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEP1834236.1"
                     /translation="MAEKTYPMTLEEKEKLEKELEELKLVRRPEIVERIKIARSYGDL
                     SENSEYEAAKDEQAFVEGQISTIETKIRYAEIVNSDAVAADEVAIGKTVTVQEVGETE
                     EEVYHIVGAAGADAFANKISNESPIGHALIGKKTGDVATIETPAGSYDVKILKVEKTK
                     "
     gene            complement(15845..17662)
                     /gene="mltG"
                     /locus_tag="VCA20_000370"
     CDS             complement(15845..17662)
                     /gene="mltG"
                     /locus_tag="VCA20_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEP1834237.1"
                     /translation="MTKDTNEKNTQSSSFRDQILRDLEELKVKRLAEQSADGLVDKRN
                     EVINELQPSLAEEKAPHRETEIVKVEGIAFPDSYLSEEQQESEVMTESIEEPTSETLA
                     TPETFVIEKEVVSSPVPQDTVERNLEKLRNLVAANTAGFDEVPSSSSVSEPVESVLPG
                     NSPLEDTFLEFPTEDVSAVTGDTEIISLEETIIAPKTTAIVPEVDKQMQETQPRRRTT
                     HRTSKQRRKKQDNAAKHIVSVIMSIVVVAVLVTGVTGYMWVKSSLEPVNAKATETIQV
                     EIPEGSSTLEIGKILVDNKLIKNATIFNYYSKIKSYNNFQSGFYNLKQNMSVDDIAKA
                     LQESGTPTAQKEAAGKILIVEGYTLTQIAQAITDNTKTEDKNDKTPFTTEQFMATVTN
                     QDFINRMVATYPKLFASLPAADSGVIYQLEGYLFPAVYEYSDETTIEELVEQMIAAMD
                     NRLQPYYETIAAKNLTVNEVLTLASLVEKEGSTDEDRRNIASVFFNRLNAAMPLQSNI
                     AILYAQGKLGQETTLAEDAAIDTSIESPYNIYWTPGLMPGPVDSPSLSAIEAVINANT
                     TDYLYFVADVTTGNVYFTNNIDEHNQNVAKYVNAHLNNE"
     gene            complement(17734..18219)
                     /locus_tag="VCA20_000371"
     CDS             complement(17734..18219)
                     /locus_tag="VCA20_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEP1834238.1"
                     /translation="MEIRFATPSDLEQVVLIENANFSKEERIAESVLAIYLNALSKTC
                     LIMEHDGEIAGYLLSCPSVSQTVTDDIFYLTESDMPTGSHLAIASLSVADSYKGQGVG
                     TLLLAAIKEVALAGGFEGVSLTCKGYLIGYYEMNQFEDFGPSLSQFGGQMWSDMYWKA
                     L"
     gene            complement(18274..19605)
                     /locus_tag="VCA20_000372"
     CDS             complement(18274..19605)
                     /locus_tag="VCA20_000372"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEP1834239.1"
                     /translation="MTKTYHFIGIKGSGMSALALMLHQMGHKVQGSDVEKYYFTQRGL
                     EQAGIQILPFDEKNITADVELIAGNAFRPDNNVEIAYADAQGYTYKRYHEFLGEFMKG
                     FTSLGVAGAHGKTSTTGLLAHVMRNITDTSFLIGDGTGRGSANAQYFVFESDEYERHF
                     APYHPEYSIITNIDFDHPDYFTSLEDVFNAFNDYAKQVKNALFVFGEDEQLRRITANA
                     PIYYYGFEENNDFVAYDLRPSTSGSQFKVRHGEEELGEFQIPTFGKHNVMNATAVIAN
                     LYIAGFDLQLVAEHLKTFGGVKRRFTEKIVNDTVIIDDFAHHPTEIIATIDAARQKYP
                     SKELVAIFQPHTFTRTIALLDEFADALNGADAVYLAQIYGSARETDNGQVKVEDLAAK
                     INKKGGLVTVENTSPLLDHDNAVYVFMGAGDIQSYEYSFERLLSNLTNNVQ"
     gene            complement(19607..20269)
                     /locus_tag="VCA20_000373"
     CDS             complement(19607..20269)
                     /locus_tag="VCA20_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834240.1"
                     /translation="MIRHENRFPLVADDEVLVGENPIMSLYDESDLISNIRGPYQEKE
                     FSWSTDSQRVASAKPVAQTEDELLPPLFEAKPSHYSRKERLQQLTKTKPSPVKTQGQL
                     AREQAREDLKKKRSATYLRDEKPAPAKVVVKPPVVSTTESKAGRLTRLADNLRQTDYI
                     LADMPAVYSLKKEDREQEQVIKKNSYDFLRKSQVYNYPERRQQRDRQVAQELNLTHIE
                     EG"
     gene            complement(20348..23440)
                     /locus_tag="VCA20_000374"
     CDS             complement(20348..23440)
                     /locus_tag="VCA20_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEP1834241.1"
                     /translation="MGRMMPGRIRQEGIDLYEAGKLTVLHSENGKMVLDIAGERFTYG
                     DADSDLQCSCQLFQSKGYCQHLAATEYFLKNDASGKDMKQSLKKDGEQHKETVRRTYF
                     GGLFLDEILRPEPELGLKYQLSVEGSLLPYDRQIDWTLKITRLPDTRSYIVRDIGAFL
                     RIVKANGHYQIGKNYYEQVTYENFDEPSQALLDFLWALVPEKVGIDSDILTHFGRHFR
                     LPQAYFEEGLELLNQLEHFNFSYQQQSYSSLMVLPLTGEEGLYHFEVTVHTQMIEMVI
                     HEKTVRPLFHGRYLLVNGTVYSVNRHQEHLIYQISELIPTESGLRKVQVDFPDQNRLA
                     LSLLDLQTIGTVKAPKRFIIHDFKPEFHIQMEANESLSLQLVLDFDGRKVRSEEDLAL
                     LPFASHFQHLEKVYQAIRLAGFRGTYNAQRAALSQQELYPFFQQQLPILQKMGQVHLA
                     ERLQALYIEAKPQLEIVRNGSLLDISFDLSGIEQSEIDQAITALLNQEDHYTSPSGKV
                     FVFDEETKKISQTLIYLRARHSKEGQVQVHALAGYQLAQSLSQFNQVSFSKEFEEMAS
                     YLARPDLFPLPAVEVTTSLRDYQQTGLKWLTMLDTYGFGGILADDMGLGKTLQTIAFL
                     SSRMTKDSKVLILAPSSLIYNWLDECKRFAPSLDVAVVHGNKEQREEIIANGHQVLVT
                     SYPSFRQDVALYKQERFDYLILDEAQVMKNAQSKIAQLLREFEVGNCFALSGTPIENH
                     LTELWSIFQIVLPGLLPGKQDFGKLAAKDIARTIQPFVLRRHKEDVLQELPDLIEVNV
                     LNELTDEQKAIYLAQLQQMRTQIAGADDAQINRSKIEILSGITRLRQICDTPSLFMEE
                     FSGESGKLNSLKELLLQLKEGEHRVLIFSQFRNMLEKIEEQLVEMGMTSYTLTGSTPA
                     NQRQEMTQAFNAGSRDAFLISLKAGGVGLNLTGADTVILVDLWWNPAVEAQAISRAHR
                     MGQTEKVECYRLITRGTIEEKIQELQENKKNLVKTVLDGNESRANLTVEDILEILGIE
                     "
     gene            complement(23580..23888)
                     /locus_tag="VCA20_000375"
     CDS             complement(23580..23888)
                     /locus_tag="VCA20_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834242.1"
                     /translation="MEFFGELLVEFLTGLADFDEKKHPPFGIRYWLGWLGVLIHVLLL
                     ALLTCVTVFFFKFFLDGKGLINVVVTVVFLLFALFWLWKSGKTILKMWQATIYYLAIH
                     "
     gene            complement(23890..24228)
                     /locus_tag="VCA20_000376"
     CDS             complement(23890..24228)
                     /locus_tag="VCA20_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEP1834243.1"
                     /translation="MVAVPIWRSFLILLSFLSFFLVFYEDSQYKPFGLRYWLGVGACI
                     WLNFVTLASYFIAFTGGSIMVYNQYKQPLVVIFLFCLFVASMLTLLSFKAIKMMIRRT
                     KYYRRVRKEV"
     gene            complement(24296..25606)
                     /gene="der"
                     /locus_tag="VCA20_000377"
     CDS             complement(24296..25606)
                     /gene="der"
                     /locus_tag="VCA20_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEP1834244.1"
                     /translation="MALPTIAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRKFSLIDTGGIDDVDAPFMEQIKHQAEIAMDEADVIVFVVSGKEGVTDADEY
                     VSRILYKTNKPVILVVNKVDNPEMRNDIYDFYSLGLGDPYPVSSVHGIGTGDVLDAII
                     ENLPAQEVEENPDIIKFSLIGRPNVGKSSLINAILGEERVIASPVAGTTRDAIDTHFT
                     DPEGQEFTMIDTAGMRKSGKVYENTEKYSVMRAMRAIERSDVILMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTLEKDNHTMKQWEDDIRDQFQYLSYAPIIFVSALTKQ
                     RLHKLPETIKAISESQNTRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVATKP
                     PTFVVFVNEEELMHFSYMRFLENQIRKAFVFEGTPIHLIARKRK"
     gene            complement(25625..26338)
                     /locus_tag="VCA20_000378"
     CDS             complement(25625..26338)
                     /locus_tag="VCA20_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HEP1834245.1"
                     /translation="MNETIDLMLNHTSVRRFTEEPIEAEHLQAIISAGRAASSWKNFQ
                     SYSIIVVQSEEKKQAIYDLVPQPAILQAQAILVFVGDHNRASKAAQLHGSDFDAKGTE
                     NLLISSVDASLAGQNALLAAESLGYGGVFIGMIRHKALAIAELFNLPDYTYPIFCIAL
                     GRPAQNHPVKPRLASETIVFQEEYVDQGVEVIQAYDQVQTEYAGARQTETWSERMVAQ
                     FGQLEQPETKSILEKNKLL"
     gene            complement(26335..27234)
                     /gene="dnaI"
                     /locus_tag="VCA20_000379"
     CDS             complement(26335..27234)
                     /gene="dnaI"
                     /locus_tag="VCA20_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEP1834246.1"
                     /translation="MKSVQDRLSQATNPSPKSYQQLYQEIVSDPEVAAFIKKEGLTQQ
                     EITLSISKFLEYISQRDLFVKQDESYIAKGYQPVLVMNEGYADVSYLETEELVEYRRL
                     EAIKNRIQLINMPQALKKVTAEDLDFTDINRVEVYEAIADFVKRFEEKPKGLYIYGNF
                     GIGKSYLMAYLANLLSKTHLQSTTMLHYPTFVVDIKNAIKDGSVKERIDEIKMAQVLV
                     LDDIGAEQHSPWVRDDVLQVILQYRMQENLPTFFTSNFSFDDLERHFASGKSGDETWQ
                     AKRVMERIHYLARDLHLKGNNRR"
     gene            complement(27234..28385)
                     /locus_tag="VCA20_000380"
     CDS             complement(27234..28385)
                     /locus_tag="VCA20_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation/membrane attachment
                     protein"
                     /protein_id="HEP1834247.1"
                     /translation="MKPSDLFTYIKTSPFTPDIISLSQCYQPIISFDALALYYYLYSF
                     SDQGQVRYKWATILNHMDFGMKRLEQALDLLSAMELLQVYRADELIGLVLLPPLTVKQ
                     FLGKPLYKNLLAQRIGEASVESLQVSGPNKEKKVSKTFSQVFTIDGQVPVAFEPKHDF
                     DWSAFKALMAKDKLLFQDEAEDIIALSHIAEQAGWTWLETYRQAKATAIGQTISTKRL
                     QQSRQTLPTESANLTAHEQAIVREAKLKSSLTFLSFIKEQRKAAVTGTERKCLTDLAN
                     LGLLDEVINVLVLYTFNKVDSANLNEKYAMKLGNDFSYKGIGSAEAAVLYLRELKTGQ
                     VVTKSSQPQKSNVPEWSKEVVVMDNSPEAQARMEALRKRMLENESKGGS"
     gene            complement(28372..28863)
                     /gene="nrdR"
                     /locus_tag="VCA20_000381"
     CDS             complement(28372..28863)
                     /gene="nrdR"
                     /locus_tag="VCA20_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEP1834248.1"
                     /translation="MRCPKCQSLKSSVIDSRQAEDGNTIRRRRSCDQCGQRFTTYERI
                     EEKTLVVVKKDGTREQFSREKIFNGIIRSAQKRPVSTSDIDEVVNRIEQKVRSQSDGE
                     VESDVIGNFVMDELVELDEITYVRFASVYRSFKDVEELENLLKQMISKGSKVKSGAAH
                     EAK"
     gene            complement(28961..30085)
                     /locus_tag="VCA20_000382"
     CDS             complement(28961..30085)
                     /locus_tag="VCA20_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEP1834249.1"
                     /translation="MNTSFKEKVILILLSGATLLAVLNWSSIYGAIQQLLGSMNSLFI
                     GAIFAFILNVPMKKLEDQFEKNSFLKKSKRSLAIVGVLIGFALIVTGLVVIVLPTLVT
                     TVSELVSVSSTAIPKSVNALTNFLESNGLLSGQIGDQIASFLDQLKNLTLISSLVTPI
                     LSCLVSNVTGIFSNTMTLIMAFFFTLAILGSKEHLQAMTRKFLQATLPEKAVRVVNYI
                     GEVIVDTYDRFLMSQIVEACIIGTLVFVSYSLSGIPYASMAGILAGVLSFVPYIGPFS
                     ACLISALFVAVQNPLLALWSIALFQIIQLIEGNVIYPRVVGQSVGLPTLFTLAAALIG
                     GNLFGLLGMVFFTPIFAVIYRLVREWVTSRLNKKEDMTVS"
     gene            complement(30313..30702)
                     /locus_tag="VCA20_000383"
     CDS             complement(30313..30702)
                     /locus_tag="VCA20_000383"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834250.1"
                     /translation="MEAELEFYFKEVGYIDGNGVYQITNPNLLLEKAKSGDQNARKLY
                     QGYIASLMRTPYDFAKCVVSDYFGIFIDIANGRLLDALAKDLQSEAWNRAAMTILKLL
                     GKSTSKANVLATAAELAIAAYNCRGKR"
BASE COUNT         9092 a         6972 c         5814 g         9012 t
ORIGIN      
        1 cttcctcctt actcgccaga gtataatcct atcgagaaaa catgggctca tatcaaaaaa
       61 cacctcagaa gagtattgcc aaattgcgat acgtttcttg aggcactttc gtcctgctct
      121 tgtttcagtt tactataagc cacttctaca aaggacggaa aagcgaagta acgataatag
      181 aaagatgaca agttaaaact cctacctttt acagtaggag ttggtgtcaa attaactatt
      241 tttgcttact ttggaaacgc cattcaaagc gtttctgatc gtaaaatgga tacgtcattt
      301 gtaaaagagt gacggcaagt agccaaccac cgataatatc tgtcggatag tgaaccccaa
      361 tatagatacg ggatacagct accaggacag caagtagaat caatacggct tgtagcacaa
      421 gtctagctat gcctttcttc atcctctggt gaacaataat aacaactgct cctgcaatca
      481 tcatagttga tgcagtatgc cagctaggaa atgagtaacc gatggtatca atcaaccatt
      541 caatacttgg acgcggacgt tggtagacat atttcagggc tgtggatgcc acacccatcg
      601 ccgcaaaact agcaagtagg aaataagctt ctatcttcca ttttttatag tagtaaaaga
      661 tagcagctag aatcatggta atagctaaaa taataaccgt atttcctaaa acagtaatag
      721 aggtccaaaa ttgactggct ccacttggta aatttccacg aatgacagac tggacagtcg
      781 agtcaaaacc tgctagggta gtaggataaa attttactac atagcccaat atcacaaagg
      841 ctagagcaaa aaaagatgcg tttcgtaaat ggatttgttt attcttcata cagtataatt
      901 taccttataa aggttaaact ttctttaaaa taggttgggc tgctttgtaa cagatataaa
      961 atattgctga aagcacaatc ccttgtaaaa gattaaaagg aagtaccatc atcaaaagat
     1021 agttagtcag acccaagatt tctttaatat caaaatttgc aaaggctgca tagacaggca
     1081 ccgcataaac gtaatttaat accagcatca ctgctgtcga accaagcgtc gcaatagtcg
     1141 ctgcttttaa ataatttttt attgtttgtt catttttcca gaaataagct actgttaaaa
     1201 tgaaaactga catagctgca atattcatcg gtaagccaat aattgtcgat ggtccctgat
     1261 tatttaaaat cagtttgaga aaagttcgta ccaaaaggat agcaaaacta gacttcatat
     1321 ctagaataag cagaccaaat aaaatcggta cgagagaaaa atccacttcc aggaaactgg
     1381 ctgttggaat cagtggaaat ttcaaataca tgagcagaaa ggatactgct gagagaatgg
     1441 cgataatcgt cattttgcgt gtgtttgtca taaggttcct ccaatttttg aaaattgaag
     1501 aagcgtggaa gagcaggcaa aaggcctcca cttcgtcttc tcccatccag actttactgt
     1561 cggttgtgga attgcaccac atcagctttc gctcgcagac taccaacggt ctattttgtt
     1621 ctaatactgc gttagctcat ctcgacatac attcagtatg gtcttcgatt cgctgcctag
     1681 tcttaaaaca aattaccacg ttttacactg ccggtcggga atttcaccct gccctgaaga
     1741 ctatttcatc ataccaaaaa agccttgcat ttgcaagagc ttggtaagtt ttcactattt
     1801 taacttgtcc ccatcatatg tgtgaaccaa atctagacca gaaaactgtt gttgccgaag
     1861 agcctcatat acaatcatac acgctgtatt agagacatta agactacgca catgttcatc
     1921 attcattgga atacggatag ccttctctgg atgttcacgc ataaaatatt ctggcaagcc
     1981 cttatcttca cgaccaaata agaaataatg aatgttccca tcattatagt cttcatccga
     2041 ataggtttta tcagcaaatt tagacactaa gtgaacacgt ccttgtttgc tggcataatc
     2101 catgaattct gctaaattgt catagaagcg aacatctaac ttatcccaat aatctagtcc
     2161 tgcacgtttc atcttacggt catcaattgg aaatcccata ggcttaatga tatggagagg
     2221 actattggtt gcagcacaag ttcgagcaat attgcctgta ttctgtggaa tctgtggttc
     2281 aaataagaca atgtggtttt tggctgtttc ttctatatac tctaatgttt caatattcat
     2341 aaggacctct ctggtgacat ttcactaaac acgaataccc ttcgaaaatc aaatccaaac
     2401 catgtcagct acattttacc gtataccagt acagtctgct gcttgttgtc tagtttcttt
     2461 ttttgatttt cattgagtaa aaagactcga ctgtctccta tggcaagaaa gacaaaaaaa
     2521 tagccacact gcccggagtc aagctcagca aacagcatgg tgaatgatga ttcattcact
     2581 taactcacaa caggtttgag ttaaatcaat gaaatcgtga tactattgta gcttaaagaa
     2641 ggctgaaaat caagagtttt gccccatttt tttcaaattt tcatagaaaa tcatggaggc
     2701 tgggcaaaaa gaccaacctc gcttctcaga gttcgtgtca acatctgagc gcagtgatta
     2761 attggcttta acagtccagt ggactgttaa tattgaggcg aacttgcttc gccctatctc
     2821 cagccttcaa aggctcccga accttttgat agagtactaa aggcaaccta aaaacctata
     2881 tttatacaga acaaaaagta ggttgcccta cttctgcttg tttcttttca aactaaacct
     2941 gtccggcaca ggatcttccc caccttccac aaagggatga cagcgaccaa ttcgagccaa
     3001 ccccatcaaa aagcccctga gtccatgttt ttgtaaggtc tcaatcatat aagccgaaca
     3061 agttggacgg tagcgacaag agggcggaaa aaggggagaa ataaatttct gatagaatcg
     3121 gaccaagccg atcaaaagtc tagtcattat ttttttactg gcttagtaac agccagggta
     3181 tgtagtttac tgatgtcttt cttattgagc ttgcggtatt ctcctggacg taggccaatc
     3241 aagtcaagat taccaaagcg agtacgagac aacttatcta cctgcaagcc gacagcctca
     3301 aacatcttct taacctggtg gtttcgacct tcatggattg ttaattccac aactgagcgg
     3361 ttcttaacag ggtccacctt aataatctcg tacaccgcag gcttagtctt tttgccatca
     3421 atgacaacac cacgggtcaa gggacgcaaa acttccttgt tggcaacccc tttgacacgc
     3481 gccaagtaaa ccttattaat ctcattacgt gggtgaatca tttcgtctgt aaaatcaccg
     3541 tcattggtca aaatcaagac cccagatgta tcccagtcca aacgaccaac aggataaatc
     3601 cgctccttaa ctgttggcaa caagtccaca actgtctttc ttcccttgtc gtctgacaca
     3661 ctagaaataa cacctcgtgg tttattgagg agatagtaga ccttctcttc gttataaatt
     3721 ggttgtccct caacctctac cttgtcaccc gatttgataa ctgtccctag ctcacgaaca
     3781 acctgaccat ttacagtaac tagtccttgt ttgattagtt cttctgcctt acgacgactg
     3841 gcaactccag catgggcaat gtatttatta attctcattt tctaactcat ttctttctac
     3901 gaatagctct gtttcttctt gaatcaaatc tatttcagac acatcaggca attcttctag
     3961 actattaatt cccatgtaat ccaggaaata atccgttgtc acatagagat ttggtctacc
     4021 aagaacctct ttcttaccgt tctcacgaat gaggtcaaag gcctgtaatt tactgatggc
     4081 accgctggaa ttgactccgc gaatatcatc aacttcaatc cgagtaatgg gttgcttata
     4141 ggctacgatt gataaggttt ccaaaagagc ccgtgacata gtctgattga ttggtgtttt
     4201 ggcatagatt cgcaaaaggt ttgcatattc ttttttagta accaacttgt agcgattgga
     4261 agactctaag atggctaagc ctgacgcttt gtctgccata tatttctcac taagtttctc
     4321 taactgctgg ctgacagctg tcggctgcat ttccaacatc tctgccaaat ttcgtaacgt
     4381 caagccatcc tcacctgcca caaagagcag gacttcgatt tcagctaagc gattcatctt
     4441 cactccttac tagataaata tctccaaaag tctctgtctg ctccaagacg atttcatgaa
     4501 ccttgaccaa ttccaaagtc gcaaggaaga tcgtaatcat ttcattgaca tcttgacttt
     4561 cttgaaaaag ctgacggagt tcaaggcgtg gaccagtctc aaaacgactg cgaacaaagt
     4621 ccatcatgtc ttcgatttta tattcatcac gggtaatggt cgcatgagac tgacgaaggc
     4681 ttgcctgttt ttcagccatt actttagaaa aagctaggaa aatgtcaatg acggtcttat
     4741 ccttggctaa ctgtacatca tcataaatca aatctaattt gggttttgaa aaatagttcg
     4801 cccgttcatc atgttgctct cctaatttct cactgagaag cttgaacttg cggtattctt
     4861 ccaactggtc caagaggtcc atttcaggat catcttcagg atcaatttgc tcaacaacct
     4921 tcggcaataa ccgacgactt ttgatgacca tcaattgact agccatgagc atatattctc
     4981 ccgctacctc taaacgcatg gcttgcaagg tagcaatgta ggccaaatat tgctcaataa
     5041 cctctgtaat cggaacctcg taaatatcta cttgatactt ggacaccaga tgtagcagga
     5101 catcaagtgg cccttcaaaa tcttttaatt ttatatccat tatctaaatt tttccaaact
     5161 catgcttgtt ttcagtccca actgcttggc gatttggtcc aaggattttc cttcgtctat
     5221 ctgttttaga atatactgct cccgtaattt ttgggctgtc caatcaggct tgccgatgga
     5281 ctggacaaat tctgtcagac gattaaaaaa ccattgacga gaataggtct gtcccttctt
     5341 atcgaagaca tagcgtccag acagatgaga ctccatatag ggtatcaatt ccttgggcag
     5401 agtgagcacc cgcttggttg ctcccttctc aactgtcagg acttgaaaat ccagattgac
     5461 ctgctgacta gtcagctctg ctatttcact aggagtcagc cctagtaaag caatcaaaag
     5521 agtaatcagt tgcccatcaa gccaaggact ttcttgaaag aggagagtta aatcctcacg
     5581 ctccagtttc tttttgacgc tggctggacc tgacatcgtt tgcaatttat aaaaacgatc
     5641 caggagatta ttttcataaa gaaagtagag aaattgattg accgctgaca tttttctttt
     5701 ctgagcagcc ggtttcaagt ctagaagaga ctgctgatag accaaaagag actgttggct
     5761 aatctccccc tttgttactg ccacaaactg ctgcaggtca taggtatagg acttttggct
     5821 gttgacactg acctttttgc tctggataaa agattcgatc gcctgcttca tttcttgcct
     5881 tcttttgatg ctatggggaa attatgcaag agagcattga ttgcctttaa gatggatttt
     5941 cgtgtaatga ttcccataaa ttctctattt tctccaagga cgggtaagaa agattgatct
     6001 actagcttgc gcatcacttc tgtcaaatca taatccagac caacagtccc ttcatctgtc
     6061 tttacaatca cagaaatatc tctgtttaac tcatcatccg ataggtcatt ttcagcttga
     6121 tatttaatga tttccgtcaa gccgatggtt ccaaaaaagc ggccatcttc tgttaccaca
     6181 ggtacgcgtg aataggtcat gtgactgagt aacagttttg catggtcaat attatgcgta
     6241 tcaataatta ctgccaattt atccgctggt gtcaagaaag tttcttcctg gtccaataaa
     6301 aaggcttcaa attctcttgc aatcatcttg aaatatcctt tgtcaatgct gggtaaagtt
     6361 ggtgctgtcg attgtagtaa tccacctgga agtgatcatc atagatggtc acaagagcat
     6421 agagacattc tctgaccaaa ccacgaggtt gactaacgga gccaggattg aggaagaggg
     6481 tcttgccacg cacttcggca tcaggcacat gcagatgccc atacaaacaa atatccgcat
     6541 caacttcctg tgcccaataa tccaaccgct gccagccgta attaatgcca taaagatgac
     6601 catgagtctg agcgatagtc acatcatcca attgagtaat gagctggtca gggtaaccac
     6661 caaaatagtc gcagttccca ttgaccactt gaatgccatc ccacaaacga tcttgactat
     6721 ccagctccga atcaccattg tgaaagatgg catcaacctt gccaagataa tgatgtttaa
     6781 tatcctctac aatctgtcta tccccatgag agtcgctcat gactaggatt gttttgcttg
     6841 ctcctgccat actggaaatg cctccactaa tttttctagt gcttgtgcac ggtgggaaat
     6901 cttatttttt tcttctaagg ttaattctgc tgaggtctta cctgtttcgc ctacgaggaa
     6961 aagtggatca tatccaaacc cattttcccc acggaggtcc ataccgataa agccttccca
     7021 gtccgcttcg acaaccaaac tatcacgatt tggagcagcc atgaccagcg tacaatggaa
     7081 ttgtgccgaa cgatctttca gttcaaatac catggccaat tcgtgcaaga gcttggcatt
     7141 attgagctca tctgtggcat ctgggcctga aaaacgggca gaccaaactc ctggcaaacc
     7201 acctaaagca tcaaccttca aaccagaatc atctgccaag accatcttgc ctgtcagctc
     7261 tgcaatggtt tcagccttaa gccgtgcatt ttcctcaaaa gtcatcccag tttcagccac
     7321 atctggcagg tcaggatagt tgttgaggtt ctcaacttgg tagccaaatc gttcaaaaaa
     7381 cttacggaat tccttagtct taccttcatt tttggtggca atcaagatgg tatctcccac
     7441 gcttgccaat cccttgtctt gaccgaagaa atctgccatg ctaacccctg cttggggcag
     7501 atggactaag agaagtttgt cgttctctgt taccagaata gctccttgat gttggagaac
     7561 tttgaattgg cgattgttta actcattcaa aatatcgcag gcaaattgga agagggcaaa
     7621 gtccgactga agtttgatca cttcaatctt gacacactgg ttaaagttct catcttcata
     7681 attaccagca attagtttgt caaataattg accaatctgg taaatttcct gcactctgcc
     7741 attttcacca aatgaaccaa atagattggc aaagctggct tttccgataa accaattgtg
     7801 ttcatctctg tattcgtata tcttgtctgt catagttcta catgctccac atgaatatca
     7861 atgcccatcc agctttcagc tatttcttta aaagctgccg gactagctgt tgtgtaaaat
     7921 ctgtgctgaa tagccttttc ggtacggctg cggttgattt gaaaataatt taacaaaaca
     7981 gaaatatctc gcgcgcactc tgccccactg tcaatcaact tgacatcctt gcccatggca
     8041 ttctggatga tggggcgaag taggggataa tgggtgcagc ccaaaaccaa ggtgtccact
     8101 ttcccaacga gtggccgaag tgtctcgtaa acaacctttt tggcaagact ggactggtgg
     8161 ctattggact caaccagtgg tgcaaatttg ggacaggcca gactggctac ctgcgtttct
     8221 ggagaaaggg cctgaatctt ttgtcgataa atatctgatt gaatggtcat agccgttcct
     8281 aagacaccaa ccttaccagt ttgcgttgcc ttaatagctg ccgatgcacc tggtaaaata
     8341 acacccaaaa caggaatatc taacttttcc ttgacttctt cccaggctac cgctgttgcc
     8401 gtattgcagg caaagacaat catttttacg ttttttgtca aaaggaaatt gaccaactgc
     8461 caagtataat ctctgatttg ttccgctgga cggggaccat aaggtgcccg tgccgagtcc
     8521 cctatataaa cgatttcttc atggggaagc tggcgcatta actcacgcgc aaccgttaat
     8581 cccccaaccc ctgaatctaa aaaacctatt ggtcgattat ccataatact gagaatagcc
     8641 agaaaatctg actagccttt cttttctact caaaaaatgg gatctgggaa tgaatatccc
     8701 agtcccagtt tgattatttt ttcttgcttg ccactttttg ttggctcttg atttggcgaa
     8761 gtacttgttg tacttttgcc tcgcttggtt tttgccccat ttggctcatc atcaaacgca
     8821 aagcattttc atccaaaact ggcttgtcag caatgtagtt ttcaacttgt ttgcgtgaca
     8881 agtaaatacc caaggctaca ccacctgcaa atgccaatac aataagtaaa attgctaaac
     8941 ctaagttcat ttgtcaatct cctgactctt ctaaaaataa ttcattcgga attgccctgc
     9001 gacaatctct taatgaatct attataccaa atttagcagg cttttccaag acttacctgt
     9061 caaaatcaaa accgtaaatg gttgcgaaat aatcctcagg tgtttcagca cgacgaatca
     9121 tacgcgctgt gccatcttcc tgcaaaagga tttcagaaga acgcagacga ccgttgtagt
     9181 tgtagcccat ggagaagccg tgggcaccac tgtcatgaat gaccaaggtg tctcctactt
     9241 ctgcccgtgg taattcacga ttaaccgcaa acttgtcgtt gttttcacaa agggaacctg
     9301 ccacatccac cacttcgatt ggcgcatctg ggcgcgtgat attggtgata tggtggtagg
     9361 cgccatagaa ggcaggacgc atgaggttgg ctgccgatgc atcgacaccg atataagtcc
     9421 gataggtttc cttacgatgg agcacctttg taatcaagtg accgtgtggc gccaacataa
     9481 agcgacccaa ttccgtaaaa attttgacat gtcccatacc agctggcgtg agaatttcct
     9541 cataaacctt acggacaccc tcaccaatga cagcaatgtc gtttggttct tgctcaggac
     9601 gataattgac tccaatccca cctgatagat tgatgaagtc caaggtcaca cctgtttcct
     9661 cacgaatttc caaggccaat tcaaaaagtt gacgagctaa gacaggatag tagtcatttg
     9721 ttactgtatt cgaagctaga aaggcatgaa taccaaattc cttgacacca agctctttta
     9781 gttccttata gcctttcatc aactgttcct tggtcatccc aaacttagac tcttctgggt
     9841 ggtccataat atccgttcca agcgagaaga ctccacctgg attgtaacgg aggcagaccg
     9901 tctctgggat accagcaaca tttttcaaaa actcaatatg ctcgtaggca tcaagattaa
     9961 tggtcgcccc aactttacga gcatagacaa attcttgcgc ctgggtgtca ttagatgtga
    10021 acatgatgtc cttaaagccc aatttttcac tcatcagcac ctctacatct gtcgcacagt
    10081 caactccaca accttcttct tgcaagattt tcaaaatggc tggcgtcggt gttgccttga
    10141 ccgcaaaata ttccttaaag cctttgttcc atgaaaaagc tgcattaagg gcacgggcct
    10201 tctcacgaat acctttttca tcatacaaat gaaaaggagt cggaaattgc tccgtaatgg
    10261 tctctaaaac ttctttacta acaaatggtg tcttggtcat ctcgctacct catcaaatat
    10321 ttttcctatt atatcacaaa tatgtgagga actgtagctt tcaactccac caaatacttg
    10381 caaaataaat ttctagcgct ataatgttat tatcattaaa aaaggagtaa tatatgtcaa
    10441 cattagattt tgattttaca gaacgcttgt atgccaacta tctagctaat cccagcctac
    10501 aggcaaccga aaatgccgtt agccacaacg gacttttgaa atccttagaa acacgccaaa
    10561 gcgctatcga caatgactat gtcttttcaa ttgacttgac caaggacgct gtttcaaacc
    10621 aaaaggcttc tggacgttgc tggatgtttg ctgcattaaa caccttccgc cacaagctca
    10681 tctctgactt caaactggaa aattttgagc tatcacaggc ccataccttc ttttgggata
    10741 aatatgaaaa gtccaactgg ttcctcgaac aaatcattgc cacagctgac caagaaattg
    10801 gtagccgtaa ggtaaaattc ttattggata ctcctcaaca agatggcggt caatgggata
    10861 tggttgttgc tttatttgaa aaatatggtg tggtaccgaa atccgtctat ccagaatcta
    10921 tctcatccag tgccagccgt gagctcaatc agtaccttaa caaattgctc cgtcaagatg
    10981 cacaaatctt gcgagacctc cttgccaaag gagcttcctc gaaagaagtt caagtacaaa
    11041 aagaaaactt acttcaagaa atctttaact tcctagccgt caaccttgga ctccctccac
    11101 gcagctttga ttttgcatat agagataaag acaatgtcta tcaccgcgat acaaatgtga
    11161 cacctcaggc attctacgag aaatacgttg gtttgaaact atctgactat gtctccatta
    11221 tcaatgctcc tacaactgac aaaccttaca ataaatctta tacagtcgaa ttgttgggga
    11281 acgttgttgg cgcccctgct gtccgctatc tcaatgtaga aatgaaccgt tttaaagaac
    11341 tggctattgc ccaactcaaa gctggtgagt ccgtttggtt tggttccgat gtcggtcaaa
    11401 gtagcaaccg tcaaactggt attatggcaa ccaacaccta cgacttctct tcaggtctgg
    11461 gtatccattt ccatcaagac aaggctggaa gattagacta ctccgagagc ttgatgaccc
    11521 acgctatggt cttaactggt gttgatttag acgataatga gaaacctctc aaatggaaag
    11581 tagaaaattc ttggggtgac aaggttggtg acaagggata cttcgtcgca tcagatagct
    11641 ggatggacga gtacacttat cagattgttg tccgaaaaga attcctcaca caagaagaac
    11701 tagcagctta tcaagctcaa ccacaagtcc ttgccccatg ggatccaatg ggagctctag
    11761 cataacaaac gacatataaa aaaagagggt aatcctcttt tttatatgtg tcataattag
    11821 tctctactca aaccaccaaa gatacggagt aggttgagga agaggttgat gaagtcaaga
    11881 tagagctgaa gcgccatgga tacgacccaa ccttggccaa cattaccacc agtttgttcg
    11941 aaaacattgc ggatacgttg gttgtcataa gcaatcaaac ctgagaatac caaaacagaa
    12001 atgatagaca tcataaagct tagaccagag ctacgaagga agatattaat aacacttgca
    12061 ataataattc cccatagagc tgcacgcaat gcctgagcca taccagaaag atccttcttc
    12121 gttgtcatcc caattacagc catgatagca aacataagag ccgctgacac aaaggcaaga
    12181 acaactgttt cgctggtata gaacatcaag atcatgctga tggtaatccc atttgttact
    12241 gagtaggcaa gaaacattgg tagagccatt gggctatttt tagctgccat tgctgaagca
    12301 gaaacaacca aggcaatctg accaatcata atcagcatca tgatgatgga acctgcactc
    12361 agcagactaa gcaataaaga ttgaaagacg gtcacagcca agaaagaaac tagagctgac
    12421 agaccaattc ccatggcaac cacaccataa attttaccga agaaacggtt gagggcggta
    12481 ttatccacct gattaataat aaatgaatcg ttattcatac tattctcctt taaatcatct
    12541 tattgaaaac ggaagacctt acgatttttc cacttgtgtt gtaagggcag actccattct
    12601 ctcacagccc aatacttatc cacacatgtc acaatccagg attccttgta gcgaggagga
    12661 gcgattgtcg caccaaacat atgcttcaca atgatatctt cttctagttt attgagttga
    12721 atgagtttct gagcattgcg tttagcaatt cgtggatgaa cccaagcatg gctcttgttg
    12781 aatttggtat ctctccagtc atagaagaat aaatcatgca atagaccacc acgagctgtt
    12841 gacttggcat cccaaccaaa ttttttcgca atcttatagc ttgtataaga gacattgatg
    12901 gaatgttcca agcgcgtgga gtaataatga tgaggaatct tccccaattt ttgcaccttg
    12961 ggtgtggcaa tcaagtgccc cacgtaatgc atgtattctt tatctcgttt atatgcaacc
    13021 attcgttaac ctcgttggaa tattatacca cactatagta cacttggctt aaataaagct
    13081 aaacaataga atacccgccg caatggctac attgaggctt tccgcctgcc ctggcatggt
    13141 aatatggata agttgatccg cctcatcggc tacaaaagtt gaaattcctt gaccttcatt
    13201 tcccatgacc aaggcaaaac tcggatttgg cgtaacttcc ttatagtcaa ccgactgact
    13261 agagagggtt gttgccaaga tttgtagctg attgcttttc aaagcagaaa aaatagcagt
    13321 catcggcatc cgataaactg gcaagtggaa atgactccct tgcatggaac gcagaacttt
    13381 catattgtaa atatctgccg acttatccga tagaaatact ccatcaaaac cagctgcatc
    13441 agctgtacga atcatggtac caacatttcc aggatcttgt acatcttcca gaaccaaaaa
    13501 ttttcctgtt aagacatcgg gtaaggtctg atttggtaag gacagttgag ctactatccc
    13561 ttgcggtgtc cgtgagtctg ctaaatcctg cataatatct gaactgacaa ctgttacatt
    13621 ggataagcca gcaacccgat gaacatgttc ttctgatacc aagatatgtt caataggtac
    13681 tttggctgcc aaagcctcct ccaacaaatg ccaaccttca atcaaataag aagaagtacg
    13741 gtattttttc tgttgtaatt tcttaacctg tttgaccaaa tgattggcct ttgagcgaat
    13801 gatctccatg aatgcccctt tttcttttac tgctcagcaa cttcaatttt ccaacggcta
    13861 aatcaattat cttgattttt gaacagtctt acccaaccat gatataataa agtaaatgtc
    13921 ttgtcaaaag gagaaactta tgcgaaaggt aaaaatgatt gcatctggtc gtgtgcaagg
    13981 agtcggcttt cgttggtctg ttcaattttt agctgtggaa ataggcgaca tctacggcag
    14041 agtttggaat aatgatgatg gaactgtcac cattttagct cagtcagata atgctgagaa
    14101 gctcagccat tttatccatg aaattcggaa agggccttct cgtatggcca aagtcaccta
    14161 tctagatgtc accctagcca acttcgaaga ctataaggat tttcaggtgg cgtatagata
    14221 aaacttgtat attttgaaca aaaaaagtaa aatagagagt atactgaaaa aaacaaagga
    14281 acgaaaaaaa ttgaaaaata aacgtctatt catgttgtca ggcatggcct tatcaacact
    14341 tcttttctta tctggttgtg ttcaaactga taaaagcgga aatcctacag gtctcatttg
    14401 ggattggttg ggacaaccaa tgtccaactt gattcgcttc tttgcagaag atcaagctct
    14461 aggatttggt cttgctatta tcatcgtcac cttattggtt cgcttcatca tcctcccatt
    14521 gggaatttac caatcttgga aagctgctat ccaatctgag aagatgaatt atctcaaacc
    14581 gattttaggc ccaatccagg agcgcatgaa aaatgcaagc tctcaagaag agcaacttgc
    14641 tgcgcaacaa gagtattttg caactcaaaa acaatacggt gtgagcatgc tcggcggaat
    14701 tggttgtttg ccgatgctga ttcaaatgcc attcttctct gcaatctact ttgcggctcg
    14761 ccatacacca ggaatttcag aagctacttt ccttggtatt aatttaggtt caactagcct
    14821 gattttgaca gttgttgctg gtattttata ctatgcccaa tcgctcttaa tgcaagtcgg
    14881 catggatgaa gaacagaaaa aacaaatgaa aaccatggct ctgatgaatc caattatgat
    14941 catgatgttc tcatttgggt ctcctgctgg agtgactctc tactgggttg tcggtggtct
    15001 cttcggtatt cttcaacaag ctattacaaa cttcattttg aaacctcgta ttcgtaaaca
    15061 agtggaagag gaattcaaaa atatcacagt gtcagcaact cctcgtaaaa caaaggatgt
    15121 tactccaact gcttcagcag tcattgaagg aaaaacaaac aataagaaga aaaatcgtaa
    15181 ttctggaaaa caacgttcta gataataaac actaaaagat tgggatcccc aatctttttt
    15241 agtaccttcg tgaataaaaa gagagaatct ttcgactctc tccacacctt atttcgtttt
    15301 ttcaaccttc aagattttta catcatagct accagcaggt gtttcaattg ttgcaacatc
    15361 tcctgttttt ttaccaatca aagcatggcc aattgggctt tcatttgaaa tcttgtttgc
    15421 aaaggcatct gcacctgcag caccaacgat atgatagact tcttcctcgg tctcaccaac
    15481 ttcttgaaca gtcacagtct taccaattgc cacttcatcc gcagcaactg catcactgtt
    15541 tacgatttca gcatagcgaa tttttgtttc gattgttgag atttgacctt caacaaaagc
    15601 ctgttcatct ttagctgctt cgtactcaga gttttctgaa aggtctccgt aagaacgagc
    15661 aattttaata cgttcaacaa tttctggtcg acgaactagc tttagttcct ctaattcttt
    15721 ttctagtttt tccttttctt ccaaggtcat tggatatgtt ttttctgcca tttgtctttc
    15781 cttttctttt atttttcatg aaaacaaaat tatgtggaaa accacataat tttgttgtaa
    15841 gaatttactc attattaaga tgtgcattta catacttagc aacgttttgg ttatgttcat
    15901 caatattatt tgtaaagtaa acatttccag ttgtaacatc tgctacaaag taaagatagt
    15961 ctgtcgtgtt ggcattaatc actgcctcga tagccgaaag gcttggacta tccactggtc
    16021 caggcatcaa tcctggtgtc caataaatgt tgtatggtga ctcaattgat gtatcaatcg
    16081 ctgcatcttc tgctagagtt gtttcttgac caagtttacc ctgagcatac aagattgcaa
    16141 tattggattg tagaggcatt gctgcattca agcggttaaa gaagacactt gcaatgtttc
    16201 gacgatcttc atcagtagat ccttcttttt caaccaaaga tgccaaggta agaacttcat
    16261 ttacagtcaa attcttagca gcaatcgttt cataatatgg ttgtaaacga ttatccatcg
    16321 cggcaatcat ttgctcaacg agctcttcga tagttgtttc atcgctgtat tcataaaccg
    16381 ctgggaagag gtaaccttcc aactgataaa taactccact atcagcagct ggtaagctgg
    16441 caaacaattt tggataagta gcaaccattc gattaataaa atcttggttg gtcacagtag
    16501 ccataaactg ttctgtggta aatggtgttt tatcattttt atcttctgtc ttggtattat
    16561 ctgtaatagc ctgagcaatt tgtgtcagtg tatacccttc aacaatcaaa atcttacctg
    16621 cggcttcttt ttgagctgtc ggagtgccac tttcttgaag tgctttggcg atgtcatcca
    16681 cagacatatt ttgttttaaa ttataaaaac cactttggaa attattataa ctcttaattt
    16741 tagagtagta attgaaaata gtagcattct taatcaactt gttgtcaact aaaattttac
    16801 caatttctaa agttgaagat ccttctggaa tctctacctg aatagtttct gtagcttttg
    16861 cattaactgg ttccagacta gattttaccc acatatagcc agtaacacct gtcactagga
    16921 ctgccactac aacaatcgac atgataacag agacaatgtg tttagcggcg ttatcttgtt
    16981 ttttacgacg ttgcttgctt gttctgtggg ttgttcttct tctgggttgt gtttcctgca
    17041 tctgcttgtc tacttctgga acaatagccg ttgtctttgg agcaataata gtttcctcta
    17101 gcgagatgat ttctgtatca ccagtcacag ctgacacgtc ttccgttgga aattccaaaa
    17161 atgtatcttc taaaggagaa tttcccggaa ggacagactc tactggctca gaaactgacg
    17221 agcttgatgg tacttcatca aaccctgccg tattagcagc cacaagattc cttaatttct
    17281 ccaagtttcg ttcaacagta tcttgtggaa ctggacttga aacaacttct ttttcaatca
    17341 caaaagtctc tggagtagcc aaagtttctg atgttggctc ttcaatactt tccgtcatca
    17401 cttctgattc ttgctgttct tctgacaagt atgaatcagg gaaagcaata ccttctactt
    17461 ttacaatttc agtctctcta tggggggctt tttcttcggc aagactaggc tgaagctcgt
    17521 tgataacttc attacgctta tcaacaagac catccgctga ttgttccgcc aaacgtttca
    17581 ccttcaattc ttccaaatct cgtaaaattt gatcgcggaa gcttgaagac tgcgtgtttt
    17641 tttcattcgt atcctttgtc acgctgttac tcctttcaca cagttactat acattatatc
    17701 ttaaaggctt atgaattgca agaacttccc tttctacaag gctttccaat acatatcact
    17761 ccacatttgt cctccaaatt gcgacaagga ggggccaaag tcttcaaact ggttcatttc
    17821 atagtagcca atcaaatacc ccttacaggt cagggaaact ccttcaaatc ctcccgctaa
    17881 agccacttct tttatggctg ccaacaaaag agttcccact ccctgtccct tataggaatc
    17941 cgctacggat agactggcaa tagctaggtg actgcctgtc ggcatgtcac tctctgtcaa
    18001 gtaaaaaata tcgtctgtaa cagtctgcga aacagaggga caggataata aatatcctgc
    18061 tatttcccca tcgtgttcca ttattaaaca ggttttactc aaagcattca gataaatggc
    18121 aagaacagac tcagctatcc gttcctcttt agaaaaattg gcattttcaa tcaaaacaac
    18181 ttgttctaaa tccgaaggag tagcaaaacg aatttccata attttctccc ttatatagcg
    18241 gaaaaacagg ggcataggcc tccgtctttc tttttattgg acgttatttg taagatttga
    18301 caagagtctt tcgaaggaat attcatagga ctgaatatca ccagctccca taaatacata
    18361 tactgcattg tcatggtcta aaagtggtga agtgttttca actgtaacaa gtccaccttt
    18421 tttgttgatt ttagccgcca aatcttctac cttaacctga ccgttgtctg tttcacgcgc
    18481 tgagccataa atttgagcaa ggtaaactgc atctgctcca ttcaaagcat ctgcaaactc
    18541 atcgagaagc gcaatggtac gagtgaatgt atgtggttgg aagatggcta ccaactcctt
    18601 gctaggatac ttctgacgtg ccgcatcaat ggttgcaatg atttccgttg gatggtgagc
    18661 aaagtcatca ataataacag tatcattgac aattttctcc gtaaagcgac gtttgacacc
    18721 tccaaatgtc ttcaagtgtt ctgcaaccag ttgcaaatca aatccagcaa tatagagatt
    18781 ggcaataaca gctgttgcat tcatcacatt atgcttacca aaggttggaa tttggaattc
    18841 tcccaactct tcttcaccat ggcgaacttt gaattgtgaa ccacttgtag aaggcctcaa
    18901 gtcataggca acaaaatcgt tgttttcctc aaaaccatag taataaattg gagcatttgc
    18961 cgtgatacga cgaagttgct cgtcttcacc aaaaacgaag agagcatttt ttacttgctt
    19021 cgcataatca ttgaaagcat tgaaaacatc ctctagactg gtgaaataat ctggatggtc
    19081 aaaatcaata ttggtaatga tgctgtattc aggatggtat ggggcaaagt gacgttcgta
    19141 ttcatcggat tcgaacacaa aatactgagc atttgccgaa ccgcgaccag taccatcacc
    19201 aatcaagaag gaagtatctg tgatattgcg cataacatgt gccaataatc ccgttgtaga
    19261 ggtcttacca tgggcaccag caacaccgag gctcgtaaaa cctttcataa attcacctag
    19321 aaattcatgg taacgtttgt aggtatagcc ctgggcatca gcataagcga tttccacatt
    19381 gttatctgga cgaaaagcat taccagcaat caattcaaca tctgctgtaa tatttttctc
    19441 atcaaacggt aaaatctgaa tcccagcttg ttcaagccca cgttgtgtaa agtagtattt
    19501 ctctacatca ctaccttgaa ccttatgccc catttgatgt aacataagag caagtgcgct
    19561 catacctgat cccttaattc cgataaaatg ataggttttt gtcatgttat ccctcttcta
    19621 tatgtgtcaa attcaattct tgtgccactt ggcgatctcg ttgttgtctg cgctcaggat
    19681 aattgtaaac ctgactcttt cttaaaaagt catacgaatt tttctttata acctgctcct
    19741 gttctctatc ttctttcttc aaagaataga cagctggcat atcagcgaga atatagtctg
    19801 tctgacgtag gttgtccgcc aaacgtgtca accgacctgc cttagattca gttgttgaaa
    19861 cgacaggagg tttcaccaca actttagctg gtgctggttt ttcatctctt aagtaggttg
    19921 ctgatcgttt tttcttcaag tcttctctag cctgttcacg agccaactgc ccctgtgttt
    19981 tcactggaga aggcttcgtt ttagtcagtt gctgaaggcg ttccttgcga gaataatggc
    20041 ttggcttagc ttcaaacaga ggaggcagga gctcatcttc cgtttgtgct actggttttg
    20101 ccgacgcaac tcgctgtgaa tcggtagacc agctaaattc cttttcttga taaggtcccc
    20161 tgatattgct aatcaggtcg ctctcatcat ataaactcat aattggattt tctccaacta
    20221 gtacctcatc atctgcgact agtggaaatc tattttcatg tctaatcata agtcatcctt
    20281 tcgaatcctt attataccat acttaggcct tatttcctaa ttctacgctg agtcaactga
    20341 ttccatatca ctcaatccct aaaatctcaa gaatatcctc aaccgtcaaa ttcgctcggc
    20401 tttcattgcc atcaaggact gttttgacaa ggttcttttt attttcttgt aattcttgaa
    20461 tcttctcttc gattgtccct ctagtaatca ggcgatagca ttcaaccttt tcagtctgcc
    20521 ccatgcgatg agcccggctg atagcctgag cttccacggc aggattccac caaagatcga
    20581 ctaggatgac tgtgtctgca cctgtcaaat tcaagccaac gccacctgct ttcaaggaaa
    20641 tgagaaaggc atctcgactt cctgcattga aggcctgtgt catttcttgg cgttggttag
    20701 caggggttga accagtcaga gtataggatg tcatccccat ttctaccagc tgttcttcta
    20761 ttttttccag catatttctg aattgggaga atatcaagac cctgtgttca ccttctttta
    20821 gctgcaagag taattctttc aaactattaa gtttgccact ctcaccacta aactcttcca
    20881 tgaaaaggct gggggtatcg cagatttgac gtaagcgagt aattcctgat aggatttcta
    20941 tcttgctacg attgatttga gcatcatctg cgccagctat ttgagtccgc atttgttgaa
    21001 gctgagctag gtagatggct ttttgttcat cagttagttc attaagtaca ttgacttcta
    21061 tcaaatcagg caattcctga agaacgtcct ccttatgacg tctgagaacg aagggttgaa
    21121 tggtccgcgc aatatccttg gctgctaatt taccgaagtc ttgtttgcct ggcaataaac
    21181 ctggtaagac aatttggaaa atggaccaga gttccgtcaa atggttttca atcggtgtac
    21241 ctgagagggc aaagcaattc ccaacctcaa actcacgcag aagctgtgca atcttagatt
    21301 gggcattctt catgacctgc gcctcatcta aaatgaggta gtcaaagcgt tcctgtttat
    21361 agagtgcaac atcctgtctg aaggatggat aggaggtaac taagacttgg tgaccattgg
    21421 caataatctc ttcacgctgt tccttgtttc catggacaac agccacatct aaacttggag
    21481 caaaccgctt gcactcatct aaccagttat aaatcaagct agacggtgcc aatattaaaa
    21541 ctttggaatc ttttgtcatc cgactggaca gaaaggcgat agtttgcaag gtctttccaa
    21601 gccccatgtc gtcagctaaa attccgccaa aaccatacgt atccagcata gtcaaccact
    21661 taagccctgt ctgctgataa tccctaagcg acgttgttac ttccacagca ggaagcggga
    21721 acaaatctgg ccgagctaga tagcttgcca tttcctcaaa ttccttggag aaactgactt
    21781 ggttgaattg tgacaaggat tgagccaatt ggtaaccggc aagagcatga acttgtacct
    21841 gcccctcctt actatgacgg gcccgcaaat agattagggt ttgactgatt ttcttggttt
    21901 cctcatcaaa aacaaatacc ttaccgctcg ggctagtata gtgatcctct tgattcagca
    21961 aggctgtaat cgcctgatcg atttcagatt gttcaatacc tgataagtca aacgaaatat
    22021 ccaaaagcga gccgttacgc acaatttcta attgaggttt ggcttcaata tataaagcct
    22081 ggagcctttc tgctagatga acctgcccca ttttttgtaa tataggcaat tgttgttgga
    22141 aaaatggata gagctcctgc tgactaagag ccgctctctg ggcattatat gtccccctaa
    22201 agccagctaa acgaatagcc tgatagacct tctccaaatg ttggaaatgg ctggcaaaag
    22261 gcaggagagc caaatcctct tcactcctta ccttacgtcc atcaaaatcc aaaaccaatt
    22321 ggagtgataa actttcattt gcctccatct gaatatgaaa ttctggcttg aaatcatgaa
    22381 tgatgaagcg tttaggtgcc ttaacagtcc caattgtctg caaatccagc aagcttaaag
    22441 ccagacgatt ttggtcaggg aaatctacct gaacctttct caaacctgat tcggttggaa
    22501 taagttcgga aatttggtaa atcagatgtt cttgatggcg attgacacta taaaccgtgc
    22561 catttactaa gagataacgc ccatgaaaaa gaggtcggac tgttttttcg tgaataacca
    22621 tttcaatcat ctgcgtgtga acggtcacct caaaatgata gagcccttcc tcacctgtca
    22681 acggaagaac catcaaggag gaataggact gctgttgata ggaaaagtta aaatgttcta
    22741 gctgatttaa cagctccaac ccctcctcaa aataggcttg tggcagacga aaatgtcgtc
    22801 caaaatgagt aagaatatcc gaatctatac caactttctc tggtaccaag gcccagagaa
    22861 aatccaggag agcctgactt ggttcatcaa aattttcata agtaacttgc tcataataat
    22921 ttttacctat ctgatagtga ccgttggctt tcacaatacg caaaaaagct ccgatgtccc
    22981 gcacaatgta cgaacgtgta tctggcaagc gggtaatttt caaggtccaa tctatctgac
    23041 gatcataggg caataagctc ccttcaactg ataactgata cttaagcccc agttcaggtt
    23101 ctggccgcaa aatttcatct aaaaagagac cgccaaaata agtccgacgg acggtttcct
    23161 tatgttgttc accatctttt ttcaaagact gcttcatatc cttgcctgag gcatcatttt
    23221 ttaaaaaata ctccgtggca gctagatgtt gacagtagcc cttactttga aataactggc
    23281 aactacactg taaatcgcta tctgcatcgc cgtaagtaaa gcgctcacca gcaatatcca
    23341 gtaccatctt tccattctca ctatgaagaa cagtcagttt tccagcctcg tacaaatcaa
    23401 ttccttcttg gcgaatccgc cctggcatca tgcgacccat tctaactcct ttatccttca
    23461 accaatcatc agctcttctg taaaatatat ctaaaatatt atcttttcat tatagcatat
    23521 tttcaatttg aattgtttaa ataggtatca aaaaaacacc agctggtaaa ctggtgtctc
    23581 taatgaatag ctaaataata aatcgttgct tgccacattt tcaagattgt ttttccgctt
    23641 ttccaaagcc aaaatagggc aaatagcagg aaaacaactg tcactactac gttaatcaag
    23701 ccttttccat ccaagaaaaa cttgaaaaag aagacagtca cacaagtaag caaggcaagg
    23761 agtaaaacat gaatcagaac ccccaaccag cccagccaat agcgaattcc aaagggaggg
    23821 tgtttctttt catcgaagtc tgctagacca gtcaaaaact caactagcaa ctcaccaaaa
    23881 aattccatac tagacttcct ttcttacccg tcgataatac ttggttcgtc gaatcatcat
    23941 cttgatagcc ttaaaggaaa gtaaggttag catactagcc acgaatagac aaaaaaggaa
    24001 tatcacaacc aatggctgtt tatactgatt atagaccata attgaaccac cagtaaaggc
    24061 gataaaatag gatgccaaag tcacaaaatt gagccagata caagccccaa cccccagcca
    24121 atatcgaaga ccgaaaggct tatactgact gtcttcataa aatacaagaa aaaatgataa
    24181 aaaagaaagc aatatcaaaa aagaacgcca aatcggaacc gctaccatac caacacctcc
    24241 aagtccattc tagcatattt atgaataggt gcaaagaaaa aagatgtcgc cctctttatt
    24301 tccgtttccg tgcaatcaaa tgaatcggtg ttccttcaaa tacaaaggcc ttacggattt
    24361 gattttccaa gaaacgcata taggagaagt gcatgagctc ttcttcgttg acaaagacca
    24421 caaaggtcgg tggtttggtc gcaacctgag tcgcgtagaa aatcttgaga cgtttaccct
    24481 tgtcagttgg tgtcgggtta atggcaatgg catccatgat aacatcgttg agaacagccg
    24541 acgggatacg ggtattctgg ctttcgctaa tggccttgat cgtctcaggc aacttatgca
    24601 agcgttgttt ggtcaaggct gacacaaaga taatcggtgc gtaagacaag tattggaact
    24661 ggtcacgaat atcgtcttcc cactgcttca tggtatggtt gtctttttca agcgtgtccc
    24721 acttgttgac tacgataatc atccccttac cagcttcatg ggcaaaacct gcgatgcgtt
    24781 tgtcgtactc acggatacct tcttcagcat tgataaccat caatatgaca tccgagcgtt
    24841 caatggcacg catggcacgc ataacagagt atttctcagt attttcatag accttaccag
    24901 acttgcgcat ccctgcggta tcaatcatgg taaattcttg accttctggg tctgtaaagt
    24961 gcgtatcaat agcatcacga gtcgttccag caacaggcga cgcaatcacg cgctcttcac
    25021 ccaaaatagc gttaatcaaa cttgacttac caacgtttgg acgaccaatc aagctgaact
    25081 tgataatatc tggattttct tctacttcct gagctggtag gttttcaatg atagcgtcca
    25141 agacatcccc tgttccgata ccgtgtacag aagatactgg ataaggatct ccaagaccga
    25201 gtgaatagaa atcgtaaatg tcattccgca tttcagggtt atcgacttta ttgacaacta
    25261 aaataactgg tttgtttgtc ttataaagga tacgcgatac atactcgtca gcatccgtca
    25321 caccttcttt gcctgatacg acaaagacga taacgtccgc ttcatccatg gcaatctcag
    25381 cctgatgctt gatttgctcc atgaaaggtg catccacatc atcaataccg cctgtatcaa
    25441 tcaaggaaaa cttacggttg agccactcac cagtagcata aatacggtcg cgggtcacac
    25501 cttccacgtc ttctacgata gaaatacgtt cccccgcaat acggttaaat agtgttgatt
    25561 taccaacgtt gggacggcca acgatggcga tagttggtag agccatagct cctcctcttc
    25621 tagtttacaa taatttattc ttttctaaga tagactttgt ttcaggctgc tctagctgac
    25681 caaactgagc aaccattcgc tcagaccaag tttctgtctg acgggctcct gcatattccg
    25741 tctgaacttg gtcgtaggct tggattactt ccacgccttg atccacatat tcttcctgaa
    25801 agacaatggt ttcacttgcc aaacgtggtt taacaggatg gttttgggct ggtcgaccaa
    25861 gtgcaatgca gaaaattgga taggtataat ctggcaagtt gaacagctct gctattgcca
    25921 aggccttatg gcgaatcata ccgataaaga ccccaccata acccaggctt tcagctgcta
    25981 aaagggcgtt ttgccctgct aggctcgcat caacagaaga aatcaggaga ttctctgttc
    26041 ctttggcatc aaagtctgaa ccatggagtt gagccgcctt gctggcacga ttatggtcgc
    26101 ccacaaaaac cagaatagcc tgagcctgta aaatagctgg ctggggcacc aaatcataga
    26161 tagcctgttt tttctcctcc gactgcacca caataatgga ataggactgg aaattcttcc
    26221 agctagatgc agctcgccca gctgatataa tagcttgcaa atgttccgcc tcaataggtt
    26281 cctccgtaaa acgacgtacg gaagtatggt tcaacatcaa atcaatggtt tcattcatcg
    26341 gcggttattt cctttcaaat gcaaatcgcg agccagatag tgaatccgct ccataacccg
    26401 tttggcctgc caggtctcat caccagactt gccagaggcg aaatgacgct ccaaatcatc
    26461 aaaggagaaa ttggaggtaa agaaggtcgg cagattttcc tgcatgcggt attgaagaat
    26521 gacctgcagc acatcgtcac gtacccaagg actatgttgc tccgctccga tgtcatctag
    26581 caccaagacc tgagccatct tgatttcatc aattcgctcc ttgacagaac catctttgat
    26641 ggcatttttt atgtctacca caaaggttgg ataatggagc atggtggttg attgaagatg
    26701 tgtcttggac aaaagattgg ctaaataagc catcaaataa ctttttccaa taccgaaatt
    26761 gccatagatg tagaggcctt ttggtttttc ctcaaatcgt ttgacaaaat ctgcaatagc
    26821 ctcataaacc tcaacacgat taatatccgt aaaatccaag tcctcagctg taactttttt
    26881 gagagcctga ggcatattga tgagctggat acgattcttg atggcttcca aacgacggta
    26941 ttcgaccaat tcctctgttt caagatagga cacatccgca tagccctcat tcataaccaa
    27001 aactggttgg taccccttag caatatagga ttcatcctgc ttgacaaaga ggtcacgctg
    27061 actgatatat tccaaaaact tagaaatgga gagggtgatt tcctgctgag tcaaaccttc
    27121 ttttttgata aaagcagcga cttcaggatc actcactatc tcctgataga gttgctgata
    27181 agattttgga cttggattgg ttgcctgcga caacctgtct tgaactgatt tcatcagcta
    27241 cctcccttac tttcgttttc taacattcgt tttctaagtg cttccattct tgcctgagct
    27301 tcaggactgt tatccatgac aaccacttcc ttactccact caggtacatt gcttttctgt
    27361 ggctggctcg acttggtaac aacttgacct gtcttcaact ctcttaggta aagaacagcc
    27421 gcctctgcac tgccaattcc cttgtaggaa aaatcattgc ccagcttcat agcgtatttt
    27481 tcatttagat tggctgagtc caccttgtta aaggtataga ggaccaagac attgataact
    27541 tcgtcaagca gacccaaatt ggccaaatcc gtcaagcatt ttcgttccgt tcctgtcacc
    27601 gctgccttcc gttgctcctt gataaaggat aggaaggtta ggctggattt taatttcgct
    27661 tcacgaacaa tggcttgttc gtgggcagtg aggttggcgc tttctgttgg caatgtctgg
    27721 cgtgactgct gcaagcgttt ggtcgagatg gtctgtccaa tagctgttgc tttagcctgg
    27781 cgataggttt ccaaccaggt ccaccctgcc tgctctgcta tatgactaag ggcaataata
    27841 tcctcagctt catcttgaaa aagaagcttg tccttagcca tcaaggcttt aaaggccgac
    27901 caatcaaaat catgcttggg ttcaaaagct actggaactt gaccatcaat ggtaaagact
    27961 tgggagaagg ttttggatac cttcttctcc ttatttggtc cagatacttg taaactttct
    28021 acactggctt cgccaattct ttgggccaat aaattcttat aaagcggctt gcccaaaaat
    28081 tgtttgaccg tcagtggtgg caacaagacc aagcctatca actcatctgc ccgatagact
    28141 tgtagcaatt ccatggcaga taagagatcc aaggcctgct ccaagcgctt catgccgaaa
    28201 tccatatgat tgaggattgt cgcccacttg taacgtacct gcccctgatc cgagaaacta
    28261 tagaggtagt agtaaagggc tagggcatca aaactaatga taggttgata acactggctt
    28321 aaactgataa tgtctggtgt aaaagggctg gtcttgatgt aggtaaataa atcacttggc
    28381 ttcatgagca gcacctgact ttactttact acccttagaa atcatctgct tgagcagatt
    28441 ttccaactct tctacatcct tgaaggaacg ataaacagaa gcaaaacgca cataggtgat
    28501 ttcatccaac tccaccaatt catccatgac aaagttacca atcacatcac tttcgacctc
    28561 gccatcactc tgagaacgaa ccttctgctc aatccggttg acaacttcgt ctatatcact
    28621 agttgaaact ggacgttttt gtgctgaacg aatgatgcca ttgaaaatct tttcacggga
    28681 aaattgttcc ctagtcccgt ctttcttcac aacaacgagg gttttttcct caatgcgctc
    28741 ataggtggta aaacgttgac cacattgatc gcaagaacga cggcgacgga tggtgttgcc
    28801 atcttcagcc tgacgactat caataacact tgatttcaaa ctttgacact ttggacaacg
    28861 catactctca ctcctacaac ttctttatca cttcagtata gcataaatac agcaaaaata
    28921 aaagaaagcg agaaaactca ctttctattt ccaaccatat ttagctgact gtcatatcct
    28981 ctttcttgtt caatcgactg gtaacccact ctcggactaa gcggtaaatg accgcaaaga
    29041 taggcgtaaa gaagaccata ccaagcaagc caaataaatt tccaccaatc aaggctgctg
    29101 ctagggtaaa gagagtcgga agtccaaccg attgtccaac cacgcgtgga tagataacat
    29161 tcccctcaat caactggata atctggaaga gtgcaatgga ccagagcgcc aagagtggat
    29221 tttgcaccgc cacaaagaga gcactaatca aacatgctga aaagggaccg atataaggta
    29281 caaatgatag gaccccagcc aaaataccag ccatgctggc ataaggaatt cctgagagcg
    29341 aataactaac aaaaacaagt gtcccaatga tacacgcctc aacaatctga ctcatcaaga
    29401 atcggtcata ggtatcaaca ataacttctc caatatagtt cactactctg acagccttct
    29461 ccggcaaggt agcctgcaag aactttctcg tcatcgcctg caagtgttcc ttgctcccta
    29521 aaatcgctag agtgaagaag aaagccatta tcaacgtcat agtgttcgaa aaaatccccg
    29581 tcacattgga caccaagcaa gataaaatcg gtgtgaccaa acttgaaata agtgttaaat
    29641 tcttcaactg atccaaaaag ctggcaatct ggtctccgat ttgtcccgat aaaagaccat
    29701 ttgattcaag aaaattagtt aaggcattta ccgattttgg aatggctgtg ctgctaaccg
    29761 aaaccagttc tgaaacagtc gtcaccaatg taggaagaac aatcaccacc agtcctgtca
    29821 caattaaagc aaatccaatt aatactccaa caattgccaa agaccgcttg gatttcttta
    29881 aaaaagaatt tttttcaaac tgatcttcta attttttcat gggaacattg agaataaagg
    29941 caaaaatagc tccaataaaa agcgaattca tactgcctaa taattgttga atagccccat
    30001 aaatactaga ccagttgaga acagctaaca atgtcgctcc agataagagt atcaatatga
    30061 ctttttcttt aaatgacgta ttcataatat ctccaaattt taaatgatta gttttcagtt
    30121 taacacagga agataaaaaa agaaagtgtt tcctcattga tagattgaag acaaatccat
    30181 ttgtatattt gcgttcaata ttagatataa aaaagattga agaaaattat ccaatgcaga
    30241 cgatttcttc aatctgccaa agctgttttt tacagtctta tttaaaatcc ctaagtaata
    30301 ttgttgagaa aatcaccgtt tgccacgaca gttatacgca gcaatagcta attctgcagc
    30361 tgtagccaaa acatttgctt tacttgtact ttttccaagt aatttaagta tggtcatagc
    30421 cgctcgattc catgcctcac tttgtaaatc tttagccaaa gcgtctaata aacgaccatt
    30481 ggcaatatct ataaatattc caaaataatc agaaactaca cacttagcaa aatcgtaagg
    30541 agttctcata agactagcaa tatacccctg ataaagctta cgagcattct gatctcctga
    30601 tttagctttt tcaagaagca aattaggatt agtaatttga taaacaccat taccatcaat
    30661 gtaacctact tctttaaaat aaaattctaa ctcagcttcc aaaatagcta catctcccga
    30721 acttaaatcc gaatatacag acacttcatt attttgttca gctaaaacaa caattgaact
    30781 aaacggtgca atagtatagt taaatgaaac aaaaccagta catttatgat ataatgtatt
    30841 tatgcatatt cattagattt tcgtaaaaaa gttctcgcat actgtgagaa
//
LOCUS       DATUCG010000010        30055 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.010, whole genome shotgun sequence.
ACCESSION   DATUCG010000010 DATUCG010000000
VERSION     DATUCG010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 30055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30055
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.010"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(<1..755)
                     /locus_tag="VCA20_000384"
     CDS             complement(<1..755)
                     /locus_tag="VCA20_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834251.1"
                     /translation="MNKKNILTGFTLLVSTVLLFACQSNESTTKSSEKIVETSSSSKD
                     AISEEQRNKWLKEFYEEVAETDGSNYHNDIEERSQKAWNENQTNIFNGQASLNVTNDY
                     LPSELTKLNVTIDGYDEATRRLKVTVTNNYDETLIGTTTNSNNNNLYGTYFTLYGYTT
                     LSGRSERVKIAQIALASDLPAEESIQLEILPSALANRQTDYAKKFQNSELDSKYYLLS
                     TNYGNSLWDITKDQVMGEEIISEQIVLSSLANI"
     gene            complement(771..1748)
                     /locus_tag="VCA20_000385"
     CDS             complement(771..1748)
                     /locus_tag="VCA20_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834252.1"
                     /translation="MDIANSLRRYLILKNNLIPSTQNRIKELGREIFQAEVTLNAISD
                     EKVFLEDFKWDIRTRGFTIFNLVHLPSLIVFFIFLIVVLFSIAGIAIIGVIIETIIRT
                     FILFQDFEFEDTFFYIPIKAVEDFIYYPPSIPIPIVVGLLIVWVLYSIRQVRKNRIHY
                     ENVVIPENRKKNALVPGKRRKQQLVVDNLKNTLKSTETQLLNHQNELMNIMNTISIPN
                     QYWTNDEVINFLIQKLEYGQAKTIGKALNLWDIEVSTRRVEQASKAVIKETRESEARL
                     ARQRQEQFIQATKAQQARDEVQEKIAEEMKRKNDHDAIHDNIAKKNGWL"
     gene            1874..2047
                     /locus_tag="VCA20_000386"
     CDS             1874..2047
                     /locus_tag="VCA20_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834253.1"
                     /translation="MAFQLKSNRKETENKTIRFPVPLIEEIEKAIQNKDVTFSSFVIQ
                     ACEYALRDIEDGK"
     gene            complement(2168..3439)
                     /locus_tag="VCA20_000387"
     CDS             complement(2168..3439)
                     /locus_tag="VCA20_000387"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834254.1"
                     /translation="MKKILYLYNQIKSDKNKLLAYKIFFKELIFELFNCDSQEEFEKI
                     PLTDDDVKILLEKLDEKFTTIFSTQEMLNIINSNLYYPCERDDIYRKIKNAVTYAGFI
                     DDYIQKCKEFGITADFNKIIAGVGRPTTKYNIVFWDLFIDFVKNESQKYKKVKSGVRI
                     KNLALLSKELEATIDNKQKYFDIVKSQLDSIEVEYYNDLEKLYKMMFSYVGRAFELYD
                     LLNDEFNQKITEEMGIYLDFVELMTHEFVNLFISKTVGIFEDANLAEQQIAVNYYRGI
                     FTDWTLFFEDFRPLEMKETMMSFRERYRKLYFENSDLRIIPATKSSSIYYFMIVKERL
                     LKTLQDDIDLDYVNVNNHIQRLKDLSTRYVERKIKLTSDTERELYSIYHTQFRSTLLK
                     YIQSKFEKYSSNDLEFQRLKEQLIDRKWLYL"
     gene            3620..4144
                     /locus_tag="VCA20_000388"
     CDS             3620..4144
                     /locus_tag="VCA20_000388"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834255.1"
                     /translation="MKIKFDENVSRVTLQKLSQKCDLVLCSYSEFSKKDFEYKSSTPI
                     DYLRVGYDEREKAPLYLLARDKETGTYYCADSVLQLREQLETTRLEILVDEIAKSQEN
                     CEILEEKRASLRNEVNAINDEIARLNEIKFPLRREIDKLKRTKESLKESFYDWKNDHW
                     LWQFLYWIYEIINE"
     gene            4595..4933
                     /locus_tag="VCA20_000389"
     CDS             4595..4933
                     /locus_tag="VCA20_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834256.1"
                     /translation="MAKTNWNRTLDEVLKHKTQSVVMTSEKTGNEYTTEVIPTLTVLS
                     TGSIEVSDGKFKYSIVDAKNELEYIIKTSNLVDVKFGTTLQFKNVRGGTTPNGIGWYT
                     AESVTLVQQN"
     gene            5126..6493
                     /locus_tag="VCA20_000390"
     CDS             5126..6493
                     /locus_tag="VCA20_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEP1834257.1"
                     /translation="MVKKEAKLTTIMLVQQLENEYWLSSDYQKPLQDAKSGNCRPLLE
                     MIVEKLEKHDIIVKDAYIIKHDKDKVSIWNPYEMKNIAENKEDHVHALFKFEKGASLN
                     KLALAIQVEPQYLEKLKSGRYGYDNCLAYLCHSKQENKHQYQPEEVITVRGEEYISIY
                     HRSIETWMKGRATKKAKETNLSVDWLIEKILAGDLTKSNIMLTDEYYSIYGQHKRKIN
                     EALETAGERRSYRTIAELEAGKFKKTVLFINADSGVGKTQFSKNFIRLLKNVARNFGH
                     SWDSCVTASTNPFDEYNGQEILFLDDIKGDSLTVSDWLKLLDPFMISPISARYHNKMG
                     SAKVIIITNTKEPLSFFEQAKGNISEDLGQFVRRIDCLLTIDKNFNISLPKKTVNPVE
                     ISTHPWIEPKISSYNFANPIALSTNETLDYLIKTIIQNMQWNKKKAISDTKQNTEDNP
                     NIQQK"
     gene            6561..6857
                     /locus_tag="VCA20_000391"
     CDS             6561..6857
                     /locus_tag="VCA20_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEP1834258.1"
                     /translation="MQVILPDEQVHQIQRLVAKLIHQEIENQLTNRNLDSPYLNKKQT
                     CEYLGISNNTLDNWIQKGLPTIRINKTVRFNKNEINRWISNIKKEKDNNVQNTI"
     gene            6838..7095
                     /locus_tag="VCA20_000392"
     CDS             6838..7095
                     /locus_tag="VCA20_000392"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834259.1"
                     /translation="MYKTLFERLSAEELAIIYILLELLLKTVFEDRPPDGIETYQGEF
                     QHYDYANSILEDCITEAVEMYRIKFGNDAEFRDYDILKRPI"
     gene            7252..8469
                     /locus_tag="VCA20_000393"
     CDS             7252..8469
                     /locus_tag="VCA20_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEP1834260.1"
                     /translation="MSITKTKNGTYRLRIYIPEEAKSSLGIDKKVIEKRFKLRSEAKK
                     YELELQNKIDKILSGESTPLETNGTILFSDFYHNVWWESYKAGQTTSTTKPPSQATID
                     GTEIVFRKHILPMLGNYSIDFLNQNKQVVLNLLTQKAEEYANFKVIRSYVNSIFDWAE
                     ELEYIETNRLSKTISRIKATKKINLQESKRDEDLYLSQAELQAWFTAFEEDLESGKLF
                     FKDYVLFYTTFFLGDRKSESYALQWKHINIKKQEIQLVQALDKYKNPKSTKGNKKTTF
                     RIPNELTDLLQSWKKQQKAELAKFGIIQSDEQYVFTYIDMKGNVNSPLHADYLNNKMK
                     SVKRRHKELTHATPHKLRHTGATLAKQAGTSLEAISEALTHSDTITTKTYVNTSNVIP
                     MAVGEIAYRNLKK"
     gene            complement(8579..8971)
                     /gene="rpsI"
                     /locus_tag="VCA20_000394"
     CDS             complement(8579..8971)
                     /gene="rpsI"
                     /locus_tag="VCA20_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEP1834261.1"
                     /translation="MAQAQYTGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLVINQPFAVTSTQGSYDVFVNVNGGGYGGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(8991..9437)
                     /gene="rplM"
                     /locus_tag="VCA20_000395"
     CDS             complement(8991..9437)
                     /gene="rplM"
                     /locus_tag="VCA20_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEP1834262.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKIYYTHSNHPGGLKSISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGAEHTHAAQQPEVLDISGLI"
     gene            complement(9652..10011)
                     /locus_tag="VCA20_000396"
     CDS             complement(9652..10011)
                     /locus_tag="VCA20_000396"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834263.1"
                     /translation="MVPLASRRSSKARGYGQALLEHSLALAENSGAVLVAITGFPDYY
                     SRFGFVKGKEIGIRYQADPEADYFLVQLFRPEALDGRDWWFTDPPGYTVDELVLEEFD
                     KTFPYKEKLALPGQLGQ"
     gene            complement(10362..11255)
                     /locus_tag="VCA20_000397"
     CDS             complement(10362..11255)
                     /locus_tag="VCA20_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HEP1834264.1"
                     /translation="MKRLLSCYASDIVGATKEELKQSILSSEGRILMGETVVTAAPLI
                     AGVTNAEMMATFGCDLLVLNELDVFHPEIVDFYDCDNPIAEIKRLTGRLVGINLEPVD
                     TSQEVQDQQVFLKPGRQVSPESLRQAQVLGVDFIMLTGNPATGVSMAAIRSAIGLAKE
                     HFKGLIFAGKMHGAGLGESVVDSQALLDFVELGADGVLIPAVGTVPGVREEIVAPIVS
                     QVKAHGALVISTIGTSQESADSQTVREFGLSNKRVGSDIHHIGDGGYGRMPDPENILA
                     LSLAVRGKRHTYFRMGQSVRR"
     gene            complement(11318..12178)
                     /locus_tag="VCA20_000398"
     CDS             complement(11318..12178)
                     /locus_tag="VCA20_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002949140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEP1834265.1"
                     /translation="MTFTIVTDSTSDLPISWVQENDVTVLGLTINLDGVTYETVGENR
                     LTSADLLEKMASGSQPTTSQVNVGQFEEVFEAAAKEGHEVLYLAFSAALSGTYQSAVI
                     ARDMVMDQYPEAVITIVDTKAATIGEGYLVMKAVEARAAGKTLAETKAIVEDLVPRLR
                     TYLLVNDLNHLVRGGRLSKAAALIGGLVNIKPLLALNAEGKLEAIAKIRGRKKGIKEM
                     LNLTLDNLDHSTVMVAYTGDLEAAEAIKATLLEDSCVTDVLLTELGPIIATHTGTGVL
                     AILSINKKER"
     gene            complement(12175..12696)
                     /locus_tag="VCA20_000399"
     CDS             complement(12175..12696)
                     /locus_tag="VCA20_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEP1834266.1"
                     /translation="MKEKVLIVDGYNMIAFWQTTKQDFKKGNLDAARTTLLRTLSHYA
                     AFEQIEVICVFDAHHVPGLRQQYDEFKVSVIFTEEEETADSYIERLSAQLNSDRRKQV
                     SVATSDLNEQWVVFSQGALRVSARELEERTRVIKKDLDKFVDQVELYTPRLNPWSDGN
                     FQALKEMMEEMKE"
     gene            complement(12697..13425)
                     /gene="rlmB"
                     /locus_tag="VCA20_000400"
     CDS             complement(12697..13425)
                     /gene="rlmB"
                     /locus_tag="VCA20_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEP1834267.1"
                     /translation="MEHNDIVYGVHAVVESLEANTGNKLYIQDDLRGKNVEKIKALAT
                     EKKVSISWTPKKTLSDMTEGAVHQGFVLRVSEFAYADLSVILEKAEQEDNPLILILDG
                     LTDPHNFGSILRTADATQVAGIIIPKHRAVGVTPVVAKTSTGAVAHVPIARVTNLSQT
                     LDKLKEAGFWVFGTDMNGTPSHKWNTAGKLALIIGNEGKGISSNIKKQVDEMITIPMK
                     GRVQSLNASVAAAVLMYEVFRKKI"
     gene            complement(13481..14686)
                     /locus_tag="VCA20_000401"
     CDS             complement(13481..14686)
                     /locus_tag="VCA20_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEP1834268.1"
                     /translation="MNSFEKQQRHNEKIRSMNAQARAMSAQAIALQKQNNILQKEHER
                     QIEKEFKDRIFQLRLHLAQVTDESQRFVIQQMLSEAEEDYENYQREQVEKCLADAKRR
                     KIWLIISSIVAVLGMAGLYFVYQDISQVTVPELYGIELSEAKDLLYEEGLKVGTITEV
                     GGQSVQPGMVSVSSPSSGKKVKRGSAVNLIIAKNVDGKDTAVVEDGSTSEVATSKTAG
                     SSQASSQSQVGSAVQSFTVDIKKQGLVIRQEPSLSGEVVTNIEPGTYTIVETTQKEGH
                     SWGRLKSGQGWISLTDLHNPVPQVDTKNLTTDQVKAWVMDDLFSRSVMIEKYRKDQVV
                     ISVRMAEDGLVYADVTVPSDPNFEPRDWSYRINSRGELEASEYDTDPEQGGVNWYVAQ
                     HYYLENSVN"
     gene            14944..15333
                     /locus_tag="VCA20_000402"
     CDS             14944..15333
                     /locus_tag="VCA20_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HEP1834269.1"
                     /translation="MTLIFGIVAGLIAFAIGGLWYGLIFRDAWIKASGIDMAKVEADR
                     QAGKNGQKEMVISLVIEVLTAIITIFFIKTLSVSPLHAAGGMGVIAVLASLKNYVFEQ
                     RPINLILINESYKLVCYLVVGILALFA"
     gene            15408..16376
                     /locus_tag="VCA20_000403"
     CDS             15408..16376
                     /locus_tag="VCA20_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEP1834270.1"
                     /translation="MSLSLRTIKLMFATVLAIYLATALGLSYATAAGIIAILSVLDTR
                     KSSFKMARNRLFSTLLALTIAVLTFALFGFGIWTLGIYLALYVPLAYRFNWEAGIAPS
                     TVLVTHLLLEQDISLIFLGNELALFLIGAGLALLFNLYMPSQEKKIQAYHDQVEDLLK
                     QILLRFEAFLLNGDGRNEAELITQLDKTLDEALKVVYLDRHNQLFQQTNYQVHYFEMR
                     AAQNKILRTMAGNINKCLLEGRENVILSSLFERAAQQLSRENSAKELLLDIELFHATF
                     RERPLPQTREEFETRATLFQLLHDMEAFIRLKVDFYEVYKDEKDPS"
     gene            complement(16416..17546)
                     /gene="ugpC"
                     /locus_tag="VCA20_000404"
     CDS             complement(16416..17546)
                     /gene="ugpC"
                     /locus_tag="VCA20_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEP1834271.1"
                     /translation="MVQLNLKNIYKKYPNSEHYSVENFNLDIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDITEGEAYIDGVLMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYSKDEIKKRVEEAAQILGLTEFLQRKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRTEIAKIHRRIGATTIYVTHDQTEAMTLADRIVIMSATKNEAGT
                     GTIGRIEQIGSPEELYNKPVNKFVASFIGSPAMNFFTVTLQEGVLVGDGFKVDLPEGR
                     RKHLEEKGYNGKSFTLGIRPEDIKASQLELDTYPSSIVEAEVVVSELLGAETMLYSKV
                     GNTEFVSRVDARDYHNPGDKVRLTFNLNKAHFFDDETDQAIV"
     gene            complement(17656..18510)
                     /locus_tag="VCA20_000405"
     CDS             complement(17656..18510)
                     /locus_tag="VCA20_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEP1834272.1"
                     /translation="MNEKELAGWFPQAKLSQEADAHSLALGDGRYLNLADADLTERER
                     YLLSLLSHPQEQKLSSPWQSYLHGQSSLPQAVEAIQFIHVHLWSQKEAQLDAWQTMMT
                     DLLPSQLAQVQLSSQDYLFIIEQSRWTDYQTILADTLSALEFDFGLRLTFLVGQVWSR
                     QLADRWSQLYQAEAALFANWRAHYHQSTVLSFSQLFLWGQGRAGLEFGMIKASMLQLI
                     DQQDMQDIILALWTEGAVVTKTAQILYIHRNTLQYRLDKWQDLTGLQLKDLTDLSFCY
                     QVLLDRLF"
     gene            complement(18512..18910)
                     /locus_tag="VCA20_000406"
     CDS             complement(18512..18910)
                     /locus_tag="VCA20_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEP1834273.1"
                     /translation="MTKAVDVNLINGIALAFEGDAVYSMYVRRHLIFKGLTKPNKLHG
                     EANKYVSAKAQAGLISALLEAQRLTEKEEEIYKRGRNTNSYTKAKNADVVTYRMSTGF
                     EAVLGYLHMTEQMERLDELVGWCIDNIERG"
     gene            complement(18903..20246)
                     /locus_tag="VCA20_000407"
     CDS             complement(18903..20246)
                     /locus_tag="VCA20_000407"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000591125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEP1834274.1"
                     /translation="MIKIYDTMTRSLRDFVPLEEGKVKMYVCGPTVYNYIHIGNARSV
                     VAFDTIRRYFEYRGFDVAYISNFTDVDDKIIKAANEAGMTTKELSDKFIAAFKEDVAK
                     LGVKPATKNPRVIDYMDEIIDFVQVLIDKGYAYEASGDVYFRVEKAENYARLANKTLS
                     DLEAGASGRVEGEGQLKEHPFDFALWKTAKSGEVSWGSPWGAGRPGWHIECSVMATEI
                     LGATIDIHGGGADLEFPHHTNEIAQSECRTGQTFANYWMHNAMLNINDEKMSKSLGNF
                     LTAHDMLEQIDGQVLRFFLATQHYRRPLNYTEKAISDAEINLKYLKNTYTQPVQNSVD
                     KSGLESHLAAFEAAMDDDFNAANGITAIFDFAKWINSGNYDETVKEAFGKMLAVFGIV
                     FEEEVLDSEIEALIAERQAARANRDFATADRIRDQLAAQGIKLLDTKDDVRWIRD"
     gene            complement(20257..20442)
                     /locus_tag="VCA20_000408"
     CDS             complement(20257..20442)
                     /locus_tag="VCA20_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834275.1"
                     /translation="MLKKLVRQNWPYVLTAIAGAIMFILKFSQGSWQVGMIWLAATAY
                     WLVKLYQKYQVLKNTQK"
     gene            complement(20436..21209)
                     /locus_tag="VCA20_000409"
     CDS             complement(20436..21209)
                     /locus_tag="VCA20_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HEP1834276.1"
                     /translation="MLLGEFENTSAVIEPTDKAIRGGGEVCDTMIFSFNGEIVDRVSQ
                     LAESREGGHLQTLNGRHPWYILERDGLQVAVMLAVIGAPMAVGHLEELKACGFENFIV
                     LGSCGVLDKSLAADKIILPSSALRDDGTSYHYAPASDEISYDPALLLTMEKTLDQAGI
                     EHVQAKTWTTDAFFRETAAKVKRRLAAGAMVVDMEASAIMAWANFRQAKVYQFFYTAD
                     YVDHHKNEWDARREERTADSMTFFEVAMAIARKLESRSC"
     gene            complement(21211..22071)
                     /locus_tag="VCA20_000410"
     CDS             complement(21211..22071)
                     /locus_tag="VCA20_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEP1834277.1"
                     /translation="MIIATNVLNSQQLSAAKTLISTVQSHDGTYRDPYLSNMLNFDPE
                     MPAFFLAYQGEQLVGLLTVYADDEDVELAIIVHPDYRRQGLARKLYDCYQTKTANYPI
                     ASVTFQTERVFLDMHPDLAPAWNLVEDTDTETWLGREREPYQFSQQAELTVSLAQAHH
                     ADSIARCKSAAFGNDYEVALRYVREAISDADSLLYLLEHSGAVIGSCTVDISTDDNYL
                     YGLAIAPDQQQKGYGTYLVKAVINDLIRKNDKPFQIAVEDDNLIAKRLYENIGFTKQT
                     QVVYLDPKGE"
     gene            complement(22127..22744)
                     /gene="cysE"
                     /locus_tag="VCA20_000411"
     CDS             complement(22127..22744)
                     /gene="cysE"
                     /locus_tag="VCA20_000411"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEP1834278.1"
                     /translation="MGWWKDTIEIVKEKDPAARTTLEVLLTYPGVKALAAHRLSHWMW
                     KKGFKLLARMHSQFWRFWTNIEIHPGAEIASGVFIDHGAGLVIGETAIIESGVMLYHG
                     VTLGGTGKDTGKRHPTVRKGALVSAHAQVIGPVEIGENAKVGAAAVVLADVPADVTVV
                     GMPAKIVRVHGQKDEKVIHDMEDGREHYTTKLAELREASHCSSHL"
     gene            complement(22797..25016)
                     /gene="pnp"
                     /locus_tag="VCA20_000412"
     CDS             complement(22797..25016)
                     /gene="pnp"
                     /locus_tag="VCA20_000412"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEP1834279.1"
                     /translation="MSKQVFETVFAGKKLVVETGQVAKQANGAVVVRYGDSTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGWMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDPDASAPMAAMFGSSLALAISDIPFNGPIAGVQVGYVNGELI
                     INPDKAQMEASLLDLTVAGNKDAINMVESGAKELSEDIMLEALLKGHVAIQELLDFQN
                     QIVAAVGKEKADVELLQVDPELQAEIVAAYNDDLKKAVQVEEKLAREDATNAVRETVI
                     AAYEEKYAEHEEFDRIMRDVHEILELMEHAEVRRLITEDKVRPDGRRVDEIRPLDAEV
                     DFLPNVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLDDEYKKRFLHHYNFPQYSVGS
                     TGRYGAPGRREIGHGALGERALEQVLPSLEDFPYAIRLVAEVLESNGSSSQASITAGT
                     LALMAGGVPIKAPVAGIAMGLISDGTNYTVLTDIQGLEDHFGDMDFKVAGTRDGITAL
                     QMDIKIDGITPQILEEALAQAKKARFEILDVIEATIPEVRPDLAPTAPKIDTIKIDVD
                     KIKIVIGKGGETIDKIIAETGVKIDIDEDGLVAIFSPDRDAIERTKEIIAGLVREAKV
                     DEVFQAKVVRLEKFGAFVNLFDKTDALVHVSEMAWTRVNKPEDLVEIGDIVDVKVIKI
                     DDKGRIDASMKALLPKPEGYVEPEKRERSDKPRRPRDHKEKKDNNFGEFKFHKVEKK"
     gene            complement(25223..26116)
                     /locus_tag="VCA20_000413"
     CDS             complement(25223..26116)
                     /locus_tag="VCA20_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015075113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HEP1834280.1"
                     /translation="MKRLLNCVASDFEGLDREGLLNAIRASEGRVIMAENVTHAGSAL
                     PGVTNAELVRAFGADLILMNTLDVLHPEIPGLDVTDRPIARLRELTGRPIGVNLEPID
                     LEAEMLEERLEINEGRQVNAKTLQALNEQGFDFVCITGNPGAGITNKTIAEAIRFAKT
                     IFKGVIIAGKMHGAGSKDPIFDQEALLSFADAGADIVLIPSPGSVPGSTIETCAQIVG
                     LLKEKGVLTVGGLGTSQESSDIATIKQIGLWNKMVGFDIHHIGDAGFGGMAPLENIFE
                     LSVAVRGRRHTYKMIGTSIKR"
     gene            complement(26113..27369)
                     /locus_tag="VCA20_000414"
     CDS             complement(26113..27369)
                     /locus_tag="VCA20_000414"
                     /inference="COORDINATES: protein motif:HMM:TIGR00410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEP1834281.1"
                     /translation="MKTTFLDKIEQFSYKIGQQKHIIALRDGMVQTMPLILVGSLFVI
                     IQSFPVPAWEAFLTEVGAMSYLGGLITGTFGIMGLVAVFGIAKRLADSYKVDGTSAGV
                     IALAAYLVLVPLQEGMMSTGYFGSRGLFVAVVVGLITGELFRILIQKNIVIKMPESVP
                     PMVAKSFTSLIPAFVIISSFAGIDLLFKALGFTDIFQVMLVILAEPLKGASNTLWGAL
                     LAVLFNSVVWLFGVHGGQLVSSIMDPVWFMNTDANREAWQAGLELPFIVTKPFLDNFA
                     WLGGSGASIGLAIFLFFFAKSQQNKALGKLAIVPDIFSVNEPMLFGFPIVLSLDLAIP
                     FILTPLVTATVSYLAMDWGLVHKTVGIMIPWATPPIISGYLSTGGHISGSILQIVNIL
                     IAVAIYYPFVKRIDNRMRKEEEEAAG"
     gene            complement(27388..27690)
                     /locus_tag="VCA20_000415"
     CDS             complement(27388..27690)
                     /locus_tag="VCA20_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016181419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEP1834282.1"
                     /translation="MINILLCCSAGMSTSLLVTKMQKAADSRGLESTIWAVSADEAGQ
                     NVGKADVILLGPQIKFLRGDIESLAGDKPLDVIPMRDYGMLNGEAVLDLALSLYQS"
     gene            complement(27687..28013)
                     /locus_tag="VCA20_000416"
     CDS             complement(27687..28013)
                     /locus_tag="VCA20_000416"
                     /inference="COORDINATES: protein motif:HMM:NF014326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEP1834283.1"
                     /translation="MFTNVEDSMQIIANSGDARSDYMEAIRLASVGDLQAASQQMDMA
                     KDKIVEAHRLQTELIQGEFRGDKAELNLLLIHAQDHLMSTLVIRDLSRYLLDLYQKLG
                     EKEKEI"
     gene            complement(28003..29808)
                     /locus_tag="VCA20_000417"
     CDS             complement(28003..29808)
                     /locus_tag="VCA20_000417"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012579.1,HMM:NF013070.1,HMM:NF016901.1,
                     HMM:NF019884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEP1834284.1"
                     /translation="MYQREWLILKELEKGGYVTSAQLAQKIGVSVKTVQKSIAHLNES
                     MTKQMIGSIVSKTGQGYRLQIRQQDHYQKLVAELDGRQGISGAEQRQIYLLNELLKQP
                     YVKSEQVEVALYISKKTLSNTVRALRELLERYQIDIKTRPHYGMYLDGQEKDIRLMMV
                     LVEGVELYDLSKEEKIAIEQLLLIQGVQTDFYRANVLSILRATLTRVQRGYGLPEVAV
                     SSKIGLADLSEKYGWGLQTADIVSIEETINSFRVKVSSLASNQQLKLCLSMVEEAFGL
                     DFSRDKDFINSLWSHVQKLHERVKNRVILKNPLLAEIKQNLKNEFVMASLLSGALARE
                     WSAPILEDEIGFLSLIFATCTSQIKDLKKHMLVVCFDSESGKDFLQSTYQRLFGRYIH
                     QVTVCNPEEIAQQDLSAYDCIVSTVELPVLTPYNPHYVSYFLEERDKEAIKQQLTTTE
                     NLFVNQLLEEVIFLDQLPFESKEQLVEDICRHIEQVRGIDVTEKVLKRLSMGVTQLGH
                     KMVLLHPQGKVDEHFVSVTVLDRPVYWESSEVRVVVLASLPTIAPVSRLIYQILSTFM
                     IEDIYMEGLLQVPTADNLQVILQKIKEKELKGYVY"
BASE COUNT         8795 a         6877 c         5757 g         8626 t
ORIGIN      
        1 taaatattcg caaggctaga taatactatt tgttctgaaa taatctcttc tcccattact
       61 tggtctttag taatgtccca taaggaattt ccgtaattgg ttgatagtaa gtagtattta
      121 gaatctaact cactattttg aaatttttta gcataatcag tttgacgatt tgcaagagcg
      181 gatggaagaa tttcgagttg aatagactcc tcagcaggta aatcactagc aagagcaatc
      241 tgagcgattt taaccctctc agaacgtcct gacaatgtgg tgtagccata tagagtgaaa
      301 tatgtcccgt acaagttgtt attgtttgaa ttggttgttg ttccaatcag tgtttcatca
      361 tagttgttag taacagtaac tttcaatctt ctagttgctt catcataacc gtcaatcgtg
      421 acatttagct ttgtcaattc agaaggtagg tagtcattag taacatttag actagcctgt
      481 ccattgaaga tgttggtttg attctcattc catgcttttt gactacgttc ttcaatatca
      541 ttatgatagt ttgaaccatc agtctcagct acttcttcat aaaactcctt caaccactta
      601 tttctttgtt cctcggagat tgcatccttt gaagaagatg aggtttcaac aatcttttca
      661 gaggattttg tggtactttc attactctga caagcaaata agagtactgt agataccaat
      721 agtgtaaagc ctgttaaaat attctttttg ttcatagtgt gtctccttat ttatagccaa
      781 ccatttttct tagcaatatt atcgtgtatt gcatcatggt catttttgcg tttcatttcc
      841 tcagctattt tttcttgaac ttcatccctc gcttgttgag ctttagtagc ttgaataaat
      901 tgttcttgcc tctgtctagc cagacgagct tccgattctc ttgtctcttt tatgacagct
      961 ttcgatgcct gctcaaccct tctagttgat acttctatat cccaaagatt caaagctttt
     1021 ccgatagtct tcgcttgccc atattctaat ttctgtatga ggaaatttat gacttcatcg
     1081 ttagtccaat actggttggg aatggaaata gtattcataa tattcatcaa ttcattttga
     1141 tggttcagta gttgagtttc agtggatttc aaggtatttt tcagattatc taccactaat
     1201 tgttgttttc tacgctttcc agggacgaga gcatttttct ttcgattctc tggaataaca
     1261 acattttcat agtggattct atttttccta acctgtctga tactatataa aacccaaact
     1321 atcaaaagcc caaccacaat cggaatagga attgaaggtg gataataaat aaagtcttca
     1381 accgccttta ttgggatata gaagaatgta tcctcaaact caaaatcttg gaaaagtata
     1441 aaagttcgaa taattgtttc tataatgaca ccaattatgg caatccctgc tatagagaat
     1501 agtacaacta taagaaatat gaaaaacaca atcagggacg gtaggtgaac cagattaaaa
     1561 atagtaaatc ctcttgtgcg aatatcccat ttaaagtcct caagaaatac tttttcatct
     1621 gaaatagcat tcagggtaac ctcagcttga aaaatttctc ttccaagttc ttttattcta
     1681 ttttgtgttg aggggattag attatttttt aatattaagt accttcttaa tgaattagca
     1741 atatccatct gggctcctat attttaattt ttattacatt ataacgtaat aacattaagt
     1801 ttgcaatgca aaacgcaatg catatttaat gtgcaaaatg ctatatccta aaaacaaaaa
     1861 gaggagaaaa gatatggcat ttcaactaaa atcaaatcga aaagaaacag aaaataaaac
     1921 aattcgtttt ccagtaccac ttattgaaga aatagagaaa gcaattcaaa acaaagatgt
     1981 gactttttca agtttcgtga ttcaagcatg tgaatatgcg ttaagagata tagaagatgg
     2041 taaataacaa ctaaaactac tgataagtta tttgagattt ttgaatggaa ctttgaagac
     2101 ataatcaaac atggactaga tactaaaagc cttttcaata atgagaaggc ttttaattta
     2161 ttctatttta aagatataac cacttcctat caattaattg ttcttttagt ctttgaaatt
     2221 caaggtcgtt gctcgaatat ttttcaaatt ttgactgtat gtactttagt aaggttgagc
     2281 gaaattgtgt gtggtaaatt gaatataatt cacgttccgt atcactagtt aactttatct
     2341 tacgttcaac gtatcttgta cttaaatctt ttaatcgttg aatatgattg ttaacattta
     2401 cataatctaa atcaatatca tcttgcaatg ttttaagtaa tcgttctttt actatcataa
     2461 agtaatatat actactagat tttgttgctg gtattattcg taaatcggaa ttctcaaagt
     2521 atagcttacg gtacctctcc ctaaaactca tcatggtttc tttcatttct aacggtctga
     2581 aatcttcaaa gaataacgtc caatctgtaa aaatgcctct atagtaattt actgctattt
     2641 gctgttctgc taaattagca tcttcgaaaa tacccactgt ctttgaaata aataaattaa
     2701 caaactcgtg agtcatcaat tccacaaagt ctaaataaat acccatttct tctgttattt
     2761 tttgattaaa ttcatcattt agtaaatcgt aaagttcaaa agccctacca acatatgaaa
     2821 acatcatctt gtataacttt tctaaatcat tgtaatactc tacttcaata ctatctaatt
     2881 ggctctttac aatatcaaag tacttttgct tattgtcaat agtagcttct agctctttac
     2941 tcaataaagc taagttcttt attctcacac cactttttac tttcttatat ttttgacttt
     3001 cattttttac aaagtcaata aacaaatccc agaatacaat attgtatttt gttgtaggac
     3061 gacctactcc tgctattatt ttattgaaat cagcagttat tccaaattct ttgcattttt
     3121 gaatataatc atcaatgaaa cccgcataag ttactgcatt tttaattttg cgataaatat
     3181 catcacgttc acaaggataa tatagatttg aattaatgat attcaacatt tcttgagttg
     3241 aaaaaatggt agtaaatttt tcatctaatt tttctagcaa aattttaaca tcatcatcag
     3301 tcaatggaat tttttcaaat tcttcttgtg aatcgcagtt aaataattca aatatcaatt
     3361 ctttaaaaaa gattttgtaa gcaagtaatt tgttcttatc acttttgatt tgattatata
     3421 gataaagaat ttttttcatt tttgctgtgc tcctaaagtc gaaaattatt ttgattgtat
     3481 catagatgta taagctagct aataaaaaag aaagcgagag aatttatgat gagacgataa
     3541 agttattata cacgatgcaa agctcgaaaa aaagtgcatg cattttcaaa aaaatattag
     3601 aagggaatga agtactcaca tgaaaataaa attcgacgag aatgtctcaa gagtaacgtt
     3661 gcaaaaactt agtcaaaaat gtgatttagt tttgtgctca tatagtgagt ttagcaaaaa
     3721 agattttgaa tataagtcgt ctactccgat tgattatcta cgagttggat acgatgagag
     3781 agaaaaagca ccgctttatc tcctcgctcg cgacaaagag actggtactt actattgtgc
     3841 agattcagtg ttgcaattgc gagaacagct tgagactact agattagaga tattagtaga
     3901 tgagattgca aaaagccaag aaaattgtga aattttagaa gaaaaacgtg cttcgctaag
     3961 aaatgaagtc aatgcaatca acgatgagat tgctagatta aatgagataa aatttcctct
     4021 gagacgtgaa atcgataaac tcaaacggac caaagagtca cttaaagaaa gcttctacga
     4081 ttggaaaaac gaccattggc tgtggcaatt tctgtattgg atttatgaaa tcataaatga
     4141 gtaaatagca taaattattt atgtttttga tacaaattgc ttagtaattg tttcactcta
     4201 gaaactacgc tttgactgac tatcaagcta aaaatttgaa tgtaagtcgc aaagtgcctg
     4261 ctttttgcga ctggaatgaa aatttttagc tttagtctca aagcattttc taccgtgaaa
     4321 caatgcgaaa ttttattaaa aaattcataa tttaagatat taccaatgat tttcatatca
     4381 atattaaaaa tggtaagaaa gtggattttc catggaagat tttagaactt ttgagcctga
     4441 gtttgttctt cacgaaaaaa tttatcatat tatctaggaa taaactcgtt aagcaatggc
     4501 aaattactaa gaagtagatt ttcatttaaa atgagctaca aagcttttta caataaaata
     4561 ataataacta aacgatagaa agaaggcata tagaatggca aaaacaaatt ggaatagaac
     4621 cctcgacgag gttcttaagc acaagactca atcagtcgtt atgactagtg aaaaaactgg
     4681 aaacgaatat acaacagagg taatcccaac tcttacagtt ttatcaacag gttcaatcga
     4741 agtatctgac ggaaaattta aatactcaat tgtcgacgca aaaaacgagc ttgagtacat
     4801 tatcaaaact tcgaatttag tagacgtaaa gttcggtaca actttacaat ttaaaaatgt
     4861 tcgaggggga actacaccca atggaattgg ttggtatact gctgaatctg taactctagt
     4921 acaacaaaac taaatattta tctaaagatg aataaacgct aattctaaat atctctacgc
     4981 ctgcacggca ctgggcttgt caactgccgt gtctaacaaa aacctagata ggacaagggt
     5041 ttgaaacaac atatcaaatt tcgaaccctt gattttctag ggaatagtta gaggaataat
     5101 aaaaagtcaa aggagtttaa ctattttggt aaaaaaagaa gctaaactga ccacaataat
     5161 gttggtacaa caattagaaa atgaatattg gttatcttcc gattatcaaa aaccacttca
     5221 ggatgctaag tctggcaact gccgtccttt gcttgaaatg attgttgaaa aactagaaaa
     5281 acatgacatt attgtcaagg atgcttatat catcaaacac gataaggaca aagtatccat
     5341 ttggaatcct tatgagatga aaaacatcgc tgagaataag gaggaccacg ttcatgcttt
     5401 attcaaattc gaaaaaggag cttctcttaa taaacttgct ttagcaatac aagtcgaacc
     5461 acagtacttg gaaaaactca aatcgggtag atatggctat gataattgtt tagcttacct
     5521 atgccactct aaacaagaga ataagcatca gtaccaacca gaagaagtaa ttacagtcag
     5581 aggcgaagaa tacataagta tatatcatcg cagtatagaa acttggatga aagggcgtgc
     5641 aaccaaaaaa gctaaggaaa caaatcttag cgttgactgg ctaatcgaga aaatattggc
     5701 tggtgattta actaaaagca atatcatgct tacagatgaa tattactcaa tttatggaca
     5761 gcacaaacga aaaatcaatg aagctttaga aaccgctggg gaaagaagaa gctacaggac
     5821 aattgcggaa cttgaagcag gtaaattcaa gaagaccgtt ctctttatta atgctgatag
     5881 cggagtagga aaaactcaat ttagtaaaaa ctttattcgt ttgttgaaaa atgtagcacg
     5941 taattttgga cattcatggg actcatgtgt aacggcttca accaatcctt ttgatgaata
     6001 taacgggcaa gaaattttgt tcctcgatga tataaaagga gacagcctaa ccgtatcaga
     6061 ttggctaaaa ttactagacc cctttatgat aagtcctatc tccgcacgtt accataacaa
     6121 aatgggttca gctaaagtca tcattattac caatacaaag gaaccactgt ctttctttga
     6181 gcaagcaaaa ggaaatatca gcgaagacct tgggcaattt gttcgtcgta tagattgctt
     6241 actaacaatt gataagaatt tcaacatatc tctaccaaaa aaaacggtaa atccagttga
     6301 aatctcaact catccttgga ttgaacccaa aattagctct tacaacttcg caaatccaat
     6361 cgcattaagc actaatgaga ctctagacta ccttatcaaa accatcatcc aaaatatgca
     6421 atggaacaag aaaaaggcta tctccgacac aaagcaaaac accgaagata acccaaatat
     6481 tcaacaaaaa tagcacactg gcaattttgt tgttgctatt atctcacact aatgctaaag
     6541 attcaagaga ggtaaatagt atgcaagtca ttctacccga tgagcaagtc catcaaattc
     6601 aacgattggt tgctaaatta atccatcaag aaatagagaa tcaactaaca aacagaaatc
     6661 tcgacagtcc atacttgaac aagaaacaaa cttgcgaata tttaggtatt tcaaacaaca
     6721 cattggacaa ttggattcaa aaaggattac caactattcg tataaacaaa acagttagat
     6781 ttaacaaaaa tgaaataaac cgttggatat ccaatataaa aaaagagaag gacaacaatg
     6841 tacaaaacac tatttgaaag actttccgca gaagaactag ctattattta catattacta
     6901 gaacttctcc ttaaaacagt attcgaagat agacctcctg acggaattga aacatatcag
     6961 ggagagtttc aacattatga ttatgcaaat tcaattctgg aagattgtat aacagaagct
     7021 gtcgaaatgt acagaattaa attcggtaat gatgcagaat tcagggacta tgatatatta
     7081 aaacgaccaa tttaaacaga taatcggaac cactaaattt tagaatatta tccacttgat
     7141 aattcttcac aaataattta taaaagtcat agaattaacg agtaaaaatc tgccgtatct
     7201 gctaaaatgt agatacggta tttttctagc tcaattcaca aggagaatac tatgtcaatt
     7261 actaaaacaa aaaatggaac ttatcgctta cgtatttata ttccagaaga agctaaatct
     7321 tcacttggaa ttgataaaaa agtgattgag aaacgtttta agttaaggag tgaagcaaaa
     7381 aaatacgaac tcgaactaca aaacaaaatt gataaaatac taagcggaga atcaactcca
     7441 ttggaaacaa atgggacaat cctcttctca gacttttatc acaatgtttg gtgggaatct
     7501 tacaaagcag gtcaaacaac ttctacaact aaaccaccgt cacaagcaac aattgacggt
     7561 actgaaattg tttttagaaa gcatatccta cccatgcttg gaaattattc cattgacttt
     7621 ctcaaccaaa ataaacaagt cgtcttgaac ctattgactc aaaaagccga agagtatgca
     7681 aacttcaagg tcattagaag ctatgtcaat tctatctttg actgggctga ggaattggaa
     7741 tatatcgaga caaaccgact atcaaaaaca ataagtcgaa ttaaggctac taaaaaaatt
     7801 aatctgcaag aatctaagcg tgatgaggat ttatatcttt cacaagcaga acttcaagca
     7861 tggttcacag catttgaaga agacctagaa agcggaaagc tctttttcaa agattatgta
     7921 ctattctata ctactttttt cttaggggat agaaaatccg agagctacgc tttgcaatgg
     7981 aaacacatca acattaaaaa acaggaaatt caattagttc aagccttgga taaatacaag
     8041 aatcctaaat ctactaaggg aaataagaaa acaacatttc gaattcctaa tgaacttact
     8101 gaccttctcc aatcttggaa aaaacaacag aaagccgaac tagccaaatt cggcattatc
     8161 caatctgatg aacaatatgt atttacctat atcgacatga aaggcaatgt gaatagcccc
     8221 ttacacgctg attatctcaa taacaaaatg aagtcagtca aacgccgtca caaggagctg
     8281 actcatgcaa caccgcacaa attacgccac accggagcaa cactagctaa acaagcagga
     8341 acatcactag aggctatttc tgaagctcta acacatagtg acactattac aacgaagact
     8401 tatgtcaata cttcaaacgt tattccaatg gctgttggcg aaatcgccta ccgtaatctt
     8461 aaaaagtaat ggtgtgaatt tggtgtgaaa agtggtgtga attttgctaa aaaatatcaa
     8521 aaaaacaccc catggtgtga accatgaagt gttgtaaatg ctgtaatgta gctggttctt
     8581 aacgtttaga gaactgacta gctttacgag ctttcttaag acctggtttc ttacgttcaa
     8641 ccatacgagc gtcacgtgta agaagacctg cgcgtttcaa tgaatcgcgg aagtctgggt
     8701 ctacttgaag caatgcacgc gcgataccgt gacggatcgc acctgattga ccaccgtaac
     8761 caccaccgtt cacgttaacg aaaacgtcgt atgaaccttg tgttgaagtt actgcgaatg
     8821 gttggttgat aaccaaacga aggtcagcat gtgggatgta ctcttctaca tcttttttgt
     8881 ttactgtgat tttaccagta cctgggacca aacgtacgcg tgcaaccgcg tttttacgac
     8941 gaccagtacc tgtgtattgt gcttgtgcca tttagattac gtcctttccc ttagataaga
     9001 cctgaaatat caagtacttc tggttgttgt gcagcgtgag tgtgctcagc acctacaaac
     9061 actttcaatt tcatgccttg tgcacgacca agtgtgttgt gtggaagcat acctttaact
     9121 gatttttcaa tcaaacgaac agcgttttta gaacgcaatt caccagctga gattgatttc
     9181 aatccgcctg ggtggtttga gtgagtgtag tagatcttat cagttgcttt tttaccagtc
     9241 aatttcactt tctcagcgtt gataacgata acgaagtcac cagtatcagt gtgaggtgtg
     9301 aaagttggtt tgtttttacc acgaagaacg cttgcaacta ctgctgaaag tcgtccaaga
     9361 ggtacatcag tagcgtctac tacataccat ttacgttcaa cttggcctgg tttagccata
     9421 aatgttgttt tgttcatgat ttctcctata cgaatcgttt ttgtttactg ggcggatgtt
     9481 ccggtccgcg ggtatttgga aggttccggg gcctttcaaa tggggtaaac aataccgtct
     9541 accattttat caaatctact agtctccgtc aagccatttt actttacttt ttttattttt
     9601 tgaaagatag cagagctgct gagaatgaaa gccttatcag gtttactttc cctactgccc
     9661 tagttgtcca ggcaaggcca gcttctcctt ataagggaag gttttatcaa attcttccaa
     9721 gaccaattca tccaccgtgt agcccggtgg atctgtaaac caccagtccc ggccatccag
     9781 ggcttctggt cggaacagct ggaccaagaa ataatcagct tctgggtccg cctgatagcg
     9841 aatgccgatc tccttgccct tgacaaaacc aaatcgactg tagtaatcag gaaagcctgt
     9901 aatggccacc aagactgctc cgctattttc cgccaaagcc aacgaatgct ctaaaagtgc
     9961 ctgaccgtat ccgcgggcct tgctgctccg gcgagatgct aaaggaacca aaggtcagac
    10021 taggcaggcg agtgccgtcc gccgtgatct ccgacgccac atagaggata tgccccacca
    10081 gccgaccatc ggcttccgcg acaagattga ggtcttttat gacatgttgt agcttccgta
    10141 attgatgcac aatcagatgc tcagtagcac tcagataata ggccttcagt ttacttttag
    10201 tacaaggcaa cgagttgcag acagtactgg agtacgacaa aacgagttaa cgatgtaata
    10261 aaagataaac tgacggatga aagacattcc aaaaagcgtc acgtgcttgc tcttcaacca
    10321 cacgatgatc tttttcttct tttctaataa taaatgacat tctatctcct tactgattgc
    10381 cccattctaa aataggtgtg ccgcttgcct ctgaccgcca aggacaaagc caagatattt
    10441 tcagggtctg gcatccgacc gtagcctcca tcaccgatat ggtggatatc cgaccccacc
    10501 cgcttgttgc tgagcccaaa ctcccgcacc gtctggctgt ccgcactctc ctgactggtc
    10561 ccgatagtcg aaataaccaa agccccatgc gccttgacct gactgacaat cggtgccacg
    10621 atttcctccc gcacacccgg caccgttccg accgcaggaa tgaggacacc gtctgccccc
    10681 agctccacaa agtccagcaa ggcttggcta tccacgaccg actctcccag acctgcaccg
    10741 tgcatttttc ctgcaaagat gaggccttta aagtgttctt tggcaaggcc aatagcagag
    10801 cggattgcag ccatagacac acccgtagca ggattgcctg tcagcatgat gaagtccacg
    10861 ccaagaactt gggcctgtcg tagactctct ggactaacct gacgacctgg ttttagaaaa
    10921 acctgctggt cttggacttc ctgactagta tccacaggct ccaagttaat cccaaccaat
    10981 cgacctgtca accgcttgat ttcagcgatg ggattgtcgc agtcgtaaaa atcaacaatc
    11041 tccggatgaa aaacatccaa ttcattgagc accaagaggt cacaaccaaa agtagccatc
    11101 atctccgcat tggtcacccc tgcaatcaag ggagctgctg tcaccaccgt ttcccccatg
    11161 agaatccgac cctcactgga taaaatggac tgcttgagct cctccttagt cgctcccacg
    11221 atatcactag catagcatga caataaccgt ttcataagaa cctccaaatt gatattggtt
    11281 tgtaatcgtt ttcttttgtg tgaataaaga aaagagatta tctctctttc ttattgatgg
    11341 ataggatagc tagaacgcct gtccctgtat gggtggcaat aattggtccc aactcagtca
    11401 agagaacatc ggtcacacag ctatcttcca acaaggtcgc tttgatggct tctgcagctt
    11461 ccaaatcacc tgtataggcc accataaccg tagaatgatc caagttatcc aaggtcagat
    11521 tcagcatttc cttaatccct tttttacgac cacggatttt ggcaatggct tctaacttcc
    11581 cttccgcatt gagtgcaagg agaggcttga tattgaccaa tccaccaatg agggctgccg
    11641 ctttagacag gcgtccacct cgcaccaaat gattaaggtc attgaccaag aggtaagtcc
    11701 gtaagcgcgg caccaaatct tctacgattg ccttggtttc tgccagagtc ttacctgcag
    11761 cacgtgcctc aacggctttc atgactaagt agccttctcc aatggtcgct gcctttgtat
    11821 caacgatggt gatgactgct tctggatatt gatccataac catatctcgt gcaatgactg
    11881 cactctggta ggtccctgaa agagctgctg agaaagcgag ataaagtacc tcatgacctt
    11941 ccttggctgc cgcttcaaaa acttcttcaa actgaccaac attgacctga ctggtcgtag
    12001 gttggctgcc agaagccatt ttctccagta aatctgctga ggtcaagcgg ttttcaccga
    12061 ctgtttcata agtcacgccg tctaggttaa tggtcaaacc aagcacggtc acatcgtttt
    12121 cttggaccca gctgattggc aaatccgatg tcgaatcggt tacgattgta aatgtcactc
    12181 tttcatttcc tccatcattt cttttaaggc ttgaaaattc ccatctgacc agggattgag
    12241 ccgtggggtg tagagttcta cctggtccac aaacttgtcc aagtcctttt tgatgaccct
    12301 ggttctctcc tctaactcac gcgccgaaac ccgtaaggcc ccttgtgaaa agaccaccca
    12361 ttgttcgttg aggtcgctgg ttgcgacgga aacctgcttg cgacggtcac tattgagctg
    12421 ggcgctgagc cgttcgatat aactatctgc cgtctcttcc tcctctgtaa aaatgaccga
    12481 taccttgaat tcatcatact gctggcgaag acctggcaca tggtgggcat caaagacaca
    12541 aatcacctca atctgttcaa aggctgcgta gtgagacaag gtgcgaagca aggtcgttct
    12601 ggccgcatcc aagtttccct ttttaaaatc ttgcttagtg gtctgccaaa aggcaatcat
    12661 gttgtagcca tctacaatga gaactttttc tttcatttag attttcttcc gaaatacttc
    12721 gtacatcagc acagctgctg ctacgctggc gttgagagat tggacacggc ccttcatagg
    12781 aatagtgatc atctcatcca cctgcttttt gatattgctg gagattccct ttccttcgtt
    12841 gccaataata agggctagct tgccagccgt attccacttg tggcttgggg ttccgttcat
    12901 atcggtacca aatacccaga aaccagcctc cttgagctta tctagggttt ggctgagatt
    12961 ggtcactcgg gcaattggga catgggccac cgcgcctgtg gaggtcttag ccacaactgg
    13021 ggtcacgcca actgctcggt gcttgggaat gatgatgcca gccacctgag tggcatctgc
    13081 ggtccgcaaa atcgagccga agttgtgggg gtctgtcaga ccgtccaaga tgagaatgag
    13141 gggattatct tcctgctctg ccttttccaa aatcaccgaa aggtccgcat aggcaaactc
    13201 agaaacccgc aggacaaagc cttggtggac cgcaccttct gtcatgtccg aaagggtctt
    13261 cttgggcgtc caagaaatcg acaccttctt ctctgtcgcc agcgccttga ttttttctac
    13321 attttttccg cgtaaatcat cctggatgta gagcttgttg ccggtgttgg cctccaaact
    13381 ctctaccaca gcatgcacgc catatacaat atcattatgt tccatggttt tattatagca
    13441 tatttttgaa aaggaaaaag ccgagaattt ctcagcttgc ttagttcaca ctgttttcca
    13501 gataataatg ttgggcaaca taccagttga cacccccttg ttccggatct gtatcatact
    13561 cactagcttc caactcccca cgactattga tccgataact ccaatctcgt ggttcaaaat
    13621 tagggtctga tggtacagtt acatctgcat atactagacc atcttcagcc atacgaacgc
    13681 ttatgacaac ctggtctttt cgatactttt caatcatgac gctacggcta aataaatcat
    13741 ccatgaccca agcttttacc tgatctgtcg ttaagttttt ggtatcaacc tgcggtacag
    13801 gattatgcaa atctgtcaac gaaatccaac cctgtcctga ctttaatctg ccccaactat
    13861 gtccttcctt ttgagtcgtc tcaactatcg tgtacgttcc aggttctata ttggtcacaa
    13921 cttctccaga taaactaggt tcctgacgaa taaccagtcc ttgtttttta atatctacag
    13981 taaaagattg aacggcgcta cctacctgag actggctaga agcttgcgaa cttccagcag
    14041 tcttactagt tgctacctca ctagtcgaac catcctcaac tacggcagta tcctttccat
    14101 ctacattttt agcaataatt aagttaaccg cacttccccg ctttaccttc ttcccactac
    14161 ttggagaaga tacactaacc atcccaggct gaactgactg tccaccaact tctgttattg
    14221 taccaacttt taacccctct tcatacagta aatccttagc ttcagatagc tcaataccat
    14281 ataattcagg aactgtaact tgagaaatat cttggtatac aaaatataag ccagccattc
    14341 ctaacacagc cacaatactt gaaatgatta gccatatctt tcgtcttttt gcgtctgcca
    14401 aacatttctc aacttgctct ctttgataat tctcataatc ttcctcagct tcgctaagca
    14461 tttgctggat gacaaatcgt tgactctcat ccgtaacctg tgctaagtgc aatcgtaatt
    14521 gaaaaattct atccttaaac tctttttcaa tttgacgctc atgctctttt tgcaaaatgt
    14581 tattttgttt ttgaagtgct attgcttgag cactcatggc tcgtgcttgt gcattcatac
    14641 tcctaatttt ttcattgtgt cgttgctgtt tctcaaaaga attcattgca ctcaactctc
    14701 cctctcttca tcgcttaaaa tctactccag agcttatagg attggtattg catggtaatg
    14761 caatacctgt tgtacttagt ataacagggc atataaaaaa gtgcctgtgc aaacaattag
    14821 attttacttt tctttaaatc ttccctctat acttcaactc aaatagttgc ccacggttag
    14881 atagtgtggt atccttatgc ttatcaattt gtccgtacaa acggaatcga aacaggagat
    14941 tttatgacac ttatttttgg aattgtggcg ggtctgattg cctttgccat cggtggtttg
    15001 tggtatggtt tgattttccg cgatgcttgg atcaaggctt caggcattga catggctaag
    15061 gtagaagcag accgtcaggc tggaaaaaat ggtcagaagg aaatggtgat ttcccttgtc
    15121 attgaagtac tgactgccat cattactatt ttcttcatca agaccctcag cgtttcacca
    15181 cttcacgcag ctggtggcat gggcgtgatc gcggtccttg cttcattgaa aaactatgtc
    15241 tttgagcaac gccctatcaa cctcatcctc atcaacgaaa gctacaaact agtctgctac
    15301 ctagtagtcg gtattctggc tctatttgca taagcaattc cctcctatcc ttaggaggtt
    15361 tttattttat gatacaatag aaatagacta aaaccaaagg agcccccatg tccctctccc
    15421 tccgcaccat caaactgatg tttgccaccg ttctggctat ttacctagcg accgccctgg
    15481 gcttgtccta tgcgacagct gccggcatca tcgccattct cagcgtcctc gatacccgca
    15541 agtccagctt caaaatggct cgcaaccgcc tcttctccac cctcctggcc ctgaccatcg
    15601 ccgtcctgac cttcgctctc ttcggctttg gcatctggac cttaggcatc tacctggcac
    15661 tctacgtccc tctggcctac cgattcaact gggaggctgg catcgccccc tcgaccgtcc
    15721 tcgtcaccca cctcttgcta gagcaagata tttcgctgat ttttttggga aatgagctgg
    15781 cgctttttct gattggagcg ggactggcac tcctgtttaa cctctatatg ccttcgcaag
    15841 aaaagaaaat ccaggcctac cacgaccaag tcgaagacct actcaagcag attctcctcc
    15901 gctttgaagc ctttctgctc aacggcgacg gacgaaacga ggcagaattg attacgcagc
    15961 tggataagac cttggatgag gccctgaaag tcgtctatct agaccgccac aaccagctct
    16021 tccagcagac caactaccag gtccattact ttgaaatgcg ggcagcacaa aacaaaatcc
    16081 tgcggaccat ggcaggaaat atcaacaaat gtttactgga aggcagagaa aatgtcatcc
    16141 tgtccagcct ctttgagcga gcagcccagc aactgagccg agaaaattct gccaaggaac
    16201 tgcttctgga catcgaactc ttccatgcca ccttccgcga gcgacctcta ccgcaaacca
    16261 gggaagaatt tgaaacccgg gccaccctct tccaactcct acatgatatg gaagctttta
    16321 ttcgcctcaa ggttgacttt tatgaggttt ataaagatga aaaagacccg tcataatagc
    16381 gggtctttgg tgttttatga acaaattata aaagattaaa caatggcttg atctgtttca
    16441 tcatcaaaga aatgagcttt gttgaggttg aaggtcaagc gaaccttgtc tcctggattg
    16501 tggtaatcac gcgcatctac acgagataca aattctgtat ttcctacttt agagtagagc
    16561 atggtttccg caccaagaag ctctgaaacg acaacttctg cttccacgat agaagatgga
    16621 taagtgtcca actccaattg tgaagctttg atatcttctg gacggatacc caatgtaaat
    16681 gatttgccgt tgtagccttt ttcttccaaa tgcttgcgac gaccttctgg aagatctact
    16741 ttgaaaccat ctccaacaag cacaccctct tgaagagtca ctgtaaagaa gttcattgca
    16801 gggctaccga tgaagctggc aacaaatttg tttacaggtt tattgtaaag ctcttctggg
    16861 ctaccaattt gttcgatacg accgattgta cctgtacctg cttcattctt agtcgcagac
    16921 atgataacaa tacggtcagc caaagtcatg gcttctgttt ggtcgtgcgt tacgtagatc
    16981 gttgttgcac caatacgacg gtggatttta gcaatctctg tacgcatgga tacacgcaat
    17041 ttggcatcca agtttgacaa aggctcatcc atcaagaata ctttggcgtc acggacaatg
    17101 gcacgaccca tggcaacacg ttggcgttga ccaccagaaa ggtcagcagg tttacgttgc
    17161 aagaattctg tcaaaccaag gatttgtgct gcttcttcta cacgtttttt gatttcatcc
    17221 ttgctgtatt tacgcaattt caaaccgaaa gccatgttgt caaatacagt catgtgtggg
    17281 taaagggcat agttctggaa taccatggca atgtcacggt cttttggtgc cacatcgttc
    17341 atcaacacac cgtcgatgta agcttcacct tctgtaatat cttccaatcc ggcaatcata
    17401 cgaagagtag ttgatttacc acatcctgaa ggacctacga aaacaataaa ctctttatct
    17461 ttaatatcta gattaaagtt ttcaacggaa taatgctcgc tatttggata ttttttataa
    17521 atatttttta aattaagttg aaccatgttc aagcctcact ttctttgata gttttattgt
    17581 aaatgaaagc gttttatttt tctaggtcaa ggtgaacaaa aagaacacaa tcttttgtac
    17641 aagttgcaca aaagattaaa aaagtctatc tagtaatact tgataacaaa aacttaaatc
    17701 agtcaggtct ttgagctgga gcccggtcaa atcctgccac ttgtccaatc ggtattggag
    17761 ggtattacga tggatataga ggatctgagc agtcttggtg accacggctc cttctgtcca
    17821 cagggctaga atgatgtcct gcatatcttg ctggtcaatc aactgtagca tactagcttt
    17881 tatcatacca aactccaaac ctgctctgcc ttgtccccaa agaaagagtt gactaaagga
    17941 taggacggtt gattgatggt agtgcgcccg ccagttagca aagagggctg cttctgcttg
    18001 atagagttga gaccaacgat ctgcaagttg acgagaccaa acctgaccga ctaggaatgt
    18061 caaccgcaag ccaaagtcaa actccaaggc tgatagggtg tctgccagaa tggtttgata
    18121 gtccgtccaa cgactttgtt cgatgataaa aaggtaatcc tgactcgaca gttgtacctg
    18181 agccaattga ctgggtagga ggtcggtcat catggtctgc catgcatcca actgggcctc
    18241 cttctgcgac caaaggtgga catggataaa ttgaatagct tcaactgcct ggggcagaga
    18301 gctctgtcca tgtagatagg actgccaagg gctagatagt ttctgctctt gaggatggga
    18361 caacaaggac aagagatagc gttctcgctc tgtcaaatcc gcatccgcca gattcagata
    18421 gcgaccgtcg cctaaagcaa gcgagtgtgc gtccgcttcc tgcgataact tagcctgcgg
    18481 aaaccaacct gcaagttcct tctcgttcat cttatcctct ttctatattg tcaatacacc
    18541 aacccaccag ttcatctagg cgttccatct gctctgtcat gtgcaggtat cccaagaccg
    18601 cctcaaatcc tgtggacatc cgataggtga ccacgtccgc attcttggcc ttggtgtagc
    18661 tattggtatt gcgcccccgc ttgtagatct cttcctcttt ttcggtcaat cgctgagctt
    18721 ctaagagggc agaaatcagg cctgcctgag ccttggccga gacatatttg ttggcctctc
    18781 catgcaattt gttaggcttg gtcagtccct tgaaaatcaa gtgccgtctg acatacatgg
    18841 aatagacagc atccccttca aaagccaggg caatgccgtt gatgagattg acatctactg
    18901 ccttagtcac gtatccacct cacatcatcc ttggtatcca agagcttgat gccctgggct
    18961 gccaattgat cacgaatacg gtcagccgtc gcaaagtccc gattggcacg cgctgcttga
    19021 cgttctgcta tcaaagcttc aatctcgctg tccaagactt cttcttcaaa gacaatgcca
    19081 aagacggcga gcatcttgcc aaaggcttct tttacagttt catcgtaatt gcctgagttg
    19141 atccatttgg caaaatcaaa gatagccgta atcccatttg ccgcattgaa atcatcatcc
    19201 atggctgctt caaaggctgc caaatgcgat tcaagacccg acttatccac agagttttgc
    19261 acaggttgtg tataagtatt tttcagatat tttagattga tttccgcatc tgaaatagcc
    19321 ttctcagtgt agttgagtgg acggcggtag tgctgggtag ctaggaagaa gcgcaagacc
    19381 tgaccgtcaa tttgttccaa catatcatgg gctgtcagga aattgcccaa ggacttggac
    19441 atcttctcat cgttgatgtt gagcatggca ttgtgcatcc agtagttggc aaaggtttga
    19501 cccgttctac actcagactg ggcaatctca ttggtatggt gaggaaattc caagtccgca
    19561 ccaccaccgt gaatatcaat ggtagctccc aaaatctctg tcgccatgac cgaacactcg
    19621 atatgccaac ctggacgacc cgctccccaa ggacttcccc aagaaacttc gcctgacttg
    19681 gcagttttcc acagggcaaa atcgaaaggg tgttctttca gttgaccttc tccctcaaca
    19741 cgaccagaag cacccgcctc taagtcagac agggttttgt tggccaaacg agcataattc
    19801 tccgcctttt ccacacggaa atagacatct ccagaagctt cataggcgta acccttgtca
    19861 atcaagacct gcacaaaatc aatgatttca tccatgtagt cgatgacacg aggatttttg
    19921 gtagcaggct tgacacccag ttttgccaca tcttccttga aagctgcgat aaacttatca
    19981 gacagttcct tggttgtcat gccagcttca ttagcagctt taataatctt gtcatccaca
    20041 tccgtaaagt tagaaatgta agctacatca aagccacggt attcaaaata gcgacggata
    20101 gtatcaaaag ccacgacact acgggcatta ccgatatgga tatagttgta aaccgtcgga
    20161 ccgcaaacat acatcttgac cttaccctct tccaagggca caaaatcacg caaactgcga
    20221 gtcatggtat cgtaaatttt aatcatagtt tctccattac ttctgagtat tctttaggac
    20281 ttgatacttt tgatacaatt ttactagcca ataagctgtc gctgccaacc aaatcatccc
    20341 tacctgccag cttccttggg aaaatttaag gataaacatg attgccccag caatggcggt
    20401 caacacataa ggccagtttt gtcttactaa tttttttaac atgatctact ttctaatttt
    20461 cttgcaatgg ccatggccac ttcaaagaag gtcatactat cagccgtccg ttcctctcga
    20521 cgggcatccc attcattctt gtggtggtcc acatagtctg ccgtgtagaa aaattgatag
    20581 accttggcct gacgaaagtt tgcccaggcc atgatagcag aagcttccat atcgaccacc
    20641 atggcaccag cagctaggcg gcgcttgacc ttggcagctg tttcccgaaa gaaagcgtcc
    20701 gtggtccagg tcttagcctg cacatgctcg attcccgctt gatccaaggt cttttccatg
    20761 gttaatagca gagcagggtc atagctgatt tcatcactgg caggagcgta gtggtaactt
    20821 gttccatcat cacgcagggc agagctagga aggataatct tatccgcagc aagcgactta
    20881 tccagcaccc cacaagaacc aagaacgata aagttctcaa agccacaggc tttcagttcc
    20941 tccaagtgac caactgccat gggagcaccg attactgcca acatgaccgc aacctgcagt
    21001 ccatctcttt ccaaaatata ccaaggatga cgaccattta gggtctgtaa atgaccacct
    21061 tctctgctct cagctagctg acttactcgg tccacgattt ccccattgaa ggagaaaatc
    21121 atggtgtcac atacctcacc gccaccacga atagccttat ccgtcggttc aataactgca
    21181 gaggtgtttt caaattctcc tagtagcata tcattctcct tttgggtcta ggtaaactac
    21241 ttgtgtctgc tttgtaaaac cgatattttc atacagccgc ttagcgatga ggttgtcatc
    21301 ttcgacggcg atttgaaaag gtttgtcgtt tttcctgata aggtcattga tgacagcttt
    21361 gactaagtag gtgccgtaac ctttttgttg ctggtctggt gcgatagcta aaccgtaaag
    21421 atagttatca tcggtcgata tatctactgt gcaagaaccg ataaccgctc cgctatgttc
    21481 caaaagataa agtaaactat ccgcatccga aatggcttca cgcacgtaac gaagagccac
    21541 ctcatagtcg ttaccgaatg ccgcagactt acagcgtgca atactgtctg catggtgagc
    21601 ctgtgccaaa cttactgtga gttcagcctg ttgtgagaac tgataaggct ctcgctcacg
    21661 tcccagccat gtctcagtat ccgtatcttc taccaagttc catgctggcg caaggtctgg
    21721 gtgcatgtca agaaagacac gttcggtctg aaaggtcaca ctagcgatag gatagtttgc
    21781 cgtcttcgtc tggtagcaat catagagctt gcgtgccaga ccctgtctgc ggtagtctgg
    21841 atgcacaata atcgcaagct ccacatcctc atcatcagca tacacagtca agagtcccac
    21901 caactgttcg ccttgataag ccaagaaaaa agctggcatt tcagggtcaa agttgagcat
    21961 atttgacagg taagggtcac gataagttcc gtcatgggat tgtacagtgc ttatcagagt
    22021 ttttgctgcg gataactgct gggaatttag gacattagtg gctattatca tgcttatgtt
    22081 tcctctctgt ggctaaatca cataggactt accatatgtg gctttcctac aagtgcgatg
    22141 aacagtgact agcttctctc agctctgcta atttagtcgt gtagtgttca cgaccatctt
    22201 ccatgtcatg aatgaccttc tcatcttttt gaccatgcac acggacaatt ttagctggca
    22261 taccgaccac tgtcacatct gctggaacat cggctaggac aacggcagct gcacccactt
    22321 tagcattttc tccgatttca acaggtccaa taacctgtgc gtgtgcagaa accaaggccc
    22381 cttttcgaac ggttggatgg cgtttgcccg tatcctttcc tgttccacca agcgttacgc
    22441 cgtggtaaag catgactcca gactcaataa tagctgtctc accaataacc aaaccagctc
    22501 cgtggtcaat gaaaaccccc gaagcaatct cggcgcctgg atgaatttca atattagtcc
    22561 aaaaacgcca aaattggctg tgcatcctag ctagtaattt aaaacctttt ttccacatcc
    22621 agtgggacag acggtgggca gccaaggcct tgacacctgg gtaggtcaaa agaacttcca
    22681 aggtggtccg tgctgccgga tctttttctt ttacaatctc aatggtgtcc ttccaccaac
    22741 ccatagtcac tccttctagc gaaagacaag cccgctaccg aacttgtcta ctcagcttat
    22801 ttcttttcaa ccttgtggaa tttgaattcg ccaaagttgt tatctttttt ctctttgtgg
    22861 tctcttggac ggcgaggttt gtctgaacgc tcgcgttttt ctggctctac atagccttct
    22921 ggttttggaa gaagagcctt catagaagcg tcaatacgtc ccttatcatc aatcttgata
    22981 actttgacat caacaatatc gccaatttca accaaatctt ctggtttatt gacacgagtc
    23041 caagccattt cagaaacgtg aaccagagca tcagtcttgt cgaaaaggtt gacaaaggca
    23101 ccgaatttct ccaaacgaac cacttttgct tggaagactt cgtccacttt tgcttcacga
    23161 acaagacctg cgatgatttc cttggtccgt tcaatcgcat cgcgatctgg tgagaagata
    23221 gccacaagac catcttcgtc aatatcgatt ttcacgccag tttctgcaat aatcttatcg
    23281 atggtttcgc cacccttacc gatgacaatc ttaatcttat ccacatcaat cttgatagtg
    23341 tcaatcttcg gtgcagttgg tgccaagtca ggacgaactt ctgggatagt cgcttcgatg
    23401 acatcaagga tttcaaaacg tgccttctta gcttgtgcca aggcttcttc caagatttgc
    23461 ggtgtgatac cgtcgatctt gatgtccatt tgaagggctg tgataccgtc acgagtacca
    23521 gctaccttga agtccatgtc gccgaagtgg tcctcaagac cttgaatatc ggtcaagacg
    23581 gtgtagttgg taccgtctga aatcagaccc atggcaatcc ctgcaactgg tgccttgatt
    23641 ggcacaccac ctgccatgag ggcaagggta ccagctgtga tagaagcctg tgatgaagaa
    23701 ccgtttgact ccaagacttc tgccaccaat cggatagcgt aagggaagtc ttctaggctt
    23761 ggcaagactt gctcaagagc acgctcacca agggctccgt gaccaatttc acgacgacct
    23821 ggtgctccat aacgacctgt tgatcccact gagtattgtg ggaagttata gtggtgtaag
    23881 aagcgtttct tgtactcatc atccaaaccg tcgatgattt gggtttcacc cattggggct
    23941 aaggtcaaga cagaaagagc ttgcgtttga ccacgggtaa agagacctga accgtgaacg
    24001 tttggtaaga agtccacttc tgcatccagc gggcgaatct catcgacacg gcgtccatct
    24061 gggcggacct tgtcttctgt aatcaaacga cgaacctctg cgtgctccat gagctccaag
    24121 atttcatgaa cgtcacgcat gatgcggtca aattcttcgt gttcagcgta tttttcttcg
    24181 taagctgcga tgacggtttc acgaacggca ttggtcgcat cttcacgggc caatttttct
    24241 tctacctgca ccgctttttt caaatcgtca ttgtaagctg cgacaatctc agcctgcaat
    24301 tctgggtcca cttgaaggag ctccacatct gctttttcct tgccgactgc tgctacgatt
    24361 tgattttgga agtcaagaag ttcttggata gccacatgac ctttcaaaag agcttccaac
    24421 atgatgtctt ctgataattc tttggcacca gactctacca tgttgatggc gtctttgtta
    24481 ccagccactg tcaagtcaag gagggacgct tccatctgag ccttgtcagg gttgatgatg
    24541 agttcaccat tgacatagcc gacttggaca cctgcgattg gaccgttgaa aggaatgtct
    24601 gagattgcca atgccaatga agaaccaaac atagctgcca ttggagcaga agcatctgga
    24661 tcgtaagaaa gcactgtgtt gatgacttgg acttcgttac ggaagccttc cgcaaacatt
    24721 ggacgaatcg gacggtcaat caaacgagct gtcaaggtcg catcggttga tggacggcct
    24781 tcacgcttca tccagccacc tgggaatttc ccagcagcgt acattttctc ttcatagttg
    24841 acttggaggg ggaagaagtc tcccgtcgcc attttttttg acataactgc cgctgtaaga
    24901 actgtggagt caccgtagcg aacgacaacc gcaccgttgg cttgtttggc aacttgacct
    24961 gtttcaacga ccaatttttt gccagcaaaa accgtttcaa atacttgttt tgacatgaag
    25021 ttctcctatt tagataaatt tagtatcgtg ttttctacaa aagtcaagcc agaatcgctc
    25081 cctgcctcac ttttgcacaa aaccacgtac taaattctat tgtagcataa ttttgaggaa
    25141 atgaggcaaa atttctctaa aagaaaagcc actccaacgg agcagccttt cgaggataac
    25201 aaatactttt aactcgctta acttaacgct tgatagaggt cccaatcatc ttgtaggtat
    25261 ggcgacgacc acggaccgca accgacagtt caaaaatatt ttctagcggt gccatgccac
    25321 caaaaccagc atctccaata tgatggatgt caaagccaac catcttattc cagagaccaa
    25381 tttgtttgat agtcgcaata tccgaggatt cctgactcgt tcctagacct ccgacagtga
    25441 gaacgccttt ctctttcaaa agccccacaa tctgcgcaca cgtttcaatg gtgcttcctg
    25501 gcacagaccc cggcgatgga atcaagacaa tatctgcacc agcatccgca aaggataaca
    25561 gagcctcttg atcaaaaatt ggatctttag agcctgcccc atgcatcttc cctgcgataa
    25621 tcactccctt gaaaatggtc ttggcaaagc gaatagcttc tgcaatggtc ttatttgtga
    25681 tccctgcacc tgggttgcct gtgatacaga caaagtcaaa tccctgttcg ttaagagctt
    25741 gcaaggtctt tgcattcacc tggcgacctt cattgatctc caagcgctct tctagcatct
    25801 ctgcttctaa gtcaattggt tctagattga ccccaatcgg tcgacctgtc aattctctca
    25861 aacgagcaat cggccgatct gtcacatcca agcctggaat ctctggatgg aggacatcca
    25921 aggtattcat caaaatcaag tcagcaccga atgctcgtac caattctgca ttggttacac
    25981 caggcaaggc agaacctgca tgtgtcacat tttcggccat gataactcga ccttcgctgg
    26041 cccgaatcgc attgagcaat ccttctctat ctaggccttc aaaatcactg gctacgcagt
    26101 tgagtaaccg tttcatcctg cagcctcctc ttcttccttg cgcatccggt tatcaatacg
    26161 tttgacaaat ggatagtaaa tcgctactgc aatcagaata tttacaatct gtagaataga
    26221 accactaatg tgcccacctg ttgataaata accactaatg atcggtggcg ttgcccatgg
    26281 aatcataatc ccaactgttt tgtgaaccag accccaatcc atggccaagt atgaaaccgt
    26341 tgctgttacc agtggagtta aaataaatgg aattgccaaa tcaaggctca gtacaatcgg
    26401 gaagccaaac agcatcggtt cgttaacaga gaagatatct ggaacaatcg ccaattttcc
    26461 gagcgcctta ttttgttggc tcttggcaaa gaagaagagg aagatggcta aaccaataga
    26521 ggcaccacta ccgcccaacc aagcaaagtt atcaaggaat ggcttggtca cgatgaaggg
    26581 caattccaga cctgcttgcc aagcttcacg gttggcatct gtattcataa accacacagg
    26641 gtccatgatg gagcttacca attgaccacc gtgaacacca aagagccata caactgagtt
    26701 gaacaaaaca gccagcaaag ctccccataa ggtattactt gcacctttca gtggctctgc
    26761 caaaatgacc agcatgactt ggaagatatc tgtgaaacca agggccttga agagcaaatc
    26821 aataccagca aagcttgaga taatcacaaa agctggaatc aaagaagtga aggacttcgc
    26881 caccatcggt ggtacgcttt ctggcatctt aatcacgatg tttttctgga tgaggatgcg
    26941 gaaaagttca cccgtaatca agcctaccac aactgccaca aagagtccac gtgacccaaa
    27001 gtaaccagta ctcatcatcc cttcttgaag agggactaaa acaaggtaag cagccaaggc
    27061 gatcacacca gcactggtac catccacctt gtaactatcc gccaaacgct tggcaatccc
    27121 aaatactgca accaagccca tgatgccaaa ggtacctgtg atcagaccac ccagataact
    27181 catggctcct acctctgtta aaaaggcttc ccaagcaggg acagggaaac tttgaatgat
    27241 cacaaagaga cttcccacca aaatcaatgg catggtctga accattccat cacgcaaggc
    27301 aataatgtgt ttttgttgac caattttgta agaaaattgc tcaatcttat ccaaaaacgt
    27361 tgttttcata caactaacct ccttttatta cgattgataa agtgacaagg ccaaatccaa
    27421 gacggcttca ccattgagca taccgtagtc acgcatcgga atcacatcca agggcttatc
    27481 tccagccaga gactcaatat ccccacgcaa gaacttaatt tgcggcccta gcagtataac
    27541 atctgcctta ccgacatttt gtcccgcttc atccgcagat accgcccaaa tagtggactc
    27601 caaaccacga ctgtcagccg ccttttgcat cttggttacc aagaggctcg tggacattcc
    27661 agccgaacaa cacaacaaga tattgatcat atctctttct ccttttctcc taatttttga
    27721 tatagatcca atagataacg tgataagtca cgaatcacca aggttgacat gagatgatct
    27781 tgggcgtgta tgagcaagag attaagctct gccttatctc ctcggaattc tccctgtatc
    27841 agctccgtct gcaagcggtg ggcttctaca atcttatcct tggccatgtc catttgctga
    27901 ctagctgcct gcaaatcccc tacgctagcc aagcgaatcg cttccatgta gtccgaccta
    27961 gcatcacctg aattggcaat aatttgcatc gaatcttcca cattagtaaa catacccctt
    28021 caactccttt tccttaattt tttgcaaaat cacttgtaaa ttgtcagcag ttggtacttg
    28081 caagagcccc tccatataaa tatcttcaat cataaaagtc gacaaaattt gataaatcaa
    28141 tctgctgaca ggagcaatcg tcggaagaga cgctagcaca accacgcgca cctctgaact
    28201 ttcccagtaa acaggacgat ccaatacagt aaccgaaaca aagtgttcgt ctacctttcc
    28261 ctgtgggtgc agaagaacca tcttatgccc taactgagta acccccatag aaaggcgttt
    28321 gagaactttt tctgtcacat caatcccacg cacctgctca atatgccgac aaatatcttc
    28381 taccaactgc tccttactct caaagggtag ctgatccagg aaaatcactt cttccagcaa
    28441 ctgattcaca aagagatttt cagttgttgt caactgctgt ttaatcgctt ccttatctcg
    28501 ttcctccaaa aagtaggata catagtgtgg attataagga gttaatactg gcaattcaac
    28561 ggtcgagaca atgcagtcat aggccgacaa gtcctgctgg gcaatctctt ctggattgca
    28621 gacagttact tggtgaatgt agcggccaaa aagacgctga taagtggatt ggaggaagtc
    28681 ctttccgctc tcactatcaa aacacaccac caacatatgc tttttcaagt ccttaatctg
    28741 tgaagtacag gtcgcaaaga tgagggataa aaaaccaatc tcatcttcta aaataggtgc
    28801 actccattct ctagctaaag cccctgacag tagactggcc atgacaaact catttttaag
    28861 gttctgtttg atttcagcta atagcggatt ttttagaatc actcgatttt tcactcgttc
    28921 atgaagcttt tgcacatgag accagaggct attgataaaa tccttatccc tactaaaatc
    28981 caaaccaaag gcttcttcta ccatagacaa acaaagtttg agctgctgat tactcgctaa
    29041 agaagatacc ttgacccgaa aagagttgat cgtttcttct atgctgacaa tatctgctgt
    29101 ctgcaagccc catccgtatt tctcagataa atcagccaaa cctatcttac ttgaaacagc
    29161 cacctctggt aagccatacc ctcgctgaac ccttgtcaag gtcgctcgta aaatagacaa
    29221 gacattggca cggtaaaaat ccgtctgtac accttggatg agtaagagtt gctcaattgc
    29281 tattttctcc tctttggata aatcgtatag ctctacccct tccaccaaga ccatcatcaa
    29341 gcggatatct ttctcctggc catccagata catcccatag tggggacgtg tttttatatc
    29401 aatctgatac cgctccaata attctcgcag agcacgaaca gtattggaca aggttttttt
    29461 agaaatataa agggctactt ccacttgttc tgacttgaca tagggctgtt tcaataactc
    29521 attaagcaag taaatctgac gttgctcagc accagatata ccctgcctac cgtctaattc
    29581 agccactaat ttttggtaat ggtcttgctg acgaatctgc aaacgatagc cctgtcctgt
    29641 tttggaaaca atggaaccaa tcatctgttt ggtcatgctc tcgttaagat gggcaatgga
    29701 tttttgaacc gtcttgacac tcacgccaat cttttgagcc agttgagcag aggttacata
    29761 gcctcctttt tctaattcct taaggataag ccattctctt tgatacatat caacctcctt
    29821 atctttattg taaccgcttt tatttttttc ttcaatagcg acttttacct taatcattaa
    29881 ggaaattttt gcttcaaaac aactaaaaac agcgaaataa atcgctgtta aaagaatact
    29941 acctctaccg gacccatata taggggaaga aaggcaacaa aatcagtatg gaaatgaccg
    30001 agaagatggc attatctctt ttttacggca cacaaaaagc ccttgcgggc ttttt
//
LOCUS       DATUCG010000011        28830 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.011, whole genome shotgun sequence.
ACCESSION   DATUCG010000011 DATUCG010000000
VERSION     DATUCG010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28830
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.011"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(<1..818)
                     /locus_tag="VCA20_000418"
     CDS             complement(<1..818)
                     /locus_tag="VCA20_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEP1834285.1"
                     /translation="METANKKFRYSIRKFKVGVGSVLIATCLLGAGVSTPTAFAATET
                     STATETTKTPLQKLQDLVKELETDLAKLNEFPEYDRSEYSFQKSLWDFYLEFGKAEMG
                     RVSTLKEDAPEISRISGLLSDFKNHITMERLTREVAQYRKKYPGNENIEKEYDAHLKQ
                     DKDKSTASQEGDTLREYVDGEASKAMDRIKQRVAELEKSKQPQPSPADELAPAPKEEE
                     TPAPKEEDTPAPDAAPAPAPTPEVDPAPTPIPDAPTPKVEEETQEPKTEEKAPET"
     gene            complement(948..2117)
                     /locus_tag="VCA20_000419"
     CDS             complement(948..2117)
                     /locus_tag="VCA20_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEP1834286.1"
                     /translation="MFRLAKEELEKLGAIITATEIYQQPGLWKEAYQLYFDQLEKIES
                     FLKSIKEKHDFVHVVFTGAGTSDFVGQSIVNYLDQVNDLKHIRFSAIGTVELVCRPHD
                     YLQADVPTVLVSFARSGNSPESVAAVEVAKQLIDELYQVTITCAPEGKLALAAEGDAE
                     NLLLLQPAGSNDKGFAMTGSYSCMALTALLVFSPASQEEKAAWVETIARLGQDVLDRE
                     DYVQDLINLDFERVIYLGSGGFYGLAHEAQLKILELTAGKIATMYESPLGFRHGPKSF
                     INEKTLIFLFASNDAYTRQYDVDLLNEVHGDGIAERIVALSADKLVGTEAANFVLAEG
                     GADLPDVFLTFPYIIFAQTVSIMAAIKCNNFPDTPSPTGTVNRVVQGVIIHPLVK"
     gene            2395..2694
                     /locus_tag="VCA20_000420"
     CDS             2395..2694
                     /locus_tag="VCA20_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEP1834287.1"
                     /translation="MMNMQNMMRQAQKLQKQMEKSQAELAATQFIGSSVQDLVTATFT
                     GDKKLVSIDFKADVVDADDLETLQEMTIQAVNAALTKVDEATQKKLGAFAGKLPF"
     gene            complement(2733..4493)
                     /locus_tag="VCA20_000421"
     CDS             complement(2733..4493)
                     /locus_tag="VCA20_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEP1834288.1"
                     /translation="MKKIQREFQKIYYNLDKILLLFFTLFTFMEFVWIPLNSWISEGL
                     LAMTGHAYLSPTNLLSVFAENLLVTGLFILLFFVNIAIAYLELALLFTGVWQLLDEKV
                     KHLPDYLRDVRDSMVAIIRHSSLPKVLFLLFYSVILLPFLRRVLNIYYFNKIVVPQFI
                     VDYLSNTIWLGILILIFLLLFFWLAARFMYALPNIYFEHRGVKESIAYSWEKTRGRKQ
                     IGSLFRLIWLITFPVLLFTGVGGLFYLVQLLAEHYVPSASYWLAVVCYALLKLAYYGV
                     VALFMMGFVSLLTGKQLPIYRRKRLRHRLRLAILLVSGLVFGTQGALALYFPFDTLPV
                     TISHRGVDDGNAVQNSIEALEKTSQLKPDYIEMDVQETKDGQFIVMHDTDLLALTGNP
                     GGTHDYTLAELTGMTASENGMAAPVPSFDEYLEKADELGQKLLVEIKVTKADSPKLTQ
                     NFLKKYGQRLIAKGHQMQSLDYRTIIEIKQYSEKLVSFFILPFNSIYPTTVADGYTME
                     YTSLDQNFMVKSWIRGKSVYAWTPNEEESMTKMLLLQVDGIITDNMTDLQSLMEEMKE
                     NRHYPDLFFLQFQSLIYNFE"
     gene            4573..5121
                     /locus_tag="VCA20_000422"
     CDS             4573..5121
                     /locus_tag="VCA20_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEP1834289.1"
                     /translation="MIRIAPMTEDYLHTLWKIGYREAAPRWKEFAAPYFDDYQACATF
                     EDFQALELYQWLQKDCVRAILVDQLPIGFVSYYWECQPTRWLEIGIEIYDDKVWGKGY
                     GTAAIQLWIDTIFQQFTELEHIGLTTWSGNKGMMRTAEKLGMTKEAHIRKVRYWQGHY
                     HDSIKYGILREEWVEQKKKSSS"
     gene            complement(5142..5636)
                     /locus_tag="VCA20_000423"
     CDS             complement(5142..5636)
                     /locus_tag="VCA20_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEP1834290.1"
                     /translation="MGIEKTVSELAEILGVSRQAMNNRVRSLPEEFVDKNDKGVTVVN
                     RSGLIKLEEIYKTTIFEDEPISDEVKHRELMEILVDEKNAEIIRLYSQLKAKDKQLAE
                     KDEQLKVKDVQIAEKDKQLDQQQQLTLKAMADKEVLKLELDEVKAQAEEVQAKGFFAR
                     LFGR"
     gene            complement(5806..7207)
                     /locus_tag="VCA20_000424"
                     /pseudo
     CDS             complement(5806..7207)
                     /locus_tag="VCA20_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636759.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            complement(7434..8588)
                     /locus_tag="VCA20_000425"
     CDS             complement(7434..8588)
                     /locus_tag="VCA20_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEP1834291.1"
                     /translation="MSVHKYQSTKGVTYFVKVYLGLNDYGKKKYYTKRGFKTRKAAKA
                     HETAVNHQLNSGTFVHLTTQTTYTYQELYQKWYEAYKDTVEATTAAKTADLYRLHILP
                     VFGEKKISKISPLDCQTFITNKAKTFKNMKQIKSYTSKIFEFALNMNYVERNPMSKVI
                     MPKLKKTVSENYWTVNELHQFLTIILENEPYKHYALFRLLAYSGLRKGELYALKWEDF
                     DSENQLLTVSKSLGRIDGHAIEKGTKNTFSVRSIYLDDETCSILNKWKQETSKEKGQL
                     SVQPLSLDKEFMFTYCNRDGEIEPLHADYINNILKRIIRNHHLKKISPHGFRHTHANL
                     MIEMGIDPVNTAKRLGHASSQMTLDTYSHSTKSGEKQSIKKFADYLNKAK"
     gene            complement(8643..9161)
                     /locus_tag="VCA20_000426"
     CDS             complement(8643..9161)
                     /locus_tag="VCA20_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP1834292.1"
                     /translation="MTNHIAKLIEESGKKLTEISSATNIAYPTLSGYNQGIRQPKKGN
                     AQKLADYFGVSMAYILGLDDNKQAPSTMNIITDSFKISEISSVTPFKSDMEKLKKGIE
                     LGEIHLSMPLNDGFSDDFRRILARYLINHEEEFMKTFTKHMNSLKRESEIWQTWIQTE
                     EYRIRQEDRHKK"
     gene            9308..9592
                     /locus_tag="VCA20_000427"
     CDS             9308..9592
                     /locus_tag="VCA20_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEP1834293.1"
                     /translation="MSEEATLVVQLPKVLEKQLRAHYDEMVSTAIERALEDKELYKPM
                     VRMAALSRWLDVSTTTLQKWTREGMPTMVIDGVTLYDKRAVTRWLKQYER"
     gene            9601..10086
                     /locus_tag="VCA20_000428"
                     /pseudo
     CDS             9601..10086
                     /locus_tag="VCA20_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643974.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaD"
     gene            10138..10425
                     /locus_tag="VCA20_000429"
     CDS             10138..10425
                     /locus_tag="VCA20_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEP1834294.1"
                     /translation="METLWHRELTRQEKVLLEGLTVSDSLLQLAIHKTAELEPKKQNM
                     SYVKGILMNWEARGFITVDQVIASEKDYRPLEISNVEVSEDFIAAMDIWKD"
     gene            10440..10730
                     /locus_tag="VCA20_000430"
     CDS             10440..10730
                     /locus_tag="VCA20_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834295.1"
                     /translation="MTVYYQLENMLVAGFKSQEEYDRYQALKRDYEGESEDYSFSIRE
                     ITNQIEIICKTSENDFPDLEEGILADYMELVAQLKRYDTKQADKYLRMVRHG"
     gene            10723..11085
                     /locus_tag="VCA20_000431"
     CDS             10723..11085
                     /locus_tag="VCA20_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEP1834296.1"
                     /translation="MVERVRNILKKFYVTELQNDVLNRLVNDTGLGSFSNYARRMLFK
                     ETSLFIQFDESQFEELIYSLRRVENNLRQLSSIAEQSQNIQAYRAIEYSRRLVSNYEK
                     QLTHYYKQKKRKLLSKGA"
     gene            11085..11441
                     /locus_tag="VCA20_000432"
     CDS             11085..11441
                     /locus_tag="VCA20_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEP1834297.1"
                     /translation="MVKRRIRNVIKQVFLSEEENQKLLNRMKQDGFSNFSIFARKQLL
                     KPDFETWLVSFPEYQTLTNRLLFIGRTINSIAKSATQFGKISPQDLMELGQLMEELVE
                     HVEKQIREDKQRVAKK"
     gene            complement(11547..12824)
                     /gene="ltrA"
                     /locus_tag="VCA20_000433"
     CDS             complement(11547..12824)
                     /gene="ltrA"
                     /locus_tag="VCA20_000433"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEP1834298.1"
                     /translation="MSQLLDNILSRSNMLEAYNRVKANKGSAGVDGVTIDDIDDYLRQ
                     HWRSTKRLIKQRKYKPQPVLRVEIPKPNGGIRQLGIPTVMDRIIQQAIVQVLSPICEP
                     YFSETSYGFRPNRSCEKAIIKLLEYLNDGYEWIVDIDLEKFFDTVPQDRLMSLVHNMI
                     QDGDTESLIRKYLHSGVVINGQCHKRLVGTPQGGNLSPLLSHIMLNELDKELENRGLH
                     FVRYADDCVIAVGSEAAAKRVKYSVSRFIEKRLGLKVNMTKTKIVRPGKLKYLGFGFW
                     KSSEGWKSRPHQDSIQGFKRKLKKLTTRKWSIDLDSRIEKLNWLIRGWINYFALTNMK
                     STMLSIDERLRTRVRVIIWKQWKKKSKRLWGLLKLGVPKWIADKVSGWGDHYQLVAQK
                     SVLKRAISKPVLVKRGLVSCLDYYLERHASKVN"
     gene            14067..14633
                     /locus_tag="VCA20_000434"
     CDS             14067..14633
                     /locus_tag="VCA20_000434"
                     /inference="COORDINATES: protein motif:HMM:NF014566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEP1834299.1"
                     /translation="MKENKKLQILVQCLFQVMISNICLILYVLFCSVFLKTQSILLSL
                     STPTIVALVVQPIFLEKVLFKSKVNFSKHIIVVISALVSILICSFVNIPENIILILHF
                     ILIGYSEEILYRMIILDRMKSSYNILESIVITALIFAFLGHISEPILDNLMIRFPLGI
                     FLAFIRIRFNNVGIPTIIHALYNVLVTF"
     gene            15087..16289
                     /locus_tag="VCA20_000435"
     CDS             15087..16289
                     /locus_tag="VCA20_000435"
                     /inference="COORDINATES: protein
                     motif:HMM:NF015983.1,HMM:TIGR04085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEP1834300.1"
                     /translation="MKTDEMYLLFNFSNGIVAGVDSQAIEFLKRVKTHQLNCNDLTDE
                     EKEFCTYLVENDFISDVEFKNEQPPVTAYLHLTNKCNLHCVGCYSFDERRNRCEDLST
                     EEILNAIDQLSDVGIQNLVFSGGEPLLRKNIIQIVEYAKIVAKIPNVILITNGTIFNK
                     KILSELSKFVDTVSVSLDTYCSDSEPYLRDKGIFDKIMRSISWLKESGNNVNIIPTLH
                     HLNSSNLLEYVKLAKEIGTTISFSILSACFEGELMSYLPNDTDLINISEFMIESDAPI
                     NDSALEDKTLVARNYCGAGTSMIAIGTQGNVYPCHMLMYDEYCIGNIKYDSIKNMREK
                     SANARLFKSLNVDKIEGCSQCQFRYLCGGGCRARSKLKFDVVDKIDPYCRLFKTYYNK
                     VATEIISN"
     gene            16301..17998
                     /locus_tag="VCA20_000436"
     CDS             16301..17998
                     /locus_tag="VCA20_000436"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012235.1,HMM:NF012869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1834301.1"
                     /translation="MKRILREERNKIVVFIISTFILGLVEVSFATIIQKFIDSLESSN
                     ITKFKLAIFSFVVFLIVNYILNLFVIWIQSVLTKNIHLYLKSNLAKYFLSLGYCDFSE
                     TTTGEKLNCFDYKISLIEQYYLDSIFAMIQATLVLMIATVYLYQISPLICVIVFTLGI
                     VSMLIPLLLGKKLNNLVSENADLSGEYLHFIKEMFQGILAVKTFQKERTFFEKFEEKL
                     FRFEKNAQLLNVKNGEYNQISGFFQYVIIISCLCFGGFLVLNQGLTLGKMIAVTQISN
                     LIVGPIQQIGSSVIDILASKDITKEMSDIFSKQSIVLDKKVYQKGFDNIRVSTNSVTK
                     DNKKLLGGVNLSLEYGKKYCIIGPNGSGKTTFGRLMLNLLSNVEERVFIDGEASNLED
                     VAKLISYVPQNSIAFKGTLKDNITLFERYDDKTIDKVIRDVNLNNKSKEIQMISENGD
                     NISGGELRKVNIARAFLEDRPILLVDEFESALDEESSKLIADKFLSSEKTVIMISHNL
                     DQSFLQRFDSIIFMENGEVTAMEKYEVLLKNCDNFYKYLIHTAPGGGNIHEYNTTSV"
     gene            17973..18839
                     /locus_tag="VCA20_000437"
     CDS             17973..18839
                     /locus_tag="VCA20_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003093752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1834302.1"
                     /translation="MSIILRACNLSKSFDSQEVLTNINFDIQSGDIVGLIGKNGCGKT
                     TLMKMILGLTSRTEGDIQFEGDSNYHTNRKALNKIGFLLDCRLFEDFSAYDNLKLFSM
                     YSSTVNEENLDDRIDRLLKFVGLDNSKKLVKSYSFGMKQRLGLALALLDDPEFLILDE
                     PFVGLDPTGVRVLLDYIVKLRQEKGVTILISSHQLHEIEEICDYFFLINDKRIETHSK
                     LGENQIVITLGNLVPELEHSLRDMVDMKEDQITLPEDMSLLNSTLKLIYKYNGEINDI
                     TINDSTEELFKG"
     gene            18844..19596
                     /locus_tag="VCA20_000438"
     CDS             18844..19596
                     /locus_tag="VCA20_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834303.1"
                     /translation="MKSIILAELYKLLKRKNFIILAVLSLWSLIYGLGAFLHWDFVKI
                     GAKLDLVTFITTMWTFLLILTIPLLLILYTSSFILGGEIQGGQILLEVNRVSSKKTLI
                     ISKYIVVILSILFLYVLNCLSSLITYICFLSRSEFGYVNFWELHPTNMQSIIISLVNL
                     GFIILLASVAFYLSLYVSAIVAAFASLGVYFLCQLLSYVTIISKFVPGYFFVVANYHF
                     SFGLSMIHLLSYFSICILLLVITIKGFEKKAL"
     gene            19634..20362
                     /locus_tag="VCA20_000439"
     CDS             19634..20362
                     /locus_tag="VCA20_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEP1834304.1"
                     /translation="MYRIAICDDDSLQVNNLENQISRYFDEFNVQYEIDGYYKGDRLV
                     KSMKEQMDNYQLIFLDIEMEGINGIETAKLLRNLDKSFILIYVTSYEQYALESFEVSP
                     FRYLIKPVSIEKLEAVLRDVLVELTAKQKFLFYQVGMEHFQVNLDNIVCLYSEFGRKI
                     HLELVDGGNVSFYGKISEIEERLPQTHFIRVNSGTMINLNHVTSLNRSDVTMINSDCI
                     TISRSRKKIVISAYSQFIERSFGL"
     gene            20359..21612
                     /locus_tag="VCA20_000440"
     CDS             20359..21612
                     /locus_tag="VCA20_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004183380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEP1834305.1"
                     /translation="MINLFNSIGLLSLFLFQSFIIWIYLQHFFVIRIPKGKFYCFILG
                     VYFIGRTSITVNHDTIIDFKFFSLFFTFLILFFFLSGDVMKKIFHYLFLIFWFLIQED
                     ITMLLLNHAQQTRAIIIFVVQFLLMLLTLLFILGLKHFKIDNLLGLNKIEYGILSVTP
                     LLSIILLLHKVSLPIDKVLIFDSYLLLINIVIIVLYNYLSEKNYNIIKNKIAFDENSM
                     TCEIIKQEEELATLRHDLKNIVSSIDFYADSSDYNNIKVITREILGQEVFNRKITGCI
                     PIDAVLNQKKSKMKKEGIPYNLDLQIPYNLDLSTIAIDSCAILGNILDNAIEEVERNG
                     LEESIEIALRFKNNKLVFKVANPIKDSNVDVNYDGMKSIKSPNRQGIGIKSSYDRAMK
                     LKGHLNITVQSNQFIVLVVIPLRMN"
     gene            21767..22006
                     /locus_tag="VCA20_000441"
                     /pseudo
     CDS             21767..22006
                     /locus_tag="VCA20_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611996.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            22450..23241
                     /locus_tag="VCA20_000442"
                     /pseudo
     CDS             22450..23241
                     /locus_tag="VCA20_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942232.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
     gene            23661..24836
                     /locus_tag="VCA20_000443"
     CDS             23661..24836
                     /locus_tag="VCA20_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEP1834306.1"
                     /translation="MNTVDTIIIGAGPAGMMAAISSSFYGKKTLLLEKNKRLGKKLSG
                     TGGGRCNVTNNGTLEDLLAGIPGNGRFLYSVFSQFDNHDIMNFFQENGVKLKVEDHGR
                     VFPTTDRSQTIIKCLEMKMLENGVDIRTGTEVVSVRKMDDLFHVKTSEEVFTAPQLIV
                     TTGGKTYPSTGSTGFGHDIARHFKLEVTEIEAAESPVLTDFPHKKLQGISLDDVTLTY
                     GKHVITHDLLFTHFGLSGPAALRLSSFVKGGETAFLDVLPTHSDQDLFEHLEINREKS
                     VKNALRELMPDRLADFFAENYDCKVKQVSQKDLTNLVTLLKALPIKITGKMSLAKSFV
                     TRGGVDLKEINPKTLESKKVPGLHFAGEVLDINAHTGGFNITYCLATGWVVGSSYSS"
     gene            25138..25836
                     /locus_tag="VCA20_000444"
     CDS             25138..25836
                     /locus_tag="VCA20_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1834307.1"
                     /translation="MQLKLSSISKKFDHKIILEDANFQFEQGKIYGLLGRNGAGKTTL
                     FNCIARNLTLDNGSIQFVKGEETLDYDNTDIGFTQTYPQLPAFMTAFEFVRFYMDIHK
                     DKLKSPRSPEEWLNLVGIGEEDQHRLLKDFSHGMQNKVQLLLSLIVQPPVLLLDEPLT
                     SFDPVAAHEFKQLIREAKKDSVIIFSTHILQLAQDLCDEIVLLHHQELQAVPSDKLHD
                     PDFEQEVVALLTAD"
     gene            25846..27468
                     /locus_tag="VCA20_000445"
     CDS             25846..27468
                     /locus_tag="VCA20_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834308.1"
                     /translation="MKTILRYSWYQEKYNLHRSVNGFFYYLRKLPLVGKCIPESIFKS
                     YSFKSSLFLVLHILSIPSRFLVKGFWLLFHFYHASFWMNMLVNGELTFWKILPNTWLL
                     GFMFWLLFVGLSYRLGKSLDPVISKANREFMQNFSLSQSSFLQSQLFIEPILTTIYYL
                     PALTIFCMITKEWLVFPIGLMTVLAGHLAGHALNRLLFERRIFSRRNSWHAWLWIAFV
                     FVLYVLAIVFRNQLSLGMLVPILLVQPFLIWFSYGYLKQQTNHLDYLSYCMDESLQMD
                     KKLFEMTKGNEYTRQGLQMQAKLNAKTGKDLSHLSGMTYLNALLFDRYKKVLYKKVQN
                     WVLAVVVILAALEGIRYFLDPFTFTEAYLLQFLPLSFMIMYVASSGKVVAQMVFVNCD
                     ISMLHYPFYREAKTIIAGFNYRFLQTCKLNLIFATSLFLAIMVLGRFAFSLETILLTA
                     LLLISLTALFSFHDLFIYYILQPFTNDMDVVNPLYKFLSGALYWVAYLNTRINVGSHT
                     YILLVSLAMIAYVSIGYWILLKKAPQTFRLKA"
     gene            complement(27689..28249)
                     /locus_tag="VCA20_000446"
     CDS             complement(27689..28249)
                     /locus_tag="VCA20_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEP1834309.1"
                     /translation="MTIKYPDGTIDLLSPVEVVKQADKTAPTVANDGKGNIVIVPSEK
                     AVEIVVSYVDNNGKPQTVVVTKGTDGLWTASNTVVIVDPVTGQVIVPGSVIKLGTVVT
                     AYSKDEVGNSSDSAEAEVVAVDENNSAAGVKVKSVTTNANNVEKKAKQLPNTGEEANS
                     ATSLGLVALGLGLALLAAKRRRDEEA"
     gene            complement(28286..28798)
                     /locus_tag="VCA20_000447"
                     /pseudo
     CDS             complement(28286..28798)
                     /locus_tag="VCA20_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921768.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
BASE COUNT         8725 a         5230 c         5545 g         9330 t
ORIGIN      
        1 gtctctggtg ccttctcttc tgttttcggc tcctgtgttt cctcttctac tttcggtgtt
       61 ggagcgtctg gaattggagt tggagctgga tccacttccg gtgttggagc tggagctggg
      121 gctgcgtccg gtgccggagt gtcctcctct tttggagctg gggtttcttc ctcttttgga
      181 gctggagcta gctcatctgc gggacttggc tggggttgtt ttgatttttc cagttcagca
      241 actcgttgct taattctgtc catagcctta cttgcttctc catcaacgta ttctcttaag
      301 gtatcgccct cttggctagc tgtactcttg tctttgtctt gctttaaatg cgcatcatat
      361 tctttctcaa tgttttcatt accagggtat tttttacgat actgagcaac ctcacgtgtc
      421 aaacgttcca ttgtaatatg gtttttaaaa tcagataata gtccagaaat cctagaaatt
      481 tcgggtgcat cctccttcaa agtagaaaca cgccccattt cagcttttcc aaattctaaa
      541 taaaaatccc acaagctttt ttgaaacgag tactcagaac gatcatattc aggaaattca
      601 ttcaattttg ccaagtccgt ttctagctct tttacaaggt cttggagctt ttgaagaggt
      661 gtttttgtag tctctgtagc tgttgaagtc tctgtcgccg caaaagcggt tggtgtcgag
      721 actcccgcac caagtaagca agtagcaatc agcaccgagc ctacgccgac tttaaattta
      781 cgaatactat atctgaattt tttatttgca gtttccattt ctaccattcc taattatatt
      841 ttattatata ttattattgt accatgatac tcaggtggtt ggatgtgttt tttatgtgtt
      901 aaaactctac cgaatagctc tgtttctcca tcattgaata gatgtgacta ttttactaaa
      961 ggatgtataa tcacaccctg caccacacgg ttgacggtgc ctgttggtga tggtgtatct
     1021 ggaaagttgt tacacttaat ggctgccata atagataccg tttgggcaaa gatgatgtaa
     1081 gggaaggtta ggaaaacatc tggcaaatct gctccacctt ctgccaagac aaagttggct
     1141 gcttctgtac cgactaactt atcagccgac aatgccacta tccgctctgc tatgccatca
     1201 ccatgaactt cattaagcaa gtccacatca tactgacgag tgtaggcatc gtttgaggca
     1261 aagaggaaaa tcaaggtctt ttcattgatg aaggattttg gtccatgacg gaaaccgagt
     1321 ggtgactcgt acatggtcgc aatcttacct gctgtcaatt ccagaatttt caactgagct
     1381 tcgtgggcaa gaccatagaa accacctgaa ccgaggtaaa tcactcgctc aaaatcaagg
     1441 ttaatcaagt cttgcacgta atcttcacgg tccaaaacat cttgacctag acgggcaatg
     1501 gtctcaaccc atgcagcttt ttcttcttga ctggctggcg agaagacgag gagggctgtc
     1561 agagccatac agctgtaaga acctgtcatg gcaaagccct tatcgtttga accagctggt
     1621 tgcaagagca ggagattttc agcatctcct tcagccgcta gtgccagctt accttctggt
     1681 gcacaggtaa tggtcacttg atacaattca tctatcaatt gcttagcgac ttcaactgct
     1741 gccacacttt ctggtgaatt gcctgaacgg gcaaaggata ccaaaaccgt tggcacatct
     1801 gcctgtaaat agtcatgtgg gcgacaaacc agctccactg taccaatggc agaaaaacga
     1861 atgtgtttca agtcattgac ttggtcgagg tagttgacaa tactttgccc aacaaagtca
     1921 gaagtccctg cacctgtaaa aacaacgtga acaaagtcat gtttttcctt gattgatttt
     1981 agaaaactct cgattttttc caattggtca aaatagagtt gatacgcttc cttccataag
     2041 cctggctgtt ggtaaatttc ggtcgccgta ataatagctc ccaacttttc aagttcttct
     2101 tttgctaaac ggaacatggc tcctcctccg tgatttctgg ttataaccag tttttcttat
     2161 attatagcga atttacccct caaagtcaat ttaaaataga ttaatatgtc aaatttatat
     2221 ccgatattgc tgactataac ttattttaag aaggtagcat tatctacata aaacatgtta
     2281 taatatttac tacgcctctg cctttgaaaa tttttaacaa taaggcaaaa taaggcaagg
     2341 aaaaattaca aacagggtct aaacccttga taggaaagga atcttaaaaa tcttatgatg
     2401 aacatgcaaa acatgatgcg ccaagctcaa aaacttcaaa aacaaatgga gaagagccag
     2461 gctgagttgg ctgctactca atttattggt agctctgtac aggacttggt tactgctact
     2521 tttactggag ataagaaatt ggtttctatc gacttcaagg ctgatgttgt ggatgcagac
     2581 gaccttgaaa ccttgcaaga aatgacaatc caagccgtca acgctgcact tacaaaagtt
     2641 gacgaggcaa ctcagaaaaa actaggtgcc tttgcaggta aattaccttt ttaaaaagaa
     2701 aacgcgattg gatactctcc tctcgcgttt ttttattcaa aattataaat caaagactga
     2761 aattgcagga aaaagaggtc tggataatgt cgattctctt tcatttcctc catcagactt
     2821 tggaggtcgg tcatgttgtc agtgatgata ccgtccactt gtagcagcaa catcttggtc
     2881 atactctctt cttcattcgg tgtccaagca taaactgact tcccacgaat ccaagatttt
     2941 accataaagt tttgatcaag tgaggtatac tccatagtat agccgtcagc aaccgtcgtc
     3001 ggatagatag agttgaacgg aagaataaag aaggatacta atttctcgct atactgcttt
     3061 atttcgataa tagtacggta atctaatgat tgcatctggt gtcctttggc aatcaaacgt
     3121 tgaccgtatt tttttaagaa attctgggtg agttttggcg aatctgcctt ggtcacttta
     3181 atttctacca ataatttctg tcccagctca tccgcttttt caagatattc atcaaaagaa
     3241 ggaactggag ctgccatgcc gttttctgat gcagtcatcc ctgtcaactc tgcaagagta
     3301 tagtcatgtg tacctccggg attgcccgtc aaagccaata aatccgtatc atgcatgaca
     3361 ataaactgac catccttcgt ctcctgaaca tccatctcaa tatagtctgg cttgagttga
     3421 gaggttttct ctagggcctc gatagagttc tggacagcat tgccgtcatc gacacctcgg
     3481 tgagaaatgg taaccggcag agtgtcaaag ggaaagtaga gggcaagcgc tccttgggta
     3541 ccgaatacca gaccagatac gagcaaaatg gctagtcgaa ggcgatgacg gaggcgttta
     3601 cggcggtaga ttggtagttg ttttcctgtt aggaggctga caaatcccat catgaagagt
     3661 gcgactactc cgtagtaggc taattttaga agtgcgtaac agacaactgc cagccagtaa
     3721 gaagcacttg gtacataatg ctctgctaac agttgcacaa gatagaataa tcctcctact
     3781 cctgtaaata ggagaactgg aaaagtaatc aaccaaatta gacggaacaa tgaaccgatc
     3841 tgctttctgc ctctggtctt ctcccaagaa taagcgatag actccttcac acccctgtgt
     3901 tcaaagtaaa tgttcggcag agcgtacatg aagcgtgccg caagccagaa gaacaagagg
     3961 aggaaaatca aaatcaatat tcctagccag attgtgttgg ataggtaatc tacgataaat
     4021 tggggcacta cgattttatt aaaataatag atatttaata ctcggcgtaa aaatgggaga
     4081 agaataactg agtaaaagag tagaaataaa acctttggta ggctactgtg acggataatc
     4141 gccaccatac tatctcgtac atcccgtaaa tagtcgggga ggtgcttgac tttttcatcc
     4201 aacagttgcc aaacccctgt gaacaataga gctaattcta gataggcaat ggcaatgttg
     4261 acaaaaaaga ggagaataaa caagcctgtc acgagcagat tttcagcaaa caccgacaac
     4321 aaattcgtcg gtgaaaggta ggcatgccct gtcatagcta agagcccctc agaaatccat
     4381 gaattgagcg gtatccaaac aaattccata aaggtaaaca gggtaaagaa gagcaagaga
     4441 atcttgtcta ggttgtaata tattttttga aattcacgtt gtatcttttt catgattcta
     4501 gtgtcccata ttcttgcaag aattgcaagc cttcatcaaa tatgctagac tagaaaaata
     4561 aaggaggttc tcatgattag aattgcaccg atgactgaag attatttgca tacactttgg
     4621 aagattggct atcgtgaagc cgcacctcgc tggaaggaat ttgcagctcc ctattttgat
     4681 gactaccagg cctgcgccac ttttgaagat tttcaagcac ttgaactcta ccagtggtta
     4741 caaaaagatt gtgtcagagc aatcctagtg gatcagctgc ctatcggatt tgtgagctat
     4801 tactgggagt gccagccaac acgctggctc gaaatcggga ttgaaatcta tgacgataag
     4861 gtctggggaa aaggatatgg cacagctgct atccaactct ggattgatac cattttccag
     4921 caatttacag aattagaaca catcgggctc actacctggt ctggcaataa gggcatgatg
     4981 agaaccgctg aaaaattagg catgaccaaa gaagcccata tccgcaaagt tcgatactgg
     5041 caagggcatt atcatgattc tatcaaatat ggaattttaa gagaagaatg ggtagaacaa
     5101 aagaaaaaat caagttccta agagcttgat tttttactta tctatcttcc aaataaacgt
     5161 gcaaagaagc ctttagcttg cacttcttct gcctgtgctt taacttcgtc caattccaac
     5221 ttgagaactt ccttatcggc catagctttt agtgtcaatt gctgttgctg atccaactgt
     5281 ttatccttct cagcaatctg tacatcttta acctttagtt gttcatcttt ttcggcaagt
     5341 tgcttatctt tggcttttaa ttgactatat aagcggatga tttcagcatt tttctcgtca
     5401 actaaaattt ccatcaattc acgatgcttg acttcatcgc taatcggctc atcttcaaaa
     5461 atggttgttt tgtagatctc ctccaactta atcaagccag aacggtttac aacggtcaca
     5521 cccttgtcat ttttatctac aaattcttca ggaagtgatc tgacacgatt attcatagcc
     5581 tgccggctca ctcctaaaat ttcagctaac tcactgactg ttttttcgat tcccataata
     5641 tcctcggtac ttacatttct catagtttgt atcattaaaa tattaccaca gaaccctatc
     5701 cctgtcaaat ctaggggaat tttcttacca agcattagtg aataatcaca aacctcaaaa
     5761 gcatcttatc tcaatgcaat gttatgtaga aacgaaatat acattctatc tgataaaacc
     5821 atttttagca ctcacttgct tgaaccaata tccagatttt ttgacagtac gcctctgagt
     5881 ttggagatca agcgagacca aaccgtatcg gtttttatag gcatttaacc aagaccagca
     5941 gtcaataaag gtccagacca tatagccctt acagttcgcc ccttcatcaa tacctcgatg
     6001 gagctgaatc aaatggtctt ccatgaagtt aatccgataa tcatcctgaa tcatgccatt
     6061 ttcttcaaaa acatcctctc cttcaactcc catgccattc tcagttacca accattcgat
     6121 attcccatag ttttcctgga tgtttttggc aatgtcgtac agcccttctt cgtaaatttc
     6181 ccaggctcta tggggattga ttttcttgtc aggcttatca taaggctgga aatattggga
     6241 taggaaagta ggttctccgt tccatccatc ttctgaagga gcttgtacgc gcagggggct
     6301 ggtaataatt cacccccagg aagtcaaccc tattttcttt gataatggtc aattcctcct
     6361 ctgtggtttc tggacgtaaa ccgtgttttt caagaatgtc aaccagctcc ctcgggtatt
     6421 gtcccaaaac ggatggatcc aaaaagcttc tcgtttgaaa tagctcagca atctgtgctg
     6481 cctgtaaatc tactggactc tgacttcttg gataggcagg agtaagattg agaacaatgg
     6541 aaattttgtg gtctgggtgt agttggtgac aggcccgaac agccaaagaa ctagccagtt
     6601 gagtatgata cgctacgacc acagctgcct ttgcatccgc cttgcaagga taatgatagc
     6661 ttcctagata gccacattct actggaacaa tgggttcatt aaaggtaatc caatgatcca
     6721 ccagatctcc aaagagactg aaacactggc gagcataagc ctcataggcc cagacaattt
     6781 ccccattttc ccagccccct ttttcctgta agacataagg caaatcgaaa tgataaagat
     6841 tgaccaaaag ctttatccca cgctctcgaa tacctgcaaa tacagcccta taaaactcca
     6901 cagcttgcgg attgacctcg cctatccctt ccggaatcag acgtgaccac tgtatagagg
     6961 tgcgaaaaat actataacct gtttccacca acaaatccaa atctgactgg aaatgttcat
     7021 agaaagtcga tgtttgttcc ggtcctattt gctggtgaaa gagctcaggt tcctgactgt
     7081 accagtaatc ccagttattc cacccctttc catcaccagc tacgcttcct tcactttgag
     7141 gacctgaagt ggaggagccc cacaaaaaac cttctggaaa acgatactct ctttgctcag
     7201 ttaccattct tctacctact ttctttcatg ctacagacgg agttgttaga aaccaggtct
     7261 caaaaaagcc cgcaaggcat cacaattctc aaaacgttaa tatccaatta tatcaacgtt
     7321 tcagagcact ttgcgaagat ttcgcaaggt gcttttttgt tgaaaaaagc gccttggtaa
     7381 tccttttggt aatctgttta gacctagttt tgagagtggt taagctagaa tgactattta
     7441 gctttattga ggtaatccgc gaacttttta atagattgtt tttctcctga ttttgtagaa
     7501 tgactataag tatctaaagt catctggctg ctagcatgac ctagacgttt agcggtattt
     7561 acagggtcaa ttcccatttc aatcatcaag tttgcgtgag tgtgtctaaa accatgtggg
     7621 ctaatctttt taagatgatg atttcgtata atacgtttta gaatattatt gatatagtca
     7681 gcgtgtaagg gttctatctc accatctcgg ttgcagtatg taaacataaa ttctttgtcg
     7741 agagaaagag gttgtactga taattgccct ttttctttgc tagtttcttg tttccatttg
     7801 ttgaggatag aacaagtttc gtcatcaaga tagatagagc ggactgagaa agtatttttt
     7861 gttccttttt caatggcatg accatctatt cttcctaaac ttttgctaac tgttaatagt
     7921 tgattttcag aatcaaaatc ttcccacttg agagcatata gttctccctt gcgtaatccg
     7981 ctataagcta ataatcggaa tagcgcataa tgtttatagg gttcattttc caaaataatt
     8041 gtcaaaaatt gatgcaattc gttaactgtc cagtaattct cactgacggt ttttttgagt
     8101 ttcggcataa taaccttact cataggattg cgctcaacat agttcatgtt gagcgcaaat
     8161 tcaaatattt tagaggtgta agatttgatt tgcttcatat ttttaaaagt tttagctttg
     8221 ttagttatga aagtttggca gtctagagga ctgatttttg aaattttctt ttctccaaaa
     8281 acaggaagaa tatgtaggcg atagaggtcg gcagtttttg ctgcagtagt tgcttcaacg
     8341 gtatctttgt atgcttcata ccacttttga tatagttctt ggtaagtata ggttgtttga
     8401 gtagtaagat gaacaaaagt cccactattc aactgatgat taacagcagt ttcgtgcgcc
     8461 ttagcagcct tacgagtttt aaagcctcgc ttagtgtaat attttttctt accatagtca
     8521 tttaggccaa ggtaaacttt aacaaaatag gtgactcctt ttgtagattg gtacttgtgt
     8581 acagacataa cagttctccg tttctaagtt ttcggagctt atgtaattga gaaactgttc
     8641 gtttattttt tgtgtcggtc ttcttgtcta attcggtatt cttcagtctg aatccaggtt
     8701 tgccaaattt ctgactctcg ttttaaacta ttcatgtgtt tagtgaaagt tttcatgaat
     8761 tcttcctcat gattgatcaa gtagcgcgct aaaatgcgcc taaaatcgtc tgagaagccg
     8821 tcatttaagg gcatagacag atggatttct cctagttcaa ttcctttctt cagcttttcc
     8881 atatcgcttt taaaaggtgt tacagaacta atctcggaaa ttttaaaact gtccgtaata
     8941 atgttcatag ttgacggtgc ttgtttgtta tcatcaagtc ctaagatgta agccatggat
     9001 acgccgaaat agtcagctaa tttttgagca tttccttttt tcggttgacg aatgccttga
     9061 ttgtatcctg ataaggttgg ataagcgata tttgtagcag aactaatctc agtcagtttt
     9121 ttgccgctct cttctatcag ttttgcaata tgattggtca taaatacctc cttgagagta
     9181 ttataacaca tttaattctt attgaataaa aatagttgac aaaaattcca aaagaatata
     9241 taattcaaaa tgaataacaa ataaattcgg agtttattta attgagaaat ggaaacgagg
     9301 taataatatg tctgaagaag cgactttagt tgttcaactt cccaaggttt tggaaaaaca
     9361 attaagggca cattatgatg aaatggtttc tacagctatt gaaagagcgc tagaggataa
     9421 agaattatat aaacctatgg ttcggatggc agccctttct agatggcttg atgtgtcaac
     9481 gacaaccctt caaaagtgga cacgagaagg aatgcctaca atggttattg atggtgtgac
     9541 tctttacgat aaacgtgcgg ttacacgttg gttgaagcaa tatgaaagat gaggtaaagg
     9601 atggctaata gacgaatgtt gagtaagacg gtggtgcaga cacaacgatt tttacgccta
     9661 ccacttgaag cgcaggcact ttactgtcac ttggtagtca atgcagatga tgatggtatt
     9721 gttgaagcct ttcctgttat gcgaatgatc aacgctaatg aggacagttt gaatttatta
     9781 acccttaagc agtatttatt cttgttaaat aatgaaatgg tttactttat caaggatttc
     9841 tttgagcaga ataccatcaa ggcggaccgt tatacaccga gtagtcatcg tcaacttctt
     9901 atagattatc tgaaggataa tcagttgttg gagtgtctaa atctaggacc acgcttgatt
     9961 aattatcttc aaaatactcg ggtccaagtt ggaaccagtc tggatccaga tgggaaccgc
    10021 aatataatta aagataagat aagtaaagat aggataggta aagataagtt aagtcaagat
    10081 aagataggat tagataatga tccattctca ttacgactga ctgatgacat ctggttaatg
    10141 gaaacactat ggcatcgaga attaacccga caggaaaaag tattactaga aggattaaca
    10201 gttagtgatt ctttattgca gttagctatt cacaaaacag ctgagcttga gccaaaaaaa
    10261 caaaacatga gttatgtcaa agggatacta atgaattggg aagctagagg gtttataact
    10321 gttgaccaag tgatagcgtc tgaaaaagac taccgtccat tagaaatttc aaatgtggaa
    10381 gtatctgaag atttcatagc agctatggat atatggaaag attagaagga taactagtga
    10441 tgacagtata ttatcaatta gaaaatatgt tagtggctgg atttaagtca caagaggagt
    10501 acgaccgcta tcaggctctc aagagagact atgaagggga gtcagaggac tacagctttt
    10561 ctattagaga aatcaccaat cagattgaaa tcatttgtaa gacctcagaa aatgattttc
    10621 ctgatttaga agaaggaata ttagctgact atatggaatt agtagctcag ttaaaacgct
    10681 atgatactaa gcaggcagat aagtatttaa ggatggttcg tcatggttga acgtgtcaga
    10741 aatattttaa agaagtttta tgtgacggaa ttgcagaatg acgtccttaa tcggttagta
    10801 aacgatacag gattagggtc attttctaat tatgctcgtc ggatgctctt taaggaaacg
    10861 tcacttttta ttcaatttga tgaatcacag tttgaggaat tgatttattc tttaagacgt
    10921 gtcgaaaata atttaaggca gttatcaagt attgcagaac agtctcaaaa tattcaagct
    10981 tacagagcta tagagtatag tagaaggcta gtttcaaatt atgaaaagca gttaactcat
    11041 tactacaagc agaagaaaag aaaattatta tctaagggag cttaatggtt aaaaggagaa
    11101 ttagaaacgt tattaaacaa gtctttttat ctgaagaaga aaatcaaaaa ttgttaaatc
    11161 gaatgaagca agatggcttc tcaaactttt ctatttttgc tagaaagcaa ttattaaaac
    11221 ctgattttga aacatggcta gtaagctttc cggaatatca gactctgacg aatagattgc
    11281 tatttattgg cagaacaatt aattctattg ccaagagtgc gacccagttt gggaaaatct
    11341 ctccgcaaga cttgatggaa ttaggtcagt tgatggagga acttgtagaa catgtagaga
    11401 agcaaatcag ggaagataag caacgagtag ctaagaagtg atagtcattc agtcattttg
    11461 agagaatagt tttaaaataa aatcgagtag ggactaatct ctagcccctc tcacaccacc
    11521 gtacgtgccg ttcggcatac ggcggttcaa ttaacttttg acgcatgtcg ttctaagtag
    11581 taatccaagc atgaaaccag tcctcttttg acgagaactg gttttgatat agctcgtttt
    11641 aagacggact tttgtgccac cagttgatag tggtctcccc atccagatac tttatctgct
    11701 atccatttcg gaactcctaa tttaagaagt ccccaaagtc gcttagattt cttcttccat
    11761 tgtttccaga taataaccct aactcttgtc ctcaaccttt catcaatgct taacatggtt
    11821 gacttcatat tcgtcaaagc aaaatagttt atccagcctc ggataagcca gttgagtttc
    11881 tcaatacggc tatctaagtc aatgctccac ttacgggttg ttagtttctt gagttttctc
    11941 ttgaacccct gtatactgtc ttggtgtggg cgacttttcc aaccttcaga tgacttccag
    12001 aacccaaagc caagatactt cagtttacct ggtctcacaa tcttggtctt agtcatattg
    12061 actttcagtc cgagacgttt ttcaatgaag cgactaactg aatacttaac acgtttagct
    12121 gccgcctcac tcccaacagc tatgacacaa tcatctgcgt aacggacgaa gtggaggcct
    12181 ctgttttcaa gttctttgtc taactcattg agcatgatgt gggataagag cggtgataga
    12241 ttcccacctt gtggagtacc cactagcctt ttatggcatt gtccgttgat gactacgcct
    12301 gaatgaaggt acttgcgaat cagagattct gtatcaccat cttgaatcat gttatgaaca
    12361 agtgacatca gtctatcttg aggaactgta tcgaagaatt tttctaagtc aatatccaca
    12421 atccactcat agccatcatt aaggtactct agtagcttga tgatggcttt ttcacatgac
    12481 cgatttggtc ggaacccata actcgtttca gagaaataag gctcacagat gggactaaga
    12541 acttgaacaa tagcttgttg gattatccta tccatcactg tcggaattcc aagctgacgg
    12601 atgcctccat tgggtttagg gatttctact cgcaggactg gttgaggttt gtatttcctt
    12661 tgttttatca atctcttggt tgatcgccag tgttggcgga gatagtcatc tatgtcatca
    12721 attgtgacgc catcaactcc agctgagcct ttgttagctt tcactcgatt gtaggcttca
    12781 agcatattgg accgagatag gatattatct agtaattgtg acatgtgcgt attcctttct
    12841 tgtttcggtg tctgagggac gtcttatatt agtgaacttt cctctagtca atacgtgacc
    12901 gcacccagtt ctatcagacc gctaatttta cagacacaag tgaagtaggt atacttcggt
    12961 taattgttca gcccttcgct ccacttccat tacagaggtt tcttcactac tatggcctcg
    13021 gctgacttct catgattcgt tgttactacg tctttgacgc tcatgagacc tcacgggata
    13081 agtcgtacat ctttcctcgt ttactctcga gatttacgca tataggttac gactacctat
    13141 ttggacttta gagttgtgtg cctcctcatc ccctatatac gccttgatat cacgtttctg
    13201 ttcgtagagc cacgatttcg ctatcccttc ctctgacgat cttggtttct agcgtcagac
    13261 ttaaaacggt ctctccccag accgttttaa tccgctacct cacgatagtc aggcttggga
    13321 gtcgctattg ggttcgccgg tagcgggtgc ccacagagga cttccacctc agatgtacaa
    13381 catgcccgtc gtacacagca aaaacgctga ctaccatcag cgttttttag catgtaattg
    13441 taaaaaatcc ttgtacggac aatgtgcggc accgtataaa gtacgggaaa tcactaaaaa
    13501 tggagttagg tctttgcgag ctcagatagt gtatacacac tatcgcaaaa aacaaacatc
    13561 agtaaaggtc agactgacac tttttgactg atgtttctat ttttattttg gggagaatcc
    13621 ccaaacccct agaaaataaa aattttatca aacaaaatat tttaccattt actacattta
    13681 aaataacaga ataatacaaa tgaaatttaa tcttaataat ttaaggtata ctataaccat
    13741 caacagagat gttgaaataa ttcaaaggag gaagctatca tgcttaaatt ttctacaaag
    13801 aaaaccaaaa aggcaggctg tgtgagtgta ggtgcactag tttcagtttc aattggtcac
    13861 tacattaaag aaattaagag tacagtatag cttacttttt gagtgtcgga gaagtacaga
    13921 gaaatgtttg tagtgatggg aattgttaaa aataatgatt gctgaagtat catgtttaat
    13981 tgtttcttat tgcaaaaatt tctatgtata atcaatcgtt taggtaggct aatgttaaat
    14041 tagcctactt tttatttaaa gatgctatga aagaaaataa aaaactccag atattagtac
    14101 agtgtttatt tcaagtaatg atttcaaata tttgcttgat actatatgtc ttattttgtt
    14161 cagttttttt aaaaactcag agtatactgt tatcattaag tacccctaca atagttgctc
    14221 tggtagtaca gccaatattt ttggaaaaag ttttgttcaa aagtaaagtc aatttttcca
    14281 aacatattat tgttgttata tcggccttag taagtatttt gatttgttcc tttgttaata
    14341 tacctgagaa cattatattg attttacatt ttattttgat aggttattct gaagaaatat
    14401 tatatagaat gattatctta gataggatga agtcttctta taacatttta gaatcgatag
    14461 tgattactgc gcttatcttt gcttttttag gtcatatatc ggaacctatt ttagataatc
    14521 taatgataag atttcctttg ggaatatttt tagcatttat cagaataaga tttaataatg
    14581 ttggaattcc aacgattatc catgctttat ataatgtttt agtaactttt tagaagaggt
    14641 ttttatgaaa attaaattgg gttcaatatt tttattttgc ttacttattt tgacattcat
    14701 caattataat tttatttcaa cttataattt gtttacgttt tatatattgt tgatgcttct
    14761 tccttcaatt ttgtattcaa attgttgtgt tattttctta aggcacttca ataaaggtac
    14821 aacagtatat tatttattat caaccattgt acatttttta gtgaattggg tgataattca
    14881 ccgattgttg acaaaggaag tatttaatag ccttgtcgaa aatacaatga aaattaatcc
    14941 tagtgtaggt aataatattt cattagactt gagtttatcg tcattttttg ttattagcct
    15001 atttgtattt ttattaatgc ttattgaaaa ttattttata agaaaagttg aggagaaata
    15061 tgtttctaaa tgataatatt tcagtaatga aaacagatga aatgtatcta ctattcaatt
    15121 ttagcaacgg gatagttgct ggggtagata gtcaagcaat tgaatttcta aaacgagtaa
    15181 aaactcatca attaaattgt aacgatttaa cagatgaaga aaaagaattt tgtacctatc
    15241 ttgtagaaaa tgatttcatt tctgatgtag aatttaagaa tgagcaaccc ccagtgactg
    15301 cctatttaca tttaactaat aagtgtaatt tgcattgtgt aggttgttat tctttcgatg
    15361 agcgaagaaa tagatgtgaa gatctatcca cagaagagat attaaatgca attgaccaac
    15421 tttctgatgt cggaattcaa aacctagtat tctctggagg agaaccttta ttaagaaaaa
    15481 atattattca aatagtggaa tatgctaaaa tagttgctaa aattccaaat gtaatattaa
    15541 taactaatgg aactattttt aataaaaaaa tactttcaga actatcaaaa tttgtagata
    15601 ctgtttctgt ttcattagat acatactgtt cggatagtga accatatctc agagataaag
    15661 gaatatttga caagattatg cggtcaattt cttggttaaa agaatcggga aataatgtca
    15721 atattattcc aacacttcat catttgaatt caagtaatct ccttgagtat gttaaacttg
    15781 caaaggaaat aggaacaacg attagtttca gtattctcag tgcgtgtttt gaaggcgagt
    15841 taatgtcata tcttcctaat gacactgatt taatcaatat cagtgaattt atgattgaat
    15901 ctgatgcacc aatcaatgat agtgctctag aggataaaac tttagtagct aggaattact
    15961 gtggagctgg tacctcaatg attgccatag gtactcaagg taatgtttat ccgtgtcaca
    16021 tgttgatgta tgatgagtac tgtataggaa atataaagta cgattcaata aagaatatga
    16081 gagagaaatc tgctaatgca aggttattca aatcattgaa tgtagataaa atagaggggt
    16141 gttcacaatg tcaattccga tatctatgtg gaggaggctg tagagctcgt tcaaaattga
    16201 aatttgacgt agtagataaa atagatccat actgtcgatt atttaaaaca tactataata
    16261 aagttgcgac ggaaattatt tctaattagg gagaatgtct atgaaaagga tcttaaggga
    16321 agaacgaaat aaaatagtag tatttattat ctctacgttt atattgggat tagttgaagt
    16381 atcgtttgct acaataattc aaaaattcat tgattcccta gagtcatcaa atattacaaa
    16441 gtttaaatta gcaatttttt cctttgtagt ttttcttata gtgaattata tcttgaatct
    16501 ttttgtaata tggattcaat cagtacttac taagaatatt catctgtatt taaaatcaaa
    16561 tttggctaaa tattttttat cgcttggtta ttgtgatttt tctgaaacaa ctacaggtga
    16621 aaagttgaat tgctttgatt ataaaatatc gctaattgaa cagtattatt tagatagtat
    16681 ttttgcaatg atacaagcaa ctttggtgtt gatgattgcg actgtttatt tatatcaaat
    16741 tagcccgttg atttgcgtga ttgtatttac tttaggaatt gtatctatgc tgattccctt
    16801 gttacttggt aaaaaattaa ataatttagt ttctgaaaat gcagatctaa gcggagagta
    16861 cctacatttt ataaaagaga tgtttcaagg aatactggcg gtcaagacct ttcagaagga
    16921 gcggacgttt tttgagaaat ttgaagagaa gttatttaga tttgaaaaga atgctcaatt
    16981 gttaaatgta aaaaatggtg agtataatca aatcagtggc ttttttcagt atgtaatcat
    17041 tatttcttgt ttatgctttg gtggattttt agtgttgaat caaggattga cactaggaaa
    17101 gatgattgca gttacacaaa tttcaaattt gatagttggt cccattcaac agattggctc
    17161 ttcagttatt gatattttgg ctagtaagga tataacgaaa gaaatgtcag atattttttc
    17221 aaaacaatcg attgttttag ataaaaaagt atatcaaaaa ggttttgata atataagggt
    17281 ttctactaat agtgtcacaa aagataataa aaagctcttg ggtggtgtta atctatctct
    17341 tgaatatggt aaaaaatatt gtattattgg tccaaatggt agcggtaaaa ctacatttgg
    17401 acgcttaatg ttaaatttgt taagtaatgt tgaagaacga gtatttatag atggagaagc
    17461 tagtaattta gaagatgtcg ccaaattgat ttcatatgta ccgcaaaata gtattgcttt
    17521 taaaggtact ttgaaagata atattacatt gtttgaacgc tatgatgata aaactattga
    17581 taaggtaatt agagacgtta atttaaacaa taaaagtaaa gagattcaaa tgatttcgga
    17641 aaacggggac aatatttctg gtggagaatt gagaaaagtc aatatagcta gagcgttttt
    17701 agaggataga ccaatattac tagttgatga atttgagtct gctctagatg aagaatcgtc
    17761 aaagctaata gctgataaat ttttaagttc agaaaaaacg gtcataatga tttctcacaa
    17821 tttagatcag tcatttttac aaaggtttga ttccataatt tttatggaaa atggggaagt
    17881 tactgcaatg gaaaaatacg aagtattact aaaaaattgt gacaacttct ataaatattt
    17941 aattcatact gcacctggtg gaggtaatat tcatgagtat aatactacga gcgtgtaatt
    18001 taagtaaatc gtttgattca caagaggtgt taactaatat caattttgac atccaatcag
    18061 gagatatagt tggcttgatt gggaaaaatg gctgtgggaa gactactcta atgaagatga
    18121 tattgggtct cacatcacga acggaaggtg atattcaatt tgaaggtgat tcgaattatc
    18181 atactaacag aaaggcatta aataaaattg gtttcctcct agattgtagg ttgtttgaag
    18241 atttctcagc atatgataat ttgaaattat tctctatgta ttcttcaaca gttaatgaag
    18301 aaaatttaga tgatcgaatt gataggttgt taaaatttgt tggtttagat aacagtaaaa
    18361 agctagtaaa atcttattcg tttggtatga agcagagatt gggtttagcg ctagcattat
    18421 tagatgatcc tgaattcttg attttagatg aaccatttgt tggattggat cctacggggg
    18481 ttagagtcct tcttgattat attgtaaagt tacgtcaaga aaagggtgtt acgattttaa
    18541 tctcatcaca tcagttacat gaaattgaag aaatttgtga ttattttttc ttgataaatg
    18601 ataagagaat tgaaacacat tctaaattag gagaaaatca aatagttatt actttaggga
    18661 atttagtgcc agagttggaa catagtttaa gagatatggt tgatatgaaa gaagatcaga
    18721 taactttacc agaagatatg tcactactga attcaacatt aaagcttatt tacaaatata
    18781 acggtgaaat aaatgatatc acaataaacg attctacaga agaattattt aaggggtgac
    18841 aaaatgaaat caatcatttt agcagaacta tataaattac taaaacgaaa aaattttatt
    18901 atattagcgg tgctatcttt gtggtcattg atttacggat tgggtgcttt tttacattgg
    18961 gattttgtga aaattggtgc taagttagat ttagttacct ttattacaac gatgtggacg
    19021 tttttattga tattaactat tccattatta ttaatcttgt acacttcttc ctttatctta
    19081 ggaggagaaa tccaaggagg gcaaatttta ctagaagtaa atcgagtgtc tagtaagaaa
    19141 acgttaatta tttctaagta tattgttgtc attttatcaa ttttattttt atatgtactg
    19201 aactgtttat catcactaat cacatatatt tgttttttat ctcgaagtga gtttggttat
    19261 gtgaattttt gggagttgca tcctacaaat atgcaatcaa taataattag tttggtaaat
    19321 ttaggtttta ttattttatt ggctagtgta gccttttact tatcattata tgtttcggca
    19381 atagtagctg catttgcaag tttaggtgtt tactttttat gtcaattatt gtcatatgtt
    19441 actattattt caaaatttgt tccaggatat ttttttgttg tagctaatta tcatttttca
    19501 tttggattat ctatgatcca tttgttgagc tatttctcaa tctgtatatt attattggta
    19561 attaccataa aaggatttga aaagaaagca ttatgagtgc tataatattc caaaaaggga
    19621 tttatagaag cggatgtata gaatagcaat ttgtgatgat gatagtttac aagttaataa
    19681 tttagaaaat cagatttcga gatattttga cgagtttaac gttcaatatg agattgacgg
    19741 ttactataaa ggagatcgtc tagtgaaatc aatgaaagaa caaatggaca attatcaatt
    19801 gatattttta gatattgaaa tggagggtat caatggtatt gaaactgcaa aattattgag
    19861 gaatttggat aaaagtttca tcctaatcta tgttacaagt tatgagcaat atgccttaga
    19921 gagttttgag gtatcaccat ttcgttattt aataaaaccg gtatctattg agaaactgga
    19981 agctgttctt agggatgttc ttgtggaatt aaccgcgaag cagaaatttc tcttttatca
    20041 agtaggtatg gagcattttc aggtaaactt agataatatc gtatgcttgt actctgaatt
    20101 tggtagaaaa attcatcttg aattagttga tggtggtaat gtgtcgtttt atgggaaaat
    20161 atcggaaatt gaagaaagac tgcctcaaac ccattttatc agagtcaata gcggcactat
    20221 gattaacttg aatcatgtta catcacttaa tcgaagtgat gtaacgatga taaacagtga
    20281 ttgtattacc attagtagaa gtcgtaagaa aatagtaata tcagcttaca gtcagtttat
    20341 tgagaggagc tttgggttat gattaactta tttaattcaa ttggcctcct ttctcttttt
    20401 ctgtttcaaa gctttattat ttggatttat ttacagcatt tttttgttat tagaattcct
    20461 aaaggtaaat tctattgctt tattttaggt gtttatttca ttggtagaac gtctataaca
    20521 gttaatcacg ataccattat tgattttaaa tttttttctt tgtttttcac gttcttgata
    20581 ctgttctttt ttttatcagg ggatgtaatg aaaaaaatat ttcattactt attcttgatt
    20641 ttttggtttt tgattcaaga agatattaca atgttactgt tgaatcatgc gcaacagacg
    20701 agagcgatta ttatttttgt cgttcaattt ttattgatgc tattaacgct tttatttata
    20761 cttggattaa agcattttaa gatagataat ttattaggtt tgaataaaat agagtacggt
    20821 attttatcag tcactccttt gttgtccatc attctattgc tacataaagt ttcactacct
    20881 atagataaag ttcttatatt tgatagctat ttattattaa taaatattgt aataattgta
    20941 ctttataatt atttgagcga aaaaaattat aacattatca aaaacaaaat agcttttgac
    21001 gaaaatagta tgacttgtga aattataaaa caagaagaag aattggcaac tcttagacat
    21061 gatttaaaaa atatagtttc ctcaatagat ttttacgcag atagtagtga ttataacaat
    21121 atcaaggtga taaccagaga gatattggga caagaagtat ttaatcgtaa aatcacagga
    21181 tgtataccta ttgatgctgt gctaaatcaa aaaaaatcta aaatgaaaaa agaaggtatt
    21241 ccatataatt tagatttgca aattccttac aacttagatt taagtactat tgccattgat
    21301 tcgtgtgcca ttcttggtaa tattcttgat aacgctatag aagaagttga acgtaacggc
    21361 ttagaagaat cgattgaaat agcgttacgt tttaagaata ataaattagt tttcaaagtt
    21421 gccaatccaa tcaaagattc taatgtagat gttaattacg atggaatgaa gtctataaaa
    21481 tctcctaaca ggcaaggtat agggataaaa agtagttatg atcgggctat gaaattgaag
    21541 ggacatttaa atattactgt ccagtcaaat caatttattg tactggttgt gattccttta
    21601 aggatgaact gagtctttat tgggaaatct atattcaaac tctgctaata ggtagagttc
    21661 tttttctaag gtgtctcaca gagagtttac tgatacgata ataggttata ctaaactaga
    21721 cagattttat aaagtgttct acactaaaga aaaacaggag atacaaatgt ctagaaaacc
    21781 acgtcgttat ttcacagatg actttaaaca acaaatcgtt gatctttaca aggcaggtag
    21841 gaaacgcagt gaactcatca aagaatatga tttaacgcct tcaacttttg ataagtgggt
    21901 caagcaggcg cgaacgacgg gttcttttaa aactgttgat aatctgacag atgaacaacg
    21961 tgagctgatt gcactcagaa aacgaactaa agaacttgag atgcaacatt ggaagatatt
    22021 gatgaaaaac tatcgtacag gatttcaaaa gtcactttga tttatcagag ccctccacag
    22081 aggaaattct caaagctaga cgttttttgg tcagtggtaa attgactaaa gctgcgcttt
    22141 tactttttgg gaagtgtccg tcagcttttc cctcaagcac gtgttcgatc caacgatttg
    22201 aggggacaat gattgcagtc aggatactat tagtaataag aatacagtag aatacaagtg
    22261 ttgctagggc accaaattat tcaatatgtt ggataagttt gatttaggtt aaaagtatag
    22321 tcaaatgaat taactttgag acaaagaact gagacgcagg cagcacttga gtagggcaag
    22381 gcgaaagtga cgatgtctca accttaattt aaaagactat atctagatga gaaaggggaa
    22441 gaggaagaga tgatttttat tagttataat cacggagatc aagatgtagt agatatgatt
    22501 gctaaacgat tagagattga gtttggacga gataatattt tctatgataa atggtctatg
    22561 taaccaggag atagtattat aggcaaaatg gatgaaggac ttgaaaaagt tacaacattt
    22621 tttattttct atcatcaaat agcttgaata gcaatatggt caaaaaagaa tggcaagcgg
    22681 ctctaactaa gtcgctctat gagggagtta aatttgtccc aataaaggtt tctgattgca
    22741 agataccagc tattataaca gatgtatact atattgattt atttggtcaa ggattagata
    22801 atactattga acaaatgcga agtgtgataa gtggcagttc gatatataca cccttacctg
    22861 atatagagaa tttacaagtt tctattaaaa atatatcaac ttatgaggtt gagtttaaaa
    22921 atttttacta cacattttgt ggaaagtgat gcatcttttg gttttgtttg caaaaatcca
    22981 attgactcat atgaagttcg tcccttaaca gaaacgatat atcggagtat gcaaggggaa
    23041 acatataaga aattaagtga tggtagagag aaagcattta aaatgcaaat tggaatgctg
    23101 cagcgttcta ttaccccaag tcaacctttt ataggtaggt tggtctctaa gcaattacca
    23161 ttacagctag aaggtatttt gcatataaag tcatttgaac tggtaaattt tagggaatta
    23221 aagataaata ttgagcagtg attagtatct aaaataactc gagtcaggga ataaaaaaac
    23281 aagtcattct atgacgactt gcttatccat ttctcaacta cataagctcc gaaaacaaat
    23341 ttttggtaat ctaatggtaa tccattgttt ttaggattag gctataagtg tttaaattta
    23401 atgaaactta cttttgaaag cttgatttaa taagctttta ttgatatttt acttttgcta
    23461 cccaaaacct ggattcacaa tgcagtttcc acaaaattac tttgcttgta catacaggct
    23521 cctgataaaa actaatttcc cttagtttaa gtatactccc ctaacccgtc aacggtcttc
    23581 cgcaatctca aggaaaaact ccacaaatca tagcaaagac tcaaactact ttcttgatga
    23641 aaatatgata caataaaggc atgaatacag tagataccat tattatcgga gcggggcctg
    23701 ctggcatgat ggccgctatt tcttccagct tttacggcaa gaagaccctg ctcctcgaaa
    23761 aaaacaagcg tttgggcaag aaactgtctg gcacaggtgg cggacgctgc aatgtgacca
    23821 acaacggcac actggaagac ctgctggctg gcattcctgg caatggccgc tttctttaca
    23881 gtgtcttttc ccagtttgac aaccacgaca tcatgaattt ctttcaggaa aatggggtca
    23941 agctcaaggt ggaggaccac ggtcgtgtct ttccgaccac cgatcgttcc cagaccatta
    24001 tcaagtgcct agagatgaag atgctggaaa atggtgtgga catccgtaca gggactgagg
    24061 tggtttccgt ccgcaagatg gatgaccttt tccatgtcaa gaccagcgag gaagtcttta
    24121 cggcacctca gctcattgtc accaccggcg gcaagaccta tccttccact ggctcgactg
    24181 gctttggtca cgacattgcc cgccacttca agctggaagt cacggagatt gaggctgctg
    24241 aaagccccgt cctgaccgac ttcccccaca agaagctcca aggcatttcc ctggacgacg
    24301 tcaccctgac ctacggcaag cacgtcatca cccacgacct gctctttacc cactttggcc
    24361 tgtcgggacc cgctgctctt cgcttgtcca gttttgtcaa aggcggcgaa accgcatttc
    24421 tcgatgtatt gccgacccat tctgaccaag acctgtttga gcatttagaa atcaacaggg
    24481 aaaaatccgt caagaatgcc ctccgagaac tcatgccaga ccgcttagcc gacttttttg
    24541 ctgaaaacta cgactgcaag gtcaaacaag tttcccaaaa agacctgacc aatctagtca
    24601 cccttctcaa agccctgcca attaagatta caggcaagat gtccctggcc aagtcctttg
    24661 tgaccagggg aggcgttgac ctcaaggaaa tcaatccaaa aacattagag agcaagaagg
    24721 tcccaggcct ccactttgca ggtgaggtct tggacatcaa cgcccacacc ggtggcttta
    24781 acattaccta ctgccttgcg actggctggg tggttggtag ttcctactct tcataaatca
    24841 aactcagacg ttgttgactt gatttgatga gtgtaaagct ccagtggagc tttacagcct
    24901 gctacacgaa aataaaaggt acagcgttct atctacatct aatggcacga actttgttcc
    24961 cctatctcca acctccaaag gttccccgaa cctttggagc tagtctgagt tcgattttct
    25021 ttgagtagta cactactaat tatttaatga ctgaaaaatt caagcaaaat ctgcttgaat
    25081 ttttttgttt tttgttgtat gataaaacta taacaatata acaaaaagga gattgctatg
    25141 caactgaaac tttcttctat ttctaaaaaa tttgaccaca agattattct ggaggatgcc
    25201 aattttcaat ttgaacaagg caagatttac ggcttgctag gacggaacgg ggctggtaag
    25261 acaaccttgt ttaactgcat tgcccgtaat ctgaccttgg ataatggctc gattcagttt
    25321 gtgaaaggcg aagaaactct tgactacgat aatacggaca taggttttac ccagacctat
    25381 ccgcaactgc ctgcctttat gacggccttt gaatttgtcc gtttctatat ggacattcac
    25441 aaggataaac tcaaatctcc tcgcagtcca gaagaatggc tgaacttggt cggtatcgga
    25501 gaagaagacc aacatcgcct gctcaaggac ttttcccacg gtatgcagaa caaggtccag
    25561 ttgcttctgt ctttgattgt ccagccacct gtccttctct tagatgagcc gctaacgtcc
    25621 tttgaccctg tggctgccca cgagttcaaa caactaattc gggaagctaa gaaggattcc
    25681 gtcattatat tttccaccca tatcttacaa ctggctcagg acctctgcga cgagattgtt
    25741 ttgctccatc accaagaatt gcaagcagtt ccgagcgaca aactccatga tcctgacttc
    25801 gaacaggaag tagttgcctt gctgacagca gattaggaga aacctatgaa aacaatatta
    25861 cgttattctt ggtatcagga aaaatataac ctccatcggt cagtcaatgg attcttctac
    25921 tatcttcgga aactgccgct tgtgggaaag tgcattccag agagtatttt caagtcttac
    25981 agctttaagt ctagtctctt tttggtcctg cacatcttat ccatccccag tcgctttctg
    26041 gtcaagggct tctggttgct ctttcatttc taccatgctt ctttctggat gaatatgcta
    26101 gtcaatgggg agctgacctt ttggaaaatt ctcccaaaca cttggctcct tggctttatg
    26161 ttctggcttt tgtttgtcgg actaagctat cgtttgggga agagcttaga ccctgttatc
    26221 tctaaagcaa atcgagaatt tatgcagaat ttcagcctgt cacaatccag ctttcttcaa
    26281 agccaacttt ttatcgagcc tatcctgacc actatttact acctgccagc cttaaccatt
    26341 ttttgcatga taaccaagga gtggttagtt tttcctattg gtttgatgac agtgctggca
    26401 ggtcatctag ctggccatgc cctcaatcgt ctgctctttg aacgtcgtat cttttcccgt
    26461 cgcaattcgt ggcatgcttg gctatggatc gcgtttgttt ttgttttata tgttttggct
    26521 attgtcttcc gtaatcaact ctccttaggc atgctagtcc ctatcctgct tgtccaacca
    26581 tttctgattt ggtttagcta tggttatttg aagcaacaaa ccaaccacct agactatctt
    26641 tcctactgta tggacgagtc cttgcaaatg gataagaaac tttttgagat gaccaaggga
    26701 aatgaataca ctcgccaggg tttgcagatg caggcaaaac tcaatgcgaa aacaggaaag
    26761 gacctgtccc acctgtctgg catgacctac ttaaatgctc tgctatttga ccgctacaaa
    26821 aaagtcctct ataagaaggt gcagaactgg gtcttggcag tagtcgtgat tctggcagcc
    26881 ctggagggga ttcgttactt tttagatccg tttaccttta cggaagccta cttgctccaa
    26941 ttcctaccgc tttccttcat gattatgtac gtagcttcgt cgggcaaggt ggtggctcag
    27001 atggtctttg ttaactgcga tatttccatg cttcactatc ccttctatcg agaagccaag
    27061 actattatcg ctggttttaa ctaccgattt ttgcagacct gcaagctcaa tctcatcttt
    27121 gcgaccagcc tctttttggc tatcatggtg ctggggcggt ttgcctttag tttggaaacc
    27181 attctactaa ctgccctgct cctcatcagt ctgaccgctc tcttttcctt ccacgatctc
    27241 tttatctact atatcctcca gccttttacc aatgacatgg atgtggtcaa tcccctctat
    27301 aaatttctta gtggtgctct ctactgggtg gcctatctca acacccgaat caatgtaggt
    27361 agccacacct atatcctgct ggtttcccta gctatgattg cctatgttag catcggttac
    27421 tggattttat tgaaaaaagc tccgcagact tttagattaa aagcatgaaa aacagcctct
    27481 cgcaagagaa ggctgttttt taggtatgac gtgcatgtcg tatcaaaaaa aatccaaagt
    27541 cgaatgactt tggattttgg gggttcgata gataataact gatacctacc aatcatagcg
    27601 agggaatttc cttgctatga aagagtatca tactactatc ttctaggaaa attgcggctt
    27661 ccaaaagagt tctgaggaag agcttatctt aagcttcttc gtctcttctt ctctttgctg
    27721 caagaagtgc taatccgagt ccaagagcta ctaatccgag tgaagttgct gaatttgctt
    27781 cctcaccagt attcggtaat tgcttagctt tcttctcaac attatttgca tttgtagtaa
    27841 ctgatttaac tttcactcct gctgcagaat tattttcgtc tactgctaca acttcagctt
    27901 ctgctgaatc agaactattt ccaacctcgt ctttagagta tgctgtaaca actgtaccta
    27961 gcttaataac agaacctgga acgattactt gcccagtcac agggtccaca atcaccactg
    28021 tattacttgc tgtccataaa ccatccgtac ctttcgtaac aactacagtt tgcggcttac
    28081 cattgttatc tacgtatgaa acaacaatct caacagcttt ttcagacggt acaatcacaa
    28141 tattaccttt gccatcattt gcgaccgtag gagcagtttt atctgcctgc ttcacaactt
    28201 ctactggtga tagcaaatca atagttccat ctggatattt aattgtcact gaagcatcct
    28261 tagaaacatt aatctctgtt tcttctggga ggttattgtt ctctttaagg aatgccttga
    28321 tacgagcaat ttctttttca gtcaagttat ttggatcaac cactggtgtt actttcgcag
    28381 ctggtttaat tagagctgcc attggaagtt tgattttttc caactcttga tgagttctaa
    28441 caccattgat taaaacaatc gcttcctcaa cagctttatc aatttcagat ttagccactt
    28501 ccttagctgc atctgtcaag tttggattgg ttgcaattgc ttctttagca gcatctgctt
    28561 ctctagcaat cttgttctta gcatcttgtt tagcggcgtc aagaacggct ttggcgatgg
    28621 cgcctacgcc tttgtcctct gccgattgcg cttcgactgg acttgttgaa gcatcaattg
    28681 catctgtcgt aactttagca tcagcatcta ccgctttctt agctgcggct ttctctgcat
    28741 ctgtcaagtt cggattggag gcaatgactg acttagcgga ttctgcttct ttagcaatct
    28801 tgttcttagc atcttgttta gcggcgtcaa
//
LOCUS       DATUCG010000012        28177 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.012, whole genome shotgun sequence.
ACCESSION   DATUCG010000012 DATUCG010000000
VERSION     DATUCG010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28177
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.012"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(<1..475)
                     /locus_tag="VCA20_000448"
     CDS             complement(<1..475)
                     /locus_tag="VCA20_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834310.1"
                     /translation="MKTNLLKTAGLLAGCVRYFFYFTIALYTFSTLAGFRGDSFVIGS
                     WEYTFNKPIDQISVWLILLTSGLILIILAVLSHIAHLLQKLCKLLLEEDYFASASLEL
                     YQKLFISLLVLTAGQFCLTSLIALTNAAGPNNFLNLRWSDLILNALFIFLTYFVWT"
     gene            680..2566
                     /locus_tag="VCA20_000449"
     CDS             680..2566
                     /locus_tag="VCA20_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HEP1834311.1"
                     /translation="MTTIDISEESLARESSGLLRTLLKDRTTNKSIVWATHSYELLGK
                     GFGLYDRITPSKVTGSYANLIQPRSEKSKYEQKDRTKVRAEVFTPTWLVKKQIDAVDQ
                     DFQDLVLEAYLNLKWLEITCGEAPYMVSRYDTVTGQALDLEERVGFLDRKLHRLSQQV
                     HEEDAWFQGAVTAYQNAYGYEYQGDSLLLARENLLASFIDYYQAKFDQEPSSSQKQTI
                     AQIISYNVMQMDGLTYTSPYSQQAKEAVQLSLFEEIAEQTSQPQKAQIKDWKTNELID
                     FEHLSQGETTMKFDVVIGNPPYQDNTLGENDTYAPAIYHLFMEESYKIANKVALITPG
                     RYLFSPGRLPKIWHEKMLSDDHFKILFYSQDSSKVFPGTDIKGGVAIGYRDVHKNFGA
                     IELFIPFDQLRSIVKKVDSVSHGNISSLITNPLSHKFTEKLRETHPEVIELASKTFDL
                     RSNVLDKLDNVIFFDKKPNDNNEYMQILGLYKKQRAYKFIRRDFIKGPENIKNYKIFV
                     PESNGSGAIGEVLSTPLIGEPLIGHTQTFISIGDFNTKLEAEACLKYIKTKFSRALLG
                     VLKQTQHNPAPIWAKVPLQDFSSSSDIDWSQSVAEIDVQLYKKYGLSQEEIDFIESKV
                     REMD"
     gene            2566..3870
                     /locus_tag="VCA20_000450"
     CDS             2566..3870
                     /locus_tag="VCA20_000450"
                     /inference="COORDINATES: protein motif:HMM:NF025612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834312.1"
                     /translation="MTDNQQVATELFVVGNGLDLQCGLETTYENFLKYVFSQKKSELS
                     IDTEEIDLSIVADRFENRFDFYRTNWFEYSEKEVYSFVNIWYQIFLHEKMLENRNWSN
                     VESMIAHYLKEEHLIYNIASVIKYKKRKIVSLGKQTVYRAPSMQGSQTNFIAKLAYVL
                     VKKDLTLLQLSETESYQKIQEIYDNVVEYEVEDIQSIVADILLHELNELEYDFQCFLR
                     YRLSETPNYDEKINTLLRRMVDPEKDMFTAVPIPYHLLSFNYSSLWSQMFRPKFSPVK
                     FLDVHGKIPDNEGLRFSNIIFGVDHSLFQASELEYRFTKTYRTLKSYTQGFEKIGEGR
                     DIYTKDIQTIKFYGHSLAEADYAYFQQLFDYYDLYSNPNLSLIFFYSIYDKSKSHEIE
                     HEQIMAISRLIEKYGQTLDNKDHGKNLLTRLIQTKRLQMIKI"
     gene            3890..7150
                     /locus_tag="VCA20_000451"
     CDS             3890..7150
                     /locus_tag="VCA20_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEP1834313.1"
                     /translation="MSVLIKTSEEISPKIYAYTTPHVTTNDGWIKIGYTERQVEQRIK
                     EQTHTAGIEPSILWQQEARYIEEPDKGKPFKDHDFHQFLNFHAIERRPKTEWFYFNGH
                     PEQANHLFDQFVRHDYAGYQPGEQVDYQLRQEQEEAVQQTLTYIQTNQDARKKEFLWN
                     AKPRFGKTLATYDLVKRLNCDRVLIMTNRPAIANSWFDDFHKFIAGTTSYKFVSESDS
                     LKGRPALSRENFHKLLFTEEDAKEIAFLSLQDLKGSKYFGGAYDKLQWVAKTQWDLLV
                     IDEAHEGVDTEKTDVAFHQIQRNFTLHLSGTPFKALAKGKFSENQIYNWSYADEQAAK
                     QDWLSSSEQENPYENLPQLNMFTYQMSKMITDRVRDGADFGSETVDFAFDLNEFFATN
                     ESGSFIHEREVKEWLNTLTSNEKSPFSTRELRREIKHSFWLLDRVASAKALKDLLEDH
                     PTFDRYKIMLAAGSASQREAEDTEANGKSLDMVRKAIAENDYTITLSVGQLTTGVTIP
                     EWTAVLMLSNLQSPALYMQAAFRAQNPYSWVEGDKVYRKERAYVFDFAPERTLSLFSD
                     FANNLSLTTAGGAGTSQTREENIRNLLNFFPVIAEDREGKMVEIDAQAVMTIPSQIRA
                     AEVIKRGFMSNFLFDNIAGIFRASQLVLDILDELPVEQQGKISHAPATLEFPDVEVDE
                     EGQVQVAEDLVINRQEVLFGTKVYDLLDTVEELVDQVERVDKTFVKTVQKEISSTLVA
                     DLTAGYDLTNRESKQIEKQIHTEIESLIQRSEKEFDIQQAHLENEYQEALKETVDSQQ
                     QEELEQAYQVKKIEAHQAHQAELRQRLAEKVQDLPHDLVKQQEVKQRERTKAQAEDEI
                     RGHLRGFARTIPSFIMAYGDENLTLANFDSYVPDEVFIEVTGINLNQFRYLRDGGPEF
                     AGHLFDQPTFDQAIQEFLRKKAELANYFEDRTEDIFDYIPPQKTNQIFTPKKLVQKMV
                     DELEKENPGIFDDPSKTFIDLYMKSGIYITELVKRLYNSQGLQAAFPEGHERLKHILE
                     EQVYGFAPTAIIHKIALEFIFGTLPDIISRKNFLQVDTVPYAKEGRMQELIDKSFG"
     gene            7232..7771
                     /locus_tag="VCA20_000452"
     CDS             7232..7771
                     /locus_tag="VCA20_000452"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834314.1"
                     /translation="MANYVYQQVVCSKDFFETYFLDNYPIDEKKIDPPYISFNKLVGV
                     KNIGEYGELFGEFIYYGYGFTHKIRSDGLMVLKFATKRDVPIAAIRAAIQLDHSLEWY
                     AVEEYCIYVSRFYWNHGVQEEIYLLDDDDFGTWVEARYEFEEALADSVHIVWYYLLEN
                     KNNWLDRATLLDLPRYYEE"
     gene            7791..9494
                     /locus_tag="VCA20_000453"
     CDS             7791..9494
                     /locus_tag="VCA20_000453"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834315.1"
                     /translation="MWTKRIGYITEPGKLVDYFLSVGQFHDYLIGSFSYDSQSASLRL
                     TIEEDCLAESPSNEPALVWDLECQGVTDFRMQDMEGLSMWWIYEVLYDGTTFQFQLVN
                     GYFSFSAESFKLGIPHKPTTVEHPSITRLQYDYFLQEFEAGVNVDETAFRFETDNQDI
                     LRYIGYLGNEAPYWAGLSDLPDGRRFKTAKELFEAKIYDGQSLKERWSEVKIYSILSL
                     PLDDWLSCVPHSVETHVYEPDDEYGLYINGVELDGFIDEDGKPCPSCGKAPCYLDDYD
                     EHFCPYCNLWLTDEWWYDDELDYFEKRPLEPELLWKPSKMLRFCQVQFLNGGKHYSYY
                     CPDQTINKGDWVEVSVGKQAVKKDVPVVAIITAPADRPPYPLDKIKTVLGKSREWSEM
                     IAEKLQILLTHGHTCDLLEEKIPADSSIAYDLLKTPVGNFWLELNGQPIKMSVIAHYP
                     NLEDTYFVEGSYHLKPFEPDFTDFKSLSICTDIDFRIARLIDNLGGEHQYGNNWQVGD
                     NDIGIVAHPYSYDEEEVDENVLGIPYFREWIEDYQHLPCFSVAWKYYTSDEDLSIWFN
                     V"
     gene            complement(9613..11481)
                     /locus_tag="VCA20_000454"
     CDS             complement(9613..11481)
                     /locus_tag="VCA20_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEP1834316.1"
                     /translation="MTLVTNLKQHSHIITTALCLALILIGILLFQTEQGWAPILFISA
                     FVIGGYQSAKEGISELIFDKHLSVDLLMILAAIGSGLIGYWMEGALLIFIFSLSSTLE
                     ELAMEKSKNAIAALMNMTPPTARKIEENGDITVLDTAAIRIGDLLQVRKGDTVPLDAT
                     LISPQSIFDESMITGEPLPAEKMAGAAVIGGTINQGPTVTVQVTAEKGDALFDKIVQM
                     VENAQESKSKTATFIENMEDTYVKVVLVVVPLFILFAHFALGWDWLTAFYRGMILLTI
                     ASPCALVASSSPATLSAISRAARKGMIIKGGDIADNIANLEAIVFDKTGTLTIGKPEV
                     VGATYLGDEELIKQVVQAVEKQSSHPIAQALMTYTADSSAIALQSLEDVTGKGLVAGY
                     EGDSWKIGKAGFVVDSLVSPLSAGLLAQIDEAESTGKTLVYVSQNDVLVAIFMVEDSL
                     KPESKLLISQLKEMGVTPILLTGDQEKTARYVASQVGIDRVIANCLPTDKAAVIQELQ
                     TEFASVGMVGDGINDAPALAQANVSYAMGSGTDIAMESADIVLMEDLTRIPYSIRLSK
                     KMRGIIKQNIIFALSVIALLIISNLFQSINLPLGVVGHEGSTILVILNGLRLLYFK"
     gene            complement(11645..12100)
                     /locus_tag="VCA20_000455"
     CDS             complement(11645..12100)
                     /locus_tag="VCA20_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEP1834317.1"
                     /translation="MELHSHFNAENQTAFEHVIQHLKEKGVRITETRKAVVAYIIESD
                     DHPSAEMIYQDLLPNYPNMSLATVYNNLKLLLEEGFVTELKRTNDNTTYYDFMGHEHL
                     NVICEVCGKITDFMDVEIPSLKKEAHTQTGYKITKEVLSIYGICPDCQG"
     gene            12269..13018
                     /gene="truA"
                     /locus_tag="VCA20_000456"
     CDS             12269..13018
                     /gene="truA"
                     /locus_tag="VCA20_000456"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEP1834318.1"
                     /translation="MTRYKAIISYDGHDFSGFQCQPHARTVQEEIEKTLVRLNSGQPV
                     TIHGAGRTDAGVHAYGQVIHFDLAGSRDVEKLRFALDTQTPEDIDVVSVEQVADDFHC
                     RYAKHSKTYEFLVDIGRPKNPMMRHYATFYPYDLDLSLIEEAIQDLVGTHDFTGFTAS
                     GTSVEDKVRTITTATMEYDQRRQFLIFTFSGNGFLYKQVRNMVGTLLKIGNGRMPVGQ
                     IKRILAEKDRGLAGPTAAGNGLYLKEIIYED"
     gene            13008..13769
                     /locus_tag="VCA20_000457"
     CDS             13008..13769
                     /locus_tag="VCA20_000457"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004184219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEP1834319.1"
                     /translation="MKTKYILALSGNDIFSGGGLHADLTTYAVHKLHGFVAVTCLTAM
                     TEKGFEVIPTDAAVFAQQLASLKDVPFSAIKIGLLPTVDIAEQALAFIKAHPEIPVVL
                     DPVLVCKETHDTEVSQLRDELLKFLPYVSIITPNLAEAQLLLQKDIKTVEEMKQAAVA
                     LYDLGAKAVVIKGGNRLGDQQAVDVFYDGQEVVVLQSPLLSENNVGAGCTFASSIASQ
                     LLLGQEPLEAVRLSKDFVHAAIEKSDDYGVIQYEK"
     gene            13759..14244
                     /locus_tag="VCA20_000458"
     CDS             13759..14244
                     /locus_tag="VCA20_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEP1834320.1"
                     /translation="MRNKKTQELVLLAILTALSLVLAHFHLPTPSGFVTLLDVGVYFT
                     AFYLGKKEGAIVGGLAGLLIDFLLGYPQWAFFSLIFHGAQGYFAGWKGKSRYLGLALA
                     SISMVGGYYLASRLYFNDLKAIESVLSNTLQNTVGLVVGYILAQAIERIGAVNHVIKG
                     N"
     gene            14225..14791
                     /locus_tag="VCA20_000459"
     CDS             14225..14791
                     /locus_tag="VCA20_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEP1834321.1"
                     /translation="MSLKEIKEQTQQVVQEVLELSNLQKGQIFVLGLSSSEVIGGHIG
                     KNSSLEVGEVVVETILEILEPKGIYLAVQGCEHLNRALVVERELAIQKDLEIVNVLPT
                     LHAGGSGQLAAFKYMKDPVEVEFITAQAGVDIGDTAIGMHIKHVQVPLRPSLREIGQA
                     HVTALASRPKLIGGARAAYQEDKIRKGS"
     gene            14791..15405
                     /locus_tag="VCA20_000460"
     CDS             14791..15405
                     /locus_tag="VCA20_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1349 domain-containing protein"
                     /protein_id="HEP1834322.1"
                     /translation="MKEFTVKDLVWTRPPRQFTISDEEITITTHPQTDLWQKTYYHFV
                     NDNAPVLQMETEEDYFSFTVKTDFAGSHTRFDQCGIVLYQDSENWLKCSIEYENDDFQ
                     HLGSVVTNHGFSDWATTEISSQVKSMWFRLSRRGADYCFENSEDGIHFKQMRVCHFHQ
                     GTGKIAFGLYACSPENSSFTARFTNMELSDCKWQAHDGQEPDEI"
     gene            15678..16430
                     /locus_tag="VCA20_000461"
     CDS             15678..16430
                     /locus_tag="VCA20_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834323.1"
                     /translation="MTKGYQQRFSLSILLWMRQDKVRQAGMDYWSGGHAQIIAASPGL
                     LEYRQQHLSETEHSFWPKATGLETAIPEDRRIDGIAEVTYQKLLAPIGGRRQTALAFE
                     DEVNVFRRTLMHMGFPYSSRWYHTSTQVQTQLRDVLYFRRKDGVKSGSFKKFVQDGLA
                     SQLATISGVTEVRTQVYLPWNKATWNTPNVAHDNPKDASIILGFADQAAREAFYANLA
                     PQLNAEVVQYSSAVHAYHIEKTLPFVLDGKRM"
     gene            16710..17141
                     /locus_tag="VCA20_000462"
     CDS             16710..17141
                     /locus_tag="VCA20_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEP1834324.1"
                     /translation="MDTKFSVALHILTMISESKEILSSQALAESVGTNASYIRKVTAL
                     LKNAGLITSHQGRSGYQLSKSPKDISLLEIYLATQEVEHIRLFQIHQNANLTCPVGQH
                     IEGAMVPIFSSVEQELENQLSRQSLDNVITNLYQSAQVTRI"
     gene            17221..18090
                     /locus_tag="VCA20_000463"
                     /pseudo
     CDS             17221..18090
                     /locus_tag="VCA20_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003097216.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
     gene            18213..18347
                     /locus_tag="VCA20_000464"
                     /pseudo
     CDS             18213..18347
                     /locus_tag="VCA20_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646441.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
     gene            18374..19219
                     /locus_tag="VCA20_000465"
     CDS             18374..19219
                     /locus_tag="VCA20_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEP1834325.1"
                     /translation="MLYITTKNQYFTVSNNKIAYRELSKGKSDLPLVMLVHLAATLDN
                     WDPKLLDLLAEQHHVIVLDLPGVGASQGKVAPTIPGMAEQAVSIIKALGHEKINLLGL
                     SMGGFIAQEIVRLDSQLVNRLILAGTGPRGGFEVDKVTGKTFRYMLKAGLERVDPKRY
                     IFYNHDEAGRLEAEKVLGRMGQRSAAHVDKDMNVPGFLTQLKAIKRWGREPQDDMTFI
                     TQPTLIVNGDKDMQVPTENSYTMHEKIKNSQLIIYPNAGHGSIFQNAEDFSKALLAFL
                     EESNA"
     gene            19212..20077
                     /locus_tag="VCA20_000466"
                     /pseudo
     CDS             19212..20077
                     /locus_tag="VCA20_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637456.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
     gene            20070..21281
                     /locus_tag="VCA20_000467"
     CDS             20070..21281
                     /locus_tag="VCA20_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase family
                     protein"
                     /protein_id="HEP1834326.1"
                     /translation="MTKILLENLQLPNGQVLDNRFFKSAMSETMADSQGAPSDDLIAL
                     YDFWAKQGMGVLVTGNVMVDGCYLGEPGNVVLDSDAHLAQFRKWAAVGKKNGVPIWLQ
                     LNLPGKQMYRSINQTPIAPSAIPISGGSASAFRPPREMTVFDIKEARDKFIAAGLRAK
                     EAGFTGVQLHAAHGYLINQFLSLADNQRTDRYGGSLDNRMRFLVEVYQGLREKVRPDF
                     TIALKLNASDFKEESFGFEDCKHVVKTMSDLGIDLIEISGGNYETPVFGSEYENGAGF
                     VTYALALADLTSVPIVSTGGFRKVTQMEEAIKDGVSMIGLARPFVRRPSLVNDYRQDG
                     DLQLTTPRLTTGLPKLDKTLGLIIGVSYYEAQMKRLAKGKSVTVSQNAWLYLLQTVKA
                     HGLSALKPRRK"
     gene            complement(21376..23562)
                     /locus_tag="VCA20_000468"
     CDS             complement(21376..23562)
                     /locus_tag="VCA20_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003105430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEP1834327.1"
                     /translation="MIQIQFDSEKNYFYIQNPRMTYIIELYQNRHLLHRYWGPHISSF
                     KAWNQVPNQKKTFAAFRDFDNPTESLEYYPHESSLSYLGDYMEPSLEVTLANLSQIVH
                     FDFQSYQIIEGTPELEGLPHARNNQDSPAQTLILTFVDEKAQLTLEHYYTIFQNHGSI
                     VRSYKLLNHGTNPVMINKLMSASFDLPFDHQVLTSFSGSHQAEFQLQKKEIHPGRSMQ
                     STRRGASGPQYPPFLAISSANTDDFSGEVKAMSLIYSGEHAESVERNQYDFVRLQIGL
                     NPDSFSWKLEPNDSFQTPQAVLTYSKDGFNGMSQQFHRFTKDHLVPPQFAHRYAPILV
                     NSWEMTYFDVNHEKMSSLISKAAQLGFEAVVLDDGWFLGRENSRSSLGDWTVDRKKFP
                     DDLHPLIQICHEHGLQFGIWFEPEMISQRSLLAQEKPHWIVRSKNHVPLYSRHQYCLD
                     LSQTEVQDWLIETLSHFISTYKVDYIKWDMNRHLVEHESQITALGSAKEFAHRYMLGL
                     YRIIDSITKRFPDLLIENCSSGGGRLDYGMLYYFPQTWASDNTDGFDRQVIQDGTSYL
                     FHPYQITGHVSVTPNHQTQRMTPLQTRLDLASATNMGYELNLLEFSEEEEVVVMEHIK
                     EYKKQRPLIKHGNFYRLQSPFTSNQTAWLFESPDKSHYRLVAFRNIFKVSQHHTIIKI
                     PYLDQDTDYIDQFGREFSGAELVNSGIHIPFAASDFQSYRLELRRI"
     gene            complement(23543..24388)
                     /locus_tag="VCA20_000469"
     CDS             complement(23543..24388)
                     /locus_tag="VCA20_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEP1834328.1"
                     /translation="MEHYLKQYTDRFTDFSNKHISDLILYSSGIEQCIPNYSYSLKGR
                     EYHLITFVSQGKGSFEIEDKVYDVQANQLFIIPAGYWFKYTADATDPWRYSWIGFLGI
                     KSNFIYQAAERHQFVYDCRDASQYEGLIASILDSSGDTFSSFLKINGLMYSLLGDLTE
                     EIGILNQSTNQQLSTLARHFMDLHYHDPIQMTDVAEFVGVHPNYLANIFKKETNTTPK
                     SYLTTLRIRKAKELLLETSDSVSFISQSVGFSDSLSFSKFFKKETGLSPLHYRKEFHH
                     DTDSI"
     gene            24541..25869
                     /locus_tag="VCA20_000470"
     CDS             24541..25869
                     /locus_tag="VCA20_000470"
                     /inference="COORDINATES: protein motif:HMM:TIGR01322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEP1834329.1"
                     /translation="MENLYKNYFHLEAPRGLLNDPNGLTYFQGTYYVFHQWNRFDLHH
                     GYKEWGLFTSKDLIDWQHQGSALLPDSDKDVSGVYSGSALVIEDKLHIFYTGNSKING
                     KRRSLQCQAISADGKTFQKINSPITTPEVFTEHHRDPKIWTDSNGYHMIVGSQMKNGQ
                     GAIAYYQSIDGREWGYRGIFYTSEDLEQMAECPDYFELDGQSILLVCPQKRDLEKDTD
                     LSSYSAYYMGNLQKNEFCPQTDIQAVDDGFDFYAPQTFLDPKGRRLMWAWMSRMNSKE
                     EEACPTRKFGYLHCLTMPRELKVIDGKLYQLPLQEIPERKILIESLETVHYESEELAT
                     QSVIEMTWDESPLNLALALFDQQVTLEYKNQELILSRKSWSDGHVQSKKVPLKELNAL
                     QIFIDSSAMEFFINQGEKVMSLRYFLQQEKPSLDIRSSHHMKITNSKIES"
     gene            25877..27265
                     /locus_tag="VCA20_000471"
     CDS             25877..27265
                     /locus_tag="VCA20_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEP1834330.1"
                     /translation="MDNKTLAKEILKYVGGADNVKSAQHCATRLRIITKNQDLVDVKA
                     VENLDKVKGSFYNSGQYQIILGTGLVDKVYEEFMPLIGQAMSAKVDDELAEPEKLTFR
                     RAIRIFGDVFVPIIPVLVATGLFMGLRGLLTQEAVLGIFGLTPDDVPVQLIQFTQILT
                     DTAFAFLPALVAWSTFNIFGGTPILGMVLGLMLVNSILPNAYAVGSGEAEPMVFFGFI
                     KVVGYQASVLPAFMTALVTTKIEKWLKKTIPDSLDLILRPFLTLLSGLVLGLFIIGPV
                     FHEIEVYVLATVEFLLTLPFGLGGLIYGSFGQLLGILGIHHILSLLEINMLAKEGWNL
                     LNPVGTCGNVAQAGAVLAIAIKTVSNKMKQVAYPSALSASLGITEPAVFGVTLRLGKP
                     FIMSMIGGGIGGFFASIFQLKATGLGLTGIPGTLLYLNEQLPLYILTNLIAFGSTFAL
                     TWMFGYKKEDTL"
     gene            27544..28173
                     /locus_tag="VCA20_000472"
     CDS             27544..28173
                     /locus_tag="VCA20_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019335829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEP1834331.1"
                     /translation="MKIAVVAANGKAGSLIVKEAVARGFEVTAIVRGENKTVAQQVLQ
                     KDVFDLTADDVIGFDAVVDAVGDWTADTVHMVPDAAKHLAAILKDSQTRLLVVGGAAS
                     LYVNPERTLTVADVTEFPAEVLPVLNAHKEALEALRQVDDVNWTYVSPAGDFQADGER
                     TGRYILGGEELVLNSKGESVISYADYAIGMVDEIASGNHLKARISLVSE"
BASE COUNT         8299 a         5521 c         6485 g         7872 t
ORIGIN      
        1 aggtccagac aaaataagtc aggaagataa agagagcatt gaggatgaga tctgaccaac
       61 gcagattgag gaagttattt ggtccagcag catttgtcaa ggcgatcaag ctggttaggc
      121 aaaattgccc agcagtcaag acgagcaaac taatgaagag tttctgataa agttccagac
      181 tagctgaagc gaaataatct tcttccagaa gcaatttaca taacttttga agcaagtgag
      241 caatgtgact gagtaccgcc aagatgatta agattaatcc acttgttaaa aggatgagcc
      301 aaacactaat ctggtcaatg ggtttgttaa aggtgtattc ccaagagcct atcacaaatg
      361 agtcgcctct gaaaccagcc aaggtagaga aggtataaag agcaatcgtg aaatagaaga
      421 aatagcgaac acaaccagct aatagtccag ctgttttgag tagattggtt ttcatagcaa
      481 acctccgatt attattgttt tatgataata ttatccgtaa gattattatc ataaaacaat
      541 aatttttgaa atgcaagcgt ttttttaaaa aaagaaaaag acccaatgct ttttttattt
      601 tttacactcc tatgaaaacg attttattac ttgtaaagaa atggtataat agaaggtaaa
      661 taaagagaaa aggtgcctta tgacgaccat agatattagc gaagaatcat tagcaagaga
      721 gtcctctggt cttcttcgga ccctcttgaa agatcgaacg accaacaagt ccattgtgtg
      781 ggcgacacat tcttatgagt tattgggaaa agggtttggc ctgtatgatc gcatcacccc
      841 aagtaaagtg acgggttcct atgccaacct catccagcca cgctcagaaa agtccaagta
      901 cgagcaaaaa gaccgcacca aggttcgggc cgaggtattt accccgacct ggttggtcaa
      961 aaagcagatt gacgccgtgg accaagactt ccaagaccta gtcctagagg cctacctcaa
     1021 cctcaaatgg ttggaaatca cctgcgggga agccccctac atggtttccc gctacgatac
     1081 cgtgactgga caagcccttg accttgagga gcgggttggt tttttggacc gcaagctcca
     1141 tcgtctatct cagcaagtcc acgaggaaga tgcctggttc cagggggcag tgactgccta
     1201 ccagaacgcc tacggttatg agtatcaggg agattccctg ctcttggcgc gtgaaaacct
     1261 cctagccagc ttcatcgact actatcaagc caagtttgac caggaaccca gctccagcca
     1321 gaaacagacc attgcccaga ttatttccta caatgtcatg cagatggatg gtctgaccta
     1381 caccagcccc tactcccagc aagcaaagga agccgttcag ctcagccttt ttgaggaaat
     1441 agcagagcag accagccagc cacaaaaggc acaaatcaaa gattggaaaa caaatgagtt
     1501 gatagatttt gagcatctat cacaaggaga aaccactatg aaatttgatg ttgtcattgg
     1561 aaacccaccg tatcaggata atacacttgg tgaaaatgat acttatgctc cagcaatata
     1621 tcatttattt atggaagaat cctataaaat tgcgaacaaa gtagcgctaa ttacaccggg
     1681 aagatattta ttttctccgg gtagactgcc taaaatttgg catgagaaaa tgcttagtga
     1741 tgatcatttt aaaattttat tttattcaca agattctagt aaagtttttc ctggtacaga
     1801 tattaaaggt ggagttgcaa ttggatatag agatgttcat aaaaattttg gtgcaatcga
     1861 actatttatt ccgtttgatc aattgaggag tattgtaaag aaagtagaca gtgtcagtca
     1921 tggtaatatt tcgtcattga ttactaaccc gctcagtcat aagttcactg aaaagctcag
     1981 agaaactcat ccagaggtaa tagaacttgc tagtaagact tttgatttgc gttctaatgt
     2041 tcttgataaa ctagataatg taatcttttt tgataaaaag ccgaatgata ataatgaata
     2101 tatgcaaatt ttgggcctct acaagaaaca aagagcatat aaatttatta ggagagattt
     2161 tatcaaaggt cctgaaaata ttaaaaacta taaaatattt gttccagaat caaatggtag
     2221 cggagcaatt ggcgaagtgc tctctacacc cctaatcggg gaacccctaa tcgggcatac
     2281 tcagacgttt atatctattg gagattttaa tacgaaatta gaagcagagg cgtgtttaaa
     2341 atatattaag acgaaatttt cacgggcatt gttaggtgtg ttaaaacaga cgcaacacaa
     2401 ccctgctccg atttgggcaa aagtccctct ccaagatttt tcgtcaagct cggacatcga
     2461 ctggtcccag tcggtggcgg agattgacgt gcagttgtat aaaaaatatg gcctttctca
     2521 ggaagagatt gactttattg aatccaaagt tagggagatg gactgatgac agacaatcag
     2581 caagtagcca ctgaattatt tgtggttggg aatggtctgg atttacaatg tggtctcgaa
     2641 acgacatatg aaaacttttt aaaatatgtt ttttcgcaga aaaaaagtga gctatctatc
     2701 gatacagaag aaattgattt gagtattgta gctgataggt tcgagaatag atttgatttt
     2761 tatagaacta attggtttga gtatagtgaa aaagaagttt attcgtttgt aaatatttgg
     2821 tatcaaattt ttctacatga aaaaatgttg gaaaatagaa attggtcaaa cgttgaaagt
     2881 atgattgctc attatttgaa agaagagcat cttatttata atatcgcaag tgtcattaag
     2941 tacaagaaaa gaaaaatagt ttcccttgga aagcaaacgg tttacagagc tccaagtatg
     3001 caggggagtc agactaactt tattgcaaaa ttagcatatg tgctggttaa aaaagatttg
     3061 actttactac aactttcaga aacggaatct tatcagaaga ttcaagaaat ctatgataat
     3121 gttgttgaat atgaagtaga ggatattcag tctatagttg cagatatttt gctacatgaa
     3181 ttaaatgaat tggaatacga ctttcaatgc tttttacgat atagattgtc tgaaactcca
     3241 aattatgatg aaaaaataaa cactcttttg agaagaatgg ttgatccaga aaaggatatg
     3301 tttactgctg tcccaattcc ttatcattta cttagtttta attactcatc actttggtct
     3361 caaatgtttc ggccgaaatt ctctccagta aaatttttgg acgtacatgg taaaattccg
     3421 gataatgaag gtttgaggtt ttcaaatatt atttttggtg tggatcattc attgtttcaa
     3481 gctagtgaat tggaatatcg attcacaaag acatatcgta cattaaaatc ctatacacaa
     3541 ggttttgaaa aaataggtga gggtcgtgat atttatacaa aagatatcca aactattaag
     3601 ttctatggtc attctctagc agaagcggat tatgcatatt ttcagcagtt gtttgattat
     3661 tatgatttat actccaatcc aaatcttagc ttaattttct tttatagtat ttatgataaa
     3721 agtaaaagtc atgaaattga gcatgaacaa attatggcga tttcacggtt gattgagaaa
     3781 tacggtcaaa ctctagataa taaagatcac ggaaagaact tattaacacg actgatccaa
     3841 actaaacgat tacaaatgat taagatttaa tgcaaacaag aggtaatcta tgtccgtttt
     3901 gattaaaact tcagaagaga tttctccaaa aatctacgcc tataccacac cccatgtgac
     3961 gaccaatgac ggctggatca agattggcta taccgagcgt caggtggagc agaggattaa
     4021 ggagcagacc cacacggcgg ggattgagcc gtctattttg tggcagcaag aggctaggta
     4081 tatcgaagag cctgacaagg gcaagccctt caaagaccat gacttccacc agtttttgaa
     4141 cttccatgcc atcgaacgcc gtcccaagac cgagtggttt tatttcaatg gtcatccaga
     4201 acaggccaac cacctgtttg accagtttgt ccgccacgac tatgcaggct atcagccagg
     4261 ggagcaggtg gactaccagc tcagacagga gcaggaagag gctgtccagc aaaccttgac
     4321 ctatatccag accaatcagg acgcacgcaa gaaagagttt ctctggaatg ccaagccacg
     4381 ctttggcaag accctggcca cctatgactt ggtcaaacga ctcaactgtg accgggtctt
     4441 gattatgacc aaccggccag ccatcgccaa ttcttggttt gatgattttc ataagtttat
     4501 cgctggcact acctcctata agtttgtttc ggagtcggat tccctcaagg gcagaccagc
     4561 cctatcacga gagaacttcc ataaactcct ctttaccgag gaagacgcta aggaaatcgc
     4621 ctttttaagc cttcaagatc tgaaaggatc caaatacttc ggcggcgctt atgataaatt
     4681 gcagtgggta gccaagaccc agtgggatct cttggtcatt gacgaggccc atgaaggagt
     4741 ggatacggaa aaaacagatg ttgcctttca ccaaatccag cgcaacttta ccctccacct
     4801 gtctgggact ccctttaagg ctctagccaa gggcaagttt tcggaaaacc agatttacaa
     4861 ctggtcctat gcggatgagc aagctgccaa gcaagattgg ctctcaagct ccgagcaaga
     4921 aaatccctat gaaaaccttc cccagctcaa tatgttcacc taccagatgt ccaagatgat
     4981 cacggacagg gttcgagatg gggcggattt tggcagtgaa acggttgatt ttgcctttga
     5041 cttgaatgaa ttttttgcga ccaatgaatc aggcagcttt atccatgaga gagaagtcaa
     5101 agaatggctg aacaccctga cctctaatga aaaatcccca ttttcaacta gagagctccg
     5161 tcgggaaatc aagcacagct tttggctatt agaccgagtg gcctctgcca aggctttgaa
     5221 agacttgttg gaagaccatc cgacctttga ccggtataag attatgctgg ctgcaggttc
     5281 tgccagtcag cgagaagctg aggatacaga agccaatggc aagtcactcg atatggttag
     5341 gaaggccatt gctgagaatg actataccat caccctgtct gtcggtcagt tgacgactgg
     5401 tgtgaccatt ccagagtgga cagcagtctt gatgctgagc aatcttcaat ctccagccct
     5461 ctatatgcag gctgcctttc gtgcccaaaa cccttacagt tgggtggaag gagacaaggt
     5521 ttaccgcaaa gagcgggcct atgtttttga ttttgctcct gaacggaccc tgtcactctt
     5581 tagtgacttt gccaacaatc tcagtctaac gacagcgggt ggtgcaggaa ccagtcaaac
     5641 cagagaagaa aatatccgca atctgctcaa cttcttccct gttatcgcag aagataggga
     5701 aggcaagatg gtggaaattg acgctcaggc agttatgacc attccaagcc agatacgagc
     5761 agcagaagtc atcaagcgtg gctttatgtc caacttccta tttgacaata ttgctggtat
     5821 tttccgtgct agtcagctgg tcttggatat cttggatgaa ctccctgttg agcaacaagg
     5881 gaaaatcagt catgcaccgg ctacacttga atttccagat gttgaggtgg atgaagaagg
     5941 acaggttcag gtggcagaag acttggtcat caaccgtcaa gaagtccttt ttgggacaaa
     6001 agtttatgac ttgttggata cggtggaaga gttggttgac caggtagaaa gagttgataa
     6061 gacttttgtt aaaactgttc agaaagaaat atcttctacc ttggtggcag acctaactgc
     6121 tggctatgac ttgaccaatc gtgaaagcaa acaaattgag aaacagattc atacggagat
     6181 tgaaagcctc atccagcgta gcgaaaaaga atttgatatt cagcaggctc atctcgaaaa
     6241 tgaataccaa gaagctttga aggaaacggt ggatagtcag caacaagagg aactggagca
     6301 ggcctatcaa gtcaagaaaa tagaagcgca ccaagcccat caagcagagt tgcgacaacg
     6361 gctggcagaa aaagtgcagg atttgccgca tgatctggtc aaacagcagg aagtcaagca
     6421 acgagaacga actaaggctc aggctgagga cgagattcgt ggtcacttac gtggctttgc
     6481 acgcaccatt ccaagcttca tcatggctta tggggatgag aacttaactt tggcaaactt
     6541 cgatagctac gtaccagatg aagtcttcat agaggtaacg ggcatcaacc tcaaccagtt
     6601 ccgttatttg cgtgatggtg gacctgaatt tgccggccat ctctttgatc agccgacctt
     6661 tgatcaggcc atccaggaat ttttacggaa aaaagcagag ctggcaaatt attttgaaga
     6721 taggacagaa gacatctttg actatattcc acctcaaaag accaaccaaa tcttcacacc
     6781 gaagaagctt gttcagaaaa tggtggatga gttggaaaag gaaaatcctg gtatcttcga
     6841 tgacccatca aaaaccttta ttgacctcta tatgaagtca gggatctata tcacagagtt
     6901 agtcaaacgc ctctataata gccaaggttt acaagcagcc tttccagaag ggcatgagcg
     6961 gctcaagcat atcttggaag agcaagttta cggctttgcg ccaacggcta ttatccataa
     7021 gatcgctttg gagttcatct ttggcaccct acctgacatc atcagtcgga aaaacttctt
     7081 acaagtagat actgttcctt atgccaagga aggcaggatg caggagttga ttgataaatc
     7141 ttttggataa attgaaggaa aacttgtcat ttttggcaag ttttttagat aaggtgctat
     7201 actaaaacta ggaatgaagg gtggctatgt tatggcaaat tatgtttatc agcaggtcgt
     7261 ttgttccaaa gatttttttg aaacctactt tttagataac tacccgatag acgaaaaaaa
     7321 gattgatcca ccgtatatta gttttaacaa attggtgggt gttaaaaaca ttggtgagta
     7381 tggagaactt tttggtgagt ttatctatta tggatatggc ttcactcata agatacggtc
     7441 agatggcttg atggtcctaa agtttgcgac aaaacgtgat gttcctatcg ctgcgattag
     7501 agccgctatt caactggacc atagcttgga gtggtatgcg gttgaagaat attgtatcta
     7561 tgtttctcgt ttttattgga atcatggtgt ccaagaagag atttatttac tagatgatga
     7621 tgactttgga acatgggtag aggctagata tgagtttgaa gaagcactgg ccgactcagt
     7681 tcatattgtc tggtattatt tattggagaa taagaataac tggctcgaca gggctacctt
     7741 gcttgaccta ccgagatatt atgaggagtg atttgtgaga ggagaatatc atgtggacaa
     7801 aaagaatagg ctacattacc gaacctggga aattggttga ttatttctta tctgttggac
     7861 agtttcatga ttatttgatt ggttcttttt cctatgatag tcagtcagct agtctacgtt
     7921 taaccattga ggaggattgt ttagcggaaa gtccttccaa tgagccagcc ttggtttggg
     7981 atttggaatg ccagggagtg acagatttta gaatgcaaga catggaggga ctttcaatgt
     8041 ggtggattta tgaagtttta tatgatggca ctactttcca atttcagctt gtgaatggct
     8101 atttttcctt ctcagcagaa agttttaaat tgggaattcc ccataaaccg actacagtag
     8161 agcatccatc cattactcgt ttgcagtatg attatttctt gcaagaattt gaggcggggg
     8221 tgaatgttga tgagacggct tttcgttttg agacagataa tcaggatatt cttcgatata
     8281 ttggatattt aggaaatgaa gccccctact gggcaggttt gtcagatcta ccagatggta
     8341 gaagatttaa gacagccaag gaactttttg aggcaaaaat ctatgatggt caatccctca
     8401 aggaaagatg gagtgaggtc aagatttata gtattctttc tttgcctttg gatgactggt
     8461 tgagttgtgt accacattct gttgaaacac atgtgtatga accagatgat gagtatggtc
     8521 tctatatcaa tggggttgaa ttggatggtt ttattgatga ggatgggaag ccttgtccgt
     8581 cttgtggtaa agccccatgt tatttagatg attatgacga acatttttgt ccttattgca
     8641 atctctggtt gacggatgaa tggtggtatg atgacgagct tgattacttt gaaaaacgac
     8701 ctctggagcc agaactcttg tggaaaccga gtaaaatgtt gcggttttgc caggttcaat
     8761 ttttgaacgg tggcaagcat tattcctatt attgtcctga ccaaaccata aataagggag
     8821 attgggtgga agtttctgtt ggcaagcaag ctgtaaaaaa agatgttcca gtagtggcta
     8881 tcattaccgc tccagcagac cgtcctccct atcctttgga taaaataaaa accgtactag
     8941 gaaaatcaag agagtggtcg gaaatgattg cggaaaaact gcagatttta ctaactcacg
     9001 gccatacatg tgatctattg gaagaaaaaa tcccagccga ttcttccata gcttacgacc
     9061 tcttgaaaac tccagtcggc aatttctggt tggaattgaa tggtcagccc ataaaaatgt
     9121 cagttatagc ccattatcca aatcttgagg atacgtactt tgtggaagga agttatcatc
     9181 tgaaaccttt tgagccggat tttactgatt ttaagagttt atcgatttgt acagatattg
     9241 actttagaat tgcaagattg attgataact taggaggtga acatcaatac gggaacaatt
     9301 ggcaagttgg cgataatgat attggaattg tcgcgcaccc atactcatac gatgaagaag
     9361 aagtagatga aaatgtttta ggaattccct attttcgtga atggatagaa gactatcagc
     9421 atctaccttg cttttcggtt gcttggaaat actatacttc cgatgaggat ttatccatct
     9481 ggttcaatgt ctaactgaat ggagcaccct ctgcatgagc taggcttagg ttgtaaatct
     9541 ctattcattt caataataaa agtatttttc catatcaata aagaaaaccg ccaactacct
     9601 ggcggttttc agctatttaa aatacaagag tctcaaccca ttcaaaatca ccaaaatggt
     9661 agaaccttcg tgtccgacta cgccgagtgg caggttgata gactggaaga gattggaaat
     9721 gataagaagg gcaatgacag atagggcaaa gataatgttt tgcttgatga tgccccgcat
     9781 tttcttagag agacgaatgg agtaaggaat tctcgtcagg tcttccatga gtacgatgtc
     9841 agctgactcc atagcgatgt ccgttccgct tcccatagca tagctgacat tggcttgggc
     9901 aagggcagga gcgtcgttga taccgtcgcc caccatgccg acagacgcaa actcagtttg
     9961 cagttcttgg ataacagcag ccttatccgt cggcagacag ttggcaatca cacggtcaat
    10021 accgacctga ctcgccacgt aacgagccgt cttttcttgg tcgcccgtca agagaattgg
    10081 tgtcactccc atctctttca gctgggagat aaggagtttg ctctcaggtt tcaaactatc
    10141 ttccaccata aaaatcgcca caaggacatc attttgacta acatagacca gggttttccc
    10201 agtactttcc gcctcatcga tctgtgccag caagccagca gacaggggac tcaccaggct
    10261 gtccaccaca aatcccgcct taccgatttt ccagctgtcg ccctcatagc cagctactaa
    10321 tccttttccg gtcacgtctt ctaggctttg gagggcaata gcagagctgt cagccgtata
    10381 ggtcatgagg gcttgagcga tcgggtggct tgattgtttt tcaactgctt gaacaacctg
    10441 cttgataagc tcctcatcgc caagataggt cgcacctaca acttcaggtt taccaatggt
    10501 tagggtgccc gtcttgtcaa agacgattgc ctctagattg gcgatattat ccgcaatgtc
    10561 accacccttg ataatcatgc ccttgcgtgc tgcacggctg atggcagaaa gtgtcgccgg
    10621 tgaagaagat gctacaagtg cacatggaga agcgatagtc aagagaatca tgccacggta
    10681 gaaggcagtt aaccagtccc agcccagagc aaaatgagca aagagaatga agagtggtac
    10741 cactaccaaa acaaccttga cataggtatc ttccatattt tcaatgaaag tcgctgtttt
    10801 tgatttggat tcttgggcat tttcgaccat ctggacgatt ttatcgaaga gggcatcgcc
    10861 tttttcagcc gtaacttgta cggtcacagt tggaccttgg ttaatggttc cgccgataac
    10921 agcagcgcca gccattttct ccgcagggag cggctcgcct gtaatcatgg attcgtcaaa
    10981 gatggattgc ggactgatga gggtggcatc cagtgggacc gtgtcaccct tgcggacttg
    11041 caggaggtcg ccgatgcgga tggctgcggt gtctaagaca gtaatgtcac cattttcctc
    11101 gattttgcga gctgttggag gcgtcatgtt catcagagct gcaatagcat tcttgctttt
    11161 ttccatggct aattcttcca gtgtagaaga aagggagaag atgaagatga gtagagcccc
    11221 ttccatccag tagccaatta gaccagagcc gatagcagcc aaaatcatga gcaggtcgac
    11281 agacaggtgc ttgtcgaaaa tcagctcaga aatgccttcc ttggctgact ggtagccccc
    11341 gatgacaaag gctgaaatga agaggatagg agcccatccc tgttcagtct gaaacagaag
    11401 gatacctatt agaatgagcg ccaagcagag agcggtcgtg atgatgtggc tgtgttgttt
    11461 gaggttggtt acgagtgtca tagtttgctg ttccttttat ttagaatcat tatcatttaa
    11521 ctaagttaat tataacaatt ataaaaagaa agtcaagtta tttataataa ttctaaataa
    11581 tattgttctg ataattcaaa ggaaaataaa aaaagcgaca gaaagccgct ttgatattcg
    11641 tcttttagcc ctgacaatca ggacaaatgc cgtagatgga tagcacctct tttgtaattt
    11701 tgtaaccagt ttgggtatga gcttccttct tcaagctcgg gatttctaca tccatgaaat
    11761 cagtgatttt tccacagact tcacaaataa cattgaggtg ctcgtgcccc ataaagtcgt
    11821 aataagtagt attatcattg gtccgtttca attcagtcac gaacccttct tccaatagaa
    11881 gtttgaggtt gttgtaaaca gtcgccagac tcatgttggg gtagttaggg agtagatctt
    11941 gataaatcat ttcagcacta gggtggtcat cgctttcgat aatataggca acgactgctt
    12001 ttctagtttc ggtaatgcgt acaccctttt cttttagatg ttggatgacg tgttcaaaag
    12061 cagtttggtt ttctgcattg aagtgagaat ggagttccat agtttcctcc tttcgttatt
    12121 attttctata aaaattctaa gatacaatat tcagtttcat tatagtgtat ttgggaggtt
    12181 gtgtcaattt tgagacagga aatctgtcat aggacagttt ggtatctcat gacttgttta
    12241 catacttttc ctgtgctaca atcaaaacat gacaagatat aaggcaatta tttcctacga
    12301 cggacatgat ttttctggtt ttcagtgcca gccccatgct cgtacggttc aggaagaaat
    12361 cgaaaaaacg ttagtaagat tgaatagtgg tcagccagta accattcacg gtgcaggtcg
    12421 gacagatgcg ggggtccatg cttacggtca ggttatacac tttgacttgg ctggcagtag
    12481 agatgtggag aagctccgct ttgccctgga tacccaaacc cctgaggata ttgatgtggt
    12541 cagtgtggag caggtggcgg atgattttca ttgccgctat gccaagcaca gcaagaccta
    12601 tgagtttcta gtggatatcg ggcgacccaa gaatcctatg atgcgccact atgcgacctt
    12661 ctatccctat gatttggatt tgagcttgat agaagaagcc attcaggatc tggtggggac
    12721 ccatgacttc acaggcttta cggcttcagg gacctcggtc gaggacaagg tgcggaccat
    12781 cacgacggcg acaatggagt acgaccagcg gcggcagttc ttgattttta ccttttcagg
    12841 taacggtttt ttgtacaagc aggtgcggaa tatggtcggg accctcttga aaattggcaa
    12901 tggccgcatg cctgttgggc agattaagcg gattttagca gaaaaggatc gtggcttggc
    12961 gggaccgact gcggcgggta atggcctcta tctaaaggag attatttatg aagactaagt
    13021 atattttagc gctttcgggc aatgatattt tcagcggtgg cggtctccat gcggatctga
    13081 cgacctacgc tgtccacaag ctgcatggct ttgtggcggt gacctgcttg acagctatga
    13141 cggaaaaagg ttttgaggtc attccgacag atgcggcggt ctttgcccag cagctggcta
    13201 gtttgaagga tgtgcccttt tctgcgatta agattggctt gcttcccacg gtggacatag
    13261 cagagcaggc tttggcgttt atcaaggctc acccggagat tcctgtggtc ttggatccgg
    13321 tcttggtctg caaggaaacg cacgataccg aggtcagcca gttgcgagat gaactcttga
    13381 aattccttcc ctatgtcagc attatcacac caaatctggc agaagctcag ctattgctcc
    13441 aaaaggatat caaaacggtg gaagagatga agcaggcggc agtggccctg tatgacttgg
    13501 gagccaaggc ggtggtcatc aagggaggca atcgcttggg cgatcagcag gcagtggatg
    13561 ttttctacga cggtcaggaa gtggtggtgc tccagtcgcc tctgttgtca gaaaacaatg
    13621 taggggcagg ctgtaccttt gcgtccagca ttgccagtca gcttttgctg ggtcaggagc
    13681 ctttggaggc tgtcagactg tccaaggact ttgtccatgc agcgattgaa aaatcagatg
    13741 attatggggt gattcaatat gagaaataaa aaaacacaag aattagtatt attagctatc
    13801 ctaacagcct tgagcttggt cttggctcac ttccatttgc caacaccttc ggggtttgtg
    13861 acacttttgg atgttggggt atattttaca gccttttact tgggcaagaa agaaggagcc
    13921 attgtcggcg gtttggcagg gctcttgata gatttcctgc taggctatcc ccagtgggca
    13981 ttctttagtc taatattcca cggggcccaa ggttactttg caggatggaa agggaaaagt
    14041 cgttatcttg gacttgctct agcgagtatt agcatggttg gaggttatta tctagcttct
    14101 cgactttatt ttaatgacct caaagccatt gaaagtgttc taagcaatac cttgcaaaac
    14161 acagttggtc tggtagttgg ttacatcttg gctcaggcaa tagaaagaat tggtgcagta
    14221 aatcatgtca ttaaaggaaa ttaaggaaca gacccagcaa gttgtgcaag aagtcctaga
    14281 actcagtaat cttcaaaagg gacagatttt tgtccttgga ttgtcctcta gtgaagtcat
    14341 cggagggcac atcggcaaaa attccagtct ggaagttggt gaggtggttg ttgaaaccat
    14401 cttggaaatc ttggagccaa aaggtatcta tctagctgtc caaggctgtg aacacctcaa
    14461 tcgtgccttg gtggtggagc gggaattggc aattcagaag gacttggaaa tagtcaatgt
    14521 cttacccacc ctccatgcag gtggttcagg tcagttggct gccttcaagt atatgaagga
    14581 cccggtggaa gtcgagttta tcactgcaca ggctggtgtg gatatcggtg atacagctat
    14641 tggtatgcac atcaagcacg tccaagtgcc actcagacca agtttgcgtg agatcggtca
    14701 ggcccatgtc actgctcttg ccagccgtcc caaactaatc ggcggagctc gtgcagcata
    14761 tcaggaagat aagatacgga aaggttcata atgaaagaat ttacagtcaa ggatttagtg
    14821 tggacccgtc caccgagaca attcactatt tcagatgaag aaattaccat taccacccac
    14881 ccccagacgg atttgtggca gaagacctat taccattttg tgaatgacaa tgctcctgtc
    14941 ttgcaaatgg aaacggagga ggactatttt tcctttactg tgaagacgga ttttgcgggt
    15001 agtcatactc gatttgacca gtgtggaatt gtcctttatc aagattcgga gaattggctc
    15061 aaatgttcga tagaatatga aaatgatgac ttccaacact tgggaagtgt ggtgaccaat
    15121 cacggctttt ctgactgggc aactactgaa atcagttccc aagtcaaatc catgtggttt
    15181 aggctcagtc gcaggggagc tgattactgt tttgaaaatt ccgaagacgg tatccacttc
    15241 aaacaaatgc gggtttgcca ttttcatcaa gggacgggga aaatagcttt tgggctatat
    15301 gcctgttcgc cagaaaactc cagttttaca gctcgcttta ccaatatgga attgagcgac
    15361 tgcaaatggc aagcccatga tggccaagag ccagatgaaa tatagtgaga gttttaggta
    15421 aatagtaaca gaaaaatagt tgaaacgcat actcttcgaa aatcaaaatg atccgttgtc
    15481 aacttgcctt gaagaactcc tgttcaatct tcgccttcgt ttccaaggcg acttttgatt
    15541 tccattgagt gtcaggaagt aactttcgac tagtaagaga gttacttttt ttatcttttg
    15601 agccaaatcc ttgccaagaa gtcgggcttg tgataaagtt gttcctgtaa atgatacaag
    15661 aacaaaagag ggaaaatatg acaaaaggat accaacaacg attttcactg tcgattttat
    15721 tatggatgcg tcaggataaa gtacgtcaag cgggaatgga ctactggagt ggcgggcatg
    15781 cacagattat cgcagcttca ccgggtctat tagaataccg ccagcaacat ttgagtgaga
    15841 cagaacatag tttttggccg aaagcgacag gcttggaaac ggctattcct gaagaccgtc
    15901 ggattgatgg cattgcggaa gtaacctatc aaaagttatt ggcaccaata ggcggtcgcc
    15961 gtcagactgc tcttgccttt gaagatgagg tcaatgtttt ccgtcggacc ttgatgcaca
    16021 tgggcttccc ttattcatca cgttggtacc atactagtac tcaggtccaa acccaactgc
    16081 gtgatgttct ttacttccgt cgcaaagatg gtgtgaagag cggtagcttc aaaaaatttg
    16141 ttcaagatgg actagccagt caactggcga caatctctgg agtgacggaa gtacgtacac
    16201 aggtctatct tccttggaat aaagcgactt ggaatacccc aaatgttgcc catgacaatc
    16261 ctaaagatgc ctccatcatc ctaggttttg cggatcaggc ggcaagagag gccttctatg
    16321 ccaacctagc tccgcagttg aatgcagaag tggtgcaata ctcctcagca gtccacgctt
    16381 atcatataga aaaaaccttg ccatttgtcc tagacggcaa acgcatgtaa gacaagcgac
    16441 ttcttggtcg aatgctttgt taaaaaatat agtcgaatga attagctttt agacaaggaa
    16501 ctgtggtgca ggttgctagc acagtctagt ggctgtgcta ggttggagat ataacttgcg
    16561 aagcaagtca cattcgtaga gtacggcaag ccgaaagtga cgatgtatca aagttaattc
    16621 aaatgactat aaagcaaaag acttgccaag aagtctggct tgtgataaac ttgctcctgt
    16681 aaatgataca agaacggaaa ggaaaccaga tggatacaaa gttttcagta gcccttcata
    16741 tactgaccat gattagtgag agcaaggaaa tacttagctc gcaagcctta gctgagagcg
    16801 ttggcaccaa tgccagctac atccgcaagg tgactgcact tttgaaaaat gcaggcttaa
    16861 tcacttcgca ccaaggccgt tcaggctacc aattgagcaa gtcccctaag gatattagct
    16921 tattggaaat ttatcttgct acgcaagaag tggagcatat tcgcctattt cagatacacc
    16981 agaatgccaa tcttacttgt ccagttgggc agcatataga aggcgcaatg gttcccatct
    17041 tttccagcgt tgaacaagaa ctggaaaatc aactcagtcg tcaatcctta gataatgtga
    17101 ttactaatct ctatcaatcc gcacaagtaa cccgaatctg accttggtca gattttataa
    17161 actcaaacat agacctgtaa gtgatacagg aacaaaccaa ctaatagaaa agagaagaac
    17221 atgaaagcag cacaacacac agcttataat aaaaacaaca tccccctcaa cctaactgat
    17281 attgctaaac cacaaatcaa atcgaaccaa gtccttgtca aagtgaccgc agcaggcgtt
    17341 aacccattgg acaacatgat ttcacgcgga gatgtgaaat tgattgtccc ttacaaattg
    17401 ccacaaacag ctggaaatga agtagttggg atcatccaag aaatcggtgc aactgtaacc
    17461 aactttgcag caggtgatcg tgtcttcggt cgtcttccgc ttgatagtat cggtgctttt
    17521 gctgagtatg tagcggttga cgctcaggct cttgctaagg ttcctaccta cttgacagat
    17581 gaagaagcgg cagctgttcg tctgacagcc ttgaccatca tgcaggccct tgacctcatg
    17641 ggtgctgagg caggtaagac aatcttcatc tcaggcggaa ctggtggtgt cggcggtatg
    17701 gctattccga ttgccaaagc caagggcttg accgttatca caaatggtga tggggcaagt
    17761 gcagagcggg tgttgtcatt gggtgcagac cagtttatcg attacaagac agaagattac
    17821 accaaaaccc ttagcaatgt tgactacgtc ttggacacac ttggtggtgc agaaacagag
    17881 aaacaaatgt caatcatgaa aaaaggtgga cagctggttt cccttcgtgc catgccaaat
    17941 ggtgaatttg ccaaacgcat gaatctgcca aaatggaaac aaatcttgtt tggcctagct
    18001 ggtcgttcat ttgacaaaat ggcgaaaaaa tacggtgttc actaccattt cattttcgta
    18061 gagagcaatg gtcgccaact gcaagaagta tagtcgagtg aattagaagt aggacatata
    18121 taagtttgtc aatttgagaa ctctaaagtt attttaggag tgggttatta acactataac
    18181 ttaaaattgt ctttttccta attcaatccg ctatagcaga cattttcagc aagctcgaaa
    18241 tcaaaccgtc tatcgatacc gtgtatccat ttgaagaagt caacgcagca cttgataagg
    18301 ttgccaacgg tcgttcacgc ggaaaaaacg tcctcagttt caactaaaat aataaaatat
    18361 acaggggaaa atcatgttat atattacaac taaaaaccaa tacttcaccg tttcgaataa
    18421 taaaatcgcc taccgtgagc tcagcaaggg caagtctgac cttcctttgg tcatgctggt
    18481 tcacctagcc gcgaccttgg acaattggga tccaaaattg ctggatttgc tggctgaaca
    18541 gcaccatgtg attgtcctag accttcctgg cgtaggagct agtcaaggca aggtggctcc
    18601 aactattcca ggaatggctg agcaggctgt ttccattatc aaggctttgg gtcatgagaa
    18661 aatcaatttg ctgggtctgt ctatgggtgg tttcattgct caagaaatcg ttcgtctgga
    18721 tagtcaattg gtcaaccgtt tgattttagc aggtacaggt cctcgtggtg gttttgaagt
    18781 tgataaggta actggaaaaa ccttccgcta tatgctgaag gctggtttgg agcgtgtgga
    18841 tccaaaacgc tacatcttct acaatcatga tgaagcaggt cgcttggagg cagaaaaagt
    18901 cttgggtcgt atggggcaaa gaagtgctgc tcatgtagac aaggacatga atgttccagg
    18961 atttttgaca cagctgaaag ctatcaaacg ctggggaaga gagccgcaag atgacatgac
    19021 ctttatcacc caaccaacac ttatcgtcaa cggtgataag gacatgcagg ttccaacaga
    19081 aaattcttat accatgcatg agaaaatcaa aaatagccaa ctgatcatct atccaaatgc
    19141 aggacacggc tctatcttcc aaaatgcaga ggatttttcg aaagccttac tagccttttt
    19201 ggaggaaagc aatgcctaaa actattttaa tcacaggttc gacggatggg atcggcaaac
    19261 acctagccat gaaactagct agtgaaggtc atgaagtgat cttgcacggg cgaaatagcg
    19321 aaaagcttcg tgtagccctc agtgatattc aactaaaaac aggaaataca aagattcacg
    19381 gctaccttgc agatttttcc aagttagcag atgtctatcg tttcagccaa gaaattaaac
    19441 gcgattttga gcagattgat gtcttgttaa acaatgcggg agcttacttt ggtgatgctc
    19501 gcgtggcaac ggcagaaaat atcgagatga cctttatgct gtctgttcaa gttccttata
    19561 tcttgacgac ggagttactt cccttgctgg aaaaagcagc aggcagggtc attcacacct
    19621 cttccttcat gcaccatttt gcacaaacca gaggcttgga ttttggttta gagcagagat
    19681 attctgcagc catggcttac aataatgcca aactttacac catttggttg gcaatggctc
    19741 aggcagaggc cttagaaaag caaggttcat ctgtgaccgt caatacctac catcctggct
    19801 tgattgcgac aaatttgggc aatgatgggg tcaaacacaa cctgagaagt cggattctca
    19861 ccagtctgat gaaaccattt tccaaggatt tggaccaggg gattgaaacg ggttactact
    19921 tctgtcacca gaggtagctg gagtatcagg tcgttattat tcagagaaaa aagtggcacg
    19981 ggtcagcttg aaaggatttg atgtggcaaa gagccaagcc ttactagact atcttgataa
    20041 gaaaattcaa atattcaagg agagttacgg tgactaagat tttgttagaa aacctccaac
    20101 tgcccaatgg tcaggtctta gacaatcgct ttttcaaatc cgctatgagc gaaaccatgg
    20161 cagatagtca gggagctccc tcggatgact tgattgcctt atatgatttt tgggccaagc
    20221 agggaatggg tgtcttagtg acaggcaatg tcatggtgga tggttgctat cttggtgagc
    20281 caggcaatgt ggtccttgat tcagacgctc acttggctca atttcggaaa tgggcagccg
    20341 ttgggaaaaa gaacggtgtg cctatctggc tccagctcaa tctccctggc aaacagatgt
    20401 atcggtcgat caatcaaact ccgattgcac ccagtgccat tccgatttct ggaggctcag
    20461 cttcggcctt tcgtccaccg agagaaatga ccgtgtttga catcaaagaa gctagagata
    20521 aatttattgc agcaggcctt cgggcaaaag aagcaggatt tacaggtgta cagttacacg
    20581 ctgcacatgg ttacttgatc aatcaattcc tctcactagc agataatcag cggacggacc
    20641 gctatggtgg cagtctggac aatcgcatgc gattcttggt ggaagtctat caaggtctgc
    20701 gtgaaaaagt gagaccagat tttaccatcg ccttgaaatt gaatgcttct gattttaagg
    20761 aagagagctt tggttttgaa gattgtaagc atgtggtgaa aaccatgtct gacttgggta
    20821 tcgacctgat tgaaatttca ggtggcaatt atgagacacc tgtttttggc agtgaatatg
    20881 agaatggtgc tggatttgtg acctacgccc ttgctctagc agatttgact tctgttccta
    20941 ttgtttcaac aggcggtttc cgaaaagtca ctcagatgga agaggctatc aaggatggtg
    21001 tgtccatgat tggtcttgcc agaccctttg tccgtcgtcc aagccttgtc aatgattacc
    21061 gtcaagacgg tgatttgcaa ctgacaacgc ctcgcctgac aacaggtctt ccaaaactgg
    21121 ataagaccct tggtctcatt atcggtgtca gttattacga ggcccagatg aaacgcttgg
    21181 ccaagggaaa aagtgtcacc gtcagtcaaa atgcctggct ttatcttctc caaaccgtca
    21241 aggcacacgg tctgtcagcc ttgaagccga ggaggaagta gggaggctga gggctatcag
    21301 tagtagcaac agaatccaga aaaaatttga aatgaaaagt acactaggtt cacacatgtt
    21361 cctagtgtac tttttttata ttctgcgtaa ttccagccta taagactgaa aatcactggc
    21421 tgcaaatggg atatgaatac ccgaattgac taactcagct ccagaaaatt ctcttccaaa
    21481 ctgatctata tagtctgtat cttggtcaag ataaggaatc ttaataatgg tatgatgctg
    21541 agagaccttg aagatatttc taaaggcaac gagtcgatag tgggatttat caggactttc
    21601 aaacagccag gcagtctgat tactagtgaa aggagactgc aagcgataga aattaccgtg
    21661 ttttataagg ggacgctgtt ttttgtattc cttgatatgc tccataacca ccacttcctc
    21721 ttcctcagaa aactcaagaa gatttagttc gtagcccata tttgtagcag atgctaaatc
    21781 aaggcgagtt tgaagcgggg tcattcgttg agtttgatgg ttgggagtga cagaaacatg
    21841 cccagtgatt tgatagggat ggaagaggta gcttgtccca tcttgaataa cctgacggtc
    21901 aaatccgtcg gtattatcac tagcccaagt ctgtgggaaa tagtagagca tgccgtaatc
    21961 caatcgcccg ccaccactag aacagttttc aatcaacaag tcagggaagc gtttggtaat
    22021 agagtcaata attcggtaga gtcccagcat atagcgatgg gcaaactctt ttgcactacc
    22081 tagagcagtt atctgacttt catgttctac taaatgtcta ttcatgtccc atttgatata
    22141 gtcaaccttg taggttgaga taaagtgaga taaggtttca atcaaccagt cctgtacttc
    22201 tgtttggctc aaatctaaac aatattgatg acgactgtag agagggacgt gattttttga
    22261 gcggacaatc cagtgaggct tttcttgagc aagaaggctt ctttgggaga tcatttctgg
    22321 ttcaaaccag ataccaaatt gcaggccatg ttcatgacag atctgaatga gagggtggag
    22381 gtcatctgga aatttcttcc gatccactgt ccaatctcca agagaagaac ggctattctc
    22441 ccttcctaga aaccaaccgt catctaagac aacagcctca aaaccaagtt gagcagcttt
    22501 ggagatgagt gaagacatct tttcatggtt gacatcgaag taggtcatct cccagctgtt
    22561 gactaaaatt ggagcgtaac gatgtgcaaa ctgaggcgga acaagatggt ctttcgtaaa
    22621 gcgatgaaat tgttgcgaca tgccgttgaa gccgtcttta ctataggtga ggacagcttg
    22681 gggtgtctga aagctatcat ttggttcgag tttccatgaa aagctatctg gatttagacc
    22741 gatttgcaga cgaacaaagt catactgatt acgctctaca gattcagcgt gttcgccact
    22801 atagatcaag gacatggctt ttacttcacc tgagaagtca tctgtgtttg cagatgaaat
    22861 agcaagaaaa gggggatact gtggaccgct agcccctcta cgtgtagact gcatgcttcg
    22921 tcctgggtgg atttctttct tctgtaattg aaattcagcc tgatgggaac cactaaaact
    22981 tgtcagcact tgatgatcga agggaagatc aaaggaagca ctcatcaact tattgatcat
    23041 cacgggattt gttccgtggt tcagcagttt gtaagaacga acgatgctgc catgattttg
    23101 gaaaatagta taatagtgtt ccagtgtcag ttgggctttt tcatctacaa aagtcaatat
    23161 caaggtttgg gcaggactgt cctggttgtt tcgagcgtgt ggtaggcctt caagttcagg
    23221 agtcccttca ataatctgat aggattgaaa gtcaaaatgg acgatttgag aaagattagc
    23281 caaggtaact tcaagactag gctccatata gtcccctaga taagacaagg aagattcatg
    23341 aggataatat tcgagcgatt cagtcggatt gtcaaaatca cgaaaggcag caaaagtctt
    23401 tttctgattt gggacctgat tccatgcctt aaagctggaa atgtgtggcc cccagtaacg
    23461 atggagcaag tggcgattct ggtagagttc gataatataa gtcattctag gattctgaat
    23521 gtaaaaataa ttcttctctg agtcaaattg aatctgtatc atgatgaaat tcctttcgat
    23581 aatggagtgg tgaaagacct gtttcttttt tgaaaaactt ggagaaggaa agactatctg
    23641 aaaacccaac ggattgtgaa ataaaggata cggagtcgct ggtttcgaga agcagttctt
    23701 tggcctttcg aatccgtaaa gttgtaaggt agcttttggg agtggtattg gtttcctttt
    23761 taaaaatatt ggccagatag ttgggatgaa cccccacaaa ctctgcaaca tcggtcattt
    23821 ggataggatc atggtaatgt aaatccataa aatgtctagc aagtgtagaa agttgctgat
    23881 tggtagattg gtttaaaatc ccaatttcct cggtcaaatc acccagaaga ctatacatca
    23941 gtccgttaat tttcaagaag gaactaaagg tgtcacccga actgtcaaga atgcttgcaa
    24001 tcaggccttc atactggcta gcatctctac aatcatagac aaattggtgt ctttcggcag
    24061 cctgataaat aaagtttgac ttaattccca aaaaaccaat ccaagaatat ctccaagggt
    24121 cagtggcatc agctgtgtac ttgaaccaat atcctgcagg aataataaat agttgattgg
    24181 cctgtacatc atagactttg tcttcgattt caaaacttcc ttttccttga ctgacaaagg
    24241 tgataagatg atattcgcga ccttttagac tataggagta gttggggatg cattgttcga
    24301 taccgctact gtaaagaatt aagtccgaga tatgtttgtt actaaaatca gtaaaacggt
    24361 cggtatattg tttgagataa tgttccatat ctacagtata gcgaaacaag aaacaaaata
    24421 ccagacgaga ttagattttt ccataaagtg ctataaattt tctatatgat aatagcagtc
    24481 gattctctaa aatataaaca acaactagtt gaaagtgttt tatttttagg aggataatat
    24541 ttggaaaatc tatataaaaa ctattttcat cttgaagctc ctagagggtt attgaatgac
    24601 ccaaatggct taacctattt tcaaggaacc tattacgtgt tccaccaatg gaatcgattt
    24661 gatctccatc acggctataa ggaatgggga ttgtttacat caaaagattt aatagactgg
    24721 caacatcaag ggagtgcttt acttccagat tctgacaagg atgtgtcggg agtctattca
    24781 ggttcagctc ttgtgattga agataagctg catattttct atactggtaa tagtaaaata
    24841 aatgggaaaa ggcgatctct tcaatgtcag gcaatcagtg cagatggcaa aacctttcaa
    24901 aaaatcaact cccctatcac gactccagaa gtgtttacag aacaccaccg tgatccaaag
    24961 atatggactg actcaaatgg ttatcacatg atagttggta gtcaaatgaa aaacgggcaa
    25021 ggggcgatag cctactacca atcaattgat ggaagggagt ggggctaccg aggcatcttc
    25081 tatacttctg aggatttaga acagatggcg gagtgcccag attattttga acttgatgga
    25141 caatcaatcc ttctagtctg tccgcaaaaa cgtgacttgg agaaggatac agatctttca
    25201 agttactcag cctactacat gggaaatttg cagaaaaatg aattttgtcc ccaaacggac
    25261 attcaagctg tggacgatgg atttgatttc tacgctccac aaacattttt agatcccaaa
    25321 ggaagacgct tgatgtgggc atggatgagt cgtatgaact caaaagagga agaagcctgc
    25381 ccgacaagaa aatttggtta tttacattgt ctgacaatgc ctagagaatt aaaggtgatt
    25441 gatgggaaac tgtaccaatt acctctccaa gaaattcctg aaagaaaaat attgatagaa
    25501 tcgcttgaaa ccgttcatta tgaatcagag gagttagcta ctcagtctgt cattgaaatg
    25561 acctgggatg aatcaccttt gaaccttgca cttgccttat tcgaccaaca ggtgacactg
    25621 gaatacaaga atcaggaatt gatcttatca agaaaatcgt ggtcggatgg tcacgtacaa
    25681 tcaaaaaaag ttccgctaaa ggaactgaat gcacttcaaa tctttattga cagctctgcg
    25741 atggaattct ttataaacca aggtgagaaa gtcatgtcct tacgttactt tttacaacaa
    25801 gaaaagcctt ctcttgacat tcgttcctca catcacatga aaattaccaa ttcaaaaata
    25861 gaaagttgaa aagaaaatgg ataacaaaac actagcaaaa gaaatactaa agtatgtcgg
    25921 tggtgcagat aatgtcaagt ctgcccaaca ctgtgcaaca cggttaagaa tcattacaaa
    25981 aaatcaggat ctggttgatg taaaagctgt tgaaaattta gataaagtaa aaggtagctt
    26041 ttacaattcc ggccaatatc aaattatcct tgggacagga cttgttgata aggtttatga
    26101 agaatttatg cctttaattg gacaagcaat gtctgctaaa gttgatgatg agttagctga
    26161 accagaaaaa ctgacattta gacgagctat tcggattttt ggggatgtat ttgtacctat
    26221 tattcctgta ctggttgcca ctggtttgtt tatgggctta cgtgggctac tgactcagga
    26281 agctgttctt ggtatttttg gtttgacccc tgatgatgta cctgttcagt tgattcaatt
    26341 tactcaaatc ctaacagata cagcctttgc gtttttacca gcacttgtag cttggtctac
    26401 atttaatatc tttggtggaa cacctattct tggtatggtc ttaggcttga tgttggttaa
    26461 ctccattttg ccgaatgcct atgcggttgg ttcaggtgaa gctgaaccga tggtcttctt
    26521 tggttttatt aaggttgttg gttaccaggc ctctgtttta cctgccttta tgacggctct
    26581 tgttaccacg aagattgaaa aatggcttaa aaagacaatt ccagattctc ttgatttaat
    26641 cttacgtcca ttcttgacac tattgagtgg tttggtttta ggtttgttta ttatcggtcc
    26701 tgttttccat gaaattgagg tttatgtact tgctacggta gaatttttac taacattacc
    26761 atttggtcta ggtggattga tttatggctc atttgggcaa ttgcttggta ttttggggat
    26821 tcaccatatc ctaagtctcc ttgaaatcaa tatgcttgca aaagaagggt ggaacttact
    26881 aaatcctgtt ggaacctgtg ggaacgttgc tcaagcaggt gcagttctag ccattgccat
    26941 taagaccgta tcaaacaaaa tgaaacaggt agcctatcca tcggctttgt ctgcttcact
    27001 cggtattacg gaaccagctg tctttggtgt taccttgcga cttggaaaac catttatcat
    27061 gtcaatgatt ggtggtggta ttggagggtt cttcgcatct attttccaac tgaaagcgac
    27121 aggcttgggt ttgacaggga ttcctggaac tcttctatac ttgaatgaac aattaccgct
    27181 ttatatcctg acaaacctca ttgcatttgg ctctaccttt gcattgactt ggatgtttgg
    27241 atataagaaa gaagatactt tataaaaaga ataaaatagg agcagggcag aaatcgtcta
    27301 tcttagaaat gagcgatttc tgtcttgttc ccttttttca gctagtttcc aatgctgaat
    27361 tgtcgatgag aagtggctcg aggcttgaac ttggcttttt ttctacgata aataaaccta
    27421 cctacaattt ttgaaaaaag ttgtaataaa actgttgaca acttttagga gttaggatat
    27481 acgtagtgta cgtaaagttg taacaaaatg atttacaaca aaaacatttc ggaggaaaag
    27541 aaaatgaaaa tagcagtagt agcagcgaac gggaaagcag gtagcttgat tgtgaaagaa
    27601 gcggtagcac gtggttttga agtaacggcc atcgttcgtg gtgaaaataa gacagtcgca
    27661 cagcaggttc ttcaaaagga tgtttttgac ctgacagccg atgatgtgat tggttttgat
    27721 gcggtagtgg atgcagtagg tgactggaca gcagacactg tgcatatggt tcctgatgca
    27781 gccaaacatt tggcggctat tctaaaagat tcacaaacac gtttgcttgt ggttggtgga
    27841 gcagcaagcc tgtatgttaa tccagagcgt accttgactg tggcagatgt gacagagttt
    27901 ccagcggaag tattgcctgt gctaaatgct cacaaggaag cacttgaggc ccttcgtcaa
    27961 gtagatgatg ttaactggac ctatgtaagc ccggcaggtg atttccaagc agacggcgaa
    28021 cggacaggtc gctatattct tggcggtgaa gagttggtct tgaatagcaa gggagaaagc
    28081 gttatttcct atgcagatta tgccattggt atggttgacg agattgcatc aggtaatcat
    28141 ctcaaagcac gtattagcct tgtcagcgag taatact
//
LOCUS       DATUCG010000013        26842 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.013, whole genome shotgun sequence.
ACCESSION   DATUCG010000013 DATUCG010000000
VERSION     DATUCG010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26842
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.013"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(43..2583)
                     /gene="mutS"
                     /locus_tag="VCA20_000473"
     CDS             complement(43..2583)
                     /gene="mutS"
                     /locus_tag="VCA20_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEP1834332.1"
                     /translation="MATEKISPGMQQYLDIKAQYPDAFLLFRMGDFYELFYEDAVEAA
                     QILELSLTSRNKNAENPIPMAGVPYHAAQQYIDTLVELGHKVAIAEQMEDPKQAVGVV
                     KREVVQVITPGTVTDSSKMGADSNYLVAIDRQGVQFALSYMDVSTGQFFVTSLDDFTS
                     LCGEIRNLRARELVIGYALSEEEEQVFSNQMNLLLSFEDEVTEDVQLIDNSLTDLEKA
                     AAGKLLSYLHRTQMRDLSHLQKVVHYEIKDYLQMDYATKSSLDLLENGRTGKKHGSLY
                     WLLDETKTAMGMRLLRTWIDRPLIDLKRIENRQAVVQVFLDYFFERSDLVEALKGVYD
                     IERLASRVSFGKTMPKDLLQLSQTLGNIPAIKNILLQINEPALGNLVAGLDPIPELHA
                     LISSAIDPEAQGTITDGNIIRTGFDETLDQYRLVMREGAGWIAEIEAKEREASGINNL
                     KIDYNKKDGYYFHVTNSNLGNVPDHFFRKATLKNSERYGTEELAKIEGQMLEARDKSA
                     NLEYEIFMRIRQEVEKYIGRLQKLARTIATIDVLQAFAVVAEQQHLVCPRFTDQRELT
                     IDRGRHAVVEKVMGKQTYIPNSIHLNTDTHMQLITGPNMSGKSTYMRQLAVIVIMAQM
                     GSYVPADQAELPIFDAIFTRIGAADDLVSGQSTFMVEMMEANKAVRLATDRSLILFDE
                     LGRGTATYDGMALAQSIIEYIHDKIGAKTLFATHYHELTDLNQTLEHLENVHVSTLEK
                     DGQVTFLHKIAQGPADKSYGIHVAKIAGMPEELLQRADRILQTLENQAPTAPTHPIPS
                     VVEEPSGQIDLFADTPSHPVLDELEKLDVYNMTPMEVMMAVAELKKKL"
     gene            complement(2598..3038)
                     /gene="argR"
                     /locus_tag="VCA20_000474"
     CDS             complement(2598..3038)
                     /gene="argR"
                     /locus_tag="VCA20_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEP1834333.1"
                     /translation="MNKAGRHDLIKAMIRQEKIGRQTDIQQGLEARGVVVTQTTLSRD
                     LRELGVIKIHENGRSFYSLAIEEERVNFIQLLAQYAYKVDRASFILVLHSELGEAALM
                     ANIIDAEKPETILGTLAGANTLLVICRDDVAAQQVEAEINYYLN"
     gene            3145..4833
                     /locus_tag="VCA20_000475"
     CDS             3145..4833
                     /locus_tag="VCA20_000475"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEP1834334.1"
                     /translation="MNQKQVIAERLAAILPSLEVEAIYNLLEKPKSSEMGDIAFPAFS
                     LAKVERKAPQAIAADIVEKLDTTGFEKVVATGPYVNFFLDKAAISHQVLTDVITEKDQ
                     YGKLNIGQGRNVTIDMSSPNIAKPFSVGHLRSTVIGDALANIHEKLGYKPIRINHLGD
                     WGKQFGMLIVAYKLWGDKAAVEADPISELLKLYVRINAEAEEKPELDDEARQWFKKLE
                     DGDPEAHELWQWFRDESLVEFNRIYDKLDVTFDSYNGEAFYNDKMDEGIQILEEKGLL
                     QESKGARIVDLESYNLPPALIMKTDGATLYITRDMATAMYRKRTYDFVKSIYVVGQEQ
                     INHFKQLKAVLKEMDFDWSDDMTHITFGLVTKDKKKLSTRKGNIILLEPTLDEAISRA
                     LTQIEAKNPDLENKEEVAHAVGVGAVKFYDLKTDRDNGYDFDLEAMVSFEGETGPYVQ
                     YAYARIQSILRKANFVPSAENDYKLADAESWDIIKHIQNFSNVVERAGDKFDPSLIAK
                     YAINLAQAFNKYYAHTRILDESPERDSRLALAYATGLVLKEALRLLGVKAPEKM"
     gene            5013..6314
                     /locus_tag="VCA20_000476"
     CDS             5013..6314
                     /locus_tag="VCA20_000476"
                     /inference="COORDINATES: protein motif:HMM:NF020070.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEP1834335.1"
                     /translation="MKKFLRRKGIACQLLAAVFIVGINLQSPPSASANARGDDYPYTA
                     VDAVDPWRLYTRQCTSFVAFRLSKVNGFEIPPAYGNADVWGYRAQREGYRVDMSPAVG
                     SVAWWTSPMHVAWVSSIQGDMVEIEEYNYGTRYTYNRRLIHKNSVSGYIHFKDLTGSP
                     VSQTSPTPTQTHTSGLANSGTYTFTQQAPIKNEAKQSSTTLDYYYAGESVRYDRVLTA
                     DGYQWISYVSYSGQRRYIPIKQVQSSPAPQQPIVSTPTKPQGTIHITNINQAEGSFEV
                     IVTNVKSPKTIKSVSIPIWSDNNGQDDIIWYPAQRQSDGSYKVNVQASKHKNDRGLYH
                     IHVYYTDSSNQLEFITGTTTQLTAISQQTNQTNSNLPASGTYYFKSKAIVRNSPSQSA
                     SEITYYGAGSSVRYDRVITSEGRQWISYVSYSGVRRYIAIP"
     gene            complement(6364..6885)
                     /locus_tag="VCA20_000477"
     CDS             complement(6364..6885)
                     /locus_tag="VCA20_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEP1834336.1"
                     /translation="MKRPIHLYIFVILSSIASILRLFSAFVSTFNEERLRTSMQGVVG
                     IDVEELILVSRETANLQTGIIQKIVAVVMLGLLIAVIIFLFMKKNELASYLYIGYLFS
                     TLLLNTYSYLAGKGIANLYSDAALRDVTAAGMLGAYILNIVLFAIYFGVTIFFHLRKP
                     KEKPSTAINSTDI"
     gene            6997..7179
                     /locus_tag="VCA20_000478"
     CDS             6997..7179
                     /locus_tag="VCA20_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834337.1"
                     /translation="MKKLPTYIGVIIFVVTLLINWLLPSLAKVLNTFFIWTIVACLLY
                     TIYYWIRYWLYVRKQR"
     gene            complement(7219..8739)
                     /gene="malQ"
                     /locus_tag="VCA20_000479"
     CDS             complement(7219..8739)
                     /gene="malQ"
                     /locus_tag="VCA20_000479"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEP1834338.1"
                     /translation="MKVRQSGVLMHISSLPGKYGIGSMGQSAYEFVDFLVRTKQRYWQ
                     ILPLGTTSYGDSPYQSFSAFAGNTHFIDFDILIDKGWLAAEDLQGVDFGQNPTEVDYA
                     AIFYARRPLLEKVVANMKAQGLPEDYWMFLGENDFWIHTFAEYMAIKEHFDLKPWTEW
                     EDQGARLRYHDTLEYYRHLLADRIDYHRITQYLFFSQWKALKAYANAKGVEFVGDMPI
                     YIAADSADMWANSHFFKTDEEGKPSVVAGCPPDAFSEIGQLWGNPIYDWEAMKHDGFT
                     WWVARLRESFKIYDMVRIDHFIGFASFWEIPAGEETAVNGYRVEAPGFELFDTIKGEL
                     GDLNIIAEDLGSVTDKVIQLRDTTGFPGMKVLQFAFDPEGDSIEMPHNHPNNVVAYTG
                     THDNDTILGWYENETTKESRAFLDKYSNRREGETVSHALFRLLFGSPAFMAVATMQDL
                     LGLDGSARMNLPNTLGGNWTWRMTADQLTPEIEAELLDLTETYRRVNVHLVDEKSE"
     gene            9043..10302
                     /locus_tag="VCA20_000480"
     CDS             9043..10302
                     /locus_tag="VCA20_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEP1834339.1"
                     /translation="MKRNLLKSVALLAASTAVLAACSNSGSSTEASKSAEGSKELTVY
                     VDQGYESYINDVKAGFEKENGVSVTVKTGDALTGLDNLSLDNQSGSAPDVMMAPYDRV
                     GSLGSEGQLSELTLADDSKADDTTTALVTNGGKVYGSPAVIETLVLYYNKDLLTEAPK
                     TFAELETLAKDSKYAFTGEEGKTSAFLADWTNFYYTYGLLSGYGGYVFGENGTNPKDI
                     GLANEGAIKAIEYAKTWYEKWPQGLQDGTAANNLINTQFTDGKAAAIIEGPWKAASYK
                     EAGVNYGVATIPTLVNGKNYSAFGGGKAWVVPAGAKNQETAQKFVDFLTATDQQKALY
                     DATNEVPANTEAREYAVSKKDELTTAVINQFASAQPMPNISEMGSVWTPAGNMLFEAA
                     SGSKDAKTAATDAVKAIADEIAQKHSN"
     gene            10515..11810
                     /locus_tag="VCA20_000481"
     CDS             10515..11810
                     /locus_tag="VCA20_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003027817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEP1834340.1"
                     /translation="MTMKQNPGKALLLSLIPGLGQIYNKQKAKGYIFLGVTLAFLVYF
                     IAIAAGELGNLITLGSVRGQDNSLFMLIRGSFHLIITVVYLAFYALNLKDARDTAKRW
                     NSGMPVATTLKEMVKGIYENGFPYLLIIPSYIAMTFAIIFPVVVTLFIAFTNYDFKHL
                     PPSALLDWIGITNFTNIWKLSTFRTAFGSVLGWTIIWALCASTAQIVIGILTAIIANQ
                     PFIKGKRIFGVTFLLPWAVPAFVTILTFSNMFNDSVGAINTQVLPFLSKFLPFIYNFL
                     IPWKTDPFWTKVALIMMQAWLGFPYIYVLTLGILQSIPNDLYEAAYIDGASAIQKFRN
                     ITLPMILAVAAPTLISQYTFNFNNFSIIYLFNNGGPGSVGGGAGSTDILISWIYKLTT
                     GTSPQYSMAAAVTLIISMIVISISMIAFKKLNAFEMEDV"
     gene            11812..12654
                     /locus_tag="VCA20_000482"
     CDS             11812..12654
                     /locus_tag="VCA20_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEP1834341.1"
                     /translation="MKVSVKTRRILNQTLTYLYLIVLSVIIIYPLLITIMSAFKSGNV
                     VAFKLDSNINFTLDNFSKLFSETLYGTWYLNTLIIALLTMVIQTSIVVLAGYAYSRYN
                     FIARKQSLVFFLIIQMVPTMAALTAFFVMALMLNALNQSWFLIFLYVGGGIPMNAWLM
                     KGYFDTVPISLDESAKLDGAGHFRRFWQIVLPLVRPMIAVQALWAFMGPFGDYILSKF
                     LLREKEFYTVAVGLQTFISDVKNMKIAYFAAGAILIALPISILFFFLQKNFVSGLTSG
                     GDKG"
     gene            12714..13496
                     /locus_tag="VCA20_000483"
     CDS             12714..13496
                     /locus_tag="VCA20_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834342.1"
                     /translation="MLPYPFSYFSSIFSPRNMFANRRLMNLWQRLFTTLFLVALLLIP
                     SSLQTVNLETYPLENFVEGIYAPLTDQVVDDLRQHAVIENNHLTYTGNTNWEQVTFGD
                     TVSSSSDFTYQFANQSLTIRKGSDILSELTYEGLNTDDFASKEALTAAISKKWYQANR
                     AAVGLSITLISTFILTTNLLFILLGASFFLFLTKKSRFFHFETFAECYNFAINCLGLP
                     TLIACLIGLFGQPMTTLLTIQNILFVLCLLWVFFKTKFRDEI"
     gene            13506..14489
                     /locus_tag="VCA20_000484"
     CDS             13506..14489
                     /locus_tag="VCA20_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEP1834343.1"
                     /translation="MRATIKDVAKLAGVSPSTVTRVIQNSSAISQKTKDLVRKAMADL
                     NYHPNLNARSLVSSYTQVIGLVLPDDSDVFYQNPFFPTALRGISQVAADHNYAIQIST
                     GKNEEQRLEAISQMVYGKRVDGLIFLYSKPDDPLVQLAIQHKFPFLILGKADSPFISL
                     VDNDNIQAGFEATNYFINKGYKNIAFVAGNKELVVSQDRYTGYKNALKSHNIPLDENK
                     VKFVSGFLLEDSAYKISKKLLKQDLDAIVTTDTSVAEGVVKYLNEVGVKLPIISFDSV
                     KPKLDIEAYVDVHAIKLGRVAFNTLHQIINDNKEDKQVCYRRVIPHTITEL"
     gene            14513..16564
                     /gene="pulA"
                     /locus_tag="VCA20_000485"
     CDS             14513..16564
                     /gene="pulA"
                     /locus_tag="VCA20_000485"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015445376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HEP1834344.1"
                     /translation="MALRQFQASLDDVATIRLVMEKRFDSEHMSFSLESNDATSQLFI
                     HSCLEVDNLVIYYLTSLHALTLDKDYTIYDQDRNKTELGYGHIVRSTLFEQSYTYNRQ
                     DLGATYRPEATTFKLWAPISKQVFLILEGNPHAMTKGAKGVWEITVEGDLEAHSYHYL
                     HKVNGEWISVHDPYALSSAANSGDSYVINPDKLHKIRRAKTQRPISQAIIYEMSVRDF
                     SWQKEAGFKHRSQFLGLTESPTMDGMKLGMDYIRELGVTHIQLLPVYDFGSVDENKPQ
                     AVYNWGYDPMQYNLPEGSYSSQPNDPYARILELQEAIQAYHDADISVIMDVVYNHVYH
                     AEKYAFELIVPGYFYRYDDHGMRTDGTFCGNDVASERSMVRNYIKQSLRQWLELYGFD
                     GFRFDLMGILDSETINQIQTELSAIHPNVYLYGEGWKMATGLEFEKLAHQYNAEQLPE
                     ISFFNDDYRDTFKKLLLNPNRLVDKQLHEKVQHLLTGSRLSHFLTPQQSLNYIECHDN
                     ATAFDYFHIENPNWTPQQQKRAASFGLQLILISQGMAFIHSGQEFFRTKDEIDNTYNV
                     PDRINRLDWTRAIRYKEHIEFIQELITFRKNNPILSQDNYESIQETCDFYWLTEYVLR
                     YQVTSEEKTIQFIINFADSDFTYEREKDKAVLYNYPPVEERDQQEITIAGQSICILED
                     K"
     gene            complement(16718..17170)
                     /gene="nrdI"
                     /locus_tag="VCA20_000486"
     CDS             complement(16718..17170)
                     /gene="nrdI"
                     /locus_tag="VCA20_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEP1834345.1"
                     /translation="MKKKIYLVYISLSGNTESFVKRLKSFFQFQTDWEIELVHVKDLV
                     KQDIPFYQLDAPFVAFLPTYLEGGNGVDNGDVEILTNPLGDFIALGTNAELCLGVVGS
                     GNRNFNNQYCLTAKQYAERFDFPVIDTFELRGMQNDIERIGRKIMELM"
     gene            complement(17247..18179)
                     /locus_tag="VCA20_000487"
     CDS             complement(17247..18179)
                     /locus_tag="VCA20_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEP1834346.1"
                     /translation="MNKFFRKQRVRFLRKARKNKFWSVILGISREKYAERISGSIIYG
                     LLSSIAVNFFFRPGNVYSSGVTGLAQIVSALVASYAGWNVPIAVVYYGLNIPLLILAW
                     YKIGYKFTIFTFITVSFSSFFIQFMPPVTLTTDPLVNAIFGGLMLGTGIGFALRNNVS
                     SGGTDIVSIIIRKKTGRQVGSISLIVNIMIMLIAGMTFGWQYALYSMVALFISSRMTD
                     AIFVKQKRMQAMIITNHPERVIKKIHKKLNRGVTIINGAEGAYNHEQKTILITIITRA
                     EFNDFKHIMKKADPKAFVSVADNVNIIGRFVESD"
     gene            complement(18172..19923)
                     /gene="aspS"
                     /locus_tag="VCA20_000488"
     CDS             complement(18172..19923)
                     /gene="aspS"
                     /locus_tag="VCA20_000488"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEP1834347.1"
                     /translation="MKRSMYAGRVRKEHVGQEITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPESVEAEVMAKAESLRSEFVIEVTGTVVEREQANDNIPTGAVELQVTSLTV
                     LNTAKTTPFEIKDGIEASDDTRLRYRYLDLRRPEMLNNFKLRAAVTHSIRNYLDDLEF
                     IDVETPMLTKSTPEGARDYLVPSRVSKGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLNEVEIQDIVEGLIAKVLKDTKGIDVTLPFPRMAY
                     DHAMNFYGSDKPDTRFEMLLQDLTELVKEVDFKVFSEAPVVKAIVVKGAADSYSRKDI
                     DKLTDYAKQFGAKGLAWVKVDKGELAGPVAKFLTGITENLTASLQLEDKDLVLFVADE
                     LEVANNTLGALRSRLAKEQGLIDESKFNFLWIVDWPMFEWSEEEGRYMSAHHPFTLPT
                     EETDHHLDGDLAQVRAVAYDIVLNGYELGGGSLRINQKDMQERMFKALGFSAEDAHEQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGEDNIREVIAFPKNNKASDPMTQAPSTV
                     ASAQLEELALNITLENE"
     gene            complement(20018..20470)
                     /locus_tag="VCA20_000489"
     CDS             complement(20018..20470)
                     /locus_tag="VCA20_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEP1834348.1"
                     /translation="MKWEIKTFEQLSLQELYTILTLRTDVFVVEQACPYPEIDGKDPN
                     CLHLLGTDNGELVAYLRILPAGLSYDEVSIGRVVIKPSHRGKGLGRPMMEQAIAYITT
                     EWKESQIKIGAQAYLEKFYQSLGFEPISEVYLEDDIPHLDMLYRKPVN"
     gene            complement(20480..21391)
                     /locus_tag="VCA20_000490"
     CDS             complement(20480..21391)
                     /locus_tag="VCA20_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEP1834349.1"
                     /translation="MQRLGQTGLEASRIALGCMRMASLSGQETAQVLKTVVEQGINFF
                     DHADIYGGGESEIRFAQGAKLAGLKREDLLLQSKCGIRKGYFDFSKEYILESVDGILK
                     RLDTEYLDVLALHRPDALMEAEEVAEAFYLLKKVGKVHHFGVSNQNIHQMELLQSYLN
                     QPLAVNQLQLSPAHTPLIDAGLHVNMKDDAATMRDGGLIDYCQLKKITIQAWSPFLID
                     LQRGIFANHPDYAHLNQTIGEIAERYNVSHETIVVAWILRHPAKIQTIVGSMNPDRLT
                     KIAQASQITLTRPEWYEIYRSAGNILP"
     gene            complement(21461..22474)
                     /locus_tag="VCA20_000491"
     CDS             complement(21461..22474)
                     /locus_tag="VCA20_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEP1834350.1"
                     /translation="MTLLSIAIPSYNSEAYLHYCVHSLVMGGDKVEILIINDGSTDRT
                     QEIAEELANQFSNVRAIYQENKGHGGAVNTGIREAKGKYFKVVDSDDWVDTRAYLKIL
                     ESLQALEDENTPVDAFISNFVYEKEGQSRKKSMSYQSTLPENRIFGWEDVGAFSKGQY
                     MMMHSLIYRTDLLREVGLVLPEHTFYVDNIFVFTPLQAVKTMFYLPVDFYRYFIGRND
                     QSVNESVMIKCIDQQLKVNRILVDNLDLEAIDNPDLRSYLLNHVEITTIISCALLNRA
                     GTAEHMMKKQELWHYIRDNNPALFKIVRKGLLGQLTNLYGYPGRKISNAVYKIAKRIY
                     GFN"
     gene            complement(22637..24001)
                     /locus_tag="VCA20_000492"
     CDS             complement(22637..24001)
                     /locus_tag="VCA20_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEP1834351.1"
                     /translation="MKKKIVLLLGAIAVIVLIWSTIASQRQQSYVEAELRDQDKIWII
                     TDLHYLSQDLFDDGEAFSYIEKTAAGKDLRYGKERMEALVEQVEREQPSLLLVSGDLT
                     LNGEKQSMVELAQYFTRIEEKGTEVLVIPGNHDIASGWARAFKGDQQIVTDQVTAQQF
                     AELFAHHGYQQASSRDKASLSYLVKPFSNAWFLMIDSNIYSDGYGKGAPPTNGRIKKE
                     TLEWIEIQLRSAKEAGVSLIPVVHHNVLQQHAMLSKGYTLDNAADLKTLFNQYGIQFG
                     FSGHTHSQNIVKEDLGNVIYTEVVNGAFSIYPAIIGQLSLDDTSIHYQKTQLDMASWT
                     EKHQPSDPNLQDHVTYLQTVFDNTSDIMVHNVLNDERWYDGETADAISQFIMPANRAY
                     FSGEKLDQTWLDEKVFSSQAYQTLQAVSPRSFLADYLDVIIKRSQSRDVEKIVIKTKK
                     DFAQ"
     gene            complement(24087..25604)
                     /locus_tag="VCA20_000493"
     CDS             complement(24087..25604)
                     /locus_tag="VCA20_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HEP1834352.1"
                     /translation="MKKKAWLGLLMSLLAVLFLVACGGKSEKTATSSSSTTSSSSEEA
                     VSGASEKVYTDPTELKDSYDVIVVGSGGAGMSAAISAKDAGASVALLEKMPVIGGNTA
                     KSSAGMNASQTKFQEAEGIADSNDKFYEETLKGGKGTNDPELLRYLVDNSASAIDWLD
                     GMGITLSNLTTTGGMSEKRTHRPADGSAVGGYLVNGLYHNLVEREVPIFVNADVTKLQ
                     DKDGAVTGVEVKIAGETKKISAKAVVLATGGFGADLEMVAKLNPALKGYVTTNQEGST
                     GDGIVLAESEGAATVDMEQIQIHPSVEQETSYLITEAVRGEGAILVNSKGERFVNELE
                     TRDKVSAAINGLDPNYAYVIFDDALKDRVKAIAQYEEKGLVKTGATLADLAKEIGVPA
                     DALQTTLDTWNKAVADKNDAAFGRTSGMDHALNTGSYHAIKVAPGIHHTMGGVKINTN
                     TEVLKTDGSAISGLYAAGEVTGGVHGQNRIGGNAVADIIVFGRQAGEQAAGFAKK"
     gene            complement(25861..26772)
                     /locus_tag="VCA20_000494"
                     /pseudo
     CDS             complement(25861..26772)
                     /locus_tag="VCA20_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942077.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         7876 a         6364 c         4979 g         7623 t
ORIGIN      
        1 gtcacaaaaa aagccagaac aattgtcctg actttcattt tcttataatt ttttcttcaa
       61 ctccgccact gccatcatga cttccatagg ggtcatattg tagacgtcta gtttttccag
      121 ttcatcaagt actgggtgag aaggcgtgtc tgcgaagaga tcgatttgac ctgacggttc
      181 ttctactact gacggaattg ggtgagttgg tgcagtaggg gcttgatttt caagcgtctg
      241 caaaatcctg tccgcccgct gcaatagctc ctctggcatt cccgcaatct tagccacatg
      301 aatcccgtag gacttgtcag ctgggccttg ggcaatcttg tggaggaagg tgacttgacc
      361 gtccttctcc aaggtcgata catggacatt ctctaagtgt tccaaggtct ggttaaggtc
      421 tgtcaactca tggtagtggg tggcaaagag ggtcttggca ccaatcttgt cgtgaatgta
      481 ctcgatgata gactgggcca gagccatacc gtcataggtg gccgttcccc gtccaagttc
      541 gtcaaagagg atgagcgaac gatctgtcgc cagacggacc gctttattgg cttccatcat
      601 ttcgaccata aaggtcgatt gaccactaac caagtcgtct gccgccccaa ttcgggtgaa
      661 aatagcatcg aaaatcggta attcagcctg atccgcaggc acataggaac ccatctgcgc
      721 catgatgacg ataaccgcca actgccgcat ataggtagac ttaccactca tgtttggacc
      781 agtaatcagt tgcatatgcg tatcggtatt caagtgaata gagttgggaa tgtaggtctg
      841 cttgcccatg accttctcca ccaccgcatg acgaccacgg tcaatggtca gttctctttg
      901 atctgtgaag cgtggacaga ccaaatgttg ctgctccgcc acaactgcaa aggcctgcaa
      961 aacatcgatg gttgcaatgg tccgagccaa cttctgcaag cgaccgatat acttctcaac
     1021 ctcttggcga atccgcatga aaatctcgta ctccaaatta gcagacttat cacgcgcctc
     1081 caacatctgc ccttcgatct tagccaactc ttccgttcca tagcgctccg aatttttcaa
     1141 ggtcgcctta cggaaaaaat ggtccggcac attgcccaaa ttggaattgg tcacatggaa
     1201 atagtagccg tcttttttgt tgtaatcaat cttgagattg ttgataccag acgcttcacg
     1261 ctccttggcc tcaatctccg caatccaacc cgctccctca cgcatgacca gacgatactg
     1321 atccagcgtt tcatcaaagc ctgtacggat gatgtttcca tctgtgatgg tcccttgggc
     1381 ttctgggtca atagcagagc tgatgagagc gtgcagttct ggaattgggt ccaatccagc
     1441 taccagattg cctagagcag gctcattgat ttgtagcaag atattcttga ttgctgggat
     1501 atttcccaga gtctgggaga gctgcaagag atccttgggc atggtttttc caaaagacac
     1561 ccggctggcc aagcgttcga tgtcatagac accttttaaa gcctcgacca agtcactccg
     1621 ttcaaagaag taatccagaa aaacctgaac gacagcctga cgattctcaa tccgcttaag
     1681 atcaatcaag ggacgatcaa tccatgtccg caataggcgc atacccatgg ctgtcttggt
     1741 ttcgtctagc aaccagtaca aactaccatg cttcttgcct gtccgaccgt tttcaagcag
     1801 gtccagactg gacttggttg catagtccat ttgcagatag tccttgattt catagtggac
     1861 caccttttgc aagtggctca gatcccgcat ctgagtccga tgtaggtagc tgaggagttt
     1921 ccctgccgca gccttttcca agtctgtcag cgaattgtcg attagctgga catcttctgt
     1981 cacctcatcc tcaaaagaga gcaggaggtt catctggttc gaaaagacct gctcttcttc
     2041 ctcagataaa gcatagccga tgaccaattc acgtgcccgt aaattgcgga tctcaccgca
     2101 aagactagta aaatcatcca aactagtcac aaaaaactga cctgttgaca catccatata
     2161 agaaagtgcg aactgcactc cctgacggtc aatcgcaacc aaatagttac tgtctgctcc
     2221 cattttggac gagtctgtta ccgtaccagg agtaatgacc tgcactactt cccgcttgac
     2281 cacgccgact gcctgcttag gatcttccat ctgctcggca attgcgacct tgtgccccaa
     2341 ttcaacaagt gtgtcgatat actgttgtgc cgcgtgatac ggtacccccg ccataggaat
     2401 cggattctcc gcattcttat tccgactggt caaggacaat tccaaaatct gtgccgcttc
     2461 taccgcatct tcataaaata attcataaaa gtctcccata cggaaaagca aaaaagcatc
     2521 tggatactgt gcttttatat ccagatactg ctgcattccc ggagaaattt tttctgttgc
     2581 catcatctct ccctgttcta attcaaatag tagttaatct ccgcttcaac ttgctgagcc
     2641 gctacatcat ctcgacaaat aactaaaagt gtattagctc ccgccagcgt tcccaaaata
     2701 gtctctggct tttctgcatc aatgatattg gccatcaagg ccgcttctcc cagttctgaa
     2761 tgaaggacca gaataaaact agccctatct actttatacg catattgtgc caataattgg
     2821 ataaaattca cgcgctcttc ttcgatagct aaggaataaa atgaacgccc attctcatga
     2881 atttttatca ctcccaactc acgtaaatca cgagacaagg ttgtctgggt taccactacc
     2941 cctcgagcct ctaaaccttg ctgaatatcc gtttgtcgcc ctattttttc ttgacgaatc
     3001 atggccttta ttaaatcatg tcgtcctgct ttattcatca ttttacctca aaatgtatat
     3061 ttatagaaat attataacac ttttccccgc tattagccta gaaatgtgtt aaaatagaat
     3121 caataacttt ggaggaaatc ctatatgaat caaaaacaag tgattgcaga aagattggct
     3181 gccatccttc cgagtttgga agtggaagct atctacaatc tgctagaaaa accaaaatca
     3241 tcagaaatgg gcgatattgc cttccctgcc ttctcacttg ctaaagtgga acgcaaggct
     3301 ccacaggcta tcgcagcgga catcgttgaa aaactcgata caactggctt tgagaaggtt
     3361 gtagctaccg gtccttacgt caacttcttc ttggacaagg ctgcgatttc ccaccaagtc
     3421 ttgacagatg ttattaccga aaaagaccaa tacggtaaac tcaacattgg tcaaggacgc
     3481 aatgtaacca tcgacatgtc cagcccaaac attgctaaac cattctcagt tggacatttg
     3541 cgctcaaccg ttatcggtga tgcccttgct aacatccacg aaaagcttgg ctacaagcca
     3601 atccgcatca accacttggg tgactggggt aaacaatttg gtatgttgat tgttgcctac
     3661 aaactttggg gtgacaaggc tgcggtcgaa gcagacccaa tttcagaact cctcaaactc
     3721 tatgtgcgta tcaacgctga agcggaagaa aagcctgagc tggacgacga agcacgtcaa
     3781 tggttcaaaa aattggaaga tggcgatcca gaagcacacg agctatggca atggttccgt
     3841 gatgaaagct tggtcgaatt caaccgcatt tacgacaaac tcgatgtaac attcgacagc
     3901 tataatggcg aagccttcta caacgataag atggacgaag gcatccaaat cttagaagaa
     3961 aaaggacttc ttcaagaatc taaaggtgcg agaatcgttg accttgaaag ctacaatctt
     4021 ccaccagccc tcatcatgaa aacagacggt gcaacacttt acatcacacg tgacatggca
     4081 acagctatgt accgcaaacg cacttacgac tttgtgaaaa gcatctatgt cgttggtcag
     4141 gagcaaatca accacttcaa acaactcaag gctgtcttga aagaaatgga cttcgactgg
     4201 agcgacgata tgacccatat caccttcggt ctggttacca aggacaagaa aaagctatcc
     4261 actcgtaaag gaaatatcat cctactcgag ccaactctag atgaagctat ttcacgcgcc
     4321 cttactcaaa tcgaagctaa aaaccctgac cttgaaaaca aggaagaagt agcacacgca
     4381 gttggtgtag gcgctgttaa gttctacgac ctcaaaaccg accgagacaa cggctacgac
     4441 ttcgacctag aagctatggt ttccttcgag ggtgaaacag gtccttatgt acaatacgca
     4501 tacgcccgca tccagtctat cctacgcaag gcaaactttg taccaagcgc agaaaatgac
     4561 tacaaactgg ctgatgcaga aagctgggac atcatcaagc acatccaaaa cttctcaaac
     4621 gttgtagaac gtgctggtga caaatttgac ccatccctca ttgctaaata tgctatcaac
     4681 ctggcgcaag ccttcaacaa gtactacgca cacactcgta tcttggatga aagcccagag
     4741 cgtgacagcc gtctagcact tgcctacgca acaggacttg tcctcaaaga agctctacgt
     4801 cttctcggcg taaaagcacc agaaaaaatg taatcaaagc cttcggccat cagccgaggc
     4861 tttttttcta ccacattgcc tactctcgtt tccgattcct tatcacaaca actccttatc
     4921 ccaaatgaaa atcaaaaaga ttcatttcaa ttacgaatat atagatggga ggtacacgtg
     4981 atacctctac atctactaca caaggagaaa taatgaaaaa atttttgaga agaaaaggaa
     5041 tcgcctgtca gcttctagcc gccgttttta ttgtcggtat caatttacaa tccccaccgt
     5101 cagcctcagc caatgcacga ggagatgact atccctacac agcagttgat gcagtcgatc
     5161 catggagact ctacacacgc cagtgcacct cctttgtagc ctttcgactc agtaaagtca
     5221 acggttttga aatcccacca gcttacggta atgcggatgt ctggggatac cgagcgcagc
     5281 gtgaagggta tcgcgtggat atgagccctg ccgtgggatc agtagcttgg tggacctccc
     5341 ctatgcacgt tgcctgggta tctagcatcc aaggtgacat ggtcgaaatc gaagaataca
     5401 actacggtac tcgatacacc tacaatcgcc gtctgatcca taagaactct gtcagtggct
     5461 atattcactt caaggaccta actggctctc ctgtcagtca gaccagtcct acacctactc
     5521 agacgcacac atctggctta gcaaacagtg gtacctatac ctttacccag caagcaccaa
     5581 ttaaaaatga agccaaacaa tctagcacaa ccttggatta ctattatgct ggtgagagcg
     5641 tacgctacga ccgagttcta acagctgacg gctaccagtg gattagctat gtcagctata
     5701 gtggtcaacg acgctacatt cctatcaaac aggtacagtc ctcccctgca ccacagcagc
     5761 caattgtatc aactcctaca aaacctcaag gaacaatcca cattacaaat atcaatcaag
     5821 cagagggtag ttttgaagtt atcgtcacca atgtaaaatc tcccaagact atcaaatcag
     5881 tatctattcc aatttggtca gataataatg ggcaagatga tatcatctgg tatcctgctc
     5941 agcgacaaag cgatggcagc tacaaggtaa acgttcaagc aagtaaacat aaaaatgacc
     6001 gagggctcta ccacatccat gtctattaca cagattcctc aaaccaactc gaattcatta
     6061 ctggaactac aactcaacta acagcaatat ctcaacaaac caatcaaaca aacagtaatt
     6121 taccagcatc gggtacctat tatttcaaga gcaaagccat tgtccgcaat agccctagtc
     6181 agtccgccag tgaaatcacc tactacggtg ctggttcttc tgtccgctat gaccgagtta
     6241 tcacatccga aggacgccag tggatcagct atgtcagcta ttcaggagtc cgtcgctaca
     6301 tcgccatccc ataaaacaaa agaaactgca ggttcactat gcaccatgca gttttttaga
     6361 tgattaaata tcagtagaat tgatagctgt gctcggtttt tctttcggtt tacgcagatg
     6421 gaagaaaatg gtcacaccaa agtaaatggc aaataggaca atgttaagga tgtaagcccc
     6481 tagcatacct gctgctgtca catcacgcaa ggctgcatct gagtagaggt tggcaatccc
     6541 cttacctgct aggtagctat aggtattcag cagtaaggtc gaaaatagat agcctatgta
     6601 gagataggaa gccagctcgt ttttcttcat gaataggaaa ataatgactg caattagtag
     6661 accaagcatg accacggcta ctatcttttg aatgatacct gtctggagat tagctgtttc
     6721 tcgcgaaaca agaatcaatt cttctacatc aatcccaaca actccctgca tagacgtacg
     6781 aagtcgctcc tcattaaaag tcgacacaaa ggcactaaac agccttaaaa tactggcaat
     6841 actggatagt atcacaaaaa tatacaaatg aattggtctt ttcataatag cctccttttt
     6901 tctatatttt atactcattt ttttatattg tcaatcatat atttcatttg actgcaaata
     6961 taattttgtt atactaaatt caaaggagga aaactcatga agaaattacc cacctatatc
     7021 ggagtgatta tttttgtagt tacgctactg attaactggt tattgccaag tcttgctaaa
     7081 gtgctgaata catttttcat ttggactatc gtagcctgcc tactctacac catctactac
     7141 tggattcgtt actggctcta tgtccgtaaa cagagataac aaagactcct ctaagtcagt
     7201 tgcttagagg agttcatttt attctgattt ttcatcaacc aaatgcacat taacacgacg
     7261 ataggtttct gtaaggtcta gcaattccgc ttcaatctct ggtgtcaatt gatccgccgt
     7321 cattctccaa gtccagttgc caccaagagt gtttggcaag ttcatgcgag ctgaaccatc
     7381 caaaccaagt aggtcctgca tagtagccac cgccataaag gcaggtgaac caaagagcag
     7441 acggaaaaga gcatgactga ccgtttcccc ctcacgacga ttgctgtact tatctaagaa
     7501 tgcacgcgat tctttcgttg tttcattttc gtaccagcca agaatcgtat cattgtcatg
     7561 ggttcccgta tatgccacca cattattagg atggttatgt ggcatttcaa tgctgtcgcc
     7621 ctcagggtca aaggcaaatt gcaggacttt cataccagga aaacctgttg tatcacgtaa
     7681 ttggatgacc ttgtctgtca cagagcccaa atcctcagct atgatattta agtctccgag
     7741 ttcacccttg attgtatcaa ataattcaaa acctggtgct tcgacacgat agccatttac
     7801 agctgtttct tcacccgctg gaatttccca gaaggaggca aagccgataa agtggtcgat
     7861 acgtaccata tcgtatatct taaaggattc acgtaaacga gcaacccacc aagtaaatcc
     7921 gtcatgcttc atcgcttccc aatcataaat cggattgccc caaagctgac caatttccga
     7981 aaaggcatct ggtgggcatc ctgcaaccac actcggctta ccttcttcat ccgttttaaa
     8041 gaaatgtgaa ttagcccaca tgtcggcaga atcagccgcg atataaatcg gcatatcccc
     8101 aacaaactca acacctttgg cattggcata agcctttaaa gccttccatt gtgaaaagaa
     8161 taaatattgt gtaatgcggt gataatcaat tcgatctgct aacaagtgac gataatattc
     8221 caaggtatcg tggtaacgta agcgagcccc ttgatcttcc cactctgtcc aaggtttgag
     8281 atcgaaatgc tcttttatag ccatatactc cgcaaaggta tgaatccaaa agtcattctc
     8341 ccctaagaac atccaataat cttctggcaa gccttgtgct ttcatgttgg caactacctt
     8401 ctccagtaat ggacgtcgag cataaaaaat ggctgcgtaa tctacctctg taggattctg
     8461 cccaaagtca acaccttgta aatcctctgc tgccagccag cctttatcaa tcaatatatc
     8521 gaaatcaata aaatgagtat tccctgcaaa agctgaaaat gactggtaag gagaatctcc
     8581 ataactggtt gttcccaaag gtaagatttg ccaatagcgt tgcttagttc ttaccaaaaa
     8641 atcaacaaat tcataggccg attgtcccat ggaaccaatt ccatatttcc caggtaatga
     8701 tgaaatgtgc atcagcacac cactctgtct tactttcata agttctccta tctaatcagc
     8761 ttgcatacgc aaacgttttc tcttttttat tatatactga aaacggtaac aaatcaagga
     8821 atttccattt aattctgtta gttcacatgc cattccatat ttcttttcat taatatgtgc
     8881 aaaaaaaaat tcttatattt ttttacactc aaaaaaattt ttaaaaattt ttataaaaac
     8941 gcttgcaaac ggattcaatc tagtgtataa tgaagtcaac ttaggaaaac gtttgcgcaa
     9001 agcaagcgtc acacaaaact atttatcttc ggagggaaga aaatgaaacg caatctcctt
     9061 aagagcgttg ctcttcttgc tgcatcaact gctgttttag ctgcatgctc aaactcaggt
     9121 tcatcaacag aggcatctaa gtctgctgaa ggtagcaaag aattgactgt ctacgttgac
     9181 caaggttatg aatcttacat caacgatgtc aaagctggct tcgaaaaaga aaatggtgta
     9241 tctgttaccg ttaaaactgg tgatgcttta actggtttgg ataacttatc attggataac
     9301 caatctggtt cagccccaga cgttatgatg gcaccatacg accgtgtagg tagccttggt
     9361 tctgaaggtc aactatctga attgactctt gcagatgata gcaaagcaga tgacacaaca
     9421 actgctcttg taacaaacgg tggtaaagtt tacggttcac ctgcggttat cgaaacactt
     9481 gttctttact acaacaaaga cttgttgact gaagctccta agacatttgc agaacttgaa
     9541 acacttgcta aagacagcaa atacgcattt actggtgaag agggtaaaac ttcagcattc
     9601 ctagctgact ggacgaattt ctactataca tacggacttc tttctggtta cggcggctat
     9661 gtattcggtg aaaatggtac aaatccaaaa gacatcggac ttgctaatga aggtgccatc
     9721 aaggcaattg aatacgctaa aacttggtac gaaaaatggc ctcaaggtct tcaagacggt
     9781 actgctgcaa acaacttgat taacactcaa tttacagacg gtaaagcagc tgctatcatc
     9841 gaaggtcctt ggaaagcagc ttcatataaa gaagctggtg taaactatgg tgtagcaaca
     9901 ataccaactc ttgtaaacgg caaaaactac tcagcattcg gtggtggtaa agcatgggtt
     9961 gttccagcag gtgctaaaaa ccaagaaacg gctcaaaagt ttgttgactt cttgactgct
    10021 acagaccaac aaaaagcact ttacgacgct acaaacgaag ttccagctaa cactgaagct
    10081 cgtgaatatg ctgtaagcaa aaaagacgaa ttgacaactg cagttatcaa ccagtttgct
    10141 tcagctcaac caatgccaaa catctctgaa atgggttcag tttggacacc agctggtaac
    10201 atgctcttcg aagctgcaag tggttcaaaa gacgctaaga ctgctgcaac agacgctgtg
    10261 aaagctattg cagacgaaat tgctcaaaaa cacagcaact agtcaagttt aaagttggac
    10321 tgggagttct cttcccagtc tactttatta cttaacgata aactgaatag atatagtctt
    10381 ttagtttact tttagtacga ggcaatgagc cgcaggcata actgaagtta ggcaaggcga
    10441 gttaacgaag taataaaaga aaaactaaat gacgataaca tccaccgtct attcagaagc
    10501 tatataagga gactatgact atgaaacaaa atccaggtaa ggcactcctt ttgtccctga
    10561 tccctggtct cggacaaatc tacaataaac aaaaagccaa aggatacatc tttttaggtg
    10621 ttacgcttgc cttccttgtt tattttattg ctattgcagc tggtgaatta ggcaacttga
    10681 ttaccctcgg tagtgttcgt ggacaagata attcgctatt catgctgatt cgtggttctt
    10741 tccacttaat tatcacagtt gtctatctcg ctttctacgc actcaaccta aaagacgcac
    10801 gtgataccgc gaaacgatgg aattcaggga tgccagtggc taccacatta aaagaaatgg
    10861 taaaaggtat ctatgagaat ggtttcccat atctcttgat tatcccatca tatattgcga
    10921 tgacctttgc tatcatcttc ccagttgtcg ttacattgtt tatcgccttt acaaactacg
    10981 acttcaaaca cctgccacca agcgcattgt tggattggat tggtattacc aactttacaa
    11041 acatctggaa actaagcaca ttccgtaccg catttggttc tgtacttggc tggacaatta
    11101 tctgggcact ctgcgcttca acagctcaaa ttgttatcgg tatcttgaca gccatcatcg
    11161 caaatcaacc attcatcaaa ggaaaacgta tcttcggtgt taccttcttg ctcccatggg
    11221 ctgttcctgc cttcgtaact atcttgactt ttagtaacat gttcaacgat agtgtcggcg
    11281 ctattaacac acaggtattg ccattcctga gcaaattcct accattcatc tataatttcc
    11341 ttatcccttg gaagaccgac ccattctgga ctaaagttgc tctcattatg atgcaggctt
    11401 ggcttggttt cccttatatc tatgttttga ccttaggtat tttgcaatcc attccaaacg
    11461 acttgtatga agctgcttac attgatggtg caagtgccat tcaaaaattc cgtaacatca
    11521 cccttccgat gattttagca gtagcagctc caacactaat cagtcaatac accttcaact
    11581 tcaacaactt ctctatcatc tacctcttca acaatggtgg tcctggtagc gtcggtggtg
    11641 gagcaggttc aactgacatc ttgatttcat ggatttataa attaaccaca ggtacatcac
    11701 ctcaatactc aatggcggca gcagttacat tgattatctc aatgattgtc atcagtattt
    11761 ccatgattgc cttcaaaaaa ctaaatgctt ttgaaatgga ggatgtataa gatgaaagta
    11821 tctgtaaaaa ctagacgcat acttaatcag accttaacct atctctatct gattgttctg
    11881 tctgttatca tcatctatcc tctcttgatt acaatcatgt ccgccttcaa gtctggtaac
    11941 gtggttgcct tcaaattgga tagcaatatc aactttacac tagacaattt tagcaagctc
    12001 tttagcgaga ctctatacgg aacctggtac ctcaatacgc ttatcattgc tctcttgaca
    12061 atggttattc aaacaagtat agtggtacta gcgggttacg cttatagtcg ctataacttc
    12121 atcgctcgta agcagagttt ggtcttcttc ttgattatcc aaatggtacc aaccatggct
    12181 gctcttaccg ccttcttcgt tatggccctc atgctcaatg cgctcaacca aagctggttc
    12241 ctcatcttcc tttatgtcgg tggtggtatc ccaatgaacg cttggctcat gaaaggctac
    12301 ttcgatacgg tgccaatctc acttgatgaa tctgcaaaac tagacggtgc cggtcacttc
    12361 agacgtttct ggcaaatcgt cctcccactc gttcgtccaa tgattgctgt acaagcgctc
    12421 tgggccttca tgggaccttt tggagattac atcctatcca aattcttgct tcgtgaaaaa
    12481 gaattctata ctgtagctgt cggacttcaa accttcatca gtgatgtgaa aaatatgaag
    12541 attgcctact ttgcggcagg tgccattctt attgccctcc ctatcagtat cctattcttc
    12601 ttcctacaaa agaactttgt atctggttta acaagcggtg gcgacaaggg ataaccctgt
    12661 cccacctttt ccctttttag gggaaacatt gatatttatg aaaggtccaa cgaatgctcc
    12721 cttatccttt ttcctatttt tctagtatct tctctccaag aaatatgttt gccaatcgta
    12781 gactaatgaa cctttggcaa cggttgttta caacgctttt tttagtggcg ctcttgctca
    12841 ttccgagctc cctacagact gtcaatctcg aaacctatcc tttagaaaat ttcgtagaag
    12901 gtatctatgc acctctgaca gatcaggtcg tagatgacct ccgtcaacat gcagtcatcg
    12961 aaaacaacca cctaacctat actggaaata ctaactggga gcaagtgaca tttggtgata
    13021 ccgttagctc tagttctgac tttacttatc aattcgccaa tcagtctctg actattcgta
    13081 aaggaagtga tattctaagc gaattgactt atgaaggttt gaatactgac gattttgcta
    13141 gtaaggaggc attaactgca gccatctcta aaaaatggta tcaagccaac cgagcagctg
    13201 tcggactgtc catcactctt atttcgacct ttattttaac aacaaaccta ctcttcattc
    13261 tactaggagc cagcttcttc ctctttttga ctaaaaagtc tcgtttcttc cattttgaaa
    13321 cgtttgcaga gtgctataat tttgctatca actgtctggg cctgcctact cttatagcct
    13381 gtctaattgg actatttggt caacctatga ctacccttct gaccatacaa aacatcctat
    13441 ttgtgctatg cttgttatgg gtatttttca aaacaaaatt cagagatgaa atctaggagg
    13501 tctatatgcg tgctaccatc aaagatgtgg ctaaattagc tggtgtctcg ccatctacag
    13561 ttactcgtgt cattcaaaat agctctgcca ttagccaaaa aacgaaagac ttggttagaa
    13621 aagccatggc ggatctcaac taccatccaa acctcaacgc tcgaagccta gtttctagct
    13681 atacacaggt tatcggactg gttttgccag atgactcgga tgttttctat caaaacccct
    13741 tcttccctac tgccttacga ggaatttctc aagttgcggc agatcataat tatgccatcc
    13801 aaatctccac aggaaagaat gaagaacaac gtctagaagc aatttctcag atggtctacg
    13861 gcaaacgagt ggacggcctc atcttcctct attccaagcc cgacgatcca ttggtacaat
    13921 tagctatcca acacaaattc cccttcctca ttcttggtaa ggcggattct ccattcatat
    13981 cactagttga taatgataat atccaggccg gctttgaagc taccaactac ttcatcaata
    14041 aaggttacaa aaacattgcc ttcgtcgctg gtaacaaaga gttggtcgta tctcaagacc
    14101 gttacacagg ttacaagaac gcccttaaat cgcacaatat tcccctagat gaaaacaagg
    14161 tcaagtttgt gtctggcttc cttctcgaag acagtgccta caaaatttct aagaaattac
    14221 tcaaacaaga cctggatgca atcgtcacaa ccgacacttc cgttgccgaa ggtgttgtca
    14281 aatatctcaa cgaagtcgga gtcaaacttc ctatcatttc ttttgactcc gtcaagccaa
    14341 aactagatat tgaagcctac gttgacgtcc acgctattaa actaggacgc gtcgcattca
    14401 acactctgca ccaaatcatc aatgacaaca aggaagacaa gcaagtctgc taccgacgtg
    14461 tcattccaca taccattacc gaactctaac ttagaaagga gccttttggc tcatggcatt
    14521 acgacaattt caagcatctc ttgacgatgt agcaactatt cgcttggtca tggaaaaacg
    14581 attcgactcc gaacatatga gcttctcact tgaatcaaat gatgctacat ctcaactttt
    14641 tatccattcc tgtctagaag tagataatct cgtcatctat tatctaacca gcctccatgc
    14701 actgacattg gacaaggact ataccatcta cgaccaagac cgtaataaaa cagaactcgg
    14761 ctatggtcat atcgttcgct ctacactttt tgaacaaagt tatacataca ataggcaaga
    14821 cttgggagca acctatcgac cagaggcaac aacctttaag ttatgggcac ctatttctaa
    14881 gcaagttttt cttattctag aaggaaatcc acatgcaatg acgaagggag caaaaggagt
    14941 ttgggaaatt actgttgaag gagacctaga agcgcacagc tatcactatc tccacaaagt
    15001 aaacggtgaa tggatttccg ttcacgatcc ctacgccctg tcttccgcag ccaactcagg
    15061 tgacagctac gttatcaatc cagataaact tcataaaatt cgtcgtgcta agacccaacg
    15121 acctatctct caggccatta tctatgaaat gagcgtccgt gatttctcat ggcaaaaaga
    15181 agctggtttc aagcatcgca gtcaatttct cggattgaca gagtctccta ccatggatgg
    15241 aatgaagcta ggaatggatt acatcagaga actaggcgtt acccatattc aattattgcc
    15301 tgtttatgat tttggcagtg tggatgaaaa taaaccccaa gctgtttaca actggggcta
    15361 cgaccctatg caatacaatc tgccagaagg ttcctattct agccaaccaa acgatcctta
    15421 cgcacgtatt ctagagctac aagaagctat ccaggcctac catgatgcag atatttcagt
    15481 tattatggac gttgtttaca accacgtcta tcatgctgaa aagtacgcat ttgagctcat
    15541 cgttccaggc tacttctatc gctatgatga ccacggcatg cgaacagacg ggacattctg
    15601 cggtaacgac gttgccagcg agcgcagtat ggtccgcaac tacatcaaac aatcccttcg
    15661 ccaatggcta gaactctatg gttttgatgg cttccgtttc gaccttatgg gtattttaga
    15721 tagcgaaaca atcaaccaga ttcaaactga attatctgcc attcacccaa atgtctacct
    15781 ctatggtgaa ggatggaaaa tggcaacagg cctcgaattt gaaaagctgg ctcaccagta
    15841 caatgcagag cagttacctg aaatcagctt cttcaatgac gattaccgtg atacctttaa
    15901 gaaactcttg ctcaatccaa accgtctagt agataagcaa ttgcacgaaa aggtacaaca
    15961 tctcctgact ggtagccgat taagccattt cttaacgcct caacagtccc tcaactacat
    16021 cgaatgccat gataatgcta cagcatttga ctatttccat attgagaatc caaactggac
    16081 acctcaacag cagaaacgtg ctgcaagttt tggtctccag cttatcttaa tctctcaggg
    16141 tatggccttc attcacagcg gacaggaatt tttccgtaca aaagatgaaa tagataatac
    16201 ttacaatgtc cctgaccgta tcaaccgtct ggactggaca cgagctattc gttataagga
    16261 acatatcgag ttcattcagg aattaattac cttccgcaaa aataatccaa tactcagcca
    16321 ggataactac gaaagtatcc aagaaacttg tgatttctac tggttaactg aatatgttct
    16381 tcgctatcaa gtgacaagtg aagaaaagac cattcaattt atcatcaact ttgcggatag
    16441 cgactttacc tatgaacgtg aaaaagataa ggcagttctc tacaactatc ctccagtcga
    16501 agagcgtgat cagcaagaaa tcacgattgc aggacagagt atttgcattt tagaagacaa
    16561 ataggacttt agaccgatga aacacgacat agtctgaggt atactatagt caatcctaaa
    16621 tatttttcat aatctcctta aaactcactg gtcacgctgg tgagtttttt ttgtgtgacc
    16681 aaaaaaacaa cccagcataa agctgagttg aggctcttta catcaattcc attatcttcc
    16741 gaccaatgcg ttcaatgtca ttctgcatcc ctctgagttc aaaggtgtct ataacaggaa
    16801 aatcaaatcg ctcagcatat tgcttggcag tcagacaata ttggttatta aaattgcggt
    16861 tacctgagcc aacgacaccc agacaaagct ctgcattggt ccccaaagcg ataaaatctc
    16921 cgagaggatt ggtcagaatt tccacatctc cattgtccac tccatttcca ccttctaagt
    16981 aggttggtaa aaaggcgaca aagggtgcat ctagctgata gaaaggaata tcttgcttga
    17041 ccaaatcttt gacatgaact agctcaatct cccagtcagt ttgaaattgg aaaaaagact
    17101 ttagacgttt cacaaaactc tctgtattgc cactcaaact gatgtaaacc aaatatattt
    17161 tcttcttcat acatttcctc tttataataa aaacactaca tgtagactgt aaatactatt
    17221 ttactcctac atgtagtgtt tgtcaatcag tcactttcca caaaccgacc aataatattg
    17281 acgttgtcag ctaccgatac aaaggctttg gggtctgcct ttttcataat atgcttgaaa
    17341 tcattaaact ctgcacgagt aatgatggta atgagaatcg tcttttgctc atggttataa
    17401 gcaccctcag caccgttgat aatggtcaca ccgcgattga gcttcttatg gattttctta
    17461 atgactcgtt caggatgatt ggtaataatc atggcctgca tccgtttttg tttcacgaaa
    17521 atcgcatctg tcatgcggct ggagataaac agcgcaacca ttgaatacag ggcatattgc
    17581 cagccaaagg tcatccctgc aatcaacata atcataatgt tgacaatcaa ggaaatagag
    17641 ccaacttgac gtcccgtctt cttgcgaata atgatagaga caatatcagt accaccactg
    17701 gatacattgt tcctaagtgc aaagccaatc ccagttccca gcataagacc accgaaaata
    17761 gcatttacta acggatccgt cgtcagggta actggcggca taaattggat aaagaaagaa
    17821 ctgaaggata ccgtaataaa ggtaaagata gtaaatttat aaccaatttt ataccaagct
    17881 aagataagca agggaatgtt cagaccgtag tagaccaccg caatcggtac attccaacca
    17941 gcgtaagaag ctaccagagc agaaacaatc tgtgcaagac cagtaactcc agacgaataa
    18001 acattccctg gtcgaaagaa aaaattgact gcaatactag ataggagtcc ataaataatg
    18061 gaccccgata tccgttcagc atatttttcc cgagaaattc ccaaaatgac cgaccaaaac
    18121 ttattctttc tggcctttct taaaaagcgt acacgctgtt ttctgaaaaa tttattcatt
    18181 ttcaagtgta atgtttaaag ccaattcctc taattgtgct gatgcaactg tgctcggtgc
    18241 ctgtgtcatc ggatcagatg ccttgttatt cttcggaaag gcaattactt cacgaatgtt
    18301 atcctcacca gccaataaca tgacaaagcg gtccaaaccg atagccaatc caccatgtgg
    18361 tgggaagccg tagtccatag cttctagcaa gaaaccaaac tgctcatggg catcctcagc
    18421 agagaagcca agcgctttga acatccgttc ttgcatgtct ttttggttga tacgaaggct
    18481 accgccaccc aattcataac cattcaaaac aatatcgtaa gcaactgcac gcacctgcgc
    18541 caaatcacca tccaaatgat gatctgtttc ttccgtcggc aaggtgaatg ggtggtgggc
    18601 actcatgtag cgaccttcct cttcagacca ctcaaacatt ggccagtcta caatccagag
    18661 gaagttgaac ttgctttcgt caatcaagcc ttgctctttt gccaagcggc tacgcaaagc
    18721 tcccagggta ttattggcta cttccaattc atccgccaca aagaggacca ggtccttgtc
    18781 ttcaagttgt aaacttgctg tcaagttttc tgtaataccc gtcaagaact tagcaactgg
    18841 gcccgctaat tctcccttgt ctactttaac ccaagcaagt cccttagcac caaattgttt
    18901 tgcgtagtct gttaatttat caatgtcttt acgtgagtag ctatccgccg cacccttgac
    18961 cacaatggct ttgacaactg gtgcttctga gaagaccttg aagtcaactt ccttgacaag
    19021 ttctgtcaag tcttgcaaaa gcatctcaaa acgagtatcc ggcttgtctg aaccatagaa
    19081 gttcatagca tggtcatagg ccatacgagg gaatggcaag gtcacatcga ttcctttggt
    19141 atctttcaag accttagcaa tcaagccttc tacgatgtct tggatttcaa cctcattcaa
    19201 gaatgaggtt tccaagtcca cctgtgtaaa ctcaggttga cggtccccac gcaaatcttc
    19261 atcacggaaa cacttaacga tttggtagta acggtcaaaa ccagcgttca ttaaaagctg
    19321 cttagtaatc tgtggacttt gtggaagggc ataaaaatgg ccttttgaca cacgagaagg
    19381 taccaaataa tcacgcgcac cttctggtgt tgacttagtc aacattggtg tttccacatc
    19441 aataaattcc aagtcatcca gataattgcg aatgctgtgg gttacagccg cacgtaattt
    19501 gaagttgttg agcatttctg gacggcgaag atccaaataa cggtaacgaa gacgagtgtc
    19561 atcgctggct tcaatgccat ccttgatttc aaatggagtt gtcttagccg tgttcaacac
    19621 tgtcaagctg gttacttgca gttcaacagc tcccgtcggg atattgtcgt tagcttgctc
    19681 acgctccaca accgttcctg ttacttcgat aacaaactca gaacgaagac tctccgcttt
    19741 tgccatcact tcagcttcaa ctgactcagg gttaatcacc aattgcataa tgccttcacg
    19801 gtcacgcaag tcaatgaaaa tcaggccacc caagtcacga cggcggccaa cccaaccttt
    19861 caaagtaatt tcctgtccga catgttcctt acgaacacgt ccagcgtaca tagaacgttt
    19921 cattttcttc ctcttttact gattttatac ctattcattc taccataaaa tagctgattt
    19981 ttgggagaaa ttgccgagaa aaatcgaaaa accagcccta gttgactggt ttacggtata
    20041 acatatccaa atgtgggata tcatcctcca aatatacttc agaaataggt tcgaagccaa
    20101 gcgattgata gaatttttcc aaataggctt gggccccgat tttaatctga ctttccttcc
    20161 actcggttgt aatgtaagcg atagcttgct ccatcattgg ccgacccaat cctttaccac
    20221 gatggcttgg cttgatgacc acacgaccga tggaaacttc atcataactt agaccagctg
    20281 gcaatattct caaataagcc actaactccc cattgtctgt tcctaaaaga tgaagacagt
    20341 ttggatcctt tccatctatt tctggatagg gacaggcttg ttccacgaca aacacatcgg
    20401 tccgaagtgt caagatagtg taaagttctt gtaaactaag ttgttcaaat gtttttattt
    20461 cccacttcat acattcctcc tagggcaaaa tgttccctgc acttctgtaa atctcgtacc
    20521 attctggtct cgttaaggta atctggctag cctgggcaat ctttgtcagg cgatctggat
    20581 tcatagaacc tacaatcgtt tgaatctttg ctggatgacg caaaatccaa gctacgacaa
    20641 ttgtttcatg tgaaacattg tatcgctctg ctatttcccc aatagtttga tttaaatgag
    20701 catagtcagg atggttggca aaaatacctc gttgcaaatc aatcaagaaa ggagaccaag
    20761 cttggatagt gattttttta agctggcaat aatcaatcag tccaccatcc cgcatagtcg
    20821 cagcatcatc tttcatattg acatgtaatc cagcatcaat caaaggcgtg tgagcaggag
    20881 atagctgcaa ttgattaact gcaagcggtt gattgagata ggattgcaat aactccattt
    20941 ggtgaatgtt ttggttgctg acgccaaaat gatgaacctt ccctactttt ttcaaaagat
    21001 aaaaggcttc tgcaacttcc tctgcttcca ttagagcatc cggacggtgt aaggcgagaa
    21061 catctagata ttctgtatcc aagcgcttga gaataccatc cacagattcc aaaatatact
    21121 cttttgagaa atcaaaatag cctttacgaa tgccacattt tgattggaga agcaggtcct
    21181 cacgtttcaa tcccgccaat tttgccccct gagcaaaacg gatttctgac tcaccaccac
    21241 cataaatatc cgcatggtcg aaaaagttga taccttgttc aaccactgtt ttcaagacct
    21301 gggctgtttc ctgcccactc aaacttgcca ttcgcataca gcctaaagca attcgtgaag
    21361 cttctagacc tgtttgtcct aatctttgca tttattttct cccttgtaaa cgcttttcat
    21421 attccttatt ataacagaaa aactcagccg aaactgagtt ttagttaaaa ccataaattc
    21481 ttttcgcgat tttatagacg gcattggaaa tttttcgacc tgggtaaccg taaaggttgg
    21541 ttaattgccc gagtaaccct tttcgaacaa ttttaaatag agctggatta ttatcacgaa
    21601 tatagtgcca taattcctgc tttttcatca tgtgttcagc tgttccagca cgattgagaa
    21661 gggcacagga gataatggtc gtaatctcca catgattgag caaataactt cgtaaatcag
    21721 gattgtcaat ggcctccaag tctaaattat ctaccaaaat tcgattgact tttaactgct
    21781 ggtcaatgca cttaatcatg acggattcat tgacagactg gtcatttcgc ccaataaagt
    21841 aacgataaaa atctactggc agatagaaca tcgttttcac agcctgcaaa ggagtaaata
    21901 caaaaatatt atccacataa aaagtatgct ctggcagaac aagaccaacc tcacgcaata
    21961 aatctgtcct gtaaatcagg gaatgcatca tcatgtattg ccccttggaa aaggcaccta
    22021 catcctccca accgaagata cggttctcag gtagagtgga ttgataagac atggattttt
    22081 tgcgagattg tccctctttt tcgtaaacaa agttggagat aaaggcatca actggcgtat
    22141 tttcatcttc caaagcctgc aaactctcta ggatcttcaa ataggccctg gtatcgaccc
    22201 agtcatctga atctacaact ttaaagtact tccccttggc ctcacgtatc cctgtattga
    22261 cagctccgcc atgaccttta ttttcttgat aaatagcccg tacatttgaa aattgattgg
    22321 caagctcctc tgcgatttcc tgagttcgat cggtcgaacc atcgttaata atcagaattt
    22381 caaccttgtc cccacccatt acaagggaat gaacacagta atgaaggtag gcctccgaat
    22441 tataactcgg aatagcaatg cttagtaagg tcatctttct ctccatttga cacaatttgt
    22501 tactatttta tcatattttc aggaaagttg ctgagtattt ttgtttgtaa aaacagacct
    22561 gctcggatag ttactacggt ataacttatt cacaacttgt ggataagtcg tggatttttc
    22621 aacaatctgt ggaaaactat tgggcaaaat ctttttttgt tttaatcact attttctcca
    22681 catctcgact ttgactccgt tttataatca catcaagata atcagccaag aaacttctcg
    22741 gtgatactgc ttgcaaggtc tgataagcct ggcttgaaaa taccttctca tctagccaag
    22801 tctgatctag tttttctcct gaaaaataag cacggttggc cggcatgata aattgtgaaa
    22861 tagcatctgc cgtctcgccg tcataccaac gctcatcgtt taacacattg tggaccatga
    22921 tgtcactagt attgtcaaag acagtttgta agtaggtaac gtgatcctgc aaatttggat
    22981 cactaggttg gtgcttttct gtccacgaag ccatatcaag ttgagttttt tgatagtgaa
    23041 tgcttgtatc atcaaggctc aattggccga taatagctgg ataaatagaa aatgctccat
    23101 tgaccacctc tgtatagatc acattcccca agtcttcctt aacaatattc tgagaatgag
    23161 tgtgccctga aaaaccaaat tgaatgccat attgattgaa caaggtcttc aaatcggcag
    23221 cattatccaa agtatagcct tttgagagca tggcatgctg ttgcaaaaca ttgtgatgga
    23281 caacaggtat caggctcact ccagcttcct tagctgacct aagctgaatt tctatccatt
    23341 ctagagtctc ttttttgata cgtccattgg ttggaggagc tccttttccg tagccgtcag
    23401 agtagatatt gctgtctatc atcagaaacc aagcatttga aaatggtttg accaggtagc
    23461 tcaatgaggc tttgtcacga ctgctagctt gttgataacc gtgatgggca aatagctctg
    23521 caaattgctg agcggtgacc tgatccgtca ctatctgctg gtctccctta aaggccctag
    23581 cccatccact ggcaatatca tggttgccag gaatcaccaa aacctccgtt cctttttctt
    23641 caatccgggt aaaatattgc gccaactcaa ccatgctctg cttttcaccg tttagagtca
    23701 aatccccact gactagtaat aaggacggtt gctcacgctc aacctgctca accaaagcct
    23761 ccatccgctc ttttccataa cgtaaatcct tgccagcagc tgttttttct atataggaaa
    23821 atgcctctcc atcatcaaat aaatcctgtg acaaataatg caaatcagtg ataatccaga
    23881 ttttatcctg atctcttaat tcagcctcga cataggattg ctgacgctga ctagcaatag
    23941 tagaccaaat caaaacaatg actgcaatag ctcctaacaa taatactatt ttcttcttca
    24001 taccttaaaa gtatatcatc ttagagatag agggacaaga aaaagaagct cgaccttctt
    24061 tctgccgagc ctcttgtatc tacaatttat ttcttagcaa atcctgctgc ttgttcacca
    24121 gcctgacgac caaatacgat gatgtctgct actgcattac caccaatacg gttttgacca
    24181 tgaacaccac cagttacttc accagcagca taaagacctg aaattgctga accatctgtt
    24241 ttcaatactt cagtattggt attgattttc acaccaccca ttgtatggtg aataccaggt
    24301 gcaactttaa tggcatggta agaaccagta ttcaaagcat ggtccatacc tgatgtacga
    24361 ccaaatgccg catcattttt gtctgccaca gccttgttcc aagtatcaag tgttgtttga
    24421 agagcatccg caggtacacc aatttcttta gctaaatctg caagtgtagc acccgttttt
    24481 acaagacctt tttcttcgta ttgagcaatt gctttcacac gatctttcaa ggcatcatca
    24541 aagataacat aggcgtagtt tggatccaaa ccattgatag ccgcagaaac tttatcacga
    24601 gtttccaatt cgttaacaaa acgctcacct tttgagttga caaggatagc accttcacca
    24661 cgaactgctt ctgtaatcaa gtaagaagtt tcttgctcaa cacttgggtg aatttggatt
    24721 tgttccatat caacagttgc tgcaccttct gattcagcaa gcacaatacc atcacctgtt
    24781 gaaccttctt ggttagttgt aacataacct ttcaatgctg gatttaattt agcaaccatt
    24841 tccaaatctg caccgaaacc accagtcgcc aatacaactg ctttagctga gattttcttc
    24901 gtttcaccag caatcttcac ttcaacacct gtcacagccc catccttgtc ttggagtttt
    24961 gtaacatctg cattgacaaa aattggtact tcacgttcaa caagattgtg gtaaagacca
    25021 tttaccaagt agccaccgac agctgaacca tctgcaggac ggtgtgtacg tttttcgctc
    25081 ataccaccag ttgtagttag gttgctaaga gtgatcccca ttccatccaa ccagtcaatt
    25141 gcactagctg agttatctac aagataacga agtaattcag gatcgtttgt ccctttacca
    25201 cctttaagcg tttcttcgta gaatttatcg tttgaatcag caataccttc cgcttcttgg
    25261 aatttggttt gtgaagcatt cataccagct gatgatttag ccgtattacc accgataaca
    25321 ggcattttct ccaagagagc aacactagca ccagcgtctt tagctgagat agctgccgac
    25381 atacctgcac cacctgaacc aacaacgata acatcgtagc tatctttcaa ctcagttgga
    25441 tctgtataaa ctttctcaga tgcacctgaa acagcttctt ctgatgaaga agaagtcgta
    25501 gatgatgaag atgtagctgt cttttcagac ttacctccac acgccactaa aaacaaaaca
    25561 gcaagtagac tcatcaacaa gcctaaccaa gctttctttt tcatataaac ctccaatttt
    25621 tatctgatac tagtattata caagattgtg aaatgttttt caatctgttt tgaaatattt
    25681 ttcacaaaca taaaaatgta tgaaattcac ttcatacatt cttaaaacta tttactgccc
    25741 ttgaagagct aaaaaattct ctatttcgga tagcgttgct tgactccctt tacgaagtat
    25801 tctagataat ttacccgatt tataataggt taagctagga accgtagcaa gaccttgact
    25861 ctgtcgaaat tgttgtattt ttccattcgg atccgaatca ctatctaaat agtagatcgt
    25921 cgcatgtgat tgctctactg cctctgttag ctctccatct ttatccaacg tattcttagc
    25981 catacctatg ccacgtagtt tgttccactt agggtggcgt tcagataacc acttaacatc
    26041 atatgaaacc caataatcac gaacttcaat ttgactatat gctttctcaa gcgttcgata
    26101 atgttggcct tgcttagcta gcttagccag taattttaca tcgttaaaat aaaggctaat
    26161 atcctcataa agacttcctt gattgccctt taatgcgaga caataatctc ccttaccctt
    26221 gatgatttca tctactatct ccgcttgcgt ccctatcgca tctatggtca caatcccttt
    26281 tctaatgtca ataagtcgta acaaacgtgg aatagccgtg atttcattac ttttctcttc
    26341 aactacaacc tgacctagac taatacgatt atccccatca taagccgtca caatatgatt
    26401 cggacgttgt gttttactac catttcctcg catagtctta ccatctatag ctattaagcg
    26461 ttcaacagta gcactttctt gagtctgact aaagtcaact tttaactccg ttaaaaattg
    26521 aggattaagc atactcacta cccgttctaa cgtatcatga gaaggacaac catcactcaa
    26581 atccacatat tgtcctagaa gggattcatt catctcaata aaatcttcca tttctttcca
    26641 agtttcaata cctgccagct gactaacaaa aacaacaaac aaaatggtag atagggaata
    26701 acgaactttc cacggtcggc gtttatctaa ttcttcactt ttggcttcta gtgaaacaag
    26761 aaaatcaatc atcgtagtta tctctatctc tagtttacta ctttgattga ttttgtcgct
    26821 atgtaaatag ttttctgaaa ac
//
LOCUS       DATUCG010000014        26728 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.014, whole genome shotgun sequence.
ACCESSION   DATUCG010000014 DATUCG010000000
VERSION     DATUCG010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26728
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.014"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            245..8665
                     /locus_tag="VCA20_000495"
     CDS             245..8665
                     /locus_tag="VCA20_000495"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012956.1,HMM:NF037639.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEP1834353.1"
                     /translation="MKKVRYFLSSFLLLVLCLDIIRLPITIFSESVSATTPLTIRTKY
                     LEHLKEDGQKLAIKLDTSQKQLDRLDVIVQIEEGVDISKGLSLVLQNELELESQLDEQ
                     GMLKEKLPSGFVQDEDYREEDVPKEFPEQEVVPTVATQDSLEAPLPVYGLGYYSIKIK
                     PEPERHIEWKVDEEQPERRYEEILLRLKIKNPLQTGQFMAVLVSDYKTILTEDWKTFE
                     ELVNRNSIEMVTDSAAFDTSQPVSEKSVSDTEQPIPETSESISDNTQSNPVPEGEEVI
                     QEEQALAIQTANLRLVLREHEEGVLQPSKPIAGAEFSMIAVGNPENAHKGRTDEQGVL
                     EWRDLPVGEYQISQLSTAQGYEVEKQLPVIQLQESGVEKIIHVTNTKKNELEGQIGSM
                     LRSARTRRAATETNTDRTTTIINNPNGTTTTITKEEEITKDIFDPIRIEIPGVTRHFR
                     QGGDLRKKEIQVTSTGEKKIIWEYITTVTGASDRYIKQIANTFMVPVDSGLGTPKITS
                     ISYNQGQTIENPETSRYSDSGRFNSVTNQLPIQDGTYVYTIETPILLPSENYTLDYYS
                     DVKVQAPIRSRLTQNGQTIVLTREEERNIKSAGSLTLPSDNTTSPYDTPKLSDTIRVN
                     GRYRESDNKVIEWYTSRLNTTDSVQTYTFDVAEDKNTQAVHSKIVYVYEPTDGGNYKL
                     KSRAQVDKTQSQITVNDVPVGGIAVVETITNVTNEKANHTITGASLEALKGDITIQKK
                     WATGTEKTAVTYTINGGSYNNHKVTLQANQSQVIIPNVEKFSGTGSTASKTRIYYEVT
                     ENVPDDYALSTAQMDWKNLQYVFTNKKDTSQPITPTPPGSCESYGVTHVEPVNINWLT
                     WQRGSNRWGGFHGSLKFYFKLPAFAKEGDSFTIELPRELKLEHLANPSVEWTPVYKSG
                     TSEILARTYHVEDRKLKFVLTSAAYSVTEYSGWFEIGSDQSANLVQINNDKNVAGKKY
                     LTGVRPEVPVWYSFDPENPINTQSNIVNKKLKYQTSYKGGHFDCSHTVVGDGTLNATE
                     NGSGGLQGSLRQFSSVHKQAINQTADSVTWELVYNGGRNDISGSILAIRRKFWDRIHV
                     DTPLYDGNTHTSILKDLEIFVSDGTPAVSYVESEMVKLKYRTTSNSGENTTYHYGIDG
                     TDMGLAVLLKRENWGDNSFGFQSNYALEFYYEGRDWGRKAIIVRIKQRKNGNTTLRNA
                     VNVNWNSLVISGKMHTNNYFVAGDTVASGGAYPTDWYGFRLKKVDQQNRPLEGAVFTL
                     YDSQQRPYQTIRSNEQGVLEFTNILGGEYTFKEVEAPAGYTLDTTVHRLIIRNSENIT
                     IDGQSYTAQAPFEIINKKDAKLSLKLKKKDLANTPLKGAVFRLRKTDGGQPYDETLGA
                     TFESDEFLFNELTVGNYTLEEVRAPTGYRGMGQINLTVYEENGTLKLRKQADTANSQT
                     STPQLTNGVVTLDIYNQPLELTFTKQNAQGEPLENAIFELRQIHSTGFTVVRTNLFSK
                     ANGQFVLKGLLPNTTYELWETRAPDGYAKPTQAVVQFTVNEDGTAHFTRGSSTIVNSP
                     PKFKVKIVKIDAETGQPITEGVNDTAPRFTVTNATGDTLNGQIANLQNGMYTFTNGSG
                     TNSGFEAGTYYLKEIRAPQGYTRLTNLIPFTIHSDGRVTVSEEFADGIATSATSPDTV
                     QIKVKNDRLKTLRISKRIKGLENLPSLITGQMTFTLTKDNDATAVSAVQTHAATEDFV
                     FNNLTYGSYTLVETAPPVGYMVEPIVYKVAVSRAGVSLYRTSDQVTPSRVEGASLITS
                     PDLSNIQSGPESNAIDGSETTSVTYHNFNGQGNTIPVGAYLGVDLQSVKRVRNIFYLQ
                     GNQNNVTDRLNRYTVEYSIDGETYYPIASSATAERFEQSTDLLARYIRVRNEEQVVNK
                     WYAVRELRISAQEAESVSLQGGQAFPIGNIQHPEIQIEKQDMNNRIIQQAVTFKLYKV
                     PDDTTSQTVTGTLTDQNLVQTFTLTNGQVRQTLEAKVQGRYALVEGTPPTGYRGLASP
                     ILLDLQTLQQPHSGSQMKTTRFSLVGTNDKVRIDTNTANVLKFIVKNEPLTYHLKILK
                     RDVNNAATGLDAHFELYDATGTTVIERGNTSRDGFSLTFPNLSPNSYVLKETEAPANY
                     NPIAPIHLTIDQTGQLTIQSGPSDLIAIQSADDSHLIQLTIKNRPFTPISIQKVDKRS
                     NETTLTGVRLRIQAKNGAQMPQFSDRSWHETHYQGRVIDSEKALEWNSSNAGNAVFKL
                     PQGTYTISELSAPAGYEQLQPFDITVAEDGSVQLGQGTNTEQVSQGQKDGRINIKLTN
                     VFKPKIKITKVDSRDANRKLAGARFKLFGPDENTQIGQEVTTGSNGEITLPGLEPGTY
                     YLQESQPPTGYQANTRKYQLTIGNDGATQLVNGDAKIKIGNSQDQGADKLIPITVKND
                     RTPFTVKIRKRDYNDVNRGLNARFQLYRKNGNQEELLSGLVGDTIVNNHELVLPNILP
                     GTYILKEIRVPSGNYEAIQPIQFTITEEGRLQIDVNDRDFASVENGGSSQELVLLIKN
                     IKKFRLRIKKQDATNDQTLLDGARFTLYRAQGNTGGKLEQLATGVTANGLADIDVGPG
                     YFILQEIQAPTGYVLNGKEYIFQINHDGTVALHNSDDMTSLQGADNNRVVVFTMKNRR
                     KTGQFKLAKRAYSDSDRRLAAVFELKEENGATPVATKTTATDGEEIVFDNLRIGQIYQ
                     LKETQAPEGYQLNTKVYRLRLTDSGQVELLDGDDLLSLDDSNRQLLTAKNLKKGEYPK
                     TGGFGVLPYITLGGGMMLLALAVELGKEKCWKRIRK"
     gene            8692..10566
                     /locus_tag="VCA20_000496"
     CDS             8692..10566
                     /locus_tag="VCA20_000496"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012948.1,HMM:NF027869.1,HMM:NF027884.1,
                     HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HEP1834354.1"
                     /translation="MKKLTKLFSLLTVLLTMFGPLGNLRHLVHANDAQPHRTAVVVHK
                     VLMTPEQLENFDHDQKQVTNKYVGNQIQNFGEYFGEGSKEIEGVNFKVWTKVDRAEDG
                     VTKTGIELGIAEDNNNYKLNQSYPDGVVTLADTQGATFELEDGTYIFVEDKENSPYYN
                     KDGVTTAKDASKELTQSKAVPFKLELPVTKPDGQGYFDLQTKLHVYPKNTEDKPDVKK
                     TFGDGNTGVKEYDVGTKIPYKITTRIPKGSTYRTLIWDDLMVNGLDFDVTTGVQIVSK
                     EVQDFNNRHYSVEKDIRGFVLKVNQEGLNLIEEAAKTQDVNFTLTYNGVLNTSAQINT
                     QIPNKVTFHFGNRPRNFNDEKPKPVTPNGDNKIRVNKNWSTSKNKKEITFFVYEKETG
                     TKVGDFKMTADEQSKEYSEGLKQGTEYIVVEQPVDGHIPSYTVEQVDDKKHILSVTNN
                     PSDNPPPVVPEEPKVITYGKRFVKTNDVDLQQSEKLQGAKFVVKNTENKYLVLDSTAK
                     DVTAVWVDSKETATQFESKENGQFEVKGLKAGRYYLEEIKAPDGYALLSNAVEFEVNP
                     TSWGDSEVLSETNFLQIKNKKITIPQTGGIGTLVFTVIGLSTMVFAFIAIKKRRSEES
                     "
     gene            10591..11454
                     /locus_tag="VCA20_000497"
     CDS             10591..11454
                     /locus_tag="VCA20_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834355.1"
                     /translation="MRIIKQLVCCMLALVCSLSLPSLLVQAEETYSVQVGIPLPEHVD
                     AGKLDLSLEAWYLPEGDRETPEQLAEKLYDLSREELSQLYGQAQLSEKLTPQGVVGFS
                     GLKKGWYYIRQLGQATGIEVIPFVLQVDGQLQTVQAKVGLPGEKVGYRNFLKVSTSTA
                     PLAGAVFQVLEEVDGQLRQVMIDGKAYHLTSENGGHLKVGPLPYGSYYLKEVKAPAGY
                     ILAQDTIPFEITSDSHVSEIVKIKNKPVTPPGIDIPYTGNAVLVTVVSIGVLLFLLGY
                     RLVTYKKGKKT"
     gene            11638..11871
                     /locus_tag="VCA20_000498"
     CDS             11638..11871
                     /locus_tag="VCA20_000498"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834356.1"
                     /translation="MTKFRHSINPMIDIVAKKIFNDHEITIDFIETFLGFRPKSVQIL
                     NGTLADLKKEREGYFSTTVDVLARLDERDSGYH"
     gene            11879..12145
                     /locus_tag="VCA20_000499"
     CDS             11879..12145
                     /locus_tag="VCA20_000499"
                     /inference="COORDINATES: protein motif:HMM:NF024194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834357.1"
                     /translation="MVHQNGFLKRLWTYSCQHLVKDLPNVRDKVKRTHDMYDKISSVY
                     SIALVATQYFDDKQPIHSFVLTAEENGQVLEIPFGEQGGDEETI"
     gene            12129..12557
                     /locus_tag="VCA20_000500"
     CDS             12129..12557
                     /locus_tag="VCA20_000500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834358.1"
                     /translation="MKKPFEWVIIELNKLSEGKLAKNQRLWMEFFANREFSQQQTGAI
                     SRAEHLLDRNTWTEEERKMIDQLAYSAANHFGELETSFILGRKRGQEEGRLEGRAEGR
                     LEGQLKIARQMLSKHFADELIKELTGLSQEDLDGLKGEHR"
     gene            12647..12853
                     /locus_tag="VCA20_000501"
     CDS             12647..12853
                     /locus_tag="VCA20_000501"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834359.1"
                     /translation="MAQGLQKGIQKGIQKGRAEGMLDGQLKIARQMLVKNFTDELIKE
                     LTGLSQEDLDGLKTGGLDVTKADF"
     gene            13054..13884
                     /locus_tag="VCA20_000502"
     CDS             13054..13884
                     /locus_tag="VCA20_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEP1834360.1"
                     /translation="MRKGKKKNNLLFYLIFLIGLGILLYPHISNFYYRYEATHIVTDF
                     DQAKSGLASAEIKQRLDLAHAFNESLHNVLAEDPYSRSRHEAGRAEYARMLEINERIG
                     HVDIPKIEVDIPMYAGTSEEVLQKGIGHLEGTSLPVGGQDTHSVLTAHSGLPKARLFT
                     DLHRLKLGDEFYIHNIGDVLAYEVDQILVVEPSDFSQLLVVPNQDYVTLLTCTPYMVN
                     THRLLVRGHRIPYVEEEHRKATDQAEKRILIRYLLYALGMLVAIGLIFVLTKRKRRKK
                     "
     gene            13881..14738
                     /locus_tag="VCA20_000503"
     CDS             13881..14738
                     /locus_tag="VCA20_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEP1834361.1"
                     /translation="MMKNLLGSNRFLQKKLVLHLIFVLGLLITLYPLCSHLYYRYEAD
                     QEVQEFYQLAKDLPTQEVLNRLELARAYNKTLNPRKLQDPYSDEEKAGLAEYARMLEV
                     KEKIGYVEIPKLNEKIPVYAGTSEEVLQKGVGHLEGSSLPVGGANSHTVLTAHRGLPT
                     ASLFTNLDQLKLGDRFYIHNIAEILAYEVDQILVVEPSDFEPVLVVEEKDYATLLTCT
                     PYMVNSHRLLVRGHRIPYVPEVHEEEEPGFFVDRMLLSYLVAALLLILLLASYLLFQR
                     RKATRRRRS"
     gene            14735..15586
                     /locus_tag="VCA20_000504"
     CDS             14735..15586
                     /locus_tag="VCA20_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEP1834362.1"
                     /translation="MKLKIVGYLLMIVGLLLPLLLFSNMTVHEVREFFAYQSYKKLES
                     GFSKKEEEAVEHYQEVVRSGQLATVDPFAETKKAEQSVSDFGDTIIGYVSIPSLEIRQ
                     PIRVGASDSHLDQGVAVVSGTDLPFGGLDRRSVLAGHRSWYSDLRFFRLNEMREGDEI
                     FVEIGEKVVTYRVKNTEIIKATDWQKLLPIEKQDVLTLLTCDPLVPPFDYRLLVNAYR
                     HHDSSEQAVSTSEEKRQSSQAVLESYRKKGVSFIAYLTLFGWFLFVYLAYKLGRLVRE
                     CRVTKVR"
     gene            complement(15677..16504)
                     /gene="xth"
                     /locus_tag="VCA20_000505"
     CDS             complement(15677..16504)
                     /gene="xth"
                     /locus_tag="VCA20_000505"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEP1834363.1"
                     /translation="MKLISWNIDSLNAALTAESPRALLSRAVIDTLVTEDADIIAIQE
                     TKLSDKGPTKKHLEILESYFPGYANTWRSSVEPARKGYAGTLFLYKNHLTPTITFPEI
                     GAPTTMDSEGRIITLEFDDFYVTQVYTPNAGDGLKRLADRQIWDVQYAEYLSQLDSQK
                     PVLATGDYNVAHKEIDLANPTSNRQSPGFTDEEREGFTNLLAKGFTDTFRHLHGDVLN
                     AYTWWAQRSRTSKINNTGWRIDYWLVSDRIADKVSKSDMIDSGARQDHTPIVMEIEL"
     gene            16659..18320
                     /locus_tag="VCA20_000506"
     CDS             16659..18320
                     /locus_tag="VCA20_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834364.1"
                     /translation="MKRKLFIFAWLALVLEMLIYYWYQLPALNILSVDFWIFFLQSVG
                     LVWLILSMFSSKGAFQKVTKISGRQRQVDTYQIKTSQLPAYLKWLGRIWVLVVLGLLG
                     LVLINSRVFRAKDYAAVISVKDADFKADFPETDISKLALLDRASAEKIGDTYLGTIDK
                     VSQFGISDDYRQITIGQQPFRVSPLEYKNFWKWLSNHQDGIGYYVKVNQTTGKAELAK
                     LSKAMHFSDSEFLLNDTLRHLRLQYPTTIFGKPSFEVDDQGNPYYIATIYEPKFGLSS
                     NDPIGAIVLDAVTGESKKYSLEDIPEWVDRVYSADNVISRVDDHYTYQNGFWNTIFSQ
                     TGVKNTTDSYNYISIGSDIYLYTGITSATADSSNLGFILVNMRTREITNYKLASATET
                     AAQESAEGEVQEKGYKATAPSLVKLGDTAYYLVSLKDDAGLVKSYALVDAEDYQQVTV
                     NNDIALLISQVTGTDASSLSGLTVTENGEVGEQTEVISGKVEALASQIIGGATIYYIE
                     SEGKIYKVKATEDSTDRLPFIKVGDHFSGQLDKKNYLKNFKVNPE"
     gene            18480..19868
                     /locus_tag="VCA20_000507"
     CDS             18480..19868
                     /locus_tag="VCA20_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEP1834365.1"
                     /translation="MSLFRKKQAVGRHSQMRRHLGLVDLIFLGIGSMVGTGIFTVTGL
                     AAAQYAGPALIISIVIAAISVGLTALFYAEFASRIPTNGGAYGYLYSVFGEFPAWIAG
                     WLTIMEFLTAVSSVASGWGAYLKGLLANFGIAMPTALNGIFNPAAGTYVDLLPVLVLI
                     FVVGVVLLNSKAALRFNSALVVLKFSALALFILVGLFFIKPENWSNFSPFGFGAIYGG
                     QAGIMAGASLMFFAFLGFESISLAIDEVKKPEKNVPKGIVLSLSIVTILYIVVTLVLT
                     GMVHYTKLNVADAVAFALREVGLDWAASYISIVAILTLITVCISMTYALSRMVYSISR
                     DGLLPKSLSRLTQTSKVPKNATILVGIFAAICAGIFPLASIASFLNICTLAYLIMLAL
                     GIIRLRQVEGLPKDGQFKTPLVPLLPILSIIICLSFMFQYSLDTWLAFGVSLVIGILI
                     YFCYGYRHSEAK"
     gene            19991..20872
                     /locus_tag="VCA20_000508"
     CDS             19991..20872
                     /locus_tag="VCA20_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEP1834366.1"
                     /translation="MSNSDIRAKATVIREQTDGMITLFLAPVLISFLSGVAEFGLKQI
                     WGQTGTFSWGTTIVKDGVTWTHHFISVGPSIIFDFIAQCLIVTACFQLIRVVRKQRDK
                     VSFSECFSLLDGKNFLPILVTTFLKMLVIYIAGFPAVIGMALLSISFFSTVFIVGAPV
                     GYQPDFSGFFSSSYFQIGVILVVIGLVCSLLVDYGLSQVQFLLYDHLENNIYSSPFKL
                     FKQSWQLMKGNKWRRFMLDLSFIGWFIGILLTFGLLALYVYPYYWTCQALFYEDLIAK
                     NPLLLSKVDSVWFSELG"
     gene            complement(20971..21615)
                     /locus_tag="VCA20_000509"
     CDS             complement(20971..21615)
                     /locus_tag="VCA20_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEP1834367.1"
                     /translation="MKAAFISEKAKSRFNLNFILSPITALVLMAVAETVGYFAFMPLF
                     FIFIDNAFVTLSLELLAFAFISLAIILWARFVEKSPWLGLGIRKIGILVSSLFFTFLH
                     LGNDAISLIPLLDLPLFAILASLVMLKTGNIWVISGIHAAWNCFQGNVFAFPVSGTQA
                     GQAFIAVETSGPDWLSGGAFGVEGSVISLLIQAGMITWLVYELYFTSPSNQLEL"
     gene            21729..22340
                     /locus_tag="VCA20_000510"
     CDS             21729..22340
                     /locus_tag="VCA20_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3885 domain-containing protein"
                     /protein_id="HEP1834368.1"
                     /translation="MQLQDWLRDFQFGEHQLVGPFFYATPLALKFEVGPAEEAETLPK
                     KVYLDRAYARAVELLGQASLEYDYVILSLLRQEDRDIDTYLWHFTSKFNFNKCPEPEL
                     IEVEDWTGEVLVFERYLFPVADQDLKDLLKEIIRADHGGFNYLSSSVLFLSSRDKVIY
                     HCYDDRGVDVAVVDDDKRRQLFTDCQDLLFDYDMEEMVRRMES"
     gene            22398..25633
                     /locus_tag="VCA20_000511"
                     /pseudo
     CDS             22398..25633
                     /locus_tag="VCA20_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000401851.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
     gene            25635..26312
                     /locus_tag="VCA20_000512"
     CDS             25635..26312
                     /locus_tag="VCA20_000512"
                     /inference="COORDINATES: protein motif:HMM:NF025693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4391 domain-containing protein"
                     /protein_id="HEP1834369.1"
                     /translation="MPFLPSTQIPRPKVLYKPYQKGNKDFLDNFGLSTADKQLLRQQI
                     GQITATHQLDAKTLPIPAGKTIQQIIVLRIDLLSQQLDPHLLAELDTYLGFYTLFHLV
                     FPDGTSQYLIHFKEKLAQSREGRNFKIVRHFQTDKPLALTYQERDLDQFYENLVKQAG
                     EEELVGGGASVKDQIEQTERLAAMEKQAAQLKKKMFAEKVMRKQMELRKAYKALEEEI
                     LQLKNPQ"
BASE COUNT         8211 a         5067 c         6025 g         7425 t
ORIGIN      
        1 aaacatatat gaaaacaata tacacacata tatatgattg aaaccaagaa aatttcaaaa
       61 actttatcag aactctatgc aaattgctga aaaaaccgca ttcgtataca tatataacag
      121 tgaaatttgt ttgttttttc cgtaaactac ttttattttt ttgaaaaatt tagtataatt
      181 agctgaaatg acaacgtgat aaagaataac gatgcatata aagtagatga aatggggaaa
      241 agttatgaaa aaggtaagat actttcttag ttctttctta ttacttgttt tatgtctgga
      301 tattattcgg ctaccgataa caattttttc ggagtcagtt agtgctacaa cgccactaac
      361 cattcggact aagtatctag aacatctgaa ggaagatggt caaaagctgg ccattaagct
      421 agataccagt caaaagcagc tcgataggct agatgtgatt gtccagattg aagagggagt
      481 agacatcagc aaggggcttt ccttggtgct ccagaatgag ctggaactgg aaagtcagtt
      541 ggatgagcaa gggatgctca aggaaaaact tccatctggt tttgtccagg atgaagacta
      601 tcgtgaagaa gatgtgccca aggaatttcc tgaacaggaa gttgtcccaa ctgttgcaac
      661 acaggattct ctagaagctc ccttgcctgt atatggtcta ggctattact ccatcaaaat
      721 caagccagag cctgaacgcc atatagagtg gaaggtagat gaagagcagc ctgagcggag
      781 atacgaggaa attctgctgc gcttgaagat aaagaatccg ctacaaactg gtcaatttat
      841 ggccgttctg gtatcagatt ataagaccat cttaacagaa gactggaaaa catttgaaga
      901 attggtcaat aggaatagta tagagatggt gactgattcc gcagccttcg atacctcaca
      961 accagtctca gaaaaatcgg tttctgatac cgagcagcca attccagaaa cttctgaatc
     1021 tatttctgac aacacgcagt ctaatccagt acctgaaggc gaagaggtga tacaagaaga
     1081 acaagcttta gccattcaaa cagctaatct acgcttggta ttacgagaac atgaagaagg
     1141 tgtcttgcaa cctagcaagc ccatcgcagg tgcggagttt tctatgatag ctgtaggaaa
     1201 tcctgaaaac gctcacaaag gtcgtacaga tgaacaagga gtcttggaat ggcgagattt
     1261 acccgtaggt gagtaccaaa tcagtcaatt gtcaacagct caaggttacg aagtagagaa
     1321 acagttgcca gtcattcagt tgcaagaaag tggagtcgaa aaaataatcc atgtcaccaa
     1381 taccaaaaag aatgagttag aaggacagat tggtagtatg ttgcgctctg ctcggactag
     1441 gagagctgct acagaaacga atacggatag aacaacaacg attatcaata atccgaatgg
     1501 aacaaccact accattacga aagaagagga aatcaccaag gatatttttg acccgattcg
     1561 gattgagatt ccgggagtaa cgcgtcattt tagacaggga ggagatttac gcaagaaaga
     1621 aattcaggtt acctctactg gtgaaaagaa gattatttgg gaatatatta caaccgttac
     1681 tggagcaagt gatagatata tcaaacaaat cgcaaatact tttatggtac cagttgactc
     1741 aggacttgga acacctaaga ttacatctat tagctacaac caaggacaaa cgattgaaaa
     1801 tcctgagacg agtcggtata gtgattctgg tagatttaat tctgttacaa atcagttgcc
     1861 gattcaagat ggcacatatg tgtacaccat tgaaacaccg attcttcttc caagtgagaa
     1921 ctacacactg gattattatt cggatgttaa ggtgcaagcg ccgatacgtt cccgtttgac
     1981 acaaaatgga caaacgattg tattaacaag ggaggaggag cggaacatta aaagtgcagg
     2041 tagtttgacc ttgccaagtg acaacaccac ctcaccctat gatacaccta aattatcaga
     2101 taccattcga gtgaatggtc gctatcgcga gtctgataat aaggtgattg agtggtatac
     2161 ctctcgtctc aatacgacag atagtgtaca aacgtataca tttgacgttg ctgaagataa
     2221 gaatacacaa gcggttcatt caaagattgt ctatgtttat gaacctaccg atgggggaaa
     2281 ttataagcta aagagtcgtg cacaagttga taaaacgcaa agtcaaatta ccgtaaatga
     2341 tgttccagta ggaggtattg cagttgtcga aacaatcacc aacgtgacaa atgaaaaagc
     2401 caatcatacg attacgggtg catcgctaga agctttaaaa ggtgatatta caatacagaa
     2461 aaaatgggca actggtacgg agaagactgc tgttacctat actattaacg gaggaagtta
     2521 taataaccat aaagtaacct tgcaagcaaa tcagtcacag gtaataattc caaatgtcga
     2581 aaagttttct ggaacaggca gtactgcctc aaaaacacga atatactatg aagtgaccga
     2641 aaatgttcca gatgactatg cgctttctac tgcacagatg gattggaaaa acttgcagta
     2701 tgtctttacg aataaaaagg atactagcca acctatcaca cctacgccac caggttcttg
     2761 tgaatcatac ggtgtcacac atgttgaacc agtcaatatc aactggttga cttggcaacg
     2821 aggtagtaat agatggggag gattccacgg gtcgttaaaa ttctacttca aattaccagc
     2881 ttttgctaaa gaaggtgata gctttacaat tgaattgcca agagaattaa aactcgaaca
     2941 cttagcaaat ccatctgtcg aatggacacc tgtttataaa tcaggtacaa gtgaaatcct
     3001 tgctcggacc taccatgtag aagatagaaa attaaaattt gttctgacat ctgctgctta
     3061 ctctgtgacc gaatacagtg ggtggtttga aattggtagt gatcagagtg caaacctagt
     3121 tcagataaat aacgacaaga atgtagctgg aaaaaaatat ctgactggtg tgcgtccaga
     3181 agtacctgtc tggtattctt ttgaccctga aaatccaatc aatacacagt ctaacattgt
     3241 gaataaaaag ttgaagtatc agactagcta caaaggtgga cattttgact gtagtcatac
     3301 ggtagttggt gacgggacac tcaatgcgac agagaatggt agcggtggtc tacaaggaag
     3361 tttacgtcaa ttttcttccg tccacaaaca agcgatcaat cagacagctg attctgtgac
     3421 ctgggagttg gtttataacg gaggtaggaa cgatatttcc ggcagtattt tagcaatcag
     3481 acggaagttt tgggatcgta tacatgtgga tacccccctg tacgatggca atacccatac
     3541 aagtattctt aaagatttag aaatttttgt atcagatggg actcctgcag tttcttatgt
     3601 tgagtcagaa atggtaaaac ttaaatatag aacaacctca aatagtggtg agaatacaac
     3661 atatcattat ggaattgatg gtaccgatat gggactggct gtcctgttga aacgtgagaa
     3721 ttggggagat aatagttttg gttttcagtc aaactatgca cttgagtttt actatgaagg
     3781 aagagattgg ggaaggaagg caatcattgt tcgtataaaa caacgaaaaa atggaaatac
     3841 tacactgaga aatgcagtca atgtaaattg gaatagccta gtaatcagtg gaaagatgca
     3901 tacaaataac tattttgttg ctggagatac ggttgcgagt ggtggtgcct atccgacaga
     3961 ttggtatggc ttccggctta agaaagtaga tcagcaaaat cgtccgttag aaggcgcggt
     4021 ctttactctc tacgactcac aacagcgccc ttatcaaacc attcgttcaa atgaacaagg
     4081 tgttcttgaa tttacaaata tcttaggagg cgagtacact ttcaaagaag ttgaagcacc
     4141 agcgggttat acgctagata cgacagtcca tagattgatt attagaaatt cagaaaatat
     4201 aacgattgat ggacaatcct atactgcaca agcaccgttt gaaattataa acaagaagga
     4261 tgcgaaactc agcctcaaac tcaagaaaaa agatttggca aatactccat tgaaaggtgc
     4321 tgttttccgc ctgagaaaaa cagatggtgg tcagccatat gatgagacgc tcggagctac
     4381 atttgaatca gacgagttcc tctttaatga gttgacagta gggaactaca cgctggagga
     4441 agtaagagca cccacaggct atcgtggaat ggggcagatc aacttaacgg tttatgaaga
     4501 aaatggaacg cttaaactgc gtaaacaggc agacacggct aacagccaga ccagtactcc
     4561 tcaactgacc aatggtgtgg tgactctgga tatctataat caaccgcttg aactaacgtt
     4621 tacgaagcaa aatgctcagg gagaaccatt ggaaaatgca atctttgaat tgcgtcagat
     4681 acactcaact ggctttacag ttgtacgaac aaacctcttc tctaaagcca atggacaatt
     4741 tgtcctgaaa ggtctcttgc caaataccac ctatgaactg tgggaaactc gtgcaccaga
     4801 tggctatgca aaaccaactc aagcagtggt tcagtttact gtaaacgaag atggaacagc
     4861 ccactttact cgtggcagtt cgactattgt taactctcca ccgaaattta aggttaagat
     4921 tgtaaaaatc gatgcggaga caggacaacc gattacggaa ggcgtcaacg ataccgcccc
     4981 acgtttcacg gtgacaaatg ctacaggaga cacgcttaat ggtcaaattg ccaatctgca
     5041 aaatggtatg tatacattca caaatggaag cggtactaat tctggatttg aagcaggaac
     5101 ctactatctt aaagaaatcc gagcaccgca agggtataca cggctgacaa acctaatacc
     5161 atttacaatc cactcagatg gacgtgtaac agtgagtgaa gagtttgcgg atggaatagc
     5221 caccagcgca acatctccag atacagtaca aatcaaagtc aaaaacgacc gattaaaaac
     5281 cttgcgtatt tccaaacgca tcaagggatt ggaaaacttg ccgagtctta tcacaggaca
     5341 gatgaccttt acattgacca aggacaatga tgcaaccgca gtatcagctg ttcagacaca
     5401 tgctgcgaca gaagacttcg tatttaataa cctgacctac ggttcttaca cccttgttga
     5461 aacagcgcca ccagtgggtt acatggtgga accgattgtt tataaagttg cggtcagtcg
     5521 agcaggagta tccctttatc gaacaagtga ccaagtgaca ccgagtcgag tcgaaggggc
     5581 gagcctaatc acatctcctg atttgagtaa tattcagagt ggtcctgaga gcaatgccat
     5641 tgatggaagt gaaacaactt ctgtgaccta tcataatttc aatggtcagg gaaatactat
     5701 cccagtcggt gcctatctag gcgttgacct ccaaagcgtc aaacgagttc gaaatatttt
     5761 ctacttacaa ggaaatcaga ataatgtaac ggataggctg aatcgctaca cagtagaata
     5821 ctcaatagat ggtgagacct actacccaat cgcttcatca gcaacggctg agcgctttga
     5881 acagtctacc gaccttctcg ctcggtatat ccgtgtgaga aatgaagagc aagtcgtcaa
     5941 caaatggtat gcggttcgtg agctacgtat ctcagcccaa gaagcggagt ccgtaagtct
     6001 tcaaggtggg caggcattcc caataggaaa tatccaacat cctgagattc aaatcgagaa
     6061 gcaggatatg aataaccgta ttatccaaca agcagtgaca tttaagctct ataaagttcc
     6121 agatgataca acgtcacaaa cggtgacagg cactctgaca gatcaaaatc ttgtccaaac
     6181 ctttaccttg acaaatgggc aagttcgtca gaccttagaa gccaaggtgc aaggtcgcta
     6241 tgcccttgtc gaaggcacac cgccaactgg ttatcgtggt ttagctagtc caatcttact
     6301 agacttgcag acactacagc aaccccatag cggaagccag atgaagacaa cacgattcag
     6361 cctcgttgga acaaatgata aggtgaggat tgataccaac acagccaatg tgctgaagtt
     6421 catcgtaaaa aatgaacctc tgacctatca tctcaagata ctcaaacgcg atgtcaataa
     6481 tgcggcgaca ggtctcgatg cacacttcga gctgtatgat gcaacgggta cgactgttat
     6541 cgaacgtgga aataccagtc gggacggatt tagcttgacg ttcccaaacc tgagtccgaa
     6601 tagctatgtc ctgaaagaga cagaggctcc tgccaattac aatccaatag cgccgattca
     6661 tttgaccatt gaccaaacag ggcagctaac tatccagtca ggaccaagtg acttaatcgc
     6721 tattcaatca gcagatgaca gtcatcttat ccaactaacc atcaagaata gaccgtttac
     6781 gccaatcagt attcagaagg tggacaaacg tagcaacgag acaaccttga caggtgtgcg
     6841 gttgcggatt caggctaaga atggcgctca gatgccacaa tttagtgatc gaagctggca
     6901 tgaaacccat tatcaaggtc gagttattga cagcgagaaa gctcttgagt ggaatagctc
     6961 caatgcaggc aatgctgtct tcaagctacc acaaggaacc tataccatca gcgaactctc
     7021 tgcaccagcc ggttacgagc agctacagcc tttcgatatc actgtggcag aagatggtag
     7081 tgtccagcta ggtcagggaa caaatactga gcaggttagt cagggacaaa aagacggacg
     7141 gatcaatatc aaacttacca acgtctttaa gcctaagata aagattacca aagtcgacag
     7201 ccgtgacgcc aaccgcaagc tagcaggtgc acgctttaaa ctatttggac cggatgaaaa
     7261 tacgcagata ggtcaggaag tgacaaccgg cagcaatggt gaaatcacgc tgccaggctt
     7321 ggaaccaggg acctactatt tacaagaaag ccagccaccg acaggttatc aggctaacac
     7381 tcgcaaatac cagttgacca taggcaatga tggtgccacc cagttggtca atggagatgc
     7441 taagattaaa attggcaata gccaagatca aggggcagat aaactcatcc caattaccgt
     7501 aaaaaatgat cggacaccgt ttacagtgaa aatccgtaag cgggattaca atgatgtcaa
     7561 tagaggttta aacgctcgtt tccaactcta tcgaaaaaat ggcaatcaag aagaactact
     7621 ttctggatta gttggagata ccatagtgaa taatcatgag ttggttcttc caaatatctt
     7681 gcctggaacc tatatcttga aagagataag ggtgccatct ggaaattatg aagcgattca
     7741 accgattcaa ttcaccatca cggaagaagg tcgcctgcag attgatgtca atgatcgtga
     7801 ttttgcctcg gtagaaaatg gtggaagcag tcaggaactt gtcttgctga ttaaaaacat
     7861 caagaaattc cgtctgcgca tcaaaaagca agatgcgacc aatgaccaaa ccttgcttga
     7921 tggtgcgaga tttactcttt atcgtgccca aggcaataca ggtggtaagc tagaacagct
     7981 tgcgactggt gtaactgcca atggactagc cgacattgat gttggtccag gctactttat
     8041 cctacaagaa atccaagcac caacgggcta tgtcctaaat ggtaaggaat acattttcca
     8101 aatcaatcac gacggaacag ttgctttaca taacagtgat gatatgacct ctctacaggg
     8161 ggcagacaat aaccgtgtgg ttgtctttac catgaagaac agaagaaaaa caggacagtt
     8221 taagctagcc aaacgtgctt atagcgatag cgatcgtcgt ctcgcagcgg tgtttgaatt
     8281 gaaagaagag aatggtgcga ctcctgttgc caccaagacg acagcgaccg atggagaaga
     8341 aattgtcttt gacaacctcc gtatcggtca gatttaccaa ctgaaagaaa cccaagcccc
     8401 tgaaggctat cagctcaata cgaaagttta ccgacttcgt cttaccgata gtggtcaagt
     8461 ggaactccta gacggagatg acctcttgtc cctagatgat agcaataggc agttgcttac
     8521 tgctaaaaac ctgaaaaaag gggaatatcc taagacagga ggttttggag ttctaccata
     8581 tatcacattg ggaggaggga tgatgttact ggctcttgcc gtcgagctag gaaaagaaaa
     8641 atgttggaaa cgtatacgta aatagctgaa taaaaagaaa aggagaaaat aatgaagaag
     8701 ttaacaaaat tattctccct cttgaccgtt cttctgacga tgttcggtcc attgggaaat
     8761 cttcgacatt tagtacatgc gaatgatgcg caaccgcata gaacagctgt tgttgttcat
     8821 aaagttttga tgacaccaga acaattagaa aattttgatc atgatcaaaa acaagtaacc
     8881 aataaatacg ttggtaatca gattcaaaat tttggagaat attttggtga aggttcaaaa
     8941 gaaattgaag gtgtaaattt caaagtttgg actaaagttg atagagcaga agatggtgtg
     9001 actaaaactg gtatcgaact tggaattgct gaagataata ataattataa attaaaccaa
     9061 agctatccag atggagtggt aacattagct gatactcaag gggcaacttt tgaattagaa
     9121 gatggcactt atatctttgt ggaagataag gaaaactcac catattacaa taaagatgga
     9181 gtgaccactg caaaagatgc aagtaaagaa ttgactcaat ctaaggctgt tccatttaaa
     9241 cttgaattgc cagtaacaaa accagatggt caagggtatt ttgacttgca aacaaaactt
     9301 cacgtttatc caaaaaatac agaagataag ccagatgtga aaaagacatt tggtgatgga
     9361 aatactggag tgaaagaata tgatgtaggt acaaaaattc catacaaaat cacaacaaga
     9421 attccaaaag gttctaccta tagaacgctt atttgggatg acttgatggt caatggattg
     9481 gattttgatg taactactgg agtacaaatt gtatcgaaag aagttcaaga ttttaataat
     9541 agacattatt cagttgaaaa agacattcgt ggttttgttc ttaaagtcaa ccaagaaggt
     9601 ttgaacttga ttgaagaagc tgcaaagaca caagatgtta atttcactct tacctacaat
     9661 ggtgtattga atacttccgc acaaatcaat acacaaatcc cgaacaaggt aacattccat
     9721 tttggtaaca gacctcgtaa ctttaatgac gaaaaaccaa aaccagttac accaaatggt
     9781 gataacaaaa ttagagtaaa taaaaactgg tcgacaagta agaacaaaaa agaaattaca
     9841 ttctttgtat atgaaaaaga aactggtaca aaagttggcg actttaaaat gactgcagat
     9901 gaacaatcga aagaatattc agaaggtctt aaacaaggaa ctgagtacat tgtagtcgaa
     9961 caacctgttg atggtcatat tccatcttac actgttgaac aagttgatga taagaaacat
    10021 attctttctg tgaccaacaa cccatcagat aacccaccac ctgttgtccc agaagaacct
    10081 aaagtaatta cttatggcaa gcgttttgtg aaaacaaatg atgttgattt gcaacaatct
    10141 gaaaaacttc aaggtgcaaa atttgttgtt aaaaacacag aaaataaata cttggtttta
    10201 gattctacgg ctaaagatgt gacagctgtt tgggttgatt cgaaagaaac tgcaactcaa
    10261 tttgaatcaa aagaaaacgg acaattcgaa gtcaaaggtt tgaaagctgg aagatattac
    10321 ttagaagaaa ttaaagcgcc ggatggctat gctttgttaa gtaatgcagt agaatttgaa
    10381 gttaatccta caagttgggg agattcagag gtactaagcg aaactaactt ccttcaaatc
    10441 aaaaacaaaa agattaccat tccacaaact ggtggtattg gtacccttgt cttcacagtt
    10501 attggtttga gtacaatggt atttgcattc atcgctataa aaaaacgccg atctgaagaa
    10561 tcctaggata gagtagaaaa ggagggactg atgaggatta ttaaacagct ggtctgttgc
    10621 atgcttgcac tggtctgttc gttgtctctg ccctctcttt tggttcaagc agaggagacc
    10681 tattctgttc aggtcggcat tcccctgcca gagcatgtgg atgcgggcaa gctagatttg
    10741 agtcttgaag cctggtatct gccggagggg gatcgagaga ctcccgagca actggcagag
    10801 aaactctatg acctgagtcg agaggagcta agtcagctgt atggacaagc ccagctttct
    10861 gaaaagctga cacctcaagg agttgtagga ttttcaggct tgaaaaaggg ctggtattat
    10921 attcgccagc tgggtcaagc tactggtatt gaggttattc cctttgttct acaggtagac
    10981 ggacaattgc agactgtaca ggcaaaggtt ggactaccgg gggaaaaagt gggttatagg
    11041 aattttctca aggtttctac cagtacagca cccttggcag gagcggtttt ccaagtcttg
    11101 gaagaggtgg acggtcaact gaggcaagtg atgattgacg gcaaagccta tcatctgacc
    11161 tcggaaaatg gtggtcacct caaagtagga ccgctcccct atggtagtta ctatctgaaa
    11221 gaggtcaagg ctcctgcagg ctatattcta gcccaagata cgattccttt tgaaattact
    11281 tcggattcac atgtgtctga aattgttaaa atcaagaata aacccgtcac acctcccggt
    11341 attgacattc cctatacagg aaatgcagta ctggtgacgg tggtgtctat cggagtcttg
    11401 cttttcctct tgggctaccg cttggtcacc tataagaaag ggaaaaagac ttagtctgcc
    11461 tttggtcata ttgttgacag aatacacctg ctggaatcaa gtgtcagcag gtgtattttt
    11521 ccttgaaaac attaagtgtt tctggaaaaa tatccttcaa gatgctataa tattactagg
    11581 aacttcgaat aaatagatag agagtcatgc tcaattctat ttatcggagg aacacaattg
    11641 acaaaatttc gacatagcat caatcccatg attgatatcg tggcaaaaaa gatatttaat
    11701 gaccacgaga ttaccattga tttcattgag acctttctgg gctttcgtcc caagtccgtc
    11761 cagattttaa atggtaccct tgccgatttg aagaaagaac gtgagggcta ttttagcacc
    11821 acggtggatg ttttagcgag attggacgaa cgggactcag gttatcattg aaattcaggt
    11881 ggtccatcaa aatggctttc tcaaacggct ctggacatac tcttgtcagc acttggtcaa
    11941 ggacttgccc aatgttcgtg ataaggtcaa acgaacccat gacatgtatg ataagatttc
    12001 gtctgtttat tccatagcct tggtcgccac ccagtatttc gacgataagc agcccatcca
    12061 ttcctttgtc ttgacagctg aggaaaatgg acaggtgttg gaaattccgt ttggagagca
    12121 agggggagat gaagaaacca tttgaatggg ttattattga attgaacaag ctgagtgagg
    12181 ggaaattggc taagaaccaa cgcttatgga tggaattttt tgccaatcgt gaatttagcc
    12241 agcaacaaac aggcgctatc agcagggccg aacacctgct ggatagaaat acatggacag
    12301 aggaggaaag gaagatgatt gatcaactag cctacagtgc ggccaatcat tttggtgaat
    12361 tggagacttc tttcatacta ggtagaaagc gtggtcagga agaaggtcgg ttagaaggac
    12421 gggcagaagg ccgattagaa ggtcagctca agattgctcg gcagatgtta tcgaagcatt
    12481 ttgcggatga gctgataaag gaactgacag gactaagcca ggaagatttg gatggtttga
    12541 aaggtgaaca tagatgattg attaactagc ctatagtgcc gccaatcatt ttggcgaatt
    12601 ggagacttct ttcatactag gtagaaggcg tggtcaggaa gaggggatgg ctcaaggact
    12661 tcaaaaaggt attcaaaagg gtattcaaaa aggtcgagca gaaggtatgc tggatggcca
    12721 gctcaagatt gctcgtcaga tgttggtgaa gaatttcacg gatgagctga taaaggaact
    12781 gacaggacta agtcaggaag atttggatgg tttgaagacc ggaggattgg atgtgacaaa
    12841 agctgatttt tagaattgaa tgttgagcat ccaaagagca actgagtgag acggacgaat
    12901 taaagaattt tctatggtca tctgtgttgt ttcaaacgat aggaatcagg aattgcgttg
    12961 ttttagttgc aatttttgtt agcttggaat gatatagtgc gtaactattt tttcattaat
    13021 gtatcacgtt gtagcacatg gaggtgatgt tgaatgagaa aagggaaaaa gaagaataat
    13081 ctccttttct acttaatttt tttaatcgga ttgggaattt tactctatcc ccatatttca
    13141 aatttctact atcgttatga ggcgacgcac attgtaacgg attttgacca agctaagtca
    13201 ggcttagcct cagctgaaat caagcaacgt ttggatttgg ctcatgcctt taacgaaagt
    13261 ttacacaatg ttcttgcgga agatccctat agtcgttctc gtcacgaggc tggtcgggcg
    13321 gaatatgcac gcatgttgga aatcaatgag cggataggac atgtcgatat tcctaagatt
    13381 gaagtggata ttcccatgta tgctgggact tcagaggagg tcttgcagaa agggattggg
    13441 catttagagg ggacttcctt acctgttggc ggtcaggaca cccatagtgt attgacggct
    13501 cattctggtc ttcccaaggc acgcttgttt accgatttgc atcgtttaaa gctaggagat
    13561 gaattttata ttcacaatat cggagacgta ttggcttatg aagtggatca aatcttggta
    13621 gttgagccga gtgatttttc tcaattattg gtcgttccaa accaagatta tgtaacctta
    13681 ttgacttgta cgccttatat ggtgaataca catcgacttt tggtgcgagg acatcgcatt
    13741 ccttatgtgg aagaagaaca tcgcaaggca acggatcagg cagaaaaacg gattttgata
    13801 cgctatctct tatatgcctt aggcatgctt gttgccattg gtttgatttt tgtcttgact
    13861 aaaaggaaga ggaggaagaa atgatgaaga acctgctggg atctaatcgc tttcttcaaa
    13921 agaagttggt cctgcatttg atttttgtgt tgggactgct cataacgctc tatcctttat
    13981 gttctcatct atactatcgc tacgaggcag atcaggaagt gcaggagttt tatcaactag
    14041 ccaaagacct gccgactcaa gaggtgttaa atcgtctcga attggctagg gcctacaata
    14101 aaactttaaa tccaagaaaa ttgcaggatc cctattcaga tgaggaaaag gctggtctag
    14161 cagagtatgc tcggatgttg gaggtcaagg agaaaatcgg ctatgtcgag attccgaaac
    14221 taaatgaaaa aatccctgtc tatgcaggaa catccgagga agtcttacaa aagggagttg
    14281 gtcacttgga aggttcttcc ttgcctgtcg gtggtgccaa tagccatacg gtcttgacag
    14341 ctcatcgagg tcttcccacg gcctctcttt ttaccaattt ggatcaactc aagctgggag
    14401 atcgatttta tattcataat attgctgaga ttttggccta tgaagtggat cagattttag
    14461 ttgttgaacc tagtgatttt gaacctgtgt tggtagtaga agagaaggat tatgccactc
    14521 ttttgacctg tactccttat atggtcaata gccatcgctt gttggtcagg gggcatcgga
    14581 ttccctatgt tccagaggtg catgaggaag aagaaccagg tttctttgtc gataggatgc
    14641 ttttatccta tctggtagct gctcttctcc ttattctctt attggcaagt tacctccttt
    14701 ttcaaagaag gaaagcgact aggaggagac ggtcatgaag ttgaaaattg ttggctatct
    14761 attgatgata gtgggattat tgttgccctt attattgttc agcaatatga cagttcatga
    14821 ggtgagggaa ttttttgcct accagagtta taaaaagttg gagtcaggct tttcaaagaa
    14881 agaggaagag gccgtcgaac actatcagga agtagtgcgg agtggtcaac tggcaacagt
    14941 tgatcccttt gcagaaacta aaaaagcaga gcagtccgta tccgattttg gagataccat
    15001 catcggatat gtaagtattc ctagtcttga aatccgccaa cctattcggg tgggagcaag
    15061 tgattcccat cttgatcagg gtgtagcggt ggtgtcagga actgacttgc ctttcggagg
    15121 tctggatcgt cgtagtgtgc tggctggtca cagaagttgg tatagtgatt tgcgcttttt
    15181 ccgattaaat gaaatgcgtg agggcgatga gatttttgtt gaaattggtg aaaaagtagt
    15241 cacctatcgg gtcaaaaata cagaaatcat caaggcaacc gattggcaga aattgcttcc
    15301 gattgaaaaa caggatgtct tgaccctgtt gacctgtgat ccactggttc ctccttttga
    15361 ttaccgtctt ttggtcaatg cctatcgcca tcatgatagt agcgagcagg cagtatcaac
    15421 ttcagaagaa aaaagacagt ctagccaagc agtgctggaa tcttatcgta agaaaggtgt
    15481 aagcttcatc gcctatctga ccctttttgg ctggttcttg tttgtctatc ttgcctacaa
    15541 gttgggcaga cttgtgagag aatgtcgagt tactaaagta cgataggtat agtgtaacag
    15601 gaataaaagg aaaaagactt agaaccaccg tttctgatgg ctgttctaag tctttttgtt
    15661 tggagaactg tattatttat agctcaatct ccatcacaat tggtgtatgg tcctgacgag
    15721 caccggagtc aatcatatct gacttactta ctttgtcagc gatgcggtcg ctgacaagcc
    15781 agtaatcaat tctccagcct gtgttgttga ttttactggt gcggctacgt tgtgcccacc
    15841 aggtataagc gttgagaaca tcaccgtgca agtggcggaa ggtatctgta aaacctttag
    15901 ctagaagatt tgtaaaacct tcacgttctt cgtctgtaaa tcctggtgac tggcggttgc
    15961 tggttgggtt ggccaggtca atttccttgt gagcaacgtt gtagtcacct gtggctaaga
    16021 caggcttttg gctgtcaagc tgtgataggt attcagcata ctgaacgtcc cagatttggc
    16081 ggtcagcaag ccgtttcaag ccatcaccag catttggcgt ataaacttgt gtgacataga
    16141 agtcatcaaa ttctagcgtg atgatacgcc cttcagaatc catggttgtt ggagcaccaa
    16201 tctcagggaa ggttatggtt ggagttagat ggtttttgta gaggaagagt gtaccagcat
    16261 agcctttgcg agctggttct acagatgaac gccaggtatt ggcatatcca gggaagtagc
    16321 tttccaaaat ttctagatgc tttttagtcg gtcccttatc tgacaatttt gtttcctgaa
    16381 tggcgataat atcagcgtct tctgtcacaa gtgtatcgat gactgcgcgt gataataggg
    16441 cgcgaggaga ctctgcagtc aatgcagcat taagagaatc aatgttccaa gaaatgagtt
    16501 tcataatttt ccttttctag tctgttactg ttttacatta taccaaaaat aaaagttgca
    16561 ggcgaatatg gaaacttgaa aaaattttat aattcttgta ttataacatt aaaattgtga
    16621 tacaatagag ccaataaatc tgtatcaagg aggttattat gaaaagaaaa ttatttattt
    16681 ttgcctggtt agccctagtt ttggaaatgc taatctacta ctggtaccag ttgcctgctt
    16741 taaatatttt gagtgtagac ttctggatat ttttccttca atctgtcgga ttggtttggc
    16801 tcatactttc catgttcagc tccaaaggtg cttttcaaaa ggtgaccaag atttccggtc
    16861 gccagagaca agtagatacc tatcagatta aaacctcgca actgccagct tacttaaaat
    16921 ggttgggacg aatttgggta ttggttgtcc tgggtttgtt ggggctggtg cttatcaact
    16981 cacgtgtatt tcgtgctaag gattacgctg ctgtcataag cgtcaaggat gcggatttta
    17041 aagcagattt tcctgaaaca gacatatcca aattggccct cctagatcgc gcctcagccg
    17101 aaaaaatcgg tgatacttat ctaggtacca ttgacaaggt ctcccagttt ggcatttcgg
    17161 atgattatcg ccagattact atcggtcagc aaccgtttcg tgtatcgcct ctggagtata
    17221 agaatttttg gaaatggctg agcaaccatc aagatgggat tggttactac gtgaaggtta
    17281 atcagacaac agggaaggca gaacttgcca agttgagcaa agccatgcac ttttcagatt
    17341 ccgaattttt gctgaatgat accttacgtc atcttcgcct gcaatacccg acaactattt
    17401 ttgggaaacc atccttcgaa gtggatgacc aaggaaatcc atattatatt gcaacgattt
    17461 acgagccaaa atttggactt tcatcaaatg atccgattgg tgctattgta ttggatgcag
    17521 taacagggga aagtaagaag tatagtctgg aagatattcc agagtgggta gatcgtgttt
    17581 attcagcaga caatgttatc agccgtgtag atgaccacta tacttatcaa aatggtttct
    17641 ggaataccat tttcagtcaa actggtgtga aaaatacaac agacagctat aattatattt
    17701 ctatcggctc agatatttat ctctacacag gtatcacctc agcaactgct gattcatcca
    17761 accttggatt tattcttgtc aatatgagaa ctcgtgaaat taccaactac aaattggctt
    17821 cggcgactga aacagcagcg caagagtccg cagaaggtga agtgcaggaa aaaggctata
    17881 aagccactgc tccaagccta gttaagttgg gagatacagc ctactattta gtatccttga
    17941 aagatgatgc cggcttggtt aaatcatatg ctcttgttga tgccgaagat tatcaacagg
    18001 ttactgttaa caacgatatt gcgctcttaa tttctcaagt cacaggaacg gatgcttcta
    18061 gcctttcagg attgacggtg actgaaaatg gagaagttgg tgaacagact gaggttatca
    18121 gtggaaaagt agaagcttta gcgagtcaaa taattggcgg tgcgacaatc tactatattg
    18181 agtctgaggg caagatttat aaagtcaaag caacggaaga tagtacagat cgattaccat
    18241 ttatcaaagt aggagatcac tttagcggtc agttagataa gaaaaattat cttaaaaact
    18301 ttaaggttaa tccagaataa tcataagaaa gagtgaggta gaaaagcctt gctcttttgc
    18361 ttgtcgtcac ccatgcgaaa tatttcaact tatggtataa tggggcgttg tggttctttg
    18421 acaggaatcc gtagattatt ctgtcgagta tccatattct tattctgaag gagtattttg
    18481 tgagtttatt tagaaaaaaa caagcagtcg gtcgtcatag tcaaatgcgt cggcatttag
    18541 gcttagttga tttgatattt ttaggtatcg gttcgatggt tggaacaggt atctttacgg
    18601 taactggttt ggcagcagcc cagtatgcag ggccagctct gatcatctca attgttattg
    18661 cagcaatttc agtcggacta acagccttgt tttacgcaga atttgcctcg cgtataccga
    18721 ctaacggtgg tgcctatggt tatctctact cggtttttgg tgaatttcct gcctggattg
    18781 ctggctggct gacgatcatg gaattcttga ctgctgtatc gagtgttgca tctggttggg
    18841 gagcttattt gaaaggactc ctggctaatt ttggtatcgc tatgccgaca gctctgaatg
    18901 gaatttttaa ccctgctgca ggtacctatg tagacctctt accagttctc gtcttgattt
    18961 ttgtggtagg agtggtcttg cttaattcta aggcagctct tcgtttcaac tcagccttgg
    19021 tagttttgaa attttcagcg ttggctttat ttattttagt agggttattc tttatcaaac
    19081 cagaaaactg gtcgaacttc tctccttttg gttttggtgc catttatggt ggacaggcag
    19141 gaattatggc aggagcgtct ctcatgttct ttgccttcct gggatttgag tctatttcat
    19201 tagcgattga tgaggtgaaa aaacctgaga agaatgtacc aaagggaatc gtgttgtctc
    19261 tatccattgt aacaattctt tatattgtag tgacattagt cttgactggt atggttcact
    19321 atactaagtt gaatgtggca gatgcggtag cctttgcact tcgagaagtt ggattggatt
    19381 gggcagcgag ttatatttct attgtagcaa tcctaacatt gattacagta tgtatttcga
    19441 tgacctacgc cttgtcgaga atggtctatt ccatcagtcg tgatggtttg ctaccgaaat
    19501 cattgagccg attgacacaa acaagcaagg ttcctaaaaa tgctaccatt cttgtgggga
    19561 tttttgcagc catttgtgca ggtattttcc cactagcaag tattgcttcc ttcctcaata
    19621 tctgtacctt agcttatttg attatgctgg cgttgggtat tattcgtctg cgtcaagtcg
    19681 aaggtttgcc aaaagatgga caatttaaaa cacctcttgt tcccttgtta cctatcctct
    19741 cgattatcat ttgtctttca tttatgttcc agtatagttt agatacttgg ttagcattcg
    19801 gagtatcctt ggttattgga attttgattt acttctgtta tggctatcgt cattcggaag
    19861 caaaataata gatttccctt ctttatttga agggattttt tgtacaattt ttttatagaa
    19921 ataatggaaa acttaaaaat aaaatagtat aatatatata acgatacaaa tatatgaaag
    19981 gagaaacgca atgtctaata gcgatattcg agctaaagca acggttattc gtgaacaaac
    20041 agatgggatg ataaccttgt ttttggcacc tgttttgatt tcttttctgt caggagtggc
    20101 agaatttggc ttgaaacaaa tctgggggca aactggtact ttctcctggg gcacaactat
    20161 tgtgaaagat ggtgttactt ggactcatca ttttatttct gttggcccgt caatcatatt
    20221 tgattttatt gcacaatgtt tgattgtgac ggcgtgtttt caactcataa gagtcgtaag
    20281 aaagcagcga gacaaggtca gcttttcaga gtgttttagt ctactggatg ggaagaattt
    20341 tttacctatt ctggtcacga cctttttgaa aatgctcgtt atttatatag ctggtttccc
    20401 agctgtcatt gggatggctt tacttagtat ttcatttttc agtacagttt tcattgttgg
    20461 ggctccggtt ggctatcagc ctgatttctc aggatttttt agctcctcct attttcaaat
    20521 aggagtaatc ttagtcgtga ttggcttggt ttgtagtcta ttagtagatt atggtctcag
    20581 tcaggttcaa tttcttcttt atgatcattt ggaaaataat atttattctt ctccgtttaa
    20641 attattcaaa cagagttggc aattgatgaa aggtaataag tggcgtcgat tcatgcttga
    20701 cttgtcattt attggttggt tcatcggtat tttactgact tttggtttgt tggccttgta
    20761 tgtttatcct tattattgga cctgccaagc cttgttttat gaggatttga ttgccaaaaa
    20821 tcctttgtta ctttcgaagg tagattccgt ttggttttcg gaactaggat aagcatagat
    20881 tttatacagc tacattgaaa taaaaataca gacagtttct gctggattta gcgatcagca
    20941 agattctgtc tgtatttttt atcatcattg ctaaagttca agttggtttg atggtgatgt
    21001 gaaatagagt tcatatacca accaagtaat cataccagct tggataagga ggctgataac
    21061 tgagccttcc acaccaaatg caccacctga caaccagtct ggaccagagg tttctactgc
    21121 gataaaggct tggccagctt gtgtcccact cactggaaag gcaaagacat ttccttggaa
    21181 gcagttccat gcagcgtgga taccactgat gacccagata ttgcctgttt ttaacatgac
    21241 cagactggct agaatggcaa agaggggcaa gtctaggaga ggaatgaggg agatggcatc
    21301 atttcccaag tggagaaagg tgaaaaagag ggaggaaaca aggataccga tttttcgtat
    21361 tccaagccca agccaaggac ttttttctac aaatcttgcc cagagtatga tggcaagact
    21421 gatgaaggca aaggccaata attctagact gagtgtcaca aaggcattat ctatgaaaat
    21481 gaaaaataag ggcataaagg caaagtaacc aactgtttca gctactgcca tcaagaccag
    21541 tgctgtgata ggcgatagga taaaattgag attaaagcgt gatttggctt tctctgaaat
    21601 gaatgctgct ttcatatttt tctcctagtt tgtgtttagt accagtctat catattttcc
    21661 tatggaaaac tagacatcat tttgataagc atgatataat gatacaaaag tatagaagga
    21721 gatacatcat gcaattacag gattggctaa gagattttca atttggagaa caccaattgg
    21781 taggtccatt tttctatgca accccgctcg ctttgaaatt tgaagttggt cctgcagagg
    21841 aggctgaaac ccttcctaag aaagtttatc tggatcgagc ttatgcgcgt gcggtagagc
    21901 ttttaggaca ggccagctta gaatatgact atgtgattct ctcgctactt cgtcaggaag
    21961 atagggacat cgacacttac ctctggcatt ttacatcgaa atttaacttt aacaagtgtc
    22021 ctgagcctga gttgatagaa gtggaggact ggacgggaga agtgctagtc tttgaacgct
    22081 atctttttcc ggtcgctgac caagatttga aagatctttt gaaggagatt atcagggctg
    22141 atcacggtgg tttcaactac ctatccagct ccgtcctctt cctgtctagt agggacaagg
    22201 tcatttatca ttgctatgat gataggggag tggacgtcgc tgtggtggat gatgacaagc
    22261 gtcgtcagct ctttacggat tgccaggacc tcctttttga ctatgatatg gaggagatgg
    22321 tgaggaggat ggagagctag aaatccttgt ctaaagatgt tatactacat ctggaatgaa
    22381 catttgaagg agattttatg gaaattgata accgactggt tctcttagga gatcagttaa
    22441 aagaacattt aggaaaaggc agtaaagtta agatggctgt agcaactttt tccatgtttg
    22501 cttatcagtc tttaaaagaa gagcttgagc aaattgacga gctgaaattt atttttacca
    22561 gccctacctt cacgaccaac gaagtcccaa agaataccgc gaatacacca taccaaagaa
    22621 acagcgagaa tcatctatat ttggagctga gtacgagctc aaattgatga acgagctgac
    22681 ccaaaaatct cttgccagag aatgtgccgg ctgggtccgt cgaaaggctc agtttaagtc
    22741 tatcaatgtt catgaagaaa ttgacaacgg cattcggatt gaaaacgatc aaatcatcgc
    22801 agttgataag ctgaaaaatt ttgaccgtaa ggaactgggt tatgaaaatt ctttgtttaa
    22861 gtccagccgc aatttgtata cggaaccaca aagtttgacc tatctgcgaa actttgaaga
    22921 ctactgggaa aatgatgaat acttccgtga tgtgaccgag gaagtattgg aaaatctgac
    22981 cttgccccat aaggaacatt cgccagaaat cctctactat gtcatgcttt ataatatctt
    23041 tagtgagttt ttggaagacg ttaatcagga ccaactgcca aatgaacatg ttcaatacaa
    23101 ggaaaccaag atttggaatc tcttatatga ttttcaaaag gatgcggtca agtcgctgat
    23161 ttcaaaatta gaacgataca atggctgtat tctggcagac tcggtcggtc taggaaagac
    23221 ctttacagct cttgctgtca tgacctacta tgcctatcga ggcaaacgga tcttggttct
    23281 atgtcccaaa aagttggaga acaactggaa tatgtaccga cacgactatg tcaacaaccc
    23341 catttacgac cgctatctcc agtatgatgt gctctaccat acagacctca gccgtgacaa
    23401 gggaacatca aatggaattg atttggcact taatcgttgg gatacctatg atttggtggt
    23461 cattgatgaa tcacacaatt ttagaaatgg tggatcatct gatgccgaga ttgaagaagg
    23521 tcgtgaaaat cgatactccc gcttgatgaa tcggatcatc aaaagcggtg taccgacaaa
    23581 ggttcttatg ttgtctgcta cacctgtcaa taatcgcttt aatgatttga aaaatcaaat
    23641 tgccctagcc tatgaaggtg atgcggcaca atttgatgcc aaattagata ccaaatcctc
    23701 catcaatgat atcttccgta tggcacaggc tgcttataat caatgggcag agctaccagc
    23761 agaatcacgg acgacagcag ctttactaga tagacttgat tttgatttct ttaaagtcct
    23821 tgatagcgtg acaattgccc gtagccgtaa gcatatccga caattttatg atagaaatgc
    23881 gattggtgat tttccagaac gactggcccc taaaaatttc tatccagact tgacggtcaa
    23941 tcgaagagac attacgtacg aacggattta ttctctattg gatcagctac aactgaccat
    24001 ttatcagcca agtcttttca ttcatccttc aaaacgttcc cgctatgaaa aagcggaagg
    24061 gcaaggtttg acccagcttg ggcgggaaac ggggattaag aaactgatga tgatcaacct
    24121 cttgaaacgt ttggaaagtt ctattgaggc ctttcgctat accttgattg acgtggttaa
    24181 ggcttatgta gacaaaacaa ttctgagcat agaagagttt gaagttgcag gtggtgaggg
    24241 aagtgttcag ttgcaagagt tggctgaaga tgattttgat gtcgaagata gcaacacgga
    24301 tttctttgtt ggaaagaaat tccctatcaa gctggctgac atggattacc tatcttggaa
    24361 gagggattta caggctgacc aaacagtgtt gagggaatta gaagacctgg tcaatttaat
    24421 cacaccacag gaagatcaga aattacagac cttgtttaac ttgattgatg acaaattggc
    24481 acagccaatc aatccagata acaagaaagt gattatcttc tcagcctttg cgacgacaac
    24541 agactatctt tataaaaata ttagccatta tctcttggat aaatatggac ttcatacagc
    24601 ccagatttct gggacaaaag gtttttcgac cactctgcca cttaaaaata aggacttgaa
    24661 caccattctg acctatattt caccgagatc caagcagaag gacttgcttt atcctgatga
    24721 tcagcgggaa attgatgtct tgattgcgac agatgttatc tcagaaggtc aaaacctgca
    24781 agacgctgat accatgatta actatgacat tcactggaac cctgtccgta ttgtccagcg
    24841 ctttggtcgt gtggaccgta tcggtagtca gaacaaatac atccagctgg tcaatttttg
    24901 gcctaacatc gctttggata cttatattga tctcaaggcg cgtgtggagt ctagaatgaa
    24961 aatttctgta ctgacttcaa cggctgatga caatgtcttg accaacgagg aaatcgagga
    25021 gaacaattat aggaagaaac agcttgaacg actccagaca gaggtcgttg atttggaaga
    25081 tatgaatgag gggatttcca ttatggatct aggattagaa gactaccgta tggacctgct
    25141 caactattta gccaaacacc cagagttaga ggcaatgcct aaaggtcttc aagcagtggt
    25201 gcaggcaggg caagggcagt cagctggagc catcttcctc ttaaagaata ttgccaacca
    25261 gccccatctc agtaggaaaa atcgtcttca cccctactat ctgatctata tcagtgacca
    25321 gggagagacg gtctactcca ttgcggatac caaggcattg ctggagcacc tacgcatgat
    25381 taccaagggt aaacatcagc ctgagaaaga tttggtggca acctacaatc gtgccactga
    25441 tgatggcgcc aaaatggagc attactccga cctcttgcaa agaagtcttg aaaatctcgt
    25501 ggagagcgat gaggagacct tcaatgagtc cctcttcaca tcagatacca ttgactttat
    25561 ggatagcggg gtggacggaa gtgatgattt tgaactgctg gccttttttg ccgttttgaa
    25621 aggagaagac taagatgcca tttttacctt ctactcagat tccaagaccc aaagtcctct
    25681 acaagcctta tcaaaaaggc aacaaggact ttctagataa ttttggtctg tctacagctg
    25741 ataagcagct cctccggcaa caaatcggcc agattaccgc tacccatcaa ttagatgcca
    25801 agaccctccc cattccagcg ggcaagacca ttcagcaaat catcgtcctt cggattgacc
    25861 ttttgagtca gcaactagac ccgcacttac tagcagagct ggacacttat ctgggatttt
    25921 acactctctt tcatctggta tttccagacg ggactagcca gtatttgatt cattttaagg
    25981 aaaagctggc gcaatcgaga gagggacgga attttaagat tgttcgtcac tttcaaacag
    26041 acaaacctct ggcattgacc taccaagaaa gggacttgga ccagttttat gaaaacttgg
    26101 ttaaacaagc gggggaagaa gaactggtcg ggggcggagc ttctgtcaaa gaccagattg
    26161 agcagacgga acgcttagca gcaatggaaa agcaagcagc tcagctcaag aaaaagatgt
    26221 ttgccgaaaa agtcatgcgc aaacaaatgg aacttcgaaa ggcctacaaa gccctagaag
    26281 aagaaatttt acaactaaaa aatccacagt aaattctgtg gagaatatag tataatagaa
    26341 cagtagaaaa gttccaaaag cggggcggaa atctattttg ggaggtggtg tctatgcagt
    26401 atcgaactag aaaggtttgc cgcaatgtct gtcgcagagg cccttgggct gatgtttcag
    26461 tttggcacgt tactaatcgt gctactgaca tttattttta acaatcgtaa aaaataaaag
    26521 acagccaacc gctacgaaac tttgaccgag tgaactagca ggttgactgt aatcttcatt
    26581 cgtcccgtct ttaaaacggt tctactggga ttgagttggc gctcaatccc tttttcaccc
    26641 ttattatatc acaaactaaa atcgtttgac aattttttat ctacatcatc aaaaaaggct
    26701 tgacagccta agaaaaggag attaccat
//
LOCUS       DATUCG010000015        25210 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.015, whole genome shotgun sequence.
ACCESSION   DATUCG010000015 DATUCG010000000
VERSION     DATUCG010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25210
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.015"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(89..733)
                     /locus_tag="VCA20_000513"
                     /pseudo
     CDS             complement(89..733)
                     /locus_tag="VCA20_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942636.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
     gene            complement(1054..1719)
                     /locus_tag="VCA20_000514"
     CDS             complement(1054..1719)
                     /locus_tag="VCA20_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEP1834370.1"
                     /translation="MPNYTIGILGLGVFGTTIAKTLHNYDCNIIAIDNHEQRINHLEP
                     ILTRGIVGDITDRSLLRAAGIGNCDAVVVATGENLESSVLAVMHSKVLGVPMVIVKVK
                     GTTAKEVLLRVGADKVISPERETGISLAKQLLHRDTTSLFELEGNVSIVEFHPPVKWI
                     GKTLGELKLRQHYKLNIIGYRSGENQELNIQLTPDYVFKSDELILAVTDHNTVDHFEE
                     LTN"
     gene            complement(1731..3080)
                     /locus_tag="VCA20_000515"
     CDS             complement(1731..3080)
                     /locus_tag="VCA20_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEP1834371.1"
                     /translation="MSPFRIKLSPSQRIIISFLFVILTGSILLSLPISQISTSTASYW
                     DHLFTSVSMVCVTGLFTKAVAETYSTFGQIICMMLIQIGGLSLIGFIGLFALRGGRKM
                     NFINMATLQEALNRSDTKYFRNFLKSAFGFTLAVEAFGALLLSFHFVPEFGWARGLFT
                     SIFVAISAFCNAGFDNFGATSIMRYVDNPLINITLASLIIMGGLGFSVWFDLQTQIGQ
                     KRSLRKLGFHTKIVLGLTAIILTLGTLTTLLTEWNNPSTIGQLSFGHKLLASFFQTVT
                     MRTAGFASIDYTKAEPITLLLYIFQMMLGGAPGGTAGGIKITAFLTLVLYARSEILGL
                     PHTNFMGRTIDNLSIRKAFATFSVFLLMFIIGLFALALTDDRQPLLFLMFEVMSALAT
                     VGVTANLTPSLTMAGQAVIMALMFFGRIGPLSILASLSVRKLSKTESLQYAKSSMLV"
     gene            complement(3343..3882)
                     /locus_tag="VCA20_000516"
     CDS             complement(3343..3882)
                     /locus_tag="VCA20_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HEP1834372.1"
                     /translation="MNSKRQTLNFITMLAAQAVVISLIERLITPPFAFAPGAKLGLGN
                     LISLIAIFTLPTKDSLKVVALRLLISTFLGGTFSTFLYGFAGTTLSYVGMLSAKQLGP
                     NRVSPIGISILGGMLHNLGQLLVFATIARSFYVLNYLPILAFTGILSGLLVGLAATYL
                     LQKVGPLRHYHQLVLAEWK"
     gene            4066..5958
                     /locus_tag="VCA20_000517"
     CDS             4066..5958
                     /locus_tag="VCA20_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEP1834373.1"
                     /translation="MTKNIVIVGAGYAGIAAARLLGKTFKKDQDVTVTLIDKNSFHTY
                     MTELHEVAAGRVEANAIKYDLQRIFKKYPKVQLVTDKVVEIDYDKKQVVAEHQILDFD
                     YLLLAMGGEANDFGVKGVKEHGFTLWSIEAAKRLHDHMIDACYRAMRELDEAKRRALL
                     TFTVIGAGFTGIEMIGELIDWVPILAREFKLDPKEFSLKVVEATPNILAMVTEKEQVK
                     ARKYLEKKGVELVLGDGVASVQEDSLTLSSGRQIPTYTSIWTAGVQANTDASEFGIEK
                     ARAGRLVANEFMEAKGKENVYVAGDLVYFEESEGKPTPQIVQAAEQTGHTAASNIIAA
                     IKGGEKHSYKGKYDGFMVSIGARYGVAFLMDKYHMSGFMAMAVKHMVNLLYFFTIRSF
                     FYMGCYVRHEFFDIQNKRNIFGGHTSGKGNLLWSVPMRVLYGSVWLYEGIKKAFGLFG
                     TTSWFGDQVVFPFPWLADPVSGASAAEAVSSASQAATAAAEVAEPIFGLSYAYGEAPM
                     AVLDKMPDWFATIMEFMMPNQEVALFMQKFMTVAEIGIGLALIAGAFVWIVSAATVAL
                     VVMFSLSGMFYWVNIWFIPAAISLMNGAGRAFGLDYWIMPWLGRFLDKKIYGKPKHIY
                     RIKDKK"
     gene            5977..6966
                     /locus_tag="VCA20_000518"
     CDS             5977..6966
                     /locus_tag="VCA20_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEP1834374.1"
                     /translation="MVHQIWNTYPEIERGLEEVKSIILSEMTVLHPAVTSKIVEYVEA
                     PGKYLRAGLCLLFAQMTEGKISRSKLYFAAHLEVLHLATLIHDDVIDGADKRRGVTAA
                     HQQFSNRIAIYTGDYLLAYSGRLLGKGLRLLEVSRGDLDLGNDKLMETILRGELSQLL
                     NQFDANMTMKAYLKQIQGKTAFLFGLACQLGSFVPGQSRKDSSMAFRSGQALGMAFQL
                     RDDLIDYQLDVAESGKPRLQDIQNGIYTAPLLFAMREDKSIQQQLRAYIQSPTAEGLE
                     ILIDRIFATHAIAKTEKLIVSYLEKMMSFADRIPQLSTASMQNLVAIVFSHYF"
     gene            7045..7524
                     /locus_tag="VCA20_000519"
     CDS             7045..7524
                     /locus_tag="VCA20_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HEP1834375.1"
                     /translation="MKTTKVVLKSLVVLGAVFALAACGSKQESTSTSSSTTETVVLKD
                     GTYKAESGKDDYGYKIVHTLTVKDGKISESKFDYEAEDGSLKSANEEYNKNMKDKAGV
                     SAGEAIEQLNAALVEKQDLSAVEVVSGATHTSEDFKKSTEALLAAAAEGKTETVKLD"
     gene            7602..8651
                     /locus_tag="VCA20_000520"
     CDS             7602..8651
                     /locus_tag="VCA20_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEP1834376.1"
                     /translation="MKKIASLLFVGLLMVLLVACGGKQNEGLSVVKTPLTRSESLLHT
                     VVQLSIYHENQEAAMDEAVSYIKEMESLLSTNLEGADVYRINQAAGKEAVKVDERTFE
                     VIETAIDMSKESQGLFDISIGAVSNLWKIGDEDARKPSDEEIKAALPFINYKDITLDK
                     EKKTVFIKEGMTLELGAISKGYIADKVRELFASKGITTAIINLGGNVVVMGDSPTTQN
                     GWNVGVQDPDQVRGSTVGSVPGTHDSVVTSGIYERYLEVDGVKYHHILDPKTGYPVEN
                     DISGVTVFSKTSIQGDAFSTTLFLLGVEKGLDFINQIDGVEAVFIDKEQGVHLSDGLR
                     ESFELTNEEYHLVNE"
     gene            8644..9600
                     /gene="menA"
                     /locus_tag="VCA20_000521"
     CDS             8644..9600
                     /gene="menA"
                     /locus_tag="VCA20_000521"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HEP1834377.1"
                     /translation="MNKSTKGLSLPVFLEFVELKTKVASVFPMTLGILWSLYRYQAFN
                     WLNTLLFIIAVLSFDMCTTAINNSMDYHKAKDETYRQESNVIGKFSLDFRQMIGIVLA
                     LLIFSLLVSLLLVWRTSWLLLPMGALCFLIGIFYTFGPIPLSRMPLGEVFSGVTMGFG
                     IFFLAVFIQQPDLLLTSQVSGQWMTLQFSWTKIIEIIFMSIPLVTLIANIMLANNTCD
                     LEEDIRNHRYTLVYYIGKKNALKLYFVLASLPWLLWIIYCLIGFLPIWALAGLIGVWP
                     AYKSLETFLKKQVKRETFIEAVKSFVLFALLYVVILALALIF"
     gene            complement(10008..10436)
                     /locus_tag="VCA20_000522"
     CDS             complement(10008..10436)
                     /locus_tag="VCA20_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HEP1834378.1"
                     /translation="MTFKSILKQLKLFDYILIGFTLVLSFLPAIFTYTHLTTDANEAK
                     TIAYVRINGEVVDQFELSKDTPHQEKTYYPNEGQYNIIEVDGERIRVKEDNSPDQIAV
                     MTSWISQPGQLSVCLPHNLLIEIKSVGGESTDEEELILPL"
     gene            complement(10489..10836)
                     /locus_tag="VCA20_000523"
     CDS             complement(10489..10836)
                     /locus_tag="VCA20_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834379.1"
                     /translation="MNVNKPKLILTLIVLSYTAICSLYIHSYLTGDNPPIFILLFTIL
                     PILNSDWQTSGPEANAFQKAIYLYHKPLLWFLFAGFALTFSIVFFAGKIQPLHAGFWG
                     MLAILLFFRQLKK"
     gene            complement(10826..11170)
                     /locus_tag="VCA20_000524"
     CDS             complement(10826..11170)
                     /locus_tag="VCA20_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEP1834380.1"
                     /translation="MNKPFTPKQTCRFFVLFYAIFSAFFIYSYMVNEPISGSFLLTLP
                     TIQIIKSYQTAEEKRYFIIQAIFALFAMTLTYSVGIWLGHVKPHILILPAGFAVAILY
                     RLYFKKEKTDER"
     gene            complement(11183..12265)
                     /locus_tag="VCA20_000525"
     CDS             complement(11183..12265)
                     /locus_tag="VCA20_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3278 domain-containing protein"
                     /protein_id="HEP1834381.1"
                     /translation="MKKETFQDKLIKRFYGIAGPLDEFRQKEAFRLGNTCFILLFWGT
                     MALTLLALALSKRYPEVVAYGYPVTLLLAYLSASTYIMFKLRHSQLDSLDVEELTTKE
                     QKQLKGASIKFALYFTTVMYIWTVVFEAWMEGLNPLDHLFDLRKFLAACLGGVFTGIA
                     IEIMLRKRMKKAEQLSVSSAIAKEEPKWIKNMIRHFYGIRGPLDEYRRTKADEIGGIA
                     FIYHFYFLALGNAIAYFLAIRYPVEVANYYPMIIALFSFILLGVVNMQTLHADLPQYD
                     LDELSPEERQGRPLNPILWGLGVALLSTIFAGVADLFSLNLPLTASILHTKSLLFGGT
                     MGIFASIALSAFAYLHKLEAETTKKK"
     gene            complement(12276..12476)
                     /locus_tag="VCA20_000526"
     CDS             complement(12276..12476)
                     /locus_tag="VCA20_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834382.1"
                     /translation="MKKNIRQALISTLIFIPLFVLFQLWGNSGAEILHTISQAKFWLQ
                     VLIAGTIYFLGFVYILPLFDQK"
     gene            complement(12491..13024)
                     /locus_tag="VCA20_000527"
     CDS             complement(12491..13024)
                     /locus_tag="VCA20_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3278 domain-containing protein"
                     /protein_id="HEP1834383.1"
                     /translation="MKDLVTKLLNQATKDFYSISGPMDEMRRQESYRLSNTIVMIVFP
                     ILVIGNTIALLIALRQPEKVGFLLPLTNILIIGFTQALASHLALKNGISQSYEDEIDV
                     TKLNKSLVKSSRSTFFGAFLASTTAPAFYKEWSVFLEELTDPILLLGRLIVAGAITFI
                     HYYYCKKQLQKKILANK"
     gene            complement(13035..13223)
                     /locus_tag="VCA20_000528"
     CDS             complement(13035..13223)
                     /locus_tag="VCA20_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP1834384.1"
                     /translation="MNRVKDYRLLLGISQLDLAKAISVSRQTINMIENNKYNPSLDLC
                     INLAKALQTDLNSLFWNE"
     gene            13494..14444
                     /gene="rsmH"
                     /locus_tag="VCA20_000529"
     CDS             13494..14444
                     /gene="rsmH"
                     /locus_tag="VCA20_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEP1834385.1"
                     /translation="MTKEFNHTTVLLHETVDMLDIKPNGIYVDATLGGAGHSEYLLSQ
                     LTDGGHLYAFDQDQTAIDHAHIRLASYIEKGQVTFIRDNFRNLKTRLAELGVTEIDGI
                     CYDLGVSSPQLDERERGFSYKQDAPLDMRMNREGHLTAYDVVNNYDYHDLVRIFFKYG
                     EDKFSKQIARKIEQVRAVKPIETTTELAELIKSAKPAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAIDLLALDGRISVITFHSLEDRVTKQLFKEASTIDVPKGLPFIPD
                     DLKAPLELVNRKPILPSQEELEANNRAHSAKLRVAKKVHK"
     gene            14455..14778
                     /gene="ftsL"
                     /locus_tag="VCA20_000530"
     CDS             14455..14778
                     /gene="ftsL"
                     /locus_tag="VCA20_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEP1834386.1"
                     /translation="MLQEKRREATMRVIGDKIRTFTRVEKAFYSSIVLSGLALAIGII
                     FMQTRLLQLQSGMAKVNQDISQKQIEINDAKQAVNELTRSARLMEIAEKAGLTFNNDN
                     IGVAE"
     gene            14778..17027
                     /locus_tag="VCA20_000531"
     CDS             14778..17027
                     /locus_tag="VCA20_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEP1834387.1"
                     /translation="MPRRNNKLLRYVLKKRYIPSKNRRRVGQSLLLLTVFVFFLFLIN
                     FAYIIGTDSKFGVDLSERADAVHQREVTVQAKRGTIYDRTGIPIAEDSTTYTVYAIID
                     TEYVSELKKVLYVQSSQFSKVAEIFKKYLGMETDYVMEQLNQPKLKQVYFGTLGKNIP
                     YSTMSSIQEEMKAAGIEGIAFDASPGRMYPNGNFASIFVGLANPVENKDGSYSLQGVS
                     GLEYYLNDILAGQDGKVIYEKDSQGRVLPGTEKVETETVNGQDVYTTISADLQRSLES
                     NVNTFFDNAKGRYANATLVSAKTGEILATTQRPSYDSDTKEGLGQEGLLERSLLYQEA
                     FEPGSTMKVLTVASAIDNGTFDPNAYYTNNEYVIADAKINDWTLNAGYSAVTLNYAQG
                     FSLSSNVGMTLLQQQMGDEKWLNYLAKFRFGYPTRFGMGDEAPGMVPEDNIVSIAMSS
                     FGQGISANQAQMLRSFTAIANNGIMLEPKFISALYDPNTDTARVSSKEEVGNPVSEQA
                     ADDTLRYMINVGTDPVYGTLYSHDLQGPAIQVDGYDIAVKSGTAEIASEDGQGYIKGA
                     YINSVVAMIPAEDPEFIMYVTIRQPEVLNVLSWRDIVSPTLKEAMLLKDNLNLDSPAP
                     ALAQVVNETEYQLPDLIGKAPGESAEELRRQLVQPVILGNGAEIKKVSVEKGSNVIAN
                     QQVLLLTNKFKEMPDLYAITKENMDIFAEWTGIEVAYKGTGSKVVDQSVEVGTALNKI
                     KKITITLGD"
     gene            17029..18027
                     /locus_tag="VCA20_000532"
     CDS             17029..18027
                     /locus_tag="VCA20_000532"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEP1834388.1"
                     /translation="MQFALMSGLVAFLATVLLIPRFITFYQAKRIEGQQMHEDVKQHQ
                     FKAGTPTMGGTVFLVVAILVSLLFATAFQLLTGGVLAILFILALYGVVGFLDDFLKIF
                     RKINEGLNPKQKLALQILGGIVFYFVHVRGAGGGELNVFGHMVHLGVLYFPFVLFWLV
                     GFSNAVNLTDGIDGLASISVVISLLAYSVIAFNEQKFDILLVCVTMIGGLLGFFVYNR
                     KPAKIFMGDVGSLALGGMLATISIALRQEWTLLLIGLVYVIETSSVMLQVSYFKYTKK
                     RFGEGRRIFRMTPFHHHLELGGLTGKAEKWSEWKVDFFLWSVGLIMSLITLAILYL"
     gene            18109..18507
                     /locus_tag="VCA20_000533"
     CDS             18109..18507
                     /locus_tag="VCA20_000533"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834389.1"
                     /translation="MEQFNQEQLYGVVIQTMTASSQLPMVKVNREEFLKTRFKDSPYL
                     DKIIADGPTAVFTTKALRKEAEKIINDMTTKTSLISFAAGLPGNPFTAVAAGTADIAQ
                     YFGFALNLSQQIAYLFGEDELFVGEGKEIS"
     gene            18678..18941
                     /locus_tag="VCA20_000534"
     CDS             18678..18941
                     /locus_tag="VCA20_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIB"
                     /protein_id="HEP1834390.1"
                     /translation="MIGVKITKKTVEKTIANAVPILGGLISGGLTYFTFQPMGGMLAD
                     TFVKKINGDFDAGLELTEAFKASLEEEKELGIIEAEFTEASES"
     gene            19021..20364
                     /locus_tag="VCA20_000535"
     CDS             19021..20364
                     /locus_tag="VCA20_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEP1834391.1"
                     /translation="MKFTDMKLKPYIQEALKEINFVQPTEVQEKLIPVVLSGRDLIGE
                     SKTGSGKTHTFLIPIFQKLNEELDQVQAVITAPSRELATQIYQAARQLASHSDIEVRV
                     TNYVGGTDKNRQIDKLQASQPHIVVGTPGRIYDLVKSGDLAIHKAKTFVVDEADMTLD
                     MGFLETVDKIAGSLPKDLQFMVFSATIPQKLQPFLKKYLSNPVMEQIKTHAVISDTIE
                     NWLISTKGRDRNAQILEVTKLMQPYLAMIFVNTKTRADELHSYLTANGLRVAKIHGDI
                     PPRERKRMMNLVKNLDYQYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLSGIAITLYQPSDDADIRELEKLNIKFLPKEMKNGEFVDTYDRDRRANREKSREK
                     LDLEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKTKRVEARAKGRAERKAKRQTF"
     gene            20382..20606
                     /locus_tag="VCA20_000536"
     CDS             20382..20606
                     /locus_tag="VCA20_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HEP1834392.1"
                     /translation="MLQNILSFYLQGVLIASLLIITSSLVWFIWRATKGVDKTLQERQ
                     DFLFDLLMINVMTIPIAAFGVVGILLMFKA"
     gene            20620..21537
                     /gene="trxB"
                     /locus_tag="VCA20_000537"
     CDS             20620..21537
                     /gene="trxB"
                     /locus_tag="VCA20_000537"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEP1834393.1"
                     /translation="MYDTVVIGAGPAGMTAALYAGRSNLKVALLERGIYGGQMNNTAE
                     IENYPGYDHISGPALAEKMFEPLEKFGVDHIFGTLVRIEEEGQIKKVITEDGVLETKT
                     VVLAMGAKHRLLGIPGEDTYNSRGVSYCAVCDGAFFRGQKLLVVGGGDSAVEEALFLT
                     QFAESVTIVHRRDQLRAQKVIQDRAFANEKINFIWDSVVEEIKGDDLRVQSVVIKNVK
                     TEEVSELDFGGVFIYVGLDPMTDTVADLGITDEAGWVITNEKMETTQSGIYAIGDIRQ
                     NQLRQIATAVGNGAVAGQEVYNYITELAE"
     gene            21547..22086
                     /locus_tag="VCA20_000538"
     CDS             21547..22086
                     /locus_tag="VCA20_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEP1834394.1"
                     /translation="MFHIYDIQKNPDGISFDKTLDLQEELQARNGEVLGLSPVQVTGN
                     IRFESGFFFLDYQMTYDITLASSRSLQPVVLHEVQNVNELFVANEAVLKEQDLIDEDM
                     VLVVEDDHIVLDESVADNILLAIPIKVLTPEEEARQELPSGKAWALMTEEDFQQKAQE
                     KKEANSPFAQLQGLFGDED"
     gene            22183..23610
                     /gene="gndA"
                     /locus_tag="VCA20_000539"
     CDS             22183..23610
                     /gene="gndA"
                     /locus_tag="VCA20_000539"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEP1834395.1"
                     /translation="MTKANFGVVGMAVMGRNLALNVESRGYSVAIYNRSADKTEDVIA
                     SNPGKNLVPSYDVESFVASIEKPRRIMLMVQAGPGTDATIQALLPHLDEGDILIDGGN
                     TFYEDTIRRSKELANSGINFIGTGVSGGEKGALEGPSIMPGGQKEAYELVADVLEEIS
                     AKAPEDGAPCVTYIGPDGAGHYVKMVHNGIEYGDMQLISESYDLMQHLLGLSVDEMAD
                     IFTEWNKGELDSYLIEITADILKRKDDQGQDGPIVDYILDAAGNKGTGKWTSQSSLDL
                     GVPLSLITESVFARYISTYKDERVAASKVLPKPTPFAYEGDKAELVEKIRQALYFSKI
                     MSYAQGFAQLRVASKENNWNLPFGEIAKIWRAGCIIRARFLQKITDAYGRDEDLANLL
                     LDEYFLDVTAKYQQAVRDVVALAVQAGVPVPTFSAAITYFDSYRAENLPANLIQAQRD
                     YFGAHTYNRKDKEGIFHYDWYSESK"
     gene            23692..24381
                     /locus_tag="VCA20_000540"
     CDS             23692..24381
                     /locus_tag="VCA20_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEP1834396.1"
                     /translation="MAKKILIAGKERNLSHFVSMELQKKDYLVDYASTGKEAMSLAHE
                     TDFDLILMSFQLSDMSSKELATELLAIKPATVMIVVVEPTEVSQYGEEVLSYAVSYVV
                     KPFVISDLVEQISAIFRGRDFIDNNCKQVHMHAAYRDLKVDFQNRTVTRGDELINLTR
                     REYDLLATLMNSPEPVSREQLLERVWKYEAASETNVVDVYIRYLRGKLDLPHQDSYIK
                     TVRGVGYAMRD"
BASE COUNT         7521 a         4594 c         5821 g         7274 t
ORIGIN      
        1 cctatcaaca atttgaaatt ctctgagttt aattttaagg ctttttcgta tgttgtttcc
       61 atagctttag tataccactt ttgagttacc gaacaagtct aatgtaaaaa ataagaaaga
      121 ggaccgaaca ggcttgagca aagatagttg caaagccagc tccgaaaacg ccccagttca
      181 taactaagat aaagaaaaaa tccagaccga tattgataaa acaagcgata actagagcca
      241 gtagaggtgt tttagagttt ccgagactgc gtagggctga agacaggaaa ttataaaaaa
      301 tagtaaaggt caatcctcca aacatgggcg ttagaaaggc atgggagtgg tcgatgatac
      361 tcacaggcgt ttgcattaag accaagatag gtttcaaaaa agctaaggct gagactgtta
      421 gtaaaaggct gatgagggaa gcgtagaaga gtccgtggac aaaagatttt ttaagcccct
      481 ctaagtcgcc tgctccaaag cgttgggcag aaataatggt taatccattg gtaacacctt
      541 gggcaaaacc gatgattaaa aaaatcaggc tggctgtcga accgacactt gcaaaggcgt
      601 cctttcccaa agtatgcccc acaatcatgg aatcagcaaa attataggcc agttgaaaaa
      661 aagagccaat caacaagggg atagcgaatt gtaagatgac cgcaatcggc tttcctttgg
      721 ttaaatcatt cataacgtct ccttttgaca gatgataagc ccactagaaa aattatagtc
      781 tagtaggctt tctagggata aaatatttct cctgaaaacg gagtataggg cgtaaatttg
      841 ttatttatac tcttcgaaaa tcaaaatcag acgttgttga cttgatttga tgagtgaaag
      901 gctccagtgg agcctttcag cctgttacct tgaaacaaaa tagtaacagg gttctatctg
      961 cgtaagtagt aacgaactac gttcgcccta tctccaacct ccaaaggttc cccaaacctt
     1021 tggagctagt ctgtttttga ttttcattga gtattaattt gtcagttctt caaaatgatc
     1081 gacagtgtta tggtctgtta ctgctaagat gagttcgtca gatttgaaaa catagtcagg
     1141 agtcagttgg atgtttaatt cctgattttc gccacttcgg taaccgataa tattgagttt
     1201 atagtgttgg cggagtttta attcgccgag tgttttgcca atccatttta ctggcgggtg
     1261 aaactcgaca atggaaacat tgccttcgag ctcaaagagg ctggttgtat cgcggtggag
     1321 taattgtttt gctagagaaa tacctgtttc acgttcagga gaaataacct tatcggcacc
     1381 gacacgaaga aggacttctt tcgcagttgt tcctttgact ttgacaatga ccataggaac
     1441 gcctaggacc ttgctatgca taacagcaag gacactggat tccaagtttt cgccagtagc
     1501 tacaacaaca gcatcgcagt taccgattcc tgctgcacgt aggagcgagc gatcggtgat
     1561 gtctccgaca ataccgcgcg tgagaatagg ttctagatga ttgatacgtt gctcatgatt
     1621 atcaatagca atgatattgc aatcgtaatt gtgtaatgtt tttgcaatgg tagtaccaaa
     1681 aactcccaag ccgagaattc cgatagtata gtttggcatg atttctcctt ttaaacaagc
     1741 atagatgatt ttgcgtattg aagactttcg gttttagata attttcgaac agaaaggctt
     1801 gcgagaatag atagtggacc gatacggccg aagaacatga gcgccataat gactgcttga
     1861 ccggccatgg ttagacttgg agtcagattt gctgtaacac ccacagtggc tagtgctgac
     1921 atgacctcga acattaaaaa tagaagtggt tgtcggtcat ctgttagggc aagtgcgaag
     1981 agcccgatga tgaacatgag aaggaagact gagaatgtcg caaaagcttt gcggatgctg
     2041 aggttatcaa tggttcgtcc cataaagttg gtatgaggga gaccgagaat ttcactacga
     2101 gcatagagta ctaaggttag gaaggcggtt attttaatac cgccggccgt tccacctggg
     2161 gctccgccaa gcatcatctg aaagatatag agcaagaggg tgattggttc agccttcgta
     2221 tagtcgatgc tggcaaaacc agcggttctc atggtgaccg tctggaaaaa gctggctaat
     2281 aatttatggc caaatgatag ttgtccgata gtagaagggt tgttccattc ggtgagtaag
     2341 gtagtcagag ttccgagggt cagtataatg gctgttagac ctagaacaat ctttgtatgg
     2401 aaccctagtt ttcgtaggct acgtttttgc ccgatttgtg tctgaaggtc aaaccaaacc
     2461 gagaatccaa gacctcccat aataatgaga ctggctaatg ttatgttgat tagaggatta
     2521 tccacatagc gcatgatgct agttgcgcca aaattatcaa aaccagcatt acaaaaggct
     2581 gatatagcta cgaaaatcga ggtaaataag cctctcgccc atccaaattc aggtacaaaa
     2641 tgaaaggaca gtaagagggc tccaaaggct tcgaccgcca aggtaaaacc aaaagctgat
     2701 ttgaggaagt tacgaaaata ttttgtgtca gatcggttta gagcctcttg taaggtcgcc
     2761 atattgatga agttcatttt acgaccaccg cgtagagcga agagaccgat gaatcctatc
     2821 aagctcaaac caccaatttg gattagcatc atacagataa tctgtccaaa agtagaatag
     2881 gtttcagcaa cggctttggt aaatagccct gttacacaaa ccatggagac agatgtgaag
     2941 agatgatccc aataacttgc tgtagaggtg ctaatttgtg agatgggaag acttagtaaa
     3001 attgatccag tcagaatcac gaataagaaa ctgataatga ttcgctgact gggagaaagt
     3061 ttgatcctaa atggtgacat aattctacct tttctcaaaa aaataaaaga tgaagatatt
     3121 gtagcacaaa agaaagaaaa tgtacgacta aaacacgcaa agaacctgat tttttctctt
     3181 catgaaagct tcattatttt ttattaccat ggattttata agtggtgttt atatgaggtg
     3241 gctcattata tttgtgacac caggattatg gggaaattat tatgggcaat gaaaaagtgg
     3301 tgcggaccat aaaaagaatc tccttagttg acctagggag atttatttcc attctgctaa
     3361 gactagttga tgatagtgac gtaaaggtcc gaccttttgg aggaggtagg tagcagccag
     3421 tccgaccaat aagcctgata aaattcctgt aaaagcgagg attggcaggt agtttagtac
     3481 atagaaagaa cgggcaatgg tagcaaaaac aagaagctga ccaagattgt gcaacatccc
     3541 gcccagaata gaaattccaa taggactgac acggtttgga ccgagctgct tggcagagag
     3601 cattcctaca taactcaaag tagttccggc aaagccatag aggaatgtgg aaaaggtgcc
     3661 tcccagaaag gttgagatga gcaagcgtag agcgacaacc tttaggctat cctttgtagg
     3721 gagggtaaag atagcaatta agctaatcaa atttcccagt cctagcttgg cgcctggagc
     3781 aaaagcaaat gggggggtga tgagtcgttc aatcaaagaa atgacaactg cctgagctgc
     3841 tagcatcgta ataaagttaa gtgtttgacg tttagaattc atgggtgtag agaaaaaact
     3901 ctttctagtc gattatctat tttactatca tacttgtttt tgttcactat ggcaagtttt
     3961 gtttgttgta tattactaat tattgaaatt cgcttgtcaa ctatcttgga atacgatata
     4021 atggaaacat gtaatttgtt ttgattttca caaggaggta aaattgtgac gaaaaatatt
     4081 gtgattgtcg gtgctggtta tgcggggatt gcagcagctc gattgctagg aaaaaccttt
     4141 aaaaaggatc aagatgtaac agttaccttg attgataaga attctttcca tacctatatg
     4201 acagagttgc atgaagttgc ggctgggcgt gtagaagcca atgcaatcaa gtatgacctt
     4261 caacgtatct tcaaaaaata tccaaaggtt caactggtaa cagacaaggt tgttgagatt
     4321 gactacgata agaaacaagt agttgcggaa caccaaattc tggattttga ttacctgctc
     4381 cttgcaatgg gtggtgaggc taatgacttt ggtgtgaaag gtgttaaaga acacggtttt
     4441 acactttggt ctatcgaagc agcaaaacgt ttgcatgacc acatgattga tgcttgctat
     4501 cgtgccatgc gtgagcttga tgaagccaaa cgtcgcgctc ttttaacctt tactgttatc
     4561 ggtgctggct tcacaggtat tgagatgatc ggtgagttga ttgactgggt accaattttg
     4621 gcgcgtgagt tcaagttgga tccaaaagaa ttctcactta aggttgttga ggcaacgcca
     4681 aatatcttgg ctatggttac tgaaaaagag caagtcaagg ctcgcaaata ccttgagaaa
     4741 aaaggtgttg aattggttct tggcgatggt gtagcaagtg tacaagaaga tagcttgaca
     4801 ctttcatcag gtcgtcaaat tcctacctat acctctattt ggacagctgg tgttcaagcc
     4861 aatacagacg caagtgaatt tggtattgaa aaagcacgtg caggtcgttt ggtagccaat
     4921 gagttcatgg aagcgaaagg caaggaaaat gtctatgtag ctggcgactt ggtttacttt
     4981 gaagaatcag aaggaaaacc aactccacag attgtacaag cagctgaaca gacaggccat
     5041 actgcggcaa gtaatattat tgcagcaatc aagggtggcg aaaaacatag ctacaaaggt
     5101 aaatatgatg ggtttatggt atctattgga gcacgctacg gtgtagcctt cttgatggac
     5161 aaataccaca tgtcaggctt tatggcaatg gctgtcaaac acatggtaaa cctactctac
     5221 ttctttacta tccgtagttt cttctacatg ggttgttatg ttcgtcatga attctttgac
     5281 attcagaata agcgaaacat ttttggcggt catacttcag gcaaaggaaa tcttctctgg
     5341 tctgtgccaa tgcgtgtcct ttatggttca gtatggctct acgaaggtat caagaaagcc
     5401 tttggtctat ttggaacaac atcatggttt ggggatcaag ttgtcttccc attcccttgg
     5461 ttagctgatc cagtatctgg agcatctgct gctgaggcgg tttcaagtgc ctcacaagcg
     5521 gcaactgctg cggcagaagt agctgaacca atttttggtt tgagctatgc ttatggtgaa
     5581 gcaccgatgg ctgttttgga caaaatgcct gattggtttg ctactatcat ggaatttatg
     5641 atgccaaacc aagaagtagc actctttatg caaaaattta tgacagtagc agaaatcggt
     5701 attggtctgg ctttgattgc tggtgccttt gtatggattg tatctgctgc aaccgttgct
     5761 ttggtagtta tgttcagcct gtcaggcatg ttctactggg taaatatttg gttcatccca
     5821 gcagctattt ctcttatgaa tggtgcgggt agagccttcg gtctagacta ctggattatg
     5881 ccatggttgg gtcgtttcct agataagaag atttatggaa agccgaagca tatttaccgt
     5941 atcaaggata aaaaataaag gaaatataga aagttagtgg tacatcaaat ttggaatacc
     6001 tatcctgaga ttgagagggg acttgaagag gtaaaatcca tcatcttatc tgagatgacg
     6061 gtcctgcatc cagcagttac atcgaagatt gttgaatatg tagaggcacc agggaaatac
     6121 ctgcgtgctg gcctctgtct actctttgca caaatgacgg aaggaaagat tagccgttca
     6181 aagctatatt ttgctgccca tttagaagtt ctgcatctgg cgactcttat tcatgacgat
     6241 gtgattgatg gcgcggataa acggcgggga gtcacggcgg ctcatcaaca attttcgaat
     6301 cgaatcgcta tctacacggg tgattatctt ctggcctact caggtcgctt gcttggaaaa
     6361 gggcttcgtt tgctagaagt atcacgtggt gatttggatt tgggcaatga taagctgatg
     6421 gaaacaattc ttcgagggga attatctcag ctactcaatc aatttgatgc caatatgact
     6481 atgaaagcct atctcaagca aattcaaggg aaaacagctt ttctatttgg acttgcttgt
     6541 cagttgggga gttttgtacc aggccaatct aggaaagaca gtagcatggc ttttcggtct
     6601 gggcaagcac ttggcatggc ctttcagcta cgagatgact taattgacta ccagctggat
     6661 gtggcagaat caggaaaacc tagattacaa gatattcaaa acggtatcta tactgcaccg
     6721 ctactatttg ctatgcgaga agacaagtct atccagcaac aattgcgggc ttatattcaa
     6781 tcccctacgg cagaagggtt ggaaatcctt atagatagga tttttgcaac gcatgctatt
     6841 gccaaaacag agaaactgat tgttagctat ttggaaaaaa tgatgagttt tgcagataga
     6901 ataccacagt tatcgactgc gtctatgcaa aatttggtgg caatagtttt tagtcattat
     6961 ttttaaaaaa taactagaca ttttctctaa ttaggtatat cattataagt ataaaactat
     7021 ttacttaaca aaaggagcat tgttatgaaa acaactaaag ttgtgttgaa aagtttggta
     7081 gttcttggtg cggtatttgc acttgctgct tgcggttcta aacaagaatc tacttcaact
     7141 tcatcttcaa ctacagaaac agtagtattg aaagatggta cttacaaagc tgagtcaggc
     7201 aaagacgatt atggctacaa aattgttcac acattgactg ttaaagacgg aaaaatcagt
     7261 gagtcaaaat ttgactacga agctgaagat ggaagtctta aatcagcaaa tgaagagtac
     7321 aacaaaaaca tgaaagataa agctggagtt tctgctggtg aagcaatcga acaactcaat
     7381 gcagctttgg ttgagaaaca agacctctca gctgttgaag ttgtatcagg tgcaactcat
     7441 acttctgaag acttcaagaa gagtacagaa gcccttcttg cagcagcagc tgaaggaaaa
     7501 acagaaactg taaaacttga ctaattaagt ctatacagaa aagacacagt cgcacttgtt
     7561 tggctactgt gtcttcttat ttttggggga aggtaaaatc agtgaaaaaa attgcaagtc
     7621 tattgttcgt gggcctactg atggttttgc ttgttgcctg tggtggtaaa caaaacgaag
     7681 gtttatctgt tgtgaaaact ccgttgactc gtagtgaaag cttacttcac acagtagtgc
     7741 agttgagtat ttatcatgaa aatcaggaag cagcgatgga tgaagctgtc tcctatatca
     7801 aggagatgga aagtttgttg tccacaaact tggaaggggc agatgtctac cgtatcaatc
     7861 aggcagcagg aaaagaagct gtcaaggttg atgaacgtac ctttgaagtg attgaaacag
     7921 ctattgacat gagtaaagaa agtcaggggc tatttgacat ttccattgga gctgtgagca
     7981 atctgtggaa aattggagat gaggatgcac gtaagccgag tgatgaagaa atcaaagctg
     8041 ctctgccatt tatcaattat aaagacatta ctttagataa ggagaagaag actgtcttta
     8101 tcaaagaagg aatgacctta gaattaggag ccatttccaa aggttacatt gcagataagg
     8161 ttagggaatt gtttgccagc aaagggatta cgactgctat catcaatcta ggtgggaatg
     8221 tagtggttat gggggattcg ccaacgacac aaaatggttg gaatgttgga gttcaagacc
     8281 cagatcaagt gcgtggatca acggtcggtt ctgtgccagg gacacatgat tcggttgtta
     8341 cctcaggtat ttatgagcgt tacttagagg tggacggtgt caaatatcac catatccttg
     8401 atcccaaaac tggatatcct gtggaaaacg atatttcagg tgtgacagtc ttttccaaaa
     8461 catctatcca aggagatgcc ttctccacaa ctctcttctt actaggtgtg gaaaaaggtc
     8521 ttgactttat caatcagatt gacggtgtag aagctgtttt cattgataag gagcagggcg
     8581 ttcatctgag tgatggcttg agagaaagct ttgaattaac gaatgaggag tatcatcttg
     8641 tcaatgaata aatcaactaa agggctcagt ttgcctgtat ttctagaatt tgttgaactt
     8701 aaaactaaag ttgccagtgt ttttccgatg accctgggga ttttatggag cctgtatcgc
     8761 tatcaagctt tcaattggct caatactctc ctcttcatta ttgccgtcct tagctttgac
     8821 atgtgtacaa ctgccatcaa caatagcatg gactaccaca aggccaagga tgaaacctat
     8881 cgtcaggaaa gcaatgtgat tggaaaattc tccctagatt tccgtcagat gattgggatt
     8941 gtccttgccc tacttatttt ttctctactg gtttcattac tccttgtatg gcggaccagc
     9001 tggctcctat taccaatggg ggctctctgt ttcctcattg gtatcttcta tacctttgga
     9061 cctattccgc tttcgagaat gccactgggt gaagtctttt ctggcgtaac catgggattt
     9121 ggcatcttct ttcttgctgt atttattcag caaccagatt tgctactgac cagccaagtg
     9181 agcggccagt ggatgacctt gcaattctct tggacaaaaa taatagaaat tattttcatg
     9241 tccattcctc tggtaacctt gattgccaat atcatgttgg ccaacaatac ctgtgattta
     9301 gaagaagata ttcgcaacca tcgctatact ctggtttact atattggcaa gaaaaatgct
     9361 cttaaattgt attttgtctt ggcaagttta ccatggttgc tgtggataat ctactgtctg
     9421 ataggattct taccaatttg ggcactggcc ggtttaatcg gagtttggcc tgcttataag
     9481 agtctagaaa catttttgaa aaagcaagtc aaacgggaaa cctttatcga ggcagtaaag
     9541 agttttgtac tctttgcact gctatatgtg gtgatacttg ctttagccct tattttctaa
     9601 aaaggaaaga cacagtcgtt catcaggctg tgtcttcttt gttatatcgt catttagttt
     9661 ttcttttatt acttcgacga gcggggagcg ggaaagaact cgactggtca aaaaaagagt
     9721 tcgtcttccc gcccccgcac agttgattag gtcagatttg gagtgtaaaa cacgaacaaa
     9781 cctgccaatc aaccactgcg ctgagatgtt gacacgaacc ctgagaagcg aggttgggct
     9841 ttttgcccag cctcaatttc caacttcaaa cactccactg gagtatttga actcgccttg
     9901 cctgattgaa acagtctggg ggactgtttc aagtcaatgt ctagaaataa gaaagcattg
     9961 agttatgcct gcggctcgtt tcctagtact aaaagtaaac taaaagacta tagtggtaaa
    10021 atgagttctt cttcatcagt gctttctccg ccaacggatt tgatttcaat caacaggtta
    10081 tgtggtaggc agacggagag ttgacccggc tggctaatcc agctggtcat aacggcaatt
    10141 tggtctgggc tattgtcttc cttgacacga atgcgttcgc catcaacttc aatgatattg
    10201 tattgccctt cattgggata gtaggtcttt tcttgatggg gtgtgtcctt tgataattca
    10261 aattggtcga ccacctcacc attgatgcgg acataggcaa ttgtttttgc ctcattggca
    10321 tctgtagtca gatgtgtata ggtaaaaatt gctggaagaa aggataaaac tagggtgaat
    10381 ccgattaaga tataatcaaa tagtttgagt tgtttgagaa tggatttgaa tgtcatatac
    10441 aatctatttt atagaaaaaa tcctactttg taaaatgttg gatacagatt actttttcaa
    10501 ttgtctaaag aagagtagga tggctaacat tccccaaaat ccagcatgaa gtggctgaat
    10561 ttttccagcg aaaaatacaa tggagaaagt tagggcaaaa cctgcaaaga gaaaccagag
    10621 cagtggttta tgataaagat agatagcttt ttggaaagca tttgcttctg ggccactagt
    10681 ctgccagtca ctattcaaga ttggaagtat agtgaacagt aagataaaaa ttggtggatt
    10741 gtccccagtc aggtagctat gtatatacag gctacaaatt gctgtatagg agagaacaat
    10801 caaagtgagt ataagtttcg gtttgttaac gttcatctgt tttctccttt ttgaaataca
    10861 atcgataaag aatagcgaca gcaaatccag caggaaggat gaggatatgt ggtttgacat
    10921 gtcctagcca aatgcccacg gaataggtca aggtcatggc aaacagggca aagatggctt
    10981 gaatgataaa gtatcgtttt tcttcggctg tttgatagga tttgatgatt tgaatggtcg
    11041 gtaaggtaag caagaatgaa ccgctaatcg gctcattgac catgtaagag tagatgaaga
    11101 aggcagaaaa tatagcataa aatagaacaa aaaatcgaca ggtttgtttg ggtgtaaaag
    11161 gtttattcat ggtgttaggt ccttattttt tcttagtagt ttctgcttcc agtttatgca
    11221 gataggcaaa ggcagacagg gcaatgctag caaagatacc catcgtgcca ccgaatagga
    11281 gggattttgt atgcaagatt gaggccgtta ggggaagatt gaggctaaag aggtctgcta
    11341 ctccagcaaa aattgtcgaa agcagtgcaa ctcccagtcc ccaaagaatt gggttaagag
    11401 gccgaccttg tctttcctca gggctaagct catccaagtc atattggggc aagtcagcat
    11461 ggagggtctg catattgacg acacctagca agataaagct aaagagagca atgatcattg
    11521 gataatagtt ggcaacctcc acaggatagc ggatagctag gaagtaggcg atggcatttc
    11581 caagagctag gaagtagaag tggtagataa aggctattcc cccaatctca tcggccttag
    11641 tccgtcggta ctcgtctaaa ggaccacgga taccatagaa gtgtcgtatc atatttttga
    11701 tccacttagg ttcttctttg gcaatagcag agctgacact tagttgttcc gcttttttca
    11761 tgcgcttgcg taacatgatt tcgatagcaa tgcctgtgaa aactccacct agacaggctg
    11821 ctagaaattt tcggaggtca aataggtggt cgagaggatt gagtccctcc atccaggcct
    11881 caaaaacaac agtccaaatg tacataacgg tagtgaaata gagggcgaat ttgatacttg
    11941 ctcccttgag ttgtttctgt tccttggtag tcagttcttc tacatctaaa ctgtccagtt
    12001 gactgtgccg gagtttaaac attatgtagg tactagcaga taggtaggct aggagcagag
    12061 taacggggta gccataggca acgacttctg ggtagcgttt ggatagagcg agtgccagta
    12121 gggtaagagc catggtgccc caaaagagca ggataaagca ggtattgcct agtcggaagg
    12181 cttctttttg gcgaaattca tccaagggcc ctgcgatacc gtaaaatcgt ttgattaatt
    12241 tatcttgaaa ggtttctttt ttcataagca gctccttatt tttgatcgaa caatggtaag
    12301 atgtaaacaa agcctaagaa gtagatggtc ccagcaatca gcacttgtag ccaaaatttt
    12361 gcttggctga tggtgtgcaa gatttctgct ccgctattgc cccaaagctg aaagaggaca
    12421 aagagcggga taaaaataag ggtagagata agagcttgac gtatattctt tttcatggtt
    12481 tttcttcctt ttatttgttt gcgagaattt tcttttgtaa ctgcttctta caatagtagt
    12541 aatggatgaa ggtgatagct cctgcaacaa ttagccgacc tagtaggagg atcgggtctg
    12601 ttagttcttc aagaaataca gaccattctt tatagaaagc aggggcagtc gtggaagcca
    12661 ggaaggctcc gaaaaatgtg ctacgactac tttttacaag agatttattg agcttggtca
    12721 catcaatttc atcctcatag ctttgactaa tgccattttt caaggcaagg tgggaagcta
    12781 aggcttgcgt gaaaccgatg attagtatat tggttagggg gagcagaaag ccaacttttt
    12841 caggttgtcg taaggcgata agaagtgcaa tcgtatttcc tattaccagg ataggaaaaa
    12901 caatcatgac aatggtgtta ctgaggcgat agctttcttg ccgccgcatt tcatccatgg
    12961 gaccactaat ggaataaaaa tccttggttg cttgattgag caattttgta actaaatctt
    13021 tcatgaaaaa ctccttattc attccaaaag agactattga gatcggtctg taaagctttg
    13081 gctaggttga tacaaaggtc tagcgagggg ttgtacttgt tgttttcaat catgttgatg
    13141 gtctgcctgg atacgctgat ggccttggcc agatccagct gagaaatacc tagcagtagg
    13201 cgataatctt tgacacggtt catgtgccct cctttctatg tcaattatat ctgacacaat
    13261 tgtctaaaaa tgagaggata ggttgactat ccttttaaat tgttgtcaat tatatccgac
    13321 atacaagtgt aaaaaaaatg tgggagacct ccctgttgat gtcaactata tgtgacatgt
    13381 ttctttactg ttagtatata cctgttcata taagttgtca actatatttg acagtttttt
    13441 gtaaaaagtt tgcaacaact ttttatttta atttgtggta caatagaaaa gatatgacca
    13501 aggaatttaa tcacacaact gttctcttgc atgagacagt cgatatgtta gacatcaaac
    13561 caaatggaat ttatgtagat gcaacgctgg gtggagcggg tcatagtgag tacctgctca
    13621 gtcagttgac ggatggtggg catctttatg cctttgatca ggatcagact gctattgatc
    13681 atgcccatat tcgcttggca tcctacattg aaaagggaca agtgaccttt atcagggata
    13741 atttccgcaa tctcaagact cgcttggcgg agctaggtgt aacagagatt gatgggattt
    13801 gttatgattt aggagtatct agtccacagt tggatgagcg ggagcgtggc ttttcttaca
    13861 aacaagatgc tccgcttgat atgcggatga atcgtgaggg acatttgacg gcttatgatg
    13921 tggtcaataa ctatgactat cacgacttgg tacggatttt ctttaaatac ggtgaggata
    13981 aattttccaa gcagattgct cgtaagattg aacaggtgcg tgcggtcaag ccgattgaaa
    14041 caacgactga gttggcagag ctgatcaaat ctgccaagcc agccaaggaa cttaagaaga
    14101 aggggcatcc tgctaagcag attttccaag cgattcggat tgaggtcaac gatgagttgg
    14161 gagcagcaga cgaatctatc cagcaggcca ttgacttatt ggctttggat ggacggattt
    14221 ctgttattac tttccattct ttagaagatc gtgtgaccaa acaactcttc aaagaagcaa
    14281 gtacgattga tgttccaaaa ggtttgccat ttattccaga tgacttgaag gcgccacttg
    14341 aattggtcaa tcgcaagccg attttgccaa gtcaagaaga attagaggca aataatcgcg
    14401 cgcattcagc caaactgcgt gtagctaaga aagtacataa ataggagaga gtctatgttg
    14461 caagaaaaac gtagagaagc cactatgcgg gttatcggtg ataaaattag aaccttcaca
    14521 cgtgtagaaa aagccttcta ttcaagcatc gtattatctg gattagccct tgcgatagga
    14581 attatcttta tgcaaacgcg gcttttgcaa cttcaatctg gcatggccaa agtcaatcaa
    14641 gatattagcc aaaaacagat tgaaattaac gatgcaaagc aagctgtcaa tgaattgaca
    14701 cgttcagctc gcttgatgga aattgctgaa aaagctggat taacttttaa taatgacaat
    14761 attggagtag ccgaataatg cccagaagaa ataataaact cttgcggtat gtcttgaaaa
    14821 aacgatatat tccgtcaaaa aatcgtcgaa gagtaggaca gagcctactt cttttgacgg
    14881 ttttcgtctt ttttctcttt ttaatcaact ttgcctatat tatcggcacc gatagtaaat
    14941 ttggggtgga cttgtcagag agagctgacg cggtacacca gcgtgaagtt accgttcaag
    15001 ctaaacgggg gacgatatac gaccgtacag gaattcctat tgcagaagat tcaacgacct
    15061 atacagtcta tgcaatcatt gacacagaat atgtgtcaga actgaaaaaa gttttgtatg
    15121 tgcagtcttc tcagttcagc aaagtagcag aaatctttaa aaagtatctc ggaatggaga
    15181 cagattatgt tatggagcag ctgaatcaac cgaaactgaa gcaggtatac tttgggacct
    15241 taggtaagaa tatcccctat agtacaatgt cgtcaatcca agaagagatg aaggcggcag
    15301 gtattgaagg gattgccttt gacgctagtc caggacggat gtatccgaat ggaaattttg
    15361 cttcaatttt tgtcgggtta gctaatccgg ttgaaaacaa ggatggtagt tatagcttac
    15421 agggtgtgag tggtttggaa tattatctga atgatattct ggctggtcaa gatggaaagg
    15481 ttatctatga gaaagatagc caaggccgtg tcctacctgg gacagaaaag gttgaaacgg
    15541 agacagtaaa cgggcaggat gtctatacga caatttcagc cgatctgcaa cgttctcttg
    15601 aaagcaatgt gaataccttc tttgataacg ccaaagggcg atatgcgaat gcaactttgg
    15661 tttcagccaa gacgggtgaa atactggcga cgacccaaag accaagctat gattcagata
    15721 ccaaagaagg tctgggacaa gaaggactgc tagagcggtc actcttatat caagaggcct
    15781 ttgagcctgg ttcaacaatg aaggtgctaa cagtagcgtc agctattgat aatggaacct
    15841 ttgatccaaa tgcatattat accaataatg agtacgttat tgcagacgcg aaaatcaatg
    15901 actggaccct caatgcaggt tattcagctg tgactctcaa ttatgcgcaa ggcttctccc
    15961 tatccagtaa cgttgggatg accttattgc agcaacagat gggagatgaa aaatggctaa
    16021 attacctcgc taaattccgc tttggctacc caacgcgttt tggtatggga gacgaggcac
    16081 caggaatggt accagaggat aacattgttt ctatcgctat gtcatcgttt ggtcagggta
    16141 tttctgcaaa ccaagcacag atgcttcgta gttttaccgc cattgcgaac aatggtatta
    16201 tgctagagcc taaattcatc tcagctcttt atgatcccaa tacagataca gctagagttt
    16261 cttcgaaaga agaagtagga aatccagttt ctgaacaagc tgccgacgat acacttcgct
    16321 atatgattaa tgtcggtact gaccctgttt atggcacact ctactcacat gacttacagg
    16381 ggcctgcaat tcaggttgac ggctatgata ttgcagttaa atcaggtacg gcggaaattg
    16441 ccagcgaaga tggtcaaggg tatattaaag gtgcctacat caactctgta gtagccatga
    16501 tccctgctga ggaccctgaa tttatcatgt atgtgaccat tcgccaaccg gaagttttaa
    16561 atgtcctgtc ttggcgtgac attgtcagtc ctacattgaa agaagcgatg ctgttgaaag
    16621 acaatttgaa tctggattca cctgcaccag cattggcaca ggtcgttaat gagacagagt
    16681 atcaattgcc agacctcatt ggtaaggcac caggcgagtc ggctgaggaa cttcgccgtc
    16741 aattagttca accagttatc cttggaaacg gtgctgaaat taaaaaagtt tcagtcgaaa
    16801 aaggaagtaa cgtaatcgcc aaccaacagg ttctactctt gacaaataaa ttcaaagaaa
    16861 tgccagacct gtacgcaatc accaaagaga atatggatat atttgctgaa tggactggta
    16921 tcgaagtggc ctataaagga acgggttcaa aagtggtcga ccaaagtgta gaggtcggta
    16981 ccgctttgaa taaaatcaag aaaataacaa taaccttagg agactaacat gcaattcgca
    17041 cttatgtcgg gattggtagc ttttctagca accgttctct taatcccacg atttattaca
    17101 ttttatcaag ccaaacgtat cgaggggcaa cagatgcacg aagatgtgaa acaacatcag
    17161 tttaaggcag gtacaccaac aatgggggga acagtttttc tcgttgttgc gatacttgtc
    17221 agcttgttgt ttgcaacggc ctttcaactg ttgacgggtg gtgtccttgc tatcctcttc
    17281 atccttgccc tttatggtgt agtaggattt ttggatgact ttttaaaaat tttccgtaaa
    17341 ataaatgaag gtcttaatcc taaacaaaag cttgctctac aaattttagg aggtattgtc
    17401 ttctactttg tccacgtaag aggagcaggt ggcggagaat tgaatgtatt tggtcacatg
    17461 gtacatctgg gagttctcta tttcccattt gttctgttct ggctggtcgg tttttcaaat
    17521 gcagttaatt tgacagatgg tattgatgga ttggcttcta tctcagttgt tatcagcctt
    17581 ctagcttatt ctgtcatcgc cttcaatgag caaaaattcg atattcttct agtttgtgtg
    17641 actatgattg gtggcttgct cggtttcttt gtttataacc gtaaaccagc gaagattttc
    17701 atgggggatg tgggaagctt ggctctcggc ggtatgttgg caactatttc aattgccctc
    17761 cgtcaggaat ggacactcct ccttatcggc ctggtctatg tcattgaaac gtcctctgtt
    17821 atgttgcagg tttcatattt taaatacacc aaaaaacgct ttggagaagg tcgtcgtatc
    17881 ttccgtatga ctccttttca tcaccattta gagctcggag gtttgaccgg aaaagctgaa
    17941 aaatggagcg agtggaaagt tgattttttc ctctggtcag tcggtctcat catgagcttg
    18001 attaccctag ctattcttta tctataaaat gtcagaaaca gcagaaatgc tgttttttac
    18061 cttgacaaat atggtcgggg gaatagagag aattggtgga ggaaaaaaat ggaacaattc
    18121 aatcaagaac agttatatgg agtggttatt caaaccatga cagcttctag tcaacttcct
    18181 atggttaaag tcaatcggga agaatttttg aagactcgtt ttaaagatag tccctatcta
    18241 gataaaatta ttgcggatgg accaacggcg gtcttcacta caaaagcttt gcgtaaggaa
    18301 gctgaaaaaa tcattaatga tatgactacg aaaacatctt tgatttcttt cgcagctggt
    18361 ttaccgggga atccatttac agcggtggcg gcaggtactg ctgatatagc tcagtatttt
    18421 ggttttgcct tgaatttgtc gcaacaaatc gcttatcttt ttggtgaaga tgaattattt
    18481 gttggtgaag ggaaagaaat ttcataagag gttcaaatcc gcattattgc ctacattggt
    18541 gtcatgtttg gagcaggtgg ttcagcagtt cttctcaata aagtatccaa gacagtcgga
    18601 gctaatatcg gtaaaaagta gctatgcagg ctttgaccaa gacaacttgg tatcccctgg
    18661 tgaaaaaggt aaccgcagtg attggtgtga aaattaccaa aaagactgtt gaaaagacta
    18721 ttgccaatgc agttcctatt ttgggtggcc tgatttcagg aggtttgacc tattttacct
    18781 tccagcccat gggagggatg ttggcagaca catttgtgaa aaaaataaat ggtgattttg
    18841 atgccggatt agaactcact gaggccttta aagcaagttt ggaagaagag aaggaacttg
    18901 ggattattga ggcagaattt actgaagcaa gcgagagttg attaggacgg gtatccgtcc
    18961 tttttgcttg tccacctaaa tcgtgctata atagagggaa atacaactaa agaggaaact
    19021 atgaaattta cagatatgaa actcaagcct tatattcagg aggctttgaa agaaattaac
    19081 tttgtccagc cgacagaagt tcaggagaag ttgattcctg ttgttttgtc agggcgagac
    19141 ttgatagggg aatcaaaaac aggttcgggt aagacccata ccttcttgat cccgattttt
    19201 caaaaattaa atgaggaatt ggaccaagtt caggctgtta ttacagcccc atctcgtgaa
    19261 ctggctactc agatttatca agcggcacgc cagttggcta gtcattcgga catcgaagtt
    19321 cgtgtgacca attatgttgg cggtacggat aagaaccgtc aaattgacaa gcttcaagct
    19381 agtcagcctc acattgttgt tgggacacca gggcgtatct atgatttggt caaatcaggt
    19441 gatcttgcta tccataaagc caaaaccttt gtggtggatg aggcagatat gaccttggac
    19501 atgggctttt tggaaacagt tgataagatt gctggcagcc tgccaaaaga cttgcaattt
    19561 atggtctttt cagcgaccat tccgcagaaa ctccaaccgt ttttgaaaaa atacttgtcc
    19621 aatccagtca tggaacaaat caagacccat gcggtcattt cagataccat tgaaaactgg
    19681 ctgatttcaa ccaaggggcg tgaccgcaat gctcaaatct tggaagtaac caagctcatg
    19741 caaccttact tggccatgat ttttgtcaat acaaagacac gtgcagatga attgcatagc
    19801 tatttgactg cgaatggtct tcgtgtcgca aagattcatg gggatattcc tccacgtgag
    19861 cgcaagcgaa tgatgaacct ggtgaagaac ttggactatc agtacattgt tgcaacggat
    19921 ttagcagcac gtgggattga tattgagggc gtcagtcatg tcatcaatga tgccattcca
    19981 caggatcttt ccttctttgt ccaccgtgtg ggacgcacag gtcgtaatgg tttatcaggc
    20041 attgccatta ccctttatca gccaagcgat gatgcagata ttcgagagct agaaaaactc
    20101 aatatcaaat tcttgccaaa agaaatgaaa aatggcgaat ttgtcgatac ctacgaccgt
    20161 gaccgccgtg ctaaccgtga aaaatctcgt gaaaaactag atttggaaat gattggcttg
    20221 gtcaagaaga aaaagaaaaa agtcaaacca ggctacaaga agaaaatcca atgggctgtt
    20281 gatgaaaaac gtcgcaagac caagcgagtt gaagcgcgtg caaagggtcg tgcagaacgt
    20341 aaagccaagc gtcaaacctt ttaaagtaat cgaggaaaaa gatgttacaa aacattctaa
    20401 gtttttattt acaaggagtg ctaatagctt ccttgttgat tatcacttct agtctggtct
    20461 ggtttatctg gcgtgcgaca aagggtgtgg ataaaacctt gcaagaacga caagatttct
    20521 tgtttgattt gttgatgatt aatgtgatga caattcccat tgcagccttt ggagtagtgg
    20581 gaatcttatt aatgtttaaa gcgtagaagg aaaaagtata tgtacgatac agttgtaatt
    20641 ggtgcgggtc ccgcggggat gactgcagct ctttatgcag ggcgtagcaa tttaaaggta
    20701 gctcttttgg aacgtggtat ttatggtggt cagatgaata ataccgcgga aattgaaaac
    20761 tatccaggct atgaccatat ttcaggtcca gccttggctg aaaaaatgtt tgaacccttg
    20821 gaaaaattcg gtgtggacca tatttttggc accctagtcc gtattgagga agaaggacaa
    20881 attaagaaag tgattactga ggatggtgtt ttggagacaa agacagtcgt tctagccatg
    20941 ggggcaaaac atcgcttgtt aggtattcca ggtgaggata cttacaatag ccgtggtgtc
    21001 tcatattgtg cagtctgtga cggtgccttc ttccgtggac aaaaactctt ggttgtcggt
    21061 ggtggagatt cagccgtcga agaagctctc ttcttgacac aatttgcgga gtctgtgaca
    21121 attgttcacc gccgtgatca gcttcgtgct caaaaagtca tccaggaccg cgcctttgcc
    21181 aatgaaaaaa ttaacttcat ctgggatagt gtagtcgaag aaatcaaggg tgatgactta
    21241 cgtgtacaaa gcgtggttat caaaaatgtg aaaactgaag aagttagtga acttgatttc
    21301 ggaggtgtct ttatctatgt cggtctggat ccgatgactg atacagttgc ggacctagga
    21361 attacagacg aagctggttg ggttatcact aatgaaaaga tggaaacgac tcaatcaggc
    21421 atctatgcca ttggtgatat tcgtcaaaat caacttcgtc agattgcaac ggcagtagga
    21481 aatggtgcag ttgctggtca ggaagtctat aactatatca cagaactagc tgaatagggg
    21541 aatacaatgt ttcatattta cgatattcag aaaaatccag atggaatctc ctttgataaa
    21601 accttggatt tacaggaaga attgcaagct cgaaatggtg aagttctagg cttgtcgcca
    21661 gtccaagtca ctggaaatat ccgttttgaa tcaggtttct ttttcttgga ctatcagatg
    21721 acttatgata ttaccttggc atccagtcgt tctctgcaac cagttgtttt gcatgaggtc
    21781 caaaacgtta atgaactctt tgttgccaat gaagcagttc ttaaagaaca agatttgatt
    21841 gatgaggata tggtactggt agtagaagat gaccacattg tccttgatga gagtgtagca
    21901 gataatattt tactggctat accaatcaaa gtcttgacac ctgaagaaga agctagacag
    21961 gaactaccat ctggtaaagc ttgggcttta atgactgaag aagattttca acaaaaagct
    22021 caagagaaaa aagaggccaa tagtcctttt gcccaattac aagggttatt tggagatgag
    22081 gactaactca gattggcaag atagttaaat tatctgaaaa ttcttgtcaa ttttaagctt
    22141 tcggtgtata ataaaagaga tacaaactag aggagtaaac aaatgactaa agcaaatttt
    22201 ggtgttgtag ggatggccgt tatgggccgc aaccttgcgc ttaacgttga atcacgtggc
    22261 tattcagtag ctatttacaa ccgctctgct gataaaacag aagacgttat tgcaagcaac
    22321 ccaggtaaaa acttggtacc aagttacgat gtggaaagct ttgtggcatc tatcgaaaaa
    22381 ccacgtcgca tcatgcttat ggttcaagct ggtcctggta cagatgcaac cattcaagcg
    22441 ctcttgccac acttggatga aggtgacatc ttgatcgatg gtggtaatac tttctacgaa
    22501 gacactatcc gtcgttcaaa agaattggca aactcaggta ttaactttat cggtacaggt
    22561 gtatctggtg gtgaaaaagg agcccttgaa ggtccatcta tcatgcctgg tggtcaaaaa
    22621 gaagcttacg aattggtggc agatgtcttg gaagaaattt cagcaaaagc accagaagat
    22681 ggagcaccat gtgtgactta tatcggtcca gatggagctg gtcactacgt aaaaatggta
    22741 cacaacggta tcgagtatgg cgacatgcaa ttgatttcgg agtcttatga cctcatgcaa
    22801 cacttactcg gtttgtcagt ggatgaaatg gctgacatct ttactgagtg gaacaaaggc
    22861 gaattggata gctacttgat tgaaatcaca gcagatattt tgaaacgtaa agacgatcaa
    22921 ggtcaagatg gaccaatcgt tgattacatt ttggatgcgg ctggtaacaa gggaactggt
    22981 aagtggacaa gccaatcatc acttgacttg ggtgtgccac tgtccttgat tacagaatct
    23041 gtatttgctc gttatatctc aacctacaaa gatgagcgtg tggcagcaag caaggttctt
    23101 ccaaaaccaa ctccgtttgc atacgaaggc gacaaggctg aattggtaga aaaaatccgt
    23161 caagcattgt acttctcaaa aatcatgtct tatgcacaag gttttgcaca gttgcgcgta
    23221 gcatctaaag aaaataactg gaacttgcca tttggtgaaa tcgcaaaaat ctggcgtgca
    23281 ggttgtatca tccgcgctcg cttcttgcaa aaaatcacag atgcctacgg acgtgatgaa
    23341 gacttggcaa acttgctctt ggatgagtac ttccttgatg taacagctaa gtatcaacag
    23401 gcagttcgtg atgttgttgc cttggcagtt caagcaggag ttcctgtacc aacattctca
    23461 gcagctatta cctacttcga tagctatcgt gctgaaaact tgccggcgaa cttgattcaa
    23521 gcacaacgtg actactttgg tgctcacact tataaccgta aagataaaga aggaatcttc
    23581 cattacgact ggtatagcga atcaaaatag aaaagatggg cgtggaagcg cccttctttc
    23641 tagtcataat acgattccta caccatttga aagaatagaa aaggatggac aatggctaag
    23701 aaaattttga ttgctggtaa agaacgcaat ctctcgcatt ttgtttccat ggaattgcag
    23761 aaaaaagact atcttgttga ttatgcatcg acagggaaag aggctatgtc cttggcacat
    23821 gaaacggatt ttgacttgat tctgatgagt tttcagcttt cagacatgtc cagcaaggaa
    23881 ttggctacag aactattagc gataaaacca gccacggtca tgattgtggt agttgagccg
    23941 acagaagtta gtcaatatgg ggaagaagtc ctctcttatg ctgtttccta cgtggtgaag
    24001 ccctttgtca ttagtgattt agtggagcaa atctctgcga tttttcgtgg tcgtgacttt
    24061 attgacaata actgtaaaca ggttcacatg catgcggcct atcgtgattt gaaggttgat
    24121 tttcaaaatc gaacagtgac tcgaggtgat gaattgatta atttgacccg ccgagagtat
    24181 gatctgttgg cgaccttaat gaatagccca gagcctgtta gtcgagaaca acttcttgag
    24241 cgtgtttgga aatatgaagc ggcttctgag accaatgtcg tcgatgtata tattcgctat
    24301 ctacgtggta aactggattt gccgcatcaa gattcatata taaaaacagt tcgtggtgtc
    24361 ggatatgcca tgcgcgattg atttagagta ggtattggct tgttttcaag aatttctttt
    24421 ggatagtgat aaagtgaggg ggtagttttt cttcccaaac taaagaaaag ctgcgagtag
    24481 atcatgtgta ccgaggagca gaaaaaatag tcactaagag ccgcttgcga cactgtctat
    24541 caaatatctt caagataccc tttatatctc cgttccaagc gtacgttagt aacttcacac
    24601 atgtcagcga ttagctggta tgtatgacat taagaaggta gaagacatca atgtcagctt
    24661 ccgatgaact tggttgatgg ttactttttg cttgaccttt ttaaaatttg agcgtatatt
    24721 gtatttgtgg aaagggaatt aatactcttc gaaaatcaaa attatccgtt gtcaacttgc
    24781 cttgatgaac tccagttcat gaactccagt tctatcttcg gcttcgtttc ctaggctact
    24841 tttgattttc attgagtata agccaatgaa aaatcataaa attattagtt ttgactctgt
    24901 ttcgttcata ttgggaggaa tattttttgt tctctactta gctttatttg aaagtaattt
    24961 ttataaattt cctaatattc ttttgactat ttattttttg ctttgtctaa tttcttttgc
    25021 ctttatttta gattattata taaaaaaagt accacagtat tcttttgtat taaggctttt
    25081 ttatgttggt ctctatatat tatcccctat acttataata tttcttattt ggagaagtag
    25141 acataaaaaa taaatttatt atccttttga tgttatgtat agttatagtc aactgaagca
    25201 ggaacaggac
//
LOCUS       DATUCG010000016        25210 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.016, whole genome shotgun sequence.
ACCESSION   DATUCG010000016 DATUCG010000000
VERSION     DATUCG010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25210
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.016"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            326..646
                     /locus_tag="VCA20_000541"
                     /pseudo
     CDS             326..646
                     /locus_tag="VCA20_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935801.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(694..2232)
                     /locus_tag="VCA20_000542"
     CDS             complement(694..2232)
                     /locus_tag="VCA20_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HEP1834397.1"
                     /translation="MENHRKEVSFISQSILYSVLRGSLVGIVAGLVVVAFRLAIEKLF
                     SVFTHLYSLATDNAIYLLLIGGLYLVIALLVGKLIKDEPNAKGSGIPQVEVELKGIMD
                     LNWWSVLWRKFIGGVLSIASGLMLGREGPSIQLGAVTAKGVSVLLKSSEMERRSLIAS
                     GAAAGLAAAFNAPIAGLLFVVEEVYHQFSRLVWVSALAASITANFISLHVFGLMPVLH
                     MPKDLQLLSLDQYWIYILLGVFLGLAGYLYEVVILNIQGFYTLLSKIIPLPVPYYSVF
                     AFLFIIPIGYFFPNLLGGGHHLILELPYLEQGLLTLAILILIRFVWSMISYGSGLPGG
                     IFLPILTLGSLLGLFVARFFLDIGFISQEQMLLFIVLGMAGFFAAISKAPLTAIILVT
                     EMVGSLNQLMVIGLVALSSYVMMDLLGGAPVYEAMLEKILPEEVEQQEHMTLIEVVVN
                     ETLDRRFVSELRLPDSCLITSQIHHGKSRIVKGNSQLSMGDSLLVAVPISQIHTVRRI
                     FEGD"
     gene            complement(2346..3416)
                     /locus_tag="VCA20_000543"
     CDS             complement(2346..3416)
                     /locus_tag="VCA20_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEP1834398.1"
                     /translation="MKRLYSFFVGIIAIVLVLWGISYKLEADSTQGTTDKLVIYNWGD
                     YIDPELLAEFTAETGIQVDYQTFDSNESMYTKIKQGGTTYDLTIPSEYMISKMTEENM
                     LIKLDKSKIEGIENIDPRFMGLSFDKNNDYSIPYFWGTLGIVYNETMVENPPQEWEDL
                     WSEEYRDNIMLIDGVREVMGFGLQSLGYSLNSKNPTEIEKAAEHLYNLTPNVKAIVAD
                     EIKGYMIQDAAAIAVSFSGEASEMLDGNENLRYVVPSKGSNLWFDNMVIPKTAKNIDG
                     AYAFMSFMLRPENALRNAEYVGYSTPIPAAKAMLDEETQNDESFYPSEETMKKMEVYD
                     NLGPEILGMYNDLYLQFKMYRK"
     gene            complement(3416..4186)
                     /locus_tag="VCA20_000544"
     CDS             complement(3416..4186)
                     /locus_tag="VCA20_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP1834399.1"
                     /translation="MKKFANIYLTVAFLLLYLPIFYLIFYAFNEGGDMNGFTGFTLEH
                     FSSMLGDSRLMLILAQTFLLAFLSSLIATLIGTFGAIYIYQAKPRFQNTLLSVNNILM
                     VAPDVMIGASFLILFTMIGFQLGFVSVLLSHIAFSIPIVVLMVLPRLKEMNADMISAA
                     YDLGATQPQMLKEIMLPYLTPAIIAGYFMAFTYSLDDFAVTFFVTGNGYSNLSVEIYS
                     RARQGISLEINALSTLVFLFSILLVVGYYFISREKEAN"
     gene            complement(4186..4986)
                     /locus_tag="VCA20_000545"
     CDS             complement(4186..4986)
                     /locus_tag="VCA20_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP1834400.1"
                     /translation="MKKTSRLFAIPYALWIFLFVLAPVALIIFKSFFDIHGNFTLANY
                     QTYFNSPNMTYLRMSFNSIFYAGIITLVTLLVSYPTAYFLTKLKHRQLWLMLIILPTW
                     VNLLLKAYAFIGIFGQHGSINQFLEFIGLGAQQILFTDFSFIAVAAYIEIPFIILPIF
                     NALDDLDKNLINASRDLGATPWQTFTKVIFPLSMNGVRSGVQAVFIPSLSLFMLTRLI
                     GGNRVITLGTAIEQHFLTTQNWGMGSTIGVVLILAMLLIMWMTKERKK"
     gene            complement(4970..6124)
                     /locus_tag="VCA20_000546"
     CDS             complement(4970..6124)
                     /locus_tag="VCA20_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1834401.1"
                     /translation="MKKPIIEFKNVSKVFEDSGTTVLKDISFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLDASSGDIYLDGQRINDVPTNKRDVHTVFQSYALFPHMTVFENVAF
                     PLKLRKVDKAEIERRVTEALQMVRLSGYENRSIQKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTEMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNEGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILPGRMIEDYLVEFNGKRFEAVDGGMRPNEEVEV
                     VIRPEDLQITLPEEGKLQVKVDTQLFRGVHYEIIAYDDIGNEWMIHSTRKAIVGEVIG
                     LSFEPEDIHIMRLNETEEEFDARIEEYVEVEEVEDGLINAIEEERNEENL"
     gene            complement(6163..7071)
                     /gene="murB"
                     /locus_tag="VCA20_000547"
     CDS             complement(6163..7071)
                     /gene="murB"
                     /locus_tag="VCA20_000547"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEP1834402.1"
                     /translation="MKDKIRLELEGIDIRFDEPLKEYTYTKVGGAVDYLAFPRNRYEI
                     VRIVEFAKREGIPWQVLGNSSNIIVRDGGIRGFVIRMDKLNSVTVSGYTIEVEAGANL
                     IETTKVALFHSLSGFEFACGIPGSIGGAVYMNAGAYGGEVAHILVSAQILTPAGYVET
                     LDNRELRFGYRSSILQENGAIVLSAKFALKPGNHTVIQQEMARLTHLRELKQPLEYPS
                     CGSVFKRPLGHFAGQLIMDAGLKGYRIGGVEVSEKHAGFMVNIENGTASDYENLIAHV
                     IEVVEKSSGITLEREVRIIGDPADTL"
     gene            complement(7305..8165)
                     /locus_tag="VCA20_000548"
     CDS             complement(7305..8165)
                     /locus_tag="VCA20_000548"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEP1834403.1"
                     /translation="MKIIIPATSANIGPGFDSVGVALSKYLTIEVFEETDEWMIEHNL
                     EHVPSDKNNLLIKTALKIEKGLQPRRIRMISDIPLARGLGSSSSVIVAGIELANQLAG
                     LNITADEKLLKATEIEGHPDNVAPAIFGNLVISSYVNKRVQAVVTEFPEASFVAFIPN
                     YPLRTVESRGVLPSQMGYKKAVAASAIANVAVASLMAGDLEKAGKAIQSDMFHEPFRQ
                     LLVKEFCPIKQTAQELGAYATYLSGAGPTVMVLAPKDREDAIVLALEELNLDGTVHRL
                     QVDTKGIAIV"
     gene            complement(8169..9455)
                     /locus_tag="VCA20_000549"
     CDS             complement(8169..9455)
                     /locus_tag="VCA20_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019782768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEP1834404.1"
                     /translation="MVVKIGLLGFGTVASGVPFLLEENQEKIEKAAHDSIEVAKVLVK
                     DEAEKDRLLATGHDYHFVTNIDDILADEDIAIVVELMGRIEPAKTFITRALETGKHIV
                     TANKDLLAVHGSELRALAEEKGLALYYEAAVAGGIPILRTLVNSLAADKVTRILGVLN
                     GTSNFMLTKMVDEGWTYEKALQTAQELGYAESDPTNDVEGIDAAYKAVILSQFAFGMT
                     VDFEHVAHKGISNITPEDVAVAQELGYVIKLVGDIQETTSGIAAEVSPTFLPKNHPLA
                     SVNGVMNAVFVESIGIDQSMYNGPGAGQKPTATSVMADIIRIVRRLKDKTIGKAFNEY
                     SRPLQLAAPSDVKSEYYFSIDAPDKNGKMLRLAEIFNSEEISFKQILQQAADGETARL
                     VIVTHEMSKTQLENVTDKLSKETDFTVLNTFKVLGE"
     gene            9560..10483
                     /locus_tag="VCA20_000550"
     CDS             9560..10483
                     /locus_tag="VCA20_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEP1834405.1"
                     /translation="MKHSRRARRSRTQKKQLVPMLLGIALLVVTLLSIFLIFLKQPSK
                     QQSDSKASTVETTTSSSAMTSESSEVQTEIIWEQQTEPIKLPILMYHAIHVMAPEEEA
                     NANLIVDPTTFESHLKALQDAGYYTLSPEEAYKVLTENVLPAGKKVVWLTFDDSLWDF
                     YSYAYPLLKQYQMKATNNVITGFTEYGQAGHLTLDQIKEMRTAGLSFQGHTVNHPDLE
                     YSSIEDQTNELTSSKAYLDSQLNQETIAIAYPGGRYSADTVALTEQAGYKLGVTTNNG
                     LASASDGLLTLNRVRILPTTTAEGLLSEISY"
     gene            complement(10604..12412)
                     /locus_tag="VCA20_000551"
     CDS             complement(10604..12412)
                     /locus_tag="VCA20_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1834406.1"
                     /translation="MKNRSVFTRVWDYLRRYKSSVFLAVFLKTISAVMNALEPFVLGL
                     IITELTKNLLDIANGVEGAQINYSYIAIMLAIYALRALLYEIGAYGSNYFMTKAVQGA
                     TKELRQDLSHKINKIPVSYFDKHQYGDLLGRFTSDVETVSNALQQSFLQLVNAVLTLS
                     LAIFMCFWLDVSLALVVVTLVPVTYFGSKFVMGKSQPYFKQQADALGRLNGFVQENLT
                     GFNVLKLYGREEISTEEFRQITADLQEVGFKASFMSGLLMPLVHGFSNIAYVVVALLS
                     GLKVLAGTLTVGNMQAFVQYVWQISQPVQTLTQLAPQLQSAKSSLERIFSVLDELDEE
                     DANALELTESLTGQVSFEQVEFGYSEDKPLIRNFNLDVQPGEMVAIVGPTGAGKTTLI
                     NLLMRFYDVTSGSIKVDGQDIRNISRQSYRSQFGMVLQDAWLYEGTIKENLRFGRLDA
                     TDEEIVEAAKAANVDHFIRTLPGGYNMEMNQESSNISQGQKQLLTIARALLADPAILI
                     LDEATSSVDTRLELLIQKAMKRLMKGRTSFVIAHRLSTIQEADKILVLKDGQIIEQGN
                     HESLLADKGFYYNLYQSQFSQSNTLRKSKADVVDLI"
     gene            complement(12421..14157)
                     /locus_tag="VCA20_000552"
     CDS             complement(12421..14157)
                     /locus_tag="VCA20_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1834407.1"
                     /translation="MRLVWTYLKKYPKWIALDLLGAFLFVVVNLGLPTFLARMIDQGI
                     TKNDVSQLYFWAGMMGLIVLLGIVGRVTLAYVAGKLTTNIVKDIRNDLYEKIQDYSHH
                     EYEQIGVSSLVARMTNDAFVLMQFSEMVLKLGIITPLMMIASVIMTLVTSPSLAWTVA
                     VAMPFLVFVIFYVATKTRPLSEKQQKRLDTINQYVRENLMGLRVIRAFTREEFQETRF
                     AEVNEEYTDISKKLFNLTGLTEPLFVQIIIAMIVAIVWFALAPLKTGSLQIGDLVAFI
                     EYSFHALFSFLLFANLFNMYPRMSVSSQRIQEVLDMPISISKNEEGITESDTRGYLEF
                     ENVTFAYPGETESPVLHNISFKAKPGETIAFIGSTGSGKSSLVNLIPRFYDVTLGRIL
                     VDGVDVRRYNLKALRSKIGFIPQKALLFTGTIAENLKYGKMDASLAELHDATDVAQAK
                     EFIESKKEKFDTHLAEGGSNLSGGQKQRLSIARAIVKQPDIYIFDDSFSALDYKTDAI
                     LRSRLKEVTENATVLIVAQRVGTIMDADQIIVLNEGEIVGRGTHNELMESNEIYREIA
                     NSQLNRQSLTEE"
     gene            complement(14659..14901)
                     /locus_tag="VCA20_000553"
     CDS             complement(14659..14901)
                     /locus_tag="VCA20_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEP1834408.1"
                     /translation="MDMIENLIIAIVKPLISQPDSLTIKIVDTPEFLEYHLDLDKSDI
                     GRIIGRKGRTISAIRTIVYSVPTSDKKVRLVIDEKE"
     gene            complement(14923..15195)
                     /gene="rpsP"
                     /locus_tag="VCA20_000554"
     CDS             complement(14923..15195)
                     /gene="rpsP"
                     /locus_tag="VCA20_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEP1834409.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRAPRDGRFIETVGTYNPLL
                     AENSVTLKEERVLEWLAKGAQPSDTVRSLLSNAGVLKKFHEQKFSK"
     gene            complement(15320..15628)
                     /locus_tag="VCA20_000555"
     CDS             complement(15320..15628)
                     /locus_tag="VCA20_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HEP1834410.1"
                     /translation="MKNIMEARKRVEEMEKIFNRLLELNQSLSDSMAQLNQEQSTYLQ
                     LLDYYQSQTYMEDLDLSNKGYFNGIPCGVLSEDGVYNLLFDRTNLASQLRELADMLEQ
                     "
     gene            complement(15628..16275)
                     /gene="pcp"
                     /locus_tag="VCA20_000556"
     CDS             complement(15628..16275)
                     /gene="pcp"
                     /locus_tag="VCA20_000556"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEP1834411.1"
                     /translation="MKIIVTGFDPFGGEPINPALETIKSLPKTIAGVEIILVEIPTVF
                     DKAADVLEEKMAEHLPDAVLCIGQAGGRVDLTPERIAINQDDARIPDNEGQQPIDRTI
                     REDGQPAYFSTLPIKAMVEAIHRIGLPASVSNTAGTFVCNHLMYQALYLAEKQFPKTK
                     AGFLHIPFLPEQVVDKPGLASMSLNDIVRGVEVAIGAIVEYRDKEDIKKGGGSTH"
     gene            complement(16272..17027)
                     /locus_tag="VCA20_000557"
     CDS             complement(16272..17027)
                     /locus_tag="VCA20_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEP1834412.1"
                     /translation="MIVTTSLGMDEGLVYRARRIASELGIEYKERKKQSVGKMLGTYE
                     AVLVLYKDKLILEQRGGQVLFFHPDTAMLRIKSGRDPLLELLGKEKQSIIDCTMGLGS
                     DSIVLASAGHQVTALESSKLVHFIVSRGLQDFDSGLQEVNRAMKSIQTIWTDSLTYLK
                     GQIDKSVDVVYFDPMFSEEIKESQNLSGLSTLADRSRLTEEIVSEAKRVARKKLIIKA
                     HFRDQVFEEFGFKRHVRPNQKFHYGEIILEEEV"
     gene            complement(17091..20270)
                     /gene="carB"
                     /locus_tag="VCA20_000558"
     CDS             complement(17091..20270)
                     /gene="carB"
                     /locus_tag="VCA20_000558"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEP1834413.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYSV
                     VLVNSNPATIMTDKEIADKVYIEPITLEFVTRILRKERPDALLPTLGGQTGLNMAMEL
                     SKTGILDELGVELLGTKLSAIDQAEDRDLFKQLMEDLNQPIPESTIVTTVEEALEFAA
                     EIGYPVIVRPAFTLAGTGGGMCANEEELREIAENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDAADNALVVCNMENFDPVGIHTGDSIVFAPTQTLSDIENQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEM
                     VNPVTGTTYAMFEPALDYVVAKIPRFPFDKFEKGERRLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVYHNEMPELSQVTDDQLVEKIVKAQDDRLFYLSEALRRGYTVEELAQ
                     LTKIDLFFLDKLLHILEIEQELATNFDNIDLLKKAKKYGFADRKIAELWGRTESYIRQ
                     LRTDHKIVPVYKMVDTCAAEFESTTPYFYSTYEWENESIRSEKESVLVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQAAGYESIIMNSNPETVSTDFSVSDKLYFEPLTLEDVLNVIDL
                     EKPKGVIVQFGGQTAINLAEPLAKAGIPILGTQVADLDRAEDRDLFEKALKDLGIPQP
                     PGQTATNEEEALEAARKIGFPVLVRPSYVLGGRAMEIVENEEDLRSYMRTAVKASPEH
                     PVLVDSYIVGRECEVDAISDGKDVLIPGIMEHIERAGVHSGDSMAVYPPQTLSKEVQA
                     TIADYTKRLAIGLNCIGMMNIQFVIKDETVYVIEVNPRASRTVPFLSKVTDIPMAQVA
                     TKLILGQSLAELGYQDGLYPESKQVHVKAPVFSFTKLAKVDSLLGPEMKSTGEIMGSD
                     LTLEKALYKAFEASYQHLAEFGNVVFTIADEDKEEVLGLAQRFYELGYGLLATAGTAA
                     FLTENGLAVTTVGKLCDDSCPDIPSLVRAGKIQAIINTVGKKRVADGDGQVIRSSAIE
                     GGIPLFTALDTAEAMVKVLESRSFMTQAI"
     gene            complement(20539..21618)
                     /gene="carA"
                     /locus_tag="VCA20_000559"
     CDS             complement(20539..21618)
                     /gene="carA"
                     /locus_tag="VCA20_000559"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEP1834414.1"
                     /translation="MSKRRLILEDGTIFEGEAFGADIDVTGELVFSTGMTGYQESITD
                     QSYNGQILTFTYPLVGNYGINRDDFESIKPTCKGVVVSEWARRASNWRNQMTLDEFLK
                     AKKIPAISGIDTRALTKIIRQNGTMKATLANVGDSVEHLTDQLRATVLPTNNIQQVST
                     KTAYPAPGVGRSVVLVDFGLKHSILRELAKRDCNVTVVPYDTTAEEILALHPDGVMLS
                     NGPGNPDDVPEALEMIRGILGKIPIFGICMGHQLFAKANGAMTYKMKFGHRGFNHAVR
                     EIATGRVDFTSQNHGYAVAREDLPECLMITHEEINDKSVEGVRHKYYPGFSVQFHPDA
                     APGPHDASYLFDEFMELMDSFKKGQ"
     gene            complement(22403..23326)
                     /locus_tag="VCA20_000560"
     CDS             complement(22403..23326)
                     /locus_tag="VCA20_000560"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEP1834415.1"
                     /translation="MTITNGKVSLKHLVTMETLSNEEVLGLIQRGIAFKRGEKVELDR
                     KYYASNLFFEDSTRTHKSFEMAELRLDMGMIDFDARTSSVNKGETLYDTILTMSALGV
                     DICVIRHSEVDYYKQLIDSPTIQTSIVNGGDGSGQHPSQSLLDLMTIYEEFGTFEGLK
                     IAIAGDITHSRVAKSNMQILKRLGAEIFFAGPEEWYAEEFDVYGQHLNIDDIVETVDV
                     LMLLRVQHERHDGDGGFSKETYNRLHGLTEERYKRLQDTAIVMHPAPVNRDVEIDDQL
                     VEAPKSRIVRQMQNGVFVRMAILEAIVNGKA"
     gene            complement(23382..23903)
                     /gene="pyrR"
                     /locus_tag="VCA20_000561"
     CDS             complement(23382..23903)
                     /gene="pyrR"
                     /locus_tag="VCA20_000561"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEP1834416.1"
                     /translation="MKTKEIVDDMTMKRAITRITYEIIERNKNLDNIVLAGIKTRGVF
                     IAKRIQERLKQLEGIDVPLGELDTKPFRDDMKVEDDTTNMTANVNDRDVILVDDVLYT
                     GRTIRAAIDNIVSLGRPARVSLAVLVDRGHRELPIRADYVGKNIPTSRSEEIIVHMAE
                     IDGQDAVLLVEGA"
     gene            complement(24102..24749)
                     /locus_tag="VCA20_000562"
     CDS             complement(24102..24749)
                     /locus_tag="VCA20_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HEP1834417.1"
                     /translation="MKHQSILFYSRCLLAILAITGTALEIIKYGIGMLMYYTVQSNLL
                     VSLFAVYMVYAMSKNVDLQTSRFLRIKAAVTMSIMITCVVYHFMLAPLADDFWRLENL
                     LCHYIVPLYFLLDTLIVDRQQQYKWFDPIWWTLLPVLYMIFGLVNGLFIKIPIPDAKD
                     SPFAYFFLNVPKYGWTYVLTYAGVIFVAYLVCGFLLAGLKSINLKTSLSFGKNSQ"
     gene            complement(24797..25135)
                     /locus_tag="VCA20_000563"
     CDS             complement(24797..25135)
                     /locus_tag="VCA20_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002920966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEP1834418.1"
                     /translation="MEILPNCPKCNSEYVYEDGALLVCPECAYEWNPADVAEEESGPI
                     AIDANGNRLVDGDTVTLIKDLKVKGAPKDLKQGTRVKGIRIVEGDHNIDCKIDGFGAM
                     KLKSEFVKKI"
BASE COUNT         7669 a         5720 c         4725 g         7096 t
ORIGIN      
        1 tatctataac catagataca gataatacac actaaaaagg gagtgagcaa gacatccatg
       61 atggctcctc tgtccttacc cacagaataa tgagaatgag cataaagccc tcactaagat
      121 tatataccta gtgagggcct tgtagttggg gcattattga tgttaattat gatgacaaaa
      181 ctgctttacc ctactgataa gaaccacaat atgcgctcaa gaatatgact aaaattcagt
      241 agataggctc tctaatttta agaaggaagg aattgtcata gataatcatg tgtagatcaa
      301 gtcgatattg tcgtttgtat tcccctcaac ttgtccaaag cgttacttag ctacatttga
      361 tacaacgact aaaggtctac gtgcttacca tgattttctc tgtcaatttc atgtggaagc
      421 tgttagtatg gaaagcacag gtgtctattg gcgacttgtc tggcatgttc tatgtgatga
      481 cttcgagcta atacttgctc aaccagccca catgaaggcc attacagaca agaaagacgc
      541 ttactggatt gccaaataaa tgcggattgg tctgcttcct cggagtttcg ttcccgatga
      601 aaccattcaa gaattgaggg agttgaccag acaacgaaaa cagtataata ttgggacttt
      661 tctcatctct ttgttaagac aatctaaaaa atgctaatct ccctcaaata tcctacgaac
      721 ggtatgaatt tgagagattg gaactgcaac gagcaaggaa tcccccatag aaagttggct
      781 atttcctttc acaatccttg acttaccgtg atgaatttga ctggtgatca agcatgaatc
      841 cggcaaacgt aactcgctaa caaatcttct gtccaatgtt tcgttgacaa caacctctat
      901 taaggtcatg tgttcttgct gttctacttc ttctggcaga attttttcca acattgcttc
      961 atagactggc gcgccaccta agaggtccat catcacatag gaactcaaag ctaccaaccc
     1021 gatgaccatc aattggttca aacttccaac catttcagtt actaaaataa tagcagttag
     1081 aggtgctttg gaaatcgctg cgaaaaagcc tgccattccc aatacaataa acaatagcat
     1141 ctgctcctga ctgatgaaac caatatcaag gaagaaccgt gcgacaaaaa gcccaaggag
     1201 acttcctaag gttaatatgg gcaaaaagat gccccctggt agaccagaac cataggaaat
     1261 catgctccat acaaatcgta ttaaaatcaa gattgccaat gtcagtagtc cttgttccaa
     1321 atatggaagc tctaaaatca aatgatgccc gccccccaac agattaggga aaaaataacc
     1381 aatcggtata ataaacaaaa aggcaaatac actgtagtat ggaactggga gaggaatgat
     1441 ttttgagagg agggtataga aaccttgtat attgaggata acaacttcat acaaatatcc
     1501 agcaagtcca aggaaaactc caagtaagat atagatccaa tattggtcaa gagatagtaa
     1561 ctgtaagtcc ttcggcatat gcaaaacagg cattaatcca aaaacatgta aggaaataaa
     1621 gttcgcagtg atactggctg ccagggctga gacccatacc agacgtgaaa actgatgata
     1681 gacttcctcc acaacaaata aaaggcctgc tattggtgca ttgaaagctg ctgccaaacc
     1741 tgccgcagcg ccactagcaa tcaaactcct tctttccatt tcactggatt taagaagaac
     1801 tgatacacct ttagccgtta ccgctcctaa ttgaatactg ggaccttcac gccccagcat
     1861 tagaccagat gcaatggata aaacaccgcc gataaatttt ctccaaagga ctgaccacca
     1921 gtttaaatcc ataattcctt ttagttctac ctccacctga ggtataccag aaccttttgc
     1981 atttggctcg tctttgatca acttgccaac aagtaaagct atgaccagat aaagccctcc
     2041 gataagaagt aaatagattg cattgtctgt tgccagagaa tagaggtgcg taaagacaga
     2101 aaatagtttt tcaatggcta agcgaaatgc aacgaccact aaaccagcga caatgccaac
     2161 taaactacca cgcagaaccg aatagaggat tgattgagaa ataaaactaa cttctttccg
     2221 gtgattttcc attgggattt cccttccttt ctatagtatg tatgaccaaa aaataggagc
     2281 aataagtact tagtaaagaa cctcactccc attcttttat ttatattgct acctgtccaa
     2341 tcagactatt tccgatacat cttgaattgg agatagaggt cattatacat tccaagtatc
     2401 tctggtccaa gattatcata aacttccatc ttcttcatcg tttcttcaga tggatagaaa
     2461 gattcatcgt tctgagtctc ctcatccagc atagccttgg ctgcaggaat tggtgttgaa
     2521 taaccaacat attctgcatt gcgaagcgca ttttcaggac gaagcataaa actcataaag
     2581 gcgtaagctc catcaatatt ttttgctgtt tttggaatga ccatgttatc gaaccaaaga
     2641 ttagaacctt ttgatggtac aacatagcgc agattttcat ttccatctaa catttcactg
     2701 gcttcaccag agaaggatac cgcaatcgct gctgcatctt gtatcatata accttttatc
     2761 tcatcagcaa caattgcttt gacatttgga gttaaattat aaagatgttc ggctgccttt
     2821 tcaatctcgg taggattttt agagtttaag ctgtaaccca aagattgcag accgaagccc
     2881 atgacctcac gaacgccgtc gattaacatg atattgtcac ggtactcctc tgaccacaag
     2941 tcttcccatt cctgaggagg attttctacc atggtttcat tgtaaacaat gcccaaggtt
     3001 ccccagaaat atggaataga ataatcatta ttcttgtcaa agctaagtcc cataaatcgt
     3061 ggatcaatat tttctatccc ttcaatctta gacttatcta gcttgattaa catattttct
     3121 tctgtcatct tagaaatcat gtactcagat gggattgtca aatcgtatgt tgttccaccc
     3181 tgcttaatct tggtgtacat cgattcattg gagtcaaagg tctgataatc tacctgaata
     3241 cctgtttcag cagtgaattc tgcgagtaat tctgggtcaa tataatcacc ccagttatag
     3301 atgacaagtt tatcagttgt cccttgggtt gaatcagctt ctaacttata actaattccc
     3361 cataaaacta ggacaatagc tataattcct acaaaaaatg aatacaatcg tttcattagt
     3421 tggcctcctt ttcacgtgag atgaaataat aacccactac caataagata gagaaaagga
     3481 aaacaagggt tgatagggca ttgatttcca aagaaatccc ttgacgagct cgtgagtaaa
     3541 tctctacaga taggtttgag taaccatttc ccgtaacaaa gaatgtcacg gcaaagtcat
     3601 ccaaggaata ggtaaaggcc atgaagtagc ctgcaatgat ggctggtgtt aggtatggca
     3661 acatgatttc tttcagcatt tgtggctgag ttgcccccaa atcataagct gctgaaatca
     3721 tatcggcatt catttccttc aagcgtggca aaaccatcaa tactacgatt ggaatagaaa
     3781 aagcaatatg actgagcaat actgagacaa agccaagctg gaaaccaatc atggtaaaga
     3841 gaattaggaa actcgcccca atcatgacgt ctggagctac catcaagata ttattgacag
     3901 acaagagtgt attttgaaaa cgaggctttg cctgatagat atagatggcg ccgaaagttc
     3961 cgataagggt cgcaatcaaa gaactcaaaa aagctaatag gaaggtttga gctaagatga
     4021 gcatgagacg gctatcgcca agcatagatg aaaaatgctc caaggtaaag cctgtaaagc
     4081 cattcatatc tccgccttca ttaaaggcat aaaaaatgag atagaaaatc ggtaagtaga
     4141 gaaggagaaa ggcaactgtt aagtagatat ttgcaaattt cttcattatt tcttcctctc
     4201 tttcgtcatc cacataatca agagcatggc taaaatcaag accacaccta tggttgaacc
     4261 cattccccag ttttgtgttg tgagaaagtg ttgttcaatg gctgttccca gtgtaatgac
     4321 tcgattacca ccaattaaac gggtcaacat gaagaggctc agacttggaa tgaaaaccgc
     4381 ctgtacacca gaacggacac cattcataga aagaggaaaa ataacttttg tgaaggtctg
     4441 ccaaggagtt gcccccaaat cacgactggc attgattagg tttttatcca aatcatccaa
     4501 ggcgttaaag atcggcaaaa taataaacgg aatctcaata taggctgcaa cagcaataaa
     4561 ggagaaatct gtgaaaagaa tttgctgagc tcctagacca ataaattcta ggaactggtt
     4621 gatagagccg tgttgcccaa aaataccgat aaaggcatag gctttcagca aaagattgac
     4681 ccaggttggc aagataatca acatgagcca gagttgtcta tgcttgagtt tggtcaagaa
     4741 ataggctgtt gggtaggaaa ccaaaagcgt gaccagagta ataatacctg cgtaaaaaat
     4801 agagttaaaa ctcattcgca gataggtcat atttggcgag ttaaaatagg tctggtaatt
     4861 ggccaaagtg aaattgccat gaatatcaaa gaaagatttg aagataatca aggcaactgg
     4921 agcgagtaca aagagaaaga tccatagggc atagggaatt gcaaaaagcc tagaggtttt
     4981 cttcattgcg ttcctcctca atggcgttaa tcaagccatc ttctacttct tctacttcca
     5041 catattcttc gatacgggca tcgaattctt cctctgtttc attgagacgc atgatatgaa
     5101 tatcttccgg ctcaaaactt agtccaatca cttctccgac aatggcctta cgagttgagt
     5161 gaatcatcca ctcattaccg atgtcgtcat aggcgatgat ttcatagtga acccctcgga
     5221 agagctgagt atccactttt acctgcaatt tgccctcttc aggtaaggta atctgcaaat
     5281 cttctggacg gataacaact tcaacttctt cattaggacg cataccacca tcgacagctt
     5341 caaagcgttt gccgttaaac tcaaccaagt agtcttctat catgcgacct ggaaggatat
     5401 tggattcacc gatgaaggta gcaacaaaat ggttgattgg ctcgtcgtaa atgtccactg
     5461 gcgtcccaga ctgaacaatt tcaccttcat tcatgacaaa aatccagtca ctcatggcca
     5521 gagcttcttc ttggtcatgg gtaacgaaga cgaaggtaat tcctagacgt tgttgcaatt
     5581 cacgcaattc gtactgcatt tctgttctta gtttcaagtc cagggcagaa agtggttcat
     5641 ctagcaagac aacgcgaggc tggttgataa tcgcacgtgc aatagcaacc cgctggcgtt
     5701 gaccaccaga caatttctga atggaacgat tttcataacc agaaagtcgg accatttgta
     5761 aggcttctgt cacacggcgt tcgatttcag ccttgtcaac ctttcgtaat ttaagaggaa
     5821 aagccacatt ttcaaagaca gtcatatgtg ggaaaagagc atatgactgg aaaacagtat
     5881 ggacatcccg tttattggtc ggaacatcat taatccgttg accgtccaga taaatatcac
     5941 ctgaactcgc atccaataaa ccagcaataa tgttcaaaat cgttgatttc cctgaaccag
     6001 aagctcctaa aagggtataa aatttcccct cttccaattc aaaactaata tcctttaata
     6061 cagttgttcc actgtcttca aatacttttg aaacgttctt aaattcaata attggctttt
     6121 tcaattctca taaattcctt cttttttgta agagcctgat agctagagtg tatcagcagg
     6181 atcgccgata atacgaacct ctctctccag agtaattcct gaagattttt caacaacttc
     6241 aattacatgg gcaatcaaat tttcgtaatc agatgctgta ccattttcta tattgaccat
     6301 aaagccagcg tgtttttcag aaacttcgac tccaccaatt cgataacctt ttaaacccgc
     6361 atccataatc aactgtcctg cgaagtgacc aagtggacgc ttgaagactg aaccacaaga
     6421 tggatattcc aaaggttgct tgagctcgcg taaatgtgtc aaacgagcca tttcttgttg
     6481 aataaccgta tgatttcctg gcttgagggc aaattttgca gacaaaacaa tcgcaccgtt
     6541 ttcctgcaaa atagaagaac ggtagccaaa tcgtaactca cgattgtcca atgtttcgac
     6601 ataacctgct ggagtcaaaa tctgcgctga aactagaatg tgtgccactt cacctccgta
     6661 agcacctgca ttcatataga cagcaccacc aatgcttccc ggaataccac aagcaaattc
     6721 aaatcctgat aaagaatgga aaagtgcaac ttttgttgtt tcaatcagat tggcacctgc
     6781 ttccacttca atggtgtatc ctgatacagt aacggagtta agtttatcca tacggataac
     6841 aaaaccacga ataccgccat cgcggacaat gatattactt gaatttccta atacttgcca
     6901 tggaatacct tcgcgtttag caaattctac tatacgaaca atctcgtaac gattccgcgg
     6961 aaaagctaga taatcaacag ctcctcctac ttttgtatag gtatattctt ttaaaggctc
     7021 atcgaatcga atatcgatgc cttctaattc aagcctaatt ttatctttca taaccttctc
     7081 atttcaattt aagatataca ggatttatta taccaaaaat tacaggagaa aacgaataaa
     7141 attcaacaaa aatactttca caagccaaat taatttgccc caatattttg aattggagaa
     7201 tagaatgcac aaaaaaggag ttcccttcct accgcatata gtagcgatgg cgaactccaa
     7261 taaaataaac atgtttagtt ttgcaaatga cagattctca ctatctatac aatagcaatc
     7321 cctttggtat ctacttgaag gcggtggact gttccgtcta gatttagttc ctctaatgct
     7381 agaacaatag cgtcctccct atctttgggc gctaatacca tcactgttgg accggcacct
     7441 gacaagtagg ttgcataggc tcccaactcc tgtgcagttt gcttgattgg acaaaactcc
     7501 ttaacaagta gctgacgaaa cggttcatgg aacatgtctg actgaattgc tttcccagct
     7561 ttttccagat cacctgccat caaactggca acagcaacgt ttgcaatggc actagcagcc
     7621 actgctttct tgtaccccat ctgagaaggc agtacacctc ggctttcaac cgtacgcagt
     7681 gggtagtttg gaataaaggc aacaaaactt gcttctggga attctgttac aactgcctgt
     7741 actctcttat taacatagct agagatgact aaattaccaa aaatcgctgg ggctacatta
     7801 tcaggatgtc cttcaatttc tgtagctttg agcaattttt catccgctgt tatattcaaa
     7861 cctgctaatt gattagccaa ttcgatacca gctacgataa cggaactgga tgaccccaaa
     7921 ccacgtgcca agggaatatc actaatcatt ctgatacgac gcggctgcaa tcctttttca
     7981 atctttaaag ctgtcttgat caaaagattg ttcttgtctg atggaacatg ttcaagattg
     8041 tgctcaatca tccattcatc agtctcttca aacacttcaa tcgttagata tttcgaaagg
     8101 gcaacaccta ctgaatcaaa tcctggaccg atattagctg aagttgctgg aataataatt
     8161 ttcattgctt attctcccag tactttaaag gtatttaaga cagtaaagtc tgtttcttta
     8221 ctcaatttat ctgttacatt ttccaactga gtcttactca tttcatgggt cacaatcact
     8281 aggcgagctg tttctccatc tgcagcttgt tgcagaattt gcttgaagga aatttcttca
     8341 gaattgaaga tttctgctaa acgaagcatt tttccatttt tatcaggcgc atcaattgag
     8401 aaataatatt cgctcttcac atcgcttggg gccgccaatt gaagtgggcg actgtattca
     8461 ttgaaggctt taccgatagt tttatctttc aagcgacgaa cgatacgaat aatgtcagcc
     8521 ataacacttg ttgcagtcgg tttttgccca gcacccggtc cattgtacat agactggtcg
     8581 atgccgatag attcgacaaa tacagcattc atcacaccat tcacgcttgc aagtggatga
     8641 tttttcggta ggaaggttgg agatacttcg gcagcaatac cagaggtagt ttcttgaata
     8701 tcacccacaa gtttgatgac atagcccaat tcttgtgcca ctgctacatc ttctggcgtg
     8761 atatttgaaa tacccttgtg tgctacgtgt tcaaaatcaa ctgtcatccc gaaagcaaat
     8821 tgactgagga ttacagcctt gtaagccgca tcaatcccct caacatcatt tgttgggtca
     8881 gattctgcat aaccaagttc ctgtgctgtt tgaagagctt tttcgtaagt ccagccttca
     8941 tcaaccatct tggtcaacat gaagtttgaa gtaccattca atacaccgag gatacgagtc
     9001 accttgtcag cagccaatga gttaaccaag gtacgcaaga ttggaatacc acctgcaact
     9061 gcagcttcat aataaagtgc cagccccttt tcttcagcca aggcacgtaa ttcgctacca
     9121 tgaacagcaa gtaaatcctt attggctgtt acaatatgct tgcctgtttc caaggcacgt
     9181 gtaataaatg tttttgctgg ctcaatacgt cccatcagct caacgacaat ggcgatgtct
     9241 tcgtcagcca aaatgtcatc aatatttgtc acaaaatgat agtcatgacc tgtagccaag
     9301 agacggtctt tttctgcttc atccttgacc aaaaccttag caacttcaat actatcatgc
     9361 gcggctttct ctattttttc ctggttttct tctaataaaa atggcacacc actcgcaacg
     9421 gttccaaatc ctaataatcc tatcttaact accatgtgct ctcctttaaa atgtatttct
     9481 cccattataa caaatattct gactatttgg caaaaaattt gatataattg ttttaatata
     9541 tgattatgga gataagataa tgaaacattc aagaagagcc cgtcgaagcc gcacacaaaa
     9601 gaaacaactt gtgccaatgc tacttggtat cgcattatta gtagtgactc tactatctat
     9661 ttttctaatc tttctaaaac agccatctaa gcagcaaagt gattccaaag cttcaacggt
     9721 agagacaaca accagttcga gtgctatgac ttcagaaagt agtgaagtac aaacagaaat
     9781 catttgggaa cagcaaactg aacctatcaa acttccaatc ctcatgtatc atgccattca
     9841 cgtaatggct cctgaggaag aagctaatgc gaatcttatc gtagatccaa cgactttcga
     9901 aagtcatcta aaagcattgc aagatgcagg ttactatact ttatcacctg aagaggctta
     9961 taaggtattg acagaaaatg tccttcctgc tggcaaaaag gttgtctggc taacctttga
    10021 cgatagcctc tgggattttt atagctatgc ctacccattg cttaagcaat atcaaatgaa
    10081 ggcaaccaac aatgtcatta cagggtttac tgaatatgga caagcagggc accttactct
    10141 tgatcaaatc aaggaaatgc ggaccgcagg gctctccttc caagggcata ccgtcaacca
    10201 ccctgattta gagtatagtt ctatcgagga tcaaacaaat gaacttactt cctctaaagc
    10261 ctatcttgac agccaattaa atcaggaaac catcgctatc gcctaccctg gtggacgata
    10321 ctcagcagat accgtagcct taactgaaca agctggctac aaacttggtg tgactactaa
    10381 caacggctta gcttctgcta gtgacggatt actgactctc aatcgtgtcc gcatcctccc
    10441 aaccactacc gccgagggac tattatccga aatttcctat taattttcac aaacatgtat
    10501 atgagaaaac tccctatcac tgacaaggga gttcgtttta tgtatactca atgaaaatca
    10561 aaatcaggct aggcgacgaa gatgcagata gaactgaagt tcatcaaatc aagtcaacaa
    10621 cgtctgcttt tgattttcga agagtattag attgactaaa ctgactttga taaagattat
    10681 aatagaaacc tttgtctgcc aaaagacttt catggttacc ttgttcgata atctgtccat
    10741 ctttcaagac caaaatctta tcagcttcct gaatggtaga taagcgatga gcgataacga
    10801 aacttgtccg ccctttcatc aaccgtttca tggctttttg aatcaatagt tccaaacggg
    10861 tatcaactga agaagtcgct tcgtcaagaa tcagaatagc aggatccgcc aacaaggcac
    10921 gcgcaatggt caaaagctgt ttctgacctt gggaaatatt gctggattcc tggttcattt
    10981 ccatattata accgccaggc agggtacgga taaagtggtc aacgttggca gctttagcag
    11041 cctccacaat ttcctcatcc gtcgcatcca ggcgtccaaa acgcaggttt tccttgatgg
    11101 tcccttcata gagccaagca tcctgcaata ccataccaaa ttgactacgg taggattgac
    11161 gggaaatatt tcgaatgtcc tgaccatcca ctttgataga accggatgtg acatcataga
    11221 aacgcatgag caagttgata agagtagtct tacctgcccc tgttggacca acgatggcca
    11281 ccatctcacc gggttgaaca tccagattaa agttacggat aagcggcttg tcttctgaat
    11341 agccaaattc gacctgctca aagctgactt gtcctgtcag actttctgtc aactctagtg
    11401 cattggcgtc ttcttcatca agctcatcta ggactgaaaa gatgcgttcc agggaggatt
    11461 ttgcagactg taactgtggt gccaattggg tcaaggtttg gacgggctga gaaatctgcc
    11521 agacgtattg gacgaaggcc tgcatattac cgaccgtcaa tgtgcctgca agaactttca
    11581 atccagatag cagggcgacg acaacatagg cgatgtttga gaagccgtga actagaggca
    11641 tgagtaaacc agacataaaa ctagccttga aaccaacttc ctgcaaatct gcggtaatct
    11701 ggcgaaactc ttctgtagaa atttcttcac ggccgtaaag tttgagaacg ttaaatccag
    11761 tcagattttc ttggacaaag ccgttcaaac gacctagggc atcagcctgc tgcttgaaat
    11821 atggctggga tttgcccatg acaaacttag aaccaaaata ggtcactgga accaaggtta
    11881 cgactacaag tgctagggat acatccaacc agaaacacat gaaaatggct agagaaaggg
    11941 tcagaacggc attgactagc tggagaaaac tttgttgcag ggcgttcgac acagtctcca
    12001 catcgcttgt aaaacgtccg agtaaatcac catactggtg cttgtcaaaa taagatactg
    12061 gaatcttgtt aattttgtgg ctcaaatcct gacgtagttc cttagttgct ccctgcactg
    12121 ccttggtcat aaaatagtta gaaccgtagg caccgatttc atatagaagg gcgcgaaggg
    12181 catagatggc aagcatgatg gcaatatagg aatagttaat ctgcgctcct tctactccat
    12241 tagcaatatc caacagattc ttggtcaatt ctgtaatgat aagccccagt acaaagggct
    12301 ccaaggcatt cataactgcg ctaatggtct ttagaaaaac agctaaaaat acagaagatt
    12361 tgtaacgacg taaatagtcc caaacacggg taaatactga tctattcttc ataattcctc
    12421 ctattcttct gttaacgatt gacggttaag ctgagaattg gcgatttctc gataaatctc
    12481 attgctttcc atcaattcat tgtgggtgcc gcggccaacg atttcacctt cgttcagcac
    12541 gatgatctgg tcggcatcca taatggttcc gacacgttgt gccacaatta gcaccgtcgc
    12601 attttcagtc acttccttca aacgactcct caaaatcgca tccgtcttat aatccaaggc
    12661 tgagaaagaa tcatcaaaga tataaatatc gggctgtttg acaatagcac gtgcaatgga
    12721 caaacgttgc ttttgcccgc ctgacaggtt gcttccacct tctgctagat gggtgtcgaa
    12781 tttctctttc ttactctcga taaactcctt agcctgagcc acatccgtcg catcatgaag
    12841 ttctgcaaga ctagcatcca tcttaccgta ctttaagttt tccgcgatag ttccggtaaa
    12901 tagcagagct ttctgtggaa taaagccaat cttgctgcgg agtgccttta gattgtagcg
    12961 tctgacatcc acaccgtcca ccaagatacg accaagtgtc acatcataga aacgtggaat
    13021 caagttgacc aaggaagact taccagaacc tgttgaacca ataaaggcaa tggtttcacc
    13081 aggcttagcc ttaaatgaaa tattgtggag aacaggcgat tccgtttcac caggataggc
    13141 aaaagttaca ttttcaaatt ccaaataacc acgagtatct gattcagtaa taccttcttc
    13201 attcttagaa atggaaatgg gcatgtccaa gacttcctga attcgttggc tcgataccga
    13261 catacgtgga tacatgttaa acaagttagc aaagagcaag aaagagaaaa gcgcgtggaa
    13321 actatattcg ataaaggcta ctaaatcacc gatttgcaga ctgcctgttt tcaagggagc
    13381 caaagcaaac cagacaatgg ccacaatcat agcaatgata atctggacaa agagaggctc
    13441 tgtcaatcca gtcagattaa agagcttttt ggaaatatcg gtatattcct cattgacctc
    13501 tgcaaaacgg gtttcttgaa actcctcacg agtaaaggca cgaatgacac gtaagcccat
    13561 gagattttca cgaacatatt ggttgatcgt gtccaaacgt ttttgttgct tttcagaaag
    13621 aggacgagtt ttggttgcca catagaaaat aacaaaaact agaaaaggca tggctactgc
    13681 tactgtccag gccaagctcg gactggttac caaggtcatg atgacagaag caatcatcat
    13741 caagggtgta ataataccta gtttcaacac catttcagag aattgcatga gaacaaaagc
    13801 atcatttgtc atacgagcaa ctagagaaga aacaccaatc tgctcatatt catgatgaga
    13861 atagtcctga attttctcat acagatcatt tcgaatatct ttgacgatat ttgtagtcaa
    13921 ttttccagct acataggcaa gtgtcactcg accaacaatc ccgagcagga caatcaatcc
    13981 catcatcccc gcccagaaat agagctgact aacatcgttc tttgtaattc cttggtcaat
    14041 catcctagcc aaaaaagtcg gcaagcctaa gtttaccact acaaacaaga aggcccccaa
    14101 caagtctaag gcaatccact taggatattt tttcaaatag gtccaaacta atcgcataaa
    14161 tccacctttc tatttgtcac ttcactgagc gacataaaat aagttaagtc tgacatttgc
    14221 aaacccaatt caataaaatt gatacttaat gaaaatcaaa aacagactag gcgacgcaga
    14281 tgcagataga actgaagttc atcaaatcaa gtcaacaacg tctgattttg attttcgaag
    14341 agtatgagat tccaaactag tataagggag gggctatctt ccagagagac tagctttgga
    14401 aaatgagttt ctcgtcatca ttgacctgcc cttacccttt tatatgataa aaagggagtg
    14461 ggaaagaact cgactggcca aaaaagagtt cgtcttccca cccccgcaca gttgattagg
    14521 tcggatttgg agtgtaaaac acgaacaaat ctgccaatca accactgtgc tgagatgttg
    14581 acacgaaccc tgagaagcga ggttagtctt tttccccaac cccttctgta aagttaaaag
    14641 ccaccaaacg gtgacttttt attctttctc gtcgatcact aaacgaactt ttttatcact
    14701 tgttggaaca gaatagacaa tcgttctgat tgcagaaatt gtgcgtcctt ttctaccaat
    14761 gatacgtcca atatcagatt tatccaaatc caaatgatat tccaagaatt caggtgtatc
    14821 aacaattttt attgtcaagc tatcaggctg tgaaatcaaa ggtttcacaa tcgcaataat
    14881 gagattttca atcatgtcca taagtgttct cgcttactgt tcttacttag agaatttttg
    14941 ttcgtggaat ttcttcaata cgccagcgtt tgaaagaagg ctacgaactg tatcagatgg
    15001 ttgtgcacct tttgccaacc actcaagtac acgctcttct ttaagagtta ctgagttttc
    15061 agccaaaagt gggttgtaag taccaacagt ttcgatgaaa cgaccatcac gtggtgcacg
    15121 tgagtctgca acgttgatac ggtagaaagg tttcttttta gaacccatac gagtcaaacg
    15181 gatttttact gccattttta aatgttctct tttctttgta tttttattcg gtgaaatagc
    15241 agagctattt agcacatgtt ctattatacc acactttaac agggtgtcaa gaaaaaaact
    15301 tgacaggttt tgaaaaattt tattgttcga gcatatctgc tagttctctc agttgacttg
    15361 ctagatttgt acggtcaaac agtaaattat agactccatc ttcactcaat actccgcaag
    15421 gaataccatt gaaataaccc ttattagata aatcaagatc ttccatatag gtctggctct
    15481 ggtaataatc taataactgt aaataagtcg attgttcctg gttaagttgt gccatactat
    15541 cggaaaggga ctggttcagc tctagcagtc tgttaaatat tttttccatt tcctctacac
    15601 gttttcttgc ttccataata tttttcatca gtgtgtactg ccaccaccct ttttaatatc
    15661 ctctttatcc ctatattcaa caatcgctcc tatagctact tcaactcctc ttacaatatc
    15721 gttcaaagac atggaagcca gtcctggctt gtcgacaacc tgctctggca agaagggaat
    15781 atggagaaaa ccggccttag tctttggaaa ttgtttctcc gccaagtaaa gagcttgata
    15841 catgagatgg ttgcagacaa aagtcccagc agtattagaa acagaagctg gaagcccaat
    15901 cctatgaatc gcttcaacca ttgctttgat gggtaaggtg gagaaataag caggttgacc
    15961 atcttctcga atcgttctat caatcggctg ttgcccttca ttgtctggaa tgcgtgcatc
    16021 atcttggtta atggctatac gctcaggagt taaatcaact cgcccaccag cctgaccaat
    16081 acatagtacc gcatcaggta aatgttctgc cattttctcc tctaaaacat ccgcagcctt
    16141 gtcaaaaaca gtcggaatct ctacaagaat aatctctaca ccagcaatgg tcttaggtag
    16201 agatttgatt gtttccaagg ctgggttaat aggctcgcca ccaaagggat caaagcctgt
    16261 tacgatgatt ttcatacttc ctcctccaaa ataatttctc cgtagtgaaa tttttgattg
    16321 ggtctgacat gacgtttaaa gccaaattct tcaaagacct gatctcgaaa atgagccttt
    16381 attatcaatt tcttcctagc tactcgttta gcctcagata caatttcctc cgttaaacga
    16441 cttctgtcag ccaaagtaga caagccagaa agattttgtg attccttgat ttcttctgaa
    16501 aacatgggat caaagtaaac gacatcaaca gacttatcta tttgcccttt caaataagtc
    16561 aaactatctg tccaaattgt ctggatagat ttcatggctc gattgacctc ttgcagccca
    16621 ctatcaaaat cctgtaaacc tcgactcaca ataaagtgca ctagcttaga actttccaag
    16681 gctgtcacct gatgaccggc acttgctaac acaatgctat cagatcccaa gcccatagta
    16741 cagtctatta ttgattgttt ttcttttcca agtaattcta aaagcggatc tcttccagac
    16801 ttaatccgca acatggcagt atctgggtgg aaaaataaaa cctgcccacc cctctgttct
    16861 agtatcaatt tatccttata aagaaccaag acagcctcgt aggtacctaa catttttcca
    16921 accgactgct tctttctttc cttgtattca attcccagtt cactggcaat ccgtctagcc
    16981 ctatagacca gcccctcatc catcccaaga ctagttgtta caatcatttt catactcttc
    17041 agtatatcaa aaaatcagga cgaactcaaa gctcatcctg atagtatctt ctatatagcc
    17101 tgtgtcatga agctacggct ctccagcact ttgaccatag cttctgctgt atcgagggcc
    17161 gtaaagagcg gaattcctcc ttcaatagca gagcttcgaa tgacttgacc gtcaccatct
    17221 gccacccgtt ttttcccaac cgtattgata atagcctgaa tcttacctgc ccgaacaaga
    17281 ctaggaatat ctggacagga atcatcgcat aatttaccaa cagttgttac agcaagtcca
    17341 ttttctgtta gaaaggcggc tgttcctgcc gtagccagta ggccataacc aagttcatag
    17401 aaacgttgag ccagaccaag cacttcttcc ttgtcttcat ccgcaatagt aaagacaacg
    17461 ttgccaaact cagccaggtg ctgataactt gcctcgaaag ccttatacag tgctttttct
    17521 agtgtcaaat ctgagcccat aatttcacca gttgacttca tttctggtcc taataaacta
    17581 tccactttgg caagtttagt aaaggagaat actggcgctt ttacatgcac ttgtttgctc
    17641 tcaggataaa gcccgtcttg gtagccaagt tccgcaaggc tttgtcccaa aatcaatttg
    17701 gttgcaacct gagccattgg aatgtctgtg actttggaca agaatggcac cgtacgactg
    17761 gcacgaggat tgacctcaat gacatagacc gtttcatcct tgatgacaaa ctggatattc
    17821 atcataccga tacagttgag accgattgcc aagcgtttgg tgtagtctgc aatggtcgcc
    17881 tgcacttctt tggacaaggt ttgtggcgga taaacggcca tagagtcccc tgagtgaacc
    17941 ccagcccgct cgatatgctc cataataccc ggaatcaaga catccttacc atctgaaata
    18001 gcatccactt cacactcacg accgacgata taagaatcca ccaaaactgg gtgctctggc
    18061 gaagccttga ccgccgttct catataagaa cgtaagtcct cttcattttc gacaatctcc
    18121 atagcacgac cgcccaagac ataagaggga cgaacaagta ccgggaaacc aattttgcgg
    18181 gccgcttcta gagcttcctc ttcgttggtt gcggtttggc ctggtggctg tggaatccct
    18241 aggtctttca gggctttttc aaataaatcc ctgtcttctg ctctatccaa atccgccacc
    18301 tgtgtcccca agattggaat acctgctttg gccaatggct ctgccaaatt gatagccgtc
    18361 tggccaccga actgaacgat aacccctttt ggtttctcaa ggtcaatcac gttcaaaaca
    18421 tcttctaaag tcaatggctc gaagtaaagc ttgtccgaaa ccgaaaagtc agttgacaca
    18481 gtttcagggt ttgagttcat aatgatggat tcgtagccag ctgcctgaat agccttgacc
    18541 gaatgcaccg ttgcatagtc aaactccaca ccctgtccga tacgaattgg tccagaaccc
    18601 agcaccaaaa cagattcttt ctccgaacgg atggattcat tttcccactc ataagttgaa
    18661 tagaaatatg gcgttgtact ttcaaattca gctgcacaag tgtccaccat cttgtaaacg
    18721 ggtacaatct tgtggtctgt tctcagttga cggatgtagg attcagttct gccccagagt
    18781 tccgcaatct tgcggtctgc aaaaccgtat ttcttggctt ttttcaataa atcaatatta
    18841 tcaaaattcg ttgccaattc ctgttcgatt tccaagatat gcaatagctt gtcgaggaag
    18901 aaaagatcaa tcttcgtcaa ttgagccagt tcttctactg tataaccacg tcgaagggct
    18961 tctgaaagat agaataaacg atcatcctgt gccttgacaa tcttttcaac caattggtca
    19021 tctgtcactt ggctgagttc cggcatttca ttgtgataga caccgatttc taaagaacgg
    19081 caggccttaa gcagactttc ttcgatatta cgcccaatgg ccatgacctc gccagttgcc
    19141 ttcatttggg ttccaaggcg acgctctcct ttttcaaact tgtcaaatgg gaaacgtgga
    19201 attttagcca caacatagtc aagagctggc tcaaacatag cataggttgt tccagtaaca
    19261 gggtttacca tttcatccaa ggtcaaacca accgcaattt ttgcggctaa tttggcaata
    19321 ggataacctg tcgctttcga tgccaaggca gaggaacgcg atacacgagg attgacttcg
    19381 atgacatagt atttgaaact atgcggatcc aatgccaact gcacattaca cccaccttcg
    19441 attttgaggg cgcgaataat gctgagactg gcatcacgca acatctggtt ttcaatatcc
    19501 gaaagcgtct gcgttggggc aaatacgata gaatctcctg tatgaattcc cacagggtca
    19561 aagttttcca tgttacatac gaccaaggca ttgtcagctg catcccgcat cacttcgtat
    19621 tcgatttctt tgaaaccagc aatcgaacgc tcaatcaaac actgggtcac tggtgacaat
    19681 tttagaccat tttctgcaat ttctcgtagt tcttcttcat tggcacacat accaccgcca
    19741 gtaccagcca acgtgaaggc tggacgaaca atgacaggat agccaatttc agcagcaaat
    19801 tccaaggctt cttcaactgt cgtcacaatc gttgactcag gaataggctg attgagatct
    19861 tccatgagtt gtttgaagag gtcgcggtcc tctgcctgat caattgcaga cagtttagtt
    19921 cccaacaact caactccaag ctcatctaaa ataccagtct tagacaattc catggccatg
    19981 ttgagaccag tctgaccacc aagagttggc aagagagcat ctggacgctc ctttcgtaaa
    20041 atccgtgtga caaattcaag cgtaatcggc tcaatataaa ccttgtccgc aatttccttg
    20101 tctgtcataa tggtcgcagg gtttgaattg accaaaacaa cactatagcc ttcttccttc
    20161 aaagctaaac aagcctgagt cccagcataa tcaaactccg cagcctgacc gataataatc
    20221 ggaccagacc caatcaccat aattttctta atatccgtac gttttggcat aatgtatgat
    20281 acaaagcccg ttaagaggac aaagcgaaaa taggaaacac gacactgatg cttcaagcat
    20341 caagacgacg tttatctttt tcgcacagtc cttagggcgt gttcgaatag gaaaatcaaa
    20401 tcttattcga acctgtacct tctcctttct attcgtcgcc tctctgagcg acattaaata
    20461 agataagtcc gacgtttagc atgaagtccg acttgctctg accgacgcag taaggtttag
    20521 ctcgatatta gttaaacctt actgcccttt tttaaacgaa tccatcaatt ccataaattc
    20581 gtcaaacaaa tagctggcat catgtggacc aggtgcagca tctgggtgga attgaacaga
    20641 gaaacctgga tagtacttgt ggcgtacccc ttctactgat ttgtcattga tttcttcatg
    20701 agtaatcatt aaacattcag gtaaatcttc acgagccact gcataaccgt ggttctgact
    20761 ggtaaagtcc actcgacctg tcgcaatttc acgaacagca tggttgaaac cacggtgacc
    20821 aaacttcatt ttataagtca ttgccccatt tgctttagca aagagctggt gccccataca
    20881 gataccaaaa attggaattt tcccaagaat cccacgaatc atttctagcg cttctggaac
    20941 atcatctgga tttcctgggc cgtttgatag cataacacca tcaggatgaa gagcaagaat
    21001 ttcttcggca gtagtatcat aagggacaac tgtcacattg caatcacgct tggctaactc
    21061 tctaagaata gaatgtttca atccaaaatc aaccaaaaca acactacgtc caactccagg
    21121 tgcaggatag gctgtttttg tcgatacttg ctggatgtta ttggttggca aaacagtcgc
    21181 tctcaattga tccgtcaaat gctcaacgga atcaccgaca tttgcaagag tcgccttcat
    21241 ggtcccgttc tgacgaataa tttttgtcaa ggcgcgtgtg tcaattccag aaattgctgg
    21301 aattttctta gcctttaaaa attcatccaa agtcatctgg ttacgccagt tacttgctcg
    21361 acgagcccac tcgctaacaa ccactccttt acaagtcggt ttgatcgact cgaaatcatc
    21421 acggttaatc ccgtaatttc ctaccaaggg ataggtaaag gttaaaatct gaccattata
    21481 agactggtcc gtgatagatt cctgataacc tgtcatccca gttgagaaaa ccaactcacc
    21541 agtcacatca atatcagcac cgaaggcctc cccttcaaaa atagtgccat cttccaaaat
    21601 taaacgtctt tttgacataa taataagata ttaagggcgt taagcggaca cagtcaaaat
    21661 aggagtttga ccaagaacgc ttgcgttcga ggaaaaacta tctttttgac ccagtccgta
    21721 gcccgaattc agttacaaat ccgagcaaca gagtacccac tctcctttct attttctatc
    21781 tctccggata gaattaaata atcgtccgac gctgtaaggt ttagctcgat attagttaaa
    21841 ccttacggct agtgtcagag gtagcaaagg tccaatagga ctttgcgtag aaggacacat
    21901 gccttccatc tagtccgact tgctctgacc gacgtttgta cagtcaattc gataataatt
    21961 gactgtacaa actagtgcaa gggatggcct taaagtatat caccatttcc aagagcagat
    22021 gtcaacatct cagcgcagtg gttgatcggt aaatttgttc gtattctata ctccaaattt
    22081 aaccattacg actgttgcga acaaagttcg ctctatttcc aacctcaaac tgtctcccag
    22141 acagtttgag ctgtgcgggt gtgggagtga aacagtctgg ggatagactg ttttagctca
    22201 acaactggaa acaaggactt gttgacgaac tcttttttac ttttcgagtt ctttcccact
    22261 cccaggcgta gggaaatgag ttgctcgcaa ccattgacct gttccttgcc tttctattcg
    22321 tcgcctctct gagcgacatt aaataagata agtctaacaa atttctaatt gctcagttct
    22381 aaggtacctg aacagtttac ttttaagcct tgccattgac aattgcttcc aaaatcgcca
    22441 tccggacaaa tacaccattc tgcatttgac ggacaatacg tgattttggc gcctctacca
    22501 attgatcatc aatctcaaca tcgcggttaa caggggcagg atgcataaca attgcagtat
    22561 cttgcaagcg tttgtagcgc tcctctgtta aaccatgcaa acggttataa gtttctttag
    22621 aaaagccacc atcaccatca tgacgttcgt gttgtacacg gagcaacatc agtacatcta
    22681 ccgtttccac aatatcatca atattcaaat gctgaccata gacatcaaat tcctctgcgt
    22741 accattcctc cggtccagca aagaaaatct cagctcccag acgcttcaaa atttgcatat
    22801 tggacttagc aactcgtgag tgtgtaatat cacctgcaat ggcaatcttc aagccttcaa
    22861 aagttccaaa ttcttcataa atagtcatca aatcaagtag ggattggctt gggtgttgac
    22921 ctgaaccatc accaccattg acaattgaag tctgaatggt aggactatca atcaattgct
    22981 tgtaataatc cacttccgag tgacgaatca cgcagatatc taccccgagt gccgacatgg
    23041 tcaaaatggt atcatacaag gtttcaccct tattgactga gctggtacga gcatcaaaat
    23101 caatcattcc catatccaaa cgaagctctg ccatttcaaa agacttatgc gtccgagttg
    23161 aatcctcaaa gaaaaggtta gaagcatagt acttccgatc caactctacc ttttcaccgc
    23221 gtttaaaagc aataccacgt tgaatgagtc ccaatacttc ttcatttgag agggtctcca
    23281 ttgtaaccaa gtgtttgagt gaaactttac catttgtaat tgtcatgatg atacctacct
    23341 ttatgatcta gatgtagaat gagaatttgt aaacgaagcg actatgcacc ttcaaccaga
    23401 aggacagcgt cttgaccatc gatttcagcc atgtgcacaa tgatttcttc agaacgacta
    23461 gttgggatat tcttcccaac ataatctgct cgaataggca actccctgtg tccacgatcc
    23521 acaagtaccg ctaaactaac acgagctgga cggcccaagg acactatgtt gtcaatagca
    23581 gcacgaatag ttcgtcctgt ataaagcaca tcatctacta atataacatc acgatcgttg
    23641 acattggcag tcatatttgt cgtatcatct tctaccttca tgtcatcccg aaacggtttg
    23701 gtatccaact cacccaacgg aacgtcgata ccctctaatt gcttaagtct ctcctgaatc
    23761 ctctttgcaa tgaaaacacc ccgtgtttta atccctgcaa ggacaatatt atccaaattc
    23821 ttattccgtt cgataatttc ataagtgata cgggtgattg cccgtttcat agtcatatcg
    23881 tcaacgattt cttttgtctt cataagacct ccaaaagtat agaaaaagtc tccttataca
    23941 aggagactat gaaaatatag gctagttaga actaacctgc ttaacatccg actccttgtc
    24001 tgcctctctg gacaggtttt aaaggattta ttaagcagag tatagcataa aatctcagcc
    24061 ttgacaattt ttttctgaaa aaatatagaa attatcggat tttactgaga attttttcca
    24121 aaagacaatg aagtcttaag gtttatggat tttagtcccg ccaatagaaa accgcagact
    24181 agataggcga caaagatgac tccagcataa gtcaaaacat aggtccaacc atacttaggg
    24241 acattgagaa agaaataagc aaaaggactg tccttagcat ctgggattgg aatttttatg
    24301 aaaagaccat tcactagacc aaaaatcatg tagagaacag gcaaaagcgt ccaccaaatc
    24361 gggtcaaacc acttgtactg ttgctgacga tctactatta gggtatctaa caaaaaatat
    24421 aagggaacaa tataatggca gagtagattt tcaagtcgcc agaaatcgtc tgccaaaggt
    24481 gccaacataa agtgatagac tacacaggta atcatgatag acatggttac agcagccttt
    24541 atccgaagaa aacgtgacgt ctgtaaatcc acatttttac tcatggcata taccatatag
    24601 acagcgaaca gagataccaa taaattagat tgtaccgtat aatacatgag catcccgata
    24661 ccatatttaa tgatttctaa ggcggtacct gtgatggcta aaatagctaa caggcatcta
    24721 ctgtaaaaca aaatactttg atgtttcata atatcctttc aaaaacagga aaagactggt
    24781 taaccagtcc tctctcttag attttcttaa caaattcaga tttcaacttc atggcaccaa
    24841 aaccatcgat tttacaatca atattgtgat cgccttcaac gatacggatg cctttaacac
    24901 gtgtaccttg tttaaggtcc ttaggcgcac ctttcacttt caaatctttg ataagcgtca
    24961 cagtatcgcc gtccaccaaa cgattaccgt tagcatcaat tgcaataggt ccactttctt
    25021 cctcagctac atctgctggg ttccattcgt atgcacactc agggcaaacc aagagggcac
    25081 cgtcttcata gacatattca gaattacatt ttggacaatt tggtaaaatt tccattctta
    25141 ctctccttaa catttttcat gttctagtat agcatttgtt gaaaaaatgg gcaagatgat
    25201 aaggttttca
//
LOCUS       DATUCG010000017        24521 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.017, whole genome shotgun sequence.
ACCESSION   DATUCG010000017 DATUCG010000000
VERSION     DATUCG010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24521
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.017"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            327..1193
                     /locus_tag="VCA20_000564"
     CDS             327..1193
                     /locus_tag="VCA20_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP1834419.1"
                     /translation="MKWDFGTVLKEIRKSKGLSQQEVCGNALSRTTLSKIENNKEYPT
                     IAHFSHILRQLDMTFAEFDYICHAYQPSERSSIIHRFENLRGQFFSELNCQDFLYQVE
                     QYLKKQEDIRVQRIEQQIRLILSIYQTGDGERTRQLGQALWKELEKSDTWYLADFRKL
                     GLVLPIIPVSHLKGFVDKILLSLDKYKDFRDVQVSQFAFLYNLSTVLLKNEEYQLCVS
                     VMERVYAMAQESMRIDYVGMANIRMGIFQQDRGMVDKGLAILRAADLLEMVEQLEREV
                     EEYWDIFGSAEV"
     gene            1245..1457
                     /locus_tag="VCA20_000565"
     CDS             1245..1457
                     /locus_tag="VCA20_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834420.1"
                     /translation="MKITSFTELVYNRPMIGIRVYFPRSVYLRKVDQDLWLRAARFDD
                     EYASPLTTLFAFVTKNANEFVVGSEP"
     gene            1476..2462
                     /locus_tag="VCA20_000566"
     CDS             1476..2462
                     /locus_tag="VCA20_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HEP1834421.1"
                     /translation="MKELQFLIYRAENDQEKASVIVSDETIWASQKEMARLFDVGVPA
                     ISKHLKNIFEEGELDEHSVISKMETTASDGKNYLTSYYNLDAIISVGYRVNSQKATKF
                     RQWATTVLREYMIKGFAMDDERLKQGENLLEKDYFRELLERVRSIRASERRIWLQITD
                     IFAEIAIDYDANSLMTKQFYAHVQNKFHYAITGKTAAEIIYEKADHTKANMGLVTWKH
                     SPDGRILQSDAFVAKNYLNEKEIRSLERSISGYFDYLERQIEQGKTQTMKDLADSIDR
                     FLAFQDYEPLEGDGQITRQSAKEKARSEYAIFNKTQKINSDFEKLLEDLSSK"
     gene            2482..2925
                     /locus_tag="VCA20_000567"
     CDS             2482..2925
                     /locus_tag="VCA20_000567"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEP1834422.1"
                     /translation="MKIRGFELVSQFTDENLLPKRETAHAAGYDLKAAERVSLEPGEI
                     KLVPTGVKAYMQANEVLYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPANEGHIFAQM
                     RNITEETVVVEAGERIVQGVFAPFLLADGDQANGARTGGFGSTGK"
     gene            2900..3466
                     /locus_tag="VCA20_000568"
     CDS             2900..3466
                     /locus_tag="VCA20_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEP1834423.1"
                     /translation="MDLARQGNRLLCRFFFVRHSEPDYSMFDQHDNPRLYAGFGRDLA
                     PLTEKGRALAQKVASSPVFAQAQVVIASSVTRALETATYIAQAQQLPLLVEPFFHEWR
                     PDMTGQNASQDEAVLAHRLFLENSGAVPESSPVRYETAAEMRERFLQALEKYKAYDRI
                     VIVCHGMLIRQFVPKETIAYCEILEYTL"
     gene            3473..4840
                     /gene="radA"
                     /locus_tag="VCA20_000569"
     CDS             3473..4840
                     /gene="radA"
                     /locus_tag="VCA20_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEP1834424.1"
                     /translation="MIIAKKKTTFVCQSCEYHSPKYLGRCPNCGSWSSFVEEVEVAEV
                     KNERVSLTGEKTRPMKLNEVSSIQVARTKTNMEEFNRVLGGGVVPGSLVLIGGDPGIG
                     KSTLLLQVSTQLSTIGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSIRTE
                     IEKIKPDFLIIDSIQTIMSPDISSVQGSVSQVREVTNELMQIAKTNNIATFIVGHMTK
                     EGTLAGPRTLEHMVDTVLYFEGERQHTFRILRAVKNRFGSTNEIGIFEMQSQGLVEVL
                     NPSEVFLEERLDGATGSAIVVTMEGTRPILAEVQALVTPTMFGNAKRTTTGLDFNRAS
                     LIMAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVALASSYKDKPTNPQECFI
                     GEIGLTGEIRRVNRIEQRINEAAKLGFTKVYAPKNSLTGIKVSKEITVVGVTTIGEVL
                     QKVFN"
     gene            4964..5458
                     /locus_tag="VCA20_000570"
     CDS             4964..5458
                     /locus_tag="VCA20_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEP1834425.1"
                     /translation="MSYFQNFMKANKAYVDLHGDYHLPLRPKTKVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQLGTREIVVLYHTDCGAQTFTNEEF
                     AVQLKRDLEVDVAGQDFLPFTDIEESVREDIALLKQSPLIPDDVEISGAVYDVDTGRM
                     TEVI"
     gene            5666..6424
                     /locus_tag="VCA20_000571"
     CDS             5666..6424
                     /locus_tag="VCA20_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HEP1834426.1"
                     /translation="MSDNRMKYTIDSNMQFPLVEIALEAGESAYIQQGSMVYHTPSVT
                     LNTKLNARGGSGFGKLMGAIGRSMVSGESMFITQAISNSDDGKIALAPSTPGQVIALE
                     LGAEQYRLNDGAFLALDGSAQYKMERQSVGKAFFGGQGGLFVMTTEGHGTLLANAFGS
                     IKKLTLDGGSITIDNAHVVAWSRDLDYNIHMENGFLQSIGTGEGIVNTFSGYGEIYVQ
                     SLNIETFAQVIGNHIVGAGGDGGGGSTSLLDAIF"
     gene            6602..7048
                     /locus_tag="VCA20_000572"
     CDS             6602..7048
                     /locus_tag="VCA20_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator"
                     /protein_id="HEP1834427.1"
                     /translation="MKVRIEFDDSLDQVEVVIRTSQLGPEIEQIQQALQQVSRPSLVF
                     YKGSSEYFLSLGDILFFETDGTKIFAHTGDDAYEVKMKLYELEEHLPIYFCRVAKSTI
                     VNSKLVYSLNKSFSGTSRISFYKTHKEVHVSRHYYHLLKEKLQEMR"
     gene            7053..7718
                     /locus_tag="VCA20_000573"
     CDS             7053..7718
                     /locus_tag="VCA20_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834428.1"
                     /translation="MKRNGLGIALLILAGLVLFQDYLPAVEFPLWKLALVVGWSYLFL
                     KAMIKKQLVTSFVWGTLVFIVLNSHFDWLEIDTWTLILGAILACTGIKLLIKPRRSKN
                     MVDTKSFVTGVIGDKGRDTAFGSSTRYIHEENFIHDSVDVAFGSSTVYFDQAVILGNK
                     ADFHIDAAFSNITLYLPADWQAVVELDSAMSTTNNYASEPKEKVLLVTGDMAFSTLKI
                     YSM"
     gene            7810..9090
                     /locus_tag="VCA20_000574"
     CDS             7810..9090
                     /locus_tag="VCA20_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEP1834429.1"
                     /translation="MKKILKNATYLWVLTADMLSNFGDVVYYLALMNYVLLVPNSRLA
                     LAIVTFSEIFPSFMGLFTGYLADKTVNKISTIKLTLLFRVLLYLILGFCMGFEPALWI
                     VVVAATFNVFADFAGFYENGLYTPLGLRVAPKEEREQYSAFRQTVTSLLNIAFQALSA
                     LLVGLLSYQNLAFLNAGTFLAALLIMQLLTPVFRQLLTEQPLKIAEQPSQTEQKTKRP
                     GILASFKQAIVELRKIPEFRLVLITNPLINACGAILYPILVLLISEDPGLVFLNVETT
                     LALTILIFFIGHILGSTLVFTLFKNTSMVTLEVAATFSLLGVFVGMLLHQLPIIFFFL
                     TTMGISSGASGPKFNAKFVNSMPEEQLATIGGAVSTYFMLGQAFTRLLVSGLVLVLAV
                     DQISGLFLTTTGLLVLYVLYWLARNQKTPQNQPN"
     gene            9163..10617
                     /locus_tag="VCA20_000575"
     CDS             9163..10617
                     /locus_tag="VCA20_000575"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEP1834430.1"
                     /translation="MTKPIRVRYAPSPTGLLHIGNARTALFNYLFARHHGGTFLIRIE
                     DTDRKRHVEDGERSQLENLRWLGIDWDESPETHENYRQSERLDIYQKYVDQLLAEGKA
                     YKSYVTEEELAAERERQEAAGETPRYINEYLGMSEDEKAAYIAEREAAGIVPTVRLAV
                     NETGIYKWNDMVKGEIEFEGGNIGGDWVIQKRDGYPTYNFAVVVDDYLMKISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRRKGYM
                     PEAVFNFIGLLGWNPGGEEEIFSREQFIQLFDENRLSKSPAAFDQKKMDWMSNEYIKN
                     ADLETIFNLAKPFLEEAGRLTDKAEKLVELYKPQMSSADEIVGLTDLFFSDFPELTDE
                     EKEVMAGETVPTVLNAFKEKLEAMTDGDFQPDNIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPNTIYLLGREKSIQHIENMLKSL"
     gene            complement(10658..11011)
                     /locus_tag="VCA20_000576"
     CDS             complement(10658..11011)
                     /locus_tag="VCA20_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEP1834431.1"
                     /translation="MKTGQVMQQFGIKRDTLRFYTEQGLLQPQLIDGNYYWNEEEINN
                     LENILGLRQLGLSVKAIIRIKELHDTKCGSLEQLKENKQVILDEIADREKQILLLQEQ
                     KENLLQQIEEKLQDL"
     gene            11071..11361
                     /locus_tag="VCA20_000577"
     CDS             11071..11361
                     /locus_tag="VCA20_000577"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834432.1"
                     /translation="MIIVVKWLIVLYLGLLPLSAWVYRKQLPNWLLALYACPVLLSLL
                     SWQAVWGCILGLILACGVRVIGGRVLFGKNRLSHFLAHFLLSLVLALALFLV"
     gene            complement(11320..11850)
                     /locus_tag="VCA20_000578"
     CDS             complement(11320..11850)
                     /locus_tag="VCA20_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEP1834433.1"
                     /translation="MEIWNAYTADGHLTDHTLTRGESIPNDLYHLVVECIIRHHDGST
                     LFMKRDSTKPSYPDYYEATAGGSALFGEIAEQAILREVREETGIELTADQLRHHTHFV
                     AHDDQCIFHCYWAETDWDKSTIQLQANETSHYIWVPQENLKDFLETELVIPRQKDYVE
                     RLFLDQEKCKSENQAQ"
     gene            11971..12846
                     /locus_tag="VCA20_000579"
     CDS             11971..12846
                     /locus_tag="VCA20_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEP1834434.1"
                     /translation="MQTIIPHLWYDTEAKEAVAFYVELFGGKIDWTYTITDTPSGDSD
                     LIQFQLGDMTLAAISAGPYFKLNESMSLMVNVASKDEVTRLYQALSEGGRILMPLGEY
                     PFSPYYVWLEDRFGLSWQLSYAPDLDKPYQFDICLLFSQEQVGLAQHMLDYYKDKLPQ
                     ARLGRLSYYGEGEAAVAAAKLNYAELLVAGQKMIVMDHGYGGVASFNEAFSFMVYVDS
                     QEEAESWYEEVSAVPEAEICGWAKDQFGISWQIVPRILMEAYDTASPEKVKAVNAAVM
                     TMKRLDIAAIQALLN"
     gene            12953..13477
                     /locus_tag="VCA20_000580"
     CDS             12953..13477
                     /locus_tag="VCA20_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEP1834435.1"
                     /translation="MKATERRKKILSLLRESLDPLAANYLAKEFGVSRQVIVGDVALL
                     RAEGEAVLATPRGYLLQSSLPSTEGAYIRKIACQHGPEEVEKELTILLEGGAQILDVE
                     IEHPIYGLLSGKLNIASLEDRDHFLDALGQYQGVLLSNLTEGVHTHTVSFPSVQAYNE
                     LSAALKHAGILLEN"
     gene            13550..14098
                     /locus_tag="VCA20_000581"
     CDS             13550..14098
                     /locus_tag="VCA20_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834436.1"
                     /translation="MSQLTLSSKNSVKQLSISAILTAFAILIPLMMPIKIIIGPASYT
                     LASHVPLFMAMFVSPATAIFVALGSSLGFFLAGFPIVIVFRALTHLFFLTLGAVLVKR
                     FPTLMDSKRSLLLGIGLNLLHGLGEYIVVMVLTSGQQTSATYWITMLGLVGVGSAIHG
                     LLDFSLAYYFWKILKERKIYQP"
     gene            14291..15484
                     /locus_tag="VCA20_000582"
     CDS             14291..15484
                     /locus_tag="VCA20_000582"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEP1834437.1"
                     /translation="MTKEKLILAYSGGLDTSVAIAWLKKDYDVIAVCMDVGEGKDLDF
                     IHDKALSIGAIESHVLEVKEEFAQEYVLPALQAHAYYEQKYPLVSALSRPLISKKLVE
                     MAHKTGATTIAHGCTGKGNDQVRFEVAIAALDPSLKVVAPVREWKWAREEEIIFAKEN
                     GVPVPADLDSPYSVDQNLWGRANECGVLENPWNEAPEDAFGITTSPENAPDTPEYVDI
                     EFKAGIPVAVNGEKLSLANLIQRLNVIAGKHGVGRIDHVENRLVGIKSREIYECPGAI
                     TLLTAHKEIEDLTLVREVSHFKPIVSNELSNLIYNGLWFNPATDALKAYLAQTQAVVN
                     GAAKVKLYKGSAKVVARKSPNSLYDEDLATYTSADTFDQDAAVGFIKLWGLPTKVNAE
                     IHKKS"
     gene            15498..16892
                     /gene="argH"
                     /locus_tag="VCA20_000583"
     CDS             15498..16892
                     /gene="argH"
                     /locus_tag="VCA20_000583"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEP1834438.1"
                     /translation="MEAKKLWGGRFEASLEEWVEEFGASIRFDQKLAKYDIQGSLAHV
                     KMLGQTGIISQAEAQVIQAGLEELLEEYEAGKLVFDIRNEDIHMNIESLLTEKIGSVA
                     GKLHTARSRNDQVATDMHLYLKDTLFQVVDKLTNLRQILVDLAQEHVETIMPGYTHLQ
                     HAQPISFAQHLLAYYNMFSRDSERFAFNMQHTNVSPLGAAALAGTTFPIDRQMTSDLM
                     GFAQPYSNSLDAVSDRDFILEFLSNASILMMHMSRLCEEIINWCSHEYQFVTLSDTFS
                     TGSSIMPQKKNPDMAELIRGKTGRVYGNLVGLLTVMKSLPLTYNKDLQEDKEGMFDTA
                     ETVLISIDILAGMLQSMTVHKERMAQLTEKDFSNATELADYLASKGLPFRQAHEIVGK
                     LVLECSKAGHCLQDVSFETYQAISPLIQADIYDALSSKVAVSRRNSLGGTGFESIADQ
                     LKSAKEEIQNAKSL"
     gene            16963..17322
                     /gene="rnpA"
                     /locus_tag="VCA20_000584"
     CDS             16963..17322
                     /gene="rnpA"
                     /locus_tag="VCA20_000584"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEP1834439.1"
                     /translation="MKKSYRVKRERDFNDIFTKGNNVANRRFVVYRLPKEQKHFRVGL
                     SVSKKLGNAVMRNKIKRRIRHILIECQHELVADDFVIIARKGVEELSYQEMKQNLIHV
                     LKLAKLYQEGVVIEKES"
     gene            17306..18118
                     /locus_tag="VCA20_000585"
     CDS             17306..18118
                     /locus_tag="VCA20_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002267318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEP1834440.1"
                     /translation="MKKKVKWAGLLVASLIVLTACGTGPVTSQSTDAWDRLIYWFASI
                     IQFLSINGQIGIGIILFTILIRTLLLPLFQIQMNSSRKMQELQPQLKKLQAEYPGTDM
                     ESRQQLYEATQALYKENGVSMRASMIPLFIQMPILLALFQALTRVEALKVGHFLWLNL
                     GEPDPFFILPILAAVFTFLSSWLTNKSAIEKNMAMTIMTYVMPVMIFFFAVAAASGVA
                     LYWTVSNAYQVAQTLVFNNPFKIIAERQAKIDAEKERRAKIRRAQKKAQKKK"
     gene            18138..19133
                     /locus_tag="VCA20_000586"
     CDS             18138..19133
                     /locus_tag="VCA20_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HEP1834441.1"
                     /translation="MKFTGASVEEAIQNGLVELNIPRKKAHITVVAREKKGFLGFGKK
                     PAIVDIDVINETTVVKANQKAVRGVPSEINDLNEPVKSVSEATIDLGKVVAAVKEFEK
                     SGQSLDDDIKAQILKNEKEATTILEETGRIEILSEDLLTTTDATTSAKEDVNQEVISE
                     NQADNFADLEIKIEPQYDIEQVVSEVTAYVQEILDDMDVEARIDTSHNRRTINIQVDT
                     NEPGRVIGYHGKVLKALQLLAQNFLYNRYERNFYITINVNDYVEHRAEVLQGYAQKLA
                     ERVLAEQEAYHTDPMSSSERKIIHRIISKMDGLTSYSEGSEPNRYVVVDIEGNHD"
     gene            19301..19438
                     /gene="rpmH"
                     /locus_tag="VCA20_000587"
     CDS             19301..19438
                     /gene="rpmH"
                     /locus_tag="VCA20_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEP1834442.1"
                     /translation="MKRTFQPSKIRRARKHGFRSRMATKNGRRVLAARRRKGRKVLTV
                     A"
     gene            19545..20327
                     /locus_tag="VCA20_000588"
     CDS             19545..20327
                     /locus_tag="VCA20_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEP1834443.1"
                     /translation="MIQIFDTHTHLNVEQFDGRVQEELDFAKEMGVTLHNVVGFDRVT
                     IDKAMELADQHPEIYLTLGWHPTEAGTYDEEVENYLLKALQHPKVIALGEIGLDYYWM
                     TADKETQARVFKRQMELAKQLDLPFVVHTRDALEDTYQIIKEVGVGPRGGIMHSFSGT
                     LEEAQAFMDLGMHISFSGVVTFKKAVELQEAAQALPLEKILVETDAPYLAPVPKRGRE
                     NRTGYTRYVVDMLAQLRNQSVEEVARATCENARSLFRIDSEN"
     gene            20290..20880
                     /gene="rnmV"
                     /locus_tag="VCA20_000589"
     CDS             20290..20880
                     /gene="rnmV"
                     /locus_tag="VCA20_000589"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEP1834444.1"
                     /translation="MQGVCSELTQKIKIQEVLVVEGKDDTANLKRFYEVDTYETRGSA
                     ISDDDLERIEKLHDLRGVIVFTDPDYNGERIRKIIMQAIPTVKHAFLQRDEAAPKSKT
                     KGRSLGIEHASFEDLEKALAGVRGYYDDEHNFDITKSDLMRLGLLMGGDSRKRREYLG
                     EKLRIGYCNGKQLLKRLELFGISLAEVEEAMGSYHG"
     gene            20873..21241
                     /locus_tag="VCA20_000590"
     CDS             20873..21241
                     /locus_tag="VCA20_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834445.1"
                     /translation="MDRVYAVKCLVKNEFEPSYENYLSYEEVTYLLPADVLVWKDEQV
                     DKNKLADYLCGLVEDGYVNADGIWKQSRLFHIAGVQEMDDRVEFDGPAVEVYRWFYNF
                     ERPVTEEEFLNLYYFDDLGR"
     gene            21339..21626
                     /locus_tag="VCA20_000591"
     CDS             21339..21626
                     /locus_tag="VCA20_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin subunit"
                     /protein_id="HEP1834446.1"
                     /translation="MITIKKDAQVNFKTDSRLLEKAKEVFAMNQLDLTAGFNLFLQNI
                     AVKNELPILTEEELEREELFLQLQKEIQENQQAIEAGHGISLESVKEKYGI"
     gene            21616..21945
                     /locus_tag="VCA20_000592"
     CDS             21616..21945
                     /locus_tag="VCA20_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEP1834447.1"
                     /translation="MEFESYSVILAPAVEKELAVIYAYFSEQFSEEIAKRRIGMIVEA
                     LESLQIFPERGFNADNRFGKQIDPPHLTRGYVVGKDYIALYRVVKNEVRVGHLFATKS
                     DYVKLLK"
     gene            21952..22407
                     /locus_tag="VCA20_000593"
     CDS             21952..22407
                     /locus_tag="VCA20_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEP1834448.1"
                     /translation="MSRTEQVILTNMCMITDGQQVLVQDRKSKKWSGVTFPGGHVEPN
                     ESIISSVIREIKEETGLTVSNLELCGIQNWTDPTDHYRYLVFCYKTSQFSGVPQSSDE
                     GEVFWIDRAELKNVQLADGFETMLEIFEQPQLMENFYWFEDGEWKMENR"
     gene            22483..22743
                     /locus_tag="VCA20_000594"
     CDS             22483..22743
                     /locus_tag="VCA20_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEP1834449.1"
                     /translation="MEAIVYSHFRNHLKDYMKKVNDEFEPLVVVNKNPEEDIVVLSKS
                     EWDSLQETLAVARNTYLSQKVLRGMAQVKAGQTQERNLIEAD"
     gene            22745..23002
                     /locus_tag="VCA20_000595"
     CDS             22745..23002
                     /locus_tag="VCA20_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEP1834450.1"
                     /translation="MGIHFTEDAWEDYLYWQGQDKKTLKRINKIIKDMQRHPFEGIAK
                     PEPLKYDYQGAWSRRIDAENRLIYMMEADRLCILSLKDHYR"
     gene            23016..23546
                     /locus_tag="VCA20_000596"
     CDS             23016..23546
                     /locus_tag="VCA20_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEP1834451.1"
                     /translation="MKKVVLLRGVTPVGKNKIPKMSYLAEILTEVGLTSVQTYIQSGN
                     VVCETDILDEELSRLIHQTIKEKIGAELAIIVKHQMELTQAVAENPFGEGYDPSRVHL
                     VFTNDEIAGEKLNKLLHQDFGDEELRLGSQCLYMYLPRDARKKKLNTNYLEKQLDITA
                     TMRKLSVISRLSEMAK"
     gene            23530..24438
                     /gene="rsmA"
                     /locus_tag="VCA20_000597"
     CDS             23530..24438
                     /gene="rsmA"
                     /locus_tag="VCA20_000597"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEP1834452.1"
                     /translation="MKWRNKKQERTHMRIADKNVTRAVLERHGFTFKKSFGQNFLTDT
                     NILQKIVDTAEIDQNVNVIEIGPGIGALTEFLAESAAEVMAFEIDDRLIPILADTLGH
                     FDNVTVVNQDILKTDLQTQIQNFKNPDLPIKVVANLPYYITTPILMHLIESKIPFSEF
                     VVMMQREVADRISAEPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKM
                     VRREQPLVAVRDEDFFFRVSKASFVHRRKTLWNNLTNHFGKSDEIKDKLTQALGMAEL
                     EASVRGEALSMADFARLSDRLQEVGL"
BASE COUNT         6815 a         4881 c         5929 g         6896 t
ORIGIN      
        1 agtgtatttt tattttcaag attagctttc ccatcctctc cacccaacat atagtttcca
       61 gtcattttcc tcctttcccg agtggtgaga tggtagtagc ttatttattt gttgacaatt
      121 ggtataatta gattccatcc atctcctaaa agttgagggg ctagtaggat ggctgagtaa
      181 gttaaaagca agagatagtt tttccaattt ttcataagag tgcctcctat tattttgttt
      241 gtttttcttt actattatgg tatcatagaa taaatgcaat ttatgaaaac gcaaacaatt
      301 ctcacatata ttctgggagg tcttgcatga aatgggattt tggaacagtc ttaaaagaaa
      361 ttcgcaagtc aaaggggctt agccagcagg aagtgtgtgg caatgccttg tcacggacga
      421 ccctatcaaa aattgaaaac aacaaggagt accctaccat tgcccacttt tcccatatcc
      481 tccgtcagct ggatatgacc ttcgcagagt tcgactatat ttgccatgcc tatcagcctt
      541 cggagcgttc gtctatcatc catcgctttg aaaatcttag aggacaattt ttttctgaat
      601 taaactgtca agattttttg taccaagtag agcagtattt gaagaagcag gaagatatac
      661 gggttcagcg aatcgaacaa caaatacgct taatcttatc catttatcaa acgggagatg
      721 gggagcggac cagacagttg gggcaagcac tttggaagga attggaaaag tcggatactt
      781 ggtatttagc tgattttcgt aagttggggt tggtacttcc tatcattcct gtatcccatt
      841 tgaaaggctt tgtagataaa atcttgcttt ccttggacaa gtataaggac ttcagagatg
      901 tacaggttag tcagtttgct tttctttaca atctatcgac tgttttgttg aaaaatgaag
      961 agtatcaact ttgtgtttca gtaatggaga gagtttatgc catggctcag gaaagtatgc
     1021 ggattgatta tgtaggaatg gccaatatcc gtatgggcat ctttcagcag gatagaggga
     1081 tggtagataa gggacttgcc attctccgag ccgcagactt gctagagatg gttgaacagt
     1141 tggagagaga agtcgaggag tattgggata tttttggctc ggcagaggtt tgaactgctt
     1201 gagctagttt atagtagtag cacctgtttg tcaggtgttt tttgctgaaa atcacgagct
     1261 ttaccgaatt agtgtataat agacctatga ttggaatacg agtttacttc cctcgcagtg
     1321 tctatctccg taaggttgac caagacttgt ggctacgtgc ggctcgcttt gatgacgagt
     1381 acgcctctcc cttaacaaca ctttttgcat ttgtgacgaa aaatgcgaat gaatttgtag
     1441 tggggagtga gccctaaata attaaagagg caaatatgaa ggaattacaa tttttaattt
     1501 accgagcaga aaatgaccaa gaaaaggcta gtgttattgt cagtgacgag acgatttggg
     1561 ctagtcagaa ggaaatggca agattatttg atgttggtgt gccagctatc agcaaacatt
     1621 tgaaaaatat ttttgaagaa ggtgaattag atgagcattc agttatttcc aaaatggaaa
     1681 caactgcaag tgatggtaaa aattatctaa ctagctatta caatctcgat gccatcattt
     1741 ccgtcggtta ccgtgtcaat tctcaaaagg ctactaagtt taggcagtgg gcaaccactg
     1801 tgcttcgaga atacatgatt aaaggttttg ccatggatga tgagcgtttg aagcaaggcg
     1861 aaaacttgtt ggaaaaagac tatttccgtg agcttttaga acgagttcgt tctatccgtg
     1921 ctagtgaacg acgaatctgg ttacaaatca cagatatttt tgcagagatt gcgattgatt
     1981 atgacgctaa tagcttgatg actaagcaat tctatgctca tgtgcaaaat aaatttcatt
     2041 atgcgattac aggtaaaaca gctgcagaaa ttatttatga aaaggcagac cacactaaag
     2101 cgaacatggg gttggtaacc tggaagcact cccctgacgg acgtattttg caatcggatg
     2161 cttttgttgc taaaaattat ctgaacgaaa aggagattcg ttcgctggaa cgtagtattt
     2221 cgggctactt cgattatctg gagcgacaaa ttgagcaagg aaagactcag accatgaaag
     2281 atttggcaga tagcattgat cgatttttag cctttcaaga ttatgaaccc ttagaggggg
     2341 atggtcaaat tacaaggcag tcagccaagg agaaagctcg gtcagaatat gcaattttta
     2401 ataagaccca aaagataaat tctgattttg aaaaattact agaagattta tcttcaaaat
     2461 aagtactcga aaggaaaaaa catgaaaatc cgtggattcg aactggttag ccagtttact
     2521 gatgaaaatt tattgccaaa acgtgagacc gctcatgcag caggttacga cctaaaggct
     2581 gcggagaggg tcagcttgga gccaggtgag attaagctgg ttccaacagg tgtaaaggcc
     2641 tatatgcagg ccaacgaggt cctatatctt tacgaccgtt cgtccaaccc tcgcaagaag
     2701 gggttggttt tgattaactc ggttggggtt atcgacggtg actactacgg caatccagcc
     2761 aatgaaggcc acatctttgc ccagatgcga aatatcacag aggaaaccgt cgtggtggaa
     2821 gcaggcgagc ggatcgtgca gggtgttttt gcccccttcc tcttggctga cggcgaccag
     2881 gccaacggcg ctcggacagg tggatttggc tcgacaggga aatagactac tatgcagatt
     2941 cttttttgtg cggcattccg agcccgacta cagtatgttt gaccagcatg ataatcctcg
     3001 gctgtacgca ggttttggac gggatctggc tcccctgact gaaaagggaa gggctttggc
     3061 ccagaaagtt gccagtagtc ctgtttttgc tcaggcccag gtggttatcg cttcaagcgt
     3121 cacgcgggct ttggaaactg cgacctacat tgctcaagcc cagcaactac ctcttttggt
     3181 cgagcccttt tttcatgagt ggcgaccaga tatgacgggg caaaatgcca gtcaagatga
     3241 ggctgtctta gctcatcgat tatttttgga aaatagcgga gcggtgcctg agtcttcacc
     3301 tgttcgctat gagactgctg cggagatgag agagcgattt ttacaggccc ttgaaaaata
     3361 caaagcctat gaccgaatcg ttatcgtctg tcacggtatg ctgatacgcc agtttgttcc
     3421 caaagaaact atagcctact gcgaaatcct agaatacact ctataggagg aaatcatcat
     3481 cgctaagaaa aaaacaacct ttgtctgtca atcctgcgag taccactcgc ctaagtatct
     3541 gggccgttgc cccaactgtg gctcctggtc tagctttgtc gaggaagtgg aagtcgctga
     3601 agttaagaac gagcgggtca gcctgacagg tgagaagacc cgccccatga agctcaatga
     3661 agtgtcgtcc attcaagtgg ctcgcaccaa gaccaatatg gaagagttta accgcgtcct
     3721 tggtggtgga gtggtgccgg gaagtctagt tctcattgga ggcgatccag ggattggcaa
     3781 gtccaccttg ctcctgcaag tatcgaccca actttcgact attggcaccg ttctctacgt
     3841 gtcgggggag gagtctgccc agcagattaa gttgcgagcg gaacgcttgg gcgacattga
     3901 cagcgagttc tatctctatg cggagaccaa tatgcagagc attcggaccg agattgagaa
     3961 aattaagcca gatttcctga ttatcgattc gatccagacc attatgagcc ctgacatctc
     4021 cagcgtgcaa ggctctgtca gtcaggtccg tgaagtgacc aatgagctca tgcagattgc
     4081 caagaccaac aacatcgcaa cctttatcgt cggccacatg accaaggaag gcaccttggc
     4141 tggaccgcgg accttggagc atatggtaga taccgttctc tattttgagg gcgagcggca
     4201 gcacaccttc cgtatcttgc gggcggtcaa aaaccgcttc ggctctacca acgaaatcgg
     4261 catttttgaa atgcagtcac agggcttggt cgaagttctc aatccaagtg aggtctttct
     4321 ggaagagcgt ctggacggag cgactggctc tgccattgtc gtgactatgg agggcacccg
     4381 ccccatcctt gcggaagtgc aggctttggt gacgccgacc atgtttggca atgccaagcg
     4441 gaccacgaca ggcttggatt tcaaccgtgc cagcttgatt atggcggttt tggaaaaacg
     4501 ggcaggcctg ctcctccaaa accaagacgc ctaccttaag tcagcaggcg gtgtcaaact
     4561 ggatgagcca gctattgacc tagcggtcgc agttgcccta gcttccagtt acaaggataa
     4621 gccaaccaac ccacaagagt gctttatagg cgaaatcggt ctgacagggg aaatccgccg
     4681 cgtcaatcgg attgaacaac ggattaacga agccgccaaa ttgggcttta ccaaggtcta
     4741 tgcccccaaa aattccctga caggtatcaa ggtgtccaag gaaatcacgg ttgttggcgt
     4801 gacaaccatt ggcgaagtct tacagaaagt attcaactga tgaaatcatt cgggagtgac
     4861 agctagtcgc tcctcttttt gcgacaaaag ttaagaaaca tggtaatcta ggtgtaacct
     4921 atttctgaaa aattcagaaa ttcttatcag acaaggaggt cctatgtctt attttcaaaa
     4981 ttttatgaag gccaacaagg cctatgttga cctgcatggt gattaccatt tgcctcttcg
     5041 tccaaagacc aaggtggcta tcgtgacttg tatggactca cggcttcacg tggcgcaggc
     5101 tttgggattg gctcttggtg atgcccacat tttgcggaat gcgggtgggc gcgtgacgga
     5161 ggacatgatt cgttcgttgg tcatttccca gcagcagctc ggtacgcggg aaatcgtggt
     5221 gctctaccat acagactgtg gtgcccagac ctttaccaat gaagaatttg cagtccaact
     5281 caagcgagat ttggaagtgg atgtggctgg tcaggacttt cttcctttta cagacattga
     5341 agaaagcgtg cgggaggata ttgctctctt gaaacaatct ccgcttatac ctgacgacgt
     5401 tgaaatttct ggtgcggttt atgatgtgga tacgggtcgt atgacagaag ttatatagga
     5461 atactcagga agttcggttt ttcctgtaaa ttttatctga atggccgaga aatacttgac
     5521 aagttttttt gaatgagata aaattgtatt atcacaacag aacaaacaaa gagataaatg
     5581 atacttggtt tattttagct tgttcttggt ttattatggc tatcctgtgg ttaacaggac
     5641 ctttcgtaaa aaataggagt caactatgtc agacaatcgg atgaaatata ctatcgatag
     5701 taatatgcag tttcctctgg tggaaatcgc cttggaagct ggtgaatcag cttatattca
     5761 acaagggagc atggtctacc atacgccgag cgtgaccttg aataccaagc tcaatgcgcg
     5821 tggtggttca ggttttggta aattgatggg ggccatcggt cggtcaatgg tatcaggcga
     5881 atctatgttt atcacgcaag ccatttccaa tagtgacgat ggaaaaattg cccttgcgcc
     5941 ttcaacacca ggtcaggtta ttgccttgga acttggtgca gagcaatatc gactaaatga
     6001 tggtgccttc cttgcccttg acggttctgc ccagtataaa atggaacgcc agagtgttgg
     6061 gaaagctttc tttggtggtc agggtggact ctttgtgatg actacagagg ggcatggcac
     6121 acttttggcg aatgcgtttg gttctattaa gaagctaact ttggatggtg gaagtatcac
     6181 catcgacaat gctcatgtgg ttgcttggag tcgtgatttg gactacaata tccatatgga
     6241 gaatggtttc ctccaatcta tcggtactgg ggaaggaatt gttaatacct tctctggtta
     6301 tggagaaatc tatgtacaga gtttgaacat tgagaccttt gctcaggtca ttggcaatca
     6361 tattgtcggt gcaggtggtg acggtggtgg tggaagcaca tcattgctgg atgcgatttt
     6421 ttaagaagaa aaactctgca attttcaaaa tcatataata aaaataaagt ccctcgcgta
     6481 atgcaaggga cttttcttag tttatttcta ccacttacta gcacattttt accacttact
     6541 gaaatgccct tgtaattgga tgtcgaactg gtataataga gctatcaaaa aggagggagc
     6601 tatgaaagtt aggattgaat ttgatgacag cttagatcag gttgaagttg ttatccgaac
     6661 cagtcagctg ggaccagaga ttgaacagat tcagcaagct ttgcaacaag ttagtcgtcc
     6721 ctcgcttgtt ttttacaagg ggagcagcga gtattttctt tcattggggg acatactttt
     6781 ctttgaaacg gatggaacca agatttttgc tcatacaggc gatgatgcct acgaggttaa
     6841 gatgaaactc tatgaacttg aggagcattt gcccatctac ttttgtaggg tagctaagtc
     6901 aaccattgtc aatagcaagt tagtctattc gctaaataag tccttctcgg gcaccagtcg
     6961 gatttccttc tataagactc acaaggaagt ccacgtgtcg cggcattatt accatttatt
     7021 aaaagaaaag ttacaggaaa tgaggtagga agatgaaaag aaatggtttg ggaatagctt
     7081 tactgatttt ggcaggcttg gtcttgtttc aagactactt gccggctgtg gaatttcctc
     7141 tttggaagtt ggcactggta gtcggttgga gttatctttt tttaaaagca atgataaaaa
     7201 aacagctagt tacaagcttt gtctggggaa ccttggtctt tattgtgcta aacagtcact
     7261 ttgattggtt ggaaattgat acctggacct tgattttggg tgcgatactg gcttgtacag
     7321 ggataaagtt gttaataaaa cctaggagga gcaagaatat ggtcgataca aaaagttttg
     7381 tcacaggggt gattggcgac aaggggcgcg atacagcttt tggttcctcg actcgttata
     7441 tccatgagga gaattttatt catgatagtg tggatgtagc ctttggatct agtacagtct
     7501 attttgatca ggcggtgatt ctgggtaata aggcagattt tcatatcgat gcggccttct
     7561 caaatatcac actctatctg cctgctgact ggcaagcggt tgtagagttg gatagcgcca
     7621 tgtcaacaac taacaactac gcatctgagc caaaagaaaa ggttctcttg gtgacgggtg
     7681 atatggcatt ttctacatta aaaatttact ctatgtaaaa gaataatcgc agaggaaagt
     7741 ctatccaggc tcctctgtaa aataaaaatt cggaatattc tggaaattat tcaaaagaaa
     7801 gggattatca tgaaaaagat tttgaaaaat gctacttacc tatgggtact gactgcggat
     7861 atgctgtcca attttggcga tgtcgtctat tatctggcct tgatgaacta tgttttgctg
     7921 gtgcccaaca gtcgtctggc tctggcgatt gtgaccttct cggaaatctt ccctagcttt
     7981 atgggactct ttacaggcta tctggcagac aagacggtca acaaaatcag tactatcaag
     8041 ctgaccctgc tctttcgtgt ccttctctac ctcatcctcg gcttctgcat gggctttgag
     8101 ccagccctct ggattgtcgt ggtggctgca actttcaatg tctttgcaga cttcgctggc
     8161 ttctatgaaa acggcctcta tacacctctg ggattacgcg tagcgcccaa agaagagcgg
     8221 gagcagtact cggcctttcg ccagaccgtg accagcctcc tcaatatcgc ctttcaggcc
     8281 ctctctgccc tcttggtcgg cctcctctcc taccaaaacc tggcctttct caatgccggc
     8341 acctttttag ccgccctcct catcatgcag ctgctgacgc cagtctttcg ccaactcctg
     8401 acggagcagc ctttgaaaat agcagagcag ccgagccaga ctgagcaaaa aaccaagaga
     8461 ccaggcattc tagcttcctt caagcaagcc atcgtggagc tgcgcaagat acccgagttc
     8521 cgcctggtcc tgattaccaa ccctcttatc aacgcctgtg gagcgattct ctatcctatt
     8581 ctggtcctgc tgattagcga agacccaggc ctcgtcttcc tcaatgttga aacaaccctt
     8641 gccctgacca ttctgatctt ctttatcgga catattctgg gctcgacctt agtctttacc
     8701 cttttcaaaa ataccagcat ggtgacgctg gaagtggcag ccaccttctc tcttcttggc
     8761 gtgtttgtcg gaatgttgct acatcaactg ccaattattt tcttcttcct tactacaatg
     8821 gggattagtt caggagctag tggacctaag tttaacgcta agtttgtcaa ttctatgcca
     8881 gaggagcagt tggcgaccat tggcggagcg gtatcaacct attttatgct aggtcaagcc
     8941 tttactcgac tgttggtttc gggtctggtc ttggtattgg ccgtcgatca gattagcggt
     9001 cttttcctaa cgacaactgg tttgttagtc ctctatgtcc tctactggct ggcacgcaac
     9061 caaaaaacac ctcaaaatca gcccaactag gctgattttt ctgttttctt actcgtatct
     9121 atgctataat gttcttgatt gaaattttac agggagagaa acatgaccaa accaattcgc
     9181 gtgcgttacg caccaagtcc aacaggactc ttgcatatcg gaaatgctcg tacagcattg
     9241 tttaactatc tttttgcccg tcatcacggc ggtactttcc ttattcgcat cgaggacaca
     9301 gaccgcaaac gccacgtaga agacggtgag cgttctcaac ttgaaaacct gcgttggttg
     9361 ggtattgact gggatgagag tccagaaacc catgagaact accgtcagtc tgagcgtttg
     9421 gatatttacc aaaagtatgt tgatcagctc ttggcagaag gcaaggccta caagtcttat
     9481 gtgaccgaag aagagttggc ggcggagcgt gagcgtcagg aagctgcggg tgaaactcct
     9541 cgctacatca acgaatacct tggtatgtct gaagatgaaa aagcggccta catcgcagag
     9601 cgtgaggcgg ctggcattgt cccaacggtt cgcttggctg tcaacgaaac aggtatctac
     9661 aagtggaatg acatggttaa gggcgaaatc gagttcgaag gtggcaacat cggtggtgac
     9721 tgggttatcc agaaacgtga cggctatcca acttacaact ttgctgtggt ggtggatgat
     9781 tatctcatga aaatctcgca cgttatccgc ggggacgacc acattgccaa tacacctaag
     9841 caattgatgg tctatgaagc acttggttgg gaagcaccag aatttggtca catgactttg
     9901 attatcaact ctgagactgg taaaaaactg tctaaacgtg atactaacac ccttcagttc
     9961 atagaagatt accgtcgtaa aggttacatg cctgaagctg ttttcaactt tatcggtctt
    10021 ttgggttgga acccgggtgg tgaagaggaa atcttctctc gtgagcaatt tatccaactc
    10081 tttgacgaaa atcgtctcag caagtcacca gcagcttttg accagaagaa aatggactgg
    10141 atgagcaatg agtacatcaa aaatgctgat ttggaaacta ttttcaacct tgctaaacca
    10201 ttcttggaag aggctggtcg cttgacagac aaggcagaaa aattggtgga actttacaag
    10261 ccacagatgt catcagcaga cgaaattgtt ggtttgacag acctcttctt ctctgacttc
    10321 cctgaattga cggatgagga aaaagaagtc atggcgggag aaactgttcc gactgtttta
    10381 aatgccttca aggaaaaatt agaagccatg acagacggag atttccaacc agataacatc
    10441 ttcccacaaa tcaaggctgt tcaaaaagaa acaggtatca agggcaaaaa cctcttcatg
    10501 ccaatccgta tcgccgtttc gggcgaaatg cacggaccag agttgccaaa tactatttac
    10561 ctgctcggtc gtgaaaaatc aatccagcat attgagaata tgttgaaatc tttgtaagac
    10621 aagtaatccc cgatgtcctg tgacagcggg gattttgcta taaatcctgt aatttttcct
    10681 caatctgctg gagtagattt tccttctgtt cttggagcag gaggatttgt ttttcacggt
    10741 cggcaatttc atccagtatg acctgtttgt tttcttttag ttgttcgaga ctgccgcatt
    10801 tggtgtcatg tagttccttg atgcggataa tggctttgac ggataaacct aattggcgaa
    10861 gtcctaggat attttctagg ttgttgattt cttcttcgtt ccagtagtaa ttgccgtcaa
    10921 tcaattgcgg ttgtagcagt ccttgttctg tgtagaaacg aagcgtatcc cgtttgatac
    10981 cgaattgttg catgacttgt cctgttttca taatgtcctc ttgacagggg cttagccctc
    11041 atgataaatt ggtggggaaa ggagttctat atgattattg ttgttaaatg gttgattgtg
    11101 ctttatttgg ggctattacc gctatctgct tgggtttatc gcaagcaact gcccaattgg
    11161 ttattagctt tatatgcctg tccagtgttg ctcagtttgt tgagctggca ggcggtttgg
    11221 ggatgtatct tgggtctaat cttggcttgc ggggtgagag tgataggtgg tcgtgttctt
    11281 tttggaaaaa atcgtcttag ccacttctta gctcattttc tactgagctt ggttctcgct
    11341 cttgcacttt tcttggtcta aaaacaacct ctccacatag tctttctgcc ttgggataac
    11401 cagttctgtt tcaagaaaat ctttcaaatt ttcctgtggg acccagatgt agtggctggt
    11461 ttcgttggct tggagttgga tagtagattt gtcccagtcg gtttcagccc agtagcagtg
    11521 gaaaatgcac tggtcgtcat gggcgacaaa gtgtgtatgg tggcggagtt ggtcagctgt
    11581 cagctcaatg cctgtctctt cgcggacttc gcgcaaaatg gcctgctctg ctatttcccc
    11641 aaacaaagct gaacctcctg ctgtcgcttc ataatagtca gggtaggagg gcttggtgct
    11701 gtcgcgtttc atgaagaggg tgctgccgtc gtggtggcgg atgatgcact cgacgactag
    11761 gtggtagagg tcattgggaa tggattctcc gcgtgtgagg gtgtggtcgg tcaagtgacc
    11821 gtcggctgtg taggcgttcc agatttccat ttcattctcc tgtcaatttt gtagttattc
    11881 agtatagcac atctgtaagt gtaaagcaac atgctttgtt tttgtacgga caagtgttat
    11941 actggggcta ctaactcaaa ggagaaatca atgcaaacca ttatcccaca tctctggtat
    12001 gatacagagg caaaagaagc ggtagctttc tatgtagaac tcttcggagg caagattgac
    12061 tggacctaca ccattacgga tacgccttcg ggtgattcgg acctgattca gtttcaactg
    12121 ggcgatatga ccttggcggc tattagtgcg ggtccatact ttaagctcaa tgagtccatg
    12181 tccctcatgg tcaatgtagc cagcaaggat gaagtgacac gtttgtacca agccttgtca
    12241 gaaggcggtc gtattctcat gcctttggga gaatatcctt tcagccctta ctatgtctgg
    12301 ttggaagacc gctttggtct gtcttggcaa ctgtcttatg caccggactt ggacaagcct
    12361 tatcagtttg acatctgctt gctcttctcg caggaacagg ttggtttggc ccagcatatg
    12421 ctagattatt acaaggataa gctgcctcaa gctcgtcttg gtcgcttgtc ttactacggc
    12481 gaaggtgagg ctgcggtcgc tgctgccaaa ctgaattatg cagaacttct tgtcgctgga
    12541 cagaaaatga ttgttatgga ccacggctac ggtggcgtgg cttctttcaa cgaagccttc
    12601 tcattcatgg tctatgtaga cagtcaggaa gaagcggaga gctggtatga agaagtgtcg
    12661 gcagttccag aagcggagat ttgcggttgg gccaaggacc agttcgggat ttcttggcag
    12721 attgtgccgc gtattttgat ggaggcatac gatacggcta gtccagaaaa ggtcaaggca
    12781 gtcaatgcag cggtgatgac catgaaacgt ttggacattg cggctattca agcattgcta
    12841 aactagcaga tgataacgga gactgttctg cagtttccgt ttttttggtt gaggaaaggt
    12901 tgatttacag gaattttgat ataatggaaa aaagtgtacg aaaggaatgt gtatgaaagc
    12961 aacagaacga cgtaagaaaa tcctatccct tttacgagaa agcctcgatc cccttgcagc
    13021 taattatctg gcgaaagaat ttggagtgag tcggcaagta attgttggag atgtagcctt
    13081 gcttcgtgcg gagggagaag ctgtcttggc aactccaaga gggtacttac tccaatcttc
    13141 tttgccatct actgaaggag cctacattcg aaagatagct tgtcaacatg gtccagaaga
    13201 ggtagaaaaa gaattgacaa tcttgctaga gggtggagct caaatattag atgttgaaat
    13261 tgagcatccc atttacggct tattatcagg taagctgaat attgcaagtt tagaagatag
    13321 ggatcatttt ttggatgcct tgggtcaata ccaaggtgtg ttactgagta atttaacaga
    13381 aggagttcat acacatacag tctctttccc atctgtgcaa gcctataatg aactttcagc
    13441 agctttaaaa catgcaggta ttttactaga aaattaataa accttacagg tgtcttgaca
    13501 cctgtaaggt tttgttttat aatcaaacca atagaaagag aggtgcctaa tgtctcaatt
    13561 aacactatca tctaaaaatt cagttaagca gctatcaatt agtgcaattt taaccgcatt
    13621 tgccatttta atccctttaa tgatgccgat taaaattatt attggcccag catcctatac
    13681 acttgctagt cacgttcctt tgtttatggc catgtttgtt tcaccagcta ctgctatttt
    13741 tgtggcttta ggttctagtt taggcttttt cctcgccggt tttccaatcg ttattgtatt
    13801 tcgggcgctt acgcatctgt tcttccttac cttaggtgcg gtcttggtca agcgttttcc
    13861 gactctcatg gattctaagc gctctttact attaggaatt gggcttaatc tcctgcatgg
    13921 tctaggagaa tatattgtgg tgatggtgct tacttcgggg caacaaactt ctgctaccta
    13981 ctggataacg atgcttggct tggtgggggt tggctcggct attcatggat tgcttgattt
    14041 tagtttagct tattatttct ggaaaatttt aaaagaacgg aaaatttatc aaccataatc
    14101 agttatgatg aggaatatat tttagaagct ggagaatatt ctctagcttt ttttgggaat
    14161 ttttctggaa atgtacgata tttattcatt ttttaagaaa atatgcataa atatattgaa
    14221 aaaagtataa atatacacta taatagtcac taagttttat ttgctaatat atatagaaat
    14281 gagaaagttt atgacaaaag aaaaattaat tttggcctat tctggtggac ttgatacttc
    14341 agttgcgatt gcttggttga agaaagatta tgatgtgatt gcggtttgta tggatgttgg
    14401 agagggcaaa gatctcgact tcatacatga taaggcctta tcaatcggtg ctattgaatc
    14461 ccatgtattg gaagtaaaag aagaattcgc ccaagaatat gtcctaccag ccctacaagc
    14521 ccatgcctac tatgagcaaa aatatccgct ggtatcggct ctcagccgtc ccttgatttc
    14581 taaaaagttg gtggaaatgg ctcataagac aggagcaacc actatcgccc acggctgtac
    14641 tggtaaggga aatgaccagg tgcggtttga ggtagccatt gcagccctag atccaagttt
    14701 gaaagtcgtg gctcctgtcc gtgaatggaa gtgggcgcgt gaagaggaaa ttatcttcgc
    14761 caaggaaaat ggtgttccag ttcctgccga tctggacagt ccttactcag tcgaccaaaa
    14821 cctctggggt cgtgccaatg agtgtggtgt cttggaaaat ccatggaatg aagcgccaga
    14881 agatgctttt ggcatcacta cttctccaga aaatgcacca gatactcccg agtatgtcga
    14941 tattgagttt aaagcaggta ttcctgttgc tgtaaatggt gaaaagttaa gtcttgctaa
    15001 tctcattcaa agactcaatg tcattgctgg taaacatggt gtgggtcgga ttgaccacgt
    15061 ggaaaatcgt ttggttggaa ttaagtcacg tgaaatttat gagtgtccag gtgctattac
    15121 ccttctgaca gcccacaagg aaattgaaga tttgaccctg gtacgggaag tatcgcattt
    15181 caaaccgatt gtttcaaatg aactctctaa cttgatttac aatggtttgt ggttcaaccc
    15241 agcaacagat gccttgaaag cctatcttgc tcagacccag gcagttgtta atggcgccgc
    15301 aaaagtgaaa ttgtataagg gatcagccaa agtggttgct cggaaatctc caaattctct
    15361 ttatgacgaa gatttggcaa cctataccag tgcagatacc tttgaccaag atgctgcagt
    15421 tggttttatc aagctttggg gattaccaac caaggtcaat gcggaaattc ataaaaagag
    15481 ctagaaagta ggagcctatg gaagcaaaga aattatgggg cggtcgcttt gaagccagtc
    15541 ttgaggaatg ggtagaagaa ttcggagctt ccattcgttt cgaccaaaag ttggccaaat
    15601 acgatattca gggctcactg gcccatgtca agatgctggg gcaaactggg attatctctc
    15661 aagcggaggc tcaggtcatt caagctggtc tggaagaatt gctagaggag tatgaggctg
    15721 gcaagctggt ctttgatatt cgaaatgaag atattcacat gaatattgaa agtctattga
    15781 cggagaaaat aggctcagtt gcaggcaaac tccatacagc tcgttctcgt aacgaccaag
    15841 tggctacgga tatgcacctc tatctgaagg atacactctt tcaagttgta gacaaattga
    15901 ccaacctacg tcagatttta gtagaccttg cacaggagca tgtggaaaca atcatgcccg
    15961 gctataccca cttgcaacat gcccagccca ttagttttgc tcagcacttg ttagcctatt
    16021 acaacatgtt cagccgagac agtgagcgct ttgcattcaa catgcagcat acaaatgtat
    16081 ccccacttgg tgcggcggcc ttggcaggga cgacctttcc gattgaccgt cagatgacgt
    16141 ctgatttgat gggatttgct cagccctata gtaattctct tgatgctgtg tctgaccgtg
    16201 attttatctt ggaatttctg tcaaatgcca gtatcctcat gatgcacatg tcgcgtttgt
    16261 gtgaagaaat cattaactgg tgttcacatg aataccaatt tgtcacccta tccgatacct
    16321 tttcaacagg ttcttccatc atgcctcaga agaaaaatcc agatatggct gagctgattc
    16381 gggggaaaac aggtagagtt tatgggaact tagttggcct actgacagtc atgaaatccc
    16441 tgcctttaac ctataataag gacttacagg aagacaagga aggcatgttt gacacggcag
    16501 aaacagtttt gatttcgatt gatattttgg cagggatgtt gcaatctatg acagtccata
    16561 aggaaaggat ggctcaattg acagagaagg acttttcaaa tgctacagag ttggctgatt
    16621 atttggctag taaaggtctg cccttccggc aagcgcatga gattgttgga aaactggtat
    16681 tggagtgtag caaggcagga cactgtctcc aggatgtatc atttgagacc tatcaggcta
    16741 tttcgcctct tattcaagca gatatttatg atgccttgtc ctccaaagtt gctgtcagtc
    16801 gtagaaattc cctcggtggg acaggttttg aatcgattgc agaccagttg aaaagtgcca
    16861 aagaggaaat tcagaacgca aaaagcctat aaacggtatg tttattggct tttttgaatt
    16921 ttgtggtata atagaagtaa ttttgaaaga tggagtggca gtttgaaaaa aagctatcgt
    16981 gtcaagcgtg aacgagattt taatgacatt tttacaaaag gaaacaatgt cgccaatcgg
    17041 aggtttgttg tctaccgatt acctaaagag caaaagcatt tccgagtagg tttatctgtc
    17101 agcaaaaaat tgggaaacgc tgtcatgcga aataagatta agcgcaggat tcgtcacatc
    17161 ttaattgaat gccaacatga actggtagca gatgactttg ttatcattgc tcgaaaaggt
    17221 gtagaggaat tgtcctatca agaaatgaag caaaacctca tccatgtttt gaaactagct
    17281 aaactatacc aggaaggtgt tgtgattgaa aaagaaagtt aaatgggcag gtctactggt
    17341 agcatctcta attgttttaa cagcctgcgg aacagggcca gtgaccagtc aatctacaga
    17401 tgcgtgggat agattaattt actggtttgc cagcattatt cagttcctat ctatcaacgg
    17461 tcaaattgga atcggtatta tactttttac cattttaatt cgaactcttc tgttgccttt
    17521 gttccaaata cagatgaatt cctcacggaa gatgcaggag cttcaacctc agctgaaaaa
    17581 gttgcaagcg gagtatccag gaacggatat ggaaagtcgc cagcagcttt atgaagcaac
    17641 gcaagctctc tataaagaga atggcgtcag catgagagcg tccatgattc cgctctttat
    17701 tcaaatgcca atccttttgg ccctctttca agctctgaca cgtgttgaag ccttgaaggt
    17761 aggtcacttc ctttggttaa atcttgggga gccggatccg ttctttattt tgccgattct
    17821 agctgcagtg tttacattcc tcagctcatg gttgaccaat aaatcggcaa ttgagaagaa
    17881 tatggctatg acaattatga cctatgtcat gcccgtgatg atctttttct ttgctgtggc
    17941 agctgctagt ggggtggctt tgtattggac agtgtcaaat gcctatcagg ttgctcaaac
    18001 cttggtgttc aataatccgt ttaaaataat agcagagcgt caagcgaaaa ttgatgcgga
    18061 aaaagagcgt cgggcaaaaa tccgacgggc acagaaaaaa gcacagaaaa agaaatagga
    18121 aaactaggag agagaagatg aaatttacag gagcaagtgt tgaagaggcc atccagaatg
    18181 gcttggtaga gctgaatatt cctcgaaaaa aagcacatat tactgtggtt gcgcgtgaga
    18241 aaaaaggttt ccttggtttc ggtaaaaagc cagcgattgt ggatattgat gtcatcaatg
    18301 aaacaacggt tgttaaggct aatcagaaag cagttcgtgg cgttccaagt gaaattaatg
    18361 acctcaatga accagtaaaa agcgtttcag aggctacgat tgacctagga aaagttgttg
    18421 cagcggtgaa agaatttgaa aagtctggtc aatcgcttga tgatgatatt aaagcgcaga
    18481 tcttgaaaaa tgaaaaagaa gcgacaacga ttttggaaga aacgggtcgg attgaaattc
    18541 tttcagagga tttactaact actacagatg caacaacatc tgctaaggaa gatgtaaatc
    18601 aagaagtcat ctcagaaaat caagcagata attttgctga tttagagatt aagattgagc
    18661 cccagtatga tattgaacaa gttgtttcag aagtgacagc ttatgtacaa gaaattctgg
    18721 atgatatgga tgttgaggcg cgtattgata caagtcataa tcgccgtaca atcaatattc
    18781 aagtggatac caatgaacca ggtcgtgtta tcggttatca cggaaaagtc ttaaaggcct
    18841 tgcaactttt ggctcagaac ttcttataca accgttatga gcgtaatttc tatattacaa
    18901 tcaatgtgaa tgattatgta gaacaccgcg cagaggttct tcaaggctac gcacaaaaat
    18961 tggcagaacg tgttttggca gagcaggaag cctaccatac agatccgatg tcaagtagcg
    19021 aacgtaaaat tatccaccgc attatctcta aaatggatgg tttgacgagt tattcagaag
    19081 gtagcgagcc gaaccgatat gtggttgtag atattgaagg gaatcatgat taaaaagagg
    19141 ctagtcctct tttttcaatg attttctgat tctttcctaa aagtatggca gaatagcttg
    19201 acaaactaac agaatttata taaaatagtt tggtatgttt agtaactgat caaaactagc
    19261 cggctaaacg aatacaagaa tctaagagga ggtattcatc gtgaaacgta cttttcaacc
    19321 aagtaaaatc cgtcgcgcgc gtaaacacgg tttccgtagc cgtatggcaa ctaaaaacgg
    19381 ccgtcgcgtg ttagctgctc gtcgccgtaa gggacgtaaa gttcttacag tagcataaga
    19441 agatgaaaag agaagccagc agaaactcga gtctgctggt ttttttcttc tcataagaat
    19501 catctgaaat atgttacaat agtaggaaag aaagtgatgg aacgatgata caaatttttg
    19561 acacccacac ccacctcaat gtagagcagt ttgacggtcg ggtccaagaa gaattagact
    19621 ttgccaagga aatgggggtc accctccaca atgtggtcgg ttttgaccgt gtaaccattg
    19681 acaaggctat ggagcttgct gaccagcacc ctgaaattta tctgacactg ggctggcatc
    19741 ccacagaggc tggcacttat gacgaggaag tggaaaacta tctgctcaag gctctccagc
    19801 accccaaggt cattgccctg ggagagattg gactggatta ctactggatg acggcggata
    19861 aggaaaccca ggcccgtgtc ttcaagcgac agatggagtt ggctaagcag ttggatttac
    19921 cctttgtggt tcacactcgt gatgctttgg aagatactta ccagatcatc aaggaagtcg
    19981 gcgtcggtcc acgcggaggg atcatgcatt ccttctctgg cactctggag gaggctcagg
    20041 ccttcatgga cctgggtatg cacatctcct tctcgggagt agtgactttc aaaaaagctg
    20101 tcgaacttca ggaagcggcg caagcattgc ccctagaaaa aatcctggtc gaaacagatg
    20161 cgccctacct ggccccagtt cccaagcgtg gtcgggaaaa tcgtaccggc tacaccagat
    20221 atgtggtgga tatgctggcc cagcttcgca accagtcggt cgaagaggtg gcgagagcga
    20281 cctgcgagaa tgcaaggagt ttgttcagaa ttgactcaga aaattaagat acaagaagtc
    20341 ctcgttgtcg agggaaagga cgatacggca aacttgaagc ggttttatga ggtggatacc
    20401 tacgaaacca gaggctctgc catatcagac gatgacttag aacgcataga gaaactccat
    20461 gatctgcggg gcgtcattgt ctttactgac ccagactaca atggcgagcg gattaggaaa
    20521 atcattatgc aggccattcc cacggtcaaa cacgccttct tacagcggga cgaggctgcc
    20581 cccaagtcca aaacaaaagg gcggtccttg ggcattgagc acgccagctt tgaggacttg
    20641 gaaaaggccc tcgcaggcgt gcgtggctac tatgatgatg aacataactt tgacatcacc
    20701 aagtcagatc taatgcgcct agggctcctt atgggtggcg acagtcgcaa gcggagggag
    20761 tatctgggag aaaagctccg tatcggctac tgtaatggta agcaactcct caaacgcttg
    20821 gagctgtttg ggattagtct ggcggaagtt gaggaggcaa tggggagcta tcatggatag
    20881 ggtctatgct gttaagtgct tggttaaaaa cgagtttgaa ccttcttatg aaaattattt
    20941 atcttatgaa gaagtgacct acttgctgcc ggctgatgtc cttgtttgga aggatgagca
    21001 ggttgataag aacaaactag cagactattt gtgtggttta gttgaagatg gctatgtcaa
    21061 tgcggatggt atttggaagc agagccgttt gtttcatatt gccggtgtgc aagaaatgga
    21121 cgacagggta gagtttgatg gaccagccgt ggaagtctat cgctggtttt acaattttga
    21181 acgtccagtt actgaggaag aatttttgaa tctctattat tttgatgatt tggggaggta
    21241 aacaatctag agtggaaatg ttactatttc tgcttttttg aagattgtta ctgatatcta
    21301 ttcgtgttac aatatagata actaggaggt agactagatt gataactata aagaaggatg
    21361 cccaagtaaa ttttaaaact gattccagac ttttggaaaa agccaaggaa gtatttgcta
    21421 tgaaccagct ggatttgact gctggcttta acctcttctt gcaaaatatc gctgtaaaaa
    21481 atgagttgcc aattctgaca gaagaagagt tggaaagaga agagttgttt ttacagttac
    21541 agaaggaaat ccaggaaaac caacaggcga ttgaggcagg tcacgggatc agcctagaaa
    21601 gtgtcaaaga gaagtatgga atttgagtct tattcagtga tacttgctcc agcagtggag
    21661 aaggaattgg cagttattta cgcctatttt tcggaacagt tctcagaaga aattgccaaa
    21721 agacgaattg ggatgattgt cgaagcattg gagagtttgc aaatttttcc tgaacgtggt
    21781 tttaacgccg ataatcgttt tggtaagcag atagacccgc ctcatctaac ccgaggctat
    21841 gtcgttggaa aagactacat cgccctctat cgcgtagtga aaaatgaagt tcgagttggt
    21901 cacctttttg cgaccaagag tgattatgtg aagttgttga agtgagagag catgagtagg
    21961 acagagcaag tcatcctaac caatatgtgc atgattacag acggtcagca ggtattagtt
    22021 caggatagga agtcaaagaa atggtctggt gtgactttcc ctgggggcca tgtcgagcca
    22081 aatgagtcaa ttatttcctc cgtcattcgt gaaatcaagg aagaaacggg tttaacggta
    22141 tccaatctcg aactctgtgg tattcaaaac tggacggatc caactgacca ttatcgctat
    22201 ctggtatttt gctacaaaac cagtcagttt tcaggtgttc ctcaatcctc agatgaggga
    22261 gaggtcttct ggattgaccg cgcagaattg aagaatgttc agctggcaga cggttttgaa
    22321 accatgttgg aaatctttga acagccacag ctgatggaga atttttactg gtttgaagac
    22381 ggtgagtgga agatggagaa tcgctaaaga gttgtacaat aagtttgaca actctttttt
    22441 tctatgctat agtaagccta gaaatgatga gaggtattgc atatggaagc tattgtatat
    22501 tcccattttc gaaatcactt aaaggactat atgaaaaagg tcaatgacga gtttgagcct
    22561 ttggtcgtgg ttaataaaaa tccagaggaa gatattgttg tactctcaaa gagcgagtgg
    22621 gatagtttac aagaaaccct cgctgttgct cggaacactt atttgtccca aaaagtccta
    22681 cgtggtatgg ctcaagtcaa agcgggacaa actcaagaac gcaatctgat agaggcggac
    22741 taacatggga atccatttca cagaggatgc ctgggaagac tatctttatt ggcaaggtca
    22801 ggataagaaa acactgaaga gaatcaataa gattatcaag gatatgcagc gacatccttt
    22861 tgaaggcatt gccaaaccag aacctttgaa gtatgattat caaggtgcct ggtcaagaag
    22921 gattgatgcg gagaatcggt tgatttatat gatggaagct gatcggttgt gcatcctgtc
    22981 gctaaaggat cattataggt gagggggatt aacaaatgaa aaaagtcgtc ctccttcgag
    23041 gtgttacccc tgttggcaag aataaaattc ctaagatgtc ttacttggcg gaaattttga
    23101 cggaagttgg tttaacttct gtccaaacct atattcaaag tggaaatgtt gtttgtgaaa
    23161 cagatatatt agatgaagaa ctcagtcggc ttatccatca aaccattaaa gaaaaaatag
    23221 gtgcagagtt agcgatcatt gtcaaacatc agatggagtt aacacaagca gttgctgaaa
    23281 atccttttgg tgagggttat gatccttctc gtgtccattt ggtctttacc aatgatgaga
    23341 tagctggtga aaaattaaac aaattattgc accaagactt tggtgatgag gagttgcgtc
    23401 tgggaagtca atgtctatac atgtatcttc cgagagatgc tcgaaagaaa aaactaaata
    23461 ccaattattt agaaaaacag ctggacatta cagcgacgat gagaaaacta agtgttattt
    23521 cacgattgag tgaaatggcg aaataaaaaa caagaaagaa cacacatgag aatcgcagac
    23581 aaaaacgtta ctcgtgccgt cctggaacgc cacgggttta cctttaagaa atccttcggt
    23641 cagaactttt tgaccgatac taatatttta cagaagattg tggacacggc tgagattgac
    23701 cagaatgtca atgtcatcga aatcggtcct ggaatcggag ccttgacaga atttttggcg
    23761 gagagtgccg ctgaggtcat ggcctttgag attgacgacc gcctgattcc gattctggcg
    23821 gataccttgg gccactttga caatgtgacc gtggtcaatc aggacatctt gaagaccgat
    23881 ttgcagaccc agattcagaa tttcaagaat cctgacctgc ccatcaaggt cgtggccaat
    23941 ctgccttact acattaccac tcctatcctc atgcatctga ttgagagcaa gattcctttc
    24001 agcgagttcg tggtcatgat gcagcgggag gtggccgacc gtatttctgc cgagcccaat
    24061 accaaggcct atggcagtct gtccatcgcg gtccagtact acatgactgc caaggtggcc
    24121 tttattgtgc cccgtaccgt ctttgtgccc gcgccaaatg tggactccgc cattctcaaa
    24181 atggttcggc gcgaacaacc cttggtcgca gtccgtgacg aagatttctt ctttcgggtg
    24241 tccaaggcca gctttgttca ccgccgcaag accctgtgga acaacctgac caaccatttc
    24301 ggtaagtccg acgagatcaa ggataagctg acgcaggcac ttggcatggc agagctggaa
    24361 gccagtgttc gtggagaagc actttccatg gctgacttcg cgcgtttgtc tgacaggctc
    24421 caagaagtcg gtttataagc aagtattgat tgcggagaaa tctgcaatca atatttattt
    24481 tatgataaat cactttcccg ccttcacgcc aaacgcatga a
//
LOCUS       DATUCG010000018        23388 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.018, whole genome shotgun sequence.
ACCESSION   DATUCG010000018 DATUCG010000000
VERSION     DATUCG010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23388
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.018"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            492..1307
                     /locus_tag="VCA20_000598"
     CDS             492..1307
                     /locus_tag="VCA20_000598"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEP1834453.1"
                     /translation="MNQQFSLDQFSLKGKIALVTGASYGIGFAIASAYAKAGATIVFN
                     DINQELVDRGMAAYRAAGINAHGYVCDVTDEAGIQAMVAQIESEVGIIDILVNNAGII
                     RRVPMIEMTAAQFRQVIDIDLNAPFIVSKAVIPSMIKKGHGKIINICSMMSELGRETV
                     SAYAAAKGGLKMLTKNIASEYGEANIQCNGIGPGYIATPQTAPLRELQKDGSRHPFDQ
                     FIIAKTPAARWGEAEDLMGPAVFLASDASNFVNGHILYVDGGILAYIGKQPEA"
     gene            1359..2000
                     /locus_tag="VCA20_000599"
     CDS             1359..2000
                     /locus_tag="VCA20_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEP1834454.1"
                     /translation="MKIALINENSQAAKNSLIFETLKSVTDAKGYHVFNYGMYGKEGE
                     SQLTYVQNGLLASILLVSKAADFVITGCGTGVGAMLAANSFPGVVCGFAVDPTDAYLF
                     SQINGGNAVSLPFAKGFGWGAELNLKLLFERLFAEEMGGGYPRERVIPEQRNARILNE
                     MKAVTYKDLLTVLQEINQDFLKETISGEHFQEYFFANCQDESIAAYLKTVLES"
     gene            2017..3018
                     /locus_tag="VCA20_000600"
     CDS             2017..3018
                     /locus_tag="VCA20_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEP1834455.1"
                     /translation="MKKVLLIGEPLIRITPTNYQELTDGVESRVFFGGSEINVACNLQ
                     GFGCETKLLTALPQGSLGDRFLDFLDEKGIDTSSVQRKGERVGVYYMEAGFGCRSSQV
                     YYDRGQSSLEAIQVDHFDMDQLFLEVGLVHFSGITVAIGSAVRAWLVQVLKEAKRRKI
                     PISMDLNWRSKMIGLRDAKKVFSQFATYADYCFGLEPIMANEKDGELFERDTTTVHTI
                     RERMQTLKEAYKFQAIFHTIRQTDELGRNLYKAYGLADEFCCSQELKTQVLQRVGSGD
                     AFVAGALHKLLEGTSLKAILDFAVASGTYKCSIEGDHMRQSLRKVESLLEGKNDIIR"
     gene            3029..3670
                     /locus_tag="VCA20_000601"
     CDS             3029..3670
                     /locus_tag="VCA20_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEP1834456.1"
                     /translation="MSPSQIISKLKEQGLVIVVRGDDKEDGFKTAQACIAGGITAIEV
                     AYTNPDANMIITELSRCHKDDVSVLIGAGTVLDSVTARQAIMAGASYIVSPSFNIEVA
                     KICHLYAVPYLPGCMTLTEITNALEAGCEMVKLFPGSVLGTNYITAIKGPLPHVSIMV
                     TGGVRLNNVSEWFNAGATVLGIGGEFNQLATKGEFGTIQELASSYTSVRDKMK"
     gene            3957..4391
                     /locus_tag="VCA20_000602"
     CDS             3957..4391
                     /locus_tag="VCA20_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEP1834457.1"
                     /translation="MIKVIVVAHGQFASGILTSLKLIAGEVENIEAIDFSEGMSAQEL
                     KARIKSAILGEREVLILTDLLGGTPFKVSVELAAEQKEQNVVVLSGLNLAMILEANFS
                     RLTDDLEQLVGKLITTSKDGIVDSVSLLAEDNHDEELFEDGI"
     gene            4403..5593
                     /locus_tag="VCA20_000603"
     CDS             4403..5593
                     /locus_tag="VCA20_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEP1834458.1"
                     /translation="MIKDVLIESIAKKREFRNFTLLSKKEVEAAIELAIQQLAINCEY
                     FGESFPTPATFDNHYPKMDNTEWTNGFWTGALWLGHEYSLNNEMKALAKKNTSSFLER
                     VKQRIELDHHDLGFLYSPSTVADYKLTGSKEALEASLLAADKLMERYQEKGGFIQAWG
                     QLGKAEDYRLIIDCLLNLQLLFFAYEETGEQKYYDVALSHFYASVNTVIRDDASTFHT
                     YYFDPETGKPLKGVTRQGFSDDSSWARGQSWGIYGIPLAYRKIKDPACLNLFKGVTNY
                     FLNRLPEDNVSYWDLIFNDGSGHARDSSATAIAVCGIHEMLKYLPEVDEDKETYKYAM
                     HTMLRSLIDNYANRELAPGRPLLLHGVYSWHSGKGVNEGNTWGDYYYLEALLRFYKDW
                     ELYW"
     gene            5603..6094
                     /locus_tag="VCA20_000604"
     CDS             5603..6094
                     /locus_tag="VCA20_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEP1834459.1"
                     /translation="MATPNIVMARIDERLIHGQGQLWVKSLGCNTVIVANDKVSEDKI
                     QQTLMKTVIPDSVAMRFFSLQKVIDVIHKANPAQTIFLVIKDLQDALTLVKGGVPITE
                     LNIGNIHNAPGKTQVTRSIFFGSEDKAIVRELNDVYKIRFNTKTTPSGNDGAVEVNIL
                     DYI"
     gene            6113..6889
                     /locus_tag="VCA20_000605"
     CDS             6113..6889
                     /locus_tag="VCA20_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEP1834460.1"
                     /translation="MTISLIQAALIGLWTAFCFSGLLLGLYTNRCIVLSFGVGIILGD
                     LPTALAMGAISELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVTPEAALALST
                     PIAVGIQFLQTSAYTLHAGAPESAIKHLRAGNLSKFKRVANGTVITFAFLGFALGFLG
                     AYSMDTLLKLVEMIPPVLLTGLSVAGKMLPAIGFAMILSVMAKKELIPFVLLGYVCAA
                     YLQLPVIGIAIVGSIFALKEFFNKPEQVAVKQEEAHDDWI"
     gene            6876..7691
                     /locus_tag="VCA20_000606"
     CDS             6876..7691
                     /locus_tag="VCA20_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEP1834461.1"
                     /translation="MTGSKLTKKDYLNTSLRAFFLQNGFNYSNYQGIGYANVMYPAFK
                     KHFGDDKEGLTKALEENCEFYNTNPHFLPFITSLHLVMLENDLPEEETRGIKMALMGP
                     LAGIGDSLSQFVLAPLLSTIAASLAGQGLVMGPILFFLGMNIILTSIKLLTGLYGYKF
                     GTSVIDKLSDQMATISKVANIIGVTVISGLAVTFVKISVPITFAAGELTEGADQKIVT
                     IQGMLDKIAPALLPALFTILVYYLIKNKKWTTYKLVILTVIIGILGSWLGILA"
     gene            7698..8006
                     /locus_tag="VCA20_000607"
     CDS             7698..8006
                     /locus_tag="VCA20_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEP1834462.1"
                     /translation="MDQQTLYYALIILVALSTILPVLTSYVNRKRQQKAVEEQLEKRQ
                     RYLSTLQAGDEVILFSGLHGKIFKLTDSLVELQIAKGIIVYVEKESIMGKAKELLFAK
                     "
     gene            8099..11602
                     /locus_tag="VCA20_000608"
     CDS             8099..11602
                     /locus_tag="VCA20_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEP1834463.1"
                     /translation="MGIFTSQTKRYYSIRKYKIGVCSALIALSILGTRVAANQLPSTE
                     TASPQTSQLVETTPETAETATLTTTSEVSSEASPITSTESQPSSTTAETLASPQAVQA
                     TKEEKNLVANGEFISTTAPSGNWKESAATDWETWIPNEIKKENGAVSIDDGKLHISST
                     NTYRVAVHQTVDVDPSKRYLFSYDVETKDLKGSGVRVRLRSLTAEGKDLSPQEFTFTN
                     FVAGTQQRKVEKELTVSPETRKLKVELFFENGIGQAWLDNISLVEYVEKTPETPEPSP
                     ELVQPETGQISLASNKVYLPVRPDLTYQIADAAVATVEKIWIRPLAAGKTQVDVYDKD
                     TKLSSFELTVTEHQATVFDTLRNNWEDISLANKRYQSNDTQMKAFLGRLDAGVASSLK
                     KWVEPTDQGKTIFNDIDFSKSSHLTTVYRRLEQMAQVVENPDSAYYHDRSLIDLVRKG
                     MNWLYTNVYNENKSIDGNWWDYEIGTPRAVVNTLIYMHPYFSQEEILTYTKPISKFVP
                     DPTTIRKTLTNPVPAVGGNQTDLSKVAILEGALREDADRVRAGAQGLTTIMKFVDKGE
                     GFYRDGSFIDHTNVAYTGAYGNVLIEGFSQLLPVIQPTEFALKEEQTNILYEWIEKAF
                     MPILVRGELMDMTRGRSISRATGESHVQAMEILRSLVRIAESAQPEQKTKLLSFVKAQ
                     LTSDTFYDSYRSLKSYKDIDLVNKLLADNQIPVEVDKDYIAAFNNMDKFVYRSAQEGF
                     TFALSMYSSRTQNYEDMNNENRKGWYTADGMVYLYNDDLSHYSNHYWATVDPYRLPGT
                     TTTKDKREDGSGEVTLASDFVGASQLGNRLATIAMDFNNWNNSLTARKAWIVLGNKIV
                     FLGTDIQHQSAQGAETTIENRKLLTGEKYSYYINGQPVDLSKEVVTDKTQSFYMTNGR
                     DNQSIGYVFLNQLPTHAKLDQRTGKWSDINYNQSKEEVSNSFVSLWHEHAQTSSNYAY
                     VLVPNQSMEKVNQAAASVKLLHQDRDLQVVYDQEQNVWGVVKYTDTAYKLTDDITLTD
                     AGLYTIQKVEGGYRIAFYNPSTRTVKNGIELTKAGSSLTVEMEPTAACPSTVWKVTMP
                     EGSDKQTGSIEKTEKEEKQLKENQPSPEVKQVVHHAAEKTKPSKPRLPQTGEEASLGL
                     GFLGLLTLGAVVDFKCRRSRS"
     gene            11861..13837
                     /locus_tag="VCA20_000609"
     CDS             11861..13837
                     /locus_tag="VCA20_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEP1834464.1"
                     /translation="MNFEEWIWALPSLSSFGEAIEKGLVMQTIFGKIVANQSAESVKT
                     YILNQQDAAYKELLLSCQNLLNDIFIFEDNWDMEPCSVGYHLAPLDWTADCHRDEEWT
                     FMLNRQEYLWKLVLAYLVEKDSRYMEQVKRFIFSWIEQVTDWHPKRTSSRTLDTAIRC
                     LSWLNILPFLIDWGGLSEAERSQLLVSIQKQLSYLHSHYRAKDTLSNWGIFQTGAMLL
                     AHAYIGDQVDLVELHHFARQEIQEQIQTQILEDGTQFEQSFLYHVEVYKLLLGLAFGL
                     PDYRKQWTPILKKMADYVLQMTGPDGKSVALGDSDVHCTSDILQLTAIFFEDGTYLVD
                     NSKLDLGVLFLVGENGLRIFEHLTAQPSSLGGQHFSESGHSIVKEVDSYLCFKAGPMG
                     SAHSHSDQNSLCFYHKGQPIVIDSGRFSYRECEERYLLKSAWAHSTCILDDNAPEQIK
                     GSWEYDTYPQFISHSYKGFGRCHWMQGIYQAQTSQGLPYWHKRQLLMVGGDLLLIVDS
                     VDCSGQHELTSQLILDERVKIGEEQLNDLRLYSQKDFEIQKTVRSPRYNQLVETNKLV
                     KKESFRDRLVTFTLLADEAIRVKQLPVYQTDYHLIESGLAFSCQGKGLNILLVVSDND
                     IYKGEKLLQVAGYKLRGKCLVVDRDKRQVVRLKN"
     gene            13934..14935
                     /locus_tag="VCA20_000610"
     CDS             13934..14935
                     /locus_tag="VCA20_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEP1834465.1"
                     /translation="MSEKFTIKDIAELAQTSKTTVSFYLNGKYEKMSKETRERIERVI
                     RETDYKPSVVARSLNSKKTKLIGVLIGDITNTFSNQIVKGIEEEAHKSGYQVIIGNSN
                     YNQESEDKYIESMLMLGVDGFIIQPTSHFRKYSRIIEDKKKHMVFFDSQLYEHRTSWI
                     KTNNYDAVYDATQLCVENGYQKFILITADISRLSTRIERATGFMDALLDCKQSYQDLT
                     IADENTDIEEIKRFLQGVVNPQEKTLVFVPNCWALPLVFNAMKELKYDFPQVGLLGFD
                     NTEWTNFSSPTISTIVQPAFEEGCQATRMLINQIEGGNQVGRQQVLDCTVHWKESTF"
     gene            complement(15356..16315)
                     /gene="nrdF"
                     /locus_tag="VCA20_000611"
     CDS             complement(15356..16315)
                     /gene="nrdF"
                     /locus_tag="VCA20_000611"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEP1834466.1"
                     /translation="METYYKAINWNAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLTAEEKDLVGKVFGGLTLLDTLQSETGVQALRNDIRTPHEEAVYNNIQFMESVHAK
                     SYSSIFSTLNTKSEIEDIFEWTNSNEYLQRKAKIINEIYETGTPLEKKVASVFLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELSEEEQDKLR
                     DWMYDLLYQLYENEEGYTRSLYDAVGWTEEVLTFLRYNANKALMNLGQDPLFPDSADD
                     VNPIIMNGISTGTSNHDFFSQVGNGYLLGEVEAMTDDDYLYGL"
     gene            complement(17146..19305)
                     /gene="nrdE"
                     /locus_tag="VCA20_000612"
     CDS             complement(17146..19305)
                     /gene="nrdE"
                     /locus_tag="VCA20_000612"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEP1834467.1"
                     /translation="MSLKELGDVSYFRLNNEINRPVNGQIPLHKDQEAVKAFFKENVL
                     PNTKQFDSILDKIAYLLEENYLEKEFLDQYSPEFIIKIDQFLKDQNFRFKSFMAAYKF
                     YNQYALKTNDGAYYLESMEDRVLFNALYFAEGDEELALNLANEMIHLRYQPATPSFLN
                     AGRDRRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGISLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLDVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGITVPDKFYELARKNEDMYLFSPYSVELEYGVPYSYLDITEKYDELVANPRI
                     RKTKIKARDLETEISKLQQESGYPYVINIDTANRSNPVDGKIIMSNLCSEILQVQTPS
                     VINDSQEYLTMGTDVSCNLGSTNIVNLMKSPDFGLSVRTMTRALTYVTDHSHISAVPS
                     IEAGNERAHSIGLGAMGLHSYLAQNLIDYGSKTAVEFTNIYFMLLNYWTLAESNNIAR
                     ERKATFHNFDKSKYADGTYFDKYVTGDYQPQSDRVKELFEGIFIPSGQDWAELREKVM
                     ADGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLATETI
                     PFYKSAYDMDMRKVIDVYAAATEHVDQGLSLTLFLRSELPKELYEWKKENKQTTRDLS
                     ILRNYAFNKGVKSIYYVRTFTDDGEEVGANQCESCVI"
     gene            complement(19338..19556)
                     /gene="nrdH"
                     /locus_tag="VCA20_000613"
     CDS             complement(19338..19556)
                     /gene="nrdH"
                     /locus_tag="VCA20_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HEP1834468.1"
                     /translation="MVTIYSKNDCVQCKMTKKFLDQHSVDYKEINLDEQPEFIDHVKG
                     LGFSAAPVIETENDVFSGFQPGKLKALV"
     gene            19942..22611
                     /gene="acnA"
                     /locus_tag="VCA20_000614"
     CDS             19942..22611
                     /gene="acnA"
                     /locus_tag="VCA20_000614"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEP1834469.1"
                     /translation="MGKSHRKRLFHKGKEYSYYALDSISMEEKVDIHSLPYTIRILLE
                     SLLRKEDGVDVTKNHIMELLHYQAASPKGEIPFKPSRVILQDFTGVPVVVDLASMRDA
                     VVKAGGNPELINPEIPVDLVIDHSVQVDFFGTEDALEKNIALEFERNNERYEFLKWAE
                     NSFENYRAVPPATGIIHQVNIEFLSDVIINNDGLLYPDSMFGTDSHTTMINGIGVLGW
                     GVGGIEAEAAMLGEASYFPVPEVIGVRLAGQLPKVATATDLALKVTQLLRQENVVGKF
                     VEFFGPGLSSLTLADRATVSNMAPEYGATCGYFPIDGETLHYMRLTNRSEEHVELTEV
                     YAKANYLFYDAERFPSYSKVLELDLSTVVPSISGPKRPQDLIELTDAKAEFQASLIRE
                     VGVRGFGLEEAELDKTATVKYVEGDEQIQTGHVAIAAITSCTNTSNPYVLLAAGLLAK
                     NAVEKGLAVSKTVKTSLAPGSKVVTGYLKKSGLQTYLDALGFNLVGYGCTTCIGNSGD
                     LRPEVAEAIKEEDLLVSAVLSGNRNFEGRINPLVKANFLASPPLVVAYAIAGNMNVDL
                     TRDPLGYDEKQQAVYLADIMPSREEVDDYIERYVTRDLYKEEYQQVFIDSQAWNAIET
                     KTDKNYNWNSSSTYIQNPPYFDNMQADLSIKPLENLSVLAKFGDSVTTDHISPAGNIA
                     RLSPAARYLEENGIVYKDFNSYGSRRGNHEVMMRGTFANIRIKNELADGKIGGWTRVA
                     DELLPIYDAAMKYKEAGVGSIVIAGKDYGMGSSRDWAAKGSSLLGVKAVLAESFERIH
                     RSNLVMMGVLPLQFLEGQSAESLGLTGHESYTIDLPEDVGVGQIVTVHAQTDDVTKEF
                     QALVRFDAEADIRYYRYGGILPMVVRKKLGGKV"
     gene            22608..>23388
                     /locus_tag="VCA20_000615"
     CDS             22608..>23388
                     /locus_tag="VCA20_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019805873.1"
                     /note="catalyzes the formation of citrate from acetyl-CoA
                     and oxaloacetate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEP1834470.1"
                     /translation="MTETNGLKDAIACDTRISAIENDRLSYAGYDIAELMENNASFEE
                     VIYLLWNLHLPTQIELNYFEKSLRKEYAISDAVEQCILIQSRRHLHPMSVLRSTVSLL
                     GVYNVHAEENSLEALYEQSIQIMAKIPTIIATFAQLRRGLVPIEPRSDLGFAANFLYM
                     LNGEEPSDLQIKAFNKALVLHADHELNASTFAARVTASTLTDLYSCVTTAIGTLKGSL
                     HGGANERVFDMLLAIRESGDTEKYLQKKLDSKEKIMGFGHRV"
BASE COUNT         6985 a         4075 c         5394 g         6934 t
ORIGIN      
        1 atgattgaga tagatggtac cttgttgcac gttgcaatag ggtatttttt atttagagta
       61 gtaaacacag ggtaaaaatg taaatgtttg agttcctatt aacatctcgg cggagtggtt
      121 gagttttcga atattctgga gattgtttga ggtgggaaat tggtagtgag ggagtcagtc
      181 gtatcacttc ccgctcccat tttgtatggt ttactttcgt gaatgtagta tagccaattt
      241 tggataatat atgaataatg acatgtaaaa tgtttactaa tgattgataa aatcgaagaa
      301 gaaaatcata tttgtgttaa gtatatttta ttgcttgggg aatgaagata ctcgaatttt
      361 ataagattat atcaagattc gttttgaaag tatttaaaat aaatgataaa aaccgttgac
      421 aaaaccggtt tactatatta caatacaatt tgtaaacgct ttacaaatta aaatacaagg
      481 gagttacgaa tatgaatcag caattttcat tggatcaatt ttctctaaag ggaaaaattg
      541 ctttggtaac aggggcgtcg tatgggattg gctttgcgat tgcatcggcc tatgcaaaag
      601 caggggcgac aattgttttt aatgatatta atcaagagtt ggttgatagg ggaatggcag
      661 cctatagggc agctggtatt aatgcgcatg gctatgtttg cgatgtaact gatgaagctg
      721 gaatacaggc tatggttgct caaattgaat cagaagttgg aattattgat attctagtga
      781 ataatgctgg tattattcgt agagttccaa tgattgaaat gacagcggct caatttcgcc
      841 aagttattga tattgaccta aatgctccgt ttattgtttc taaagcggtt attccatcta
      901 tgattaaaaa ggggcatggg aagattatca atatttgttc catgatgagt gagttaggac
      961 gagaaacagt gtctgcttat gcagcggcta agggtgggct taagatgttg acgaagaata
     1021 ttgcttcgga atacggtgaa gccaatatac aatgtaacgg cattggtcct gggtatattg
     1081 caactccaca aacagctccg ttacgtgaac tccagaagga tggttctcgt catccatttg
     1141 atcaatttat tatcgcgaag acccctgcag ctcgttgggg tgaagctgag gatttgatgg
     1201 gtccagcagt ctttttagca agtgatgcta gtaattttgt gaatgggcac attctttatg
     1261 ttgatggagg aattctagct tatattggta aacagccgga agcgtaataa acgaattaac
     1321 aaatttaaca aattttgata taagaagggg aaaaaacgat gaaaattgca cttattaatg
     1381 aaaatagtca ggctgctaaa aatagtctta tttttgaaac attgaagtca gtaacagatg
     1441 ctaaagggta tcatgttttt aactatggaa tgtatggtaa agagggagaa agtcagttga
     1501 cttatgtaca aaatggtcta ttggcttcaa tccttctagt tagtaaggca gcggattttg
     1561 ttattacagg ttgtggcacg ggggtaggtg ccatgttggc ggctaatagt tttccagggg
     1621 tagtttgtgg ctttgcggta gatcctacgg atgcatacct cttttcacag ataaatggag
     1681 gaaatgctgt gtctctgcca tttgcaaagg gattcggttg gggagctgaa ttaaatctca
     1741 aattactttt tgagcgactg tttgcagagg aaatgggggg cggatatccc cgtgaacgcg
     1801 ttattccaga acaacgaaat gctcgtattt tgaatgaaat gaaagcagtc acctataaag
     1861 acttactgac agtacttcaa gaaattaatc aagatttttt gaaagaaact atttcaggtg
     1921 aacatttcca agaatatttc tttgccaact gtcaggatga gtcaattgca gcatatttaa
     1981 aaacagtatt agaaagttaa agagtgaggg gcggttatga agaaggtatt actgattggt
     2041 gaacctttaa ttcgcattac accaacaaat tatcaagaat tgacagatgg tgtagaaagt
     2101 cgtgtgtttt ttggaggttc agaaatcaat gtcgcttgta atcttcaagg atttggatgt
     2161 gaaaccaagc ttttgaccgc actaccacaa ggctctctag gtgatagatt tttagatttc
     2221 ttagacgaga aagggattga tacttccagt gttcaacgta agggggaacg tgtgggagta
     2281 tactacatgg aagcaggatt tggttgccgt tctagtcaag tctactatga tcggggtcag
     2341 tctagtttgg aagctattca agtcgatcat tttgatatgg atcaattgtt tttagaggtt
     2401 gggttggttc actttagtgg tatcaccgtg gctattggtt cagctgttag ggcatggctt
     2461 gttcaagtat taaaagaagc aaagcggcgt aaaattccga tttcgatgga tcttaattgg
     2521 cgttctaaga tgattggatt aagagatgca aaaaaagtgt tctcgcaatt tgcgacctat
     2581 gcagactatt gttttggtct ggaacctatt atggctaatg aaaaagatgg ggaattattt
     2641 gaaagagata cgaccactgt ccatacaata agagaacgga tgcaaacttt aaaggaagcc
     2701 tataagttcc aagcaatttt ccatacgatt aggcagacag atgaactagg cagaaatttg
     2761 tataaggctt acggattggc tgatgaattt tgttgctctc aagaattgaa aactcaggtg
     2821 ttacaacgtg ttggcagtgg tgatgcgttt gttgctgggg cattacataa attgttagag
     2881 gggacgtcgt taaaggctat tttagatttt gcagttgcta gcggaactta taaatgcagc
     2941 atagaagggg atcacatgcg gcaatctctt agaaaagttg agtctttatt agaggggaaa
     3001 aatgacatta tacgataaag gagaaaaaat gagtccatca caaattattt caaaattgaa
     3061 ggagcagggg cttgttatag tcgttagagg agatgacaaa gaagacggat ttaagacggc
     3121 acaggcctgt attgcaggag gaattacagc tatagaggtt gcttatacaa atcctgacgc
     3181 gaatatgatt attacagaac ttagtcgatg ccataaagat gatgtttctg ttttaattgg
     3241 ggctggaacg gttctagatt ctgtaacggc acggcaagcc attatggcag gagcaagtta
     3301 tattgtttca ccatctttta atatagaagt tgcaaaaatc tgtcatcttt atgcagtgcc
     3361 atatcttccg ggctgtatga ctttaacgga gattacaaat gctttggaag caggctgtga
     3421 aatggttaaa ctgtttcctg gtagtgtttt ggggacaaac tacattactg caatcaaagg
     3481 accattgccg catgtttcaa tcatggtaac cggtggagta aggcttaata atgtttcaga
     3541 atggtttaac gctggtgcaa ctgttcttgg tatcggtggg gaatttaatc aattagctac
     3601 taaaggagag ttcggtacaa ttcaagaatt agcgtcctct tatacaagtg ttagagataa
     3661 gatgaagtag tttatttcaa aataaggact gtatttttaa cgttttcact ttctcctgcc
     3721 tccaacgggg ttgaatgtgg gagaaagtga gatgttacag gggaaatttt gaacggaaac
     3781 atggagatat ttggtactcc tgtttatgaa aagagggagc tggagaatat tttttaaata
     3841 aaagtccaac aaaatataaa acgcttacat ttttttataa aatctattga caagtggttg
     3901 tcaatagtct acaatacaaa atgtaggtaa accggtaaag ttaaaaggag agaattatga
     3961 ttaaagtgat tgtcgttgct catggacagt ttgctagtgg gattcttact tcactaaagt
     4021 taattgcagg agaggtggag aatattgagg caattgattt tagtgaagga atgtcggcgc
     4081 aagaactaaa agcacgcatc aaatcagcta tattaggcga aagagaagtt ttgattttaa
     4141 ctgatttact aggaggtacg ccttttaaag ttagtgtaga attagcagca gaacagaaag
     4201 agcagaatgt tgtcgttctt tcaggtttaa atcttgcgat gattttggaa gcgaattttt
     4261 cacggttaac agatgattta gaacaattgg ttggtaaatt gattaccacc tctaaagatg
     4321 gaatcgttga ttcggtcagt ctgttagcag aggataacca tgatgaagag ttatttgaag
     4381 atggtatatg agggagaaat gcatgattaa ggatgttttg attgagtcaa ttgctaaaaa
     4441 aagagagttt agaaatttca ctctattgag taagaaagaa gttgaggctg caatagaatt
     4501 ggcgattcaa caacttgcta tcaattgtga atattttggt gagtcgttcc cgactccggc
     4561 cacatttgat aaccattatc caaagatgga taatacagag tggacaaatg gtttttggac
     4621 gggcgcgctt tggttaggac atgagtattc tttgaacaat gaaatgaaag cgttagcaaa
     4681 aaagaatacg tcatctttct tggaacgtgt caaacaaaga attgaattag atcatcatga
     4741 tttgggattt ctgtattcac catcaacggt agctgattat aaattgacag gtagtaaaga
     4801 ggctttagag gcttcgctct tggctgctga taaattaatg gagcgctatc aagaaaaagg
     4861 aggttttatt caggcctggg ggcaacttgg taaagcagaa gattatcggt tgattattga
     4921 ttgtttgctg aatttacaat tacttttctt tgcttatgag gagacaggtg agcagaaata
     4981 ttatgatgta gctcttagtc atttctacgc ttctgttaat acagttattc gagatgatgc
     5041 ttcaacattt catacctatt actttgaccc tgaaacagga aaaccgctca aaggggttac
     5101 ccgacaagga tttagtgatg actcttcctg ggcgcgtggt caatcttggg gtatttacgg
     5161 tattccgtta gcttaccgta aaattaaaga tcccgcatgt ttaaatttgt ttaaaggagt
     5221 aaccaactac tttcttaatc gtttgccaga ggataacgtt tcctattggg atttaatttt
     5281 taatgatggt agtgggcacg ctcgtgattc ttctgcgaca gcaatagcgg tttgtggtat
     5341 tcacgagatg cttaaatatt tgccagaagt tgatgaggac aaggaaactt ataaatatgc
     5401 tatgcacacg atgctacggt ccttgattga taactatgct aatcgtgagt tagctccagg
     5461 tcgtccattg cttttacatg gggtctactc ttggcattca ggaaaaggtg tcaatgaagg
     5521 aaatacttgg ggcgattatt attacttaga agctttgctt cgtttttata aagattggga
     5581 attatattgg taggaggtta aaatggcaac accaaatatt gtaatggctc gtattgatga
     5641 gcgactaatt catggacagg gacaactatg ggttaaatcc ttaggttgta atactgtcat
     5701 cgtggcaaat gataaggtta gtgaagataa gattcagcag acattaatga aaacggttat
     5761 tccagattca gtagctatgc gtttcttctc tttgcagaaa gtgattgatg tgattcataa
     5821 ggctaatcct gctcaaacta ttttcttagt tattaaagat ttgcaagatg cactaacttt
     5881 ggttaaaggg ggagtaccaa tcacagaatt aaatattgga aatattcata atgcgcctgg
     5941 aaaaacgcaa gtaacacggt ctatcttttt tggttcagaa gataaggcta tagtgagaga
     6001 gttgaacgat gtttataaga ttcgcttcaa cacaaagaca acgccttctg gtaatgatgg
     6061 ggcagttgaa gttaatatat tggattatat ttaaaaaata aggagaatta tcatgacgat
     6121 cagtctaatt caagcagctc ttattggtct gtggacagct ttctgtttta gcggtctatt
     6181 attggggctt tacacaaacc gatgtatcgt gctttctttt ggggtcggaa tcattttagg
     6241 agatctacca acggctttag ctatgggtgc tatcagtgag ctggcataca tgggatttgg
     6301 tgttggtgct ggggggaccg ttcctccaaa tccaatcgga cctggtattt ttggtacttt
     6361 gatggctatt acaagtgctg gaaaagtaac accagaagca gctttagcac tttcaacacc
     6421 tattgcggta ggtattcaat tcttacaaac ttcagcatat actttacatg cgggtgctcc
     6481 agaatcagct atcaaacatc ttcgtgcagg aaacctttct aaatttaaac gtgttgcaaa
     6541 tggtacagtg attactttcg cctttttagg atttgctctt ggtttcttgg gtgcctattc
     6601 aatggatact cttttgaaat tggttgaaat gattccacca gtattattaa ccggattgag
     6661 tgtagcaggt aagatgcttc cagctatcgg ttttgcaatg attttgtctg taatggctaa
     6721 gaaagaattg atcccgtttg tattgcttgg ctatgtttgt gcagcttatc tacaactgcc
     6781 tgtaattggt atcgcaattg tcggtagtat ctttgcactt aaagaattct ttaataaacc
     6841 agaacaagtg gcagtgaaac aggaggaagc acacgatgac tggatctaaa ctaactaaaa
     6901 aagattatct caacacttct ttacgggcct tcttcttgca gaatggtttc aattacagta
     6961 attatcaagg gattggctat gcaaatgtca tgtatccagc ttttaagaag cattttggtg
     7021 acgacaaaga aggattaact aaagctttag aagaaaactg tgagttttac aataccaatc
     7081 cacattttct tccattcatt actagtcttc atttggtcat gttagaaaat gatctccccg
     7141 aagaagagac tcgtgggatt aagatggctc tgatggggcc gttggcagga attggtgact
     7201 cactttcaca atttgtatta gccccacttt tatctacgat tgctgcctct ctagcaggtc
     7261 aaggactggt tatgggaccg attcttttct tccttggaat gaacattatc ttgacctcta
     7321 tcaagttgtt gactggtttg tatggctata aatttggtac cagcgtgatt gataagttga
     7381 gtgaccaaat ggcaacaatt tctaaggtag ccaatatcat cggtgtgaca gttatctctg
     7441 gtttagcagt aacctttgta aaaatttcgg tgccaattac ctttgcggcg ggtgaattaa
     7501 cagaaggagc agatcagaaa attgtaacca tccagggaat gttagacaag attgcccctg
     7561 cgttgcttcc agccttgttt acgattttgg tttattatct catcaagaac aagaaatgga
     7621 ctacctataa attggttatc ttgacagtta tcatcggtat tttgggtagc tggttgggca
     7681 tccttgcata agcaattatg gatcaacaaa cactctacta tgcactaatt attttagtag
     7741 ctctatcgac catacttcct gtacttacgt cttatgtcaa tcgtaagcga caacaaaaag
     7801 cagttgagga gcaactggaa aaacgccaac gctacttatc aaccttacag gctggagatg
     7861 aggttatcct cttctctgga ctgcatggta aaattttcaa actaacagat agcttggtag
     7921 aactacagat tgcaaaaggc attattgttt acgttgaaaa agagtcgatt atgggaaaag
     7981 ccaaagaact actttttgcg aagtagttct tctttcttta agagagaaaa gcgattgcga
     8041 aataaaaaat gttgtagtaa cattgtttta cactgtataa aaaggaaagg aaataccaat
     8101 ggggattttt accagtcaaa cgaagcgata ttatagcatt cgtaaatata aaattggagt
     8161 ttgttcagct cttattgcgc tgtctatatt gggaacaaga gttgcagcca atcagttgcc
     8221 aagtacagaa acagctagtc cacaaactag tcagttagtg gaaacaactc cagaaacggc
     8281 agaaacagcc actcttacga cgacaagtga ggtcagctct gaagctagtc ctattacgtc
     8341 aacagaaagc cagccttcaa gcaccactgc agaaactctt gccagtccgc aagctgttca
     8401 ggcgacaaaa gaagagaaga acctagtagc taatggggag ttcatcagta caacggcacc
     8461 atcaggaaac tggaaagaat cggctgctac agactgggaa acgtggattc cgaatgagat
     8521 taaaaaagaa aatggagctg tttctattga tgatgggaaa cttcatattt cttcaacaaa
     8581 tacttaccga gtggcagtgc accaaacagt ggatgtagat cctagcaagc gctacctctt
     8641 tagctatgat gtcgaaacca aggatctgaa ggggagtggt gttcgtgttc gcttgcgcag
     8701 tttgactgca gaaggtaagg atttgagccc gcaagaattt actttcacaa attttgtggc
     8761 tggtacacaa caaagaaagg tggagaagga gttaacagtt tctcccgaaa ctcgtaaatt
     8821 gaaggtagag ctgttttttg agaatggtat aggccaagcc tggctggata acatctcgct
     8881 ggtcgaatac gtagaaaaga caccagaaac accagagcca agcccagaac ttgtccaacc
     8941 tgaaacaggg caaataagtc ttgcaagcaa caaggtctac ctgcctgttc ggccagacct
     9001 gacctatcag attgcggatg cggctgtggc cacagtagag aaaatatgga ttcgtccgct
     9061 tgcggctgga aagactcagg tagatgttta tgataaagac accaaactat ctagtttcga
     9121 gctgacagtt acggagcatc aagcaacagt ctttgatacc ttaaggaaca actgggaaga
     9181 tatttcttta gccaataaac gctaccaatc taacgatact cagatgaaag cctttctagg
     9241 gcgtttagat gccggcgtag cttcttctct taaaaaatgg gttgaaccaa cagatcaggg
     9301 caaaacgatt ttcaacgata ttgactttag taaatccagc catctgacaa ctgtctatcg
     9361 ccgcttggag cagatggctc aagtagttga gaatccagac tcagcctatt accacgaccg
     9421 ctcacttatc gatttggtca gaaaaggaat gaactggctt tacaccaatg tttataatga
     9481 aaataagtca attgatggga attggtggga ttatgaaatt ggaactccgc gtgcggttgt
     9541 caacaccttg atctacatgc atccctactt tagtcaggag gagattctaa cctataccaa
     9601 accgatttca aaatttgtac cagatccaac cactatccgt aaaacattga ccaatccagt
     9661 cccagcagtt ggcggtaatc aaacggattt gagcaaggtg gccatcttgg aaggtgctct
     9721 tcgcgaagat gctgaccgtg tgagggctgg tgcacaaggt ttgacaacga taatgaaatt
     9781 tgtagataag ggagagggat tctacaggga tggctccttt atcgaccaca ccaatgttgc
     9841 ctacacaggg gcgtacggaa atgtcctcat tgaaggtttt tcacagttac taccagttat
     9901 tcagccaacg gagtttgccc tcaaggaaga acagaccaac attctttatg aatggattga
     9961 aaaagccttt atgccaatat tggttcgcgg tgaactgatg gatatgaccc gcggtcgttc
    10021 catcagtcgg gcaactggtg aatcacatgt tcaggccatg gagattttaa ggtctttggt
    10081 ccgcattgca gaatcagctc agcctgagca aaaaaccaaa cttctctcct ttgttaaggc
    10141 acagctgacc tctgatacct tctatgacag ctaccgcagc ctcaaatcct ataaggatat
    10201 tgatttggtc aacaagcttt tagctgataa tcaaatccct gttgaagtgg ataaagatta
    10261 tattgcagcc ttcaacaata tggataagtt tgtttatcga agcgctcaag aaggctttac
    10321 ctttgccttg tctatgtact cttctagaac tcaaaactat gaagatatga acaatgaaaa
    10381 ccgcaagggt tggtatacag cagatggtat ggtttacctc tacaatgatg atttgagtca
    10441 ttacagtaac cactactggg caacagttga cccgtatcgc ttaccgggaa cgacaacgac
    10501 caaggacaaa cgagaagatg gcagtggcga ggtaactctt gcaagtgact ttgttggagc
    10561 aagccagtta ggcaaccgct tagcgacgat tgcaatggat ttcaacaact ggaacaatag
    10621 tctgactgct cgtaaggctt ggattgttct gggaaataag attgttttcc taggtactga
    10681 tatacagcat caatctgctc aaggcgccga gacaacgatt gaaaaccgta aactcttgac
    10741 cggagaaaaa tatagctatt acataaatgg ccagccagtt gatttgtcca aagaagtagt
    10801 gacagataag actcaatcct tttacatgac caatggcagg gacaaccagt ccatcggtta
    10861 cgtcttcttg aaccaattgc cgactcatgc caagttagat cagcgaaccg gcaaatggag
    10921 cgacatcaac tacaaccagt cgaaagaaga agtgagcaat tcctttgtta gcctatggca
    10981 tgaacatgca cagacttcaa gtaactatgc ttacgtcctt gttccaaacc aatctatgga
    11041 aaaagtgaat caggcagctg ctagtgtgaa attgcttcat caagacaggg atttgcaagt
    11101 ggtatacgac caagaacaga atgtctgggg cgttgtgaaa tacacggata cagcctacaa
    11161 gctgacagat gatattacct taacagatgc cggtttgtat accattcaga aagtcgaagg
    11221 tggctatcgc atagcctttt acaatccgtc tacccgaact gtaaagaatg gcattgagtt
    11281 gacaaaggca ggttccagtc tgactgttga gatggagcca accgcagctt gtccatccac
    11341 tgtctggaag gttactatgc cagaaggttc tgataaacaa actggaagta tcgaaaaaac
    11401 agaaaaagaa gaaaaacaac tcaaggaaaa ccaaccaagt ccagaggtga aacaagtggt
    11461 tcatcatgct gccgaaaaaa caaagccgag taagccccgt ctgccccaga caggagaaga
    11521 agcaagttta ggtcttggtt tcttaggttt gctaaccttg ggggcagtgg tggatttcaa
    11581 gtgtcgcaga tcgcgctcct aattgaaatt cgtgggtgaa gggggagcgt gaaagaactc
    11641 gactagtcaa aaaagagttc gtcaacaagt ctttatttct agttgttgag ctgaaacagt
    11701 ctatccccag actgtttcac tccacccctg catagttgat tagatcagat tcggagtatg
    11761 acatacgaat tgctgaaatt tgttttggaa ctcctatctc caacctttaa cagtccaccg
    11821 aactgttaaa gccgatcaat cactgcgctg agatgttgat atgaactttg aggagtggat
    11881 ttgggctttg cccagcctct cttcttttgg agaagcgata gagaaaggac ttgttatgca
    11941 aacaatattc ggaaaaatag tagccaatca gtcagcagag tcggtcaaaa cctatattct
    12001 caatcaacaa gatgcagctt ataaagagct tctcctttct tgtcaaaatt tattaaatga
    12061 tatttttatc tttgaagaca attgggatat ggaaccttgt tcagtcgggt atcatcttgc
    12121 tcctcttgac tggacagccg actgccatag ggatgaggaa tggaccttta tgctcaatcg
    12181 gcaggaatac ttgtggaaat tagttttggc ctatctggtt gaaaaagatt cgcgctacat
    12241 ggagcaggtt aaacggttta tctttagttg gatagaacaa gtgacagatt ggcatcctaa
    12301 aagaacgagc agtcgcacct tagatacggc tatccgctgt ctaagctggc tcaatattct
    12361 cccattttta attgattggg gcggactaag tgaagctgag cgtagccaac tgttagtatc
    12421 cattcaaaaa caattatctt accttcacag tcattaccgt gccaaagata cgttgagtaa
    12481 ttggggaatt ttccagactg gcgcaatgct cttggcccat gcttatatcg gtgatcaggt
    12541 ggatttagta gagcttcacc attttgctcg tcaggaaatc caagagcaaa ttcaaactca
    12601 gatattggaa gatggaactc agtttgagca gtcttttctc taccatgtgg aagtttataa
    12661 actcttgcta ggactagctt tcggcttgcc agattatcga aaacagtgga cacctatttt
    12721 gaaaaaaatg gcagactatg tcttacagat gacggggcca gatggcaaaa gtgtggctct
    12781 tggcgatagc gatgttcatt gtacttctga catcttacag ttgactgcga ttttctttga
    12841 agatggtacc tacctagtag ataattccaa gctagattta ggcgttctct tcttggtcgg
    12901 agaaaatgga ttgcgcatat ttgaacactt gaccgctcaa cccagttcgt tagggggaca
    12961 gcatttttct gaatcaggtc acagtattgt caaagaagtg gacagctatt tatgtttcaa
    13021 ggccggtccc atgggaagtg cccacagcca ctctgaccaa aatagcttgt gcttttacca
    13081 caaaggacaa ccaatcgtaa ttgacagcgg tcgtttcagc taccgtgaat gcgaggagcg
    13141 ctacttgctt aaaagtgctt gggctcacag cacttgtatc cttgatgaca atgctccaga
    13201 gcagataaag ggttcatggg aatatgatac gtatcctcag tttattagtc acagttataa
    13261 gggttttgga cgctgtcatt ggatgcaggg catttatcag gctcagacca gccaaggctt
    13321 gccttactgg cataaacgac agctccttat ggttggaggc gatttattgc tgatagtgga
    13381 tagcgttgat tgctcaggcc agcatgagtt gaccagtcaa ttgattttag atgaaagggt
    13441 caagattgga gaagagcagt taaatgatct tcggctatat agtcaaaagg attttgaaat
    13501 ccagaaaacc gtgcgttctc ctcgatacaa ccaactagta gagaccaata aactggtcaa
    13561 aaaagagagt tttcgagatc gcttagttac atttactcta ttagcagatg aagcgattcg
    13621 tgtcaagcaa ttgccagttt accaaacaga ctatcatttg atagaaagtg gtttggcttt
    13681 tagctgtcaa ggaaagggac tgaatattct gttagtggtt tctgataatg atatatataa
    13741 gggagagaaa ttgcttcaag tggctggtta taagttgaga ggtaagtgct tagtagtgga
    13801 tagggataag agacaagttg tccgactcaa aaattagaag ggacagaaaa aaatacagtc
    13861 atttctgtgc aaaataactg ataaagtacg gatagtatga tagaataaag taactaaagg
    13921 aggtatcggt aaggtgtcag agaagtttac catcaaagat attgcagaac tggctcagac
    13981 ttcaaaaaca acagtttcct tttacttaaa tggtaaatat gaaaagatgt ccaaagagac
    14041 acgcgaaaga attgagagag tcattcgcga aacagactat aaacccagcg ttgttgcgcg
    14101 gagcctaaat tcaaaaaaga ctaagttgat tggagtattg attggtgata ttacaaatac
    14161 attttccaat caaattgtca aaggtataga agaagaagcg cataagagtg gctatcaagt
    14221 tatcatcgga aacagcaatt acaatcaaga aagcgaagat aagtatattg agagcatgct
    14281 gatgttgggt gtagatggtt ttattattca gccgacatcg cattttagaa aatattcgcg
    14341 cattattgag gataagaaaa agcatatggt tttctttgat agtcagcttt acgaacaccg
    14401 taccagttgg attaagacaa ataattatga tgctgtttat gatgcaactc aactatgtgt
    14461 agaaaatggt tatcaaaaat ttattcttat tacagctgac attagtcgct taagtacacg
    14521 gattgaacga gctacagggt ttatggatgc tctgttagat tgtaaacaaa gttatcaaga
    14581 tttaacaatt gcagatgaaa atacggacat agaggaaatt aaacgttttc ttcaaggggt
    14641 ggtaaatcct caggaaaaga ccctagtatt cgttccgaat tgctgggcgt tgcctttagt
    14701 ttttaatgct atgaaagaac ttaaatatga ttttcctcag gttggtcttt tggggtttga
    14761 caatactgaa tggacaaatt tttcatcccc tactatctct accattgttc aacccgcctt
    14821 tgaagaaggg tgtcaggcaa caaggatgct gatcaatcag attgagggcg gaaaccaagt
    14881 gggacgacaa caggttttag attgcacagt tcattggaaa gaatctacat tctaagttaa
    14941 gtcaaatttt acttcttagg aagaggagca gaacgaagta gaatattatg attgagagct
    15001 cactgaaacc ctccttgaat cgcctaatat atacaaaaaa taggaatttt cccttaacta
    15061 gtgggagaat tcctattttt atgccaatct cattgagatg atatattaat attatatatg
    15121 attttaaaat caacagattt ctatagtctt ttagtactag gcaacgagct gcagacagta
    15181 ctgtggagta cggcaaagcg agttcaaaca cttcggtgga gtgtttgaag ttggaaataa
    15241 gggagtggga agacgaactc taagaagtgg cgctgggctt tttgcccagc ctcacgtcaa
    15301 agataaacaa aatgactata aaacggaaaa atagaaatga tatttttcct aagatttaca
    15361 acccatataa ataatcatca tctgtcatag cttccacttc accaagaaga tagccgttac
    15421 cgacttggga gaagaagtcg tggttagatg taccggttga gataccgttc atgatgattg
    15481 ggttgacatc atctgctgaa tctgggaaga gtggatcttg tcccaagttc atgagagctt
    15541 tgttggcatt gtaacgaagg aaagtcaaga cttcctcagt ccagccaact gcatcataga
    15601 gggagcgtgt atagccctct tcattttcgt aaagttggta gagcaggtcg tacatccagt
    15661 cgcgaagttt gtcctgttct tcctctgata attcattgaa accaagctgg aacttgtagc
    15721 cgatgtaggt accgtgcaca gattcgtcac ggataatcag tttgatgatt tctgcaacgt
    15781 tggccagttt gttattccca aggtagtaga gtggcgtgaa gaaaccagag tagaagagga
    15841 aggtttcaag gaatacgctg gctactttct tttcaagtgg tgtacctgtt tcgtagattt
    15901 cattgataat ctttgccttg cgttgcaaat attcgttgct gttggtccat tcaaagatgt
    15961 cttcaatctc agacttggta ttcaaggttg agaagataga agagtaagat ttggcgtgaa
    16021 cagactccat gaattgaata ttgttgtaaa cagcttcttc atggggtgtg cgaatgtcat
    16081 tgcggagggc ttggacacct gtttcagatt gaagggtatc caagagggtc aatccgccaa
    16141 agaccttacc aaccaggtct ttttcttcag cagtcagttt acgccagtca tccaagtcgt
    16201 ttgataatgg gatacgtgta tcgagccaga attgctcggt caacttttcc caagtagact
    16261 tgtcgataac atcctcaatg gcgttccagt ttatggcttt gtagtaggtt tccatcatat
    16321 tctctttctt tttcttatac ttcaaatatt atatcacaaa tagatagtag cctgtaaaaa
    16381 aatcccctaa aagggatttt aatcgattgc ttattagttg cttcacatcg tgaacgacag
    16441 tgttgtaagg tagctagcca atccgctcat agcggttggc gtagattagc tagttggctt
    16501 gtcaacagct agacttgtga aagttacgac ctgagaaaag tcgttcgaac ttaacgactt
    16561 ttcgaggtag taagccactt aggtactgac tatatggtca gtaccgttca ttcgttagat
    16621 gctttagcat caatgaatgt tagtgtcttg aaatcgagtt cgggctaagg acttggcaaa
    16681 aaagataaat ccctacgaga tgcaagccat ctctgtggta tttcctattt tctcatttcc
    16741 aactttcaac agtcccccag actgttgaaa cgccctcaca tcttacgaca cgtagaaact
    16801 gaagacgata tttcttcagt ttcgaagtta gtaaggtagc tagccaatcc gctcatagcg
    16861 gttggcgtag attaggtagt tggcttgcca acagtaaaac ttgtgaaagt cacgacctga
    16921 gaaaagtcgt tcgaacttaa cgacttttcg aggtagtaag ccacttaggt actgatcgga
    16981 taatcagtac cgttcattca ttagatgctt tagcatcaat gaatgttagt gtcttgaaat
    17041 cgagttcggg ctaaggactt ggcaaaaaag ataaatccct ttgagatgca agacatctct
    17101 gcgggatttc ctatttttgc ggtgtccttt tgacgccctc acatcttaga tcacacaact
    17161 ttcacattgg tttgcgccga cttcttcgcc gtcatcggta aaggtacgga cgtagtagat
    17221 agacttgaca cccttgttga aggcgtagtt acgaaggata gagaggtcac gtgtagtttg
    17281 tttgttctct tttttccatt catagagttc ttttgggagc tcgctgcgaa ggaaaagagt
    17341 gagtgacaaa ccttggtcga cgtgttctgt tgcagcagcg tagacatcaa tgaccttgcg
    17401 catatccata tcgtaggcag atttgtagaa tggaatggtt tctgttgcca agccagctgc
    17461 tggatagtag attttaccga ttttcttctc ttggcgttct tcaatccgtt gggtaatcgg
    17521 gtggatagaa gcagaaacat cattgatata agagatagaa ccgtttggtg caacagctag
    17581 acggttttga tgatagagac catctgccat tactttttca cgaagctccg cccaatcttg
    17641 tccacttgga atgaaaatgc cttcaaaaag ttctttgaca cggtcagatt gtggttggta
    17701 gtcgccagtc acgtatttat cgaaataagt gccgtctgcg tattttgatt tgtcaaaatt
    17761 atggaaggtt gccttgcgtt cacgggcaat gttatttgat tctgccaaag tccagtagtt
    17821 gaggagcatg aagtagatgt tggtaaattc tacggcagtt tttgaaccgt aatcaatcaa
    17881 gttctgagcc aagtatgagt gaagccccat agcaccaaga ccgattgaat gcgcacgttc
    17941 atttccagcc tcgatagatg gcaccgcaga aatgtgcgaa tggtcggtca cgtatgtaag
    18001 agcgcgtgtc atggtacgga cagaaagtcc gaagtcaggt gacttcatga ggttgacgat
    18061 gttggttgaa ccaaggttac atgatacatc tgtccccatg gtcaaatatt cctgagaatc
    18121 attgatgacg ctcggcgttt gaacttgaag aatttcagaa cagaggtttg acatgataat
    18181 cttgccgtca acagggttgc tacggttagc tgtatcgatg ttgataacat atggatagcc
    18241 agactcttgt tggagtttgg aaatttctgt ttccaagtca cgggccttga tttttgtctt
    18301 acgaatgcgt gggtttgcca ccaattcatc gtatttttct gtgatgtcaa ggtagctata
    18361 agggacaccg tattccaact caactgagta tgggctgaag aggtacatat cttcgttttt
    18421 acgtgccaat tcgtagaatt tatcaggaac ggtgataccg agtgataagg tcttgacgcg
    18481 aaccttctca tcggcatttt ccttcttcgt cgaaagaaag gcgataatat ctgggtggaa
    18541 aacatctagg taaacaaccc cagccccttg gcgttgccca agttggttag agtaggagaa
    18601 actgtcctca aagagtttca ttacaggaac gacaccagaa gcagctccct cgtagccctt
    18661 gattggtgca ccagcctcac gcaagttgct aagggaaata ccaacaccac caccgatacg
    18721 ggacaattgc agagcagagt tgatagaacg tccgatcgag ttcatatcgt cagtgacttg
    18781 aatgaggaaa cacgatacca actcaccacg acggtcacgg cctgcgttga ggaaagacgg
    18841 tgttgccggc tggtaacgca aatgaatcat ttcgttggcc aggtttagtg ccaattcttc
    18901 gtcgccctca gcaaaataca aggcgttgaa gagaacacgg tcttccatgc tttccaagta
    18961 gtaggcaccg tcgttggttt tgagggcata ctggttgtag aacttgtagg cagccatgaa
    19021 agacttgaag cggaaatttt ggtctttcag gaattgatca attttgatga taaattctgg
    19081 gctatactgg tccaaaaact ctttttcaag atagttttct tctaataaat aggcaatctt
    19141 gtctaaaata ctatcaaatt gcttggtatt tgggaggacg ttttctttaa agaaggcctt
    19201 aaccgcttct tggtccttgt ggagtgggat ttgcccatta acagggcggt tgatttcgtt
    19261 attcaaacgg aagtaggaca catcgcctaa ttctttcaaa ctcatagtcg caatctcttt
    19321 ctataaacag tagggagtta aaccaaggct tttagttttc ctggttgaaa acctgagaaa
    19381 acatcatttt cagtttcgat aacaggtgca gctgagaaac cgagcccttt tacgtgatcg
    19441 ataaattcag gctgttcatc tagattgatt tctttataat ctacactatg ttgatccaag
    19501 aatttcttgg tcattttaca ctgtacacaa tcattttttg aatagatcgt taccattcaa
    19561 tttctccttt gtctgtcatg gataaaaata tcccattttt gttaccaatc taggatacaa
    19621 aaatttgaag ttaaagtcaa gaaggaagtt tgtttaaaat cacaaaatat agtgcacaat
    19681 atttttacaa gacaccatat attgtacatc tgtccaatgc tgctgagtcg aaaaaaatag
    19741 cgtttacgta gctgtttcta tttagtttga taaccctaac aatctctcgg taaagtataa
    19801 atcagctttt ttacaaatgt cgtaatattt ttaaggaaat atacaatgtt acaaaatata
    19861 acatttatag tgatacatgt attgaatata tattaccttt tgtgctatac tattcaataa
    19921 tgccgaaaga ggttataaaa tatgggaaaa tcacatagaa aacgcttgtt tcataaaggg
    19981 aaagaatatt catattatgc attggattcg atttcaatgg aagaaaaggt ggatattcat
    20041 tcacttccct atactattcg tattttatta gagagtcttt taagaaaaga agatggtgta
    20101 gatgttacaa aaaatcacat aatggagttg cttcattatc aggctgcatc tcctaaaggg
    20161 gaaatccctt ttaaacctag tcgtgttatt ttgcaagatt ttacaggtgt tcctgttgtg
    20221 gtagatttag cctccatgcg tgatgcggtg gtgaaagcag gtggaaaccc tgaattgatt
    20281 aacccagaaa ttccagtgga tttagtcatt gaccactctg ttcaagttga tttttttggg
    20341 actgaagatg ctctggagaa aaatattgcc ttagagttcg aaagaaataa tgaacgctat
    20401 gaatttctga aatgggcaga gaattccttt gaaaattatc gcgctgtgcc tccagcaaca
    20461 ggaattatcc atcaggtaaa cattgaattt ctaagcgatg ttattatcaa caacgatggt
    20521 ttgctttatc cagattctat gtttggtaca gacagtcata cgaccatgat taacggtatt
    20581 ggtgtacttg gttggggtgt aggtggtatt gaggcggaag ctgcaatgtt gggtgaagca
    20641 tcttattttc ccgttcctga ggtgattggt gttcgcctag ctgggcaatt gcctaaggtt
    20701 gccactgcaa cggatttggc gcttaaggtc actcaacttc tacgtcagga aaatgtagtt
    20761 gggaagtttg tcgaattttt tggaccaggt ttatcttctc ttacattagc ggatcgagca
    20821 actgtttcca atatggctcc agaatacggt gcgacttgtg gctatttccc gattgatgga
    20881 gaaactttac attatatgag attgaccaat cgttcggagg agcacgtgga gttgacagaa
    20941 gtctatgcta aggctaatta tttattctat gatgctgaac gttttccgtc ttatagcaag
    21001 gttttagaat tggatttatc gacagtagtc ccatctattt ctggtcctaa aagaccacag
    21061 gatttgatag aattaacaga tgcaaaagca gagtttcagg ctagtttaat acgtgaagta
    21121 ggtgtgcgtg gatttggttt ggaggaagca gagttggata aaacagccac ggttaagtat
    21181 gttgaaggag atgagcaaat tcaaacaggt catgttgcta ttgcggcgat tacctcgtgt
    21241 acaaatacct ccaatccgta tgtccttttg gcggcaggat tactggcaaa aaatgcggta
    21301 gaaaaaggtt tggcagtttc taagacggtc aagacgtcat tggcaccagg ctcaaaagta
    21361 gtgactggtt atttgaaaaa atcaggtttg cagacctatc tagatgcctt agggttcaat
    21421 ttggttggtt atggttgtac aacttgtatc ggtaactcag gtgatctgcg tcctgaagta
    21481 gcagaggcga ttaaggaaga agatttattg gtttctgcgg tattatctgg aaatcgtaac
    21541 tttgaaggac ggattaatcc attggttaaa gctaatttct tagcgagccc accgcttgtt
    21601 gttgcctatg ctattgcggg aaatatgaat gtagatttaa caagggatcc gctggggtat
    21661 gatgagaagc aacaagctgt ctaccttgcg gacattatgc caagtcgtga ggaggtagat
    21721 gactacattg agcgttatgt gactcgtgac ctttataagg aagaatacca acaggtattt
    21781 atagatagtc aggcttggaa tgcgattgaa acaaaaacag ataagaatta caattggaat
    21841 tcatcttcta catatattca aaatccgccc tattttgaca atatgcaggc agatttatcc
    21901 ataaaaccac tggaaaattt atctgttttg gctaaatttg gcgattctgt aacgacagat
    21961 catatttccc cggcagggaa tattgcacga ttgagtcctg cagctcgtta tctcgaagag
    22021 aatggcattg tttataagga ttttaattct tatggaagtc gtcgtggaaa tcacgaagtc
    22081 atgatgcgtg ggacttttgc aaatattcgt ataaagaatg agctggcaga tggaaaaatt
    22141 ggtggttgga caagagttgc cgatgagctt cttcctatct atgatgcggc tatgaaatat
    22201 aaggaagctg gtgtcggtag tattgttatt gctggtaaag actatgggat ggggtcaagc
    22261 cgtgattggg cagccaaagg ttcaagttta cttggtgtga aggctgttct tgctgagtca
    22321 tttgagcgga ttcaccgttc aaacttagtg atgatgggtg ttcttccttt acaattttta
    22381 gaaggtcagt cggcagaaag tcttggcttg actggacatg aaagctatac aattgatttg
    22441 ccagaagatg tcggtgttgg tcaaattgta acagtccatg cgcaaacaga cgatgttaca
    22501 aaggagtttc aagcactggt gcggtttgat gcagaagcgg atattcgtta ttatcgctac
    22561 ggaggtattt tacctatggt agtaagaaag aaactaggag gaaaggtatg acagaaacga
    22621 atgggttaaa agatgcgatt gcctgtgata cgcgaatcag tgcaattgaa aatgaccgct
    22681 tgtcctatgc agggtatgac attgctgaat tgatggagaa taacgcttct tttgaggaag
    22741 tcatctatct tctgtggaac ctgcatttgc caactcagat tgaattaaat tattttgaga
    22801 aaagcctgcg aaaagaatat gcgatttcag atgcagtaga gcaatgtatt ctgattcaat
    22861 cacggcgaca tttgcatcct atgagcgtgt tgcggtcgac agtatctctc cttggagtat
    22921 ataatgtaca cgctgaagag aattcgttgg aagctttgta tgaacaatct attcagatta
    22981 tggccaagat tccgacgatt attgcaacat ttgctcagtt gagacgcgga ttggttccaa
    23041 ttgaaccgcg gtctgatctg ggttttgcgg cgaattttct ttatatgctg aatggtgagg
    23101 agccgagtga tttgcaaatc aaagcgttta acaaagcttt ggtgcttcat gctgatcatg
    23161 aattgaatgc gtctactttt gctgcgcgtg tcacggcatc aacgttaacg gatttatatt
    23221 cttgtgttac cacggcaatt gggactctga aaggctcatt gcatggtggt gcaaatgaac
    23281 gagtgtttga catgttgctg gctattcgtg agagtggtga taccgagaaa tatttgcaga
    23341 agaagctgga ttcgaaggaa aaaattatgg gatttggcca tcgagtct
//
LOCUS       DATUCG010000019        22956 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.020, whole genome shotgun sequence.
ACCESSION   DATUCG010000019 DATUCG010000000
VERSION     DATUCG010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22956
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.020"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            87..1085
                     /locus_tag="VCA20_000616"
     CDS             87..1085
                     /locus_tag="VCA20_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004192896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEP1834471.1"
                     /translation="MKVLLYLEGKTVLEKSGIGRALQHQMEALDMAGIPYTTDLLGDY
                     DVIHINTYGPRSWLLLHAAKRSGKKVIMHGHSTKEDFQNSFIGSNTLAPFVGKYLASM
                     YQKADFVITPSEYSKKLIQSYGVTTPIIAVSNGIDLDKYKKDERKEAIFREHFKIEEG
                     QPVVVCAGLYFRRKGIEDFVKVAEKMPHVRFIWLGSINKWLIPSYIRKIVEGDHPANV
                     EFPGYFKGAVFQGAMSGADAFFFPSYEETEGIVVLEALASHQHVVLRDIPVYDGWIDE
                     NVAELGHTVDDFVDSIQKILDKQVDKREAGYQVAVSRSIDNVSHQLVDAYRQVLEM"
     gene            1086..2399
                     /locus_tag="VCA20_000617"
     CDS             1086..2399
                     /locus_tag="VCA20_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEP1834472.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKTELEKLGHTVFIFTTTDED
                     VNRYEDWDIIRIPSVPFFAFKDRRVAYAGFTDTLKIASRYKLDIIHTHTEFSLGILGK
                     MIAKELDIPVVHTYHTQYEDYVHYIAKGRIIRPGMVKYLVRAFLKDLDGVICPSEIVE
                     DLLMSYNVPISKRVIPTGIDLVKFDRPEIGKDETDELREQLGIASDETMLLSLSRISH
                     EKNIQAIVQAMPKILLENPKVKLVIVGGGPYTDDLQEMIAELNLDEHVQLTGMIAPSD
                     TALYYKAADFFISASTSETQGLTFLESLASGTPILAHSNPYLKNVITDKMFGTLFIRE
                     QELADAVVEAIVATPPMNPYVLDKKLYEISSTNFGRKVFEYYLDLKISYDFRKEHTNQ
                     DSVAEALIKEAIYLPQKVVVKSTDTTAKILKKSVEQVKSIRNFFE"
     gene            2414..2788
                     /locus_tag="VCA20_000618"
     CDS             2414..2788
                     /locus_tag="VCA20_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834473.1"
                     /translation="MERLQMESWKSLVLATLIVLSMYAGIYLIAFHLISDTMLSKELV
                     VGNILLYTPIVLFLTGLLYGFFHGFSPTLLALILLLYPVTIFFFKEWIILYQIVYTLC
                     ALIGNGVGALLFSLRKKMRKNP"
     gene            3221..3406
                     /locus_tag="VCA20_000619"
     CDS             3221..3406
                     /locus_tag="VCA20_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HEP1834474.1"
                     /translation="MNRLIMTKQGRYYDETPYTLEHKMAENIWWLIELADRLDIDIQK
                     EMETFLAQKEELLGIKK"
     gene            3556..5505
                     /gene="thrS"
                     /locus_tag="VCA20_000620"
     CDS             3556..5505
                     /gene="thrS"
                     /locus_tag="VCA20_000620"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018164389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEP1834475.1"
                     /translation="MLKITFPDGAVREYQAGVTTFEIAESISKSLAKKALAGKFNGKL
                     IDTTRAIEEDGTLEIVTPDHEDALDILRHSAAHLFAQAARRLFPDIKLGVGPAIQDGF
                     YYDTDNAAGQISNEDLPRIQEEMMKIVKENFPSERREVTKEEALEIFKNDPYKLELIH
                     EHSDDEGGLTIYTQGEYVDLCRGPHVPSTGRIQIFELVNVAGAYWRGKSENPMMQRVY
                     GTAWFDKKDLKAYLQMREEAKERDHRKLGKELDLFMISQEVGQGLPFWLPNGATIRRT
                     LERYITDKELASGYQHVYTPPLASVELYKTSGHWEHYSEDMFPTMDMGDGEEFVLRPM
                     NCPHHIQVYKNHVRSYRELPVRIAELGMMHRYEKSGALTGLQRVREMTLNDGHIFVTP
                     EQIQEEFKKALQLIIDVYADFNLNDYRFRLSYRDPEDKEKYYDNDEMWENAQRMLKGA
                     MDEMGVDYFEAEGEAAFYGPKLDIQVKTALGNEETLSTIQLDFLLPERFGLTYIGADG
                     EEHRPVMIHRGVISTMERFTAILIETYKGAFPTWLAPTQVTMIPISVEAHLDYAWKVA
                     KELQDRGVRVHVDERNEKMQYKIRQSQTSKIPYQLIVGDKEMEDNAVNVRRYGSKATQ
                     TQSVSEFVDHILADIARKSRPAETE"
     gene            complement(5589..6632)
                     /locus_tag="VCA20_000621"
     CDS             complement(5589..6632)
                     /locus_tag="VCA20_000621"
                     /inference="COORDINATES: protein motif:HMM:NF013884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEP1834476.1"
                     /translation="MKRLIYIDQLKGFGILAVLFLHTVGYIRNALPYTDIPLWRLEAW
                     LHSFLMPFFVFVSGFSFYHFYLKPKPRNNTYIYIYIFRLLQIYAVFGSSIVISKILFS
                     SYTVDKIQPSHLVSNFLLPDTSMWYIWFLILMATSTLILHRLQLTKPVSLICIGLFTL
                     FAVLWGGPLNISNLPIGLQHLTSLPLFYFLGIYFAKKEIHKKMVRQTVFHSLAGLVTI
                     FYLIYLQSYQTYPSQHLPFHTFLRYANGLAMTYLIVSIIITLERRLPAVISWFGQCSL
                     YFYLIHTYILTILTLFIKKYFLDFPTPVLLLISFGLLLIINSLVLMIVKRIPILDKVF
                     QGKVISYPQKKSN"
     gene            complement(6755..7414)
                     /locus_tag="VCA20_000622"
     CDS             complement(6755..7414)
                     /locus_tag="VCA20_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009659320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEP1834477.1"
                     /translation="MNYVLELLKPSTFQLLWSGLKLTLYISSISVILSILFGMILAIM
                     RNGKNPIFKWTATIYIEFVRNVPNLLWIFTVFLVFQMKSTPAGITAFTIFTTAAMAEI
                     IRGGLNAIGPGQTEAGLSQGFTSVQIMVYIIMPQAIRKMLPAIISQIVTVIKDTSFLY
                     SVIALQELFGSSQILMGRYFEAEQVFALYLMVALIYFSINFAISSLSRYVAKSWASSI
                     E"
     gene            complement(7429..8127)
                     /locus_tag="VCA20_000623"
     CDS             complement(7429..8127)
                     /locus_tag="VCA20_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEP1834478.1"
                     /translation="MTVLTTSPYAWENWVSYFKDFPTFCQGFLFTLAISVGAFIMAMF
                     LGIVFGSLSSSKNKVLKIIARVYVEYYQNTPLLVQFMIVYYGLPLISNYVLMPSIYWT
                     AVICVGLYHGAYIAEVIRAGIEAVPTGQTEAALSQGFTQSETMRIIILPQALPTILPP
                     LTNQVVNLIKNTATVAIISGADIMFMTKSWSAMNANYIPAFAGAAFLYFIMCFPVASW
                     GRRIEEKNKSAFSH"
     gene            complement(8142..8981)
                     /locus_tag="VCA20_000624"
     CDS             complement(8142..8981)
                     /locus_tag="VCA20_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEP1834479.1"
                     /translation="MDKKKINIFFAIILLAFGLILLVFAKQPVAQTETPASITNSDQV
                     QAIIDRGVLRVGVKQDVPNFGYKNPDTGEFEGMEIDIARKIADELGVEIEFTPVTAQT
                     RGPLLDNGQVDMVIATFTITEERKLLYNFTTPYYTDAVGFLVNKDSGIKTFTDLDGKT
                     IGVAQGSITRTLISELADKYGISVNFAELGSYPELSVSLRAHRTDAFSVDQSILSGYI
                     SSKSELMDFSFSASDYGIVTKLSNKDLNDYLNSLVEKWTADGSLQSIYDANGLKPVTE
                     TDE"
     gene            complement(8992..9753)
                     /locus_tag="VCA20_000625"
     CDS             complement(8992..9753)
                     /locus_tag="VCA20_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEP1834480.1"
                     /translation="MPLVEFKDVNKYYGDYHALRNINLEFEPGQVVVLLGPSGSGKST
                     LIRTINGLEAIDEGTLIVNGHDISSTSIKELVSLRKEVGMVFQHFNLYPHKTVLENVT
                     LAPIKVLGINKATAEKTAQKYLEFVNLWDRKDSYPAMLSGGQKQRVAIARGLAMKPEL
                     LLFDEPTSALDPETIGDVLAVMQKLARDGMNMIVVTHEMGFAREVADRIIFMAEGEVL
                     VDTTDVNGFFDNPTEPRAQQFLSKIINHESDKVKL"
     gene            9947..10651
                     /locus_tag="VCA20_000626"
     CDS             9947..10651
                     /locus_tag="VCA20_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEP1834481.1"
                     /translation="MKKILIVDDEKPISDIIKFNMTREGYEVVTAFDGREALEVFEAE
                     FPDIVILDLMLPELDGLEVARTIRKTSNVPILMLSAKDSEFDKVIGLEIGADDYVTKP
                     FSNRELQARVKALLRRSELAETQTNLESTGTPELVIGDLVILPDAFVAKKHGKELELT
                     HREFELLHHLAKHLGQVMTREHLLETVWGYDYFGDVRTVDVTIRRLREKIEDTPSRPE
                     YILTRRGVGYFIKGND"
     gene            10644..11993
                     /gene="vicK"
                     /locus_tag="VCA20_000627"
     CDS             10644..11993
                     /gene="vicK"
                     /locus_tag="VCA20_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HEP1834482.1"
                     /translation="MINQLRYLMTTAEFWFVVILIGFLIALTVLLIENYRDNKQIKQL
                     NQKVNALIEGNYADVLDMRGSPEITDMANSLNDLSEVIRLTHDNLEQEKTRLTSILSY
                     MSDGVIATDRIGRIIMINDMAQKQLGLSNPKQEQYHLLEVLDLSDRYTLRDLLAQTPE
                     IVIDHTNENEEFLTLRANFATIRSESGLISGLVVVLHDMTEQAKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALTESVAPSFVKVSLDETNRMMRMITDLLSLSRIDNQVG
                     QIDVELINFTAFVTFILNRFDQMKNADSDKVYTIVRDYQISPIWVEIDTDKMTQVLDN
                     ILNNAIKYSPDGGTITFSMKTTDSQLIVSVSDEGLGIPKADLPRIFDRFYRVDKARSR
                     AQGGTGLGLAIAKEIVKQHKGFIWAKSEYGHGSTFTIVLPYSKDIALDEWDDSDEEE"
     gene            12000..12803
                     /locus_tag="VCA20_000628"
     CDS             12000..12803
                     /locus_tag="VCA20_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEP1834483.1"
                     /translation="MIGKGFNYSILASGSSGNCFYLETDKKKILVDAGLSGKKITSLL
                     AEIDRKPEDIDAILVTHEHSDHIHGIGVLARKYGMDIYANELTWQAMESKLGKIDVAQ
                     KHIFELGAMKTFGDLDIESFGVSHDAACPQFYRFMKDDKSFVMLTDTGYVSDRMVGIV
                     ENADAYLIESNHDIEILRSGSYSWNLKQRILSDKGHLCNEDGADAMIRSLGNRTKKIY
                     LGHLSKENNIKELAHMTMVNQLAQADLGVGVDFQVYDTSPDTATPLTKI"
     gene            13229..14545
                     /locus_tag="VCA20_000629"
     CDS             13229..14545
                     /locus_tag="VCA20_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEP1834484.1"
                     /translation="MSLQAGQKVGLVGNNGVGKSTFLKILLGLDRDFAGQIEVKADWA
                     YVPQLQERSSLSGGEQVWKSIQEAFAQRPQLLIMDEPTANLDQEHQEKLIKQIKRYRG
                     SLLVVSHDRHFLNQIASHIWHLEEGSIQVYTGNYEAFVESRRARREGQQQAYEAYQKK
                     VAQLKKAQHERQAKAQKMNKKKKGISSSEWKVNAMMGSYDSQAKSIAKSAKQLEKRIE
                     RLEKVSQPRKEAHIKMESKGALDTDLHRLFLLQAGQVLMDEVYLFDFPQIGMTFGEKL
                     ALVGANGSGKTSFVRKLISKELEGYYNPKLKIAYFAQDLINLDEEKTALENVSCTSLQ
                     DRMTILNLLGMLGLSYDKAQQKVANLSGGERVRLSLAKVLLADANLLILDEPTNFLDM
                     AAIEALETFLKEYEGSVLLVSHDQQFVETSVHSQWEIVQACLVKKS"
     gene            14637..16712
                     /locus_tag="VCA20_000630"
     CDS             14637..16712
                     /locus_tag="VCA20_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEP1834485.1"
                     /translation="MKKRKNKPLEHDGNFISLRLNILFSIVIFLFLVLILRLADMQII
                     NHDFYSNKLSTASQKIISNGSIRGQIYDAQGKPLVENEIQQVVSFTRPNKMSAQEMKE
                     VANKLLQWVNVSDVTITQRDKADYYLADEEVYRQVVEALPEDKKYDSDGNYLSESTIY
                     SNAVDSIPAEALQYSEQDSKAIELFKQMNGATYFSTVNLVTDTLTAEQVAHIVANESQ
                     LPGISTTNNWQRTILPTSLSSIIGTVTSEQAGLPAEDAEYYLSKGYSSNDRVGTAYLE
                     KQYEEVLQGQREKKQINLDRNGNVESIETIQEGQQGNNIKLTIDLAFQDGVNAILKKY
                     FESELATGSALYSEGVYAVALEPSTGAVLAMSGYSHEKGAGSITEDALGTITSVFTPG
                     SIVKGATISSGWENGVISGNQVQLDEPIYFAGSAPITSWYGAYGSFNIDATEALEYSS
                     NVYMVKIALGLLGQTYSANMYLNDGDTLTNAMTKLRSTFAEYGLGASTGIDLPLESTG
                     FLPEEYSTANFITNAFGQFDNYTPMQMAQYAATIANNGTRISPHLVEGIYGNNAQGGL
                     GELIETVSGKEMNQVNISAEDMSLLRQGFYQVVNGSGRFNTGSAIGRGAAVTISAKTG
                     TAETYTTTASGDVVTAVNTNVVAYAPSDNPQIAVAVVLPNLTNQNSTTTKTITQEIIN
                     LYQSLHPMN"
     gene            16721..17317
                     /gene="recR"
                     /locus_tag="VCA20_000631"
     CDS             16721..17317
                     /gene="recR"
                     /locus_tag="VCA20_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEP1834486.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMEDDVVNEFA
                     KNLLAAKRDLSYCSICGNLTDQDPCAICQDSTRDQSTILIVEDSRDVTALENIQEYHG
                     LYHVLHGLISPMNGIGPDDINLKTLLTRLMDNEVTEVIVATNATADGEATSMYISRVL
                     KPAGIKVTRLARGLAVGSDIEYADEVTLLRAIENRTEL"
     gene            17422..18468
                     /locus_tag="VCA20_000632"
     CDS             17422..18468
                     /locus_tag="VCA20_000632"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEP1834487.1"
                     /translation="MSKETLILLYGGRSAEREVSVLSAESVMRAINYDKFFVKTYFIS
                     QTGDFIKTQEFSQTPSADEKLMTNATIVEEQKIRPSDIYEEKAVVFPVLHGPMGEDGS
                     IQGFLEVLRMPYVGTNILSSSVAMDKITTKRVLESAGISQVPYVAVIEGENIDDKIAE
                     IEGKLTYPVFVKPANMGSSVGISKAEDLAGLRKALDLAFKYDSRVLVEQGVNAREIEV
                     GLLGNADVKTTLPGEVVKDVAFYDYDAKYIDNKITMEIPAKIDESIMDIMRDNAAKAF
                     RAFGGCGLSRCDFFLTEDGDIFLNELNTMPGFTQWSMYPLLWDNMGLAYPDLIEELVR
                     LAKEMFEKRESHLI"
     gene            18556..18936
                     /locus_tag="VCA20_000633"
     CDS             18556..18936
                     /locus_tag="VCA20_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEP1834488.1"
                     /translation="MYQTTVKGEGLFQALSQGYGEPVRTFGLTEKGETPVSLVNIGLA
                     ACITMCVQGYYASQEGNKTMPVQVESRLDNQRFEVLIGIGESVSAQKQKEILAYVEKK
                     CKVTALLKEDLQFETSFFVLESVE"
     gene            18937..20013
                     /locus_tag="VCA20_000634"
     CDS             18937..20013
                     /locus_tag="VCA20_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP1834489.1"
                     /translation="MFLAIEEMRQNKLRYGLILGLLVLISYLVFFLTGLAYGLMQENR
                     TAVDKWQADYVLLNSESNRLITASKIDTDLLDQVDASNKALIRQQAGVAYVDENATSD
                     EKEKVNIFAVESDSFIVPNIVEGRLYEKTGEVLVDKTLSEVEGFGLGDQIYLSGSDEK
                     VTIVGYTDNAYFGVTPVIYMDFTAFAELMQADQQQAATSNLASAIVVRGDVASLPNEL
                     EKVAIADFIENLPGYKAQNMTFGFMIGFLIVISAIVIGIFIFVLTTQKSPIFGLMKIQ
                     GLSNGYISGSVLAQTFLLAGVGTVLGLAGTYLSSLVLPSAVPFENNWTFYIVIGLALV
                     VFALLGASFSVRSIFKVDPLRNLS"
     gene            20023..20691
                     /locus_tag="VCA20_000635"
     CDS             20023..20691
                     /locus_tag="VCA20_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1834490.1"
                     /translation="MKTLIFENISKTFQDGSQTITALKPTNFSIEEGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPTTGRVLINQSDYSDLPEKKRAKLRYKDIGFVLQASNLIPFLTV
                     EKQLTLVDKVNKKAEPSKRNELLSELGVDHLKNKFPKDLSGGERQRVAIARALYNDPA
                     LILADEPTASLDSNRAFEVVELLAKEAKERNKSIIMVTHDQRMIEKSDKVYEMKDGVL
                     TQVR"
     gene            20746..22107
                     /locus_tag="VCA20_000636"
     CDS             20746..22107
                     /locus_tag="VCA20_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEP1834491.1"
                     /translation="MKLTLHEVAQVLGAKNDISLYPDTALNKLEFDSRLITEGDLFVP
                     LKGARDGHDFIPVAFENGCAVTLSEVSLDVPYILVEDCLAALQQLAAYYLEKTGVEVI
                     AVTGSNGKTTTKDMIHDVLATTYKTYKTQGNYNNEIGLPYTVLHMPDDTEKLVLEMGQ
                     DHLGDIHLLSEIAKPSLSVITLFGEAHLEFFGSREEIAKGKLQIADGMAAGNKLLLPA
                     DPIADKLLPSHVDLIRFGEQGDLQVTSLVESKDSLTFTVNFMDGDIFLPVTGKYNATN
                     AMVASYVGKTLGVPDEAIKSALAGLNLTRNRTEWKKAANGADILSDVYNANPTAMRLI
                     LETFSSIPANEGGKKIAVLADMKELGSQSVDLHNQMILSLLPDVLDTVIFYGQDIEGL
                     AQLASQMFPIGKVYFFRKNAEQDQFEDLVKQVRESLGKEDQILFKGSNSMNLGKVVEA
                     IEK"
     gene            22198..22881
                     /locus_tag="VCA20_000637"
     CDS             22198..22881
                     /locus_tag="VCA20_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HEP1834492.1"
                     /translation="MSDFFSNQISEAPNLDVLEHLFLMIIWFFMLYLCFRCYQKRWFH
                     IVFWWLQFIQIVSLYTWYIVADFPLSESLPFYHCRLAMLFILWAKPGPLKRYFAYLGL
                     FGSLSAFIHPVFDPFAFPHLTFFTFVIGHYALTVNCMLYLLSDLEGEMLKGKEVVKYT
                     LIMNMLILGVALLTGGNYGFLRQAPLVNTNNLPLNFFLVTCLLCFSILSIQAMLIAYL
                     KKESKQMDS"
BASE COUNT         6805 a         4172 c         5305 g         6674 t
ORIGIN      
        1 tttagtattg gttttaattg atacaaaatt cgtgtagaat acagtttaaa atatgaaaaa
       61 atgtaaaaaa ggtctataat ggtattatga aagtactatt gtatttagaa ggtaaaactg
      121 ttttagagaa atcaggaatt ggccgggcac ttcaacacca gatggaagcg ctagatatgg
      181 caggcattcc ttatacaaca gacctgctgg gagattatga tgtcattcat atcaatacct
      241 atggtccacg ctcttggtta ttgctacatg cagccaaacg gagtggaaaa aaggtaatta
      301 tgcatggaca ttccacgaag gaagattttc aaaattcctt tatcggttcg aataccctag
      361 ctccttttgt agggaaatat ctagcaagca tgtaccaaaa ggcagacttt gtcatcactc
      421 cttcagagta ttcaaaaaaa ctcatccagt cttacggtgt gacaacgcca attatagccg
      481 tttcgaatgg tattgattta gataaatata agaaggatga acgtaaggaa gcaatctttc
      541 gagagcattt taaaattgaa gaaggtcagc cagttgttgt ctgtgcagga ctttacttcc
      601 gacgtaaagg aattgaagac tttgtcaagg tagccgaaaa gatgccgcat gtccgcttca
      661 tttggctcgg ttcaatcaat aagtggctga ttccgtcata tattagaaaa attgttgagg
      721 gagatcatcc agcgaatgtc gagtttccag gttattttaa aggtgctgtt tttcaaggtg
      781 caatgagtgg agcggatgcc tttttcttcc catcgtatga ggaaacagaa gggattgttg
      841 ttctggaagc tcttgctagt catcagcatg ttgttctaag agatattcca gtttacgatg
      901 gttggattga cgaaaacgtt gccgaattgg gacatacagt ggatgatttt gtcgattcta
      961 ttcagaaaat cttagacaag caggttgata aacgtgaggc gggctaccag gttgcggtta
     1021 gtcgctcgat tgacaatgtt tcccaccaat tggtggatgc ctatcgacaa gtattggaga
     1081 tgtaaatgcg gattggattg tttacggata cctattttcc acaggtttca ggagtcgcga
     1141 cttcgattcg gactcttaag acagagttgg agaaattggg acatacggta tttattttta
     1201 cgacgacaga tgaggatgtg aatcgctacg aagattggga cattatccga attcctagcg
     1261 tacctttttt tgcctttaaa gatcgtcgag tggcctacgc aggttttaca gataccttaa
     1321 aaattgcaag tcgctataaa ctagatatta ttcatactca tacagaattt tctttgggga
     1381 ttttggggaa aatgattgcc aaggaactag atattccggt ggtacatact tatcacaccc
     1441 agtatgaaga ctacgtacac tatattgcca aggggaggat tattcgtccc gggatggtta
     1501 aatatttagt acgggcattt ttaaaagatt tggatggggt aatttgtcct agtgagatcg
     1561 ttgaagatct tttaatgagt tacaatgttc cgatttctaa acgagtaatt ccgacaggaa
     1621 ttgacttggt aaagtttgat agaccagaaa ttggcaagga tgaaacagat gagttgcggg
     1681 aacagctagg aattgctagt gatgaaacga tgttgctaag tctatctagg atctctcacg
     1741 agaagaatat ccaagcaatc gttcaggcaa tgccaaaaat tttgctggaa aatccaaagg
     1801 tgaagttggt cattgtcgga ggtggacctt atacagatga tcttcaggaa atgatagcgg
     1861 agcttaatct agatgaacac gtccagttga cgggcatgat tgcaccaagt gatacagcac
     1921 tttattataa agctgcggat ttctttattt ctgcttcgac cagtgaaacg cagggactaa
     1981 ctttcttaga aagcttagct tctggaactc ctattttggc acatagcaat ccctacttaa
     2041 aaaacgttat aacagataag atgtttggga cactcttcat tcgagaacag gagttggcag
     2101 atgccgtcgt tgaggcgatt gttgctacac caccaatgaa tccgtatgtg cttgataaga
     2161 agctctatga gatttcttct acgaattttg ggcgcaaggt ttttgaatat tatctagatt
     2221 tgaagatttc gtatgatttt agaaaagaac atactaatca agatagcgta gcagaagcat
     2281 tgataaaaga ggctatttac ctaccgcaaa aagtagtagt taaatcaaca gacacaacag
     2341 caaaaatatt gaaaaaatca gtcgaacaag taaaatcaat ccggaatttc tttgaataat
     2401 ttggaggttt caaatggaaa gattacaaat ggaaagttgg aagagtctgg ttttggcaac
     2461 tctcattgtt ctatccatgt atgcaggtat ttatctgatt gcctttcatc tgattagtga
     2521 caccatgctt tctaaggaac tggtggtcgg gaatatattg ctctatactc ccattgtact
     2581 attcctaaca ggtcttcttt atggattttt tcacggtttt tctcctaccc ttcttgcctt
     2641 gattttactg ctttatcctg taaccatctt tttctttaag gaatggatta ttctctatca
     2701 gattgtctac accttatgtg ctctgattgg aaatggggta ggagctctgc tattttctct
     2761 aagaaagaaa atgaggaaaa atccttgaaa aatcaggaaa tcctgttata atagctttta
     2821 gagacttatc aattctagga aatctttgag agagtgtgcg gtgggtgcaa gcacatagag
     2881 gataggattg acactactcg gtatgatttt atgcaaacat aaaagggtgg ttcccttatc
     2941 gccaatctga ggtagtcagg ctttttagtg tgactgcgaa tgaaggtgga accacgttac
     3001 gacgtccttt tgtgagggcg tcgtattttc ttttggagga ggaatgatgc tacctgatga
     3061 cttattaaga cggtgtcgag atgtgcgtcg tgcttacaga gatttggaac ttaagcacca
     3121 cgacaaagag tggtccattg aggagtgaaa cagtctgggg atagactgtt gcagctcaac
     3181 aacttaaaat aaagacttgt tgaccaatga tattggcaac atgaaccgtc tgattatgac
     3241 caagcagggt cgttactatg atgaaacgcc ttatactttg gagcataaaa tggctgaaaa
     3301 tatctggtgg ttgattgaac tggctgaccg cctggatatt gatattcaaa aagaaatgga
     3361 aaccttttta gctcaaaaag aagagttatt ggggattaaa aaataaaaat agaaaggact
     3421 agggtctccg tatttggttg aactgaatac gggctacgga ctgggccaaa aagataaacg
     3481 agaactagac gcttgcgtcg tcgttctgtt tcctattttg gctgtgtccg cttaacgccc
     3541 tttatatctt atattatgct taaaattact ttcccagatg gtgctgttcg tgagtaccag
     3601 gctggtgtga cgacttttga aattgcagag agtatcagca agtctttggc gaaaaaggct
     3661 cttgctggta agttcaatgg taaattgatt gacaccactc gtgctattga agaggacggc
     3721 acgcttgaaa tcgtaacgcc tgaccacgag gatgccttgg acatcttgcg tcactcagcg
     3781 gcgcacttgt ttgcccaagc ggctcgtcgt cttttcccag acatcaagtt gggggttggt
     3841 ccagctattc aggacggttt ttattacgat acggacaatg cggctggtca gatttcaaat
     3901 gaagacctgc cacgcattca ggaagaaatg atgaagattg tcaaggaaaa cttcccttca
     3961 gaacgccgtg aggtgaccaa ggaagaagct cttgagattt tcaagaatga tccatataag
     4021 ctggaattga ttcacgagca ctctgacgac gagggtggtt tgaccatcta tacccagggt
     4081 gaatacgttg acctctgtcg tggtccacac gttccgtcaa caggtcgtat ccagattttt
     4141 gaattggtca atgtagccgg tgcttactgg cgtggtaaga gcgaaaatcc aatgatgcaa
     4201 cgtgtttatg gtacagcttg gtttgacaag aaagacctca aggcttacct tcaaatgcgt
     4261 gaggaagcca aagagcgtga ccaccgtaaa cttggtaagg aattggatct tttcatgatc
     4321 agccaagaag ttggtcaagg tttgccattc tggttgccaa atggggcgac tattcgtcgt
     4381 accttggagc gttacatcac agacaaggaa ttggcttctg gttaccagca cgtttatacg
     4441 ccaccattgg cttctgttga actctataag acttcgggtc actgggagca ttactcagaa
     4501 gatatgttcc caactatgga catgggcgac ggtgaggagt ttgtgcttcg tccgatgaac
     4561 tgtccgcacc acattcaagt ttacaaaaac catgtgcgtt cttaccgtga attgccagta
     4621 cgtatcgctg agcttggtat gatgcaccgc tatgagaaat ctggtgctct tactggtttg
     4681 cagcgtgtac gtgagatgac gcttaatgac ggtcatattt tcgtgacacc tgagcaaatt
     4741 caagaagagt tcaaaaaagc tctccagttg attatcgatg tgtacgcaga tttcaaccta
     4801 aacgactacc gtttccgcct gtcttatcgt gacccagagg ataaagaaaa gtactatgac
     4861 aacgatgaga tgtgggaaaa tgcccaacgc atgttgaaag gtgccatgga tgaaatgggc
     4921 gtggactact ttgaggctga gggtgaagct gccttctacg gtcctaaatt ggatattcaa
     4981 gtgaaaactg cccttggtaa cgaagaaacc ttgtcaacta ttcagttgga cttcctcttg
     5041 ccagaacgct ttggtctgac ctatattggt gctgacggtg aagagcaccg cccagtcatg
     5101 attcaccgtg gtgttatctc gactatggaa cgcttcacag ccatcttgat tgaaacctac
     5161 aagggtgcct tcccaacttg gttggctcca acgcaagtga ccatgattcc aatctctgtg
     5221 gaagctcatc tggattacgc ttggaaggtt gccaaggaat tgcaagaccg tggcgttcgc
     5281 gtacatgtgg atgagcggaa cgagaagatg caatacaaga tccgtcagag tcagaccagc
     5341 aagattccat accaactcat cgtcggtgac aaggaaatgg aagacaatgc tgtcaacgtc
     5401 cgtcgctacg gaagcaaggc aacacaaact cagtctgtat cagaatttgt ggaccatatc
     5461 ctagcagata ttgctcgcaa gtcacgtcca gctgaaactg aataataatt ataaggagga
     5521 gtccgaaagg gcttcttttt gacagcaaaa actcccaagc gattggctgg gagtgctagt
     5581 atcacttatt agttactttt cttttgaggg taggaaatca ccttcccttg aaatacttta
     5641 tccagtatag gaattctttt gacaatcatc aagactaggg aattgatgat aagaagtaat
     5701 ccaaaactta tgaggaggag tacaggtgta ggaaaatcta aaaaatattt cttaatgaaa
     5761 agagtgagaa tagttaggat ataggtatgg attagataaa agtatagtga gcattggcca
     5821 aaccaggata tgactgcagg caatcttctc tctaaagtga ttatgataga aacgatcagg
     5881 taggtcatag ctagtccgtt tgcatatcgt aggaaggtgt gaaatggcag gtgctgactt
     5941 gggtaggttt ggtaggactg taaatagatg agatagaaga tggtgaccag accggctaaa
     6001 ctgtgaaaaa cggtctgtct aaccattttc ttgtgaattt ctttcttggc aaaataaata
     6061 cctaaaaaat aaaatagcgg taaagaagtc aggtgttgta acccgattgg taagttgcta
     6121 atatttaagg gaccacccca taggacagca aatagtgtaa aaagaccaat gcaaatcagt
     6181 gaaactggtt ttgttagttg caatcgatgg agaataagag ttgaggtggc cataaggatt
     6241 aaaaaccaaa tataccacat agatgtatct ggcaggagaa aattagaaac gaggtgacta
     6301 ggttgtattt tatcaacagt gtaacttgaa aagaggattt tgctaattac aatactactg
     6361 ccaaagactg cgtatatttg caacagtcga aatatatata tatatatata agtattattt
     6421 ctaggttttg gttttaggta aaaatgatag aaactaaagc cactgacaaa tacaaaaaat
     6481 ggcataagga aagagtggag ccaggcttct agtcgccaaa gagggatatc ggtatatgga
     6541 agagcgtttc ggatataacc gactgtatgc agaaataaaa cagctaaaat tccaaaacct
     6601 ttcagctggt caatatagat gagtcgtttc ataagtttct cctttattta actctaaact
     6661 atttgggaac agaggcattt ccaaaatggt ttgagtacat tttctttgat tatatcataa
     6721 aaccagcttt ctcgagaaaa ctggttcaga ttgtttattc aatactgctc gcccaagact
     6781 tagccacata acgtgacagg cttgaaatag caaagttgat gctaaagtaa atcaaggcta
     6841 ccatgagata gagggcaaaa acttgttctg cctcaaagta gcgtcccatg aggatttgac
     6901 tcgaaccgaa gagttcttgc agggcgataa cggagtagag gaaactagtg tccttgatga
     6961 ccgttacaat ctgcgagatg atggctggca gcatcttgcg aatggcctgc ggcatgatga
     7021 tatagaccat gatttgcact gaggtaaaac cctgtgacaa gccagcttcg gtctggcccg
     7081 gtccgatggc gttgagacca ccacggataa tctcggccat ggctgccgtg gtaaaaatag
     7141 taaaagcagt aataccagca ggcgtggact tcatttggaa gactaagaat actgtgaaaa
     7201 tccaaagcaa gtttggcacg ttacgaacaa actcgatata gatagttgca gtccacttga
     7261 aaattggatt ttttccattt cgcatgatgg cgagaatcat tccgaaaaga atggacagga
     7321 ttaccgaaat gcttgagata taaagtgtta acttcaaccc actccacaag agttggaagg
     7381 ttgaaggttt gagtaattct aaaacataat tcatcgttca acctcctttt agtgtgaaaa
     7441 ggctgactta ttcttttctt caatgcgacg tccccagctt gcaacaggga agcacatgat
     7501 gaagtaaagg aaagcagcac cagcaaaggc tgggatgtag ttggcattca tagcagacca
     7561 agattttgtc ataaacataa tatcagctcc agagatgatg gcaacagtag ccgtattctt
     7621 aattaagttt actacttggt tggtcaaagg tggaagaatg gtcggaaggg cctgtggtaa
     7681 aatgatgatt cgcatggttt cagactgagt aaatccttgt gaaagtgcgg cttcagtctg
     7741 cccagtaggc acagcctcaa tccctgcacg aatgacctct gcgatatagg ccccgtgata
     7801 gagtccgacg cagataacag ctgtccagta gatggacggc atgagaacat agtttgaaat
     7861 cagtggaagt ccataataaa caatcatgaa ctgaaccaaa agtggcgtgt tctgatagta
     7921 ttcaacatag acacgtgcga tgatttttaa aactttattt ttacttgaag agagactacc
     7981 aaagacaatt cctaaaaaca tggccatgat aaaggcaccg acagaaatgg ctaaggtaaa
     8041 gagaaaacct tggcaaaagg ttggaaaatc cttgaaataa ctgacccaat tctcccaagc
     8101 gtaaggacta gttgttaaaa ctgtcatatt gtttcacctt tctattcgtc agtttctgtg
     8161 actggtttga gaccattggc atcatagatg ctttgtaggc taccatcggc agtccatttt
     8221 tctacgagac tatttaagta gtcgttaagg tctttgttag ataatttagt aacaattcca
     8281 tagtcagaag ctgagaagct aaagtccatg agttcagatt tcgaactgat atacccagaa
     8341 aggatagatt ggtctactga aaatgcgtct gtacggtgag cacgtagcga gacagagagt
     8401 tctggataag aaccaagctc tgcaaaattg acagagatac catatttgtc tgctagttca
     8461 gatatgaggg tacgtgtaat ggaaccctga gctacaccga ttgtctttcc atccaggtcg
     8521 gtaaaggtct taattccact atccttattg accaagaagc caacggcatc agtgtagtaa
     8581 ggagttgtga aattgtagag caacttacgt tcctcagtga tggtaaaagt tgcgatgacc
     8641 atatccactt gaccgttatc taagagtggt ccgcgagttt gtgccgtaac aggggtaaat
     8701 tcaatctcca cccctaactc gtctgcaatt ttgcgagcaa tatcaatctc cattccctcg
     8761 aattcaccag tatcagggtt cttgtagcca aagtttggga cgtcctgttt cactccgacg
     8821 cggagaacgc ctctatcgat gatagcttga acttgatcac tattggtaat ggatgcgggt
     8881 gtttctgttt gagctactgg ttgttttgcg aaaacgagaa gaataagtcc aaaggccaat
     8941 aatataattg caaagaaaat gttgattttt ttcttatcca tagacttcct cctagagttt
     9001 tactttgtca gattcgtggt tgataatctt gctgaggaat tgttgggcac gaggctctgt
     9061 cggattgtca aagaagccat tcacatctgt tgtatcaact aaaacttctc cctcagccat
     9121 gaagatgatg cgatcagcta cttcacgggc aaagcccatt tcgtgtgtta cgacaatcat
     9181 gttcatcccg tcacgtgcca atttttgcat aactgcaagt acatcgccga tagtttctgg
     9241 gtccaaggca gatgttggtt catcgaagag tagaagctca ggtttcatag ctaagccgcg
     9301 agcgatggca acacgttgtt tttggcctcc ggatagcata gcagggtagg aatccttccg
     9361 atcccagagg ttaacaaatt caagatactt ttgagcggtt ttttcagctg tagccttatt
     9421 gattcccaaa accttaatcg gtgccaaggt aacattttct aacacggttt tgtgtggata
     9481 aaggttgaaa tgctggaaaa ccatgccaac ttctttgcga agtgatacga gctctttgat
     9541 agaagtagag gaaatatcat gtccattgac gatgagcgtt ccttcatcaa ttgcctctaa
     9601 accgttaatc gtgcgaatga gagtagattt tccggatccg gaaggtccaa ggagaacgac
     9661 aacctgtcca ggttcgaatt ccaagttgat attgcgtagg gcgtggtagt cgccatagta
     9721 tttgttgaca tctttaaatt ctactaaagg cataaaattc tccttgcata gtgtttttta
     9781 ggtattacca tcttagcatt gcctatatac aatgtcaagt aaattctgac aatttaatgt
     9841 attgtatgta acaaaatgta acatcgttgt gtcagatggt tttgaaatat gaatttttta
     9901 ggtaagtttg atataataaa agaaataaag caaaggtaag atactaatga aaaaaatatt
     9961 aattgtagat gatgaaaaac caatctcaga tattattaag tttaatatga cgcgtgaggg
    10021 atatgaagtt gtgacagctt tcgatggacg tgaagccttg gaagtatttg aggctgagtt
    10081 tcctgacatt gtcattttgg acttgatgct accagaattg gacggactag aggtcgctcg
    10141 aacgattcgt aagaccagca atgttcccat cttaatgcta tctgctaaag atagcgaatt
    10201 tgataaggtt atcgggcttg aaatcggggc ggatgattat gtgaccaagc ccttctctaa
    10261 tcgcgaattg caggcgcgtg ttaaggctct tcttcgtcgt agtgaattag cagagacgca
    10321 gacaaatctt gagtcaacag gaactccaga gttggtgatt ggcgatttgg tcattctgcc
    10381 tgatgcgttt gtcgctaaga agcatggcaa agagctggag ctgacccatc gtgagtttga
    10441 attgctccac catctggcca aacacttagg tcaggttatg actcgagaac atctattgga
    10501 aacagtttgg ggttatgatt actttggtga tgtccgcacg gtggatgtaa cgattcgtcg
    10561 tctgcgtgag aaaattgaag atacaccaag cagaccagaa tacattctta ctcgtcgcgg
    10621 agtgggatat tttataaaag gaaatgatta atcaattacg ttatttaatg accacggcag
    10681 aattttggtt tgttgtcatt ttgattggct ttctaattgc tctgacggtc cttttgattg
    10741 aaaactatcg ggataataag caaattaaac aactcaatca aaaagtcaat gccttaattg
    10801 aggggaatta tgcagatgtg ttagacatga ggggaagtcc tgaaatcaca gatatggcga
    10861 attctcttaa tgatctgtcc gaggtcatcc gcttaactca tgataattta gaacaagaaa
    10921 agacgcgtct gacttccatc ctgtcctata tgagtgatgg tgttattgct acagaccgta
    10981 tcggacggat catcatgatt aacgatatgg cccagaaaca gttggggtta tctaacccaa
    11041 agcaggagca gtatcacctg ctcgaggttt tagatttatc agaccgttat acattgagag
    11101 atttgttggc tcagacacct gagattgtga ttgatcacac caatgaaaac gaagaatttt
    11161 tgactttacg cgctaatttt gccacgattc gtagtgagag tggtctgatt tctggtttgg
    11221 tcgtcgtctt gcatgatatg accgagcagg ccaaggaaga acgggaacgt aggctgtttg
    11281 tgtcaaatgt aagtcatgaa ttgcgtacgc cattgacttc cgttaaatcc tatctagagg
    11341 ctctggatga gggagcgctg acggagtctg ttgcaccgag ttttgttaag gtatccttag
    11401 atgagaccaa ccgcatgatg cggatgatta cagacctcct aagcttatcg cgtattgata
    11461 atcaagttgg tcagatagat gtcgaactga taaactttac tgcttttgtg acctttattc
    11521 ttaaccgttt tgaccaaatg aaaaatgctg attctgataa ggtctatacg attgttcgtg
    11581 actatcaaat cagtcctatt tgggttgaga ttgatacaga taagatgact caggttttgg
    11641 ataatatctt aaataatgcc attaaatatt cgccagacgg cgggacaatt accttcagca
    11701 tgaagaccac agatagccag ttgattgtat ccgtctcaga cgaaggtctg gggattccga
    11761 aagcagattt acctagaatt tttgatcgat tctatcgcgt ggacaaggcg cgatctcgtg
    11821 cccaaggtgg tacaggtctt ggtttagcta ttgcaaaaga aatcgtaaaa caacataaag
    11881 gctttatctg ggctaaaagc gaatatggtc atggctcaac gtttacaatt gtcttgccgt
    11941 acagcaagga catcgcacta gatgagtggg atgattcaga tgaggaagaa taggagaata
    12001 tgatcggaaa aggttttaat tatagtattt tagcttcggg gtccagcggg aattgttttt
    12061 acctagaaac agataaaaag aaaatcctag tggatgctgg cttatcaggg aaaaagatta
    12121 ccagtctttt ggcggaaatt gatcgaaagc ctgaggatat tgatgctatc ctggtaacgc
    12181 atgaacatag cgatcacatc catggtatcg gtgttttagc acgtaagtat ggcatggaca
    12241 tttatgccaa tgaattgacc tggcaggcta tggagagcaa attgggcaag attgatgtgg
    12301 ctcaaaaaca tatcttcgaa ttgggtgcta tgaagacttt tggcgaccta gatattgagt
    12361 catttggagt tagccatgat gctgcatgcc cgcaatttta tcgttttatg aaggatgaca
    12421 aatcctttgt tatgttgacg gatacaggct atgtcagtga ccgcatggtt ggaattgtag
    12481 aaaatgccga tgcttatttg attgaatcga accatgatat tgaaattttg cgctcgggtt
    12541 cttattcatg gaatttgaag caacggattc tatccgataa aggccatctt tgtaacgaag
    12601 atggagctga tgccatgatt cgctcgttgg gaaatcggac caaaaagatt tacctagggc
    12661 atttgtcaaa ggaaaacaat atcaaggaat tggctcacat gaccatggtt aatcagttgg
    12721 cgcaggctga tttaggagtt ggagtggatt ttcaagtcta tgacacatcg ccagatacag
    12781 cgactccctt gaccaagatt tgacaaattc attgaacttg tttagaataa atatataaaa
    12841 tacgtgaggt gaagttacat gaataattaa gctacttatt agtcgttctt aaaacttgtt
    12901 gaaaatcaat tatttgataa tttagtcttt tagtttgctt ttagtactag gcaacgagct
    12961 gcaggctgta ctgaagtacg gcaaagcgag ttaacgtagt aataaaagat aaactaaatg
    13021 actacacaag ggataagact gctcataagg gcttactatt catgtgactt tttccatagg
    13081 gaaaggtcat gttttgcatg gcttttttct tgttcattga gtagaatagg ggcccttttt
    13141 gtgaagaaaa gaggtctata tggaagtaat aaaatgtatt cgattagaga aggaatttgc
    13201 gggacgtagt ttgtttacaa tccagcagtt gagccttcaa gctggtcaaa aggttggttt
    13261 ggttggaaat aatggtgttg gtaagtctac ttttttgaaa atcttactgg gactggatag
    13321 ggattttgct ggtcagattg aggtaaaagc agactgggcc tatgtacccc agttacagga
    13381 aaggagctcg ctttcaggtg gggaacaggt ttggaagtct attcaagagg cttttgccca
    13441 aagaccacag ttgttaatca tggatgaacc gactgccaat ctggatcaag aacaccagga
    13501 aaagctaatc aaacaaatca aacgctatcg cgggagtcta ctagttgtta gtcatgatcg
    13561 gcattttctc aatcaaatag ccagtcatat ttggcattta gaagaaggaa gtattcaggt
    13621 ctacacaggg aattacgagg cgtttgtaga aagtcgccgg gctaggcgag aaggacagca
    13681 gcaggcctat gaagcctatc agaaaaaagt cgctcaacta aaaaaagccc aacatgaacg
    13741 tcaagcaaag gctcagaaga tgaacaagaa aaagaaaggc atttcttcgt cagaatggaa
    13801 ggtcaatgct atgatgggtt cctatgatag tcaagccaag tcaatagcta agtctgctaa
    13861 acagttagag aaaaggattg agaggttgga aaaggttagt cagcccagaa aggaggctca
    13921 tattaaaatg gaatcaaaag gggccttaga tactgattta cacagactat ttcttttgca
    13981 ggctggtcaa gttttgatgg atgaagtgta tctatttgat ttccctcaga tagggatgac
    14041 gtttggagag aagttagcct tagtgggggc gaatggttct ggaaagacta gttttgttcg
    14101 gaaattgatt tcaaaagaat tagaaggcta ttacaatcca aaattaaaaa ttgcttattt
    14161 tgcacaggat ttgataaacc ttgatgaaga aaagactgct ttggaaaatg ttagttgtac
    14221 ctctcttcaa gataggatga cgattctcaa tttattaggt atgttaggac tttcttacga
    14281 taaggctcag cagaaggtag caaatttgtc tggtggtgag cgtgttcgtc tctccttagc
    14341 taaggtattg ttggcggatg caaatctttt gatattagat gaaccgacaa attttctaga
    14401 tatggcagct attgaagctt tggaaacatt cttgaaagaa tatgaaggaa gtgtcctctt
    14461 ggtttcccat gatcaacagt ttgtggaaac atctgttcac agtcaatggg agatagtgca
    14521 agcttgtctc gtgaagaaat cataagaaaa tattttctga aaaggaggct ctgtctaaaa
    14581 aatatttttt gcctgtggta taataagaag gatatttcta gaatgaggtc tttgaagtga
    14641 aaaaaagaaa gaacaagcct ttggaacacg atggaaattt tatttccctt cgtttaaata
    14701 ttttattttc tatcgtgatt tttctttttc tagttttgat attgcgtttg gcagatatgc
    14761 agattatcaa tcatgatttt tatagtaata agctgtctac tgccagtcag aaaatcatca
    14821 gcaatggctc tattcgtggg cagatttacg atgcgcaggg aaaacctttg gttgaaaacg
    14881 agattcagca ggtcgtctcc ttcacacgtc ccaacaagat gtctgctcag gaaatgaaag
    14941 aagtagctaa caaactcttg cagtgggtca atgtgtcgga cgtaacaatt actcaacgtg
    15001 acaaggctga ctattattta gcggatgaag aagtttatcg tcaggtagtc gaggccttgc
    15061 cagaagataa aaaatacgat tctgatggca actacctgag tgaatcgaca atttattcta
    15121 atgcggttga tagtatccct gctgaagcat tgcaatattc tgagcaagac agcaaggcaa
    15181 ttgagctatt taagcagatg aatggagcta cttacttctc tactgttaat ttggtaacag
    15241 atactctaac tgctgaacag gttgctcata tcgtagccaa tgaaagtcaa cttccaggca
    15301 tttcaacgac taacaactgg caacggacga ttttaccaac ttccctcagc tctattattg
    15361 gtacagtaac gagtgaacag gctggtttgc ctgcggaaga tgcggaatac tatctctcca
    15421 agggctactc atcaaatgac cgtgtgggaa ctgcctacct cgagaaacaa tatgaagaag
    15481 tcttgcaagg tcaacgtgag aaaaaacaaa tcaacctaga tcgaaatggc aatgtcgaaa
    15541 gtattgagac aatccaagaa ggacagcagg gaaataatat taaactgact attgatttag
    15601 cctttcaaga tggggtcaat gctattttga aaaaatactt tgaaagtgag ctggctactg
    15661 gtagtgcact ttactcggaa ggagtttatg cagtcgcatt ggaaccttca acaggtgcgg
    15721 tgcttgccat gtctggatac agtcatgaaa aaggagccgg tagcataaca gaagatgccc
    15781 ttggtactat tacaagtgtt ttcacaccag gttctatcgt caaaggagcg acaatcagct
    15841 ctggttggga aaatggagtt atcagtggca atcaagtgca gttagacgaa ccaatctatt
    15901 ttgcaggttc agcaccaatt acatcttggt atggggccta tggtagtttt aatattgatg
    15961 cgacagaggc tctagaatat tcttctaacg tctacatggt taagattgct ttgggactac
    16021 ttggtcagac ctactcagct aacatgtatt tgaatgatgg cgatacattg acaaatgcca
    16081 tgactaagct tcgttcgact tttgcagagt atggtttagg tgcttcaaca ggtattgacc
    16141 ttccactcga atctacagga tttttaccgg aagagtattc gacagccaat tttattacca
    16201 atgcttttgg acagtttgat aactatacac cgatgcagat ggcacagtat gcagcgacca
    16261 ttgcgaataa cgggacacgg atttctccac acctcgttga gggaatttat gggaataatg
    16321 cccaaggtgg tttgggggaa ttaattgaaa ccgtatctgg taaggagatg aatcaggtta
    16381 atatttccgc tgaggatatg tctcttcttc gtcaaggttt ctaccaagtt gtcaatggta
    16441 gtgggcgctt taatacaggt agtgctatcg gtcgtggtgc ggctgtgacc atcagtgcca
    16501 agactggtac agccgaaacc tacacaacga cagcttctgg tgatgtggtt actgcggtca
    16561 ataccaacgt tgttgcttat gcgcctagtg ataatcctca aattgcagta gcagttgtcc
    16621 taccaaattt gaccaatcaa aattcaacaa ctactaagac aattactcaa gaaattatca
    16681 atctatatca atcacttcac ccaatgaatt aaggaggctt atgctttacc ctacacctat
    16741 tgccaagtta attgatagct attctaaatt gccaggtatc ggtataaaaa cggctacccg
    16801 tttagctttt tataccattg gcatggagga tgatgtcgtt aatgagtttg caaaaaattt
    16861 attggcagcc aagcgagacc tatcttattg ctctatttgt ggtaatttga cagatcagga
    16921 cccctgtgcc atttgccagg actcaacgcg agaccaatct actattttga ttgtagagga
    16981 tagtcgagat gttacagcct tggaaaatat tcaagaatac catggtcttt atcatgtctt
    17041 gcatggcttg atttctccca tgaatggtat cggaccagat gatattaatt tgaaaaccct
    17101 cctcactcgc ctgatggaca atgaggttac agaagttatt gtggcgacca atgcaacagc
    17161 agatggagag gctacatcta tgtatatctc acgtgtcctc aaacctgcgg gaatcaaagt
    17221 gaccagacta gctcgtggct tagcagtagg cagtgatatt gaatatgcag atgaagtgac
    17281 cctgttgagg gctattgaaa accgcacaga gctatagcag ttctgtagtt agatacgttc
    17341 ctaattttaa gataggatag atatgatttg ttaaattatg ctatactaag agtaatagcg
    17401 ttgatacagg aaggaaaata tatgtcaaaa gaaaccttga ttttactata tggtgggcgt
    17461 tctgcagagc gtgaggtatc tgttctgtct gccgaaagcg tcatgcgtgc catcaattat
    17521 gataaatttt ttgtcaaaac ctatttcatt agccagacag gggactttat taagacgcaa
    17581 gagttttcgc aaacaccgtc tgccgatgaa aaactcatga caaatgcgac gattgttgaa
    17641 gagcaaaaaa tccgtccgag cgatatttat gaagaaaagg cagttgtttt ccctgttctt
    17701 catgggccaa tgggagaaga cggatctatt caaggcttct tggaagtgct tcgtatgcct
    17761 tatgttggca ctaatatctt gtcttcaagc gttgctatgg acaaaatcac taccaagcgc
    17821 gtacttgaat cagcaggaat ctcccaagta ccttatgtgg cagttatcga gggtgaaaat
    17881 atcgacgata agatcgcaga gattgaagga aaattgactt atccggtctt tgtaaaacca
    17941 gccaatatgg ggtcaagtgt cggtatctca aaagctgagg atttagctgg cttgcgtaag
    18001 gcacttgatc tggccttcaa gtatgatagc cgtgtcctcg ttgagcaggg agttaatgcg
    18061 cgtgagattg aggtcggtct tttgggaaat gctgacgtga aaaccaccct tcctggcgaa
    18121 gtagtcaaag acgttgcttt ttatgactac gatgccaaat acatcgacaa caagattact
    18181 atggaaatcc cagctaagat tgatgagtct atcatggata tcatgcgtga taatgctgcc
    18241 aaggccttcc gtgctttcgg tggctgtggt ctttctcgct gtgatttctt cctaacagaa
    18301 gatggcgaca ttttcttaaa tgagttgaat accatgccag gatttaccca gtggtctatg
    18361 tatccactcc tttgggataa tatgggcttg gcttacccag acttgattga agagcttgtt
    18421 cgtttggcta aggaaatgtt tgaaaaacgg gaaagtcacc tcatttaatt attatcaaga
    18481 ttggaatcat tccaatcttt tttcttgcaa aggtttgcaa aaaggaataa aatggaacaa
    18541 gttctggagg tgtttatgta tcaaacgaca gtaaaagggg aaggtctgtt tcaggctctt
    18601 tctcaaggtt atggcgaacc ggttcgtacc tttgggttaa cggagaaggg tgagactccg
    18661 gttagtctag ttaatattgg cttagctgcc tgtatcacca tgtgtgtgca gggctactat
    18721 gccagccaag aaggcaataa aaccatgcct gttcaggtgg agagtcgctt ggacaatcag
    18781 cgttttgagg tgttgattgg aattggtgag tcggtttcgg cgcaaaagca gaaagagatt
    18841 cttgcctatg ttgaaaagaa atgtaaagtc acagctcttt tgaaggaaga cttgcagttt
    18901 gagacaagtt tttttgtctt agaaagtgtg gagtagatgt ttttagcaat tgaagaaatg
    18961 cggcagaata agctgcgtta tggtttaata ttaggactac tggtcctgat ttcctatctg
    19021 gtctttttcc tgactggttt ggcttacgga ctcatgcagg aaaatagaac agcagttgat
    19081 aaatggcagg cagactatgt cctgcttaat tcagaaagca atcgcctgat tacagcatca
    19141 aaaattgata cagacttgct tgaccaggtg gacgctagta acaaagcctt gattcgccaa
    19201 caagctggag ttgcctatgt ggatgaaaat gccacctcag acgaaaaaga aaaggtaaac
    19261 atctttgctg ttgaatcaga tagttttatc gtcccaaata ttgtagaagg tcgtttgtat
    19321 gaaaaaacgg gagaagtcct tgtagacaaa accttgtcag aagtagaagg ttttgggcta
    19381 ggtgatcaaa tctatctatc aggttctgat gaaaaagtga ccattgtagg ttatacagac
    19441 aatgcttatt ttggagtgac gcctgttata tatatggact ttacggcatt tgcagaattg
    19501 atgcaggctg atcaacaaca agctgcaacg agtaatcttg ccagtgccat tgtcgttcgt
    19561 ggagatgtcg cttctctacc aaatgagctg gaaaaagttg ctattgcaga ctttattgaa
    19621 aatctgccag gatataaggc acaaaatatg acctttggct ttatgatagg attcttgatt
    19681 gtcatctcag ccattgttat tggtattttt atctttgttt tgacaacaca gaaatctccg
    19741 atttttggct tgatgaaaat tcaaggtctt tcaaatggct atatttcagg ttctgtattg
    19801 gctcaaacct tcctacttgc tggtgtggga acagtccttg gattggctgg gacttatttg
    19861 tcatcccttg tcttgccaag tgctgttcca tttgagaata actggacctt ctatattgta
    19921 atcggcctag cactggttgt atttgcccta ttgggtgcta gtttctctgt acgttctatc
    19981 tttaaggtag atccattacg aaatctatct taggaggttg ttatgaaaac acttattttt
    20041 gaaaatatta gtaagacctt ccaagatggc agccaaacca ttacggctct taaaccaaca
    20101 aattttagca ttgaagaagg cgaatttgtt gccataattg gaccatctgg ttctgggaag
    20161 tcaacatttt tgaccttggc aggtggttta caaacaccga caacgggtcg cgttttaatc
    20221 aatcaatcgg actattctga tttacctgag aaaaaacgtg ccaaattgcg ctataaggac
    20281 attggttttg tcttgcaggc ttccaatttg attccatttt taacagtcga aaaacaatta
    20341 accttggttg ataaggtcaa taagaaagct gaaccgagca agcgaaacga actcctatct
    20401 gaattgggtg tggaccatct caaaaacaag ttccctaagg atttgtcagg cggagaacgg
    20461 cagcgtgtag ctattgcccg tgcactttat aacgatccag ctttgattct agctgatgaa
    20521 ccaacagcca gtctagacag taatcgcgct tttgaagtgg ttgagttatt agctaaggaa
    20581 gccaaagaac gaaacaaatc catcatcatg gtaacacatg accagcgcat gattgaaaaa
    20641 agcgacaagg tatatgagat gaaagatggt gtcctcactc aagttcgata aaaaatggta
    20701 taatagaaag agcgaaagct cttttttagt ttattggaga aaacaatgaa attaacccta
    20761 cacgaagtcg ctcaggtctt gggcgcaaaa aatgatatta gtctttatcc tgatacggca
    20821 ctcaacaagt tggagttcga cagtcgcctg attacagaag gggatctttt tgttcccctc
    20881 aaaggtgcgc gtgacggtca tgactttatt cctgttgctt ttgaaaatgg ctgcgcagta
    20941 accctgtctg aagtcagcct tgatgttccg tacattctag tagaggactg cctggctgcg
    21001 cttcaacaat tagcggccta ctacctagag aaaacagggg tagaagtcat tgccgtgaca
    21061 ggatcaaacg gtaaaacgac caccaaggac atgattcacg atgttctggc gacgacttac
    21121 aagacttaca agacacaagg caactacaac aacgaaatcg gtctgccata caccgtcctc
    21181 cacatgcctg atgatacaga aaaactggtt ttggaaatgg gccaagacca cttgggtgac
    21241 attcatctcc tatctgaaat tgccaaacca agtctgtcgg ttattactct atttggcgaa
    21301 gcccatttgg aatttttcgg ctcacgagaa gagattgcca aaggaaaatt acagattgcg
    21361 gacggcatgg cagcgggaaa taaattgctg ctccctgcag atccgattgc ggataagctc
    21421 ttgccgagcc atgtagacct cattcgcttc ggtgagcagg gagatttgca ggtgactagt
    21481 ctggtcgagt caaaagatag cctgaccttc accgtcaact ttatggacgg cgacattttc
    21541 cttccagtaa cgggtaagta caatgccact aatgctatgg tagcaagcta cgtaggtaag
    21601 actttgggtg tgccagatga ggcaatcaag tcagccctag ctggtctgaa cttgacccgc
    21661 aatcgtaccg agtggaaaaa agcagcaaac ggagccgata ttctcagtga tgtctacaat
    21721 gccaacccaa cggccatgcg cttgattttg gaaaccttct ctagcattcc agccaatgaa
    21781 ggcggcaaga aaatagctgt tttggcagat atgaaagaac tggggagcca gtccgtcgat
    21841 ctccacaatc agatgatttt gagcctgttg cctgatgtgc tagacaccgt cattttctac
    21901 ggtcaggata ttgaaggtct ggcccagttg gctagtcaga tgttcccaat aggaaaagtc
    21961 tatttcttta ggaaaaatgc agagcaggac caatttgaag atctggttaa acaggttagg
    22021 gaaagtctag gaaaagagga ccaaatcctc tttaaaggct ctaattccat gaatcttggg
    22081 aaagtagtgg aagcaataga aaagtagaga ggcgagacct ctctttttta gcaagtcttg
    22141 tcaaaacatc gaaaagttga tatactaact tttggggaaa gagaggaatt taataagatg
    22201 agtgattttt ttagcaatca gatttctgaa gcgccgaacc tggacgtgtt ggaacatctg
    22261 tttctcatga ttatttggtt tttcatgctt tatctttgtt ttcgttgtta tcaaaaaaga
    22321 tggtttcata ttgttttttg gtggcttcaa tttatacaga ttgtctccct ctatacttgg
    22381 tatattgtgg ctgattttcc tctttctgag agtcttcctt tttatcattg tcgcttggct
    22441 atgctcttta ttctttgggc gaagcctgga cctttgaaac gttattttgc ctatctgggt
    22501 ctttttggat cgctttcagc ctttattcat ccggtatttg acccgtttgc ttttccacac
    22561 cttaccttct ttacttttgt tattggtcac tacgccttga cggttaattg tatgttgtac
    22621 ctgttatctg atttagaagg ggagatgttg aagggaaaag aggtagtgaa atatacgctt
    22681 attatgaata tgctgattct aggagtagct cttttaactg gcggaaatta tggatttttg
    22741 agacaggcac ctttagtgaa tactaataat cttcctttga atttcttctt ggtaacttgc
    22801 ctactctgtt tctctatttt gagtatacaa gctatgttaa ttgcctattt gaaaaaagaa
    22861 agcaaacaaa tggattcgta gatattgaaa aaataagact tgaaattagt ttttcaagtc
    22921 ttatttttag ttatgtgact tttagtccgt cacgcc
//
LOCUS       DATUCG010000020        22787 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.021, whole genome shotgun sequence.
ACCESSION   DATUCG010000020 DATUCG010000000
VERSION     DATUCG010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22787
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.021"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(1..1565)
                     /locus_tag="VCA20_000638"
                     /pseudo
     CDS             complement(1..1565)
                     /locus_tag="VCA20_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691454.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            complement(1562..1885)
                     /locus_tag="VCA20_000639"
     CDS             complement(1562..1885)
                     /locus_tag="VCA20_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEP1834493.1"
                     /translation="MFKQLFSGMFSNSDSLPITDVEQAIQSSNAILLDVREVDEFASG
                     HIPQAINQPLSQLDQFKGDKTKNYLVICQSGMRSQRATEYLKSQGYQAINVKGGMNAW
                     TGGIA"
     gene            1994..2254
                     /locus_tag="VCA20_000640"
     CDS             1994..2254
                     /locus_tag="VCA20_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEP1834494.1"
                     /translation="MNTDKKKMINRLKRAEGQLRGIQKMIEEDQECIDIVTQLSAVRS
                     SINSMMGMVIAQKVQDCIAHPSENPEEQEARLAQAINLIIKK"
     gene            2265..2822
                     /locus_tag="VCA20_000641"
     CDS             2265..2822
                     /locus_tag="VCA20_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HEP1834495.1"
                     /translation="MPTEKEKMIAGQFYMAADAELREMRTFARQQMKNFNEELDGQAR
                     SERLKNWFGATGERIYMELNFYCDYGSNIHVGEDFYANFNCTMLDVCPITIGKNAMLG
                     PNVQLLTPLHPLDPDERNSGLEYGSPITIGDNFWAGGGVTILPGVTLGNNVVVGAGSV
                     VTKSFGDNVVLAGNPARVIKEIPKK"
     gene            2881..3723
                     /gene="dprA"
                     /locus_tag="VCA20_000642"
     CDS             2881..3723
                     /gene="dprA"
                     /locus_tag="VCA20_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEP1834496.1"
                     /translation="MNNFELYKWKKAGLTNLNINKVLTYQERFGKKLSLRNIAVVSEC
                     KNPLLFMENYKNLDVKACREEFNKFSSFSILDDVYPLALKQIYNPPVLLFYQGDLELL
                     EKPKLAVVGTRNASKNGIQSVQKIIKELSNRFVIVSGLARGIDTAAHIASLKNGGQTI
                     AVIGTGLDNIYPKENKDLQTYIGKHHLVLTEYEASAQPLKYHFPERNRIIAGLVEGLV
                     VCEAKMRSGSLITCERALEEGREVYAIPGSIMDGKSDGCHHLIQEGAKCVTSGLDIIS
                     EYQF"
     gene            3816..5909
                     /gene="topA"
                     /locus_tag="VCA20_000643"
     CDS             3816..5909
                     /gene="topA"
                     /locus_tag="VCA20_000643"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEP1834497.1"
                     /translation="MATKTVSKKKTTTKKNLVIVESPAKAKTIEKYLGKNYKVVASVG
                     HIRDLKKSSMSIDFDNNYAPEYINIRGKGPLINSLKKEAKNAKQVFLASDPDREGEAI
                     SWHLAHILGLDANDKNRVVFNEITKDAVKNAFQEPRAINLDLVDAQQARRVLDRIVGY
                     SISPILWKKVKKGLSAGRVQSVALKLIIDRENEISNFKPEEYWTIDATFKKGSKKVQA
                     SFYGIDGKKTKIENNEQVKEILARLDGDDFLVEQVERKERKRNAPLPYTTSTMQMDAA
                     NKINFRTRKTMMVAQQLYEGVSLGTGGTQGLITYMRTDSTRISPVAQAQAADFITERF
                     GATYSKHGNKVKNASGAQDAHEAIRPSNVNLTPESIAKYLDKDQLRLYTLIWNRFVAS
                     QMAAAVFDTMSVRLGQNGIVFAANGSQVKFDGYMAVYNDADKNKMLPDMEQGDTVVRA
                     STNPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTIETIQKRYYVKLAAKRFEPT
                     ELGEIVNKLIVEFFPDIVNVKFTADMEQKLDDVEIGKEQWQKIIDGFYQPFKVELSKA
                     EEQMEKIQIKDEPAGFDCDVCGHEMVIKLGRFGKFYACSNFPDCRNTKQITKEIGVTC
                     PVCQKGQVIERKSKRNRLFYGCDRYPECEFTSWDKPVGRSCPKCGHYLVEKKVRGGGK
                     QVVCPNGDYEEDKIK"
     misc_feature    6325..6459
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            6486..6980
                     /locus_tag="VCA20_000644"
     CDS             6486..6980
                     /locus_tag="VCA20_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEP1834498.1"
                     /translation="MSEAIIAKKAELVNAVAEQMKAATSIVVVDARGLTVEQDTVLRR
                     NLRGAEVEYKVIKNSILRRAAEQAGLEGLAELFVGPSAVAFSNDAVAPAKTIYDFAKT
                     ADALEIKGGAVEGKVSSKEEIEALATLPNREGMLSMLLSVLQAPVRNVAYAVKAVAEK
                     EDAA"
     gene            7046..7414
                     /gene="rplL"
                     /locus_tag="VCA20_000645"
     CDS             7046..7414
                     /gene="rplL"
                     /locus_tag="VCA20_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEP1834499.1"
                     /translation="MALNIENIIAEIKEATILELNDLVKAIEEEFGVTAAAPVAVAAA
                     GGGAAEEAKDSFDVELTSAGDKKVGVIKVVREITGLGLKEAKELVDGAPAMVKEGVAT
                     AEAEEIKAKLEEAGASVTLK"
     gene            7610..7906
                     /locus_tag="VCA20_000646"
     CDS             7610..7906
                     /locus_tag="VCA20_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834500.1"
                     /translation="MFSEDLYYELEEMLTLGFKNIVEYERYIELKDSYEEETLDYSFS
                     IRELTNQLEVIINTKENDFPNLDECLHGEYLYLVNELKRLDCNIAGKYISLSYD"
     gene            8516..9337
                     /locus_tag="VCA20_000647"
     CDS             8516..9337
                     /locus_tag="VCA20_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HEP1834501.1"
                     /translation="MMKFEAEKLRELKCSGLETGFYDFDKLTTGLHEGQLIILAARPA
                     IGKTAFALNIAQNVARSSKKAVVFFSLETQEKILVKRLLANQEEHTEQHTITRKKTVE
                     DLKRHSIVQSQLTDFPIFIDDSVGLRVIDIRDKVGRLANKTNGLGLIIIDYLQLIQGS
                     RLENRQQEVSEIVHQLKTLAKELKVPVIALSQVCRSVEQREDKRPISSDLRELETSME
                     QDADIIAFLYRDDYYQEKTVCQAECNLTELIIRKNQNGRLGTVKLYFYKNLNRFL"
     gene            9422..10372
                     /locus_tag="VCA20_000648"
     CDS             9422..10372
                     /locus_tag="VCA20_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEP1834502.1"
                     /translation="MNDQERLLTIFLRLQSGAHLAKSQLADEFGVSEKTIQRDFSLLG
                     NFLESQPIVAAELAYDAKYHTRYLKGKSLFNKKDILVISKILLENRSLNKEENKSLID
                     SLLALVSKEEQKEVYQIIASELLNYAPLSDTQNRIDKIWEWSEMIRKELVLNIRYQSP
                     YNTEKQHTILPVSLYYDVHYFYVVAYNLTFESYMTLKLDRIIDWKISKEKKPQISYGE
                     KFRDGEIRNKRVDSFMGRELTIRVQFAYDPTIVTDQFPTARIIEHTPEGVIVEFISQD
                     TPGLKRWLLSQADALTVLSPATLVNDMKNFLKKMQQNYDK"
     gene            10444..10899
                     /locus_tag="VCA20_000649"
     CDS             10444..10899
                     /locus_tag="VCA20_000649"
                     /inference="COORDINATES: protein motif:HMM:NF012529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator DnaA"
                     /protein_id="HEP1834503.1"
                     /translation="MKSINKNQTFENFSVNDGNAWALTALKTVIKDKNYYNPVYIYGE
                     EGVGKSHLLNATNNALLSEQNQVILLSAENLTNHLVENLTYSEFDYILIDDLNLMPKD
                     IILEEQLAMLIEKNKKQLIISSTVTPKDLEVSSKLQERLQWGLTTSMTP"
     gene            11069..11599
                     /locus_tag="VCA20_000650"
     CDS             11069..11599
                     /locus_tag="VCA20_000650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HEP1834504.1"
                     /translation="MVQEVQKNDFQLSDYVVFDIATSGLSPKDSSLLGIFAIKIEKGE
                     IVERFRRIVKLQGELSEFITDVTKITNEMMQRGESAEQVLNDFQVFCGSLTLVAHNAE
                     FEVNFLNYNFQKYGFQALNQPVIDSLELVRKAYPELKRYSLTQLAQFFDFDTNNQNPE
                     FGAETTRMLLDELRVF"
     gene            11654..13432
                     /locus_tag="VCA20_000651"
     CDS             11654..13432
                     /locus_tag="VCA20_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HEP1834505.1"
                     /translation="MNKKIDSENQTIKAYFESKNEFYIPSYQRPYAWQVSQCEKLIED
                     IERHQENFDKDTQDNYFFGTILIAQETGEEHEVALIDGQQRTTTFMLLLKALLLRIKE
                     ELVKISPSSLEGERLRDKLISLKGMIIKMLYHLNEDDEYLYTKRDEYQLQAIDLKYVN
                     DSISELHKDDFVTILLGESYSGITNAVKKIENRRKDNKYTNFFKNFRYFYRELETKNN
                     LQLAEFAEHFVMYCQVIAITSYNTDQAINIFNSLNGTGLPLTPIEVIVSQTTANAKER
                     KVFEENWRSIVEKTDHSRLNLNSLITHYIFVKLAQFQSETRNPGVSAFFNKNNQLLRE
                     DVLFTMELDQILTIFESYLDSPEGMILDKFNNNLKPFATSFQFYKGCENEFLIYLIKL
                     GALLDLSESPYSSSKFKGFLEKINLKFSKKEVAPEEIISEMKQHIASEFNKQEIEETL
                     LESGVPTSLVYLNDYLYAKEKGEIFSLSNQVDIEHIMSQSGQNRENIMADAGFTDASE
                     FKEYAEKLGNKILLEADINRAIGDSWFRTKKMYTIADKKGYIGSRYVIAKSLTNYPKD
                     TWDKEDIDKATTKAAKRITDFIFNEK"
     gene            complement(13880..14179)
                     /locus_tag="VCA20_000652"
     CDS             complement(13880..14179)
                     /locus_tag="VCA20_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834506.1"
                     /translation="MNQLEFQRNHLQMDYYSESYQDFERDFYRYSNMNIPLTFLTDDT
                     LKTMATSHKNYFVLNKEKSKDNRDHFFIFEVRTLEENPLIYRYSYKNTTTYLAQK"
     gene            14553..14912
                     /locus_tag="VCA20_000653"
     CDS             14553..14912
                     /locus_tag="VCA20_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834507.1"
                     /translation="MSEQYRDIRKEINLTANELKQIEQLMREKRYRHFSPFVRDQLLM
                     TDDKQLTAKEWFSFWETQKLEQIGRDVHEIMTVARVNNQVTQEHVSILLTCVQDLIFE
                     VSQMQPLSREFREKYIG"
     gene            15095..15337
                     /locus_tag="VCA20_000654"
     CDS             15095..15337
                     /locus_tag="VCA20_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003106749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HEP1834508.1"
                     /translation="METITHKNIMKLGFSKTASKLIIREAKAIAVEQFYTSHLDNNAV
                     KLRKSPFDNRRLDLAPKVIVEELLGFPITDERKENR"
     gene            15338..16498
                     /locus_tag="VCA20_000655"
     CDS             15338..16498
                     /locus_tag="VCA20_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEP1834509.1"
                     /translation="MSKKYKGVFRDDKGRIYCQTEFKVSATGKRQRFKRYRNMWGKSF
                     TTEKEAYDELCRARVEFYDKFDYSDYNLTFAQYMEDIFLPYYRQTVQDSTYRTAITHF
                     TLFIEEFGKMKLRDIKPRDCERFRLKLIANHSPNYAKCVWIRFKQCLGYAERMEYIKT
                     FPCKSLDNPKGKRPDTKFWTFDEFKKVIATFDIEDYEDLHRFTTTWLYYMTGVRVSEG
                     FALLWSDYDKENKRIFVHSTLEALKGGIYRIKDQTKTDAGVRFVDLDDETINVLERWR
                     KVQVANSDDNYILSKFGEPMVKSTLTRMLKRQAKLAGVPEITGKGLRHSHDSFMINVL
                     GKDVLAVSQRSGRIDKATTLNTYSHFYNARKQTVGNEITELLKKEGVSSTPR"
     gene            16839..17012
                     /locus_tag="VCA20_000656"
     CDS             16839..17012
                     /locus_tag="VCA20_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834510.1"
                     /translation="MQKNEQSSRQIVMRHLMAIMRIDIEKAKRIVAEMEEKGLIQFDE
                     LGNVGLLVLEVQS"
     gene            17009..17833
                     /locus_tag="VCA20_000657"
     CDS             17009..17833
                     /locus_tag="VCA20_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEP1834511.1"
                     /translation="MKRITANHYQTSERYYKLPKLLFESERYKDMKLEVNVAYAVLKD
                     RLELSLSRGWIDEDGAIYLVYSNSKLMALLGCSKSKLLTIKKTLREYGLIDEVQQSSS
                     EKGRLANKIYLGELEHEPTPVSNSDGGSVQKTLGRCQNQPGPVLNLATSETEVSETDM
                     SETKESESVIEDEEEKESLTSKKSDNDNFQRKVDRVTRYDRDYIWGLVHDQLRQVGLS
                     RAASDYAMLYFDHRYQYALENMRFADRTDTIAEYVFNGVLSEWTKVQRLKELKGGE"
     gene            18028..19383
                     /gene="dcm"
                     /locus_tag="VCA20_000658"
     CDS             18028..19383
                     /gene="dcm"
                     /locus_tag="VCA20_000658"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEP1834512.1"
                     /translation="MKFLDLFAGIGGFRLGLTRQGHKCIGFCEIDKFARKSYKAIYET
                     EGEIEFHDIRQVTDQDFRQLRGQVDIICGGFPCQAFSLAGRRLGFEDTRGTLFFEIAR
                     ATKQIQPRFLFLENVKGLLSHDKGETFRTILATLDELGYDVEWQVLNSKDFQVPQNRE
                     RIFIIGHSRRYRPRFLFPLRGENSSAGLERLGNVNPSSKGMNGEVYLSRGIAPTLTRG
                     KGEGTKIAIPVLTPDRLEKRQHGRRFKGNDDPMFTLTSQDRHGVVVAGTLPTSFIQTG
                     RVYDLSGLSPTLTTMQGGDKVPKILCREEAPHLKIREATKLGYAKAIVGDSVNLAYPE
                     STKRRGRVGKGISNTLTTSDNMGVVVAAMEYRKDKWYEVTGIILDGKLYRLRIRRLTP
                     RECFRLQGFPDWAFDKAESVSSKSQLYKQAGNSVTVTVIEAIAREFRKIEEEEEHEII
                     T"
     gene            19367..19795
                     /locus_tag="VCA20_000659"
     CDS             19367..19795
                     /locus_tag="VCA20_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834513.1"
                     /translation="MKLLHSKSIRNCTELEEAIHQAEVERFSEMIASLPNYDCDIDVT
                     FEDDYHKEMNSPLAYESNLHRIFEFIETQDIKNGVDTYLTDENNLAFRAYGQGYSWND
                     KNDVVTTLITVKCFGEGRSPIDMSKVFTSPTQALEKELSV"
     gene            19805..20188
                     /locus_tag="VCA20_000660"
     CDS             19805..20188
                     /locus_tag="VCA20_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834514.1"
                     /translation="MLEIYLSRNNSRNQKLLSFCDSHGISYSCKEVSHLSREELLRLF
                     TKSSDCFTLLSPSLQRFKGHREMKLSELVTLVLRKPDQNLRLPLVVCEDNVYPDMSLE
                     EARTFLPRNQKVVSFREHLFKDLVT"
     gene            20198..20431
                     /locus_tag="VCA20_000661"
     CDS             20198..20431
                     /locus_tag="VCA20_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003065178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEP1834515.1"
                     /translation="MNDRFWENLEIIVMEKGLTWADLARQMFKGQYVYPSEFNRLYQT
                     FRHYKSHRLMPQGKWVEKIVSVLEIDYEDLFRR"
     gene            20434..21015
                     /locus_tag="VCA20_000662"
     CDS             20434..21015
                     /locus_tag="VCA20_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018165725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEP1834516.1"
                     /translation="MKRLVVMYGAIHVNVLLVSFYLVGWLNGAWLHVLQVTFLALLLW
                     GWKRFKIQKRNLSFKERGLWVCGSLGVMASMALIMSGLFSGSEPNQETLVTVQDQIPV
                     LSFILFLLNASVVEEVFYREVLWGVLSQPVVQVLLTSSLFALAHHPSSLFTWVLYGSL
                     GLTLGVVRGQTDCLTSTLIHLSWNGIVFFLSLL"
     gene            21092..21580
                     /locus_tag="VCA20_000663"
     CDS             21092..21580
                     /locus_tag="VCA20_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834517.1"
                     /translation="MSSEQQERQAIQYAERSAMFTVKVLLKLLEWSARHALAQDSAYK
                     VGVQKLEELLQSPYAIESLNISKDILDKPVDVEKFKELVESENLPIAISWQGDYLHFY
                     AKDKSLLDKHLNELLQKLVSNPEKLKGLTLDKTLDEEIAQAKEQIVMSEPSAVKSKEV
                     IL"
     gene            21580..>22787
                     /locus_tag="VCA20_000664"
     CDS             21580..>22787
                     /locus_tag="VCA20_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003086094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HEP1834518.1"
                     /translation="MVSGKKVFIFGVLGLALGYFCHRLVLLYDSLPNQPPLERLAYLL
                     GEGQNQVLNPLWNGHFTSRSVLGFCFGFVTMGLVYLYVSTGQKVYREGAEYGSARFGN
                     SHERKAFISKNPFNDTILSRNVRLTLLEKKAPQFDRNKNLVVIGGSGSGKTFRFVKPN
                     LIQLNCSNIVVDPKDHLAEKTGKLFLAHGYQIKVLDLVNMTNSDGFNPFRYVETENDL
                     NRMLTVYFNNTKGNGSRSDPFWDEASMTLVRAIASYLVDFYNPPGSTKEEADSRRKRG
                     RYPSFSEIGKLIKLLSKGENQDKSVLEVMFETYAKTYGTENFTMRNWADFQNYKDKTL
                     DSVIAVTTAKFSLFNIQSVIDLTKRDTLDLKTWGTQKTMVYLVIPDNDTTFRFLSALF
                     FSTVFSTLTR"
BASE COUNT         7394 a         3793 c         4870 g         6730 t
ORIGIN      
        1 accattttct tttaagatac gttcggcaat ataagaacgc agtccactat ggcagctgac
       61 aatgtattct tttgaagcat ccagttctcc aattcgctca cggagttggt ccaaaggaat
      121 gtgaatgaat tctctgaatt gcccatttgc gacctcacca ggattgcgga catctaatag
      181 aactttacca gtagctaact cgtcgtctag ttgatgccac tggatgtttt ggctaatccc
      241 ttctgcgatg ttcatagcag cgtagccaat catattgaca gggtctttgg cagagcgtag
      301 gtcaattcta gctctggtaa atcaaaaatt gtcagtccag ccttgatagc agtggcaagg
      361 acattaatcc gcttatcaat acctttttga ccgacggctt gcgctccgaa aatttttcca
      421 tcagtcggtg agaaaatgag ttttaaaaca atatcagtcg caccaggata ataacctgca
      481 tggtcttttc cagttgtatg aacgacggca tgtttatagc cgagtgattg atcagcacgt
      541 tcgcttaaac cagttgaagc ggcagctagg tcaaagactt ttacgatagc tgttccaata
      601 gatcctttgt tttttctatc aagtccagca atgacatcag ctacctgtct tccctgacgg
      661 ttagcaggag aagccagtga aatcaaggca tcttgacctg taatttcttg tttaacaaca
      721 atagcatcac caacagcata aatattcttc tgactagttt ggtagttttg gtctacgaga
      781 ataccaccac ggagacctaa ctcaactcca gcatctttgg ctagctgact gtcgggagca
      841 actccgactg acagaatggt cacatcagat gatagggtct gtccgttaga caattcgact
      901 acttgaccat gttcgtaaaa agcagtgaca gactgcccag tgataacctc gacaccctta
      961 cttgttaact ctgcttccac aaatcgagcc atctctaggt caaatgttgg caaaacatga
     1021 ggtgcctgtt caacaagtgt gactttcagg cctttcaagg caagattttc agccatttcc
     1081 agaccgataa atcctgcacc aattaccaca gcatgaccag acttgatctg gtctagttta
     1141 atcataatct tgtccaaatc aggtacgttc cgaagagtaa agagattgtt tgcttcctca
     1201 atacctgata gatttggaat gacaggcttg gcgccgggtg ataggattag tttgtcgtag
     1261 ccttcaatca acttaccagt aggcgttttg actgttacag tttgctgctc agcatcgata
     1321 ctaatgactt catggaaggg gcggacatct agattaaagc gttgtttcaa actttctggc
     1381 gtttgaagca ataaactgtc atgctctgct atttctccag aaatgtaata aggtaaaccg
     1441 cagttggcaa aggaaacata aggtcctttc tcaaaaacga tgatttcagc attttccatc
     1501 aaacgacgga gacgagtagc tgctgacata ccgccagcga caccacctac aataattatt
     1561 ttcatgctat tcctcctgtc caggcattca taccgccttt aacgttgatt gcttgatagc
     1621 cttgagattt aagatattct gtcgcccgtt ggctacgcat gccagattga cagatgacaa
     1681 gatagttctt ggttttgtcg cctttaaatt ggtcaagttg acttaagggc tggttgatag
     1741 cttgtggaat atgtccacta gcaaattcgt cgacttcgcg aacatcaagc aaaatagcat
     1801 tcgatgattg tatcgcttgt tctacatcag ttatcggaag actatctgag tttgaaaaca
     1861 ttcctgaaaa taattgttta aacattatta ctccttttta tacccatcgg ggtatctaaa
     1921 atatatcaag gaattcaaaa gatgtcaagg taaaaaattg acatttttca cttcaagcag
     1981 taccatatag ggtatgaata ctgataagaa aaagatgata aaccgtttga aacgggccga
     2041 aggtcaactc agaggcattc agaaaatgat tgaagaagat caggaatgca ttgatatcgt
     2101 aactcagttg agtgctgttc gttccagcat taatagtatg atgggaatgg ttattgctca
     2161 gaaggtacaa gattgtatcg ctcatccgtc tgaaaaccct gaagaacaag aagctcgtct
     2221 tgctcaagcc atcaatttaa tcataaaaaa ataggagcag aagtatgcca acagaaaaag
     2281 aaaagatgat agctgggcaa ttttatatgg cagcagatgc tgaattgaga gagatgcgaa
     2341 cctttgcccg ccaacagatg aagaacttta acgaagaact agatggacaa gcccgctctg
     2401 agcgattgaa aaactggttt ggagctacag gtgagcggat ttacatggag ctaaatttct
     2461 actgtgacta cggtagcaat attcatgttg gtgaggattt ttacgcaaat tttaactgta
     2521 cgatgttgga tgtttgtccc attaccattg ggaaaaatgc catgcttggt cccaatgtac
     2581 agttgctaac tccacttcat ccactagatc ctgatgaacg aaattctggt ttggaatatg
     2641 gctctccaat taccattggt gataatttct gggctggtgg tggtgtgaca atcttacctg
     2701 gtgtgacctt gggcaacaat gtggttgttg gtgctggttc tgtcgtgact aagtcttttg
     2761 gtgataatgt cgttttagcg ggcaatcctg cgcgtgttat caaagaaata cctaaaaaat
     2821 aattcttctt cgggagaatt ttttctttgc cttgcgaata gagtagatga ggtgaagaaa
     2881 atgaataatt ttgaattgta taaatggaaa aaagcaggtc tgaccaatct caacatcaat
     2941 aaggtattga cttatcaaga aagatttggc aaaaagctat ccttgcgcaa tatcgctgtc
     3001 gtttctgaat gtaaaaatcc attgttattc atggaaaact ataagaattt agatgtcaaa
     3061 gcctgtcggg aagaatttaa caagttttct tctttttcga ttttggatga cgtctatcca
     3121 ctagcattga agcaaattta caatccacct gtacttttat tttatcaagg ggatttagaa
     3181 ttgctggaaa aaccaaagct ggcagttgtt ggtacacgaa atgccagtaa aaatggtatt
     3241 caatctgttc agaaaattat caaagaattg agcaatcgtt ttgtcattgt tagtggtttg
     3301 gcaagaggaa ttgatacagc tgcccacatc gccagtttga aaaatggagg ccaaaccatt
     3361 gctgtaatcg gaacaggttt agataatatt tatccaaaag aaaataaaga tttgcagacc
     3421 tatattggaa aacaccattt ggtgctgaca gagtatgaag ctagtgctca gccactcaaa
     3481 taccattttc cagaacgcaa tcgcatcatt gctgggttgg tagaaggtct ggtagtctgt
     3541 gaggcaaaaa tgcggtcggg tagcttgatt acctgcgaac gtgccttgga agaaggaaga
     3601 gaagtctatg ccatacctgg ttccattatg gacggaaaat cagatggttg tcatcatctg
     3661 attcaagagg gagcaaagtg cgttacatca ggcttggaca tcatttctga gtatcaattt
     3721 taaaacaagt cttcctatag gaaaataaat atcttgacac tgactgaaaa atagtatatt
     3781 ctttgtgagg ttaaaaatat ttgtaaggta taagaatggc tacaaaaaca gttagtaaga
     3841 aaaaaacaac aaccaagaaa aatctcgtta ttgtagagtc gcctgctaag gcaaaaacaa
     3901 tcgaaaaata tcttggtaaa aattataagg tcgttgcttc agtcgggcat attcgagatt
     3961 tgaagaaatc tagtatgtct attgactttg ataacaacta tgcacctgag tacatcaaca
     4021 tacgtggaaa aggaccactc atcaattctt tgaaaaaaga agcaaaaaat gctaagcaag
     4081 tctttctcgc gagtgacccg gaccgcgaag gagaagcaat ttcttggcat ttggctcaca
     4141 ttttgggatt ggacgcaaac gacaaaaacc gtgttgtctt taatgaaatt acaaaagatg
     4201 cagtaaaaaa tgcctttcag gagccacgtg ctattaacct agacttggta gatgcccagc
     4261 aagctcgtcg cgtcttagac cgtattgtag ggtactccat atcacctatt ttatggaaga
     4321 aagtcaaaaa aggtctgtca gcaggacgtg ttcaatcggt tgctctcaaa ttaataatcg
     4381 accgtgaaaa tgaaatcagc aatttcaaac cagaagaata ttggacaatt gatgcaacct
     4441 tcaaaaaagg gagcaagaaa gtccaggctt cattctacgg tatagatgga aagaaaacta
     4501 aaattgaaaa caatgagcag gttaaagaaa ttctcgcccg acttgatggg gatgatttct
     4561 tagtagaaca ggttgagcgt aaggaacgca aacgaaatgc acctctgcct tacacaactt
     4621 ctactatgca gatggatgcg gctaataaaa tcaatttccg tactcgtaaa accatgatgg
     4681 ttgctcaaca actttatgag ggtgtcagtc ttggcacagg tggtacccaa ggtttgatca
     4741 cctatatgcg tacggattca acacgtatta gccctgttgc acaagcacaa gctgctgact
     4801 ttatcacaga gcgttttggt gctacctatt caaagcatgg taataaagta aaaaatgcca
     4861 gtggtgcaca agatgcccac gaagccattc gtccatcaaa tgttaactta acacctgaat
     4921 caattgcgaa atatctggac aaggaccagt tgcgactata tacactgatt tggaatcgtt
     4981 ttgtcgcaag tcagatggct gcagcagttt ttgataccat gagtgttcgt ttgggtcaaa
     5041 atggtattgt ctttgctgct aatggtagcc aagtaaaatt cgatggttat atggctgtct
     5101 ataacgacgc tgataaaaat aaaatgttgc cagatatgga acaaggtgac acggtggtac
     5161 gcgcgtcaac aaatccagaa caacacttta ctcagccacc agctcgctac tctgaagcaa
     5221 ccttaattaa aacattagaa gagaatggtg taggtcgtcc gtcaacctat gcaccaacga
     5281 ttgaaaccat tcaaaaacgt tactatgtta agcttgcggc taagcgattt gaaccaactg
     5341 aactcggaga aatcgttaat aagctgattg ttgaattctt cccagatatt gtcaatgtca
     5401 aatttacagc cgatatggaa caaaaattgg atgatgtaga aatcgggaaa gagcagtggc
     5461 agaagatcat tgacggtttc tatcagccat tcaaggtaga attatctaag gcagaagaac
     5521 agatggaaaa aatccaaatt aaggatgaac cagctggctt tgactgtgat gtttgtggac
     5581 atgaaatggt tattaaatta ggacgttttg gaaaattcta tgcatgtagc aatttcccag
     5641 actgtcgcaa taccaagcaa atcactaaag aaatcggtgt gacttgtccg gtttgccaaa
     5701 aagggcaagt gattgaacgc aagagtaaac gcaatcgtct tttctatggt tgcgatcgct
     5761 acccagagtg tgaatttact tcttgggaca agccagtcgg gcgttcttgt ccgaaatgcg
     5821 gccactatct cgttgagaaa aaagttcgtg gtggtggtaa acaagttgtc tgtccaaatg
     5881 gtgactacga agaagacaag attaaataag attaaactgc tattctcaag agaatggcag
     5941 ttgtttttta gagtcatgtt tggtttatct tagcttactt cgacaagcga ggaaacttcg
     6001 tctccttatt ttcaacttta aacgaggctg gtcaaaaagt ccaaagtcac ttctcagtgc
     6061 aatggttgat tggatttaac aggccagtgg agtgaaactg ttcagtgaac tgtttcagcc
     6121 tgagcttaga aatgaaaaaa tcgaggtaaa aaggttggag ataagatttg cgaagcaaat
     6181 cacagcaatt cgtgtttcac attccaagtc tgacctctac aactaaaacg aactaaacgg
     6241 caataccgct tgaaaaaata ttgaaaaaat taaaaaaata ggagaaaagt cttgcattat
     6301 tgaaaatact gtgttatgat aattgaggtt tgaaaaaact gacctaagac agtaggggag
     6361 ctggactcat aaacatccta ccgaggcaca aaacgattat tgagataaac gtatttgtac
     6421 tttcgtgtct aggtttaggt gcgatttttt tgtgcctagc ccaaaataaa aacggaggta
     6481 aaaaaatgag tgaagctatt atcgctaaaa aagcggaatt agtaaatgcc gttgctgagc
     6541 aaatgaaagc tgcaacatct atcgttgttg tagacgctcg tggtttgaca gtagaacaag
     6601 atacagttct tcgtcgtaac ttgcgtggtg cagaagttga gtacaaagtt atcaaaaact
     6661 ccatcttgcg tcgtgctgct gagcaagctg gtcttgaagg tcttgcagaa ttgtttgtag
     6721 gtccatctgc agttgcattc tcaaacgatg cagttgctcc agctaaaaca atttacgatt
     6781 tcgcgaaaac tgctgatgct cttgaaatca aaggtggagc tgtagaaggt aaagtttctt
     6841 ctaaagaaga aatcgaagca cttgctacat tgccaaaccg cgaaggcatg ctttctatgt
     6901 tgctttctgt acttcaagcg ccagttcgca acgttgcata tgctgtcaaa gctgttgcag
     6961 aaaaagagga cgcagcttaa tgcgtcagag cgtagctgct tagtgctaca aactatttaa
     7021 aaacctaaat tggaggaaaa tcacaatggc attgaacatt gaaaacatta ttgctgaaat
     7081 taaagaagct actatccttg agcttaacga ccttgttaaa gctatcgaag aagaatttgg
     7141 tgtaactgcg gctgctcctg tagctgttgc tgcagctggt ggtggtgctg ctgaagaagc
     7201 taaagattca ttcgacgttg aattgacatc tgctggcgac aaaaaagttg gcgttatcaa
     7261 agttgtacgt gaaatcactg gtcttggtct taaagaagct aaagagcttg ttgacggtgc
     7321 accagctatg gttaaagaag gcgttgcaac tgcagaagct gaagaaatca aagctaaatt
     7381 ggaagaagca ggcgcttcag ttactcttaa ataatcaatt atcgattgtt taaggctgaa
     7441 agccgagttt taaagccttt tcgctaaagt caaggtgatt aagaagggat aatttcaatt
     7501 gaaatttccc cacaaacacc cccacaaatt tttgtggggg tgttttgttt ctagctgaca
     7561 ttctatattt gttgcggtaa actaatgaca aatacagagg agtgaactta tgtttagtga
     7621 agatttatat tatgagttag aagaaatgtt aactcttggt tttaaaaata ttgttgaata
     7681 tgaaagatat atagaactta aggactcata tgaagaagaa actttagatt atagtttttc
     7741 aatcagagaa ttaactaatc aacttgaagt tattatcaat accaaagaaa atgattttcc
     7801 taatttagat gaatgcttac atggagaata tttgtattta gttaatgaat taaaaagatt
     7861 agattgtaat atagctggaa aatatatcag tttatcttat gactgagaga tgtatgaagt
     7921 tgcaattatt ctttcttata gaaaaaattt attttgttga tttgttccca attacacatc
     7981 acgtagagtg tgttgctctg ctcgtaaaag cctgaaacct ttgaacgaaa gcatcttaaa
     8041 aagccttgga gacaaggctt tttagcttgg ggtggggaag tatcagagtg agaaaatttt
     8101 tgagtgggta gaagtgatat ctagaaatta ttagtttcta tttccattta tcctgtgggt
     8161 acgtgtttgt ttccattgac aaggagtttg tgggaataga aatgtaccca ccctattgat
     8221 gaaaccaagt aagctttagt ctagggaaat taagtagaag aaaagtgata ttaacaatat
     8281 taaaaagcac cttgaaatgc ttggtttcaa ggtggattat aattgcatga attggttcat
     8341 ttattaacgt ttacttgttt cgaaagaagt gctgtattac tgaattttat agtggaattg
     8401 ttaggattat ttattaatat ttgaaaggtt agaataaaat cattataacg gacaattact
     8461 gtccattctt ttttgtaaac tcatatcatc aataaactat tagaggtggt caaaaatgat
     8521 gaaattcgaa gctgaaaagt tgagggaatt aaaatgtagt ggcttggaga caggctttta
     8581 tgactttgat aagttaacaa ctggtttgca tgaggggcag ctaatcatct tggcagctcg
     8641 tcctgcaatt ggaaaaacag cttttgctct aaatatagct caaaatgtgg ctcgaagttc
     8701 gaaaaaagct gtagtattct tttctcttga gacgcaggag aaaatcctcg taaaacggct
     8761 cctagctaat caggaggaac acacagagca acataccatt acacggaaaa aaacggttga
     8821 ggacttgaag aggcattcca ttgttcagag tcagctgaca gattttccta tttttattga
     8881 tgactcagtc ggattaaggg ttatagatat tagggataag gtagggcgtt tagccaataa
     8941 gacgaacggg ctagggttaa ttatcattga ctatctccag ctaattcaag gatcacggtt
     9001 ggaaaatcgt cagcaggaag tatctgaaat cgtacatcag ttgaaaaccc ttgctaagga
     9061 attgaaagtg ccagttatcg ctctatctca ggtctgtcgt agcgttgagc aacgggagga
     9121 caagaggccg atttcgagtg atttgcggga gttagagact agtatggagc aggatgctga
     9181 tattatcgct tttctctatc gtgatgacta ctaccaagag aaaacagtct gtcaggcgga
     9241 atgtaatctg acggagctaa ttattagaaa gaatcagaac gggagacttg ggacggtaaa
     9301 gctgtatttt tacaagaatt tgaatcgttt tttgtaaaaa tattaacctt gaaaaagtcg
     9361 ggaaatcatt gaatagggac tacttgaggt gttataatgt ttctaaggaa ttgaggtaaa
     9421 tatgaacgat caagaacgct tactaacgat ttttctacgt ttgcaatcag gtgcccatct
     9481 agcaaaatca cagctcgctg atgagtttgg agttagtgaa aaaacgattc agagagattt
     9541 ttctcttttg gggaatttct tggaatcaca gccaatagtt gcagcggaac ttgcctatga
     9601 tgcaaaatac catacccgtt atctaaaagg aaaatcatta tttaataaaa aggatatttt
     9661 ggttatatca aagattttgt tagagaaccg ttctttgaac aaggaagaaa ataagtctct
     9721 tatcgatagt ttattagctt tggtttctaa agaggaacaa aaagaggttt accagattat
     9781 tgcaagtgaa ttgctaaatt atgcgccatt gtctgatact caaaatcgaa ttgataaaat
     9841 ttgggaatgg tcggaaatga ttcgaaaaga gttggttcta aatatccggt atcagtcacc
     9901 ttataatact gaaaaacaac atacgattct tcctgtatcc ctttactatg atgtacatta
     9961 cttttatgtt gtagcataca atttgacttt tgagtcgtat atgaccttaa aattggatag
    10021 aattatagat tggaaaatat caaaagaaaa gaaaccacag atttcttatg gggagaagtt
    10081 tcgtgatgga gaaatacgga acaaacgtgt tgattccttt atgggacgag agttgacgat
    10141 tagagttcaa tttgcctatg accctaccat tgtaacggat caatttccaa cagccagaat
    10201 cattgaacat acacccgaag gcgttatcgt tgaatttatc agtcaagaca caccaggatt
    10261 gaaacgctgg ctgctaagtc aagcagatgc tttgactgtg ctttctcctg caacgttagt
    10321 aaatgatatg aagaattttc tcaaaaaaat gcaacaaaat tatgacaaat agaataggtg
    10381 gacaaatatt gtccacgatt ccttttataa taaacgtatc aagttataaa tgaggtacaa
    10441 aagatgaaat caatcaacaa gaatcagaca tttgagaatt tttcggtcaa tgatggaaat
    10501 gcttgggctt taactgcatt aaagacagtt attaaggata agaattacta taatccggtt
    10561 tatatatacg gtgaagaggg agttgggaaa tctcatttgc taaatgctac aaacaatgca
    10621 ctgctatctg aacaaaatca ggtaatctta ttatcagcag aaaatttaac caatcattta
    10681 gtagaaaatc tcacctattc cgaatttgac tatatcttaa ttgatgattt gaatcttatg
    10741 cctaaagaca tcattctaga ggaacaactc gcaatgttga ttgaaaaaaa caagaaacag
    10801 ctaattattt catcaactgt cactcccaaa gacttagaag ttagtagtaa attacaggaa
    10861 agattacaat ggggactaac aacaagtatg acaccctaaa atcaatgatt agtaaattta
    10921 aataggaagg agaaatctaa catgcaagaa tggattgttg ttgatcagtt tggtaatatg
    10981 attgcacaag ggttctatga taaacaatca gctgaatttt atgcaaaaaa tattccaaat
    11041 gctagtgtag tgaagaagta ggtgaaaaat ggttcaggaa gtacaaaaaa acgatttcca
    11101 attaagtgac tatgtggtat ttgacatcgc gacatcaggt ctttctccaa aggactcatc
    11161 tttgcttgga atctttgcta ttaagattga aaaaggagag atagttgaac ggtttagaag
    11221 aattgtcaaa ttgcagggcg aattatcaga atttataact gatgtgacga agataaccaa
    11281 cgaaatgatg cagagaggtg aatcagcaga gcaagtgtta aatgattttc aagtattttg
    11341 tggtagtttg actcttgttg cccataatgc tgagtttgaa gtgaattttt tgaattataa
    11401 ttttcaaaag tatggttttc aagccttgaa tcaaccagtg attgattcac tagaattagt
    11461 acgaaaggct tacccagaat taaaacggta ttctttgact caattagcac aattttttga
    11521 ctttgataca aataatcaaa atccagaatt tggagctgaa acgacacgaa tgcttcttga
    11581 cgaactcaga gtgttttgat aaagttaaga cgagttctaa ttttggattg aagataagtt
    11641 gaaaggatta acaatgaaca aaaaaatcga ttcagaaaat caaacaatta aggcttattt
    11701 tgagagtaag aatgaatttt acattcctag ctatcaacgt ccatatgctt ggcaagtatc
    11761 ccaatgtgaa aaattgatag aagatatcga acgtcatcag gaaaattttg ataaagatac
    11821 tcaagacaat tatttctttg gtactatttt gattgcacaa gaaacaggtg aggagcatga
    11881 agtggcttta attgatggcc agcagaggac aacgactttt atgttgcttt tgaaagccct
    11941 gctcttgaga ataaaagaag aattggttaa gatttcaccg tcttctcttg aaggtgagcg
    12001 tttgcgtgat aaattaatat ccttaaaggg gatgattatc aaaatgcttt atcatctaaa
    12061 tgaagacgat gagtatcttt atacgaagcg agatgagtac caacttcaag ccatagatct
    12121 taaatatgtc aatgattcaa tttctgaact gcacaaggat gacttcgtga cgatattgtt
    12181 gggtgaaagt tattctggca ttaccaatgc tgttaaaaaa attgaaaacc gacggaaaga
    12241 caataagtac acaaattttt tcaagaattt ccgatatttt tatagagaat tagaaactaa
    12301 aaataattta caactcgctg aatttgctga gcattttgtt atgtattgtc aggtcattgc
    12361 cattaccagt tataatactg atcaggccat taatatcttt aattcattga acggtacagg
    12421 cttgccatta acacctatcg aagtcattgt ttctcaaaca actgctaatg ctaaagaacg
    12481 aaaggtcttt gaagaaaact ggcggagtat tgttgagaaa actgatcatt cgcgtttaaa
    12541 tttgaacagt ttaataacgc actatatctt tgtcaaattg gcacaattcc aatctgaaac
    12601 gagaaaccca ggggtatctg cttttttcaa taagaataat cagttgttaa gagaagatgt
    12661 tctattcaca atggagttag atcaaatatt aactattttt gagtcttatt tagacagtcc
    12721 ggagggtatg attcttgata aattcaataa caatttaaaa ccatttgcca cgagttttca
    12781 attttacaaa ggttgtgaaa atgagttttt gatttactta ataaaacttg gtgcattact
    12841 tgatttgtca gaaagtcctt atagtagttc aaaatttaaa ggcttcttgg agaaaattaa
    12901 tcttaaattt agtaagaagg aggtcgctcc agaagaaatt atttcggaaa tgaagcaaca
    12961 tattgctagt gaatttaata agcaggaaat tgaagagacg ttattggaga gtggtgttcc
    13021 aacatctttg gtctatttaa acgactacct ttatgcaaag gaaaaaggag aaatttttag
    13081 tctctcaaat caagtagata ttgaacatat tatgtcccaa agcggtcaaa atcgagagaa
    13141 tattatggca gatgctggtt ttacagatgc ttcagaattt aaagagtatg ctgaaaagtt
    13201 ggggaataaa attcttttag aagctgatat caatcgagca attggggata gttggtttag
    13261 aaccaaaaaa atgtacacga ttgcagacaa aaaggggtat attggaagcc ggtatgtaat
    13321 tgctaaatca ttaacaaatt atccaaaaga tacgtgggat aaagaagata tagataaagc
    13381 aacgacgaag gcagctaaac gaattacaga ttttattttt aatgaaaaat gaatgtaaaa
    13441 gtcgacagta actgagcgag gataacttta gtttagcatt tgaattaaga tagtttcaat
    13501 gatactaact ataggaatgc taaagtctgt tttaggtttt aatccataac tgtaacattg
    13561 gaaacatggt ttccatatac ttaatatgat aaaggaaaaa gccttacaag gctttcagaa
    13621 cgtagacaaa ctcctaaaaa gataaaaaat tgaagaaatc tgcgaaagat agcaagtcat
    13681 taaactctta gaaaccttgc tatttcggtg tttctgagag ttttttattt tcaacataaa
    13741 gaaaaaagat tgaagaaaat tgtccaaaat ggactttttc ttcaatctga aagccttaca
    13801 aggctttttt ctttgagtga ggagtataaa aaacaatcaa taactctatt tccatatacc
    13861 tagatttatg tctaagattc tatttttgtg ctaagtaagt tgttgtattc ttgtaagaat
    13921 atcggtagat tagtggattc tcttctaagg ttcttacttc aaatatgaag aagtgatcgc
    13981 ggttatcttt ggacttttcc ttattgagga caaagtaatt cttatgtgaa gtcgccattg
    14041 ttttgagggt atcatcggtc aggaaggtca atggaatatt catgttggag tatcggtaga
    14101 agtcacgttc aaaatcttgg taactctcgc tgtaatagtc catttgcagg tgattacgct
    14161 gaaactcaag ctgattcata aagcacctcc tcaacaagtt caatactaat aataagttca
    14221 ttataaagta ttaaagagag catttagttc tctttttatt atgctttatt gagtgatgtg
    14281 ataacttttc caaatcatcc ttgttatcct aatatcattc ctaagaaaat caagtcttga
    14341 ttttttgttg ggggttgggg ggcaaagcca ccaagttatc ttatcgttgg ctgtcaaaac
    14401 tggaaagttt tggtctgctg gcgatatgat tttttgggta ttgtgaccac aatgcctgag
    14461 ctcgcaaaga cccaacaagg tctttaggga atttcccgta tgacatacgg ggtcggggat
    14521 tacccgagaa aggataaaaa ggagaaaata acatgtctga acaatataga gatattcgaa
    14581 aagaaataaa tttaacagcc aatgagttaa aacaaattga acaattaatg agggagaagc
    14641 gttatcgaca cttttcacca tttgtcagag atcaattatt aatgactgat gataaacagt
    14701 taactgctaa agagtggttt tcattttggg aaactcaaaa gttagaacag attggacgtg
    14761 atgttcatga aattatgact gtcgcaagag tgaataatca agtgactcag gaacatgtat
    14821 ctattttatt gacctgtgtt caagatttaa ttttcgaagt tagtcagatg caaccactga
    14881 gtagagagtt tcgtgaaaag tatataggtt agtagattta attttatagt gtatagctgt
    14941 tttaatccta gggatttact tactttaatt ttaagtaatg aaagttttaa agtagtattt
    15001 tagaaatgac tgtactgtga taactttttc aaacataaat tggtacttta ctattatctg
    15061 agagtttatc agaataaaat agaaggagta tccaatggaa actattacac ataaaaatat
    15121 tatgaaactt ggcttttcta agacagcatc aaagctgatt attagagaag ctaaagcaat
    15181 agctgttgaa caattttata ctagtcattt agataacaat gcggtaaaat taagaaaatc
    15241 gccttttgat aatagacgat tagacttagc tcctaaagtt atcgtagagg agttgctcgg
    15301 ttttccaata actgatgaaa gaaaggaaaa cagataaatg tctaagaaat ataaaggtgt
    15361 atttagagat gataaaggta ggatatattg tcaaactgaa tttaaggtat ctgcaactgg
    15421 gaaacgtcaa cgttttaaac gttatcgaaa tatgtgggga aaatctttca cgacagagaa
    15481 agaagcttat gatgagcttt gtcgagcaag agttgagttt tatgataaat tcgattattc
    15541 agattataat ttaacatttg ctcagtacat ggaagatatt ttcttaccgt attatagaca
    15601 aacggtacag gattcaacct atagaactgc aatcactcat tttactttat ttatagagga
    15661 attcggtaaa atgaaattgc gtgatattaa gccacgtgat tgcgagagat ttcgattgaa
    15721 acttatagca aatcattctc ctaattatgc aaaatgtgtt tggatccgtt tcaaacaatg
    15781 tttaggatat gcggaaagaa tggaatatat taaaactttc ccatgtaaga gtcttgataa
    15841 tccgaaaggg aagcgaccag atacaaaatt ttggacattc gacgagttca aaaaagttat
    15901 tgcaaccttt gatatagaag actatgaaga tttacatcgc tttacgacaa cttggttata
    15961 ctacatgacg ggggtgcgag tcagtgaagg ttttgcttta ttgtggtctg actatgataa
    16021 agagaataag cgaatttttg tccattcaac tttagaagcg ttaaaaggtg gtatttatcg
    16081 tataaaagac cagactaaaa cggatgcagg ggttcgattt gttgatctag atgatgaaac
    16141 gattaatgtc ttagaacgtt ggagaaaagt tcaagttgct aattctgatg ataactacat
    16201 cttatctaag tttggtgaac ctatggttaa gagtacctta actagaatgt tgaagagaca
    16261 ggctaagtta gcaggagtac ctgagataac tggtaaagga ttgagacata gtcatgattc
    16321 atttatgatc aatgttctag gaaaagatgt tttagcagtc tctcaacgtt caggtagaat
    16381 tgataaagct acaactctaa atacttattc tcatttttac aatgctagaa agcagacagt
    16441 aggtaatgag ataacagaat tattgaaaaa ggagggtgtt tcatcaacac cccgataagg
    16501 accactatca ataatatttg actaaaaaat gcataatact aaggtttaaa agcgatttgc
    16561 ttatgttact cttaaataag aagcacatag caatttgaga gcggaagccc gatttaccag
    16621 tcttttagag cttatagcga tttaaagaaa ctgataattt gatttggttt cctgccaaaa
    16681 atcctgccaa aaaatctttg gcaggatttt tgtttttata taaatatact gtgcagaatt
    16741 gctattcatc aacatttgta tttgtagaaa tatattttta tgtgagcgag aacatttcaa
    16801 taagtgttct cgcttttttt attttcagga ggaaaatcat gcaaaagaat gaacagtcat
    16861 cacgacaaat tgtaatgcgt catctcatgg ctatcatgag aatcgatatc gagaaagcga
    16921 agcggatagt tgctgaaatg gaggagaaag gactcattca atttgatgag ttgggaaatg
    16981 ttggcctgtt agtgttggag gtacaatcat gaaacgtatt accgcaaatc actatcaaac
    17041 gtcagagcgt tattataagc tccctaaact cttatttgag agcgaacgct acaaggacat
    17101 gaaacttgag gttaatgttg cttatgctgt tctaaaagac cgtttggagc tatctttgag
    17161 tagaggatgg attgatgaag atggagctat ttatttggtc tattccaact ccaaactcat
    17221 ggcactactt ggctgttcca agtcaaaatt actgacgatt aagaaaacct tacgagagta
    17281 tggtttgatt gatgaagtcc aacagtcttc cagtgaaaaa ggacgcttgg ccaataagat
    17341 ttacttgggg gagttagaac atgaacctac cccagtctca aattcagacg ggggtagtgt
    17401 tcaaaaaaca ctagggaggt gtcaaaatca gccggggccg gtcttaaatt tagccactag
    17461 tgagactgaa gttagtgaga ctgatatgag tgaaactaaa gagagtgaat cagtcattga
    17521 ggacgaggag gagaaagaaa gtctaacaag taagaaaagt gacaatgaca attttcaacg
    17581 taaagtagac cgagtcacaa gatacgatag agattacatt tggggcttgg tgcatgacca
    17641 gttgagacaa gttggtctat cacgtgccgc tagtgattat gccatgcttt attttgatca
    17701 tcgttatcag tacgctttag agaatatgag gtttgcggac agaacagata cgatagcgga
    17761 atacgtcttt aatggtgtct tatccgaatg gactaaggta caacgcttaa aagaattaaa
    17821 agggggtgag taagatgttt tggtggatat tattaggtat ttggggatta ggaatgattt
    17881 ggttcatttt tgaacttatc actgccccag aaatggaaga tcattgttaa gcaaggacgt
    17941 tacgcaagaa gtaaggtcct ttttagtttt gtcagaaagg aatgagagac atgaagtttt
    18001 tagttttgtc agaaaggaat gagagacatg aagtttttag atttgtttgc aggaattgga
    18061 ggctttagac tggggttaac ccgtcagggc cataagtgta ttggcttttg tgagattgac
    18121 aagtttgcga ggaaatctta caaggccatc tatgagaccg aaggagaaat agaatttcat
    18181 gatattagac aagtcacaga ccaagacttt agacagctca gagggcaagt ggacatcatc
    18241 tgtgggggat tcccttgcca ggcattttca ctcgcaggca gacgattggg atttgaggat
    18301 actaggggaa ctttgttctt cgagattgct cgagcgacca aacagatcca accacgtttt
    18361 ttattcctcg aaaatgtcaa gggcttactc agtcacgaca agggagagac atttcgaaca
    18421 atcctcgcca cattggatga gttggggtat gatgttgaat ggcaggtgct taacagtaaa
    18481 gatttccaag tgccgcaaaa ccgtgaaaga atttttatta tcggacattc tagaagatac
    18541 cgtccccgat tcctatttcc tctcagagga gaaaacagct cagctggtct tgaacgatta
    18601 ggtaatgtta atccgtcaag caaagggatg aatggagaag tctatttatc acgtgggata
    18661 gcaccaacct taacaagagg aaaaggtgag ggaactaaaa ttgccattcc agttttaacc
    18721 cctgataggc tagaaaaacg ccaacatggg agacgcttta aagggaacga tgatccgatg
    18781 tttactttaa ccagccaaga tcggcatggt gtagttgtcg caggaacttt accaacttct
    18841 tttatccaaa ctggacgagt ctatgacctt tcagggcttt ctccaacgtt gacaaccatg
    18901 caaggagggg ataaagttcc taagattctt tgccgtgaag aagcgccaca tttgaaaatt
    18961 agggaagcga ccaaactagg ctatgccaag gcaatagtgg gagattctgt caaccttgct
    19021 tacccagagt ctaccaaacg cagaggtcga gtgggaaagg ggatttcaaa taccttaacc
    19081 acttctgaca atatgggagt ggtagttgct gctatggaat accgtaagga caagtggtat
    19141 gaagtgactg gaataattct agatggaaaa ctgtatcggt tgagaatcag acgcctaaca
    19201 cctagagagt gttttagact tcaggggttt cctgactggg cttttgacaa agcagagagc
    19261 gtatccagta aaagtcagtt gtataaacaa gctggcaata gtgtgaccgt cactgttatt
    19321 gaagccattg ctagagaatt tagaaagatt gaagaggaag aagaacatga aattattaca
    19381 tagtaaaagt attaggaact gcacggagct agaagaagcc atacatcaag cagaagtgga
    19441 gagattttcg gaaatgattg cttctttacc caattatgat tgtgacattg atgtcacttt
    19501 tgaagatgac taccacaaag aaatgaattc tccgttagct tatgagtcca acttacaccg
    19561 tatttttgag tttattgaaa cccaagacat caaaaatggt gttgatactt atctaacgga
    19621 tgagaacaat ctcgccttta gggcatatgg gcaaggctat tcttggaatg ataagaatga
    19681 tgtcgtcacc acattaataa cagtgaagtg ttttggtgaa ggcaggtcgc ctattgatat
    19741 gagtaaggtg tttacctcac caactcaagc attagagaag gagctatctg tgtaggagga
    19801 agcgatgtta gaaatctatt taagccgaaa taacagtcgg aatcaaaaac tcctgtcttt
    19861 ttgtgactcg catggtattt cctactcctg taaagaagtg agtcatctat cacgtgagga
    19921 attactgagg ttatttacta aaagcagtga ttgttttacg ctcttatccc cctcgctaca
    19981 acgatttaaa ggtcatagag agatgaaatt gagtgagtta gtgacgttgg tgttacgaaa
    20041 acctgaccag aatcttcgcc tcccacttgt ggtttgtgag gacaacgtgt atcccgatat
    20101 gagtttagaa gaagcaagga cctttcttcc gagaaatcaa aaagtagttt cctttcgtga
    20161 acaccttttt aaggacctgg tgacataagg aggagttatg aacgaccgtt tttgggaaaa
    20221 tttggaaatt atcgttatgg aaaaaggact gacatgggct gaccttgctc gacagatgtt
    20281 taagggacaa tacgtctacc ctagtgagtt taaccgtctc taccaaacct ttcgtcacta
    20341 caaatctcat cgtctcatgc cacaaggcaa atgggtggag aaaatagtga gtgttttaga
    20401 gattgactat gaagatttgt ttaggaggta gagatgaaac gtttagtagt catgtacgga
    20461 gccattcatg tcaatgtact cctagtgagt ttttacctgg ttggttggct aaatggggca
    20521 tggttacacg ttttacaagt gacattttta gccctgctat tgtggggttg gaaaaggttt
    20581 aagatacaga aaagaaactt atcctttaag gagagagggc tttgggtgtg tggtagtcta
    20641 ggtgtgatgg caagtatggc tcttatcatg agtggtctat tttcaggaag cgaaccaaac
    20701 caagaaacgt tagtgactgt tcaagaccag attcctgttc tgtcctttat tctttttctc
    20761 cttaatgcca gtgttgtgga agaagtcttt tatagagagg tgttatgggg agtcttgtct
    20821 caaccagtgg ttcaagtgtt gttaacaagt tccctttttg ctttagctca tcacccatcc
    20881 agtctattca cttgggtgct ttatgggagt ttaggtctca cacttggtgt ggtgagaggg
    20941 caaacagact gcttgacgtc cacacttatt cacctgtctt ggaacggaat tgtctttttc
    21001 ctatctttat tgtgatacca aaatcaaggg tgctttcgta tcatgaaagc accctattgt
    21061 tttgatgggg aatcatataa aggaggaaat gatgtcatcc gaacaacagg aacgccaagc
    21121 gattcaatat gcagaacgga gtgctatgtt tacggtgaag gtccttctca aactcttaga
    21181 gtggtcagcc agacacgccc tagcacaaga ctcggcttat aaagttgggg ttcaaaagtt
    21241 agaagaactc cttcaaagtc catatgccat tgagtcgctg aatatctcaa aagacatttt
    21301 ggataaaccc gttgatgtgg agaaattcaa ggaattggtg gaatcagaaa accttcctat
    21361 tgccattagt tggcaaggag attacctgca tttttatgct aaggataagt cgctcttaga
    21421 caagcactta aatgagttgc ttcagaagtt ggtttccaat ccagaaaaac tgaaagggct
    21481 aactctggat aagacgctcg atgaggaaat cgcacaagct aaggaacaga ttgtgatgtc
    21541 agaaccctct gctgtgaaat caaaggaggt cattctctaa tggtctcagg gaaaaaagtt
    21601 tttatttttg gtgtattagg acttgctctt ggctatttct gccatcggct agtcttgctt
    21661 tacgatagcc tacctaatca accgcccttg gaacgccttg cctatctctt aggcgaagga
    21721 cagaatcagg tcttaaatcc cttgtggaat ggtcatttta ctagtagatc ggttttaggc
    21781 ttttgctttg gatttgtgac gatgggccta gtctatcttt atgtttcaac tggtcaaaag
    21841 gtttaccgag agggggctga atatggctct gctcggtttg gaaacagtca cgaacgcaaa
    21901 gcctttatca gtaagaatcc ttttaacgat accatcttgt cacgaaatgt taggctaacc
    21961 ctgttagaaa agaaagcccc acagtttgac cgaaataaga accttgtggt tattggtggg
    22021 tctggtagcg ggaagacctt tcgctttgtc aaacccaatc tgattcagct caactgttcc
    22081 aatattgtcg ttgaccctaa ggatcactta gctgaaaaga cagggaagct ttttttagca
    22141 catggctatc agattaaggt cttagacctc gtaaacatga ccaactctga tggctttaac
    22201 ccttttcgtt atgtggagac ggagaatgat ttgaaccgta tgcttacggt ctatttcaat
    22261 aataccaaag gtaatggcag ccgcagtgat cccttttggg atgaagcgtc catgacctta
    22321 gtccgtgcga ttgcctctta cttggtggat ttttacaatc ctccaggcag taccaaagaa
    22381 gaagcagaca gtcgccgtaa gcgtgggcgt tacccctcat tttctgaaat tggaaaactc
    22441 atcaagctct tatccaaagg agaaaatcaa gataagagtg tcttagaagt gatgtttgag
    22501 acctatgcca agacatatgg gacagaaaac ttcactatgc gtaactgggc agacttccaa
    22561 aactacaagg ataagacctt ggattcagta attgccgtca caacagctaa gttttccctc
    22621 tttaatatcc aatccgtcat tgatttaacc aaacgtgaca ccttggactt aaaaacatgg
    22681 ggaacgcaga aaactatggt ctatctcgtt attccagata atgacaccac ctttcgcttt
    22741 ctctcagccc tctttttctc caccgtcttt tcaactttaa cgagaca
//
LOCUS       DATUCG010000021        21840 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.022, whole genome shotgun sequence.
ACCESSION   DATUCG010000021 DATUCG010000000
VERSION     DATUCG010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21840
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.022"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(34..1206)
                     /locus_tag="VCA20_000665"
     CDS             complement(34..1206)
                     /locus_tag="VCA20_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HEP1834519.1"
                     /translation="MIPFALKYIMVIITTMVISAIATPLVRFMSVRVGAVDNPNARRI
                     NKVPMPSAGGLAIFVAFTISVLWFLPGIVTRVNYHGTYLHYIWPLVLASSIIVLTGFI
                     DDVKELKPAPKMFGILAAATIIWIFTEFRFDSFKIPFGGPFLQFPVWLSFLLTVLWIV
                     SITNAVNLIDGLDGLVSGVSIISLTTMGIVSYFFLHDSNIFLTLTIFVLVAAIVGFLP
                     YNYNPAIIYLGDTGALFIGFMIGVLSLQGLKNSTAVAVVTPMIILGVPITDTVVAIIR
                     RKLSGQKIYEADKMHLHHRLLSLGLTHRGTVLVIYAISFLFSLTSLLLNVSSRIGGVL
                     LLVTMGLGIEILCELIGIFGINRTPLLNLLRFIGNSSYRQEKIAKYKKCCEEKKRK"
     gene            complement(1203..1925)
                     /gene="mecA"
                     /locus_tag="VCA20_000666"
     CDS             complement(1203..1925)
                     /gene="mecA"
                     /locus_tag="VCA20_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEP1834520.1"
                     /translation="MKVKQISDSTLKITIKLDDLEERGMELSDFLIPQEKTEEFFYTV
                     LDELDLPMTFRESGMLSFRVTPKPDRVDIFVTKSDLDQSLNFDEFTDLSELGDVASMT
                     PDEFFKSLEQTVREKSAPDATAVRHLEEVEQEEEADEEEQERYIYYILEFPTIEDLFT
                     FVGTVDYPVEESELYKMDGHYYLTVLINVENRSKQYPGYILSRMLEFTNDTKLTRPAL
                     QEHGTLMLPLAAIEELRKVPTV"
     gene            complement(2053..2889)
                     /locus_tag="VCA20_000667"
     CDS             complement(2053..2889)
                     /locus_tag="VCA20_000667"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEP1834521.1"
                     /translation="MLLELLKAIFLGIIEGVTEWLPVSSTGHLILVQEFVKLNQSKNF
                     LEMFNIVIQLGAILAVMTIYFKKLNPFQPGKTKRDIQLTWQLWAKVVIACIPSILIAV
                     PLDNWFEAHFNFIVPIAIALIVYGIAFIWIENRNRGIEPQVTDLAKMSYKTALLIGCF
                     QVLSIVPGTSRSGATILGAIILGTSRSVAADFTFFLGIPTMFGYSGLKAVKYFLDGNS
                     LNMEQVWILLVASVTAYLVSLVVIRFLTDFVKKHDFTVFGYYRIILGAILLVYAFITF
                     LF"
     gene            3016..4581
                     /locus_tag="VCA20_000668"
     CDS             3016..4581
                     /locus_tag="VCA20_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEP1834522.1"
                     /translation="MKRKLSIFLLTLIAVFSVTTGVQADEYLRVGMEAAYAPFNWTQE
                     DDSNGAVPIEGTNQFANGYDVQVAKKIAASMDKELLVVKTKWEGLVPALTSGKIDMII
                     AGMSPTEERKKEIAFSDSYYTSIPTLVVRSDSQYANATSLADFAGAKITAQQGVYLYD
                     LIDQIEGANKQTAMGDFSQIRQALESGIIDAYVSERPEGRTAEAANKAFKMVELADSF
                     ETNAEDVTIAVGMRKDDARIPQVNEVLATISEEEQIALMDDMIENQPVEEASEGETPS
                     FFAQVWNIVVNNWQQLLRGTGMTLLISILGTIIGTAIGLLIGVFRTAPKAANKALAIG
                     QKVLSWIINIYIEIFRGTPMIVQSMVIYYGTAQAFGLNLDRTLAAIFIVSINTGAYMS
                     EIVRGGIFAVDKGQFEAATALGFTHNQTMRKIVLPQVIRNILPATGNEFVINIKDTSV
                     LNVISVVELYFSGNTVATQTYQYFQTFTVIAIIYFILTFTVTRILRAVEKHFDTDTYT
                     TGANQMQVEVPHD"
     gene            4574..5317
                     /locus_tag="VCA20_000669"
     CDS             4574..5317
                     /locus_tag="VCA20_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEP1834523.1"
                     /translation="MTKNVILEIKNLKKSYGQNQVLKDISISVEKGEVISIIGSSGSG
                     KSTFLRSINLLETPTEGQILYHGQDVLAKGYGLTTYREKLGMVFQSFNLFNNLNVLEN
                     AIVAQTTVLKRDRAEAEKIAKENLNKVGMTEQYWQAKPSQLSGGQKQRVAIARALSVD
                     PEVILFDEPTSALDPEMVGEVLKTMQDLAKSGLTMLIVTHEMEFARDVSDRIIFMDKG
                     VIAEEGSPQQIFENPQEERTREFLQRFLG"
     gene            complement(5445..5654)
                     /locus_tag="VCA20_000670"
     CDS             complement(5445..5654)
                     /locus_tag="VCA20_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834524.1"
                     /translation="MTSYEKVAAVVGWVREKKITGYRISKETNAREMSVIALAQGRAK
                     IDNISFATALGLIDFYDKNHKKFEN"
     gene            complement(5740..6642)
                     /locus_tag="VCA20_000671"
     CDS             complement(5740..6642)
                     /locus_tag="VCA20_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEP1834525.1"
                     /translation="MVLGPIVFIGSFLFFVLIALILIASGLYVVKQQTVAIIERFGKY
                     QKTSTSGINFKIPFGVDVIAARIQLRMLQSEIVVETKTQDNVFVTMNVATQYRVNENN
                     VTDAYYKLMHPEAQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQKQVAEEMSTYG
                     YVIVKTLITKVEPDAEVKQSMNEINAAQRKRVAAQELAEADKIKIVTAAEAEAEKDRL
                     HGVGIAQQRKAIVDGLADSIRELKESNVSLSEEQIMSILLTNQYLDTLNNFAQGGNQT
                     IFLPGNPEGVEDIRTQILSSLRVK"
     gene            complement(6652..7902)
                     /gene="ilvA"
                     /locus_tag="VCA20_000672"
     CDS             complement(6652..7902)
                     /gene="ilvA"
                     /locus_tag="VCA20_000672"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019322143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HEP1834526.1"
                     /translation="MITAKNVEQAYSVLKRVVVRTPLDYSRYLSEKYGATIYLKRENE
                     QRVRSFKIRGAYYAISQLTDDEKSRGVVCASAGNHAQGVAYTCQEMQIPATIFMPITT
                     PQQKIGQVKFFGGDYVEIRLVGDTFDESAKAAQDYTQESGKTFIDPFDDENVQAGQGT
                     VAYEILEEAEEQTISFDQILVPIGGGGLVAGVSVYLKEHAPEIKIVGVEASGARSMKA
                     AFDKGRPVKLDQIDKFADGIAVQKVGKSTYEVARKYVDRLIGVDEGWISWTILDLYSK
                     LGIVAEPAGAATIAALEVVKDQIKGQTICCIISGGNNDINRMPEMEERALIYEGIKHY
                     FVVNFPQRPGALREFVNDILGPNDDITRFEYIKRANKGTGPVLIGIALGDKKDYAQFI
                     SRLEEFDPQYINLHENDSLYKMLV"
     gene            complement(8184..9206)
                     /gene="ilvC"
                     /locus_tag="VCA20_000673"
     CDS             complement(8184..9206)
                     /gene="ilvC"
                     /locus_tag="VCA20_000673"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEP1834527.1"
                     /translation="MTVTMQYEKDVTVAALDGKRIAVIGYGSQGHAHAQNLRDTGHDV
                     IIGVRAGKSFDKAKEDGFETFEVAEAAKQADVIMILAPDEIQADLYNDEIAPNLEAGN
                     ALGFAHGFNVHFEFIKVPADVDVFMCAPKGPGHLVRRTFEEGFGVPALYAVYQDATGN
                     AKHIAMDWAKGVGSARVGLLETTFKEETEEDLFGEQAVLCGGLTALMQAGFEVLTEAG
                     YAPELAYFEVLHEMKLIVDLVYEGGFKKMRQSISNTAEFGDYVSGPRVITDQVKENMK
                     AVLADIQSGKFANDFVNDYKAGRPRMEAYRKEAENLEIEKVGAELRKAMPFVGRNDDD
                     AFKIYN"
     gene            complement(9338..9814)
                     /gene="ilvN"
                     /locus_tag="VCA20_000674"
     CDS             complement(9338..9814)
                     /gene="ilvN"
                     /locus_tag="VCA20_000674"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEP1834528.1"
                     /translation="MRRMLTAKLRNSSGVLNRFTGVLSRRQINIESISVGPTEVDGIS
                     RVTVIVDVVSHDEVEQIIKQLNRLIDVVRVRDLTDIPHLEREVILVKISAPPTKRAEI
                     LAIIQPFRANVVDVAPHSITIQMTGDGDKIDALLRVIQPYGIKNIARTGATGFSRD"
     gene            complement(9807..11510)
                     /locus_tag="VCA20_000675"
     CDS             complement(9807..11510)
                     /locus_tag="VCA20_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEP1834529.1"
                     /translation="MQEIQLDQPRSGSYLILDTLHQLGVDLVFGYPGGAVLPLYDAIY
                     QYEGIQHILARHEQGAVHEAEGYAKSSGKVGVAIVTSGPGATNAITGIADAMGDSVPL
                     LVFTGQVATRGIGKDAFQEADVLGMTMPITKYNYQIRDTADIPRVITEALHIATTGRP
                     GPVVIDVPKDIQERVVDFYHDPTLHLPSYQPTIEPNGLQVKKILKQLSQAQKPVILAG
                     GGVNYADANKELVAFAERYRIPVVSTLLGLGAMPIEHELSLAMGGMHGSYAANMAMDQ
                     ADCIINIGARFDDRLTGNPTTYAPNATIAHIDIDPAEIGKVVKTAIPVVGDAKATLKA
                     LLALETVETGYAEWTAQVLENKRRAPFWYDEDKKVIKPQAAIELIGQLTQGDAIVVTD
                     VGQHQMWAAQFYPYKHARQLVTSGGMGTMGFGIPAAIGAKLANPDKEVIIFVGDGGFQ
                     MTNQELAILNGYGVPIKVVLINNHSLGMVRQWQESFYEKRRSQSVFDDEPNFQLLAEA
                     YGISHYSFDNPATLSEDMKVILEDKPMLIEVHISNREHVQPMVPVGKSNAEMLGVKFN
                     A"
     gene            11735..12787
                     /locus_tag="VCA20_000676"
     CDS             11735..12787
                     /locus_tag="VCA20_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyltransferase family 2/GtrA
                     family protein"
                     /protein_id="HEP1834530.1"
                     /translation="MYYVVIPAYQPDEKLIQLLEIMKNRLNCQVIVVDDGSTGASKNI
                     LEIAKTYAIVLHHDVNKGKGQALRTAFSFIQDLRKSGVIVTADVDGQHAVNDIDRVAR
                     AAMNLPSRLILGVRQFTKDIPFRSRFGNKLTRVLFRLQTGVNVSDTQTGLRGFHTDLI
                     PFMLDIEGDRYEYEMNMLTQASQRYKITEVPIQTIYIDDNASSHFRPIKDGLLIYKNL
                     FKFALASFGGFLVDYGVYALALLLLTSLPTALRLLVANTIARICSATCNFVLNKKLVF
                     NNEESIARTGAGYFTLALVLFLCDTALLYLFHQILGLNLYIVKLAVGIFLFLASWFVQ
                     KNIIFKERKSFSHEIA"
     gene            12774..13709
                     /locus_tag="VCA20_000677"
     CDS             12774..13709
                     /locus_tag="VCA20_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002918105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HEP1834531.1"
                     /translation="MKLLKKPFAYASIFGMLLTGGFTYSMLKTFVLSEAITTVAASNA
                     TANSQNTATSSSSTAATTAANVSTTDTSYSDDNIQINLETITTNNTIVYVADIQVSSP
                     EYLKTALAQNTYGTNVTAKTSETAAANNAILAVNGDYYGANSTGYVIKNGVLYRDTVR
                     DNAAYGDLAIYADGSFEVIYENEITAQELIDKGVVNLLAFGPSLVENGEIVVDTSTEV
                     GRAMSSNPRSAIGIIDENHYIIVVADGRTSESQGLSLYQMAEVMKQYGAQTAYNLDGG
                     GSSTLYFNGQVINNPTTNGNIISERAVSDIVYIGY"
     gene            13684..14100
                     /locus_tag="VCA20_000678"
     CDS             13684..14100
                     /locus_tag="VCA20_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834532.1"
                     /translation="MTLSTSVTNSTTRVSRTFISYLAITAFLFIFSRIYESLSYGEVS
                     VFMHYMFCVPLIGGSVLLGLLQLIPTLSRLSYNLWNSGVATVAAGCLLRGIINLSGRS
                     TTLDQPYWYIGAAFLVLSLVGLFINPSANNRRKTTT"
     gene            14261..15976
                     /gene="ilvD"
                     /locus_tag="VCA20_000679"
     CDS             14261..15976
                     /gene="ilvD"
                     /locus_tag="VCA20_000679"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEP1834533.1"
                     /translation="MTDTNKLKDFRHRSSVYDSMVKSPNRAMLRATGMTDDSFEKPIV
                     GVISTWAENTPCNIHLHDFGKLAKEGVKDAGAWPVQYGTITVADGIAMGTPGMRFSLT
                     SRDIIADSIEAAMGGHNVDAFVAIGGCDKNMPGSMIAIANMDIPAVFAYGGTIAPGNL
                     NGKDIDLVSVFEGIGKWNNGDLTAEEVRQIECNACPGPGGCGGMYTANTMATVIEVMG
                     MSIPGSSSHPAESPEKKADIEEAGRAVVRMLELGIKPSDIMTREAFEDAITVTMALGG
                     STNATLHLLAIAHAANVELTLEDFNDFQERVPHLADLKPSGKYVFQDLYNVGGVPAVM
                     KYLLKNGFLHGDRITCTGKTVAENLEAFADLTPGQDVIMPLENPKRADGPLIILKGNL
                     APEGAVAKVSGVKVRNHTGPAKVFDSEEEAIEAVLTDEIVDGDVVVVRYVGPKGGPGM
                     PEMLSLSSMIVGKGQGDKVALLTDGRFSGGTYGLVVGHIAPEAQDGGPIAYLRTGDLV
                     TVDQDTKEITMHVSDQEIEERKKTTVIPPLYSRGVLGKYAHTVSSASKGAVTDFWRPE
                     RTGKK"
     gene            16027..16413
                     /locus_tag="VCA20_000680"
     CDS             16027..16413
                     /locus_tag="VCA20_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834534.1"
                     /translation="MKLNVELSRFRVKEGKTVQVDEWMAFLNEHMEDTLLTLEGEKMY
                     VETIFREVLDGREYLYWYSVQAEGGIEVEDSESYIDKKHLEYWEECIDPSYGIVDLDP
                     QVIMIPKPVYETMEELNRQYDETFKK"
     gene            complement(16440..16781)
                     /locus_tag="VCA20_000681"
     CDS             complement(16440..16781)
                     /locus_tag="VCA20_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF59 domain-containing protein"
                     /protein_id="HEP1834535.1"
                     /translation="MREDIQINERALVLSDQLVEVLESIYDPEIELDIYNLGLIYEIH
                     LDEAGFCKVVMTFTDSGCSCADTMPGELVAALKTIDGINDAQVEIVWSPAWKMTRISR
                     LGRITLGISPK"
     gene            complement(16872..17321)
                     /locus_tag="VCA20_000682"
     CDS             complement(16872..17321)
                     /locus_tag="VCA20_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEP1834536.1"
                     /translation="MELKDIMHILEDMKVGVFATLDEYGNPHARHAHITAANEEGIFF
                     MTSPETHFYDQLIGDQRVAMTAISEEGYLIQVVRVEGTARPVENDYLKTVFADNPYYQ
                     HIYKDESSDTMQVFQIYAGHGFYHSLTQGHKYIFSIGQGEHSEVRAL"
     gene            complement(18145..18435)
                     /locus_tag="VCA20_000683"
     CDS             complement(18145..18435)
                     /locus_tag="VCA20_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002918303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834537.1"
                     /translation="MNRKAIYEDMIQFDYFSDSYLQFEKDFNRYYKGVDPLTFLIDDI
                     LLTMAMKQKNYFRLHKSKSTDGRDHYFIFKVRTTNENKRIRIYEYAETKTMI"
     gene            complement(18428..18787)
                     /locus_tag="VCA20_000684"
     CDS             complement(18428..18787)
                     /locus_tag="VCA20_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834538.1"
                     /translation="MIDRSYLPYQSARDYQDRGMQKWMGFFLSEHTSSLMDDKIRIDM
                     SSKLSKIEILTYITQLYSSQSQGMFIVVRNGKREAYQGKITELSVKEITIKTIQKYEL
                     IQITEIIEISLVEVLDE"
     gene            complement(18784..20193)
                     /locus_tag="VCA20_000685"
     CDS             complement(18784..20193)
                     /locus_tag="VCA20_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002918301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HEP1834539.1"
                     /translation="MIDYSREPESDIAFIDMKSFYASVECVSRGLHPLKTSLCVMSRA
                     DNSGGLILASSPVFKEVFGKSNVGRAYDLPFDINTRKFSYYNAKRQGLPTDPAYVKFI
                     EEWAVATVIVPPRMDLYIEKNINIQHIFQQYGSIEDIMPYSIDEGFIDLTSSLNYFIP
                     NQTISRKDKLDMLSARIQRDIYRKTGVYSTIGMSNANPLLAKLALDNEAKKTHNMRAN
                     WSYEDVTTKVWGISKLTDFWGIGTAIQKRLEKLGIYTIKELAKYNPDLLKKEFGIYGV
                     QLWFHANGIDESNVHIPYKPKSKGLGNSQVLPRDYFKQYEIELVLKEMAEQVAIRLRG
                     IHKKTTTVYIGIGYSWFEEKTSFRGQMKIEPTNLTEDLQKYVISLFRKKYNGGAVRSI
                     AVSYSSFVDENLTILNLFDDPEELLKKERLQTAIDSIRNQFGFTMLQKANVLTNASRS
                     IARSKLIGGHSAGGLDGLK"
     gene            complement(20186..20536)
                     /locus_tag="VCA20_000686"
     CDS             complement(20186..20536)
                     /locus_tag="VCA20_000686"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834540.1"
                     /translation="MKTKVIFPLGSENKHLAIENQRIVFNNYSEKIDVTLTVLSACQK
                     WFEENNFHITKSTIAEKNQALIFTNEREIIDYIEDFYDIKSADFSSVESDAEKEWLKS
                     LLEKNNITQCKEYD"
     gene            complement(20537..21238)
                     /locus_tag="VCA20_000687"
     CDS             complement(20537..21238)
                     /locus_tag="VCA20_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP1834541.1"
                     /translation="MFSGERLKQLRKESGLTQQDIAQKLGINRASYTSWELGRAKPNR
                     ANLKRLADLFGVEVTDFEHGYMILSNYLKLNQKNKNTAEQVVEELLKNQIEEEVANKV
                     IPLYPIQVLEEVPLSAGYGESYYNEYSFQTVYSDKEYNYDFASFISGESMEPKYFDGE
                     VALFSDSGFDYDGAVYAISLNGKAYIKRLFKEKDRYRIVSINPKYADMYAYEGDEFKI
                     VGKIVGHFIPIEEVQ"
     gene            complement(21335..21739)
                     /locus_tag="VCA20_000688"
     CDS             complement(21335..21739)
                     /locus_tag="VCA20_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834542.1"
                     /translation="MNIKKLILTLLTLTLTIVLCACGNQSNSNDSQLSGTYSYEKGGI
                     DGSEMGFEDEELTLHYELKVSGDENILNINLLSERGNNVKYLYSEKVTIDTDKQIISD
                     SNGTELKYSVSGDSVTIPDLAGDSGETVTLNK"
BASE COUNT         6496 a         4792 c         3955 g         6597 t
ORIGIN      
        1 gcctgacggc tcttatcttg tttagaaaga ttcttatttt ctttttttct cttcacagca
       61 ctttttatac tttgcgattt tttcttgacg gtaactacta ttaccaataa accgaagaag
      121 attgagcaac ggagttctat tgataccaaa gatgccaatc aattcacaga gaatctcaat
      181 tcccagtccc atggtcacca ataggagaac ccctcctatt ctactcgaaa cattgagcaa
      241 aagagaggtc agggagaata agaaagagat ggcgtaaatc accaatactg tgccacgatg
      301 agtaagtccc agagagagca agcgatgatg cagatgcatc ttatccgctt catagatttt
      361 ttgcccagac aattttcgac gaatgatggc tactacagta tccgtaatag gcactccaag
      421 aataatcatc ggagtaacca ctgcaacagc tgtcgaattt ttcagtcctt gaagggacaa
      481 gactccaatc ataaacccga tgaagagggc acctgtatcg ccaagataaa tgatggcggg
      541 attatagtta taaggtaaaa aaccaacaat cgccgcaacc aaaacaaaga tggttaaggt
      601 caaaaaaata ttgctatcat gcaaaaagaa ataagacaca attcccatcg ttgtcaagga
      661 aataatcgat actcccgaaa ccagaccatc cagaccatca atcaaattga ccgcattcgt
      721 aatggaaaca atccaaagga ccgttaatag aaaagagagc cagacaggaa attgaaggaa
      781 aggtcctcca aaaggaatct taaagctatc aaaacgaaat tccgtaaaaa tccaaatgat
      841 cgtcgctgca gctagaatac caaacatttt aggtgcaggc tttagctcct tgacatcatc
      901 aatgaatccc gtaagaacga taatgctgga tgctaggacc aggggccaga tataatgcaa
      961 gtaagtgcca tggtaattga cacgggtaac aattcctggt aaaaaccata ggacagaaat
     1021 ggtaaatgcc acaaagatag ccagacctcc cgctgaaggc atgggcacct tatttattcg
     1081 ccgtgcattg ggattatcaa ctgctccaac cctcacggac ataaaacgaa ccaagggggt
     1141 cgcaatggct gatatgacca tggttgtaat aatgaccatt atgtatttta gagcaaaagg
     1201 aatcatactg tcggaacctt cctaagctcc tcaattgccg caagcggaag catcaaagtt
     1261 ccgtgttctt gtaaagcggg tctggtcaac ttagtatcat tggtaaattc caacattctt
     1321 gagagaatgt agcctggata ctgcttggaa cgattttcta cattgataag aactgtcaag
     1381 tagtagtgac catccatttt atataattcc gattcttcta ctggataatc aaccgtacca
     1441 acaaaagtaa acagatcctc tatcgttgga aattccaaaa tatagtaaat atagcgctcc
     1501 tgctcctctt catctgcttc ttcctcttgc tctacctctt ccaaatgacg aacggctgtt
     1561 gcatccggtg cgcttttctc acgaacggtt tgctccaaac tcttgaaaaa ctcatctgga
     1621 gtcatacttg cgacatcacc aagttccgac aaatctgtaa attcatcgaa attcaagctc
     1681 tgatccaaat cagatttggt aacaaagatg tccacccgat ctggcttcgg agtcacacga
     1741 aaactcaaca taccgctttc tcggaaagtc attggtaaat ccaattcatc caaaacagta
     1801 tagaagaact cctctgtctt ttcttgcgga atgagaaagt ccgaaagttc cattcctctc
     1861 tcttccaaat catctaattt tatagtgatt ttcaaggttg aatcactaat ttgctttact
     1921 ttcataacta cctcattcat tgattcatca atttcttaaa attgattgat cattttcaat
     1981 actcatacat atcttccacc attatactaa aaccaagctc gaaagacaaa agaaaagcag
     2041 gtctccctgc tcttaaaata aaaatgtgat aaaggcataa accaataaaa tcgcccctaa
     2101 aatgatgcgg tagtaaccaa agaccgtgaa atcatgtttt ttaacaaagt ctgtcaagaa
     2161 acgaataaca actaaggata ccaagtaagc cgtcacacta gcgactagca agatccatac
     2221 ctgctccata ttcaggctat tgccatctaa gaaatattta acagccttca aaccactata
     2281 accaaacatg gttggaatcc caaggaagaa ggtaaaatct gccgccacag atcggctggt
     2341 ccccaaaata atcgctccca gaatggtcgc acctgagcga ctagtccccg gaacgatgct
     2401 taagacctgg aaacacccaa tcaacaaggc agttttataa gacattttcg ctaagtccgt
     2461 cacctgtggc tctatacctc tgttacggtt ctcaatccaa ataaaggcaa taccgtagac
     2521 aatcaaagca atcgcaatcg gtactataaa attgaagtgc gcttcaaacc aattatccaa
     2581 cgggactgca attaggatgg acggaatgca ggcaatgaca accttggccc ataattgcca
     2641 agtcaattga atatcacgct tggtttttcc aggctgaaat ggattcaatt ttttaaagta
     2701 aatagtcata accgctagaa ttgctcctag ctgaatgaca atattaaaca tttctaggaa
     2761 gttcttactt tggtttaatt tcacgaactc ttgaaccaaa attaaatgcc ccgttgaaga
     2821 aactggcaac cattccgtca ccccttcaat gattcctaaa aatatagcct tcaacaattc
     2881 caatagcata aaaagctcct tttcatcaat tctctacatt ataccataaa aaaactccct
     2941 attcatagta caaggatatt gaaatttgtc agaattttta ataaaatggt gtaatagcat
     3001 tttaaaggag acattatgaa acgtaaactc agtatatttc tgctgaccct aatcgctgtt
     3061 ttttctgtaa caacaggggt tcaggcagac gaatacctgc gggtcggtat ggaagctgcc
     3121 tatgcgccat ttaactggac ccaagaagat gatagcaacg gtgccgttcc tattgaggga
     3181 accaatcaat ttgccaacgg ctatgatgtt caggttgcaa aaaaaatcgc tgcttccatg
     3241 gacaaagaac tcttggtcgt taaaaccaag tgggaaggct tggtccctgc tcttacatct
     3301 ggtaaaatcg atatgattat cgccggtatg agcccgacag aagagcgcaa gaaagaaatc
     3361 gccttctcag atagctacta cacttcaatt cctaccctgg ttgttcgttc tgatagtcag
     3421 tacgcaaacg caaccagctt ggcagatttt gctggcgcaa aaatcactgc ccagcaaggg
     3481 gtttacctct atgatttgat tgaccaaatt gaaggggcta acaaacagac tgccatggga
     3541 gatttctccc aaatccgtca agctttagaa tctggaatta tagacgccta tgtttctgaa
     3601 cgtccggaag gtcgtacagc agaagctgca aataaagctt tcaaaatggt tgaattggca
     3661 gacagttttg aaaccaatgc cgaagacgtg accattgctg tcggtatgcg caaagatgac
     3721 gctcgtatcc ctcaggtcaa cgaagtctta gcaactattt cagaagaaga gcaaatcgca
     3781 cttatggatg atatgattga aaaccagcct gttgaagaag ccagcgaggg cgaaacacct
     3841 agtttcttcg cccaagtttg gaatattgtt gttaacaact ggcaacaact tctgcgcgga
     3901 actgggatga cattgctcat ttccatcctc ggtacaatta tcggtactgc tatcggtctc
     3961 ctcatcggtg tcttccgtac agctccaaaa gcagcaaaca aggcgcttgc tattggtcaa
     4021 aaagtgctca gttggattat caatatctac atcgaaattt tccgtggtac gccgatgatt
     4081 gtacaatcca tggttattta ctatggtacc gcccaagcat tcggactcaa tcttgaccgc
     4141 acacttgccg ctatcttcat tgtatccatc aacacaggtg cctacatgag tgagattgtc
     4201 cgcggtggta tctttgccgt cgataaaggc caatttgaag ctgctactgc tctcggattt
     4261 acccataatc agaccatgcg taagattgtc cttccacagg ttattcgtaa catcttacca
     4321 gcgactggta atgaatttgt catcaatatc aaagatactt ctgtattgaa cgttatctca
     4381 gttgttgaac tctatttctc aggaaatact gtcgcaaccc aaacctatca atatttccaa
     4441 acctttacgg ttattgctat tatctacttc atcctaacct ttactgtgac acgtatctta
     4501 cgcgcagttg aaaagcattt tgatacagat acatacacaa caggtgcaaa ccaaatgcaa
     4561 gtcgaggtgc cacatgacta aaaatgtaat tcttgaaatc aaaaatctaa agaaatcata
     4621 cggacaaaat caagtcctca aagacatctc tattagcgtt gaaaagggcg aggttatctc
     4681 aatcatcggt tcatctggtt cgggaaaatc aaccttcctc cgatctatca accttttgga
     4741 aacaccaaca gaaggacaga ttctatacca cggacaagat gttttggcta aaggatatgg
     4801 cttgacgact taccgtgaaa aactaggtat ggtattccaa tccttcaacc ttttcaacaa
     4861 tctaaatgta ttggaaaatg ccattgtagc acaaactacg gtactgaaac gagatcgagc
     4921 tgaggcagag aaaatcgcca aggaaaacct caacaaggtt ggcatgaccg aacagtactg
     4981 gcaagccaaa ccaagtcaac tctctggtgg acagaaacaa cgcgtggcca ttgcacgcgc
     5041 cctatctgtt gacccagaag ttatcctctt tgatgagccg acttctgctc tcgatccaga
     5101 gatggttggg gaagttctca aaaccatgca agatttggca aaatcaggat tgacgatgct
     5161 tatcgtaact cacgaaatgg aatttgcccg tgacgtatct gaccgcatta tctttatgga
     5221 taagggtgtc atcgccgaag aaggcagccc tcaacaaatc tttgaaaatc cacaagaaga
     5281 acgcacacgt gaattccttc agcgttttct tggttaaact caatctattc catagaagac
     5341 tattacttac cagtttcaaa gctggtaagt ttttttagac taaaaaaagg ggaattcgta
     5401 gaattcccct cctttttgga catctctaaa tgaaaccatt tagattagtt ttcaaatttc
     5461 ttgtggtttt tgtcgtaaaa atcaatcaga cccaaggcag ttgcaaatga aatattgtca
     5521 atcttagcgc gaccttgtgc aagagcgata actgacattt cacgagcatt tgtttcctta
     5581 ctgatacggt atccagtgat tttcttttca cgaacccagc caacaactgc ggcaactttt
     5641 tcgtaactag tcatgagaaa cccctttcct ttttattcgt gcaattaagt ataatacagt
     5701 taaacctatt ttgtcaagta aattgttcgg tttttaccat cattttactc taagagatga
     5761 tagtatttgc gtccttatgt cttcaacccc ctccggattc cccggaagga agatagtttg
     5821 attacctcct tgtgcaaaat tattgagagt atccaagtat tggttggtca gcaagataga
     5881 cataatctgc tcttcagata ggctaacatt tgattctttc agttcacgaa tcgaatctgc
     5941 cagtccgtct acaatcgcct tacgttgttg tgcaatacct acaccatgca agcgatcctt
     6001 ttcagcctct gcttctgctg ctgtgacaat tttaatcttg tcagcttctg ccaattcctg
     6061 ggccgcaacc cgtttccgct gagctgcgtt aatttcattc atagattgtt taacctcggc
     6121 gtctggctct accttggtaa ttaaggtttt gacaataaca taaccgtagg ttgacatttc
     6181 ttctgctact tgtttttgta cctcaagtgc aatctcatcc tttttctcga agagctcatc
     6241 cagggtcaat ttaggcacag atgaacgcag ggcatcttca atataggact taatctgtgc
     6301 ttctgggtgc atgagcttgt aataggcatc cgtcacatta ttttcattta ctcgatattg
     6361 agttgccaca ttcattgtga caaaaacgtt atcctgcgtc ttggtttcta ccacaatttc
     6421 actttgcaac atccgcaatt gaatgcgggc tgcgatgaca tcaactccaa atggtatttt
     6481 aaagttaata cctgaagtag atgttttttg gtatttccca aagcgctcaa taatagcgac
     6541 cgtctgctgc ttgacaacgt atagccctga agcaatcaaa atcaaagcaa tcagtacaaa
     6601 gaacaagaaa ctaccaataa aaacaattgg accaagcacc ataatgacct cctaaactag
     6661 cattttataa agcgaatcat tttcatgaag attgatgtac tggggatcaa attcttccaa
     6721 gcgagaaata aactgcgcat aatctttttt atctcctagg gcaataccga ttagaaccgg
     6781 tcctgttccc ttattggcac gtttgatata ttcaaaacga gtaatatcat catttggccc
     6841 tagaatatca tttacaaatt cacgaagagc ccctggacgt tgtggaaaat taacaacaaa
     6901 gtagtgttta attccctcgt agataagcgc acgttcctcc atttccggca tcctgttgat
     6961 gtcgttatta ccaccagaaa tgatacaaca aatggtttgc cccttgattt ggtccttgac
     7021 tacctcaaga gctgcaatcg ttgctgctcc tgctggctca gcaacaatac ccagcttaga
     7081 atagaggtct aaaatagtcc aggaaatcca tccttcatcc acaccaatca ggcgatctac
     7141 gtatttccga gccacttcgt aggtcgactt accgactttc tgtaccgcaa taccgtcagc
     7201 aaatttatca atttggtcta atttaaccgg acgaccttta tcaaaagccg ctttcattga
     7261 ccgtgcccca cttgcttcaa caccaacaat cttaatttca ggtgcatgtt ctttcagata
     7321 gaccgaaacg cctgcaacca gaccacctcc tcctatggga actaaaattt ggtcaaaact
     7381 aatggtttgc tcttccgctt cttccaagat ttcataagct actgttccct gaccagcctg
     7441 cacattttca tcatcgaatg ggtctataaa tgttttccca ctttcctgag tataatcttg
     7501 tgctgccttg gctgactcat cgaaggtatc acctacgagc cgaatctcta cgtagtctcc
     7561 tccgaaaaat ttaacctgcc ctattttttg ttgcggagtt gtaatgggca taaaaatcgt
     7621 agcaggaatt tgcatttcct gacaggtgta ggccacacct tgagcatggt tcccggccga
     7681 agcacagacc actccgcgtg acttctcatc atctgtcaat tgtgaaatag cataataggc
     7741 tcctcgaatt ttaaaagagc gaacacgctg ttcattttct ctttttaaat aaatagttgc
     7801 accatatttt tccgacaagt atcgactata atccaagggg gtacgtacta ccacgcgctt
     7861 aagaacggag tatgcttgtt ctacattttt tgctgtaatc atagttccat taatttcttt
     7921 ctatatttat tgtatcatta ttccctagaa aatcctagta tttaaactat tattatgtca
     7981 ttttataaaa accgaacttt tttatttttt taatgtattc atgaatgaaa tgggcaaaag
     8041 agctggaaaa accagctctt ttattcattt tcagagatag gggaaaaaag ggaatgacat
     8101 cccctcatcc gccaactcaa accttaaatg tattgccatc actcaaagta aagctgacta
     8161 tcccttatct accatgataa tgtttaatta tagattttga atgcgtcgtc atcattgcgt
     8221 ccgacaaatg gcattgcttt gcggagttct gcacctactt tttcgatttc aagattctct
     8281 gcttctttac gataagcttc catacgtgga cggcctgctt tatagtcatt tacaaagtca
     8341 tttgcaaatt taccagattg aatatctgca aggactgcct tcatgttttc tttgacttgg
     8401 tctgtaatca cgcgtggacc tgatacatag tcaccaaatt cagcagtgtt tgagatagat
     8461 tggcgcattt tcttaaagcc accttcgtag acaaggtcaa cgatgagttt catctcgtgg
     8521 agaacttcaa aataagccaa ctctggtgca taacctgctt ctgtcaagac ttcaaaacct
     8581 gcctgcataa gggctgtcaa accaccgcag agaacagctt gctcaccaaa cagatcttct
     8641 tctgtctctt ctttgaaagt tgtttccaaa agaccaacac gggctgaacc aacacctttt
     8701 gcccagtcca tcgcaatatg ttttgcattg ccagttgcat cttggtaaac agcatagagg
     8761 gctggtactc caaaaccttc ttcaaaggta cggcggacca agtgacctgg accttttggt
     8821 gcacacatga agacatccac atctgctggt accttgataa attcaaagtg aacgttgaaa
     8881 ccatgggcaa aaccaagggc attgcccgct tccaagtttg gagcgatttc gtcattgtaa
     8941 aggtctgctt ggatttcgtc tggagccaag atcatgatga catctgcttg ttttgctgct
     9001 tctgctactt caaaagtttc aaaaccgtct tcttttgcct tgtcaaatga cttacctgca
     9061 cgcacaccga tgatgacatc gtgtcctgta tcacgcaagt tttgagcatg ggcatgacct
     9121 tgtgaaccat aaccgataac ggcgatacgt ttaccgtcaa gtgctgctac tgttacatct
     9181 ttttcatatt gcattgttac tgtcataatt ttttctcttt tctattttta tttgtagttt
     9241 tggatgttca aagaaaatga aggctagacc aggcgaagat agaactaacc ttcatcaagc
     9301 caagtcaacg caggataggt tttagtatcg aaaaggatta gtctctacta aagccagtcg
     9361 cacccgttct tgcgatgttc ttgatgccat aaggctgaat cacacgcagg agggcgtcaa
     9421 tcttatctcc atcacctgtc atttgaatgg taatggagtg cggtgccaca tcaacaacat
     9481 ttgctctaaa gggttggata atcgctaaaa tttctgctcg ttttgttggc ggagctgaaa
     9541 ttttaacaag aatcacctca cgctctaagt ggggaatgtc cgtcaaatca cggacacgga
     9601 ccacatcaat caagcggttc agctgtttga tgatttgttc gacctcatcg tgactgacca
     9661 cgtctacaat caccgtcaca cgtgagatgc catctacttc tgttgggccg acggagatgg
     9721 actcaatatt gatttggcga cgagatagga cacctgtaaa acgattcaga acaccagatg
     9781 aatttcgcaa tttagctgtt aacattctac gcattgaact tcacccccaa catttctgca
     9841 ttgctcttac ctactggcac catcggttga acatgttcac gattagaaat atgaacctct
     9901 atgagcattg gtttgtcttc taagataact ttcatatctt cacttagagt cgctggattg
     9961 tcaaaactat aatgactaat tccgtaggcc tctgccaaca attggaaatt cggttcatcg
    10021 tcaaagacag attggctacg acgcttttca tagaaggatt cctgccattg acgaaccatt
    10081 ccaagagagt ggttattgat caatacaacc ttaatcggta caccatagcc attcaaaata
    10141 gccaattctt ggttggtcat ttggaagccg ccatcaccaa caaagatgat tacttcttta
    10201 tctggattgg ctaatttggc gccgatagct gcaggaatac caaagcccat ggttcccatg
    10261 ccaccagatg ttacaagttg gcgtgcatgt ttgtaggggt agaattgggc agcccacatt
    10321 tgatgctgac caacatctgt cacgacaatg gcatcccctt gcgtcaactg accaattaat
    10381 tcaattgccg cctgtggctt aataaccttt ttatcttcat cataccaaaa tggcgcccga
    10441 cgtttgtttt ccaatacttg tgccgtccat tcagcatagc ctgtttcaac tgtttctaaa
    10501 gcaagcagag ctttcaaggt tgccttagca tcgcccacta ctggaatagc cgttttgaca
    10561 actttaccaa tttctgcggg atcaatatca atatgtgcta tcgtcgcatt tggcgcatag
    10621 gttgttggat ttcctgttag gcggtcatcg aatcgcgctc caatattgat aatgcaatca
    10681 gcctgatcca tagccatatt agcagcatat gaaccgtgca tcccccccat agccaaggat
    10741 aattcatgct caatgggcat tgcacctaat ccaaggaggg ttgaaactac tggaatacgg
    10801 tagcgctctg caaatgcaac taattcttta ttggcatctg cataattgac accaccacca
    10861 gccaatataa ctggtttttg agcttggcta agttgtttca aaattttctt aacctgcaaa
    10921 ccatttggtt caatagttgg ctgataactt ggcaaatgta gagttgggtc atgataaaaa
    10981 tcaacaactc tttcttggat gtcttttgga acatcaataa caacgggacc cggtctccct
    11041 gttgttgcaa tatggagagc ttctgtgatg actctaggaa tatctgccgt atcccgaatt
    11101 tggtaattgt acttggtaat cggcattgtc atccccaaga catctgcttc ctgaaaagca
    11161 tctttaccga taccacgtgt tgcgacttga ccagtaaaaa ctaaaagagg aacactgtca
    11221 cccattgcgt ccgcaattcc agtaatagca ttggtagctc ctggcccaga ggttacaata
    11281 gctacgccca cctttcctga tgatttagcg tatccttcgg cctcgtggac cgctccttgt
    11341 tcatgacgag ccaaaatatg ttggattccc tcatattgat agatagcatc atatagaggc
    11401 aaaactgctc caccaggata accaaaaaca aggtctactc ctagctgatg cagggtgtcc
    11461 aaaatgagat aggaccccga acgcggttgg tctagttgaa tttcttgcac gaatattcct
    11521 cctctctgca aatttgttat ctactataat atcacaagga agtagaatgt caagaaattt
    11581 tctgaaaatt cctacatttg ttcgttttct taactatttc ctaacatttc ttaagcaaaa
    11641 cttaagcaaa tcccttgact gacttggttt cagcttgctt tcagaaaaca tcagtatagt
    11701 agttccacta aaaaaattaa atgaggttta ggatatgtat tatgtagtga taccggctta
    11761 ccagccagat gaaaaattaa tccaactatt agaaataatg aaaaacaggc taaattgtca
    11821 ggtcatcgtt gtggatgatg gtagtactgg cgcatccaaa aatattcttg aaatcgccaa
    11881 aacctatgca attgttctcc accatgatgt caataaagga aaaggccaag ccttacgaac
    11941 agcctttagt ttcatccaag atttgagaaa atcaggtgtt atcgtaactg ctgacgtaga
    12001 tggtcaacat gctgtgaatg atatagaccg tgtcgcacgc gccgccatga acctacccag
    12061 tcgcctgatt cttggtgttc gtcaatttac caaagacatt ccctttcgga gtcgttttgg
    12121 taacaaacta accagagtat tgtttagact gcaaactggt gtcaatgtgt ccgataccca
    12181 aacaggcttg cgtggattcc atactgactt aattccattc atgttagata ttgaaggcga
    12241 ccgctatgag tacgagatga acatgttaac tcaggcaagt caacgctata aaattacgga
    12301 agttccaatt caaaccattt atatcgatga taatgccagt tcgcattttc gtcccataaa
    12361 agatggactg ctcatttaca aaaatctctt caaatttgcc ctggcatctt ttggtggatt
    12421 tctagttgac tacggagtgt atgcacttgc cttattgcta ctcactagct taccaactgc
    12481 tctacgctta ttggtcgcaa acacgattgc tcgcatttgt agcgctacct gtaattttgt
    12541 acttaataag aaattagtct ttaacaatga agagagcatc gcacgaacag gcgcaggcta
    12601 ctttacacta gcccttgtcc tcttcctttg cgatacagcc ctcctctatc tcttccacca
    12661 aattctagga ctcaacctct acattgtcaa acttgcagtt ggtatcttcc ttttcctagc
    12721 ctcttggttt gttcaaaaga atattatttt taaagaaagg aaatcatttt cccatgaaat
    12781 tgcttaaaaa accctttgcc tacgcatcca tcttcggaat gcttttgaca ggaggcttta
    12841 cttactccat gttaaaaacc ttcgtgcttt ccgaggctat cacaacggtt gctgcaagta
    12901 atgccacagc caatagccag aacacagcta cttcatcgtc atcaactgcc gcaaccactg
    12961 cagctaatgt ctctacaacg gatactagtt actctgatga taatattcag ataaatttag
    13021 agacgattac caccaataac acaattgttt acgtcgcaga tattcaagtc agctcacccg
    13081 agtatttaaa gacagctcta gcccaaaata cctatggcac gaacgtaaca gccaaaactt
    13141 ctgaaaccgc cgccgcaaac aatgccattc tagcggttaa tggtgactac tacggagcca
    13201 actcaactgg ttatgttatt aagaatgggg ttctgtatcg agacaccgtt cgggacaatg
    13261 cagcttatgg cgacttggcc atctacgcag atggctcatt tgaggtcatt tatgaaaatg
    13321 agattaccgc ccaagaattg attgacaagg gcgttgtcaa cctcttagca tttggtccat
    13381 cattggtcga aaacggcgaa attgtcgtcg atacttcaac agaagtcgga cgtgcaatgt
    13441 catccaaccc tcgttctgct atcgggatta tcgatgaaaa tcattatatc atcgtcgtag
    13501 cagatggacg tacgtctgaa agccaaggac tttctctcta tcaaatggcc gaggttatga
    13561 agcagtatgg tgctcaaact gcctacaatc ttgatggcgg tggttcatct actctttact
    13621 ttaacggtca agttattaac aacccaacaa caaatggaaa tattatctca gaaagggcgg
    13681 tgagtgacat tgtctacatc ggttactaat tcaactacaa gagtttcaag aacgtttatc
    13741 tcctacttag caataacagc tttcctgttt attttcagtc gaatctatga aagtctcagc
    13801 tatggcgaag tctctgtctt tatgcattat atgttctgtg taccattaat aggcggatct
    13861 gtgctactcg gtctgctgca acttatacct actctttcac gtctctctta taatttgtgg
    13921 aattctggcg ttgcaactgt tgcagctggt tgtcttctcc gaggcattat caacctatct
    13981 ggacgttcaa ctaccttgga tcaaccttat tggtacatcg gcgcagcctt cctagttcta
    14041 tcgttagtag gtcttttcat caatccatcc gcaaacaatc gtcgaaaaac gaccacttaa
    14101 tctacaaata aattataagg gcacttaaca ttcttaggtg ccttttactg tagaataaat
    14161 tgttgaaaaa ttcggaaatt tccaattatt tatcaaatct tttatcgttt ctttcatatc
    14221 tatgatataa tgggtaaaat aagataggga gaagaaaatt atgaccgata caaacaaact
    14281 taaagacttt cgacatcgca gctctgttta cgattcgatg gtcaaatcac ccaaccgtgc
    14341 tatgttgcgt gctacaggca tgaccgatga tagctttgaa aaacctattg ttggggttat
    14401 ttcgacatgg gctgaaaaca caccctgtaa catccacctt catgactttg gaaaattagc
    14461 caaagaaggg gtaaaagatg caggtgcctg gcctgtccaa tacggtacca tcaccgttgc
    14521 agacggaatc gctatgggaa ctcctggtat gcgcttctcc ttgacttcac gtgatatcat
    14581 tgcagattct attgaggcag ccatgggagg gcacaacgtg gatgcctttg tcgcaatcgg
    14641 tggctgtgat aaaaacatgc ctggttccat gattgccatc gccaacatgg atatccctgc
    14701 tgtttttgcc tacggcggaa ccattgcgcc aggtaatctc aatggtaaag acatcgactt
    14761 ggtatccgtt tttgaaggaa tcggaaaatg gaacaacggt gacttgaccg ctgaagaggt
    14821 tcgtcaaatc gagtgtaacg cttgccctgg acctggtggt tgcggtggta tgtatactgc
    14881 caataccatg gctactgtca ttgaggtaat gggcatgtcc attcctggct cttcttctca
    14941 ccctgctgaa tctcctgaga agaaggctga tattgaagaa gctggtcggg ctgttgtacg
    15001 catgctagag cttggtatca aaccatctga cattatgacg cgtgaagcct ttgaagatgc
    15061 cattacagtc acaatggcac tcggtggctc aaccaacgct acgctccatc ttctagccat
    15121 cgctcatgct gctaatgttg aattaacact ggaagatttc aacgatttcc aagagcgtgt
    15181 gcctcattta gccgacttaa aaccatcagg aaaatatgtt ttccaagacc tctacaatgt
    15241 cggcggcgtc ccagctgtca tgaaatactt gctcaaaaac ggcttccttc atggcgaccg
    15301 catcacatgt acaggtaaaa ccgttgctga aaaccttgaa gcttttgcag acttgactcc
    15361 aggtcaagat gtcatcatgc cacttgaaaa tccaaaacga gcggatggac ctctgattat
    15421 cttgaaaggt aacctagctc ctgaaggtgc cgttgccaag gtttcaggcg tgaaagtccg
    15481 caatcatacc ggaccagcta aggtatttga ttcggaagaa gaagctatcg aagcagtctt
    15541 gactgacgaa attgtagatg gcgatgtggt tgttgtccgc tatgttggac caaaaggcgg
    15601 tcctggtatg cctgaaatgc tgtccctttc ttctatgatt gtcggaaaag gccaaggtga
    15661 caaggtagcc cttctaacag acggtcgttt ctctggtggt acctatggac tagttgttgg
    15721 acacatcgca cctgaagctc aggatggtgg tccaattgcc tacctccgta caggtgattt
    15781 ggtgacggtt gatcaagata ccaaagaaat caccatgcac gtttctgacc aagaaatcga
    15841 agaacgcaag aaaacaactg ttattccacc actctactct cgtggtgttc ttggaaaata
    15901 cgcccacact gtatcctctg cctctaaagg agccgttacc gacttctgga gaccagaacg
    15961 tacggggaaa aaataaggaa ctcaacgacc ctgtcctcgc ggcagggttt tccaaaagga
    16021 gaaaaaatga aactaaacgt cgaactctcc cgttttcgtg ttaaagaagg caaaaccgtt
    16081 caagtagatg aatggatggc ctttctcaac gagcacatgg aagacaccct tctcactctt
    16141 gaaggagaga aaatgtatgt cgaaaccatc ttccgtgagg tattggacgg acgcgaatac
    16201 ctctattggt actctgtcca agccgaagga ggaattgaag tcgaagattc agaatcctat
    16261 atcgacaaaa aacatttaga atattgggaa gaatgcatcg acccgagcta tggtattgtc
    16321 gatttggatc cgcaagttat catgattccc aagcctgttt atgaaaccat ggaagaacta
    16381 aatagacaat acgatgaaac atttaaaaaa tgagctgctc catgcaactc attttctttt
    16441 tatttcggac tgataccaag tgtaatgcgt ccaagacggc tgatacgggt catcttccaa
    16501 gcaggagacc agacaatctc tacctgtgca tcgtttatac catcaatcgt tttcaaggct
    16561 gcaaccaatt ccccaggcat agtatccgca caagaacaac ctgaatctgt aaaggtcata
    16621 acaaccttac aaaaaccagc ttcatctaaa tgaatctcat aaatcaaacc caaattgtag
    16681 atgtctagtt caatttcagg atcgtaaatg gattccaata cctccaccaa ttgatctgac
    16741 aaaacaaggg ctcgctcatt gatctgaata tcttctctca tttatacaaa cactcctaaa
    16801 acatttataa aaagtattgt atcataatta gttgaaatat acttgtctta tcaaatgttt
    16861 tcaactagtt ttcaaagtgc acgaacctct gaatgctcac cttgaccaat agaaaagata
    16921 tacttatgac cttgagtcaa gctatgatag aagccatgac cagcataaat ctgaaatact
    16981 tgcattgtat cactagactc atctttatag atatgttgat aataaggatt atccgcaaaa
    17041 actgtcttta gatagtcgtt ctccacaggt ctggcagtcc cttctacacg cactacctgg
    17101 ataaggtacc cttcctcaga aatagccgtc atcgcaactc tttgatcccc tatcaattga
    17161 tcatagaaat gcgtttctgg gcttgtcata aagaaaatcc cttcttcgtt tgctgcagtg
    17221 atatgagcat gacgagcatg tggatttcca tactcatcca aagtagcaaa aactccaacc
    17281 ttcatatctt ccaaaatatg cataatatct tttagttcca tggtatcacc ccttgaaaac
    17341 acctattttc taaagtatat caaaaacaag cgaaaatatt aagtggaata ttgatagaat
    17401 tggtgaaagt gtttcatgaa caactcacta ctgacagaac cattaaagaa ggtagctatg
    17461 tttgcttcat tttcaacctt caaaggttcc tcaacccttt tgatttccaa ttggtatgga
    17521 gttgactact gaaccgatta tgacaacaat caaaatccgt cgtaactata aaaactacct
    17581 aattataaaa aagcgactct tgagtgcttt gaaacgttga tatattgcgg tatattaacg
    17641 tttactaaac tggcttgctt tacgtgcttt ctaaagacct gattttgggt gacaaaaatc
    17701 aaatatcagt aaaagcctat taaatcaatg tcaatatcat acttatttga aatttcttac
    17761 ttatatgtaa atctttaaag caagagatta ccatttgatt accaaaaaca tatttttgga
    17821 gcttatgtaa tttagaaaaa caccccatgg tatgaaccat gaagtgttgt aaatgctgta
    17881 ttgtagctgg ttcttaacgt tttgagaact ggctagcttt acgtgctttc ttaagacctg
    17941 gttttaaaaa cttttgtttg aataaatttc aaaagctttt aatatcaacg ttttcacggg
    18001 ttttcaaaac tcgaaatttc atccaatttc aactaatttc atttgaatgg tgtgaatttt
    18061 acccccaaaa tacacgaatt gagctaaatt ataccgaaaa gttcacacca tgattctatt
    18121 atgaaattat tacaaatatg gttgttaaat cattgttttg gtttctgcat actcataaat
    18181 cctgattcgt ttgttttcat tagttgttct tactttaaaa ataaaatagt ggtcacgacc
    18241 atcagttgac ttacttttgt gcaatcgaaa gtaatttttt tgtttcatcg ccatagttag
    18301 caatatatca tcaattaaaa atgtaagagg gtcaacacct ttatagtacc tgttaaaatc
    18361 cttctcaaat tgaagatatg aatcagaaaa ataatcgaat tgaatcatgt cttcatagat
    18421 agcttttcta ttcatctaat acctccacta aactaatttc aattatttca gtaatttgaa
    18481 tcaattcata cttttgtatt gtttttatgg taatttcttt tacacttagt tcggttattt
    18541 tgccttgata cgcttccctt ttaccattac gtactacaat gaacattccc tgtgattgcg
    18601 aagagtataa ttgagtaatg taagttagta tctcaatttt tgatagctta gatgacatat
    18661 ctattctaat cttatcatcc atcaatgagc tggtatgttc agatagaaag aatcccatcc
    18721 atttctgcat acctcggtct tgataatctc ttgccgattg gtacggcaaa taacttctat
    18781 caatcatttc aaaccatcta aaccgccagc agaatgtcca ccaatcaatt tacttcttgc
    18841 aatactacgt gatgcattcg taagtacgtt agctttttgg agcattgtaa atccaaattg
    18901 attacgtatc gaatctattg cggtttgtaa tctctctttc tttagtaatt cttcagggtc
    18961 atcaaataaa ttcaggattg tcagattttc atcaacgaac gaagagtagg atacagctat
    19021 acttctaaca gctccaccgt tgtatttttt cctaaataga gaaattacat acttttgtaa
    19081 atcctcagtt agattagtag gttctatttt catttgtcct ctaaatgatg ttttctcttc
    19141 aaaccacgaa tatccaatgc caatatagac tgttgttgtt tttttatgaa tacctcgcaa
    19201 gcgaatagca acctgttcag ccatctcttt taaaacaagt tctatctcat actgcttaaa
    19261 atagtctctc ggaagaactt gagagtttcc taatccctta gactttggtt tatatggtat
    19321 gtgtacatta ctttcatcaa tgccatttgc atgaaaccat aattgaacac catatatacc
    19381 aaattctttt tttaacaggt ctggattata tttagccaat tctttaatag tatagattcc
    19441 aagtttttca agtctttttt gaattgctgt tccaattccc caaaagtcgg ttaatttaga
    19501 aattccccaa acttttgtag tcacatcttc ataagaccaa ttggctctca tattatgtgt
    19561 tttcttagcc tcgttatcta atgctaattt tgcaagtaaa ggattggcat ttgacatccc
    19621 aattgtagag tagacacctg ttttgcgata gatatcccgt tgtattctcg cactaagcat
    19681 atctaattta tcttttcgac taatcgtctg attcggaata aaataattta aagaagatgt
    19741 caagtctata aatccttcat cgattgagta aggcattata tcttcaatac ttccatattg
    19801 ttgaaaaata tgttgaatat tgatattttt ctcaatatat aaatccatac gtggcggaac
    19861 aataactgtt gctactgccc attcctcaat gaatttaaca taagcagggt ctgttggtaa
    19921 gccctgtctt tttgcattat agtaagaaaa ttttcttgta ttgatatcaa aaggtaaatc
    19981 ataggctctt ccaacgtttg atttaccaaa aacttcttta aatactggtg aagatgctaa
    20041 tatcaatcca ccagaattgt ctgccctact catcacacat aaggatgtct ttaatgggtg
    20101 caagccccga ctgacacatt caacacttgc ataaaacgat ttcatgtcaa taaatgcaat
    20161 atcgctctct ggttctctac tgtaatcaat catactcttt acattgtgta atattatttt
    20221 tctccagtaa agattttagc cattcttttt ctgcatctga ttcaacacta ctgaaatcag
    20281 cagacttgat atcatagaaa tcttcaatat agtcaataat ttctctctcg tttgtaaata
    20341 tcagagcttg attcttctca gctatagttg attttgtaat atgaaagtta ttctcttcaa
    20401 accatttttg gcatgctgaa agtacagtta aggtgacatc tattttttca ctataattat
    20461 tgaacacaat tctttgattc tcaattgcta agtgtttgtt ctcgctacct aatggaaata
    20521 ttacttttgt tttcatctat tgcacctctt cgattgggat aaaatgcccg actattttcc
    20581 cgacaatttt aaattcatct ccctcataag catacatgtc tgcatacttg ggattgatgg
    20641 acacaatcct gtacctatct ttttctttga acaaacgctt gatataagct tttccattca
    20701 atgaaattgc atacactgct ccatcataat caaatcctga atccgaaaac aatgcgactt
    20761 ccccatcaaa atacttcggt tccattgatt ctcctgaaat aaaagaagca aagtcatagt
    20821 tatattcctt atcggaatat acagtttgaa aactatattc gttgtagtaa ctctctccgt
    20881 atccagcaga caaaggaact tcttccagca cttggatggg atataatgga atgaccttat
    20941 tggctacttc ttcttcaatt tggtttttta ataattcttc gacaacctgt tcagctgtat
    21001 tcttattttt ttgattcaat ttaagataat tggatagaat catatatcca tgttcgaaat
    21061 cggtgacttc tactccaaat aggtcagcta aacgcttcaa atttgctcta tttggttttg
    21121 cacgtcccaa ttcccaactc gtataagagg ctcggtttat ccccagtttt tgagcaatat
    21181 cttgttgggt taaaccactt tcttttctta gttgctttaa gcgttctcct gaaaacataa
    21241 acacctccaa aatgtgtttt attaaacaca ttatacaaaa catattccca tttgtccata
    21301 agaaaacaac caagaaattt ctcggttgtc aacattattt atttaatgtt actgtttcac
    21361 cactatctcc tgctaaatca ggtattgtta cagaatctcc tgaaactgaa tatttcagct
    21421 ccgttccatt gctatcactg ataatctgtt tgtctgtatc aattgttact ttctcagaat
    21481 agaggtactt cacattattg cctctctctg aaaggagatt gatattaagt atattttcat
    21541 caccactaac ttttagttcg taatgcaaag ttaattcttc gtcttcgaat cccatttcag
    21601 aaccatcaat accgcctttt tcgtatgaat atgttcccga tagctgacta tcattggaat
    21661 tcgattgatt accacaagca caaaggacaa ttgtcagcgt tagagtgagt agggtcaata
    21721 ttagtttctt tatgttcatc agtctgctct tttctttaat tgtttgaaat tagattttct
    21781 agctcagaat catttggagc tgtagatggg taagtttcaa atactagctt gggtacaatg
//
LOCUS       DATUCG010000022        21683 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.023, whole genome shotgun sequence.
ACCESSION   DATUCG010000022 DATUCG010000000
VERSION     DATUCG010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21683
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.023"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            1..449
                     /locus_tag="VCA20_000689"
                     /pseudo
     CDS             1..449
                     /locus_tag="VCA20_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941577.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(614..1969)
                     /locus_tag="VCA20_000690"
     CDS             complement(614..1969)
                     /locus_tag="VCA20_000690"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEP1834543.1"
                     /translation="MKVIKFGGSSLASAGQLEKVFNIIQSDSERRFVVVSAPGKRNAE
                     DTKVTDALIKYYKEYVNGKDVTASQEWIINRYQAMVDELGFTASSMKKIADSIISLAS
                     LPIDDNEFLYDAFLAAGEDNNAKLIAEYFTHRGLPARYVHPKKAGIIVSSEPGNARIL
                     PSSYDKIEELRDTDEVLIIPGFFGVTVDNQICTFSRGGSDITGSIIAAGVKADLYENF
                     TDVDGIFAAHPGIIKNPHSIKELTYREMRELAYAGFSVLHDEALLPAYRGRIPLVIKN
                     TNNPTHPGTRIVHKHTEQTVPVVGIAADDGFVSINLSKYLMNREVGFGRKVLQILEDF
                     NIRWEHMPTGIDDLSIVVRERELTPIKEEEILRQLNTKMEVDKAEIEHGLSIIMIVGE
                     NMKSHVGVTATATTALSKQNVNLAMISQGASEVSVMFVVKTEEKKRALHALYEAFFNE
                     Q"
     gene            2146..2787
                     /locus_tag="VCA20_000691"
     CDS             2146..2787
                     /locus_tag="VCA20_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEP1834544.1"
                     /translation="MVKAIIFDMDGVLFDTETFYFQRRADFLATKGLSVDHLDPSIFV
                     GGRASQIWQRILGDDYDNWDVPALEEEYRVYKEKRPTPYAERIFPEVKAVLERLTIQG
                     IPVVLASNTDHSEIERALLDAGIRSHFSHVFSAMDCEASKPDPTVYMKAASATGVAKS
                     DILVFEDSAKGIEAAKKAGLTVWAIRDRHYGIDQSKADKLVDNLEEAFDLLRF"
     gene            3224..4015
                     /locus_tag="VCA20_000692"
     CDS             3224..4015
                     /locus_tag="VCA20_000692"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEP1834545.1"
                     /translation="MAYQTIRYEVIDSVAILTLNRPEVANGFNIPMCEEILEAIRLAE
                     GDASVKILQIQAQGSIFSVGGDLVEMKRAVDDDDIASLVKIAELVNDISFAMKKLSKV
                     VIMVTDGAVAGAAANMAVAADFVIASSKTKFIQAFVGVGLAPDAGGLFLLSRAIGANR
                     ASHLAMTGEGLSADKALEYGIIYKLAEPEKLEKTVAQVIKKLLRGSVNSYAAIKQLTW
                     ESQFKDWESYRRLELDLQETLAYKEDFKEGVRAHAERRRPVFTGD"
     gene            4095..4529
                     /locus_tag="VCA20_000693"
     CDS             4095..4529
                     /locus_tag="VCA20_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEP1834546.1"
                     /translation="MEDPKVNEYLTAIFNNVLVIEETSLRGSRFNDISIKEMHTIDVI
                     GSIDGATPSDVARTLMVTLGTVTTSLNNLERKGYVERIRSTKDRRVVHLYLTKKGRLV
                     YRLHQRFHNEMVKQITDGMDETEYQVMKKGLMKLYHFLEDLK"
     gene            4526..5503
                     /locus_tag="VCA20_000694"
     CDS             4526..5503
                     /locus_tag="VCA20_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEP1834547.1"
                     /translation="MRNHAKISQVAHYLPKKIVTNDDLAQRMETSDEWIRSRTGIGQR
                     HIVTGETTSDLASQVARKLLGKSQLDASEIDFIIVATITPDASMPSTAAMVQAAIGAK
                     KAFAYDLVAACSGFVFALSTAEKLLASGVYKRGLVIGAETLSRSVDWSDRSTAVLFGD
                     GAGGALLEACEQPTFLAEILRTDGGRGASLTAGIDQKETPFSTQSRQQPFIQMEGRAI
                     FEFATRDVTATMAELLEQADMTVDCVDYFLLHQANIRILDKMARKLGVAREKFPANMD
                     KYGNTSAASLPILLSECVESGMLRLDGSQTVLMAGFGGGLTWGTLLLQL"
     gene            5563..5787
                     /locus_tag="VCA20_000695"
     CDS             5563..5787
                     /locus_tag="VCA20_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEP1834548.1"
                     /translation="MAVFEKVQEIIVEELGKDAEEVTLTTTFEDLDADSLDLFQVISE
                     IEDAFDIQIDTEEGLTTVGDLVAYVEEKTK"
     gene            5957..6922
                     /gene="fabK"
                     /locus_tag="VCA20_000696"
     CDS             5957..6922
                     /gene="fabK"
                     /locus_tag="VCA20_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HEP1834549.1"
                     /translation="MKTKITELLDIKYPIFQGGMAWVADGDLAGAVSNAGGLGIIGGG
                     NAPKEVVKANIDKVKSITDKPFGVNIMLLSPFADDIVDLVIEEGVKVVTTGAGNPGKY
                     MERLHAAGITVIPVVPSVALAKRMEKLGVDAVIAEGMEAGGHIGKLTTMTLVRQVVEA
                     VSIPVIAAGGIADGAGAAAAFMLGAEAVQVGTRFVVATESNAHQAYKEKVLKAKDIDT
                     TVSASIVGHPVRAIKNKLSSAYAAAEKDFLAGKISADAIEELGAGALRNAVVDGDVTN
                     GSVMAGQIAGLVSKEESCEDILKDIYYGAAKVIREEASRWASVGE"
     gene            6924..7844
                     /gene="fabD"
                     /locus_tag="VCA20_000697"
     CDS             6924..7844
                     /gene="fabD"
                     /locus_tag="VCA20_000697"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEP1834550.1"
                     /translation="MTKTAFLFAGQGSQTVGMARDLYEYYPIVRETFDQASQILGYDI
                     RDLIDNQEDKLHQTRYTQPAILTTSVAIFRLLEEEGIQPDMVAGLSLGEYSALVASGA
                     LAFEDAIALIAKRGEYMETAAPAGTGKMVAVLNTDVNLIEEICSTVTSGIVSPANYNT
                     PAQIVIGGEVAAVDEAVELLKEAGVKRMIPLNVSGPFHTALLQPASEKLVQALEQVEF
                     ADFQVELVGNTEAKVMKKEDIKSLLPRQVMEPVRFYESIATMQEAGVINFIEIGPGKV
                     LSGFVKKIDKTAHVAAIEDIEGLQIVLNKS"
     gene            7854..8588
                     /gene="fabG"
                     /locus_tag="VCA20_000698"
     CDS             7854..8588
                     /gene="fabG"
                     /locus_tag="VCA20_000698"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011225529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEP1834551.1"
                     /translation="MELTNKNVFVTGSSRGIGLAIAHKFASLGANVVLNGRGQLGQDL
                     LDSFADYGVKVLAISGDISSAQDAKRMVAEAIETLGSVDILVNNAGITKDGMALRMTE
                     EDFDTVLKVNLTGTFNMTQAVLKPMTKAREGAIINLSSVSGLIGNAGQANYSASKAGV
                     IGFTKAIAREVAGRNVRVNAIAPGFIQSDMTDVLSDKIKEAMTAQIPMKRFGATEEVA
                     DVAVFLAKQEYLTGQVIAVDGGLTMQ"
     gene            9038..10273
                     /gene="fabF"
                     /locus_tag="VCA20_000699"
     CDS             9038..10273
                     /gene="fabF"
                     /locus_tag="VCA20_000699"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEP1834552.1"
                     /translation="MTKLNRVVVTGYGLTSPIGNTPEEFWNSLKAGKIGIGKITKFDT
                     SEYSVLNAAELKGFPFDKYFVKKDTNRYDNYSLYALYAAKEAIANAQLDTETVDSDRF
                     GVILSTGIGGILEIEEQVARMNEKGPKRIRPMALPKALPNMAAGNIAMQVGANGVCKC
                     IITACASSNDALGEAFREIKFGFQDVMLAGGAEAAITPFAIGGFQALTAMSTTEDPER
                     ASIPFDKDRNGFVMGEGSAVLVLESLEHAEERGATILAEIVGYGNTCDAYHMTSPHPE
                     GLGAIKAMKLAISEAGLEPADIDYINAHGTSTPANEKGESQAIVSVFGKNTPVSSTKS
                     FTGHLLGAAGAVEAAAVIEAMRHSYAPKTAGTTELSEDIEADVIYGQGRDMEIRHAIS
                     NTFGFGGHNSVIAFKRWEA"
     gene            10274..10750
                     /locus_tag="VCA20_000700"
     CDS             10274..10750
                     /locus_tag="VCA20_000700"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEP1834553.1"
                     /translation="MNITEIKDLMSQFDQSSLREFSYSNAGETLHFSKNQQATVAPSP
                     AVEAPLAPVTPASPAPVPAVEMSEPAPAEAGASPVAEGAVVESPLVGVAYLSPAPDKP
                     AFVAVGDTVKKGQTLMIIEAMKVMNEVPADRDGVVTEILVANQDVVEFGQGLVRIK"
     gene            10747..11169
                     /gene="fabZ"
                     /locus_tag="VCA20_000701"
     CDS             10747..11169
                     /gene="fabZ"
                     /locus_tag="VCA20_000701"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002922202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEP1834554.1"
                     /translation="MIDILKIKEALPHRYPMLLVDRVLEVSEDEIVALKNVTINEPFF
                     NGHFPDYPVMPGVLIMEALAQTAGVLELSKEENKGKLVFYAGMDKVKFKKQVVPGDQL
                     IMTAKFVKRRGTIAVVEAKAEVDGKLAASGVLTFAIGK"
     gene            11195..12568
                     /locus_tag="VCA20_000702"
     CDS             11195..12568
                     /locus_tag="VCA20_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEP1834555.1"
                     /translation="MFEKILIANRGEIAVRIIRAARELGIATVAVYSEADKEALHTML
                     ADEAVCIGPTRSTDSYLNMQAVISAAVVTGAQAIHPGFGFLSENSKFATLCEEVGIKF
                     IGPSGTVMDTMGDKINARAEMIKAQVPVIPGSDGEVLTSQEALEVAEKIGYPIMLKAS
                     AGGGGKGIRKVEKAEDLVPAFESASSEAKAAFGDGAMYMERVVYPARHIEVQILADQH
                     GHVIHLGERDCSLQRNNQKVLEEAPSIAIGQTMRERIGQAAVRAAQSVGYENAGTIEF
                     LLDEAKGEFYFMEMNTRVQVEHPVTEFVTGVDIVKEQIKIAAGQELSVRQEDVKITGH
                     AIECRINAENPAFNFAPSPGKISNLYLPSGGVGLRVDSAVYPGYTIPPYYDSMIAKVI
                     VHGENRFEALMKMQRALYELEIDGVVTNTDFQLDLISDKRVVAGDYDTAYLMEEFLPR
                     YQEELKK"
     gene            12583..13449
                     /locus_tag="VCA20_000703"
     CDS             12583..13449
                     /locus_tag="VCA20_000703"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEP1834556.1"
                     /translation="MALFRKKDKYIRINPNRSRIESAPQAKPEVPDELFSKCPACKEI
                     LYKKDLGPEKTCQHCSYNFRITAQERLALTVDEGSFEELFTGIETTNPLDFPNYLEKL
                     AATRQKTGLDEAVLTGKATIGGQPVALGIMDSHFIMASMGTVVGEKITRLFELAIEER
                     LPVVLFTASGGARMQEGIMSLMQMAKISAAVKRHSNAGLFYLTVLTDPTTGGVTASFA
                     MEGDIILAEPQTLVGFAGRRVIESTVRENLPDDFQKAEFLQEHGFVDAIVKRQDLPAT
                     ISRLLRMHGGVR"
     gene            13446..14219
                     /locus_tag="VCA20_000704"
     CDS             13446..14219
                     /locus_tag="VCA20_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEP1834557.1"
                     /translation="MTSDVARMIRLARDQARLTTLDYATQLFDDFIELHGDRNFRDDE
                     AVVGGIGFLAGQPVTVIGIQKGKSLQDNLKRNFGQPHPEGYRKVLRLMKQAEKFGRPV
                     VTFINTAGAYPGVGAEERGQGEAIARNLMEMSDLKVPIIAIIIGEGGSGGALALAVAD
                     QVWMLENSMYAVLSPEGFASILWKDGSRAMEAAELMKITSYELEKLQVVDRVVLENGR
                     ATKEVLADIKENLISQLAQLQALPLDELLENRYQRFRKY"
     gene            14283..14756
                     /locus_tag="VCA20_000705"
     CDS             14283..14756
                     /locus_tag="VCA20_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEP1834558.1"
                     /translation="MLVKADLSNVEELLPIQHRAFAALYETYQDQYNPAIETMDYFQS
                     HFARPNCTYYKIVEEGHTVGLVRTTVAEDADQGWLGLIGIDPDHRGKAYGHKAMLELE
                     SLYPTVRRWVLCTVLQESKLVSFYEKLGYKAIKTEPEQEGMDMVYMEKWIGDSDA"
     gene            14749..15381
                     /locus_tag="VCA20_000706"
     CDS             14749..15381
                     /locus_tag="VCA20_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834559.1"
                     /translation="MLEQCRRYLDYQGVHYCKPEKAGALAGDMIALRNVAQAARKEFQ
                     TLSLVLAEHVSPFVPERVSQWMNQAQICRPHFWTYYRFPSASLTDSALAIRLYGKGTE
                     AGISVEVSFIERKKVETSLLQQNRVLNCSIAAPLYYWVQEGQVFYRLDGTEENRQSLL
                     QAVEAGRIRKVLVKYDVPFRTVASEEELLERLIQGFSLLEPYLKMTHPAE"
     gene            15559..15804
                     /locus_tag="VCA20_000707"
     CDS             15559..15804
                     /locus_tag="VCA20_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834560.1"
                     /translation="MEKLSQGLEWIGRKIWLLLRIFGLLVLAVLPDLILEGSKSQVTL
                     VIVGLMTLGLLVFFWWRAEKMICQSGTLTFLLGTVWR"
     gene            15825..16217
                     /locus_tag="VCA20_000708"
     CDS             15825..16217
                     /locus_tag="VCA20_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEP1834561.1"
                     /translation="MQLTDMLGYYLLELQGVTTTENDASIIEFLKGIPFRLALLTTAF
                     LPAVVEEVIFRGYFFKKLFGSQVLLGIVVSSLVFGSFHGPTDLGSWLIYAGSGIILSL
                     LYYKSRYLIYPIIVHLVNNFIATVFYYI"
     gene            complement(16271..17197)
                     /locus_tag="VCA20_000709"
     CDS             complement(16271..17197)
                     /locus_tag="VCA20_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEP1834562.1"
                     /translation="MNLSKEKQLFHHYYANWLRIYKEGAIREVTLKKYRMTLQWLIRL
                     APSLLLEDLNRVRYQELINSYAKTHERQTTMDFHHQLKGAILDAVDEGIIERDPTRKV
                     IIKGRLPRKKKTKYLNQFELHQLIQELRLGEELNWDWLILLIAKTGIRFSEALALTTQ
                     DFDFHRQLLTIDKTWNYKGDGGFLPTKNKSSIRKIQLDWQTSTQFANLLQHTQPDDLL
                     FVKKERIYNSTINAILEKRCLSANLPVVTIHALRHTHASILLFAGVSIASVARRLGHA
                     SITTTQKTYLHIIRELENQDVDLIMRSLSGLC"
     gene            17318..17893
                     /locus_tag="VCA20_000710"
     CDS             17318..17893
                     /locus_tag="VCA20_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEP1834563.1"
                     /translation="MKLEELALFTGGSLQVRLDTTSWEDARDYILYNQQHHQLDGYEV
                     IEEVIDSRTVTTDREVTLLEEGDLLFSLLSGKAVLVRAEHAGLLYTQNYIKIEPIAKL
                     DKAFLLYLINESSAIRRQFYQSLQGSEVMKYTVKQLKSLQLDALPPLDSQQKLGKIYL
                     DGLALRQKRQAYAQGDFLRLTYLLKEVYAHE"
     gene            17886..21092
                     /locus_tag="VCA20_000711"
     CDS             17886..21092
                     /locus_tag="VCA20_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEP1834564.1"
                     /translation="MNELAFETELIQYITTGTITQPEHLEGLEWSVSDSSTLYAVKSK
                     LWTYEPSIKTTEQLWENFRNILWQHNQHRLDKPLSDTEFAQVKQIISNLKTPYEAGQF
                     LYGISGVSELEITLDDGRHTFLTVFDQRKIGGGDTVYQVVNQIRRAPKIPGRRECIFD
                     TTLLINGLPIIQIEEKKDTVDVDKALNQMHQYIQERQYTDIFSTLQILVAIAPNNVRY
                     MANTTAEKFNKDFAFHWQRKSDGSKVRNWKEFADSMLSIPMAHQMATNYMILDGTPQK
                     QMLKVMRPYQVYATQAVIEGIKKVDFELGCNKVGYIWHTTGSGKTITSFKTAWLASRM
                     PKVDKVVFLVDRIALTNQTKDDYLAYDPEAGENSFGSIRETQNTTALARQLTSKDSGI
                     IVTSVQKLDTLVKRKSFKSPDKHIVFIVDEAHRSTGGDSFAGIQKAFKKAAWIGYTGT
                     PNLDDTGASLRTQDIFGPLLHAYTIKNAISDRNVLGFKVDFETTIDQEKLKTTYLPDF
                     YRQKYPKWTSAQIEEKIANLSPDDMDDAVTSSVYDENPEHVRLVVEDVFKHWANRSGS
                     GRYNALFTTHVGGGRASTPMAMMYFREFQRINQLHREQGGLVLKVGVTFALNTQNNDS
                     MVESNTGLLEAITIYNTEFGTSFGLSDVASYTEDLVSRLNRTAKDKNYLDLVIVVDQL
                     LTGFNAPELNTLYVDRTLKGASLIQAYSRTNRVEDMDKKPFGRVVNYRWPAHNEKLMN
                     QALGIYADDSAANLSDAEQKNNNEKNGLVAKPFKEVFAEVKQTVDQLRELTGDFNDIP
                     KSEKKREAMLELLRAYNQGLSKLKQYQPEEVDGVIEGFDYNQPDRFIESLGMSVEEEK
                     LLTTVLANELKRTLAKEQKVSIHQIELKMTHIKEVTIDYDKLTELVELLLNQVHEEKM
                     VEAAATRDELDRFASGLEDLNYADQIRQTGDAIYRGDFPTKDMNFAYPVKGIDSQKII
                     QEASHVYIDKKLFAFRIQWGLTEHIRNQKLRELLARHRYGQNDLDDTGHLRTLIKEAS
                     RDYQVWAEDDAVKGLSKIQYRNQLRQALYDLADDLVGQ"
     gene            21200..>21683
                     /locus_tag="VCA20_000712"
     CDS             21200..>21683
                     /locus_tag="VCA20_000712"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEP1834565.1"
                     /translation="MTKEKSTVPRLRFPGFTDAWKQRKLGEVGYAKSGVGFPEKEQGG
                     TTGIPFFKVSDMNTNGNENELVVANNYVTDEQISKRKWRVITEVPAIFFAKVGAAVML
                     NRKRLIRHPFLLDNNTMTYVFNHTQWDTNFGKTLFETIDLIQLTQVGALPSYNSSDVE
                     N"
BASE COUNT         6254 a         4151 c         5308 g         5970 t
ORIGIN      
        1 gctcccttga tggacgagtt ctttgactgg tgtcgtgagc aatttgtttt gccaggctcc
       61 aaattgggta ctgctttgga gtatagcctc aaatacgaaa ccaccttccg aaccgttctc
      121 tcggatggtg acttggtcct gtctaacaac atggctgaaa gggctatgaa gaccttggtg
      181 atgggcagaa aaattggttg ttctcccaaa gttttgaggg agccaagtcg acagctatca
      241 ttctgagtct tttggaaacg gccaagagac acggacttga tgcagagaaa tatatgatct
      301 atcttctaga acacttacct aacgaggaga tgctcgcaaa aaaagaggtg ctagaggcct
      361 atttgccatg ggttaaaaat attaagagag catgtaaatt gaaaaaggtt ccagagtcga
      421 caggactttg gagcttggag atagagtgaa cgaagttcgt ttctccaaga gcagatatac
      481 cctgctactt tcttatttta ttgtagcagg ctgtcaagct tcactggagc ttgacactca
      541 tcaaatcaag tcaacaacgt ctggctttga ttttcgaaga gtatgaagag gggactgacc
      601 gacctcgttg tgattattgc tcgttaaaaa aagcttcata gagtgcgtgc agggcgcgtt
      661 tcttttcttc ggttttgaca acgaacataa cagaaacttc actggctccc tgtgaaatca
      721 ttgccaagtt gacattttgt ttagagaggg cagtggtagc ggtagctgtt acaccgacat
      781 ggctcttcat attttcacca acaatcatga taatagataa accatgttcg atttcggcct
      841 tgtcaacttc cattttcgta ttgagttgac gaaggatctc ttcttccttg ataggagtca
      901 attcacgctc acggactacg atggataaat cgtcaatacc ggtcggcatg tgctcccagc
      961 gaatattgaa atcttcgaga atttgcaata ccttgcgacc aaaaccaact tcacggttca
     1021 tcaagtattt ggacaagtta atgctgacaa aaccatcgtc tgcagcaata ccgacaacag
     1081 gaactgtttg ctcggtatgt ttgtgtacaa ttcgggtacc agggtgagtt gggttattgg
     1141 tgtttttaat aaccaatgga atccgcccgc gataggcagg aaggagggct tcatcgtgga
     1201 ggacggagaa acctgcataa gctaattctc gcatttcacg ataggtcaat tctttgattg
     1261 aatgtggatt tttaatgata ccaggatggg cagcaaaaat accatcgaca tctgtaaagt
     1321 tttcatagag gtcagccttg actccggcag caatgatgga acctgtaata tccgatccac
     1381 ctcgtgagaa ggtacaaatc tggttatcaa cagttacacc gaagaatcct gggataatga
     1441 gtacttcatc tgtatcacga agctcttcga ttttgtcata acttgaaggc aaaatgcgag
     1501 catttcctgg ttcggagctg acaataatac cagctttttt aggatggaca tagcgtgcgg
     1561 gcaatcctct gtgggtaaaa tattcggcaa tcaatttggc attattatct tcaccagctg
     1621 ctaagaatgc gtcgtaaagg aattcgttat catcaattgg taggctggcc aaagagatga
     1681 tgctgtctgc gattttcttc atgctgctag ctgtgaaacc aagttcgtca accattgctt
     1741 gataacggtt gatgatccat tcttgactgg cagtcacatc ttttccgtta acatattctt
     1801 tataatattt aatcaacgca tcagtaactt tagtatcctc ggcattgcgt tttccaggtg
     1861 cagacaccac aacaaagcga cgttctgaat cagattgaat aatattgaaa actttttcta
     1921 actgtccagc tgaagcaagt gagcttcccc caaatttaat aactttcatt ttcatctcct
     1981 tggatatttt tttctattat agcagaatga tatgaaattg tcagtatgtt cagacaaatc
     2041 ttgccaagaa ttttatcgaa aaacgaacaa gagacaagta tgaaaatttc agaaaaacat
     2101 ttttttcttt caactgtgtt acaattaaag taataggagg cgagtatggt aaaagcaatt
     2161 attttcgaca tggatggtgt actttttgat acagaaacat tctattttca acgtcgagcg
     2221 gattttttag cgacaaaagg tttatcggtt gatcatttgg atccatctat atttgtcggt
     2281 ggaagggcta gtcaaatttg gcaacggatt ttaggggatg attatgataa ctgggatgtc
     2341 cctgccttgg aagaagaata tcgtgtgtat aaagaaaagc gaccaacccc ttatgcagag
     2401 aggatttttc ctgaagttaa agcggtattg gaaagactaa ctatccaagg tattcctgta
     2461 gttttggctt caaataccga ccattcagaa atcgaacgtg ctctactaga tgctggtata
     2521 cgctctcact ttagtcatgt tttctcagcg atggattgtg aggcctcaaa accagatcct
     2581 acagtttata tgaaagcagc cagtgcaaca ggagtagcta aatcggacat tttagtattt
     2641 gaagatagtg cgaaggggat tgaagcggct aaaaaagcag gtttgacagt ttgggctatt
     2701 cgagaccggc attatggaat tgatcagtcg aaagcagaca aattggtaga caatcttgaa
     2761 gaggcctttg acttattgcg attttaagaa atatgatatt gtagtccaag tattagttct
     2821 tggtgaaaaa tggtatttct agttcagaat ccagttagat cttccaaaag tattaacgtg
     2881 aaaaaatgaa agttgtaaat taaacttaga gcctatattt acagttcaga cttgcatatt
     2941 gaggttgttt tttcaaaata cagtcagtat ttcccgcaaa aactaccttg atgagtgaaa
     3001 aagccaaggc gcttttcgtg tgaatatagg ttcttagaca cgttattttc tagcatatct
     3061 aaattttata ttgcgataat ggcctccatc tttttcggac gtcacacttt acttgctaga
     3121 aaaaagtgac aattataaaa actgtgttac aatggaaaac aagaatttga agtttgatat
     3181 tcaaatcttt taattatcaa actattttga aaggagcaag ggaatggcct accaaacaat
     3241 tcgttatgaa gtaattgatt ccgtagcgat tctaacttta aacagaccgg aagtggcaaa
     3301 tggttttaat attcctatgt gtgaagaaat cttagaagca atccgccttg cagaagggga
     3361 tgcctccgtc aaaattttac aaattcaagc acagggttcc attttctccg tgggtggaga
     3421 tttagttgag atgaagcgtg cagttgatga tgatgatatt gcttctttag taaaaatagc
     3481 agagctggtg aatgatattt cgtttgcgat gaagaaattg tctaaagtag tcattatggt
     3541 gacagatggg gcagtggctg gagccgcggc taatatggca gtggcagctg attttgttat
     3601 tgcctccagc aagacaaaat tcatccaagc ctttgtaggc gttggacttg cgccggatgc
     3661 gggggggctt tttctactga gtcgtgctat tggggctaat cgtgctagtc acttggcgat
     3721 gactggagag ggcttatcag cggataaggc tttagaatat gggattattt ataaattggc
     3781 agagccagaa aaactagaaa agacggttgc ccaggtcata aaaaaactct tgcgaggctc
     3841 agtaaattct tatgcagcaa tcaaacaact gacatgggaa agtcaattta aagactggga
     3901 gtcttatcgc cgattagagc tggatttgca agaaacatta gcctataaag aggattttaa
     3961 agagggggtt cgagcgcatg cagaacgacg tagaccagtg tttacgggag attaattttt
     4021 aaattagtaa aaaatgtttg acaatcaaag ttttatggtt tatactttga ctatcaaagt
     4081 aaaggaggcc tgttttggaa gatccaaaag taaatgaata tctaacggcc atttttaaca
     4141 atgtgttggt gattgaagaa acaagtctgc gtggtagccg attcaatgat atttcgatta
     4201 aggaaatgca cacgattgat gtgataggaa gtattgatgg agctactccg agtgatgttg
     4261 ctcggacttt gatggtaacc ttaggtactg ttacaactag cttgaacaat ttggaaagaa
     4321 aaggatatgt tgagcgtatt cgttcgacca aagatcggcg agttgttcat ctctatctga
     4381 ccaaaaaagg tcgactggtc tatcgtttgc atcaacgatt tcacaatgaa atggtgaaac
     4441 agattacaga tggtatggat gaaacagagt atcaggtaat gaagaaagga ctcatgaaac
     4501 tctatcactt cttggaggat ttgaaatgag aaaccatgct aaaatcagtc aggtggccca
     4561 ctatcttccg aagaagattg tgaccaatga tgatttggct caacgaatgg agaccagtga
     4621 tgagtggatt cgtagccgta caggtatcgg tcaacgacac attgttactg gagagacgac
     4681 gagtgatttg gctagtcaag tggctagaaa attactggga aagtctcagc tagatgctag
     4741 tgaaattgac ttcatcatcg tagcaacgat tactccagat gcttcaatgc catctacggc
     4801 agctatggtt caagcggcaa ttggcgcgaa aaaggctttc gcctatgatc tcgttgcagc
     4861 ttgttcagga tttgtttttg cgctatcaac agcggaaaaa ttgcttgctt caggagtcta
     4921 taagagagga cttgtcattg gtgcagagac cctatctagg tctgtcgact ggtcggatcg
     4981 atcaacggct gttttatttg gagatggtgc tggaggagct ttgctggaag cgtgtgaaca
     5041 accgaccttt ttagcagaaa tcttgcgaac ggatggcggt cgaggtgcta gtctgacagc
     5101 agggatagac cagaaagaga cgccattctc gacacaaagc cgtcaacaac cctttatcca
     5161 aatggagggc agagctatat ttgaatttgc tacgagggat gttacggcaa ccatggcaga
     5221 gctgctagaa caagctgata tgactgtgga ttgcgttgat tatttcttgc tccatcaagc
     5281 caatatacgt atcctagata agatggctcg taaattggga gtggcgcgtg aaaaatttcc
     5341 agcaaatatg gataaatatg gcaataccag cgcagcaagt cttcctattc tcttatcaga
     5401 atgtgtagag agcggaatgt tgcgattgga tggaagccag actgttctca tggctggttt
     5461 cggtggaggt ctcacatggg gaaccctact cttacaactt tagttaattc cttgccctag
     5521 gcaagattaa aatattttat tttccaaaag gagacacata tcatggcagt atttgaaaaa
     5581 gtacaggaaa tcatcgttga agaattgggt aaggatgctg aagaagtaac ccttacaaca
     5641 acctttgaag atttggatgc agattcattg gatcttttcc aagttatctc tgaaatcgaa
     5701 gatgccttcg acatccaaat tgacactgaa gaaggtttga ctactgtagg cgatttggtt
     5761 gcttatgttg aagaaaaaac aaaataatga ctgttaagga aagtgctatt tagcattttt
     5821 cttaaattaa atactttgat aatcaaaaca ttgaaatgtc aaactatttt tttagtgagg
     5881 gggcaaccct tcacttgttt acctaatgat ttgatattca aatgattagg taagcaagaa
     5941 aaaagatgag ggaattatga agacgaaaat aacagaattg ctcgatatta agtatccaat
     6001 ttttcaaggt ggtatggctt gggtagctga tggagatttg gctggtgccg tatctaatgc
     6061 cggtggtcta ggaatcatcg gcggaggaaa tgctcccaaa gaggtggtca aggcaaatat
     6121 tgacaaggtt aaatctatca cggacaaacc tttcggtgtc aatatcatgc ttttgtctcc
     6181 ttttgcggat gacattgttg acttggtcat cgaagaaggt gtgaaagttg ttaccacagg
     6241 agcaggaaat ccagggaagt atatggagcg tctccatgct gcagggatta cagttattcc
     6301 tgttgtaccg agtgtagccc ttgctaaacg catggaaaaa ttaggggtag atgctgttat
     6361 tgctgaaggt atggaagccg gaggccatat tggtaaattg accaccatga ctttggttcg
     6421 tcaggttgtt gaagccgtat ctattcctgt tattgctgct ggaggtattg cagatggtgc
     6481 aggtgcagcg gcagccttta tgttgggagc agaagcggtg caagttggaa ctcgttttgt
     6541 agtagctaca gagtccaatg cccatcaggc ttataaggaa aaagttttaa aagctaagga
     6601 tattgatacg actgtatctg cttctattgt tggacatcct gtgagagcca tcaagaataa
     6661 gttgtcatct gcttatgcag ctgcagagaa agatttcttg gcaggtaaaa tttcggcaga
     6721 tgctattgaa gaacttggtg caggagcact ccgcaatgca gttgttgatg gtgatgtaac
     6781 aaatggctcc gtaatggctg gtcaaattgc tggtctagtt tctaaagaag aatcttgtga
     6841 agatattttg aaagacatct attatggagc agccaaagtg attagagaag aggcaagccg
     6901 ttgggcttcg gttggagaat aggatgacaa agacagcttt tctttttgca ggtcaagggt
     6961 ctcagacagt ggggatggcc cgcgaccttt acgaatatta cccgatagtt agagaaacgt
     7021 ttgaccaggc tagtcagatt ctggggtatg acattcgcga cctaattgac aatcaagagg
     7081 ataaattaca tcagactcgc tatacccagc ctgctatttt gaccacttct gtggctattt
     7141 ttcgactgct ggaagaagag ggaatacaac ccgatatggt agctggtcta tccttgggag
     7201 aatattcagc tctggtggca tcgggagctt tggcctttga agatgcgatt gccctgattg
     7261 ctaagcgtgg tgaatatatg gaaacggctg cgccagcagg gactggtaag atggttgctg
     7321 ttctcaatac agatgtcaac ttgattgaag aaatctgttc gactgttacc tctgggattg
     7381 tttccccagc caactacaac acaccagccc aaatcgtcat cggaggcgag gtggctgcag
     7441 tggatgaggc ggttgaattg ctgaaggaag caggtgtcaa acggatgatt ccactcaatg
     7501 tgtcgggtcc tttccacacg gctctcctgc aaccagcgtc ggagaaattg gttcaggcct
     7561 tggagcaggt ggaatttgcg gacttccaag tagagttggt cggtaatacg gaagccaagg
     7621 ttatgaaaaa agaggacatc aagtccctct tgccccgtca agtgatggaa ccagttcgtt
     7681 tctatgagtc aatcgcaacc atgcaggaag ctggtgtgat taactttatt gaaatcggtc
     7741 caggtaaagt cttatcagga tttgtcaaga aaatcgataa gacagcgcat gttgcagcaa
     7801 ttgaagatat agaggggcta caaatagtat taaacaaatc atgaggagga atcatggaat
     7861 taacaaataa aaatgttttt gtaacaggtt caagccgagg aatcggcttg gccattgccc
     7921 ataagtttgc cagtcttggt gccaatgtgg tgctgaacgg tcgtggtcag ctaggccagg
     7981 acttgctgga tagctttgca gattacggtg tgaaagtcct agctatttca ggagatattt
     8041 caagcgcgca agatgccaaa cgaatggtgg cagaagccat cgaaacactc ggtagcgtcg
     8101 atattttggt caacaatgca gggattacca aagacggtat ggctcttcgt atgacggaag
     8161 aggattttga tacggttctg aaggtaaatc ttactggaac ttttaatatg acgcaagctg
     8221 ttttgaaacc aatgacaaag gctcgtgagg gtgctattat caatctatca agtgtatctg
     8281 gcttgattgg aaatgctgga caggccaact attctgcttc taaggcaggt gtgattggat
     8341 ttactaaagc cattgcccgt gaggttgctg ggcgaaatgt tcgtgtcaat gcgattgcgc
     8401 cgggctttat ccagtctgat atgacagatg ttttatctga caagattaaa gaagctatga
     8461 ctgcacaaat tccgatgaaa cgctttggtg cgacagaaga agtagctgat gttgctgtat
     8521 tccttgccaa gcaagaatat ctaactggac aggtgattgc ggttgacggc ggcttgacca
     8581 tgcagtaatc cagtcttcca gtttatcttt tattacgtcg acgagcgagg aaactatgtt
     8641 tccagatttc cagcttcaaa caatctattg gcttgtttga actcgcttta ccgtactaac
     8701 ggagcgttgg gagtgggaaa gaagtcgacc aagataaaaa gagttcgtct tcccaccccc
     8761 cgcacagttg attaggccag atttggagtg caaaacacga acaaatctgg cctaatcaac
     8821 cactgcgctg agatgttgac acgaaccctg agtagtggtc ctgggctttt tgcccagcct
     8881 cacgatttcc aaccttcaat agtccactgg attattgaag ctgtcagcaa cttgttgaaa
     8941 tgaacttttt cgtagtacta aaagcaaact gaaagactgc aataacgcac tgtaaattca
     9001 atgttgtttt gtgtgttatt ttaagagagg tttgaaaatg acaaaattaa atcgtgtagt
     9061 agtaacaggc tacggtctga catctccaat cggaaatacg ccagaggagt tctggaatag
     9121 tttgaaggct gggaaaattg ggatcggaaa gattaccaag tttgatacca gcgagtacag
     9181 cgtacttaat gcagcggagt taaaaggttt tccttttgat aaatactttg tcaaaaaaga
     9241 tacaaatcgc tacgataatt actcgctcta cgcactctat gcagctaaag aagcgattgc
     9301 taatgcacag ctggatacag agacagtgga tagtgaccgt tttggtgtta tcttatcaac
     9361 aggtatcggt ggtattttgg aaattgaaga gcaagttgct cgtatgaatg aaaaaggtcc
     9421 aaaacgcatt cgtcccatgg ctcttccaaa ggccctgcca aatatggcgg ctggaaatat
     9481 tgctatgcag gtcggtgcaa atggcgtctg caagtgcatc atcacagcct gcgcttcatc
     9541 aaatgatgct ttaggggaag ccttccgtga aatcaagttt ggtttccaag atgtgatgct
     9601 ggctggcgga gcagaggcag ccattactcc gtttgcaatc ggtggtttcc aagctttgac
     9661 agctatgtcg actactgagg atccagaacg tgcgtctatt ccatttgaca aggaccgcaa
     9721 tggttttgtc atgggagagg gttccgcggt tttagtattg gaaagtttgg aacacgcaga
     9781 agagcgtgga gcgacgattt tggctgaaat cgttggttat ggaaatacct gcgatgctta
     9841 ccacatgact tctccacatc cagaaggtct gggtgctatt aaggccatga agttggccat
     9901 ttcagaagca ggtttagagc cagctgatat tgactacatc aatgcccatg gtacttcgac
     9961 accggctaat gaaaaagggg aaagccaagc tatcgtatct gtcttcggca agaacacgcc
    10021 agtttcttct accaagtcct tcactggtca cttgttgggt gcagcgggtg ccgttgaagc
    10081 ggcagctgtc attgaggcta tgcgccattc ttacgcacca aagacagctg gtacgacaga
    10141 attatctgaa gatattgaag cggatgtcat ttatggacag gggcgtgata tggaaatccg
    10201 ccatgccatt tcaaatacat ttggctttgg tgggcataat tcagtcatcg ctttcaaacg
    10261 ttgggaggcc taagtgaata ttacagaaat taaggacctg atgagtcagt ttgaccagtc
    10321 aagtttgcga gaattttcgt atagcaatgc tggggagacc ttgcatttca gtaaaaacca
    10381 gcaggcgaca gtggctccga gtccagcggt agaagctccg cttgccccag taactcctgc
    10441 aagcccagcc cctgtgccag cagttgaaat gagtgagcca gctccagcag aagcaggtgc
    10501 cagccccgtg gcagaaggtg cggtagtgga aagccccttg gtcggcgtgg cttatttgtc
    10561 cccagcccct gacaagccag catttgtagc ggtcggcgat acggtcaaaa aaggccagac
    10621 cctcatgatt atcgaagcta tgaaggtcat gaatgaagtt cctgctgacc gtgacggagt
    10681 ggtcacagag attttggtgg ccaaccaaga tgttgtagaa tttggacaag gattggtacg
    10741 cattaaatga ttgatatttt gaaaattaaa gaggctcttc ctcatcgcta ccctatgctc
    10801 ttggtcgatc gtgtccttga agtatcggaa gatgagattg tcgccctcaa aaatgtgacc
    10861 attaacgagc ccttcttcaa cgggcatttc ccagactatc ctgttatgcc aggcgttctc
    10921 atcatggagg ccttggctca aaccgcaggt gtcttggagt tatccaagga agaaaacaag
    10981 ggcaagctgg ttttttatgc cggcatggac aaggtaaaat ttaagaagca agtggtacct
    11041 ggagaccagt taatcatgac agctaaattt gtcaaacgcc gtggaaccat tgcggtcgtt
    11101 gaggcaaagg cagaggtgga tggaaaactg gctgcgtcgg gggtgttgac ctttgctatc
    11161 gggaaataat atctaaaagg agataggatt catcatgttt gagaagattt tgattgccaa
    11221 tcgtggtgag attgcggtgc ggattattcg tgcggccagg gaattaggga ttgcgacggt
    11281 ggctgtctat tcagaggctg acaaggaagc tctccacacc atgttggcag atgaggctgt
    11341 ctgtatcggc ccgacccgtt cgacagattc ctatctcaat atgcaggcag tcatctcggc
    11401 ggctgttgtg acaggtgccc aggccattca tcctggcttt ggatttttga gtgaaaactc
    11461 caaattcgcc accctctgtg aagaggtcgg catcaagttt atcgggcctt ctgggacggt
    11521 catggatacc atgggcgata aaattaatgc ccgagcagag atgattaagg ctcaggtgcc
    11581 tgttattcct gggtcagacg gtgaagtctt aaccagccaa gaagcccttg aagtcgctga
    11641 aaagattggc taccctatca tgctcaaagc atcggcagga ggtggcggta aggggattcg
    11701 taaggtggaa aaagctgaag acctagtgcc tgcctttgaa tcagcttcca gcgaagccaa
    11761 ggcagccttt ggcgacggag ccatgtatat ggagcgtgtc gtctatccag cccgccacat
    11821 cgaagtacag attttggcgg accagcacgg tcatgtcatt catctgggag aacgagattg
    11881 ttcccttcaa cgcaacaacc agaaggtctt ggaagaagct ccgtctatcg ccattggtca
    11941 gactatgcgg gaacgtatcg gtcaagcagc agtgcgggct gcccagtcag ttggatatga
    12001 aaatgcagga accattgagt ttcttttgga cgaagccaag ggcgaatttt acttcatgga
    12061 aatgaacaca cgggtgcagg tggaacatcc tgttaccgaa tttgtcaccg gtgtggacat
    12121 tgtcaaggag caaatcaaga ttgcggctgg tcaggaactc agtgtccgcc aagaagatgt
    12181 gaagattaca ggccatgcca ttgaatgccg catcaatgct gaaaatccag ccttcaactt
    12241 cgccccaagt ccaggtaaga tttccaatct ctacctgcca agtggtggcg ttggtttgcg
    12301 tgttgattca gctgtttatc caggctacac cattccgcct tattatgatt ctatgattgc
    12361 caaggtcatc gtgcatgggg aaaatcgttt tgaagccctg atgaagatgc agcgagccct
    12421 ctacgaattg gaaattgacg gagtggtgac caatacagac ttccagctgg acttgatttc
    12481 cgacaagcga gtggtggctg gtgattatga tactgcctac ctcatggaag aatttttacc
    12541 ccgctatcaa gaagaattaa aaaagtaaac taggagaatg ctatggcttt gtttcgcaaa
    12601 aaggacaaat atatccgcat caatcccaat cgttcgcgga tagagtcggc acctcaagca
    12661 aagccagagg tgccagatga actcttttcc aaatgtcctg cttgtaagga aatcctctac
    12721 aagaaggact tgggaccaga gaaaacctgt caacactgtt cctataattt tcggattaca
    12781 gcccaggaac gcctggcttt gacagttgat gaaggctcgt ttgaggaatt gtttacaggg
    12841 attgaaacca caaatccgct ggacttcccc aattatttag aaaaattggc agctacccgt
    12901 caaaaaacag gtttggacga agcagtgttg acaggcaagg cgacaatcgg cggacagcca
    12961 gttgctttgg gaatcatgga ttctcacttt atcatggcct ctatgggaac ggtagttggt
    13021 gagaaaatta cccgcctctt tgagttggcg attgaagagc gtttgccagt tgtcctcttt
    13081 accgcatctg gtggtgctcg tatgcaggaa ggcattatga gcctgatgca aatggccaag
    13141 atttcggcag ctgtaaaacg ccattctaac gctggcctct tttacctgac ggtcttgaca
    13201 gaccccacaa caggaggtgt gacagctagt tttgccatgg aaggcgatat tatcctggct
    13261 gagccacaga ccttagttgg ttttgcagga cgacgggtta ttgaatcaac cgttcgggaa
    13321 aatctgcctg acgatttcca aaaggctgaa tttttacaag aacacggctt tgtcgatgcc
    13381 attgtcaaac gccaggactt gccagcgacc atcagtcgct tgttgagaat gcatggaggt
    13441 gtcagatgac cagtgatgta gctcgtatga tccgtttggc acgtgaccaa gctcgcctga
    13501 cgaccttgga ctatgccaca caactctttg atgactttat cgaactgcac ggcgatcgga
    13561 atttccgtga tgacgaagct gtagtgggtg gcatcggctt tctagcaggt cagccggtga
    13621 cagtcatcgg tatccaaaaa gggaagagtc tccaagacaa cctcaaacgg aatttcggtc
    13681 agccccaccc tgagggctac cgcaaggtcc ttcgccttat gaagcaggcg gaaaagtttg
    13741 gtcgtcccgt tgtcactttt atcaataccg ctggagccta tccaggcgtc ggtgccgagg
    13801 agcgtgggca gggcgaggcc attgcccgca acctcatgga gatgagtgac ctcaaggtgc
    13861 ccattatcgc cattatcatc ggcgagggag gatctggtgg agctctggct ctagcagtcg
    13921 cagaccaggt ctggatgctg gaaaactcca tgtatgccgt cctcagccct gaaggcttcg
    13981 cctctatcct ctggaaagac ggcagtcggg ccatggaagc ggcagaactc atgaagatca
    14041 cgtcatatga gttggagaaa ctacaagtgg tcgatcgggt tgttttggaa aatggcagag
    14101 cgaccaagga agtcctggct gacatcaagg aaaacctcat tagccagttg gctcaactac
    14161 aagccttgcc acttgatgag ctactcgaaa accgctatca acgctttaga aaatactagg
    14221 aagacctagt atttttttga tagatttgat acaatggata aaataatttc aggaggtttt
    14281 ccatgttagt aaaagcagat ctatcaaacg tagaagaatt attgcctatt cagcaccgag
    14341 catttgcagc tttgtatgaa acctatcagg accagtacaa ccctgccatt gaaactatgg
    14401 actatttcca gtcacacttt gcacgaccaa attgcaccta ctacaagatt gttgaggaag
    14461 ggcacacagt cggtttagtg cgaacaacgg tcgctgaaga tgctgaccag ggctggctag
    14521 gtctgattgg cattgaccca gaccacagag gaaaagccta tgggcacaag gctatgctcg
    14581 aattggaaag cctttatccg acagttagac gctgggtgct ctgcacggtt ttgcaggagt
    14641 ctaaattagt ttctttctac gaaaagctcg gctacaaggc catcaaaaca gagcctgaac
    14701 aagaaggcat ggatatggtc tatatggaga agtggatagg agattcagat gcttgaacag
    14761 tgtcgcagat acctggatta tcaaggagtt cattattgca agcctgaaaa agcaggagct
    14821 ttggcaggcg atatgatagc cttaagaaat gtagcacagg cagcacgcaa ggagtttcag
    14881 acattaagtt tagtcttagc tgagcacgtc agtccctttg tgcctgagag ggtcagtcaa
    14941 tggatgaatc aagcccaaat ttgcagacca catttttgga cctattaccg ttttccctct
    15001 gctagtctaa cggactcggc tcttgctatt cgtttgtatg gtaagggaac tgaagcgggg
    15061 atttcagtag aagtcagttt tattgaacga aagaaagtgg agacctctct gcttcagcaa
    15121 aatcgtgttc tgaactgctc aattgcggca ccgctttatt attgggtgca agagggacag
    15181 gtattttatc gcctggatgg aacggaggag aatagacaga gcctattaca agccgttgaa
    15241 gcaggacgga ttcgtaaagt attggtcaag tatgatgtac cgtttagaac cgttgcctcc
    15301 gaggaggaac tgctggaacg cttgattcag ggcttttctc tactagaacc ctacttgaag
    15361 atgacacacc ccgctgaata atcagtgggg tgatggcaac aaacatcgta agggtgtttg
    15421 ttttttagtg tatattgaca aattcattga ttttttgtaa aattgtatca taacaacaga
    15481 acagattgat tgacattgcg tgaaatccta taatagattg aaggcactct atatgatgga
    15541 gcggaagagg agaagagtat ggagaaactg agtcaaggtt tggaatggat tggtcggaag
    15601 atttggctat tattaaggat ttttggcctc ttagtgcttg ctgtccttcc agatctgatt
    15661 ttggaagggt ccaaatcgca agtcaccttg gtaatagttg ggctgatgac gcttggactt
    15721 ttggtctttt tctggtggcg ggctgaaaaa atgatctgcc agtctgggac cctaacattc
    15781 ttacttggga cggtctggcg atagttatat tagcctttgc tatcatgcag ttgacggata
    15841 tgcttggtta ttatttgttg gaattgcagg gtgttaccac aacagaaaac gatgcttcga
    15901 ttatagaatt tttgaaggga attcctttta gactggcact cttgacaaca gcatttttgc
    15961 cagcagttgt agaagaagtc atatttcgag gctacttctt taagaaatta tttggatcac
    16021 aggttttgtt aggaatagtt gtttcaagcc tcgtttttgg ctcctttcat ggtccaacag
    16081 acttaggctc ttggttgatt tatgctggca gtggtattat tttgtctctc ctctattaca
    16141 agagtagata cttaatctat ccaataatcg tacacttggt gaacaatttc attgcaacag
    16201 ttttttacta tatataagaa aagacggttc catacggatt cgtcttttgc tataagtaga
    16261 aacatggaat ttaacaaagt ccggagagtg agcgcataat taaatcaaca tcttggtttt
    16321 ccaactcacg aatgatatga agataggttt tctgggtcgt tgttatgctt gcatgaccaa
    16381 gtcgtctagc aacacttgcg atagaaaccc cggcaaataa taaaatagat gcatgggtgt
    16441 gtcgcagggc gtgaatggtg acgacaggaa gatttgcact taggcaacgc ttttcaagaa
    16501 tggcattgat ggtagagttg taaatccgtt ctttcttcac gaaaagtagg tcatcaggtt
    16561 gggtgtgctg taataagttg gcgaattggg tgctggtttg ccagtcgagt tggatttttc
    16621 gaattgaaga tttatttttt gtaggtaaaa atccgccgtc tcctttataa ttccatgttt
    16681 tatcaatggt caatagctgt cggtgaaaat caaaatcttg agtagtgaga gcaagggctt
    16741 cggagaatcg aatacctgtt ttagcaatca ataagatgag ccaatcccaa ttcagctcct
    16801 ctccgagacg taattcttgt atcaactgat gaagttcaaa ttggttgagg tattttgtct
    16861 ttttctttcg aggaagtctt cctttgataa tcaccttacg tgtcggatct cgttcaataa
    16921 ttccttcatc tacagcgtct aaaattgctc ctttcaactg gtgatgaaaa tccatggttg
    16981 tctgtcgttc atgtgtttta gcatagctgt taatcaattc ttggtaacga acccgattca
    17041 aatcttccaa caacagacta ggagctagcc tgataagcca ctgcaatgtc attctatatt
    17101 tcttgagagt gacttctcga atggctcctt ccttgtaaat ccgaagccaa tttgcataat
    17161 agtgatggaa aagctgtttt tcttttgata agttcatggc agaacctcct ttttaatttt
    17221 taaaaattac ataatttatt gacagatttc cgaggagatg ctacacttat tttagcgaaa
    17281 aaatgtaatt atgaaaaatt tcaaagaaag gaaagttatg aaactagaag aattggctct
    17341 ctttacggga ggtagtttac aggttcgctt ggatactacc agctgggaag atgctagaga
    17401 ctatatcctc tacaatcagc aacatcatca attggatggt tatgaggtga tagaagaagt
    17461 gatagatagt cggaccgtga cgacagatag agaagtgacc ttgctcgagg agggcgactt
    17521 actctttagt ttactatcgg ggaaggctgt tttagtgaga gcggagcacg caggcttact
    17581 ctatacacag aactatatca aaattgagcc cattgcaaag ctggataagg ccttcttact
    17641 gtatctgatt aatgaatcct cggctattag acggcagttt taccaaagct tacaggggag
    17701 tgaggtaatg aaatatacag tcaagcaact gaaaagttta cagctagacg ccttgcctcc
    17761 tttagatagt cagcagaaac taggaaagat ttatttagat ggattagcct tacggcagaa
    17821 gcggcaagct tatgcgcaag gagatttttt acggctaaca tatttgttaa aggaggttta
    17881 cgcacatgaa tgaattagca tttgaaacag aattgattca atatattacg acaggtacta
    17941 ttacacagcc ggagcaccta gaaggtctag aatggtctgt tagtgattct tcgaccctgt
    18001 atgcagtcaa atctaaactg tggacttatg agccgagtat caagacaacg gagcaattat
    18061 gggaaaattt tcgtaatatt ctctggcaac acaaccaaca tcgattggac aaacccttga
    18121 gcgataccga gtttgctcaa gtcaagcaga ttatttcaaa tctgaagaca ccttatgagg
    18181 ctggccagtt tttatatggg attagcggcg tttccgaatt ggaaattacg cttgatgatg
    18241 gacggcatac atttctgaca gtatttgacc aacgtaagat cggtggtggg gacactgtct
    18301 accaagtggt gaaccaaatt cgtagggctc ctaaaatccc tggacgacga gaatgtatat
    18361 tcgatacaac cttgttgatt aatggcttac ctattattca gattgaagag aaaaaggata
    18421 cggtagatgt ggacaaggca ttgaaccaaa tgcaccaata tatccaggaa cggcaatata
    18481 cggatatttt ttctaccttg cagattctag tcgctatcgc ccccaacaat gttcgctata
    18541 tggccaatac cactgcggaa aaatttaata aggactttgc cttccactgg cagcgtaaat
    18601 ctgacggtag taaggttcgg aattggaaag aatttgcgga ttccatgctc agcattccta
    18661 tggcccatca gatggcgacc aactacatga tcttagacgg gacaccgcag aaacaaatgc
    18721 tgaaagtcat gcgtccctat caagtttacg ccactcaggc ggtcattgag gggataaaaa
    18781 aagtcgattt tgaactaggc tgtaacaagg ttggctacat ctggcatacg acaggctcag
    18841 ggaagacgat cactagtttt aaaacagctt ggctggcaag tcgaatgccc aaggtagaca
    18901 aggttgtctt tctagtggat cgtatcgcat tgaccaatca gactaaggat gattatctgg
    18961 cttatgatcc agaagctggg gagaatagtt ttggcagtat tcgagaaact caaaatacca
    19021 cagccttggc acggcagttg actagtaagg atagtgggat tatcgttact tctgtgcaaa
    19081 aattggatac actggtgaag agaaaatcct tcaaatcacc agataagcat atcgtgttta
    19141 tcgttgacga agctcatcgc tcaactggtg gagatagttt tgcagggatt caaaaggcct
    19201 ttaagaaagc ggcttggatt ggctatacag gaactcccaa cttagatgat acaggtgcta
    19261 gtctgcggac gcaagatatt tttggccctc ttcttcatgc ctacactatt aaaaatgcca
    19321 ttagtgaccg caatgtacta gggtttaagg tagattttga gacaaccatc gatcaggaaa
    19381 aactaaaaac aacttactta ccagattttt atcggcaaaa atacccgaag tggacaagtg
    19441 cacagattga agaaaagatt gccaatctaa gtccggatga tatggatgat gcggtgactt
    19501 ctagcgtcta tgacgaaaat ccagagcatg ttcgattggt agtcgaagat gtcttcaaac
    19561 actgggcaaa tcgctctggc agtggccgtt acaatgcctt gttcaccacc cacgtaggtg
    19621 gaggcagggc ctccacaccc atggctatga tgtatttccg agagtttcaa cgaatcaatc
    19681 aactgcatcg agagcaagga ggtctggtct tgaaggtggg agtgaccttt gccttgaata
    19741 ctcaaaataa tgatagcatg gtcgagtcaa atactggttt gcttgaagct attacgatct
    19801 ataacacaga atttgggacg tcctttggct tgtcagatgt tgcttcttat acagaggacc
    19861 tggttagtcg cctcaataga acagccaagg acaagaacta cttagatttg gtgattgtcg
    19921 tggatcagtt gttgacaggt ttcaatgctc ctgagctcaa tactctttat gttgaccgaa
    19981 cccttaaagg agctagtctt atccaggcct attctcgtac caatcgtgtg gaggatatgg
    20041 ataaaaaacc ttttggacgg gtggtcaatt accgctggcc agctcataat gagaaattga
    20101 tgaatcaggc cttgggcatc tatgcagatg atagtgctgc caatttatca gatgcggaac
    20161 aaaaaaataa caatgaaaaa aatggactgg tggctaagcc tttcaaggaa gtatttgctg
    20221 aagtcaaaca aacggtcgac caattgagag agttgacggg tgatttcaat gatattccga
    20281 agtctgagaa aaagcgtgag gctatgttag aattgctccg tgcttacaat caaggactgt
    20341 ctaagctcaa acagtatcag ccagaggagg ttgatggggt gattgaagga tttgattaca
    20401 atcagccgga ccgctttatt gaaagtttgg gaatgtctgt agaggaagaa aaacttttga
    20461 cgacggtctt agccaacgaa ttgaaacgaa ccttggcgaa agagcaaaaa gttagcattc
    20521 accaaattga actcaagatg actcacatca aggaagtgac aattgactac gataaattga
    20581 ccgaactggt tgagctcttg ctgaatcagg ttcacgaaga gaagatggta gaagccgcag
    20641 ctaccaggga tgaattggat agatttgcaa gcggtttgga agacctcaac tatgctgacc
    20701 agatccgtca aacaggggat gcaatctatc gaggagattt tccgacaaaa gatatgaact
    20761 ttgcttatcc agtcaagggg attgatagtc agaagattat ccaagaagct tcgcatgttt
    20821 acattgacaa gaagctcttt gctttccgaa tccaatgggg cttgacggaa catatccgca
    20881 accaaaaact acgagagttg ttggcgagac atcgatatgg tcagaatgac ttggatgata
    20941 ccggacactt acgtaccttg ataaaagaag ctagtcgcga ttaccaggtc tgggcagaag
    21001 atgatgctgt taaaggcttg agcaagattc agtaccgtaa tcagttgaga caagcactat
    21061 atgatttggc cgacgacttg gttggtcaat aggttgcgat tttgttggat gatagcgtat
    21121 aatagaatgg aaatcgtccg aaaaattgca aaaataactt tcagaaatag aacaaaaaga
    21181 gagtagaaaa ggagggacta tgacaaaaga aaaatcaacc gtaccacgat tgcgcttccc
    21241 cggattcacg gacgcttgga aacagcgtaa gttgggggag gtaggttatg ctaagtcggg
    21301 tgtggggttt ccagagaagg aacaaggagg gacgacggga attccgttct ttaaagtttc
    21361 tgatatgaat actaatggaa atgaaaatga attagttgta gcaaataatt atgtaacaga
    21421 tgaacaaata agcaaaagaa aatggagggt tattacagaa gtacctgcta tcttctttgc
    21481 aaaagtcggt gctgcagtaa tgttaaatcg taagcgtttg ataaggcatc ctttcctact
    21541 tgataacaat acaatgactt atgtttttaa ccatactcaa tgggatacca attttggaaa
    21601 aacactattc gaaacaattg atttgataca actaactcag gtgggagcgt tgccgtcata
    21661 taattcttca gatgttgaaa ata
//
LOCUS       DATUCG010000023        20928 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.024, whole genome shotgun sequence.
ACCESSION   DATUCG010000023 DATUCG010000000
VERSION     DATUCG010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20928
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.024"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(<1..2467)
                     /locus_tag="VCA20_000713"
     CDS             complement(<1..2467)
                     /locus_tag="VCA20_000713"
                     /inference="COORDINATES: protein motif:HMM:TIGR04320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC10/PgrA surface exclusion domain-containing
                     protein"
                     /protein_id="HEP1834566.1"
                     /translation="MKKNKYIYGTIAATTAITLATVGASTVSADTQYDIFLNRVTGEW
                     QTVNHATGEIVKSEPNGTYSSYEEAQASLATSTVEAPVTTEAPAAETTAVEVPKTSAD
                     VKPALDAQQAVVDATAQDATNAQADADTANQDVTTAQAEVDTATQAVSDAEANAANAT
                     PENIAANQADQTANLADQAANATETDEVNAEIAAQTQTVADAQTAVDTAQAEKDAADA
                     NVAAKEADVKAAQDALSGTGLAEAQANLDNASKAVTDANANVDTATQAVEDAKKADAD
                     RDAKIKAAETEVAVKSDAVDTAKAKLTAAQDESKTTTDALNKTNDAVKTATDALANVD
                     TVTIADLTQFKADKAEGDSDFMTDSGATVIEQSSTKIGEDDKSKIVDFTNITEEQAQE
                     LALYNLQVVNAIRKQLGLPALELNTGSMKAAQDQMDKYVARNKNILTSGHLPGDYFGE
                     NGGPIALAKFGTSMTMYDVKQEIYNVVMTQAFADTPSKWGHSNNIQKQVSDFGASFGI
                     IAGELYSINVFGIYTGTPITDPNDPATLQATLAKAQAEQTEAQSASDKAKSELTKASS
                     DYAKALELKTQAEKTLADATATPLQTQVAENNLRLATIALQNAEARKADAQKAVDNFS
                     ADLATKKAALDTAKTELAQAQATATAKAEALETAKVELAKQQGTLDSLNKDKVALLAE
                     KDRLVEEAKALATELKGYLEAPAILANAQATLTEKQAALTEAQAKAETAQDKLETVTA
                     KLAAEQAKLAELQAEYNKLKDLEDKAKDNVIATLPDGTIVAVPKDAPTAVEKPAINID
                     AVKDAITKGQDVTVVDGEVVVTNP"
     gene            complement(2608..3864)
                     /locus_tag="VCA20_000714"
     CDS             complement(2608..3864)
                     /locus_tag="VCA20_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEP1834567.1"
                     /translation="MNYQETRRWLSNRPASDLENGVARVNWILERLDNPQFQVPTIHF
                     VGTNGKGSTLNALQSILQSSGYRVGRFTSPSIIDFREQIVFEQEMISEEDFARIVTDL
                     QPLIEDLDQTAGLDAISEFEIVVVAMFVYFAHYQRPDILLVEAGMGGLQDATNVLAPL
                     AVVCPSIGLDHQAFLGETHAAIARHKVAVLREGVPLIYATDLQEVVAVFEDHASQLQS
                     PTYAVGWEILLENSRAGFAVSSPLGRVEELKVLMQGRHQEVNAALAVTTAQLIKPHFP
                     KITNETIRQGLSQAIWPGRLELIRPNLMIDGAHNNESIAVLTQLLEEKYADRDIEILF
                     AAINTKPVDQMLSQLSQFGPVSVTTFDDFRAVKLEDYPAGYERVQTYQEWLEQADLDN
                     PQKLYLITGSLYFITYVRKYVLEKLF"
     gene            complement(3920..4471)
                     /locus_tag="VCA20_000715"
     CDS             complement(3920..4471)
                     /locus_tag="VCA20_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HEP1834568.1"
                     /translation="MEKKIPKLTVQLLAAIAMTLALVMIVENYFSIRISDTLQVQFTF
                     IPNTILGAIAGPVWAAVFAAISDPVFVLFSGQSMLFSFILIEAVSAFIYGWFFYQKPL
                     NIKNKADWLYVAGVVVLIQVVISFIMTPIALHFHFGTPWIALYSSRFIKAIFEIPLRI
                     VVTMLVLPSLQKIPELAKLMGIK"
     gene            complement(4786..5973)
                     /locus_tag="VCA20_000716"
     CDS             complement(4786..5973)
                     /locus_tag="VCA20_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEP1834569.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVLAAGIVERIGLNDSISTV
                     KYDGKKESEVLDIPNHTVAVKILLEDLLKHNIIAAYEEITGIGHRVVAGGEIFKESAV
                     IGPKEVEQIEELSALAPLHNPAHVAAMRAFEEVLPEVLNVAVFDTAFHTTMPKHAYLY
                     PIPQKYYIDHKVRKYGAHGTSHYYVAHEAAKVLGRPIEELKLITAHIGNGVSITANYH
                     GQSVDTSMGFTPLAGPMMGTRSGDIDPAIIPYLIANVDELKDAADVVNMLNKQSGLLG
                     VSGYSSDMRDIESGLEDKNPDAVLAYNIFIDRIKKFIGQYLAVLNGADAIVFTAGMGE
                     NAPLMRNDVVEGLSWFGIELDPEKNVFGNYGDISTAESKVRVLVIPTDEELVIAREVE
                     RLK"
     gene            complement(6023..6976)
                     /locus_tag="VCA20_000717"
     CDS             complement(6023..6976)
                     /locus_tag="VCA20_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEP1834570.1"
                     /translation="MNFEKIEQAYDLLLENVQTIQNQLGTNIYDAMIEQNAAYVADQH
                     ETDLVVNNNQTLKQLDLTKEEWRRAYQFLLIKANQTEPMQYNHQFTPDSIGFILSFLV
                     DQLVPTQKVTVLEIGSGTGNLAQTILNASQKELDYLGIEVDDLLIDLSASIADVMQAD
                     ISFAQGDAVRPQILKESQVILGDLPIGYYPDDQIASRYQVASPKEHTYAHHLLMEQSL
                     KYLEKDGFAILLAPNDLLTSPQSDLLKGWLQEQANIVAMIALPPNLFGKAAMAKSIFV
                     LQKKAARSLTPFVYPLQSLQEPEAIQKFMLNFKNWMQENAI"
     gene            complement(7033..7560)
                     /locus_tag="VCA20_000718"
     CDS             complement(7033..7560)
                     /locus_tag="VCA20_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGG"
                     /protein_id="HEP1834571.1"
                     /translation="MLLKKKVKAGILLYALLMLAVFSLLLQFYLHRQEAESQIAQVAK
                     HEATAYIMAQMVLEQVEEDLQERVAIANKEARQTDEMKEDKETASETANIKEEGEVQE
                     TTSTKPEPTTKSVDTSSTQNVESKPIEDKGAVAFQEGQVRYQLKGDQLLVTVELTSDG
                     LYTYQFPLGLSSEGG"
     gene            complement(7538..7972)
                     /locus_tag="VCA20_000719"
     CDS             complement(7538..7972)
                     /locus_tag="VCA20_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEP1834572.1"
                     /translation="MLKSKIPAFTLLECLVALVILSGSLLVFESLSKLLVQEAHYQGQ
                     TVQKEWLIFSSQLRSEWDQAELVKVENGKVYVNKGEQALAFGKSLSDDFRKTNDKGQG
                     YQPMIYQVDSAAISQENQLVRIDFSFKNGEERTFIYAFKEKS"
     gene            complement(7959..8252)
                     /locus_tag="VCA20_000720"
     CDS             complement(7959..8252)
                     /locus_tag="VCA20_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002282213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Type II secretory pathway, pseudopilin PulG"
                     /protein_id="HEP1834573.1"
                     /translation="MVAIRKQKNKAYILLESLVALATLVTICSLILSAVDAGRRRQAW
                     ELEQQEVLNLAQMAVQTGQDNLALNGVTVQVQRTADEIIVFHEGKEVLSVVKK"
     gene            complement(8224..8631)
                     /locus_tag="VCA20_000721"
     CDS             complement(8224..8631)
                     /locus_tag="VCA20_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEP1834574.1"
                     /translation="MLRRKSKGFTLFESLLTLFVISFLAVSVSGTVQTVFRSVQEEIF
                     LWEFEAIYKDSQKLAASSHQKVNLAIGGQEVTNGYQAVEVPGNVEVLEEKNIKFEENG
                     GNSSLTKIRFRLSRKTVTYQLYIGSGRYKKTEE"
     gene            complement(8612..8893)
                     /locus_tag="VCA20_000722"
     CDS             complement(8612..8893)
                     /locus_tag="VCA20_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEP1834575.1"
                     /translation="MKKLIEMKVKAFTLVEMLVVLGIISLLLLLFVPNLSKQKAAVQE
                     KGNAAVVKVVESQMELYELEHDEEATVADLQAKGYITDKQAAEYAKAKK"
     gene            complement(8895..9860)
                     /locus_tag="VCA20_000723"
     CDS             complement(8895..9860)
                     /locus_tag="VCA20_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEP1834576.1"
                     /translation="MARQRKVIELFNNLFASGFHLGEIVDFLKRSQLLADPYTQVLSD
                     GLLAGKPFSSLLADLRFSDAVVTQVALAEVHGNTSLSLSHIQSYLENVSKVRKKLIEV
                     ATYPIILLGFLFLIMLGLKNYLLPQLEEGNAATMLINHLPTMFLSLCGLSLVAVLAGM
                     VWFRKTNKIKAFSRLAALPFFGKFIQTYLTAYYAREWGSLIGQGLDLPQIVGLMQEQQ
                     SQLFREIGQDLEQSLSNGHSFHEHIKTYAFFKRELSLIVEYGQVKSKLGSELTVYAAE
                     CWEDFFSRVNRAMQLIQPLVFLFVALMVVLIYAAMLLPIYQNMEL"
     gene            complement(9844..10794)
                     /gene="tadA"
                     /locus_tag="VCA20_000724"
     CDS             complement(9844..10794)
                     /gene="tadA"
                     /locus_tag="VCA20_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HEP1834577.1"
                     /translation="MIQEKARKMIEEAVADRVSDIYLVPRGECYQVYHRIMDEREFVQ
                     DLAEEEVTAIISHFKFLAGLNVGEKRRSQQGSCDYDYGSGEISLRLSTVGDYRGKESL
                     VIRLLYDNDKELKFWFEAAERLAEEIKGRGLYLFSGPVGSGKTTLMYHLARLKFPDKQ
                     ILTIEDPVEIKQEDMLQLQLNEAIGATYDNLIKLSLRHRPDLLIIGEIRDAETARAVI
                     RASLTGATVFSTVHARSISGVYARMLELGVSPEELNNALQGIAYQRLIGGGGVVDFAK
                     GNYQNHSADQWNAQIDRLFAAGHISLRQAETEKIALGSPA"
     gene            10880..11830
                     /locus_tag="VCA20_000725"
     CDS             10880..11830
                     /locus_tag="VCA20_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEP1834578.1"
                     /translation="MKKLKKGDHIRIVSPSSSIKRVGGFEANLAAKEKLENLGFKLSF
                     SEHYFENDMFDSAPIASRVADLEAAFADESVEAILTTIGGFNCNELLSYLNFDLIAQN
                     PKIFCGYSDTTALLNAIYAKTGMETYMGPAYSSFKMEQGQDYQTEAWLKAVTQDSFDL
                     TPSPEWSSDAWYLPDAPRTFYPTEWKVYNPGQASGIAIGGNISTLNLLTGTEFAPKLD
                     KYILFLEEAEDDDYLIIARHLTALLQAYPNPQAVVFGRFPKETKMTEEILLAILDKHP
                     ILKTIPVLYDLDFAHTQPLFTIRIGGNVELDGEAFTIRFS"
     gene            complement(11864..12229)
                     /locus_tag="VCA20_000726"
     CDS             complement(11864..12229)
                     /locus_tag="VCA20_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEP1834579.1"
                     /translation="MYQVIKIYGDFEPWWFLDGWEEDVTSRAAFDRYEDALNAFQKEW
                     VRLSEDFPMKKSKNGTMVAFWDESDQHWCEECDEYLQRYHSLMLVEAREILPAGFIKQ
                     PTQPRVRPCKLKQNNYVNE"
     gene            complement(12380..16027)
                     /gene="rpoC"
                     /locus_tag="VCA20_000727"
     CDS             complement(12380..16027)
                     /gene="rpoC"
                     /locus_tag="VCA20_000727"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEP1834580.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERD
                     GLFDEVIFGPTKDWECSCGKYKRIRYKGITCDRCGVEVTRAKVRRERMGHIELKAPIS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEHKSIMTEREYRERLR
                     EYGYGSFVAKMGAEAIQDLLKQVDLPKEIAALKEELKTASGQKRIKAVRRLDVLDAFY
                     KSGNKPEWMILNILPVIPPDLRPMVQLDGGRFAASDLNELYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDIAGNVKAAKRLIE
                     RGDDRIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARILMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEDA
                     GREGEGMVFKDADEAVMAYRNGYVHLHTRVGIATDSLDKPWKDNQKHKVMMTTVGKIL
                     FNAIMPEGLPYLQEPNNANLTEGTPDKYFLEPGSDIKAAIAELPINPPFKKKNLGNII
                     AEIFKRFRTTETSALLDRLKDLGYYHSTLAGLTVGIADIPVIDNKAEIIEESHERVEQ
                     IKKQFRRGMITDDERYAAVTDEWRSAKEKLEKRLVEKQDPKNPIVMMMDSGARGNISN
                     FSQLAGMRGLMSAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREDDCGTDRGLDIRSITDGKEMIEPLEERLQGRYTKKTVKHPE
                     TGAVIIGPNQLITEDIAREIVNAGVEQVTIRSVFTCNTRHGVCRHCYGINLATGDAVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNSDITQGLPRVQEIFEARNPKGEAVI
                     TEVKGEVIAIEEDASTRTKKVFVKGKTGEGEYVVPFTARMKVEVGDQVARGAALTEGS
                     IQPKRLLEVRDVLAVETYLLSEVQKVYRSQGVEIGDKHIEVMVRQMLRKVRVMDPGDT
                     DLLMGTLMDITDFTDANAEVVIAGGIPATARPVLMGITKASLETNSFLSAASFQETTR
                     VLTDAAIRGKRDNLLGLKENVIIGKIIPAGTGMARYRNLEPQAINEVEIIEDTVAEEL
                     AAEAELEAVTE"
     gene            complement(16020..16196)
                     /locus_tag="VCA20_000728"
     CDS             complement(16020..16196)
                     /locus_tag="VCA20_000728"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834581.1"
                     /translation="MEQVGPNLRTERDKDQRSSLRLIYRKIAYKLSILLIIVQEFEVW
                     ESSLSQEKRGIIIG"
     gene            complement(16206..19778)
                     /gene="rpoB"
                     /locus_tag="VCA20_000729"
     CDS             complement(16206..19778)
                     /gene="rpoB"
                     /locus_tag="VCA20_000729"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEP1834582.1"
                     /translation="MAGHEVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFQDFLDYG
                     LKEVFEDVLPVSNFTDTMELEFVGYELKEPKYTLEEARAHDANYSAPIYVTFRLVNKE
                     TGEIKTQEVFFGEFPIMTEMGTFIINGAERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELETDSKDIAYTRIDRTRKIPFTTLVRALGFSGDDEIFDIFGDSELVR
                     NTIEKDIHKNPADSRTDEALKEIYERLRPGEPKTAESSRSLLTARFFDPRRYDLAPVG
                     RYKINKKLNLRTRLLNQTLAEHVINGETGEIVLEAGTVLSRDVLEKVEAQFDELNLVE
                     YIPNDNAVLLEPVLLQKFKIVAPKDPERVVTVIGNANPAENVRTVTPADILAEMSYFL
                     NLAEGLGRVDDIDHLGNRRIRAVGELLANQVRIGLTRMERNLRERMSVQDNEVLTPQQ
                     IINIRPVTAAIKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVRD
                     VHYTHYGRMCPIETPEGPNIGLINNLSSYGHLNKYGFIQTPYRKIDRATGTVTNEIVW
                     LTADEEDAYIVAQSTSPLDENNRFVDKIVMGRHQGNNQEFPADSADFMDVSPKQVVAV
                     ATACIPFLENDDSNRALMGANMQRQAVPLIDPKAPYVGTGMEYQAAHDSGAAIIAQHD
                     GKVVYADADKVEVRREDGSLDVYHISKFRRSNSGTAYNQRTLVKLGDIVEKGDFIADG
                     PSMENGEMALGQNPIVAYMTWEGYNFEDAVIMSERLVKDDVYTSVHLEEYESETRDTK
                     LGPEEITREIPNVGEDALRNLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERLL
                     HAIFGDKSREVRDTSLRVPHGADGVVRDVKIFTRANGDELQSGVNMLVRVYIAQKRKI
                     KVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHLG
                     MAACNLGIHIATPVFDGASSEDLWSTVKEAGMDSDAKTILYDGRTGEPFDNRVSVGVM
                     YMIKLHHMVDDKLHARSVGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVLQ
                     EILTYKSDDVTGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDNNE
                     VELRDLDEGEDDDIIHVDDLEKARAKAAADAAAAFAAEEAEGKE"
     gene            complement(20427..>20928)
                     /locus_tag="VCA20_000730"
     CDS             complement(20427..>20928)
                     /locus_tag="VCA20_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HEP1834583.1"
                     /translation="DAKLLSKLTAKGCHYQTIEKARSQIEVRDYWISHDVRWLSQRHP
                     KWEKLRGIGMTKNTIDKDGIITEEVRYFILSFKGDVQTFSQVVRGHWSVESLHWLLDV
                     VYREDRNQTLDKRAAFNLNAIRKVCLHLLQNMTFPKEELSYRRKQRYIFVHLEDYLPQ
                     LFGHRG"
BASE COUNT         5677 a         4898 c         4316 g         6037 t
ORIGIN      
        1 gtgggttcgt caccacaact tcaccgtcaa caactgttac atcttgacct tttgtaatag
       61 cgtctttgac tgcatcaatg ttgatagctg gtttttcaac agcagttggt gcatcttttg
      121 gtactgcaac aattgttccg tctggaagag ttgcaatgac attgtctttt gctttgtctt
      181 caaggtcttt gagtttgttg tactcagctt gaagttcagc tagtttagct tgttctgctg
      241 caagtttagc agtaacagtt tcaagtttgt cttgtgctgt ttcagcttta gcttgtgctt
      301 cagttagagc agcttgtttt tcagtaagtg ttgcttgagc gttagcaaga atagctggtg
      361 cttcaagata acctttcaac tcagtagcaa gtgcttttgc ttcttcaacc aaacggtctt
      421 tttctgcaag aagagcgact ttatctttgt tcaagctatc aagtgtacct tgttgcttag
      481 caagttcaac cttagctgtt tcaagagcct ctgcttttgc agtagcagtt gcttgtgctt
      541 gagcgagttc agttttagct gtatcaagtg ccgctttctt agttgccaaa tcagctgaga
      601 agttgtcaac tgctttttgt gcatcagctt tgcgagcttc agcgttttga agagcaattg
      661 ttgcaagacg taggttgttt tcagcaactt gagtttgaag cggtgttgca gtagcatcag
      721 caagagtttt ctctgcttga gtcttcaatt caagagcctt agcgtaatct gagcttgctt
      781 tagttaattc agacttagct ttgtctgatg cagattgtgc ttcagtctgt tcagcttgag
      841 ctttagcaag agtagcttga agagtagctg ggtcattagg gtcggtgatt ggggtgcctg
      901 tgtagatacc aaatacgttg attgagtaca attcaccagc aatgatacca aatgatgcac
      961 caaaatcaga tacttgtttt tggatattgt ttgagtgacc ccattttgat ggagtatcag
     1021 cgaaggcttg agtcattaca acgttgtaaa tttcttgttt aacatcatac atagtcattg
     1081 atgtgccaaa tttagctagt gcaattgggc caccgttttc gccaaagtaa tcgcctggta
     1141 aatgaccaga tgttaagata tttttgttgc gagctacgta cttgtccatt tggtcttgag
     1201 cagctttcat tgaacctgta tttagttcaa gtgcaggtaa accgagttgt ttacgaatag
     1261 catttacaac ttgtaaatta taaagtgcaa gttcttgtgc ttgttcttct gtgatatttg
     1321 tgaaatcaac aatttttgat ttatcgtctt caccgatttt agttgaagat tgttcaataa
     1381 cagttgcacc agaatcagtc ataaagtctg agtcgccttc ggctttatca gctttgaatt
     1441 gagtcaagtc tgcaattgta acagtatcaa catttgccaa agcgtctgtt gctgttttaa
     1501 cagcgtcatt tgttttattg agtgcgtcag ttgttgtttt agactcatct tgagcagctg
     1561 tcaattttgc ttttgctgta tcaacagcgt ctgattttac agcaacctca gtttcagcag
     1621 ctttgatttt tgcatcacgg tctgcgtcag ctttcttagc atcttcaacg gcttgggttg
     1681 cagtatcaac gttagcgtta gcatctgtga cagctttgct tgcattatca aggttagctt
     1741 gagcttcagc aagaccagta ccagaaaggg cgtcttgtgc tgcttttaca tcagcttctt
     1801 tagctgctac gttagcatca gctgcgtctt tttctgcttg tgctgtatca acagcagttt
     1861 gtgcgtcagc aacggtttga gtttgtgctg cgatttcagc gttaacttca tctgtttcag
     1921 tagcgtttgc agcttggtca gcaaggttag ctgtttgatc agcttggttg gctgcaatgt
     1981 tttctggtgt tgcgtttgca gcgttagctt cagcgtcaga tacagcttgt gttgcagtat
     2041 caacttctgc ttgagcagtg gtaacgtctt ggttagctgt gtctgcatca gcttgagcgt
     2101 tagttgcatc ttgagcagta gcatcaacaa cagcttgttg tgcatcaaga gctggtttaa
     2161 catcagctga tgttttaggt acttcgacag ctgtagtttc cgcggctggt gcttcagttg
     2221 taactggagc ctcaacagtt gatgttgcca atgatgcttg agcttcttca taagaagagt
     2281 aagttccatt tggttcagat tttacaattt cgcctgtggc gtggttgaca gtttgccact
     2341 caccagtcac acggtttaag aaaatatcat attgtgtgtc tgctgatacg gtagacgcac
     2401 caacggttgc aagggtgatg gcagtagttg cggcgattgt gccatatata tacttgttct
     2461 ttttcattat ttcctcctgt ttaggctaat aggaagggct tggccgaagt gtcaagccct
     2521 gctatactag tatagcaggc attttgtcaa gttttttgca tgatttttga gaagtttgtc
     2581 aaaacatttt ttagaaaaat tttaatatca gaataacttt tctaaaacat acttcctcac
     2641 ataggtaatg aaatatagcg agcctgtaat caggtagagt ttttggggat tgtccaagtc
     2701 cgcctgctcc aaccattcct gataggtttg gacccgctca taacctgctg gatagtcctc
     2761 cagcttcacc gcccgaaaat catcaaaagt cgtaacgcta acaggtccaa attggctaag
     2821 ctgggacaac atctggtcca caggcttggt attgatggcc gcaaagagaa tttcaatatc
     2881 tctgtcagca tacttttctt ccaagagttg tgtcaggacg gcgatacttt cattattgtg
     2941 ggcaccgtca atcatgagat taggtctaat caactctaag cggcccggcc agatggcttg
     3001 ggacaagccc tggcggatgg tttcattggt aatctttgga aaatgaggtt taatgagctg
     3061 agctgttgtc actgccaagg ctgcattgac ctcctggtga cggccctgca tcaagacttt
     3121 caactcttcc acacgaccga gagggcttga aactgcaaag cctgctctgc tattttccaa
     3181 aagaatctcc caccccaccg cataggtcgg actctgaagc tggctggcgt gatcctcaaa
     3241 tactgccacc acttcttgca aatcggtcgc atagatgagc ggaaccccct cacgcaagac
     3301 ggcgaccttg tgacgggcta tggctgcgtg agtctccccc agaaatgcct gatggtccaa
     3361 gccgatggag gggcaaacta ctgccaaggg ggcaaggaca ttggtcgcat cctgcaaacc
     3421 gcccatgcct gcctcaacca agagaatgtc gggacgctgg tagtgggcaa agtagacaaa
     3481 catagcaact actacaatct caaactccga gatagcatcc agtccagccg tctggtccaa
     3541 gtcctcaatc aagggttgca agtctgtcac aatcctcgca aaatcctcct ccgaaatcat
     3601 ctcttgctca aagacaatct gctctcgaaa atcaatgata gacggtgaag taaagcgacc
     3661 aactctgtag cccgaagact gcaagataga ctgtaaggcg ttgagggtcg agcccttgcc
     3721 atttgtacca acaaagtgaa tagtcggcac ttgaaactgg ggattgtcca agcgttccaa
     3781 gatccagttg acacgtgcaa cgccgttttc taaatctgat gcagggcgat tcgatagcca
     3841 ccggcgagtt tcttgataat tcatgattcc ctctctacaa aagcagcaac agggggatga
     3901 cctgttgctt gatactgttt tatttaatgc ccattaactt ggccaattca ggtatttttt
     3961 gtaaacttgg caagacaagc atagtcacga caatgcgtaa tggaatttca aaaatagctt
     4021 ttatgaaacg actgctatac agggcaatcc aaggtgttcc aaaatggaaa tggagggcaa
     4081 tcggtgtcat gataaaggaa atcacaacct gaatcaagac aactacacca gccacataga
     4141 gccaatcagc tttgttttta atattcaacg gcttctgata gaagaaccag ccatagataa
     4201 atgccgatac cgcctcaatc aagataaaac taaaaagcat gctctgtcca ctaaacaaga
     4261 caaagactgg gtctgaaata gccgcaaaga cagctgccca aactggaccc gcaatagctc
     4321 ccaaaatagt attgggaatg aaggtaaact gaacctgtaa agtatcagaa atccgaatag
     4381 agaaatagtt ctccacaatc atgaccaagg caagggtcat cgcaattgca gccaacaact
     4441 gcaccgttag ttttggaatt ttcttttcca taaaaaatgc tcctttttca aaaaatggag
     4501 ctgtcgtgtg tattcgacag cggatgcaat ggtttaccgc gctactctca gccaggacca
     4561 atgttcctac tctaggagac atcagatgca agcaaagatc agcttcgtta tgtggcaaca
     4621 tcccatccac atacacttga cgcaaaccat ctactctgtg caaatttctt tgctagtcta
     4681 ttgtatcaca cactatatat tttgaccact tgctaaaaac tatacctttc taaaggtata
     4741 aactataaga gaaactggca gtctccgact accagttagt ttttcttatt tcaagcgttc
     4801 cacttcacgc gcaataacca attcttcatc cgtcggaata accaagacac gaacttttga
     4861 ttctgccgtt gaaatgtcac catagttgcc gaatacattt ttttctgggt ccaactcaat
     4921 accaaaccaa gacaaacctt ctactacatc attgcgcata agcggtgcat tttcacccat
     4981 acctgccgtg aagacaatag catctgcccc atttaaaact gcaagatact gaccgataaa
     5041 tttcttaata cggtcgataa aaatgttata agccaaaaca gcatctgggt tcttatcttc
     5101 caaacctgat tcaatgtcac gcatatcact tgaataaccc gatacaccaa gcaaacctga
     5161 ttgtttattc agcatattaa caacatccgc agcgtccttc aactcatcaa catttgcaat
     5221 caagtaagga ataatcgctg gatcaatatc tcccgaacga gtacccatca ttggaccagc
     5281 tagaggagtg aagcccatag aagtgtcaac tgattgaccg tggtagttgg cagtgatgga
     5341 tacaccgttt ccgatgtgag ccgtaatcaa ttttaattcc tcaatcggac gtcccaatac
     5401 ttttgctgcc tcatgcgcta cataatagtg actagttccg tgcgctccat acttacgaac
     5461 cttgtggtcg atatagtact tttgtggaat cggataaaga taggcatgct ttggcatcgt
     5521 agtgtgaaag gctgtatcaa aaacagctac attcaaaact tctggtagta cttcctcaaa
     5581 tgcacgcatt gctgctacgt gagcagggtt atgaagcggt gccaaagcac ttagttcttc
     5641 aatttgctca acctcttttg gcccaataac agctgattct ttgaaaatct ctccacctgc
     5701 aacaacacgg tgtccgatac cagtaatttc ctcgtaagca gcaatgatgt tgtgtttaag
     5761 caagtcttct aggagaatct taaccgcaac cgtatgatta ggaatatcta aaacttcact
     5821 ttctttcttt ccatcatact taactgttga gatggaatcg ttcaaaccga tgcgctcaac
     5881 aatccctgcc gctaatactg tttcttctgg catttgataa agttgccatt ttagacttga
     5941 actacctgca ttaattgcaa ttgtttttga catatcttac ctcttttaaa acgttttcct
     6001 aactagtata acagattttc acttaaattg cattctcttg catccaattt ttgaaattga
     6061 gcatgaactt ctgaatagct tctggttctt gaagactttg taagggataa acaaacggcg
     6121 tcaacgatct tgcagctttc ttttgcaaga caaaaataga cttggccata gcagccttcc
     6181 caaagagatt tggtggcagg gcaatcatgg caacaatatt ggcttgctcc tgtaaccaac
     6241 ctttcagcaa atcgctttgc ggactagtca ataaatcatt tggagccaac aaaatcgcaa
     6301 agccatcttt ttccagatat ttgagggatt gttccatgag taaatgatgg gcgtaggtat
     6361 gttcctttgg actggcgacc tgataacggc tagcaatctg gtcatctgga tagtagccaa
     6421 taggcaaatc tcccaaaatt acttgacttt ccttcaaaat ctgcggacgt accgcatctc
     6481 cctgagcaaa agaaatatct gcctgcatga catccgcaat acttgccgac aaatcaatca
     6541 agaggtcgtc cacttcgatt cccaagtaat ccaattcttt ctgactggcg ttgagaatgg
     6601 tctgagctag attgcctgtc cccgaaccaa tttccagaac cgtcaccttt tgagtcggca
     6661 ccaattggtc tactagaaaa gataggataa atccgataga gtctggtgtg aactggtgat
     6721 tgtactgcat gggttcagtc tgattggcct tgatgagcaa gaattggtag gcacgacgcc
     6781 attcttcctt ggttaaatct agttgtttca aggtctgatt gttattgaca acaaggtccg
     6841 tctcatgttg atcagctacg taagcagcat tttgctcaat catggcatca tagatattgg
     6901 tacctagctg gttttggata gtctgtacgt tttctaatag caggtcgtaa gcctgttcga
     6961 tcttttcaaa attcatactc ctatcttatc aaaatttcac agactttgca ccaaaaagga
     7021 gagcgagtca actcaccctc cttctgatga tagccccaaa ggaaattgat aggtatacag
     7081 accatcactt gttaactcaa ctgtaaccaa tagttggtct cctttaagtt gataacgaac
     7141 ctgaccctcc tgaaaagcga ccgctccctt atcttctatt ggtttgcttt ccacgttttg
     7201 agttgatgat gtgtcaactg attttgtagt tggttctggc ttagtgcttg ttgtttcttg
     7261 tacctcccct tcttccttaa tattagccgt ttcactagct gtttctttat cttccttcat
     7321 ttcatctgtt tgacgagcct ccttattggc aatcgctaca cgctcctgca agtcttcttc
     7381 aacttgttcc aagaccatct gagccatgat gtaggcggta gcttcatgct tagcaacttg
     7441 agctatttga ctttcagcct cttggcggtg gagataaaac tgaagcagca gactaaaaac
     7501 agccagcatc aaaagggcat agagcaggat acccgcctta acttttttct ttaaaagcat
     7561 agataaaggt ccgctcctct ccgtttttaa agctaaagtc gatgcgcacc agctggtttt
     7621 cttgggaaat agctgcgctg tccacctgat aaatcatagg ctggtagccc tgtcccttat
     7681 cattggtttt ccgaaaatca tccgacagtg acttgccaaa ggccaaagcc tgttcgcctt
     7741 tgttgacata gaccttgcca ttttcaacct tgaccaattc agcctggtcc cactccgacc
     7801 gcaactgact agaaaatatc agccactcct tttgaacggt ctgaccttga taatgtgcct
     7861 cttgaaccaa cagttttgac aagctttcaa agaccagaag acttcctgat aggatgacta
     7921 aggctaccaa gcattccaag agagtaaaag caggaatttt actttttaac aactgataga
     7981 acctcctttc cctcgtgaaa aacaataatc tcatccgcag ttcgttgaac ctgaactgta
     8041 acaccgttta aggccaggtt atcttgtccg gtctgaacag ccatctgtgc tagattgaga
     8101 acctcctgtt gttccaattc ccaagcctgt cgcctgcgac cagcatccac cgctgacaaa
     8161 atcaagctac aaatggtcac tagggtcgca agggcaacca gactttctag caagatataa
     8221 gccttattct tctgttttct tatagcgacc actccctata tataattgat atgtgactgt
     8281 ctttcgacta agccgaaaac gaattttggt cagggaagaa tttccaccat tttcttcaaa
     8341 tttgatattt ttctcctcca agacctctac atttccgggt acctctacag cctgataacc
     8401 atttgtaacc tcctgtccac cgatagcaag gttcactttt tggtgggaac tggctgccaa
     8461 tttctgactg tctttataga tggcctcaaa ttcccagaga aaaatttctt cctgaacaga
     8521 cctaaagacc gtttgtactg ttcctgatac ggaaacagct aaaaaactga taacaaacaa
     8581 ggtcagcagg ctttcaaaca gggtgaagcc cttacttttt cgccttagca tactctgccg
     8641 cttgtttatc agtaatatag cctttcgcct gcaaatctgc caccgttgct tcctcgtcat
     8701 gttccaattc atagagctcc atctggctct ctactacttt gacaacggca gcatttcctt
     8761 tctcttgaac agctgctttt tgcttgctca aattaggcac aaagaggagc aggaggagac
     8821 taataatgcc caaaacgact aacatttcca ccagagtgaa cgcttttacc ttcatttcaa
     8881 ttaatttttt cattttataa ctccatattt tgataaatcg gcagcaacat ggctgcgtag
     8941 atgagaacga ccattaaggc cacaaagaga aagaccagcg gttgaatcag ctgcatggct
     9001 ctattgaccc gagagaaaaa atcctcccaa cactctgccg catagactgt caactcgctc
     9061 cccaacttgg acttgacctg accatactcc acaatcaaac tcagctcccg cttaaaaaag
     9121 gcgtaggtct taatgtgttc gtgaaagcta tgaccattgg aaagcgactg ctccaggtcc
     9181 tggccaatct cccgaaagag ctgcgattgc tgctcctgca tcaagcccac aatctgtggc
     9241 aggtccaagc cttgcccaat caaactcccc cactccctgg cgtaataggc cgtcaggtag
     9301 gtttggatga attttccgaa aaatggcaga gctgccaagc gggaaaacgc cttgattttg
     9361 ttggttttgc gaaaccaaac cataccagcc aagaccgcca ccaaactaag tccacagagg
     9421 gataaaaaca tagtcggcag atggttaatt agcatggtcg cagcattgcc ttcctccaac
     9481 tggggcagaa gatagttttt caagcctagc ataatcaaga acagaaaacc cagtaagata
     9541 atcggatagg tcgccacctc aatcagcttt ttacgaacct tgctgacatt ttccagatag
     9601 gactggatat ggctcaaact caggctggta ttaccatgaa cttctgccag agccacctgt
     9661 gtgaccaccg catctgaaaa ccgcaaatct gccagtaaac tcgaaaaggg tttgcctgcc
     9721 agcaacccgt ctgacaagac ctgggtatag ggatctgcca gaagctgact gcgtttgagg
     9781 aaatcaacaa tctcccccag atgaaaacca ctagcaaaaa gattattgaa aagctcaatg
     9841 accttacgct ggcgagccaa gggcaatttt ttctgtttct gcctgccgaa gactgatatg
     9901 tcctgctgca aaaaggcgat caatttgcgc attccactgg tctgcggaat ggttttggta
     9961 atttcccttt gcaaaatcta ccacacctcc tcccccgatt aaacgttgat aggcaatacc
    10021 ttgaagggca ttatttagct cttcaggact gacccccagt tccaacatac gagcatagac
    10081 acccgaaata gaccttgcgt gaaccgttga gaaaacggta gctcccgtca ggctggctcg
    10141 aatgactgct cgagctgttt ccgcatcccg aatttcaccg atgatgagca ggtccggtcg
    10201 atgacgcagg gacagtttaa tcagattgtc gtaggtggct ccgatggctt cattgagctg
    10261 gagttgcagc atatcctcct gcttaatctc gacaggatct tcaatggtca aaatctgttt
    10321 gtctgggaat ttcagcctgg caagatggta cataagggtg gtcttaccag agccgaccgg
    10381 acccgaaaaa aggtagagcc ctcgtccctt gatttcttct gcaagccgct cggccgcctc
    10441 aaaccagaac ttgagttcct tgtcattgtc atagagcagg cggataacca gactttcctt
    10501 gccacgataa tctccgacag ttgataagcg aagtgaaatc tctccgctcc cataatcata
    10561 gtcacaggaa ccctgctgac tacggcgttt ttcaccaaca tttaaacctg ctaaaaactt
    10621 gaaatggctg atgatggctg ttacttcctc ctcagccaag tcttgcacaa attcccgctc
    10681 gtccatgata cgatgataga cttggtaaca ctcacctcga ggaactagat agatgtcact
    10741 gaccctatct gccaccgcct cttcaatcat ctttcttgct ttttcttgaa tcatacaacc
    10801 tcctcaccct atcaattcgt aaggagactt gattttccat acttttttgt taaaataata
    10861 aaaaagcttg gaggttccta tgaaaaaatt aaaaaaaggc gaccatattc gcattgtcag
    10921 cccgtcatct tctatcaaaa gagttggtgg ttttgaggcc aatcttgccg ccaaggaaaa
    10981 actagaaaat ctgggcttca aactctcttt ctcagaacat tattttgaaa atgatatgtt
    11041 cgattcggct ccgattgcta gtcgagttgc ggacttggaa gcagcctttg cggatgagtc
    11101 ggtcgaggcc attttgacaa ccattggtgg tttcaactgc aatgaactct tgtcctatct
    11161 gaattttgat ttgattgcac aaaatccgaa aattttctgc ggttattcgg acacgactgc
    11221 cctgctcaat gccatctatg ccaagacggg tatggaaacc tacatgggtc cagcttattc
    11281 cagctttaaa atggagcaag gccaagacta tcaaacagaa gcctggctca aggctgtgac
    11341 acaagactcc tttgacctga ctccgagtcc tgaatggtct agcgacgctt ggtacctacc
    11401 agacgcccct cgcacttttt acccaacaga gtggaaagtc tacaatccag gccaggcctc
    11461 tggtattgcc atcggtggaa acatctcaac cctcaacctg ctgactggga cggagtttgc
    11521 tcccaaactg gacaagtata tactgtttct ggaggaggcg gaagatgatg attacctgat
    11581 tatcgctcgt catttaacag cccttttgca agcctatccc aatccgcaag cggtcgtctt
    11641 cggtcgcttt ccaaaagaga ccaagatgac agaggagatt ttgttagcga ttttggacaa
    11701 gcaccctatt ctcaaaacca tccctgttct ctacgatttg gactttgccc atacccagcc
    11761 actctttacc attcggattg gcgggaatgt tgagcttgat ggggaagcat ttactatcag
    11821 attcagctaa aaaggattga cccgcaaggt caatcctctt ttattattca tttacataat
    11881 tattttgttt taatttgcag gggcgtacac gaggttgcgt aggttgctta ataaaaccag
    11941 ccggtaaaat ttctctcgct tctaccaaca tcaacgaatg ataccgttgt aagtattcat
    12001 cgcattcctc acaccaatgc tggtctgatt catcccaaaa agccaccatc gttccattct
    12061 tacttttctt cattggaaaa tcctctgata gccgtaccca ctctttttga aaggcattca
    12121 gtgcatcctc atagcgatca aaagcagccc tactggtaac atcctcttcc caaccatcta
    12181 agaaccacca gggttcaaaa tctccatata ttttaataac ttgatacata atattacttc
    12241 ctttttcgta cacattatat cgaaattgta tgatttaaga aaacattctg cttgttaatt
    12301 ccttatcaag tcatatatat ggtttctttc cgaatatcaa aaagcacatc cctcaaagag
    12361 agagctgtgc catcttaatt tattcagtta cagcttcaag ctctgcttct gcagcaagct
    12421 cttctgctac tgtgtcttca atgatttcaa cttcattgat ggcttgtggt tcaagattac
    12481 ggtagcgagc catacctgta cctgctggga taatcttacc gatgataaca ttctctttaa
    12541 gaccgagaag gttatcacgt tttccacgaa tagctgcatc tgtaaggaca cgtgttgttt
    12601 cctggaagga tgcggcagac aagaatgagt tggtttcaag agacgctttt gtgataccca
    12661 taagaactgg acgagcggtt gccggaatac cacctgcaat aaccacttcc gcattggcat
    12721 ctgtaaagtc tgtgatatcc atgagggtac ccatgagaag atctgtgtcc cctggatcca
    12781 tgacacgaac tttacgaagc atttgacgaa ccattacctc gatgtgcttg tcgccgattt
    12841 ctacaccctg gctacggtaa actttttgaa cttcagaaag aagataagtt tcaaccgcca
    12901 agacatcacg gacttcaagc aagcgttttg gttggataga accttcggta agggctgctc
    12961 cacgcgcaac ttggtcacca acctcaacct tcatacgggc tgtaaatgga accacatatt
    13021 cgccttcgcc agtttttcct ttaacaaaga ctttcttggt acgagtagaa gcatcttctt
    13081 caatagcgat aacttcacct ttaacctcag tgataaccgc ttcccctttc ggattacggg
    13141 cttcaaagat ctcttggaca cgaggaagac cctgcgtaat atcgctgttt gaggctacac
    13201 cacccgtgtg gaaagtacgc atcgtaagct gtgtaccagg ctcaccgatc gattgggcag
    13261 cgattgtacc aactgcttca cccacttcaa ccgcatcacc tgttgccaag ttgataccat
    13321 aacaatgacg gcagacaccg tgacgagtgt tacatgtaaa tacgctacgg atggttactt
    13381 gttcaacacc tgcattgaca atttcacggg caatatcttc ggtaatcaat tggtttggac
    13441 caatgataac ggcacccgtt tcaggatgtt taacagtttt cttagtgtaa cgaccttgca
    13501 aacgctcttc aagtggctcg atcatttcct tgccatctgt gattgaacgg atgtcaagac
    13561 cacggtctgt tccacagtca tcttcacgga taataacgtc ttgggcaacg tcaaccaaac
    13621 gacgagtcaa gtaacctgag tcggctgtct tcaaggccgt atccgtcata cccttacggg
    13681 caccgtgagt tgagaagaac atttccaata ccgaaagacc ttcacggaag ttagacaaga
    13741 ttggcaattc catgatacgt ccgttcggag ctgacatcag accacgcata ccggccaact
    13801 gggagaagtt ggagatgtta ccacgggcac cagagtccat catcataacg attgggttct
    13861 taggatcttg tttttcaacc aggcgttttt ccaatttttc cttagctgaa cgccattcat
    13921 ctgtaacagc tgcataacgc tcatcgtcag taatcatacc acgacggaat tgtttcttga
    13981 tttgttctac acgttcgtga gattcttcaa tgatttcagc cttgttgtcg ataactggga
    14041 tatcggcaat acccactgtc aaaccagcaa gtgttgagtg atagtaaccc aagtctttca
    14101 aacggtcaag aagggctgat gtttcagttg tacggaaacg cttgaagatt tcagcgatga
    14161 tgttaccgag atttttcttc ttgaatggtg ggttgattgg caattctgca atggcagcct
    14221 tgatatctga acctggttcc aagaagtatt tatcaggagt tccttctgtc aagttagcat
    14281 tgtttggctc ttgcaagtat ggaagacctt ctggcataat tgcgttgaac aagatttttc
    14341 cgacagttgt catcatgacc ttgtgttttt ggttgtcttt ccaaggttta tcgaggctat
    14401 ccgttgcgat acctacacga gtatgcaagt gaacatagcc attgcgataa gccataacag
    14461 cctcatccgc atccttaaag accatacctt caccttcacg accagcatct tccatggtca
    14521 agtagtagtt acccaaaacc atatcctgag atggtgttac gactggttta ccatctttag
    14581 ggttaaggat gtgttctgcc gcaagcataa ggatacgtgc ttctgcttgt gcttcttctg
    14641 acaatggaac gtgaatcgcc atctggtcac cgtcaaagtc ggcgttgtag gcttcacaga
    14701 caagcgggtg caaacgaagg gctttaccgt cgatcagaac tggctcaaaa gcttggatac
    14761 ccaaacggtg aagagtaggt gcgcggttca aaagaactgg gtgttctttg atcacttctt
    14821 ccaagatatc ccagatacga tcatccccac gttcaatcaa acgttttgca gcttttacgt
    14881 taccagcaat atcacgggca acgatttcgc gcatgacaaa cggtttgaag agctcgatag
    14941 ccatttcgcg tggcacacca cattgataca ttttaagcgt tggaccaacg gcgataacgg
    15001 aacgccccga gaagtctaca cgcttaccaa gcaagttttg acggaaacgt ccttgcttac
    15061 ctttaagcat gtggctgagt gatttaagcg gacggctacc tggtccagtg attggacgac
    15121 cacggcgacc gttgtcaatc aaagcatcta cagcttcttg gagcatccgt ttttcgtttt
    15181 gtacgatgat ccctggagcg ttaagttcca atagacgagc caaacggttg ttacggttga
    15241 taacacggcg gtagagttcg ttcaaatcag atgcggcaaa acggccacca tccaactgaa
    15301 ccatcggacg caaatctggt ggaataactg gaaggatgtt gagaatcatc cactcaggct
    15361 tgttaccaga tttgtagaag gcatccaata catccaagcg acgaactgct ttaatgcgtt
    15421 tttgaccaga agctgttttc aattcttctt tcaaagctgc gatttctttt ggaagatcga
    15481 cttgtttcaa gaggtcttgg atggcttctg cacccatttt ggcaacgaat gaaccataac
    15541 catattcacg caaacgctca cggtattcgc gctcagtcat gattgacttg tgctcaagcg
    15601 gtgtatcttt cggatcgatc accacgtaag ctgcgaagta gataacttct tccaaggcac
    15661 gtgggctcat atccaaggtc aagcccatac gacttggaat ccctttgaaa taccaaatgt
    15721 gtgaaattgg tgctttcaac tcaatgtgcc ccatacgttc gcgacgaact ttggcacgag
    15781 tcacttcaac accacagcgg tcacaagtaa tccctttata acggatacgc ttgtatttac
    15841 cacatgaaca ctcccagtct tttgttggac caaagatgac ttcgtcaaag agtccatcac
    15901 gttctggttt aagtgtccga tagttgattg tttcaggttt tttaacctca ccgtaagacc
    15961 atgaacgaac cttacttggt gaagctaacg tgatttgcat acttttaaat cgatttacgt
    16021 caaccaataa taatccctct cttttcttgt gagaggctgg attcccagac ctcaaattct
    16081 tgaacaataa tcagcagaat ggatagcttg taagctattt ttctataaat cagacggagg
    16141 gaagaccgtt ggtccttatc tctttcagtt ctgagatttg gaccaacttg ttccatccat
    16201 tctaattatt ctttgccttc tgcttcttct gctgcaaagg ctgcggctgc gtcagctgcg
    16261 gcttttgcac gtgctttttc aagatcatct acgtggatga tgtcatcatc ttcaccttca
    16321 tcaaggtcac gcaattctac ttcattgttg tcttcatcaa ggacacgcat atccaaacca
    16381 agtgattgca attctttgac aagaacgcgg aatgattctg gaacacctgg ttttggaatt
    16441 ggtttacctt tggtgatggc ttcataggct ttcagacggc ctgtcacatc atctgacttg
    16501 taagtcaaga tttcttgaag gacgttagaa gcaccgtagg cttcaagggc ccaaacctcc
    16561 atctcaccga aacgctgacc accaaactga gccttacctc cgagtggctg ttgggtaacg
    16621 agtgagtatg gtccgactga gcgggcgtgg agtttatcat caaccatgtg gtgaagcttg
    16681 atcatgtaca tgacgccgac agacacacgg ttgtcaaatg gctcacctgt acgtccatcg
    16741 taaagaatgg ttttggcatc tgaatccata cctgcttctt taacagttga ccagaggtct
    16801 tctgaacttg caccatcgaa aactggtgtt gcgatatgga tgcccaagtt gcaagccgcc
    16861 atacccaagt gaagttccat aacctgaccg atgttcatac gtgatggcac cccgagtggg
    16921 ttcaacatga tgtcaactgg tgttccgtct ggaagatatg gcatatcctc aacaggtaca
    16981 atacgtgaaa cgacaccctt gttaccgtga cgaccggcca tcttatctcc gaccttgatt
    17041 ttacgttttt gagcgatgta aacacgaacc aacatgttaa cacctgattg caattcatca
    17101 ccgttggcac gggtaaagat tttcacatca cgaacgacac catcggcacc gtgtggtaca
    17161 cgaagagagg tatcacgcac ttcacgtgac ttatcaccga agatagcgtg caagagacgc
    17221 tcttcagcag aaagatcttt ttcacctttt ggtgtaactt taccaacaag aatgtcgccc
    17281 tctttaactt cggcaccaat acgaatgata cccatttcat ccaagttgcg aagggcatct
    17341 tcaccaacgt ttggaatttc gcgtgtaatt tcttcagggc ctaacttggt atcgcgtgtt
    17401 tctgattcgt attcttccaa gtgaacagat gtatagacat catctttcac aaggcgttca
    17461 gacatgataa ccgcatcctc gaagttgtaa ccttcccaag tcatgtaggc aacgataggg
    17521 ttttgaccaa gagccatttc cccattttcc atagaaggtc catctgcgat gaagtcgcct
    17581 ttttctacga tgtcgcccaa ttttacaagg gtacgttggt tgtaggcagt acccgagttt
    17641 gaacgacgga atttagaaat gtggtagaca tcaagcgatc catcttcacg acgcacttct
    17701 accttgtccg catctgcgta aacaacctta ccatcgtgtt gagcgataat tgccgcacct
    17761 gagtcgtggg cagcctggta ttccatacca gtaccaacgt aaggtgcttt tggatcaatc
    17821 aatggaacag cctggcgttg catgttggca cccatgaggg cacggttgga gtcatcgttt
    17881 tccaagaaag gaatacatgc tgtcgcaacg gcaactacct gtttaggtga aacgtccatg
    17941 aagtctgctg aatctgctgg gaactcttgg ttgttacctt ggtgacgtcc cataacaatc
    18001 ttgtcaacga aacggttgtt ttcatcaagt ggcgatgttg attgtgctac gatgtaagca
    18061 tcttcttcat cagcagtcaa ccaaacaatt tcgtttgtaa ctgtaccagt tgcacggtca
    18121 atcttgcggt atggtgtttg gatgaaacca tacttgttca ggtgaccata agaagacaag
    18181 ttgttgatca aaccgatgtt aggtccttca ggcgtttcaa tcggacacat acgaccatag
    18241 tgagtgtagt gaacgtctcg aacctcgtaa ccggcacggt cacgagtcaa accaccaggt
    18301 cccaaggctg acaaacggcg tttgtgagac aactcagaaa gcgggttgtg ttgatccatg
    18361 aactgtgaca actgagatga accaaaaaat tctttgattg cggctgtaac aggacggata
    18421 ttgatgattt gttgtggcgt caatacttca ttgtcttgaa cagacatacg ctcacgcaag
    18481 ttacgttcca tacgcgtcaa accgatacgt acttggttag caagcaattc accaacggca
    18541 cgaatacgac ggttacccaa gtggtcaata tcatctacac gaccaagacc ctcagccaag
    18601 ttgaggaagt agctcatttc agccaagatg tccgcaggtg ttactgtacg tacgttttct
    18661 gctggattag cattaccaat caccgtcaca acacgttctg gatccttagg agctacaatc
    18721 ttgaatttct gcaaaagaac tggctcaaga agaacagcat tgtcatttgg aatatattct
    18781 accaagttga gttcatcgaa ttgtgcttct actttttcaa gcacatcacg actcaataca
    18841 gtaccagctt ccaagacaat ttcgcctgtc tcaccgttaa tcacatgctc agcaagtgtt
    18901 tggttgagca aacgagtacg gaggttgagt tttttattaa tcttgtaacg accaacaggt
    18961 gccaaatcgt aacgacgtgg gtcaaagaaa cgagctgtca acaggctacg agagctttca
    19021 gctgtctttg gttcacctgg acgaaggcgc tcatagattt ccttaagcgc ttcatccgta
    19081 cgagaatctg ctgggttttt atgaatatct ttttcaatgg tattgcgaac caattcgcta
    19141 tcaccgaaga tgtcaaagat ttcatcatca cctgagaaac caagcgcacg aacaagcgtt
    19201 gtgaacggaa tcttacgcgt acggtcaata cgagtgtagg caatgtcttt tgaatctgtt
    19261 tccaattcca accaagctcc acggttaggg ataaccgttg aaccataacc tactttaccg
    19321 ttcttatcta ctttatcatt gaagtagaca cctggtgaac gaaccaactg agaaacgatg
    19381 atacgctctg caccgttgat gatgaaggta cccatttcag tcatgattgg aaactcaccg
    19441 aagaagactt cttgtgtctt gatctcgcca gtttctttat tcaccagacg gaaagttaca
    19501 taaattggag ctgagtagtt cgcatcatgc gcacgcgcct cttccaatgt gtatttaggc
    19561 tctttcaatt cataaccaac gaattccaat tccatggtat ccgtaaagtt tgaaactgga
    19621 agtacatctt caaagacttc tttcaaaccg taatctaaaa agtcttggaa agagtccgtt
    19681 tgaatttcaa tcaaatttgg taaatcaaga acttccttga ttcttgaaaa actacgacgg
    19741 gtacggtgct taccgtactg aacttcatgt cctgccaaaa gttttctcct ttgtaataag
    19801 atacgagacc gtgaaaacca aagtgaaaat agaagagtaa cgccgtgtca ccagacacaa
    19861 ggagctctat ctttttcaca caggttttag gtcgagttca attaaagata cggggcgttt
    19921 aacactccaa aagaaaataa gcggtaagcc cgaaatcttt gatttcgttg acgcaccctc
    19981 gtcagaacga ctaggacttt ctagacagaa tgtcgaagaa agttcagacg tttacgacgc
    20041 caaggggtaa acaaagtcaa agttctacag gagtgtatcc cgagttcaat tatatacagc
    20101 catgtgatga ccaacattca ttctttttct gaattttcag aatctttaag aataggtaac
    20161 aattctcaaa aaaattcctc atagacacaa aaagagcagc taaatcttcc tttgaaaaag
    20221 tttgatttaa ctgctgcaag ccttgtttgg acaatatttc aaacaagaca cacgacaaat
    20281 aatttataca attatagcac aagtactagg aaaaatcaag gtgaatcatt atttgctcca
    20341 cgtcatacgc atgaaaaatt tcttctctcc ttttgtcccc agaaataact gctttaaact
    20401 tagtatctac ctttttacaa ccacctttat cctcgatgtc caaataattg tggcaaataa
    20461 tcctccaaat ggacaaaaat atagcgttgt ttgcggcgat agctcagctc ttccttaggg
    20521 aatgtcatat tttgtaaaag atgaagacaa actttacgaa tagcatttaa attgaaagca
    20581 gctcgtttat ctaaggtttg gttcctatct tctcggtaaa ccacatctaa caaccaatgt
    20641 aggctctcaa ccgaccaatg accacgaaca acctgagaaa aagtttgcac atcgcccttg
    20701 aaactaagga taaagtatcg aacttcttcc gtgatgatac cgtctttatc aatggtattc
    20761 ttagtcatac caatcccacg aagtttttcc cattttggat gtcgttgact caaccatctc
    20821 acatcgtgag aaatccagta gtccctcacc tcaatctgac tgcgtgcctt ttcaatggtc
    20881 tgataatgac agcctttcgc cgtcaactta gataacaatt tggcgtca
//
LOCUS       DATUCG010000024        20925 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.025, whole genome shotgun sequence.
ACCESSION   DATUCG010000024 DATUCG010000000
VERSION     DATUCG010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20925
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.025"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            185..1186
                     /locus_tag="VCA20_000731"
     CDS             185..1186
                     /locus_tag="VCA20_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEP1834584.1"
                     /translation="MLKNKNLLATIVALTVMVVAALFMTQKEQSNSSTSTEKVKIGVL
                     QFLTHDSLDEIYKGIKAGLKEGGYTTTDNLEIDFMNAEGDQSQVQTMSKKLVDNGNEL
                     LLGIATPAAQGLANATTELPIIMGAVTDPVGANLVTDLKNPGGNITGVSDQTPVADAV
                     SLIKEITPDAKTIGVLYSSNEDNSKIQVAEFKAAAEEAGYTVLEYAVASSNEIAATVE
                     VASSKADVLFTPVDNTVASAFSTVVSVANKTKTPIFTSVEDMVEGGGIASVTLSQYDL
                     GVATGKMAAKILDGANPADTPVQIFNEGTVVVNQKVAKELGITLSDDVISKASKVIE"
     gene            1209..2075
                     /locus_tag="VCA20_000732"
     CDS             1209..2075
                     /locus_tag="VCA20_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002902146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP1834585.1"
                     /translation="MIVSTVSQALLWAVLGLGIFVTFRILNFPDMTTEGSFPLGGAVA
                     VTLLTQGVHPILATLAAVLVGCLAGFVTGLLYTKGKIPTLLAGILVMSSCHSVILFIM
                     GRANLGLLNTKRLQDFLPFSEQINGLLLGIGSLAVVIGALMFFLYTRLGQAFIATGDN
                     PDMARSFGINTSRMELLGLVLSNGIIALAGALIAQQEGYADVSRGIGVIVVGMASLII
                     GEVIYGNLTMLERFLAIVVGAIFYQFLILVVISLGINTSYLRIFSAMILATCLMVPEL
                     KNKLLKGASLSR"
     gene            2072..2830
                     /locus_tag="VCA20_000733"
     CDS             2072..2830
                     /locus_tag="VCA20_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1834586.1"
                     /translation="MKAIVELKNATKVVTNGFDEERVILDNVNLTIYQGDFITILGGN
                     GAGKSTLFNVIAGTLPLTSGSVHILGEDVTNWPAEKRAKYLSRVFQDPKMGTAPRMTV
                     AENLLIAKYRGEGRGLVPRRLAQQTEEFAQLLPRVGNGLEKHLDTPAGLLSGGQRQAL
                     SLLMASLKQPELLLLDEHTAALDPKTSQSLMQLTDELIETKGMTALMVTHQMEDALRH
                     GNRLIVMKNGQIIQDQTAEEKSKMKLEDYYKLFD"
     gene            complement(2895..4130)
                     /locus_tag="VCA20_000734"
     CDS             complement(2895..4130)
                     /locus_tag="VCA20_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEP1834587.1"
                     /translation="MKKTNKQFISSSTFTVEEIEEILQAKIKEETQKVEDAITQEEKD
                     KEQAVIDTIQYDESKINIVSLFSGCGGLDLGVELAGLVAQYGQEKAYQLFQSKLSYDS
                     HRNKLINFIFSNDLFESANQSYLKNFPDTTYKSSKNIQKVSLFPKSNIMLGGFPCPGF
                     SVAGPRLLDDERNFLYIHYIRALMQSKPEFFIAENVKGLMTLGKGKVLEQISEDFAAA
                     GYVVTAHLVNARDYGVPQLRERVFIIGVRKDIQKKFNYVYQLPPATHGPEKEKYVTLK
                     DAIGDLPLEPSDVFDSTYSSIYMSRNRKKSWEEQSFTIQASARQAPQHPHGFPMKKID
                     KETWVFQGKHNRRLSVRECARIQTFPDWFEFSNGNKLNVTENHRLNEQYKQIGNAVPV
                     MLAEKIARPIVEFYYKNIL"
     gene            4243..5226
                     /locus_tag="VCA20_000735"
     CDS             4243..5226
                     /locus_tag="VCA20_000735"
                     /inference="COORDINATES: protein motif:HMM:NF021097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HaeIII family restriction endonuclease"
                     /protein_id="HEP1834588.1"
                     /translation="MAKQTVQGKGFEYACITTFYKALTELGMRCEIEESKAYLTAQDN
                     FQKLDGILQENMFQAAKASLNTILKAEPNLSNGQELVTLSILVDKSGGDGDVRDIVFI
                     KGETDWEIGISCKHNHHALKHSRLSNDIDFGLKWLGLPVSNDYFEAIKPVFTRLATLR
                     DETKHLDKAEQHKWTMFENKEVEIYLPVLEAFKFELLRLYEQHQEVPKLLIEYLLGRK
                     DFYKVIAKDKERKTIVQAFNLNDALSTNYQSIRPKTKVKGLSMVLPTRIFSLDYKRGS
                     KNTLELIMDNGWSISFRIHNASSRVEPSLKFDIQLIGIPQSVTNVEEYWEE"
     gene            5344..5829
                     /locus_tag="VCA20_000736"
     CDS             5344..5829
                     /locus_tag="VCA20_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HEP1834589.1"
                     /translation="MDKLVFESLADLGMDYTIVEHPPALTTEEADRYIEGLEGVRTKS
                     MFLTNKKKTAFYLLIMDDQKQLDMDQFRDLVGANRIRMASSDSLMEKMHLPAGAVSVF
                     GLLHNVDKDIQVFFDKEILSEPILTFHPNVNTKTIFVKTEDVLRFVEEIGFSATIVDL
                     G"
     gene            5835..6239
                     /locus_tag="VCA20_000737"
     CDS             5835..6239
                     /locus_tag="VCA20_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834590.1"
                     /translation="MRKCLAICSLLCCLFLVGCRTGAAYQAEIEPMEQSELYTEKEIE
                     AAILVVQDYFDHHFNSCKLLTIGYSGDNEKEFDEWAEHYGAEEAIIITSSFKVAAEGA
                     EPTLEPNSTHTDWKWILVWNVGGKWEHKGHGY"
     gene            6578..8239
                     /locus_tag="VCA20_000738"
     CDS             6578..8239
                     /locus_tag="VCA20_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEP1834591.1"
                     /translation="MSTIKIMALGGVRENGKNLYIAEVNEHIFVLDAGAKYPENEQLG
                     VDVVVPNFDYLEENKHRVAGVFLSHGHADAIGALPYLLEKVKVPVFGSHLTIELAKLL
                     VKGNNATKKFNDFHVINAETEIDFGDSVVSFFQTTHSIPESLGIVVKTDEGNIVYTGD
                     FKFDQAADKFYRTDFGRLAEIGNEGVLALLSDSANADSNVQVASMHEAGEEILNTIAD
                     CDGRVIVAAVASNIVRIQQIFDAAEATGRRVVLTGHDVENIVRTAIQLKKLRLVSERL
                     LVKPKDMNKYEDHELIILETGRMGEPLNGLRKMSIGRHRYVEIKEGDLVYIVTTPSIS
                     KEAVVARVENLIYKAGGTVKSITKNLRVSGHGNARDLQLMLNIMKPKYLFPVQGEYRQ
                     LDAHAKAALEIGMYPENIIIVKRGDVMAFEEGDFVHSGAVPAGDVMIDGNAMGDVGNI
                     VLRDRKILSEDGIFIVAITVNRREKKIISKAKVNTRGFVYVKKSKDILREASELVNTT
                     VENYFTKDSFDWAELKSAVRDDLAKFLFDQTKRRPAILPVIMEVK"
     gene            8304..9080
                     /locus_tag="VCA20_000739"
     CDS             8304..9080
                     /locus_tag="VCA20_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEP1834592.1"
                     /translation="MAIMKIEYHSETLDMSRQVTVLYPDRNRVENPDDNDIPVLYLLH
                     GMGGNQDSWLNRSTVERLVRYTNLIVVMVNTDKGWYTNTTYGMNYFDAIAVELPQILK
                     RFFPNMSDKREKNFIAGLSMGGYGTFKIAMMTNRFSHAASLSGALYFDFDNPAAAELG
                     SLSYWQGVFGDISDKQNPNNLLEIAKQSDKKTKFYAWCGEEDFLFEGHQKAVGELKEL
                     GFDIEASFGPGKHEWYYWNQQIEKVLAWLPIDFKLEERLS"
     gene            9146..9840
                     /locus_tag="VCA20_000740"
                     /pseudo
     CDS             9146..9840
                     /locus_tag="VCA20_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736142.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
     gene            9851..10216
                     /locus_tag="VCA20_000741"
     CDS             9851..10216
                     /locus_tag="VCA20_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834593.1"
                     /translation="MLRTITNTIKRYPEQALLFLYNAGIFAWMQSTSHSIMEQIGIDS
                     NWFDKIPEPIKAWTGASLESMQTLLNSSAWGWLIVSMILMLVIRFVKGLIKFVIMLII
                     IGGGLYLLWQNKELLSGLV"
     gene            10317..12215
                     /locus_tag="VCA20_000742"
     CDS             10317..12215
                     /locus_tag="VCA20_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEP1834594.1"
                     /translation="MIILSGNKIERSFAGEVLFNNINIQVGERDRIALVGKNGAGKST
                     LLKILVGEEAATSGDISTKRDLSLSYLAQDSRFESENTIYDEMLHVFDDLRMTEKRLR
                     SMEEQMGSLSGNELDQLMKTYDSLSEEFRLAGGFSYEADIRAILNGFKFDQTMWDMKI
                     SELSGGQNTRLALAKMLLESPELLVLDEPTNHLDIKTIAWLENYLVHYKGALIIVSHD
                     RYFLDKVATLTLDLTKHSLDRYVGNYSQFVELKEQKLQTELQNYEKQQKEIAKLEDFV
                     QKNIVRASTTKRAQARRKQLEKMERLDKPTTGQKSANMTFQSDKTSGNIVLTVENAAV
                     GYDGEILSQPISIDQRKLDAIAIVGPNGIGKTTLLKSIIGALPFIKGEAKLGANVEVG
                     YYDQTQSALPPSNTVLEELWSAFPTTPEVEIRNRLGAFLFSGEDVKKSVSMLSGGEKA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINRVAT
                     KVLEISETGSTLYLGDYDYYLEKKAELEVEVQPDQLESTSQSAGAMDYQAQKENQKEQ
                     RKLARRIEQIEAEIESIENRLSELNQAMLETNDIGQLTDYQKEIDQLTAQQENLMEEW
                     EELSEQLG"
     gene            12539..13015
                     /locus_tag="VCA20_000743"
     CDS             12539..13015
                     /locus_tag="VCA20_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HEP1834595.1"
                     /translation="MNFGQQIKDLRKKKGLTQEQFALKLNVTRQAVSNWENDKNLPDL
                     ELLILMSAVFSISLDQLILGGTDMNNMTEKLVKDGREGRRTQMHLTITIIGSFLMLLG
                     FVCFVIKANSVEYIDAEGILHENFYLIPIGFLFLLVGGMTSLISAITYLRFRKEHK"
     gene            13017..13850
                     /locus_tag="VCA20_000744"
     CDS             13017..13850
                     /locus_tag="VCA20_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEP1834596.1"
                     /translation="MTKHPRFLNSDQFFACPHCGQGLGLDQSSLRCPNRHIFDIAKQG
                     YVNLAPQVKQSANYHKSSFENRKAFLEAGYYDHLYAALEGKIAELGLRSVLDIGCGEG
                     FYSRKLAEKMDLDILAFDISKDSILLAAKTDTTKSVKWFVGDLTKLPIQDKTIDGILD
                     IFSPANYQEFARVLKAGGAILKLVPGPNHLKELRYLAKDQLRKESYDNQDIVDHFKSY
                     VGQVEQVMISRTLPIIAAHAQVLADMTPLFFQVDQSKLDLGQLTEITVEAVLLVGTIV
                     K"
     gene            complement(14008..14793)
                     /locus_tag="VCA20_000745"
     CDS             complement(14008..14793)
                     /locus_tag="VCA20_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEP1834597.1"
                     /translation="MVYTSLKSPEKDYTYDLHIAHLYGDLMNTYGDNGNILMLKYVAE
                     KLGARVQVDIVSLTDDFDKDYYDIAFFGGGQDYEQSILAKDLPIKKSSLADFIENDGV
                     MLAICGGFQLLGQYYIEAGGRKIDGLGIMSHYTLNQTNNRYIGDIKIHNDEFNETYYG
                     FENHQGRTFLADNQKPLGKVVYGNGNNKEDGGEGMHYKNTFGSYFHGPILSRNANLAY
                     RLVTTALRKKYGQEIQLVSYTDILSKEVAEEYGDVKSKAEFEK"
     gene            complement(14793..16136)
                     /locus_tag="VCA20_000746"
     CDS             complement(14793..16136)
                     /locus_tag="VCA20_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEP1834598.1"
                     /translation="MKINTALGILAGKSSQFILKKLGRGTTLPGKIALKFDKHILDGL
                     AKDYEVVVVTGTNGKTLTTALTVGILQEAFGEITTNTSGANMITGITATFLSAKKNKK
                     GKKIAVLEIDEASLARVTDFIKPSLIVFTNIFRDQMDRYGEIYTTYQMILDGAAKAPQ
                     ATILANGDSPLFNSTKVVNTVKYYGFATEEHEPRLAHYNTEGVLCPHCHQIIQYKLNT
                     YANLGSYICNNCGFSRPELDYKLTDLKEITNTSSTFAIDGQDYKINIGGLYNIYNALA
                     AVSVAAFFGVSPEKIKAGFDKSKAVFGRQETFKLGDKDCTLVLIKNPVGATQAIEMMK
                     LAPYDFSLSVLLNANYADGIDTSWIWDADFEQITQMDIPQVFAGGVRSSEIARRLRVT
                     GYPEDQITETPKLEDIMTLIEQSSSQQAYILATYTAMLEFRDLLAQRQAVGKEMK"
     gene            16317..17171
                     /locus_tag="VCA20_000747"
     CDS             16317..17171
                     /locus_tag="VCA20_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEP1834599.1"
                     /translation="MLNLNQLLDTGYWSSLIASPWTALLHLIDISIVVYLIYNFSKAI
                     AGTKIMTLIRGVFLFIIVQIIASLFGLQTIAWLINQVITYGVIAAVVIFAPELRAMLE
                     KLGRTTQIFGTNTVSAEEKLIVAFLKSVAYMSPRKIGALVAVEQAQTLQEYRATGIPL
                     NADISQELLINIFIPNTPLHDGAVIVKEDKVAVACAYLPLSESAGISKEFGTRHRAAI
                     GLSEVSDAFVFIVSEETGSISIARNGIFKHDLTLDEFEAELRATFISENVEKKSIWKR
                     LGGGKHER"
     gene            17161..17865
                     /locus_tag="VCA20_000748"
     CDS             17161..17865
                     /locus_tag="VCA20_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834600.1"
                     /translation="MNDKFKAVGHLALSIFLALLLFFYATTTNYKNSESAAQNTESET
                     YVHTLSNVPIEIEYDTNKYFISGLSSTVAVELRGSNRVLLQRESDESTRTFQVTADLR
                     ELSDGAQTVKLQLTNLPAGVSATLAPDAITVKIGKKVSKAFPVAGRVYSNQLAEGYSL
                     SKVSVNVDTVKVTTDEETMAKIDRVEAVAMDVSNLSENYSGTAKLQAVDSDGNVLPVV
                     LSQTEANMQIILTKTK"
     gene            17878..19230
                     /locus_tag="VCA20_000749"
     CDS             17878..19230
                     /locus_tag="VCA20_000749"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEP1834601.1"
                     /translation="MGKYFGTDGVRGEANVELTPELAFKLGRFGGYVLSQHETDVPRV
                     LVARDTRISGQMLEAALIAGLLSVGIHVYKLGVLATPGVAHLVKTEKASAGVMISASH
                     NPAQDNGIKFFAGDGFKLDDALEAEIEALLDAEEDTLPRPSAKGLGDVVEYPEGLRKY
                     QQFLVSTGTDLDGMKVALDTANGAAATSARQIFVDLGADLTVMAEKPDGLNINEGVGS
                     THPEKLQELVKETGSQIGLAFDGDSDRLIAVDENGDLVDGDRIMYIVGKYLADRGLLA
                     KNTIVTTVMSNLGFHKALDREGIEKAVTAVGDRYVVEEMRKEGYNVGGEQSGHVILMD
                     YNTTGDGQLTAVQLTKIMKETGKKLSELAAEVTIYPQKLVNIRVENSMKDKAMEVPAI
                     AAIIEKMEAEMAGNGRILVRPSGTEPLLRVMAEAPTDAEVDYYVDTIADVVRAEIGLD
                     "
     gene            19279..20852
                     /locus_tag="VCA20_000750"
                     /pseudo
     CDS             19279..20852
                     /locus_tag="VCA20_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025412.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
BASE COUNT         5994 a         3938 c         4963 g         6030 t
ORIGIN      
        1 tattgcgtca cgtttggctt gcctaacttg agttatgtct gcgcctccgt tcctattcta
       61 aaagcaatct actttgacaa ttattaacaa aacgaggtaa tcatgatgaa acgatggcga
      121 tggtggaata aaattttgaa gacgtagaaa ttggatattg tgcattgtta aaaggagaat
      181 gaatatgttg aaaaataaaa atttacttgc tacaattgtt gcccttactg ttatggtagt
      241 tgctgctttg tttatgacac agaaagagca gagcaattca tctacatcaa cggagaaagt
      301 taagattggg gttcttcagt ttctaaccca tgattcacta gatgagattt ataagggaat
      361 caaggcaggc cttaaagaag gtggttatac cacaacggat aatttagaaa ttgattttat
      421 gaatgcagaa ggtgatcagt cacaggttca aaccatgagt aaaaaattgg tggacaatgg
      481 caatgaatta ttgcttggta tcgcaacacc tgctgcacag ggattagcaa acgctacgac
      541 agaattaccc attatcatgg gagctgtgac agatcctgtt ggtgctaact tggtaactga
      601 cttgaaaaat cctggtggaa atattacagg ggtatcagac caaacaccag tagcagacgc
      661 agtttctctc attaaagaaa ttacaccaga cgctaaaact atcggtgtcc tctattcttc
      721 aaatgaggac aattcaaaaa ttcaagtagc ggaatttaag gcagcagcag aagaggctgg
      781 ctatactgtt cttgaatatg cagttgcttc tagtaatgaa attgcagcga cagtagaggt
      841 cgcaagtagt aaagcagatg ttctctttac accagtcgac aacacagttg cctcagcatt
      901 ttcaactgtc gtatctgtgg caaataaaac caaaactccg atttttacca gcgtagaaga
      961 tatggtagaa ggcggaggta tcgcatctgt tacccttagc caatatgact tgggtgtggc
     1021 aactggtaaa atggctgcga aaattcttga tggtgccaat ccagcggata cacctgtaca
     1081 aatctttaac gagggtaccg ttgttgtcaa ccaaaaagtt gctaaagaat taggaattac
     1141 cttgtcagat gatgtgatta gtaaagcaag caaggttatc gaataagaga taagagaaaa
     1201 ggaaaaatat gattgtatca acagtttcac aggctctttt atgggctgta ctaggtttgg
     1261 gaatattcgt tacctttcgg attctgaatt ttcctgatat gacgacagag ggctctttcc
     1321 cacttggagg agcggttgct gttaccttat tgacgcaagg agttcatcct atcttggcta
     1381 ctctagcagc agttcttgtt ggctgtttgg cgggctttgt cacaggcttg ctttatacca
     1441 aggggaaaat tcccactctc ctagctggta tcttggtcat gtcttcctgt cactctgtca
     1501 ttctatttat catgggtagg gcaaatctag gcttactgaa taccaaacgt ttacaagatt
     1561 tccttccttt ttctgagcaa attaatggtc tcttgctagg tataggtagc ctagcggtgg
     1621 tgattggtgc cttgatgttc ttcctctata ctcgccttgg acaggccttt attgcaaccg
     1681 gggataatcc agatatggct cgcagttttg gtatcaatac aagtcgaatg gagttgctag
     1741 gccttgttct ttcaaatggg atcatcgctc ttgcaggtgc cttgattgca caacaagaag
     1801 gctatgcgga tgtgtcgcgt ggtattggtg tcattgtggt cggtatggct agtctgatta
     1861 tcggtgaggt aatttatggt aaccttacta tgttagaacg attcttggcc attgttgtcg
     1921 gagcgatttt ctatcagttc ttaattttgg tggtcatttc cctcggtatc aataccagct
     1981 acctccgtat cttctcggcc atgattctgg caacttgcct catggttcct gagttgaaaa
     2041 ataaacttct gaaaggagcc agtctgtcac gatgaaagca attgttgaat taaaaaatgc
     2101 caccaaggtg gtgacaaacg gttttgatga agaacgtgtg attttggata atgtcaatct
     2161 gaccatctac cagggtgatt ttattaccat tttgggtgga aatggggctg ggaagtcaac
     2221 cctctttaat gtcattgctg gaaccctacc cttgactagt ggttcagtcc atattcttgg
     2281 tgaggacgtg accaattggc ctgcagaaaa acgtgctaag tatctgtcac gtgttttcca
     2341 agatcctaaa atgggtacag cgccacggat gacggtggca gaaaatctac tgatagctaa
     2401 gtaccgtggc gagggacggg gcttagtgcc acgcagattg gcccaacaga cggaagaatt
     2461 tgcccagctt ttgccgagag tgggaaatgg tctagaaaaa catctggaca cgccagcagg
     2521 gctcttatca ggtggtcaac gccaggcatt gagtttgttg atggcgagtt tgaaacagcc
     2581 tgaactttta ctcttggatg agcatactgc agcccttgac cctaagacca gccaatcctt
     2641 gatgcaatta acggacgagt tgattgagac caagggaatg actgcgctta tggtaactca
     2701 tcagatggaa gatgctttgc gtcacggtaa tcgcttgatt gtcatgaaaa atggtcagat
     2761 tattcaggac caaacggctg aagaaaaatc caagatgaaa ctggaagatt attataagtt
     2821 gtttgattag tttgatgttt atgtgtcaat aaagataaag aaagaatcct gtgtttgggc
     2881 aggattcttg ttttttatag tatatttttg tagtaaaatt cgactatagg tctggcaatt
     2941 ttttcagcca gcataacagg tacagcattt cctatttgct tatattgttc atttaatcga
     3001 tgattctctg ttacatttag tttatttccg ttagaaaatt caaaccaatc gggaaatgtt
     3061 tgaatgcgag cacattctct gacagatagt ctacgattat gttttccttg aaagacccat
     3121 gtttccttat ctattttctt catagggaat ccgtggggat gttgaggtgc ttgtctagca
     3181 ctggcttgaa tcgtaaagct ctgttcttcc caagatttct tacggtttcg gctcatatag
     3241 atagaagaat atgttgaatc aaagacatca cttggttcaa gaggtaaatc tccaattgca
     3301 tctttgagtg tcacatattt ttctttttct ggaccgtgtg ttgcaggtgg cagttgatag
     3361 acatagttga atttcttctg aatatctttt cttacaccaa taataaaaac acgttctctt
     3421 aattgtggaa ctccataatc tcgtgcattt acaaggtgag cagtaacgac atagccagcc
     3481 gcagcaaagt cttctgaaat ttgttctagc acttttcctt ttccgagagt cattagacct
     3541 ttaacatttt ctgcaataaa gaactcaggt tttgactgca tgagtgcacg gatgtaatga
     3601 atgtataaga agttacgctc atcatcaagc aagcgtggac ctgcaacact aaagcctgga
     3661 caaggaaatc ctccaagcat gatattggat tttggaaaaa gggaaacttt ttggatattt
     3721 ttagaacttt tgtaagtcgt atcagggaag ttttttaggt aagactgatt tgctgattca
     3781 aataaatcat ttgaaaaaat gaaattgata agcttgtttc tgtgactatc ataggacagc
     3841 tttgattgga atagttgata agctttttct tgaccgtact gagcaacaag gccagctaat
     3901 tcgacaccta aatcaagtcc accacagcca ctaaagaggg aaacaatatt gattttgctt
     3961 tcatcgtatt gaatagtatc aataacagct tgttccttat ctttttcctc ttgagtaatg
     4021 gcatcttcga ctttttgagt ttcttctttg attttagcct gtaatatttc ttcaatctct
     4081 tctacagtga aggtggatga agatataaat tgcttgtttg tttttttcat aagaactccc
     4141 aactgaatat atactttaca agtataacaa aaaaaaataa acttggccag tagaaactat
     4201 tgtgggataa taaaggtagt aaaaatatat aggagtagta gaatggctaa gcagacagta
     4261 caaggaaaag gatttgaata tgcttgtatt acaacttttt acaaggcttt gacagaattg
     4321 ggtatgagat gtgaaattga agagagcaag gcctatctaa ctgcacaaga taatttccaa
     4381 aaattagatg gcattttaca ggaaaatatg tttcaagcag ctaaggcgag tctaaatact
     4441 attttaaagg cagaaccaaa tctttctaat ggacaggaat tagtgacctt atccatttta
     4501 gttgataaaa gtggtggaga tggtgatgtt agggatattg tatttatcaa aggggaaact
     4561 gattgggaga ttggaatttc ttgtaaacat aatcatcacg ccctaaaaca ttctaggcta
     4621 tccaatgaca tagattttgg tttgaaatgg ttggggcttc cagtttctaa cgattacttt
     4681 gaagccatca agcctgtatt cactaggtta gcaactttga gggatgaaac caagcacctt
     4741 gataaagccg agcaacataa gtggactatg tttgaaaata aagaggtgga gatttattta
     4801 cctgttcttg aagcttttaa atttgagttg cttcgtctat atgagcaaca tcaggaagtc
     4861 cccaaacttc taattgagta tctactaggg cgaaaagatt tttataaagt catcgcaaag
     4921 gataaggagc gaaaaacgat tgttcaagca tttaatctaa atgatgctct atctacaaac
     4981 tatcaatcca tacgaccaaa gacaaaagtc aagggattat cgatggtttt accaacaaga
     5041 attttctctt tagattataa aagaggttct aaaaatactc tggagttgat tatggataat
     5101 ggttggtcca tcagtttccg tattcataat gcctcttcga gagtggaacc ttccttaaaa
     5161 tttgatattc agttaattgg tatccctcag agtgtgacaa atgttgaaga gtattgggaa
     5221 gagtgattat cgacatacgg caatctgttt gacgaaagtc tggagtaatc caggctttta
     5281 ttatcctttg aatttttagt taaaaagcag tataatatac taaatatgtc aagaggtgct
     5341 gtcatggata aattggtttt tgaaagctta gctgacttgg gaatggacta caccattgta
     5401 gagcatccac cagctctgac aactgaggaa gcagaccgtt acattgaagg gttagaagga
     5461 gtacggacca agtccatgtt tctgactaat aagaaaaaga ctgcatttta tttactgatc
     5521 atggatgacc agaagcagct ggatatggac cagtttcgtg atttggtggg agccaaccgc
     5581 attcggatgg cctcgagtga tagcttgatg gaaaaaatgc acttgcctgc gggtgcggta
     5641 tctgtttttg gcttgttaca taatgttgat aaggatattc aagtcttttt tgataaagaa
     5701 atcctttctg aacccattct aaccttccac cccaatgtta atacgaaaac aatttttgta
     5761 aagacagagg atgttttgcg ttttgtagaa gagattggct tttcagcaac gattgttgac
     5821 ttggggtaac aaagatgaga aaatgtttag ctatttgtag tctgttgtgt tgtctttttt
     5881 tggtaggatg tcggacagga gcagcttatc aagcagagat tgaaccgatg gaacaatcag
     5941 aattgtatac ggaaaaagag attgaggcgg ctattcttgt agttcaagat tactttgatc
     6001 accattttaa cagttgtaaa ttgctgacta tcggttatag tggtgataat gaaaaggaat
     6061 ttgatgagtg ggcggagcac tatggtgcag aggaagctat tatcataact tcgtctttta
     6121 aggtagcagc tgagggggca gagcccacct tagagcctaa tagtactcat acggattgga
     6181 aatggatttt agtgtggaat gttggtggta agtgggaaca taagggacat ggttactaat
     6241 gtatatgtag aacttcgttg attttcagcg gagttttttg taaattttct tgaaaaaatg
     6301 tataatagaa gcgatagcta aaatatagtc ttttagttta cttttagtac taggcaacga
     6361 gccgcaggca taattggagt caggcaaggc gagttcaaat actcctgtgg agtgtttgaa
     6421 gttggaaata gggaaacgaa gtttcctcgt acgtcgaagt aataaaagaa aaactaaatg
     6481 acgataaata gcagactgta attcgacttg ggcatcccaa ggaccgttgg taagaaaaat
     6541 aaaatggaca cctgtgggat gtccaaaagg agtaaagatg agtacaatta aaattatggc
     6601 tcttggaggt gtgcgggaaa atggaaaaaa cctgtacatt gccgaagtga atgaacatat
     6661 ttttgttttg gatgcggggg caaagtatcc tgagaatgaa cagttaggtg tggatgtagt
     6721 cgtccctaat ttcgattatt tggaagaaaa taagcatcgt gtagcagggg tctttttatc
     6781 tcacgggcat gcagatgcta tcggagcgct tccttatctc ttggagaaag tgaaagtgcc
     6841 agtatttggt tcgcacttga ctatcgaatt ggctaagcta cttgtcaagg ggaataatgc
     6901 tactaagaaa ttcaatgatt tccatgttat caatgctgaa actgaaattg atttcgggga
     6961 ttcagtggta tctttctttc agacgaccca ctctattcca gagagtttag gaattgtcgt
     7021 taagacggat gaaggaaata ttgtctacac aggtgacttt aagtttgacc aggcggcaga
     7081 taagttttat cgtacggatt ttgggcgatt ggctgaaatc ggtaacgaag gtgtgttggc
     7141 cctcttgtca gactcagcaa atgcagatag caatgttcaa gtagcgagca tgcatgaggc
     7201 tggggaggaa attctcaata cgattgctga ctgtgacggg cgtgttattg tggcagcagt
     7261 tgccagcaat attgtacgta ttcaacagat ctttgatgcg gcagaagcaa ctggtcgtcg
     7321 tgtcgtgttg acaggacatg atgtggaaaa cattgtccga acagcgattc aattgaaaaa
     7381 acttcgtttg gtcagcgaac gcttgttggt aaaaccaaag gatatgaaca agtatgaaga
     7441 ccatgaattg attattttag aaactggtcg tatgggcgaa ccgttaaatg gattgcgtaa
     7501 aatgtctatc ggtagacatc gctatgttga aatcaaagaa ggtgatctag tttatattgt
     7561 taccactcca agtatttcaa aagaagcggt tgttgctcgt gtggagaatt tgatttacaa
     7621 ggctggtggt accgtaaaat ctatcacaaa gaatcttcgc gtctctgggc atgggaatgc
     7681 gcgtgatttg cagctcatgc tcaacatcat gaagcctaaa tatcttttcc cagttcaagg
     7741 ggagtatcgt caattggatg ctcacgctaa ggctgcgctt gaaatcggta tgtatccaga
     7801 aaacattatc atcgttaagc gtggggatgt catggctttt gaagagggag attttgtcca
     7861 tagcggagcg gttccggctg gtgatgtcat gattgacgga aatgccatgg gcgatgttgg
     7921 aaatatcgtc cttcgtgacc gtaagatcct ctcagaagac ggtatcttta tcgtggctat
     7981 cactgtcaac cgccgtgaga aaaaaatcat ttctaaagcc aaggtcaata ctcgtggttt
     8041 tgtctacgtg aaaaagagca aggacatctt gcgtgaggca tcagaattgg tgaatacaac
     8101 ggtggaaaat tactttacaa aggatagctt tgactgggca gagcttaaga gtgctgttcg
     8161 cgatgattta gcaaaattcc tctttgacca aaccaaacgt cgcccagcta ttttgccggt
     8221 gattatggaa gtcaagtaag ctgatttata tatataagaa gagccgaggg aaactcaggc
     8281 tctttaccat aaggagaaga tttatggcta taatgaaaat tgaataccac tcagaaactc
     8341 tagatatgtc tagacaggtt acggttttat atccagatcg caatcgtgtg gaaaatcccg
     8401 atgacaacga cattcctgtc ctatatcttt tgcacggtat gggaggaaat caggattcct
     8461 ggctgaatcg ttctacagtg gaacggttgg ttcgttatac caatcttatc gtggttatgg
     8521 tcaatacaga taaaggttgg tataccaata ccacttacgg tatgaattac ttcgatgcca
     8581 ttgcagtaga gttgcctcaa attctcaaac gattttttcc aaatatgtct gacaagcggg
     8641 agaagaattt tatcgctggt ttgtctatgg gtggttatgg gaccttcaag attgccatga
     8701 tgaccaatcg tttttcacac gcagcttcgc tttcaggcgc attgtatttt gattttgata
     8761 atccagcggc agcagaatta ggaagccttt cgtattggca aggagtattt ggtgacattt
     8821 ctgataaaca gaatccaaat aatttgttag aaattgctaa acaatccgat aaaaagacca
     8881 aattttatgc ctggtgtggt gaggaagatt ttctctttga agggcatcaa aaggcagttg
     8941 gggagttgaa agagctggga tttgatatag aggcaagttt cggacctggt aaacatgagt
     9001 ggtactactg gaatcagcag attgagaagg tcttggcttg gttgccaatt gatttcaaat
     9061 tggaagaaag actgtcctag tttttaagaa aagtttcaga aattatggct atactaaaac
     9121 ctatttctag aaagaggtat agtctatgaa acttattgga aatattctac ggtggatttg
     9181 gcggacattt tggggtctga tttggttatc aatggtgtta ttggcttgtg gtctaggtct
     9241 gttattctat tttcaacaga aagcagcgcc tcagatgcta caaactttag ctcaagaagt
     9301 gcaattgatg gtcaaaggac agtctgaagt gacgattgaa gaaggtgtta ataccattaa
     9361 gcacctgacg actgatacgg ttaatacagc tgatcatggt cgatgggaaa gcaatactgc
     9421 gactgtttac attgaaaccc agaatccaac atttgtagct gcctatgaaa cagccattgc
     9481 aaattggaat gcgacagggc cttcactttt gtgatgacaa gtgatccaaa tcaggctgat
     9541 attattgcta ctgagatgaa tgatggaaat acccaggcag caggcgaggc caattcaacg
     9601 actaatttat tgaccaatta ttatagctcg gtaacggtgc gtctgaatag tttttattta
     9661 ttaaacgacc aatatggtta tgatatggat agaattatcc atacagctga gcatgaattg
     9721 ggccatgcta ttggcctaga tcatgaagat agtcagacct ctgttatgga atcggcaggt
     9781 tctaatcatg gtatccagca ggcagatatt gatgcggtcc ttgccttgta ttcagaatag
     9841 aagggaaaat atgcttcgaa caattacaaa cacaataaaa cgttatcctg agcaagctct
     9901 gctctttctc tataatgctg ggatttttgc atggatgcag tctaccagtc attcgattat
     9961 ggaacaaatc ggtatagata gcaactggtt tgataaaatc cccgaaccta tcaaggcctg
    10021 gacaggtgca agtctagaga gtatgcaaac cttactcaat tcttccgctt ggggatggct
    10081 gattgtatct atgatattga tgttggtcat ccgctttgtt aagggcttga tcaagtttgt
    10141 catcatgctg attattatcg gcggtggtct ttatctgctc tggcagaata aggaattgct
    10201 tagcggtttg gtctaaatat tttttgagaa ggacagtggc tgaaaagtcg ctgttttttg
    10261 ctgaaatcag aaaaaggatt ccgcactgtt accctaaatt atggtacaat attgctatga
    10321 ttattctaag tggaaacaag attgaacgct cttttgcggg cgaagtttta tttaacaata
    10381 tcaatattca agtcggtgaa cgggatcgga ttgcccttgt cggaaaaaat ggggcaggca
    10441 agtctactct gctcaaaatc cttgtcggag aagaagcagc gaccagcggt gacatttcaa
    10501 ccaagcgtga tttgtccctt tcttacctgg cacaggacag ccgttttgag tcggaaaata
    10561 ctatctacga tgaaatgctc catgtctttg atgatctgcg gatgacagag aaacgcctgc
    10621 ggtctatgga agagcaaatg ggcagtcttt ctggtaatga actcgatcag ctcatgaaga
    10681 cctatgatag tctgtcggag gaatttcgtt tggcaggtgg ttttagctat gaggcagaca
    10741 tccgtgctat tttgaatggt ttcaagtttg accagaccat gtgggacatg aagatctccg
    10801 aactctcagg tggtcagaat actcgacttg ctctggcaaa aatgcttctt gaaagcccag
    10861 agttattggt cctggacgag ccgaccaacc acctggacat caagacgatt gcttggttag
    10921 aaaactacct agtgcattac aagggagcct tgattatcgt cagccatgac cgctatttct
    10981 tggacaaggt ggcgacactg acgcttgatt tgaccaagca ttccttggat cgctatgtgg
    11041 gtaattattc tcagtttgtc gagctcaaag agcagaagtt gcaaacggaa ttgcaaaact
    11101 atgaaaagca gcagaaggaa attgccaaac tagaagattt tgtccagaaa aatatcgtcc
    11161 gtgcttcgac caccaagcgt gctcaggcta gacgcaagca gttggaaaag atggagcgac
    11221 tggacaagcc gacaactggt cagaagtctg ccaacatgac ctttcagtcg gacaagactt
    11281 ctggcaatat cgtcttgaca gtggaaaatg ctgcggtggg ctacgatgga gaaatacttt
    11341 ctcagcctat ttccattgac caacggaaac tcgatgccat tgccattgtc ggtcccaatg
    11401 gaattggaaa aacaaccttg ctcaaatcaa tcatcggagc tctgccattt atcaaagggg
    11461 aagccaagct cggtgccaat gtggaagtag gctactacga ccagacccag tcggccctgc
    11521 cgccttccaa cacggtcttg gaggaactct ggtcagcctt tccgaccaca cctgaagtgg
    11581 aaattcgtaa ccgcctagga gcctttctct tctcaggaga ggatgtcaaa aagtccgtct
    11641 ctatgctatc tggtggggaa aaagcccgtc tgctcctcgc caagctatcc atggaaaaca
    11701 ataactttct catcctcgac gagccgacca accacctgga cattgacagc aaggaagtcc
    11761 tggaaaatgc cctcatcgac tttgacggca ccctgctctt tgtcagccac gaccgctatt
    11821 tcatcaaccg cgtcgccacc aaggtcctgg aaatctcgga aacaggctcc acgctctact
    11881 tgggcgacta tgattattat ttggagaaaa aggcagagct ggaagtggaa gtccagcctg
    11941 atcagctaga aagtactagc caatcagccg gagccatgga ctaccaagcc caaaaggaaa
    12001 accaaaaaga gcaacgcaaa ctagcccgcc gcatcgagca aatcgaggca gaaattgaga
    12061 gcatcgaaaa ccgcttaagt gagctcaacc aagccatgct agaaaccaac gacatcggcc
    12121 agctgaccga ttatcagaaa gaaatcgatc aattgactgc ccagcaagaa aaccttatgg
    12181 aagaatggga agaattatct gagcaattag gctaggttga cagcaagttg acaagaggct
    12241 caaatccttg atagaacaag cttgcaagcc agtcttgcgg agttagcacg accacctttc
    12301 gctaaaaaag tccagagcaa gcccgccttt cttggaaaac atcaaccgac aggctaagat
    12361 aagtatagag attaacatca cttctcgaag gtggtgtcaa catctcagcg cagtggttga
    12421 ttggcagatt tgttcgtgtt gcacacttca aatctggcct aatcaactgt gcgggggtgg
    12481 gacgacgaac tctttttcat ttttcgagtt ctgtcccgct ccccgaaagg agaacgaaat
    12541 gaactttgga cagcaaatca aagacttacg aaaaaagaaa ggtttgactc aggaacagtt
    12601 tgccctcaaa ctcaatgtga ccaggcaggc tgtttccaac tgggaaaatg acaagaatct
    12661 acctgatttg gagctcttga ttctcatgtc cgctgtcttt tcgatctcct tagatcaact
    12721 tatcttagga ggaactgaca tgaacaacat gacagaaaaa cttgtaaaag acggtcgtga
    12781 aggtcgccgt acccagatgc acctgaccat taccattatt ggtagttttc tcatgcttct
    12841 cggcttcgtc tgctttgtca tcaaggcaaa ctcggtcgag tatatcgatg ccgaaggcat
    12901 tctacatgag aacttttacc tgattccaat tggcttcctc ttcctattag taggaggcat
    12961 gaccagtttg atttcagcaa tcacctatct tcgctttaga aaagaacaca aataagatga
    13021 ccaaacaccc acgtttcctt aatagcgacc aatttttcgc ctgtccacat tgtgggcagg
    13081 gtcttggtct tgaccagagc agtcttcgct gccccaaccg ccacatcttc gacatcgcca
    13141 agcagggcta cgtcaatctg gcaccccagg tcaagcagtc tgctaactac cacaagtcta
    13201 gctttgaaaa ccgcaaggcc tttttagagg cgggctatta cgatcatctt tatgcggctt
    13261 tggagggaaa aatagcagag ctggggttac gttctgtcct ggacatcggt tgtggggagg
    13321 gcttttattc ccgtaagtta gccgagaaaa tggacttgga cattcttgct tttgatattt
    13381 ccaaggattc catactcctg gcagccaaaa cagacactac caagtcggtc aaatggtttg
    13441 tcggtgactt gaccaagttg cccattcaag ataagaccat cgacggcatc ttggacatct
    13501 tctctccagc caattatcag gagtttgcca gagtgttgaa agctggtggg gcaattctaa
    13561 aattagtccc aggtccaaat cacctcaagg aactccgcta tttagccaaa gaccaactcc
    13621 gcaaggagtc ctatgataac caagatatcg tagaccattt caagtcatac gtggggcagg
    13681 tggagcaagt gatgatcagc cggaccctgc cgattatagc cgcccatgct caagtcttgg
    13741 cagacatgac gcccctcttt ttccaagtgg accagtccaa gttggatttg gggcaattaa
    13801 cggagattac ggttgaggca gtacttttgg taggaactat agtcaagtga attagaaaca
    13861 ggacatatct aagtttgtca atttgagaac tctaaagtta ttttaggagt gggttattaa
    13921 cactataact taagtctgcc cttttcctaa ttcaagtcac tatatatgaa aaatgaaacc
    13981 gcctctgatt tcagaagcgg ttttgcttta tttctcaaat tctgccttgc tttttacatc
    14041 gccgtattct tcagcgactt ctttgctgag gatgtcagta tagctgacaa gttggatctc
    14101 ttgtccgtat tttttacgga gggcagttgt gaccaagcgg taggctaggt tggcattgcg
    14161 agacaggatt ggtccgtgga agtagctgcc gaaggtgttt ttatagtgca taccttcgcc
    14221 accgtcttct ttattgttgc cattaccata gacaaccttg cccagcggtt tttggttgtc
    14281 agctaggaag gtacggcctt ggtggttttc aaaaccgtag taggtttcgt tgaattcatc
    14341 attgtgaatt ttgatgtcac cgatgtagcg gttgttagtc tggttgaggg tgtagtggct
    14401 cattataccc agtccgtcaa tcttgcggcc gccagcttcg atgtagtatt gaccgagcaa
    14461 ttggaagcca ccgcagatgg ccagcatgac accatcgttt tcgataaagt ctgctaggct
    14521 gcttttcttt atcggtaaat ccttggctaa aatagactgc tcgtagtctt gaccaccacc
    14581 gaaaaaggcg atgtcatagt aatccttatc aaaatcatca gtcagtgaaa cgatgtccac
    14641 ctgaacacga gcaccgagct tttcagccac atacttgagc atgaggatgt tgccgttgtc
    14701 cccgtaggtg ttcatcaaat caccgtaaag atgggcgatg tggagatcgt aagtgtagtc
    14761 tttttcagga ctttttaatg acgtatagac cattatttca tctcctttcc aacggcttgc
    14821 cgttgagcca gcaggtcgcg gaattccagc atggctgtat aggtcgcgag gatataggcc
    14881 tgttgactgc tggactgctc gatcagggtc atgatgtctt cgagttttgg tgtttccgta
    14941 atctggtcct ctggatagcc agtcacacgg aggcgacggg cgatttcaga cgagcgaaca
    15001 ccgcctgcaa agacttgtgg aatgtccatt tgagtgattt gttcaaagtc agcatcccaa
    15061 atccagctgg tatcaattcc gtcagcgtag ttggcattga gaaggacgga caggctgaaa
    15121 tcgtatggag ccagtttcat catttcaata gcctgggttg caccgacagg gtttttaatc
    15181 aagaccaatg tacaatcttt atcacccaac ttaaatgttt cttggcgacc gaagactgcc
    15241 tttgatttgt caaatcctgc cttgattttc tcaggagata cgccgaaaaa tgctgctacg
    15301 ctgacagctg ctagggcatt gtagatattg taaagtccac cgatgttgat tttgtaatcc
    15361 tgaccgtcaa tggcaaaggt agaagaagta ttggtgattt ctttcaagtc tgtcaactta
    15421 tagtccagct ctggtcggct aaagccacag ttgttacaga tgtagctgcc caagttagcg
    15481 taggtattga gcttgtactg gataatctgg tggcaatgag gacagagaac gccttcggta
    15541 ttgtaatggg ccagacgtgg ctcatgttct tccgtcgcaa aaccgtagta tttaacagtg
    15601 ttgacgactt tcgttgagtt gaaaagcgga ctatctccat tggccaaaat agttgcctgc
    15661 ggagcttttg cagccccgtc caaaatcatc tggtaggtgg tataaatctc accgtagcga
    15721 tccatttggt cgcggaagat gttggtaaag acaatcaagc taggcttgat aaagtcggtc
    15781 acgcgggcaa ggctggcctc atcaatctcc aaaacggcaa tcttcttgcc ctttttgttt
    15841 ttcttggcag ataggaaggt ggctgtaatc cctgtaatca tattggcacc gctggtattg
    15901 gtcgtgattt ctccaaaggc ttcttggagg ataccaacag tgagtgcggt ggtcagagtc
    15961 ttaccgttag tacctgtcac aacaacgact tcataatcct tagccaaacc atctaaaata
    16021 tgtttatcga atttgagggc aatttttcca ggtagggtag taccacggcc tagttttttc
    16081 aaaatgaact gggaactttt tcctgctaga atacccaaag ctgtatttat tttcatggga
    16141 ctattttatc ataaaggttg gggattttct agaatggagt gtgttggaac gtgatatatt
    16201 cctgcagtaa atctgtaata tttttaacag gaaggtcact attttctaaa gaaaagggga
    16261 aaaatatggt ataatgagta cattactatg tatgagttag aaagtatgag aatgatatgt
    16321 taaacctaaa ccagttactg gatacagggt attggtcgag tttgatagcc agtccttgga
    16381 ccgcactgtt gcacctaatt gatattagta tagttgttta tttgatttat aattttagta
    16441 aggctattgc aggtactaaa attatgacat tgattcgagg ggtttttctc ttcattattg
    16501 tccagattat agcgagttta ttcggtctgc agacgattgc ttggctgatt aatcaagtaa
    16561 tcacgtatgg ggttattgca gcagtagtta tttttgcacc agagttgcgt gctatgctcg
    16621 aaaaactcgg taggacaact cagatttttg ggacaaatac agtgagtgca gaagaaaagt
    16681 taattgttgc ttttctcaaa tcggttgctt atatgtctcc gcgtaaaatt ggtgcacttg
    16741 tggcagtaga acaagcccaa acattgcaag aatatagagc aacagggatt ccactcaatg
    16801 cggatatttc tcaggaattg ttaataaata ttttcatccc gaatacaccg ctacatgatg
    16861 gggctgtaat tgtcaaggaa gacaaggtag ctgttgcttg tgcttatttg cctctgtcag
    16921 agagcgcagg aatttcaaag gaatttggaa cacgacatcg tgcagccatt ggcttatcgg
    16981 aggtatctga tgcgtttgta tttattgttt ctgaagaaac tggtagtatc tctattgcac
    17041 gaaacgggat atttaaacat gatttgactt tggatgagtt tgaagctgag ttgcgagcga
    17101 cttttatttc tgaaaatgta gagaagaagt ccatctggaa gcgattagga ggtggcaaac
    17161 atgaacgata agtttaaagc tgtaggtcac ttagccttat ctatttttct agcattgttg
    17221 ctttttttct atgcgacaac caccaactat aaaaattcag aatcagctgc acagaacacg
    17281 gagtcagaaa cctatgttca cacactgagc aatgtaccga ttgaaattga atatgataca
    17341 aacaagtatt ttatttctgg actttcttcg acggtggcag ttgaattaag gggctctaat
    17401 cgtgttctcc tacagagaga atcagatgag tcaactcgga cttttcaagt gactgcggat
    17461 ttgagagagt tgagtgatgg ggcacagact gtcaagctac aattgaccaa tttgccagca
    17521 ggagtgagtg cgacactagc gccagatgcg attaccgtta agattggtaa aaaagttagc
    17581 aaggcattcc cggtagctgg tagagtatat agtaatcaat tggcagaagg ttacagtctg
    17641 tctaaggttt cagtcaacgt agataccgtt aaagtgacaa cggatgaaga aacaatggct
    17701 aagattgatc gggtcgaagc tgttgcgatg gatgtgagca atctgtctga gaattatagt
    17761 ggtacagcta aattacaggc tgtagatagc gatggtaatg tcttgccagt tgtactgtct
    17821 cagacggaag caaatatgca aattatactt acaaaaacaa aataagaggt tatgaaaatg
    17881 ggtaaatatt ttgggacaga tggtgtccgt ggagaagcaa acgtagaatt aacgccagaa
    17941 ttagcgttca aattgggtcg ttttggtggt tatgtcctta gccagcatga gacggatgtt
    18001 cctcgtgtct tggtggcgcg tgacacacgt atctcaggtc aaatgttaga ggctgccttg
    18061 attgcaggac ttctatcagt agggattcat gtgtataaat tgggagtttt ggcgacaccg
    18121 ggtgttgccc acctagtcaa aactgaaaaa gcaagtgcag gtgtcatgat ttctgcaagt
    18181 cataacccag cgcaagataa cggtatcaaa ttctttgctg gtgatggttt caaattggat
    18241 gatgcattgg aagcagaaat tgaggctctt ttggatgctg aggaagacac attgccacgc
    18301 ccatctgcta aaggtctagg agatgtggta gaatatccag aaggtctacg taagtaccag
    18361 caattcttgg tgtcaactgg tacagatttg gacggtatga aagttgcact tgatacagcc
    18421 aatggtgccg cagcaacatc tgcacgtcaa atttttgtag atttgggagc agatttgaca
    18481 gtcatggctg aaaagccaga cggtttaaat atcaatgagg gcgttggctc aactcatcct
    18541 gaaaaattac aagaactggt caaggaaaca ggtagtcaaa ttggacttgc ctttgatggt
    18601 gacagtgacc gcttgattgc cgtcgatgaa aatggagatt tggtagatgg cgaccgcatc
    18661 atgtatatcg ttggtaaata ccttgctgat cgaggtctat tggccaaaaa taccattgtg
    18721 acaactgtta tgtctaacct tggtttccat aaggctttgg accgtgaagg tattgaaaaa
    18781 gctgtgaccg ccgttggtga ccgttatgtg gttgaagaaa tgcgcaagga aggctacaat
    18841 gtcggtggtg agcagtcagg acatgtgatt ctgatggatt ataacaccac aggcgacggc
    18901 cagttgaccg ctgtgcaatt gaccaaaatc atgaaagaaa ctggtaagaa gttgtcagaa
    18961 ttggcggccg aagtcactat ttacccacaa aaattggtca atattcgtgt ggaaaacagc
    19021 atgaaggaca aggcaatgga agtgcctgct attgcagcta tcattgaaaa aatggaagca
    19081 gaaatggctg gcaacggtcg tatcttggtt cgcccaagtg gcactgagcc cctcttgcgt
    19141 gtcatggcag aagcgccaac ggatgctgaa gtcgattact atgtggacac cattgccgat
    19201 gtggtccgtg cggaaatcgg tttggattaa gaaaaagaca gagactggaa ggatttccag
    19261 cctcttttgt gaggaaatat gagtatttta gaagtaaaga atttaagtca tgggtttggt
    19321 gaccgcgcaa tttttgaaaa tgtctccttc cgtctcttga aaggggaaca tatcggactt
    19381 gtcggtgcca atggagaagg gaaatcgacc ttcatgtcca tcgtgacagg tcaattgcag
    19441 ccagatgaag gtaaggttga atggtcacgt tatgtaacgg caggttattt agaccagcat
    19501 gctaagttgg aaaaaggcca gtctgttcgt gatgtcttgc gaacggcctt tgatgagttt
    19561 tttaagacag aagcccgcat caatgacatt tacatgtcca tggcagaaga gggagttgat
    19621 atggatgccc tcatggaaga gggagttgat atggatgccc tcatggaaga ggttggtgag
    19681 ctgcaagacc gtctggaaag ccgtgatttc tatacgcttg atgccaagat tgacgaagtg
    19741 gcgcgtgcgc ttggtgtcat ggattatggc atggacaagg atgttacgga gttatcaggg
    19801 ggacaacgga ctaaagttct tcttgcaaaa ttgctcttgg aaaagcctga catcttgctt
    19861 ctggatgagc cgaccaacta cttggatgca gagcatattg actggctcaa acgctacttg
    19921 cagaactatg aaaatgcctt tgtcttgatt tcgcatgaca ttcctttcct gaatgatgtg
    19981 attaacattg tctaccatgt ggaaaatcag cttttgactc gctatacagg tgattactac
    20041 caattccaag aagtacatgc tatgaagcga gctcagttgg aggcggctta tgagcgccag
    20101 cagaaggaaa tcgcagactt gcaggacttc gttaaccgta acaaggcccg tgttgcaact
    20161 cgaaatatgg ccatgtctcg tcagaagaag ctggacaaga tggagattat tgagcttcaa
    20221 gcagagaaac caaagccatc atttgatttc aaaatggctc gaactccaag tcgctttatc
    20281 ttccaaacga ctgatttgga aattggttat gaccgtgtat tgacacgtaa accgctcaat
    20341 ctgacctttg aacgcaacca gaagattgcc attgtcggtg tcaacggtat cggtaaatct
    20401 actcttctta aaagtttgct ggggattatt ccaccgcttg gtggttctgt ggagcgtggg
    20461 gagtatctgg aactaggcta tttcgagcaa gaagtagctg gtggcaatcg tcagactcct
    20521 cttgaagccg tttgggatgc ctttccagcc cttaaccagg cagaagtacg agcagccttg
    20581 gctcgttgcg gtctgacttc caagcatatc gaaagccaaa ttcaagttct ttcaggtggt
    20641 gagcaggcca aaattcgctt ctgtttgctt atgaaccgtg aaaacaatgt cttggtcctt
    20701 gacgagccga ccaatcactt ggatgtggat gccaaggacg aactcaaacg tgcccttcaa
    20761 gcttataagg gctcaattct catggtctgc cacgagcctg atttctatga aggctgggtt
    20821 gacgtatttg ggattttaat gaattgactt aagaaacaag ctatcgaaag gtagcttcag
    20881 actgaagaca aagtccttag aatttatttt ctaagggctt ttctt
//
LOCUS       DATUCG010000025        20788 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.026, whole genome shotgun sequence.
ACCESSION   DATUCG010000025 DATUCG010000000
VERSION     DATUCG010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20788
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.026"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(<1..522)
                     /locus_tag="VCA20_000751"
     CDS             complement(<1..522)
                     /locus_tag="VCA20_000751"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834602.1"
                     /translation="MKVTYRLKGKILDKSLKNISLVAVMKNFDHQNHLFKQIVSVASL
                     ELTLKKLEQVNPKSNNLDFRNPNTEKPEKKAPSVPEVDKETEAPIEGLKSKDKITVTV
                     KGGDNSEITDLDFRYYIYQGNFQRTIYAGTTLAEFLKEIAIKEAPLDNDRKPNDVILK
                     KGYRVSKWVDDKNN"
     gene            complement(482..634)
                     /locus_tag="VCA20_000752"
     CDS             complement(482..634)
                     /locus_tag="VCA20_000752"
                     /inference="COORDINATES: protein motif:HMM:TIGR01168.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEP1834603.1"
                     /translation="MNRQTEIFSLRKSKVGLASVMIALMWFGTGVGAVVKADESNLSV
                     KRKDFR"
     gene            complement(1139..1987)
                     /locus_tag="VCA20_000753"
     CDS             complement(1139..1987)
                     /locus_tag="VCA20_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEP1834604.1"
                     /translation="MTVIDGKALGVKLQAALAEKTARLKEEKGLVPGLVVILVGENPA
                     SQVYVRNKERSALAAGFKSEVVRVPDTISESDLLDLIERYNQDDEWHGILVQLPLPAH
                     ISEEKVLLAIDPDKDVDGFHPTNMGKFWSGHPVMIPSTPAGIMEMFKEYQIELEGKSA
                     LVIGRSNIVGKPMAQLLLDANATVTIAHSRTKNLPELARQADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDENGKLIGDVKHDEVSEVASYITPVPGGVGPMTITMLMEQTYEA
                     CVRSAK"
     gene            complement(2221..2955)
                     /locus_tag="VCA20_000754"
     CDS             complement(2221..2955)
                     /locus_tag="VCA20_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019805208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEP1834605.1"
                     /translation="MSNAIISIKDLHKYFGKNEVLKGIDLDIQQGQVVVIIGPSGSGK
                     STFLRTMNLLEVPTKGTVTFEGVDITDKSNDIFKMREKMGMVFQQFNLFPNMTVLDNI
                     TLSPIKTKGIAKDEAEKKAKELLEKVGLPDKANAYPQSLSGGQQQRIAIARGLAMDPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMDGGVI
                     VEDGTPEEVFEHTKEERTKDFLSKVL"
     gene            complement(2948..3637)
                     /locus_tag="VCA20_000755"
     CDS             complement(2948..3637)
                     /locus_tag="VCA20_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEP1834606.1"
                     /translation="MNFSFLPEYWAYFNYGAIVTLIIAFFSVFFGSILGVLLAFAQRS
                     KYKVLAWTANIYVWIFRGTPMIVQIMIAFAMTHFVAPTFQLGILTVDLTRLIPGIIVI
                     SMNSGAYVSETVRAGINAVPKGQLEAAYSLGIRSKQAMRYVIMPQAIKNILPALGNEF
                     VTIVKDSSLLSTIGVMELWNGAQTVQSTSYIPLTPLVFAASYYLIMTTVLTVIIQSFE
                     KKLNKGGQFHV"
     gene            complement(3763..3993)
                     /locus_tag="VCA20_000756"
     CDS             complement(3763..3993)
                     /locus_tag="VCA20_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1797 family protein"
                     /protein_id="HEP1834607.1"
                     /translation="MESHLVRIINRLEAMANDGGTLKRNFERDGIVVAEVAYSYDEEN
                     GSVFTLRDVAARETYAFDSIDLIAMEIYELLY"
     gene            4217..6445
                     /locus_tag="VCA20_000757"
     CDS             4217..6445
                     /locus_tag="VCA20_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEP1834608.1"
                     /translation="MLCKNCNINDATIHLYTNLNGKQQQVDLCHNCYQIMKTDPNNAI
                     LRGLGDLTNPNNMDPFSEFFNHLGGYPGNTPAGKNREQTPPTQAGGHNGRGGQTPPPQ
                     QPQQPNGLLEEFGINVTEIARRGDIDPVIGRDQEITRVIEILNRRTKNNPVLIGEPGV
                     GKTAVVEGLAQKIVDGDVPQKLRDKEVIRLDVVSLVQGTGIRGQFEERMQKLMEEIRN
                     RREIILFIDEIHEIVGAGSAGDGNMDAGNILKPALARGEMQLVGATTLNEYRIIEKDA
                     ALERRMQPVKVEEPSVEETITILKGIQNKYQDYHHVKYSDAAIEAAAVLSNRYIQDRF
                     LPDKAIDLLDEAGSKMNLTLNFIDPKEIDQRLIDAENRKAQATRDEDYEKAAYFRDQI
                     AKYKEMQKATISEEDIPLITEKEIEAIVEQKTNIPVGDLKEKEQSQLVNLASDLKAHV
                     IGQDEAVDKIAKAIRRNRVGLGAPNRPIGSFLFVGPTGVGKTELSKQLAIELFGSADS
                     MIRFDMSEYMEKHAVAKLVGAPPGYVGYEEAGQLTEKVRRNPYSLILLDEVEKAHPDV
                     MHMFLQVLDDGRLTDGQGRTVSFKDTIIIMTSNAGTGKVEASVGFGAAMEGRTQSVLG
                     QLSNFFTPEFMNRFDGIIEFQPLSKENLLEIVSLMLDDVNKRLSHNGISLHVTDKVKE
                     KLVDLGYDPKMGARPLRRTIQDQIEDAITDFYLEHPAEKDLRAVMSSKGTIQIKAQTK
                     TK"
     gene            6630..7091
                     /locus_tag="VCA20_000758"
     CDS             6630..7091
                     /locus_tag="VCA20_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEP1834609.1"
                     /translation="MEKIAVFGEKKAGVSYQSRFGVYAVIPDEKKEQIILVQAPNGAW
                     FLPGGEIEKGENHLIALERELMEELGFTAEIGKYFGQADEYFYSSHRDTHFYNPAYIY
                     EVTSYHQIGQPLEDFNHIAWFPVNEAIDKLKRGSHKWGIEQWKLQENSFNN"
     gene            7146..7454
                     /locus_tag="VCA20_000759"
     CDS             7146..7454
                     /locus_tag="VCA20_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEP1834610.1"
                     /translation="MKIINTTNSHPNLVQSQLANTDAFLVETYSAGNTDVIFTQAPRH
                     YELLISNKYRAVQQNEIEQIRDFFLHRKIDERTINKAAIQTIHTDRLIEMSIPIIAEV
                     "
     gene            complement(7912..10701)
                     /locus_tag="VCA20_000760"
     CDS             complement(7912..10701)
                     /locus_tag="VCA20_000760"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEP1834611.1"
                     /translation="MKLKETLNLGKTAFPMRAGLPTREPEWQKAWDEANLYARRQELN
                     EGKPAFHLHDGPPYANGNIHVGHALNHISKDIIVRSKSMSGFRAPYVPGWDTHGLPIE
                     QVLAKQGVKRKEMDLVEYLEMCRDYALSQVDKQRDDFKRLGMSGDFDNPYITLSKDYE
                     AAQIRVFGAMADKGYIYRGAKPVYWSWSSESALAEAEIEYHDIDSTSLYYANKVKDGK
                     GLLDTDSYIVVWTTTPFTVTASRGLTMGVDIDYVLVQPAGSNRKYVLAEALVDSLAAK
                     FGWESFEVISKHKGAEFEYIVTEHPWDAEVDELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGVKYDLEVAVTVNERGLMNEAAGPDFEGQFYDKVVPTVKEKLGDLLLASEVINH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDQIEATTFNPSWGKTRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMTKEVTDHVAALFEEHGSIIWWKSDAKDLLPAGF
                     THPGSPNGEFTKETDIMDVWFDSGSSWNGVMNARENLAYPADLYLEGSDQYRGWFNSS
                     LITSVAVNGHAPYKAILSQGFVLDGKGMKMSKSLGNTILPSDVEKQFGAEILRLWVTS
                     VDTSNDVRVSMDILGQVSETYRKIRNTLRFLIANTSDFNPASDAVAYEELRSVDQYLL
                     VKFNKLVAQIREAYDNYDFMGIYKSVVNFVTLDLSAFYLDFAKDVVYIDGAKSLSRRQ
                     MQTVFYDILVKITKLLTPILPHTAEEIWSYLEHEAEEFVQLAEMPEVETFANEAQLLA
                     DWENFMVFRTQAQKALEEARNAKVIGKSLEAHLTAYVSAETKSFLESLNADLAQLLIV
                     SNLTVTTETAPSTALVVDEVAFAVERAAGEVCDRCRRIDTSVAERSYGATICDHCASV
                     VEENFAEAVAQGFEA"
     gene            complement(10712..11155)
                     /locus_tag="VCA20_000761"
     CDS             complement(10712..11155)
                     /locus_tag="VCA20_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEP1834612.1"
                     /translation="MIRLELVNKDNFEAVLQVQLASEDQRRVASVEYSLAQAWLYKDS
                     GMILPYAVVSGRKVVGFAMLSIEPKDNSYYLWRLLIDKEFQNRGCGKEAIQQIIGKAK
                     ADLLCHKISINYVIGNHKMRYILEKLGFQSVGLEGQEMKMELIIK"
     gene            complement(11385..12074)
                     /locus_tag="VCA20_000762"
     CDS             complement(11385..12074)
                     /locus_tag="VCA20_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEP1834613.1"
                     /translation="MALTALELKDKTFATKFRGYDADEVDDFLDIVTRDYEDLIRKNH
                     DQELELKNLRERLAYFDEMKESLSKSVLLAQDTAEKVKVAAEDQAANIIKQADYDAAT
                     LLHEAKDKANEILRNATDNAKKVVIETEELKNQTRIFHQRLKSTVESQLSLVNSSEWE
                     EILRPTASYIQTSDEAFRDVLHKALDEELPVEEESLDYTRQLTPEEIAELTRQAAAFE
                     SGESVVLSIEE"
     gene            complement(12083..12874)
                     /locus_tag="VCA20_000763"
     CDS             complement(12083..12874)
                     /locus_tag="VCA20_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEP1834614.1"
                     /translation="MKNDKNLLQHFSREEREFVEKIMDMCQQVEDTYSYRLTQFLHPR
                     QDEIVCKISNYYQLQTFSSRDFVSTEHSRVIIAPAYYELDIKDFELTALHLSYARKFH
                     TLSHSQVLGTFLNQLGIKREYLGDILIDDERLIVFIDQKFGQIALQSITKVARVPVKG
                     KEEEWTTVRLPIGQEWRSKDVLVSSMRLDKLISVAFNLSRATANKLIEAGHVKLDYVL
                     TEQTSKLVEIGQLISVRRYGRVRLNEFLGFSKQGKIKLKLDIVKT"
     gene            complement(12877..13140)
                     /locus_tag="VCA20_000764"
     CDS             complement(12877..13140)
                     /locus_tag="VCA20_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEP1834615.1"
                     /translation="MQILIFLLLKIVEIYSYLLLAYALLSWFPSLYTSSIGRLIQWLV
                     APILKPFRRLNLQFMGLDWTVMAAMIALNMGTRLLVQLLLLLA"
     gene            complement(13145..13714)
                     /gene="sepF"
                     /locus_tag="VCA20_000765"
     CDS             complement(13145..13714)
                     /gene="sepF"
                     /locus_tag="VCA20_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEP1834616.1"
                     /translation="MALKDTFKNLFNYFEVDEVNEVEEQADAYSMPNDRPKMRVANTT
                     AASVREQQPKVETRREARSESQLQRLHERQQELMTNNNEKEIVKTTIDIKFPKRYEDA
                     PEMVNLLLDNASILIDFQYMSEQQARRCLDYLDGARSVLSGNLKKVSNTMWLLTPVNV
                     TVNIEELRNANAGTTTGAADSNFDFDIKR"
     gene            complement(13731..14402)
                     /locus_tag="VCA20_000766"
     CDS             complement(13731..14402)
                     /locus_tag="VCA20_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEP1834617.1"
                     /translation="MNFQKNKDAIFSAVAKAAEKAGRPSESVKVIAVTKYVDSSIANQ
                     LVNTGITHIGENRVDQFLEKYNALADKQLTWHLIGTLQRRKVKDIIPFVDYFHALDSV
                     KLAQEIDKRATKIVKCFLQVNVSGEESKHGFAVSEIDVGLAEISLLENIELVGLMTMA
                     PIDATDDELDEIFSKMQEIQEELATRNLPRMPFTELSMGMSGDFERAIAHGATYVRIG
                     SAFFE"
     gene            complement(14409..15638)
                     /gene="ftsZ"
                     /locus_tag="VCA20_000767"
     CDS             complement(14409..15638)
                     /gene="ftsZ"
                     /locus_tag="VCA20_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002263818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEP1834618.1"
                     /translation="MAFSFEAAASHGAVIKVIGVGGGGGNAINRMIEEGVAGVEFIAA
                     NTDVQALSSSKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEEALTNVLTGADMVFI
                     TAGMGGGSGTGAAPVIARIAKNLGALTVAVVTRPFGFEGNKRGNFAIEGIEGLREQVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MENKGNALMGIGIGTGEDRVIEAARKAIYSPLLETTIDGAEDVIVNVTGGYDMTLTEA
                     EDASEIVNQAAGQGVNIWLGTSIDETMKDEIRVTVVATGVRQDTADKPARHRTEAVSP
                     RPSQRFDHSVASAPTRGVAQHTEAPKASAFGEWDLRRENLIRPTDTEPTSTVSVEKFT
                     MDQDEDELDTPPFFRNR"
     gene            complement(15664..17043)
                     /gene="ftsA"
                     /locus_tag="VCA20_000768"
     CDS             complement(15664..17043)
                     /gene="ftsA"
                     /locus_tag="VCA20_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEP1834619.1"
                     /translation="MARNGFFTGLDIGTSSVKVLVAEYIDNEMNVIGVSNVKSAGVKD
                     GIIVNIEVAAGAIKKAIEQAEEKSGIRIEKVNVGLPANLLQIEPTQGMIPVTTDSQEI
                     TELDVENVVKSALTKSMTPEREVISFIPEEFTVDGFQGIKDPRGMMGIRLEMRGMLYT
                     GPRTILHNLRKTVERAGVQVENIVISPLALTRSVLNEGEREFGATVIDLGGGQTTVAV
                     MRGQELQYTNIYQEGGDYITNDISKVLTTSKSIAENLKYNYGIAYPQDASDKEKFTVE
                     VIGENAPVEVTERYLSEVIAARLRQIFDRVKQDLERTRALDLPGGIVIVGGGAILPGI
                     TELAQEVFGVNTKLFVPNQIGIRNPAFASVISFVEYVGELEDVENIAQHAVNGETTLR
                     HKPVEIPVARPRISQPIQREIVNELNTVDQVEEQVAPTYHDEEEEVRPAAKGNITDRI
                     RGLFGSMFE"
     gene            complement(17195..18289)
                     /locus_tag="VCA20_000769"
     CDS             complement(17195..18289)
                     /locus_tag="VCA20_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HEP1834620.1"
                     /translation="MTEKDSNVEESVLEVEQASQVELDSEQISPAEKESVLAEEKGLS
                     TDVYTDVDIPEMTAPDDEKSAFFEQWKARHQAYLAHKDAAEIQAVDEGQTEQENPEAK
                     KSKRVLFQGIERKTESLESKKETGEKVQTLKVDIPSKVVWKAIPVLVTSLLLAALALY
                     FISPTSKKKQIEVVGNERLTAEQVENYSLISPDDYNVTIALHADAYAKNIKKNSSSVE
                     TATIKFQFPATFTIHIKEYAIIGYIQQQSQWYPVLSSGEIGGEPISQDSLPEDYTTIN
                     LSDKELIKELAIELGKIDAGIRSAIQTINLTPSKVTADLLTFNMRDGNTVLVPLSEIS
                     QKVSYYTKIAAEVTVPITIDMEVGIYRYAS"
     gene            complement(18295..19359)
                     /locus_tag="VCA20_000770"
     CDS             complement(18295..19359)
                     /locus_tag="VCA20_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEP1834621.1"
                     /translation="MKKIVFTGGGTVGHVTLNLLLIPRFLEEGWEVHYIGDGNGIEYE
                     QVVKSGLDVHFHSISTGKLRRYFSFQNMLDVFKVGFGVLQSLAIIAKIRPQALFSKGG
                     FVSVPPVIAANLLRVPVFIHESDLTMGLANKIAYKFATTMYSTFEQPASLTKVKHVGA
                     VTKVGQTNDKVTPIQLPEILSHFDKSLPTLLFVGGSGGAKVFNDLISQNSAALTERFN
                     IINLTGDSSFNQLDKNLYRVDYVTELYQPLMDLADVVITRGGSNTLFELIAMQQLHLI
                     VPLGRQASRGDQIENALYAEKKGYSKQIDESQLTFASLLVEVDELLKHKEFYIQNMTN
                     SNEIQSVDSFYNLLREDMGR"
     gene            complement(19362..20711)
                     /locus_tag="VCA20_000771"
     CDS             complement(19362..20711)
                     /locus_tag="VCA20_000771"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEP1834622.1"
                     /translation="MKMIDIVKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFE
                     ENPTAQSLLEDGIRVICGSHPLELLDEDFALMVKNPGIRYDNPMVEKAIGKGIPVWTE
                     VELAYLVSDAPIVGITGSNGKTTTTTMIAEVLNVGNKPAKLCGNIGYPASSVAQTATA
                     EDTLVMELSSFQLMGTESFHPHIAVVTNLIASHIDYHGTFEDYVAAKWMIQRQMTAED
                     FVVLNFNQKETKELAGQTKATVVPFSTLEVVDGAYLADGKLYFKGEYIMDADQIGVPG
                     SHNVENALATIVVAKLLGIDHQAIQESLSAFGGVKHRLQFVGEINGVSFYNDSKSTNI
                     LATQKALSGFDNSKVILIAGGLDRGNEFDELVPDLVGLKKMVILGQSAPRVQRAADKA
                     EVETIEALDIADATRKAFAIAEKGDIVLLSPANASWDMYANFEVRGDVFLQTFEELK"
BASE COUNT         6104 a         4819 c         3676 g         6189 t
ORIGIN      
        1 attattctta tcatctaccc atttcgaaac cctgtaacct ttttttagaa taacatcatt
       61 cggttttcga tcattatcaa gcggagcttc tttaatagca atttctttca aaaattctgc
      121 taacgttgtc cctgcataaa ttgttctttg gaaattacct tgataaatat aataacggaa
      181 atccaaatcc gtaatttctg aattgtcccc tcctttaact gtaacggtaa tcttatcttt
      241 tgattttaaa ccttctatag gagcttcagt ttctttgtct acctcaggaa ctgaaggtgc
      301 tttcttttct ggtttctcag tgtttggatt tctaaaatct aaattattcg actttggatt
      361 tacctgctct aacttcttaa gagtcaattc taaactagca accgaaacga tttgcttaaa
      421 tagatgattc tgatgatcaa aatttttcat tactgcaact agtgaaatat ttttcagtga
      481 cttatctaaa atctttcctt ttaaccgata agttactttc atcagctttt acaactgctc
      541 caacacctgt tccaaaccac ataagcgcaa tcataacaga agctaatcca accttactct
      601 ttctcaacga aaaaatttca gtctgtctgt tcattttttc tccttgtttt ctatcattta
      661 atactcaaca cacacagtgc ctactctaat tatagaaaac caatctcaca tagtattatg
      721 tttcgtttgt ttttatctaa tctgcccata ctcattccta agaatatgcc tcaaaacgaa
      781 gctgggttgg gcaaaaagcc caggactgct actcagagtt cgtgtcaaca tctcagcgca
      841 gtggttgatt gggtttaaca gtccactgga ctgttaaagg ttggagataa gatttgcgaa
      901 gcaaatctta tctccaacct caaactgtct cccagacagt ttgagctgtg cgggggtggg
      961 agtgaaacag tctggggata gactgtttca gctcaacaac ttaaaacgaa aaattgttga
     1021 cgaactcttt ttatcttggt cgagttcttt cccactcccg taccacttct ctgatttcgt
     1081 cttcccactc ccaaaataaa aaataccagc tacaccacat agctgatatt tcagataatt
     1141 attttgcact tctgacacaa gcttcatagg tctgttccat caacatggta atagtcatcg
     1201 gaccaacacc acctggaact ggggtaatat aacttgccac ttctgatacc tcatcgtgtt
     1261 tgacatcacc gattagcttg ccattttcat cacgattcat accaacatca atcaccacgg
     1321 cacctggctt aacaaattcc ttggtcacaa aatgaccacg accaatggca acaacaagaa
     1381 tatcggcctg acgagcaagc tctggcaagt ttttggtacg agaatgcgca atcgttacag
     1441 tagcattggc atccaataac agctgtgcca tcggtttacc aacaatatta gaacgaccaa
     1501 taactaaagc tgacttccct tccagctcaa tctgatattc cttgaacatt tccataattc
     1561 ctgctggggt agatggaatc ataacaggat gaccggacca gaatttcccc atgttagtcg
     1621 gatgaaaacc atccacatcc ttatctgggt caatggccaa caagaccttc tcctcgctga
     1681 tgtgggctgg taagggcaat tgcaccaaaa taccatgcca ttcatcatcc tgattatagc
     1741 gctcaatcaa atccaacaag tctgactcag aaatcgtatc tggaacgcga acaacctcac
     1801 tcttgaatcc agcagccaga gctgaccgct ccttattccg tacataaacc tgactagcag
     1861 gattttcgcc aactaaaata actacaagac ctggtaccaa acccttttct tcttttaatc
     1921 gagcagtttt ctccgccaag gcagcctgca attttacacc tagtgctttc ccatcaatga
     1981 ctgtcatacc tacactcctt ttgttttttc tattataccc taaaattctc cgactgacta
     2041 tacttgtaca aagtagacta tagcatatag caaaaaactc cgaacaatgt cggagtcgta
     2101 cttatttgtt catttacagt catttgaatt aactttgata catcgtcact ttcgacttgc
     2161 cgtactttag tacagcctgc gcctcagttc cttgtctgaa agctaattca ttcgactata
     2221 ttacaagacc ttagacaaga aatccttggt ccgttcttcc ttggtatgtt caaagacttc
     2281 ttcaggcgtt ccatcctcca cgatgacacc gccatccata aagataaccc tgtcagctac
     2341 ctcacgcgca aatcccatct catgagtcac gataaccatg gtcatccccg acttggctaa
     2401 atcctgcatt acagcaagaa cttcaccaac catttcagga tctagtgcag aggtcggttc
     2461 atcaaaaagt aggacatctg ggtccatggc cagaccacgt gcaatagcga ttcgttgttg
     2521 ctgaccacct gaaaggcttt gtggataggc atttgcctta tctggcaatc ctaccttttc
     2581 aagtaattcc ttagccttct tctcagcctc atcctttgca attccctttg tcttaatagg
     2641 tgataaagta atattatcta ataccgtcat atttggaaaa agattgaact gttgaaaaac
     2701 cattcccatc ttttcacgca tcttgaaaat atcatttgac ttgtcagtaa tatcaacacc
     2761 ttcaaatgta acagttccct tggttggcac ttctaagagg ttcattgtac gtaagaaagt
     2821 cgatttccct gaccctgatg gaccgataat aacgacaacc tgaccttgtt gaatatctaa
     2881 atcaattcct tttagaacct cattctttcc gaagtactta tgtaaatcct tgatagaaat
     2941 aatcgcatta gacatggaac tgccctccct tgttcaactt tttctcaaac gactgaataa
     3001 tcactgttag caccgttgtc ataattagat aatagctcgc tgcaaataca agtggtgtta
     3061 aaggaatata agaagtggat tgaactgtct gagcaccgtt ccataattcc atcacaccga
     3121 tggttgacaa aagtgaacta tccttaacaa tagtcacaaa ttcatttccc aaggctggaa
     3181 gaatattctt gatagcctgt ggcatgataa cgtaacgcat cgcctgcttt gatctaatac
     3241 caagcgaata agctgcttcc agctgtcctt ttggtacagc attaatacct gcacgaacag
     3301 tttcagatac ataggcaccc gagttcatcg aaataacaat gatacctgga atcaagcgcg
     3361 tcaaatcaac cgtcaaaatt cctagctgaa aagttggtgc tacaaaatgt gtcatcgcaa
     3421 aagcaatcat aatctggaca atcatcggtg tcccacggaa aatccagaca tagatattgg
     3481 cagtccaagc taaaacttta tacttactac gctgagcaaa ggcaagcaac acacctaaaa
     3541 tactaccaaa aaatactgag aaaaatgcaa taatgagggt aacaattgcc ccataattaa
     3601 aataagccca atactcaggc aaaaaagaaa aattcattca tcttactcca attctgtcat
     3661 ttagagtcta ttataccatt tttgaatatt tatgcaacac ttttgtttat aatattcaat
     3721 ttaaacaaga aaaaagcacc cttaggtgcc tctcacttct tcttagtaca atagctcata
     3781 gatttccatt gcaatcaagt cgatgctgtc aaacgcgtac gtttcacgag cagcaacatc
     3841 acgcaaggta aatactgaac cattttcctc gtcatagcta taagccactt ctgcaacaac
     3901 aataccatca cgttcgaaat tacgtttcaa cgtaccgccg tcattagcca tagcttccag
     3961 acgattgata attctcacca aatgtgattc catattcgtt ctcctaatcc tttttatagg
     4021 aatattttac cacatattgc caaaaaagtc ttattttttc cctattttct tcctatcgcc
     4081 ctatttttca tagaaagttt ttccaagcgt agcagtttct tgatttttct atgaatcttg
     4141 ttataataaa gctagaactg accttttttg actatttaac aataggtcac attttttcac
     4201 agaaagaggt agaattatgc tctgcaaaaa ttgtaatatc aacgacgcaa cgatccatct
     4261 ctacaccaac ttaaacggta aacagcagca ggtagacctc tgtcacaact gctaccaaat
     4321 catgaaaaca gaccctaata atgccatcct acgtgggttg ggagatttaa caaatcctaa
     4381 caatatggat ccctttagtg aattcttcaa tcacctaggt ggctatcctg gcaatacccc
     4441 tgctggaaaa aatcgcgaac agactcctcc cactcaagcc ggtgggcata atggacgtgg
     4501 tggacagacc cctcctccgc aacaacccca acaaccaaat gggctccttg aagaatttgg
     4561 tatcaacgtt accgaaattg cccgtcgtgg ggatattgat ccagtaatcg gtcgcgatca
     4621 agaaattacc cgtgtcatcg aaattctcaa ccgtcgtaca aaaaacaacc ctgttcttat
     4681 cggtgagcct ggtgtaggta aaacagcggt tgttgaaggc ttggctcaaa aaattgtcga
     4741 tggcgatgtc ccacagaaat tacgagacaa ggaagtcatt cgtctggatg ttgtcagcct
     4801 cgtacaagga acaggtatcc gtggacaatt tgaagaacgc atgcaaaaac tcatggaaga
     4861 aatccgtaat cgtcgcgaaa tcattctttt tattgacgag attcacgaaa ttgtcggtgc
     4921 tggatctgca ggcgatggta acatggatgc aggcaatatc ctcaaaccag ccctcgcacg
     4981 tggtgaaatg caactagtcg gtgcaacaac gctcaacgag taccgtatta tcgaaaaaga
     5041 tgccgcccta gaacgccgta tgcaacctgt aaaagttgaa gaaccaagtg tcgaagaaac
     5101 cattaccatc ctcaaaggta tccaaaataa ataccaggat taccatcatg ttaaatactc
     5161 tgatgctgct atcgaagctg ccgcagtact gtccaaccgt tacattcaag accgtttcct
     5221 cccagacaaa gctattgacc ttctggatga agctggttca aaaatgaatt tgaccctcaa
     5281 ctttattgat ccaaaggaaa ttgaccaacg cctgattgat gccgaaaatc gtaaagcaca
     5341 agctacacgg gatgaggact atgaaaaagc ggcctatttc cgtgaccaaa ttgctaagta
     5401 caaggagatg caaaaggcaa ctatcagcga ggaagatatt ccactcatta ctgaaaaaga
     5461 aattgaagcc atcgttgagc aaaaaacaaa tatcccagtt ggtgatttaa aagaaaagga
     5521 acagtcccag cttgtgaacc ttgctagtga cttaaaagcc catgttatcg gacaagatga
     5581 agccgttgac aagattgcca aggccattcg ccgtaaccgt gtcggtctcg gtgctccaaa
     5641 tcgtcccatt ggatccttcc tctttgtcgg accaaccggt gtcggtaaaa cagagctgtc
     5701 caagcaacta gcgattgaac tcttcggttc agctgatagt atgattcgct tcgatatgtc
     5761 cgaatatatg gagaaacacg ccgtagccaa actggtcggt gcccctccag gttatgtagg
     5821 ctacgaggaa gccggtcagc taactgaaaa agtccgccgc aatccttact cactcatcct
     5881 ccttgacgaa gttgaaaaag cccacccaga tgtcatgcac atgttccttc aggtcttaga
     5941 cgatgggcgt ctgacagatg gacaagggcg gacagtcagc ttcaaagata ccatcatcat
     6001 catgacatca aatgctggta ctggaaaagt tgaagcaagt gtcggttttg gtgcggctat
     6061 ggaagggcgg actcaatcag tccttggcca acttagcaat ttcttcacac ctgaatttat
     6121 gaaccgattt gacggtatta ttgagttcca accactgagc aaggaaaacc tacttgaaat
     6181 cgtcagcctc atgctagacg atgtaaacaa acgcctttct cacaatggca tcagcctcca
     6241 tgtaacagat aaagttaagg agaaattagt ggaccttggc tacgatccaa agatgggagc
     6301 tcgtccacta cgcagaacca tccaagatca gattgaagat gctatcaccg acttctactt
     6361 agaacatcca gcagaaaaag acctacgtgc tgtcatgtca agtaaaggta caattcaaat
     6421 caaggcacaa acaaagacaa aataagaaaa tagagctgct ccctacgagt agcctatttt
     6481 actgttttga tactctattt ttctaaattc ttccccatct ccatcctata aaaaacttta
     6541 ctttgtaaag cctatcacaa ggaaaaataa taaaaaagtg gtataatgta tacaactcat
     6601 tccaattatg aaagaggata gaattgccta tggagaaaat tgctgtattt ggagaaaaga
     6661 aggctggcgt cagctaccag agtcgctttg gtgtttacgc agttatccca gatgaaaaga
     6721 aagaacagat tatactcgtt caagcaccaa acggagcttg gtttcttcct ggtggagaaa
     6781 tcgaaaaggg agaaaatcac ctcattgctt tggagcgcga attgatggaa gagcttggat
     6841 tcaccgctga aatcggaaaa tactttggtc aggctgacga atacttctac tctagccacc
     6901 gcgataccca tttctataat ccagcctaca tctatgaagt aactagctat catcaaatag
     6961 gacaaccact agaagatttc aaccatattg cttggttccc agtcaatgaa gctatcgata
     7021 aactcaaacg tggtagtcac aaatggggca ttgagcaatg gaaattgcaa gaaaattcat
     7081 tcaataacta gtaaaaacac tctaatcgtg ctataatgta gccaaagaga tagaggaggt
     7141 tccacatgaa aatcattaac acgacaaata gtcatcccaa tcttgtccaa agccagttgg
     7201 ctaatactga tgctttccta gtagagactt attctgcagg taatacagac gttattttca
     7261 cacaggcacc ccgtcattat gaactcttga ttagtaataa ataccgtgca gtacaacaaa
     7321 atgaaatcga gcaaattcgt gatttcttcc ttcatcgaaa aattgatgaa cgaactatta
     7381 acaaggctgc cattcaaacg attcacacag accgtttgat tgaaatgtct attcctatta
     7441 tcgcagaagt gtaagaagcc agatggcttc tttcagattg aagaaaaagt ccattttgga
     7501 caattttctt caatcttttt tctttatgtt caaaataaaa aaactctcag aaacgctgaa
     7561 atatcaatgt ttctaagagt ttaatcactt gctatctttc gcaaacttct tcaatttttt
     7621 atatttttag gagtttgtct acgttctgaa gaggctcctc gcttctcaga gttcgtgtca
     7681 acatctcagc gcagtggttg attggcagat ttgttcgtgt ttcacactcc aaatctgacc
     7741 taatcaactg tgcggggggg tgggaagacg aactcttttt aaacttgtcg agttctttcc
     7801 cactcccttt tcactcatca aggctcttga caacggataa ttttgatttt cgaagagtat
     7861 tacaaaaaaa gcctatcgct agatagactt taacaaatgt attgtgattg cttaggcttc
     7921 aaaaccttga gctactgctt cagcaaagtt ttcttccact acgcttgcac agtggtcaca
     7981 gattgttgcg ccatagctgc gctctgcaac actagtatca atacggcggc aacggtcaca
     8041 cacctcaccg gctgcacgtt ctacagcaaa ggcaacttca tcaaccacaa gcgctgttga
     8101 tggggcagtt tcagttgtta ctgtcaagtt tgaaacgatg agcaactgag caaggtctgc
     8161 attcaagctc tccaagaagg acttggtttc agctgagaca taggcagtca aatgagcttc
     8221 aagtgactta ccaatcactt tggcattgcg tgcttcttcc aaggcttttt gtgcctgagt
     8281 acggaagacc ataaagtttt cccaatcagc taaaagctgc gcttcattag caaaggtttc
     8341 cacctctggc atttccgcca attgtacaaa ctcttctgct tcgtgttcca agtatgacca
     8401 gatttcttct gctgtatgtg gcaagattgg ggtcaagagc ttggtaattt tcaccaagat
     8461 gtcatagaag acagtttgca tctgacgacg agaaagtgat ttagcaccat caatgtagac
     8521 gacatcttta gcaaaatcga ggtagaaggc tgacaaatca agtgttacaa agtttactac
     8581 agatttgtag atgcccatga aatcataatt gtcgtaggct tcacggattt gagctaccaa
     8641 tttattgaat ttcaccaaga gatactggtc aacagaacga agctcttcgt atgccaccgc
     8701 atcacttgca ggattgaaat cagaagtgtt ggcaatcaag aagcgaaggg tattacggat
     8761 tttacggtag gtttcagaaa cctgtccaag aatatccata gacacgcgta cgtcgtttga
     8821 agtatcaacg gaagttaccc acagacgcaa gatttctgca ccaaattgtt tctcaacatc
     8881 acttggaagg attgtattcc ccaatgattt agacatcttc atccccttac cgtcaaggac
     8941 aaacccttgt gataagatag ctttgtatgg ggcatgacca tttacggcaa ctgaagtaat
     9001 caaggatgag ttaaaccaac cacggtattg gtcagaacct tctaggtaga ggtctgctgg
     9061 gtaggcaaga ttctcacgag cgttcatgac accattccaa gatgaaccag agtcaaacca
     9121 tacgtccatg atgtctgttt ccttggtaaa ttcgccattt ggtgaacctg gatgagtgaa
     9181 accagctggc aaaagatctt ttgcatcaga tttccaccag atgatagaac catgttcttc
     9241 aaagagagct gcaacgtggt ctgtcacttc cttggtcata atggctgtac catcttctgc
     9301 atagaagatt ggaagcggca caccccaagc acgttgacga gagatgaccc agtcaccacg
     9361 gtcacggatc atgttgtaaa gacgcgtttt accccaagat ggattgaaag ttgttgcttc
     9421 gatttggtcc aagatttcct gacggaattt agaaacagag gcaaaccact gtggcactgc
     9481 acgccagatg attggcttct tggttctcca gtcaaatggg taggagtgat tgatcacttc
     9541 actagcaagg agcaagtcgc ctaatttttc cttaaccgtt ggtacaacct tatcatagaa
     9601 ttgcccttca aaatcaggac ccgcagcttc attcatcaag ccacgttcgt tgacagttac
     9661 cgcaacttcc aagtcatatt tgacaccgac attgtagtca tcctcaccaa aacctggagc
     9721 cgtatggacg atacctgtac cagaatccgt tgtgacatgg tcacccaaga taaccaattc
     9781 atcaacttca gcatcccatg ggtgttcggt cacaatgtat tcaaattcag cacccttgtg
     9841 ttttgaaatc acttcaaagg attcccaacc aaatttagct gccaagctgt cgaccaaggc
     9901 ttctgccaag acatacttac ggttagaacc agctggttga accaagacat agtcaatgtc
     9961 tactcccatg gtcaaaccgc gagatgccgt tactgtaaat ggagtcgttg tccagacaac
    10021 gatgtagcta tctgtatcca agagcccctt gccatctttg accttgttgg catagtaaag
    10081 agatgttgag tcaatgtcgt ggtattcgat ttcagcttct gcaagggcag attcagatga
    10141 ccatgaccag tagacaggct tggcaccacg gtaaatatag cccttgtctg ccatggcacc
    10201 aaatacacgg atttgagctg cttcgtaatc tttagacaaa gtgatgtaag gattatcaaa
    10261 atcgccagac attcctaaac gtttaaaatc atcacgttgc ttgtcaacct gactaagcgc
    10321 ataatcacga cacatttcaa ggtattcaac caagtccatt tccttgcgtt tgacaccttg
    10381 ttttgccaag acttgctcaa taggaaggcc gtgcgtatcc caacctggca cataaggtgc
    10441 acggaagcct gacatagact tagaacggac aataatatct tttgaaatat gattcaaggc
    10501 atgcccaacg tggatatttc cgtttgcgta tggaggccca tcgtggaggt ggaaggcagg
    10561 tttgccttca ttgagttctt ggcgacgagc atacaagttt gcttcatccc aggccttttg
    10621 ccattctggc tcacgtgttg gtaagcctgc acgcattggg aaggctgtct tacctaaatt
    10681 gagtgtttct tttagtttca tcataactcc tttattttat aattaattcc attttcattt
    10741 cttggccttc taaaccaacg gattgaaaac caagtttttc taaaatgtac cgcattttat
    10801 gattgccaat gacataattt atcgaaattt tatgacaaag cagatccgcc ttcgcctttc
    10861 caattatttg ctggatggct tcttttccgc atccacgatt ttgaaattcc ttatcaatca
    10921 acaagcgcca taaataataa ctattatctt tgggttcaat agataacatt gcaaaaccaa
    10981 caacttttct gccagatacc acagcatatg gtagaatcat ccccgaatct ttatataacc
    11041 aagcttgggc taaagaatat tcaaccgatg ccactcggcg ttggtcctca gatgcaagtt
    11101 gaacttgtaa cactgcttca aagttatcct tgtttactaa ttctaatcgt atcataaaaa
    11161 taaaaaactc ccgccaaata aggacgaaag ttcgtggtac caccttagtt tagatagagt
    11221 ttcctctatc cctctatgtt cgtaacgtga acggacgtct ctccttacta ttttcaggaa
    11281 aaatcaaaca aagtgatgat ccatagaaag agattgtagg gcttccacca tctcctactc
    11341 gcttaaaata tttctaacga ccttgtcttt gcttatgatt tctattattc ttctattgaa
    11401 agtactacag attctccact ctcaaaagct gctgcctgac gagttaattc tgcaatctct
    11461 tctggtgtca attgacgtgt ataatccaaa ctttcttctt caacaggtaa ttcttcatcc
    11521 aaagccttat gaagaacatc acgaaaggct tcgtcacttg tttgaatgta acttgctgtt
    11581 gggcggagga tttcctccca ttcagatgaa ttaaccagtg ataattggct ttctactgtt
    11641 gatttcagac gctgatggaa aatacgtgtc tggtttttta attcctcagt ttcaatgaca
    11701 acttttttcg cgttgtcagt cgcattacga agaatttcat ttgccttatc tttagcttca
    11761 tgtaacaatg ttgccgcatc atagtcagct tgtttaataa tatttgccgc ttgatcttcg
    11821 gccgcaacct ttactttctc agctgtatct tgagctaaaa gaactgattt actcaatgat
    11881 tctttcatct catcaaaata agccaaacgt tcccgcaaat ttttcaattc taactcttgg
    11941 tcatgatttt tacgaattaa atcctcataa tcacgcgtta caatgtccaa aaagtcatca
    12001 acttcatctg cgtcgtatcc tctaaatttt gtagcaaagg ttttatcttt taattctaat
    12061 gctgtaagtg ccatctttcc tcctaagttt taacaatgtc taactttaat tttatcttcc
    12121 cttgcttaga aaaacctaaa aattcattta ggcgaactct accatatctc ctaacactaa
    12181 ttaattgccc tatttcaact aatttactgg tttgttctgt cagaacataa tccaacttca
    12241 catgtccagc ctcaatcaac ttatttgcag ttgcccttga aagattaaat gcgaccgaaa
    12301 tcaatttatc caatctcata ctagaaacca acacatcctt tgaacgccat tcctgtccaa
    12361 tcggtagtcg aacagtcgtc cattcttctt cctttccctt aacagggaca cgagcaactt
    12421 tagtgataga ttgtaaggca atctgcccaa atttctgatc gataaagaca atcaatcgtt
    12481 catcatcaat caaaatatca cccaaatact cccttttaat ccctagttga ttgagaaaag
    12541 ttccaagaac ttgtgaatgg gataaggtgt gaaattttct agcataggac aaatgaagag
    12601 cggttaactc aaagtccttg atgtccaact cataataagc cggggcaata ataacccgcg
    12661 aatgttcagt cgaaacaaag tcccgactag aaaacgtttg taactggtag tagttagaaa
    12721 ttttgcatac aatttcatct tgtctaggat gtaaaaactg agttaatctg tatgaatacg
    12781 tatcttcaac ttgctgacac atatccatta ttttctctac aaactctctt tcttccctag
    12841 aaaaatgttg caataagttt ttgtcatttt tcataactat gctaacagta gcaataattg
    12901 aaccaaaagg cgagttccca tattaagcgc aatcatggct gccattacgg tccaatccaa
    12961 ccccataaac tgcagattta accgcctaaa gggttttaaa atcggtgcta ccagccattg
    13021 aatcaaacga ccaatcgagc ttgtgtatag gctaggaaac caacttagca aagcgtatgc
    13081 caataagaga taagaataaa tctctacaat ttttaatagt aaaaaaatca aaatttgcat
    13141 agcgttaccg ttttatatca aaatcaaaat ttgaatcagc cgcaccagta gttgtaccag
    13201 cattagcatt gcgcaattct tcaatattga ctgtgacatt tactggagtt aatagccaca
    13261 tggtatttga gacctttttt aaatttccag ataatactga acgagcacca tccaagtagt
    13321 ctaggcaacg tcttgcttgt tgctccgaca tatattgaaa atcaatgaga atactcgcat
    13381 tgtctaataa aagatttacc atctctggtg cgtcttcata acgttttgga aacttaatat
    13441 caattgtagt tttgacaatc tctttttcgt tattattagt catcaattct tgctgccttt
    13501 catgtaggcg ttgaagttga ctttctgaac gtgcttcccg acgcgtctct actttgggct
    13561 gctgttcgcg cacagatgca gctgtagtat tggcaacacg cattttagga cgatcattag
    13621 gcattgaata ggcatctgcc tgttcttcaa cttcattgac ttcgtcaact tcaaagtaat
    13681 taaataagtt cttaaatgta tcttttaatg ccatattttc ctcctctcca ttattcaaag
    13741 aatgctgagc caattctcac atatgtagct ccgtgagcaa tggcgcgttc aaagtcccca
    13801 ctcattccca tacttaactc tgtaaagggc atacgtggta agttccttgt agctagttct
    13861 tcttggattt cctgcatttt agaaaaaatc tcgtctaatt catcatctgt agcatcaatc
    13921 ggtgccatcg tcatcaatcc aacaagttca atgttctcca ataaactaat ttcagcaagt
    13981 cccacatcaa tctcagatac tgcaaagcca tgcttactct cctcaccgga aacgttgact
    14041 tggaggaaac acttgactat tttagtcgca cgtttatcta tttcctgagc tagtttgaca
    14101 gagtctaggg cgtggaaata atcaacaaat ggaataatgt ccttgacctt tcttctctgt
    14161 aaagtcccta tcaaatgcca ggtcaattgt ttgtctgcta gggcattata cttttctaag
    14221 aactgatcga ctcgattttc tccaatatgg gtaatcccag tattcactag ctgattagca
    14281 atagagctgt caacatattt ggtgacagct attaccttta ctgattcact cggacgacct
    14341 gccttctcag cagcttttgc cactgctgaa aagatagcat ccttattctt ttgaaaattc
    14401 attttaattt agcgattacg gaagaatggt ggtgtatcca attcatcctc atcttgatcc
    14461 attgtaaatt tttcgactga aacagtggat gttggttctg tgtcagttgg acgaatcaag
    14521 ttttcgcgac gcaaatccca ctctccgaag gcagaagctt tcggcgcttc agtgtgttgt
    14581 gcaactccac gagttggtgc tgatgctact gaatgatcaa agcgttgaga agggcgtggt
    14641 gaaacagctt ccgtgcgatg gcgggctggt ttatcagcag tatcttgacg aacacccgta
    14701 gccacaacgg tcacacgaat ctcatctttc attgtttcat caatagatgt acccaaccaa
    14761 atgttaacac cttgacccgc agcctgatta acgatttctg aagcatcttc tgcttctgtc
    14821 agagtcatgt catagccacc tgtaacattg acaatgacat cctctgcacc atcaattgtt
    14881 gtttcaagaa gaggagaata aatcgcttta cgagccgcct caatcactcg gtcctcacca
    14941 gtaccgatac cgatacccat caaggcgttt cctttgtttt ccataactgt cttcacatct
    15001 gcaaagtcca agttaatcaa accaggattt gtaatcaagt ctgtaattcc ttgaacacct
    15061 tgacgaagaa cattatcagc ttcgcttagt gcttctaaaa gcggtgtttt cttatcaaca
    15121 atttcaagaa ggttgttgtt tgaaataatt aaaagtgtat ctacttgttc gcggagacct
    15181 tcaattccct caatcgcaaa gtttccacgt ttatttcctt caaaaccaaa cggacgagtc
    15241 acaactgcga cagtcaacgc acccaagttt ttagcaatac gtgcaataac aggagccgca
    15301 ccagtccctg agccaccgcc cataccagca gtgatgaaga ccatatctgc accagttaat
    15361 acattagtca atgcttcttc gctttcctca gcagctttac gaccaacctc aggttgaccc
    15421 ccagcaccca aaccacgagt caatttagga cctagttgga taactgtttc agctttagag
    15481 ctacttagcg cttgtacatc tgtgtttgct gcaatgaact caaccccagc aacaccttct
    15541 tcaatcatac ggttaatcgc gttcccgcca ccaccgccga caccgattac tttaatgaca
    15601 gcaccatgac tagctgctgc ttcaaatgaa aatgccatag tttaaatcac cttttctttt
    15661 ctcttattca aacatgctgc caaacaaacc acgaatgcga tctgtgatgt ttcccttagc
    15721 agctggacga acttcttctt cctcatcatg gtaagtagga gcaacttgtt cctctacctg
    15781 atcaactgtg ttcaattcgt tcacgatttc tctttgaatt ggctgactaa tccgaggacg
    15841 agcaacagga atttcaactg gcttatgtct caaggtagtt tcaccattta ctgcatgttg
    15901 agcaatattt tcaacatctt ccaactcacc aacatactca acaaagctaa tcacacttgc
    15961 aaatgcagga ttgcggatac caatttgatt tggaacaaaa agtttagtgt taacaccaaa
    16021 cacttcttgg gccaattctg taattcctgg taagatagca ccaccaccta caataacaat
    16081 gcctcctggt aaatctagag cacgtgtacg ctccaaatct tgtttcacac ggtcaaaaat
    16141 ctgacgcaga cgtgccgcta tcacttctga aaggtagcgc tcggtcactt caactggagc
    16201 gttttctcca atcacttcga cagtgaattt ttccttatca cttgcatctt gcggataggc
    16261 aataccgtag ttgtatttca agttttcagc aatactcttt gaagttgtca atacttttga
    16321 aatatcgttt gtgatataat ctccaccttc ttggtagatg tttgtgtatt gcaactcttg
    16381 gccacgcata acagccacag tcgtttgacc gccaccaaga tcaatgacag tcgcaccaaa
    16441 ctcacgttca ccttcattca atacagagcg agttagtgcc aacggagaaa ttacaatatt
    16501 ttcaacttgg acacctgcac gctccaccgt tttacgaaga ttgtgaagaa tggtacgtgg
    16561 tcctgtgtac agcataccac gcatttccaa gcgaattccc atcattccac gagggtcctt
    16621 aatgccttgg aatccatcga cagtgaactc ttctggaata aatgaaatca cttcgcgttc
    16681 cggagtcata ctctttgtca atgctgattt tacaacattc tcaacatcca attctgtaat
    16741 ttcttgtgaa tctgttgtaa caggaatcat cccttgagtc ggttcaatct gaagaaggtt
    16801 ggcaggtaat ccaacattta ccttttcaat gcggatacct gacttttctt ctgcctgctc
    16861 aatcgctttt ttaatcgctc cagcagccac ctcaatattt acaataattc catctttgac
    16921 gccagcactt tttacattgc tgaccccgat cacattcatt tcgttatcaa tatattctgc
    16981 aaccaagact ttaacggagc ttgtaccaat atctaatccc gtaaaaaagc cgtttctagc
    17041 cattctatca gtcctctctt acttccaact ttaacaaatc atcttccatt ttgaaaaata
    17101 gtataattag tcatcattca ttataccata ttatatcgaa ttatggtgtt ttaaaggatt
    17161 tttttattac tttttcttta caaaattatc tatttcagct tgcatatcta taaataccaa
    17221 cttccatatc aattgttatt ggaacagtca cttctgctgc tatttttgta tagtaggaca
    17281 ccttctggct aatctcactc aacgggacta atacagtatt tccatctctc atattgaatg
    17341 tcagcaaatc cgcggtcact ttacttggcg ttaaatttat tgtctgaata gcagaacgaa
    17401 ttcctgcatc tattttcccc aattcaatgg ctagttcctt gattaattcc ttgtcggata
    17461 aattgattgt tgtatagtct tctggcaagg aatcctgtga aatcggttca ccgccaatct
    17521 ctccactaga caatactgga taccactgac tttgttgctg aatgtagcct attattgcgt
    17581 attctttgat gtgaatggta aaggtagctg gaaactggaa cttgattgtc gctgtctcaa
    17641 cggacgaact attcttctta atattctttg cgtaagcatc tgcatgcaaa gctatagtca
    17701 cattgtaatc atccggagag ataaggctat aattttctac ttgctcagca gttagtcgct
    17761 catttccgac aacctctatt tgttttttct tgctggttgg agaaataaaa tacaaggcta
    17821 gagctgccag taacaaactc gtcacaagaa ccggaatagc tttccagact actttggaag
    17881 gaatatcaac ttttaaagtt tgaacttttt cccctgtttc ctttttagat tccagactct
    17941 ctgtttttct ttctattccc tgaaaaagga cccttttact ctttttagct tctgggttct
    18001 cctgttctgt ctgtccctcg tcaactgcct gaatttccgc agcatcttta tgagcaaggt
    18061 aggcttgatg gcgtgctttc cattgttcga aaaaggcact tttttcatca tccggagcag
    18121 tcatctcagg tatgtctaca tccgtataaa catccgtaga aagtcctttt tcttcggcta
    18181 atacagattc tttctctgct ggtgaaattt gttcggaatc caattctact tgggatgcct
    18241 gttccacttc tagcactgat tcttctacat ttgaatcttt ttccgtcata gaacctacct
    18301 tcccatatct tctctaagca aattgtaaaa actatctact gactgaattt cattagaatt
    18361 tgtcatgttt tggatataaa attccttatg tttcaatagc tcatcgactt ctactaacag
    18421 gctggcaaat gtcaattgac tttcatcaat ctgcttagaa tatccctttt tctctgcata
    18481 gagagcattt tcaatctgat cccctcgact ggcctgtcgt cctagaggca caatcaaatg
    18541 gagttgttgc atggcaatca actcaaacag agtattggaa ccaccacggg taataacgac
    18601 atccgctaaa tccatcaacg gttgataaag ttctgtcaca tagtcaactc tatataggtt
    18661 tttatctaat tggttgaaac tactgtcccc tgtcaagttg ataatattga atcgttcggt
    18721 cagagcagca ctattctgac tgattaaatc attaaagacc ttagcaccgc ccgaaccacc
    18781 tacaaacagt agggtcggta gacttttatc aaagtgactg agaatttctg gcaactggat
    18841 tggtgtaact ttgtcattcg tttgccccac ttttgtaacc gcaccaacat gttttacttt
    18901 agtcaagctg gcaggttgct caaaagtact atacatggtt gtcgcaaatt tataggctat
    18961 tttattggcc agtcccattg ttaagtctga ttcatggata aagaccggaa cccgcagcaa
    19021 attagcagca ataactggcg gaaccgatac aaacccacct tttgaaaaaa gagcttgcgg
    19081 acgaatttta gcaataatgg ccaaggattg aagaacacca aaaccaactt taaacacgtc
    19141 caacatattt tggaaagaga aataacggcg caattttcca gtcgatatag aatgaaaatg
    19201 gacatctaaa cctgatttca caacctgctc atactcaata ccgttcccat caccaatata
    19261 gtggacttcc cagccttctt ccaaaaatcg cggaatcaaa aggagattga gcgtcacatg
    19321 cccaacagtc cctccgcctg taaaaacaat ttttttcatg attatttcaa ctcctcaaag
    19381 gtctgtaaga agacgtcgcc acgcacttca aaattggcat acatatccca gcttgcattg
    19441 gctggactta aaaggacaat atctcctttt tccgcaatgg caaaggcttt tctagtcgca
    19501 tccgcaatat cgagagcttc tatcgtttcc acttctgcct tatctgctgc acgttgaaca
    19561 cggggagctg attgaccaag aatcaccatt ttctttagtc cgaccaaatc agggactaat
    19621 tcatcaaatt cattgccacg gtccaagcca cctgcaatca aaatgacctt gctattgtca
    19681 aaccctgaaa gagctttttg agtcgccaag atattggtag acttactatc attgtagaag
    19741 gaaaccccat tgatctcacc tacaaattgc aagcggtgtt tgacacctcc aaaggcagac
    19801 aaactttcct gaatcgcttg gtggtctatt cccaacaatt ttgcaaccac aattgttgcc
    19861 aaggcatttt caacattgtg tgaacctgga acaccaattt gatcagcatc catgatgtat
    19921 tcacccttga aatagagttt tccatctgcc aaataagctc catcaactac ttccagcgta
    19981 gaaaaaggaa ctacagttgc tttggtctgt ccagccaatt ccttagtctc tttctggttg
    20041 aagttgagca ctacaaaatc ctcagccgtc atctgacgct gaatcatcca tttagctgct
    20101 acatagtctt caaaagtccc atgataatca atatgactgg caatgaggtt ggtcacaacg
    20161 gcgatatgcg gatggaaaga ttctgtcccc attaactgga aagatgataa ctccatgacc
    20221 aaggtatcct ctgcagtcgc agtttgagcg accgaagatg ctgggtaacc aatatttcca
    20281 cataattttg caggtttatt tccaacattc aagacttccg caatcatggt cgttgtcgtt
    20341 gtcttgccat tcgaacctgt aatccccacg attggagcat ctgataccaa gtaggccaat
    20401 tcaacctctg tccatactgg aattcctttt ccaattgctt tttcaaccat aggattatca
    20461 tagcgaattc ctggattttt taccatcaaa gcgaaatctt catccaataa ttccaaggga
    20521 tggctcccgc aaataacacg gataccatct tccaacaaag attgagcagt cggattttct
    20581 tcaaaaggct ttccatcgtt aacagtgacg atagcaccca atttatctag gagacgggct
    20641 gcagattccc ctgatttagc taaacctagc actaacactt tcttattttt tacaatatca
    20701 atcatcttca tctcaattac ctttactttc taacacctta ttttaccctt tacgaagacc
    20761 tttttcaagc cagaggacag aaaaccgg
//
LOCUS       DATUCG010000026        20605 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.027, whole genome shotgun sequence.
ACCESSION   DATUCG010000026 DATUCG010000000
VERSION     DATUCG010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20605
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.027"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            136..849
                     /locus_tag="VCA20_000772"
     CDS             136..849
                     /locus_tag="VCA20_000772"
                     /inference="COORDINATES: protein motif:HMM:NF012546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEP1834623.1"
                     /translation="MRIFHRKKCIFYVYGAYGKDDSVKIDPIVSSIINSDFLYAVVHV
                     RGGGFLGGDWYRSGKELNKKNSITDFVNGVKYLIENGIVDSNSLGLISSSAGAIIAGA
                     SFNDNPNLFKGILLFSPFIDPYGALLSKNDPLSKTEVAEWGDIRDIRIRKYIKDYSPM
                     QNIEKVKQSNSTIISLLGKNDKYIDNSSVVAWSDRLQMKKVNSLVYLNENAGHGGLSH
                     HDRDLFATIMSYFLSIVNS"
     gene            946..1668
                     /locus_tag="VCA20_000773"
     CDS             946..1668
                     /locus_tag="VCA20_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1834624.1"
                     /translation="MVNAIEVENLVKEYKIDNYRKNRVIDNVSFSVKYGEFTSILGIS
                     GSGKTTLLKCISGLMKPSSGQVKINGRNPYKLTNREAAKLRRKEISFIFQSYNLVPAL
                     PVIENIALPLRLSNEKVDKNAIMEILRKLECDVDIMSMVTCLSGGEKQKVAIARTLLS
                     GSHIIFADEPTGALDSESRKIVFDYLKKLSKEGRCIIMVTHDIELASKTDRALILKEG
                     QIYKDLSFPTSEQLFHALEIIK"
     gene            2311..2535
                     /locus_tag="VCA20_000774"
     CDS             2311..2535
                     /locus_tag="VCA20_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP1834625.1"
                     /translation="MYRRIRDIREDHDLTQQNVSGYLSITRSAYAKIERGEHTLSAEV
                     LIQLSNLYNVSTDYLLGQTDFPRRIKNNLK"
     gene            2821..3049
                     /locus_tag="VCA20_000775"
                     /pseudo
     CDS             2821..3049
                     /locus_tag="VCA20_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775620.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
     gene            3072..4274
                     /locus_tag="VCA20_000776"
     CDS             3072..4274
                     /locus_tag="VCA20_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEP1834626.1"
                     /translation="MATKSSKKYKGVYCDNKGKIFYQIDLGIDPVTGKRVQKKARKNQ
                     YGKPFTTMKEAYDELVRIKYEFANKVSLENYNMTFENYMNKIYLRAYKQKVQSVTYKT
                     ALPHHKLFIQYFGLKPLKAITPRDCEAFRLHIIENYSENYAKNLWSRFKACMGYAERL
                     GYISNMPCKALDNPRGKHPETPFWTYEEFQTFIKSFDLHDYEELQRFTAIWLYYMTGV
                     RVSEGLSLCWEDIDFDKKFLKVHTTLEKDENGNWYRKDQTKTPAGERLIELDDITIEV
                     LQVWRKNQFANQETDFIISRFGDPFCKSTICRIIKRKAQQVGVPVITGKGLRHSHASY
                     LINVLKKDILYVARRMGHADKSTTLNTYSHWFNALDKTVSEEITQNIKSAGLDSILCQ
                     NSCQTSNL"
     gene            4457..5206
                     /locus_tag="VCA20_000777"
     CDS             4457..5206
                     /locus_tag="VCA20_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEP1834627.1"
                     /translation="MKSYKNYIFDFYGTLVDIRTDENKLEVWNQLTQIYNAFGCSYRP
                     RQLKNAYHRFVEEAESSLSETVSYQYVEIDLETIFIRLLTEAPNENQSTNKPTDLETF
                     GQVVAAIFRVLSREKLEAYENTLTSLQTLKDREARLFILSNAQRIFTQAEIEQTGCAD
                     LMEKIYISSDFKMKKPEPAFLQLVLEENNLDVEETVMVGNDLTSDIAIAHELGMDSIL
                     LNTFPYSQEEIDSYRRLGWNFEVVGDIAELL"
     gene            5402..6736
                     /gene="trmFO"
                     /locus_tag="VCA20_000778"
     CDS             5402..6736
                     /gene="trmFO"
                     /locus_tag="VCA20_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEP1834628.1"
                     /translation="MSQTHINVIGAGLAGSEAAYQIAKRGIPVKLYEMRGVKPTPQHK
                     TDKFAELVCSNSFRGDSLTNAVGLLKEEMRRLDSIIMRAGEAHRVPAGGAMAMDREDF
                     SQAVTDEIHSHPLIEVIRGEITEIPDDAITVFATGPLTSDALAEKIHALNGGDGFYFY
                     DAAAPIVDSSTINMDLVYLKSRYDKGEAAYLNAPMNKEQFTAFYEALISAEEAPLKSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPEDYKGPRDGEYKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLEQT
                     FATKKNPNLFFAGQMTGVEGYVESAASGLVAGINAVRRFRGEEPVIFPQTTAIGALPF
                     YITHTESKHFQPMNVNFGIIKELDGPRIRDKKERYEAIVERSLKDLEEFLAY"
     gene            6814..7818
                     /locus_tag="VCA20_000779"
     CDS             6814..7818
                     /locus_tag="VCA20_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlkZ family DNA glycosylase"
                     /protein_id="HEP1834629.1"
                     /translation="MHHDTWKGIILQKQGLLKPQSVQQICQNLNGLQAQFQPYVHVGF
                     RNRMTAEDFHEGSWQEELTRQWSIRRTVHAYLKSEIPLYIHEGRLASTDYLKTEGRDG
                     FSPQTKQKYHQLILEALEQGPMTREDLKALCRGEKMTQEEEKLVFNAWGGLFRYMVER
                     GEVYQEYGAKRFHRLTDFQPLSREKAELEIARRYFAGFGPVSLADARYYFKENKSTVL
                     KWMEQLDLKTVEVAGEERFYLGELEEAELPDCLFVAGFDQLLLGYEKKANPFFNPKYI
                     RNIYTLTGIVKPVVFYKGRFVATWKRDKGTILLDIFEDITQQEEKELEHYRQMFEKIL
                     "
     gene            7932..8759
                     /locus_tag="VCA20_000780"
     CDS             7932..8759
                     /locus_tag="VCA20_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEP1834630.1"
                     /translation="MNKSYFYLDMRTHELDVPFTGRKRRVRVLLPKGYAENTEETYPV
                     VYFHDGQNVLYSKEAFSGHSWKVIPTIKRNPDIAKMIVVAIDNDGADRMHEYAAWKFS
                     EMGIPGVQFGGKGTLYAEFVMDVVKPFIDQEYRTKSDKVHTAMIGSSLGGNITQFMGL
                     AYQNQIGCLGVFSSANWLHQDAFDRYIERQQLDSEQRVYIYVGTEEADDTDKTLMAGN
                     IKQAYINSSLTYYRQLIAGGVDLDNIALEVVSGAVHNEEAWALYLPACLRFLSEHWS"
     gene            8790..9536
                     /locus_tag="VCA20_000781"
     CDS             8790..9536
                     /locus_tag="VCA20_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002909732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEP1834631.1"
                     /translation="MHVEFLSHWSGNLGREMNLNRYGHAGIPVVVFASSGGSYNEYAD
                     FGMIEACRGSIEAGQVQFFTLTSYDSESWLADWKSIHDKAEAHRAYERYVIEEAIPFI
                     KHKTGWFDGMMITGCSMGAYHALNFFLQHPDVFTKVIALSGVYDARFFNNNHDYGHDD
                     AVYQNSPSDYIWNQNDGWFIDKYRQADIIVCTGLGDWEQDGLDSFYNLKRAFEEKNIP
                     AWFDTWGTDVAHDWVWWRKQMPFFLHSIGL"
     gene            9555..10721
                     /locus_tag="VCA20_000782"
     CDS             9555..10721
                     /locus_tag="VCA20_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HEP1834632.1"
                     /translation="MNYLVISPFYPENFQPFTIELAKKEGVTVLGIGQEPYDQLPEEL
                     RNALTEYFRVNDLENIDEVKRAAAFLIYKHGPIDRVESHNEHWLENDAALREQFNIFG
                     AKPKHLKKTKFKSEMKKYFKKAGVPVVPGVVVKSERDFGRALKNVGFPMIAKPDNGVG
                     ASGTFKIENKEDFDRFVETWDGATIYFLEKFVNSSIITTYDGLVDHEGNVVFETGLTY
                     VHTPLELMLSRKDNAYFIEKELDPKLVKYGRAIIKAFGMRERFFHIEFFKDGDDYIAI
                     EYNNRMAGGFTVEAYNYAHSIDLFRDYANIVTGSDVEERRFDSQYCLVATRRDTTDYV
                     YSVDDIHQKFAEKIKTVKRMPDAFAELQGNDAYLLVTESKKELDEMIAYIGLIK"
     gene            11013..11737
                     /locus_tag="VCA20_000783"
                     /pseudo
     CDS             11013..11737
                     /locus_tag="VCA20_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905967.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-carotene 15,15'-monooxygenase"
     gene            11836..11976
                     /locus_tag="VCA20_000784"
     CDS             11836..11976
                     /locus_tag="VCA20_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834633.1"
                     /translation="MSFKFLQAKRIEEKVFSEKEKAELMEQALFLEELAHDLQSIRFM
                     AY"
     gene            11989..12378
                     /locus_tag="VCA20_000785"
     CDS             11989..12378
                     /locus_tag="VCA20_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NisI/SpaI family lantibiotic immunity
                     lipoprotein"
                     /protein_id="HEP1834634.1"
                     /translation="MKKKVFGLLALGSIILTACSSSSAGISFDEGYQTFHYRGKDYAV
                     SKQEVAEDSIQGTEVKFMEWPVVKEEGAVKKTVSLNNLYITTSKEWAIGVQDGYYKVD
                     LEDNIAESDRIDYQALLDTVDLSKENN"
     gene            12387..13139
                     /locus_tag="VCA20_000786"
     CDS             12387..13139
                     /locus_tag="VCA20_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1834635.1"
                     /translation="MLLEINHLEKVYRTRFSKEETRALQDVDFKVEEGEFIAIMGESG
                     SGKTTLLNILATLDKPTRGSVILNNQDITKIKESKLADFRLRNLGFVFQDFNLLDTLS
                     IQDNIFLPLVLARTSYEEMEKRLKVLAPKLRIQDLLKKRPFELSGGQKQRVAIARSLI
                     TQPQIVLADEPTAALDYRNSEDLLNLFEEINLDGQTILMVTHSANAASHAKRVLFIKD
                     GRIFHQLYKVGKSNQDFAKEISLNMSALLGGE"
     gene            13140..15110
                     /locus_tag="VCA20_000787"
     CDS             13140..15110
                     /locus_tag="VCA20_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP1834636.1"
                     /translation="MFYLKLAGQNIRKSIGVFAPFVLASLVLFTLICSMFLLMLSPVM
                     KTMGTGAVSIGLGVVVLTIFSLIMEIYSFNFLMQQRTREFGLYNMLGMNKKKVALVAS
                     IELFFIFLLVIVLGSALAGVFANVLYLIFVNLTNYSDLHFSISPLAFAMTAFLFAGIF
                     FILELLAIRTIGKTSPLILFRASEKGEKEPRGNLLLAALGVLSLGVGYYLSLSSKAAG
                     VGVIYRFFIAVLFVVAGTYLFYISFMTWYLKARRKNKRYFYKPDYFITTSQMIFRMKQ
                     HATGLANITLLAVMAFVTIATTTSLYTGMSSMTGALYPKETSITYPVADRSQGEGAYQ
                     QSVLSHFPEKSENSLTYLTYQAGLVYDGGSEIVISPEKISNPDFSKMAFTYFITQDDF
                     RKLGNDLPELAANQVAFMRPSEMPSLEKVTLGDSSFENVVNLDTAIFPDITNTLNAAV
                     MVVSDDSVLETIRSYYEANNPQGYQVSLDYRVFTDLTNEEVAKVGEQSGEIYEILDEN
                     GEYLGNVMQRTDFNNMLMGFTGGFLFTGFLLGISFLLGAALIIYYKQYSEGYEDKKSY
                     KILQEVGMSQKAVKKTINSQTLLVFFMPLAMATLHFVVALVMLKQMLMMFGVVSSSMI
                     YTVSGITLLAVALIYFIIYKWTSRTYYRIIER"
     gene            15201..15440
                     /locus_tag="VCA20_000788"
     CDS             15201..15440
                     /locus_tag="VCA20_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEP1834637.1"
                     /translation="MAAITLKVSEQDKLFMQAMAKFEGVSLSELIRTKTLESLEDEYD
                     ARVADIALAEYEADLAKGIEPITLEQMAEELGIEL"
     gene            15430..15687
                     /locus_tag="VCA20_000789"
     CDS             15430..15687
                     /locus_tag="VCA20_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEP1834638.1"
                     /translation="MSYKVILSAKAQKQIRKMDKTAASLILRYLYKHIDGCDNPRQYG
                     KALTANRSGQWRYRIGNYRVIVSIEDDKLIMTSIKMRNGSI"
     gene            15726..16394
                     /locus_tag="VCA20_000790"
     CDS             15726..16394
                     /locus_tag="VCA20_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002274458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEP1834639.1"
                     /translation="MMKKEKIYIVEDDEMIVQLLKQHLGKSYQVESVQNFRAVSQEVA
                     EIKPDLVLMDISLPYYNGFYWTTEIRKTMTMPIIFISSSDDEMNAVMAMNMGGDDFVS
                     KPFSLGILDAKIGAFLRRVNQFSKSTDLSIDQFQLSLDGRFTNESEQVQLSPTETKIL
                     SALLERQGQIVSKEELLEKLWENEEFIDQNTLNVNMTRLRKKVSEVGFERIHTVRGVG
                     YLVK"
     gene            16391..17332
                     /locus_tag="VCA20_000791"
     CDS             16391..17332
                     /locus_tag="VCA20_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEP1834640.1"
                     /translation="MIAKFIREYRSFYFSYIVLATSFILLFFLYRLPTEFLQNALLLS
                     LTLIILLTVGLFWKFRNRMRALEDYVHEEALPLLNKPVDLAYLNLIEAEKEATREQLL
                     DYKSREEDLQAMVKMWSHQMKVPLAALSLMSQTDNLNQQDVNHQLLRLDNYMANLLNY
                     LKLTNHASDFRFEQVEVREVAVDLVKKYRNQFLQKDISVIIDGNWLLKTDKKWLSFAL
                     SQIIDNALKYNKTGGMVAIELNGGISISDTGIGILAEDIPRLFDEGFTGFNGREHQKA
                     TGFGLYLTKRVLNQLELAISVESQLEVGTTVSITKVQ"
     gene            17408..18916
                     /locus_tag="VCA20_000792"
     CDS             17408..18916
                     /locus_tag="VCA20_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEP1834641.1"
                     /translation="MSHHHVLQNGSDIRGIAIATDEYAVNLTPQATQEVVRGLIHWLT
                     QKPELAQAYQKGQLTIGIGRDSRLSGPDLVAAFTEEAVRLGVQLIDFGMATTPALFMS
                     TQYPQFKCHAGVMITASHLPYYFNGIKIFSENGGAEHKDIDFILSHSEDLPASSLGSI
                     TSADLITPYAQDLVGKIRTACGGQEKPLTGLNIIVDAGNGAGGFFAEKVLAELGADTT
                     GSQFLDPDGTFPNHVPNPDNKEAMESIRQAVLKQGADLGIIFDTDVDRAALVTKSGEI
                     LNRNNLIAVLSQIVLAEHPGTSIVTNSPTTEHLKVFIESLGGKQIRYISGYRNVINRA
                     ILANQEGVDCQLAIETSGHAAFKENYFLDDGTYVAAKILMLLPKLQAEGKSLDDLIAQ
                     LKQPLETQEVRFKLEAADYRALGEQVIADLRNTSLEGFAFNPENEEGVRFDLTQPYGD
                     GWLLLRMSLHEPLLVLQVENDQTGYIPAVLKTLSAFLDQYPAVNQEKLKELL"
     gene            complement(18979..20049)
                     /gene="xerS"
                     /locus_tag="VCA20_000793"
     CDS             complement(18979..20049)
                     /gene="xerS"
                     /locus_tag="VCA20_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012658412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEP1834642.1"
                     /translation="MRRELLLEKIDQLKEIMPWFVLEYYQSKLAVPYSFTTLYEYLKE
                     YRRFFEWLQDSDLVSVERITDIPLDILEHLTKKDMEAFILYLRERPLLNANTTQNGVS
                     QTTINRTLSALSSLFKYLTEEVENEQGEPYFYRNVMKKVSTKKKKETLAARAENIKQK
                     LFLGDETMEFLDYVDKEYQVNLSKRSLSSFQKNKERDLAILALLLASGVRLSEAVNLD
                     LRDVNLKMMMIEVTRKGGKRDSVNVAGFAKPYLEAYMSIRQQRYKAEKTDTAFFLSEY
                     RGLPNRIDASSIEKMVAKYSADFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAN
                     TQVTDLYTHIVNDEQKNALDQL"
     gene            complement(20378..>20605)
                     /gene="tnpA"
                     /locus_tag="VCA20_000794"
     CDS             complement(20378..>20605)
                     /gene="tnpA"
                     /locus_tag="VCA20_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HEP1834643.1"
                     /translation="MLVSIPPRLSVSSFMGYLKGKSALMMFDKRANLKYKFGNRHFWA
                     EGYYVSTSTVGLNEATIKKYIQEQRNYPVNV"
BASE COUNT         6264 a         3856 c         4427 g         6058 t
ORIGIN      
        1 gtaaagaaac tcttaagttt gaattttcta aatctttatc gaaaaatagt aattacaact
       61 ataaatataa aatgatttgg acctcttgtg ataaaaaaag tgtaaagata ccgatttcac
      121 tattgtggaa gggtgatgag gatattccac agaaaaaaat gtatttttta tgtttacggt
      181 gcatacggaa aagatgattc agtaaaaatt gatccaattg tatcttcaat tattaactcg
      241 gattttcttt atgcagtagt ccatgtaaga ggtggtggct ttttaggagg cgactggtat
      301 cggtcaggaa aagaattgaa taagaaaaac tcaattacag attttgtaaa cggagtaaaa
      361 tatttaattg aaaatggaat tgttgatagc aactcattag ggctaatctc atctagtgca
      421 ggtgcaataa tagcgggagc atcgtttaat gataacccaa acctatttaa gggaattttg
      481 ttgttctctc cttttattga tccttatgga gcacttttga gtaaaaatga tccactatca
      541 aaaacagaag tagctgaatg gggggatatt cgtgacataa ggatacgaaa atatatcaag
      601 gactattctc caatgcaaaa tattgaaaaa gtgaaacaat caaattcaac aatcattagt
      661 cttctaggaa aaaatgataa gtatatagat aatagttcgg tagtggcttg gtcagataga
      721 ttgcaaatga agaaggtaaa ttcacttgtt tatcttaatg aaaatgcagg acacggagga
      781 ctttctcatc atgataggga cttatttgca acaataatga gttacttttt atcgattgta
      841 aacagttgag tttattttat ttcctgagat gtaggatatt gttaatattg tccttattta
      901 ttgtatttaa ttattttgtg aagaataatg agaggagagt gaaatatggt taatgctata
      961 gaggtagaaa acttagtaaa agaatataaa attgataatt atagaaaaaa tcgtgttata
     1021 gataatgttt ctttttcagt taaatatgga gaatttacat cgattttagg cattagtgga
     1081 tctggtaaga caacattatt gaaatgtatt tcaggtttga tgaaaccatc aagtggtcaa
     1141 gttaaaataa atggtagaaa cccttataaa ctaaccaatc gtgaggctgc aaaattacgt
     1201 aggaaagaaa tttcatttat ttttcaatct tacaatttag tcccagcttt accagtaatt
     1261 gaaaatattg cattgccgct acgtttatca aatgaaaaag ttgacaagaa tgcgataatg
     1321 gaaattttga gaaagctaga atgtgatgtt gatattatgt ctatggtgac ttgtttgtca
     1381 ggtggcgaaa aacagaaggt agcaattgca agaacacttc tgtcaggaag tcatataatt
     1441 tttgcagatg agcctactgg agccctagat tcagaatcaa gaaaaattgt atttgattat
     1501 ctaaaaaaac tatcaaaaga aggtagatgt ataataatgg taactcatga tattgaattg
     1561 gcttcgaaaa cagatcgagc tctcatacta aaagaaggtc aaatttataa agatttatcc
     1621 tttccaacat cagaacaact tttccatgct ttagaaatta ttaagtaaag cttatttttg
     1681 gaagaattaa ttatttttac ggaatctact catttcttaa tactgattga tgaataacta
     1741 agaacattaa atttcaatat tgaatcgata aaggttgatt cttctatcaa tattaagata
     1801 tttaatttct ttacagagaa aagcatatta acttgaatta gaaaaaggcc gattttaaat
     1861 tatagtgtca atagttcaca ccctaaataa ttttagagtt cccaaattga taagctatga
     1921 tatttcttgt ttctagttcg taatgctata ttttggactt gtacagtgat gtggtatttt
     1981 tagagttgaa atactttagt atatttttga ataataaggg catacttgtc cttatttttt
     2041 attgggaatt acacattcat cagttctgca attcatgcca ataataatat gcttgaaagg
     2101 tattttgctc tctaactaaa acaaattgaa ttaaagcaaa gaatattgaa atattgtttc
     2161 ctatatttct ttcacaactt tcaataattt ggatagatag tgaaaattca ttttaaattt
     2221 tagaaattgt ttgcgtacaa ttgtccaatt tgtccaattt tcaagagtat ttaaaatagt
     2281 attctatact aactcttagg agaaagctta atgtatcggc gaataagaga tatacgagag
     2341 gatcatgact taactcagca gaatgtatcc ggttatctta gtattacacg ttcagcatat
     2401 gcaaagattg agcgaggaga acatacatta tcagctgaag tgctaataca actctcgaat
     2461 ctttacaacg tcagtacaga ttatttgttg gggcagactg actttccccg ccgaataaag
     2521 aataatttaa aatagtccct cctgatttgt tcattgacaa ttgaataatt ctagatggga
     2581 ctttatttat ttgttgagca atagaatata atgcgccagg aaatgagcga aaagtcattt
     2641 attcaaaaat aggatggtaa cctatctgcc atgacacaaa taaggataat gataatgata
     2701 cttctgagcg ttcaggctgc catcgtaaaa ggacagcggg tgaaattccc atgattgaac
     2761 taaccattca aatcctatct agcgtgtaaa aaaactttgt gattttaagg aggaaagtgt
     2821 atgagaactg ctacatataa agatttaatg aacttaggat tttcctgaac atacctctag
     2881 agacattatt cgtgaagcta aaagaattgc tgtaaaaaag tttgaagaag ctcgcgaagt
     2941 tgaccataat gcggtacaat taagtaaatc gccctttgac aatcgaagac taggtatcgc
     3001 accagcagaa attgtggaac aattaatagg gattccacta tctaaataac atttagaaag
     3061 tgagaatcca aatggcaaca aaaagtagta aaaaatacaa aggtgtttat tgtgataata
     3121 agggaaagat attttatcag attgatcttg gaatcgatcc agtaactggc aaaagggtac
     3181 aaaagaaagc tagaaagaat cagtacggta agccatttac tacgatgaag gaagcgtatg
     3241 acgaattagt tcgaataaaa tatgaatttg ccaacaaagt cagtttagaa aattacaata
     3301 tgacttttga aaactatatg aataaaattt atttaagagc gtataagcaa aaagttcagt
     3361 ctgtgacata taagaccgct cttcctcatc ataaactttt tattcaatat tttggtttaa
     3421 agccattaaa agcaataact cctagagatt gtgaggcttt tagattgcat atcatagaga
     3481 attattctga aaattatgcg aaaaatttat ggtcaaggtt taaagcttgt atgggttatg
     3541 cagaaagact tggctatatt tcaaatatgc cttgtaaagc attagataat cctagaggta
     3601 agcatccaga aactccattt tggacctatg aggagtttca gacatttatc aaatcatttg
     3661 acttacatga ttatgaagaa ttgcaacgat ttactgccat ctggttatat tatatgacag
     3721 gtgtacgagt gagtgaaggt ttatctttat gttgggagga tattgatttt gataaaaaat
     3781 ttctaaaggt acatacaacc ttggagaaag atgaaaatgg aaattggtat cgtaaagacc
     3841 aaacaaagac tccagcaggt gaacgactta ttgaattgga tgatataact attgaagtac
     3901 ttcaagtttg gcgaaaaaat cagtttgcaa atcaagagac agattttatc atttcaagat
     3961 ttggagatcc tttttgtaaa tcaaccattt gtcgtatcat caaacgtaaa gcgcagcaag
     4021 taggagtgcc tgttataaca gggaaaggtc ttagacatag tcatgcttct tatctaataa
     4081 atgttttgaa aaaagatatt ttatatgtgg caagacgaat gggacatgct gataaatcaa
     4141 caactttgaa cacatatagt cattggttta atgctttaga taaaacagtt tctgaagaaa
     4201 tcacacaaaa tataaaaagt gcaggattgg attcaatttt atgccaaaat tcctgccaaa
     4261 cttcaaattt atgaactcaa accccttgat actgcacatt taacaggttg tgcaattgtt
     4321 actcttaaat aatagagttt ctaatcaaaa taaaaaccta tcacctaatg tggtaggttt
     4381 tttcgttcta ttgttgcaaa cgttttcgtt gtggggtata atataaaaaa tgctgtctaa
     4441 agattaggag aaagacatga aaagttacaa aaactatatc ttcgattttt atggtacatt
     4501 ggttgatatt cgtacagatg agaataaact agaggtctgg aatcagctaa cacagattta
     4561 caatgctttt ggttgttcct atcgaccacg ccagttgaaa aatgcctatc atcgttttgt
     4621 agaagaggca gagagctctc tctcggaaac agtgagttat cagtatgtcg aaattgattt
     4681 agaaacaatc ttcattcgtc tattaacgga agcaccaaat gaaaaccaaa gtaccaataa
     4741 gccgactgat ttagagactt ttgggcaagt agtagccgcg attttccgcg tgctttcacg
     4801 tgaaaagtta gaggcttatg aaaataccct aactagtcta cagacattaa aggatagaga
     4861 agcgcgatta tttattctct ccaatgctca acgaattttt actcaggcag aaattgaaca
     4921 gacaggttgt gctgatttga tggaaaaaat ttatatctct tctgacttta agatgaagaa
     4981 gcctgaacca gcatttcttc aactggtttt agaggaaaat aatcttgatg ttgaggaaac
     5041 ggttatggtt ggtaatgatt tgacatccga tatagctatt gctcatgaat tgggaatgga
     5101 ttccattttg ctgaatactt tcccgtatag tcaggaagaa atcgactctt ataggagact
     5161 tggttggaat tttgaggttg ttggagatat agcggagcta ttgtaaataa gcgatagaac
     5221 agtaaaggat atacataaaa tatattatgt aaaaagacaa aactactttc atttcaatga
     5281 aggtagtttt gtcttttcta gccattttct ggtatactag ataggtataa aatttagaaa
     5341 ggttctgttt tggggtattc ccaaacagta gtcatatatt ctggctagag gtattagttt
     5401 catgtctcaa acccacatta atgttatcgg agctggcttg gctggctcag aagctgctta
     5461 tcagattgcc aaacgtggca ttcccgtcaa actctacgag atgcgtggtg tcaagccgac
     5521 tccccagcac aagaccgaca agtttgccga gttggtttgt tccaattcat tccgtggtga
     5581 tagtttgacc aacgctgtcg gccttcttaa ggaagagatg cgtcgtttgg attccattat
     5641 catgcgagct ggtgaagctc accgtgtgcc tgctgggggt gccatggcca tggaccgtga
     5701 ggatttctcc caagcggtta ctgatgagat tcacagccac ccactgattg aggtcattcg
     5761 tggcgagatt acagagattc ctgacgatgc tatcacagtt ttcgcgacgg gaccattgac
     5821 atctgacgcc ttggcggaga agattcacgc gcttaatggt ggtgacggtt tttacttcta
     5881 cgatgcggca gcaccgattg tcgacagctc gaccattaac atggacttgg tttatcttaa
     5941 gtcccgttac gacaagggag aggctgccta tctcaatgca cccatgaaca aggagcagtt
     6001 caccgctttc tatgaggcct tgatttcagc cgaggaagca ccgctcaaat cctttgaaaa
     6061 agaaaaatac tttgaaggct gtatgcccat tgaagtcatg gccaaacgtg gtatcaaaac
     6121 catgctctat ggcccaatga agccagttgg tctggaatat ccagaagatt acaagggccc
     6181 acgtgatggc gaatacaaga caccttacgc agttgtccag ctccgtcagg ataacgcagc
     6241 aggtagcctt tacaatattg ttggcttcca gactcacctt aagtggggcg agcagaagcg
     6301 ggtcttccag atgattccag gactcgaaaa tgctgagttt gtccgctacg gcgttatgca
     6361 ccgcaattcc tacatggatt cgccaaactt attggaacag acctttgcga ccaagaaaaa
     6421 tccaaatctg ttctttgcag gtcaaatgac aggagtagaa ggctatgttg agtctgccgc
     6481 ttctggttta gtggccggta tcaatgctgt tcgtcgcttc cgtggcgaag agccggtcat
     6541 cttcccgcaa acaacagcta tcggagctct gccattctac atcactcaca ccgaaagcaa
     6601 gcacttccag cctatgaatg tcaactttgg tatcatcaag gagctggacg gaccgcggat
     6661 ccgtgacaag aaggagcgtt atgaggcaat agtagagcgt tccctcaagg acttggaaga
     6721 gtttttggct tactaagata tttagactgt catcagacac cttcgggtgt cttttgtgtt
     6781 atactggggt tagaaaaccg aaggagaaac acgatgcacc acgatacttg gaagggaatt
     6841 attctgcaga aacaaggtct gttgaaaccc cagtctgttc agcaaatctg ccaaaatctc
     6901 aatggtttgc aggctcagtt tcagccctat gttcatgtcg gatttcgtaa ccgtatgaca
     6961 gcggaggatt ttcatgaggg tagctggcag gaggaactga ctcgtcagtg gtccattcgg
     7021 cgaacggttc atgcctatct caagtcggaa attcctctct atatccacga ggggcggctg
     7081 gccagcaccg attacctcaa gacagaagga cgggacggtt ttagtcctca gaccaagcag
     7141 aaatatcacc agctgatttt agaagccctt gaacaaggtc ccatgacccg tgaggatctc
     7201 aaggcccttt gccgtgggga aaagatgaca caggaagagg agaagctggt ctttaatgcc
     7261 tggggcggcc tttttcgcta catggttgag cggggggaag tctatcagga gtacggtgcc
     7321 aagcgttttc atcgtctgac agactttcag ccactttctc gggaaaaagc agagctggaa
     7381 atcgctcgac ggtattttgc aggttttggg ccagtcagtt tagcggatgc tcgttattat
     7441 ttcaaggaaa ataaatctac ggtcctaaaa tggatggagc aattagattt aaaaaccgtt
     7501 gaagtagcag gagaagaacg cttttatctg ggagaattag aagaagcaga actgcctgac
     7561 tgcctcttcg ttgcaggatt tgaccaactt ttactaggct atgaaaagaa agctaatccc
     7621 ttttttaatc ctaaatacat tcgtaatatc tatactttga cgggtattgt aaaacctgtt
     7681 gtcttttata aagggcgatt tgtggcaacc tggaaacgag ataaaggtac tattttgcta
     7741 gatatttttg aagacatcac acaacaagaa gaaaaagaat tggagcatta ccgccaaatg
     7801 tttgagaaaa ttctgtgaca ttaactaact atgttcggaa aatccgaaaa ttgcaaataa
     7861 ataactaaat tttctgaata ttccagaaaa actatgatat aatacttaaa atacataagg
     7921 aagagataga tatgaataag tcatattttt atttggacat gagaactcat gaattagatg
     7981 taccatttac aggaagaaaa cgtcgagtcc gtgttttatt gccgaaagga tatgctgaaa
     8041 atacagaaga gacttatccc gttgtttatt tccacgatgg acaaaatgta ctctacagta
     8101 aggaagcttt ttcaggtcat tcatggaaag tcatcccaac tattaagcgt aatccagaca
     8161 ttgcaaaaat gatcgtagtg gctattgata acgatggtgc tgatcgcatg catgaatacg
     8221 ctgcttggaa gtttagtgag atggggattc ctggggtgca gttcggtggt aagggaacct
     8281 tgtatgctga atttgtgatg gatgtcgtca aaccatttat tgatcaagaa taccggacaa
     8341 aatctgataa agttcacact gcaatgattg gctcatcctt gggaggcaat atcacacaat
     8401 ttatgggatt ggcttatcag aaccagattg gctgtctggg agtattttca tctgccaact
     8461 ggcttcatca agatgctttc gaccgttata ttgaacgtca acaattagac tcagagcaac
     8521 gagtctacat ctacgttggg acagaagaag cagatgatac agataaaacg ttgatggctg
     8581 gcaatatcaa acaagcctat atcaattctt cattgactta ctatcgtcaa ttgatcgctg
     8641 gcggagtgga tttggacaat atcgccttgg aagtcgtttc aggtgcggtc cataatgaag
     8701 aggcttgggc gctttatttg ccagcttgcc tacgattcct aagcgaacat tggtcataaa
     8761 acaaaaatat acaaatagga ggctttacta tgcacgttga atttttaagt cattggtctg
     8821 gaaatcttgg ccgagaaatg aatctaaatc gttatggtca cgcagggatt cctgttgtcg
     8881 tatttgcttc atcaggtggt tcttacaatg aatatgctga ttttggtatg attgaagctt
     8941 gtcgtggctc tattgaagct ggtcaggtcc aattttttac tctaactagc tacgatagtg
     9001 aaagctggtt ggcagactgg aaatccattc atgataaggc agaggctcac cgcgcctatg
     9061 aacggtatgt gattgaagaa gcgattccct ttatcaaaca taaaactggc tggttcgatg
     9121 gtatgatgat aactggttgc tcaatggggg cctaccacgc gctcaatttc ttcctccagc
     9181 atccagatgt cttcacgaaa gttattgcac tttcaggtgt ctatgatgct cgattcttta
     9241 acaacaacca tgattatggt catgatgatg cggtctatca aaactcaccg tctgattaca
     9301 tctggaacca gaatgatggt tggtttattg acaaataccg tcaagcagat attatcgtct
     9361 gcacaggttt gggcgattgg gaacaagacg gtctggactc attctacaac ctaaaacgtg
     9421 cctttgaaga gaagaatatc ccagcttggt ttgatacttg gggaacagat gtggcccacg
     9481 actgggtgtg gtggagaaag caaatgccat tcttccttca ttctatcgga ctttaagatt
     9541 agaaaggact acctatgaat tatctcgtta tttcaccttt ttatccagaa aattttcaac
     9601 cgttcaccat tgaattggct aaaaaagaag gcgttacggt tctaggtatc gggcaagaac
     9661 cttacgatca attgcctgaa gaacttcgta atgccttaac agaatacttc cgtgtcaatg
     9721 atttggaaaa tatcgatgaa gtcaagcgtg cagcggcctt cttgatctac aaacatggac
     9781 caattgaccg tgtagaatcc cacaatgaac attggctcga aaacgatgca gccctccgtg
     9841 aacaattcaa catttttggt gctaagccta aacacctgaa aaagaccaaa tttaagtctg
     9901 aaatgaaaaa atatttcaaa aaagcaggtg ttccagtcgt tccaggtgtg gttgttaaat
     9961 cggaacgtga ttttggcaga gctttgaaga atgttggttt tccaatgatt gctaaaccag
    10021 acaacggtgt tggagcatct ggtaccttca aaatcgaaaa taaagaagat tttgatcggt
    10081 ttgtggagac atgggatgga gcaactattt atttcctcga aaaatttgtc aactcaagta
    10141 ttatcacaac ctatgacgga ttggtcgatc atgaagggaa tgttgttttt gaaacaggtt
    10201 taacctacgt tcatactcct ctggagctaa tgttgtctag aaaggacaat gcttacttca
    10261 tcgagaaaga actggatcca aaactagtaa aatatggtcg cgcgattatc aaggcatttg
    10321 gtatgagaga gcgttttttc catattgaat ttttcaagga tggggacgac tacattgcca
    10381 ttgaatacaa taaccgtatg gcaggcggtt tcacagttga agcctataac tacgctcatt
    10441 cgattgacct tttccgtgat tacgcaaaca ttgtaacagg tagtgatgtt gaagagagac
    10501 gattcgattc acaatactgc ttggtggcaa ctcgtcgtga tacaacagac tatgtttact
    10561 cagtagatga cattcatcag aaatttgccg aaaagattaa gacggtcaaa cgcatgccag
    10621 acgcctttgc ggaattgcaa gggaacgatg cctacctact tgtgacagaa agcaagaaag
    10681 agttggatga aatgattgcc tacattggtc taataaaata aaactaactg taagacgcca
    10741 gaaatggtgt ctttttttct ttccttacaa agctgtaagt tttttaatca gaactgtaag
    10801 agagcacagg aggaatattt tctataatag actcataaga aatagaaaga aggaaatcaa
    10861 tatgtcattc aaatttttac aagcaaaaca aatttttggt aaatcacaag aaagcaaacg
    10921 gactagaaga acaagacaaa caagctgaat ttctattcga gctagaacat gaactgttcc
    10981 aacgaaactg gatgtactag gaggtttgac tggtgaaatc atataatgct tatcaactga
    11041 aactggccat ggcaggtttg atggttttgg atcatctgaa aatcatacct aattttatct
    11101 ctgatcaact cgctttggtg tttcatttag taacacggtg cgtcgcagtc ttctttgcct
    11161 acatgttggt cgaaggctat cttcatacga gaaatgtcaa agcttatttg ttacgactgt
    11221 atggtgcagc aggattcatg gctcttggaa atactttgct aaacagcctt taccagtcca
    11281 aagagattta cattagcaat aatatctttt tgaccttagc agtcggctta accctgctgg
    11341 cttgtcttag aaattttagt gggcacacga gagtgaagca aatcattcag gtcctgctgg
    11401 caggattatt gacaatcata ggagtcttgt ttacagaagg ggggcacggt ggtctgccat
    11461 tcatcctcat cacctatttg ggtctactaa gcgaaccatt gcctatggtt ttctggctct
    11521 agttttatta gccatgtcct accagcccta cgaaaggctt gagatgacca ttcagatgat
    11581 gctctacaat gcagactggc tatttatttt agtccttcca attctcagtc tttataatgg
    11641 acaagcaggg ccccgcactg cattttcacg ctatttcttt tacattttct atccgctaca
    11701 cttgtggcta ctggcaacca ttgcctactt tatctaacct tacaatcttg taagtttttt
    11761 aatcagaact gtaagagagc acaggaggaa tattttctat aatagactca taagaaatag
    11821 aaagaaggaa atcatatgtc attcaaattt ctacaagcaa agagaataga agaaaaggta
    11881 ttctcagaaa aagagaaagc tgaactcatg gaacaagcct tattcttaga agagctcgcc
    11941 catgatttgc aaagtatacg atttatggca tactagaggg aggaagctat gaaaaagaaa
    12001 gtatttggct tgctggcttt gggcagcata atcctaaccg cttgttcttc ctcttcagca
    12061 ggcattagct ttgatgaggg ctatcaaacc tttcattata ggggaaaaga ctatgccgtt
    12121 agcaaacagg aagttgctga agatagtatt caaggaacag aagttaagtt tatggaatgg
    12181 cctgttgtta aagaagaagg ggcagtgaaa aagacagtat ccttgaataa tctctatata
    12241 accactagca aggaatgggc catcggtgtc caggatggtt attataaggt ggatttagaa
    12301 gataatattg ctgaatcaga ccgaatcgac tatcaagcat tacttgatac agttgattta
    12361 tccaaagaaa acaattagga gtttgtatgt tattagaaat caatcattta gaaaaagttt
    12421 atcgcacgcg cttttcaaaa gaggaaactc gtgctttgca agatgtggat tttaaggtag
    12481 aagagggcga gtttatcgcc atcatgggtg aaagtggttc aggtaagacg accttgctta
    12541 acattctagc aaccctggac aagccgacga ggggctctgt tatcctcaat aatcaagata
    12601 ttaccaaaat caaggaaagc aagctggcag atttccgttt gcgtaaccta ggttttgttt
    12661 tccaagattt taatctcttg gataccctgt ccatccaaga caatatcttt ttacctctgg
    12721 tattggcacg tactagctat gaggaaatgg aaaaacggtt gaaagttttg gcgccaaaac
    12781 tgcggattca agatttgctg aaaaaacgtc cttttgaact ttctggtgga caaaagcagc
    12841 gcgtggccat tgctcgtagt ttgattaccc agccacagat tgtcctagca gacgaaccga
    12901 cagcagccct agattaccgc aattcggaag acttgctgaa tttgtttgag gaaatcaacc
    12961 tagatggaca aacaattctc atggtaactc actcagccaa cgccgccagc catgccaaac
    13021 gcgtcctctt catcaaggat ggtcggattt tccaccaatt gtacaaggta ggtaagagca
    13081 atcaagactt tgccaaggag atttcgctca atatgtctgc acttttaggg ggtgagtaga
    13141 tgttttacct caaacttgct ggacaaaata tccgaaaatc cataggtgtt tttgctccct
    13201 tcgttttagc tagtctggtc ctctttacct tgatttgttc tatgttcttg ctcatgctta
    13261 gtccagtcat gaaaaccatg ggaacaggtg ccgtgtctat cgggctgggt gtcgttgtat
    13321 tgactatttt ctcgctgatt atggaaatat atagctttaa tttcctcatg caacaacgga
    13381 ctagggagtt cgggctttac aatatgctgg ggatgaacaa gaagaaggtg gccttggttg
    13441 ccagcattga gttgtttttt atcttcctct tggtcattgt gctgggctct gcattagctg
    13501 gtgtatttgc taatgttctc tacttaattt ttgtgaatct gaccaattat agtgacttac
    13561 actttagtat ttcacctctg gcatttgcca tgaccgcctt tctctttgca ggtatcttct
    13621 ttatcttgga attactggct attcggacta ttggcaagac cagtccgctg attttattcc
    13681 gagctagtga aaaaggggag aaggagccac gtgggaatct cctcttggct gctctcggtg
    13741 tccttagcct aggtgttgga tactacctat cactttcatc taaagctgct ggtgttggtg
    13801 tcatttatcg tttctttatc gcagtgctct ttgtcgttgc ggggacctat ctgttctaca
    13861 tcagttttat gacttggtat ttgaaggctc gtcggaaaaa caaacgctat ttttacaagc
    13921 cagattattt cattacgact tcgcagatga ttttccggat gaagcagcac gcaacaggtt
    13981 tggccaatat tacgctactt gcggtcatgg cctttgtgac cattgcgact acaacgtctc
    14041 tttatacagg tatgagcagt atgacaggtg cactatatcc aaaggaaaca tctattacct
    14101 atcctgttgc ggatagaagt cagggtgaag gcgcctatca gcaatcggtg ctcagccatt
    14161 ttccagaaaa atcggaaaat agtctgacct atctgacata ccaagctggt ctggtctatg
    14221 atggtggtag tgagattgtc attagtccgg aaaagatttc taatccagat tttagtaaga
    14281 tggcttttac ctactttatc actcaagatg atttcaggaa gttgggcaat gacttgccag
    14341 agctggctgc caatcaagtt gcctttatgc gtccgagtga aatgccgtcg ctagaaaaag
    14401 tcactttggg agatagtagc tttgagaatg tagtaaatct tgatacagct attttccctg
    14461 atattactaa tacgctcaat gctgccgtca tggtggttag cgatgatagt gttctggaaa
    14521 ccattcgtag ttactatgaa gcaaataatc cgcaaggtta tcaggttagt ctggactacc
    14581 gtgtctttac cgacctgacc aatgaagaag tagcgaaagt cggtgagcaa tctggtgaaa
    14641 tatatgaaat tctcgatgaa aatggggaat atcttggaaa tgttatgcaa cgtacggatt
    14701 tcaacaatat gctcatgggc tttacagggg gtttcctctt tacaggcttc ttgttgggca
    14761 ttagtttctt gctaggtgca gccttgatta tctactacaa gcagtattcg gaagggtatg
    14821 aggacaagaa atcctacaag attcttcaag aagtggggat gagccagaag gctgtcaaga
    14881 aaacaattaa ctcccaaacg cttttggtct tctttatgcc attggctatg gcaaccttgc
    14941 attttgtcgt cgcccttgtc atgctcaagc aaatgctgat gatgttcggt gtggtttctt
    15001 ccagcatgat ttacacggtt agtggtatta cattacttgc agttgccttg atctacttta
    15061 tcatctacaa gtggaccagc cgaacctatt atcgcattat tgaacggtag cagaagtctc
    15121 gcttatgcgg gatttcttga tttttttagg aaatggtgtt acaatggtaa taccaaagga
    15181 ataccagaag aggtgagaaa atggcagcta ttacattgaa agtttctgag caggacaagc
    15241 tctttatgca ggctatggca aagtttgaag gtgtcagttt gtcagaactc attcggacta
    15301 aaacccttga atcccttgaa gacgagtacg atgcgcgtgt ggctgatatc gctctggctg
    15361 aatacgaggc agacttggcg aaaggcattg agccgataac attagaacaa atggctgagg
    15421 agttgggcat tgagttataa ggttattttg tctgctaagg ctcaaaagca aattcgaaag
    15481 atggataaaa ctgctgcaag tttgattttg cggtatcttt acaagcatat tgacggttgc
    15541 gacaacccta gacaatacgg aaaagccttg actgctaatc gttctggcca atggcgctat
    15601 cgtatcggca actacagggt tatcgtcagt attgaagatg acaaattgat tatgacaagc
    15661 ataaaaatga gaaatggaag tatatagaaa gccaagacct gtgtcctggc ttttacagaa
    15721 tgaagatgat gaaaaaagaa aagatttata ttgttgaaga cgatgagatg attgtccaac
    15781 tcttgaagca gcatttggga aaatcgtatc aggtggaaag tgtgcagaat ttccgagctg
    15841 tcagtcagga agttgctgaa atcaaaccag acttggtgct gatggacatt agcctgcctt
    15901 attacaacgg cttttactgg acgacagaaa tccgtaaaac catgaccatg cccattatct
    15961 ttatctcttc tagcgatgat gagatgaatg cagtcatggc tatgaacatg ggaggcgatg
    16021 attttgtttc caagcctttt tcactaggga ttttggatgc caaaatcgga gccttcctgc
    16081 gtcgggttaa tcaattcagc aaatcaactg atttgtcaat tgaccagttt caacttagtt
    16141 tagacggacg atttacaaac gaatcggaac aggttcaatt gtctcctacg gaaaccaaga
    16201 ttttgtccgc cttactagaa cgacaagggc agattgttag caaggaagaa ctcttggaaa
    16261 aactctggga aaatgaagaa tttattgacc agaacacgct caatgtcaac atgaccagac
    16321 tgcgtaagaa ggtgagcgaa gtcgggtttg aacgaattca tacggttcgt ggcgtggggt
    16381 acttggtcaa atgatagcaa aatttatccg tgaataccgg agtttttact tctcctacat
    16441 tgttctggct actagtttta tcttgctttt ctttctctat cgactgccca ccgaattcct
    16501 gcaaaacgct ctgctattgt ccctaaccct tatcatccta ctgactgtag ggcttttctg
    16561 gaagtttcgc aaccgcatga gagccttgga agactatgtc catgaagaag ctctgcccct
    16621 gcttaataag cctgtggacc ttgcctatct caacttgatt gaagcggaga aggaggcgac
    16681 gcgggagcag ctcctggatt ataagtcacg agaagaggac ctgcaagcca tggtcaaaat
    16741 gtggtcccat cagatgaaag tgcctctggc tgccctgtct ttgatgagcc agactgacaa
    16801 tctcaaccag caggatgtca atcaccagct cttacgcttg gacaattata tggcaaatct
    16861 cctcaactac ctcaagctga ccaaccatgc cagcgatttt cgctttgagc aggtggaagt
    16921 gagagaagtg gcggtcgatt tggtcaaaaa ataccgcaat cagttcttgc aaaaggacat
    16981 ttcagtgatc atcgatggaa attggctact gaagaccgat aagaaatggc tcagctttgc
    17041 cctatcacag ataattgaca acgccctcaa gtacaataag actggtggta tggtagccat
    17101 tgaattgaat ggtggaattt ccatttccga tacgggaatt gggattctgg cagaagacat
    17161 tccacgcctg tttgacgaag gttttacagg ttttaatggc cgtgaacacc aaaaagctac
    17221 aggatttggc ttgtatctga ccaagcgggt cttgaatcag ttggaactag ctatttccgt
    17281 ggaaagtcaa ctggaagttg ggacaactgt aagcatcaca aaagtacagt agaagctgtg
    17341 cttttttcat tttgaaaccg ctttgaaaat atgatatact aaatttagtt tgaattagga
    17401 ggttactatg tcccatcatc acgtcttgca aaatggatca gatattcgtg gaattgctat
    17461 cgctacagac gaatatgctg tcaatctcac tccgcaagcc acccaggaag tggttcgcgg
    17521 acttattcat tggctaactc agaagccaga actggcgcag gcctatcaaa aaggccaatt
    17581 gaccatcggt atcggtcgag atagccgcct cagcggtcca gatttggtgg cagcctttac
    17641 agaagaagcc gttcgtctgg gcgttcagct gattgacttt ggcatggcca ccacgccagc
    17701 cctcttcatg agcacccagt atcctcagtt caagtgccac gctggtgtca tgatcactgc
    17761 cagccacctg ccctattact tcaacggcat taagattttt tcggaaaatg gcggagcaga
    17821 acataaagat attgacttca tcctctcgca tagtgaagac ttgccagctt cttcccttgg
    17881 aagtatcacc agtgcagacc tgattacgcc ttatgcccaa gacttggtgg gtaaaattcg
    17941 taccgcctgt ggtggacaag aaaaaccact gacaggtctt aatatcatcg tcgatgctgg
    18001 aaacggtgca ggcggatttt ttgctgagaa agtgttggct gaactgggag cagatacgac
    18061 aggctcacaa ttcctagacc ccgacggaac cttcccaaat cacgttccaa accctgataa
    18121 caaggaagct atggaaagca ttcgtcaggc agtcttgaag caaggtgcag atcttggtat
    18181 tatctttgat accgacgttg accgtgccgc cctggtcacc aagtcaggag aaattctcaa
    18241 tcgaaacaac ctgattgccg ttctcagcca gattgtccta gctgaacatc caggaaccag
    18301 cattgtgacc aattccccaa cgactgaaca cctaaaagtc tttattgaaa gcctcggtgg
    18361 caaacaaatc cgctatattt caggttaccg taatgtcatt aaccgtgcta ttttagctaa
    18421 tcaagaagga gttgattgcc agttggctat cgaaacctca ggacacgctg cctttaagga
    18481 aaattacttc ctcgatgacg gaacgtatgt ggcggctaag attcttatgc tcttacccaa
    18541 actgcaagcg gaaggcaagt ccttggacga cttgattgcc cagcttaaac aaccgctgga
    18601 aacccaagaa gtgcggttca aactagaagc ggcagattat cgagcactgg gtgaacaggt
    18661 cattgctgat ctcagaaaca ctagcctcga aggctttgct tttaacccag aaaacgaaga
    18721 aggagtgcgg tttgacctga ctcagcctta tggtgacggc tggctcttgc tccgtatgag
    18781 ccttcacgag ccactcttag tcttgcaggt agaaaacgac caaaccggct atattcctgc
    18841 cgttttgaaa accttgtcag cctttttgga ccagtaccca gctgtcaatc aggaaaaatt
    18901 gaaagaattg ctataatcga aaacacgagt aagtttgagc ttactcgtgt tgttttacat
    18961 aattcatatt acgtaacatt acaactgatc cagtgcattt ttttgctcat cgttgacgat
    19021 atgagtatag aggtcggtga cctgggtatt ggcgtgtccc agctgatgac tgaccagaac
    19081 ttgcgacttg gtggcgtcgt agagccgtgt tgccagcgtg tgacggagtt tgtggggtgt
    19141 cacacgtacc ttgaagtccg cagagtactt ggcaaccatt ttttcaatag aagaagcgtc
    19201 gatacgattg ggcagaccac ggtattcgga caggaagaag gctgtatccg ttttttcagc
    19261 cttgtagcgt tgttgacgga tgctcatata ggcttccaga tagggcttag caaaccctgc
    19321 cacatttacc gagtctcgct tgccaccttt acgcgttacc tcaatcatca tcatcttgag
    19381 gtttacatct cgaagatcca aattcactgc ctctgataga cggacaccag atgccaagag
    19441 aagagccaga attgccaagt cccgctcctt gtttttctgg aaggaggaga gggaacgttt
    19501 agagagattg acctggtatt ccttatccac atagtccaaa aactccatgg tttcatcgcc
    19561 caaaaagagc ttttgcttga tgttctccgc ccgagccgct aaggtttcct tctttttctt
    19621 ggtggacacc ttcttcatga cattgcggta gaagtagggc tcgccctgct cattttccac
    19681 ttcttcggtt aaatacttga agagactaga aagagccgat agagtacggt taatggtggt
    19741 ctgcgataca ccattctgcg tggtattggc gttcagtaag ggacgctccc gcagataaag
    19801 aatgaaagct tccatatctt tttttgtcaa atgttccaga atatccaagg gaatgtcagt
    19861 aatccgttcc acagatacca aatccgaatc ctgcaaccac tcaaaaaaac gacgatattc
    19921 tttcaagtat tcatacaagg ttgtaaaact gtaaggcaca gccaattttg actgataata
    19981 ttccagaaca aaccaaggca taatttcttt tagttgatca attttttcta ataataactc
    20041 gcgtctcatc attttctcct gaattttgct atatgagtag tatagcataa ccttatataa
    20101 gtttcaagaa aattatagtt tttcggaaag atgtttgtag ggatatcaga gggataacca
    20161 aaaaaacata ataactggaa tttttttcac acccaaaatc ccaaaaatct agattatttc
    20221 tcgttaaact cacttagcat tagaatttcc tccgcaacct cttctggctt caaactgtct
    20281 gtgtttaacc tctgccccgg aatgtgactt tcaaaagctt ttaaacaaac atcaagatgt
    20341 ctcttggccc agatgttgtc ttcattgact cgattatcta aacgttcact ggataatttc
    20401 tttgttcttg tatatatttc ttaattgtgg cttcatttag tccaaccgta cttgtactta
    20461 cataatatcc ctctgcccag aaatgacgat tgccaaattt atatttgaga ttggcgcgtt
    20521 tatcaaacat cataagagca ctcttacctt ttaaatatcc cataaagctt gatacactta
    20581 atcgcggagg aatactgact aacat
//
LOCUS       DATUCG010000027        20579 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.028, whole genome shotgun sequence.
ACCESSION   DATUCG010000027 DATUCG010000000
VERSION     DATUCG010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20579
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.028"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            <1..91
                     /locus_tag="VCA20_000795"
     CDS             <1..91
                     /locus_tag="VCA20_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEP1834644.1"
                     /translation="WAHIKKHLRRVLPNCDTFLEVLSSCSCFS"
     gene            complement(92..2014)
                     /locus_tag="VCA20_000796"
     CDS             complement(92..2014)
                     /locus_tag="VCA20_000796"
                     /inference="COORDINATES: protein motif:HMM:NF016724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEP1834645.1"
                     /translation="MKLSELKILLNKVNLNIKLLENFENVELTRFHIKKTELYNYQKE
                     KEFKEWQTKLPKLILEELINCTHTQDEKQLKNNFFPSNIFENRDEWQTILIISESLEQ
                     YISEYRMNSFLNLLTLQENLQYVIKKEKYPFLKFIQSQTTRNKLNTAYQEIELHSKEV
                     VKLEENLSNWKKLINSDSSSNQLEYFYHHQKEYFTQLINQLGILSDSNSDYILETLHL
                     SLNKKLRHIKKHSKMGLSLAINNTWDEIKEKDFNRQSQSYPIELLENTDIQIERYFPN
                     LSKHFKFLMNIKNSSNEHISKLCAIPLEDVLSIKKVVANILQSSKLNFFPKLSVDTFS
                     EHEYKLINLLTIYQAYPIEKEKLEQKIVLELESLIAALKQLEATTPNRLQLNFQTDER
                     RDSWLNAESELYKRYAAILREYSFEDRVEDILDKDKLIELFIENNATYYALIEETTGS
                     KKKYIPNDLPSIIVEKVKQNEINRLGLKVVLRSYQAFGVQYVLHYKNVLLGDEMGLGK
                     TIQAIAIANHLFHNKKIYTVVVCPLSVLENWNREIQKWSTLPTFVYRGNLRQTLLHQW
                     QQNGGVLLTNYEQVKTLLTSIRNKNIDFLIIDEAHYIKNPNTMRAKYSMELSELADYK
                     LFMSGTPLENRFSEML"
     gene            2153..3187
                     /locus_tag="VCA20_000797"
     CDS             2153..3187
                     /locus_tag="VCA20_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEP1834646.1"
                     /translation="MKTLDYIVTLTNTPSPTGFTTDIMNYIKAEVESFGYAASKTAKG
                     GVLVTVPGENDQEHRMVTAHLDTLGAMVRAVKPDGRLKMDLVGGFGYPSIEGENCLIH
                     CAKNGKTFTGTILMHQTSVHVYRDASTAERNQTNMEIRLDEKVTNADETRALGIEVGD
                     FISFDPRTVVTETGFIKSRHLDDKVSAAILLHLLKVYKEAGATLPYTTHFYFSNNEEI
                     GYGANSSIPAQVVEYLAVDMGAMGDDQQTDEYTVSICVKDGSGPYHYELRQHLVALAE
                     RDGIPYKLDIYPYYGSDASAAMRAGADVKHALLGAGIESSHSYERTHLDSVVATERMV
                     DAYLKSAMVD"
     gene            3189..3926
                     /locus_tag="VCA20_000798"
     CDS             3189..3926
                     /locus_tag="VCA20_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834647.1"
                     /translation="MNETRFLELFNDLDKVLRKVCGVADGEYADVGSMLTKAKELTRH
                     NPVEVNWDKLYVARQLRNLMVHEKRTGLKEVAQPSQELITVLEKVIAQYQEPITIADY
                     LQKTQKIKPVIFEGKDKLIKALEIVEQEHFSKFPIFSDKVYQGLVSDKGLTNWLAKAS
                     KETGSIREKLVEASLEDVLACEEDSIQVIVLPITAHLYQVINHFEGRKRTTILVSCQA
                     GGKLHSPEDLVGIVTAQDLAEIYGMVD"
     gene            3945..4412
                     /locus_tag="VCA20_000799"
     CDS             3945..4412
                     /locus_tag="VCA20_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HEP1834648.1"
                     /translation="MSTNRLAWDEYFAAQALLIANRATCKRAKVGAVLVKDNKVIATG
                     YNGSVSGTEHCLDQECLMIDGHCARTLHAEVNAILQGAERGIPKGFTAYVTHFPCLNC
                     SKQLLQVGCQRVVYINEYRMDDYAQYLYKEKGCELVHLPLETVKQAIADAEFM"
     gene            4810..6000
                     /locus_tag="VCA20_000800"
     CDS             4810..6000
                     /locus_tag="VCA20_000800"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEP1834649.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAEDPDAHVAAE
                     TAVYTGSVHVFGEISTTAYVDINRVVRDTIAEIGYTKGEYGFSAESVGVHPSLVEQSP
                     DIAQGVNEALETRQDASQDPLDLIGAGDQGLMFGFAVDETPELMPLPISLSHKLVRRL
                     AELRKSGEIAYLRPDAKSQVTVEYDEDNQPVRVDTVVISTQHDPEVSQEQIRQDVIER
                     VIKEIIPAHYLDDQTNYFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLAKKAEVQLAYAIGVAHPVSVRIDTFGTSTVA
                     ESKLEAAVRQIFDLRPAGIIQMLDLKRPIYKQTAAYGHMGRTDIDLPWEKLDKVEALK
                     IAVL"
     gene            6031..6642
                     /locus_tag="VCA20_000801"
     CDS             6031..6642
                     /locus_tag="VCA20_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEP1834650.1"
                     /translation="MKALIVFAHPRKESFTHALVDRVAEALLENGAEVVIRDLYEIGF
                     DPVLRGEDTIPIEDGKFVRQATVFPADVQVEMDLIAESDLLVYIFPSWWNGMPAIMKG
                     YVDRVFQHGFAYSFESDEPCKLFSTKKAIFFTPTGQPQNADGSLTPIDQAMKSLTSEW
                     LFNSNGAQVIDHIFYGRVPYLTREELELYLVDAREQIQRIFHK"
     gene            6793..8052
                     /locus_tag="VCA20_000802"
     CDS             6793..8052
                     /locus_tag="VCA20_000802"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEP1834651.1"
                     /translation="MRKIVINGGTTLKGSVTVSGAKNSVVALIPAIILADGIVTLDGV
                     PAISDVDSLVDIMKTMGATVKRDGETLEIDPRGVKDMPMPFGKINSLRASYYFYGSLL
                     GRFGQAVVGLPGGCDLGPRPIDLHLKAFAAMGAETTYEGDYMRLATNGQRIQGAHIFM
                     DVVSVGATVNTILAAVKAEGRTVIENAAREPEIIDVATLLNNMGARIRGAGTDIITIE
                     GVDSLKGTCHQVIPDRIEAGTYIALAAAVGEGIRIDNVLYEHLESYIAKLEEMGVRMT
                     ISEDSIFVEKQEKLKAVSVKTSPYPGFATDLQQPITPLLLKAEGTGTIVDTIYEKRVG
                     HVQELANLGAKISTRNNHIAFEGPNQLTGTSVKATDLRAGAAMVIAGLMAQGRTEITN
                     IEFILRGYSNIIDKLTELGADIQLIED"
     gene            8148..8720
                     /locus_tag="VCA20_000803"
     CDS             8148..8720
                     /locus_tag="VCA20_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEP1834652.1"
                     /translation="MTLWTRLAKFAYFETDRLYLRPFSFEDGESFYEIVSNPENLPFI
                     FPALEDRNIAFSTMVEKFMRSPLGNWVLVDKYSERMIGALCFEKVDERQLSAELSYFL
                     KKDYWRRGLMTEAVRTLVYLAFYEFGLRELVIVTHEENVASQMVAKKAGLVQVAQYRG
                     SDRYSHKTRNYLKFSLKRAEFSLEMYEENQ"
     gene            8717..9577
                     /locus_tag="VCA20_000804"
     CDS             8717..9577
                     /locus_tag="VCA20_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HEP1834653.1"
                     /translation="MITIKSQREIDAMNRAGDVLAGIHIGLREIIKPGVDMWEVEEYV
                     RKTCKEKNVLPLQIGVDGELMDYPYATCCGLNDEVAHAFPRHYKLKEGDLLKVDMVLS
                     EPLDKAVVDVSKLNFNDVKAMKKITQTYRGGCADSCWAYAVGQVSEEVQNLMDVTKEC
                     LYRGIEQAVVGNRIGDIGAAIQEYAEGLGYGVVRDLVGHGVGPTFHEEPMVPHYGTKG
                     RGLRLREGMVLTIEPMINTGTWEIDTDMKTGWAHKTLDGGLSCQYEHQFVITKDGPVI
                     LTSQGEEGTY"
     gene            9625..10554
                     /locus_tag="VCA20_000805"
     CDS             9625..10554
                     /locus_tag="VCA20_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEP1834654.1"
                     /translation="MKKTKIVEGLKTFCSTFFSFYRSADSDVTSVAVAYYLLISIFPI
                     LLTLANLLPYYPFDVDMILSVVAEFVPDKLYPSVSSFITSVLTKPSSSWLGISILTTL
                     WTLSRSMTILQKAFNKAYGINEHRDFIFGHLIGIFLGIGLQVIILLSITLLTFGQAVF
                     SYINKLVPIEDAWLKGLLSQTQLVGLMALFAALVMLYFFLPNVRIKKVRYVFPGTLFV
                     LLTMTSIGKLFSIYVDNYANKLLDFRIVTAVVFLVFMLWFIFMAQVLIIGAMINATVQ
                     SMQVEEFHARDGDIVSILNRLKARFTAIDKEWE"
     gene            complement(10714..11151)
                     /locus_tag="VCA20_000806"
     CDS             complement(10714..11151)
                     /locus_tag="VCA20_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEP1834655.1"
                     /translation="MKKLFYQLINNEVILYLIAGVAATLVYMVTRMGLFLVIPSILLV
                     TLLANAVAILFAFWTNDRFVFKQAREGWPQRLVKFVSARIATLVMDMALAYLLVQAYP
                     QLIGQFVNHDLTLINAIATLFSQVLVIVLNYVLSKLFVFKDKK"
     regulatory      11180..11281
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            11279..11794
                     /locus_tag="VCA20_000807"
     CDS             11279..11794
                     /locus_tag="VCA20_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEP1834656.1"
                     /translation="MNTSKWTARDMAEIALVAAIYVALTLTPPLNAISFGAVQFRLSE
                     MLNFLAFYNRKYIIAVTIGCMISNLIGFGVIDLFVGGFSTLIFVTLGVILFEKYKKDY
                     LFGGLFNKAFFYFSFFFAATMFTIALELKVLYDLPFFLTWLTTAAGELLSLLVGAVVI
                     DKLSKKINFEK"
     gene            11910..12752
                     /locus_tag="VCA20_000808"
     CDS             11910..12752
                     /locus_tag="VCA20_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEP1834657.1"
                     /translation="MTRYTFSNGVTVPKIGFGTWQIPDGEVAYGAVSYALEAGYRHID
                     TAQIYGNEASVGRAIADSGLAREDIFLTTKVWNDKIGYEDTLASVEESMQKLQVDYLD
                     LLLIHWPNPKAIRDSIGWKKRNAQVWKALEELYRAGKVKVIGVSNFMEHHLEALLETA
                     EIIPMANQIMLAPGTPQSELVAYCKVKGILLEAYSPFGTGTLFQSQEAADLAKEAGCS
                     VAQLALAWSLDKGFLPLPKSTSPENIKANLEIDGLAISPTAVAKLDKLEGVKGRLDPD
                     QAEF"
     gene            12834..14990
                     /locus_tag="VCA20_000809"
     CDS             12834..14990
                     /locus_tag="VCA20_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEP1834658.1"
                     /translation="MEEKYLKIAQELGVSLKQIDTVLSLTAEGNTIPFIARYRKDVTG
                     NLDEVVIKSIIDRDKALTTLADRKATVLAKIEEQGKLTDQLRQAIEEAEKLADVEELY
                     LPYKEKRRTKATVAREAGLFPLARLILQNVADLEEQAASFICEGFDTAQACLAGAVDI
                     LVEAISEDNKLRAWVYHEVQTNSSLTSELKDQEADEKEVFQIYYDFSEKVAKMQGYKT
                     LAINRGEKLGVLKVTFEHNVDKMVRFFELRFPQSNSYIKDVIQQAIKKKILPAMERRI
                     RTELTEEAEEGAIQLFSKNLRNLLLVSPLKGKVVLGFDPAFRTGAKLAVVDQTGKLLT
                     TQVIYPVEPAGQRQIAQAKKDLADLIGQYQVEIIAIGNGTASRESEAFVADLLKDFPD
                     VSYVIVNESGASVYSASELARYEFPDLPVEKRSAISIARRLQDPLAELVKIDPKSIGV
                     GQYQHDVNQKSLSESLDFVVDTVVNQVGVNVNTASPALLAHVAGLNKTISENIVKYRE
                     ENGALTSRQQLKKVPRLGDKAFEQAAGFLRIPDATNFLDNTGVHPESYKAVENLLELL
                     AIDHLDEAAQEKLKQVAIVDTAEKIGVGQETLKDIIADLLKPGRDLRDDFEAPVLRQD
                     VLDVKDLVVGQELQGTVRNIVDFGAFVDIGVHEDGLVHISRMVKRKRDKNGRQQALPH
                     PSEVLAVGEIVTVWVVEVDIKRNRIGLSLLKPNGSE"
     gene            14977..15411
                     /locus_tag="VCA20_000810"
     CDS             14977..15411
                     /locus_tag="VCA20_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEP1834659.1"
                     /translation="MDLNKYVQEVSLQDFGKEFRHVAIWNRRLRSTGGRFFPRDGHLD
                     FNPKHLEEQGLEVFRKIVRHELCHYHLYFEKKGYRHGDRDFKELLAAVDGLRYAPKLE
                     QAAKPSLLYTCQSCGQVYQRKRRIDLKKYRCGKCRGLLEKIL"
     gene            complement(15595..16413)
                     /locus_tag="VCA20_000811"
     CDS             complement(15595..16413)
                     /locus_tag="VCA20_000811"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834660.1"
                     /translation="MTAKKENHSTSNTNTQISKVKPLPLQKLDNINFYQLNSRSLKSL
                     QQSQIPYSQDNHLTLAIDKWQVSFNQYQKAINQDKGKELTKKIQEANQLRNSDFSIFK
                     RSLAAFETSKRQTEKSAYTELTQIVKPFRDIHRLDYEKESLEMDKLFNRLSSPEAIEH
                     IEILGLKRFFENLKESQQEFETVFSHTTDIRSKANRYDTYQIRKDLQSAYLRVLKLCE
                     ILTELHGHSLYSELVTLFNTSRQYYIEQLSHHSNKVAKNYKNIILQLPEPLLQP"
     gene            16904..19471
                     /locus_tag="VCA20_000812"
     CDS             16904..19471
                     /locus_tag="VCA20_000812"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014570425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEP1834661.1"
                     /translation="MITSEEIKLRLWNGANELRGSMDASRYKDYMLGLMFYKFLSDQT
                     LKTYANLSGVSKESDWYQEYILTHQEYGTELEKMIQDNLGYFVRPEHLYQTWVKDMNE
                     GRFEVQKVTDSLSQFERSIAVANGSDDFQGLFASSTLDLTDTALGSNLNERSKNIQAL
                     IRLFEDLDMVALQNGDVLGDAYEYLIGQFAMESGKKAGEFYTPHQVSEVMAQIVATNS
                     SISSIYDPTVGSGSLLLTVKKHLSEDRQRSLNYYGQEKNTATYNLTRMNLLLHGVRPE
                     KMSIKNGDTLSQDWPEDPERPNEGVQFDAVVMNPPYSVKNWNRSGLKPSDPRFEIAGV
                     LPPDSKGDFAFLLHGLYHLGTHGTMAIVLPHGVLFRGAAEGEIRKRLLEKNQIDAVIG
                     LPSNLFTNTGIPVCIIILKKNRDLNEPVLFIDASRNFIKAGKQNALQEKDIAKIVDVY
                     TNRTEEEGYSHLASRQELIQNDYNMNIPRYVTALDEEIPHDVDAHLYGGIPKKNIQSL
                     RVLNQTVKEVLDNAFSENRPGYLTLNQTLEEFSRVVLSSPVVRAEYDQVQSTIAAFIE
                     EYWTKLHRLQTETNTRLLKEAMLADIKKRLSQFDQIDIYEGYQIIAEIWTKTLTHDAE
                     LIEQLGFYEAGRTREPNMVAKGKNKEKVQDGWNGVIIPNSLIASECYGDELAYIESLK
                     NRISEIDSEVSELVENAKVEDSEEYNALFDVLKKNEDGEALDSFEGKLVKAALKEAVK
                     GSTEFGFLKQVDDLAKEKSTVAKEIKTKEQELKELVSNRIPVLTDEEVDQLLYKKWFG
                     QVTDKALELVELPLKAEIAIIEELHNRYAETLDDMDAEIALLERELENLMKELVVLS"
     gene            19596..>20579
                     /locus_tag="VCA20_000813"
     CDS             19596..>20579
                     /locus_tag="VCA20_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005714764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEP1834662.1"
                     /translation="MPNKERNIPKRRFKEFENADAWELRKIFEIADRFDNLRVPIASS
                     LRVSGSTPYYGANGIQDYVEGYTHEGEFILVAEDGANDLKNYPVNLVNGKIWVNNHAH
                     VLQGKKSIADNKFLAFSINQTNIESLLVGGGRAKLNSDVLMNIELKLPSPAEQEAIGT
                     FFSTLDQHITLHQRKLDKLKSVKQAYLSEMFPAEGERVPKRRFPGFTDAWELRKLGEV
                     GTVAMCRRIFKEQTSEHGEIPFYKIGTFGGTPDAFIARELFEEFKEKYSYPNIGDILI
                     SASGSIGRTVEFLGEDEYFQDSNIVWLNHDGKINNIFLKQFYSIVKWSGIEG"
BASE COUNT         5869 a         3627 c         4752 g         6331 t
ORIGIN      
        1 atgggctcat atcaaaaaac acctcagaag agtattgccg aattgcgata cgtttcttga
       61 ggtactttcg tcctgctctt gtttcagttg actataacat ctcagagaat cgattttcta
      121 gaggtgtacc cgacataaaa agtttataat cagcgagttc acttagttcc attgagtatt
      181 ttgctcgcat tgtatttggg tttttaatat agtgtgcttc atcgattatt aaaaaatcaa
      241 tatttttgtt tcttattgaa gtcagtaagg ttttgacctg ttcataatta gtaagtaata
      301 ctcccccgtt ttgttgccat tgatgtagta atgtttgtcg caaatttcct ctatacacaa
      361 aagttggaag agttgaccat ttttgaattt ctcgattcca attttctaat acacttaatg
      421 gacaaaccac aacagtgtat atttttttat tgtgaaacaa atggtttgcg atagcaatag
      481 cttgaatagt tttaccaaga cccatctcat caccaagtag aacattttta tagtgaagaa
      541 catattgaac tccaaatgct tgatatgaac gcaaaacaac ttttaaacca agtcgattaa
      601 tttcattttg ctttactttt tctacaatga tacttggtag atcgttagga atatatttct
      661 ttttagaacc ggttgtctct tctatgagag catagtaagt agcattgttt tcaataaata
      721 actctattaa tttatctttg tcaaggatat cctcaactcg atcttcaaat gaatattctc
      781 ttaaaatagc cgcatatcgt ttgtaaagtt ctgattctgc atttagccaa ctatctcttc
      841 tttcgtctgt ttgaaagttt aattgcaatc tattaggagt agttgcttct aattgtttta
      901 aggctgcaat cagagattct aattctaaca ctattttttg ttctagtttt tctttttcta
      961 ttggatatgc ctgatatatt gtcaataaat taataagctt gtattcatgt tcggagaacg
     1021 tgtctacaga gagtttaggg aaaaaattta gcttactgct ctgtaaaata tttgccacaa
     1081 ccttcttaat ggataaaaca tcctccaaag gaatggcaca aagtttacta atatgttcat
     1141 ttgaagaatt tttgatattc attagaaact tgaaatgttt tgataggtta ggaaaatatc
     1201 tctcaatctg aatgtcggta ttttctaata attcaatggg ataactttga gattgtctat
     1261 taaaatcttt ttctttaatt tcatcccagg tattattaat agccaagcta agtcccattt
     1321 ttgaatgttt cttgatgtga cgtagttttt tattgagtga tagatgtaaa gtttcgagaa
     1381 tataatctga attggaatca cttagtattc caagctgatt gatgagttgt gtaaagtatt
     1441 ccttctgatg atggtaaaag tattcaagct gattggagga actgtcacta ttaattagtt
     1501 ttttccagtt tgaaagattt tcttctaatt tcacaacttc ttttgagtgc aactcaatct
     1561 cttgataagc tgtatttaac ttgtttctag tagtttgact ttgaataaat tttaggaacg
     1621 gatatttttc tttctttata acatattgta agttttcttg taatgttagt aaatttaaaa
     1681 aagaattcat tctatattca gatatatatt gttctagtga ttcagaaatg attaaaatgg
     1741 tttgccactc atctctattt tcaaaaatgt ttgagggaaa aaagttgttt tttaattgct
     1801 tttcatcttg agtatgagtg caatttatca attcttcaag aataagtttt ggtagttttg
     1861 tttgccattc tttaaactct ttttcttttt gatagttata taactcggtt tttttaatat
     1921 gaaagcgagt caactctaca ttctcaaaat tttcgagtaa cttgatgttt agattaacct
     1981 tattcagtaa aatttttaat tcacttagct tcatcaattt ttcttctttc cttccttcct
     2041 ttattttgat aatattttaa cacattttta tttatataaa taagtaaaat acatttcttg
     2101 atagtcattt tatttaaaac tgctataata gtcttatgaa taggaggttt tcatgaagac
     2161 acttgattac attgttacat tgaccaacac tccgtcgcca actggtttta cgacggatat
     2221 tatgaattac atcaaggcag aggttgaaag tttcggctat gctgctagta agaccgctaa
     2281 aggtggtgtt ttggtgacag ttccggggga aaatgaccag gaacaccgca tggtgactgc
     2341 tcacctggac acactggggg ccatggttcg tgcagtgaaa ccagatggcc gacttaaaat
     2401 ggatttggtg ggaggatttg gatacccttc tattgaggga gaaaattgtt tgattcattg
     2461 cgctaaaaat ggcaagactt tcacaggtac tattctcatg caccagacct ctgtccatgt
     2521 ctatcgggat gccagtaccg ctgagcgcaa tcagaccaac atggaaattc gtttggatga
     2581 aaaagtgacc aatgcggacg agacgcgggc cttgggtatt gaagtcggag attttatctc
     2641 ctttgatcct cgtaccgtcg tgacagaaac tggctttatc aagagtcgtc acttggatga
     2701 taaggtatcc gcagctatct tgcttcatct attgaaggtc tacaaggaag cgggagcgac
     2761 cttgccttat acgacacatt tctatttttc aaataatgaa gagattggct acggtgccaa
     2821 ctccagcatt cctgctcagg tggttgaata tttggcagtc gatatggggg caatgggaga
     2881 tgaccagcaa actgacgaat acacagtttc tatctgtgtc aaggacggat caggtcccta
     2941 ccattatgaa ctccgtcagc acttggtagc cttggctgag agggatggta ttccttacaa
     3001 gctggatatt tatccttact atggttccga tgcttcggca gctatgcgag caggtgcgga
     3061 tgtcaagcat gcactgcttg gtgctggcat tgagtccagc cattcctacg agcggaccca
     3121 tttggattct gtagttgcga ctgagcggat ggtagatgcc tatttgaaat cagcaatggt
     3181 agattaagat gaatgaaacc cgttttttag agttattcaa tgacttggat aaggtcttgc
     3241 gtaaggtgtg cggtgttgcg gatggggaat atgcagatgt aggctctatg ttgaccaaag
     3301 caaaggaatt gacgcgacat aatcccgttg aggtgaactg ggataaactc tatgtggctc
     3361 gtcagttgcg taatctcatg gttcatgaga aacgaacagg cttgaaggaa gtggcccaac
     3421 cttcgcagga attgatcact gttttggaaa aagtgatagc ccagtaccaa gaaccgatta
     3481 ccattgccga ctatttgcaa aagacccaga aaattaagcc ggttattttt gagggcaagg
     3541 acaaattgat aaaggcttta gaaattgtgg agcaagagca tttttctaag tttcctattt
     3601 tttctgacaa ggtttatcaa ggcctggttt ctgacaaggg attgacaaat tggttggcca
     3661 aggcaagtaa ggaaacgggc tctatacgtg aaaaattggt ggaggctagt cttgaagacg
     3721 tactagcttg tgaggaagat agtattcagg tgattgttct accaataaca gcccatcttt
     3781 atcaggtaat taatcatttt gaggggcgta agcgcacaac catcctagtc agttgtcagg
     3841 cgggcgggaa gcttcattcg ccagaggatc ttgttggtat tgtcacagcc caggacttgg
     3901 cagagattta tgggatggtg gattgagtgg agaaaggaga aatcatgtca acaaatcgtt
     3961 tagcttggga tgagtatttt gcggctcagg cgcttttgat tgccaatcgg gcgacctgta
     4021 agagagcaaa ggtcggtgcg gttttggtta aggacaataa ggttatcgca acaggctaca
     4081 atggttctgt ttcaggaacc gaacattgtt tggatcagga atgtctcatg attgacggtc
     4141 attgtgctag gaccttgcat gcggaagtta atgccatttt acaaggagca gagcgaggaa
     4201 ttccaaaagg ctttactgcc tatgtgaccc attttccctg cctcaattgc tccaagcaat
     4261 tgctacaggt tggttgccaa cgtgtcgtct atatcaatga ataccgaatg gacgactatg
     4321 cccagtatct ttataaggaa aaaggatgcg agttggttca tttaccgcta gaaactgtaa
     4381 aacaagcgat tgcggatgca gaatttatgt aatcagcaaa gaagttatca gaaagtggta
     4441 acttcttttt tattttttgt aaatgaactg ccaagatagc tgaaactatg ctatactaga
     4501 aacgttatta agtcccgaaa aggtagttta tagactagtt aatatttgca gaaacacttg
     4561 aaacacaatt aaagaagctg gtaatattga atagtaagcg taaaaacttt actacacttc
     4621 agtcaccatt ttcttcaaat aaggctgatt tcatacagtc tggtattgtc tttctcgttg
     4681 ggtatctgga aattttcctt tgcttaagac aggtgtcttt cgcaaagaac tagtcttcga
     4741 ctagttcttt gtaaccttag taacccgcta ggtttctagc atatattcta ttttaagggg
     4801 gacattttta tgtcagaacg taagttgttt acgtctgaat ctgtttcgga gggtcatccg
     4861 gataagattg cagaccagat ttcagatgcc attttggacg ctattttagc agaggatcca
     4921 gatgcgcacg tagcggcaga gacggctgtt tatacaggta gtgttcatgt gtttggagag
     4981 atttcaacca ctgcctatgt ggatattaac cgtgtggtac gggataccat tgcggagatt
     5041 ggctatacaa aaggtgagta tggtttttca gctgagtcgg ttggggttca tccgtcactt
     5101 gtagagcaat cgccagatat tgcccaaggc gtcaatgaag ccttggaaac tcgtcaagat
     5161 gctagtcaag atccattgga tttgattggt gcaggtgacc aggggctcat gtttggtttt
     5221 gcagtagatg aaacgcctga acttatgccg ttgccaattt cacttagtca caaattggtg
     5281 cgtcgtttgg ccgagttgag aaaatctggt gaaattgctt atctccgtcc ggatgctaag
     5341 tcacaggtaa cagttgaata cgatgaagac aatcaacctg ttcgtgtgga tacagtcgtt
     5401 atttcgaccc agcatgatcc agaagttagc caggagcaaa ttcgtcagga tgtcattgaa
     5461 cgtgtgatca aggaaatcat tccagctcat tatctggatg accaaaccaa ctactttatc
     5521 aacccaactg gtcgctttgt cattggcgga cctcaagggg actctggttt gacaggccgt
     5581 aagattatcg ttgataccta tggtggttat tctcgtcatg gcggcggtgc cttctctggt
     5641 aaggatgcga ccaaagtgga ccgttctgca tcttatgcgg cacgctacat cgcaaaaaat
     5701 atcgtcgcag ctggtttggc taaaaaggcg gaagttcaat tggcttacgc tatcggtgtt
     5761 gctcacccag tttcagttcg catcgatacc tttggtacga gcacagtagc ggaaagcaaa
     5821 ctagaagcag ctgttcgtca gattttcgat ttgcgcccag ctggtattat tcaaatgttg
     5881 gacctcaaac gtccgattta caagcaaact gccgcctatg gacacatggg acgtaccgat
     5941 attgatctcc catgggagaa attggataag gtggaagcct tgaaaattgc ggttttgtaa
     6001 tgaaatagtt gtccatttag tgagaagttt atgaaggcgt taattgtctt tgcacaccct
     6061 agaaaagaaa gctttaccca tgctttggtt gatagagtgg cggaagctct gctggaaaat
     6121 ggagcagaag ttgtcatccg agatttatac gaaataggat tcgatcccgt gttacgtgga
     6181 gaagatacca ttcctattga agatggcaaa tttgtacgtc aggcaactgt ttttccagca
     6241 gatgttcaag ttgaaatgga tttgattgcc gaaagcgact tactagtcta tatatttccg
     6301 agttggtgga atggaatgcc tgctattatg aaagggtacg tagaccgtgt atttcaacat
     6361 ggctttgctt acagttttga atctgatgaa ccgtgtaaat tattttccac aaagaaagct
     6421 attttcttta cgccaacagg gcagccacaa aatgcagatg gaagtctgac accgattgat
     6481 caggcgatga aaagcctgac ctcagaatgg ctcttcaata gcaacggtgc tcaagtcatt
     6541 gaccatatct tctatgggcg agtaccttat ttaacgagag aagagctgga gttgtatctc
     6601 gtggatgcaa gggagcaaat tcagagaatt tttcataaat gatgaagaga gattcactaa
     6661 tagtatataa atatgaagga ctgagctcat tggctcggtc cttttgcctg ctatataact
     6721 agctttttcc aagactttat gatagaatga aacgtatgcc tttttaggca aattaagaaa
     6781 gtgaatgtta gtatgagaaa aattgtaatt aatggtggaa caacgctgaa agggtcggtt
     6841 accgttagtg gtgcaaaaaa ctcagtcgtt gctcttattc cggccattat tcttgcagat
     6901 gggattgtta ctctggacgg agtacctgca atctcggatg tagatagttt agttgatatt
     6961 atgaaaacaa tgggcgcgac ggtcaagcgt gatggtgaga cgcttgagat tgaccctcgt
     7021 ggtgtaaaag acatgcctat gccgtttggc aaaattaata gcttgcgtgc atcctactat
     7081 ttttacggat ccctccttgg tcgctttggt caagctgtcg ttggtttgcc aggtggttgt
     7141 gatttgggac ctcgtccgat tgatttgcac ttgaaagcat tcgcggcaat gggagctgag
     7201 acaacttatg agggcgacta catgcgcttg gcaactaatg gtcagcgtat tcaaggagcg
     7261 catattttca tggatgttgt cagcgttggt gcgacggtta atactatttt agcagctgtc
     7321 aaagcagaag gccgtacagt aattgaaaat gcggcgcgtg aacctgaaat tattgatgtg
     7381 gcaacgttgc ttaacaatat gggggcacgt atccgtggtg cgggtacaga tattatcacc
     7441 attgaaggcg tagacagtct aaaaggaaca tgtcatcaag tgattccaga ccgtatcgaa
     7501 gcagggactt atattgccct tgctgctgcc gttggtgaag gtattcgtat cgataatgtc
     7561 ctttatgaac acttagagag ttacatcgct aagttggaag aaatgggtgt tcgcatgacc
     7621 atttctgaag atagtatttt tgttgaaaag caagagaaat taaaggcggt aagtgtgaaa
     7681 acttcacctt atccaggttt tgcgacagac ttgcagcaac cgattacacc tttactgctt
     7741 aaggcagaag ggactggtac gattgttgat acgatttatg aaaaacgtgt tggtcatgtg
     7801 caggagcttg caaatcttgg cgcaaaaatc tctactcgta acaaccatat tgcctttgaa
     7861 ggtccaaatc aactgactgg tacatctgtt aaagctactg atttgcgtgc gggagccgca
     7921 atggttattg ctggtttgat ggctcagggg cgtacagaaa ttacaaacat tgagtttatt
     7981 ctacgtggtt actcaaatat tattgataag ctgacagaat tgggtgcaga tattcagttg
     8041 attgaggact agagagtcta attgagaact actttctcaa aacgtaatcg atttaaaaaa
     8101 cgaaacaatc tgaggctggg agtaattctc agccccttgg gtacactatg acactttgga
     8161 caagactagc gaagtttgct tactttgaaa cggaccgttt gtatttacga ccattttctt
     8221 ttgaagatgg agaaagtttc tatgaaattg tatcaaatcc cgaaaatctt ccttttatct
     8281 ttccagcttt ggaggacaga aatattgctt tttcgacaat ggttgaaaaa tttatgcgtt
     8341 caccattggg aaactgggtc ttagttgata agtattcgga gaggatgatt ggtgcgcttt
     8401 gctttgaaaa ggtagatgaa cgtcaacttt ctgcggagct cagctatttc ctaaaaaaag
     8461 actactggag aagaggctta atgacggaag cagtgaggac tttagtctat ctagcttttt
     8521 atgagtttgg tctgagggag ctggttatcg tcactcatga ggaaaatgtg gccagtcaga
     8581 tggtggcgaa aaaagctggt ttggtacagg ttgcgcagta tcgcggaagt gaccgctata
     8641 gccataaaac aagaaattat ctaaaattta gcttgaagag ggctgaattt agtttagaaa
     8701 tgtatgagga gaatcaatga ttaccattaa atcacaacga gaaattgatg caatgaaccg
     8761 tgctggagat gttctggctg gtattcatat tggtttgcga gagattatca agccaggtgt
     8821 ggacatgtgg gaagtagagg aatatgtccg taaaacgtgt aaggaaaaga atgttttgcc
     8881 actccaaatt ggtgttgatg gagaattgat ggattatcct tatgcgactt gttgtggatt
     8941 gaatgacgag gtcgctcatg cctttccacg tcattacaaa cttaaagaag gtgatttact
     9001 gaaggttgat atggttttga gcgagccttt ggataaagcg gtagttgatg tttctaagct
     9061 caactttaat gatgtcaagg ctatgaagaa aatcacccag acctatcgtg gtggttgtgc
     9121 ggattcctgc tgggcttatg cggttggtca ggtctctgag gaggttcaaa acttgatgga
     9181 tgtgactaag gaatgtttgt atcgcggtat cgagcaagca gttgttggca atcgtatcgg
     9241 tgatattggt gcagccattc aagaatatgc agaaggtctt ggttacggtg ttgttcgtga
     9301 cttggtgggt cacggcgttg gaccaacttt ccacgaagag ccgatggttc ctcattatgg
     9361 aactaaaggt cgaggtcttc gcttgcgtga aggtatggtt ttgactatcg agcctatgat
     9421 taatacaggg acttgggaaa ttgatacgga tatgaagact ggttgggctc acaaaacact
     9481 tgatggtggt ttgtcttgtc aatacgaaca ccaatttgtc atcaccaaag atggaccggt
     9541 tattttgacc agtcaaggtg aagaaggtac ctattaattc tcggtgtggt cgtgaataat
     9601 agatgaatga ggaaggagga gagaatgaag aaaaccaaaa tagttgaagg tttgaaaaca
     9661 ttctgctcaa ccttcttttc tttttatcgt tctgctgata gcgatgtaac aagtgttgca
     9721 gtagcttact atttgttaat ttctattttc cctattcttc taactcttgc gaatttactg
     9781 ccatattatc catttgatgt ggatatgatt ttatcggttg tagctgaatt tgtgccagac
     9841 aagctctatc cttcggtttc atcttttatt acttctgtat taacaaagcc gtcgtcatca
     9901 tggttaggga tttcaattct gacaactctt tggaccctgt cacgtagtat gactattttg
     9961 cagaaggcct ttaataaggc ttatggcatc aatgaacacc gtgattttat ttttgggcac
    10021 ttgattggca tatttttagg aattggtctg caggtaatta tcctcttaag tattacgctt
    10081 ttgactttcg ggcaggcagt tttttcttat attaataagc tggttcctat tgaagacgcc
    10141 tggctgaagg gacttcttag tcaaacacag ttggtaggtt taatggctct gtttgctgcc
    10201 ttggttatgc tttatttctt tttgccaaat gttcgaataa aaaaagttcg atatgttttt
    10261 ccaggaacgt tgtttgttct gttgacgatg acttctattg gaaaattatt ttcgatttat
    10321 gttgataatt atgctaataa gttgctggat tttagaatag taacagctgt cgtctttctt
    10381 gtttttatgc tttggttcat ttttatggct caagtactca tcattggtgc aatgattaat
    10441 gctacggttc aaagtatgca ggtagaagag tttcatgcta gagatgggga tattgtttca
    10501 atcttgaata ggctaaaggc aagatttaca gcgatagata aagaatggga gtgagaaaga
    10561 actctatttt caaaaatgag ttcgtcttct cacccccgca cagttgatta ggtcagattt
    10621 ggagtgtgaa acgcgaacaa atctgccaat caaccactgc gctgagatgt tgacacgaac
    10681 tctgagaagc gaggttggtc tttttgccca acctcatttc ttgtctttaa atacaaataa
    10741 tttacttaaa acgtagttta gaacaatgac caagacttgt gagaagagtg tggcgatggc
    10801 gttgataagt gtcaaatcat ggttgacaaa ctgcccaatc aattgtgggt aggcttgtac
    10861 taataaatag gctagagcca tatccatgac caaggtcgcg atgcgggcag atacaaactt
    10921 gaccaatcgc tgaggccaac cctctctggc ttgtttgaaa acgaaacggt cgttggtcca
    10981 aaaggcaaag aggatagcta ctgcattggc taaaagagta accaatagaa tggaaggaat
    11041 gactaaaaat agccccattc gagtgaccat atagacaagg gttgctgcca cacctgcaat
    11101 caaatagaga ataacttcgt tattgatgag ttgataaaat agttttttca taggactagt
    11161 ctatcatttt tagaaaaaat atgctatact aatcaacgtt gttgaatttt caatgaccct
    11221 tggggcttgc tcactttacc caagcatatt ttaagctttt tcgctaaagg agaattttat
    11281 gaatacatct aaatggactg ccagagatat ggcagaaatt gctcttgttg cggcgattta
    11341 tgtcgctctt accttgacac cgccattgaa tgctatttcc ttcggggctg ttcagtttcg
    11401 tttgtctgaa atgctcaatt tcttggcttt ttataatcgt aagtatatta tcgctgtgac
    11461 cattggctgc atgatttcca acttgattgg ttttggtgtg attgacctct ttgttggtgg
    11521 gttttcgaca ttaatcttcg ttacgttggg agttatcctg tttgaaaaat acaagaagga
    11581 ctatctattc ggtggattat ttaacaaggc tttcttttat ttctcattct tttttgcggc
    11641 aactatgttt actattgctt tagaattaaa agtcttatat gatttaccat tctttctaac
    11701 ttggttgaca acagcagcag gagaattgct ttctctatta gttggagctg ttgttatcga
    11761 taaactatcc aagaaaataa attttgaaaa gtaagtatac aataaatgtg agcagacact
    11821 ttcgggtgtc ttttattttt aggtcgcatt attttgtatt gattgacaat ttagtttata
    11881 ctggggatag aagaaatagg aggtctttta tgacaagata tacgttttca aatggtgtaa
    11941 ctgttcctaa aattggtttt ggaacttggc agatacccga tggtgaagtg gcgtacggtg
    12001 cggtttctta tgcattagaa gctggttatc gccatattga tactgctcaa atttatggca
    12061 atgaagctag tgttggtcga gctatagctg atagtggtct agctcgtgag gacatttttt
    12121 taacaactaa ggtttggaat gataagattg gctatgaaga tacccttgct tcagtagaag
    12181 aatccatgca aaaacttcag gtggattatc tggatttgtt actgatccat tggccaaatc
    12241 ccaaggctat tcgcgacagc attggctgga aaaaacgaaa tgcccaggtt tggaaggcat
    12301 tagaagagct ctatcgtgct ggaaaagtga aagtaattgg agtttctaac tttatggaac
    12361 accatctaga agctttgctg gaaacagctg agattatccc gatggctaat cagattatgt
    12421 tagctccagg aactcctcag tcagaattag ttgcttactg caaagtaaaa gggattctct
    12481 tggaggccta tagcccattt ggtacaggta ctcttttcca aagccaagaa gcagctgatt
    12541 tggctaaaga agctggttgt agtgttgctc agctggcact agcttggtct ttagataaag
    12601 gctttctccc tctcccgaaa tcaactagtc cagaaaatat taaggcgaat ttggaaattg
    12661 atggcttggc tattagtccg acagcagttg caaagttgga taaactagaa ggagtaaaag
    12721 gtcgacttga cccagatcag gctgaatttt aattagctat tgagagcatg tagtttctct
    12781 acgtgctttt tctattgagt catttgtacc gctttcatgc tataatagta gctatggaag
    12841 aaaaatattt gaaaattgct caggaattgg gcgttagttt aaaacagatt gatacggttc
    12901 tctctttgac ggctgaaggc aatactattc cctttatcgc tcgttatcgg aaggatgtga
    12961 cggggaactt ggacgaggtg gtcatcaagt ctatcattga tcgggataag gccttgacta
    13021 ctctcgctga ccgtaaggct actgtccttg ccaagattga agaacaaggc aaattgacag
    13081 accagctccg tcaggctatc gaggaagcgg aaaagttggc agatgtggaa gaactctacc
    13141 tgccttataa ggaaaaacgt cggaccaagg caacggtggc gcgtgaggct ggactgttcc
    13201 cgctggctag gttgattttg caaaatgtgg ctgacttgga agaacaggct gctagtttta
    13261 tctgtgaagg ttttgatact gctcaggctt gtttggctgg ggctgtggat attttggtgg
    13321 aagctatctc agaagacaat aagcttcggg cctgggtcta ccatgaagta caaaccaatt
    13381 caagcctgac ttcagagcta aaagatcaag aagcagatga aaaagaagtc tttcagattt
    13441 attatgattt ctctgagaaa gtggcaaaaa tgcagggtta taagactttg gccattaacc
    13501 gtggtgagaa attaggcgtt ctaaaggtta cttttgaaca caatgtggat aaaatggttc
    13561 gtttctttga gctacgtttt ccacagtcta atagctacat caaggatgtg atccagcagg
    13621 ctatcaagaa gaaaattttg cctgctatgg agcgtcgtat tcgaacagaa ttgacggaag
    13681 aagctgaaga aggagctatc cagctcttct ctaaaaacct acgaaatctg ctccttgtat
    13741 ctcccctcaa aggcaaggtt gtccttggtt ttgaccctgc ctttcggaca ggtgctaagt
    13801 tggcggttgt agaccagacg ggaaaactct tgacaaccca ggtcatctat ccagttgagc
    13861 cggctggtca gcgccaaatt gctcaggcca agaaagactt ggcagacctg attggacaat
    13921 accaagtgga aatcattgct atcggaaatg gaacggccag ccgtgaatct gaagccttcg
    13981 tcgctgattt actcaaggac ttcccagatg tttcttacgt catcgtcaat gaaagcggag
    14041 catctgtcta ttcagcctct gaactggccc gatacgagtt tccagacctc cctgtggaaa
    14101 aacgctctgc catttccatc gcccgccgcc tgcaagaccc tctggctgag ctggtcaaaa
    14161 tcgatcccaa gtccattggt gtcggccagt accagcacga tgtcaaccag aaatctctgt
    14221 ctgaaagcct ggactttgtg gttgatacgg tggtcaacca ggtcggtgtc aatgtcaata
    14281 cggccagtcc tgcccttttg gctcacgttg ctggtctcaa caagactatt tcggagaata
    14341 tcgtcaagta ccgcgaggaa aatggtgctc tgacctctcg tcagcaactt aaaaaggtgc
    14401 ctcgtctggg tgacaaggcc tttgagcagg cggctggttt cttgcggatt cctgatgcga
    14461 cgaacttttt ggacaatact ggcgtgcacc ctgagtctta caaggctgtg gaaaacctgt
    14521 tagaacttct agcgattgac cacttagatg aggctgcgca ggaaaaatta aagcaggtgg
    14581 ctatcgttga tacggcagaa aaaatcggtg ttggtcagga gaccttgaag gacatcatcg
    14641 cagacttgct caagccaggt cgtgatttac gggatgactt tgaagcacca gtcctccgtc
    14701 aagatgtctt ggatgttaag gacctggtag tcggacagga attgcaaggt acggtccgca
    14761 atattgtgga ctttggagcc tttgtggata tcggtgtcca cgaagatggc ttggttcaca
    14821 tttcacgcat ggtcaaacgc aaacgggata agaatggacg ccaacaagcc ttgccacatc
    14881 caagcgaagt cctcgccgtt ggggaaatcg taaccgtctg ggtggttgaa gtggacatca
    14941 aacgaaatcg tatcggtctt agccttttga aaccaaatgg atctgaataa gtatgtgcaa
    15001 gaagtctcct tgcaagactt cggtaaggaa tttcggcatg tagcaatctg gaacagacga
    15061 ttgaggtcaa caggtggtcg cttttttcca agggatgggc atttggattt caatcccaag
    15121 cacttggagg agcaaggttt agaagtcttt cggaagattg tccgccacga gctctgccac
    15181 taccatctct actttgagaa gaaaggctat cggcacggag acagggattt caaggaactg
    15241 ctggctgcgg tggacggcct gcgctatgct cccaaactgg aacaagctgc caagccaagc
    15301 ttgctgtata cctgtcagtc ctgtggacaa gtctaccagc gcaagcgccg cattgaccta
    15361 aaaaaatacc gatgtgggaa gtgtaggggg cttttagaga aaatacttta atacaagaaa
    15421 accagtccta agagattaac ttagggctgg tttttgtgat aatactcttc gaaaatcaaa
    15481 atcagacgtt gttgacttga tttgatgaac ttcagttcta tctgcatctg cgtcgcctag
    15541 tctgattttg attttcattg agtataaggt attgggatta ttttgtaaaa aggattaggg
    15601 ttgaaggagc ggttctggga gttgtaaaat gatattttta taatttttgg caactttatt
    15661 tgaatggtgt gaaagttgtt cgatgtagta ttgtctacta gtattgaaaa gtgtaacgag
    15721 ttcggagtag agagagtggc catggagttc cgtcaggatt tcgcagagtt ttaaaacacg
    15781 gagataagca gactggagat ctttacggat ttgataggta tcatagcgat tagctttact
    15841 acggatgtca gtggtatgtg agaaaactgt ttcaaattcc tgctgacttt cttttaggtt
    15901 ctcaaaaaat ctttttaaac caaggatttc gatatgttca attgcttctg gactggataa
    15961 acgattaaat agtttgtcca tttcaaggct ttctttttca taatcaagtc tatgtatgtc
    16021 tctgaaaggt tttacaatct gtgttaattc ggtataagcg gatttttctg tttgacgttt
    16081 gctcgtttcg aaggcggcta gactacgttt gaaaatagaa aagtcgctat tacgaagttg
    16141 gttggcttct tgaatctttt ttgttaattc tttgccctta tcttgattga tagctttttg
    16201 gtattggttg aaagacactt gccacttatc tattgcaaga gtcaaatggt tgtcctgact
    16261 gtaaggtatt tgactttgtt gtaatgattt tagactgcga gaattaagtt ggtagaagtt
    16321 gatattgtcc aacttttgca gtggtaatgg tttcacttta cttatttgtg tgtttgtgtt
    16381 tgatgtagag tgattttcct tttttgctgt catcagctat cctcatttct tatgtgatgg
    16441 tactatgata gcaattatcc tatcttttca acaggacatt tttgtcctga aaatgaacat
    16501 ggaagtaatt aagtgccgtt tttactgcca aataattaga aatgaacgta taggtcggta
    16561 tatttctcct atgcaggtcc ccatcctact gattggttgt tggttttaat ccctgtcgta
    16621 ttaaaattat gaagataggt agtatttttc ttattttatt gtaatcacaa gataataagt
    16681 ctaaagaagt tttgcttgtg agctttcagt cgatggatta gtcgactgat ttttagtttg
    16741 ttagctgatt gaaagatttt ttgaagcatg aaaaatgaca agcaaagtca gtatattatt
    16801 caagataaat aaagaatttt attttgtatc gtggtataat tttatagaaa gctcttacgc
    16861 tattactagg cgtaagtgag gaaaacaaat acgaggagaa atcatgatta cttctgaaga
    16921 gattaaatta cgtctatgga atggtgccaa tgaactccgt ggctctatgg atgctagtcg
    16981 gtacaaagat tatatgcttg ggctcatgtt ctacaagttt ttaagtgacc agaccttaaa
    17041 aacttatgct aatttgtctg gagtatccaa agagtcagat tggtatcaag agtacatcct
    17101 tacccaccaa gaatacggta cggaattgga aaagatgatc caggataatt tgggttattt
    17161 tgtccgtcct gagcacctct atcaaacctg ggtcaaggat atgaatgaag gcaggtttga
    17221 agtccaaaaa gtaacggata gcttgagcca gtttgagcgg agcatcgctg ttgctaatgg
    17281 ttctgatgat tttcaaggtc tatttgccag ctcaaccctt gacttgacag atacagcact
    17341 gggcagcaat ctcaatgaac gcagtaaaaa tattcaagcc ctcatccgtt tgttcgagga
    17401 tttggacatg gttgccctgc aaaatggaga tgtcttggga gatgcttatg aatacctaat
    17461 tggtcaattt gccatggaat ctggtaaaaa ggcgggtgag ttctacacgc ctcaccaagt
    17521 cagcgaagtc atggctcaga ttgtcgcaac caactcatct atttcttcta tttatgaccc
    17581 aacggttggg tcaggttccc tccttttgac cgtaaaaaaa cacctgtcgg aagataggca
    17641 gagaagtctt aactactatg gtcaggaaaa gaacacggca acctataacc tgacccgaat
    17701 gaacctcttg cttcacggtg ttcgtccgga aaaaatgagt ataaaaaatg gtgataccct
    17761 atcacaagac tggccagaag atccagaacg tccaaatgaa ggtgtccagt ttgatgcggt
    17821 ggtcatgaac ccaccttatt ctgtaaaaaa ttggaatcgt tcaggtttga aaccgagcga
    17881 cccccgtttt gaaatcgctg gtgttcttcc gccggactca aaaggcgact ttgccttcct
    17941 tttacacggt ctctatcacc tgggaactca tggaaccatg gccattgtct tgccacacgg
    18001 tgttctcttc cgcggagcag ctgagggaga aattcgcaaa cgcctcttgg aaaaaaatca
    18061 gattgatgcc gttatcggtc tgccaagcaa cctctttacc aacactggta ttccagtttg
    18121 tatcatcatc ctcaagaaaa atcgtgattt gaatgagcct gtactcttta tcgatgcctc
    18181 acgcaacttt atcaaggcag gtaaacaaaa tgccttgcaa gaaaaggata tcgcaaagat
    18241 tgtggatgtc tatacgaaca gaacggaaga agaaggttac agtcacttgg caagtcgtca
    18301 ggaattgatc caaaatgact ataacatgaa tattccacgc tatgtgactg ctctggatga
    18361 agaaattccc catgatgtgg atgcgcatct atatggtggt attccaaaga aaaatattca
    18421 gtccttgagg gtgctcaatc agacagtcaa agaggttttg gacaatgcct ttagtgaaaa
    18481 ccgtccaggc tacttaactt tgaatcagac ccttgaagag ttttcaagag tagtcttatc
    18541 cagcccagtt gttcgggcag aatatgatca agttcagtca actattgcgg cctttatcga
    18601 agaatattgg acaaaattgc atcgtttaca aacggaaacc aatacaagat tattgaaaga
    18661 agctatgttg gcagacatca agaaacgttt gtcacaattt gaccaaattg atatctacga
    18721 aggctatcaa atcattgcag agatttggac taagaccctg acccatgatg cagagttgat
    18781 tgagcaatta ggcttttatg aggctgggcg tacccgtgag ccgaatatgg tggctaaagg
    18841 gaagaacaag gagaaggttc aagatggttg gaatggggtg attatcccaa atagcttgat
    18901 tgctagtgag tgctacggtg atgagcttgc ctatattgaa agcttgaaaa atcggatttc
    18961 agaaattgac agtgaagtca gtgaattggt tgaaaatgcc aaggtggaag atagcgagga
    19021 gtataatgcc ctctttgatg tcttgaaaaa gaatgaagat ggcgaagcgc tggatagttt
    19081 tgaaggaaaa ttggtcaagg ctgctctgaa agaagcagta aaaggctcta cagaatttgg
    19141 cttcttgaag caggtagatg acctagccaa ggaaaagtct acagtagcta aggaaatcaa
    19201 gaccaaggag caggaactca aggaattggt gtccaatcgt atcccagttc tgacagatga
    19261 agaagttgac caactcctct ataaaaaatg gtttggtcag gtgacggaca aggcacttga
    19321 actggttgaa ttgccattaa aggcagaaat tgccattatc gaagaacttc acaatcgcta
    19381 tgctgaaacc ttggacgata tggatgcaga aatcgctttg cttgaaagag aacttgaaaa
    19441 tctgatgaaa gagctggtgg tgttatcatg atgaatttga aagaaatttt cttatacaaa
    19501 ccgtccaaga acaacatgcg aaaatcatca gcaacccttt tcgatcattt tccgaaaagg
    19561 gaagaaaaat cgatagaagt tgggggagtg tagaaatgcc gaacaaagaa agaaatatcc
    19621 ccaaacgtcg ctttaaagag tttgaaaatg cagatgcttg ggaactgcgg aagatttttg
    19681 aaattgcaga tagatttgat aacttaagag ttcctattgc ttctagtttg agagtttcag
    19741 gcagtacccc ttactatgga gcaaatggta tccaagatta tgttgagggg tatacacatg
    19801 agggggagtt tatcttggtt gctgaagatg gagccaatga tttaaagaac tatcctgtaa
    19861 acttagtaaa tggtaagatt tgggtgaaca accatgcaca tgtgttgcaa ggaaaaaaga
    19921 gcatcgcaga taataagttt ttggcattct caataaacca aactaacata gagtctttgt
    19981 tagttggcgg tggtagagcc aagttaaatt cggatgtcct aatgaatatt gagttaaagc
    20041 tacctagtcc agctgaacaa gaagccatcg gcactttctt ctccaccctg gaccaacaca
    20101 tcacccttca tcagcgtaag cttgacaaat taaagagtgt aaaacaagcg tatttgagtg
    20161 aaatgttccc cgctgaagga gagcgtgtgc caaaacgccg ttttccagga tttactgacg
    20221 cttgggaact gcggaagttg ggggaggtcg gaacagtcgc aatgtgtcga cgtattttta
    20281 aggaacaaac ttcggaacat ggtgaaattc ccttttataa gattggaaca tttggaggaa
    20341 cccctgacgc ctttattgct agagaattgt tcgaagaatt taaggaaaaa tattcttatc
    20401 caaatatagg ggatattttg atttctgctt cagggagtat tggtagaaca gtagaatttt
    20461 tgggtgaaga tgagtatttt caagattcaa atattgtttg gttaaatcat gatggaaaaa
    20521 ttaataatat ctttttaaaa caattttatt ccattgttaa gtggtcaggg attgaagga
//
LOCUS       DATUCG010000028        20006 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.029, whole genome shotgun sequence.
ACCESSION   DATUCG010000028 DATUCG010000000
VERSION     DATUCG010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20006
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.029"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            48..563
                     /locus_tag="VCA20_000814"
     CDS             48..563
                     /locus_tag="VCA20_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEP1834663.1"
                     /translation="MFKKYTFDPKKFRLGMRTFKSGLAVFLVILLFGYMGWEGIQIAA
                     LTAVFSLREDFDQSVHFGASRILGNSIGGFYALLFFILDRLFLDHFWVTAVFVPIFVM
                     LTIMTNVAMNNKAGIIGGVSALLIITLSIPAGDTIQYVFIRVFETFIGVFIAILVNYD
                     VNDIKKRFQDK"
     gene            639..1010
                     /locus_tag="VCA20_000815"
     CDS             639..1010
                     /locus_tag="VCA20_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEP1834664.1"
                     /translation="MREKEFRRSLAIFPIGSVMKLTDLTARQIRYYEDQGLIKPDRNE
                     GNRRLYSLNDMDLLLEIKDFLDEGLNIAAIKKEYANREAKAKVQKQQKTLTDEDVRRI
                     LHDEFSRQGRFSSPSSIFRSS"
     gene            1039..2385
                     /gene="glnA"
                     /locus_tag="VCA20_000816"
     CDS             1039..2385
                     /gene="glnA"
                     /locus_tag="VCA20_000816"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEP1834665.1"
                     /translation="MTFTVADIKRDIKEKNVTFLRLMFTDIMGVMKNVEIPATDEQVE
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWTIFPWGDENGRVAGLICDIYTTDA
                     KPFAGDPRGNLKKSLRHMEEAGFSSFNLGPEPEFFLFKMDEQGNPTLEVNDKGGYFDL
                     APTDLADNTRREIVNVLTEMGFEVEASHHEVAVGQHEIDFKYADVLKACDNIQIFKLV
                     VKTIARKHGMYATFMAKPKFGIAGSGMHCNMSLFDQDGNNAFFDPEDSRGMQLSQTAY
                     HFLGGLIKHAYNYTAITNPTVNSYKRLVPGFEAPVYIAWASKNRSPLVRVPASRGMGT
                     RLELRSVDPTANPYLAMAVLLEVGLEGIQNKIEAPAPVESNIYAMSDEERREVGITDL
                     PSTLHNALKALREDEVVRAALGDHIYTNFLEAKKIEWASYATYVSQWEIDNYLDMY"
     gene            complement(2501..4180)
                     /locus_tag="VCA20_000817"
     CDS             complement(2501..4180)
                     /locus_tag="VCA20_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEP1834666.1"
                     /translation="MSSVNLKPHEVGVFAIGGLGEIGKNTYGIEYKDEILIVDAGIKF
                     PEDDLLGIDYVIPDYSYIVENLDRVKGLVITHGHEDHIGGIPFLLKQANVPIYAGPLA
                     LALIRGKLEEHGLLRDATLHEINHNTELSFKHLKVSFFRTTHSIPEPLGIVVDTPQGK
                     IVCTGDFKFDFTPVGEPADLHRMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIM
                     KLIEGIHGRIIFASFASNIFRLQQAADAAVKTGRKIAVFGRSMEKAIVNGIELGYIKV
                     PKDTFIEPNAIKEYPASEIMILCTGSQGEPMAALSRIAHGTHRQVQLQPGDTVIFSSS
                     PIPGNTTGVNKLINILIEAGVDVIHGKINNIHTSGHGGQQEQKLMLRLIKPKYFMPVH
                     GEYRMQKIHASLAVDTGVPKDNIFIMENGDVLALTKDSARLAGQFNAQDIYVDGNRIG
                     EIGAAVLKDRRDLSEDGVVLAVATVDFNSKMILAGPDILSRGFIYMRESGELIRESQR
                     VLFNAIRIAMRNKDANIQTVNGAIVNALRPFLYEKTEREPIIIPMILTPDR"
     gene            complement(4184..4414)
                     /locus_tag="VCA20_000818"
     CDS             complement(4184..4414)
                     /locus_tag="VCA20_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEP1834667.1"
                     /translation="MIYKVFYQESKISPRREQTRALYLDIDAASELEGRIIARELVET
                     NRPEYNIEFIDLLSDNHLGYEKEHADFKITEF"
     gene            4716..5588
                     /locus_tag="VCA20_000819"
     CDS             4716..5588
                     /locus_tag="VCA20_000819"
                     /inference="COORDINATES: protein motif:HMM:TIGR01716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rgg/GadR/MutR family transcriptional regulator"
                     /protein_id="HEP1834668.1"
                     /translation="MGAIPQMSLGEFYRELRNSRLVKQKDVVRGDFTAAQLSRFESGK
                     SMLAADKLILAVEGINMTMEEFTYKYHGYKEAPHIELANLISDLYYRQDRDGLEALLE
                     SDFLKAEGNLYARLNGIVIKVALAALDGRTDFDEEDRKFLSDYLFAIEEWTSFELYLF
                     LNAQMLLNDRLLLDFAYKLCKEEKSINKLRKHQELIKIAILNTISELFERGIYHQVSY
                     YLDKLISMTGFKDVFLKIMVRYCRLLLVYIQNNRPTKEKEIIEAYIQSISEVIDEQTA
                     SIIRVHFLRFIDEK"
     gene            5863..7155
                     /locus_tag="VCA20_000820"
     CDS             5863..7155
                     /locus_tag="VCA20_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEP1834669.1"
                     /translation="MEMSPYVLRQECDDGIVYFNTKNNHSFLITKQLLEKLKTDAETK
                     EQYKTYLEQFHYFPEDDEVNQSLRKIREIDDTLLEFTILTYGDCNFRCKYCYEKFENI
                     AMSRETEDAILRFAEEQLSTGKYKVFKVSWFGGEPLLGYRTIQYLSEKFKEICRNYEV
                     HYLANITTNGYLLSLNKFKSLVEDFSIRSYQITIDGNQDSHDCQRVLKNDRGSYSRIY
                     KNLQAMKDSELDFFCTIRFNISKENKENIKSFLYGDGLYFKNDERFGFSYHNVGDWGQ
                     GDRSEKDCVTLLDYDASFELSMLANSIGYSISLVSYFLNNRFTCYANRMNHYMFNVRG
                     IVQSCTIALYHPKNVFGNINKRFINHDKLSQWVIEVDENCKSCPFVLICKSGHCPMAK
                     RISKHSSEVLCRSMQKLIKRNLALFALTKSYHDILDVG"
     gene            7322..8902
                     /locus_tag="VCA20_000821"
     CDS             7322..8902
                     /locus_tag="VCA20_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013904188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1834670.1"
                     /translation="MLGIYRYIDKKYRWLVIFGIFMNGGVMLTQPLVMSKILQLGSGD
                     LSFEKIWQLLVYGLSVYFVIYSLMLFANHTNNIFRREIHIGIRSSLFRKLISNSKYSH
                     NDKITLLTQDMELLGDRYLEPIAGFLCWGFVSFVTALYIIMQDILLGSIFVVCTILRP
                     VPQFLLNKRLKNTGMEFSELRTKVHEEVSDSIHGSETLLVNQSAENSFHRVGQIVKEC
                     QVAIQRYAFTHNIVFFFNGFMEFFSQILPVAIGFYLIMNGEAITVANLLTMFIAANRL
                     AGPIQNLMYQAEGIQGAQAIADKIFGILGTEPDFVERKESHLSDIQGLYMKDLAKTYG
                     DKQLFAGLNVAIKMGEKALIKGASGSGKTTLFRILLGQETADGGRVYVTEAGGQERED
                     FQGNIGLISQSPFLFSDTIRYNLTLGDNFTDQELLDVLSKVGLLDEFTDILNVKVENN
                     GDNISGGQRVRLEIARFLLRKKDILLADEVTAALDEKNGKAVRKLLHSLPIMILEIAH
                     HIDEDIQYDQVVELKREE"
     gene            8998..9681
                     /gene="tsaB"
                     /locus_tag="VCA20_000822"
     CDS             8998..9681
                     /gene="tsaB"
                     /locus_tag="VCA20_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEP1834671.1"
                     /translation="MKILALDSSNQALSVALVEDGRLQAETLLAVKKNHSISLMPVVD
                     FLVAQVGWTPKDLDRIVVAQGPGSYTGLRVAVATAKTLAYTLKIDLVGLSSLQSLVPP
                     SLTGLVVPLIDARRNCVYAGVYENGKAVEPDRYWSFEDLLSSLAGKENITFVGEVENF
                     MEQIEQALPTAQYQASLPSAYQLAVIGQDLPAVDVTSFEPNYLKRVEAEENWLKDNQV
                     SSESYIKRV"
     gene            9678..10118
                     /gene="rimI"
                     /locus_tag="VCA20_000823"
     CDS             9678..10118
                     /gene="rimI"
                     /locus_tag="VCA20_000823"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEP1834672.1"
                     /translation="MIEIKHYDGQENLAEAVLAVMESVYDQSPWTLEQIASSMTSQDE
                     DYYLAYEGQELVGFLAVQTVLDEMEILQIAVKADFQRLGIASQLMAAVMDWEGDIFLE
                     VRESNSAAQALYTRQHFTKIGKRKDYYRHPVEDAVMMKREPDER"
     gene            10108..11115
                     /gene="tsaD"
                     /locus_tag="VCA20_000824"
     CDS             10108..11115
                     /gene="tsaD"
                     /locus_tag="VCA20_000824"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEP1834673.1"
                     /translation="MKDRLILAIETSCDETSVAVLRNDAELLSNVIASQIASHQRFGG
                     VVPEVASRHHVEVITACIKEALLEANVTAEDLTAVAVTYGPGLVGALLVGISAAKAFA
                     WANGLPLIPVNHMAGHLMAARAVKELEFPLLALLVSGGHTELVYVSEAGDYKIVGETR
                     DDAVGEAYDKVGRVMGLPYPAGRVIDELAHEGQDIYDFPRAMIKEDNLEFSFSGLKSA
                     FINLYHNAQQKGESLSNADLSASFQACVMDILMAKTKKALEKYPVKTLVVAGGVAANQ
                     GLRERLAAEITDVEVIIPPLRLCGDNAGMIALAAVSEYNKENLAGWDLNAKPSLAFEN
                     L"
     gene            complement(11152..11988)
                     /locus_tag="VCA20_000825"
     CDS             complement(11152..11988)
                     /locus_tag="VCA20_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEP1834674.1"
                     /translation="MKIQPEFYVFNNRSYVDFYPIQFGMEACKPLHSFGPTMKQHYLF
                     HYIISGSGTFYDTSDQREYHLTAGQGFLISPDAICSYEADEKDPWTYIWLEFDGLKTE
                     HFLRQAGLSKEQPIFSQREMPTSSPVYHEMKQILALHHQKSACLLGHLYLFISYLIDC
                     SLTKTSSKHDENKEFYIREAINFVERNYEKAISVDDLAQVCNLNRHYFSRLFKEQMNI
                     SPQQFIIQYRLSEACELLKNTTKTLQEIAEEIGYSNQFNFSTAFKRHYQISPNRWRKI
                     HK"
     gene            12203..13477
                     /locus_tag="VCA20_000826"
     CDS             12203..13477
                     /locus_tag="VCA20_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEP1834675.1"
                     /translation="MKKIGRYVTLLAAAGLLAACSTSTESSKTTGGSSSGKTEISYAI
                     WDSGQEPGLRKIADEFEKKNPDIKINIQVSDWDSYWTMLEAGATGGSLPDTFWMHSNE
                     IYRYGSNEMLLPLDEYLAKSEDAKLANFPDGLNEIYNINGKQYAIPKDFDTIGLWYNK
                     KLFDEAGIPYPDDTWDWNKLKEVAKKLTKPDGSQYGFGAGLSNQEGYYNFIYQNGGKV
                     ITDDLKSGYADPKTIEALDYYFSFVKEKISPAITVDKERAEAFQNGQVAMSVFGSWNL
                     SGFTANDYIRENADVAVLPKGPDGTRATIFNGLGHAIAATTKHPDAAWKWVEYLSSKE
                     AQEMQATLGVAISAYKGAADTWVDSNKNFTIKNYVDMVDYAQIRPYSQTTIKWEDKAY
                     ELLKPAYLGEKATDEAAKETADMMNAELATEK"
     gene            13546..14439
                     /locus_tag="VCA20_000827"
     CDS             13546..14439
                     /locus_tag="VCA20_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEP1834676.1"
                     /translation="MFRKKGSLNEAIWGWGMVAPTIIGLIVLNIIPIFQTMKMSFHKS
                     GDFGKGDIFVGLANYQRMLGDVQVWQATWNTLKYTILVVPATVALAMLLAVLLNSKIK
                     GKHIYRTIFFLPMVAAPAAVTMVWKWLYNTDFGLINYILRRLGLGAVNWIEDPKIALY
                     SIALIGIWSTVGYSMILILAGLQEIPTDFYEAARIDGASPVKQFFSITLPLVSPTLFF
                     VVVTSVIQSMQVFDVIYMMEDVRSPAYDKTVSLVYLFYNNSFKYSDKGYGSTIVMLLL
                     LIILVITFVQMKVQKKWVHYR"
     gene            14450..15280
                     /locus_tag="VCA20_000828"
     CDS             14450..15280
                     /locus_tag="VCA20_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEP1834677.1"
                     /translation="MKQSAFKSTIWMHAFLILVAMGMLIPFVWMVLTSFKTVTESTQM
                     NPFQFLPSNWMVSNYTEAIRTNNFPILYLNTILMMLWRIFSSVMFSAMAAYAFARLEF
                     PGRNFLFGLVLFQMMVPPQLFVIPQYLMIDQLGMRNTIFALVFPGIVSAFGTFLLRQF
                     FMGLPKELEESAKLDGCNIGQTFFKVMLPLAKSGLIALAIFTALFAFKDLLWPLIINS
                     EADKATLSSALSKIQGAYAVNYPQLMAASVLAIWPMLVLYVIFQKQFIQGIATSGGKL
                     "
     gene            15290..17491
                     /locus_tag="VCA20_000829"
     CDS             15290..17491
                     /locus_tag="VCA20_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEP1834678.1"
                     /translation="MGIIFHEKTKEFHLYNEFISYVLTVLPNGHIGSLYYGKRVTETA
                     SYQYVREDEYRALTSFVEDDDRFFSLQYANPEFACYGTTDYFSPTFELVQKDGSSLSH
                     FVYQGHVIYAGRSALKGLPHLYLDEEGQADSLDIYLLDEKSQTRLVLSYTIFRDYPAM
                     TRSARFEQLGEKSVCLNRALSMTLCLPDMDYDWLHLDGAWGRERHLQLSPLHQGCQSI
                     YSLKGASSAEHNPFMALKRPTADEQQGEVLGFSLVYSGNFLAQVDVSSFQKTRVSMGI
                     HPERFYWTLDKGEEFQTPEVVMVYSEHGLNGMSQTYHRLFQKHLVRGYWRDRERPVLL
                     NNWEAMSFDFDEETILSLAKEAAGLGVELFVMDDGWFGKRNHDRAGLGDWTVNREKLP
                     SGLTGIIDQVHAMGMKFGLWIEPEMVNRDSDLYRAHPDWIFHHPQHSQSHGRHQYTLD
                     LSREDVYQNIHDQLHWLLAEHDIDYIKWDMNRYMTEVFTCTREADRQGETFHRYILNL
                     YRLYDSLIAAFPTVLFESCSSGGARFDPGMLYYAPQTWVSDDTDAMERLKIQYGTSIV
                     YPLNSMGCHVSASPNQQLGRATPLNTRANVAFFGSFGYELDLFECSQEELEEMKEQIA
                     FYKTHRKIFQQGIFTRLKSPFDGEITAWQVQSSDGQQVIVGYYRRLTTANLAYQRLYL
                     QGLEEEAVYHLDGEVYTGSQLMHTGLSIRHGDHVGDNKDFTSCLMVLQKQQ"
     gene            17766..18467
                     /locus_tag="VCA20_000830"
     CDS             17766..18467
                     /locus_tag="VCA20_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEP1834679.1"
                     /translation="MKKYAFREGMRDAIPTVLGYASIGLACGVVSVNSGISTVEMGLM
                     SLLIYAGSAQFVMCAMIFAGAPLLSIAVTVFFVNLRNFLMCLHATTIFQGNRLGSNIL
                     IGSFVTDESYAVLLRKQIEHHTITPEWMYGNNFASYASWVTFTTLGNLIGGLIPNPEQ
                     FGLDFALVAMFVGIFAGQLEAMVRTIPLKKIGLILLAVGLGYIGLSMMVSSYVAVLLA
                     TLLGCFVGVMIDDKN"
     gene            18454..18777
                     /locus_tag="VCA20_000831"
     CDS             18454..18777
                     /locus_tag="VCA20_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEP1834680.1"
                     /translation="MIKTNILLIILMTVLVTWVPRVLPFVLTQNKSLPPKLVKFLSFL
                     PITIIFTLTLSSIMDEKVGSLPSFLPVESLAILPTFFVVLKTKNILLAVLVGVVTTAV
                     LRLIF"
     gene            18787..19809
                     /locus_tag="VCA20_000832"
     CDS             18787..19809
                     /locus_tag="VCA20_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HEP1834681.1"
                     /translation="MVKENAKGVGLCFLLALVGQWLGHLFPLVGGPVFALLIGMSLYP
                     YFSSKNAFHSGLTFTSKKILQYAVICLGFGLNLSAVLAVGRQSLPIILSTISFALFLA
                     FLMWKWLPISSHLATLIGVGTSICGGSAIAATAPIIQADDEDVAQAISVIFLFNVLAA
                     LVFPTLATWLGVSTDSGHAFGMFAGTAVNDTSSVTAAASTWDSLYGLGSQTLDTAVTV
                     KLTRTLAIIPITTVLAIWQTRGKGLKADKKSLLTGFPTFILYFILASLITTIAGGFGI
                     GADWFAPLKSLSKFFICMAMVAIGLRTNVFALVKNGRAALLVGLVCWLGVTVLTLVWQ
                     AILGIW"
BASE COUNT         5411 a         3581 c         4845 g         6169 t
ORIGIN      
        1 gcaggtattt gctattctat tttccgaaat catgttagaa tagaggtatg tttaaaaaat
       61 atacatttga tccaaagaaa tttcggttgg ggatgcggac gttcaaatcg ggtttggcag
      121 tctttctagt tattctactc tttggctata tgggctggga agggattcag attgcggctt
      181 tgacggctgt gtttagttta cgagaagact tcgatcagag tgtccatttt ggagcttctc
      241 gtattctggg taattctatc ggcggttttt atgcgctact tttctttatt ttggataggt
      301 tattcttaga tcatttttgg gtaactgcgg tttttgttcc gatatttgtc atgttaacga
      361 ttatgacaaa tgtggcgatg aataataaag cgggaattat tggtggtgta tcagctttat
      421 taattattac attgtctatt cctgctggtg atacgattca atatgtgttt attcgtgtgt
      481 ttgaaacctt tatcggagtt tttattgcga ttctagttaa ttatgatgtg aatgatataa
      541 aaaaacgttt tcaggataaa taatgttata aaatgtaaca tcgcctattg acctcaaaaa
      601 aaaattctcc tataatggta ggcagaaagg agttcgttat gagagaaaaa gagtttcgtc
      661 gttccttagc catctttccg atcggtagtg tgatgaagtt gacagattta actgctcgtc
      721 aaattcgtta ctatgaagat caaggcttga ttaagcccga tcgcaacgaa gggaatcgtc
      781 gcttgtattc tctaaacgat atggacttac ttttagaaat caaggacttt ttagatgaag
      841 gtttgaatat tgctgcgatt aagaaggaat atgcaaatcg ggaagcgaaa gctaaggtcc
      901 aaaaacagca gaaaaccttg actgatgaag atgtccgtcg tatcttacat gatgaattca
      961 gtcgtcaggg acgattttca agtcctagtt ccattttccg ttcgtcgtaa ttagctttta
     1021 aaatagagga gaaaaataat gacattcaca gtggcagata tcaagcgtga tatcaaagag
     1081 aaaaacgtta ccttcctacg tcttatgttt acggacatca tgggagtgat gaaaaacgta
     1141 gaaattccag caactgatga gcaggtcgaa aaggttttgt caaataaggt catgtttgat
     1201 ggttcatcta ttgaaggatt tgttcgtatc aatgaatcag atatgtatct ttacccagac
     1261 ttggatacat ggactatctt cccttggggt gatgaaaatg gccgtgtagc tggcttgatt
     1321 tgtgatattt atacaacaga tgctaagcct tttgctggtg acccacgtgg aaatttgaag
     1381 aaatcgcttc gtcacatgga agaggcaggt ttctcttcat tcaaccttgg tccagaacca
     1441 gaattcttcc tctttaagat ggacgaacaa ggaaatccaa cgcttgaagt aaatgataaa
     1501 ggtggttact ttgacctcgc accaacggac ttggctgata atacacgtcg cgaaatcgtc
     1561 aatgtcttga cagagatggg ctttgaagtt gaagcgagcc atcacgaagt ggcagtaggt
     1621 cagcatgaaa ttgacttcaa atatgcggat gtcttaaaag cttgtgacaa tattcaaatc
     1681 ttcaagttag ttgttaaaac aattgctcgt aagcatggta tgtatgcgac atttatggcc
     1741 aagcctaagt ttggtattgc tggttcaggt atgcactgta acatgtcact ctttgaccag
     1801 gatggaaaca atgccttctt tgatccagag gattctcgcg gtatgcagct gtctcaaact
     1861 gcttaccatt tccttggtgg tttgattaag catgcttata actatactgc tattacaaac
     1921 ccgacagtga actcttacaa gcgtttggtt ccaggctttg aagctcctgt gtatattgct
     1981 tgggctagta aaaaccgctc accattggtt cgcgtacctg catcacgtgg aatgggaact
     2041 cgtttggaat tgcgctcggt tgatccaact gccaacccat acttagctat ggcagttcta
     2101 ttggaagttg gtttggaagg tattcaaaat aaaattgaag caccagcacc agtagaatct
     2161 aatatctatg caatgtctga tgaggagcgt cgtgaagtag gtatcactga tcttccttca
     2221 actcttcaca atgcattgaa agctcttcgt gaagacgagg tagttcgtgc agctctaggt
     2281 gatcatatct atacgaattt cttggaagct aagaaaatcg aatgggcaag ctacgcaacc
     2341 tacgtttctc aatgggaaat tgacaactat ttggatatgt attaaaaaat ggtccggtgg
     2401 accatttttt catgagcttg aaatctgtct tttttcaagc tgggttatct tgttaatata
     2461 aaataaatcc cagaggtctc acctctggga ttttagtgtg ttatcggtca ggcgtcagaa
     2521 tcattgggat aatgattggt tcacgctctg ttttttcgta taggaatggt cgtagggcgt
     2581 taacaatggc gccgttgact gtttggatat ttgcgtcttt gttgcgcatg gcgatgcgga
     2641 tagcgttgaa aaggacgcgt tggctttcgc gaatgagctc gccagattct ctcatataga
     2701 taaaaccgcg actgaggatg tctggccctg ctaatatcat cttagaatta aaatcgacag
     2761 tggctactgc aaggacaaca ccatcttctg aaagatcgcg gcggtctttt agaacagctg
     2821 cgccgatttc accgatgcgg tttccatcga catagatgtc ttgggcgttg aattgacctg
     2881 cgagacgtgc agaatctttt gtcagagcaa ggacatctcc gttttccatg atgaagatat
     2941 tatctttggg aacgcctgta tcaacagcta ggctggcatg gattttttgc atgcggtatt
     3001 caccatggac aggcatgaag tattttggtt tgatcaagcg gagcatgagt ttttgctctt
     3061 gttgaccacc gtgcccagag gtatggatgt tgttgatctt gccgtgaatt acatcgacac
     3121 cggcttcaat caagatattg atgagcttgt tgacacctgt ggtatttccc ggaataggac
     3181 tggacgagaa gataaccgtg tcgcctggct ggagttgcac ttgacggtgg gtaccgtggg
     3241 cgatgcgaga gagggctgcc ataggctccc cttggctacc tgtacagaga atcataattt
     3301 cgctggcagg gtattctttg atcgcgtttg gctcgatgaa ggtatcctta ggcaccttga
     3361 tgtagccgag ttcgataccg ttaacaatgg ctttttccat ggagcgtcca aaaacagcta
     3421 tcttgcgtcc ggtcttaacc gctgcatcag ccgcctgttg gagacggaag atattggagg
     3481 caaaggaagc aaagatgatg cgtccgtgaa tgccttcaat cagcttcata atggactgac
     3541 cgaccacttt ttccgagttg gtgaaggttg ggacttcagc atttgtcgag tcggagagga
     3601 ggcagagaac gccttcttca ccgagggcag ccatgcgatg caggtctgct ggctcgccaa
     3661 ctggagtgaa gtcgaacttg aagtcaccgg tacagacgat ttttccttgc ggggtgtcaa
     3721 cgacgatacc aagtggttct ggaatggagt gggtggtgcg gaagaagctg accttgaggt
     3781 gtttgaagct caactctgta ttatgattga tttcatgcaa ggtcgcatca cggagcagtc
     3841 cgtgttcttc caatttgcct cgaataaggg cgagggctaa aggtccagca tagattggga
     3901 cattggcttg cttgagtaag aagggaatgc ctccgatgtg gtcttcgtgc ccgtgggtaa
     3961 taaccagacc tttaacgcgg tctaggtttt caacgatgta ggagtagtct gggataacgt
     4021 agtcaatacc caataggtca tcttctggga acttgatacc ggcatcgacg atgagaattt
     4081 catccttata ctcgatacca taggtatttt tcccgatttc tcccaaacca ccgatggcga
     4141 aaacgccaac ttcatgaggt tttagattga ctgatgacat ggcttaaaac tccgtaattt
     4201 tgaaatctgc gtgctctttc tcgtatccaa gatgattgtc tgataagagg tcaataaatt
     4261 cgatgttgta ttcagggcgg ttggtttcca ccaattcacg cgcgataata cgaccttcca
     4321 attctgatgc agcatcgatg tctagataaa gagcacgtgt ttgctcgcga cgtgggctaa
     4381 tttttgattc ttgatagaat actttataaa tcatgtgtag tatagttcct ttctttttga
     4441 ctggcaacta tgaaaaaagc cccaaacagt tggggctgtc tggtaatgcc tattcaattt
     4501 tctcgagttg tcaatttatt cagtctaaat gtactcttca ttataccata aaatatgctg
     4561 atggtaaaag ggtttggtag gaaaattgaa aagtgtcatg attttcagaa acaagcagtc
     4621 atttggcaga ataataagtg aaattgacaa aaatatattt ggatattata ataaatgtaa
     4681 gcggattttc aatgcatcaa gaaaggggat gagtcatggg tgcaatacca caaatgagtt
     4741 taggtgaatt ttacagggaa ctccgaaatt ctcgcttggt taagcaaaag gatgtcgtca
     4801 gaggagactt tacagcagcc cagctatcac gctttgaatc gggcaagtcc atgttggcgg
     4861 cagataagtt gatactggca gttgaaggga ttaacatgac catggaggag tttacctata
     4921 aataccatgg gtacaaggaa gcgcctcata ttgagctggc taatctgatt tcagatttgt
     4981 attaccgaca ggatagggat ggactagagg ctttgttgga gtcagacttc ttgaaagctg
     5041 agggaaatct ctatgctagg ttaaatggaa tagtcatcaa ggtggcgctg gctgccttag
     5101 atggacgaac tgattttgac gaggaggata ggaagtttct ctcagattat ctctttgcta
     5161 ttgaggagtg gaccagtttt gagctctatt tatttttgaa tgcacaaatg ttgttgaatg
     5221 ataggttgtt gctggatttt gcttataaat tatgtaagga agaaaaatct attaataaac
     5281 ttagaaagca ccaagaactt ataaaaattg cgattctaaa tacgatttcg gaattatttg
     5341 aaagaggaat ctatcatcag gtttcttatt acttggataa actcatttct atgactggat
     5401 ttaaagatgt tttcttgaag ataatggtac gatattgccg gttattacta gtttacatcc
     5461 aaaataatcg tcccactaaa gaaaaagaaa taatcgaagc ttatatacaa tctatcagcg
     5521 aggtgataga cgaacaaaca gcttcgatta tccgagtaca ttttttaaga tttatagatg
     5581 aaaagtgaca ggaataggag gcagtggttt agataaggca tcaattacaa atggaagtga
     5641 cagtgagata gcgaaaataa tatatacaac tttctgtttc atggctttgt acctcgtttt
     5701 tattctcagt gtaactgatt tttcctcggt tatcattaac ttgcaaatat gtaatttatt
     5761 tttataaaat tacatatttg caagtttcaa tagtaccaat tgatttgtgt tagaatttag
     5821 acaagaaata ataaatgaaa tcaaagacag ggaggtatgc gtatggagat gtcaccctat
     5881 gttctccgtc aggaatgtga cgatggtatt gtctatttca atacaaagaa taatcattca
     5941 tttctaatca cgaaacaact gttagaaaaa ttgaagactg acgcggaaac caaagagcaa
     6001 tataagacct accttgaaca gtttcattat tttccagagg acgatgaagt aaatcagtcg
     6061 ttaaggaaga tacgtgagat agacgacact ttattagagt ttacaatttt aacgtatggt
     6121 gattgtaatt ttcgctgtaa atattgttat gaaaaatttg aaaatattgc tatgtcaaga
     6181 gagacagagg atgcaattct gagatttgca gaagagcagc tgagtactgg aaagtataag
     6241 gttttcaaag tttcatggtt tggtggtgag ccactgctcg gatatcgtac gattcaatat
     6301 ctttctgaaa aattcaaaga aatttgtcga aattatgagg tacactattt agcaaacatt
     6361 actacaaatg gttatctctt atcgttgaat aaatttaagt ctttggttga agatttcagt
     6421 attcgtagtt atcaaataac gatagatggt aatcaagata gtcatgattg ccagagggtc
     6481 ttgaaaaacg atagagggtc atacagtcgt atttataaaa atttacaggc aatgaaagat
     6541 agtgagttag attttttctg cacgattcga tttaacattt caaaagagaa taaagaaaat
     6601 ataaaatctt ttctttatgg agatggtttg tattttaaga atgatgaacg gtttggcttt
     6661 tcttaccata atgttggtga ttgggggcaa ggagatcgct cggagaaaga ttgtgtgact
     6721 ttacttgatt atgatgcgag ttttgaacta tcaatgctag caaattccat tggttatagt
     6781 atttccttag ttagttattt tttaaataac cgctttactt gttatgccaa tcggatgaat
     6841 cactatatgt ttaatgtaag aggtattgtg caatcttgca caatcgcatt atatcatccc
     6901 aaaaatgtat ttggtaatat aaataagagg tttattaatc atgataaatt gagccaatgg
     6961 gtgattgaag tggatgaaaa ctgtaaatca tgcccatttg tcttaatttg taagtcaggt
     7021 cattgcccaa tggctaagcg aatatcaaaa cactcttctg aggtgctctg tcgtagtatg
     7081 cagaagttaa taaaaagaaa tctagcacta ttcgcattga ctaagtccta tcatgatatt
     7141 ttagatgtgg gttagaagga ggtatttatg gctgtaaata ataataaagt ttatgtagaa
     7201 gaagatattc tactggctgc ctccaataac ggatgtaatt caccgtgtac actaccgtag
     7261 atgctatatg agaggagtgc tagcctctct ttttgctacg aggaggttgc ttatgaagag
     7321 aattcttgga atttatcgtt atattgataa aaaatacaga tggttggtga tatttggtat
     7381 ctttatgaat ggcggagtta tgctgactca gcccttagta atgagcaaga tattgcaact
     7441 cgggagcggg gatttaagtt ttgaaaaaat ttggcaacta ttagtgtatg gtttgtctgt
     7501 ttattttgtg atttatagtt tgatgctttt cgccaatcat acaaataata tatttagaag
     7561 agaaattcat atcggaattc gctcgtcctt atttagaaag ctgatttcaa attctaaata
     7621 tagccataat gataagataa ctctattaac tcaggatatg gaattgttgg gggataggta
     7681 tcttgaaccg attgcaggtt tcctatgttg ggggtttgtc agcttcgtta ctgcccttta
     7741 catcataatg caggacattt tgctggggtc tatttttgtg gtgtgtacga ttttgagacc
     7801 agttccacaa tttttactaa ataaaagatt gaaaaatact gggatggagt tttcagaact
     7861 tcgaactaaa gttcatgagg aagtatccga tagtatccat ggtagcgaaa cgcttcttgt
     7921 gaatcaaagt gctgaaaata gttttcatcg ggtaggtcaa atagttaaag agtgtcaagt
     7981 agccatacag cgatatgctt ttacccacaa tattgtcttt ttctttaatg gttttatgga
     8041 gtttttcagc cagatattgc ctgttgccat tggtttttac ttgattatga atggtgaagc
     8101 cattacggtc gctaacttgc tgactatgtt tattgctgcc aaccgtttgg cgggtccgat
     8161 tcagaatttg atgtatcagg cggaaggcat ccaaggagct caagctattg cagataaaat
     8221 ctttggcatt ttgggaacag agcctgattt tgtggagcgt aaagaaagtc atctgtctga
     8281 cattcagggc ttgtatatga aagatttagc caaaacctat ggcgataagc aactgtttgc
     8341 ggggttgaat gtggctatca aaatgggaga aaaggcctta atcaaaggtg ccagtggttc
     8401 tggtaaaaca acgctttttc ggattttgct agggcaagaa acagcggatg gaggtcgtgt
     8461 ttatgtgaca gaggctggtg gtcaggagcg agaggatttt cagggcaata tcggtctgat
     8521 tagccaaagt ccgtttttat ttagcgatac tattcgttac aatctgaccc tgggtgataa
     8581 ttttacagac caagaactgt tggatgttct gagcaaggtt gggcttcttg atgaatttac
     8641 agatattctc aatgtgaagg tagaaaataa tggtgacaat atctcaggtg gtcaacgggt
     8701 ccgtctggaa attgctcgtt tcctcctgcg gaagaaggac attctcctag cagatgaggt
     8761 aactgcagcc ttggatgaga aaaatggcaa ggctgttcgc aaactcctac attctttacc
     8821 gattatgata ttggaaattg cccaccatat cgacgaggat attcaatatg atcaagtggt
     8881 agagttgaaa agagaagaat gacagactgg gaaatcatac cccagtcttt tcattatttg
     8941 aaaaaaactt caatatagct tacaataggg agtagcatta aaaagaaaga gaaatttatg
     9001 aaaatactag ctttagacag ctctaaccaa gccttgtcgg tggccttggt ggaggacggt
     9061 cgtttgcagg cggaaaccct gttggctgtc aagaaaaatc atagcatcag cctcatgcct
     9121 gtggtcgatt ttttggtggc gcaggtgggc tggacgccca aagatttgga tcggattgtg
     9181 gtcgctcagg gacctggctc ctacacgggt ctgcgggtgg cggtggcgac tgccaagacc
     9241 ctggcctaca ccctcaagat tgacttggtg gggctatcca gtcttcagtc tctggttcct
     9301 cctagcctga cgggtctggt ggtgcccctt atcgatgccc gccgtaactg cgtctatgcc
     9361 ggtgtttatg aaaatggcaa agcagtcgag ccagaccgct actggtcttt tgaagacttg
     9421 ctttctagcc tggctggcaa agaaaacatc acctttgtcg gagaagtaga aaactttatg
     9481 gagcaaatcg agcaagctct gccgactgct cagtatcaag ccagtctgcc gtctgcctat
     9541 cagctagcag taatcggtca agacttgcca gcagtagatg tgacgagctt tgagcccaac
     9601 tatctcaagc gtgtagaggc agaagaaaac tggctcaagg acaaccaagt tagctcagaa
     9661 agttatatca agcgtgtatg atagagatta aacactacga cggtcaggaa aatctggcgg
     9721 aggctgtgtt ggcagttatg gagtcggtct atgatcagtc accttggacc ttggaacaga
     9781 ttgcttcaag catgaccagt caggatgagg attattatct ggcctatgag ggtcaggaat
     9841 tggtcggatt tttggctgtt cagacggtgc tggatgagat ggaaatcttg cagattgctg
     9901 tcaaggctga ttttcagagg ttgggaatcg ccagtcaact gatggctgct gtgatggact
     9961 gggaggggga catcttcctc gaagtgaggg agtccaatag tgcagctcag gctctctata
    10021 cacgccagca ttttaccaaa ataggaaaac gaaaagacta ctaccgccat cctgtcgagg
    10081 acgcggtgat gatgaagaga gaacctgatg aaagatagat tgattttggc gattgagacc
    10141 tcctgtgacg agacctcggt ggctgtttta cgaaatgacg cggagctttt atccaatgtc
    10201 attgctagcc agattgccag ccaccagcgg tttggtgggg tggtgcctga agtggccagc
    10261 cgtcaccatg tggaagtgat tacggcttgt atcaaggagg ctttgttgga ggcaaatgtg
    10321 acggcagagg acctgacggc tgtggctgtg acctatgggc ctggcttggt cggtgccttg
    10381 ctagtcggga tttcggctgc caaggcattt gcttgggcta acggtctgcc gctcattcct
    10441 gtcaaccata tggcagggca tttgatggcg gcgcgtgcgg tcaaggagtt ggagtttcca
    10501 cttttggctc ttttggtcag cggtgggcac acggagttgg tctatgtgtc agaagcgggc
    10561 gactacaaga tcgtcggtga aacgcgagat gatgcggtcg gcgaagccta tgacaaggtg
    10621 gggcgggtca tgggtctgcc ctatccagct ggtcgggtga ttgatgagtt ggcacatgag
    10681 gggcaggaca tttatgactt ccctcgtgct atgatcaagg aagataatct ggaattttct
    10741 ttctcaggtt tgaaatccgc ctttatcaac ctctaccaca atgctcagca gaaaggggaa
    10801 agcttgtcca atgcggacct gtcagcctct ttccaagcct gtgtcatgga tattctcatg
    10861 gctaagacca agaaggcctt ggagaaatac cctgtcaaga ccttggtggt agcgggcggt
    10921 gtggcagcca atcagggctt acgagagcgg ttggcagctg aaatcaccga tgtagaggtc
    10981 atcattcccc cgcttcgcct ctgcggtgac aatgcaggca tgattgcctt ggcagcggtc
    11041 agtgagtaca ataaggaaaa ccttgctggc tgggacctca atgccaagcc tagtctggct
    11101 tttgagaatt tatagaataa aaacctctta cgtcaaggaa acgtaagagg tttatttgtg
    11161 gatttttcgc cagcgatttg gagaaatttg gtaatgtctt ttaaaagcag ttgaaaaatt
    11221 gaattggttg gagtagccga tttcttcagc aatttcctgt agtgtcttag tggtgttttt
    11281 taagagttcg caggcttcgc tgagtcggta ttggatgata aattgttgag gtgagatgtt
    11341 catttgctcc ttgaatagcc gactgaagta gtggcgattg aggttgcata cttgtgccaa
    11401 atcatcgacg gatatagctt tctcataatt tcgttctacg aagttgatgg cctctcttat
    11461 ataaaattcc ttgttttcat cgtgtttact gcttgttttg gtcagagagc agtcgatgag
    11521 ataggagata aagaggtaga ggtgcccaag aaggcaggcg cttttttgat gatggagcgc
    11581 gagaatttgt ttcatttcat ggtagacagg actagaggtg ggcatttctc gttgtgaaaa
    11641 gataggttgt tctttcgaga gccctgcctg tctgagaaaa tgctcagttt tcagaccgtc
    11701 aaattcaagc cagatatagg tccaggggtc cttttcgtct gcttcgtagc tacagatggc
    11761 gtctggggag ataaggaagc cctgtcctgc ggttaggtgg tattcacgtt ggtcggaggt
    11821 atcgtaaaaa gtacctgaac cagagatgat gtagtgaaac aggtagtgtt gtttcatagt
    11881 tgggccgaat gagtgaagtg gcttacaggc ttccatgcca aattggatgg ggtaaaaatc
    11941 tacataggat ctgttattaa agacgtaaaa ttcaggttgt attttcatcg tattctccat
    12001 accaaaagtg taaaggctta caatcagtat agcatattta tatgtaaatg tgacatttta
    12061 tttgaaaaaa ttaaaaaata tcccaaaatc atatggaata tgtcaaatat tcctatttga
    12121 ttataaaacg cttacatgtt atagttatag tagaaataac aaagttcgtc gacgtattcg
    12181 acaaaagaaa aggagtatta tcatgaagaa aattggtcgc tatgttacct tgcttgcggc
    12241 tgcaggcctg ctggcagcgt gttctacttc gactgagtct agcaaaacta cgggaggaag
    12301 tagttcagga aagacagaaa tatcctatgc tatttgggat tctggtcaag agcctggttt
    12361 gagaaagatt gcggatgagt ttgagaaaaa gaatccagat attaaaatca atattcaagt
    12421 atctgactgg gattcttact ggaccatgct agaagctggt gcgacaggtg gttcattgcc
    12481 tgataccttc tggatgcatt caaatgagat ttatcgctat ggttccaatg aaatgctatt
    12541 gccattggat gaatacttag ccaagagtga agatgctaaa ctagctaatt tcccggatgg
    12601 tttgaatgaa atctacaaca taaatggtaa gcagtacgct attccaaagg atttcgatac
    12661 aatcggtctt tggtacaata aaaaattatt tgacgaagca ggcattccat atccagatga
    12721 tacttgggat tggaataagt tgaaagaagt agcgaagaaa ttgaccaagc cagatggtag
    12781 tcaatatggt ttcggtgctg gtttgagtaa ccaagagggc tactacaact ttatctatca
    12841 aaatggtggt aaggtcatta ctgatgattt gaaatcaggc tatgctgatc ctaagaccat
    12901 tgaagccttg gattactact ttagctttgt caaagaaaaa atttcaccag ctattacagt
    12961 agataaagag cgggccgaag cattccaaaa cggtcaagtg gctatgagcg tcttcggttc
    13021 ttggaattta tctggtttca ctgctaatga ctacattcgt gaaaatgctg atgtagctgt
    13081 tttgccaaaa ggtccagatg gaactcgtgc aaccatcttt aatggattgg gtcatgccat
    13141 tgctgctacc actaaacatc cagatgctgc ttggaaatgg gttgaatacc tcagctcaaa
    13201 agaggcacaa gaaatgcagg caacacttgg tgtggcaatc tcggcttata aaggcgcggc
    13261 agatacttgg gtagattcaa acaaaaactt tactatcaag aattatgttg acatggtgga
    13321 ttatgcacag attcgtccat attcacaaac aaccatcaag tgggaagata aggcttatga
    13381 gctattgaaa cctgcttatc ttggagaaaa agcaactgac gaagctgcca aagaaacagc
    13441 tgatatgatg aatgctgaat tagcaactga aaaataggta ggaggtcttg ggaagttgat
    13501 tcttcccctg accttcttat taaaatgaag aaggatgggg ataagatgtt ccgaaaaaaa
    13561 ggaagtttaa atgaggcaat ttggggatgg ggaatggtag caccgaccat tattggatta
    13621 atcgtgctca atattattcc tattttccaa accatgaaga tgagttttca taagagtgga
    13681 gatttcggta aaggcgatat ttttgtcggc ttggcaaatt accaacgaat gctaggggat
    13741 gtgcaagtgt ggcaggcgac atggaatacc ttgaaatata cgattctagt tgttccagct
    13801 accgttgcct tggccatgtt gcttgcagtt ttgcttaatt ctaagattaa aggtaagcat
    13861 atttatcgaa ccattttctt cttgcctatg gtggcagcac cagccgcggt gaccatggtt
    13921 tggaaatggc tctacaatac ggacttcggt ctgattaact atatcttgcg tcgtttggga
    13981 ctgggtgctg tgaactggat tgaagatcca aagattgctc tctattcaat tgctctgatt
    14041 ggaatttgga gtactgttgg ctatagcatg attttaattt tagctggttt gcaagaaatt
    14101 ccaacggatt tttatgaggc agctcgcatt gatggagcta gtccagtcaa gcaatttttc
    14161 tccattaccc tgccattggt ttcaccaacc ctgttctttg ttgtggtaac cagtgtcatt
    14221 caatccatgc aagtctttga tgttatctat atgatggagg atgttcgtag tcctgcctat
    14281 gataagacag tatctctggt ttatctgttt tacaataatt cctttaaata ttcggataag
    14341 ggctatggct ccaccattgt catgttgttg ttattaatta ttcttgtcat tactttcgtt
    14401 cagatgaagg tacagaaaaa atgggtgcat taccgttaag gaggttcatg tgaaacaatc
    14461 agcttttaaa tcaacaatct ggatgcatgc cttcttgatt ttagtggcaa tgggcatgct
    14521 aatcccattt gtttggatgg tgttgacgtc gtttaaaacg gtaacagaat ccactcagat
    14581 gaatcctttc caatttttac caagtaactg gatggtgagc aattatacag aggctattcg
    14641 gactaataat ttcccgatac tttatctgaa cactattttg atgatgttat ggcgtatttt
    14701 tagctcggtc atgttctcag ccatggcagc ttatgccttt gctcgattgg agtttcctgg
    14761 gcggaatttc ttgtttggct tggtcctctt tcaaatgatg gtgccacctc agttattcgt
    14821 cattcctcag tatttgatga ttgaccaact tggtatgcgc aatacgattt ttgctctggt
    14881 attcccagga attgtcagtg cctttgggac attcttgctc cgtcaatttt tcatgggtct
    14941 tccaaaagaa ttggaggaat ctgcaaaatt ggatggttgt aatatcgggc agaccttctt
    15001 taaggtcatg ttgcctttgg ccaagtctgg cttgattgcc ttagcgattt ttacggcttt
    15061 gtttgctttc aaggatttgc tgtggccatt gattatcaac tcagaagcgg acaaggctac
    15121 cctgtctagc gcactttcta agattcaggg agcttacgct gttaactatc ctcagctcat
    15181 ggctgcaagt gttttggcta tttggccgat gttggtactg tatgtgatat tccagaagca
    15241 gttcatccaa gggattgcga catctggtgg gaaattatag gaggctacaa tgggaattat
    15301 ctttcatgaa aagacaaagg aattccatct ttacaatgag tttattagtt atgtgttgac
    15361 ggtcttgcct aatggtcata ttggtagttt gtattatgga aagcgtgtaa cagaaacagc
    15421 ttcttatcag tatgtgcgtg aagatgagta tcgtgctttg acttcctttg tagaagacga
    15481 tgaccgtttc ttttcgctcc aatatgccaa tcctgaattt gcttgttatg gtacaacgga
    15541 ctatttttct ccgacttttg agttggtgca gaaggacggt agtagcctgt cgcactttgt
    15601 gtatcaaggt catgtgattt atgctgggcg atcggctttg aagggtcttc ctcatctcta
    15661 tctggatgag gaggggcagg cagatagttt ggatatttat ttgttggatg aaaagtcgca
    15721 gacccgcctg gtcttgtctt atacgatttt tcgagattat ccagcgatga cccgctctgc
    15781 tcgttttgaa caactcggag aaaaatctgt ctgtttgaac cgtgccttga gtatgacact
    15841 ttgtctgcct gatatggact atgactggtt acatttggat ggggcttggg ggcgtgagcg
    15901 acatctgcaa ctatcgccgc ttcatcaagg ttgtcagtct atttacagtc tcaaaggggc
    15961 tagtagtgcg gagcacaatc ccttcatggc tttgaaacgc ccaactgcgg atgagcaaca
    16021 gggagaggta ctgggcttct cgctcgtcta tagcggaaac tttttggcgc aggtggatgt
    16081 gagttctttc caaaaaacaa gagtgagtat gggaatccat cctgagcgtt tttattggac
    16141 attagataag ggagaagagt tccagacgcc tgaggttgtt atggtttata gcgagcatgg
    16201 tttgaatggc atgagccaaa cctatcaccg cctcttccag aaacatttgg tgcgtggcta
    16261 ttggcgtgat agagaaaggc ctgttctctt gaataattgg gaagccatga gttttgattt
    16321 tgatgaggaa acaattcttt ccttggctaa agaggctgca ggtcttggtg ttgaactctt
    16381 tgtcatggat gatggttggt ttggcaagcg aaaccatgac cgagcaggct tgggagattg
    16441 gacggtcaac cgagagaaat tgccaagtgg cttgacaggg attattgacc aagttcatgc
    16501 tatgggaatg aaatttggac tttggatcga acctgaaatg gtcaataggg acagcgatct
    16561 ataccgagcg catccagact ggattttcca tcatcctcag catagtcaat cgcacggacg
    16621 ccaccaatat actttggatt tgtcgaggga ggatgtttat caaaatattc atgaccaact
    16681 ccattggctg ttggctgagc atgatattga ttatatcaag tgggacatga atcgctatat
    16741 gacagaggtc tttacatgta cacgggaggc ggatcgacaa ggtgagacct tccatcgcta
    16801 tattctcaat ctctaccgtt tatatgatag cctgatagca gcttttccga cagtcttatt
    16861 cgaatcttgt tcaagtggtg gagctagatt tgatccaggg atgctctact atgcaccgca
    16921 aacctgggtc agcgatgaca cggacgccat ggaacgcttg aaaatccaat atggaacgag
    16981 catagtctat cctttgaata gtatgggttg ccatgtgtca gctagtccaa atcagcagtt
    17041 ggggcgtgct actccactaa acacacgtgc caatgttgca ttctttggaa gttttggcta
    17101 tgaattggat ttgtttgagt gtagccaaga agagttggaa gagatgaaag agcagattgc
    17161 cttttacaag actcaccgta agattttcca acagggtatt ttcactcgtc tgaaaagtcc
    17221 attcgatgga gaaataacgg cttggcaggt ccaatcctcg gatggtcagc aagtcatcgt
    17281 gggttattac cgtcggttaa caactgccaa tcttgcctat caacgcttgt acttgcaggg
    17341 gttggaagag gaagcggtct atcaccttga tggagaagtt tatactggta gccaattaat
    17401 gcatacaggt ttgtcgattc gacatgggga ccatgttgga gacaataagg actttacttc
    17461 ttgtttgatg gtccttcaga aacaacagta gcgtccctat tcctaggccc agctatcggc
    17521 tgggtctatt ttatgcagaa agtacatcaa taagggcaag acctctactt atactcttcg
    17581 aaaatcaaaa ttatccgttg tcaacttgcc ttgatgaact ccagttctat cttcggcttc
    17641 gtttcctagg ctacttttga ttttcattga gtattagata taagaattct tgaaaaacgg
    17701 gttgcaatgt gagaaaatga gagaataatt cttcaatcaa ttagtgagag gaagggagat
    17761 aatgaatgaa gaaatatgct tttcgtgaag ggatgcgcga tgccattccc actgtattgg
    17821 gctatgccag tattggtcta gcctgcggag tcgtatcggt taattcgggc atttctactg
    17881 tggagatggg gctgatgagc ctattaatct atgccgggag tgctcaattt gtcatgtgtg
    17941 ccatgatttt tgcaggtgca cctttgttat ccattgcagt aaccgttttc tttgtcaacc
    18001 tgcgaaattt tctgatgtgc ttgcatgcga caactatttt tcaaggaaat cgtttagggt
    18061 cgaatatctt gataggatcc tttgtgacgg atgaatccta tgcagttctt ttgcgtaagc
    18121 agattgagca tcacaccatt actccagaat ggatgtacgg aaataatttt gcaagctacg
    18181 cctcttgggt tacattcacg actctcggca atctgattgg tggtctcatt ccaaatcctg
    18241 agcagtttgg attggacttt gccttggttg ccatgtttgt cggtattttt gcaggtcagt
    18301 tagaggctat ggttcggacc attccgctga aaaaaattgg cttaattctg ctggcggtgg
    18361 gtctgggcta tatcggtctg tctatgatgg tatcgtccta tgtggcggtg ctcttggcga
    18421 cactattagg ttgctttgtg ggggtgatga ttgatgataa aaactaacat tttactgatt
    18481 attctaatga cggtcctagt gacttgggtg ccacgtgtcc taccttttgt actgacgcaa
    18541 aataaatctc tccctccaaa gttggtgaaa ttcctcagct ttttgccaat cactatcatt
    18601 tttacattga ccctctccag tattatggat gagaaagttg gctccttgcc aagttttctc
    18661 cctgtggaaa gcttagctat tcttccgaca ttttttgtgg ttttgaaaac aaaaaatatc
    18721 cttttagcag tcttggttgg tgttgttaca accgcagtgt tacgtttgat attttaggag
    18781 gtttttatgg taaaagaaaa tgccaaaggt gtcggacttt gtttcctttt ggctttggtg
    18841 ggtcagtggt taggtcattt atttccactt gtgggtgggc ctgtttttgc ccttctaatc
    18901 gggatgagtc tgtatcccta tttttcaagt aaaaatgcat ttcattcagg cttaaccttt
    18961 acttccaaga aaattttaca gtatgctgtg atttgtttgg gatttggatt aaacttatca
    19021 gcggtcttgg cagtgggtcg tcagtctctt ccaattattc tttcaaccat cagtttcgct
    19081 ttatttttgg catttttgat gtggaaatgg ttgcctattt catctcatct ggcgaccttg
    19141 attggcgttg ggacctctat ctgtggaggg tcagccattg cggcgacagc tcccattatt
    19201 caggcggatg atgaggatgt ggcacaagct atctcggtca tctttctctt taatgtcttg
    19261 gcagccttgg tctttcctac tttggcaact tggcttgggg tttcgactga ttcgggtcac
    19321 gcctttggga tgttcgccgg aacggcggtc aatgacacct cctctgtgac agcagcggct
    19381 tcgacttggg atagcttata tggtctaggc agtcagacct tagatacagc agtgacggtt
    19441 aagctaacac gaactttagc cattatcccg attacaacgg ttttagcaat ttggcaaacc
    19501 cgaggaaagg gtctcaaggc agacaagaag tccctcctga caggttttcc gacctttatc
    19561 ttgtacttta tcttagctag tctaatcaca acgatagcgg gtggttttgg tataggggca
    19621 gattggtttg ctccgttgaa gtccctctct aaatttttca tttgtatggc tatggtggcg
    19681 attggtttgc ggaccaatgt gtttgccttg gtgaaaaatg gcagagcggc tctcctggtt
    19741 ggactagttt gctggctggg agtgactgtg ttgacactgg tctggcaagc gattttgggg
    19801 atctggtaag agaaatgtga gtgggaaaga catcatttcc tatttcaaac aatttttgag
    19861 aagtaaatcg cagtgccgca gggctttttg tcgctcggtt tttagtaccg ttgaacaact
    19921 taaaactagt tagttttgtt taaaatgtaa ttgaattgcc tttttaagta ggctgtttac
    19981 acgatatttg tcttccttta tataaa
//
LOCUS       DATUCG010000029        19878 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.030, whole genome shotgun sequence.
ACCESSION   DATUCG010000029 DATUCG010000000
VERSION     DATUCG010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19878
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.030"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(392..1201)
                     /gene="yidA"
                     /locus_tag="VCA20_000833"
     CDS             complement(392..1201)
                     /gene="yidA"
                     /locus_tag="VCA20_000833"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEP1834682.1"
                     /translation="MSIKLVAIDIDGTLLNSQHQITAEVHSAIQEAKKAGVKIVIATG
                     RPISGVQAILEELNLTEAGDYVITFNGGLVQDAATGEDIVKDTLTYEDYLDIESVARK
                     LRLPMHASTKEGIYTANRNIGKYTIYESTLVSAPIFYRTPEEMADKEIIKTMMVDEPE
                     ILDAAIPLLPTSLTEKYNVAKSAPFYLEITPKTVNKGQAIIHLAEKLGLTMDQTMAIG
                     DQENDRPMLEVVGNPVVMENGNPELKKIAKYITKSNDESGVAHALREWVLK"
     gene            complement(1191..2504)
                     /locus_tag="VCA20_000834"
     CDS             complement(1191..2504)
                     /locus_tag="VCA20_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEP1834683.1"
                     /translation="MIEKVFRDPVHNYVHVDHELIYKLINTKEFQRLRRIKQLGTTSY
                     TFHGGEHSRFSHCLGAYEIARRITQKFEDKYPQIWDTNESLLTMVAALLHDVGHGAYS
                     HTFERLFDTDHEEMTCAIITSPETEINTLLKQVSPEFPDKVASVIKHTYPNKQVVQLI
                     SSQIDVDRMDYLLRDSYFTGANYGEFDLTRILRVIRPTENGIAFKETGMHAVEDYVLS
                     RYQMYMQVYFHPASRSMEVLLQNLLNRAKLLYTSEQEFFARTSPRLLPFFEHRIRLTD
                     YLSLDDGVMNTYFQSWIDGPDRILSDLAQRYINRKVLKSITFQAEEEETLDRLRSLVA
                     DVGFDPEYYTAIHHNFDLPYDIYRPNAEKKRTQIEIYRKDETLVELSSLSPIVHSLSG
                     TIHGDSRFYFPKEMLEETGIFAPQISDFNSHIHNDHFIIGESNEH"
     gene            2603..3490
                     /locus_tag="VCA20_000835"
     CDS             2603..3490
                     /locus_tag="VCA20_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEP1834684.1"
                     /translation="MNNQIILLTIQVFIVAQILWMLYTILSYAKKHDISLKEKFWTGF
                     GIGYVTDLLDTLGIGTFATTTTMFKLTKLVEGDRKIPATMTTAHIIPVLVEALLFITI
                     VEVDMVTLIAMAAAAFTGAFVGAKVTQKWDTKKVQRILGILLIIAACFMVYRMITNPG
                     ADLTNEVKGLTGWKLAVGIAFDFMVGMLMSMGLGNYAPELIFFSLMGISPAVALPVMM
                     LNAAMIMTAGAKQFIQSGRVNWPGVPGIIVGGVLGVLIAAFFLSNLDINNLKILAVFI
                     AAYTGFTLLRSSFVTRIKK"
     gene            3502..3888
                     /locus_tag="VCA20_000836"
     CDS             3502..3888
                     /locus_tag="VCA20_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEP1834685.1"
                     /translation="MDIHLRNEIDLDGQIEVVDQQFQVEVKEKDGNLYLIYSNDEAEK
                     VVIKCDEEELVMTRFSTPKSIMRFISGKEAIVTIPTPLGIQHFVTDTKRYQLNRSAQS
                     VQLQYELKGLENQQLFASYDLEISWK"
     gene            4363..7050
                     /locus_tag="VCA20_000837"
     CDS             4363..7050
                     /locus_tag="VCA20_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEP1834686.1"
                     /translation="MSKEQKRDLFYTQSEEQVLSSMGSSLDGLSSDEASKRLADYGRN
                     ELDEGEKRTLLAKFLDQFKDLMIIILIAAAILTVITEGSHGLTDAIIILAVVILNAAF
                     GVYQEGQAEAAIEALKNMSSPVASALRDNHVVEVDSKDLVPGDIVLLEAGDVVPADMR
                     LLEANSLKIEEAALTGESVPVDKDLSAVLAEDAPIGDRVNMAYQNSNVTYGRGLGVVT
                     NTGMYTEVGHIAGMLANADEIDTPLKQNLHQLSKVLTYAVLVIAAVTMAVSVFVRGEG
                     ILPALMTSVALAVAAIPEGLPAIVTVVLSLGTQVLAKRNSIIRKLPAVETLGSTEIIA
                     SDKTGTLTMNQMTVEKVYTNGQLVDAKEALDASNTTLRVMNFANDTKVDPTGKLIGDP
                     TETALVQFGLDQNFDVREVLVSEPRVAELPFDSTRKLMSTVHQQAAGNFFVAVKGAPD
                     QLLKRVTQIEENGTIRPITDADKQAILATNKDLAKQALRVLMMAYKYVNAIPELESDV
                     VENDLVFSGLVGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGIIDP
                     NDTEDHVFTGAELNELTDEEFQKVFKQYSVYARVSPEHKVRIVKAWQNDGKVVAMTGD
                     GVNDAPSLKTADIGIGMGITGTEVSKGASDMVLADDNFATIIVAVEEGRKVFSNIQKT
                     IQYLLSANTAEVLCIFLATLFGWDVLEPVHLLWINLVTDTLPAIALGVEPAEPGVMQH
                     KPRGRNSSFFSGGVLSSIIYQGILQTALVLGVYGFALLYPEHAGDNHAIHADALTMAY
                     VTLGLIQLVHAFNVKSVYQSIFTVGPFKNKLFNWSIVAAFLLLMATVVIPGFNTFFKV
                     SILTPTQWMVTVIGSGLMVVVVEIVKFVQRKMGLDEKAI"
     gene            complement(7076..7465)
                     /locus_tag="VCA20_000838"
     CDS             complement(7076..7465)
                     /locus_tag="VCA20_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834687.1"
                     /translation="MRVIIHYPKPIKQLSSQKLPLLVDGKIAGTVTQGKILSLPITQP
                     SVTLSIRGDKKSSIQVKEGDRVQIVENSKYFILYYFSLAIVPIALLFNLPTLVKTILL
                     ILALAITLLGQEIFPKYIISTEKSSDH"
     gene            7599..8015
                     /locus_tag="VCA20_000839"
     CDS             7599..8015
                     /locus_tag="VCA20_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HEP1834688.1"
                     /translation="MNYLQNYILSKSSYLPSDKLFILQKELEDLDDEALNVLMMVEMR
                     QPLVALILAIFFGEFGVDRFYVGNKELGFAKLIAFAVSFVTLFILIGFLLFLGLYLWK
                     FIDCFLIMRACKEANFERLMLQIHQYKAFQHSNQTF"
     gene            8183..8671
                     /locus_tag="VCA20_000840"
     CDS             8183..8671
                     /locus_tag="VCA20_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEP1834689.1"
                     /translation="MQTKKMSMFLFFAYLLLLTWMIVFKMDLSIVYGRYGYASINLIP
                     FAGTAVYDGVLDFPEILFNIVSFIPFGIYMEMLFRKASWVANLYLIMLVSLCFEVLQY
                     LLLLGVADITDLLANGLGGAIGINIMYVLTSIWREKAYVRMNIFCFVLTFFIILITYL
                     AM"
     gene            8712..9839
                     /gene="queG"
                     /locus_tag="VCA20_000841"
     CDS             8712..9839
                     /gene="queG"
                     /locus_tag="VCA20_000841"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEP1834690.1"
                     /translation="MNIKEEIIKLSQEIGISKIGFTTADDFAYLEKSLRASQEEGRSS
                     GFEHKNIEERIRPKLSLESAKTIISIAVAYPRHLPVKPQKTQYKRGKITPSSWGLDYH
                     YILQDKMERLARGIEKLTDGLEYKAMVDTGALVDTAVARRAGIGFIGKNGLVISKEFG
                     SYMFLGELVTNLDIEPDQPVDYDCGDCNRCVAACPTSCLLGDTTMNARRCLSFQTQDK
                     GMMDLEFRKKIKAVIYGCDICQICCPYNKGISSPPVVEIDPELAEPELIPFLDLSNGQ
                     FKEKFGLIAGSWRGKNILQRNAIIALANSNDRSAIPKLLEIIDKKQNPVHMATAIWAL
                     GQLVKQPNDEMVSFIAGISSDNDDVRAEQTAFLNMVKDLQM"
     gene            9874..10969
                     /gene="prfB"
                     /locus_tag="VCA20_000842"
     CDS             join(9874..9945,9947..10969)
                     /gene="prfB"
                     /locus_tag="VCA20_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775232.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEP1834691.1"
                     /translation="MDTAEIRQKVEELGKKLTSFRGSLDLEGLEEEIAILENKMTEPD
                     FWNDNLAAQKTSQELNELKNTYGNFHQMLDLYDESEILLDFLAEDESVRDELVEKLAE
                     LDKTMTAYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGEMLLRMYQRYGNAKGFTV
                     ETLDYQAGDEAGIKSVTLSFTGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFTSVE
                     VMPELDDTIEIEIRDDEVKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVTQSTVDRT
                     QYGNRDRAMKLLQAKLYQLEQEKKAAEVDSLKGDKKEITWGSQIRSYVFTPYTMVKDH
                     RTGYEVAQVDKVMDGDLDGFIDAYLKWRIS"
     gene            11111..11803
                     /gene="ftsE"
                     /locus_tag="VCA20_000843"
     CDS             11111..11803
                     /gene="ftsE"
                     /locus_tag="VCA20_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEP1834692.1"
                     /translation="MGIIEMKDVSKKYGNGTTALRGVSVNVEAGEFTYIVGPSGAGKS
                     TFIKLLYREEKLDKGSLKVGKFDLAKIKKRDVPLLRRSVGVVFQDYKLLPKKTVFENI
                     AYAMEVIGEKPRNIKKRVMEVLDLVGLKHKIRSFPNELSGGEQQRIAIARAIVNNPKV
                     LIADEPTGNLDPENSWEIMNLLERINLQGTTVLMATHNSQIVNTLRHRVIAIEDGRVV
                     RDEEQGEYGYDD"
     gene            11796..12725
                     /locus_tag="VCA20_000844"
     CDS             11796..12725
                     /locus_tag="VCA20_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP1834693.1"
                     /translation="MIRRFFRHFIESLKSLKRNGWMTIAAISSVAITLTLVGLFASVI
                     LNTAKLASDLEQNVRINVYLRANSTDQAETIVNEAGETVANPDYQKVYNQITAIENVQ
                     SVTYSSKDEQLQKLTATLGDTWNLFQGDANPLYDAYIIDTTEPQDVKTVAAEIAKIDG
                     VTEVRDGEVETERIFKLANLVRTWGLAATGLLLFTAVFLISNTIRITIISRSREIQIM
                     RLVGAKNSYIRGPFLWEGAWVGLLGAILPSALVYSLYKMIYTSVNASLASQDLSLISM
                     NVFVPGMIGALFVIGIIIGSLGSVISMNRYLKI"
     gene            complement(12778..12930)
                     /locus_tag="VCA20_000845"
     CDS             complement(12778..12930)
                     /locus_tag="VCA20_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HEP1834694.1"
                     /translation="MPTLILFLIIVILFSLAIRSLIKGKGSCGDCSCDCAIKQEMNES
                     SRHRHP"
     gene            complement(12930..15074)
                     /gene="feoB"
                     /locus_tag="VCA20_000846"
     CDS             complement(12930..15074)
                     /gene="feoB"
                     /locus_tag="VCA20_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HEP1834695.1"
                     /translation="MTKHIALAGNPNSGKTTLFNLLTGTNQRVGNWPGVTVERKSGTI
                     KKRKHLLIQDLPGIYSLSPYTPEERVARDYLIADQPAAILNVLDATNLERNLYLTLQL
                     LEMGLPIVIALNMTDALKSQGRSINSDQLSYQLGVPVQPISALKKLGISQLLHEVEKI
                     TNQQAEPIYPQYDKQFEAGLAQVIDLLSDSVPNHQKRFYAIKLLEQDQVILDQLQLNA
                     QDMLDLTEIIAILEKIYADDMEAIIVNQRYQFIEKITELVTKDSDKTFNLSDNIDRLF
                     TNRFLALPIFAAVMWLTYFLSIQTVGTMGTDWVNDVLFGELVPGLIQENLDRFEIAGW
                     LQSLVLDGIIAGCGAILGFVPQIFVLFVCLGILEDIGYMSRVAFVMDRIFRRFGLSGK
                     SFIPMLISTGCGVPGVMASRTIENEQDRKITIMTATFMPCSAKLPIISLVVGAFFPDN
                     PWIAPSAYFVGMAAIVLSGMALKKTKQLGGVASPFIMELPSYHLPKLSTVLRYAFDKA
                     LSFIKRAGTIIFVTNIIIWFSSSYNWSLQMVETDESILASIGRSFSLLFAPLGFGNWR
                     ATVAAITGLLAKETVIATFGILYKLGETTEENPELWGLLQQDYTALSAYSFLVFNLLC
                     APCFAAIGAIHREMGEAKWTWIAIGFQTGLAYASSLVIYQIGLVLIYGQLPTVWTFIA
                     IFLIITAIHSLVKKPTNTLPIVTLKTLEKGEY"
     gene            complement(15071..15541)
                     /locus_tag="VCA20_000847"
     CDS             complement(15071..15541)
                     /locus_tag="VCA20_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HEP1834696.1"
                     /translation="MKLQNLQVGQSYLVKDCLTQDDIRKHLAHLGLKVGEEIRIISKT
                     KTNAIFKVKASRLALDREIIESLVLIEKSATEIINLSEAPIGSSAKVMDIYATGALRR
                     RLMDMGLTKNTQLFLKKVAPLGDPIEITLRGYELTLRKSEAQMIGVQITSEVRK"
     gene            15698..15770
                     /locus_tag="VCA20_000848"
     tRNA            15698..15770
                     /locus_tag="VCA20_000848"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15731..15733,aa:Thr,seq:ggt)
     gene            16340..18067
                     /locus_tag="VCA20_000849"
     CDS             16340..18067
                     /locus_tag="VCA20_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1834697.1"
                     /translation="MFRLIFDYIKRHKWLYLLVAVTLIIYDATLLLPTQIIQRMVDIL
                     TKNELTQAILVQEMTLLLLVTVLNYATAFIWHLKLFQASVNFKFDMQQRAFKKLVTMR
                     TPFYEKFRSGDVMTRFSTDVDGLMEMVGYGLMIVVYAGGMLAFIIPTMFFIDWKISLV
                     ALLPMLFMTLCIFFIGRKQDEAIDANREAVAQLNNEVLEVIEGIRVTRAYSKKANQKA
                     QFQARTKQLAQGGDRITSLQSLYNPLATVCLGLSTIFVLLMGAQAVKAGQLTLGQVIA
                     LQLYVGSLLEPFWTLADFILVYQTGKTSFEKLQELIETGDDLEADGSKEIAELSSISF
                     KNYSFSYPQAERASLQDINWTLKAGQTVGIVGKTGSGKTTLVRQFLRQYPIGRGNFFI
                     NHQSVLDFKRSSIEEKIGYVPQEHILFSRSVGENIALGKVASSSEEIEQAIATAAFSQ
                     DLKRMTDGLDTMIGERGVSISGGQKQRISIARAFLREPDLLILDDSLSAVDARTERQI
                     IQNIQKERAGKTNVIVTHRLSAVNHADWVLVLDEGRIVEKGRPADLLAQRGWYYEQYQ
                     RQQSQEGGE"
     gene            18067..19806
                     /locus_tag="VCA20_000850"
     CDS             18067..19806
                     /locus_tag="VCA20_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1834698.1"
                     /translation="MTILGFLLKQIGRVKWLFIVAVGFYLLASTMVRLAPLLIQQAID
                     GPITDLSKGLPFDEAVFLNQSAQYMGMIILGAIGFYLSMRLLMHCANRIAENLRNQAY
                     DVMQRLPISYFDDKPAGKIATRIVNDTETLRTQFYGTLVNAFNNIVRLLFTYGVLFYM
                     NRSLGWLMLLLIPLYIGIQFAYKKMTDKPMKDFYDARSDVNTQVNETMNGASLIQLFG
                     QEERIMKEFEVTADKMRRADNKIIWAQSLATWNLSGFLQNLVIAAILTVVGYQFLAGQ
                     DGVTAGKLFVYVNYIEGVFIALGALVQQFPNMLRSFETGKRLMALLEEEVEDDCDRIL
                     EVEQGQVVFEHVNFSYEEHRPVLKDITIRAEKGETVALVGHTGSGKSSIMNLLYRFYD
                     PQEGRVLIDEKNIRDYSRESLRSHMGIVLQDPYLFTGTIASNVSMNEEEADRTRIMQA
                     LEKVGAGPMLSRLEKGIDEPVVEKGAAFSSGERQLIAFARTLYSDPKILILDEATSHI
                     DTETEEIIQHAMEVVKEGRTTFIIAHRLSTIQNADQILVLDQGRIIEHGKHEELVARG
                     GVYAQMHEIQAKV"
BASE COUNT         5609 a         3587 c         4569 g         6113 t
ORIGIN      
        1 ggtaaccaat ccacgaatat cagagtggcc aatgcccgaa gctaagaact aggctctttc
       61 tagatacttt tcatttttta atcagttcga ttgtttttac ttgacaaagg gctttacata
      121 tcagtggttt agtttttctt ttattacttc gacgtaagag gaaactccgt ttccttattt
      181 ccaacttcaa acactccact ggattgtttg aactcgcttt gctgtactct gcaggggtga
      241 cttgctccgc aattgttatc tccaaccttg cacagtcact cgactgtaca agctgtctgc
      301 agctcgttgc ctagtactaa aagtaaacta aaagactata acaaaaagca tcggattttg
      361 aattcgatgc tttttttgat attttaagag gctatttcag tacccattct ctaagagcgt
      421 gggctacacc gctctcatca tttgacttcg taatgtattt ggcgattttc ttcaattcag
      481 gatttccgtt ttccatgaca acaggattac caacaacttc caacattgga cggtcatttt
      541 cttggtcacc gatggccatg gtttgatcca ttgttagtcc aagcttctca gctagatgaa
      601 tgatagcttg gcccttgtta acagttttag gggtgatttc caaataaaat ggtgctgatt
      661 ttgctacgtt gtacttctca gtcaagctgg tcggtagaag tggaatagca gcatcaagaa
      721 tttctggctc atcaaccatc attgttttga tgatttcctt gtcagccatt tcttcaggag
      781 tgcgatagaa aatgggtgca cttactagtg tggattcgta aatggtgtat ttaccgatgt
      841 tacggttggc agtatagata ccttccttgg tgctggcatg cattggaagt ctgagtttac
      901 gggcaacaga ttcgatgtcg agataatcct cataagtcaa ggtatctttg acaatatctt
      961 cacctgttgc tgcatcttgg actaagccac cgttaaaagt gatgacgtag tcacctgctt
     1021 ctgtaagatt gagttcttct aaaattgctt gaacaccgga aatggggcga ccagtagcaa
     1081 taacaatttt aacgcctgct ttttttgctt cttggattgc tgagtgtact tcagctgtaa
     1141 tttggtgttg actatttagt agtgtaccat caatatcgat ggcgacaagt ttaatgctca
     1201 tttgattctc caattataaa gtgatcgtta tgtatgtggc tgttaaaatc agatatttga
     1261 ggtgcgaaaa ttccagtttc ttctaacatt tcttttggga aatagaagcg gctatcgcca
     1321 tggattgtgc ctgataagga atggacaatt ggtgataggg aggatagttc gaccaaagtt
     1381 tcatctttgc gataaatctc aatttgagtc cgtttctttt ctgcattcgg tcggtaaata
     1441 tcatatggca aatcaaagtt gtggtgaata gcagtatagt attctgggtc gaatccgacg
     1501 tctgcgacca ggctacgcag gcgatccaaa gtttcctctt cttcggcctg gaaagtgatg
     1561 gatttgagca ctttcctatt gatatagcgc tgggctaagt cagataatat acgatctggt
     1621 ccatctatcc atgattgaaa gtaagtattc ataactccat catcaagact gaggtagtcg
     1681 gttagacgga tacgatgctc aaaaaaaggt aatagccgag gtgaagttcg tgcaaaaaac
     1741 tcctgttcac tagtatatag tagtttggca cgattgagta aattctgcaa taggacttcc
     1801 atagagcggc ttgctggatg aaagtagact tgcatgtaca tctggtagcg gctgagaacg
     1861 tagtcttcta ctgcatgcat accagtttct ttgaaggcaa tcccattttc tgtcgggcga
     1921 atgacacgta atatcctagt taggtcaaat tcaccatagt tcgcacctgt gaagtaggaa
     1981 tctcgtaata gataatccat ccggtcaaca tcaatctggc tcgaaatgag ttgaacgacc
     2041 tgtttatttg gataggtgtg tttgataaca ctagcaacct tgtctggaaa ctcaggtgaa
     2101 acttgtttca acagagtatt gatttccgtt tctggactgg taataatagc acaggtcatt
     2161 tcctcgtggt ctgtatcaaa tagacgctcg aaggtatgag aataggctcc gtgtccgaca
     2221 tcatggagta gcgcagcaac catggtcagt agggactcat ttgtatccca gatttgtgga
     2281 tacttgtctt caaatttttg agtaatccgt ctggcgattt cgtaggcgcc aaggcagtgc
     2341 gaaaagcggc tatgctcgcc accatggaag gtataggaag tagtccctag ttgcttgatg
     2401 cggcgtagtc gctgaaattc ttttgtattg ataagtttat aaatcagctc atgatcaaca
     2461 tgtacatagt tatgaactgg atcgcgaaat actttttcta tcatagtttc attataacaa
     2521 aaaagggaga taaattcctg atttttctaa aacacaagga tattgctatg tacgcaatgg
     2581 aatcttttgg tataatgaag aaatgaataa tcaaattatc ttattaacta ttcaggtctt
     2641 tattgtagcc cagatacttt ggatgcttta cacaattcta tcctatgcta aaaagcatga
     2701 tatttctcta aaagagaaat tttggacagg gtttggtatc ggttatgtta ctgatttgct
     2761 agacaccttg gggattggaa cctttgcaac gacaaccact atgtttaaac tgacaaagtt
     2821 ggtggaaggc gatcgtaaaa ttcctgcgac catgacaacg gcgcacatca taccagtctt
     2881 agtagaggcg ctattgttta ttacgatagt tgaggttgat atggtcactc tgattgctat
     2941 ggcagcagcg gcatttacgg gtgcctttgt tggtgccaag gtaactcaaa aatgggatac
     3001 aaagaaagtt caaagaattc ttgggatttt actaattatt gcagcctgtt tcatggtcta
     3061 tcgcatgatt actaaccctg gcgcagatct tactaatgaa gtaaagggct taactggttg
     3121 gaaactggct gttggtattg cctttgattt tatggtggga atgctgatga gtatgggact
     3181 gggtaactat gctcctgagt taattttctt ctcattgatg ggaattagtc cagcggttgc
     3241 tcttccagtt atgatgttga atgctgctat gattatgaca gctggtgcca agcagtttat
     3301 ccaatcagga cgagtaaact ggccaggagt tccaggaatc attgtcggtg gggtgctagg
     3361 tgttttgata gccgctttct tcctatcaaa tttggatatt aataatttga aaattttagc
     3421 tgtcttcatt gcagcgtata caggctttac tctgcttcgt tcatcctttg ttactaggat
     3481 taaaaaatag taggaggaac tatggatatt catttacgaa atgaaattga tctggacggg
     3541 cagattgagg tagtggatca gcaatttcaa gtggaagtta aggaaaaaga tgggaatctc
     3601 tatctcattt atagtaacga tgaagctgaa aaagttgtca taaaatgtga cgaagaagag
     3661 ttagtgatga cacgcttttc cactccgaag tctattatgc gattcatttc tggtaaagaa
     3721 gctattgtga ccattcctac accgttagga atccagcatt ttgtgaccga tacaaaacgt
     3781 taccaattaa accgttcggc tcaaagtgtc cagttgcaat atgaattaaa gggattggag
     3841 aaccagcagc tttttgcttc ctatgatttg gaaatctcgt ggaaataatc tcttatttcc
     3901 agaaaaatat tcttgaattt ctcgggattt ttgatataat agttaacaaa tcgagggagt
     3961 agctagcgga aaattccgtg atattgtcgt caatccgact tgtttccggc aatatattaa
     4021 atagcgagac ttgttttaga acctgtcttc gcaagtaagg tatttttaga taagagatag
     4081 ttttttgtct attaaggcag tttttgaagg aatactgact gtatttcaaa aaaaactaat
     4141 attcaatgaa aatcaaagtc aggctaggcg acgccgatgc agattgaact aaagttcatc
     4201 aattcaagtc aacaacgcct gagtttgatt ttcgaagagt ataaagatga gtgacagaaa
     4261 actagcccta gatagaagtg ccgagctgtg aagagaggtt ctcattaaac aggtctcttt
     4321 tttatttgtt aggtaaaaaa gggaccgaga aggagaagga aattgtcaaa agaacaaaaa
     4381 cgcgatttgt tttatacaca gagcgaagag caagtattgt caagcatggg gtcttctttg
     4441 gacggtttgt ctagtgatga ggcaagcaaa cgtctagcag actacggtcg taacgaattg
     4501 gatgagggtg aaaaacgcac actcttagct aaattcttgg atcagttcaa agacttgatg
     4561 attatcattt tgattgcagc agcaatcttg acggtgatta cagaaggttc acacggtttg
     4621 acagatgcca ttatcatctt ggccgtggtt atcttgaacg ctgcctttgg tgtttatcaa
     4681 gagggtcaag ctgaggcagc tattgaggcg ctaaaaaaca tgtcaagccc agttgcgagt
     4741 gcgcttcgtg acaatcatgt agtcgaagtt gattctaaag acttggtacc aggtgacatc
     4801 gtcttgcttg aagcaggtga tgttgtccct gccgatatgc gtttgttgga agctaactcc
     4861 ctcaaaatcg aagaagcagc tcttacaggt gagtctgtac cagttgataa agatttgtca
     4921 gcagttttgg ctgaagatgc accgatcggt gaccgtgtca acatggctta ccaaaactca
     4981 aatgttactt acggacgtgg tcttggtgtg gttacaaata caggtatgta cacggaagtt
     5041 ggtcatattg ctggtatgct tgctaatgct gatgagattg atacaccatt gaaacaaaac
     5101 ttgcaccagc tatcaaaagt cttgacatat gcagttcttg taattgcagc ggtaacgatg
     5161 gctgtttcag tcttcgtacg tggtgaaggt atcttgcctg ctcttatgac ttctgtagct
     5221 cttgcggttg cggccattcc agaaggtttg ccagctattg taacggttgt cctttcactt
     5281 ggtactcaag tattggctaa gcgtaattcc atcatccgta agttgcctgc tgttgaaact
     5341 cttggttcaa cagaaatcat cgcttcagat aaaactggta ctttgactat gaaccagatg
     5401 actgttgaaa aagtctatac aaacggtcaa ttggtggatg ctaaagaagc tttggatgca
     5461 agcaatacaa cgcttcgtgt tatgaacttt gccaacgata caaaagttga cccaactggt
     5521 aaattgatcg gtgacccaac tgaaacggca cttgttcaat ttggtttgga ccaaaacttt
     5581 gacgttcgtg aagtcttggt ttctgagcct cgtgtggctg aattgccatt tgactcaaca
     5641 cgtaaattga tgtcaactgt tcaccaacaa gctgctggaa acttctttgt agctgttaaa
     5701 ggtgccccag accaattgct caaacgtgta acgcaaattg aagaaaacgg tacaatccgt
     5761 ccgattaccg atgcggataa acaagccatt cttgccacta acaaggactt ggctaaacaa
     5821 gcccttcgtg tcttgatgat ggcatacaag tatgttaatg ctattcctga attggaatct
     5881 gacgttgttg aaaacgacct tgtcttctca ggtttggttg gtatgattga ccctgagcgt
     5941 cctgaagcgg cagaagctgt tcgtgttgct aaggaagctg gtatccgtcc aatcatgatt
     6001 acgggtgacc accaagatac agctgaggcg attgccaagc gtcttggtat catcgatcca
     6061 aacgatacag aagaccatgt atttacaggt gcagagctca acgaattgac ggatgaagag
     6121 ttccaaaaag tcttcaaaca atactcagta tacgctcgtg tatctccaga acataaggtt
     6181 cgtatcgtta aggcttggca aaatgatggt aaggttgttg ccatgactgg tgacggtgtt
     6241 aacgatgcgc catcacttaa gacagccgac atcggtatcg gtatgggtat cacaggtacg
     6301 gaagtttcga aaggtgcctc tgatatggtc cttgcagatg acaactttgc aactatcatt
     6361 gtggcggttg aagaaggacg taaagtcttc tcaaacatcc aaaaaaccat ccagtatctc
     6421 ttgtcagcta acactgctga ggtattgtgt atcttccttg ctaccctctt tggttgggac
     6481 gtattggagc cggttcatct tctttggatc aatttggtaa cagatacact tccagcgatt
     6541 gcccttggtg ttgaacctgc tgaaccaggt gttatgcagc acaaacctcg tggtcgtaac
     6601 tcaagcttct tctcaggtgg tgtcttgagt tcgattatct atcaagggat ccttcaaaca
     6661 gcacttgtct tgggtgtata tggttttgca cttctttacc cagagcacgc tggagataac
     6721 catgccattc atgcggatgc tcttacgatg gcatatgtta ctcttggatt gattcagttg
     6781 gtgcatgcct tcaacgttaa gtctgtttac caatctatct ttaccgttgg tccattcaag
     6841 aacaaactct ttaactggtc tatcgtagct gccttcttac tcttgatggc gactgttgtg
     6901 attccaggat tcaatacctt cttcaaggta tctatcttga caccaactca atggatggta
     6961 acagtaatcg gttcaggtct catggttgtc gttgtagaaa ttgttaaatt tgttcaacgt
     7021 aaaatgggat tggatgaaaa agctatctaa tattcataaa aaataaagct cagatttagt
     7081 ggtctgagct tttttctgta ctgataatgt attttgggaa gatttcttgt cccaataaag
     7141 taatggctag tgctagtatg aggaggatgg ttttgacaag agttggtaag ttaaacagta
     7201 aggcgattgg gacgatagcc aagctgaaat agtagagaat gaagtacttg ctattctcca
     7261 ctatttggac cctatcgcct tctttaactt gaatgcttga ttttttatcc cctcgaatgc
     7321 ttagtgtaac agatggttgt gtaattggta agcttagaat tttaccttga gtaactgtcc
     7381 cagcaatttt tccatctacc aggagcggaa gtttttggga ggatagttgt ttgataggtt
     7441 ttggataatg aataataact ctcatatgaa cctccttgta atctagtttc attatagcaa
     7501 aaaagaaaaa aatgtaaacg atttctgcca ctgttttccc ctacaggctt gcttgaagaa
     7561 taagaaactg ctataatttt gaagaggtag gagtttatat gaattatctt cagaattata
     7621 ttttaagtaa aagcagttat ttgccatctg ataagttatt cattctgcaa aaagaactag
     7681 aagatttaga tgatgaggct ttaaacgttc ttatgatggt tgagatgcgt caacctcttg
     7741 tcgctcttat tttggctatt ttcttcggtg agtttggagt cgaccgtttt tatgtcggga
     7801 ataaagaatt gggatttgcc aagctgattg cttttgcagt atcttttgtt acgctattta
     7861 tcttgattgg gttcttgtta tttttgggac tttatttgtg gaagtttatt gattgcttct
     7921 taatcatgcg agcatgtaag gaagcgaact ttgagcgttt gatgctacaa atccatcaat
     7981 ataaggcttt tcagcatagc aatcagacat tttaataaga tatttttggg aataagggga
     8041 ggcttggtag ttcttccttt tctgttttta gtaggctgta aagtacttgt aatatggaac
     8101 gagctttgtc acagaagtga cattttatag aacagcattt atgatataat gataaaatat
     8161 gctagttgag aggagaattt ctatgcagac aaagaaaatg agtatgtttt tattttttgc
     8221 ctacctcctt ttacttactt ggatgattgt ctttaagatg gacttgagca tcgtatatgg
     8281 acgctatggt tatgctagta taaacttaat tccgtttgca ggaacagcag tctatgatgg
     8341 agtgctggat tttccagaaa ttctatttaa tattgtaagt tttattccct ttggtattta
     8401 tatggaaatg ctatttcgta aggcatcgtg ggtggctaat ctatatttga ttatgttagt
     8461 gagtctctgc tttgaggttc tgcaatatct tcttttactt ggggttgcag atataactga
     8521 tttactggct aacggcttag ggggagcaat cggtattaat atcatgtatg tcttgaccag
     8581 tatttggcgg gagaaggcat atgttcgtat gaatattttt tgttttgtgc taacgttttt
     8641 tattatatta ataacttatt tagctatgta ggtattgcta aatatatcca aagtttgcta
     8701 aaatgaatat aatgaatatt aaggaagaga ttattaaatt atctcaggaa atcggaatct
     8761 cgaagattgg ttttacaacg gctgatgatt ttgcttattt ggagaaatca cttcgtgcta
     8821 gtcaggaaga agggcgttcg tcaggatttg aacacaagaa tatcgaagaa cgtattcgtc
     8881 ccaaactgtc gttagaatct gccaaaacca tcatttctat cgcggtagct tatcctcgac
     8941 atttgcctgt caaaccgcag aagacccaat acaaacgtgg gaagattacc cctagttcat
     9001 gggggcttga ttaccactat atcttgcaag acaagatgga gcgtttggca cgggggattg
     9061 agaaactgac ggatggtttg gaatacaagg ctatggtaga tacaggagct ttggtggata
     9121 cagcggtcgc tcgtcgtgca ggaattggct ttatcgggaa gaatgggcta gtcatttcta
     9181 aggaatttgg ttcctatatg tttctaggag aattagtgac caacctggac atcgaaccag
     9241 atcaaccagt agattatgat tgtggtgatt gcaatcgctg tgttgcagcc tgtccgacgt
     9301 cttgtctatt aggtgatacg accatgaatg cccgtcgttg cctgtccttt cagacacagg
     9361 ataaggggat gatggacttg gagtttcgta agaaaatcaa ggcggtcatc tatggttgtg
     9421 atatttgcca gatttgttgt ccttacaaca agggaatttc cagtccacct gtggtggaga
     9481 ttgacccaga attggctgag cctgagctga ttccattctt ggacttgtca aatggacaat
     9541 tcaaggagaa atttggcctg attgctggtt cttggcgggg gaaaaatatt ctccagcgta
     9601 atgccatcat agctttggcc aatagtaatg atcgctctgc cattcccaaa ctcctagaaa
     9661 tcattgataa gaaacaaaat cctgtccaca tggcgacggc gatctgggcc ttggggcaat
     9721 tggtcaaaca gcccaatgat gagatggttt cctttatcgc aggtatttca tcagacaatg
     9781 atgatgtcag agcagagcaa acggcctttc tcaacatggt caaggacttg caaatgtgat
     9841 ataataaaga ttatgaataa aaatgaggtg tatatggata cagcagaaat tcgccaaaag
     9901 gttgaagaac ttggcaaaaa attaacttct tttagggggt ctctttgact tagaaggtct
     9961 ggaagaagag attgccattc ttgagaacaa gatgacggag ccagatttct ggaatgataa
    10021 tttggcagcc cagaagacgt ctcaggagtt gaatgaatta aaaaatactt atggaaattt
    10081 ccatcagatg ttggatttat atgatgaatc tgaaattttg ctagattttc tagcagaaga
    10141 tgagtctgtt cgggatgaac tagttgaaaa gttagcggaa ttggacaaaa ccatgactgc
    10201 ttacgaaatg accttgctct tgtcagaacc ctatgatcac aataacgcta tcttggaaat
    10261 ccatccaggt tcgggtggta ccgaggcaca ggactgggga gaaatgctcc tgcggatgta
    10321 tcagcgttat ggaaatgcca aaggttttac agtagaaaca ttggattatc aagctggtga
    10381 tgaggcaggt attaagtccg taactctaag ctttactggg ccaaacgcct atggcttgct
    10441 taagtcagaa atgggtgttc atcgtttggt gcgcatctca ccttttgact cagctaagcg
    10501 tcgtcatact tcctttacat ctgtagaggt aatgcctgag ttggacgata ccattgaaat
    10561 tgaaatcagg gatgatgaag tgaagatgga tactttccgg tcaggaggtg ctggtggaca
    10621 gaacgtcaac aaggtctcaa cgggtgttcg attgacgcac attccgacag ggattgtgac
    10681 tcagtccaca gttgaccgta ctcagtatgg aaaccgagac cgtgcaatga aactcttgca
    10741 ggctaaattg tatcaattgg agcaggagaa gaaagcggca gaagtagact cactcaaggg
    10801 tgacaagaaa gagatcactt ggggaagtca gattcgttct tatgtcttta caccttatac
    10861 aatggtaaaa gaccaccgta ctggttatga ggtggctcaa gtcgataagg ttatggacgg
    10921 agacttggac ggctttatag atgcttattt aaaatggcga attagctaag aacctgtatt
    10981 tacaagtgaa gtgcttgtaa ataagagata gtttttcgct catcaaggaa gttttttgag
    11041 agaatactga ctgtattttg aaaaaacgtt ctcaaatcta aagaaaggac tgtctaagac
    11101 aggaaatcct atgggaataa tagaaatgaa agacgtttcc aagaaatatg gaaacggaac
    11161 gactgccctt cgcggagtat cagtcaatgt tgaagcggga gaatttacct acattgtagg
    11221 tccttctggt gcaggtaagt caacctttat caaactcttg tatcgggagg aaaagctcga
    11281 taaggggagt ctaaaggttg gtaaatttga ccttgcaaaa attaaaaaga gagatgttcc
    11341 tcttctacgt cgtagtgtag gagtggtttt ccaagactac aaactcttac ctaaaaagac
    11401 tgtctttgaa aatattgctt atgcgatgga agttatcggt gagaaaccac ggaatatcaa
    11461 gaaacgtgta atggaagttt tagatttggt tggtttgaag cacaagattc gctctttccc
    11521 aaatgaactt tcaggtggtg agcaacagcg gattgctatt gcccgtgcca ttgtaaataa
    11581 tcctaaagta ttgattgctg atgaaccaac gggtaacttg gaccctgaaa actcttggga
    11641 aatcatgaat ctattggagc gaatcaattt gcaggggaca acggttctta tggcaactca
    11701 taatagccag attgttaata ccctccgcca tcgggtaatc gcgatcgaag acggtcgagt
    11761 agtacgggat gaagaacaag gagagtatgg atacgatgat tagacgattt tttagacact
    11821 ttattgaatc attgaagagt ttgaagcgaa atggttggat gacaattgca gctatttcat
    11881 ctgtagctat tactcttact ttggttggtc tatttgcttc agttatttta aacacagcaa
    11941 agcttgcttc ggatttggaa cagaatgtac gtatcaatgt atacttgaga gcgaactcaa
    12001 ctgatcaagc tgagacgatt gtgaacgaag caggagagac ggtagctaat ccagattatc
    12061 aaaaggtgta caatcagatt acagccatag aaaatgtaca atctgttact tattcaagta
    12121 aggatgaaca gttgcagaaa ttaacagcta ccttgggaga cacttggaac ttattccaag
    12181 gagatgccaa tccactttac gatgcttata tcattgatac gactgaaccg caagatgtaa
    12241 aaactgttgc agcagagatt gctaaaattg atggtgttac agaggtacgg gatggggaag
    12301 ttgaaacgga acgtattttt aaattagcta atctagttcg tacatgggga ttggcagcaa
    12361 caggcttatt gctgtttact gctgttttct tgatttccaa tactattcga attaccatta
    12421 tttctcgtag ccgtgaaatt cagattatgc gtctggttgg agctaaaaac agttatatcc
    12481 gtgggccatt cctctgggaa ggtgcctggg taggtctcct cggagctatt ttgccttctg
    12541 ctttagtcta ttctctttat aagatgatct atacttcagt aaatgctagt cttgctagtc
    12601 aagatttgtc tctgattagt atgaatgtct ttgttccagg aatgattggc gcactttttg
    12661 taattggtat tattattggt tccttgggat cagtcatttc catgaaccgc tacttaaaaa
    12721 tttaatagac aagctacatg taaaaaggtc tgcacaataa tgaggcagac ctttttgtta
    12781 aggatgtcta tggcgagaag attcgttcat ctcttgctta atagcacagt cgcaggaaca
    12841 atctccgcag gatccttttc ctttgatgag actgcgaatc gcaagagaaa ataagatgac
    12901 gatgatgaga aagaggataa gtgtgggcat taatattctc ctttttctaa tgtttttagc
    12961 gtgacgattg gaagagtatt ggttggtttt ttcactaggc tgtggatggc tgtgataatt
    13021 agaaagatag cgataaatgt ccagacggtt ggtaattgtc cgtagataag tacaagacca
    13081 atctgataga taaccaaact acttgcatag gctaaacctg tttggaaacc gatagcaatc
    13141 catgtccact tggcttctcc catttcacgg tgaatggcac caatagccgc aaaacaaggg
    13201 gcacagagga gattgaagac taggaaggag taagctgaca atgctgtata gtcttgttgt
    13261 aaaagccccc agagttcggg attttcttcg gttgtttctc ctaatttata gaggatacca
    13321 aaggtggcaa tgacggtttc cttggctaag agacctgtaa tggctgcaac agttgctcgc
    13381 caatttccga aaccaagtgg ggcaaagagg agagaaaaac tgcgtccaat ggaggctaag
    13441 atactctcat ctgtttctac catttgtaaa gaccaattat aggaactgct gaaccaaata
    13501 atgatgttgg tcacaaatat aatggtacca gctcgcttga taaaactaag tgccttatcg
    13561 aatgcatatc ggagaaccgt actgagtttc ggtaagtgat aagatggtaa ttccatgata
    13621 aaggggcttg cgactccacc aagttgcttg gtctttttta gtgccattcc ggataggacg
    13681 atggcagcca taccgacaaa ataggcgctt ggagcaatcc aaggattgtc aggaaagaaa
    13741 gcaccgacta ccagcgaaat gataggaagt ttggcagaac agggcatgaa ggttgcggtc
    13801 ataatggtga tttttcggtc ctgttcattt tcaatagtcc ggcttgccat gactccagga
    13861 acgccacagc ctgtggaaat caacatggga ataaaggatt tgcctgatag accaaaacgt
    13921 ctaaaaatcc tatccatgac aaaggcgact cggctcatat agccgatgtc ttccaagatt
    13981 cccaagcaga cgaagaggac gaaaatttgt ggtacaaaac ctagaatggc tccgcatcct
    14041 gcaataatgc catcaaggac cagagattgt agccagccag cgatttcaaa tctatctaga
    14101 ttttcctgaa taaggcctgg aacaagctct ccgaagagga catcattcac ccagtcagtc
    14161 cccattgtac caaccgtttg aatagacagg aagtaggtca gccacatgac tgccgcaaag
    14221 atagggaggg caagaaaacg attggtaaaa agtcggtcga tgttatcaga caggttaaaa
    14281 gtcttatcgc tatcttttgt gaccagttct gtgattttct cgataaattg gtagcgttga
    14341 ttgacgataa tggcttccat atcgtctgcg taaatttttt ccaaaatggc aatgatttcg
    14401 gtcaaatcaa gcatgtcttg cgcattgagt tgcaattggt ctagaatgac ttgatcttgt
    14461 tcgagtagct taatggcgta gaagcgtttt tgatgatttg ggacggaatc cgataatagg
    14521 tcaataactt gggcaagacc ggcttcaaat tgcttatcat actgaggata gattggctcg
    14581 gcttgttgat tggtaatttt ttctacttca tggaggagct gggatatacc tagcttcttt
    14641 agtgcactga taggctgtac aggaactccc agttgatagg agagctggtc gctgtttatt
    14701 gagcgacctt gacttttcag ggcgtctgtc atattgaggg cgataacaat aggaaggccc
    14761 atttctaata actgcaaggt cagatagaga ttccgctcca agtttgttgc atcaaggacg
    14821 ttgaggatag cagctggttg gtccgctatc aaataatctc tggcaacccg ttcttctggt
    14881 gtgtaggggg acagcgagta gatacctggt aaatcttgaa ttagtaaatg ttttcgcttt
    14941 ttgatcgttc cagattttct ttcaacggtg actccgggcc agttaccaac acgttggttg
    15001 gtccctgtta ggaggttgaa aagagttgtt tttccgctat ttggattacc tgcaagggcg
    15061 atgtgttttg tcatttcctc acctcgcttg tgatttgaac accaatcatt tgcgcttcgg
    15121 atttgcgtag tgtcagctcg tatcctctca aggtaatttc gattggatcg cccaggggag
    15181 cgactttttt gagaaatagc tgcgtgttct ttgtaagtcc catatccatg aggcgacgac
    15241 ggagtgcacc tgttgcatag atgtccatga ccttagctga gctaccaata ggtgcctcag
    15301 atagattgat tatttccgta gctgactttt ctattaaaac cagagattca ataatttctc
    15361 tgtctagggc tagacggctt gccttgactt tgaaaattgc attggtcttg gtttttgaaa
    15421 tgatgcgaat ctcttctccg acttttaaac ccaagtgcgc taagtgttta cgaatatcat
    15481 cttgcgttag acaatctttg actaaataag attgtccaac ttgaaggttt tgcagtttca
    15541 tatcgtttcc tttgtgattt ttataacaat agtatagcac aattccttaa aagtgtcaga
    15601 taatttttag gaatggctta ttttctcttt atttttctca atatttccaa aaaatccatt
    15661 gacttttttt agagatatga tatactagtc aagtactgct ggtttagctc agtcggtaga
    15721 gcgcatccat ggtaaggatg aggtcgccgg ttcaatcccg gcaactagca tcgaatacta
    15781 tcgaaagacc ttgagtgaca aggttttttc ttatttttgt cctaaattcg cccttattgc
    15841 caaaaaatcc cttgacatga atgtttatta tggtagaata gggcttagaa atatgaaagg
    15901 agtaaaacaa atgaacaagc aacttgtcat tcagcaagct attttttatc atacactcct
    15961 ttctaagcag gaaaacaaag ctcattgatg aacttttgtc gtttttcgtg ggctatattt
    16021 tgaaacattg agacaatcaa gcactgtggt catggtgtga taaaaattgt cgactatgtt
    16081 caatagccgg tctatttttg tggacttacg caagcctagg tggagtgaga tgcttccctg
    16141 ggcttttttg tgcgcttaga aaggtaatac tcttcgaaaa tcaaaattat ccgttgtcaa
    16201 cttgccttga tgaactccag ttctatcttc gacatcgttt tctaggctac ttttgatttt
    16261 cattgagtat aacgtctatc aattgttgct gattgaataa agtggcaaat gataggttat
    16321 agatttgtaa aggagatata tgtttcgatt aatttttgac tatatcaagc gtcataagtg
    16381 gctctatcta ttggttgctg tgaccttgat tatttatgac gccacacttt tgctaccaac
    16441 gcaaatcatt cagcggatgg tcgatatact aaccaagaat gaattaacac aggccatatt
    16501 ggttcaggag atgaccttat tgttattggt aacagtgctg aactatgcaa cagccttcat
    16561 ttggcatctg aaattatttc aggcctctgt caatttcaag tttgatatgc agcaacgtgc
    16621 ctttaaaaaa ttagtgacca tgcggactcc attttatgaa aaattccgct caggagatgt
    16681 catgactcgt ttttcaacgg atgtggatgg cctgatggag atggtgggtt acggtcttat
    16741 gattgtcgtc tatgcaggtg gtatgttggc ttttatcatt cccacgatgt tctttattga
    16801 ttggaaaatt tcgctagtag ctttactacc gatgctgttc atgacactat gtatattttt
    16861 tatcgggaga aaacaagatg aggcgattga tgccaataga gaagctgtag cccagctcaa
    16921 taatgaagtt ttggaggtca tcgaaggtat tcgagtgact cgggcttata gtaaaaaagc
    16981 aaatcaaaag gctcaatttc aagctcgtac caagcaattg gctcaaggtg gggatcgcat
    17041 cacttccttg caatcattgt ataatccatt agccacagtc tgtttgggat tgtctactat
    17101 tttcgtactt ctcatggggg cacaagccgt gaaagcaggg caactgacct tgggacaagt
    17161 aattgccctt caattgtatg tcggttcgct tttagagccg ttttggacac tagcagattt
    17221 catccttgtt tatcagactg gtaagacatc tttcgaaaaa ctgcaagagc tgattgagac
    17281 gggtgatgat ttggaggctg atggttctaa ggaaattgca gagctgtcta gtatttcctt
    17341 caaaaattat agctttagtt atccacaggc ggaaagagcc agcctacagg acatcaattg
    17401 gacactgaaa gcagggcaaa ctgtaggtat cgttgggaaa actggttcgg gaaaaacgac
    17461 cttggttcgc caattcttac gccagtatcc gattggtcgg ggaaattttt tcatcaatca
    17521 tcaatctgtt ttagacttta aacgttctag tatagaggaa aaaattggct atgttccaca
    17581 agaacacatt ttgttttcaa ggtctgttgg agaaaatatt gcccttggga aagtagctag
    17641 tagctctgag gagattgaac aagctattgc cacagcagct tttagtcaag atttaaaacg
    17701 gatgactgat ggtcttgata ccatgattgg tgagcgtggt gtgtctattt ctggcggtca
    17761 gaaacaacgg atttccattg cccgtgcttt cctgcgagag ccagatttgc taattctaga
    17821 tgattctttg tcggcagtgg atgctcggac agaacgccag attatccaaa atattcaaaa
    17881 agaacgtgca ggcaaaacca atgtcatcgt gacccatcgt ctatctgctg tcaaccatgc
    17941 agactgggta ctagtcttgg atgaggggcg tatcgttgag aaagggcgtc cagctgattt
    18001 gcttgcccaa agaggttggt actatgaaca ataccaacga caacaaagcc aggaaggagg
    18061 agagtaatga ctattttagg ttttttatta aaacaaatcg gtcgtgttaa gtggctattt
    18121 atagtagctg tcggcttcta tctattggct tcgaccatgg ttcgattggc tcctctgctg
    18181 attcagcagg cgattgatgg gccgattacg gatttgagca agggtttgcc ctttgatgaa
    18241 gctgttttct taaaccagtc agctcaatac atgggaatga ttattctggg agctattggc
    18301 ttctacctat ctatgcgttt actcatgcat tgcgccaatc gtattgctga aaatctacgt
    18361 aatcaagctt atgatgttat gcaacggttg cctatttctt attttgatga taagcccgct
    18421 ggaaaaattg caactagaat tgtcaatgat acagaaactc tgcggacaca attttatggg
    18481 accttagtta atgcctttaa caatattgtt cgtttgctct ttacatatgg cgtcctattt
    18541 tacatgaata ggagtcttgg ttggctgatg ttactgctaa ttcctctata tattgggatt
    18601 caatttgctt ataaaaaaat gactgacaag cccatgaagg acttctatga tgccagaagt
    18661 gatgtgaata ctcaggtaaa tgaaaccatg aacggtgcta gtttaattca actctttgga
    18721 caagaagagc gtatcatgaa agaatttgaa gtgactgctg acaagatgcg tcgggcagat
    18781 aataagatta tctgggccca gtctcttgcc acgtggaatc tgagtggttt tctgcaaaat
    18841 ttagtcattg ctgcgatttt aacggtagtc ggctatcaat ttcttgcggg acaggatgga
    18901 gttacagctg ggaaactctt tgtctatgtc aactatatcg aaggggtctt cattgctttg
    18961 ggagcgctgg ttcaacagtt cccaaatatg cttcgatcgt ttgaaacagg taagcgtcta
    19021 atggcccttc tagaagaaga ggtggaggac gactgtgatc gtattttgga agttgaacaa
    19081 gggcaagtcg tgtttgaaca cgtgaacttt agctatgaag aacacagacc agttttgaaa
    19141 gacattacca ttcgggcaga aaaaggagaa acagttgctc tggttgggca tactgggtct
    19201 ggtaagtctt ctattatgaa cctcctctat cgtttctatg atccacagga gggacgtgta
    19261 ctgattgatg agaaaaacat ccgtgattat tctcgtgaaa gcctgcgcag tcatatgggg
    19321 attgttttgc aagatcccta tctctttaca gggacgattg ccagcaatgt ttccatgaat
    19381 gaagaggaag cagatagaac aagaatcatg caggcgctag aaaaagttgg tgcagggcct
    19441 atgctatctc gcttagagaa agggattgat gaaccggttg ttgagaaggg ggcggccttt
    19501 tcaagtgggg aacgtcagtt gattgccttt gcgcggacac tctactctga tcctaaaatt
    19561 ctgatattgg acgaggcaac ttctcatatt gatacggaga cggaagaaat tatccaacat
    19621 gctatggaag tggtgaaaga aggtcggaca acctttatca ttgcccaccg actttctacc
    19681 attcaaaatg cagaccagat tcttgtctta gatcaaggtc gaattattga acatggaaag
    19741 catgaagaat tggttgcacg tggtggagta tatgctcaaa tgcatgagat tcaagcaaag
    19801 gtgtaataag cagttaaaaa ttccaagctt tggctggaat tttttgctat aattatgtga
    19861 cttttagtcc gtcacgcc
//
LOCUS       DATUCG010000030        19831 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.031, whole genome shotgun sequence.
ACCESSION   DATUCG010000030 DATUCG010000000
VERSION     DATUCG010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19831
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.031"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            <1..243
                     /locus_tag="VCA20_000851"
     CDS             <1..243
                     /locus_tag="VCA20_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HEP1834699.1"
                     /translation="FNVCKWLTSHVAIKTSKLKQTYKICFSDAVYACRKFLREELTSF
                     QLETYIAKHLSIIRPNRTFQRKIKSQAPVSFTYRIS"
     gene            complement(539..2287)
                     /locus_tag="VCA20_000852"
     CDS             complement(539..2287)
                     /locus_tag="VCA20_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1834700.1"
                     /translation="MLSKNKQASVLLRLLSYLKAYRLLTVLALIFLLLTTVVRSLIPL
                     LASYFIDHYISSISETAVVILAAYFALYLLQMLLQYFGNLWFAKVSYSIVRDIRRDAF
                     GRMEKLGMAYFDQTPSGSIVSRITNDTESISEMFSGILSSFISALFIIITTLSTMFAL
                     DWKLTCLIILFLPLIFILVDQYRKRSAPIVAKTRSLLSSINSKLAESIEGIRIIQAFS
                     QEDRLKEEFEAINQEHLDYASRSMALDSLFLRPALSLVKILAYALLMTYFGLDWAQAG
                     ISAGLIYAFIQYVNRLFDPLLDVTQHFSTLQTSMVSAGRVFTLMDQTIEEPLQLDRGA
                     KIDKGHIVFEDVCFSYDGKRQILDKVSFEVRQGQTIAFVGATGSGKSSIINVFMRFYE
                     FQSGRILIDGQDIRSFSQEELRSAIGLVLQDPFLFHGTIASNIQMYQDISQEEIEEAA
                     RFVDAAPFIEKLPEKYDHLVTERGTAFSTGQRQLLAFARTMASQPKILILDEATANID
                     SETEQIVQTSLEKMRKGRTTIAIAHRLSTIQDADCIYVLDKGKIIESGNHEELLALKG
                     HYHRMYQLQAGQLAAT"
     gene            complement(2277..4019)
                     /locus_tag="VCA20_000853"
     CDS             complement(2277..4019)
                     /locus_tag="VCA20_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEP1834701.1"
                     /translation="MKIIKQLWWFFSLEKKAYLIGILSLCVVSLLNLLPASIMGQLID
                     QIASGQLTEHRLLLGVAGLILSALAMYGLRYLWRMNILATSYRLGKIMRARLFDHFMH
                     LSPSFFQQHRTGNLMAHATNDINALTRLAGGGVMSFVDATVTALVTLATMSVSISWQM
                     TLVAILPLPFMTLTTNFLGRRTHENFKASQAAFSELNNKVQEAVSGIKVTKSFGYQEQ
                     ETAAFQEVNQAAFLQNIKTMRYDALFNPTVLFFIGLSYLLTLLVGSHFISQGQVSLGQ
                     LVTFMTYLDLLVWPLMAIGFLVNISQRGDVSYNRIQTLLGISSEVIETDQPLPAPSNG
                     EIVYEISEFAYDNIPVLQDIQFHIKKGQTIGLVGPTGSGKTSLLKLLMREYDVTKGQL
                     LLNQENIKNYKLADLRRLIGYVPQDQFLFATSVTENIRFGNPDLSLDQVEEAARAVHV
                     YEDIQDMPDCFETMVGEKGISLSGGQKQRLAMARAMILNPDILLLDDSLSAVDAKTEH
                     AILETIKQERLGKTTIITAHRLSAIVHADLILVLEDGKIVERGRHQELLDEKGWYYDT
                     YMMQQLEKEESDAL"
     gene            4276..4542
                     /locus_tag="VCA20_000854"
     CDS             4276..4542
                     /locus_tag="VCA20_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEP1834702.1"
                     /translation="MKAIVTVVGKDKSGIVAGVATKIAELGLNIDDISQTVLDEYFTM
                     MAVVSSDEKKDFTKLRAELEAYGQALNVKINIQSAAIFDAMHNL"
     gene            4554..5891
                     /locus_tag="VCA20_000855"
     CDS             4554..5891
                     /locus_tag="VCA20_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HEP1834703.1"
                     /translation="MDIRQVRETIEMIEEQHFDIRTITMGISLLDCIDSDIDKAAEKV
                     YTKIVTKAKNLVAVGDEIAAELGIPIVNKRVSVTPIALIGAATDATDYLPLAHALDKA
                     AHEIGIDFIGGFSALAQKGYQKGDEILINSIPQALAQTSKVCSSVNIGSTKTGINMTA
                     VRDMGRIIKETAEASDMGAAKLVVFANAVEDNPFMAGAFHGVGEADVVINVGVSGPGV
                     VKRALEKVRGESFDVVAETVKKTAFKITRIGQLVGNMASERLGVKFGIVDLSLAPTPA
                     VGDSVARVLEEMGLETVGTHGTTAALALLNDAVKKGGVMACNQVGGLSGAFIPVSEDE
                     GMIAAVQNGSLNLEKLEAMTAICSVGLDMIAIPETTPAETIAAMIADEAAIGVINQKT
                     TAVRIIPLGKEGDMIEFGGLLGTAPVMKVNQASSVDFINRGGQIPAPIHSFKN"
     gene            5966..6661
                     /locus_tag="VCA20_000856"
     CDS             5966..6661
                     /locus_tag="VCA20_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEP1834704.1"
                     /translation="MADVRLYISRHGKTMFNTIGRVQGWCDTPLTKAGEEGIRELGLG
                     LKDAGLDFKLAVSSDLGRTVQTMTIAQRELGILGKIPYYQDKRIREWCFGSFEGMYDA
                     ELFQGVLPRLKGTVDATGMSFAEIAAGIQEADTAGWAESWEVLSNRILTGFESIAQDL
                     EKQGGGNALVVSHGMTIATLAHLLEPERGANIFLDNGSITVLKYENGKLLIEAVGDLS
                     YRKRGAELIAQGK"
     gene            complement(6742..7434)
                     /locus_tag="VCA20_000857"
     CDS             complement(6742..7434)
                     /locus_tag="VCA20_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP1834705.1"
                     /translation="MIHYFKADLFKIQKEQRLTISLGILALLSFLSAFLLHGNEDFTS
                     SLIQLLSQFITLFFIVPANLFFGEDFTYRTINNIIIKQQTRKRVFFYKVLATLVLDLA
                     YILLAYLLSSSVGLLLSDPVDVAVIIHSFIAQTPLFICISLLSILIFVKSNKVNQAYL
                     AFSLISLLFDNISNLITSNLLHMKLPTDYFLFLSLQQGENISRLSMSVSFMATILYLY
                     LSYFIFSRKELK"
     gene            complement(7418..8314)
                     /locus_tag="VCA20_000858"
     CDS             complement(7418..8314)
                     /locus_tag="VCA20_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEP1834706.1"
                     /translation="MSSLLSTQELSKQYGKQKAVHQVSLTINKGEICGLVGENGAGKT
                     TLLRMLSGLISQTSGTITSSKDCRIGALIESPALQPNLSAIDNLRYMALQLNLKQADE
                     KILETLAIVGLEDVDPKKKSKDFSLGMRQRLAIALAILDDPDFLILDEPINGLDPVGI
                     KEMRSIILNLRNQYGMTILISSHILSELEMVVDRYIIMHKGLIVKEFSKRELEQTLEE
                     QLYLQTNNHPKTFTILEEQGIAYKIDKDYISLSSDTNVMSLIHLLINHEIEVKEIFKQ
                     QMSFEDYYLTLLQEGEEHDTLL"
     gene            8412..9272
                     /locus_tag="VCA20_000859"
     CDS             8412..9272
                     /locus_tag="VCA20_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017769268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEP1834707.1"
                     /translation="MGTYGKIFKLIRESKNMSLKEVAGDFVTPAQLSRFENGKSNLSV
                     DTFFYCLRRMNVVEGEFSTLYQSYFQIDNLFSSEEFVRAVYSHNAQYMDDRILYHQAE
                     YDRDGRPFDRVMVAAFHIFKQQWCDPDYDVSEDEKAILSDYLMSIDDWGKFELWIFGN
                     CSRGLPSKMLEVLGMEVLNRTKFYMDITENRDNVYKVLINISSSLLDRGEEIAAIRFL
                     KILDSVNIMEKNMRDRLLLKLLKARLSYMNGNDGGLEIMKECLHIAKFLDCYDLIHQI
                     NKEIEKVTDM"
     gene            9536..9799
                     /locus_tag="VCA20_000860"
     CDS             9536..9799
                     /locus_tag="VCA20_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834708.1"
                     /translation="MMKKLLVLGALAMLGFVTFGLLTTNSSSASAETEGKVVLFGKVD
                     FSDDKEQDTDEEREEVATESDDASWLENFITTHKTPFSKTIKK"
     gene            9899..10222
                     /locus_tag="VCA20_000861"
     CDS             9899..10222
                     /locus_tag="VCA20_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEP1834709.1"
                     /translation="MNDKLKRVYVPMTETAFYILFHLQEERHGYDITQKTKEVTAGQV
                     VISPGTMYGTLSKMEKDGLIAFVREEDKRKFYQITATGREILDIELARIERLYRNSKG
                     ESYGD"
     gene            10212..10727
                     /locus_tag="VCA20_000862"
     CDS             10212..10727
                     /locus_tag="VCA20_000862"
                     /inference="COORDINATES: protein motif:HMM:NF022641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HEP1834710.1"
                     /translation="METKTEIHFFFLPDFEKEEHYLAEQHRQGWKFQKNKFGFFYIFE
                     KCQPEEVIYQLDFKPNGQDKEEYLQMFADYGWEYVGDCNHFSYFRKNESLGEVELYSD
                     RQSKFEMIDRIITRQFLLVSSLFVFFILLFYVLKLPAVMIGMAVADLPVLLYCSIGLM
                     RLRQKYKEIGK"
     gene            10724..10948
                     /locus_tag="VCA20_000863"
     CDS             10724..10948
                     /locus_tag="VCA20_000863"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834711.1"
                     /translation="MTKQKRIATLINALAIFAVIFLNRNSFISLIAITLIMILNFWNL
                     IEYEGRKVIQKYLWKRTFIIWTIHHSRSSD"
     gene            11100..12134
                     /gene="hrcA"
                     /locus_tag="VCA20_000864"
     CDS             11100..12134
                     /gene="hrcA"
                     /locus_tag="VCA20_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEP1834712.1"
                     /translation="MITQRQNDILNLIVELFTRHHEPVGSKTLQDMIASSSATIRNDM
                     AKLEQLGLLEKAHTSSGRMPSRAGFQYFVNHSLNLEHIDEEDVYQVVKAFDFEAFKLE
                     DILERASQVLADLTGYTSVILDVEPTSQQLTSFDIVQLSSHDALAVLTLDQSKPVTVQ
                     FAIPKNFLARDLEVLKRLVDERFVGQTVLSIHYKLRTEIPQVVQRYFTTTDNILDLMD
                     YIFSNLFQESVFINGKVASLTYGNLATYQLLDSPQVLASELRQGLAPNQQTSISVAEH
                     REPALADVTIIHHRFPIPYRGMAQMSLLGPIDMDYRRQMSLINIISRVLFMKLTDYYR
                     YLSSNHYEVN"
     gene            12284..12796
                     /gene="grpE"
                     /locus_tag="VCA20_000865"
     CDS             12284..12796
                     /gene="grpE"
                     /locus_tag="VCA20_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEP1834713.1"
                     /translation="MSEEIKNEEIVEEVETTEEVVETPEKSELDLANERAEEFENKYL
                     RAHAEMQNIQRRANEERQTIQRYRSQDLAKKILPSLDNLERALQVEGLTEDVKKGLEM
                     VQESLIQALKEEGVEEVATDVFDPNLHMAIQTVPATDDCPAEHIAQVFQKGYKLHERL
                     LRPAMVVVSE"
     gene            13014..14837
                     /gene="dnaK"
                     /locus_tag="VCA20_000866"
     CDS             13014..14837
                     /gene="dnaK"
                     /locus_tag="VCA20_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEP1834714.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTIISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     EYLGEKVTKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTDK
                     DEKILVFDLGGGTFDVSILELGDGVFDVLATAGDNKLGGDDFDQKIIDHMVAEFKKEN
                     GIDLSADKMALQRLKDAAEKAKKDLSGVTSTQISLPFITAGAAGPLHLEMTLTRAKFD
                     ELTYDLVERTKVPVRQALSDAGISLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQTIVIQSNSGLTDEEIDRMMKDAEANAEADKKRKEEVDLRND
                     VDQAIFATEKTLKETESKGFDAERDQAQAALDELKAAQEANNLDDMKAKLENLNEKAQ
                     ALAVKLYEQAAAAQQAAAGQEGPQTANNAGDDVVDGEFTEK"
     gene            15537..16673
                     /gene="dnaJ"
                     /locus_tag="VCA20_000867"
     CDS             15537..16673
                     /gene="dnaJ"
                     /locus_tag="VCA20_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEP1834715.1"
                     /translation="MNNTEFYDRLGVSKNASPDEIKKAYRKLSKKYHPDINKDPGAED
                     KYKEVQEAYETLSDPQKRSAYDQFGPAGVNGGFGGGAGGFGGFDGAGFGGFEDIFSSF
                     FGGGGATRNPNAPRQGDDLQYAVNLKFEEAIFGVEREVSYNREATCRTCTGSGAKPGT
                     SPVTCGRCHGSGVINVDTQTPLGTMRRQMTCDVCHGRGKQIKDPCTTCHGTGHEKQAH
                     TVTVKVPAGVETGQRIRLAGQGEAGFNGGPYGDLYVVIQVQPSDKFEREGTTIHYKLN
                     LNFVQAALGDTVHVPTVHGDVDMVIPEGTQTGKTFRLKGKGAPSVRGGVIGDQYVTVN
                     IVTPTGLNDRQKAALKEFAAAGNIDVKPHKKGFFDKVKDAFDEL"
     gene            16766..17961
                     /locus_tag="VCA20_000868"
                     /pseudo
     CDS             16766..17961
                     /locus_tag="VCA20_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943088.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
     gene            18084..18959
                     /locus_tag="VCA20_000869"
     CDS             18084..18959
                     /locus_tag="VCA20_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEP1834716.1"
                     /translation="MLKKVLASALLVSTLTLAACSSSSSNTSSSSAEQTQWEKVQEAG
                     VLKVATPGTLFPTSYYNDSKELVGYEIDMINEIGKRLKLEIEYQEIGVAEAFTAVDSG
                     KVDISVNNFDMTEARKEKYNFSIPYKYSVGGYIVLEDGSSGIEAADLSDWTGKKAGGG
                     AGTQYMKIAEKQGAEPVIYDNVTNDVYLRDVSTGRTDFIPNDYYTQVMAIKWANANFP
                     DIKVKMGDAKYNPTEQGIVMSKADTSLKEKIDEALEAMKADGTLKAISEKYYAGQDLT
                     VPVENADKLPVIEVE"
     gene            19050..19721
                     /locus_tag="VCA20_000870"
     CDS             19050..19721
                     /locus_tag="VCA20_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEP1834717.1"
                     /translation="MDINWQAVFNADIAREAIPQILEGLPYTLSLSLIGFALGTFGGF
                     FVALMRMSKFGPLRWLAMAHISLMRGIPLMVLLFFIYFGLPFMGLQLDAISASIIAFT
                     SMSSAYISEIIRASLSAIDKGQWEAARSLGLRTNVIYRKIIIPQAFRIALPPLSNVLL
                     DMVKSTSLTAMITVPEIFNKAKIVGGAKSDYMTVYICVALIYWVICTLYAFGQLQLEK
                     RLATY"
BASE COUNT         5868 a         3944 c         4618 g         5401 t
ORIGIN      
        1 tttaatgttt gtaaatggct aacctcacac gttgcaatta aaacatcaaa gttaaaacag
       61 acttataaaa tttgtttttc agacgctgtt tatgcctgtc gaaaatttct tagagaagaa
      121 cttacttcct tccaattaga aacctacatt gctaagcatt tatccatcat ccgacccaat
      181 cgaaccttcc aaagaaagat aaaaagccag gctcccgtaa gcttcactta tagaatatca
      241 taatatagtg gcttgaatta ggaaaaggac agccttaagt tatagtgtta ataacccact
      301 cctaaaataa ctttagagtt ctcaaattga caaacttaga tacgtcccgt ttctaattca
      361 cttgactata gttaaaaaat tgggttaaaa accttccttt ttgtgcgccc taaatacaaa
      421 aactattccc gttttgtcat caattgctca gacgggaata gtttctttag ctgcttagtt
      481 gcttaactta atgacattgg gtccattttg gaacttggct tttgtcatgg agtggtttct
      541 aggttgctgc taactggcct gcttgtaact ggtacatacg gtggtagtga cccttgaggg
      601 ctagaagttc ttcgtgattg cctgactcga tgatctttcc cttatccaag acataaatac
      661 agtcggcatc ctgaatggtc gataggcggt gagcgatggc aatggttgtc cgcccttttc
      721 gcattttctc aagagaagtc tggactatct gctccgtctc agaatcgata ttggcggtag
      781 cctcgtctaa aataagaatc ttaggctgac tggccatggt gcgtgcaaag gccagaagtt
      841 gccgttggcc tgtcgaaaag gcggtacccc gctcagtgac caggtggtcg tatttttcag
      901 gaagtttctc aataaaggga gcagcatcca caaatcgagc cgcttcctca atctcctcct
      961 gggaaatatc ctgatacatc tggatattgg atgcaatggt cccgtgaaag aggaagggat
     1021 cttgcaggac aaggccaatt gcggaccgca attcttcctg cgaaaagcta cgaatatctt
     1081 gaccgtcaat cagaatccgc ccagactgga attcatagaa acgcataaag acattgatga
     1141 ttgaggattt tccagaccca gtggcaccca caaaggcgat ggtctgcccc tgtctcactt
     1201 caaaggaaac cttatccaga atttgccgct tgccgtcata ggaaaagcaa acatcctcaa
     1261 agacaatatg ccccttgtca atcttggccc ctcggtccag ctgtagtggc tcctcgatgg
     1321 tctggtccat gagcgtaaag actcgtccgg cagataccat agaggtttgg agggtggaaa
     1381 aatgttgcgt gacatccagc aagggatcga aaagacgatt gacgtactgg atgaaagcat
     1441 agatcagacc agcagaaatc ccagcctgtg cccagtccaa accgaaatag gtcatgagca
     1501 gagcgtaggc caaaatctta acaagtgaaa gagcagggcg gagaaagagg ctgtccaagg
     1561 ccatggagcg actagcataa tccagatgtt cctgattgat agcctcgaac tcttctttca
     1621 atcgatcttc ctgagaaaaa gcctggataa tccgaatccc ctcgatactc tcagccagtt
     1681 tgctgttgat agaactcagc aggctcctgg tcttggccac aatgggagca gaccgcttcc
     1741 ggtattgatc aaccaagatg aaaatcaagg gaagaaagag gatgatcaga caggtcaatt
     1801 tccaatccag agcaaacatg gtcgaaagtg tggtgatgat aataaagagt gctgagataa
     1861 aactggataa aatccctgaa aacatctctg aaatgctttc ggtatcgttg gtaatccgag
     1921 agacgataga gccactcgga gtctgatcaa aataagccat tcccaacttt tccattctcc
     1981 caaaagcatc cctgcgaata tccctgacaa tgctgtagga aactttagca aaccagagat
     2041 ttccaaaata ctggagcagc atttgaagca gatagagggc gaagtaggcc gctagaataa
     2101 ccacagcagt ctcagaaata gaagaaatgt agtggtcgat aaagtaagaa gctagaagag
     2161 gaatcagact gcggaccacg gtagtcagta gcaaaaagat caaggcaagg actgtcagca
     2221 gtcgataggc tttgagatag gacaagagtc gcaacaaaac actagcttgc ttgtttttag
     2281 agagcatcac tttcctcctt ttcgagttgc tgcatcatat aggtatcgta gtaccagcct
     2341 ttttcatcta gtaattcttg gtgacgacct cgttctacga tttttccatc ttcaaggacc
     2401 agaatcaaat cggcatggac aatagctgac aggcgatggg cggtgataat agtcgtcttg
     2461 cccagtcgtt cttgcttaat ggtttcaaga atagcgtgtt cagttttagc atccaccgct
     2521 gagagcgagt catccaagag caggatatct ggattgagaa tcatagcacg ggccatagcc
     2581 aaccgttgct tttgtccacc agaaagggaa atccccttct cgccgaccat ggtttcaaag
     2641 caatcaggca tatcctgaat atcctcatag acatggacag cccgagcggc ctcttctacc
     2701 tggtctagtg agaggtctgg attgccaaag cggatatttt cagtaacgct tgtcgcaaag
     2761 aggaattggt cttgaggtac atagccgatt aagcgtcgca ggtcagctag cttgtagttc
     2821 ttgatatttt cctgattgag caagagttgc ccttttgtca catcgtattc tcgcatcagg
     2881 agcttgagca ggcttgtctt cccagaacct gttggcccca ccagaccgat tgtctgccct
     2941 tttttgatgt gaaactggat gtcttggagg actggtatgt tatcataggc aaattcgcta
     3001 atctcataga caatctctcc attgcttggt gcaggcagcg gttggtctgt ttcaatgacc
     3061 tctgaggaaa tgccgagaag ggtctgaatc cgattgtaag atacatcacc acgctggcta
     3121 atattgacca aaaaaccaat cgccataaga ggccagacta ggagatctaa ataggtcatg
     3181 aaagttacta gttggcccag actgacctga ccttgcgaga tgaagtggga gccgaccaga
     3241 agggtcagca ggtaggacag gccaataaaa aagaggacgg tagggttgaa cagggcatcg
     3301 taacgcatgg ttttgatatt ctgcaagaag gctgcttggt tgacttcctg aaaagctgcc
     3361 gtttcttgtt cttggtagcc aaaggattta gtgaccttta taccagaaac agcttcctgt
     3421 accttgttgt taagctctga aaaggctgcc tgtgaagcct tgaaattttc atgagtgcga
     3481 cggcctagaa aattagtagt tagagtcatg aagggtaagg ggaggattgc aaccaaggtc
     3541 atctgccagg aaatgctcac actcatggtg gccagtgtaa ccaaggcagt aacggtagca
     3601 tcaacaaagg acataacgcc accacctgct agtcgggtca gggcattgat atcattggtc
     3661 gcatgggcca ttaaattccc agtccgatgt tgctgaaaaa aggatggaga caggtgcata
     3721 aagtggtcaa acagccgagc cctcatgatt tttcctagac gataagatgt tgccaggatg
     3781 ttcattcgcc aaagatagcg gaggccgtac atggccaagg ctgacaagat gagcccagct
     3841 accccgagta gcaagcgatg ctcagtcaac tgtccgctgg caatctggtc aatcaactgc
     3901 cccatgatag aagcgggaag gagattgagg aggctgacca cacagagtga taaaatccca
     3961 atcagatagg cctttttctc aagggaaaag aaccaccaca attgcttgat aatcttcata
     4021 agtgtccttt ctgaacaaaa gaaaggttgg tagcttgtcg ccaccaacaa ataatagaat
     4081 tcggtgacac tagtgtatca cagactggta gaaatagaaa gtttgcaact taaaaattag
     4141 gcaactctag ctgtagccga atagaagaaa cagttggagt tgtaaacacg tacgatacag
     4201 gtagaaatca aaagattttt accttttttt gcatatttat gataaaataa atgaaaattt
     4261 agaagaggtg ttcagatgaa agctattgtt acagttgtcg gtaaggataa gtcaggaatc
     4321 gttgcgggtg ttgcgactaa gattgcggag ttagggctca atattgatga tatttcacaa
     4381 actgttttgg atgagtattt taccatgatg gcggtggtgt cgtcagatga gaagaaagat
     4441 ttcaccaagc ttcgtgcaga actagaagcc tatggtcagg ctttgaatgt gaaaatcaac
     4501 attcaaagtg cggcgatttt tgatgccatg cacaacttgt aaggagagat ttcatggata
     4561 ttagacaggt tagagaaacc attgagatga tcgaggaaca gcacttcgat attcggacta
     4621 tcaccatggg gatttccctc ttggactgta ttgactcgga tatcgacaag gctgcggaga
     4681 aagtgtatac caagattgtc accaaggcca agaatttggt agcggtcggc gatgagattg
     4741 cggcagaatt gggcattccc attgtcaaca aacgggtatc ggtgacacct attgctttga
     4801 ttggggcggc gacagatgcc acggattatc ttcccctggc tcatgccttg gacaaggcgg
     4861 ctcatgagat cggcatcgat tttatcggtg gtttttcagc tctcgctcaa aagggttacc
     4921 aaaaaggtga tgaaatcctc atcaactcta taccgcaggc attggcccaa acctctaagg
     4981 tctgctcgtc agtcaatatc ggctcgacca agacgggtat caatatgacg gctgtgcggg
     5041 acatggggcg gatcattaag gagacggcgg aagcttctga tatgggtgcg gccaagctgg
     5101 tggtctttgc caatgcggtt gaggacaatc cctttatggc gggtgccttc catggtgtcg
     5161 gtgaggcgga tgtggtcatt aatgtcggcg tgtcgggacc tggtgtggtc aagcgtgccc
     5221 ttgaaaaagt gcgtggtgag agctttgatg tggtggcgga gaccgtcaag aagaccgcct
     5281 tcaagattac ccgtatcggt cagttggtcg gtaatatggc cagtgaacgc ctgggtgtca
     5341 agtttggtat cgtagacctg tcgcttgctc caacgccagc ggtcggcgac tcagtagcac
     5401 gagttttgga ggaaatgggc ttagaaaccg ttggtacgca cggaacgact gctgcccttg
     5461 cattgcttaa tgacgcagtc aaaaagggtg gggtcatggc ttgtaaccaa gtcggtggct
     5521 tgtctggtgc cttcatccct gtttctgagg acgagggcat gattgcggcg gtgcaaaatg
     5581 gctcgctcaa ccttgaaaaa ttggaagcca tgacggccat ttgttctgta gggttggata
     5641 tgattgccat tccagaaaca acgcctgctg aaaccattgc ggctatgatt gcggatgagg
     5701 cagccattgg ggttatcaat cagaaaacaa ctgcggttcg cattattccg cttggaaaag
     5761 aaggggacat gattgaattt ggcggccttc tgggaacagc tcctgtcatg aaggtcaatc
     5821 aagcatcgtc tgtggacttt atcaatcgtg gtggacaaat tccagcaccg attcatagtt
     5881 ttaagaacta aaaaatgtag accgattgcc acaagcagtc ggttttttgc tatactatat
     5941 agtaagaaaa gacaaggaga atgctatggc agacgtaaga ttatatattt ctcgtcacgg
     6001 gaaaaccatg tttaatacca ttgggcgcgt gcagggctgg tgtgatacgc cgctgaccaa
     6061 ggctggtgag gaagggattc gtgaattggg cttggggctc aaggatgcag ggcttgattt
     6121 taaactagct gtatccagtg acttaggacg aactgttcaa actatgacca ttgcccagcg
     6181 tgagttggga attttaggga aaatccctta ttaccaagac aagcgtatcc gtgaatggtg
     6241 ttttggtagt tttgagggca tgtatgacgc tgagcttttc caaggtgtcc tgcctcgttt
     6301 gaaagggaca gtcgatgcga caggcatgtc ctttgcggaa attgcagcag gtatccaaga
     6361 ggcagacaca gcaggctggg cagaatcttg ggaggttttg agcaaccgta tcttaacggg
     6421 ttttgaatcc atcgcccagg atttggaaaa acaaggcgga ggcaatgccc ttgtggtcag
     6481 ccacggtatg accattgcaa cattggctca tttgttagag ccagaacgtg gggctaatat
     6541 tttcctagat aacggatcaa tcaccgttct caagtatgaa aatggcaagc ttttaattga
     6601 agcagttggg gatctatcct accgcaaacg tggggcagag ttgattgccc aaggcaaata
     6661 aatgtgaaat gaaaacatgt atgtcatacg ttttttctga tgtgtgacat acatgttttt
     6721 atatatgaaa tctaagttac atcattttaa ctctttcctg ctgaagataa agtaactcag
     6781 atagagatat agtatagtag ccataaagct aacactcatg gatagccttg aaatattttc
     6841 cccttgttgg agggaaagga atagaaaata atctgttggt aatttcatat ggagtagatt
     6901 actagtaatg aggttggaga tattgtcaaa aagaaggcta attaaactaa aggcaagata
     6961 ggcttgattg accttattag acttgacaaa tatcaggatg gacaacagag agatacagat
     7021 aaataaaggt gtttgagcaa tgaaactgtg tattatcact gcaacatcaa caggatcact
     7081 cagaagtaag ccaacactgc tactcaataa ataggctaga aggatataag cgagatctag
     7141 cacaagggtt gctaagactt tatagaaaaa tacccttttt cttgtttgtt gcttgataat
     7201 aatattattg atggtacggt aagtaaaatc ttctccaaag aataggttgg ctgggacgat
     7261 aaaaaatagt gtgataaatt gagaaagcaa ttgaataaga gagctagtga agtcttcgtt
     7321 gccgtgcaac aaaaaagcgg ataaaaagga cagtaaagcc aatattccca atgaaatcgt
     7381 taatctctgt tctttttgaa tcttaaataa atcagcttta aagtaatgta tcatgctctt
     7441 ctccttcttg aagcaatgtc agataataat cttcaaatga catttgttgt ttaaaaattt
     7501 ctttgacctc aatctcatgg tttatcaaga gatgaatgag gctcataaca ttcgtatctg
     7561 atgacagact gatataatcc ttatctattt tgtaagcgat gccctgctct tcaaggatag
     7621 taaaggtctt aggatggttg ttcgtttgta gatagagctg ttcttctagg gtttgttcaa
     7681 gttctcgttt tgagaattct ttgacgataa ggcccttatg cataataatg tatcgatcaa
     7741 ccaccatctc aagctctgat aagatatgac tggagataag gatggtcatc ccatattgat
     7801 tgcgaagatt taaaataatg cttctcattt ctttgattcc aacagggtca agaccattaa
     7861 tcggttcgtc taggattaaa aaatcagggt catctaggat agctaaggca atggctaaac
     7921 gttgacgcat tcccaatgaa aaatctttgg attttttctt aggatccaca tcctctaaac
     7981 cgacaattgc taatgtctct aagatttttt catccgcttg cttgagattt aattgcagag
     8041 ccatgtaacg aagattatcg atcgcagata aattgggttg tagagcaggg gattcaatta
     8101 aagctccaat acgacaatct tttgaactag taatggtacc tgaagtttgg gaaatgaggc
     8161 ctgataacat tctgagaagc gttgttttcc cagcaccatt ttcaccgact aagccacaga
     8221 tttcaccttt attaatcgtt aaagaaacct gatgaacggc cttttgtttc ccatattgct
     8281 ttgataattc ttgggtagat aagagagatg acataactac ctgcctttct ataaaaaatg
     8341 ataaatctat gatatactag attcaaaaat tggtaaattt cgttacataa atgtaacacg
     8401 tggagaatat aatgggaact tacggaaaaa tttttaaact cattcgtgaa tcaaaaaata
     8461 tgtctctaaa agaagtggct ggggatttcg tgacacctgc tcagttatca cgctttgaaa
     8521 atggaaagag caatctttct gtggatacat ttttttattg cttgagacgg atgaatgtgg
     8581 tagagggcga attttctacc ctctaccagt cctactttca gatagataat ttgttttcat
     8641 cagaggaatt tgtccgtgca gtttatagtc acaatgctca gtatatggat gatagaattt
     8701 tgtaccatca agcagagtat gatagggatg gacgtccgtt tgatagggtg atggttgcgg
     8761 cctttcatat ttttaagcaa caatggtgcg atcctgatta tgatgtttct gaggatgaaa
     8821 aagctatttt atcggactat ttaatgtcca tagatgattg ggggaaattt gaactctgga
     8881 ttttcggaaa ttgtagcaga ggtcttccta gcaagatgtt ggaagttctg ggaatggaag
     8941 ttctcaatcg aacaaagttt tatatggata tcacagaaaa cagagacaat gtctataagg
     9001 tactcatcaa tatttcgagt agtttgttag ataggggaga ggagattgct gcgattcgct
     9061 ttctcaaaat tcttgactct gtcaatatca tggaaaagaa tatgcgggat cgcttgctgc
     9121 ttaagctcct aaaagcacgc ttgtcctaca tgaatggaaa tgatggtggc ctagaaataa
     9181 tgaaggaatg cctgcatatt gccaaatttt tagattgcta tgatttaatc caccagatca
     9241 ataaggaaat tgagaaagtt acagatatgt aataaatttt aagtggctcg tcatttcctg
     9301 taagataata cttgtaagtt ttacaacttt acaaaacaat gtttagaaac tttagaaact
     9361 gtattcacaa gaaaagtgct tttagagaag agatggtttt tcgctaatca aggcagtttt
     9421 ttgagggaat actaactgta ttttgaaaaa aacgagcggt gagtagctga aaaactagcc
     9481 ttagttagaa gtgctgaact gtgaatacag gttcttaatt atcttggagg aaataatgat
     9541 gaagaaatta cttgttttag gtgctcttgc aatgttaggt tttgtaactt ttgggcttct
     9601 aacaactaat agcagttcag caagtgctga aacggagggc aaggtagttc tgtttggaaa
     9661 agtcgatttt tcagacgata aagaacaaga cacggatgag gaaagggaag aagtagcgac
     9721 ggagagcgat gatgctagct ggcttgaaaa ctttataacg acacataaaa caccattttc
     9781 taaaacaatc aagaaatgaa atcagggata ttctctgatt tttttcttga cttatatatc
     9841 gagatgcgat ataataaata catcgaagtt cgatgtatcg tagtgcgaaa ggagttttat
     9901 gaacgataag ttaaaacgcg tctatgtccc catgacagaa acagccttct atattctctt
     9961 tcatttgcag gaagaacggc atggctatga tattacccag aagaccaagg aagtgacggc
    10021 aggtcaggtc gtgattagcc ctgggaccat gtacggcacc ctgtcaaaaa tggagaagga
    10081 tggcttgatt gcctttgtca gagaagaaga caagcgaaag ttttaccaaa taacagcaac
    10141 aggtcgagaa attttagaca tagaattggc tcgaatcgaa cgtctttatc gaaatagtaa
    10201 gggggaaagt tatggagact aagacagaaa ttcatttttt cttcttgcct gattttgaaa
    10261 aggaagaaca ttatttggca gagcaacatc gtcagggatg gaaatttcaa aaaaataaat
    10321 ttggcttttt ctatatcttt gagaaatgtc agcctgaaga agttatctat caactggatt
    10381 ttaagcccaa tggtcaggat aaggaggaat acctccagat gtttgcagat tatggctggg
    10441 aatatgtcgg tgattgcaat catttttcat actttagaaa gaatgagagt ttgggagaag
    10501 ttgagcttta tagtgaccgt cagagtaagt tcgagatgat tgacagaatt attaccagac
    10561 agtttctact ggtcagttcc ttgtttgttt tctttatctt gctattttat gtcttgaagt
    10621 tgcctgctgt tatgattggt atggcagttg ctgacttacc agtgttactc tattgttcta
    10681 tcggcttgat gcgtttgcgt cagaagtata aggagattgg aaaatgacaa aacaaaaacg
    10741 tatagcaacc ctcatcaatg ctcttgcaat ctttgcagtg atttttctaa atcggaatag
    10801 ttttatcagc ttgattgcta ttacgctgat aatgatttta aatttttgga atttaattga
    10861 atatgaagga agaaaagtaa ttcaaaaata tttgtggaaa agaactttta tcatttggac
    10921 aatccaccat tctaggtcct cagactgatt tttagcactc tagataaaag agtgctaatt
    10981 ttttggtttt ttctcttgac aatccatttt ttcggcgtat aatgagagta tatagtttag
    11041 cactcgccat acgagagtgc taaatctaaa aatcaatcgt gttcatgaaa ggagcggaga
    11101 tgattaccca acgtcaaaat gatattttga atctgattgt tgaattattt acgcgtcatc
    11161 atgagccagt tggttctaag accttacagg atatgattgc ttctagctct gctaccattc
    11221 gcaatgacat ggccaagcta gagcaactgg gcttgctaga aaaggctcac acttcaagtg
    11281 gtcggatgcc cagtcgggct ggttttcaat attttgtcaa ccactcgctt aatctggaac
    11341 atatcgatga agaagatgtt tatcaagtgg ttaaggcttt cgactttgaa gctttcaagc
    11401 tggaagacat cttggagcgg gcaagtcagg tcctagcaga tttgacaggc tacacctcgg
    11461 tcattttaga tgtggagccg accagtcagc agctgacttc ctttgacatc gtgcagctca
    11521 gtagccacga tgctctggca gtcttgactc tggaccagtc caaacctgtc accgtccagt
    11581 ttgccattcc caagaacttt ttggccaggg acttggaggt gctcaaacgc ctggtggatg
    11641 aacgcttcgt tggacagaca gtcttgtcca ttcactacaa gctgcggacg gagattcccc
    11701 aagtagtgca gcgttacttt accacgacgg acaatatctt ggacctgatg gactacatct
    11761 tttccaatct tttccaagaa tcggtcttta tcaatggaaa agttgcctcg ctgacttacg
    11821 gcaatctagc tacctaccaa ctcttggata gtccgcaggt gcttgctagt gagttgcggc
    11881 agggcttggc tcccaaccag cagacaagca tttctgtggc agaacataga gagcctgctc
    11941 ttgcagatgt gactatcatt catcatcgct ttccaatccc ctatcggggc atggctcaga
    12001 tgagtttgct tggtcctatt gatatggact atcgtaggca gatgagtttg atcaacatca
    12061 tcagccgcgt cctatttatg aaattaaccg attactaccg atatctgagc agtaatcatt
    12121 atgaggtaaa ttaagatgtg agggcgtgaa ggaaacgatt gaaaaatagg agattgacgc
    12181 ggtgtcatca gacacaagga aatctatcat ttttctgagt ttctagcccg aactcaattt
    12241 attatttgtc aaagtaataa tagaaaatga agaggtgaac aacttgtcag aagaaatcaa
    12301 aaacgaagaa atcgtagaag aggttgaaac aacagaagaa gttgtggaga cacctgaaaa
    12361 atcagaattg gatttggcaa atgagcgtgc ggaggaattt gaaaacaagt accttcgtgc
    12421 ccacgctgaa atgcaaaata tccaacgccg tgccaacgag gaacgccaaa ccattcagcg
    12481 ttaccgttcg caagacttgg ccaagaaaat cttgccaagc ttggataact tggaacgtgc
    12541 ccttcaagtc gaaggtctga cagaagatgt caaaaaaggc ttggaaatgg ttcaagaaag
    12601 cttgattcaa gcccttaaag aagaaggggt ggaggaagtc gcaacagatg tttttgaccc
    12661 aaatcttcac atggccattc aaacagttcc agctacagac gattgcccag cagaacacat
    12721 tgcacaagtc ttccaaaaag gctacaagct acatgaacgc ttgttgagac ctgctatggt
    12781 agtcgtatca gagtagtcgc tctgcttttt ccccaaaaaa gaaacttgac cgaaatgtca
    12841 ttaaactagg cggtaagtca gaaatctttg atttcgttga cgcccccgca aggctgacta
    12901 gaattttcat agcctatcag gcgtagaaag ttcagtcagc tactgcgaaa atattatgta
    12961 gggtaaaacc taactaatga tgtagtagaa aaactgaaaa agaggtattt ataatgagca
    13021 aaattatcgg tattgactta ggtacaacaa actcagcagt tgcagttctt gaaggaactg
    13081 aatcaaaaat catcgcaaac ccagaaggaa accgcacaac accgtctgtt gtgtctttca
    13141 aaaatggtga aatcatcgtt ggtgacgcgg caaaacgcca agcagtaacc aacccagata
    13201 ccattatctc aatcaaatca aaaatgggaa cttctgaaaa agtttcagca aacggtaaag
    13261 aatacacacc gcaagaaatc tcagctatga tccttcaata cttgaaaggt tatgcggaag
    13321 aataccttgg tgaaaaagta accaaagctg ttatcacagt tcctgcttac ttcaatgatg
    13381 cacagcgtca agcaaccaaa gacgctggta aaatcgctgg tcttgaagta gaacgtatcg
    13441 tcaacgaacc aactgcagca gcccttgctt atggtttgga caagactgac aaagatgaaa
    13501 aaatcttggt attcgacctt ggtggaggta ctttcgacgt atctatcctt gagctcggtg
    13561 acggtgtctt tgacgtactt gcaacagcag gtgataacaa gctcggtggt gacgactttg
    13621 accaaaagat tatcgaccac atggtagcag aattcaagaa agaaaatggc atcgacttgt
    13681 cagctgacaa gatggctctt caacgtttga aagatgcagc tgaaaaagcg aagaaagact
    13741 tgtcaggcgt gacatcaact caaatcagct tgccgttcat cactgcaggt gcagctggtc
    13801 cgcttcactt ggaaatgaca ttgactcgtg ctaaattcga cgaattgact tacgaccttg
    13861 tagaacgtac aaaagttcct gttcgtcaag ccctttcaga tgcaggcatt agcttgtcag
    13921 aaattgacga agttatcctt gtcggcggtt caacacgtat cccagccgtt gtggaagctg
    13981 taaaagctga aactggtaaa gagccaaaca aatctgtcaa ccctgatgaa gttgttgcca
    14041 tgggtgcggc aatccaaggt ggtgtcatca ctggtgatgt caaagacgtt gtacttcttg
    14101 acgtaacacc attgtcactt ggtatcgaaa caatgggtgg tgtgtttaca aaactcattg
    14161 accgtaacac aacaatccca acttctaagt cacaagtctt ctcaactgcg gcggacaacc
    14221 agccagctgt tgatatccac gtgcttcaag gtgagcgtcc aatggcagca gacaacaaaa
    14281 ctcttggtcg tttccaattg acagacatcc ctgcagcacc tcgtggtatt ccacaaatcg
    14341 aagtaacatt cgacatcgac aagaacggta tcgtttctgt aaaagccaaa gaccttggta
    14401 cacaaaaaga acaaaccatc gttatccagt ctaactcagg tttgacagat gaagaaatcg
    14461 accgcatgat gaaagatgca gaagcaaacg ctgaagcaga taagaaacgt aaggaagaag
    14521 tggaccttcg taatgatgtt gaccaagcaa tctttgctac tgagaaaact ctgaaagaaa
    14581 ctgaaagcaa aggcttcgac gcagaacgtg atcaagccca agcagctctt gacgagttga
    14641 aagcagccca agaagctaac aacttggacg acatgaaagc caaacttgaa aacctcaacg
    14701 aaaaagctca agctcttgca gtgaaactct acgagcaagc cgcagcagca caacaagcag
    14761 ccgcaggtca agaaggtccc caaacagcca acaacgcagg cgatgatgtg gtagacggcg
    14821 agtttacgga aaagtaaaat agtcctgtgg actattttag cccgtgagcc agaaatgaga
    14881 aatgagcggt tggactggga gaccttttta tcctgacttc ttgaaaatta gtaggaagtc
    14941 ggttaaggtt cttagctcga actcagttct tatcctatcg agaatgggaa ttcagaaacg
    15001 agcagttggt ccgggtgatt gttttatccc gagccacgaa attcttcact aggcttcgtt
    15061 aactctcctc gatgaactct agttcaatct tcgtccagtt gccttgccta gtcttgaatt
    15121 tctttgagta tctagaattt ggaaagttat cttttttccg aagttcttag ctcgaactca
    15181 gttctcatct ttccgagaat agaaatcatt cgtcaataaa atttccaatc acagaggttg
    15241 caacccagcc tctgtttttg ggtaaatacc cttctaactt ttacagaaag gaatacaagt
    15301 tcgtttattc gaacacaggc taaaaaccta gtgaaaaaga taaccctcct agtgtgtttt
    15361 acacactgcg tcagtttcct attttcactt ttccaaaacc atgatagaaa ggaaagtgtt
    15421 tgtaatcgaa cacgggctac ggactgtgcc aaaaagatag tttttcctag gacggtgagg
    15481 tcctgcgtca aaactcctat tttggctgtg tccgctttac gcccttagta tcttatatga
    15541 acaatactga attttacgat cgtcttggtg tttctaagaa tgcttctcca gacgagatta
    15601 agaaggctta tcggaagctt tcgaaaaaat accatccaga tattaataag gatccgggtg
    15661 cggaggataa atacaaagag gtccaggagg cttatgaaac cttgagtgac ccgcaaaaac
    15721 ggtctgccta tgaccaattt ggtccagctg gtgtcaatgg aggcttcggc ggaggagcag
    15781 gtggatttgg tggtttcgac ggagcaggtt tcggtggctt tgaagacatt ttctctagtt
    15841 tctttggtgg aggtggtgcg acgcgcaatc ctaatgcacc tcgtcaaggg gatgacttgc
    15901 aatatgcggt caatttgaaa tttgaagaag cgatttttgg tgtggaacgt gaagtttcct
    15961 ataaccgtga ggcaacttgt cgaacctgta caggttcagg tgcgaaacct ggaacaagtc
    16021 cggtaacctg tgggcgttgt catggttctg gagttatcaa tgtggacacc caaactccac
    16081 ttggaaccat gcgtcgtcaa atgacctgtg atgtctgtca tggtcgcgga aaacaaatta
    16141 aagatccatg tacaacctgt catggaactg gtcatgaaaa acaagcccat acggtaacgg
    16201 ttaaggtacc agctggtgtg gaaaccggtc aacgtattcg cttggcaggt caaggtgaag
    16261 ctggtttcaa tggaggtcct tacggagatc tctacgttgt tattcaggtc caaccttcag
    16321 acaagtttga acgtgaaggg accactattc actataaact gaacctcaac tttgttcaag
    16381 cagcacttgg agatacagtt catgtgccaa cggttcacgg tgatgtggat atggtcattc
    16441 ctgaaggaac ccagactggt aagaccttcc gactcaaagg aaaaggtgca ccaagtgttc
    16501 gtggtggagt catcggtgac caatatgtga cggtaaatat cgtcactccg acaggtttga
    16561 atgaccgcca aaaagcagct cttaaagaat tcgcggcagc aggaaatatt gatgtcaaac
    16621 cccataaaaa aggatttttt gacaaggtaa aagacgcctt tgatgagctg taaattgctt
    16681 ttatcggcat actcaaaatc agtaagcatg gcttgctgat tttttacttt catgataagc
    16741 taataaaaaa tacaaggaga tgcctatgtg gaaagatgcg acggaaatgg caatggccgt
    16801 gcgagcaggt caggtctctg ctaaagaatt ggtagcggaa gctattgaac gaatagaaga
    16861 ggaaaatcct gctctcaatg ctgtcgtcag caagcaatac gagcaagccc tgcaagaagc
    16921 agaaacgggg aattttcaag acaaggcctt tggtggtgtc cctctcttat tgaaagactt
    16981 ggggcaaaat gaagcgggac aattgtcaac agcgggctct cgtctattcc agaattaccg
    17041 agcggggcat acagataaat tggtacaggc tttcaaggat atgggtttca tcatcttggg
    17101 ccggaccaat acgccagagt ttggttttaa gaacatattt gactccagcc tacatgggcc
    17161 agtcaagtcg ccgctagatt tgagccgaaa tgcaggtggg tccagtggtg gtgcagcagc
    17221 ggcagtagct tctggtatgg tacctttggc agcagcttca gatggtggcg gttctatccg
    17281 tattcccgcc tccttcaatg gtttgattgg gctcaagccc agtcggggtc gcattcctgt
    17341 tggaccaatt tcttatcgag gttggcaggg agcttccagc aactttgctc tgactaagtc
    17401 agtccgcgat accaaacacc ttctttatca cttgcagatc tatcaggtgg aagctccctt
    17461 tcctctggct ctcctgtctg agcagagtct ccttgcaccg caagataaac ctctgaaaat
    17521 ggcctacagt ctggaatctc cgattggcag tccagtttcc gctgatgcca aacaggctgt
    17581 cttgtccctt ctgcctcagt tagaagcact ggggcaccag attaccgagc tagcaagtcc
    17641 tattttggac ggccttgaag tcatgcgggc ctattacctg atgaacagtg ttgaaacagc
    17701 tcagatgttt gacgagattg aggcggggct tggtcgtccc atgacagctg atgacatgga
    17761 ggtcatgacc tgggcgatct agccctaacg ccctttacgc agcaggccaa tatttgtggt
    17821 cagccagcca tttctctgcc aactcatgtg cgggctgacg gcttaccaat cggcatccaa
    17881 ttgacagcag ccaagggacg agaggattta ctcttgcagt tggcagacca gttagaacaa
    17941 gctggacttc ttcacttata acttctccat tttttcagtt caattatttg ccaaaacgtt
    18001 ttcaaaatgt taacctataa aagttggtgg gataacacta aaaaagtaaa aaggaagcta
    18061 tggcttccaa aggagattgt tttatgttga aaaaagtttt ggcctcagct ctcttggttt
    18121 ctactttgac cttggcagca tgttctagct caagttcgaa tactagcagt tcttctgctg
    18181 aacagactca atgggaaaaa gtacaagaag caggtgtctt gaaggttgct acaccaggta
    18241 ccctcttccc aacttcctac tacaacgatt ccaaagaatt ggtcggttat gaaattgaca
    18301 tgatcaatga aattggtaaa cgtttgaaat tggaaatcga ataccaagaa atcggtgtag
    18361 cagaagcctt tacagctgtg gacagtggaa aggtcgatat ttctgttaac aactttgaca
    18421 tgacagaagc acgtaaggaa aagtacaact tctctatccc ttacaagtat tcagttggtg
    18481 gctacatcgt tcttgaagat ggttcatcag gaatcgaagc agctgacttg agtgactgga
    18541 ctggtaaaaa agctggtggt ggcgcaggta ctcagtatat gaagattgct gaaaagcaag
    18601 gtgcagagcc agttatctat gacaacgtaa ccaacgatgt ttatttgcgt gacgtatcaa
    18661 caggccgtac agacttcatt ccaaacgact actacacaca agtcatggcc atcaaatggg
    18721 caaatgctaa cttcccagac atcaaggtaa aaatgggcga tgcaaaatac aacccaacag
    18781 aacaagggat tgttatgagc aaggccgaca caagcttgaa agaaaaaatt gacgaagctc
    18841 ttgaagcgat gaaagctgac ggaactttga aggcaatttc tgagaaatac tacgcaggac
    18901 aagacttgac agtccctgtt gaaaatgcag ataaattgcc agttatcgaa gttgaataag
    18961 acctcgtaac aaattaaatg catcttgtat agactttgtc agtcacagac tggcaaggtc
    19021 tatctggttt ttaggaaaga ggagaagata tggatattaa ctggcaggca gtttttaatg
    19081 cagatattgc aagagaggct attccacaga ttttggaggg acttccctac actctatcac
    19141 tatccctaat tggttttgcc ttgggaactt tcggtggatt ttttgtagcc ctcatgcgga
    19201 tgtctaagtt tggccccttg cgttggttgg ctatggccca tatttctctc atgcgtggaa
    19261 ttcccctcat ggttcttctc tttttcatct attttggatt gcctttcatg gggctacaac
    19321 tagatgctat ctccgcatct attatcgcct ttacctccat gtctagtgcc tacatttctg
    19381 aaattattcg tgcatctctt tcggccattg ataagggaca atgggaggca gcgcgttccc
    19441 tgggcttgcg gaccaatgtg atttacagaa aaatcattat tccccaagcc tttcggattg
    19501 cccttccacc actgagcaat gttctcttgg atatggtgaa aagtacgtcc ttgaccgcta
    19561 tgattactgt accagagatt ttcaataagg caaaaatagt cggcggagcc aagtcggatt
    19621 atatgacggt gtatatctgt gtagccttga tttactgggt tatctgtacc ctttatgcat
    19681 ttggtcaatt acaactagaa aaacgcttgg ctacctatta aaaaaaggtt ggcatatcct
    19741 tccgattaaa atttgaacaa tcaaaaatag cttagaatta acgtctcgaa tgacgtggtc
    19801 ctaagctatt tttttatttt aaagactgtt t
//
LOCUS       DATUCG010000031        19250 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.032, whole genome shotgun sequence.
ACCESSION   DATUCG010000031 DATUCG010000000
VERSION     DATUCG010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19250
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.032"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(82..792)
                     /locus_tag="VCA20_000871"
     CDS             complement(82..792)
                     /locus_tag="VCA20_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1834718.1"
                     /translation="MAMLEVKNLSVNYGVIEAVKDVSFEVNEGEVVTLIGANGAGKTS
                     ILRTISGLVRPSAGTISFLGNEIQKVPARKIVADGLSQVPEGRHVFAGLTVMENLEMG
                     AFLRNNREENQANLKKIFARFPRLEERKNQDAATLSGGEQQMLAMGRALMSQPKLLLL
                     DEPSMGLAPIFIQEIFDIIQDIQKQGTTVLLIEQNANKALAIADRGYVLETGKVVLSG
                     TGKELLASEEVKKAYLGG"
     gene            complement(792..1556)
                     /locus_tag="VCA20_000872"
     CDS             complement(792..1556)
                     /locus_tag="VCA20_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1834719.1"
                     /translation="MALLEVKDLTKNFGGLTAVGDVTMELHEGELVGLIGPNGAGKTT
                     LFNLLTGVYEPSEGTISLAGTILNGKAPSKIASLGLGRTFQNIRLFKNMTVLENVLIG
                     LGNHGKSEVFASFFRLPAFYKNEEALKTKAIELLKIFDLDGDADTLAKNLPYGQQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTQLIRKIKEEFGITIILIEHDMSLVMEV
                     TERIYVLEYGRLIAHGTPEEIRNNKRVIEAYLGGEA"
     gene            complement(1556..2503)
                     /locus_tag="VCA20_000873"
     CDS             complement(1556..2503)
                     /locus_tag="VCA20_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002914606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEP1834720.1"
                     /translation="MKQNLKVNAIWLAILLAGFGLIQGLVSAGILNFYYIQIVQQIGI
                     QIILAVGLNLIVGFSGQFSLGHAGFMAIGAYAVGILGKMMPTYGGFAIALIVGMLVAG
                     GVALLVGLPTLRLKGDYLAIATLGVAEIIRILIINGGSVTNGAAGIMSIPLFTSWPLV
                     YIFVIITTLVTVNFLRSPMGRATISVREDEIAAESVGVNPTFVKVSAFVFGAMAAAIA
                     GGLHAGYVGTIVPKDFAFMTSVNVLIIIVLGGLGSITGTFVAAIVLGILNVFLKSYSD
                     ISMIIYSLALILLMIFRPGGLLGTKEFSVAALLKKKEVK"
     gene            complement(2506..3393)
                     /locus_tag="VCA20_000874"
     CDS             complement(2506..3393)
                     /locus_tag="VCA20_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEP1834721.1"
                     /translation="MLQQLVNGLILGSVYALLALGYTMVYGIIKLINFAHGDLYMMGA
                     FMGYFLLNNLTFIADGGTRFFVALILAMVGTAILGVVIEFLAYRPLRNSTRIAALITA
                     IGVSFFLEYTMIKFFTADVKAFPQAIEAVTFNIGPVSVTNIQLIILGVSLALMVALQL
                     IVKRTKMGKAMRAVSVDSDAAQLMGINVNRTISFTFALGSALAGAAGVLLGLYYNQIE
                     PLMGMTPGLKAFVAAVLGGIGIIPGAALGGFVIGIIETFTYVIEFDTFRDAIVYTVLI
                     IILLVRPSGILGKNVKEKV"
     gene            complement(3609..4778)
                     /locus_tag="VCA20_000875"
     CDS             complement(3609..4778)
                     /locus_tag="VCA20_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEP1834722.1"
                     /translation="MKTRKFAVALATFASAALLAACGNVSTTNTSAAGSSVGDTFKIG
                     YNLELSGAVSSYGQTEEKGANLAVKEINAEGGIDGKKIEVITKDNKSETAEAATVATS
                     LASEGANVVIGPATSGASAASIPALTSAGVPMITPSGTQTNLVVNDKGEVQEYFFRTT
                     FTDGYQGQIMAKYATESLLAKKVVLYFDNSSDYGKGVAEAFKKAYTGEIVSEITFASG
                     DKDFQAALTKLKDKEFDAIIMPGYYNETGTIVKQARGLGIDKPILGSDGFDSPQFTEL
                     ATASAASNVYYLSGFVTSASEKAKAFYDAYVKEYNEEPSMFSALAYDSVYMAAEAAKG
                     AADSAAVKANLAALKDFKGVTGTMSVDKAHNVIKSVYVVSLTNGEESSVDTISID"
     gene            complement(4911..5183)
                     /locus_tag="VCA20_000876"
     CDS             complement(4911..5183)
                     /locus_tag="VCA20_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEP1834723.1"
                     /translation="MFEKKNRTCLTVYLHYNRDARKLSQYGDIVYHSKRLRYVLVYMD
                     QELVEATILKLKKERFVKKVVPSYIKELDQNFVGNLWRDEEPSVVG"
     gene            complement(5310..5900)
                     /locus_tag="VCA20_000877"
     CDS             complement(5310..5900)
                     /locus_tag="VCA20_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEP1834724.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSIIA
                     QLLFLDAQDPTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGTAASMGTIIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRNKLEKILADNSGK
                     TVKQIHKDAERDYWMSAEETLAYGFIDQIMDNTKVK"
     gene            complement(6017..6646)
                     /gene="upp"
                     /locus_tag="VCA20_000878"
     CDS             complement(6017..6646)
                     /gene="upp"
                     /locus_tag="VCA20_000878"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEP1834725.1"
                     /translation="MGKFQVISHPLIQHKLSILRRTSTSTKDFRELVNEIAMLMGYEV
                     LRDLPLEDVEIETPITKTVQKQIAGKKLAIVPILRAGVGMVDGLLSLVPAAKVGHIGM
                     YRDEETLQPVEYLVKLPEDIDQRHIFVVDPMLATGGSAILAVDSLKKRGASNIKFVAL
                     VSAPEGVKALQDAHPDIDIYTAALDEKLNEKGYIVPGLGDAGDRLFGTK"
     gene            complement(6726..8342)
                     /locus_tag="VCA20_000879"
     CDS             complement(6726..8342)
                     /locus_tag="VCA20_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEP1834726.1"
                     /translation="MPEIQKTDWWKKSVIYQIYPRSFQDSNGDGVGDIRGIISRLDYL
                     HELGIDAIWLSPVYQSPMDDNGYDISDYQGIAPEFGTMEDMEELIAEGHKRNIKIIMD
                     LVLNHTSDEHFWFQEALKGPDNPYYDYYVWADEPNELRSTFSGSAWEYVPHLNKYYLH
                     QFSVKQPDLNWKNPALKQEIWDMINFWIEKGVGGFRLDVIDLIGKEPEKLITADGPTL
                     HPLIQELNEKTFGAYDLVTVGETWSANPENSQLYSHPDRKEFSMIFQFEHVGLDQQEG
                     KEKWDLAPLDPARLHNVLSKWQTELVGKGWNSLFWNNHDLPRAISRFGDDRPAFRELS
                     GKMLAIYLHFMSGTPYIYQGEEIGMINAPITDISQADDIETRRMYAERIENGFTKEEL
                     ITSINAKGRDNARRPMQWTAEEGAGFSTGQAWLALGDTVKDINVEKALADKQSLFYTY
                     QKLIALRKEYPCMSEGKYEVMETGNSSVMAYRKYDEQDDFIILVNFSSQEQAYTIAVE
                     EYSIFMNNYENEEAINKGMLRPFEAIVFKK"
     gene            complement(8415..9863)
                     /gene="gtfA"
                     /locus_tag="VCA20_000880"
     CDS             complement(8415..9863)
                     /gene="gtfA"
                     /locus_tag="VCA20_000880"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HEP1834727.1"
                     /translation="MKIKNQAMLITYSDSLGKNLKDLKKVLEGPLKDVVGGIHILPFF
                     PSSGDRGFAPMDYTKVDPAFGDWSDIEALSKDYYLMFDFMINHISAKSPYFLDFLEKK
                     DESAYADLFIRYKNFWPNGEPTQEDVDLIYKRKPRAPYRIATFADGSEEKVWCTFDEQ
                     QIDLDVTTETTRRFIQENLEQLAEHGASIIRLDAFAYANKKIGTNCFFVEPDIWEMLH
                     FPRQLLAPKDVEILPEIHEHYTIQQKIAEQDYYVYDFALPMLVLHALYSGHVNRLVHW
                     MEICPRKQFTTLDTHDGIGVVDVKDLLTDEETEETREALYAQGANVKKIYSTEAYNNL
                     DIYQINCTYYSALGNNDQAYLLARVLQCFAPGIPQIYYVGLLAGENDIELLESSKEGR
                     NINRHYYDLEEIEQEVQRPVVQSLFKLLKFRNTSPAFDGEFSVKMLDETSMEIFWNNQ
                     DAGVSARLTANLKEKSFEIVEIEEGKITTIEL"
     gene            complement(9933..10763)
                     /locus_tag="VCA20_000881"
     CDS             complement(9933..10763)
                     /locus_tag="VCA20_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEP1834728.1"
                     /translation="MNTQKQKNWWVYLVLFSGILFMFIPLLVTIISSFKPTKEITSNF
                     FGLPENFTLNNYERLFNDGIVQYFGNSALITIVAVGLILLVIPMAAFAVARQMKRQTV
                     FNFIYFFLIIGIFVPFQVIMLPMTKLMSSLGLNNIVGLIILYLTYAVPQALFLYVGYI
                     KTIVPEEMDEAAAIDGCDKFTMYWKIIFPLMKPMHATVLIINALWVWNDFLLPLLVLN
                     RDQSMWTLPLFQYNYQGMYFSDYGPSFASYVVGIIPILLVYLVFQKHIISGMTSGSVK
                     "
     gene            complement(10774..11646)
                     /locus_tag="VCA20_000882"
     CDS             complement(10774..11646)
                     /locus_tag="VCA20_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEP1834729.1"
                     /translation="MNQKGFIAKYWPYLFVAIPIGLQLIFFFYPLFTGIYYSLTDWNG
                     LTSDFKLIGIQNYLDILKNPDFYTSMTFTIIFTIGLVIGEIVIGIWLASLLNRKIKAV
                     GFFRTWYFFPAVLSTVTLGLIFVQLFNYGFTQIGEILHIDWLMENLLVQENTVIPAVL
                     FVALWQGLAMPVIIFLSGLQSIPEDVKEAAAIDGASRSQQFFNIELPFLLPSISMVFI
                     LAMKSGLTAFDLIFALTSGGPDGKTESLGLLVYNYAFVDNKFSYANALAVVLFIFIIV
                     ISLIQMRISKKFEI"
     gene            complement(11781..13016)
                     /locus_tag="VCA20_000883"
     CDS             complement(11781..13016)
                     /locus_tag="VCA20_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEP1834730.1"
                     /translation="MKMQTFLKCASVCAFASFLVACGNASSSDKVEIEYFSQKPEMQA
                     TLQEIINDFEKENPTIDVKFSNVPDAGTVLKTRMANNEAPDVINIYPQNADFKAYAAD
                     GRFLEIGDDAGLSHLKDGAVTPYLVNEKNYTLPLTANAYGIYYNKDKFKELGLEVPTT
                     YAEFVALVDKIKSDGSAAPFALSLNDAWSLNGYHQLAWVTVAGGFDGAEDILIRSAKG
                     AIQDDATTKAVLERLQLLTDNGQKGATGALYADAVAAFAAGDALMLPQGTWAATAVNQ
                     QEPEFEYGMFTFPGDKEGGDYTIGAADLALSISADTEHPEESKKFLEYLSRPEVIQKY
                     YDVDGSPTSVEGVDTEGKFEETAGVTQYAFTDKHVVWLQSEWESEEEFWNITVEMVKN
                     PNSAELVKKLNAFFDPMKK"
     gene            complement(13029..15203)
                     /locus_tag="VCA20_000884"
     CDS             complement(13029..15203)
                     /locus_tag="VCA20_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEP1834731.1"
                     /translation="MNVIEIYNEKQIFHLKTREFSYIIQVLETGDLVHRYFGKKIEKF
                     SDGNKITYLDRSFSPSPITGDRTYSLDVLPLEYSSNGLGDFRTSALDVRNEFGVTLDL
                     KYKEYRLYKGKKELRGLPASFGNQEEVESLEIDLYDQLTDITVTLQYSVFEEASYLAR
                     SATIQTGKYPCKLEKVLSATLDFPHQDFIVHSLAGRYAYEKEWTQTPLTKGQYSIGSI
                     RGASSHSRTPFLALASPDASEDKGDVYAAHLVYSGNFTAFVETTAMETSRLGLGLESH
                     YFSWQLDKDDRFQTPEVLLSYTDKGFTGMTQNSHHFITKHLIRSSFVNKPRPILINNW
                     EATYFEFTEEKILQLAQVASRAGIELFVLDDGWFGKRNNDESSLGDWKVNLDKLPNGL
                     NGLAERINELGMKFGLWFEPEMISIDSDLYREHPDWAIRTEGRLPIYSREQLVLDLTK
                     QEVCDYIIDSVSSILESANISYVKWDMNRNITNIPEGLANDQRFEFHHRYMLGLYRVL
                     DHLTKRFPDILFESCAGGGGRNDLGIMYYMPQAWASDDTDAIERLSIQEGTSFIYPTS
                     SIGAHVSAVPNHQVGRITPLATRGNVAMMGGAFGYELDLTKLSEKELDEISQQIETYH
                     SIRETIQFGQLYRLKKTTNTWAANYVSQDKNQAVFTFVKILAKPEAPLLHVRLKGLDP
                     DALYECPQLGETFYGDELMNIGLTMPHVQKDYFSVQYIFNKI"
     gene            15308..16147
                     /locus_tag="VCA20_000885"
     CDS             15308..16147
                     /locus_tag="VCA20_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEP1834732.1"
                     /translation="MNILNIYNELDSHNFDLNVDHYGAEQCDKGYSFGPTIRDNFVLH
                     FITKGRGKILVDGKTSYLAAGDLFILPKDVSIFYQADENEPWTYIWVGFSGSRAKDLL
                     NQSQLLENYYLHSNLESPILGYMHQINHVQMHPIPSITELVLIGYLNQLLAALIEEFP
                     SETLIKPETQTKYYVQQAIKMIHNHYAHPIKVSEIADFLALSRSYLYKIFKQETGYSI
                     KDYILQVKMNRSCQLLEDASRSITEIAYSVGYQDPLTFSAAFKNYFHMSPTEFRKTQK
                     NKD"
     gene            complement(16179..17339)
                     /locus_tag="VCA20_000886"
     CDS             complement(16179..17339)
                     /locus_tag="VCA20_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEP1834733.1"
                     /translation="MTQYNFIDSPNRLSQHAEKWRKVETDKELLPLWIADMDFEPLPE
                     IRQVIRDYADHHVFGYPYASDSLYQSIIDWEDRQHGYKIERESVLLIEGVVPALSVAI
                     QALTEEGDAVLINTPVYPPFARTVKLNNRQLVTNSLVDVDGIFRLNLDQLEKDIVENQ
                     VKLYIFCNPHNPGGRVWTREEVLAIGRLCQKHGVILVSDEIHQDLTLYGHKHYSFNTV
                     DESFKEFSIILSSATKTFNIAGTKNSFAIIENPSIRKTFAKRQLINNQHEIPTIGLLT
                     TEAAFTYGDEWLKELKVILERNIDFVEEYLTTHTRIKVMKPQGTYLIWLDFSAYELEH
                     AELFDILHCKAKLILNDGLTFGKEGKHHARLNVAAPFNMIEEACQRLGKVFG"
     gene            complement(17343..18434)
                     /locus_tag="VCA20_000887"
     CDS             complement(17343..18434)
                     /locus_tag="VCA20_000887"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEP1834734.1"
                     /translation="MTDYKIDTILAHTGINSDERTGALISPIHLSTTYQHPEFGQSTG
                     YDYTRTKNPTRASLETTLAAIEKADYALATSSGMAALVLLFNGFPVGSQVVAARDLYG
                     GSFRWFNEQESIGRFQFTYANTEEELIAAITEETDYVYLETPTNPLMVEFDIAKVSAI
                     AHAKGAKVIVDNTFYSPIYQNPLVLGADVVLHSATKYLSGHNDVLAGALMTNDQDLYD
                     KLFYDQNTSGPTLSPLDSYLLMRGLKTLSLRMERATQNAQKIVAYLEKSPAVKQVYYT
                     GKGGMISVKVVDESRIPHILNTLKVFTFAESLGGVESLITYPATQTHADIPAETRHSY
                     GLTDDLLRLSIGIEDAEDLIVDLKTALEG"
     gene            18908..19162
                     /locus_tag="VCA20_000888"
     CDS             18908..19162
                     /locus_tag="VCA20_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834735.1"
                     /translation="MSKKFNKAFYRTWEFYLSLACILQVISTAIHGPFWFIHFLKLGV
                     GLYAGYLAFFKSRSWIKKTWQIYLVIGLIASWTIFYFFVL"
BASE COUNT         5850 a         4122 c         3519 g         5759 t
ORIGIN      
        1 aaggtccccc ggaccttgat ataaagaaaa acgcctcccg gcgtttccta tttccaacct
       61 aaaaaggtcc cccggacctt tttagccacc cagatatgct tttttgactt cttctgaggc
      121 gaggagttct tttcctgttc ctgagaggac aacttttcct gtttctagaa catagccacg
      181 atcggcaata gcgagagctt tgttggcatt ttgctcaatc aagaggacag ttgttccttg
      241 tttttgaata tcttggatga tatcaaaaat ttcttggata aagatcggag ccaagcccat
      301 tgatggttca tcaaggagca agagctttgg ttggctcatt agggcacgtc ccatggccaa
      361 catctgttgc tccccacctg aaagggtagc tgcatcctgg tttttacgtt cttccaaacg
      421 tggaaaacga gcaaagattt ttttcaagtt tgcttggttt tcttcacgat tgttacgaag
      481 gaaggcaccc atttccaagt tttccataac agtcaagcct gcaaagacat gtcgaccttc
      541 tggaacttgt gacaaaccgt ctgcaacgat tttacgagca ggaacttttt gaatctcatt
      601 tccaaggaaa gaaatcgttc cagctgatgg acgaacaagt cctgaaatag tacgaaggat
      661 agaggtttta ccagcaccat tagcgccgat aagggttaca acttcacctt cattaacctc
      721 aaagctaaca tctttaacag cttcgataac accgtagtta acggaaagat tttttacttc
      781 taacattgcc attaagcttc acctccaagg taggcttcaa ttacacgctt gttattacga
      841 atttcttctg gtgtaccatg ggcaatcaaa cgaccgtact ccaatacgta tatccgctcc
      901 gttacttcca taaccaagct catatcgtgt tcgatcaaga tgatagtaat gccaaattct
      961 tctttgattt tacggatgag ttgggtcaat tctgccgttt cttgtgggtt cataccagcc
     1021 gctggctcat ccaagaaaag aatttttggt tcagtcgcaa gggcacgaac gatttccaaa
     1081 cgtcgttgtt gaccgtaagg aaggttttta gctaatgtat ctgcatctcc atccaaatcg
     1141 aagattttca aaagttcaat cgcttttgtt ttcaacgctt cttcattctt atagaatgct
     1201 ggaagacgga agaaacttgc aaatacttct gatttaccat ggttgccaag accaatcaaa
     1261 acattttcca aaacggtcat atttttgaac aaacgaatgt tctggaaagt acgaccaaga
     1321 ccaagcgaag caatttttga tggtgcttta ccgttcaaaa tagtccctgc aagagaaatt
     1381 gtcccctcgc ttggctcata aacacctgtt aagaggttga aaagagtcgt tttaccagca
     1441 ccgtttggac cgataagacc aaccaattcc ccttcatgaa gttccatagt cacgtcacca
     1501 acggcagtca ggccaccgaa gttcttggtt aaatctttta cttcaagtaa tgccattact
     1561 taacctcctt cttcttgagt agagccgcta cgctgaattc cttagttccc aaaagacctc
     1621 caggacggaa aatcatcaag agaataagag ctaatgaata gataatcatt gaaatatcag
     1681 agtaactctt gaggaatacg ttcaaaatac caagtacaat ggctgctaca aaggtacctg
     1741 tgatagagcc caaaccaccc aatacaatga taatcaaaac gttaacagag gtcataaagg
     1801 caaagtcttt cggaacaatt gtcccaacgt atcctgcatg gagaccacct gcaatggctg
     1861 ccgccatggc accaaataca aaggctgaaa ccttaacaaa tgttggattt acacctacag
     1921 attctgccgc aatttcatcc tcacgaacag aaattgtagc acgtcccatc ggactgcgca
     1981 aaaagttaac tgtcaccaag gttgtgatga ttacaaaaat ataaactaaa ggccagcttg
     2041 taaagagtgg aatagacata atacctgctg caccattcgt tacactacca ccattgataa
     2101 ttaaaatacg aataatctct gcaacaccga gggtcgcaat ggcaaggtag tcacctttca
     2161 atcgcaatgt tggaagacct actaagaggg ctacaccacc agcaaccaac atcccaacaa
     2221 tcaaggcgat agcaaatcca ccataagttg gcatcatttt cccaaggata cctactgcat
     2281 aagcaccaat agccataaaa ccagcatgac caagtgaaaa ttgacctgag aaaccaacaa
     2341 tcaagtttaa accaactgcc aagataattt gaataccaat ttgctgaaca atctggatgt
     2401 agtaaaaatt cagaatccct gctgatacaa gaccttgaat aagtccaaaa ccagccaata
     2461 agatagccaa ccaaatcgca ttaactttta aattttgctt catggttaca ccttctcttt
     2521 cacattttta ccaagaatac cacttggacg caccaagagg atgatgatca aaacagtata
     2581 tacgatcgca tcgcggaaag tatcgaattc aatcacatag gtgaatgttt cgatgatacc
     2641 gataacgaag ccaccaagtg ctgcacctgg aataataccg ataccgccca atactgccgc
     2701 aacaaaggct ttcagacctg gggtcatccc catcaaaggc tcaatctggt tgtagtagag
     2761 acctaggaga acacccgcag cacccgcaag agctgaccca agagcaaaag taaagctgat
     2821 tgtacggttt acattgatcc ccataagctg agctgcatcg ctatctacag agaccgcacg
     2881 catggctttc cccatttttg tacgtttgac aattaattga agagcgacca tcaatgcaag
     2941 cgatacacca agaataatca actggatatt ggtaacagaa actggaccaa tgttaaaagt
     3001 cacagcctcg atggcttgtg gaaaggcttt tacgtctgct gtaaagaatt taatcatagt
     3061 gtattctaag aagaaggata caccgatagc cgtaatcaaa gctgctatac gcgttgaatt
     3121 acgcaaagga cgataggcta aaaactctat aacgacaccg agaatagccg tccctaccat
     3181 cgctagaatg agagcaacaa agaaacgcgt tccaccatct gcaataaaag ttaaattatt
     3241 taataggaaa taccccataa aagcacccat catatacaaa tcaccatggg cgaagttaat
     3301 gagtttgata attccgtaca ccatggtata accaagggcc aaaagggcat aaacagaacc
     3361 aagaatcaaa ccattgacaa gttgttgaag cataggttca ccaatctttc taaatatttt
     3421 tcaagctcaa tagcattgtt acattataca aaattttctg aaaataagca agttagatag
     3481 agagtttaat cattaaaatt acagaaaatt taaccaaccc aaatttaacc aaattttata
     3541 tccgcatgta tactattcta tacattctga caagtgaaaa agagggaatt ccctcttttc
     3601 ataaactatt agtcaataga gatagtatct acagatgatt cttctccgtt tgttaaacta
     3661 acaacataaa ctgatttaat gacattgtgc gctttgtcaa cagacattgt accagtcaca
     3721 cctttaaagt ctttcagagc tgcaagatta gccttcactg ctgctgaatc agctgcacct
     3781 tttgctgctt ctgctgccat gtaaaccgag tcgtaagcta aagctgagaa cattgatggc
     3841 tcttcattgt attctttcac gtatgcatca tagaatgctt ttgctttttc gcttgctgat
     3901 gttacaaatc cagaaaggta gtaaacattt gatgctgctg atgcagttgc caattcagta
     3961 aattgtggtg agtcaaaacc atctgaacca agaattggct tgtcaatacc caaaccgcgt
     4021 gcttgcttca caattgtacc tgtttcattg tagtaaccag gcatgatgat cgcatcaaat
     4081 tccttatctt tcaattttgt caaagcagct tggaaatctt tatcaccaga agcaaatgtg
     4141 atttctgaaa cgatttctcc tgtgtatgct ttcttgaacg cctctgctac acccttacca
     4201 taatcagaag agttgtcaaa gtaaagaaca actttcttag ctaataagct ctcagtagcg
     4261 tatttagcca taatttgacc ttgataccca tctgtaaagg ttgtacggaa gaagtattct
     4321 tggacctcac ctttatcgtt cacaaccaag tttgtttgag ttcctgaagg agtaatcata
     4381 ggaacaccag cagatgtcaa tgctggaata gaggctgctg aagctcctga tgtagctggc
     4441 ccaatcacaa cgtttgcccc ttcacttgcc aagcttgttg caacagtagc tgcctctgct
     4501 gtttcagact tgttgtcttt tgtaatcact tcgatttttt tgccgtcaat accaccctca
     4561 gcattgattt ctttcacggc aaggttggca cctttttcct ctgtttgacc ataagatgaa
     4621 acggcacctg acaactccaa gttgtagccg attttaaatg tatcacctac agaagaacct
     4681 gcggctgaag tattggttgt cgaaacatta ccacatgcag cgagtagagc tgcagaggca
     4741 aatgtagcaa gcgctactgc aaattttctt gttttcatga aaactcctta aatctttctt
     4801 aaattttacg atgtatctaa tatactgaat tatctgaaaa ttgtcaacaa aaaaagaaaa
     4861 aatttttaaa aataaaaaat tagcagggaa tacatacctg ctaattccga ctatcctacg
     4921 actgatggtt cttcatctct ccatagatta cccacaaagt tttggtctaa ttccttaatg
     4981 tatgaaggaa ccactttttt tacaaaacgt tcctttttca attttaatat ggtagcttct
     5041 actagttctt gatccatata caccaaaaca tatcgcaagc gtttggaatg gtaaacaata
     5101 tctccatatt ggctgagttt acgtgcatca cgattgtagt gtaaatagac agttaagcaa
     5161 gtgcgatttt tcttctcaaa catgtcttct cctctaaaaa ttctctttct atattatacc
     5221 gttttcaatg ataaatgaaa agctccaact acctgtaaaa gagttggagc taataggaat
     5281 agtcagtact attacatatt tgtcactgct tatttgactt ttgtattgtc cataatctgg
     5341 tcaataaatc cgtaagccaa ggtttcttca gctgacatcc agtaatcacg ttccgcatcc
     5401 ttatggattt gcttgactgt cttacctgag ttgtctgcca agattttttc tagcttatta
     5461 cgtgttttta ataggtgttc tgcagcaata gccatgtctg tttgctgagt accaccacca
     5521 gttccaccca ttggctggtg aatcatgtac tctgcatttg gcaacatgaa acgtttgccc
     5581 ttggcaccgc ttgatgcaat gatggttccc atgctcgcag ctgttcccat aacgatggtt
     5641 tgaacatcag ctttaatgaa attcatcgtg tctacaatgg caagacctgc tgacaccgat
     5701 cctcctggcg tattaacata gaggtaaata tcctttgtag ggtcttgggc atcaaggaaa
     5761 agcaattgtg caatgataga gtttgccatg ttgtcctcaa ctggtcctgt caacatgata
     5821 atgcggtctt tcaaaaggcg tgagtaaata tcataagaac gctcaccacg gctagtttgt
     5881 tcaataacta ctggaatcat aaaattactc cttttcgaga ttttctgtac ccattatata
     5941 tgaatagtca aaattggtca aataaaaaac acgatttcag gaaatgaaaa agaaaccagc
     6001 agagctgatt tcttaattat tttgtaccga acaggcggtc acctgcatct cccaaacctg
     6061 gtacgatgta gcctttttca ttgagttttt catccaaagc tgctgtgtaa atatcaatgt
     6121 ctggatgtgc atcttgaaga gcttttacac cttctggagc tgaaacaaga gcaacaaatt
     6181 tgatatttga agcaccacgt tttttcaatg aatcaactgc aagaatagct gatccacctg
     6241 tagcaagcat tgggtcaact acaaaaatat gacgttgatc aatgtcttct ggtaatttca
     6301 caagatactc aacgggttga agggtttctt catcgcggta cataccgatg tgaccaactt
     6361 tagctgctgg taccaagctt agcaaaccat caaccatacc aacacctgca cgaagaattg
     6421 gtacgatagc taatttttta cctgcaattt gtttctgaac tgttttagtg attggcgttt
     6481 caatctccac atcttcaagt ggcaagtcac gcaaaacttc gtaacccatc agcatcgcga
     6541 tttcattaac caactcacga aaatcctttg ttgaagtaga tgtacgacga aggatagaca
     6601 acttatgctg aatgagtggg tgtgaaatga cttggaattt ccccatgatg ataaaacctc
     6661 tcttttttgt tttttctatt ttaccaaaaa aattgaaaaa atgcttgaga atatggcaaa
     6721 tttatttatt ttttgaaaac gatagcttca aaaggtctca acatcccttt attaatcgcc
     6781 tcttcgtttt cataattgtt catgaaaata gaatattctt ccacagctat agtataagcc
     6841 tgctcctggc ttgaaaagtt tactaatatg atgaaatcat cctgttcatc atatttacgg
     6901 taggccatta ccgagctatt tccagtctcc atcacttcat attttccttc agacatacaa
     6961 ggatactctt tccgaagagc aattaatttt tgataggtat agaaaagcga ttgtttgtct
     7021 gcaagtgctt tttcaacgtt aatgtctttg acagtatctc ccaaagccag ccaggcttga
     7081 cctgtactga aacctgcacc ttcttctgct gtccattgca ttggacggcg agcattatca
     7141 cgacctttgg catttataga agtaatcaat tcctccttgg tgaagccatt ttcaatccgt
     7201 tccgcataca tgcgacgtgt ttcaatatca tccgcttggc taatatccgt aatcggtgca
     7261 ttaatcatac caatttcctc accttggtag atataaggtg tccctgacat aaaatgaaga
     7321 taaatagcta acattttacc acttagttca cgaaatgcag gtcgatcatc accgaagcga
     7381 gaaatggcac gaggtaaatc gtggttattc cagaaaagcg agttccagcc tttaccaacc
     7441 agttcagtct gccatttgga tagaacgtta tgcaaacgtg ctggatcaag cggtgccaaa
     7501 tcccactttt ctttcccttc ctgctgatca agtcctacat gttcaaattg gaaaatcatc
     7561 gaaaattctt ttcgatcagg atgagaatac aactgagaat tttctggatt ggcactccag
     7621 gtttccccaa cagtcactaa gtcgtaagca ccaaaggttt tctcgtttaa ttcctgaata
     7681 agaggatgaa gagtgggacc atcggctgta attaattttt ccggttcttt tccaatcaaa
     7741 tcaatcacat ctagcctgaa accgccgaca cctttttcaa tccaaaaatt gatcatgtcc
     7801 cagatttctt gtttcagagc aggatttttc cagttcagat ctggttgctt aacagagaat
     7861 tggtggaggt agtatttgtt cagatgtgga acgtattccc aagcagaacc tgagaaggtc
     7921 gagcgtaatt cattcggctc atcagcccaa acataataat cataataggg attatctggt
     7981 cctttgaggg cttcttgaaa ccagaaatgt tcatccgagg tatgattgag caccaaatcc
     8041 atgattattt tgatattgcg cttatggcct tctgcaatca gctcttccat atcttccatg
     8101 gtcccaaatt ctggagcaat tccttggtag tcactgatgt cgtaaccatt gtcatccatg
     8161 ggggactgat aaacaggact gagccaaata gcatcaattc caagctcatg gagataatct
     8221 aatcggctga taatccctcg aatatccccg actccatctc catttgaatc ttgaaaacta
     8281 cgaggataaa tttggtaaat gacggacttc ttccaccaat ctgttttttg tatttctggc
     8341 atttgattga tcctttctgt acaatcttat taatgtaaga aaaagaggtg tccccctctt
     8401 tctatataat catattataa ctcaattgtg gtaatttttc cctcttcaat ttcaacaatt
     8461 tcaaaggatt tttcttttag gtttgctgtt aggcgggcag atacccctgc atcttgatta
     8521 ttccagaaaa tttccatact tgtttcatcc aacatcttaa cggagaactc cccgtcgaaa
     8581 gctggacttg tattgcggaa tttaaggagt ttgaacaagg attgtacaac tggccgttgc
     8641 acttcctgct caatttcttc caaatcatag taatggcgat tgatattgcg gccttccttg
     8701 cttgattcca aaagctcaat atcattttcg cctgctagta aaccaacgta gtaaatctgt
     8761 ggaatacctg gtgcaaaaca ttgaagaaca cgcgccaaca gataagcttg atcgttattg
     8821 ccaagggcag aataataagt acagttaatc tgataaatat ccaaattatt ataggcttct
     8881 gtactataaa ttttcttcac attggcacct tgtgcataca aggcttcacg cgtttcctct
     8941 gtctcctcat ccgtcagcaa atccttaacg tccacaacac cgattccatc atgtgtatca
     9001 agagttgtaa attgcttccg agggcaaatc tccatccagt gaaccaggcg attgacatga
     9061 ccagaataca aagcgtggag aactagcatt ggcaaggcaa aatcataaac ataataatct
     9121 tgctcggcaa ttttctgctg aatggtgtag tgttcatgaa tttctggtaa gatttccaca
     9181 tcttttggcg ccaataactg acgtggaaaa tggagcattt cccaaatatc aggctccaca
     9241 aagaaacaat tggttccgat ttttttattg gcataagcaa aggcgtccaa acgaataatc
     9301 gaagcaccat gctctgccaa ctgttccaaa ttttcttgga tgaaacgacg ggttgtctcc
     9361 gttgtcacat ctaaatcaat ctgctgttca tcaaaggtac accacacctt ctcttcacta
     9421 ccatcagcaa atgttgcaat tcgatatgga gcacgaggtt tacgcttata gatcaaatca
     9481 acatcttctt gcgttggctc accatttggc caaaagtttt tatagcgaat aaacaaatcc
     9541 gcataagctg attcatcttt cttttcaaga aaatcaagga aataaggaga ttttgcagaa
     9601 atatgattga tcataaaatc gaacatcaga taatagtcct tgctcaatgc ttcaatatct
     9661 gaccaatctc caaatgcagg atctactttg gtataatcca ttggtgcaaa cccacgatcc
     9721 ccagatgatg ggaaaaacgg cagaatatgg atacctccca caacatcctt taatggtccc
     9781 tcaagaactt ttttcaaatc cttgagattc ttgcctaaac tatcagaata ggtaatcaac
     9841 atagcttggt ttttaatttt catcgatatt ctcctttgta agccttttct ttttcatttt
     9901 ttagaaaagg ttgaacaagt caacctttgt aattatttga cagaaccact tgtcatacct
     9961 gaaataatat gtttctggaa gacaaggtat accaataaga ttgggataat ccccaccaca
    10021 taggatgcaa atgatggtcc ataatcactg aaatacatac cttgatagtt gtattggaaa
    10081 agaggtaaag tccacatgct ttgatcacgg ttcaatacta gtaaaggcaa gaggaaatcg
    10141 ttccagaccc agagggcatt gataatcaaa acggttgcat gcataggttt catgagtggg
    10201 aaaatgattt tccagtacat agtaaattta tcacagccat caatagctgc cgcctcatcc
    10261 atttcttcag gaacaatggt cttaatatag ccgacataga ggaagagggc ctgaggaact
    10321 gcataggtca aatatagaat aatcaaaccg acaatattgt tcaaaccaag gcttgacatt
    10381 aatttggtca ttgggagcat aatcacttgg aaaggtacaa aaatcccaat aatcaagaaa
    10441 aagtagataa agttaaacac tgtctggcgt ttcatttgac gggctacggc gaaagctgcc
    10501 attggaataa caagtagaat caagccaaca gctacaatcg taatcaaggc agaattgcca
    10561 aaatattgta caatcccgtc attaaacaag cgttcgtagt tatttaaggt gaaattttca
    10621 ggaagaccaa agaagttgct cgtgatttcc tttgttggtt tgaacgaact tataatcgtc
    10681 acgagtagcg gaataaacat gaagagaata ccgctgaaga gcacgagata aacccaccaa
    10741 tttttctgtt tttgagtatt catgacagtc ctcctaaatt tcaaatttct tcgaaattct
    10801 catctgaatc aatgagatga caataatgaa gatgaagagt actacagcca aagcattggc
    10861 ataagagaat ttattgtcta caaaggcata gttgtaaacg agtaaaccca aggattctgt
    10921 tttgccatct gggccaccac ttgtcaatgc aaagataaga tcaaatgctg tcaaacctga
    10981 tttcatagcc aagatgaata ccatactgat agatggtaat aagaatggaa gctcaatgtt
    11041 gaagaactgt tgagaacgac ttgcaccatc aatagctgct gcttccttca catcttctgg
    11101 aatactttgt aagccagaca ggaagatgat gactggcata gccaatcctt gccagagagc
    11161 cacaaaaagt acagctggaa taacagtatt ttcttgtacc aataagtttt ccatcaacca
    11221 atcgatatga aggatttctc caatctgtgt aaaaccatag ttgaacaatt ggacaaaaat
    11281 caagcctaaa gtaacggttg aaagaaccgc agggaagaaa taccaggttc taaagaaacc
    11341 aacagcttta atcttacgat tgaggagact agccaaccaa atccctataa caatttctcc
    11401 gatgaccaaa ccaatggtga agataatggt aaaagtcatg gaagtgtaaa aatcaggatt
    11461 ttttaaaata tctaagtaat tctgaatacc gattagtttg aaatcagatg tcaatccatt
    11521 ccaatccgtc aaactataat agatacctgt aaatagggga tagaagaaaa agattaactg
    11581 taagccaatt ggtatggcaa caaatagata gggccaatat ttggcaataa atcctttttg
    11641 attcatcatg gtactccttt ttgataaagt tatagttgct tagtctcagt tcaaggaatc
    11701 ccatagggtt actaaaactg acactaggcg actataagga ggctaggcta caactagccc
    11761 ctgcttatag ttttcctgga ttattttttc attggatcaa agaatgcgtt cagttttttc
    11821 actaattctg ctgagtttgg atttttaacc atttcaacag tgatattcca gaactcttct
    11881 tctgattccc attcagattg caaccaaact acgtgtttgt cagtgaaggc atattgtgta
    11941 actccagcag tttcttcaaa cttaccttct gtatctacac cttcaactga agttggtgag
    12001 ccatctacat catagtattt ctggataact tctggacgag agaggtattc taggaatttt
    12061 ttagactctt ctgggtgctc tgtatctgct gaaatagata gagcaaggtc agctgcaccg
    12121 atggtatagt cgccaccttc tttatcgcca gggaatgtaa acataccata ttcaaattct
    12181 ggttcttgtt ggtttacagc tgtagctgcc catgtacctt gtggaagcat gagagcatca
    12241 cctgctgcaa aggctgccac tgcatctgca tagagcgcac ctgttgcacc tttttgacca
    12301 ttatctgtca acagttgcaa gcgttctaaa actgcttttg ttgtcgcatc atcttgaatc
    12361 gctccctttg cactacgaat cagaatatct tctgcaccat caaatccacc cgcaacagtt
    12421 acccaagcta actgatggta gccgttcaat gaccaagcat cgttcaatga aagagcaaac
    12481 ggtgctgcac tgccatcaga cttgattttg tctaccaaag ccacaaattc tgcataggta
    12541 gttggaactt cgagccccaa ttctttgaac ttatccttat tatagtaaat accataggca
    12601 ttagctgtca aaggaagagt gtaatttttt tcgttaacaa gataaggtgt tacagcacca
    12661 tcttttagat gactgaggcc tgcatcatca ccaatttcta ggaaacgacc atccgctgcg
    12721 tatgctttga agtccgcatt ttgtggataa atattgatca catccggtgc ttcattgtta
    12781 gccatacggg ttttcaaaac agttcctgca tctggtacat ttgaaaattt aacatcaatc
    12841 gtaggatttt ccttttcgaa atcattaata atttcctgaa gagtagcctg catttcaggt
    12901 ttttgtgaga aatattcaat ttctacctta tcgctactac ttgcattccc acaagcaacc
    12961 aagaagctag caaaagcaca aaccgatgca cattttaaaa acgtttgcat tttcattata
    13021 aaatcctcct agattttgtt aaaaatatat tgtacactaa aataatcttt ttgaacatgt
    13081 ggcattgtaa gaccgatgtt catcagttca tccccgtaga atgtttctcc caattgaggg
    13141 cattcataca aggcatccgg gtctagcccc ttcaaccgga catgaagcaa tggtgcctcc
    13201 ggtttagcaa gaatttttac aaatgtaaag accgcttgat tcttatcttg actaacataa
    13261 ttggcagccc aggtattggt cgtctttttc agacggtaga gctgaccgaa ttgtatagtt
    13321 tcacgaatgg agtgataggt ttcgatttgc tgactgattt catccaattc cttttccgaa
    13381 agtttcgtta aatccagttc gtaaccaaat gctcctccca tcattgccac attgcctcgt
    13441 gtagcaagag gtgtgatccg accaacctga tggttcggaa cggcagaaac gtgtgcacca
    13501 attgaggaag tgggataaat aaaactagta ccttcttgaa tagataagcg ctcaatcgca
    13561 tctgtatcat cacttgccca agcttgcggc atgtaataca tgattcccaa atcattacga
    13621 cctccaccgc ctgcacagga ttcgaaaaga atatccggga accgctttgt aaggtggtct
    13681 aatacacggt agagacctag catgtaacga tggtggaact caaagcgctg gtcatttgct
    13741 aaaccttcag gaatattggt gatattacgg ttcatatccc attttacata agaaatattg
    13801 gctgattcta atatggaaga gacgctatca atgatgtagt cacagacttc ctgcttggtc
    13861 aaatccaaaa ctaattgttc tcggctgtag attggtaagc gtccttctgt gcgaattgcc
    13921 caatcaggat gctcacgata aagatcgcta tcaatagaaa tcatctctgg ttcaaaccac
    13981 aacccgaact tcataccaag ctcatttatt cgctctgcta aaccattcaa accatttggc
    14041 aacttatcca aattgacttt ccaatcgccc aatgaacttt catcattgtt tcgtttacca
    14101 aaccaaccat catccagaac aaacaattca ataccagctc gacttgcaac ctgagctaac
    14161 tgtaaaatct tttcttctgt aaattcaaaa taggtcgctt cccagttgtt gattaaaatg
    14221 ggtcttggtt tgtttacaaa cgatgatcga atgaggtgtt ttgtgataaa gtgatgacta
    14281 ttttgtgtca tgccagtaaa tcctttatca gtgtatgata agagaacttc tggagtttga
    14341 aaccggtcat ccttatccaa ttgccatgag aaatagtgac tttccaatcc tagtcccaat
    14401 cgactggttt ccatggctgt cgtttcgaca aatgctgtaa agttgccgct atagacaagg
    14461 tgggccgcat aaacatcgcc cttgtcctca cttgcatcgg gtgaggctag tgctaagaag
    14521 ggtgttcttg agtgacttga agcacctcga atgctaccaa tcgaatactg accttttgtc
    14581 aatggggtct gtgtccactc tttttcataa gcataacgtc ctgccaagct gtgaacaatg
    14641 aaatcttgat gtggaaaatc aagcgttgca gacaagacct tctctagttt gcatggatat
    14701 ttgccagttt gaattgtagc tgaccgtgcg agataggaag cttcttcaaa aacagaatac
    14761 tgaagagtta cggtaatatc tgtcaactga tcgtaaagat caatttcaag actctctacc
    14821 tcttcttgat ttccaaaact tgctggtaat cccctcagtt cttttttccc cttgtataat
    14881 cgatactctt tgtattttag gtcaagagtg actccaaatt cattccgaac atctaatgct
    14941 gaggttctaa aatctcccag accattgctg gaatattcga gtgggaggac atccaatgag
    15001 tatgtcctat ccccagtaat tggactcgga gaaaacgatc gatctaaata tgttattttg
    15061 tttccatcgc taaatttttc gatttttttc ccaaaataac gatgaaccag atcgcctgtt
    15121 tccaacactt ggatgatata agagaattct cttgttttca ggtgaaaaat ctgtttttca
    15181 ttataaatct caatcacatt catctctccc atctcttctt tatggtctta ttgtacaaaa
    15241 tgaaaacgtt ttatttaata gtcttttgta tttaaaacat gttataatgt ttcctaggag
    15301 gattgaaatg aatattctaa atatctataa tgaattggat agtcataatt ttgacctaaa
    15361 tgtagaccat tacggtgccg aacaatgtga caagggctat tccttcggtc ctactatccg
    15421 tgataatttt gtcctgcatt ttattacaaa aggaagagga aaaattcttg ttgacggaaa
    15481 aacaagctac cttgctgcag gcgatttgtt tattcttcca aaggatgttt ccatttttta
    15541 ccaagctgat gaaaatgaac cttggactta tatttgggtt ggttttagtg gttcacgagc
    15601 aaaagatcta cttaatcaaa gtcaactgct agaaaactac tacctccact ccaatctaga
    15661 gtcaccaatt ctaggctaca tgcatcagat taaccatgtt cagatgcatc ccatcccctc
    15721 tatcacagaa ttggtcttaa ttggctacct caaccaactc ttagcagcac tcatcgagga
    15781 atttcccagt gaaacattaa ttaagcctga aacacaaacc aagtactatg ttcagcaagc
    15841 catcaagatg atccataacc actatgccca tccaattaaa gtttctgaaa tagcagattt
    15901 tctagcttta agccgaagct atctctataa aatttttaag caggaaactg gctattctat
    15961 caaagattat attttacaag taaaaatgaa tcgttcttgt caactgttag aagatgcttc
    16021 tcggagtatc actgaaatcg cctattctgt cggataccaa gaccctctca ccttcagcgc
    16081 ggccttcaaa aattatttcc atatgagccc gacagaattt agaaaaacac aaaaaaataa
    16141 ggattaggtg attttactca cacctagtcc ttatttcttt atccaaaaac cttaccaagt
    16201 cgctgacagg cttcttcaat catgttgaat ggtgctgcta cattcagacg tgcatggtgc
    16261 ttaccttcct taccaaaggt caaaccatca ttcaaaatca acttcgcttt acaatgaaga
    16321 atatcaaaga gctccgcatg ctctaactca taagcagaaa aatcaagcca gatgaggtag
    16381 gttccctggg gcttcatgac cttgatcctt gtatgggttg tcaaatactc ttcaacaaag
    16441 tctatatttc tctccaaaat aaccttcaac tccttcaacc actcatctcc ataagtgaaa
    16501 gcagcttctg tggttaacaa gccaatcgtt ggaatctcgt gctgattatt tatcaattga
    16561 cgcttagcga aagtcttgcg aatgcttgga ttttcaatga ttgcaaagct attttttgta
    16621 ccagcgatat tgaaggtctt ggtcgcggat gataagatga ttgaaaactc tttaaaactt
    16681 tcatccactg tgttaaagga ataatgcttg tgaccataca aggtcaaatc ttgatgtatt
    16741 tcatctgaaa caaggataac accatgtttc tgacaaagac gtccaatggc caacacttcc
    16801 tctcttgtcc aaactctacc acctgggttg tggggattac agaaaatata gagtttcact
    16861 tgattctcta caatgtcctt ttcaagctgg tcgagattaa gcctaaaaat accatccaca
    16921 tctacaagtg aattggtgac tagctgacga ttgtttaatt ttacagtccg cgcaaatggt
    16981 ggatagacag gtgtattaat taggactgca tccccttctt ctgtcaaagc ttgaatagcc
    17041 acagataagg caggaacaac tccttcaatg agtaggacgg actctctttc aatcttatag
    17101 ccatgctgtc tatcttccca gtcaataata gactgataga ggctgtcact agcataagga
    17161 taaccaaaaa catgatgatc tgcgtagtca cggattactt ggcgaatttc tggtagaggc
    17221 tcaaagtcca tatctgctat ccacaagggt aagagttctt tatccgtctc tacttttctc
    17281 catttttccg catgctggga taaacgattc ggactatcta taaaattata ctgtgtcata
    17341 tcttatccct ccaaggctgt ttttaaatct acaatcaagt cttccgcatc ctctatacca
    17401 atagacagac gaagcaaatc atctgtcaaa ccgtaagaat gacgggtttc agctggaatg
    17461 tctgcgtggg tctgagtggc tggataggta atcagactct ctactccacc cagactttca
    17521 gcaaaggtaa agactttcag ggtgttgaga atatggggaa tccgactttc atctaccacc
    17581 ttaaccgaaa tcatcccacc ttttcctgtg tagtaaactt gcttgactgc tggactcttt
    17641 tccaaatagg caacaatttt ctgagcattt tgtgtcgccc gctccattcg aagcgagagg
    17701 gttttgagtc cacgcatgag taagtaagaa tccagtggag atagggttgg acccgatgta
    17761 ttttggtcat aaaagagctt atcatatagg tcttgatcat tggtcatcaa agcgccagct
    17821 agtacatcat tatgcccaga taggtacttg gtcgctgaat gtaggacaac atccgctcct
    17881 agaacaagtg gattttgata aatagggctg taaaaagtgt tatcgacaat caccttagca
    17941 cctttggcat gggcgatagc cgaaacctta gcgatgtcaa actccaccat gagtgggtta
    18001 gtcggtgtct ccagataaac atagtctgtc tcttcggtga tggctgcaat cagctcctcc
    18061 tcggtgtttg cataagtaaa ttgaaatcta ccgatactct cttgctcatt gaaccaacgg
    18121 aaggaaccac cgtacaaatc acgcgccgct accacttgac tccctacagg aaaaccatta
    18181 aaaagtaaga ccagagcagc cattcccgag ctcgttgcta gggcatagtc ggccttttca
    18241 atagctgcca aagtcgtctc tagactggca cgtgtcggat tttttgttct cgtgtagtca
    18301 taacctgtcg attgaccaaa ctcagggtgt tgataggtcg tcgaaaggtg gataggggaa
    18361 atcaaggcac ctgttctctc atcgctattg atgcccgtat gcgctaaaat cgtatcaatc
    18421 ttataatccg tcataacatc tactccaatc taacttacaa tatagtctag catgaaatag
    18481 gagatttggt gtgaaaaata tccaaaaata cttatagaaa aatagcaagc tttgttatag
    18541 tttatcatta taacaacatc tcccccttac atatacttgc tggattatcc attattaatt
    18601 tgatataatg acaaagaggt ggtcattata aaaatcagac tcaacaaaca cttctaccgt
    18661 ttcagtaatt tttattctat ggtgttttgg atttgttttc tgctaccaac tgccaatgat
    18721 agctttgatc agtggccact ctggtataca agcctaattc taataatcat tcctattcat
    18781 attttcaggg cttttttcct aaaagaagaa aaggagctca ctcgcatagg ttatatcttc
    18841 tatatcggat tgattgtggt gattatctat ctgaaattca ttttgggtaa atagaaagga
    18901 gcttactatg tctaaaaaat tcaataaagc attctatcgg acctgggaat tttacttgtc
    18961 tcttgcctgt atcctacaag tgatttctac tgctatacat ggaccatttt ggtttatcca
    19021 ttttctcaag cttggagtcg gactttatgc tggttatcta gctttcttta agtcacgtag
    19081 ctggattaaa aaaacatggc aaatttatct ggtgattgga ttgattgcga gttggacaat
    19141 cttttacttc tttgtactgt gaggattttc aatgtttaag gaatttaata gacttgttcg
    19201 gtaactacat catctcatgg ttgatgatgc cacaaatcaa ctcagctctt
//
LOCUS       DATUCG010000032        19045 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.033, whole genome shotgun sequence.
ACCESSION   DATUCG010000032 DATUCG010000000
VERSION     DATUCG010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19045
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.033"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            <1..367
                     /locus_tag="VCA20_000889"
     CDS             <1..367
                     /locus_tag="VCA20_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HEP1834736.1"
                     /translation="QYLNKNNIEILTGDFEKAVEDVGAGDFIYFDPPYIPLSETSAFT
                     SYTHEGFSYEEQVRLRDVFRKLDKKGAYVMLSNSSSLLVEELYKGFNIHKVEATRTNG
                     AKVSSRGKISEFIVTNYDK"
     gene            357..1193
                     /locus_tag="VCA20_000890"
     CDS             357..1193
                     /locus_tag="VCA20_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEP1834737.1"
                     /translation="MTNNEYKYGGVIMTKPYYHKENSILVHADTFEFLSKMKPESMDM
                     IFADPPYFLSNGGISNSGGQVVSVDKGEWDKVSSLEEKHNFNRRWIQLAKKVLKPNGT
                     IWISGSFHNIYSVGMALEQEGFKILNNITWQKTNPAPNLSCRYFTHSTETILWARKNY
                     KKARHYYNYDLMKELNDGKQMKDVWTGGLTAKSEKWAGKHPTQKPEYLLERIILASTK
                     ENDYILDPFVGSGTTGVVAKRLGRRFIGIDAERDYLKIAKTRLEKESGKKIETLKNGC
                     LP"
     gene            1190..2017
                     /locus_tag="VCA20_000891"
     CDS             1190..2017
                     /locus_tag="VCA20_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HEP1834738.1"
                     /translation="MTDTIADWTYYTDFPKVYDNVESIKVPLSIMNSLIGSKNIQQDF
                     LDLYQNYPEVLKVIPILIAKRLKDTIIVKDPIKDFYFDFRKQNYSIEEYAMFLEKSGI
                     FDLLEKHIISNLVDYVTGVEVGMDTNGRKNRTGHAMENIVQNYLEAAGYVLGESLFKE
                     IYQNEIEELFSVDLSAITNEGNTAKRFDFVIKHNSTIYLIEVNFYSSGGSKLNETARS
                     YKMIAEEAKIIPNVEFMWFTDGQGWHKAKKNLQETFEVLPHLYNTNDLKNNILKTLK"
     gene            2291..3352
                     /locus_tag="VCA20_000892"
     CDS             2291..3352
                     /locus_tag="VCA20_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEP1834739.1"
                     /translation="MQARVEKFEAKLAQSDVDGILVTGQNNIYYLTGFWGTEATVFIS
                     GKRRLFVTDSRYTLIAKASVKGFDIIESRFALEEIAKVIKEDGLETIGFDSQVTYGFY
                     QSLTNIFEGYQLDAMSNFIEDLRMIKDEKEIATIRRACQISDQAFIDVLDFIKPGQTT
                     EMDVNHFLDHRMRQLGAEGASFEFIVASGYRSAMPHGRASEKVIQAGETLTLDFGCYY
                     QHYVSDMTRTIHIGHVTDQEREIYDVVLRANKALIEQAKEGVTYREFDAIPREIINEA
                     GYGANFTHGIGHGIGLDIHEYPYFGKSDEPIKAGMVLTDEPGIYLDDKYGVRIEDDLL
                     ITETGCEVLTLAPKELIVI"
     gene            3417..3974
                     /gene="efp"
                     /locus_tag="VCA20_000893"
     CDS             3417..3974
                     /gene="efp"
                     /locus_tag="VCA20_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEP1834740.1"
                     /translation="MIEASKLRAGMTFETEGKLIKVLEASHHKPGKGNTIMRMKLRDV
                     RTGSTFDTTYRPDEKFEQAIIETVPAQYLYQMDDTAFFMNTETYDQYEIPVATIKEEL
                     LYILENSDVKIQFYGTEVIGVTVPTTVELVVAETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIEAGQKLVINTAEGTYVSRA"
     gene            4001..4393
                     /locus_tag="VCA20_000894"
     CDS             4001..4393
                     /locus_tag="VCA20_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEP1834741.1"
                     /translation="MTTEYQGEIVIAPRVLEVITGIAAAKVEGVHSLHNKRVADTWSK
                     SSLNKGVYLQTDDEGKVTADIYVYLEYGVNVPAVSMDIQRAVKTAIYNYAEVEVTAVN
                     IHVNGIVPDKTPKPALKDLFGEDFLDEE"
     gene            4383..4802
                     /gene="nusB"
                     /locus_tag="VCA20_000895"
     CDS             4383..4802
                     /gene="nusB"
                     /locus_tag="VCA20_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEP1834742.1"
                     /translation="MKNNRHALRKCALQAIVSLEFGQEPVQAAQFSYLYDREEEQEGV
                     EIPLFLLNLVNGVADYRDDLDKELSSRLKAGWTLDRLTLIDKNIMRLGLFEILHFEET
                     PDRVAVNEAIELAKEFSDESSAKFVNGVLSQFIKEEA"
     gene            complement(4882..5847)
                     /locus_tag="VCA20_000896"
     CDS             complement(4882..5847)
                     /locus_tag="VCA20_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEP1834743.1"
                     /translation="MVAKLTDVAQLAGVSPTTVSRVINKKGYLSEKTIRNVEEAMREL
                     GYKPNNLARSLQGKSAKLVGLIFPTINNIFYSELISHLEKELFDRGYKTIICNSQHES
                     DKEREYLEMLAANQVDGIISGSHNLGIQDYDRVVAPIIAFDRNLSPSIPIVSSDNFAG
                     GVLAAQTLQKAGCHNPLMITGNDDSNSPTGLRQVGFTSILNKAEVFHISSDFSPVRKE
                     MEIRAILEKQKPDGIFVSGDATAMLVWNVIRSMKLSIPEDIKLIGYDGTSFIENYYPQ
                     LTTIKQPLTEIARLLVELLVDKIEGKKLTKTDYILPISLLAGASI"
     gene            complement(5828..7282)
                     /locus_tag="VCA20_000897"
     CDS             complement(5828..7282)
                     /locus_tag="VCA20_000897"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HEP1834744.1"
                     /translation="MAFTTEERYRAYADWTLEYIEKIKKNTESSPWRTNFHIETPHGL
                     LNDPNGFSYFNGKWTLFYQYFPFGAAHGLKSWVHTESRDLVHFEETGTVMYPDTPLDS
                     HGAYSGSAMEFGDKLFLFYTGNVRDENWVRHPYQIGALMDKDGKIEKIDKVLIEQPED
                     TTDHFRDPQIFNYKGQYYAIVGGQNLDKKGIVKLYKAENNDYLKWSYVGDLDFANDMT
                     AYMMECPNIAFVGEQPILLHCPQGLDKEVLDYGNIYPNMYKIGQSFDPETASIVEPSE
                     LINLDYGFECYATQAFNAPDGRTLSVSWLALPDVEYPTDAYDYQGCLSLVKELTIKDG
                     KLYQYPVNAITSLRKEAQPFSAQKETNNVYELELDFSAGTKHEIVLFANEEEKGLVLS
                     VDTEQGQITLDRGNCGQPFALDFGTSRSCEISPGALTANIFIDKSVFEIFINKGEKVF
                     SGRVFPDADQSGIVITTGNPTGTYYHLDYGRKTN"
     gene            7488..9434
                     /locus_tag="VCA20_000898"
     CDS             7488..9434
                     /locus_tag="VCA20_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HEP1834745.1"
                     /translation="MDYKKVSAEVIEAVGKDNLVAAAHCATRLRLVLKDESKVNQKAL
                     DDNADVKGTFSTNGQYQVIIGPGDVNFVYAELIKATGLKEVSTDDLKQIAAQDKKFNP
                     LMDLIKLLSDIFVPIIPALVAGGLLMALRNFLTSPDLVGQKSIEEMYPAIQGISAMIQ
                     LMSAAPFMFLPVLVGISAAKRFGANQFLGAAIGMIMTTPDLGGKEAFWDIFGFQVAQT
                     NYAYQVIPVLAAVWLLATLEKFFHKKLPSAVDFTFTPLLSVMITGFLTFTVIGPVMLT
                     VSDAITNAIVWLYDTTGALGMSIFGGTYSLIVMTGLHQSFPAIETQLLSAYNNNGTGY
                     GDYIFVVASMANVAQGAATLAIYFLTKNAKTKGLSGSAAVSAFLGITEPALFGVNLKY
                     RFPFFCALAGSAIGALFAGLTHVIAVSLGSAGFIGFLSIKASSIPMYVVAEVIAFAAT
                     FAFTYVYGKTKASAVFADEAATAAATAVTASVVETSEEVEETAEIFTAQETLVSPLSG
                     DVVALENVNDPVFSSGAMGKGLAVKPSEGVVYAPADAEVTIAFETGHAYGLKTAYGAE
                     LLIHIGIDTVSMNGNGFEKLVAAGDKVKAGTPIAKFDAAKIAEAGLDDTTMVIVTNTA
                     DFAEVSPLAEGAIAHGANFLKVAK"
     gene            9563..10447
                     /locus_tag="VCA20_000899"
     CDS             9563..10447
                     /locus_tag="VCA20_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEP1834746.1"
                     /translation="MTKLYGSLEAGGTKFVCAVGDENFQVVEKTQFPTTTPYETIERT
                     VEFFKRYEDRLAGIAIGSFGPIDIDQNSQTYGYITSTPKPHWSNIDLLGLIAKEFNIP
                     FYFTTDVNSSAFGETLVRKGVKSLVYYTIGTGIGAGAIQNGEFIGGLGHTEAGHTYVA
                     LHPYDVKHEFKGMCPFHNGCLEGLAAGPSLEGRTGIRGELIELNSDVWDVQAYYIAQA
                     AVQATLLYRPQVIVFGGGVMAQEHMLNRVREKFVGLMNDYLPTPDVKEYIVTPAVAEN
                     GSATLGNFALAKQVSRNS"
     gene            11061..14516
                     /locus_tag="VCA20_000900"
     CDS             11061..14516
                     /locus_tag="VCA20_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase"
                     /protein_id="HEP1834747.1"
                     /translation="MIPIQQHGKRWLILTLTLSTSVLALASIGASQPVVFADENSHLQ
                     SQENTNKIEVLDWEAFSKKLKDYSSDQRQFHVLKLGFENRLGTLSTREELEEFGKNNN
                     FLVINGKVTQNIHDFPHILVMNKGDVIAHNEEEYASLTKDLRFAGDYKSTVSDIGYHP
                     QRVHALFQIQLDSHKRRLLEAAGLGTADNSMRNVNGFNIYSHGLSIDNKYYANYGNFN
                     KYDNLNVTRERFDKNDQTIQKLIRDSGAFSEQLDREKIKTFAVYVANRVTYDWTAANK
                     AVTNQADVNYYLGSDLFAVTEQQKAMCVGFSTTAARAFNMMGIPAYVVTGNNRSGVPH
                     ATTRAYYDGKWHTIDATGFINGTVRNSAIYSSHHFDTIGEDSYEVVDKGKLEELPLNS
                     KNYMVIDKEYEEWAKKQKAADLLLVNSDKAFVAKEYIAYIEPTYVSQENKKHLLDIYK
                     VLREKIEEAKAKDLNSEHKNGYESILKTAVSDIDKLNNSVNITEKEFDEIKNSMKNNK
                     GFLSQLDKDAAASFEDGNDYKTYLAIMDGKKLQEDPTIIKDNHNVSENKLSPLKEENQ
                     ANSDEVSQNSKDASAPSVNSAQQSEELEGTTSTQETISAAPSQQTPAPKALQAKTELE
                     DKTETSSSSFEKESNEVSANIVLSPEITKNTGSLASVESRVEEAVDGTTPSEKVSAES
                     EEVEKTIVSEVSSDASLANENKNSDAQEDTPRLEKSAEVVTTSQEVAEAPVSAPQVSS
                     EIPTSSETAPTEVATEAPELSALESNPAPQTSLETTPTEEVTETPEPSVVESNSASPT
                     SPETNSAEAVTTSQEMAGTSVSAPQVISEIPTSSETAPAEVATEVTEPSVVESDSASP
                     TSPEVNSTEAVTTSQEMAGTSVSAPQVISEIQTSPETAPAEVATEVTEPSVAESNSAS
                     PTSPEVNSTEAVTTSQEMAGTSVSVPQVISEIPTSSETAPAEVATEVSEESSSASQPS
                     SETNSTEAVTTSQEKADSLVSAPQTSSASLTLEVPKAEHLDEKADATMPNGVEANTHE
                     AGAVPTSDIYVQDAGSDTVPQPQSLSATVLREAISTVEPVSVATSSQERSAVAGEEKV
                     PASLEPSNNSVKEEKVVDSNTTIENQETEKKEVLTSENVLNSLVTIWVALSTSFFMRY
                     FSRGK"
     gene            14836..17361
                     /gene="secA"
                     /locus_tag="VCA20_000901"
     CDS             14836..17361
                     /gene="secA"
                     /locus_tag="VCA20_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEP1834748.1"
                     /translation="MVTNVLRSLIENDKGELRKLEKMADKVFSYAEEMEALTDEQLQA
                     KTAEFKERYNNGESLDDLLYEAYAVVREGARRVLGLYPYKVQVMGGIVLHNGDVPEMR
                     TGEGKTLTATMPVYLNALSGQGVHVVTVNEYLSTRDATEMGELYSWLGLSVGINLAAK
                     SPLEKREAYNCDITYSTNSEIGFDYLRDNMVVRAEDMVQRPLNYALVDEVDSILIDEA
                     RTPLIVSGAQGSETNQLYFLADNLVKSLTTEDYIIDIPSKTIGLSDSGIDKAEKFFKL
                     DNLYDIENVAITHFLDNALRANYIMTYDIDYLVNEDQEVMIIDPFTGRTMEGRRYSDG
                     LHQAIEAKEGVPVQNESKTSASITYQNLFRMYKKLSGMTGTGKTEEEEFREIYNIRVV
                     PIPTNRPIARVDHEDLLYPSLEYKFNAVIADVKRRYEKGQPVLVGTVAVETSDLISQK
                     LVAAGVPHEVLNAKNHYREAQIIMNAGQRGAVTIATNMAGRGTDIKLGPGVRELGGLC
                     VIGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMKRFGSERIKVFMERMNLT
                     EEESVIKSKMLTRQVESAQKRVEGNNYDSRKQVLQYDDVMREQREIIYRQRQDVITAD
                     RDLAPEIKAMMKRTIERQVAGHFLGSKDEAIDGIIKFAHANLVEDDTLSKATFEAMNQ
                     KEIVEELYERALRVYDSQVKKLRDEERVREFQKVLILRVVDNKWTDHIDALDQLRNAV
                     SLRGYAQNNPIVEYQSEAFTMFNDMIGAIEFEVTRLMMKAQIHDNIERERTSQEAHTT
                     AVKNIMPNQSHAIQENVSFEGVDRNDPCPCQSGKKFKNCHGRK"
     gene            17476..17835
                     /locus_tag="VCA20_000902"
     CDS             17476..17835
                     /locus_tag="VCA20_000902"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEP1834749.1"
                     /translation="MIVGHGIDLQEMEAIESARIKHQGFPKKILTEKEFERYQSLSGR
                     RQLEYLAGRWSAKEALTKALGTGIGKIGFHDIEILNTSKGVPYVTKSPFDGNIWLSIS
                     HSGNFVQASVILEEKDD"
     gene            17828..18931
                     /locus_tag="VCA20_000903"
     CDS             17828..18931
                     /locus_tag="VCA20_000903"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011226530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEP1834750.1"
                     /translation="MIESEHRPTQIRVNLDTIAENFEQVMTNLSPKTEAFAVVKANAY
                     GHGAVAVAKKLSSQAAGFCVSNLDEALELRKAGIEHPILILGVVPVRFLPVAHQLNIS
                     VTVASLDWLQDAKDLEADLSGLTVHLKLDTGMGRIGFRKIADLLQAIAILEELEYDIE
                     GVYTHFATADEVNQAHFESQLSVFQEFLDVLPITPRWIHASNSATSIWHADTVFNLVR
                     LGNILYGLNPSGRVLELPFDVQPALSLVSEIVHVKQVEAGTTIGYGATYHSVDSEWIA
                     TVPIGYADGLVRSLQGLSVLVNGQACEIVGRISMDQITIRLPHAYPLGQKVTLIGQDE
                     DQTISVQEWADRIGTINYEVVCLLTDRLPRIFE"
BASE COUNT         5954 a         3416 c         4120 g         5555 t
ORIGIN      
        1 acaatatcta aataaaaata atattgagat tttgacaggg gatttcgaaa aagctgttga
       61 agatgtaggt gctggagact ttatttattt tgacccaccg tatatacctc tttcagaaac
      121 aagtgccttt acatcttata ctcatgaagg tttttcctat gaagaacaag ttagattgcg
      181 agatgttttt agaaaactag ataaaaaagg tgcctatgta atgctatcca attcgtctag
      241 tctgttagtg gaagaattat ataaaggttt taatattcat aaggtagaag ccactcgaac
      301 taatggggcg aaagtatcaa gtcgaggaaa aatatcagaa tttatcgtaa ctaattatga
      361 caaataacga atataagtat ggaggggtta ttatgacgaa accatattat cataaagaaa
      421 attccatact tgttcatgcg gacacttttg agtttttgtc aaaaatgaaa cctgaaagta
      481 tggatatgat ttttgcggat ccgccgtact ttttgagtaa tggcggaata tctaattcag
      541 gtggtcaagt tgtatcagtt gataaaggtg agtgggataa agtttcctca cttgaagaaa
      601 agcacaactt taatcgacgc tggattcagt tagctaaaaa agttttgaaa cccaatggga
      661 cgatttggat ctcaggtagt ttccacaata tttattcagt tggaatggct ttagaacaag
      721 aaggtttcaa aattctgaat aatattacat ggcagaaaac gaatcctgca cccaatttat
      781 cctgtcgtta ctttacgcat tcaaccgaaa caattctttg ggcaagaaaa aactataaga
      841 aggctcgtca ctattataac tatgatttga tgaaagagtt aaacgatgga aaacagatga
      901 aagatgtctg gactggtgga ttaacagcaa aatcagagaa atgggctgga aagcatccaa
      961 cccaaaaacc agaatatctt ttagagcgaa taatattagc tagcacaaaa gaaaatgact
     1021 acattctaga tccttttgtt ggtagcggaa cgacaggagt tgtagcgaag cgattaggta
     1081 gaagattcat tggaatagat gccgaacgag attacttaaa aatagcgaaa acgagattgg
     1141 agaaagaaag tggaaaaaaa atagagactt tgaaaaatgg ttgtctacca tgacagacac
     1201 tatagcggat tggacttatt atacagattt tccaaaagtc tacgataatg tggaaagtat
     1261 aaaagttcct cttagtatta tgaatagtct tattggtagc aaaaatatac aacaagattt
     1321 tttagatttg taccaaaatt atccagaagt tttgaaagtt ataccaatcc ttattgcgaa
     1381 acgtttgaaa gatacaatta ttgttaaaga ccctataaaa gatttttatt ttgactttcg
     1441 taagcaaaac tatagtattg aagaatatgc tatgtttttg gaaaagtcag ggatttttga
     1501 tttactcgag aagcatatta tatcaaatct agtggactac gttacaggag tcgaagtcgg
     1561 catggatact aacggtagga agaaccgtac tggacatgct atggaaaata ttgtgcaaaa
     1621 ttatctagag gcggctggtt atgttttagg tgaaagccta ttcaaggaaa tctatcaaaa
     1681 tgagattgag gaactcttct ccgttgattt atccgccatc actaatgagg ggaatacagc
     1741 caaacgattt gactttgtta tcaagcataa ttcaactatt tatttgatcg aggttaattt
     1801 ttactcaagc ggtggttcaa aattaaatga aacagcacgt tcatataaga tgattgcaga
     1861 agaagcgaag ataattccta atgtagaatt tatgtggttt actgacggac aaggctggca
     1921 taaggctaag aaaaatttac aagaaacttt tgaggttttg cctcacttgt ataataccaa
     1981 cgatttaaaa aataatattt taaagacttt aaaatagttt aggcaatgat taaattttcc
     2041 ctaaaaatta gccgaaggcc ctagtgtagg cgtatccgat aaaatgcgta tgttctttat
     2101 gggaaatgct ctcgtaactg aaatcgtacg aaaaattgga gagtatataa aagtattgga
     2161 ttagtgaaag attccatttc tctgtgacat acttaaaaca gtcgctctag cggctttttt
     2221 gtctgctgaa aataattggt aaatcctagt atttctgcta taatagttac aaaatctttg
     2281 gaggtagctt atgcaagcgc gtgtggaaaa atttgaggca aaattagccc aatctgatgt
     2341 ggatggaatc ttggtgactg gtcaaaacaa tatttactac ctgacaggtt tttgggggac
     2401 agaggcgact gtcttcatca gtggcaagcg tcgcttgttt gtgactgatt cgcgttatac
     2461 cttgatagcc aaggcatcag taaaaggttt tgatattatc gagagccgtt ttgcccttga
     2521 agagattgca aaggtgatta aggaagatgg gcttgaaacc attggttttg atagccaggt
     2581 gacctatggt ttctatcaaa gtctgaccaa catttttgag ggttatcaac tggatgctat
     2641 gtcaaacttt atcgaagact tgcgcatgat taaggatgag aaagaaattg cgaccattcg
     2701 ccgtgcatgt caaatttcgg atcaggcttt tattgatgtt cttgacttta tcaaaccagg
     2761 acagacgact gagatggacg ttaatcattt ccttgaccac cgcatgcgtc aactcggtgc
     2821 agaaggggca tcatttgagt tcatcgtggc atctggctac cgctctgcta tgcctcatgg
     2881 aagagcgtct gaaaaggtta tccaagctgg tgagaccttg acgctggatt ttggttgcta
     2941 ttaccagcat tatgtcagcg atatgacacg gaccatccat atcggtcatg tgacagatca
     3001 agagcgtgag atttacgatg tggtcttgcg tgccaataag gctttgattg agcaggcaaa
     3061 ggaaggcgtc acctaccgcg agtttgacgc tattcctcgt gaaatcatca atgaagcagg
     3121 ttatggtgcc aatttcactc atggaattgg gcacgggatt ggtttggaca ttcacgaata
     3181 tccgtacttt ggaaaatctg acgagccaat caaggctggt atggtcttga cggatgagcc
     3241 tggtatttat ttggatgata agtatggtgt ccgtatcgaa gatgacctct tgattacgga
     3301 aacaggctgt gaagtcttga cattggctcc aaaagaattg attgtcattt gagaaatttg
     3361 acagtttatg gtaaaataaa acgaataaat aaaattttga agaggtattc aatacaatga
     3421 ttgaagcaag taaacttaga gcaggcatga ccttcgaaac ggaaggtaaa ttgattaagg
     3481 tcttggaagc gagccaccac aagccaggta agggcaacac catcatgcgt atgaaattgc
     3541 gtgatgtgcg tacaggttca accttcgata caacttaccg tccagatgaa aaatttgaac
     3601 aagctattat cgaaacagtt ccagcacaat acctatatca aatggacgac acagctttct
     3661 tcatgaacac tgaaacctat gaccagtatg aaattccagt agcaactatc aaggaagaat
     3721 tgctttacat cttggaaaac tcagatgtga aaattcagtt ctacggaaca gaagtgatcg
     3781 gtgttactgt accaacaact gttgaattgg tcgtagcaga aacacagcca tctatcaaag
     3841 gtgcgactgt tacaggttca ggtaagcctg caactatgga aactggtttg gtggttaacg
     3901 taccagactt tatcgaagca ggtcaaaaac tggttatcaa cacagcagag ggaacttacg
     3961 tatcacgtgc ttaagggttt cctcctagaa aggagagctt atgacaacag aataccaagg
     4021 tgaaatcgtc attgcaccac gcgttcttga agtgattaca ggtattgcag cagccaaagt
     4081 ggaaggggta cattctcttc acaacaagcg tgtggcagat acttggtcaa aatcttcctt
     4141 aaataaaggt gtataccttc aaactgacga cgaaggaaaa gtaacagcag acatttatgt
     4201 ttacctagag tacggtgtca atgttccagc tgtatctatg gatattcaac gtgcagttaa
     4261 aacagctatt tataactacg cagaagttga agtgactgct gtcaatattc atgtcaatgg
     4321 cattgttcca gataaaacac caaaaccagc cttgaaagat ttattcggag aggatttcct
     4381 tgatgaagaa taatagacac gccctgcgta agtgtgcttt gcaggcaatc gtatcacttg
     4441 aatttggtca agaacctgtt caggcagctc agttttctta tctttatgat agagaagaag
     4501 agcaagaagg agttgaaatt ccgctctttc tccttaacct tgtcaatggt gtagcagact
     4561 atcgtgatga cttggataag gagttatcta gccgattaaa agcaggatgg accttagacc
     4621 gtttaacctt gattgacaag aatatcatgc gactaggcct atttgaaatc ttacatttcg
     4681 aagaaacgcc agaccgcgtt gctgttaacg aggctattga attggctaaa gaattttctg
     4741 atgaatcttc agctaagttt gtcaatggag ttttgagcca gtttattaaa gaagaagcct
     4801 gatgagcttc ttttttactt gagaaataag agaatgtatc acaaaaaagc tgcacctaat
     4861 ttttattagg tacagctttt tttatatact agctcctgct agtaggctaa tcggtaggat
     4921 atagtctgtt tttgtcagtt tttttccttc gattttatca actaatagtt caaccagcag
     4981 gcgagctatt tcggttaatg gttgcttgat ggttgtcagt tgaggatagt agttttcgat
     5041 gaaactggtt ccatcataac caatcaattt aatatcttct ggaatagaca gtttcattga
     5101 acgaatgacg ttccatacca gcatagccgt tgcatcgcca gatacaaaga taccatctgg
     5161 tttttgcttt tcaagaatag ctcggatttc catctccttg cgcacaggtg agaaatcact
     5221 ggagatatga aaaacttcgg ctttgttcaa aatagatgtg aaacctactt ggcgaaggcc
     5281 ggtaggtgaa ttggaatcat cgttccccgt aatcattaat ggattgtgac atcccgcttt
     5341 ttgtaaggtt tgggcagcta ataccccgcc agcaaaatta tcggaagaga caatggggat
     5401 ggaaggagag aggttgcggt caaaagcgat aattggagct acgacacggt cgtagtcttg
     5461 gataccgaga ttgtgactgc ccgagataat cccatctact tggttagctg ctaacatttc
     5521 tagatactca cgttccttat cggactcgtg ctggctattg caaataatgg ttttatagcc
     5581 tcgatcaaaa agctcttttt ctaaatggct aatcaattca gaataaaaaa tattgtttat
     5641 tgttgggaag atgagaccaa ctaattttgc agatttacct tgcaaactac gagctaaatt
     5701 attaggtttg tagccgagtt cacgcatagc ctcttcgaca ttgcgaatgg ttttttcaga
     5761 taggtatcct tttttattga taacgcgcga gacagttgtt gggctgaccc cagctaactg
     5821 tgcgacatca gttagttttg cgaccataat ctaaatggta gtaggttccg gtcggattac
     5881 cagtcgtaat gacgatacca gactgatccg catcagggaa gacacgacca gaaaatactt
     5941 tctctccttt attgataaag atttcaaaga cagatttgtc gatgaagata ttggctgtga
     6001 gagctcctgg agaaatctcg caagagcgac ttgtaccgaa atcgagtgcg aatggctgac
     6061 cacagttgcc acggtccaat gtaatctggc cttgctcagt gtccacagac aagaccaagc
     6121 ctttttcttc ttcatttgcg aacaatacga tttcatgctt tgtgcccgct gaaaaatcaa
     6181 gttccaattc gtaaacatta ttggtttctt tttgggctga aaatggctga gcttctttac
     6241 gaagactggt aatcgcattg actggatatt ggtagagctt accgtccttg atggttaatt
     6301 ctttaaccaa tgacagacag ccttggtagt cgtaagcatc ggttgggtat tccacatctg
     6361 gaagtgccaa ccagctaaca gatagagttc gaccgtctgg tgcattaaag gcctgggttg
     6421 catagcactc aaaaccatag tccagattga tcaattcgct cggttcgaca atgcttgctg
     6481 tttcaggatc gaaactctga ccaatcttgt acatatttgg atagatattg ccatagtcca
     6541 atacctcttt gtccaagcct tgtggacaat gaagaaggat gggttgctca ccgacgaacg
     6601 caatatttgg gcattccatc atataagcgg tcatatcatt ggcaaaatct aggtcaccaa
     6661 cataggacca tttgaggtag tcgttatttt cagctttata aagtttgaca attccctttt
     6721 tatccagatt ttgtccaccg acgatagcgt aatattgccc tttatagttg aaaatttgtg
     6781 gatcacggaa atggtcggtg gtatcttctg gttgctcaat caagacttta tcaatctttt
     6841 caatcttacc atctttatcc attaaggcac cgatttggta tggatggcga acccaatttt
     6901 catcgcggac atttccagta tagaagagga agagcttgtc gccaaattcc atggcagatc
     6961 cagagtaggc accgtggcta tcaagtggtg tatctgggta cataaccgta ccagtttctt
     7021 caaaatggac caaatccctt gactctgtat ggacccaaga ttttaatccg tgcgcagcac
     7081 cgaatgggaa gtattggtaa aaaagagtcc actttccatt aaaataagaa aatccatttg
     7141 gatcatttaa cagtccatgt ggtgtttcaa tatggaaatt tgtcctccaa ggagaactct
     7201 ctgtattctt ctttattttt tcaatatatt cgagtgtcca gtcagcataa gctctgtaac
     7261 gctcctctgt tgtaaatgcc ataattttct ccaatttttc tagtttttca ttaatatagt
     7321 aaacgttttc tttcaaaaaa tcaagtttca acaatgtttt tggatgcaat tgaaacaaaa
     7381 tgtgataaac gtttgacata ttttataaaa gtgatataat aaaaccatcg aaagtttgaa
     7441 aacactttca aaaaattaaa aaatatacaa aaaggagatt tttgtaaatg gattataaaa
     7501 aagtttctgc cgaagtgatc gaagcggttg ggaaagataa tctagttgcg gcagcacact
     7561 gtgctactcg tcttcgtttg gttctgaagg atgaatccaa agtgaaccaa aaagccctcg
     7621 atgacaatgc agatgtcaaa gggacattca gtacgaatgg tcagtatcaa gttatcatcg
     7681 gacctggtga tgtaaacttt gtatatgcag agctcatcaa ggctacaggc ttaaaagaag
     7741 tttcaacaga tgatttgaaa cagattgcag cgcaagataa gaagttcaat ccattgatgg
     7801 acttgatcaa gctcttgtca gatatctttg tgccaatcat tccggctctc gttgctggtg
     7861 gtctattgat ggcgcttcgt aacttcttga cttccccaga tttagttggt caaaaatcaa
     7921 ttgaggaaat gtatcctgct atccaaggca tttctgcgat gattcaattg atgtcagcag
     7981 ctccattcat gttcttgcca gtccttgtag gtatctccgc tgcgaaacgc tttggtgcaa
     8041 accaattcct cggtgcggct attggtatga tcatgaccac tccagatttg ggtggtaaag
     8101 aagctttttg ggatattttt ggtttccaag tagctcaaac aaactatgca tatcaggtta
     8161 ttccggttct tgcagcagta tggcttcttg cgacgcttga aaaattcttc cacaagaagt
     8221 tgccatcagc agttgacttt acattcacac cattattgtc agtaatgatt acaggtttct
     8281 tgacatttac agtcatcggt cctgtgatgt tgactgtatc tgatgcgatt actaacgcaa
     8341 ttgtttggtt gtacgataca actggtgcgt tgggtatgag tatctttggt ggtacttact
     8401 cattgatcgt aatgactggt cttcatcaat cattcccagc catcgagaca caacttttgt
     8461 ctgcttacaa caataatggt acaggatacg gagactacat ctttgtagtt gcttcaatgg
     8521 ctaacgttgc acaaggtgct gcaactcttg ctatctattt cttgacaaaa aatgcaaaaa
     8581 caaaaggttt gtccggttca gcagcagttt ctgccttcct tggaattaca gaacctgccc
     8641 tcttcggggt taacttgaag tacagattcc cgttcttctg tgctcttgca ggttcggcta
     8701 tcggtgccct ctttgcaggt ttgactcatg ttatcgctgt atctcttggg tcagcaggtt
     8761 tcattggatt cctctcaatc aaagcatctt ctatcccaat gtatgtagtt gctgaggtga
     8821 ttgcttttgc tgcaaccttt gcctttactt acgtttatgg caagacaaaa gcatcagcag
     8881 ttttcgctga tgaagcagca acagcagctg cgacagcagt gacagcgtct gtagttgaaa
     8941 cttcagaaga agttgaagaa actgcagaaa tctttactgc acaagaaact cttgtatctc
     9001 cactttcagg tgacgttgtt gcccttgaaa atgttaatga cccagtcttc tcatcaggag
     9061 ctatgggtaa aggtttggct gttaaaccat ctgagggtgt tgtttacgct ccagcagatg
     9121 cagaagtaac aattgctttt gaaacaggcc atgcttacgg attgaaaaca gcgtatggtg
     9181 ctgaactttt aattcacatc ggtattgata ctgtatcaat gaacggtaat ggatttgaaa
     9241 aattagtagc agcaggcgat aaagtaaaag ctggtactcc aatcgctaaa ttcgatgctg
     9301 cgaaaattgc tgaagcaggt ctggatgata ctacgatggt tatcgtaaca aatacagctg
     9361 attttgcaga agtatctcca cttgcagagg gagcaattgc tcacggagca aacttcttga
     9421 aagtagctaa ataagaatca taatccgatt ttaaggaatc ttaattttat tgaacaagga
     9481 gtaggaaaaa ggcggaatag cctttttcct ctgtttgttt ctatggtata atgctagtga
     9541 taaaattttt ggagaaaatc aaatgacaaa attatatggt agtttagaag ctggtggtac
     9601 aaagtttgtt tgtgcagtag gcgatgagaa ctttcaagtt gttgaaaaaa cgcaatttcc
     9661 aacgacaact ccttatgaaa cgattgagag aacggttgaa ttcttcaagc gctatgaaga
     9721 tcgtttggca ggtattgcga taggttcttt cggtccaatt gatattgatc aaaactcaca
     9781 gacttatggt tatatcactt cgacaccaaa accacactgg tcaaatattg atttacttgg
     9841 tctgattgct aaggaattta acattccatt ctacttcacg acagatgtga attcatcagc
     9901 ttttggtgaa acattggttc gtaaaggggt aaagagctta gtttactata caatcggtac
     9961 aggtattggt gcaggtgcta ttcagaatgg tgagtttatc ggtggcttgg gtcataccga
    10021 agcgggtcat acttatgttg ccctacaccc ttatgatgta aaacatgaat ttaaaggaat
    10081 gtgtccattc cacaatgggt gtttggaagg cttagctgca ggtccttctc ttgaaggtcg
    10141 tacaggtatt cgtggtgaat taatcgagct caactcagac gtttgggatg tgcaggctta
    10201 ctacattgct caagcagcag tacaggcaac cttgctttat cgtccgcaag taattgtatt
    10261 tggtggagga gttatggctc aagagcacat gcttaatcgt gtacgtgaga agtttgtcgg
    10321 tttgatgaac gattatctcc caacaccaga tgtcaaagaa tatatcgtaa cgccagctgt
    10381 tgctgaaaat ggttctgcaa ctcttgggaa ctttgctttg gcaaaacagg tgtcaagaaa
    10441 ttcataattg tctatgtgaa cagattcatt tgtactttgg cgatagatat attgaagaag
    10501 aatattgggt gaagttgttt tattatcaca ataacttgac agtgaggtga gagtttttat
    10561 gtaactctca cctctttata cctaaaaaac tgtattcata agagatagcc gtttgttaat
    10621 caaggcagtc tttgggagta ggaaagaatc agaccggctt aaaaagagtt cgtcaacaac
    10681 tcttcaattt aagtttttga gccgaaacag cgcctcaaaa aactaacgat gactataaaa
    10741 gattagagtt tgccattgtt ggagttcatc gggtatgaaa aagagaaacg agttctacaa
    10801 ttcttgatga ttattcataa gcaaggttga aaatcttttt attagcgata gtgcagtgat
    10861 attttttaac cagaggaaag ttgttgtttc ccaattgtat gaaaacgtta tatctaaagt
    10921 aatgatgcgg agacactaaa gtctataaaa acaggaaaag cacagataga tagcagaaaa
    10981 aagatagtta aaagtgttac aattgggatg caaattttag gctgggaaat tattctcaga
    11041 agtatttata tggaggactt atgattccaa ttcaacagca tggtaagcgc tggcttattt
    11101 tgacacttac cctttctact tcggttcttg cgcttgcatc tatcggagca tcacaaccgg
    11161 ttgtttttgc agatgaaaac tcacatttac aatcacaaga aaataccaat aaaatagagg
    11221 ttctggactg ggaagcattt tctaaaaaat tgaaagacta ctcctcggac caacgacaat
    11281 ttcatgtttt gaaattggga tttgaaaatc gtcttgggac attatcgact cgggaagaat
    11341 tggaagaatt tggtaaaaac aacaattttt tggtaatcaa tggtaaagtt actcaaaata
    11401 ttcatgactt tccacatatc cttgttatga acaagggtga tgttattgcc cataatgagg
    11461 aagaatatgc aagcttaaca aaagatttac ggtttgcagg ggattataag agtacagtta
    11521 gtgatattgg ataccatcct cagagggttc atgctttatt ccaaattcaa cttgactcac
    11581 ataaaagacg attgttagag gcggcagggc tagggactgc tgataacagc atgagaaacg
    11641 taaatggctt taacatatat tctcatgggc tctctattga taacaaatat tatgcaaatt
    11701 atggcaactt taataagtat gataatctta atgttactag agaacgtttt gataaaaatg
    11761 atcaaacgat tcaaaaatta atcagagatt caggcgcttt ttcggaacaa ttagatcgtg
    11821 aaaaaattaa gacttttgct gtatatgtag ctaatcgtgt tacatatgat tggactgctg
    11881 ctaataaagc agttaccaat caagcggatg taaattacta tttgggttca gatttatttg
    11941 cggttacaga acagcaaaaa gccatgtgtg ttgggttcag tactacggcc gcccgtgctt
    12001 tcaatatgat ggggatacct gcttatgttg tgactggaaa taatagatcc ggtgtgcctc
    12061 atgcaacgac cagagcttat tatgatggta aatggcatac tattgatgct actggtttta
    12121 taaatggtac ggtaagaaat tcagctattt atagttcaca tcactttgac acaatcggag
    12181 aggatagtta tgaggtggta gataaaggga agttagaaga gcttcctctg aatagcaaga
    12241 actacatggt tattgataaa gaatatgaag aatgggcgaa gaaacaaaag gcagcagatt
    12301 tacttttagt taattctgat aaggcttttg ttgcaaaaga atatatagct tatattgaac
    12361 caacatatgt gtcacaagaa aataaaaaac atttattaga catttacaaa gttttgagag
    12421 aaaaaataga agaagcaaag gcaaaagatt taaattcgga acataaaaat ggctacgaat
    12481 ctattttgaa aacagctgta tctgatattg acaagttgaa taattctgta aatatcacgg
    12541 aaaaagaatt tgatgaaatt aaaaattcta tgaaaaataa taaaggtttc ttatcgcaat
    12601 tagataaaga tgctgcagca agttttgaag acggaaatga ttataaaact tatctggcaa
    12661 taatggatgg taaaaagctt caagaagatc ctaccattat caaagacaat cacaatgttt
    12721 ctgaaaataa attaagtcct ttgaaggaag aaaaccaagc taattctgat gaagtcagcc
    12781 aaaattccaa ggatgcttcg gctccaagtg ttaattctgc acagcaatca gaggagcttg
    12841 aaggaactac ttcaacacag gaaacaatct cagctgctcc ttctcaacaa acaccagctc
    12901 ctaaggcact acaagctaaa actgagttag aggataaaac agagacttca tctagttcct
    12961 ttgagaaaga atcgaatgaa gtgtcggcta atatagtttt gtctcctgaa ataactaaga
    13021 acacgggaag tctagcttct gtagaatctc gagtggagga ggctgtggat gggacaactc
    13081 catcagaaaa agtttctgct gaatcagaag aagtagaaaa aacaattgta tctgaggtat
    13141 catcggatgc ttcattagca aatgagaaca aaaattctga tgctcaagaa gatactccac
    13201 gtttggagaa atctgccgag gtggtaacaa ctagtcaaga agtagctgaa gcaccggttt
    13261 ctgctccaca agtaagttct gaaatcccaa cttcatcaga aacagctcct acagaggtgg
    13321 caacagaagc accggaatta agtgcacttg agagtaatcc agcacctcaa acttcattgg
    13381 aaacaactcc tacagaggag gtaacagaaa caccggaacc aagtgtagtt gagagtaatt
    13441 cagcatcccc aacttcaccg gaaactaatt ctgcagaggc agtaacaact agtcaagaaa
    13501 tggctggaac atcggtttca gctccacaag taatttctga aatcccaact tcatcagaaa
    13561 cagctcctgc agaagtggca acagaagtaa cagaaccaag tgtagttgag agtgattcag
    13621 catccccaac ttcaccggaa gttaattcta cagaggcagt aacaactagt caagaaatgg
    13681 ctggaacatc ggtttcagct ccacaagtaa tttctgaaat ccaaacttca ccggaaacag
    13741 ctcctgcaga agtggcaaca gaagtaacag aaccaagtgt agctgagagt aattcagcat
    13801 ccccaacttc accggaagtt aattctacag aggcagtaac aactagtcaa gaaatggctg
    13861 gaacatcggt ttctgttcca caagtaattt ctgaaatccc aacttcatca gaaacagctc
    13921 ctgcagaagt ggcaacagaa gtatcggaag agagtagttc agcatctcaa ccttcatcgg
    13981 aaactaattc tacagaggca gtaacaacta gtcaagaaaa agctgactca ctggtttctg
    14041 ctccacaaac aagttctgcc tcacttactt tagaggttcc taaagctgaa catttagacg
    14101 agaaagcaga tgcaactatg ccaaatggag ttgaagcaaa tactcatgag gcaggtgctg
    14161 ttccgacatc agatatttat gttcaagatg ctggttcaga cacggtgcct caacctcagt
    14221 ctttatctgc tacagtactt agagaagcaa tttcaacagt tgagcctgta agcgttgcaa
    14281 cttcttctca agagagaagt gcagtagctg gtgaggagaa agtgcctgcc tctcttgaac
    14341 ctagtaacaa ttctgttaag gaagaaaaag tggtagattc aaatacaaca attgagaatc
    14401 aagagacaga gaaaaaagag gtccttactt cggaaaatgt acttaatagc ttagtaacaa
    14461 tttgggtagc tttatctact agtttcttca tgagatattt ttctcgagga aaataatata
    14521 ttaacacagg ttaaagatga cgtttatctt tgtgtagaaa attattcatg tgagctttta
    14581 ttaaccgaat aatatgacaa ctgagctttt aatgaatttc taaatctcca atatatcaca
    14641 gattcctcat ttaaaatttt ataaatgttt cacctcctag gagttattgt gactaggagg
    14701 tgttttgtgg taaaaatagt aatattaagc tagcattttc atttatttag gctgtatcct
    14761 agtgccaaac cttgaaaatt gcctagtttt ctgctacaat atattattga ctaaaactga
    14821 aataggatga atgatatggt aacaaatgta cttcgttctc tcattgaaaa tgataaggga
    14881 gagctaagaa aacttgagaa aatggcagac aaggtcttct cgtatgccga agaaatggaa
    14941 gcgctgactg atgaacagtt gcaagctaag acagcagaat ttaaggagcg ctacaataac
    15001 ggtgaatccc tcgacgatct tttgtacgaa gcttatgcgg tagtccgtga aggagctcga
    15061 cgtgtcttgg gtctctatcc ctacaaggtt caggttatgg gaggaatcgt tcttcacaac
    15121 ggtgacgttc cagaaatgcg tacaggtgaa ggtaaaactc tgactgcaac gatgcctgtt
    15181 tatttgaatg ccttgtctgg tcaaggtgtt cacgtagtga cggttaacga gtatctttct
    15241 actcgtgatg cgactgaaat gggtgaattg tactcatggc ttggtctatc agttggtatc
    15301 aacttagccg ctaaatcacc cttggaaaaa cgtgaggcct ataactgcga tattacttat
    15361 tcgaccaact cagagattgg ttttgactac ctccgagaca atatggttgt tcgcgcagaa
    15421 gacatggttc aacgtcctct caattatgcc ttggtcgatg aagttgactc aatcttgatt
    15481 gacgaagctc gtacgccgtt gattgtatct ggtgcacaag gttctgaaac aaaccagttg
    15541 tatttcttgg cagataatct tgttaaatct ttgacaacag aagattacat cattgacatt
    15601 ccatctaaaa cgattggtct gtctgattca ggtattgaca aagcagagaa attcttcaag
    15661 ttagataacc tctacgatat tgaaaatgta gccattaccc atttcttgga taatgcactt
    15721 cgtgccaact acattatgac ctatgatatt gactatctgg tcaatgaaga ccaagaggtc
    15781 atgattatcg atccatttac aggtcgtacg atggaaggtc gtcgttattc tgatggactt
    15841 caccaagcca tcgaagctaa ggaaggtgta ccggttcaaa acgagtctaa gacaagtgcc
    15901 tctattacct accaaaacct tttccgtatg tataaaaaat tatcagggat gacgggtact
    15961 ggtaaaacag aagaggaaga attccgtgaa atctacaaca tccgtgtcgt accaatccca
    16021 accaaccgcc caatcgcgcg tgttgaccac gaagatttac tctatccaag tctagaatac
    16081 aaattcaatg ccgttattgc tgatgtgaag agacgctatg agaaagggca acctgtcttg
    16141 gtcggtacag ttgcggttga aacttctgat ttgatttctc aaaaattggt agctgcaggt
    16201 gtccctcacg aagtattgaa tgctaaaaac cactatcgtg aagcgcagat tattatgaat
    16261 gctggtcagc gtggtgcggt cactattgcg accaacatgg cgggacgtgg taccgatatt
    16321 aagttaggtc caggtgtacg tgaacttggt gggctatgtg ttattggtac tgagcgccat
    16381 gaaagtcgtc gtattgataa ccagcttcgt ggacgttcag gtcgtcaagg tgacccaggt
    16441 gaatcacaat tctatctttc tctcgaagat gacttgatga aacgctttgg ttcagaacgc
    16501 atcaaagtat ttatggagcg tatgaatcta acagaagaag agtccgttat caagtctaaa
    16561 atgttgacac gtcaagtcga atctgctcag aagcgtgtgg aagggaacaa ctacgactca
    16621 cgtaaacaag tccttcaata tgacgatgtg atgcgtgagc aacgtgaaat catctatcgc
    16681 caacgccagg atgttattac tgctgaccgt gatttggctc ctgaaatcaa agcgatgatg
    16741 aaacggacga ttgaacgcca agttgctggt cacttccttg gttcgaaaga tgaagctatt
    16801 gatggaatta tcaaatttgc gcatgcaaat ctcgttgaag atgatacctt gagtaaagct
    16861 acttttgaag caatgaatca gaaagagatt gtcgaagagc tctatgaacg tgctttgaga
    16921 gtctatgatt cgcaagtgaa aaaattacgc gatgaagaac gtgtacgcga attccagaaa
    16981 gttcttatcc ttcgtgttgt ggataacaaa tggacagacc atattgatgc cttggatcaa
    17041 ttgcgcaatg cagtcagtct tcgtggatac gctcaaaaca atccaatcgt tgaatatcaa
    17101 tcagaagcat ttactatgtt caacgatatg attggggcta tcgagtttga agtaacccgc
    17161 ttgatgatga aggcgcaaat tcatgacaat attgagcgtg agcgtacaag tcaagaagca
    17221 catacgacag ctgttaagaa tattatgccg aaccaatcgc atgcaattca ggagaatgta
    17281 tcttttgaag gtgttgatcg taacgatcca tgcccatgcc agtctggtaa gaaatttaaa
    17341 aattgtcacg gccgtaaata ggaatgacac gtggactgga cggctgtcca gtccttttgc
    17401 aatttttaga caagtcacta gagtcatttc gtttatttta ataaatgaaa tgactatgca
    17461 aaaggagacc gattaatgat agtaggacat ggcattgacc ttcaggagat ggaggcgatt
    17521 gaaagtgcca gaatcaaaca ccaagggttt cccaaaaaga ttctcacaga gaaggaattt
    17581 gaacggtatc aaagtctgtc cggtcgccgt cagctagaat atttagctgg acgttggtca
    17641 gccaaagaag cgctgaccaa ggccttgggg acaggtattg gtaagatagg atttcatgat
    17701 attgaaattc tgaataccag caaaggagtt ccttatgtga cgaagagccc ctttgatggg
    17761 aatatttggc tcagcattag ccattcagga aattttgtac aagctagtgt gattttggag
    17821 gagaaagatg attgaaagtg aacatcgccc cacgcaaatt cgggttaact tagatacgat
    17881 tgcggaaaat ttcgagcaag taatgaccaa cttgtcccct aagactgaag catttgctgt
    17941 tgttaaggcg aatgcttatg gtcatggagc tgttgcggta gcgaaaaagt tatccagtca
    18001 agcagctggt ttttgtgttt cgaatttgga tgaagcatta gagttacgta aagcaggtat
    18061 tgagcatcct atcttgattt taggagtggt tcctgtacgt tttttaccag tagctcatca
    18121 actcaatatc agtgtgacag ttgccagtct ggactggttg caagatgcaa aagacttaga
    18181 ggctgattta tctggtctaa ctgttcatct taagctagat acaggtatgg ggcggattgg
    18241 ttttagaaaa atagccgatt tgctacaagc gattgctatc cttgaagaat tagagtatga
    18301 catcgaagga gtttatactc attttgctac tgcggatgag gttaatcagg cgcacttcga
    18361 gagtcaactg tctgtttttc aagaattttt ggatgttcta ccaattactc cgcgctggat
    18421 tcatgccagc aattcagcaa caagcatttg gcatgctgat acagtattta acctggttcg
    18481 tttgggaaac attctttatg gacttaatcc aagtggacga gtgctagaac tcccgtttga
    18541 tgttcaacca gccttatctc tcgtatctga aattgttcat gtaaagcagg ttgaagctgg
    18601 cacaacaatt ggctacggag ctacttatca cagcgtcgat tcagaatgga ttgcgaccgt
    18661 tccgatagga tatgcagatg gtctcgtacg aagcttacaa ggcttatcag tcttagtcaa
    18721 tgggcaggcc tgtgaaattg tagggcgtat ttcgatggac cagatcacca ttcgtttacc
    18781 tcatgcttat ccgcttggtc aaaaagtcac cctaattggt caagatgagg atcaaactat
    18841 ctctgttcag gagtgggcgg atcgcatcgg aaccataaat tacgaggtgg tttgtctttt
    18901 gacagatcgc ctacctcgaa tttttgaata acagcgatag taaaagtgac gataatccgc
    18961 ctctttcact agaatagaag gctaaaatat tagagaagtg ttacctagat attgtctata
    19021 atacaaatat cgtcatttag ttgac
//
LOCUS       DATUCG010000033        18998 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.034, whole genome shotgun sequence.
ACCESSION   DATUCG010000033 DATUCG010000000
VERSION     DATUCG010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18998
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.034"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(75..587)
                     /gene="tadA"
                     /locus_tag="VCA20_000904"
     CDS             complement(75..587)
                     /gene="tadA"
                     /locus_tag="VCA20_000904"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEP1834751.1"
                     /translation="MNYTLEEKEYFMRQALEEAEKSLEKNEIPIGCVIVKDGQIIGRG
                     HNAREELNQAIMHAEVMAIQEANRSEGSWRLLDTALFVTIEPCVMCSGAIGLARIPQV
                     VYGATNQKFGAAGSLYDILTDERLNHRVEVETGILEEDCAQIMQDFFRQRREKQKADK
                     LATKSQVDQS"
     gene            complement(640..2610)
                     /gene="tkt"
                     /locus_tag="VCA20_000905"
     CDS             complement(640..2610)
                     /gene="tkt"
                     /locus_tag="VCA20_000905"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEP1834752.1"
                     /translation="MSNLSVNAIRFLGIDAIEKSKSGHPGVVMGAAPMAYSLFTKELR
                     INPAQPNWVNRDRFILSAGHGSMLLYGLLHLSGFEDVTMDEIKNFRQWGSKTPGHPEF
                     GHTAGVDATTGPLGQGISTATGFAQAERFLAAKYNRDGFPIFDHYTYVICGDGDLMEG
                     VSAEAASYAGLQKLEKLIVLYDSNDINLDGETKDSFTEDVRARYNAYGWYTDLVTDGT
                     DVDAIFAAIEKAKVAGKPSLIEIKTVIGHGSPNKQGTNAVHGAPLGAEETEATRKALD
                     WNYGPFEIPAEVYADFKANVADRGAAAYDAWVKLVEDYKVAHPELAAEVTAILEGRDV
                     VEIKPEDFPVYENGFSQATRNSSQDALNAAAKVLSTFLGGSADLAHSNMTYIKEDGLQ
                     DAATPLNRNIQFGVREFAMGTILNGMAAHGGLRVYGGTFFVFSDYVKAAVRLSALQGL
                     PVTYVFTHDSIAVGEDGPTHEPIEHLAGLRAMPNLNVFRPADARETQAAWYLSLTSKS
                     TPSALVLTRQNLTVEEGTDFDKVAKGAYVVYEAAGFDTILLASGSEVNLAVKAAKELE
                     AAGTKVRVVSVPSTELFDAQDAAYKEEILPNAIRRRLAIEMGATQSWYKYVGLDGKVL
                     GIDTFGASAPAQTVIDNYGFTVENVVKLVGEL"
     gene            complement(2822..3913)
                     /gene="ulaG"
                     /locus_tag="VCA20_000906"
     CDS             complement(2822..3913)
                     /gene="ulaG"
                     /locus_tag="VCA20_000906"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEP1834753.1"
                     /translation="MAKVQDITRESWILSTFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     NCGVWIKTPGGANVVMDLWSSRGKSTKKVKDMVWGHQMANMAGVRKLQPNLRVQPMVI
                     DPFAINELDYYLVSHVHSDHMDISTAAAIINNPKLDHVKFVGPVESGDIWEKWGVPAD
                     RIIRIAPGDSFEFKDIKVHAVESFDRTCLVTLPIEGYDENGGKLAGLPVTDELMARKA
                     VNYVFETPGGTIYHGADSHFSNYFAKHGRDFNIDVAINNYGDNPIGIQDKMTSIDLLR
                     MAENLRAKVIIPVHYDIWSNFMASTDEILQLWKMRKERLQYDFHPFIWEVGGKYTYPL
                     DKDRIEYHHPRGFDDCFLEDSNIQFKALL"
     gene            complement(4023..5711)
                     /locus_tag="VCA20_000907"
     CDS             complement(4023..5711)
                     /locus_tag="VCA20_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEP1834754.1"
                     /translation="MLLDKSSCALLRYLIGLQEPETIMAISKGLQQSRRKIYYHLEKI
                     NDALPTTVESIESLPRLGIRLTEAQKLACRDLLDSIDSYTYVMSMPERIQLMLVYICI
                     TPERVTIERLMELTEVSRNTVLNDLNEIRTQLAQEQYKMTLYVSKSQGYSLVCHPLNK
                     IQYVHSLLYTIFLDANKGFLQVLEDKLFELVGCQLLFSEEMAQFLQGQVSQLEKKLGK
                     KINRYEIEFMLKVLPFLLLSYRNMELDDLEERAILKEFEQVRERIEYRVAQELKDALL
                     QTFDLKLDEIEISLIAILLLSYRKDSDVHVFSQDFIDLKRVVERFIHHFEIHSPFELE
                     NPDELAKHLLAHCKALLFRKTYGILSKNPMVQQIQDKYGTLFAMTRVSGKILEEEWQI
                     ELSDGDIAYLTIHLGGAIRRSGTRKAQTQIVYLICDEGVSVQRLLYKQCQHHLPDKKI
                     GAVFTTEQFKSVEDLLEVDFLITTSDGLETDFPLVQVHPILDFDDVLNMTHFAKYRSL
                     ADEKQVFAVELDKLLSGYIADGRTAQELKQRLQKLITNELLTNLSSQDIETDLY"
     gene            complement(5836..6453)
                     /locus_tag="VCA20_000908"
     CDS             complement(5836..6453)
                     /locus_tag="VCA20_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834755.1"
                     /translation="MRKFLQDSLTETAYYLELILSAILGLALLALSTLLFADLLSSLA
                     GGIQADTFIQNFLSRAMTLAVGVEFIKMLCKQSPSTVIEVLLVAIARQLIVEHGSSSD
                     YLIGIISVAILFAVRKYLFTQFDDSNNIIMRASQKVKVANIIARVKIPSEGSETLRDF
                     VTRKLNEEEKNIAIGACVYLKNVALRIDNMHGELITRVEIIKSIY"
     gene            complement(6539..7255)
                     /locus_tag="VCA20_000909"
     CDS             complement(6539..7255)
                     /locus_tag="VCA20_000909"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001934215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEP1834756.1"
                     /translation="MPKDLQEMRQRVYEANIALPAHGLVKFTWGNVSEVCRELGRIVI
                     KPSGVDYEKLSPENMVVTDLDGNVVEGDLNPSSDLATHVALYKAWPNVHAVVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGPVPCARSLTAEEVNSAYEKETGSVIIEEFERRGI
                     DPVTVPGIVVRNHGPFTWGKDPAQAVYHSVVLEEVARMNRYTEQINPRVEPAPSYIMD
                     KHYLRKHGPNAYYGQKGDAH"
     gene            complement(7255..8118)
                     /locus_tag="VCA20_000910"
     CDS             complement(7255..8118)
                     /locus_tag="VCA20_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEP1834757.1"
                     /translation="MARPIGIYEKATPKHFTWKERLEFAKELGFDFVEMSVDESDARL
                     ARLEWTKEERLDLVKAIYETGVRIPTICFSGHRRYPLGSNDPALEAKSLETMKQCIEL
                     AQDLGVRVIQLAGYDVYYEEKSPETRERFIKNLRKACDWAEQAQVMLAIEIMDDPFIN
                     SIEKYLAVEKEIDSPYLFVYPDTGNVSAWHNDIYSEFYLGHRSIAALHLKDTYAVTEN
                     SKGQFRDVPFGQGCVDWENMFAVLKKTNYQGPFLIEMWSENCQTVEETRAAIKEAQDF
                     LYPLIEKAGLN"
     gene            complement(8124..8786)
                     /locus_tag="VCA20_000911"
     CDS             complement(8124..8786)
                     /locus_tag="VCA20_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014622185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEP1834758.1"
                     /translation="MKHIPNLQVALDHSDLQGAIKAAVSVGHEVDVIEAGTVCLLQVG
                     SELVEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICSATIPTMAAALKA
                     IKEVRGDKGEIQIELYGDWTFEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNKV
                     KKLVDMGFRVSVTGGLDVDTLKLFAGVDVFTFIAGRGITEAADPAAAAREFKDEIRRI
                     WG"
     gene            complement(8797..9282)
                     /locus_tag="VCA20_000912"
     CDS             complement(8797..9282)
                     /locus_tag="VCA20_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEP1834759.1"
                     /translation="MNLQKSFTENNSIRLGLTAETWQEAVHKAVDPLIDSGAATEEYY
                     DAIIASTEEYGPYYVLMPGMAMPHAQAGVGVLKDSFALITLTKPVVFSDGKEVSVLLT
                     LAATDPKIHTSVAIPQIVALFELENSIERLIACQTPEEVLAMVEESKNSPYLEGLDLE
                     S"
     gene            complement(9344..9622)
                     /locus_tag="VCA20_000913"
     CDS             complement(9344..9622)
                     /locus_tag="VCA20_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEP1834760.1"
                     /translation="MVKVLTACGNGMGSSMVIKMKVENALRQLGVTDFQSASCSVGEA
                     KGLAAGYDIVVASNHLISELEGRTKGHLVGLDNLMDDNEIKTKLQAVL"
     gene            complement(9681..11129)
                     /locus_tag="VCA20_000914"
     CDS             complement(9681..11129)
                     /locus_tag="VCA20_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEP1834761.1"
                     /translation="MDFLQTPLNWFSQNILQNPAFFVGLLVLVGYALLKKKPHDVFAG
                     FIKATVGYMILNVASGGLVTTFRPILAALNHKFQIDAAVIDPYFGLAAANGKIAEEFP
                     DFVSAATTALLIGFGVNILLVALRKVTKVRTLFITGHIMVQQAATISLMVLFLIPALR
                     NQFGTAAIGIICGIYWAVSSNMTVEPTQRLTGGGGFAIGHQQQFAIWFVDKIAPKLGK
                     KEENLDNLKLPSFLNIFHDTVVASATLMLVFFGVILFILGPEIMANPEVITSGAPYNP
                     AKQDFFMYVIQTAFTFSVYLFILMQGVRMFVAELTNAFQGISSKLLPGSFPAVDVAAS
                     YGFGSSNAVLSGFAFGLIGQLITIVLLIVFKSPILIITGFVPVFFDNAAIAVYADKRG
                     GWKAAAILSFISGVLQVALGAVCVGLLELSGGYHGNIDFEVPWLPFGFAFKYLGIAGY
                     VLVCLFLLAIPQLQFAKAKDKEAYYRGEAQAE"
     gene            complement(11450..17656)
                     /locus_tag="VCA20_000915"
     CDS             complement(11450..17656)
                     /locus_tag="VCA20_000915"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pullulanase"
                     /protein_id="HEP1834762.1"
                     /translation="MKKKSVQNMKRTGMLEKKQIFGIRKLNVGVASVGIAAALFLSGA
                     GQLVQAEEVVLPASTSSTTLSEQDGGAVEATATAIEATDSMVAEATAVTETADSTASE
                     EAPIEEGSIRLHFENVDEIAPESQGLWTWGGVAEPSDGNQWPTDTANFSSSQVDDYGH
                     YVDIKKSETPGTIGYLVLKDGEKITESDQKVELLVPEQNEAWIATDYTVSSYEPLKDE
                     NILRINYTREDNNYEGWGVWTWGDTTEASDGWPTGAVDFKLGKYGAYVDIPLSNGLDS
                     KLGFLLINQNNPDLAGNKTIDLAFADRKRHSQIFLRNDDDKVYINPYFIEEKVELDTS
                     KATPGTKNVTLEASVKAPFNYNESGLVSVTVTNPENAEIVKMEVDTSAIGGGLVPIST
                     ELNRVTITERIKADGEKDWDEQVIYFMMTDRFYNGDTSNDKLVEGTASNPRGLYQGGD
                     FKGVTAKLDYLKELDVDSIWLTPIVENIPQNVGSVKDGEYYAYHGYWASNFEKLNPHL
                     GSLADFHELIDAAAEKGINIVVDVVLNHAGYGTEETFAGMVRTKEEDKQGDDQLGSLS
                     GLPDFKTEEAAVRNQLVAWQASWLERSTTAKGNSIYAFRVDTVKHVDDTTWQHFKNEL
                     VDRDPDFHLIGETWGANYKDTKGDLGIGTMDSLLDFGFKDIAKYLVNGQLKAAGKELE
                     ERSKVLTSAASLGQFLGSHDEDGFLYSLGGAEKEGNLDKLKLAASLLITAKGQPVIYY
                     GEEVGQSGQNNWPVYDNRYDFDWSKVETSDIEDHYQKLLAFRNANSTLLSRGDTTTLA
                     GNDSQGWLISKRSYQDQAAYLVFSTNTENQEMALEVSGKDVVVKDAYTGKSYQAIEKD
                     GKWVVQVELPTIAQGGTMLLQTEAGDIVNASVQGATEEPIEAGYFRVHFKTLPSDNLS
                     SLGLWTWDDVEKPSSDLGAWPTGATNFSTAKQDDYGYYLDIKMKDETASKISLLINNT
                     SGDNITGDKTIERISTKMNEAWFDENYQLSLYQPLKEGYIRINYFRTDGNYDQKGLWI
                     WGDVTDLTLGDWPNGIDFENQGKYGAYIDVKLTDLPSSIGFLLLDESKSGDDVKIQQK
                     DYSFKDLKNQTQIFLKDDDATIYTNPYFVNNVRVTGVSHVSLTALEAAFTTLEGADKD
                     SILEKLSVTDKNGQTVAVTDLVLDLTSNKVRVLGDFNQENAGYTLKYGNDSFTTTMSW
                     QLKDELYAYDGELGARVSQAGSVVDMTLWSPSADSVAVVLYDKDDQSKVVGKLAMTKG
                     DKGQWDLELNSQSGLGIADYRGYYYHYEITRGDQSVLVLDPYAKSLAEWNSDLADTDP
                     SYRIAKAAIVDPAEVGPSDLNYATIPNFNQREDAIIYEAHVRDFTSDPAISDELTAQF
                     GTFAAFAERLSYLKELGVTYIQLLPVMSYYFVNELKNAERMSEYASSNSNYNWGYDPQ
                     SYFAFTGMYSTDPTDPMKRIEEFKNLVNEIHKQGMGVILDVVYNHTSKTFLFEDLEPN
                     YYHFMEADGTAKSSFGGGRLGTTHYMSRRVLVDSIKYLVDEFKVDGFRFDMMGDHDAE
                     AIELAFTEAQKLNPNIIMLGEGWRTFTGDANQPVQPADQDWMSSTDTVAVFSDEIRNT
                     LKSGYPNEGQPAFITGGAKSVESVFNNIKAQPGNFLADDPGDVIQYIAAHDNLTLFDI
                     IAQSIKKDPSIAENYTEIHQRQRLGNLLVLTAQGTPFIHSGQEYGRTKQFRHPDYKEP
                     VTEDKVPNKAHLLTNADGTPFDYPYYIHDSYDSSDAVNKFDWTKATDEALYPENTRTQ
                     AFTKGLIALRKSTDAFRLVTKEEVEQKVSLISIPGQNGIAKNDVVIAYQTIASNGDRY
                     AVFVNADSKERSFVLSDIYKELLKGQVLVDGKRAGIAALTDLVGVTLTDDAVVLAPLT
                     ATVIRLPYITTVVPDTAPTAEEKPSLDFTTKERTEEKILPIVEEIRYDASLSTDQSYV
                     LQEGKTGKKVRVYQDVLIDGKVVATNLLSETVVDSETRIVVKGSMEAKKEAEKPSRST
                     TAQASGQATSGASKASLPITGDRQSDLALLGLGLAGLGLTVAAQGRTKKSEE"
     gene            complement(17838..18779)
                     /locus_tag="VCA20_000916"
     CDS             complement(17838..18779)
                     /locus_tag="VCA20_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEP1834763.1"
                     /translation="MTTLADVARLANVSKMTVSRVINHPEQVTQELRTLVTAAMEELN
                     YKPNVAAKALAQQRTLIVQAVILEKMDVVEPYYIDLLAGIADELNHRNYTLQLVTNPS
                     QITDQCDGYIVTGARKDDYPWLKGLRKPLILFGENQEGLPFVDSDNRLATMSATQFAL
                     SKGYERIVFVGIDLPEAFEKGREEGYKDAMQGRATHLYRLENRSRVAEEFVKNLDYLP
                     ENTCFICASDRLALGVIRGLQALGKSIPNDVGVIGFDGFFLDRMSRPALTTMKQPIRQ
                     MGALSVKHLMTILDGKPLARQGHYCQATLVERETTPQ"
BASE COUNT         5168 a         4413 c         3862 g         5555 t
ORIGIN      
        1 actactttga ttgatgtttg gttatgattt tcgttttctg aaaacgattt tttcttcatg
       61 cgtttggcgt gcgattagga ttgatccact tgggatttag tagccagctt atcagctttt
      121 tgcttctccc tacgttgtct gaaaaagtct tgcatgattt gggcacagtc ctcttccaag
      181 atccccgtct caacctccac acggtgattg agccgctcat ctgtcaaaat atcataaaga
      241 ctcccagcag caccaaactt ttggttggta gcaccataca ctacctgagg aattcgggca
      301 agacctattg ctccgctaca cataacacag ggctcaatgg tcacaaagag ggccgtatcc
      361 aacaaacgcc agctgccttc tgatctatta gcttcctgaa tagccataac ctcagcatgc
      421 ataatcgcct gattgagctc ttcacgagca ttgtgcccac gaccaataat ctgtccatcc
      481 ttaacgatga cacagccaat aggaatttca ttcttttcca gtgatttttc agcctcttct
      541 aaggcttgtc tcataaaata ttctttttct tctaaggtat agttcataaa attattataa
      601 cagaaaacag cctcgaaagg ctgtctctga cattttctat tacaattcgc caactaattt
      661 cacaacattt tctactgtga agccgtagtt gtcaataact gtttgggctg gagctgaggc
      721 accgaatgtg tcgataccaa ggactttacc gtcaagaccg acatacttgt accaactttg
      781 agttgcaccc atttcaattg ccaagcgacg acggatggca tttggtagga tttcttcctt
      841 gtaggcagca tcttgtgcat cgaagagttc agttgatggc acagaaacca cacgaacctt
      901 agtacctgct gcttctagtt ctttggcagc tttaacagcc aagttcactt ctgaaccaga
      961 tgcaagaagg attgtatcaa agcctgctgc ttcataaaca acataagccc cttttgcaac
     1021 cttgtcaaag tctgttcctt cttcaactgt caagttttga cgagtgagga caagggctga
     1081 tggtgtagac ttgctagtca atgacaagta ccaagctgcc tgtgtttcac gcgcatctgc
     1141 tgggcggaag acattgaggt ttggcatagc acgaagtcct gccaagtgct caatcggctc
     1201 gtgagttgga ccatcttcac cgactgcgat tgagtcatga gtgaagacat aagtcacagg
     1261 aagtccttga agagctgaca aacgaactgc tgcttttaca tagtcagaga agacgaagaa
     1321 ggtaccacca tagacacgaa gtccgccgtg ggctgccata ccattcaaga tagttcccat
     1381 ggcaaattca cgcacaccga actggatatt gcggttgagt ggggttgctg catcctgcaa
     1441 accatcttcc ttgatgtagg tcatatttga gtgagccaag tcagctgaac cacctaagaa
     1501 agttgacaat actttagccg ctgcgttgag cgcatcttgt gatgagttac gagttgcttg
     1561 tgagaagcca ttctcataaa ctgggaagtc ttctggcttg atttcgacta catcacgacc
     1621 ttccaagata gctgttactt cagctgctaa ttctggatgt gctactttgt agtcttcaac
     1681 taatttcacc caagcatcgt aggctgctgc accgcgatca gctacattag ctttgaagtc
     1741 cgcatagact tcagctggaa tttcaaatgg accatagttc caatcaagcg ccttacgagt
     1801 tgcttctgtt tcttctgcac caagtggagc accgtgaacg gcatttgtac cttgtttgtt
     1861 tggcgaacca tgaccgataa cagtcttgat ttcgataaga gatggcttac cagctacttt
     1921 tgccttttca atagcagcaa agatggcatc tacatctgta ccgtctgtta cgaggtctgt
     1981 ataccagcca taagcattgt aacgggcacg tacgtcttct gtgaaggagt cttttgtttc
     2041 gccatccaag ttgatgtcat ttgaatcgta aagaacgatg agtttttcta atttttgaag
     2101 acctgcgtaa gaagccgctt ctgctgaaac accctccatc aagtcgccat caccacagat
     2161 tacgtaagta tagtggtcaa agattgggaa accatcgcgg ttgtacttag ctgcaaggaa
     2221 acgctctgct tgcgcaaaac ccgtcgcagt tgagatacct tgtccaagag gccctgttgt
     2281 tgcatctaca cctgctgtgt gaccaaattc tgggtgacct ggtgtttttg aaccccattg
     2341 acggaagttc ttgatttcat ccatagtgac atcttcaaat cctgacaaat gaagaagtcc
     2401 gtagaggagc attgacccat gacctgctga taagataaag cggtcacggt tcacccagtt
     2461 gggctgagct gggttgatac gaagttcttt agtaaaaagg ctataagcca tcggtgccgc
     2521 acccataaca acacctgggt gacctgattt tgatttttcg atggcatcaa tcccaaggaa
     2581 acgaatcgca ttgactgaaa gatttgacat gtttttctcc ttttttaaag tcattaattt
     2641 attataccat aaagcgtttt ctatgtctgt ttgaaatgaa gtgagaaata aattgtccgt
     2701 aaaaaagagg caggactttc gtcccgccct tggagtgaac agtagtaatt cgcacatttc
     2761 atactttatc agagtttata gtgcctctcc tcgtctctat ggggcaagag agttctaaat
     2821 cttataacaa agccttaaat tgaatatttg agtcttctaa gaagcaatcg tcaaagccac
     2881 gtgggtgatg gtattcaatg cggtctttgt caagtggata ggtgtacttg ccaccaactt
     2941 cccagatgaa tggatggaaa tcatattgca agcgttcttt acgcattttc caaagttgca
     3001 agatttcatc tgttgatgcc atgaagttag accagatgtc atagtgaaca gggataatga
     3061 ccttagcacg caagttttct gccatacgga gaaggtcaat tgaggtcatc ttgtcctgga
     3121 taccgattgg gttgtcaccg tagttgttga tagcaacgtc aatgttgaag tcacgaccgt
     3181 gttttgcaaa gtagtttgag aagtgggagt ctgcaccatg gtagatggtt ccgccaggtg
     3241 tttcaaagac atagttgact gccttgcgag ccatgagttc atctgtcaca ggaagtcctg
     3301 ctaatttacc accattttcg tcataacctt cgattggcaa ggtcaccaag caagtacggt
     3361 cgaaggactc aactgcgtgg accttgatgt ctttgaactc aaagctatcg cctggtgcga
     3421 tacggatgat acggtcagct ggaacacccc atttttccca gatgtcgcca ctttcaactg
     3481 gaccgacaaa tttgacgtgg tccaactttg gattgttgat gatggcagcg gctgtgctga
     3541 tgtccatgtg gtcactgtgg acgtgcgata ctaggtagta gtccaattca ttgatggcaa
     3601 atgggtcgat aaccattggc tgcacacgca agtttggttg caatttgcgt acaccggcca
     3661 tgttagccat ctggtgtccc caaaccatat ctttcacttt tttagtggat tttccacgtg
     3721 atgaccagag gtccatgacc acgttagctc cacctggtgt tttaatccag acaccacagt
     3781 ttccaagcca ccacatagca aagttgcctt ctggcactac ttcttcttca atttcttcat
     3841 tgagccaagt tccccattct gggaaggttg aaagaatcca ggattctctt gtaatatctt
     3901 gaactttagc cattttccat tcctcctata aggtacgttt ctatgcttct agtatatgct
     3961 ccaaaggtaa caaaaaaaag accaaataaa acacaggctt gtgttcaaac ttggacttta
     4021 ccctaataca gatctgtttc aatatcttga ctggataaat tggttaataa ttcattggta
     4081 atcagctttt gtagccgttg tttgagttct tgagcggtcc ttccgtctgc gatatatccc
     4141 gatagaagct tgtctagttc tactgcaaaa acttgttttt catctgccaa actacgatat
     4201 tttgcaaaat gagtcatgtt tagcacatca tcaaaatcta aaatggggtg aacctgcacc
     4261 aaagggaaat ctgtttccaa cccatcgctg gttgtaatca agaaatcaac ctctagtaag
     4321 tcttcgacac tcttgaattg ttcagtggta aagacggcgc caatcttctt atctggcaag
     4381 tgatgctggc attgtttata aagtaagcgt tggactgaca ccccttcatc acagattaaa
     4441 tagacaatct gtgtctgtgc cttgcgcgtc ccactgcgtc gaatagcccc tcctagatga
     4501 atcgtcaaat aggcaatatc tccatcggat aactcaatct gccactcctc ctccaaaatc
     4561 tttccagaaa cccgtgtcat agcaaacagg gtgccgtatt tatcttggat ttgttggacc
     4621 attggatttt tagataggat gccgtaggtt tttctgaaca acagagcctt gcaatgtgcc
     4681 aagagatgtt tggctaattc gtcaggattt tccaattcaa atggtgaatg tatctcgaaa
     4741 tgatgaataa atcgctctac cacacgcttc aagtcaatga aatcttggct gaatacatga
     4801 acatcgctgt cctttcgata ggatagaagt aaaatggcaa tcagggagat ttctatttca
     4861 tccaatttca aatcaaaggt ttgcaacaag gcatctttca attcctgagc aactcggtat
     4921 tcgatacgct ctcgaacttg ttcaaattct ttgagaatgg ctcgttcttc caagtcatcc
     4981 agctccatat tgcgatagct aaggagcaag aaaggcaata ctttgagcat aaactcgatt
     5041 tcgtagcgat tgattttctt gcccagtttc ttttctagct gtgatacttg tccctgtaaa
     5101 aattgtgcca tttcttctga gaagagtaac tgacatccta ccaactcaaa taacttatct
     5161 tctagaacct gcaaaaaacc tttattggca tccaagaaga tagtatagag aagtgagtgg
     5221 acatattgga ttttatttaa agggtgacaa accagactat agccctgact cttagacaca
     5281 tagagggtca tcttgtattg ctcttgagcc aactgggttc gaatttcatt caaatcattt
     5341 aagaccgtat tacgagagac ttctgtcaac tccattagac gttcaatggt cactctttct
     5401 ggtgtgatac agatatatac caacatcagc tgaatgcgct caggcatgct catgacgtag
     5461 gtgtacgaat caatggaatc gagaagatct cgacaggcta gtttttgcgc ttcggtaagt
     5521 cgaataccga ggcgaggtaa gctttcaata ctctctacag ttgttggtag ggcatcattg
     5581 attttttcta gatggtaata gatttttcga cgtgattgtt gcaagccctt ggaaatagcc
     5641 atgattgtct caggttcctg tagaccaatc aggtacctca gtagagcgca actactctta
     5701 tctaataaca agattctcct cctctttccc ccgtcccgtt actatcttaa cataattatc
     5761 tgggatattc taataattat taaaattcat ctgattatta cttgttttac attgcataaa
     5821 tatttgtagt tagaattaat agatgctttt aataatttct acccgggtaa tcagttctcc
     5881 gtgcatgtta tctatccgaa gggcaacatt tttcaaatat acacaggcac caatagcaat
     5941 gtttttctct tcttcattga gttttcgtgt cacaaaatct ctcaaagtct cgctaccttc
     6001 acttggaatt tttactcgtg cgatgatatt agccactttt actttctggc tggcgcgcat
     6061 gataatatta ttggaatcat caaactgagt gaagaggtat tttctaactg caaataggat
     6121 ggcgaccgaa ataattccaa tcaagtaatc actactagaa ccatgctcta caatcagttg
     6181 gcgagcaatg gcaaccagga gtacctcaat cacggtgcta ggtgactgtt tgcaaagcat
     6241 tttgataaat tcgactccga ccgccaaggt cattgcacga ctcagaaaat tttgaataaa
     6301 cgtatccgct tgaataccac cagctaatga gctgagtagg tctgcaaaaa gaagtgttga
     6361 caaggctaaa agtgctaaac caagtatggc agataaaatc aattctaaat agtaggctgt
     6421 ctctgtcaga ctatcctgta aaaatttcct catctttctc ctcatcaaaa gggaaaaagc
     6481 agttctcctt tttggggagt ctgctacttt tccctactgt tttttctcac acaagaactt
     6541 aatgtgcatc acccttttgt ccatagtagg cattgggacc atgtttacgg aggtagtgct
     6601 tgtccatgat atagcttggc gctggctcca cccgtggatt gatttgttcg gtgtagcggt
     6661 tcatccgggc cacttcctca aggacaacac tatggtaaac tgcctgagca ggatccttgc
     6721 cccaggtaaa tggtccatgg ttacggacaa caattcctgg tacagtaact gggtcgatac
     6781 cacggcgttc aaattcctcg atgatgactg aacctgtttc tttttcatag gccgagttta
     6841 cttcctcagc tgtcaaagaa cgggcacatg gtaccggacc atagaaatag tccgcatgag
     6901 ttgtcccata aaatggaatg tcacgacctg cctgagccca acccactgct tctgtcgaat
     6961 gcgtatgcac gacagcatgt acgtttggcc aagccttgta gagggcaacg tgggtcgcca
     7021 aatcagacga aggattcagg tctccttcca ctacatttcc gtctaagtcc gtaacgacca
     7081 tattttctgg tgacaatttt tcatagtcca caccagaggg cttgatgacg atacgaccaa
     7141 gttcacggca gacttctgat acgttgcccc aggtaaactt gacgagccca tgagcaggta
     7201 gggcgatatt ggcttcatag acccgctgac gcatttcctg taaatctttt ggcattagtt
     7261 taaccccgct ttctcaatca atggatagag gaaatcttgt gcttctttga tagctgctcg
     7321 tgtttcctca actgtttggc agttttcaga ccacatctca atcaagaatg gaccttggta
     7381 gttggtcttt ttcaagactg caaacatatt ttcccagtcc acacagccct gaccgaatgg
     7441 gacatcacgg aattgacctt tggagttttc agttaccgca taggtatcct tgaggtgaag
     7501 ggctgcgata gaacggtgtc ccagataaaa ttcgctgtaa atgtcattgt gccaagcaga
     7561 cacgtttcca gtatctggat agacaaagag ataaggcgag tcgatttctt tttcaacggc
     7621 taggtatttt tcaatggagt tgataaatgg atcatccata atctcaatgg ccaacatgac
     7681 ctgagcctgt tctgcccagt cgcaagcctt acggagattc ttgatgaaac gctctctggt
     7741 ttcaggtgat ttttcctcgt agtaaacatc gtaaccagcc aactggatga cacgaacgcc
     7801 caaatcctga gccagttcga tacactgttt catggtttct aaggacttag cttcaagagc
     7861 agggtcattg gagccaagcg gataacgacg gtgaccagag aaacagatag tcggaatgcg
     7921 gacacctgtt tcatagatag ctttaaccaa gtctaagcgt tcttccttgg tccattccaa
     7981 acgagccaag cgtgcatcgc tctcatccac cgacatctcc acaaagtcga aacctaattc
     8041 tttggcaaac tccaagcgtt ccttccatgt gaaatgcttg ggcgttgcct tttcatatat
     8101 tccgattggt cgtgccatag gtcttacccc cagatacgac ggatttcgtc tttaaattca
     8161 cgcgccgcag ctgctggatc tgctgcttct gtgataccgc gacctgcgat aaaggtgaag
     8221 acatctacac ccgcaaagag tttgagggta tccacatcaa gaccacctgt tacagaaaca
     8281 cggaagccca tgtcaaccaa ttttttcact ttattgaggt ctttttcacc ccaggtttcg
     8341 ccagcaagga gggcgtcacg cgattggtgg taaatcgctt gggaaatacc tgcgtccaac
     8401 caaagctgag cttgttcaaa cgtccaatca ccgtaaagtt cgatttggat ttcacccttg
     8461 tcgccacgca cttccttgat agccttcaaa gcagctgcca tagttggaat agttgctgaa
     8521 caaatacaag tcatccagtc cgcaccacga acagcattgt ttttagccac agtaccaccc
     8581 gcatccgcac acttggtatc tgcaacgata attttgtctg ggaagaggct acgaagaact
     8641 tctaccaatt cgctaccaac ttgaagcaaa caaacggtac ctgcctcgat aacatccact
     8701 tcatgaccaa cggagacagc tgctttaatc gctccctgca agtctgaatg gtcaagggca
     8761 acttgtaaat ttggaatatg tttcatgttt tttctcctaa ctttctaaat ctaaaccttc
     8821 taggtaagga ctgtttttgg actcttccac cattgccaag acttcctcag gagtttgaca
     8881 agcaatcaaa cgctcgattg aattttccaa ttcgaagagg gcaacgattt gtggaatagc
     8941 tacggatgta tgaatctttg gatcggtagc tgcaagagtg agcagaacag acacttcttt
     9001 cccatctgag aagacgactg gttttgtcaa ggtaatcaaa gcaaaggaat ccttcaagac
     9061 gccgacacct gcttgggcgt gaggcatagc cataccaggc atgagcacat agtagggacc
     9121 atattcttcg gtcgaagcaa taatggcatc atagtattct tctgtcgcag caccgctgtc
     9181 aatcaaggga tcaacagcct tgtgaacagc ctcctgccag gtttccgctg tcaatcccaa
     9241 gcgaatggaa ttattttctg taaatgattt ttgtaaattc atagtttttc ccttctacac
     9301 gctatatgaa aggggaggga aactccctac ccctaacagc tatttatagc actgcttgta
     9361 attttgtttt gatttcgttg tcgtccatga ggttgtcaag accaaccaaa tgacctttcg
     9421 tgcgaccttc caactcagaa atcaagtggt tagaggctac gacaatatca tatcctgccg
     9481 ctaaaccttt tgcttcaccg actgagcaag aagctgattg aaaatctgtt acgccgagtt
     9541 gacgaagggc gttttctacc ttcatcttga tgaccataga tgagcccatg ccgtttccgc
     9601 aggctgttaa aactttaacc ataatttcac tcctatttta tttttaatac attatgttct
     9661 gattttacag accaaggata ctattctgct tgtgcctcgc cacgatagta cgcttcctta
     9721 tcttttgctt tcgcaaattg aagttgtgga atagccaata agaagaggca gacaagaaca
     9781 taaccggcaa taccgaggta cttaaatgca aatccaaatg gtaaccatgg cacttcaaag
     9841 tcaatgtttc cgtggtaacc accgctcaat tcaagcaaac caacgcagac tgcaccaaga
     9901 gcaacttgaa ggacacctga gatgaaggaa aggatggctg ccgctttcca accaccacgt
     9961 ttgtcagcgt acactgcgat agctgcgtta tcgaagaata caggtacgaa accagtgatg
    10021 atgaggattg gactcttgaa gacaatcaag agaacgattg taatcaactg accgattaaa
    10081 ccgaaggcaa aacctgaaag aactgcgtta gatgaaccaa agccgtaaga agctgcaacg
    10141 tccaccgctg ggaatgaacc aggaagcaat ttgcttgaga taccttggaa ggcatttgtc
    10201 aattctgcta cgaacatgcg gacaccttgc atcaaaatga aaagatatac tgagaaagtg
    10261 aaggctgttt gaatcacata cataaagaaa tcttgtttag ctgggttgta tggagcacct
    10321 gaagtgataa cttctggatt tgccatgatt tctggaccca agatgaacaa aatcacaccg
    10381 aagaacacaa gcatcaatgt cgctgaagca acgactgtat cgtggaagat attgaggaag
    10441 ctaggtagct tcaaattatc caagttttct tcttttttac caagttttgg tgcaatctta
    10501 tcgacaaacc agatcgcgaa ttgttgttgg tgaccgatgg caaatccacc gccacctgtc
    10561 aatcgttggg ttggctcaac tgtcatgttt gagctgactg cccagtagat accacagatg
    10621 ataccaatcg ctgctgtacc gaattggtta cgaagagccg ggataaggaa gagtaccatc
    10681 aacgagatgg ttgccgcttg ttgtaccatg atgtgtccag tgatgaacag agtacgtact
    10741 ttggtaacct tacgtagtgc caccaagaga atgttgacac cgaaaccaat caagagtgct
    10801 gtagtggctg ctgatacgaa atcagggaac tcctcagcaa ttttcccatt tgctgctgca
    10861 agaccgaagt aagggtcaat aactgccgcg tcaatctgga acttgtggtt caaggccgcc
    10921 aaaatcggac ggaaggttgt taccaaacca ccagaagcta cgttcaaaat catatagccg
    10981 actgtcgctt tgataaaccc tgcaaaaaca tcatgaggct ttttctttaa gagggcatag
    11041 cccaccaata ccaaaagacc tacgaagaag gctggattct gcaggatatt ctgtgagaac
    11101 cagtttaatg gggtttgaag gaaatccatt tttcattctc cttttttgaa atgattttta
    11161 tggctttatt ataagttatg taagcggaaa caaaaagacc aaataaagca caggcttgtg
    11221 ttcactattg gactatttcc gaactttaat ggctgattta atcagaattc ctccctatag
    11281 ttcaccagcc catttattct aataatcaat aaatagacgt tattttacct aaaaatttaa
    11341 aagaacctca aaaaaacatg aaagaagagg gaagtgatac tctcaactct tgtatttata
    11401 agacattcac aaggttttga gtagtgctcc tctggagagc caaatattat tactcttctg
    11461 attttttagt tcttccttgt gcagcaactg tcaaaccaag tccagcaagt ccgagaccta
    11521 gaagtgccaa atcgctttgt cggtcacccg taatcggcag actcgccttg cttgcaccac
    11581 ttgtcgcttg tccagatgct tgagcagtag tagaacgact cggtttttca gcctctttct
    11641 tagcctccat actgcctttc acaacaatac gtgtttcgct atcaacaact gtttctgata
    11701 acaaattagt tgcaactacc ttaccatcaa tcaagacatc ctgataaacc cgaactttct
    11761 taccagtttt gccttcttga aggacatagg attgatcggt agagagactt gcatcatagc
    11821 gaatttcctc tacgatagga agaatttttt cctctgtacg ttcctttgta gtaaagtcta
    11881 gacttggttt ttcttcagca gtaggcgctg tatctggaac taccgtagtg atgtaaggca
    11941 aacgaatcac cgttgcagtc aaaggcgcaa gcacaactgc atcatctgtc aatgtcacac
    12001 ctaccaaatc agtcagcgct gctattccag cccgcttacc atcgaccagc acttgaccct
    12061 taagcaattc tttgtaaata tcagaaagaa caaagctgcg ctccttgctg tcggcgttaa
    12121 caaagacagc gtagcggtcc ccattgcttg caatggtttg ataggcaata accacatcat
    12181 ttttggcaat gccattttga cctggaattg aaatcaagct aaccttctgt tcaacttctt
    12241 cttttgttac caaacggaag gcatctgtag atttgcgcaa ggcaatcaat ccttttgtaa
    12301 aggcctgagt acgggtattc tctggataaa gcgcctcatc tgtcgctttg gtccagtcaa
    12361 acttgttgac cgcatcagat gaatcataag aatcatggat gtagtatgga tagtcaaatg
    12421 gtgtaccatc agcatttgtc aacaaatgcg ctttgttcgg taccttgtcc tctgtaactg
    12481 gttccttata atcaggatgg cggaactgtt tggtccgtcc atactcctga ccagaatgaa
    12541 taaacggtgt cccttgagca gtcaagacta gcaaattacc caaacgttga cgttggtgaa
    12601 tttcagtgta attttcagca attgatggat ccttcttgat ggactgagcg ataatatcaa
    12661 agagggtcaa gttatcgtga gcagcaatat attggatcac atcacctgga tcatctgcca
    12721 agaaattacc tggttgtgcc ttgatgttat taaagacaga ctcaacactc ttagcaccac
    12781 ctgtgataaa ggctggctga ccttcgtttg gataaccaga tttaagtgta ttgcgaatct
    12841 catctgagaa taccgcaacc gtatctgttg aactcatcca gtcttggtcc gccggctgaa
    12901 caggctgatt ggcatcgcct gtaaaggttc tccatccttc accaagcata atgatgtttg
    12961 gattgagttt ttgagcctcc gtaaaggcca gctcaatcgc ttcggcatca tggtccccca
    13021 tcatatcgaa gcggaaacca tctaccttaa actcgtccac aagatacttg atagaatcaa
    13081 cgaggacacg gcggctcatg tagtgagtag ttcccagacg accaccacca aagcttgatt
    13141 tagccgtacc atcagcctcc atgaagtgat aatagtttgg ctctaaatct tcaaataaga
    13201 aggtcttaga agtgtggtta tagaccacat ccaaaatcac acccatgcct tgtttgtgga
    13261 tttcattgac caggtttttg aattcttcga tgcgtttcat tggatcggtc ggatctgtag
    13321 aatacatacc agtaaaagca aagtagctct gtggatcata gccccagttg tagttgctgt
    13381 tgcttgatgc gtactcgctc atccgctctg catttttcaa ttcattgaca aaatagtagc
    13441 tcataactgg tagcaactga atataggtca cacccagttc ttttaggtag ctgagacgtt
    13501 ctgcaaaggc cgcaaaggta ccgaactgag ctgtcaattc atcggaaata gcaggatcgg
    13561 atgtgaagtc acggacgtgt gcttcataga taatggcatc ttcacgctga ttgaagttag
    13621 gaattgtggc atagttcaaa tcacttggac caacttctgc tgggtccaca atcgcagctt
    13681 ttgcaatacg ataagatgga tctgtatctg ccaaatcact attccactcc gctaaagatt
    13741 tagcatatgg gtctaaaaca agaacagatt gatccccacg agtgatttca taatggtagt
    13801 agtaaccacg atagtcagca ataccaagac ctgattgact gtttaattca agatcccatt
    13861 gtcctttatc gcccttggtc atggctaatt taccgactac cttagattgg tcatccttat
    13921 cgtaaagaac gactgctaca ctgtctgcac ttggagacca gagagtcata tcaacaacac
    13981 tgcctgcttg actcacacgc gcaccaagct ccccatcata agcataaagc tcatccttca
    14041 actgccagct catagttgtt gtgaagctat cattgccata tttgagggta tagcccgcat
    14101 tttcttggtt gaaatccccg aggactcgca ccttgttgct ggtaaggtcc aagacaaggt
    14161 ccgtcactgc aactgtctga ccattcttat ctgttacaga caatttctcc aagatgctat
    14221 ccttatccgc accttccagc gttgtaaagg ctgcttctaa ggctgttagg ctaacgtgag
    14281 aaacacctgt cacacggaca ttatttacaa aataagggtt ggtgtaaata gtcgcatcat
    14341 catccttgag gaagatttgt gtctgatttt tcaaatcttt aaagctataa tctttctgct
    14401 gaatcttcac atcatctcct gacttgcttt catccaatag caggaagcca atggaacttg
    14461 gtaaatctgt caatttgaca tcaatatagg caccgtactt gccttggttt tcaaaatcaa
    14521 taccatttgg ccagtcaccc aaggtaaggt cagtcacatc accccaaatc cagagacctt
    14581 tttggtcata attgccatcg gtacggaagt aattgatacg gatgtagcct tctttaagcg
    14641 gttgatagag gctgagttgg tagttttcat caaaccaagc ttcattcatc ttggtgctaa
    14701 tccgttcaat agtcttgtca cctgtgatgt tatcaccaga agtgttatta atcaacagac
    14761 taatcttgct ggctgtttca tctttcatct tgatatcaag ataatagcca taatcatcct
    14821 gcttagcagt actgaaatta gtcgctcctg ttggccatgc accaagatct gacgatggtt
    14881 tttcgacatc atcccatgtc cataaaccta ggctagataa attatcagat ggtagagttt
    14941 taaagtgaac acggaagtaa cctgcttcaa ttggttcttc agttgctcct tgcacacttg
    15001 cattgacgat gtcacctgct tctgtctgaa ggagcatggt gccaccttga gcgatagttg
    15061 gaagttcgac ttgaacaacc catttaccgt ctttctcgat agcttgataa gattttcctg
    15121 tgtaggcatc tttgaccact acatccttac cagatacttc aagcgccatt tcctggttct
    15181 cggtattggt tgagaagaca agataggcag cttgatcttg gtaactgcgt ttgctgataa
    15241 gccaaccttg gctatcattt ccagcaagtg ttgttgtatc accacgtgag agcaatgttg
    15301 aattggcatt acggaaggcc aacaattttt ggtaatggtc ctctatgtca cttgtttcta
    15361 ccttactcca atcgaaatcg taccggttat cataaactgg ccagttgttt tgtccagatt
    15421 gccccacttc ttcaccgtag taaatgactg gctgaccttt agcagtaatc aaaagactgg
    15481 cagccaattt cagtttgtcc agatttcctt ctttttctgc gccaccgaga ctgtagagga
    15541 aaccatcttc gtcatgactg cccaagaatt gacccagaga agcagcacta gtcagaacct
    15601 tgctacgttc ttccagctct tttccagctg cttttagttg accattgacc agatattttg
    15661 caatatcctt gaagccaaag tccaacaagc tgtccatagt accgataccc aagtcaccct
    15721 tggtatcttt atagttagct ccccaagttt ccccaatcaa gtggaagtca ggatctctat
    15781 ccaccaattc atttttgaaa tgttgccatg tggtatcatc aacgtgtttg actgtatcga
    15841 cacggaaagc atagatagag ttgcccttgg cagttgttga gcgttccagc caagatgcct
    15901 gccaagcaac caactgattg cgaacagctg cttcttctgt cttgaagtct ggtaatccag
    15961 ataatgaacc taattggtca tctccttgct tgtcttcttc cttagtccgt accatacctg
    16021 caaaggtttc ttctgtacca tagccagcat ggttaagcac cacgtcaaca acaatgttta
    16081 tacctttttc cgccgcagca tcaatcaatt cgtggaaatc agctaaactt cccaaatgcg
    16141 gatttagttt ctcaaaattg gatgcccaat aaccatggta agcatagtac tctccatctt
    16201 ttacgctacc aacattctgt ggaatatttt ccacaatcgg agtcaaccag atagagtcca
    16261 catccaattc tttcagatag tccagcttgg ctgtcactcc tttgaagtct ccgccttggt
    16321 aaagaccgcg tgggttggag gctgttcctt ccactagctt gtcattgctg gtgtctccat
    16381 tgtagaaacg gtctgtcatc atgaagtaga tgacttgctc gtcccagtct ttttcaccat
    16441 ctgccttgat acgctctgta atggtcacac gattgagctc agtagagatt ggcacaagac
    16501 ctccgccgat ggcacttgta tcgacttcca ttttgacaat ctcagcattt tcaggattgg
    16561 tgacagttac agataccaag ccgctttcat tgtagttgaa tggtgctttc acacttgcct
    16621 caagggtcac attttttgta ccaggagtcg ctttacttgt atcgagttct actttttctt
    16681 cgataaagta tggattgata tagactttgt cgtcgtcatt gcgaaggaaa atttggctat
    16741 gccgtttacg atctgcaaag gctaaatcaa ttgttttatt gcctgccaaa tctggattat
    16801 tctgattgat tagcagaaat ccaagcttgg aatctaaacc gttcgagagg ggaatgtcaa
    16861 cataagctcc gtatttgcca agtttaaaat caacggcacc tgttggccag ccatcactcg
    16921 cttcagtcgt atcgccccaa gtccaaacgc cccaaccttc ataattattg tcttcacggg
    16981 tgtagttgat acggaggata ttttcatctt taagcggctc atagctagac acagtataat
    17041 cggtagcaat ccaagcctca ttttgttccg gaacaagaag ctccactttt tggtccgatt
    17101 ctgttatttt ttccccatct ttaaggacca gatagccgat cgttcctggt gtctcagatt
    17161 ttttaatatc aacgtaatgg ccgtagtcat ctacctgact acttgaaaaa ttcgctgtat
    17221 ccgttggcca ttgattacca tcggacggct ctgccactcc tccccaagtc caaagtcctt
    17281 gactttctgg tgcgatttca tctacatttt caaagtgcag acgaatgctg ccttcttcga
    17341 taggagcttc ctctgaagca gttgaatcgg ctgtttctgt taccgctgtc gcttcagcta
    17401 ccatagaatc tgtagcttct atcgcagttg cggtcgcttc taccgcgccg ccatcctgtt
    17461 ccgaaagagt cgttgaacta gttgacgcag ggaggacaac ttcctctgcc tgcactagtt
    17521 gacctgctcc acttaaaaac agagcagccg caatcccaac agaggctaca cctacattta
    17581 gtttacgaat gccaaaaatc tgtttcttct ctagcatccc tgttcgtttc atattctgaa
    17641 cggatttctt tttcatagac tcctccatat aaataagttt gaattgtaca ctaccagtat
    17701 atacaattcc aaggagtctt tgcaaacgtt ttctcaaatg ttagcggtaa caagttttgg
    17761 actactatct aactaaaata gaataaatag ctcccccttc tattgctcct agaccgtcca
    17821 ctgaccaaaa aaagagacta ctgaggtgta gtctcccttt caactaaagt tgcctggcag
    17881 taatgcccct gtctagccag aggcttgcca tccaatatcg tcattaaatg tttgacgcta
    17941 agagccccca tttgacgaat cggctgcttc atcgtcgtca aagctggtct ggacatccta
    18001 tctaaaaaga agccgtcaaa accaatcacc ccaacgtcat tcggaataga ctttcctaga
    18061 gcttgtaaac ctcttataac cccaagagcg agacgatccg atgcgcagat aaagcaggtg
    18121 ttttctggta aataatcaag atttttcaca aattcttcag caacacgaga acgattttcc
    18181 aatcgataga gatgagttgc tcggccctgc atcgcatctt tgtagccttc ttctctccct
    18241 ttttcaaagg cttctggcaa gtcaatccct acaaaaacaa tcctctcata accttttgat
    18301 aaagcaaact gggttgcgga catagtcgcc aaacgattgt ctgaatctac aaaaggaagg
    18361 ccttcttgat tctccccaaa taaaatcaat ggcttccgca gccctttcaa ccaagggtag
    18421 tcatccttgc gagcgccagt cacaatgtaa ccatcacatt gatcagtaat ctgacttgga
    18481 ttggtcacca actgcaaggt ataatttcga tgattgagct catcagcaat tcctgcaaga
    18541 aggtcgatat agtacggctc taccacatcc atcttttcga gaatgacagc ctgaacaatc
    18601 agtgtccgtt gctgggccaa ggccttggcc gccacatttg gcttatagtt taattcttcc
    18661 atcgcagctg ttacgagcgt ccgcagctct tgtgtaactt gttctggatg gttgatgaca
    18721 cgcgaaacag tcatctttga tacattggct aacctagcca catcggctaa tgtcgtcata
    18781 gaactcctac ctaattctat ttctgttgct tatcttttag caagcaaaca gtcaaaattg
    18841 tgcaaacgtt ttctttaggt tattatatac tatttattac taaaaagtaa agacctttcg
    18901 aatacattta tcgtcattta gttttcctcc tattacctcc ttagctcctt acctaacttc
    18961 atctatgcct gcggctcatc gcctcgttct aaaagtac
//
LOCUS       DATUCG010000034        18411 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.035, whole genome shotgun sequence.
ACCESSION   DATUCG010000034 DATUCG010000000
VERSION     DATUCG010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18411
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.035"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            <1..219
                     /locus_tag="VCA20_000917"
     CDS             <1..219
                     /locus_tag="VCA20_000917"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834764.1"
                     /translation="PYNSGAAIAEFLFSIYGKDMKQDEFRKIIDYIKERSKWKDANFQ
                     TWRLVLKRALSSPATTLEEFIINEEGIE"
     gene            216..1790
                     /locus_tag="VCA20_000918"
     CDS             216..1790
                     /locus_tag="VCA20_000918"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012235.1,HMM:NF012869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1834765.1"
                     /translation="MNKKRTISFFRQLIKSIGITQVCIYVGIIVILFLLIGIQPFLIS
                     ELFSFTKLTIDYTYLFLLLLSFLSVSFVAFPKNYMLQKVRKVSKSIVFKDISDKSYSK
                     FVDMNLGEVQNLASEISYSARSLQYESISFILNVILIISLYAFSLITYDIFIGGVYIL
                     FCSLYLWLSIVFSKDNVRLIQDALDSTSETNSFFVDYFQNVEVIISTFRQFFETKKYD
                     TILNKEQKTYFNLQKRIDSHQLLLQLILSVMTVVMIVLVLNKSSDGSMAYILVLVYSS
                     IHMNGFGKQFLSVLESLDRLDLALDKIDFGRLNKVSISHENTDTDSLISLKNVQFSHG
                     DKKIFDNLTFSIRRGKFILIRGKNGTGKSTLLKLVKGMITPDSGSVTHYLSSEQTGYL
                     SQKMTLFDRTLKENMIYPLDKVDDALLMNLIAELKMTTLIQDVHGWSERKPGDFGDKL
                     SGGEKQKILIARGIINQYEVLLLDEIDSALDLEMQTQLMTILKKYYQNKTVVMVSHRK
                     IEESFFDDIIDIETIN"
     gene            2179..3018
                     /locus_tag="VCA20_000919"
     CDS             2179..3018
                     /locus_tag="VCA20_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEP1834766.1"
                     /translation="MIKLIATDMDGTFLDGQGSFDRERFSRVLDQLDQKKIPFVIASG
                     NGIGRLLQLCQGFENRLIFVADNGAHIYHQGKTLIRRAIQQVEVEAILHFFKGRWADV
                     CLMLSNDGNIYMQAGAGMPFAGTDLPIEPAQMAAFQNRVTYLDNLSAYPNFEPIYKVG
                     LWVPEARVESITEEFNQAFQGRLTAVTSGYGSIDILPEGLHKAWGLEQVLTGLDIEPE
                     QVMAFGDSDNDIELLSYVGYSYAMENATDKVKAVAKYRAPSHLEAGVMQVLENWLSET
                     KGE"
     gene            complement(3096..3878)
                     /locus_tag="VCA20_000920"
     CDS             complement(3096..3878)
                     /locus_tag="VCA20_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEP1834767.1"
                     /translation="MKKYISLYLYNIKVYMMAQMSFRVDFFIGLFSSLIEQIVYLIFL
                     NILFGNIKEIAGFNYGQMLFIYGIATVGRSIHLIFFDNLWMFGSRYIRQGEFERLLLM
                     PVNPLFQLICERIQPQGIGTTLIGSLALAQASKELGMEWSLGKLALLIFIAICIGLLY
                     AAIQLGPTALAFWIVESFPLTMGIFALNQMAQYPLNIYPKIIQILLIFVFPYAFTAYF
                     PALYFLDLSMWGLALPLVVLVLFTINYKLFRYGMTQFTSVGN"
     gene            complement(3880..4659)
                     /locus_tag="VCA20_000921"
     CDS             complement(3880..4659)
                     /locus_tag="VCA20_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEP1834768.1"
                     /translation="MRKYLYMTRLTMMNAMQYKAFFLATFVSLAVKILVALYVWKTIF
                     FTQSEVNGFTLQTFTTYIIFANLLASLNSFSLGEDLSYSIIKGSIAGEFLRPYSFILA
                     LFFKDLGNKLLELIKFAIVFIGIILVHQDFYLPDGKTILLFLVSSILGMFIVQLLDMA
                     FGFLAFFTVNAWGVMLLRMGLFNLASGALLPLSFYPQAVENFLKLLPYNYAVNVPVSI
                     LLGQESDLTALGLQVIWIPILACFIALLWSQAKRRIVIFGG"
     gene            complement(4652..5635)
                     /locus_tag="VCA20_000922"
     CDS             complement(4652..5635)
                     /locus_tag="VCA20_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEP1834769.1"
                     /translation="MIQAEHLSKTYSIIDKEVGLKGSIKAFFKPKKKAIPAVQDISLQ
                     VGKGEIIGYIGSNGSGKSTTIKMLTGVLYPDQGSVRINGLNPQENRKAVNKQIGVLFG
                     QKSHLDWNLPVQESFILHAKIYDVPDKVFKERLAVLIDLLDLADIMKQPIRNLSLGQR
                     VRCEFAAIFIHQPAVVFLDEPTIGLDASVKETIRSFIRYMNQEYQTTFLITSHDMKDI
                     ESLCERIFIIDKGKKVYDGSLTILKERFSTVKTILFSTEKPIEKDLQLEGWEFVRKDD
                     FHFEIHYQSKVWTSAQVIEQVFNHYAIEDVTMKELEIETMVRQIYEEGIHA"
     gene            complement(5720..6253)
                     /locus_tag="VCA20_000923"
     CDS             complement(5720..6253)
                     /locus_tag="VCA20_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEP1834770.1"
                     /translation="MGTDSRKERTRQAILEAMVTCLESQGFNDITTTHLAQTAGISRS
                     SFYTHYKDKYELIDSYQQGLFHKLEAIFDRYDGNRQSSFLEIFELLYREKLLSALLTH
                     NGTQEIQNFLINKVRLLVANDLAERLGKEDLSQLEKEYSSIYFSHAFFGIMQVWIKNG
                     KQESPQYMTDFLIKMLP"
     gene            6379..8838
                     /locus_tag="VCA20_000924"
     CDS             6379..8838
                     /locus_tag="VCA20_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HEP1834771.1"
                     /translation="MLRKVKNILKKPMTLVTIIGVACVPALYNISFLTSMWDPYGRLD
                     QLPVAVVNQDQSASFQDKTLTIGDDMVDNMKESKSLDFHFVSEKDAEKGLEEGDYYMV
                     ITLPEDLSEKASSLLTNQPEPLTISYQTSKGHSFVASKMGESAMEKLKTSVSETITKT
                     YTTAVFDSMREIQTGMVEAADGSQQLTNGASQLESGSQTLSNGLTTLTTSGQALVTGA
                     NQLATGVVPYTDGVNQAATGSQTLSSGLTTYTNGVASLASGAEQLNANSSQLIAGVGQ
                     LQSGASQVEQLVTGANQLQAGLEQLASSTSLSAEQSSQIQALLTGLPQLQAAISQLND
                     SLSSIGGLAVDTSTLSSLLTEMGAQAQGLLTAAQADKTASIEALQATATYQNLTADQQ
                     AELVGALQNSPSATATVAQTILDQLSQLSQPLSSLQSLSGMATQISQLQSAIGQINTA
                     ANQALPGATTAIENLSSGLNQVNTALNQQVLPGTQTLTNGVSQLQMQLSGGASQLMSG
                     VTAYTAGVAQLAAGGAQLVANNSSIQSGGGQLTSGLATLASNSSQLVSGSGQLASGSQ
                     QLIAGADQLASGGKTLTSGITSLRTGSETLTNSLSSASQQLSVVSVEDKNAQAVSQPV
                     TLEHSDQDDVKTNGVGMAPYMVSVALMVAALSANVIFVKHIDNRSYKNRWDWAKGKLL
                     LNGIIASLAAVILYGVLRLIGIEPAHPMATLGLILLASWTFMALVTALVGWNNRFGSF
                     ASLIILLLQLGSSAGTYPIELSPRFFQVIQPYLPMTYSVSGLRQTISMVGNSSHQVWI
                     LSLFLVGFMGLGLLIYNQKDE"
     gene            complement(8888..9952)
                     /locus_tag="VCA20_000925"
     CDS             complement(8888..9952)
                     /locus_tag="VCA20_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HEP1834772.1"
                     /translation="MKKKLTIAIIIGFVSLIAGLILAGIGFFTGGITRLEEVAAPTEV
                     HKTFTDLNTIKIDFIPHSVYIKESSDNNYHVTYANSDNNMQSPLKLAEKEGTLTLSSQ
                     QESFAIEGIMQYLGERLAQRRINVFSVTIEVPKGKTLDKLEGESLHFYEYGSFVIENV
                     HIKEVDLSAGIFLDNAQIDSGKITAGYFEAVNSALRNTSVSANESSVNLIETSLENVT
                     IKNYQELNADQLTILGKNVLTPSEYSLSVTNINLIDKSLQDINLNISTQLDIKTLAEH
                     LGYHYKTLEELKQAVGDMSYFNEQAENVGIFTKDKYQTLSIKKEEDKQTLTLEKKESN
                     NSLTITSTNATINLRTPTPK"
     gene            complement(9949..10704)
                     /locus_tag="VCA20_000926"
     CDS             complement(9949..10704)
                     /locus_tag="VCA20_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEP1834773.1"
                     /translation="MTRTEYMEQLEKYLKKLPHKEYFEAISFFNEYFDEAGPERETEI
                     IEELGSPKEAASELINNMLNKQIQEEKDQQEPIQLNWKLWVGLGALSMTGLFSFFLLF
                     IMGEFIGVIPLFATLILGAFFLGRYFRNFSQTKRTLWLAILAVISLPIAIPLLLILLA
                     SLLGLVALILALIVGAFVLGVGLLTSGGYLIWEAFSLMSEGFNIFLMGFGSGLSLIGG
                     AILIYILTGFFAYWSWRLVKACFKWILKRGKRA"
     gene            complement(10691..11017)
                     /locus_tag="VCA20_000927"
     CDS             complement(10691..11017)
                     /locus_tag="VCA20_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEP1834774.1"
                     /translation="MHFPVPAVLTEFLIMAILESDDSYGYEICQTIKLIANIKESALY
                     PILKRLEQNDFLTTYSQEYQGRMRKYYSLTQLGHEELVRLKDDWDTYTNTINGIIEGS
                     VRHDKN"
     gene            11311..11691
                     /locus_tag="VCA20_000928"
     CDS             11311..11691
                     /locus_tag="VCA20_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEP1834775.1"
                     /translation="MKLYVQFMIILVFSFLGEAISTIFHLPIPGSIIGLILLFLALEF
                     KLIRLRHIHTVGNFLLANMTILFLPAAVGIMERFDAIKDYLLPIIVVILGAIFLNILV
                     IGFVVQFVKQKFEGDYIDTEGIHD"
     gene            11684..12379
                     /locus_tag="VCA20_000929"
     CDS             11684..12379
                     /locus_tag="VCA20_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002919316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEP1834776.1"
                     /translation="MTELFSNPIFGIMLSIAAYLIGMLIYRRFSHPITTPLLVATGLI
                     IVFLKMTGISYKEYYAGGSYLNMLIVPSTVALGIPLYRSFHLMKHHIRSILLGIFVAC
                     IVNTVFTALIAKWFGMDFFLAISLFPKSVTTAMAVGITDKMQGIATVTLVVVVATGIL
                     TSVLGPVFLKLLKIEDPVAVGLALGGTGHAIGTGTAIKYGHTQGAMAGLAIGITGIMY
                     VVISPIVAQIILQ"
     gene            complement(12413..13213)
                     /locus_tag="VCA20_000930"
     CDS             complement(12413..13213)
                     /locus_tag="VCA20_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEP1834777.1"
                     /translation="MGANQETLMYTLEKSIKKKADLFEHSFSAYAVRSILASLYLGLG
                     IVISLYTADKLNHVAEGLGKFSYGLMFGWGLLMILYMNAELGTSNMMYMTVASHRKTI
                     PTKTALKMLATCILFNFVGAVLVCYLVSLTLPYQHVDAHSYLFEATVAKLSKTPLTQF
                     IEGIFANIVVNIGVFTFLRIKDDAGRLISVIFIIFIFAFLGYEHVIANFSLFSLAFFA
                     NGGPVEGMTLLSVLSNFLFSGLGNYVGGGLFIGLLYSWLNNQSKLYVD"
     gene            13353..14198
                     /locus_tag="VCA20_000931"
     CDS             13353..14198
                     /locus_tag="VCA20_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEP1834778.1"
                     /translation="MDVTWTVKYITEFLATALLIIIGNGTVANVDLKGTKGNNSGWIL
                     IAIGYGLAVMMPALMFGNVSGNHINPAFTLGLAVSGLFPWAHVAQYIVAQLLGAMFGQ
                     LVVVAVYKPYFLKTENSNHILGSFSTISALDNGTKESRKAATTNGFLNEFVGSFVLFF
                     GALALTKHFFGSELVGKLIEQGYDKTVAETMTAPYTSGSIAVAHLGIGFLVMALVAAL
                     GGPTGPGLNPARDLGPRIVHALLPKSVLGENKADSKWWYAWVGVCAPIIASIAAVALF
                     KFLYL"
     gene            14328..15860
                     /locus_tag="VCA20_000932"
     CDS             14328..15860
                     /locus_tag="VCA20_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HEP1834779.1"
                     /translation="MSDVIAFGYGSSRAEMQRKRLNRHGIIAGATGTGKTVTLKVLAE
                     QLSDAGIPVFLSDIKGDLNSLVAANTKEIDPSRLEKTHYLDYSPVGYPVELWDVFGEN
                     GTPVRMTISELGPVLLTRLLGLNDTQESILNIVFSVADERGLLLIDLMDLRAMLNFVA
                     ENAAELSQYYGNIPARSVGAILRSLVVLEQQGGKIFFGEPSLDIADLMRTAEDGRGVI
                     NVLQATQLFNQPTLYSTVLLSLLSELYEVLPEVGDLDKPKMVFFFDEAHVLFKDAPKV
                     LLEKIELIVRLIRSKGVGVFFVTQNPTDIPDSVAAQLGNRIQHGLRAFTPKELKTVAT
                     VAETFRQEGDEDLAKVIQELQVGEAVVSTLQADEAPSFADRVLIYPPKSMLGTVEPSA
                     LLSVINNSPLMEKYADAVNRESAHEQILAMTEAKEAELIKKAEQTEAEKQAEKEAKAA
                     AKMEEKAQKEAAKAAQKTSQPASRKTDSMMDRFTKNLMSQVGREVGRVVTRGIMGMLK
                     GK"
     gene            15972..16580
                     /locus_tag="VCA20_000933"
     CDS             15972..16580
                     /locus_tag="VCA20_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEP1834780.1"
                     /translation="MTDQLQVLLKDYQPQPLGEKRSYAVFLPLVRSDDQWQVLYEIRS
                     ESISQPGEVSFPGGGIDEGETAEEAAIREVIEELDIQQKQIELLGEIDYLVFGRSTIR
                     CFVGRLHLDWQTLQPNDEVARFFTVPLETLQKTAPVYYQLDSQIVPDCDFPFERLRGG
                     VDYPFSHHKRSVPFYENLPENIWGMTAQFTHRFTEIVKNNLS"
     gene            16872..>18411
                     /locus_tag="VCA20_000934"
     CDS             16872..>18411
                     /locus_tag="VCA20_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein"
                     /protein_id="HEP1834781.1"
                     /translation="MSKQKVVSSLLLSTVVLGGLAFYSTTTVKAESLEPDVTSVSSGD
                     VVEKPVISNEEGGDFLAEEEIEESDAPVETLNEEIKDVTEPESVLRPRAMRSVSETSD
                     MEKIPVDDEFSDYTEEKVIGKQAELIQSSNADSKTGGDKETIKKSVTVNLLNAEEGQI
                     QWEVRFDTSAWSFKESPGGFYFVLPKGLELTEIIDNDHGKMNIINNFPKNVNVPENAA
                     KKKYRFFSEEEDQRSFNNQWGWSVGQATPDTTVNSWKSDNRFSKIYFVDQITDTTQVT
                     YTLTAKVTTSDKKSFPLMAVMKTFGYKNWSWTEVTSLGAREIILEKEKPLPPKETPAP
                     EAPLVPKEEPKKEEPQTPQAPSTPEKQPEETPKVPESPKEDTPAPPAPSVPEEPQTPP
                     APEAPTEEPKETPKPEMPEAPQAPSTPKEEAPKEEVPTPPAPSVPEEQPKETPTPEVP
                     KQEDVQPEAPKSDKVETDKLMPETKQPDMKQPKADDMPKEQKPKADEPKAEQPQMDKP
                     QMDKP"
BASE COUNT         5622 a         3399 c         3992 g         5398 t
ORIGIN      
        1 ccttataata gtggagcggc aattgctgaa tttttattta gtatttatgg aaaagatatg
       61 aaacaagatg agtttaggaa aatcattgat tatataaaag aaagaagtaa gtggaaagat
      121 gcaaattttc aaacttggag attagtttta aaaagagcat tgagttcgcc agcaacaact
      181 ttagaagaat ttattataaa tgaagagggg atagaatgaa taaaaagcga acaataagtt
      241 tttttagaca attaattaaa agtataggaa ttactcaagt ttgtatctat gtaggcatca
      301 tagtcatttt attcttattg ataggaatcc agcctttttt aatatctgaa cttttttcat
      361 ttactaaatt aactatagat tatacttatc tattcttact tttattatca tttttatctg
      421 tatcatttgt agcgtttcca aaaaattata tgttgcaaaa agtcagaaaa gtttcgaaat
      481 ctattgtttt taaggacata tcagataaaa gttattctaa gttcgttgat atgaatttag
      541 gagaagttca aaatttagca tctgaaattt catactcagc tagatcgcta cagtatgaaa
      601 gtattagttt tattttaaat gttatattga ttatctcttt gtacgcattt tcgcttataa
      661 cctatgatat ttttattggt ggcgtttata tattattttg tagtctttat ctatggctat
      721 ctattgtatt ttcaaaagat aatgttcgcc tgattcaaga tgcgttggat tcaactagtg
      781 agaccaattc cttttttgtt gattattttc aaaatgtaga ggtaattatt tctacattta
      841 gacaattttt tgaaactaag aaatacgata caattttaaa caaagagcaa aaaacatatt
      901 ttaatctaca aaaaaggata gatagtcatc agttattgtt acagttaatt ctatcagtta
      961 tgactgttgt catgattgta ttagtattaa ataaaagtag tgatggtagt atggcttata
     1021 tcctagtctt agtttactca tctattcata tgaacggatt tggcaaacaa tttttatctg
     1081 ttttagaatc cctagatcga ttagatttag ctttagataa aattgatttt ggtagattaa
     1141 ataaggtatc tatatcacat gagaatacag atacagatag tttgatttct ttaaaaaatg
     1201 ttcaattttc ccatggtgat aagaaaattt ttgataattt aacattcagc attcgaagag
     1261 gaaaatttat tttaattaga ggaaaaaatg gaacgggaaa gtcaacttta ttaaagttag
     1321 taaaagggat gattactcct gattctggaa gcgttacgca ttatttatct tcagaacaaa
     1381 cagggtattt atcacaaaag atgactttat ttgatcgtac tttgaaagag aacatgatat
     1441 accctttgga taaggttgat gatgccttgt taatgaacct aatcgcagaa ttaaaaatga
     1501 caactcttat tcaagatgta catggatggt ctgaaagaaa accaggtgat tttggggata
     1561 agttgtcagg tggggaaaag caaaagatat tgattgccag aggtataatc aatcaatatg
     1621 aagttttatt attggatgaa atagattctg ctttagattt agaaatgcag actcaattaa
     1681 tgactatttt aaaaaaatat taccaaaata aaaccgtcgt tatggtaagt cataggaaaa
     1741 tagaagagtc gttctttgat gatataattg atattgaaac gattaattga aattagaata
     1801 aacattagtt gttcgagtca attttttagt gactataaga ttattcatag tggtaataaa
     1861 attattcgat tgcattggtg aagttaattc tcaatactca caggtatgtc caaaagaata
     1921 gttagatttt attggggtag tgatatagac cacggagtaa ctttggatga actaaaaaat
     1981 ttattgataa ctaaagatat taagaaatgt cgaaaaaata cactttacat tcttcggtag
     2041 cattaaaaag cctctcttaa tttttgggga ttatgtttca tgctaactgt gtggtataat
     2101 aaaagaaatt atactaggat ttaagatgca caccctacat ggtttttgca cccccttgaa
     2161 agggaaaggt tagattgtat gataaagttg attgcgacag atatggatgg gaccttttta
     2221 gatggtcagg ggagttttga tagagagcga ttttcacggg ttttggatca actagatcag
     2281 aaaaagattc cttttgttat cgctagtggc aatggaattg ggcgattact acaattatgc
     2341 caaggatttg aaaatcgact gattttcgtg gccgataatg gtgcccatat ctatcatcag
     2401 ggtaaaacac tcattcgtcg ggctattcag caagtggaag tcgaagcaat actgcatttt
     2461 ttcaaaggcc gttgggcgga tgtatgtctg atgttgtcga acgatggaaa tatttatatg
     2521 caggctgggg caggtatgcc gtttgcaggg actgatttgc caatagaacc agctcagatg
     2581 gctgccttcc aaaatcgtgt gacatattta gacaacctta gtgcctatcc taattttgaa
     2641 cctatttata aggttggtct ctgggttcct gaagcgcgtg tggagtcgat aacggaagag
     2701 tttaatcagg ctttccaagg tcgtcttact gcagtgacaa gtggctatgg ctccattgat
     2761 atattgccag aagggcttca taaggcatgg gggttggaac aggttttgac cggtctagat
     2821 atcgaacctg aacaggtaat ggccttcggt gattcagaca atgatattga gttattgtcc
     2881 tacgttggct attcctatgc catggaaaat gcgacagaca aggtcaaggc agtagccaag
     2941 taccgagctc ccagccatct ggaggcaggg gtcatgcagg tcttggaaaa ttggttgtcg
     3001 gaaaccaagg gagagtaaaa aatccttgag tatttttata tatcctcatg ctctagtata
     3061 aaaaacaagg tctctcaagc actgagagcc ttgttttaat ttccaacgct ggtgaactgg
     3121 gtcataccat agcggaagag tttgtaattg atggtaaata agactaggac aactagtggc
     3181 agagccagtc cccacataga caagtctagg aagtagaggg ctgggaaata ggctgtaaaa
     3241 gcataaggga aaacaaatat cagcaggatt tgaatgattt ttggatagat attgagtggg
     3301 tattgagcca tctgattgag ggcgaaaatt cccatagtca aaggaaatga ttccacaatc
     3361 caaaatgcta gagcggttgg accgagttga atggctgcat agagtaggcc gatacaaatg
     3421 gcaataaaga ttagtaaggc tagttttcca agactccatt ccatacccag ttccttggaa
     3481 gcttgggcta gtgcaaggga accgatgagg gttgttccga ttccttgtgg ctggattcgt
     3541 tcacagataa gttgaaaaag tgggttcaca ggcatgagga ggaggcgttc aaattcgcct
     3601 tgtcggatgt agcgactacc aaacatccag agattgtcaa agaaaatcaa gtgaatggag
     3661 cgtccgacgg tcgctattcc atagatgaaa agcatttgac cgtagttgaa accagcgatt
     3721 tccttaatat ttccaaagag gatgttgagg aaaatcaggt agacaatttg ttcaatcaaa
     3781 gaggagaaaa gcccgatgaa gaagtccact cggaaggaca tctgggccat catgtagacc
     3841 ttgatgttat atagatagag gctgatatat ttcttcatgt taacctccaa aaatgacaat
     3901 acgacgcttg gcctgggacc agagtagagc gatgaagcag gctagaatgg gtatccatat
     3961 aacttgcaat cccaaagcag ttagatcgct ttcctgtcct agtaaaatgg aaactggaac
     4021 gttaaccgca taattatagg gaaggagttt caaaaagttt tccacagcct gtggatagaa
     4081 gctgagtggc aaaagcgctc cggaagctag attgaaaagt cccattctca gaagcatgac
     4141 tccccaggca ttgaccgtaa aaaatgctaa gaagccaaaa gccatatcca gtaattgaac
     4201 gataaacata ccgaggatgg agctgactag aaagagtaaa atagtttttc cgtcaggtag
     4261 ataaaaatcc tgatgaacaa gtatgatacc gataaatacg atggcaaact ttatcagttc
     4321 caagagctta tttccaaggt ctttgaaaaa gagagctagg ataaaggaat agggtctgag
     4381 aaattctcca gcaatgcttc cttttataat gctgtaggat aaatcttctc caagtgagaa
     4441 agaatttaaa ctggctagta gattcgcaaa gatgatatat gtagtaaagg tttgcagggt
     4501 aaagccattg acttctgatt gagtgaagaa aatggttttc cagacataga gagctaccaa
     4561 aatcttcact gccagtgaaa cgaaagtcgc tagaaagaaa gccttatact gcatagcatt
     4621 catcatggtt aggcgggtca tgtagagata tttacgcatg gataccctcc tcataaatct
     4681 gacggaccat ggtttcaatt tccaattcct tcatggtgac atcttcaatg gcataatgat
     4741 tgaaaacttg ctcaatcacc tgggcgctgg tccagacctt gctttggtag tggatttcaa
     4801 agtgaaaatc atctttacga acaaattccc agccctccaa ttgcaaatct ttttcaattg
     4861 gtttttctgt ggagaagaga atggtcttca ctgtggaaaa gcgttctttg aggatggtca
     4921 gtgaaccatc gtagaccttc ttgcccttat caatgataaa aatccgctca cagaggcttt
     4981 cgatgtcctt catatcatgg gaagtaatca aaaaagtggt ctggtattct tgattcatat
     5041 agcggatgaa ggagcgaatg gtttccttga cgctggcatc caagccgatg gttggctcgt
     5101 ccaaaaaaac aacggcaggc tggtggataa agatagccgc aaattcacag cgaacccgtt
     5161 ggccgagtga cagattacgg attggttgct tcataatatc agccaagtcc aatagatcaa
     5221 tcaagacagc caatcgctct ttgaatacct tatctggaac atcataaatt tttgcgtgaa
     5281 ggataaaaga ttcctggaca ggcaagttcc aatccaagtg agatttttgt ccaaagagaa
     5341 ctccgatttg cttattgaca gctttgcggt tttcttgtgg attaagccca ttgatgcgga
     5401 cggagccttg gtctggatag agaacacctg tcagcatttt gatggtggtg gatttaccag
     5461 aaccgttgga gccgatatag ccgatgattt ctccttttcc tacctgtaag gaaatatctt
     5521 gaacagcagg aatggccttt ttctttggct tgaaaaatgc cttgatagag ccttttaaac
     5581 cgacttcctt atcaatgatt gaataggttt ttgaaagatg ttctgcctga atcatatcat
     5641 aatctcctgt ttgaaaacgt ttgcgtaaga taaggatatt atagcatatt tcccagaaag
     5701 tggcggtaaa aagtcacctt taaggtaaca tttttattag aaaatcggtc atatattgag
     5761 gagattcttg cttaccattt ttaatccata cctgcatgat accgaagaag gcatgtgaaa
     5821 aatagatgct actgtattct ttttccagtt gtgataaatc ttcttttcct aagcgctccg
     5881 ctagatcatt ggcaacaaga agtcggactt tattgataag aaaattttga atttcttgag
     5941 ttccgttatg ggtcagtagg gcagaaagca atttttcacg atagagcaac tcgaaaattt
     6001 ctaaaaatga tgattggcga ttgccatcat aacggtcaaa tatagcctct aatttgtgaa
     6061 aaagtccctg ttgataggag tcgattaatt cgtacttatc cttataatgg gtataaaaac
     6121 tggaacgact gataccagca gtctgggcta ggtgggtggt tgtaatgtca ttaaatcctt
     6181 gtgattccag gcaggttacc atagcttcta aaattgcctg tcgagttcgt tctttccgac
     6241 tatctgttcc catattttct ccttttgaac atttttagac acagtgtcta aaaacagttc
     6301 ttctgacttg attttgaaaa tgatacttgt ataatgtatt ttatcagttt ttggacagta
     6361 tgtctaaagg agttgcttat gttacgaaaa gtgaagaata ttttaaaaaa accaatgact
     6421 ttggttacca ttattggtgt tgcctgtgtc ccagccttat ataacattag tttcctaacc
     6481 tctatgtggg acccttacgg ccgactagac cagcttcctg tagcagttgt caatcaagac
     6541 cagtcggcga gctttcaaga taaaaccctc accattggtg atgatatggt ggataatatg
     6601 aaagagagca aaagtctgga tttccacttt gtatcagaaa aggatgcgga gaaaggcttg
     6661 gaagaagggg actactatat ggtcatcacc ctaccagaag atttatcaga aaaagcatcc
     6721 agtcttttga cgaatcagcc tgaaccacta acgatttcct atcaaacatc taaaggtcac
     6781 agctttgttg cttctaagat gggcgaatca gctatggaga aattaaagac atctgtttca
     6841 gagacaatta ccaaaaccta cacgactgcc gtttttgata gtatgagaga aattcaaacc
     6901 ggcatggtcg aggcggcaga tggtagccaa caattaacaa atggagctag tcaattggag
     6961 tcaggcagtc aaacactttc aaatggactg actactttaa cgacttcggg tcaagcactc
     7021 gtgacaggag caaaccaatt ggccaccgga gtagtgcctt acacagatgg agtcaatcag
     7081 gctgcaacag gcagtcaaac actgtccagt ggcttaacca cctacacaaa tggggtagcg
     7141 agtcttgctt caggtgcgga gcaattaaat gccaattcct ctcagctcat cgctggagtg
     7201 ggacaattgc aatcaggagc aagtcaggtt gaacaactgg taacaggggc caatcaattg
     7261 caggcaggct tggagcaact cgctagttca actagtttgt ctgctgaaca gtctagtcag
     7321 atacaagcac tcttgacagg gcttcctcag ttgcaggcag ccatcagcca attaaacgat
     7381 agcttatcca gtatcggagg acttgcagta gatacctcta ctctatctag cctcttgacg
     7441 gagatggggg ctcaggctca aggattgttg acagcagccc aagctgataa aacagccagc
     7501 attgaagcct tgcaagcaac ggctacctat caaaacttga cagctgatca gcaagctgaa
     7561 ttggttggtg ccctccaaaa ttcaccttcg gcaacagcaa cagtagccca aacgattttg
     7621 gatcaattgt ctcagctcag tcaacccctt tctagcctac aaagtctttc tggcatggca
     7681 acacaaataa gccagctgca atcagctatc ggtcagatca acacggctgc caatcaagca
     7741 ttgccaggtg ctacaacagc cattgaaaac ctatcaagtg gcttgaatca ggtcaacacc
     7801 gccttgaacc agcaagttct accaggaact caaaccttga cgaatggtgt tagtcaactt
     7861 caaatgcaac tgtcaggcgg agcgagtcaa ctcatgtctg gagtaacagc ctataccgct
     7921 ggtgttgccc aattagcagc aggtggagcc cagttggttg caaacaatag tagcatccaa
     7981 tcaggaggtg gccagctaac atcaggattg gcaaccttgg catccaactc cagtcaactt
     8041 gtcagcggtt caggacaact ggcttctggt agtcagcaat taattgctgg tgcggatcaa
     8101 ttagctagtg gcgggaaaac cttgacaagt ggtattacta gcctccgcac aggtagtgaa
     8161 accctaacga attcactcag ctcagccagt caacaactat cagtcgtctc tgtagaagac
     8221 aaaaatgcac aagcagtttc tcaaccagtc accttggaac acagtgacca agatgatgtg
     8281 aagaccaacg gtgtcggaat ggctccttac atggtatcag tcgcactgat ggttgcagcc
     8341 ttatcagcga atgttatttt tgtcaaacac attgataatc gctcctacaa gaatcgttgg
     8401 gactgggcca aaggaaaact cttgctcaat ggtatcattg ccagcctagc agcagtgatt
     8461 ctgtacggtg ttcttcgttt gattggaatc gaaccagctc atccaatggc aaccttggga
     8521 ttaatcctct tagcctcttg gacctttatg gccctcgtta cagccctggt gggctggaac
     8581 aatcgtttcg gctccttcgc aagtttaatt atcttgttac tacagcttgg ctcaagcgca
     8641 ggtacctatc caattgaact cagcccacgc ttcttccaag tcattcagcc ctatcttcca
     8701 atgacctatt ctgtttcagg tcttcgtcaa accatttcta tggttggaaa tagtagtcat
     8761 caagtttgga tactgagcct cttcttagta ggctttatgg gactaggtct cttgatttat
     8821 aatcaaaaag atgaataaga ataaaaccaa ctgctccatg tgaacagttg gtttttggtt
     8881 gatgtggtta ttttggggtt ggtgttcgta gattaatggt tgcgttagtt gacgtaatgg
     8941 tcaggctatt attgctctct tttttctcca aagttaaggt ttgtttatcc tcctcttttt
     9001 tgatcgataa tgtttgatac ttatctttag tgaaaatacc gacgttttca gcctgttcgt
     9061 tgaagtaact catatcacca acagcttgct ttagttcttc aagggtttta tagtggtagc
     9121 ctaagtgttc cgcaagtgtt ttgatgtcca gctgtgtact gatattcaga ttgatgtctt
     9181 gtaagctctt atctattagg tttatatttg taacagatag gctgtattcg gaaggagtga
     9241 ggacgttttt tcctaggata gtcaattgat ctgcattcag ttcttggtag tttttaattg
     9301 taacattttc caaactggtt tcgattaaat tgactgaaga ttcatttgca gatacagagg
     9361 tatttctgag tgcagaattc acagcttcaa agtaaccagc tgtgatttta ccgctgtcaa
     9421 tttgtgcatt atcaaggaag ataccagcgg ataggtcaac ttctttgata tggacatttt
     9481 caatcacgaa actaccatac tcatagaagt gaagactctc tccctctaat ttatccagtg
     9541 tcttcccttt aggcacttca atggtgacgg agaagacatt gatacgacgt tgggccaacc
     9601 tttctcccag atactgcatg attccttcga tagcaaaaga ttcttgttgt gaagaaaggg
     9661 tcaaagttcc ctctttttct gccagtttta agggggattg catattgttg tcggagttgg
     9721 cataggtcac atgatagttg ttatcactag actctttgat gtagacgcta tgggggataa
     9781 aatcaatttt aatggtattc aaatctgtga aggtcttatg gacttccgtt ggagcagcaa
     9841 cctcttctaa gcgggttata cctccggtga aaaatccaat gccagctaga attaagcctg
     9901 caatcaggct gacaaagccg ataataatgg cgatggtaag tttctttttc atgctcgttt
     9961 acctcgtttc aagatccatt tgaagcaagc cttgaccagt cgccaagacc agtaggcaaa
    10021 aaatccagtc aagatgtaga tgagaatagc tccgccaatc aaagataaac ctgagccaaa
    10081 ccccatgagg aagatattga atccttctga cataaggcta aaggcttccc aaatcagata
    10141 gccaccgctg gtgagaagtc cgacacccaa aacaaaggca ccgacaatga gggccaagat
    10201 gagcgccacc aatcccaata gactagctag gagaatgagc aaaagtggaa tggcgatagg
    10261 tagtgaaatg acagctagga tagccaacca gagcgttcgt tttgtttggc taaaattacg
    10321 gaagtagcga ccgaggaaaa aagcgccgag gataagagtc gcaaacagag gaatgactcc
    10381 gataaactct cccatgataa agagtaggaa gaaagagaag agacctgtca tacttaaggc
    10441 tcccagacca acccagagtt tccagttgag ttgaataggt tcttgttgat ccttctcctc
    10501 ctggatttgc ttgttgagca tgttgttaat gagttcgcta gcagcttctt ttggtgaacc
    10561 aagttcttct ataatttctg tttcacgctc tggtccagcc tcgtcaaaat actcattaaa
    10621 gaagctgatg gcttcaaagt attctttgtg gggcagtttt ttgagatatt tttctaactg
    10681 ctccatgtat tcagttcttg tcatggcgga cgctcccttc tatgatgccg tttatcgtgt
    10741 ttgtataggt atcccagtca tctttgagtc ggaccaattc ttcgtgacct aactgagtta
    10801 ggctataata cttgcgcata cgaccctgat attcttggga ataagtagtc aaaaagtcat
    10861 tttgctccaa tcgttttagg atgggataga gggcggactc cttgatgttg gcaattaatt
    10921 taatggtctg acagatttca tagccataag aatcatcgga ttccaaaata gccatgatga
    10981 gaaattctgt cagcactgcg gggactggaa aatgcatagg acgctccttt cctttgttgt
    11041 tagttaggtt cggttttgat atataatgtt cgtttttata tatcaaattt tgttatatat
    11101 taaatataca cctacagaaa taaattgtca agtgattatg tgcaaaataa ataaaatagc
    11161 ttaggacaaa tttttaaggt gtaaacgttt tatagccttg aaaaataatt tcagagtagt
    11221 aaacttaata acagtactaa cattaaggga atttctggac taaaagagaa tgacctcaga
    11281 tggattagta gtatttaagg tggtgtaaag atgaaactat atgttcagtt tatgattatt
    11341 ctggtatttt catttttagg agaagctatt tcgactattt ttcatcttcc tattccggga
    11401 agtattattg gcttaatcct cctattttta gccctagagt tcaaattgat tcgtcttcgc
    11461 catattcata cggttgggaa ttttttgctg gctaacatga ccattctttt tttgcctgca
    11521 gcagtgggca ttatggagag atttgatgca attaaagatt atttacttcc tattatcgtc
    11581 gtcattttag gcgccatttt tttaaatatc ttagtaattg gttttgtcgt tcaatttgtg
    11641 aagcaaaagt ttgaaggtga ctacattgat acggagggaa ttcatgactg aacttttttc
    11701 aaatcctatt tttggtatta tgttatcaat tgcagcatat ttaattggaa tgctgattta
    11761 tcggagattt tctcatccaa tcacaacccc actgttggta gctacaggat tgattattgt
    11821 ctttttaaaa atgacaggta tttcctataa ggaatattat gcaggagggt cctatctaaa
    11881 tatgcttatt gtgccttcaa cagtagctct gggtattcct ttgtatcgtt ctttccatct
    11941 aatgaaacat cacattcgaa gtattctttt aggaattttt gttgcctgta ttgtcaatac
    12001 tgtctttact gccctaattg caaaatggtt tgggatggat ttttttctag caatctctct
    12061 ttttccaaaa tcagtcacca ctgctatggc tgttggtatt actgataaaa tgcaaggaat
    12121 agcaactgtt actcttgtag tcgttgttgc aacaggaatt ctgacaagtg ttcttggtcc
    12181 agtcttttta aagctactaa aaattgaaga cccggtagct gttggtcttg ctttgggagg
    12241 aacaggacat gccattggaa cgggtacagc gataaaatat ggacatacgc aaggagcgat
    12301 ggctggcttg gctattggga ttacaggcat catgtatgtg gtgattagtc caatagttgc
    12361 gcagattatc ttacaataaa aaactacgat tggaattcca accgtagttt ttttagtcta
    12421 cataaagttt ggattgatta ttcaaccaac tataaagaag tccgataaag agaccgccac
    12481 ccacgtagtt tcctaatcca gaaaagagga aattgcttag tacacttaat agagtcattc
    12541 cctcaactgg tccaccattt gcaaaaaagg ccagggagaa gagtgaaaag ttcgcaatga
    12601 cgtgctcata tcccaagaaa gcaaagataa agatgatgaa aataacggaa atgagacgtc
    12661 cagcgtcatc tttgatgcgt aagaaggtaa atacgccgat attcacgaca atattagcaa
    12721 aaattccttc aataaattga gtcaaaggtg tctttgaaag cttggccacc gttgcttcaa
    12781 ataggtagct gtgagcatca acgtgctgat aaggcagagt cagtgagacc aagtaacaaa
    12841 caaggacagc accgacaaag ttgaaaagga tacaggttgc caacatcttg agggctgttt
    12901 tagtaggaat cgtttttcga tggctggcta ctgtcatata catcatgttg gatgttccaa
    12961 gttcggcatt catatataaa atcatgagta gcccccaacc aaacatcaac ccatagctaa
    13021 atttcccgag tccttctgcg acatggttga gtttgtctgc tgtataaaga gaaatgacaa
    13081 tccccaatcc taaataaaga ctggctagta tcgatcgtac agcgtaggca gaaaaactgt
    13141 gttcaaacaa atctgctttt ttcttgatgc tcttttcaag tgtatacatt aatgtctctt
    13201 ggttagctcc catcctaatc tccttactga atctactact tttttgagta aacgttttct
    13261 ttttatcgaa tggctttact ttgtttatta tacttgataa aatggttgtt gtccaaaaaa
    13321 aatcaacttt tgaaaggaaa tataagaaaa atatggatgt tacatggaca gtgaaatata
    13381 tcactgaatt tttagcgact gcactcctta ttatcattgg taatggtaca gttgcaaacg
    13441 ttgatttaaa aggcacaaaa ggaaacaact ctggttggat tctcattgcg attggctatg
    13501 gtttggcagt tatgatgcca gcattgatgt ttggtaacgt ttctggtaac catatcaacc
    13561 cagccttcac acttggtttg gctgtttcag gtctcttccc ttgggctcac gttgctcaat
    13621 acattgttgc tcaattgtta ggtgcaatgt ttggtcagtt agtggttgta gcagtgtata
    13681 aaccttactt cttgaaaact gaaaattcta accatattct tggttcattc tcaactatca
    13741 gtgcgcttga taacggaacg aaagaaagcc gtaaagctgc gacaactaat ggtttcttga
    13801 atgagttcgt tggttcattt gtattgttct tcggtgccct tgctttgacc aaacatttct
    13861 ttggttcaga gttggttggt aaattgattg aacagggcta tgataaaaca gttgctgaga
    13921 caatgactgc tccatatact tcaggttcaa ttgcagttgc tcacttaggc attggttttc
    13981 tagttatggc acttgttgcg gctcttggtg gtccaacagg tcctggtctt aacccagcgc
    14041 gtgaccttgg tccacgtatc gtccatgccc ttcttccaaa atctgttctt ggtgaaaaca
    14101 aggctgattc aaaatggtgg tatgcttggg ttggtgtatg tgctccaatt atcgcatcaa
    14161 ttgccgctgt tgcactattc aaattcttgt atctataata gtgaacccga ttcgtcgggt
    14221 ttttcttttt gttttatgga tattgaggtg attcagttgt ctgctctgct attttcttaa
    14281 aagttatgct acaataaagc tagaaaaatc ggaaatgagg caagtatatg tcagatgtta
    14341 ttgcttttgg ctatggaagc agtcgagcag agatgcagcg caagcggctc aatcgccatg
    14401 gaatcatcgc aggtgcgaca ggtacaggga agacagtgac cttaaaggtc ttggcagagc
    14461 agttgagtga tgcgggaatc cctgtctttc tgtcggacat caagggtgat ttgaatagtt
    14521 tggttgcggc caataccaag gagattgatc ctagtcgtct agagaagact cactatcttg
    14581 actatagccc agtcggttat ccagttgagt tgtgggatgt ttttggggaa aatgggactc
    14641 ctgtccgcat gaccatttca gagctgggtc ctgtcctact gacacgtttg ttgggcttga
    14701 atgatactca ggagtctatc ctgaatattg ttttcagcgt ggcagacgag cgtggtcttc
    14761 tcttgattga tttgatggac ctgcgggcta tgctcaattt tgtggctgaa aatgcggcag
    14821 agctgagcca gtattatgga aatattccag ctcgttcggt tggagccatc ttgcgcagct
    14881 tggttgtttt ggagcaacaa ggtggtaaga ttttcttcgg tgagccaagc cttgatattg
    14941 ctgatttgat gcgtacggca gaagatggtc gtggggtaat caacgttctc caagctactc
    15001 agctatttaa tcagccaaca ttatattcaa cggttcttct cagcctattg tcagagctct
    15061 atgaagtctt gcctgaggtg ggcgatttgg acaagcctaa gatggtcttc ttctttgacg
    15121 aggctcatgt tctcttcaaa gatgctccaa aagttcttct tgaaaagatt gaattgattg
    15181 tccgtttgat tcgttcaaaa ggtgtaggtg tcttctttgt aacgcagaat ccgacggata
    15241 ttcctgatag cgtggcagcc cagttgggca accgcatcca gcatggtctt cgcgccttta
    15301 caccaaaaga gcttaagaca gtcgcgactg ttgccgaaac cttccgtcaa gaaggtgatg
    15361 aggatttggc caaggtcatt caggagttgc aggtaggtga agcggttgtg tctactctgc
    15421 aagctgacga ggcaccaagc tttgcggatc gagtattgat ttatcctcca aagagtatgc
    15481 tgggaacggt ggagccaagc gccctcttgt cagttatcaa taattctcct ttgatggaaa
    15541 aatatgctga cgcggtcaat cgtgagtcag cccatgagca gattttagcc atgacagagg
    15601 ctaaagaagc tgaactcatc aaaaaagcag agcagaccga ggcggaaaaa caggcagaaa
    15661 aagaagctaa ggcagccgca aaaatggaag agaaagccca gaaagaagca gctaaagctg
    15721 cacaaaaaac tagtcagcca gccagtcgca aaacggattc tatgatggac cgatttacta
    15781 agaacctgat gagtcaggtc ggacgggaag ttgggcgcgt ggtaactcgt ggtattatgg
    15841 gcatgttaaa aggaaaataa acgactaaac tgggaaggaa aattctcagt tttttatcta
    15901 gacaaagttc ttgccagata ccacaaaatt cgttaaaata aacacaaatc actagttagt
    15961 aaggagttat catgactgat caattgcagg tattattgaa agactaccag ccccaaccat
    16021 tgggagaaaa acggtcctat gcagttttct tacctctagt aaggtcagac gatcagtggc
    16081 aggtcttata tgaaattcga agcgagtcca tttctcaacc aggagaagtt tcctttccag
    16141 gtggcggaat tgatgaaggc gagacagcag aagaagctgc cattcgtgag gttatcgaag
    16201 aactcgacat tcaacagaaa caaattgagt tgctgggaga aattgattat ctcgttttcg
    16261 gtcgctcgac gattcgctgt tttgtcggtc ggcttcacct ggattggcag acccttcaac
    16321 ccaatgatga agtggctcga ttctttacag ttccactaga aaccttgcaa aaaacggccc
    16381 ctgtctatta tcaactggat tcacagattg tcccagattg tgactttccc tttgaacgtt
    16441 tgagaggtgg ggttgattat cctttcagtc atcacaaacg ctccgttcca ttttatgaaa
    16501 atctaccaga aaatatctgg ggaatgaccg cccaatttac ccatcgtttt acggaaatag
    16561 tgaaaaataa tctatcgtga aatgataggt tatttttttg gaggcaaaac cagcctttgc
    16621 gatacgtagc aacgaaaaaa atggagacaa agctcaatgt gagcaatgta tagaaaaaca
    16681 caaaaaaaac agatgcatgg cagataaaag atagctatgg ctggtatact tggtacggtc
    16741 catattttga agtaccatca tctatccagg aatgcgacaa tattcatgta aacgcattca
    16801 gtggcttgtt gtcatttcca tgtagatgag tgggaaaatg tataatctaa tattttaaga
    16861 aggagccaat tatgagcaag cagaaagttg tgtccagttt attattaagc acagtcgtat
    16921 tagggggatt agccttttat tcaacgacaa cggttaaagc agaatcgcta gaaccagatg
    16981 ttacatcagt tagttcaggt gatgttgttg agaaaccggt aatatcaaat gaagagggag
    17041 gcgacttttt agctgaagaa gagattgaag agagcgatgc tccagtagaa actctaaatg
    17101 aagaaatcaa agatgtcaca gagcctgaat cagttcttcg ccctcgtgct atgagaagtg
    17161 tttctgaaac tagtgatatg gaaaaaattc cggtagatga tgaattttcg gattataccg
    17221 aagaaaaagt aattgggaaa caggcagaac taattcaatc ttcaaatgca gattctaaga
    17281 ccggcggtga taaagagaca attaagaaat cagtaactgt aaatctttta aatgcagaag
    17341 aaggtcagat tcagtgggaa gtaagatttg acacaagtgc atggtctttt aaggaatctc
    17401 ctggtggttt ttattttgtt cttccaaaag gtttggaatt gacagaaatt attgataatg
    17461 accatggtaa aatgaatata attaataatt tccctaaaaa tgttaatgtt ccagaaaatg
    17521 ctgctaaaaa gaaataccgt ttctttagtg aggaagaaga ccagagaagt tttaataatc
    17581 aatggggttg gtctgtagga caagctacgc ctgataccac ggttaacagt tggaaatcag
    17641 acaatcgttt ttctaagata tacttcgtag accaaataac agatactacc caggtgacct
    17701 atactttaac tgctaaagtg actacgtcag acaaaaaatc attccctctt atggcagtga
    17761 tgaagacgtt tgggtataaa aattggagtt ggacagaggt tacttcacta ggtgcacgtg
    17821 agataatcct tgagaaagaa aaacctctac cacctaaaga gactccagca ccagaagcac
    17881 cattagttcc aaaggaagaa ccgaaaaaag aagagccaca aacaccacaa gcgccatcga
    17941 ctcctgaaaa gcaaccagaa gaaacgccta aagttcctga atcaccgaag gaagacaccc
    18001 cagcgccacc ggctccatca gtaccagaag agccacaaac accaccagca ccggaagcac
    18061 caacggaaga acctaaggaa actccaaaac cagagatgcc agaagctcca caagcgccat
    18121 caactcctaa agaggaagca ccgaaggaag aagttccaac accaccggct ccatcagtac
    18181 cagaagagca accaaaagaa actccaacac cagaagttcc aaaacaagaa gatgttcaac
    18241 cggaagcgcc aaaatctgat aaagtagaaa ctgacaaact aatgccagaa actaaacaac
    18301 cagatatgaa gcaaccgaag gcagacgaca tgcctaagga acaaaaaccg aaagccgacg
    18361 agccaaaggc agagcaacca caaatggaca aaccacaaat ggacaaacca c
//
LOCUS       DATUCG010000035        18349 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.036, whole genome shotgun sequence.
ACCESSION   DATUCG010000035 DATUCG010000000
VERSION     DATUCG010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18349
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.036"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            142..540
                     /gene="spx"
                     /locus_tag="VCA20_000935"
     CDS             142..540
                     /gene="spx"
                     /locus_tag="VCA20_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEP1834782.1"
                     /translation="MIKIYTVSSCTSCKKAKNWLNAHQLSYNEHNLAKEAITKEEILN
                     ILTKTENGIASIVSSKNRYAKSLDFDIEELSVNEVIDLITSNPRILKSPILIDEKRLQ
                     VGYKEDDIRAFLPRAVRNVENAQARMRAAL"
     gene            640..906
                     /locus_tag="VCA20_000936"
     CDS             640..906
                     /locus_tag="VCA20_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002885737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEP1834783.1"
                     /translation="MGFTEETVRFRLDDTDKQEISKTLTSVYRSLEEKGYNPINQIIG
                     YVLSGDPAYIPRYNDARNQIRKHERDEIIEELVRYYLKGNGIDL"
     gene            906..1325
                     /gene="ruvX"
                     /locus_tag="VCA20_000937"
     CDS             906..1325
                     /gene="ruvX"
                     /locus_tag="VCA20_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEP1834784.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIPIDEEKGEFGLER
                     LTELVEQYKVDKFVVGLPKNMNNTSGPRVEASQAYGDLLTERYKLPVDYQDERLTTVA
                     AERMLIEQADISRGKRKKVIDKLAAQLILQNYLDRTF"
     gene            1337..1657
                     /locus_tag="VCA20_000938"
     CDS             1337..1657
                     /locus_tag="VCA20_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEP1834785.1"
                     /translation="MAHHHDHEHDHNHDERELITLVDDQGNETLFEILLTIDGQEEFG
                     KNYVLLIPASAEEDENGEVEIQAYSYIENENGTEGDLQPIPEDATAEWDMIEEVFNSF
                     MEEE"
     gene            1865..2443
                     /locus_tag="VCA20_000939"
     CDS             1865..2443
                     /locus_tag="VCA20_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEP1834786.1"
                     /translation="MKDSFTERSKKLSEELERCLLADKNILVILDIMDRLNLSDCWLC
                     AGTIRNFIWNQYSFDEETDVDLVFFDENISYEETMEIESDLYQNYPAYRWELKNQVYM
                     HIHSPNTKPYQSSRDAIEKFPERCTAIGICRTERNKLEIFAPFGLDDIYAYQVRPTPH
                     FLADPNRMELYRQRILKKQWKKRCLSLTVKET"
     gene            3106..3414
                     /gene="rpsJ"
                     /locus_tag="VCA20_000940"
     CDS             3106..3414
                     /gene="rpsJ"
                     /locus_tag="VCA20_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEP1834787.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAAKIVETATRTGAQVAGPVPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            3627..4253
                     /gene="rplC"
                     /locus_tag="VCA20_000941"
     CDS             3627..4253
                     /gene="rplC"
                     /locus_tag="VCA20_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEP1834788.1"
                     /translation="MTKGILGKKVGMTQIFTESGEFIPVTVIEATPNVVLQVKTVETD
                     GYAAVQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNIEGLEVGQEITVETFAA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKGKNLAG
                     RMGGNRVTIQNLEVVQVVPEKNVILIKGNVPGAKKSLITIKSAVKAGK"
     gene            4278..4901
                     /gene="rplD"
                     /locus_tag="VCA20_000942"
     CDS             4278..4901
                     /gene="rplD"
                     /locus_tag="VCA20_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEP1834789.1"
                     /translation="MANVTLFDQTGKQAGEVVLNDAIFGIEPNQAVVFDVIISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYAYKLP
                     QKVRRLALKSVYSEKVAKNKFVAVNSLEFTATKTAEFAKVLAALSIDSKVLVILEEGN
                     EFAALSARNIPGVKVATATTASVLDIANADKLLVTQAAISKIEEVLA"
     gene            4901..5200
                     /locus_tag="VCA20_000943"
     CDS             4901..5200
                     /locus_tag="VCA20_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEP1834790.1"
                     /translation="MNLYDVIKKPVITESSMGQLEAGKYVFEVDTRAHKLLIKQAVEA
                     AFEGVKVANVNTINVKPKTKRVGRYVGRTNKVKKAIITLAADSKAIELFATADAE"
     gene            5218..6051
                     /gene="rplB"
                     /locus_tag="VCA20_000944"
     CDS             5218..6051
                     /gene="rplB"
                     /locus_tag="VCA20_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEP1834791.1"
                     /translation="MGIKVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVALKSKAGRN
                     NNGRITVRHQGGGHKRFYRLVDFKRNKDGVEAIVKTIEYDPNRSANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPEADIKVGNALPLANIPVGTVVHNIELKPGRGGELVRAAGA
                     SAQVLGQEGKYVLVRLQSGEVRMILGTCRATVGTVGNEQHGLVNLGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNQ
                     K"
     gene            6301..6582
                     /gene="rpsS"
                     /locus_tag="VCA20_000945"
     CDS             6301..6582
                     /gene="rpsS"
                     /locus_tag="VCA20_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEP1834792.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRRK"
     gene            6600..6944
                     /gene="rplV"
                     /locus_tag="VCA20_000946"
     CDS             6600..6944
                     /gene="rplV"
                     /locus_tag="VCA20_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEP1834793.1"
                     /translation="MAEITSAKATARTVRVSPRKSRLVLDNIRGKSVADAIAILKFTP
                     NKAAGIIEGVLNSAIANAENNFGLEKANLVVSEAFANEGPTLKRFRPRAKGSASPINK
                     RTAHITVVVAEK"
     gene            6957..7610
                     /gene="rpsC"
                     /locus_tag="VCA20_000947"
     CDS             6957..7610
                     /gene="rpsC"
                     /locus_tag="VCA20_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEP1834794.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRNFIKK
                     ELADASTSTIEIERAVNKVIVSIHTAKPGMVIGKAGSNVDALRAQLNKLTGKQVHINI
                     IEIKQPDLDAHLVGESIARQLEQRVAFRRAQKQAIQRAMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEALTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            7614..8027
                     /gene="rplP"
                     /locus_tag="VCA20_000948"
     CDS             7614..8027
                     /gene="rplP"
                     /locus_tag="VCA20_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEP1834795.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKQVDFGQYGLQATTSSWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEVAGVSEEIAREAFRLAGHKLPVKVKFVKREAE"
     gene            8037..8243
                     /locus_tag="VCA20_000949"
     CDS             8037..8243
                     /locus_tag="VCA20_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEP1834796.1"
                     /translation="MKLQEIKDFVKELRGLSQEELAKKENELKKELFELRFQAAAGQL
                     EQTARLNEVKKQIARIKTVQSETK"
     gene            8267..8527
                     /gene="rpsQ"
                     /locus_tag="VCA20_000950"
     CDS             8267..8527
                     /gene="rpsQ"
                     /locus_tag="VCA20_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEP1834797.1"
                     /translation="MERNNRKVLVGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEEAVII"
     gene            8552..8920
                     /gene="rplN"
                     /locus_tag="VCA20_000951"
     CDS             8552..8920
                     /gene="rplN"
                     /locus_tag="VCA20_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEP1834798.1"
                     /translation="MIQTETRLKVADNSGAREILTIKVLGGSGRKFANIGDIIVASVK
                     QATPGGAVKKGDVVKAVIVRTKTGARRADGSYIKFDENAAVIIREDKNPRGTRIFGPV
                     ARELRDGGFMKIVSLAPEVL"
     gene            9059..9364
                     /gene="rplX"
                     /locus_tag="VCA20_000952"
     CDS             9059..9364
                     /gene="rplX"
                     /locus_tag="VCA20_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEP1834799.1"
                     /translation="MFVKKGDKVRVIAGKDKGVEALVVTALPKVNKVIVEGVNIVKKH
                     QKPNSENPQGAIVEKEAPIHVSNVQVLDKNGVAGRVGYKFVDGKKVRYNKKSGEVLD"
     gene            9388..9930
                     /gene="rplE"
                     /locus_tag="VCA20_000953"
     CDS             9388..9930
                     /gene="rplE"
                     /locus_tag="VCA20_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEP1834800.1"
                     /translation="MANRLKEKYLNEVVPALTEQFNYSSVMAVPKVDKIVLNMGVGDA
                     VSNAKNLEKAAQELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVTVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKTRGMDIVIVTT
                     ANTDEESRALLTGLGMPFAK"
     gene            9945..10130
                     /locus_tag="VCA20_000954"
     CDS             9945..10130
                     /locus_tag="VCA20_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEP1834801.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCEKCGRPHSVYRKFKLCRVCF
                     RDLAYLGQIPGVTKASW"
     gene            10467..10865
                     /gene="rpsH"
                     /locus_tag="VCA20_000955"
     CDS             10467..10865
                     /gene="rpsH"
                     /locus_tag="VCA20_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEP1834802.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIATILKNEG
                     FVKNVECIEDDKQGIIRVFLKYGPNGEKVITNLKRVSKPGLRVYSKREDIPKVLNGLG
                     IAIISTSEGLLTDKQARQKNVGGEVIAYVW"
     gene            11130..11666
                     /gene="rplF"
                     /locus_tag="VCA20_000956"
     CDS             11130..11666
                     /gene="rplF"
                     /locus_tag="VCA20_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEP1834803.1"
                     /translation="MSRIGNKVITLPAGVELAQNNGVVTVKGPKGELTREFPTAIEIR
                     VEGTEVTLHRPNDSKEMKTIHGTSRANLNNMVVGVSEGFKKELEMRGVGYRAQLAGNK
                     LTLAVGKSHPDEVVAPEGITFEVPTPTQIVVSGINKEVVGQTAAYIRSLRAPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            11755..12111
                     /gene="rplR"
                     /locus_tag="VCA20_000957"
     CDS             11755..12111
                     /gene="rplR"
                     /locus_tag="VCA20_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEP1834804.1"
                     /translation="MISKPDKNKIRQKRHRRVRGKISGTAARPRLNIFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSKGTKTEQAVVVGKLVAERAVAKGISEVVFDRGGYLYHG
                     RVKALAESARENGLKF"
     gene            12130..12624
                     /gene="rpsE"
                     /locus_tag="VCA20_000958"
     CDS             12130..12624
                     /gene="rpsE"
                     /locus_tag="VCA20_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEP1834805.1"
                     /translation="MAFKDNAVEIEERVVAINRVTKVVKGGRRLRFAALVVVGDRNGR
                     VGFGTGKAQEVPEAIRKAVESAKKNMIEVPMVGTTIPHEVRSEFGGARVLLKPASEGS
                     GVAAGGATRAVIELAGIADVTSKSLGSNTPINIVRATVEGLKQLKRAEEVAALRGISV
                     SDLA"
     gene            12639..12821
                     /gene="rpmD"
                     /locus_tag="VCA20_000959"
     CDS             12639..12821
                     /gene="rpmD"
                     /locus_tag="VCA20_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEP1834806.1"
                     /translation="MAQIKITLTKSPIGRKPEQRKTVVALGLGKLNSSVVKEDNPAIL
                     GMVNAISHLVTVEEVK"
     gene            12951..13391
                     /gene="rplO"
                     /locus_tag="VCA20_000960"
     CDS             12951..13391
                     /gene="rplO"
                     /locus_tag="VCA20_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEP1834807.1"
                     /translation="MKLHELQPATGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRPGFEGGQTPLFRRIPKRGFTNINAKEYAIVNLDQLNAFEDGAEVTPVVLIEAGIV
                     KAEKSGIKILGNGELTKKLTVKAAKFSKSAEEAITSKGGSVEVI"
     gene            13405..14715
                     /gene="secY"
                     /locus_tag="VCA20_000961"
     CDS             13405..14715
                     /gene="secY"
                     /locus_tag="VCA20_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEP1834808.1"
                     /translation="MFFKLLKDALKVKLVRSKILFTIFILFVFRVGTHITVPGVNAKS
                     LEALSNLPFLNMLSLVSGNAMRNFSVFALGVSPYITASIIVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYISLVLAFVQSIGITAGFNALSGAKLTNMPLNWQTYLLIGAILTT
                     GSIIVTWLGEQISEKGYGNGTSMIIFAGIISSLPGTFHEIYIDRFVNIESSRLGESAL
                     FVAALVVLIFFVVYFTTFVQQAEYKLPIQYTKRAQGAPSSSYLPLKLNPAGVIPVIFA
                     GSITAVPTSLIQYFASQNKSAGWLLTVQEYFDYSTAKGMIVYAGLIIAFTFFYTFVQV
                     NPEKTAESLQKSAAYIHGVRPGNGTEQFLSKLLTRLAVIGALFLSFVALLPILAQNLF
                     GLSSSIAFLGTSLIIVISTSIEGIKQLEGYLLKRKYVGFLEITE"
     gene            14809..15450
                     /locus_tag="VCA20_000962"
     CDS             14809..15450
                     /locus_tag="VCA20_000962"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEP1834809.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEKFNVAHISTGDMFRAAMANQTE
                     MGILAKSYIDKGDLVPDEVTNGIVKERLVQDDIKEKGFLLDGYPRTIEQAHALDENLA
                     DLGIELQGVINIEIDPSKLVERLSGRIIHKETGETFHKVFNPPVGDYKEEDFYQREDD
                     KPESVKRRLEVNIAQGQPIIDHYRAKGLVHDIEGDQDIDLVFQAIDTVLSKLQ"
     gene            15572..15790
                     /gene="infA"
                     /locus_tag="VCA20_000963"
     CDS             15572..15790
                     /gene="infA"
                     /locus_tag="VCA20_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEP1834810.1"
                     /translation="MAKEDVIEIEGKVVDTMPNAMFTVELENGHQVLATVSGKIRKNY
                     IRILVGDRVTVELSPYDLTRGRITYRFK"
     gene            15815..15931
                     /gene="rpmJ"
                     /locus_tag="VCA20_000964"
     CDS             15815..15931
                     /gene="rpmJ"
                     /locus_tag="VCA20_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEP1834811.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPANPKHKQRQG"
     gene            15951..16316
                     /gene="rpsM"
                     /locus_tag="VCA20_000965"
     CDS             15951..16316
                     /gene="rpsM"
                     /locus_tag="VCA20_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEP1834812.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDVR
                     VKDLTSDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            16334..16717
                     /gene="rpsK"
                     /locus_tag="VCA20_000966"
     CDS             16334..16717
                     /gene="rpsK"
                     /locus_tag="VCA20_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEP1834813.1"
                     /translation="MAKPTRKRRVKKNIESGIAHIHATFNNTIVMITDVHGNAIAWSS
                     AGALGFKGSRKSTPFAAQMASEAAAKSAQEHGLKTVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            16762..17700
                     /locus_tag="VCA20_000967"
     CDS             16762..17700
                     /locus_tag="VCA20_000967"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEP1834814.1"
                     /translation="MIEFEKPTITKIDENKDYGRFVIEPLERGYGTTLGNSLRRVLLA
                     SLPGAAVTSIKIDGVLHEFDTVPGVREDVMQIILNIKGIAVKSYVEDEKKIELDVVGP
                     AEVTAGDILTDSDIEIVNPDHYLFTIADGATFKAVLTVNSGRGYVPAEGNKKDDAPVG
                     TLAVDSIYTPVKKVNYQVEPARVGSNDGFDKLTLEINTNGTIIPEDALGLSARILMEH
                     LGLFTDLTEVAKSAEVMKEAEVASDDRMLDRTIEELDLSVRSYNCLKRAGINTVFDLT
                     EKTEPEMMKVRNLGRKSLEEVKVKLADLGLGLKKDK"
     gene            17715..18101
                     /gene="rplQ"
                     /locus_tag="VCA20_000968"
     CDS             17715..18101
                     /gene="rplQ"
                     /locus_tag="VCA20_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEP1834815.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAFVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
BASE COUNT         5896 a         3424 c         3975 g         5054 t
ORIGIN      
        1 gattccaggc ttttttctaa taaaaaccga atttttaccc caaaatttaa agttttttta
       61 aaaataggtc tgcagaccga gggaatttat gatataatag tctataatgt gcttaataaa
      121 atgtagcaaa ggagtgacta catgattaaa atttatactg tatctagttg tacttcttgt
      181 aagaaagcga aaaattggtt aaatgctcac caattatctt ataatgagca taatcttgcg
      241 aaggaagcca ttactaagga agaaatccta aacatcttaa caaaaacaga gaatggtatt
      301 gcaagtatcg tatcgtcaaa aaaccgttat gcaaagagtc tggactttga tattgaagag
      361 ttgagtgtga acgaggtcat tgatttaatt acctctaacc cacgtatttt gaagagtcca
      421 atcttgattg atgagaaacg acttcaagtg ggctataaag aagatgatat acgcgctttt
      481 ttgccacgtg cagtgcgcaa tgttgaaaat gctcaagctc gtatgagagc agctctataa
      541 gatgtacaaa aggactcttt tggggttctt tttgcgcatt ttttgagaat aattaaaatt
      601 tgtgatataa tgtaaaaaat agggaaagaa ggtgtaagta tgggatttac cgaagaaacc
      661 gttcgttttc gcctagatga tacagataaa caagaaatca gcaaaacatt gactagtgtt
      721 tatcgttcgc tagaggaaaa gggctataat ccgattaacc aaatcattgg ctatgtatta
      781 agtggagacc ctgcttatat tcctcgctat aatgatgccc gcaatcagat tcgtaagcat
      841 gaacgagatg aaatcattga agaattggtg cgctactatt tgaaagggaa tgggattgac
      901 ctctaatgag aataatggga ttagacgtcg gttccaagac agttggtgta gccatttcag
      961 atccgttagg tttcactgcc caagggttgg aaatcatccc aattgatgaa gaaaagggcg
     1021 aattcggtct ggagcgtttg accgaacttg tagaacagta caaggttgat aaatttgttg
     1081 taggcttgcc gaagaatatg aataatacta gtggtccacg tgtggaagct agtcaagctt
     1141 acggtgattt attgactgag cgttataaac ttccagttga ttatcaggat gaacgtttaa
     1201 caactgttgc agcagaacgg atgctgattg aacaagcgga tatcagccga ggaaaacgca
     1261 agaaagttat cgataaatta gcagctcagc tgattttaca aaattattta gatagaacat
     1321 tttaaaggag attctaatgg cacatcatca tgaccatgaa cacgaccaca accacgacga
     1381 acgtgaattg attactttgg tagacgatca aggtaacgaa acactttttg aaatcttatt
     1441 gacgattgac ggtcaagaag agtttggtaa gaactatgtt ctcttgattc ctgcaagtgc
     1501 agaagaagat gagaacggag aagttgaaat ccaagcctac tcatacatcg agaatgagaa
     1561 tggcacagaa ggcgatttgc aacctatccc agaagacgca acagcagaat gggatatgat
     1621 tgaagaagtc ttcaacagct ttatggaaga ggaataaaaa ggtccagtgg acctttttat
     1681 catgagcctg aaaataagaa agcgaattaa ggtccagtgg acctttctat catgagctga
     1741 aaatcaaaga gtgaattgag gtccggtgga gtgaagcagt ctggggggag actgtttcag
     1801 cccgagttaa gaaacagcaa ggggaggaat gaatcatgag ccagaaaatc aaaagggagg
     1861 ccagatgaaa gatagtttta cagaaagaag taagaagctg agtgaggaat tagagaggtg
     1921 tctactggct gataaaaata ttttggttat attagacata atggatagac tgaatttatc
     1981 agattgctgg ctctgtgcgg gaactatccg taatttcatt tggaatcaat acagttttga
     2041 tgaagaaaca gatgttgatc ttgtgttttt tgatgaaaat atttcttatg aagagacaat
     2101 ggaaatagaa agcgatttat atcaaaacta tccagcctat cgatgggaat taaaaaatca
     2161 agtgtatatg catatccata gtccgaatac taaaccgtat caatcatcaa gggatgcgat
     2221 tgaaaaattt ccagaaagat gcacagcgat aggtatttgt cgaacagaaa gaaataaatt
     2281 agaaatattt gctccttttg gattggatga catttatgct taccaagtac gaccaacacc
     2341 acatttctta gcggatccta atcgcatgga attgtatcga caaagaattc ttaagaaaca
     2401 gtggaaaaag aggtgtctat cgctaacagt aaaagagact taactcccac caagtaagta
     2461 ggcaaaaaac agtatttttt tataaactaa aacaatcaac tacgggagat tatgttatgg
     2521 aatttgaaac ggtttgtttg aaggttagaa acttaggtag gctatattgc tacttcaaag
     2581 ttaaatttag aacccacatc taggctataa ttcgtaaata ggaaagtcaa aaacgtaaat
     2641 ttgatcgtct cctccatgaa gatattcata gtttgattca atcaccgagc ttgggtaatg
     2701 atgagttgct gatgttaagg gctatcctga gagattatcg aaaaaattta atcaatgacc
     2761 gacttgaaaa tatggaaaat tgataagcgg acagagtttt actggcagtc aaaaaaaata
     2821 attcgagact tacaaggata tttgaaaaaa cgttgatagt taactcaaat aaaaaataag
     2881 taatgaagaa tcctgagatc aaagttttgg gattttttga atttttgagt aaaaactctt
     2941 gcaatggcaa gtggaatctg gtagaatgtt ctagtgccgt aaaaaaatac ggctgtagct
     3001 ttgatgcaag aggttgcgac acgctcggtt gcattgccac gcaacacgtg ttggttttct
     3061 tgtggagcta gcctattatg ttaaatagac gaaaaggaga aaaagatggc aaacaaaaaa
     3121 attcgcatcc gcttgaaagc gtacgaacac cgtacacttg atacagcagc tgcaaaaatc
     3181 gttgaaactg caacacgtac aggtgctcaa gtagcaggtc cagttccgct tccaacagaa
     3241 cgtagcctct acacaatcat ccgtgcgact cacaagtata aagactctcg tgagcagttc
     3301 gaaatgcgta cacacaaacg tttgatcgac attgttaacc caactcaaaa aacagttgat
     3361 gcgttgatga aattggatct tccaagtggt gtaaacgtag aaatcaaatt gtaatcaagt
     3421 agcaaggtaa actaaaagca agtgttcata attgaacacg ggctaaactc tgtgtgaaaa
     3481 agataaactt tcctagaaac agaagtttct tcgtcaagtt tcctattttc actttgagtt
     3541 tgacgccctt tgtatcttgg tttgagcatg aaaaacgctc gttaaaaact ttttaactat
     3601 aaaataagaa aaggaatatt tttctcatga caaaaggaat cttagggaaa aaagtgggaa
     3661 tgactcaaat cttcactgaa tctggtgaat ttatccctgt tactgtcatc gaagcaactc
     3721 caaacgttgt tcttcaagtg aaaacagttg aaactgatgg ttacgcagca gttcaagttg
     3781 gttttgatga caaacgtgaa gtattgagca acaaacctgc caaaggccat gtagctaaag
     3841 ctaacacagc tcctaagcgc ttcattcgtg aattcaaaaa cattgaaggc ttggaagttg
     3901 gacaagaaat tacagttgaa actttcgcag ctggtgatgt tgttgatgta actggtacat
     3961 ctaaaggtaa aggtttccaa ggtgttatta aacgccatgg tcaatcacgt ggtcctatgg
     4021 ctcacggttc tcgttaccac cgtcgtccag gttctatggg tcctgttgca cctaaccgtg
     4081 tattcaaagg taaaaacctt gcaggtcgta tgggcggcaa ccgtgtaaca attcaaaacc
     4141 ttgaagttgt acaagttgtt ccagaaaaga acgttatcct tatcaaaggt aatgtaccag
     4201 gtgctaagaa atctcttatc actatcaagt cagcagttaa agctggtaaa taataaggaa
     4261 aggggaaatc agtcgaaatg gcaaacgtaa cattatttga ccaaactggt aaacaagctg
     4321 gtgaagtagt tcttaacgat gcgatttttg gtatcgagcc aaaccaagca gttgtatttg
     4381 atgtaatcat cagccaacgt gctagccttc gtcaaggtac tcacgcagtt aaaaaccgtt
     4441 cagcagtctc aggtggcgga cgcaaaccat ggcgtcaaaa aggaactgga cgtgctcgtc
     4501 aaggttctat ccgctcacca caatggcgtg gcggtggcgt agtcttcgga ccaactccac
     4561 gttcatacgc gtacaaactt ccgcaaaaag ttcgtcgctt ggcacttaaa tctgtttact
     4621 cagaaaaagt tgctaaaaac aaatttgtag ctgttaactc acttgaattc acagctacaa
     4681 aaactgctga atttgcaaaa gtacttgcag cattgagcat tgattctaaa gtccttgtta
     4741 ttcttgaaga aggcaacgaa ttcgcagctc tttctgctcg taacatccca ggagttaaag
     4801 ttgcaactgc aacaactgca agcgtacttg acatcgcaaa tgcagacaaa cttcttgtaa
     4861 ctcaagcagc tatctctaaa attgaggagg ttcttgcata atgaatttgt atgatgttat
     4921 caaaaaacct gtcatcacag aaagctcaat gggccaactc gaagcaggca agtatgtatt
     4981 tgaagttgac actcgtgcac acaaactctt gatcaagcaa gctgttgaag ctgcattcga
     5041 gggtgttaaa gttgcaaatg ttaacacaat caacgtgaaa cctaaaacaa aacgcgtagg
     5101 tcgttatgta ggtcgcacaa acaaagtgaa aaaagcaatc atcacattgg ctgctgattc
     5161 aaaagcgatc gaattgttcg ctacagctga cgctgaataa tcaaaggagg aattaacgtg
     5221 ggtattaaag tttataaacc aacgacaaat ggccgtcgta acatgacttc tttggacttc
     5281 gctgaaatca caacaagcac tccagaaaaa agcttgcttg ttgctttgaa gagcaaagct
     5341 ggtcgtaaca acaacggtcg catcactgtt cgtcaccagg gtggcggtca caaacgtttc
     5401 taccgtttgg tagacttcaa acgtaacaaa gatggcgttg aagcaatcgt taaaactatc
     5461 gaatatgatc caaaccgttc agcaaacatc gctcttgtac actacacaga cggtgttaaa
     5521 gcttacatca ttgctcctaa aggtcttgaa gttggtcaac gcatcgtttc aggcccagaa
     5581 gcagatatca aagttggtaa cgcacttcca cttgcaaaca tcccagtcgg tactgttgtt
     5641 cacaacatcg agttgaaacc aggtcgcggt ggtgagttgg ttcgtgctgc tggtgcatct
     5701 gcacaggttc ttggtcaaga aggtaaatac gttcttgttc gccttcaatc aggtgaagtt
     5761 cgtatgatcc ttggcacttg ccgtgctact gttgggactg taggtaacga acaacatggc
     5821 cttgttaacc ttggtaaagc aggtcgtagc cgttggaaag gtatccgtcc aacagttcgc
     5881 ggttctgtaa tgaaccctaa cgatcaccca cacggtggtg gtgaaggtaa agcaccagtt
     5941 ggtcgtaaag caccatctac accatggggt aaaccagctc ttggtttgaa aactcgtaac
     6001 aagaaagcta aatcagacaa acttatcgtt cgtcgtcgca accaaaaata atctattctt
     6061 tgttgcttag caatctatat catccgccaa ctcggtaggg tttgttatgg actgactatt
     6121 ccagcacctc agtttgattg aaatgccagt ttgacgcagt ggttgattgc agtttctagt
     6181 agcttagcta cgtcgaaact tctaatcaac tgtgcggggc aggacaacga acacgggaac
     6241 tggggttctg tccagctccc aagccgctgt ggtacatatt ttaaaggaga aaacactaaa
     6301 atgggacgta gtcttaaaaa aggacctttc gtcgatgagc atttgatgaa aaaagttgaa
     6361 gctcaagcaa acgacgaaaa gaaaaaagta attaaaactt ggtcacgtcg ttcaacgatc
     6421 ttcccaagtt tcatcggtta tacaatcgca gtttacgatg gacgtaaaca cgtacctgta
     6481 tacattcaag aagacatggt aggtcacaaa cttggtgaat ttgcaccaac tcgtacttac
     6541 aaaggtcatg ctgctgacga caagaaaact cgtcgtaaat aatttaggag gaaagcacaa
     6601 tggcagaaat tacttcagct aaagcaactg ctcgcacagt acgtgtttca cctcgtaaat
     6661 cacgtcttgt cttggataac atccgtggca aaagcgtagc agacgcaatc gcaatcttga
     6721 aattcacacc aaacaaagct gcaggcatta tcgagggagt tttgaactca gcaatcgcta
     6781 acgctgaaaa caactttggt ttggaaaaag ctaacttggt agtcagcgaa gcattcgcaa
     6841 acgaaggacc aacgttgaaa cgtttccgtc cacgtgcgaa aggttcagct tcaccaatca
     6901 acaaacgcac agctcacatc actgtagttg tggcagagaa ataaggaggt aaaaacgtgg
     6961 gtcaaaaagt acatccaatt ggtatgcgtg ttggcatcat ccgtgattgg gatgctaaat
     7021 ggtatgctga aaaagaatac gcggattacc ttcatgaaga tcttgcaatc cgcaacttta
     7081 tcaaaaaaga attggctgat gcgtcaacat caacaattga aatcgaacgt gctgtaaaca
     7141 aagttatcgt ttctatccac actgctaaac caggtatggt tatcggtaaa gctggtagca
     7201 acgttgatgc acttcgtgct caattgaaca aattgactgg taagcaagta cacatcaaca
     7261 tcatcgaaat caaacaacct gatttggatg cacaccttgt tggtgagtca atcgctcgtc
     7321 aattggagca acgtgtggca ttccgccgtg ctcaaaaaca agctatccaa cgtgcaatgc
     7381 gcgctggtgc aaaaggtatc aaaacacaag tttctggtcg tttgaacggt gctgatattg
     7441 cccgtgcaga aggctactca gaaggaactg ttcctcttca tacacttcgt gcggatatcg
     7501 actacgcttg ggaagaagct ttgacaactt acggtaaact tggtgttaaa gtatggatct
     7561 accgtggtga agttcttccg gctcgtaaaa acactaaagg aggtaaataa caaatgttag
     7621 tacctaaacg tgtaaaacac cgtcgtgaat tccgtggaaa aatgcgcggt gaagctaaag
     7681 gtggaaaaca agtagacttt ggtcaatacg gtcttcaagc aactactagc tcatggatta
     7741 caaaccgcca aatcgaagct gcccgtatcg ctatgacgcg ttacatgaaa cgtggtggta
     7801 aagtttggat caagatcttc ccacacaaat catacactgc taaagctatc ggtgtacgta
     7861 tgggttctgg taaaggtgct cctgaaggtt gggtagctcc agttaaacgc ggtaaggtta
     7921 tgtttgaagt agctggcgtt tctgaagaaa tcgctcgcga agcattccgc cttgctggtc
     7981 acaaattgcc agttaaagtt aaattcgtaa aacgtgaagc agaataagga gaagacatga
     8041 aacttcaaga aattaaagat tttgtaaaag aacttcgtgg cctttctcaa gaagaacttg
     8101 ctaagaaaga aaacgaattg aagaaagaac tcttcgaact tcgtttccaa gctgctgctg
     8161 gtcaacttga gcaaactgct cgtttgaacg aagtgaagaa acaaattgcg cgtatcaaaa
     8221 ctgtgcaatc tgaaactaaa taatagattg ggaaaggaga atttcaatgg aacgcaataa
     8281 tcgtaaagtt cttgttggac gcgtagtatc tgacaaaatg gacaaaacaa tcacagttgt
     8341 agttgaaact aaacgtaacc acccagtcta tggtaaacgt attaactact ctaaaaagta
     8401 caaagctcat gatgaaaaca atgttgctaa agaaggcgat atcgttcgta tcatggaaac
     8461 tcgcccactt tcagctacaa aacgtttccg tcttgtagaa gttgtggaag aggcagttat
     8521 catttaatca acctgaaagg agaaaattga catgattcaa acagaaactc gtttgaaagt
     8581 tgctgacaac agtggcgcac gtgaaatctt gacaatcaaa gttcttggtg gttcaggacg
     8641 taaattcgcg aacatcggcg acatcatcgt tgcttcagta aaacaagcta ctcctggtgg
     8701 tgcggttaaa aaaggtgacg ttgttaaagc cgttatcgtt cgtactaaga caggtgctcg
     8761 tcgtgctgat ggttcataca tcaaattcga tgagaatgct gcagttatca tccgtgaaga
     8821 caaaaaccct cgcggaactc gtatctttgg cccagtggca cgcgaattgc gtgatggcgg
     8881 tttcatgaaa atcgtttcat tggcaccaga agtactttaa tcaaaaagtt ttagggaatt
     8941 tgttacaaac taatgaagtc tgtgacggat ttagtagact agaaaacaaa ctaagtcccc
     9001 tggttaaatc actaaccagg gtgcccatta gggcgtaaga aattatagga gaaatcaaat
     9061 gtttgtaaaa aaaggcgata aagttcgcgt aatcgctggt aaagacaaag gcgttgaagc
     9121 tcttgttgta acagcacttc caaaagtaaa caaagttatt gttgaaggtg ttaacatcgt
     9181 taagaaacac caaaaaccaa acagcgaaaa ccctcaaggc gctatcgttg aaaaagaagc
     9241 tccaatccat gtgtcaaacg ttcaagttct tgacaaaaat ggtgttgcag gacgcgttgg
     9301 ttacaagttt gtagacggca aaaaagttcg ctacaacaaa aaatcaggcg aagtgcttga
     9361 ttaatcacga aggaaaggag aagtataatg gcaaatcgtt taaaagaaaa atatcttaat
     9421 gaagtagttc ctgctttgac tgaacaattt aactattctt cagttatggc tgtgccaaaa
     9481 gttgataaga tcgttttgaa catgggtgtt ggtgacgctg tttctaacgc taaaaacctt
     9541 gagaaagctg ctcaagaatt ggctttgatc tcaggtcaaa aaccacttat cactaaagct
     9601 aagaaatcaa tcgccggctt ccgtcttcgt gagggtgttg cgatcggtgc gaaagtaact
     9661 cttcgtggcg aacgtatgta tgaattcttg gacaaattgg ttacagtttc acttccacgt
     9721 gtacgtgact tccacggtgt accaactaag tcatttgacg gacgtggtaa ctacacactt
     9781 ggtgtgaaag agcaattgat cttcccagaa atcaacttcg acgatgttga taaaactcgc
     9841 ggtatggata tcgttatcgt tacaactgct aacactgacg aagaatcacg tgcattgctt
     9901 actggccttg gtatgccgtt tgcaaaataa gagggagaga ataaatggct aaaaaatcaa
     9961 tgatcgctaa gaacaaacgc ccagctaagt tctctacaca agcttacaca cgctgtgaaa
    10021 aatgtggacg tccacactca gtttaccgca aattcaaatt gtgccgtgta tgcttccgcg
    10081 acttggctta ccttggacaa attccaggcg taacaaaagc ttcttggtaa gataagatgt
    10141 gcgagcgttt agcaggcaac gcaaagatag gagattttac gacgaagttt acttctagga
    10201 aaaactatct tctttgctaa gcttgtagct cgaactcaat taaaacatga tactaagggc
    10261 gtgacggaca aagtgaaaat aggaactcta gcaaagaatg cttgcgttct agatagagtt
    10321 atctttttca cacaggccgt agcccgagtt caaatgttag gatgcaatag catcatacta
    10381 gcaaattcaa actttgcatt gcctatcagg cacacattaa ctagcaagtg atctaatcaa
    10441 actgctagta agaggagaaa caacaaatgg ttatgactga cccaattgca gattttttga
    10501 cacgtattcg taacgctaac caagctaaac acgaagtgct tgaagtacct gcatcaaaca
    10561 tcaaaaaagg tattgctaca atccttaaaa acgaaggttt tgtaaaaaac gttgaatgta
    10621 tcgaagatga caaacaaggc atcatccgtg tattcttgaa atacggacca aacggtgaaa
    10681 aagttatcac aaacttgaaa cgtgtttcaa aaccaggtct tcgtgtttac tcaaaacgtg
    10741 aagatattcc taaagttctt aacggacttg gtatcgcaat catctcaaca tcagaaggtc
    10801 ttttgactga caaacaagct cgtcaaaaga acgttggtgg tgaggttatc gcatacgttt
    10861 ggtaattgga tgataccttc aaatctaata atctgatttg aaggtatcat gaaaccaaac
    10921 agcatgattg cttcttaagt gcgatcatga aacctgctca ttcgaacacg ggctaaagtc
    10981 tgtgtaaaaa agataatcct tcctcgaagc taagtcttct gcgtcaggat tcctattttt
    11041 actttgactt ctttacgccc tctgtatcat gagcccccgt gaaaactagt cgctcgacgg
    11101 cttgataatt taacaggaga attaaaaata tgtcacgtat tggtaataaa gtaattacat
    11161 tgcctgctgg cgttgagctt gctcaaaaca acggcgtggt aactgtaaaa ggacctaaag
    11221 gggaattgac tcgtgaattc ccaactgcta ttgaaatccg tgtggaaggt acagaagtta
    11281 ctcttcaccg tccaaacgat tcaaaagaaa tgaagactat tcacggtact agccgtgcta
    11341 acctcaacaa catggttgtt ggtgtttctg aaggcttcaa aaaagaactt gaaatgcgtg
    11401 gtgtcggtta ccgtgctcaa ttggctggta acaaattgac acttgctgtt ggtaaatcac
    11461 atccagatga agtggttgca ccagaaggta ttacatttga agttccaaca ccaacacaaa
    11521 tcgtcgtgtc tggtatcaac aaagaagttg ttggtcaaac agcagcttac atccgtagcc
    11581 ttcgcgctcc tgagccatac aaaggtaaag gtatccgcta cgttggtgaa ttcgttcgcc
    11641 gtaaagaagg taaaacaggt aaataatagc ttgctgaggt ggcttagcca cctggctgac
    11701 tatttctcaa attgtttcgc aagaaacaga atcatacatt aagaggtgaa tattgtgatt
    11761 tcaaaaccag ataaaaacaa aatccgccaa aaacgccacc gtcgcgttcg cggtaaaatc
    11821 tctggaactg ctgctcgccc acgtttgaac attttccgtt ctaatacagg catctacgct
    11881 caagtgattg atgacgtagc gggtgtaacg ctcgcaagcg cttctactct tgataaagaa
    11941 gtttcaaaag gtactaagac agaacaagct gttgttgtag gtaaactcgt tgctgaacgc
    12001 gcggtagcta aaggtatttc tgaagtggtc tttgaccgcg gtggatatct ctatcacgga
    12061 cgtgtgaaag ctttggctga atcagctcgt gaaaacggat tgaaattcta atagggagga
    12121 cactaagaaa tggcattcaa agataacgca gttgaaattg aagaacgcgt agtagccatc
    12181 aaccgtgtta caaaagttgt taaaggtgga cgtcgtcttc gttttgcagc tcttgtggtt
    12241 gttggtgacc gtaacggtcg cgtaggtttc ggtactggta aagctcaaga agtaccagaa
    12301 gctatccgta aagcagttga atctgctaag aaaaacatga ttgaagtacc aatggttggt
    12361 acaacaatcc ctcacgaagt tcgctcagaa tttggcggcg ctcgtgtatt gttgaaacct
    12421 gcttcagaag gttctggggt tgctgccggt ggtgcaactc gtgccgtaat cgaattggca
    12481 ggtatcgcag atgtgacttc taagtcactt ggttcaaaca cgccaatcaa catcgttcgc
    12541 gcaacagttg aaggtttgaa acaattgaaa cgtgctgaag aagtggctgc acttcgtggc
    12601 atctcagttt ctgatttagc ataagaaagg agatactcat ggctcaaatt aaaatcactt
    12661 tgactaagtc tccaatcggt cgcaaaccag aacaacgtaa aacagttgtt gcacttggac
    12721 ttggtaaatt gaactcttca gttgttaaag aagataaccc agctattctt ggaatggtta
    12781 atgctatctc tcacttggta actgttgaag aagttaagta atcatacaaa taggctaagt
    12841 cgcatagaag aatctttgcg acttctagtc catttttaca taaggcgagt ttgtgaatga
    12901 agactcttgt acttcacagg cgctagcata taaaagagga gaaaataata atgaaacttc
    12961 atgaattaca acctgctaca ggttcacgta aagtccgcaa ccgtgtaggt cgtggtacat
    13021 catcaggtaa cggtaaaaca tctggccgtg gtcaaaaagg tcaaaaggct cgtagcggtg
    13081 gcggtgttcg cccaggtttt gaaggtggac aaactccatt gttccgtcgt attccaaaac
    13141 gtggctttac aaacatcaac gctaaagaat atgcaatcgt taaccttgat caattgaacg
    13201 cttttgaaga tggtgcagaa gtaacaccag ttgtgcttat cgaagctgga atcgttaaag
    13261 ctgaaaaatc aggaattaaa attcttggta acggtgaatt gacgaagaaa ttgacagtta
    13321 aagctgctaa attctctaaa tcagctgaag aagcaatcac ttctaaaggt ggctcagtag
    13381 aagtcatcta agaggtggca gcctatgttt tttaaacttt taaaagatgc cttaaaggta
    13441 aaattggtac gtagtaaaat tctatttact atctttatcc tttttgtctt ccgcgtagga
    13501 acccacatta cagtaccagg agtaaacgca aagagtcttg aagccttgtc aaaccttccg
    13561 ttcttgaaca tgttgagttt ggtttcagga aatgctatgc gtaatttctc agttttcgca
    13621 ctaggtgtta gtccttatat tacagcttcc atcattgttc aacttttaca aatggatatt
    13681 ttacctaaat ttgttgaatg gggtaagcaa ggtgaagttg gtcgtcgtaa actaaatcaa
    13741 gcaacacgct acatttcttt ggtactggca tttgttcaat cgattggtat tacagcaggc
    13801 tttaatgctt tgtccggtgc aaaattgacc aatatgccat taaattggca aacatatttg
    13861 ttgattggag caattttgac aacaggttca attattgtaa cctggctggg ggaacagatt
    13921 tctgaaaagg gctatggtaa tggtacatca atgatcatct ttgcagggat tatttcatcg
    13981 ttaccaggaa cttttcacga gatttacatt gatcgctttg ttaatattga atcaagtcgt
    14041 ttgggggagt cagcactctt tgttgctgca ctcgtcgtat taatattctt tgttgtgtat
    14101 tttacaactt ttgtacaaca agcagaatat aaattaccaa ttcaatatac aaaacgtgca
    14161 caaggagcac cttctagctc atatttaccg ctgaaattga atccagcagg agttattccc
    14221 gtaatctttg caggctcaat cactgcagtt ccaacttctt tgattcaata tttcgcaagt
    14281 caaaataaga gtgccgggtg gttattgacg gttcaggaat actttgatta ttcaactgct
    14341 aaaggtatga ttgtatatgc aggtttaatt attgccttta cattcttcta cacctttgtt
    14401 caagtaaatc ctgagaagac tgcagaaagc cttcagaaaa gtgcagccta tatccatgga
    14461 gttcgtcctg gtaatggcac tgaacagttt ttgtcaaaat tattgacaag attggcagta
    14521 atcggtgcac tcttcttgag ttttgtagct ttgctgccta tccttgcgca aaatctcttt
    14581 gggctttctt caagcattgc gttccttggt acaagtttga ttatcgtaat ctctacaagt
    14641 atcgaaggta tcaagcaatt agaaggctac cttcttaaga gaaaatatgt aggtttctta
    14701 gaaattacag aatagaatat aggttgacct agtcaacctt ctattttgtt tttagataag
    14761 tttgtcaata atggcaaatt tatgtgaaaa caaaataagg agtttgtcat gaatctttta
    14821 attatgggtt taccaggtgc tggtaaaggg acacaagcgg ccaagattgt tgagaaattc
    14881 aatgttgctc atatttcaac aggagatatg ttccgtgcag caatggccaa tcagactgaa
    14941 atgggtattc ttgccaagtc gtacattgat aagggtgacc tcgttcctga tgaagttact
    15001 aatggcattg tcaaagaacg tttggttcag gatgacatca aggaaaaagg ctttttgctt
    15061 gatggctatc ctcgtacgat tgaacaagcc catgccttgg atgaaaattt ggctgactta
    15121 ggaattgagt tgcaaggtgt tatcaatatt gagattgatc cttcaaaatt ggtagagcgt
    15181 ctcagcggtc gtattattca taaagaaact ggagagactt tccacaaagt attcaatcca
    15241 cctgttggag actacaaaga ggaagacttc tatcaacgtg aagatgacaa gccagaatct
    15301 gtcaaacgtc gtttagaggt caatatcgca caaggacagc caatcattga tcattatcgt
    15361 gcaaaagggc tggtccatga tattgaaggt gaccaagaca ttgaccttgt tttccaagca
    15421 attgatacag tactatcaaa attgcaataa actaatagat ttggcttgca ttttttatac
    15481 aaagatgata aaatagccta gtctgactta taattgttac ctctgtgttc agaggacatc
    15541 aaatcgatat ttagagaggg gttacttttg catggcaaaa gaagatgtga ttgaaattga
    15601 aggcaaagta gtcgatacaa tgccaaatgc tatgtttact gttgagttgg agaacggaca
    15661 ccaagtcctt gcaactgttt caggtaaaat ccgtaaaaac tacattcgta ttttggtcgg
    15721 tgaccgcgta actgttgagc ttagtccata cgacttgaca cgtggacgta tcacataccg
    15781 ctttaaatag tcgaaatact tggagggatt aaacatgaaa gtaagaccat cggtcaaacc
    15841 aatttgcgaa tactgcaaag ttattcgtcg taatggtcgt gttatggtga tttgcccagc
    15901 aaaccctaaa cacaagcaac gtcaaggtta agaaatagaa aggagaaaac atggctcgta
    15961 ttgctggagt tgacattcca aatgacaaac gtgtagttat ttcattaact tatgtttatg
    16021 gtatcggtct tgcaacatct aaaaaaattc ttgcagctgc aggtatttca gaagatgtac
    16081 gcgtgaaaga tttgacatca gatcaagaag atgctattcg tcgtgaagtt gatgcaatca
    16141 aagttgaagg tgacctccgt cgtgaagtta acttgaacat caaacgtttg atggaaatcg
    16201 gttcataccg tggtatccgt caccgtcgtg gacttcctgt ccgtggacaa aacactaaaa
    16261 acaatgcccg cactcgtaaa ggtaaagctg ttgcgatcgc aggtaagaaa aaataatata
    16321 ggaggtagaa aaattggcta aaccaacacg taaacgtcgt gtgaaaaaga atatcgaatc
    16381 tggtattgct catattcacg ctacatttaa taacactatt gttatgatta ctgatgtgca
    16441 tggtaacgct attgcttggt catcagctgg tgctcttggt ttcaaaggtt ctcgtaaatc
    16501 tacaccattc gcagcccaaa tggcttcaga agcagctgct aaatctgcac aagaacacgg
    16561 tcttaaaaca gttgaagtta ccgttaaagg cccaggttca ggtcgtgagt ctgctatccg
    16621 cgctcttgct gccgctggtc ttgaagtaac agcaattcgt gatgtgactc ctgtaccaca
    16681 caatggtgct cgtcctccaa aacgtcgccg tgtataatca tacattccat acacagcttt
    16741 tcgtttaaga gggagtaaga aatgattgag tttgaaaaac caacaataac aaaaattgat
    16801 gaaaataaag attacggcag atttgtgatc gaaccattag aacgtggtta cggtacaact
    16861 cttggtaact ctcttcgtcg tgtacttctt gcctcacttc caggtgctgc agtaacatca
    16921 attaaaattg atggtgtact ccacgaattc gacacagttc caggtgttcg tgaagatgtt
    16981 atgcaaatta ttcttaacat caaaggcatt gctgtaaaat cttatgtcga agacgaaaag
    17041 aaaattgaac ttgatgtagt aggtccagct gaagtaacag caggagacat tcttacagat
    17101 agtgacattg aaattgtaaa ccctgaccat tatctcttta ccattgctga tggtgccact
    17161 tttaaagctg ttttgactgt caattcaggt cgtggttatg taccagctga ggggaataag
    17221 aaagatgatg caccagtggg aacacttgct gtagattcta tctatacgcc agtgaagaaa
    17281 gtcaattatc aagttgaacc agctcgtgtt ggtagcaacg atggttttga caaattaaca
    17341 cttgaaatca acacaaatgg caccattatt ccagaagatg ctttaggtct ttctgcacgt
    17401 attttgatgg agcacttagg tctcttcact gacttgactg aagttgcaaa atctgcagaa
    17461 gtgatgaaag aagctgaagt ggcttcagat gatcgtatgc ttgatcgtac gattgaagaa
    17521 ttggatctat ctgttcgctc atataactgt ctgaaacgtg caggcattaa cactgtattc
    17581 gacttgacag agaaaactga gccagaaatg atgaaagtgc gcaatcttgg tcgcaagagt
    17641 cttgaagaag ttaaagttaa attggctgat cttggtctag gattgaaaaa agataaataa
    17701 tataggagga aatcatggca taccgtaaac taggacgcac tagctcgcaa cgtaaagcaa
    17761 tgttgcgcga tttgacaact gaccttttga tcaacgaatc aatcgttaca actgaagctc
    17821 gtgctaaaga aatccgtaaa acagttgaaa aaatgatcac acttggtaaa cgtggtgatt
    17881 tgcacgcacg tcgtcaagca gcagcatttg ttcgtaatga aattgcatct gaaaactatg
    17941 atgaagcaac tgataagtac acttctacta cagcacttca aaaattgttc tctgaaattg
    18001 cccctcgtta tgcagaacgt aatggtggat atactcgtat cctcaaaact gaaccacgtc
    18061 gtggtgatgc tgcaccaatg gcaattatcg aacttgtata agatcatcaa ttttgttgag
    18121 tgttatgatg atggaatcct attctgattc ttagtctagc tctggtctac cgctgagctt
    18181 gtctcagcgg taacactcat catgttgatt ggacgcttgt ttacgaaatt actctaagat
    18241 agaataattt cgtaagcagg cgtttttatg gctctttgtc aactgtagtg ggtagacgaa
    18301 aagctaacac ctagagagta cgaaattcgt tctctcattt ttgatgttt
//
LOCUS       DATUCG010000036        18287 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.037, whole genome shotgun sequence.
ACCESSION   DATUCG010000036 DATUCG010000000
VERSION     DATUCG010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18287
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.037"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(23..832)
                     /locus_tag="VCA20_000969"
     CDS             complement(23..832)
                     /locus_tag="VCA20_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018381403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HEP1834816.1"
                     /translation="MKEKFQMCKTYLPVVLATIGFVNGCKIIFGELGKPNGMEAKYPL
                     FLLTISLVAIYILPLLVLTYSLAKRYNISKQVIGLSWLLGLTSSISFSELGHTAIGYF
                     LLEIVKASNNFLNDWGAAISGPLAEEIGKGLTVLLVLLICRKMTLKNALVSGVIVGLG
                     FQIMEDITFVFRDMFMNKLDGFETILERVGQAGWAHWVFTLLFAIGLVALLTKNTGMS
                     KAQGVFWIGASFGIHFLFNSPFNTGIFQTVFPILSIFLGLLAYQTVEKLSE"
     gene            1206..1358
                     /locus_tag="VCA20_000970"
                     /pseudo
     CDS             1206..1358
                     /locus_tag="VCA20_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017769018.1"
                     /note="Involved in disulfide oxidoreductase activity and
                     electron transport; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
     gene            complement(1446..1589)
                     /locus_tag="VCA20_000971"
     CDS             complement(1446..1589)
                     /locus_tag="VCA20_000971"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834817.1"
                     /translation="MLQTKYSKWLLIHQYLLSVFIPFQSVQEIADNYKKPPITDGHSQ
                     SMK"
     gene            complement(1628..2470)
                     /locus_tag="VCA20_000972"
                     /pseudo
     CDS             complement(1628..2470)
                     /locus_tag="VCA20_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638074.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
     gene            2629..3246
                     /locus_tag="VCA20_000973"
     CDS             2629..3246
                     /locus_tag="VCA20_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5052 family protein"
                     /protein_id="HEP1834818.1"
                     /translation="MKLQKKIMLSVVLGLSLVSLSACQSISNWWKNTKEEWIGLEMTV
                     RTFDENSQLIDEMSGKSLSISRNEEFDSVDAEGYSNADSSVLKVTLGNYEIDHVGSSL
                     IAAEEGLEDLYAKYQTTVDIANYDRAIPLVNRMVSSLKNDFTGKAKVVLIRSQNGTPL
                     ATYVGDKVSLYASDAPKTSELLIDGKRLIIYRCDYTIYDRELLEN"
     gene            3247..3756
                     /locus_tag="VCA20_000974"
     CDS             3247..3756
                     /locus_tag="VCA20_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEP1834819.1"
                     /translation="MIDIRSARTEDAADLVAIYAPYVEKTAITFETEVPTVEAFASRI
                     EKTLEKFPYLVAVEEGKVVGYAYASSYYARAAYDWTVELSVYIKQEARGKGIGTLLYN
                     ALERELTARGFKNFLACIALPNAASIALHEKRGYEQVAHFKKVGYKFDTWHDIVWLQK
                     SLVGENDED"
     gene            3746..4399
                     /locus_tag="VCA20_000975"
     CDS             3746..4399
                     /locus_tag="VCA20_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEP1834820.1"
                     /translation="MKIEELEKRLLAVADASQAQPMKAYMKNNFDFLGVRTPDRRKVA
                     RQFFKEFKSQGIDWEFVEACWSKPYREFQYIAIDYLVTKKKDLVLTDLPRLKKLAQTK
                     SWWDSIDGLDKLVGKIVLDNPEAKQTILEWSLDDDFWLRRIAIDHQLLQKEKTDTKLL
                     EQILINNLNQTEFFINKAIGWSLRDYSKTNPEWVLVFLTKYSSQMAGLSIREASKYI"
     gene            4408..4695
                     /locus_tag="VCA20_000976"
     CDS             4408..4695
                     /locus_tag="VCA20_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HEP1834821.1"
                     /translation="MSKVYEFETLIHPVPDKGGAYVIFPYDIREEFGKGRVKVHARFD
                     GHPYDGSIVNMGVKDEAGNICYIIGVQKAIRAAIGKQAGDRVQVTIQERLE"
     gene            complement(5244..6020)
                     /locus_tag="VCA20_000977"
     CDS             complement(5244..6020)
                     /locus_tag="VCA20_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HEP1834822.1"
                     /translation="MNTRKGIIFNIQHFSLHDGPGIRTTVFLKGCPLRCPWCANPESQ
                     KRKPEPMLDAVSKKVTIMGEEKSVDDIISEVMKDIDFYEESGGGLTLSGGEIFAQYEF
                     AKAILMEAKRHDLHTAIETTAFVEHRKFVDLIQYVDFIYTDLKHYNTISHRKVTGVNN
                     NLIIQNIQYAFSIGKEIVLRIPVIPDFNNSLEDAEQFTCLFNQLKIDKVQLLPFHQFG
                     ENKYKLLNRTYAMTDVKALHPEDLKDYQAIFIKHNINCYF"
     gene            6151..6894
                     /locus_tag="VCA20_000978"
     CDS             6151..6894
                     /locus_tag="VCA20_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEP1834823.1"
                     /translation="MERLDKIIQLVSQHEKIDVNSLAEQLDVSKVTIRKDLDKLESKG
                     LLRREHGYAVLNSGDDLNIRMSFRYDVKKRIAKEAANLIADNETIMIESGSTCALLAE
                     EICKTKRNVTIITNSYFIANYVRQYDSCQIILLGGEFQKDSQVTVGPLLYKMIQFFHV
                     DKAFVGTDGYDSEHGFTGKNLMRSEVVQYMSDVAENMIVLTDSSKFTKRGTVRRFGLS
                     QVAQVITDTSISEDLVKELENARVTVRLV"
     gene            6907..7881
                     /locus_tag="VCA20_000979"
     CDS             6907..7881
                     /locus_tag="VCA20_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEP1834824.1"
                     /translation="MKQEKRRQLAKIAYLYYEEGKSQAEIAAETGIYRTTVSRMLTKA
                     KSEGIVKIEIQDFDRRLYRLEKYVQEKYGLKGLELVENSTDEDQLDLENRLAQAAADM
                     LTNLIDDGKKVGFSWGRSLSLIVDKIGNHRMKGVKFVPIAGGPSHIHARYHVNTLIYD
                     MANKFRGECSFINATIIQENQELAQGILSSKYFEELRADWKDLDVAAIGIGGRVDEKN
                     RQWLDMLTTADFQALSDEGAVGETCCRGLNQYGQAVVEDLQKRTIAISLDDLKEVPDT
                     IALAYGEEKAQAILAVLRAGYIHHLVTDERTIVKVLELDNDAHVHTNL"
     gene            8068..8391
                     /locus_tag="VCA20_000980"
     CDS             8068..8391
                     /locus_tag="VCA20_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEP1834825.1"
                     /translation="MEMIVADQLIMGLIIYAGDAKSHAYQALSLVKAGEEERAEEELE
                     LADAALLEAHNMQTKFLAQEAGGAKSDITALFVHSQDHLMTSITEINLIKEIIDLRKE
                     LKKVG"
     gene            8424..8729
                     /locus_tag="VCA20_000981"
     CDS             8424..8729
                     /locus_tag="VCA20_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEP1834826.1"
                     /translation="MVKIALFCAAGFSTGMLVNNMKIAAADAGVEVEIDAHSQGKIAD
                     YLDEIDVALLGPQVAYTLDKSKALCDEKQVPIAVIPMADYGMLDGKKVLQLALSMVS"
     gene            8742..10043
                     /locus_tag="VCA20_000982"
     CDS             8742..10043
                     /locus_tag="VCA20_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEP1834827.1"
                     /translation="MSKIDTQKIIVPIMKFVNMKGIIALKDGMLAILPLTVVGSIFLI
                     LGQLPFEGLNAAIASIFGAEWTEPFMQVYSGTFAIMGLISCFAIGYSYAKNSGVEPLP
                     AGVLSLSSFFILLKSSYIPVDGEPIGDAITKVWFGGQGIIGAIIIGLVVGAIYTTFIQ
                     RHIVIKMPEQVPQAIAKQFEAMIPAFVIFLLSMIVYIVSKMATNGGTFIEMIYDVIQV
                     PLQGLTGSLPGAIGIAFFISFLWWFGVHGQSVVNGVVTALLLSNLDANKALLAADKLS
                     LENGAHIVTQQFLDSFLIMSGSGITFGIVFAMLFAAKSKQYKALGKVAAFPALFNVNE
                     PVVFGFPIVMNPVMFLPFVLVPILAAFIVYGAISIGFMQPFSGVTLPWSTPAIISGFM
                     VGGWQGAVVQIIILAVSTIVYFPFFKIQDKIAYESEMKEPV"
     gene            10195..12639
                     /locus_tag="VCA20_000983"
     CDS             10195..12639
                     /locus_tag="VCA20_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HEP1834828.1"
                     /translation="MKTVTEVAGTSIKTEYFGSLTDRMNKYREDVLDKKPYIDAERAV
                     LATKAYQEHREKPNVLKRAYMLKEILENMTLYIEEESLIAGNQASSNKDAPIFPEYTL
                     EFVLNELDLFEKRDGDVFYITEETKEQLRSIAPFWEKNNLRSRAGVLLPEEVQVYMET
                     GFFGMEGKMNSGDAHLAVNYQKVLEQGLRGFEERVRAAKANLDLTDPASIDKYHFYDS
                     IFIIIDAVKAYADRFVALATELAEKAPTAQRRQELLEIARICAKVPYEPAETFAEAVQ
                     SVWFIQCILQIESNGHSLSYGRFDQYMYPYVKADLEAGRETEDSIVERLTNLWIKTIT
                     INKVRSQSHTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLKSVAQTHLPQPNLTV
                     RYHAGLDARFMNECIEVMKLGFGMPAFNNDEIIIPSFIEKGVLEEDAYDYSAIGCVET
                     AVPGKWGYRCTGMSYMNFPKVLLITMNDGIDPASGKRFAPAFGHFKDMKSFEELETAW
                     EKTLRHLTRMSVIVENAIDIALEREVPDILCSALTDDCIGRGKHLKEGGAIYDYISGL
                     QVGIANLSDSLAAIKKLVFEEGRLTPEELWHALETDYAGERGKEIQEMLIEDAPKYGN
                     DDDYADQLVTAAYDIYIDEIAKYPNTRFGRGPIGGIRYSGTSSISANVGQGRGTLATP
                     DGRNAGTPLAEGCSPSHNMDKNGPTSVLKSVAKLPTHEIVGGVLLNQKVNPQTLAKEE
                     DKQKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQLHPEKHRDLIVRVAGYSAFFNVL
                     SRATQDDIIGRTEHTL"
     gene            12654..13322
                     /locus_tag="VCA20_000984"
     CDS             12654..13322
                     /locus_tag="VCA20_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEP1834829.1"
                     /translation="MEFMLDTLNIEEIRKWAEVLPLAGVTSNPTIAKKEGDIDFFERL
                     HLIRDIIGPNASLHVQVVSKDYEGILADAKKIRELAPENVYIKVPVTPAGLAAMKTLK
                     AQGYQITATAIYTVFQGLLAIEAGADYLAPYYNRMANLNIDSNAVIAQLSEAIDRECS
                     ESKILAASFKNVEQVNQAFANGAQAITAGADIFEAAFSMPSIEKAVNDFADDWSAIHG
                     RYTI"
     gene            13354..14310
                     /locus_tag="VCA20_000985"
     CDS             13354..14310
                     /locus_tag="VCA20_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEP1834830.1"
                     /translation="MKQFSGLSRTIQGNHLLDTCAIDILKIGHRPLVLCAKQEYNTTV
                     ESFVKVLTCHGLIVRIAYMEEWDQNSVEEYGEVGFRHRADHIIGIGNSSVAACTKAVA
                     DLLDLPAVLIPTSPFTTLPRVSVENKKRKRPLFNESIQLIIADREQLLTPPTSELING
                     VVNTLLYLAAIKEVKRNHLFSLVDDILIRSFESVLLRGSLQAIEDFEARILSKEVQDV
                     LEVLSTCVTLDLPSDAWDVIQQIESKLSERREDLVDEPLKRNVVLYLLLTYLTQESEE
                     FQARLKWSQQLLLSANSSNLLSLEAVLKEIALEMGEDGSTRC"
     gene            14431..15525
                     /locus_tag="VCA20_000986"
     CDS             14431..15525
                     /locus_tag="VCA20_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HEP1834831.1"
                     /translation="MKVFASPSRYIQGKHVLFQGAEAIGKLGTKPLILCDDLVYGIVG
                     EKFLSYLVEEGMQVYRVAFNGEASDKEIQRVVEIGKEQASDVVIGLGGGKTIDSAKAI
                     ADLLGVPVVIAPTIASTDAPTSALSVIYSEEGAFERYIFYKKNPDLVLVDTAIICQAP
                     SRLLASGIADGLATWVEARAILQSNGTTMAGGGQTLAGIAIAQACEQTLFKYGLQAMA
                     SCEAKVVTAALENIVEANTLLSGLGFESAGLAAAHAIHNGFTALEGDIHHLTHGEKVA
                     YGTLTQLFLENRPKEELEKYIRFYQALNLPTTLEELHLADASYEDLLKVGQQATIEGE
                     TIHGMPFAISAEDVAEALMAVDYYVRSLDK"
     gene            15694..16770
                     /locus_tag="VCA20_000987"
     CDS             15694..16770
                     /locus_tag="VCA20_000987"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEP1834832.1"
                     /translation="MAYVVAVVGATGAVGAQMIKMLEESTLPIEKVRFLASARSAGKT
                     LQFKGQDIVIEETTETAFEGVDIALFSAGGSTSAKYAPYAVKAGAVVVDNTSYFRQNP
                     DVPLVVPEVNAHALDAHNGIIACPNCSTIQMMVALEPVRQKWGLERIIVSTYQAVSGA
                     GMGAILETQAQLRAVLNDGVEPKAVEANILPSGGDKKHYPIGFNAIPQIDLFTENDYT
                     YEEMKMTKETKKIMEDDSIAVSATCVRIPVLSAHSESVYIETKEIAPIDEVKAAIAAF
                     PGAVLEDDVANQIYPQAINAVGSRDTFVGRIRKDLDKENGIHMWVVSDNLLKGAAWNS
                     VQIAETLHERGLVRPTAELKFELK"
     gene            16843..17778
                     /gene="dapA"
                     /locus_tag="VCA20_000988"
     CDS             16843..17778
                     /gene="dapA"
                     /locus_tag="VCA20_000988"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEP1834833.1"
                     /translation="MSIQDLRDVKIITAMITPFKEDGSINFEVLPELIEHLLSHHTEG
                     ILLAGTTAESPTLTHEEELELFGAVQKIVNGRVPLIAGIGTNDTRDSIEFAKEVAAFG
                     GFAAGLAIVPYYNKPSQEGMYQHFKAIADASDLPIIIYNIPGRVVVEMTPETMLRLAE
                     HPNIIGVKECTSLANMAYLIEHKPEDFLIYTGEDGDAFHAMNLGADGVISVASHTNGD
                     EMYEMFTAIEQQDIRTAAAIQRKFIPKVNALFSYPSPAPVKAVLNYLGFEVGPLRLPL
                     VPCPEEDAKRIIKVVVDGDYEATKATVTGVVRPDY"
     gene            complement(18195..>18287)
                     /locus_tag="VCA20_000989"
     CDS             complement(18195..>18287)
                     /locus_tag="VCA20_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEP1834834.1"
                     /translation="LPLPPYSPEYNPIEKTWAHIKKHLRRVLPN"
BASE COUNT         5544 a         3302 c         4100 g         5341 t
ORIGIN      
        1 cgctcaaagg cggatttttt gtttattcag atagtttctc aaccgtttga taggcaagta
       61 agccaaggaa aatactcaag ataggaaaca ccgtttggaa gatacctgtg ttgaatggtg
      121 aattaaagag aaaatggatt ccaaagcttg caccgatcca aaatactccc tgtgcttttg
      181 acatacctgt atttttcgtg agaagggcta ctaagccaat ggcaaagaga agcgtgaaaa
      241 cccaatgggc ccaaccagct tgaccaacgc gttcaagaat agtttcaaag ccgtctaatt
      301 tattcatgaa catgtctcta aaaacaaagg tgatgtcttc catgatttgg aaacctaatc
      361 caacaatcac tccgcttacc aatgcatttt ttagtgtcat ttttctacaa ataagcagta
      421 caagtagaac agttagccct ttaccgattt cttctgccaa tggacctgaa atagctgcgc
      481 cccagtcatt taggaaatta ttactagctt taacgatttc gagcaggaaa tatccgatag
      541 ctgtatgtcc cagttcagaa aaagagatgc tacttgttaa gcctaaaagc cagctaagac
      601 ctataacttg tttggagatg ttatagcgtt tagctaagga atatgtcagt actagtaggg
      661 ggaggatgta aatagcgacc aaggaaatgg ttaggagaaa gagaggatat ttagcttcca
      721 tgccatttgg cttacctaat tctccaaaga taattttgca accgttgaca aatccaatcg
      781 ttgctaagac gactggaaga taggttttac acatttgaaa tttttctttc atagtttgac
      841 ctttctgtat taataaacta gtgtatggat acattttatc aaaaataagt acatttcgtc
      901 tattattcac ctatttttta tctaaacctt ctcagttatt gtaatgtttt ttcctgcaac
      961 taggactcca taatagagcc gtaacggaat tgtgtctcaa attttctttt ttgctagact
     1021 aaggtttaaa aaataaaagg agtatgttat ggaacaatat gatttattgg tcattggttt
     1081 tggatggaaa gaccttagca ggtaagctac cagtaccagt agctgggaaa aaggttgcct
     1141 tggtggaggg aaatcctgct atgtttggcg ggacttgtat caatattggc tgtattccaa
     1201 ccaagactct ctttggtcgc aattcagagg agctgattaa cttgattgtt atggccattg
     1261 acaataagat tccttatacc tacttcaaaa cacaaatctt cacccatccg actatggctg
     1321 agaatttgaa cgatgtcttt tcactcatcg aaaattaaca ctaatcggcg tcaacttgcc
     1381 ttgatatact caagtataat ctgcgacttc gtttactaga ctagtgtcaa gatcatcgag
     1441 tgaccttatt tcattgactg actatgaccg tccgtaatgg gcggtttttt ataattatct
     1501 gcgatctctt ggacagattg gaatgggata aagactgata atagatattg atgaatcaac
     1561 aaccatttcg aatacttggt ttgtaacata ataaaagcct gtcaatgcag gcttgtttca
     1621 gtatgtgtta ctggtggtgc tgattcgttt caatttggtc tactttgaaa aatgcagcaa
     1681 gtgccacaca gaaactaaag cagagcatga gaacggttat ttcaccagag ttagatgtta
     1741 gtccttcgtt gaatggagta ttcaagaaaa agtgtgctaa gacagccatc agcatccaga
     1801 aaagtccttg ttttttcgac atgccactat tttttgagac gagagcgacc aaaccgactg
     1861 cgaatgcaag ggagaaggcc cagtgagttc caccagcgtg aagcatacgt tcgataagag
     1921 tggcgaagcc atctgtttta gatttaaaga tttgttggaa gacatagaga ccgtcttcaa
     1981 taatttggaa actcaaacct acaatcatac cgctgactac ggcatttttt agagtaagtt
     2041 ttttgagcaa taggagtaca agtagtacaa ctagtccctt accaaactct tcatggaagg
     2101 gggcggtgac agctgctccc cagttttgga taaatgtttg aggtggtttg acgatgaata
     2161 accaaaatgc tcctaaaagc gtttgaccaa gaccagagaa ttagaaacta gctgttatac
     2221 ccaaaatcca actgagatgg ataacttttt tagaaatatc aaaacgtttc gctatataac
     2281 gaatgataag aatcataggg aagacataaa gtgcaactaa ggaaatagtt agaaagaaga
     2341 gaggatactt tccgtccatt ccatctggtt tcccaaaaaa ggtaagaaga actttacagc
     2401 cagagaaaaa actccaaacg gctagaaaaa taagtagctt cattttgtgc ttagcacaga
     2461 attgtttcat aaaaatgact tcctttatat taatttagta gatgtcagtt atccctaccg
     2521 tgctatagtt tatcatagcc atcttcttat tatcttaggt ctatctattt tttctcggtt
     2581 tttgtattcc gtgatataat aagtaaaata tgttggagga ctattaatat gaaattgcaa
     2641 aagaaaatca tgttatctgt tgttcttggt ttgagccttg ttagtttgtc tgcctgtcaa
     2701 tcaatttcaa attggtggaa aaataccaag gaagaatgga ttggtttgga aatgacagtt
     2761 cggacatttg atgagaattc tcagctaata gatgaaatgt ctggtaagtc cttatcgatt
     2821 tcgcgtaacg aggagtttga ctcggtggat gccgaaggct attccaatgc agattcgtct
     2881 gttttgaaag tcacgcttgg gaattacgaa attgaccacg taggctcatc tttgattgcg
     2941 gcagaagaag gtttggaaga cttgtatgcg aaatatcaga ccacagtaga tattgcaaat
     3001 tacgatcgtg caattccgct tgttaaccgt atggtatcta gtctcaaaaa tgattttaca
     3061 ggtaaggcaa aggtggtttt gattcgttcg caaaacggaa ccccacttgc tacttatgta
     3121 ggtgataaag tgtctcttta tgcatctgat gcacctaaga cttctgaact cttgattgat
     3181 ggcaagcgat tgattatcta tcgttgtgat tatacgattt atgacagaga attattggag
     3241 aactaaatga tagacattcg ttccgcaagg acagaggatg cggcagacct tgtggcaatt
     3301 tatgctcctt atgtagaaaa gaccgctatt acctttgaaa cagaagtacc gactgtggaa
     3361 gcctttgcaa gtcgaattga aaagaccttg gagaagtttc cctatctggt cgcagtagaa
     3421 gaaggtaaag ttgtgggtta tgcctatgct tcatcctatt atgcccgtgc ggcttacgat
     3481 tggacggtgg aattatccgt atatatcaag caagaagcgc gcggaaaagg aatcggaact
     3541 cttctctaca atgctttgga aagggaactg acagcgcgtg gttttaaaaa tttcttagcc
     3601 tgtatcgccc taccaaatgc agcctctata gcactccatg agaagagggg ctatgaacag
     3661 gtagctcatt tcaaaaaagt tggttataaa tttgatacct ggcatgatat tgtctggctc
     3721 caaaaatctc ttgtaggtga gaatgatgaa gattgaagaa ttagaaaaac gtttgttggc
     3781 tgtggcggat gccagtcagg cccagcctat gaaggcttat atgaaaaaca attttgactt
     3841 tctgggtgtg cgaactcctg accgccgaaa agtggctagg caatttttca aagaatttaa
     3901 gtctcagggc attgactggg agtttgtaga ggcttgctgg tctaagccct accgtgaatt
     3961 tcaatatatc gccattgatt acctggtcac caagaaaaaa gacttggttt taactgattt
     4021 accacgcttg aaaaaattag cccagaccaa gtcttggtgg gatagtattg acggattaga
     4081 taaactagtc ggtaagattg ttttggacaa tccagaggcc aagcaaacaa tcttggaatg
     4141 gagtttggat gacgatttct ggttgagacg gattgccatt gaccaccagc ttctccaaaa
     4201 agaaaagaca gatacgaaac tgttggaaca aatcctaatc aataatctca atcagactga
     4261 attttttatc aataaggcaa ttggctggag tttgcgggat tattccaaga ccaatcctga
     4321 atgggtacta gtttttctta ccaagtacag ctcacaaatg gcaggcttgt ccattcgcga
     4381 agccagtaag tatatctagg aggagccatg tccaaagtct atgaatttga aacccttatc
     4441 catccagtac cagataaggg cggtgcttat gtcatatttc cctatgatat acgagaagag
     4501 tttggtaagg ggagggtaaa ggtacatgcc agatttgatg gtcatcccta tgatggctcc
     4561 attgtcaata tgggagttaa ggatgaagca ggcaatatct gttatattat tggcgttcaa
     4621 aaggctattc gagcggctat tggaaaacag gctggtgata gagtacaagt gactattcaa
     4681 gaacgactgg agtaatcagg cttgcaaatc cttgagatta tggtagaata gattcatgcg
     4741 atgagccgag tagtgacttt gtcactttcg acgctaggga ggtcttcatt taattgaaac
     4801 gcaggagcgg accttgaata gttgtgtgaa ccttctattc tcatcggaaa cgatgccctg
     4861 gtccaccttt atggtgggct tttttgtata ctaagtgagg ctgggcaaaa agccctggcc
     4921 cacttctcag tgttcgtgtc aacatctcag cgcagtggtt gattggcttt aacagtccag
     4981 tggactgtta aaggttggag ataggatttg cgaagcaaat ctcagcaatt cgtgttttac
     5041 actccaaatc taacctaatc aactgtgcgg gggtgggagt gaaacagtct ggggatagac
     5101 tgtttcagct caacaactta aaacgaaaaa ttgttgacga actctttttg acttagtcga
     5161 gttctttccc actcccattt cagtatgtga tttctatagg aatgctattg gctcagccga
     5221 ggaagttttt gtatttctca aagttagaaa taacagttga tattatgttt gatgaagatt
     5281 gcttgatagt cttttaaatc ttctggatgg agagccttga catcggtcat agcataggtt
     5341 cgattgagaa gcttatactt attttctcca aattgatgga aggggagtaa ttgaactttg
     5401 tcaatcttga gttgattaaa gagacaggtg aattgttcag catcttcgag ggaattatta
     5461 aagtcaggga taacagggat acgtaggacg atttctttcc cgatggaaaa ggcgtattgg
     5521 atattttgta tgataaggtt attgttgacg ccggtgactt ttctatgaga aattgtattg
     5581 taatgtttta aatcagtata gataaagtca acgtattgga taaggtcaac aaatttgcgg
     5641 tgttctacga aagcggtggt ttcaatagcg gtatgaaggt cgtgtctttt tgcctccata
     5701 agaatagctt tcgcaaactc atattgggca aaaatttctc cgccagacag tgtgagccca
     5761 ccaccagatt cttcataaaa atcaatgtct ttcatgactt cggatatgat gtcatcaaca
     5821 gatttttctt ctcccatgat ggtcactttt ttactaacag catcaagcat gggttcaggt
     5881 tttcgttttt gagattctgg atttgcacac caaggacaac gtagggggca acctttcaag
     5941 aaaaccgtcg tacgtatacc aggaccgtca tggagcgaaa aatgttgaat gttaaaaata
     6001 atgccttttc gtgtattcat gctctttctc cttttcgatt aatttgattt tatcacacaa
     6061 aagaaagttt tacaattacg aacgtaagtt aaaagttgca aatagttgaa aaagagtcaa
     6121 aatttgatag aataatgatg aggtgatgaa atggaacgat tagataaaat tatacaactg
     6181 gtatcacaac atgaaaaaat agatgtcaat agtttggcgg agcaactgga cgtttctaag
     6241 gtaaccatcc gtaaagattt agataagttg gaatcaaaag gtttattacg cagagaacac
     6301 ggctatgctg ttctaaatag tggtgatgac cttaatattc ggatgtcctt tcgctatgac
     6361 gtaaaaaaac gaattgcaaa agaagcagca aatcttattg ccgacaatga aacgattatg
     6421 atagagtctg ggtctacttg tgctctcttg gcagaagaaa tttgcaaaac caagagaaat
     6481 gtgaccatca taacaaattc ttattttata gccaattatg tgcgacaata tgatagctgt
     6541 cagattattt tattgggtgg tgagtttcaa aaagattccc aagtaacagt tgggccgctc
     6601 ttgtataaaa tgattcagtt tttccatgtt gataaggctt ttgtaggaac tgacggctat
     6661 gatagtgagc atggttttac aggtaaaaac ttaatgcgaa gcgaagtagt acagtacatg
     6721 tctgacgttg ctgaaaacat gattgtgtta acagattcta gtaaatttac aaaacgaggg
     6781 acagtacgca gatttggttt aagtcaagtt gctcaggtta ttacagatac gtcgatttct
     6841 gaagatcttg tcaaggaatt agaaaatgct agagtcacag taaggctagt ttaggttggg
     6901 ggcagtatga agcaagaaaa aagaaggcag ttggcaaaaa tagcctatct ttactacgaa
     6961 gaggggaaaa gccaagctga gattgctgct gagacaggaa tttatagaac gacagtcagt
     7021 agaatgttga ccaaggctaa gtcagaaggc attgtaaaaa tagaaattca agattttgat
     7081 aggcgcctat atcgactaga aaaatatgtt caagaaaaat atggattaaa aggtttagaa
     7141 ctggttgaaa acagcaccga tgaggaccag cttgatttag aaaatcgtct ggcacaggct
     7201 gcagccgata tgcttaccaa tctaattgat gatggaaaga aagttggttt ttcatggggg
     7261 agaagcctca gtcttattgt agataagata gggaatcatc gtatgaaagg tgtcaaattt
     7321 gttccaatag ctggcggtcc aagtcatatc cacgcacgtt atcatgtgaa tactctgatt
     7381 tacgatatgg ccaataaatt tcgtggagaa tgtagtttta tcaatgcgac gattatccag
     7441 gagaatcaag aacttgctca aggtattctt tcttctaaat atttcgagga gctacgtgct
     7501 gactggaagg acttagatgt agcagcaata ggtattggcg gtcgagtcga tgaaaaaaat
     7561 cgtcaatggt tagatatgtt gacgacggct gattttcaag ccttatcaga tgaaggtgca
     7621 gtcggtgaaa cctgctgtcg tggtctgaat caatacggtc aagctgtcgt tgaggatttg
     7681 caaaagagga ccattgccat ctctttagat gatttaaaag aagttccaga taccatagct
     7741 cttgcatacg gggaggaaaa ggctcaagcc attttagctg tattgcgagc tggctacatt
     7801 catcatctgg taacagatga acgaaccatc gtaaaagttt tagaactgga taatgatgcg
     7861 catgtccata caaatttgta agagagtact tcaatcgctt aaaggtggtt gaagtatttt
     7921 ttatcccaaa aatgcacaaa agtgcagaaa taaattaaaa cggaaaatat tctataaata
     7981 tatttacgct ataaagagaa aatgatatga tttacttacg aaagaaatat aataacttac
     8041 aaataaaaca aatcggagga aaaagaaatg gaaatgattg ttgcagatca attgatcatg
     8101 ggattgatta tttatgctgg agatgcgaaa agtcacgcct accaagcttt gtcgcttgtc
     8161 aaagcaggtg aagaagagcg agcagaagag gaattagaat tggcagatgc agcattatta
     8221 gaagctcaca atatgcagac aaaattctta gcacaggaag ctggtggagc aaaatctgat
     8281 attacagcac tatttgtcca ttcgcaagat catttgatga catctattac agagatcaat
     8341 ttgattaaag aaatcattga tttgcggaaa gaactaaaaa aagttggcta aaacaaaaaa
     8401 attatattta ggaggaaacc gaaatggtta aaatcgctct attttgtgcc gctggttttt
     8461 ctacaggcat gttggtaaac aacatgaaga ttgcagcagc agacgcaggt gtcgaagtag
     8521 aaatcgacgc gcattcccaa gggaaaattg cagattacct tgacgaaatc gatgtagctt
     8581 tactgggacc acaagtagca tatacgcttg ataagtcaaa agcgctctgt gatgaaaagc
     8641 aagtccctat tgccgtgatt ccaatggcag actacgggat gttggacggg aaaaaagttc
     8701 ttcaattggc actcagcatg gtttcataaa ggagtatggt catgtccaaa attgatacac
     8761 aaaaaattat tgtcccaatc atgaaatttg tgaatatgaa agggatcatc gctctaaaag
     8821 atggtatgtt ggctatctta cctttaacag tagttggtag tattttctta attttggggc
     8881 agttgccttt tgaaggctta aatgctgcaa tagcttctat atttggagcg gaatggactg
     8941 aaccgtttat gcaagtctat agcggaacct ttgcgattat gggcttgatt tcttgttttg
     9001 cgatcggcta ttcttacgcc aaaaatagtg gagttgagcc tctccctgcg ggcgtactgt
     9061 ccttgtcttc attctttatc ctcttgaaat catcttatat tccagttgat ggagaaccaa
     9121 tcggtgatgc catcacaaaa gtatggtttg gtggtcaagg aattatcggt gcgattatta
     9181 ttggtttggt agtgggcgct atctatacga cttttattca gcgtcatatt gttatcaaga
     9241 tgcctgaaca agtaccgcaa gctattgcca agcagtttga agctatgatt ccagcttttg
     9301 tcatcttttt gctatctatg attgtctata ttgtttccaa gatggcgaca aacggtggta
     9361 cattcattga aatgatttac gatgtgattc aagtgccact acaaggtttg actggttcat
     9421 taccaggtgc gattggaatt gctttcttca tttccttcct ctggtggttt ggagtacatg
     9481 gccagtcagt tgtcaatggt gtcgttactg ctttactatt gtcaaacctc gatgccaata
     9541 aggccctttt agcagcagat aaattgtcac tggaaaatgg cgcccacatc gtgacccaac
     9601 aattcttaga tagtttcttg attatgtcag gttcaggtat taccttcggt attgtttttg
     9661 caatgctatt tgccgctaaa tcaaaacaat ataaggctct tggtaaggta gcggcctttc
     9721 ctgcactctt taacgtaaat gaaccagtcg tctttggttt tccaattgtg atgaatcctg
     9781 taatgttctt accatttgtt ttggtaccga tcttagcggc tttcattgtc tacggtgcaa
     9841 tctcaatagg ctttatgcaa ccattttcag gtgtgaccct accatggtct accccggcaa
     9901 tcatttctgg ttttatggtg ggtggttggc aaggtgcagt tgttcaaatc attattttag
     9961 ctgtctcaac tattgtttat ttcccattct tcaaaatcca agataagatt gcctatgaaa
    10021 gtgaaatgaa agaaccagtt tagtcaatga tgtcagacaa aagagttgca caaatgtgca
    10081 gccagcttcg gaaatcttag ctctcactag catcaattga caataccccc tattacagga
    10141 tatacttaat ttacaaacga aacaaaatta agtttcgaaa gaaagggtaa aactatgaaa
    10201 acagtgactg aagtagctgg cactagcatt aaaactgagt attttggaag tctgactgat
    10261 cggatgaaca aatacagaga agatgtcctt gataagaagc cctatattga cgcggaacgg
    10321 gctgttttag caacaaaagc ctatcaagaa catagagaga agcccaatgt attaaaaagg
    10381 gcctatatgc tcaaggaaat attggagaac atgacgcttt atattgaaga agaaagtctg
    10441 attgcaggta accaagcttc ttcaaataaa gatgctccca tcttcccaga atataccttg
    10501 gagtttgtat tgaatgaatt ggatttattc gaaaaacggg atggggatgt cttctatatt
    10561 accgaggaaa ctaaggagca gttgcgcagc atcgctccat tctgggaaaa gaataatctt
    10621 cgttcacgtg caggtgtctt gcttccagaa gaagtccaag tttatatgga aactggcttc
    10681 tttggtatgg aaggtaagat gaactctggc gatgcccact tggctgtcaa ttaccaaaag
    10741 gtattggaac aaggtttgcg tggttttgag gagcgagttc gtgcagcaaa ggctaatctc
    10801 gatttgacgg atcctgcaag tattgataaa tatcatttct atgactcaat ctttatcatc
    10861 attgatgcgg tgaaagcata cgcagatcgt tttgtcgcct tggctactga attggcagag
    10921 aaagcaccaa cagctcaacg ccgtcaagaa ctactggaga ttgcacggat ttgtgcgaag
    10981 gtgccgtatg aacctgctga aacatttgca gaggcagtgc aatctgtctg gtttatccag
    11041 tgtatcttgc aaatcgagtc caatggacat tctctatcct atggacgttt cgaccaatac
    11101 atgtatccgt atgtgaaagc tgatttagaa gcaggacgtg aaacggaaga cagcatcgtc
    11161 gaacgtttga ctaacttgtg gattaagacc attacgatca acaaggttcg tagtcaatca
    11221 catactttct catctgctgg tagtccacta taccaaaatg taactattgg tggacaaaca
    11281 cgtgataaga aggatgctgt caatccactt tcttacttag tattgaaatc tgttgcccaa
    11341 acacacctgc cacagccaaa cttgactgtt cgctatcatg caggcctaga tgctcgcttc
    11401 atgaatgaat gtattgaagt catgaaactt ggttttggta tgcctgcctt caacaacgat
    11461 gaaattatca ttccatcctt tatcgagaaa ggtgtcttgg aagaagatgc ctatgactac
    11521 agtgcgattg gctgtgtaga aacggctgtt cctggtaaat ggggataccg ttgtacaggt
    11581 atgagctata tgaacttccc gaaagtttta ttgattacca tgaatgatgg tatcgatcct
    11641 gcatcaggca aacggtttgc accagccttt ggtcacttca aagatatgaa atcctttgaa
    11701 gagcttgaaa cggcatggga aaagacgctt cgtcatctaa cacggatgag tgtcatcgtt
    11761 gaaaatgcaa ttgacattgc tttagaaaga gaagtgccag acattctctg ctcagctctg
    11821 acagatgact gtatcggtcg tggcaagcac ctgaaagaag gtggagcgat ttacgattac
    11881 atttctggtc tgcaggttgg cattgccaat ttatctgatt ctctagcagc aatcaaaaaa
    11941 ttagtctttg aagaaggacg attaacacca gaagaattgt ggcatgcttt ggaaacagac
    12001 tatgctgggg agcgtggtaa ggaaatccaa gaaatgttga ttgaggacgc accaaaatat
    12061 ggtaacgatg atgattatgc agaccaattg gtaacagcag cgtatgatat ttatatcgat
    12121 gaaattgcca aatatccaaa cactcgcttt ggtcgtggtc cgattggtgg tatccgttat
    12181 tcaggtacat catccatttc agccaacgtt ggacaggggc gtggtacctt ggcaactcca
    12241 gatggtcgaa atgcaggtac accgttggca gaaggttgtt ctccatcaca taatatggat
    12301 aaaaatggac cgacatccgt tttgaaatca gttgccaaat taccaacaca tgaaattgtt
    12361 ggaggcgttc tgctcaatca gaaggtcaat ccacaaacac ttgccaaaga agaagacaaa
    12421 caaaaattaa ttgccctctt gcggacattc tttaaccgct tgcatggcta tcatattcag
    12481 tacaatgttg tctctcgaga aaccttgatt gatgcacaac tacatcctga aaaacaccgt
    12541 gatttgattg tccgtgtagc aggttattca gcattcttca atgtattgtc gagagcaaca
    12601 caagacgaca tcattggtcg tacagaacat acattgtaaa agaggttatt attatggaat
    12661 tcatgctgga caccttaaat atagaggaaa tacgaaaatg ggctgaggtt ctaccccttg
    12721 cgggggtgac ctcaaaccca acgattgcga agaaagaagg tgacattgac ttttttgaac
    12781 gtttgcacct tatccgtgac atcatcggtc caaatgcttc actacatgtg caagttgtgt
    12841 caaaggatta tgaagggatt ttggcagacg ctaaaaaaat tcgtgaattg gcaccggaaa
    12901 atgtctatat taaggttccg gtaacgcctg ctggtttggc tgctatgaaa acattgaaag
    12961 cgcaaggtta tcagattaca gcaacagcga tttataccgt ttttcagggc ttgttagcta
    13021 ttgaagcggg tgcagattac ttagcgccgt actacaatcg catggccaat ctgaatattg
    13081 attcgaacgc ggtcatcgct cagttatcag aagcgattga tagggagtgt tcggaaagta
    13141 aaattttagc ggcgtcattc aaaaatgtgg agcaagtaaa ccaagcattt gcaaatggag
    13201 cgcaggctat tacggcaggt gcagatatat ttgaagcagc cttttctatg ccatccattg
    13261 aaaaggcggt aaatgatttt gcagatgatt ggtctgccat tcatggaagg tatactatct
    13321 aaacatttct atattataaa ataagggggc actatgaaac aattctcagg tctttcacgc
    13381 actatccaag gaaatcatct tttagacact tgtgcgattg acattttgaa aataggtcat
    13441 cgcccccttg ttttatgtgc aaaacaggag tataatacga ctgtagaaag ttttgtgaaa
    13501 gttttaactt gtcatggact tattgttaga atagcctata tggaggagtg ggaccagaac
    13561 tcggttgaag aatatgggga agttggtttc cgacatcgtg cagaccatat tattggcatt
    13621 ggtaatagtt ctgtggcagc ttgtacaaag gctgttgcag atttactaga cttgccagct
    13681 gttcttatac cgacttcacc gtttaccact ctacctcggg tatcggtgga aaataagaaa
    13741 agaaagcgac cattgtttaa tgagtccatc cagctaatta tagcggatag ggagcaatta
    13801 ttgacaccac ctacctcgga gttgataaat ggagtggtca atactcttct ttatctagca
    13861 gccataaaag aagtaaaaag aaatcatcta ttcagcttgg tagatgatat tcttatcaga
    13921 agttttgaaa gcgttttatt aagaggtagc cttcaagcta tagaggattt tgaagctcgg
    13981 atattatcaa aagaagtaca agatgtctta gaagttcttt ctacttgtgt aacattagac
    14041 ctgcctagtg atgcttggga cgtgattcaa caaatagaat caaagctcag tgaaagaaga
    14101 gaggatttag tggatgagcc attgaaacga aatgttgtcc tctatttgct tctgacctat
    14161 ttgactcagg aatcagaaga atttcaagcc aggctgaagt ggagtcaaca gttgcttttg
    14221 tctgccaact ctagcaatct attgtcgctg gaggcagttt taaaggaaat tgcactagag
    14281 atgggagaag atggctcgac acgttgctag taggcatcgc tgtacaatgg caatatttaa
    14341 aatacgggct catgcctctt gctttcggca atgctaactg tcgtttattg ctcaatataa
    14401 cactatctat tatttcaagg aggaattctt atgaaagtat ttgcaagtcc atctcgctat
    14461 attcaaggta aacatgtcct tttccaaggt gcagaagcta tcggaaaatt gggaacaaag
    14521 cctttgattc tttgtgatga tttagtatat gggattgtcg gtgagaagtt tttgtcctat
    14581 ctcgttgaag aagggatgca agtctatcgt gttgctttta atggagaagc atctgataag
    14641 gaaatccaac gcgtcgtaga aatcggaaaa gaacaggcta gtgatgtcgt aatcggccta
    14701 ggtggtggca agacgattga ctcagccaag gcgattgctg atttactagg agtgccagtt
    14761 gtcattgctc caacaattgc ttcaacagat gctccgacat ctgctttatc tgtcatttat
    14821 tctgaagaag gggcgtttga acgctatatt ttctataaga aaaatccaga tcttgtcttg
    14881 gtagatacag cgattatttg tcaagcaccg tcacgtcttt tggcttctgg tattgcggat
    14941 ggcttggcga cgtgggtaga agcgcgtgct attttacaaa gcaatggaac aacaatggca
    15001 ggaggaggtc agacacttgc tggtatcgct attgcacaag cctgtgaaca aacactcttt
    15061 aaatacggac ttcaagccat ggccagctgt gaagccaagg ttgtgacagc tgctttagag
    15121 aatattgttg aagccaatac cttgctaagt ggtttaggtt ttgaaagtgc aggtttggct
    15181 gcggcgcatg cgatacacaa tggattcacc gccttggagg gagacattca ccatttgaca
    15241 catggtgaaa aagtggctta tgggaccttg acccagctct tcttggaaaa ccgtccgaaa
    15301 gaagaattgg aaaaatacat ccgtttctat caagccttga atcttccaac aactttggag
    15361 gagttacatt tggctgatgc tagctatgaa gatctattga aagtcggcca acaagcgacg
    15421 attgaaggag aaacaattca cggtatgcca tttgctattt cagcagaaga cgttgcagaa
    15481 gctctgatgg ctgttgacta ctatgttcgc tcattagata aataagaaga gcattcacag
    15541 ttgagtatat ccatctaaac ttgtgaatag aggttcgaag taattgcctt gggaaattct
    15601 ttctcagggc ttttcatttg aaaaaattat gatataattg ttaaatataa aatatttaga
    15661 aaattttgaa gaattgactt aaaggagatt tctatggcct atgtagtagc tgttgttggt
    15721 gccactggtg cagttggtgc ccaaatgatt aaaatgttgg aagagtcaac acttccaatc
    15781 gagaaagtac gtttccttgc ttctgcccgt tcagcaggca agaccttgca gtttaagggg
    15841 caggatattg tcattgaaga aacaacagaa acagcctttg aaggagtgga tattgccctc
    15901 ttctcagctg gaggctctac atctgccaag tacgctcctt acgctgtaaa agcaggagct
    15961 gtagtagttg acaatacctc ttacttccgt caaaatccag atgttcctct ggttgttcct
    16021 gaggtaaatg ctcacgcgct tgatgcccac aatggtatca ttgcttgtcc aaactgctca
    16081 accattcaaa tgatggttgc cttggagcct gttcgtcaga aatggggctt ggagcggatc
    16141 atcgtatcca cttatcaggc agtatctggt gcaggtatgg gagctatctt ggaaacgcaa
    16201 gctcaattgc gtgcagtctt gaatgatggc gtggaaccca aagcggtaga agcgaatatt
    16261 cttccttctg gtggcgataa gaagcactac ccaatcggtt tcaatgctat tccgcaaatc
    16321 gacctcttca ctgaaaatga ctacacttat gaagagatga agatgacaaa agagactaag
    16381 aaaatcatgg aagatgacag cattgccgtt tctgcaacct gcgtccgtat tccagtcttg
    16441 tcagctcact ctgagtctgt ttacattgaa actaaagaaa ttgcaccaat tgatgaggtg
    16501 aaagcagcca ttgcagcctt tccaggggct gttttagaag acgatgtggc taatcaaatt
    16561 tatccgcaag cgattaatgc tgttggtagt cgcgatactt ttgttggtcg tatccgtaaa
    16621 gatttggaca aggaaaacgg tattcacatg tgggttgttt ctgacaactt gctcaaaggt
    16681 gctgcatgga actctgttca aatcgctgaa acccttcatg agcgtggtct agttcgtcca
    16741 acggcagaat tgaagtttga attgaaataa ttttattaac caaaagtaca caaaaggctg
    16801 cactcagcct tttttagcaa caatcagtaa gagaggaagc ctatgtctat tcaagattta
    16861 cgtgatgtaa aaattattac agcaatgatt acccctttca aagaagatgg ttcgattaat
    16921 tttgaagttt tacctgagtt gattgagcat ctgttgtccc atcacacaga agggatttta
    16981 ttagcaggaa caactgccga aagtccaact ctgacacacg aggaagaact ggaactattt
    17041 ggtgctgtgc aaaaaattgt caatggtcgt gttcccctaa ttgcaggtat cggtacaaac
    17101 gatacgcgcg actcgattga gtttgccaaa gaagtggcgg cattcggcgg ctttgcggca
    17161 ggtcttgcca ttgtgccata ttacaacaaa ccctctcaag agggaatgta tcagcacttc
    17221 aaggctattg cggatgcctc tgatttacca atcattatct acaatattcc aggtcgtgtt
    17281 gtcgttgaaa tgacgccaga aaccatgtta cgtttggctg agcatccaaa catcatcggt
    17341 gttaaagagt gtacaagcct tgctaacatg gcttacctaa ttgagcataa accagaagac
    17401 ttcttgattt atacaggtga ggatggcgat gctttccatg caatgaactt gggagcagat
    17461 ggtgtgattt ctgttgcatc tcacacgaat ggtgatgaga tgtatgaaat gtttacagcc
    17521 atcgaacaac aagatattcg aacagcagcc gctattcaac gcaagttcat tccgaaagtc
    17581 aatgccctct tctcatatcc aagtcctgca ccagttaagg cggttcttaa ttaccttgga
    17641 tttgaagttg gtccactccg cttgccgttg gttccatgcc cagaagaaga tgccaaacgc
    17701 attatcaaag tagtcgttga cggcgattac gaagcaacca aagctactgt gacaggagtc
    17761 gtacgaccgg attattaaaa acgtccagtg gacgttttta acccgagcct gaaaatacga
    17821 cagcgaggct tgtttggggg atacgaataa accggtggtc tattcgtacc tgagcctgag
    17881 aacaaaaagc gaagtacatc agctggtcac caagaaatac gaaagtgacc atagaagccc
    17941 cgagcctaga aatgcgaaag agaggacagt caggggagtg actattttaa cgtgagttag
    18001 agtataaaga gtgaggagtt tagaaaaaca ttaatataga gtagcttctg gctatcttcc
    18061 tcgttttatt taagtttaca caagcacatc agttatctgg tgtgcttttc agttgaaagc
    18121 cttttcgatt tttggcattc ttgatatagt caactgaaac aagagcagga tgaaactgcc
    18181 tcaagaaaag tatctcaatt tggcaatact cttctgaggt gttttttgat atgagcccat
    18241 gttttctcga taggattata ctctggtgag taaggaggaa ggggtaa
//
LOCUS       DATUCG010000037        18110 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.038, whole genome shotgun sequence.
ACCESSION   DATUCG010000037 DATUCG010000000
VERSION     DATUCG010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18110
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.038"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            <1..581
                     /locus_tag="VCA20_000990"
     CDS             <1..581
                     /locus_tag="VCA20_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEP1834835.1"
                     /translation="VLKKAVLDKILANCKEHNILPSYAITVLHFEGLWGTSAVGKVDN
                     NWGGMTWTGQGNRPSGVTVTQGSARPSNEGGHYMHYASVDDFLTDWFYLLRSSGSYKV
                     SGAKTFSEAVKGMFKVGGAVYDYAATGYDNYLVGMSSRLKAIESENGSLTRFDATSNN
                     VHSVDPDKISVDIDGIEVTINGVVYKLEKKPV"
     gene            766..951
                     /locus_tag="VCA20_000991"
     CDS             766..951
                     /locus_tag="VCA20_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEP1834836.1"
                     /translation="MTERELKKIAKKQGFSKTNFGKGSHEVWKHPDGRTVTIPKPKEK
                     DYRPGTLNNILKVLYGE"
     gene            1011..1415
                     /locus_tag="VCA20_000992"
     CDS             1011..1415
                     /locus_tag="VCA20_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEP1834837.1"
                     /translation="MKYNYLALFEADKENGGYSISFPDFPGAFSEADNLSEAIFNARE
                     VLEIYTIMFEDEGKEFPKPSSFKALASNLASDDDVIQAISVDTELVRERERSKIVNKT
                     VTLPSWLVEVGKENKVNFSQLLQKAIREELQV"
     gene            complement(1665..2879)
                     /locus_tag="VCA20_000993"
     CDS             complement(1665..2879)
                     /locus_tag="VCA20_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEP1834838.1"
                     /translation="MSNKMKQAIQLLFFSSLIGVGAGIITTLFGKILLGIGDLRAEYF
                     TFLIPFLPLAGLLIVFIYQKWGREVQAGMGLVFKAGQGEKAQISPVLIPLIISTTWLS
                     HLVGVSVGREGVAVQLGASLSHWLQKHGFTHLPKDMITKIGMAAGFAGLFQTPLAASF
                     FAIEVLIVGQYSWTSLPYCLVAAFTASTTSHLLGLEKFSHAITTTSFHFIDSFKWLLI
                     AICFGFIGNLFAWFLAQAKSISTRWLPNPYIKIAIMGVGLTVLLFFFHQGRYAGLGTN
                     LIDASLAGEQVFAYDWLLKLLFTCLCLAAGFQGGEVTPLFAIGASSGAVLAALLGLPT
                     ELVVALGYCAVFGAATNTLLAPLFISYEVFGANILPYAIPVLAIAYLINKKQTIYGQQ
                     LRKFNNAKKPII"
     gene            complement(2869..3147)
                     /locus_tag="VCA20_000994"
     CDS             complement(2869..3147)
                     /locus_tag="VCA20_000994"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEP1834839.1"
                     /translation="MNLDCIREQINTIDCQLVELLEKRMELVDQVTVYKRATGKPVLD
                     TSRENAVLERVGKLVQKDDYRSAIQATFSDIMAQSRTYQSSKLANHEQ"
     gene            complement(3229..3498)
                     /gene="rpsN"
                     /locus_tag="VCA20_000995"
     CDS             complement(3229..3498)
                     /gene="rpsN"
                     /locus_tag="VCA20_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEP1834840.1"
                     /translation="MAKKSKMARYQRQLELIERYADLRKSLKEKGDYQALRKLPRDSN
                     PNRLKYRDRTDGRPHAYMRKFGVSRITFRELAHLGQLPGVKKASW"
     gene            complement(3636..4097)
                     /locus_tag="VCA20_000996"
     CDS             complement(3636..4097)
                     /locus_tag="VCA20_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEP1834841.1"
                     /translation="MALAKIVYASMTGNTEEIADIVASKLEELGLEVQVHECTTIETE
                     EILDADLIVVASYTYSYGGDGELPDEIVDFYADLADLDLTGKVYGVCGSGDTFYDDFC
                     SAVDDFDVMLGSRGATKGAENVKVDLAAEDEDIVNLEQFATDLVTKLDTMN"
     gene            4192..5136
                     /locus_tag="VCA20_000997"
     CDS             4192..5136
                     /locus_tag="VCA20_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEP1834842.1"
                     /translation="MTIYSKIIEKIHEYETIIIHRHKRPDPDAIGSQVGLQKLLQKNF
                     PEKTIKVTGFEEPNLAWMAGMDVVADQDYQGALVIVTDTANTARVDDDRYAQGDFLIK
                     IDHHPNEEPYGDLLWVNTEASSCSEMITELAIQSQLELDGETARLLYAGIVGDTGRFL
                     YPATSSRTFEIVACLRQEDFDFARMSRQMDTIDFKIAKLTGYVYDNLEVDEHGAARVI
                     LTQDILKKYGVTDADTSFIVGAPGRIGTVQSWGIFVEQTDGNYRVRLRSKYLPINEIA
                     KRHDGGGHPLASGANSYSLSENEQIYQEIQEVVRNASK"
     gene            5123..6151
                     /locus_tag="VCA20_000998"
     CDS             5123..6151
                     /locus_tag="VCA20_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEP1834843.1"
                     /translation="MQASSRSLRLMGTIIETRIWHPQAEPILDQVEELLYLYKDRFSA
                     NDLTSELMEVNLNAGVQAVPVADDLYELIKLGKEHSLAQGSFLNITIGPFVQSWRIGF
                     SDAKLPTKEMISEKLQLINPRDIELDDEEQTVYLKKEGMAIDLGALAKGYVADKIVDF
                     LKRIGVAAGLINLGGNVLTFGQAPHNADGYWRIGIQEPQKPRGENALVLKIGEESVVT
                     SGIYERTLTVNGKTYHHILSPETGYPMESQLASLTIVSKQSVDGEIWTTRLFGQEITR
                     IYKMVEETDGIEAVLIGLDGRILVTSGLSEKVLAGKERISDSLGSPSRGGFGARVTSD
                     LASGASVL"
     gene            6231..6512
                     /locus_tag="VCA20_000999"
     CDS             6231..6512
                     /locus_tag="VCA20_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEP1834844.1"
                     /translation="MARKESMAERNFKMKKDIHPEYRTVVFMDTTTGYKFLSGSTKKS
                     NETVEFEGETYPLIRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLK"
     gene            complement(6615..7757)
                     /locus_tag="VCA20_001000"
     CDS             complement(6615..7757)
                     /locus_tag="VCA20_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEP1834845.1"
                     /translation="MIKQYQLKDGSVRYSYIAYVGIDPLTGKEKRVKKSGFKTQKEAR
                     IAESQLLLKVEQDGFFDKPDRITFIEVYKIWLEHYKNTVKASTYARQKAQADLHIIPA
                     FGACYVDKISLPMCQKQAQEWFKGYKKYANFIGMTKMVLDFAVNLGYIHDNPMKKIIK
                     PRKSSEVDEEEKKKENFYSREDLQKFLDCVAKEDKEEIACIFRLLAFTGMRKSEVLAL
                     RWKDLDFFASRLSVSQIVAYGENNSIVYQTPKTKKSKRTITLDPITVSILKKWEKTRQ
                     FLNFPQRVKPTDLVFPAETGNAHSFDYINYNLRCILKKYDLPYITPHGFRHTHCSLLF
                     EAGASIKEVQERLGHEDIKTTMNIYAHVTENTKEKTAEKFAQFLGM"
     gene            complement(8768..8950)
                     /locus_tag="VCA20_001001"
                     /pseudo
     CDS             complement(8768..8950)
                     /locus_tag="VCA20_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027462.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            9105..9293
                     /locus_tag="VCA20_001002"
     CDS             9105..9293
                     /locus_tag="VCA20_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP1834846.1"
                     /translation="MLLTEDHARKVRRKRADLMLTKKATAEKLGIKPQALTKVEQGNY
                     DAPRRIYESVINWLLEDV"
     gene            9611..9901
                     /locus_tag="VCA20_001003"
     CDS             9611..9901
                     /locus_tag="VCA20_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834847.1"
                     /translation="MTTLEERYCIERRAFLGQRATRLELLERVIYLELMLMDMADSTL
                     DTDFPEMAVTTTLDHIADFGDEALYLLESLDHDGYYKALWEHTKDTGANNGK"
     gene            9891..10091
                     /locus_tag="VCA20_001004"
     CDS             9891..10091
                     /locus_tag="VCA20_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834848.1"
                     /translation="MASKQLEALLERANKSDEELDYITDYLASLNNEAIETTLAGKFE
                     AVSRFIWEIQDYLQEKLKEKTQ"
     gene            10088..10330
                     /locus_tag="VCA20_001005"
     CDS             10088..10330
                     /locus_tag="VCA20_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834849.1"
                     /translation="MTMIDKNTVLFTYGEDNEHKQTVGTFLELVKNEDVEKLYSIGEA
                     LESEGKSLGKELNEIAGTFENLIDSLLTTLEGENTK"
     gene            10327..10548
                     /locus_tag="VCA20_001006"
     CDS             10327..10548
                     /locus_tag="VCA20_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834850.1"
                     /translation="MSKKQTYKEFKKWVDDHVNMAEAMGLDENAEVLHDMVEHNAQAW
                     VQLQEMLEEHTLHYQPRGKRKRGKQHGDK"
     gene            10535..10852
                     /locus_tag="VCA20_001007"
     CDS             10535..10852
                     /locus_tag="VCA20_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834851.1"
                     /translation="METNNIVTINSAELIHLTTYAFYMKHISKSLQNFTKELEKTLEP
                     NNVAYLTLAWAKINEESERAIEILEPKTSKEDYKAVMEELETLKNTQYTTEDIVLMTW
                     EGF"
     gene            10864..11271
                     /locus_tag="VCA20_001008"
     CDS             10864..11271
                     /locus_tag="VCA20_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834852.1"
                     /translation="MTKTPFTQELLQQIYEDNGLVSFDLLLERLKGWTIEGIKARFNQ
                     WRHRGIISYSLLNDEIDEFQFLKTKREEKQEITEGRKLKLDEYFKQVLATADIINKPT
                     ASDTNRLKAIQLQQQALTEIPDDIYKEFSEVYA"
     gene            11403..11666
                     /locus_tag="VCA20_001009"
     CDS             11403..11666
                     /locus_tag="VCA20_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834853.1"
                     /translation="MAEALLSESASLELVRGALVLAREMAKDEIERLNKKPLRQGEVM
                     ELYGFDHKYMKYLKSCGLRSRRQGKSIYYDVRDVDEILEKLKE"
     gene            12029..12346
                     /locus_tag="VCA20_001010"
     CDS             12029..12346
                     /locus_tag="VCA20_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEP1834854.1"
                     /translation="MWKGVKMDLKGNDKRIYSLIGVGIEKAITARHIAQQTNLDKRTV
                     RECVRRLIIKHKIPIIGNRKGNHKGYFIPANHSELMAGIGALEKQIEEEKKRLEVLLE
                     AEV"
     gene            12359..12826
                     /locus_tag="VCA20_001011"
     CDS             12359..12826
                     /locus_tag="VCA20_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834855.1"
                     /translation="MKQYFKQFEERLQVAEEKLDILSDWHIAKGHKEATKIAEECRTA
                     ITTLWMEFYRLSEAYNEAYNEAYNEAEAGHEEFYQANVDYLLGELRKHDNMLLENAIS
                     IGKGRPNYLLFDYLDREQRIFENPDNLVPAPIGNIWHYIRSLIIKDQKERGIL"
     gene            13043..13243
                     /locus_tag="VCA20_001012"
     CDS             13043..13243
                     /locus_tag="VCA20_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834856.1"
                     /translation="MNIKEQLKIIEREEPELFDVIFACITGKIPKEEVDYFLSLSDEK
                     DRAEWVKQQIVKRKNIEREKDV"
     gene            13236..13808
                     /locus_tag="VCA20_001013"
     CDS             13236..13808
                     /locus_tag="VCA20_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834857.1"
                     /translation="MYSIVEVTIKRGEQILLDLLKDNPTRAIMQNDFFRFFYYLPFGG
                     YITPFSLNNITLKVFEKVPAPKGWELIRDYPISIMKTSLLAKLEDIEKYTLDQQRQAS
                     GLYLEGWVFDTITNGIYTKEETAIFIRQMYLHGYDFEQVISLFTAIVKRSSLASYFLD
                     MMKRLYFAEATGNTIAYIINQQKQDTKIIS"
     gene            13823..15355
                     /locus_tag="VCA20_001014"
     CDS             13823..15355
                     /locus_tag="VCA20_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEP1834858.1"
                     /translation="MGSITQYERQITRETPYNARDIRLRTFKGWQGKVFQFIQHKQEE
                     RYRKLLEDGLDDKQAQAEIKKPVPPLIVAQYMAELLTVCRIESDEGTKPVYFYNPDEG
                     LYINDREFLKDFISIIEPRHNEKRASDCIYHLTRKAPHRQAENAPHLIIVGNGIYNRK
                     TRTLEPFTETRVFTHKIQTNYNPHAQSPTIKGWEFKDWLLDLFNGDKELYQLSLQLLN
                     AVVRGESYAKMFWFVGEGGTGKGTLQELFINLIGRQNIASIKITDLDVNNRFTLAQAI
                     GKQAIIGDDVQAGAVIRDTSKLFSLVGGDTVTVEKKGKDAYSTFIKTVVIQSTNTLPK
                     IRGDYHAIRRRMVILPFNKHFKGKPNRAIKNDYITRPSVLEYVLKTVIDLDFKDFIEP
                     SKSIDLLDEYQEAIDPVLAFSQNLFTNLHSTFIPNDWVWWYFTGFADYHNFQHNFSSQ
                     GFHTHFPQYLPEDWEKLAYPISLPRGADLPKGFYPKADQPHYLPLYAPIKGKAQRGYK
                     KK"
     gene            15673..15858
                     /locus_tag="VCA20_001015"
     CDS             15673..15858
                     /locus_tag="VCA20_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834859.1"
                     /translation="MKQTNVENFRTRTDGKYKGMMEGHAQWLENLERHYFPNKSASEY
                     DNLNAREEEGKEANECS"
     gene            15845..16387
                     /locus_tag="VCA20_001016"
     CDS             15845..16387
                     /locus_tag="VCA20_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEP1834860.1"
                     /translation="MNAVEPIRDKDDIQAMKDYLRSWNERNYILFLVGINTGFRIGDI
                     LKLRVKDVQGWHIRVREQKTGKLKTIKMTRTLKNELRKYVEGKPLQHYLFQSRNGVNK
                     PLDRRTVYWILKTAGNDLGIENIGTHTMRKTFGYHYYKKYKNVADLMIIFNHSSPAIT
                     LIYIGVRQDDLDSKMANFDI"
     gene            17487..17807
                     /locus_tag="VCA20_001017"
                     /pseudo
     CDS             17487..17807
                     /locus_tag="VCA20_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017795000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            17903..>18110
                     /locus_tag="VCA20_001018"
     CDS             17903..>18110
                     /locus_tag="VCA20_001018"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEP1834861.1"
                     /translation="MSKGRTVYKQFDANLAQKITAIFHDTQKGYRFISYQLRRHYQLS
                     PNPKTVLRYMRILGHQSPIRKKRYQ"
BASE COUNT         6102 a         3396 c         3668 g         4944 t
ORIGIN      
        1 ccgtcctgaa gaaagctgtg ctagataaga ttcttgctaa ctgtaaggaa cacaatatcc
       61 tacctagcta cgctattacc gttctacact ttgaggggct ttggggtaca tccgctgtag
      121 gtaaggttga taacaactgg ggcggtatga cctggacagg tcaaggcaac cgtccaagcg
      181 gtgttacggt cacacaaggc tctgcccgtc catcaaacga aggtggtcac tatatgcact
      241 atgcctctgt agatgacttt cttacagatt ggttctacct gctacgttca agcggttctt
      301 acaaggtcag cggagccaag acattctctg aagcagtcaa gggcatgttc aaggtcggcg
      361 gtgcagtcta tgactacgct gctacaggct atgataatta cctggtaggg atgtcaagcc
      421 gtctgaaagc tattgagtcg gaaaatggca gtctgacacg gtttgatgcc acatcaaata
      481 atgtccattc ggttgaccct gataaaatct ctgttgatat tgacggcatt gaagttacga
      541 tcaatggtgt agtctataag ctagaaaaga aaccagtcta atacacaaac aaagccctca
      601 gcaatgctgg gggctatttt ctattgtggc ggacatttcc aaaaatgtct gttatgatgg
      661 aaaaattttt tgaaaaaata tttgttaaaa acaaatgttt tcttgttgac aagtgttgaa
      721 aacaagtgta taatataatt aaagataagg aaaggagata agccaatgac agagcgagag
      781 cttaagaaga ttgctaagaa gcaaggtttc agtaaaacaa actttggcaa agggtctcac
      841 gaggtttgga aacatccaga cggacggaca gtgacgatac ctaaacccaa agagaaggat
      901 tacagacctg gtacactgaa caatattctc aaagtcttgt atggggagtg agggcactcc
      961 tccctgtacc cctaaagggg gtactctgat cattggctta atctatcact atgaaatata
     1021 attatttagc attgtttgaa gcagataagg aaaatggtgg ctacagcatt tctttccctg
     1081 atttccctgg ggcatttagc gaagctgaca atctaagcga agctattttc aacgctcgtg
     1141 aagttcttga aatctatacc atcatgtttg aagacgaagg taaagaattt cctaaaccat
     1201 catcattcaa agcacttgca agcaatctag caagcgatga tgatgtgatt caggctatct
     1261 ctgttgatac cgagcttgtc cgtgagcgtg aacgctctaa aattgtcaat aagaccgtca
     1321 cactgccaag ttggcttgtc gaagtcggca aagaaaacaa agtaaatttt agccagctat
     1381 tacaaaaagc aatccgtgag gaattgcagg tataagaaat agcggccaaa cttggtcgct
     1441 aatttctgca taaaaaatac tgatataaga ataaatattt tataataaaa ttgaaaccgg
     1501 aaaaactgtc tgccccaaat ccgccccaaa agttttaaaa aatgagtata gataggtcaa
     1561 aaatataaca aaaatagcta aaatcagcgt attttttaaa acatacaaca acgtgttttg
     1621 ctataaaatt cctgcagggg agatagagag tatacaaaaa aagcctaaat gatgggcttt
     1681 tttgcattat tgaatttcct caactgctgt ccataaatag tctgtttttt gtttataaga
     1741 taagctatgg caagtacagg aatagcatag ggtaggatat tagcaccgaa tacttcataa
     1801 ctaatgaaga gaggagctag taaggtattg gtggctgctc caaatacggc acaataccca
     1861 agggctacaa caagttcagt tggtagacct agtaatgctg ctaaaacagc cccagaactt
     1921 gctccaatcg caaagagcgg agtaacttcg cctccttgaa aacctgcagc caaacaaaga
     1981 caggtgaata ggagttttag tagccaatcg taagcaaaca cctgttcccc agctaaactg
     2041 gcatcgatca aattggttcc tagaccagca taacgacctt gatggaagaa aaatagtaga
     2101 acggtcagtc caactcccat gatagctatt ttaatgtaag gatttggaag ccatcgagtt
     2161 gagatgcttt ttgcttgcgc cagaaaccaa gcaaataaat tgccgatgaa accgaagcag
     2221 atggcaatca gaagccattt aaaactatct ataaaatgga atgaggtagt agtgatggca
     2281 tgcgaaaatt tctccaatcc cagtaaatgg gaagtagtag aagccgtgaa agcggcaact
     2341 agacaatagg ggagactagt ccaagaatat tggccaacga ttaaaacctc aatggcaaag
     2401 aaactagcag ccaatggtgt ttgaaatagc cctgcaaaac ctgcggccat accaatcttc
     2461 gttatcatat ctttgggcaa gtgtgtgaaa ccatgttttt gtaaccagtg tgagagactg
     2521 gctccaagtt ggacagctac accttcgcga ccaacggaaa caccgacaag atgactgagc
     2581 caagttgtag agatgatgag cggaatgaga actggagaaa tttgcgcttt ctccccttgt
     2641 ccagctttga agactaagcc catacctgct tggacttccc tcccccattt ttgataaatg
     2701 aaaacaatca gcaaccctgc aagtggtaga aaggggatta gaaaagtaaa atattcagcc
     2761 cgtaagtctc caatccctag tagaattttc ccaaaaagtg tggtgattat tcctgcaccg
     2821 actccaatta agctggaaaa gaaaaggagt tggatggctt gtttcatctt attgctcatg
     2881 attcgctaac ttggaagatt gataggttct tgattgggcc ataatatcgc taaaggttgc
     2941 ttgaatggca gagcggtaat catccttttg aactaatttt ccaactcttt caaggacagc
     3001 attttctcgg ctggtatcca aaacaggctt acccgttgct cgtttataga ctgtaacttg
     3061 gtcgaccaac tccattcgtt tttcaagtag ttcaaccaat tggcaatcaa tcgtattaat
     3121 ctgttcgcgg atgcaatcca aattcatctt ataaacctcc gtatattgtt tcagttgttt
     3181 tttagtatag caaaaaacaa ggcagaatac tagccttgtt tgaggaagtt accaacttgc
     3241 ttttttaacc ccaggaagtt gaccaagatg ggctaattca cggaaggtaa tccgactcac
     3301 accgaatttt cgcatataag cgtgcggacg gccatcggtc ctgtcgcggt atttcagtcg
     3361 atttggattt gagtcgcgag gcaattttcg aagtgcttga tagtcacctt tttctttgag
     3421 acttttacgt aaatccgcat agcgctcgat taactccaac tgtctctgat agcgtgccat
     3481 ttttgatttt ttagccataa aattctcctt tttaaaaact taaccagtta attatatatc
     3541 aaaactatta agattgcaag caataggtgt acaatcttaa taaaaggcta aaagaaaaca
     3601 gactagacct attgataagc cagtctgtct gtcagttaat tcattgtatc tagtttagta
     3661 acaaggtctg tcgcgaattg ctcaaggtta acaatatcct catcttcagc agcaaggtct
     3721 accttaacat tttcggcacc cttagttgca ccacgtgaac ccaacatgac atcaaagtca
     3781 tctactgcac tacagaagtc atcatagaag gtatcgccag aaccacaaac accgtagacc
     3841 ttaccggtca aatctaagtc agctaggtca gcatagaaat caacaatttc atctggaagc
     3901 tcaccgtctc caccgtaaga ataagtgtag ctggctacca cgatgaggtc cgcatccaag
     3961 atttcttcgg tttcaatagt cgtacattcg tgaacctgta cttccaaacc taactcttct
     4021 agcttactgg cgacgatgtc agcaatttct tctgtgttac cagtcatact agcgtaaact
     4081 atttttgcta gtgccataat ttcctccaaa ttacatagta actccattat accacactcc
     4141 catcattttc aaaaggggaa gtttatgcta aaatgatgaa aaggagttgc tatgacaatt
     4201 tacagtaaaa ttattgaaaa aattcacgaa tacgaaacga ttattattca ccgacataaa
     4261 cgaccagatc cggatgccat tggtagtcaa gttggcttgc aaaaactctt gcagaagaat
     4321 tttcctgaaa aaaccatcaa ggtaaccggt tttgaggaac caaatcttgc ttggatggca
     4381 ggaatggatg tagttgcaga ccaagattat caaggagcct tggttattgt gacagacact
     4441 gccaatacgg cgcgtgtgga tgacgaccgc tatgctcaag gagatttctt gattaaaatc
     4501 gatcatcacc ccaatgaaga gccttatggt gacctgctct gggtcaatac ggaagccagc
     4561 tcatgcagtg aaatgattac ggaactagcc atccaaagcc agttggaatt ggatggggaa
     4621 actgctcgtc tgctatatgc tggtattgtt ggcgatacgg gacgttttct ctatccagca
     4681 accagctcgc gcacctttga gattgttgct tgtctccgcc aagaagattt tgactttgca
     4741 aggatgtctc gccagatgga taccattgat ttcaagattg ccaagttgac aggttacgtc
     4801 tacgacaact tggaagtgga tgaacatggt gcggcacgtg ttattttgac acaggatatc
     4861 ctcaaaaaat acggggtgac cgatgcggat acttccttta tcgtcggggc tccagggcgg
     4921 attggcactg tacaatcttg ggggattttt gtggaacaaa ctgacggaaa ttacagagtc
     4981 cgtttgcgca gtaagtatct tccaatcaac gagattgcca aacgccatga tggcggtggc
     5041 catccactag caagtggtgc taactcttat tcactctccg aaaatgagca aatataccaa
     5101 gaaatccaag aggtagtgag gaatgcaagc aagtagtcgc tcccttcggc ttatgggcac
     5161 catcattgag accagaatat ggcatcctca ggcagaaccg attttggatc aggtagaaga
     5221 gcttctctat ctctacaagg atcggttttc tgccaatgac ttgacctctg aattgatgga
     5281 ggtcaacctc aatgcgggtg ttcaggcggt tcctgttgcc gatgacctgt atgaattgat
     5341 taaattgggc aaagaacaca gtttggctca gggatctttt ttgaatatta ccatcggtcc
     5401 ctttgtgcaa agctggcgga ttggctttag cgatgccaaa cttccaacca aagaaatgat
     5461 cagtgaaaaa ctccagctca tcaatccaag ggacatcgaa ttagatgatg aggagcagac
     5521 agtctatttg aaaaaagaag gtatggcgat tgatctgggg gctctggcaa aagggtatgt
     5581 ggcagataag attgttgact tcttgaaaag aataggtgtg gcagctgggc tgattaatct
     5641 gggtggcaac gtcctgacct ttggtcaagc tcctcacaat gcagatggct attggcgaat
     5701 tggtatacaa gaacctcaga aaccacgtgg tgaaaatgcc ctcgtcctaa aaataggaga
     5761 agaatcagta gtcacctctg gtatttacga aagaaccctt accgtgaatg gaaagaccta
     5821 tcatcatatt ttgagtcccg agaccggcta tccaatggag tcgcagctgg ctagtttgac
     5881 cattgtgtcc aaacaatctg ttgacggtga gatttggacg actcgactgt ttggtcaaga
     5941 aataactcgg atttataaga tggtagaaga aactgatggt atcgaagcag ttctgattgg
     6001 gctggatggc aggattctag tgacctcagg gctttctgag aaagttcttg ctggtaaaga
     6061 aagaatttct gatagcttgg gcagtccttc aagaggggga tttggcgcca gagtaacttc
     6121 tgaccttgct tcaggtgctt ccgtactata acttgaaaaa atattaaaaa aataggtaaa
     6181 aagacttgcc ataagaacaa acttttggta aacttaaatg gttataatct atggctcgga
     6241 aagagtccat ggcagaaagg aattttaaaa tgaaaaaaga tatccatcca gaatatcgta
     6301 ctgttgtctt catggacaca actactggct acaagttcct tagcggttca actaagaaat
     6361 ctaacgaaac agttgaattc gaaggtgaaa cttacccatt gatccgtgtg gaaatttcat
     6421 cagactcaca cccattctat actggacgtc aaaagttcac tcaagcagat ggacgtgtgg
     6481 atcgcttcaa caaaaaatac ggtctcaaat aatcagcgtt ataaaaccgt tagaaacgtt
     6541 gattttaagc cattcccgtg ccttgggaat ggttttcttt ttgaaatggt aacggaaatg
     6601 gtaacggttg ggcgttacat tcccaaaaat tgggcaaact tttcagccgt tttttctttt
     6661 gtattttccg taacgtgagc gtaaatattc atagttgttt taatatcttc atgccctagt
     6721 ctttcctgta cttctttaat cgatgcacca gcttcaaaaa gtaagctaca atgagtatgg
     6781 cggaagccgt ggggcgtgat ataaggcaag tcatattttt tcagaataca acgtagatta
     6841 tagtttatat aatcaaaact atgggcgttg cctgtttctg ctggaaaaac taggtcagtt
     6901 ggtttcacgc gctgggggaa gtttaaaaat tgtctagtct tttcccactt tttcagaata
     6961 gacacggtaa tagggtcaag cgtgatagtg cgcttactct tttttgtttt gggtgtttga
     7021 tagacgatac tgttattttc tccgtaggct actatttggc taactgataa acgtgatgca
     7081 aagaagtcta agtctttcca ccgtagggca agaacttcgc tttttctcat acctgtaaag
     7141 gcgagtaagc ggaaaataca ggctatttct tccttgtctt cctttgctac gcagtctagg
     7201 aacttttgca agtcttcacg gctgtagaag ttctcttttt tcttttcttc ttcatcgact
     7261 tcagaggatt tacggggctt tattatcttc ttcatggggt tatcatgaat atagcccaga
     7321 tttaccgcaa aatctaaaac catcttagtc atcccaataa agttagcata tttcttgtag
     7381 cccttaaacc attcttgtgc ctgtttttgg cacattggta ggcttatttt atccacgtag
     7441 cacgcgccaa atgctggaat gatgtgtagg tctgcttgtg ccttttgtct ggcgtatgta
     7501 ctagccttca cggtgttttt atagtgttct agccatatct tatacacttc aataaaggtt
     7561 attctatctg gcttgtcaaa aaatccgtct tgctccactt ttagaagtag ctggctttca
     7621 gctattcggg cttctttttg agttttaaaa ccgctctttt ttacgcgctt ttctttgcct
     7681 gtaagtgggt ctatacctac ataggcgata taagagtatc taacagagcc gtcttttagt
     7741 tggtattgtt taatcatttg tttatccttc ctattggttt ccccacctaa tcgaaagaat
     7801 aaaatagtat gcgcgtgttt ataagtggtt tcttatagtg ttactaagaa tgaaaaaaag
     7861 tgcctagcaa aactagacac ctcacacaaa tatacaccct cgggcgttat aaatgctttc
     7921 tccgtttatc tagtttatag tatttgggat taaatatcaa ttataaaaat atggttgctt
     7981 acatgttgcc atgtgtaagc ggggagaatt tgacaacaaa ataaattatc tgataagata
     8041 tacaagcccc ctttgaattt actgaaggga ggtggtttag ttgctagagc ttatcctcac
     8101 aagtattcta gcgccgttat tggttaatat cttgactgaa gtagttaagt attatttaga
     8161 cgataacgac gactaacccc aaacaggggc aaacaaaaac cctcaacgct ggcacgttgg
     8221 gggctttttg atttagtgct agaattgtcc tcacaacaat tctttacact atgattatac
     8281 ccccattata ggttatatgt caatcaattt gtatattttc acgcgccgac ctgctggggt
     8341 tggcgttttt aagtaatttt gtaatatttt tgtctctata aatggaagtg ccttaatcaa
     8401 tcgtctactt gattaaaaac aaaggtcaat tatcatattt tgtttttatt actcctaaaa
     8461 cctcatttaa aaatatcatt gcttcatctc cgcgttgttt ttcttccgat acataactta
     8521 atatgttatc aactatttct aattcttttt cagttaattt tttatcagta tctttttgta
     8581 tataagctcc aacaatttga tttgctattg tttcagcttg ctccctgtgg tgtcttaaca
     8641 atgcaaaata aagaaaaata tccaaatttg aaaaaaattc tttcccgttt tcggttaatt
     8701 ctccatcacc aaaaaaatca gtttcatcta ttttttcaat taaatcattt tcttccctaa
     8761 aatcactata ccccaacaaa taccctacac ttacgccgaa atgtttagcg agttttttag
     8821 catttttttc cctaattgaa ctttctccat tttcccattt ttgaagagtt cgtaagttaa
     8881 cgccaatttt tttggcaagt tcatcttgag ttaaattatt ttcttttctc aaaattttta
     8941 gcttattcat atgttttaac tacctttcaa ggcacattat aacataaaaa caaaaaatat
     9001 gaatttaaaa gccgtttttt tcttgacaac gccttttaaa tgcgttaaaa tcacaaatat
     9061 agcaaagaaa cggcttttaa agccgtgtga aaggaggtgt ataaatgcta ctaacggaag
     9121 accatgctag aaaagtacgg cgcaagcgtg cagacttaat gctgacgaaa aaggcaactg
     9181 ctgaaaagct aggtattaag ccccaagcct tgactaaagt tgagcagggc aactatgacg
     9241 ccccgcgccg tatctatgaa agtgtcataa attggctact agaagatgtg taattttcct
     9301 aactaccttt cacacacact acaggaaaac gacacagaca aagaaagggg gtaaaccata
     9361 ggcggactac tttatctaac cctaaaaaag acagctaggc acattctagc tatcttacaa
     9421 tggctattta ctcatgattg accaccgacc aagggcaatc acgcgcagaa aaaagaacta
     9481 aaaaccattt taagggcgta cccttaaaac tacgaccaac gacaaaaaca aatgattaaa
     9541 cgataataca ggtttcccca cctaatccaa agaataaaca cgcaggcgcg tgctagaaag
     9601 gaaatacaac atgaccacac ttgaagaacg ctattgtatt gagcgtagag ccttcttagg
     9661 acaacgggcg acacgcctag aactcctaga aagggttatt taccttgaac taatgctaat
     9721 ggatatggca gatagcacac tagatacaga cttcccagaa atggcagtca ctacaacact
     9781 agaccatatc gcagattttg gcgatgaagc cctttactta ctagagagcc tagaccatga
     9841 cggctattat aaagcccttt gggagcatac aaaagacaca ggggcaaaca atggcaagta
     9901 aacaacttga agcactccta gagcgtgcta acaagtcaga tgaagaacta gactatatta
     9961 ccgattatct agctagtttg aataatgaag ccatagaaac gacactagca ggcaaatttg
    10021 aagccgttag ccgtttcata tgggagatac aggactattt acaagaaaaa ctaaaggaga
    10081 aaacacaatg actatgattg acaaaaatac agtacttttc acttatggag aggataacga
    10141 gcataaacaa acagttggca catttttaga actagtaaaa aatgaggacg tagaaaaact
    10201 ttatagtatt ggcgaagcct tagaatcaga aggaaaaagt ctaggtaaag aattaaatga
    10261 aattgctgga acatttgaaa acttaatcga tagtttactt acaacattag aaggagaaaa
    10321 cacaaaatga gcaagaaaca gacctataaa gagtttaaaa aatgggtaga tgaccatgta
    10381 aacatggcgg aagctatggg actagatgaa aatgctgaag ttttacacga tatggtggaa
    10441 cataacgccc aagcatgggt acaactccaa gaaatgctag aggaacacac tctacattat
    10501 caaccaagag ggaagagaaa acgaggtaaa caacatggag acaaataaca ttgtaaccat
    10561 taatagcgcg gaacttattc accttactac ctacgctttc tatatgaaac atatttcaaa
    10621 aagcctacaa aactttacaa aggaactaga aaaaacgctt gaacctaata atgtagccta
    10681 tttaacactt gcttgggcta aaatcaatga agaaagcgag cgagcaatag aaatcctaga
    10741 accaaaaacc agcaaagaag actataaagc ggttatggaa gaacttgaaa cgttaaaaaa
    10801 tacacagtac accacagaag atatagttct aatgacgtgg gaaggatttt agagggggtt
    10861 gaaatgacta aaacaccatt tacacaagaa ctactacaac aaatttatga agataatggg
    10921 ctagttagct ttgatttact cctagaacgg ctgaaaggtt ggacgattga ggggattaag
    10981 gcacgtttta accaatggcg acaccgtggc attatctctt atagcttgct gaatgatgaa
    11041 attgacgaat ttcaattttt aaaaaccaaa cgagaagaaa aacaagaaat taccgagggg
    11101 cggaagttaa agctagacga gtatttcaag caagttttgg caacggctga cattatcaat
    11161 aagccaacgg caagcgatac aaaccgccta aaggctattc agctccaaca acaagcccta
    11221 acggaaatac ccgacgacat ttacaaagaa ttttctgagg tttacgccta gagggggtta
    11281 aattctcaaa aaaaggtaca ttctagcgat tttttagaat gggtagtata aagataccaa
    11341 aaaacaaaaa aacgccgtat agacgatttt aggcggtata ttgaacagga aggaaaaaac
    11401 aaatggcaga agcattgtta agcgaaagcg caagccttga acttgtccgc ggtgcgctag
    11461 ttttggcgcg tgaaatggca aaggacgaaa tagaacggct aaacaaaaag cccctacgac
    11521 aaggggaagt aatggagtta tacggctttg accacaaata catgaaatat ctaaagtcat
    11581 gcgggttaag gtcacgccgt caagggaagt ctatctacta tgatgtgcga gatgttgacg
    11641 aaatactgga aaagttgaaa gaatgacaac aaaaaaggct tataccagcg accaaactga
    11701 caagccttca aatgattaaa caataaccaa ggaagggcga acccttgtaa gcctttgtta
    11761 ttgccttaat cataccataa gcacaataag aaggcaagtt aatattattt atttcaagat
    11821 tttaagattt ttaaaatcaa agacagaaca cactagctag gctattattt tggcacagac
    11881 ttactaagta agtttggagc gggcaaccgc tggggatagt ctaggctagt tccaaaacat
    11941 gtatagtaaa agaaaaattg tagacaggtc aacacacgga aaaagcacgc gccaccgcgc
    12001 gagccaatac cagaaataca agaataaaat gtggaagggg gtcaaaatgg acttaaaagg
    12061 aaacgacaag cgtatatata gcctaattgg tgtagggatt gaaaaagcca tcacggcacg
    12121 ccacatagca caacaaacca acctagataa aagaacggtt agggaatgtg ttagacggct
    12181 gataatcaag cataaaatac caattattgg caaccgcaaa ggcaatcata agggctattt
    12241 tataccagcc aatcatagtg aactaatggc aggtattgga gcgcttgaaa aacaaatcga
    12301 ggaagaaaag aaacggcttg aagtattgct agaagccgag gtataaggaa aatcatacat
    12361 gaaacagtat tttaaacaat ttgaagaacg gttacaagtt gcagaagaaa agctagacat
    12421 tttaagcgat tggcatattg ccaaaggtca caaggaagct actaaaattg ctgaagagtg
    12481 taggacagct attacaacac tctggatgga gttttacagg ctttcagagg cttataatga
    12541 ggcttataat gaggcttata atgaggctga ggcaggccat gaagaatttt atcaagcgaa
    12601 tgtggattat ttattagggg aattaagaaa acacgataat atgcttttag aaaatgcgat
    12661 atcaatcggg aaagggcgcc ccaactatct tttatttgat tatctggata gagagcaacg
    12721 gatttttgaa aatcctgaca atttagtacc agcgccaata gggaatattt ggcactatat
    12781 ccgcagtcta attatcaaag accaaaaaga acgaggtatt ttataaaatg caagaaatga
    12841 caatagaaca agcgttaaca ctcatagcaa tatttacacc gctaaacctc tttctatggt
    12901 ttagggtagg tttgggcacg tgtcagctcc ataacaagcc taaaaaagtt tctgagggta
    12961 aacataccac gcaacatata aacgataact acggggctta tatttggttt gctggcaaga
    13021 agtataacta ggagtttcaa aaatgaatat taaagaacaa ctaaaaatta ttgaaagaga
    13081 agaaccagaa ctatttgatg tgattttcgc ttgtataact ggaaaaatac caaaagagga
    13141 agtagattat tttttatctt tgtcagatga gaaagatagg gctgaatggg taaagcaaca
    13201 aatagtgaaa cgaaagaaca tagaaaggga aaaagatgta tagcattgtg gaagtgacta
    13261 taaaacgtgg ggaacaaata ctactagatt tactaaaaga taatccgaca cgcgcaataa
    13321 tgcaaaatga ttttttccgc tttttctact acttgccatt tggtggttat atcacacctt
    13381 tcagtctaaa caacattact ttgaaagtat ttgaaaaagt accagccccg aaaggatggg
    13441 aacttattag agattatcca ataagtatca tgaaaacaag tctattagca aagctagaag
    13501 acattgagaa atacacgtta gaccagcaaa gacaggcaag cggactatat ctagaaggct
    13561 gggtgtttga tactatcaca aatggaattt acacaaagga agaaacggct atttttatta
    13621 gacaaatgta tttacatggc tatgattttg aacaagtaat aagcttattt actgccattg
    13681 tgaaacggtc aagcctagca agttatttct tagacatgat gaaacggctt tattttgctg
    13741 aagctacagg aaatacaata gcatacatta tcaatcagca aaaacaagat acaaaaatca
    13801 taagctagac catagaaaga aggtgggtag tattacacag tatgaaagac aaataactag
    13861 agaaacgcca tataatgcgc gtgatatacg gcttagaacg tttaaaggtt ggcaagggaa
    13921 agtatttcaa tttatccaac acaagcagga agaacgctat agaaagctat tagaagacgg
    13981 gttagacgat aagcaggcac aagcagaaat taaaaagcct gttccacctt tgatagttgc
    14041 ccaatatatg gcggaattgc tgacagtttg cagaattgaa agcgatgagg gaacaaaacc
    14101 cgtttatttt tataaccctg atgaggggct ttatatcaat gatagggaat ttttaaaaga
    14161 ctttataagt attatagagc cacggcacaa cgaaaaaagg gctagcgatt gtatttacca
    14221 cttaacccga aaagcaccgc atagacaggc ggaaaacgcc ccacatttga taatagttgg
    14281 caatggtatc tacaaccgaa agacacggac actagaacca tttacagaaa caagggtatt
    14341 cactcataag atacaaacca actacaatcc acacgcccaa agcccgacta taaagggctg
    14401 ggagtttaag gactggctat tagatttatt taacggagac aaagaacttt atcagctatc
    14461 ccttcagcta ctcaatgccg ttgtacgtgg ggaaagttac gccaaaatgt tttggtttgt
    14521 aggagaaggc ggaacaggga aaggaacgct acaagagtta ttcatcaacc taataggacg
    14581 gcagaacata gcaagtataa agataacgga cttagatgtt aataatcgct ttaccctagc
    14641 ccaagcaatc gggaaacaag ctataatagg cgatgatgtg caagctgggg cggttattcg
    14701 tgatacttct aaactattca gcctagttgg tggggatact gtaacggtag agaaaaaagg
    14761 gaaagatgct tatagtacct ttataaaaac tgttgtcatt cagtcaacaa acaccctacc
    14821 aaagatacgg ggagactatc acgctatacg gcgtagaatg gttattttgc cctttaataa
    14881 gcactttaag gggaagccta accgagcaat taaaaacgat tatatcacgc gccctagcgt
    14941 cctagaatac gtgttaaaaa cagttatcga cctagatttt aaggacttta tagagcctag
    15001 caagtcaata gatttactag acgaatacca ggaagctata gaccctgttt tagcattttc
    15061 acaaaatcta tttactaatt tacattctac ctttattcct aatgattggg tatggtggta
    15121 ctttacaggc tttgcagatt atcataattt tcaacataat ttcagtagcc aaggttttca
    15181 tactcacttt ccgcaatatt tacccgaaga ctgggaaaag ttggcttatc ctattagtct
    15241 accaagaggg gcagaccttc ccaaaggttt ctaccccaag gcagaccagc cccactattt
    15301 acccctttat gcaccaatca aaggtaaggc gcagaggggc tataagaaaa aataagttac
    15361 aacacttaca acacttggca caacactttt tagaaaaagt gtagtgacga aatcccaaca
    15421 gccccaacgc tttacagaat attactacac ttactacact tattatatat aaagtaataa
    15481 aaagaaatat acttacttgt gtatgtatat atattataaa aaaatcaacg tattcaaaaa
    15541 aagtgtagta agtgtagtaa catctccaaa ccccttgcac cccaagggtt tccgctacaa
    15601 cgctttatta ttctaagtgt tgttaagcgt tgtaaggtgt tgtaatttta atacagaaag
    15661 aaggtggaat tattgaagca aacgaatgta gagaacttca gaacgcgaac cgatggaaag
    15721 tataagggaa tgatggaagg gcacgcccaa tggttagaaa acttagagcg tcactatttc
    15781 ccgaataagt ccgctagtga atatgacaat ctgaatgcgc gtgaggaaga agggaaagaa
    15841 gctaatgaat gcagttgagc caatacgcga caaagacgat attcaagcca tgaaggacta
    15901 tctaaggtca tggaatgaaa ggaactatat tctatttctt gtgggaataa acacaggttt
    15961 tagaattggg gatattttaa aattaagagt taaggatgtc caaggctggc acattagagt
    16021 acgggaacaa aaaacaggta aactgaaaac tataaaaatg acccgaaccc taaagaatga
    16081 gttaaggaag tatgtagaag gcaaaccgct ccaacattat ctttttcaaa gccgtaacgg
    16141 tgtaaacaaa ccgcttgacc gccgtacagt ttactggata ttaaagaccg ctgggaatga
    16201 tttaggtata gaaaatattg gtactcatac catgcggaaa acattcggct atcactatta
    16261 taaaaagtat aagaatgtag cagatttgat gattatattc aatcattcaa gcccagcaat
    16321 cacgctaata tacataggag ttagacaaga tgaccttgat agtaagatgg ctaactttga
    16381 catttaaaga caaatatcaa gtattaatta gacataatga agaaaatgct agtaattatt
    16441 taaggaatag ctgaaagcct tgatagtatt gagtttagag agtgaaacat aattagacag
    16501 aatataagat atgactaatt caacgaacac gaaaaccact caaaaaatac aaaaagaata
    16561 taaaaccatt aagcccttta catgttagta ttaagggctt atctattgtc tgtagtgcat
    16621 ttggaagata ggggggtgta ccctaccacc ctatcgcctt tggacttcac gccgtcattc
    16681 tacatttttt ctcgcgtgaa aaatattatg aacaactatt acactgatga tagtagtcta
    16741 gtataaataa gccccctgcc taaacaaacc gcttttctgc tggggaaacg tgcaacggga
    16801 aggggtacct cggaaaaaat atctagtcta tatttatcgt aagtcaaaaa caaattagat
    16861 tataatagat tgttgatttt atattttacc cccctacccc gttgaaatat tttcaaatga
    16921 ttgggaaccg agtaagggaa cttcttccaa gtcgggaacc gttagacaaa aagggggtaa
    16981 aaattttgta gaatttatga caagaaattt tttctatatc ccccctgatt tagaatgtta
    17041 gcacatgcag aaaataacgg tgtcaatcct cttgtgtatc tctccccaaa aattgaccaa
    17101 aattgataca taaaaaataa taaaaatacc aatagtgtaa aatttgaaat aaacattaaa
    17161 aagctgggaa taccaacttt ttgcatatta tttcataaat gtgtataaaa taacttactc
    17221 tttattcctt ggaataggtg gggaaattct ttattttggt aacggaaaaa gttccgattg
    17281 atgataattg ataagaatat ataagaattt tataaaatct ttcttctatt tatatagtta
    17341 tcttattatt taatgaaaaa tgataataaa taaaagggta taaaatacgg tctcaaataa
    17401 aaaaaacagt ccttaatgaa aagaaccgct tataatggac agtataaaaa agttatatac
    17461 tgttcatata aggaggttct tttttcatgg ctaaaaaagg aagcaagttc agcaaatatc
    17521 cacccgagtt caaaccacag gtagttgaag actacctcag cggaaagagc ggagggatgc
    17581 cttctattgt caaaaaatag gggttaaaat cagacaacca agttttaact tggacaagaa
    17641 aataccgaga agaccctact ttactctccc aagatttacg aggtagaaag tccactggtc
    17701 gtcccaaaac aaggaatcta gaagagatga cccttgagga acagaatact tatctacgaa
    17761 tggagaatgc aattttaaag accttaaggg ctcccctcaa tccgtgagaa gagtccaagt
    17821 cgtggagcag ttgaaagatc gttactctgt ctcctcttta tgcgtctatt tcggcttccc
    17881 caggtcttct tactaccttt ggttgagcaa agggagaacg gtctataaac agtttgatgc
    17941 gaacttagcc caaaagatta cagctatctt ccatgacact caaaaaggct atcgcttcat
    18001 ctcttatcaa ctgagaagac actatcaact ttctcctaac cctaagactg tcttgcgtta
    18061 tatgcgtatt cttggtcacc agtctccgat tcggaagaag cggtatcaat
//
LOCUS       DATUCG010000038        17957 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.039, whole genome shotgun sequence.
ACCESSION   DATUCG010000038 DATUCG010000000
VERSION     DATUCG010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17957
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.039"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(2..316)
                     /locus_tag="VCA20_001019"
     CDS             complement(2..316)
                     /locus_tag="VCA20_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HEP1834862.1"
                     /translation="MPLRHYQSHEYQYEEYIPKEKKANFQTYQAKNANKERLKELLFF
                     VNIAFFSLITVIASYVYLSVGIPVFIAIILASATGFLGLRLVQIGLKKKFKPKQVIRK
                     NE"
     gene            477..1406
                     /locus_tag="VCA20_001020"
     CDS             477..1406
                     /locus_tag="VCA20_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012997850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEP1834863.1"
                     /translation="MEKIIITATAESIEQIKELLAAGVDRIYVGEEDHALRIPTNFTY
                     DELRDIAELVHSAGKELTVAANALMHQDMMDNIKPFMELMREIKVDYLVVGDTGIFYI
                     NKRDGYNFKIIYDTSVFVTSSRQINFWKDHGAVEAVLAREIPSEELFDIAKNLEMPAE
                     ILVYGASVIHHSKRTLLQNYYNFTKADETDLSRGRGLFLAEPSDPDSHYSIFEDKHGT
                     HIFINNDIDMMTKLSELDEYGYHNWKLDGIYCPGQNFVEIVKLFVQARDLIEKGDFTY
                     DQAFLLDEQVRKLHPAERGLDTGFYEFDRNKVK"
     gene            1674..2960
                     /locus_tag="VCA20_001021"
     CDS             1674..2960
                     /locus_tag="VCA20_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEP1834864.1"
                     /translation="MSKTLKRPEVLSPAGTLEKLKVAIDYGADAVFVGGQAYGLRSRA
                     GNFTMDEMREGIEYAHARGAKVYVAANMVTHEGNEEGAGAWFRELRDMGLDAVIVSDP
                     ALIVICATEAPGLEIHLSTQASSTNYETFEFWKELGLTRVVLAREVTMEEVAEIRKRT
                     DVEIEAFVHGAMCISYSGRCVLSNHMSKRDANRGGCSQSCRWKYNLYDLPFGEERRSI
                     KGEIPEEFSMSAVDMCMIDHIPDMIENGVDSLKIEGRMKSVHYVSTVTNCYKAAVDAY
                     LESPEKFEAIKQDLIDEMWKVAQRELATGFYYSPPSENEQLFGARRKIPEYKFIAEVV
                     AFDKETMTATIRQRNVINEGDQVEFYGPGFRHFETTIQDLHDSNGEKIDRANKPMELL
                     TIKLDREIYPGDMIRACKSGLINLYQEDGQSLTIRA"
     gene            complement(3253..3576)
                     /locus_tag="VCA20_001022"
     CDS             complement(3253..3576)
                     /locus_tag="VCA20_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834865.1"
                     /translation="MKRFLEVIFEQFTVFLEHGKAPKSVEPDSKEDIKNNLELALYKK
                     AAVHVIYGDRSFTGDIVKYDTDRQRIIMKNFKRQVTSIIKIEDIKKVTLVPDSIKTSQ
                     QQTKG"
     gene            3668..3883
                     /locus_tag="VCA20_001023"
     CDS             3668..3883
                     /locus_tag="VCA20_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018167072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEP1834866.1"
                     /translation="MAEFIFEIEEKLLVLSENDKGWTKELNRVSFNGAPAKYDIRTWS
                     PDHTKMGKGITLSNEEFQVLLDAFANK"
     gene            complement(3895..4434)
                     /locus_tag="VCA20_001024"
     CDS             complement(3895..4434)
                     /locus_tag="VCA20_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEP1834867.1"
                     /translation="MKTTSIKSLVYIAIGTALISTLSQISLSIGPVPFILQTLAIGLV
                     ACLYKPKEAITSVSLYLVLGAIGLPVFAGFSGGFAALAGPTSGFLWGFLLYTIITSAL
                     TKSDSSPVTIFLACLLGTSICFLLGCFVFKLVSGVSWTDSIGWTVLPFILPDLAKITL
                     VMICHRLLQPVIKKEAYFA"
     gene            4639..5236
                     /locus_tag="VCA20_001025"
                     /pseudo
     CDS             4639..5236
                     /locus_tag="VCA20_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909475.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
     gene            complement(5431..6780)
                     /gene="gorA"
                     /locus_tag="VCA20_001026"
     CDS             complement(5431..6780)
                     /gene="gorA"
                     /locus_tag="VCA20_001026"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEP1834868.1"
                     /translation="MKVFDIIAIGGGSGGIATMNRAAIYGAKAAVIEGSYLGGTCVNL
                     GCVPKKIMWYGSQVAEAIHHYGPEYGFTSQEVTFDFATLRKNREAYIERSRASYGNTF
                     NNNGVKVFQGFAHFVNNQTVEVNGELIRAKHIVIATGAKPVVPAIPGSELGIVSDDVF
                     AWEELPSSVAVIGAGYIAVEMAGLLHGLGVQTDLFVRGNRPLRSFDTYIVDALMEEMQ
                     RTNLPLHTGKTPVSLEKTDDGQIQINFADGSNHIAQKVLWAIGRKPNIDKLNLEATSV
                     QLTSSGHISVNEYQETAVPGIYALGDVTGEKELTPVAIKAGRLLAERLFNNKPTAKMD
                     YTTIPTVVFSHPAIGTVGLTEDEAIAQYGQDNVKVYTSAFTSMYTALENNRQMAKLKL
                     VTVGENEKVVGLHGIGYGVDEMIQGFSVAIKMGATKEEFDAVVAIHPTGSEEFVTMR"
     gene            6993..8153
                     /locus_tag="VCA20_001027"
     CDS             6993..8153
                     /locus_tag="VCA20_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEP1834869.1"
                     /translation="MLKTKKAKIALFGGLGVVAVALVGGALIFSNMLTSSPSSEEVAM
                     SMTYTVAKEGSIASSTLLTGTITAADEQYVYYDPSKGDLSEVLVAPGTQVEVGTPLIR
                     YDATELQSALDTAVRGRDKIGRQIEDLRTNGQTVQTTGDAETDAAATATAQRSVDMQL
                     ADLNDSYADAQAAVDKAYTALTEATVTSTVAGTVVEVNKAVSKNSTSSQTVVHIVNQG
                     SLQVTGSLTEYDLANIAVDQEVKLTSKVYPDKTWTGKITYISNYPSSDQAGAAATTGT
                     GGSGAKYPFKAALTSELGELKQGFSVNIEVVNTSKNILIPVSAVVPEEDKNYVWTLVD
                     GKAKKVEVTLGNADALNQEVTAGVAVGDKVITIPTPDLEDGKEVEANEEPAY"
     gene            8134..8829
                     /locus_tag="VCA20_001028"
     CDS             8134..8829
                     /locus_tag="VCA20_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1834870.1"
                     /translation="MKNQLIRLTNINKSYKNGDQELRVLKDIDLEVEEGEFLAIMGPS
                     GSGKSTLMNIIGLLDRSSSGNYWLEGEEVSQLSEKKLAQVRNDQIGFVFQQFFLLSKL
                     NAIQNVELPLIYSGVPSSKRKELAKQYLEKVELAERMTHLPSELSGGQKQRVAIARAL
                     VNTPAIILADEPTGALDTKTGEQIMQLLTELNNEGKTIIMVTHEPEIAAYAKRKIVLR
                     DGVITEDSQREGV"
     gene            8830..10077
                     /locus_tag="VCA20_001029"
     CDS             8830..10077
                     /locus_tag="VCA20_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP1834871.1"
                     /translation="MENWKFALKSIMAHKMRSLLTMLGIIIGVAAVVIIVAMGTGMSK
                     SIEKSLAGDQNNVQVYFTAFSRSSTAFGPFGTVSTEEPILEEEPDLTDSTLKGLLEID
                     GVSSYYSSISNMAEVSSGNKKAENVTITGVSQNYFAVKEYEILAGRQFTANDYSRFSR
                     MIMLDTALAEKLFGSIEDSLNQTISVNSNAYLVIGVYKDPKAGTAIYGMNSGGNAVMT
                     NTQLAAETGMKENSQIYVHVEDVTRATEVGQAAADYLTKATGLKEARYEIYDMSAMLE
                     EFKSQMSGVTLFIGAVAGISLLVGGIGVMNIMLVSVTERTREIGLRKALGATRGNILM
                     QFLIEAMVLTTLGGAIGLAIAQTIVFLLNVSKAMGERITAEVSIPVVLGSLAFSAVVG
                     IVFGVLPANKASKLDPIEALRYE"
     gene            10201..11265
                     /locus_tag="VCA20_001030"
     CDS             10201..11265
                     /locus_tag="VCA20_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEP1834872.1"
                     /translation="MVELGISTFGETTPLEKTGETYSHDERIRQLVKEIELADAVGLD
                     VYGIGEHHREDFAVSAPEIVLAAGAVNTKHIKLTSAVSILSSMDPVRLYQQYTTIDAL
                     SNGRAEIMAGRGSFTESFPLFGYDLHDYEELFDEKLDMLLEIDKETNLKWDGHFTQSV
                     DNKPVYPRPVQEDFPIWVATGGNVESTIKIAKKGLPIVYAVIGAGAHRFKPLVDAYRK
                     VARNSGHDPKKTKVAAHSWGWIADDHDKAVEEYYHPTKVITDNIAKDRPHWSEMTKAQ
                     YLYSLTDEGATIVGDPKRVAEKIIKTIETLELDRFFLHLPIGSMPHEDVLRAIELYGK
                     EVAPIVRDYFAKKEENQSQD"
     gene            complement(11850..13340)
                     /gene="lysS"
                     /locus_tag="VCA20_001031"
     CDS             complement(11850..13340)
                     /gene="lysS"
                     /locus_tag="VCA20_001031"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEP1834873.1"
                     /translation="MSTEHFEELNDQQIVRREKMTALAEQGIDPFGKRFERSANSAEL
                     KAQYEDKSKEDLEELGQTAIIAGRIMTKRGKGKAGFAHIQDREGQIQIYVRKDDVGEE
                     NYEIFKKADLGDFIGVEGDVFKTNVGELSIHARKLTHLSKALRPLPEKFHGLTDIETR
                     YRKRYLDLITNRESFDRFVTRSKIISEIRRYLDGLGFLEVETPVLHNEAGGAAARPFI
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADYQDIMDLTEGIIQHTAKAVVGDGPVTYQGTEIAIHEPFKRIHMVDAIKEQTGVD
                     FWQEMSFEEAKALAAEHKVPVEKHHTEVGQIINSFFEEYVEATLIQPTFVYGHPVAVS
                     PLAKKNDEDPRFTDRFELFIMTKEYGNAFTELNDPIDQLERFEAQAKTKELGDDEATG
                     VDYDYIEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
     gene            complement(13443..14069)
                     /locus_tag="VCA20_001032"
                     /pseudo
     CDS             complement(13443..14069)
                     /locus_tag="VCA20_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730229.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
     gene            complement(14072..14554)
                     /locus_tag="VCA20_001033"
     CDS             complement(14072..14554)
                     /locus_tag="VCA20_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEP1834874.1"
                     /translation="MAKKVKIKKTLVDQILDKAKINHDSLVLNAFEGQLPPGIEEHEI
                     YKTLALTGDKTGPIIGIVPITEHLSEKKLAKISGNKKVSMIPQKDLETTTGYVHGANN
                     PVGIRQKHNFPIYIDQSALELGHLIVSAGEIGRSIRIDSQVLADFVKADFVDLIEGRD
                     "
     gene            complement(14554..15396)
                     /locus_tag="VCA20_001034"
     CDS             complement(14554..15396)
                     /locus_tag="VCA20_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEP1834875.1"
                     /translation="MASWISRIIKGMIIALGFILPGVSGGVLAAILGIYERLIGFLAN
                     IRKDFKENFLYFVPVGIGGILGIALFSFPVEFLLQHFQVPVLWAFAGAIVGTIPSLLK
                     ESTQKSKRDSIDLAWLIGTFIISGLLLYNLSDLVGTLPATFASFVLAGTLIALGVLVP
                     GLSPSNLLLILGIYTPMLNGFKSLDLFGTFLPIAIGGVLAMVAFSKAMDHALKVYHSR
                     VYHFIIGIVSSSTLLILIPQASNKESISYAGANFVTWIAAIVLFILGAWLGLWMSKLE
                     EKYK"
     gene            complement(15499..16047)
                     /gene="thiT"
                     /locus_tag="VCA20_001035"
     CDS             complement(15499..16047)
                     /gene="thiT"
                     /locus_tag="VCA20_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HEP1834876.1"
                     /translation="MPTSKLPILAEVTIFSAIALVFDKIPLFTMPQGGSVSLVMLPIL
                     LLALRHGLGVGVLTGGIVGTIQLFYGGYFLNVFQVFLDYALSYAGIGLAGLVAPTLSK
                     QKDLKNATLIITLASFLGGSIRLLATFLSGIIFYADYAPDGMPVWFYSFTYNISYILP
                     STAIASILLILLYKARPGFYNL"
     regulatory      complement(16106..16201)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(16225..17079)
                     /locus_tag="VCA20_001036"
     CDS             complement(16225..17079)
                     /locus_tag="VCA20_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HEP1834877.1"
                     /translation="MRKKLNPMIVVGFFLSFFALVFITGVTGNTVNKTEVANEPAVTQ
                     SNTSTSSKTTNSSSSYYIANALEMKPIVDISAWQRPSEIDYDTLSKNISGAIVRIQSG
                     SHTKNENTATDKNGLDKSFDTHIKEFQARNIPVAVYAYVTGNSIENMQEEARSFYKAA
                     NKYKPTFYWLDVEEYTMDDMNAGVEAFRKELETLGAKNIGIYVGTYFMEDHSINVDRF
                     DAIWIPTYGDDSGYYNAAPNTDLNYDLHQYTSRGYVNGFEHHLDLNIITTLKDPNEVY
                     QKLFTTPE"
     gene            17191..17694
                     /locus_tag="VCA20_001037"
     CDS             17191..17694
                     /locus_tag="VCA20_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834878.1"
                     /translation="MHDKSEKVRRVSQLVVLSSLTIVLRVVFGAFPNIKPLTAIFLIV
                     LSYFSLMESWILMILVMVGSGLLFGLGTVVLWQVLSFAFIQFIWWLAIGPFIKKENIP
                     LWLQSLLAGILVFLYGLTISFLSATQFGTSPIVFWLNGLVFDSLHAASTVLFYPIIDY
                     IFRRFYK"
     gene            17691..>17957
                     /locus_tag="VCA20_001038"
     CDS             17691..>17957
                     /locus_tag="VCA20_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834879.1"
                     /translation="MKKISIMLTVVFSLFLVACTNTTEKQSDSAIEVTLQITTKDKEN
                     RQEVTAEEGDSVMDVLKEHHDIVEDNGLITEIDGVSQDASTNTYW"
BASE COUNT         5321 a         3510 c         3949 g         5177 t
ORIGIN      
        1 attattcatt ttttctgata acttgtttgg gtttaaattt cttctttagt ccaatttgaa
       61 ccaaacgtag tccaagaaaa cccgtcgcac ttgccaatat gatggcaata aaaacaggaa
      121 tgcctacaga tagatagaca taactagcga tgactgttat caaactaaaa aatgcgatat
      181 taacaaaaaa caacaattcc tttagacgtt ctttattggc attttttgct tgataggttt
      241 gaaaattagc ttttttctct ttcgggatat actcttcgta ctggtactca tgggactgat
      301 agtgtcttaa tggcatatcg tctccttttc tgctctttaa agaataccat atcattgatg
      361 aattattgtg tcatttatat tacaataatg ttacaagaag gatattagaa aagtctgaat
      421 attaaaacac agctagtcac ttatcagata tttctctttt tgatataatt atccttatgg
      481 aaaaaattat cattacagca acagctgaaa gtattgagca gatcaaagaa ctgcttgcag
      541 caggagttga ccgtatttat gttggggaag aggaccatgc ccttcgcatc ccaacaaatt
      601 ttacctatga tgaactcagg gacatagcag agctagttca tagcgctggg aaggaattga
      661 cggtcgctgc caatgccctc atgcatcaag atatgatgga caatatcaaa ccgtttatgg
      721 agctaatgag ggaaatcaag gttgattatc tggttgtcgg agatactgga attttctata
      781 tcaataaacg tgacggctac aatttcaaga taatctatga cacatctgtc tttgtgactt
      841 ccagccgtca gattaacttt tggaaagatc atggagcagt agaggctgtt ttggcaagag
      901 agattccatc agaagaactc tttgatattg ctaagaattt ggaaatgcca gcggaaatct
      961 tggtctacgg cgcctctgtt attcaccatt ctaaacgaac cttgttacag aattactaca
     1021 attttacaaa ggctgatgag acagatttgt cacgtggacg tgggttgttc ttggcagagc
     1081 cgagtgatcc agatagtcac tattctatct ttgaggacaa gcacggtacc catatcttta
     1141 tcaacaatga cattgatatg atgaccaagc tatcagaatt agatgaatat ggttatcata
     1201 attggaaact tgatggtatc tattgccctg gtcagaactt tgttgaaatt gttaagcttt
     1261 ttgttcaagc aagagatttg attgaaaaag gcgacttcac ttatgatcag gccttccttt
     1321 tggatgaaca ggtacgcaag ctccatccag ctgaacgtgg cttggataca ggcttctacg
     1381 aatttgaccg caataaggtt aaataacaat atttttagca caatttattt actttaacct
     1441 gacagagtta gccctgtcct aggttgccga atgaaaaatt gtcgttttca ctcggaacat
     1501 gtcttaatta tagttaatct acattctatc ctggcagagt tagctctgtc acttactatg
     1561 taaggtcaga gcacgaaaac gcctttgggc gattttctaa actctgcact aggtttccga
     1621 gtgaaacaac atcgtttaca gtattctatc ctgcaaatag aaagaagtat attatgtcaa
     1681 aaacattaaa gcgtcccgag gtcttgtcac ctgctgggac tttggagaaa ttgaaggttg
     1741 ccattgatta tggtgctgat gcggtctttg ttggcggcca ggcctacggt ctgcgtagcc
     1801 gagctggtaa ctttaccatg gacgagatgc gggaagggat tgaatatgcc catgcccgtg
     1861 gtgccaaggt ctatgtagct gccaacatgg tcacccacga aggtaatgaa gaaggagcag
     1921 gagcctggtt ccgtgaattg cgtgacatgg gcttggatgc cgttatcgta tctgacccgg
     1981 ctctcatcgt tatctgtgct actgaagcgc caggccttga aatccacctg tctacccagg
     2041 cttcatcaac caactatgag accttcgagt tctggaagga attgggcttg actcgtgtcg
     2101 tcctggcccg cgaagtgact atggaagagg tggctgaaat ccgtaaacgg acagacgttg
     2161 aaatcgaagc ctttgtccac ggtgccatgt gtatctccta ctcaggtcgt tgcgttctct
     2221 ctaaccacat gagcaaacgc gatgccaacc gtggcggctg ctcccagtct tgccgttgga
     2281 agtacaacct ctacgacctc ccatttggtg aagaacgccg ttcgattaag ggggaaatcc
     2341 cagaggagtt ctccatgtct gcagtggata tgtgtatgat cgaccatatt ccagatatga
     2401 ttgaaaacgg ggtcgatagc ctgaaaatcg aaggccgtat gaaatctgtc cactacgtat
     2461 caacagtgac taactgctac aaggctgcgg tagatgccta tcttgaaagc ccagaaaagt
     2521 ttgaagcaat caagcaagac ctgattgatg agatgtggaa ggttgcccaa cgtgaattgg
     2581 cgacaggttt ctactatagc ccgccgagtg aaaatgagca gctctttgga gcccgccgta
     2641 agattccaga atacaaattc atcgccgaag tagtggcttt tgacaaggaa accatgactg
     2701 caaccattcg tcagcgtaat gttatcaacg aaggtgacca agtagagttt tacggaccag
     2761 gtttccgtca ttttgaaacg actattcagg acttgcatga ttcaaatgga gaaaagattg
     2821 accgcgccaa caagccgatg gaactcctga ccatcaaact agaccgtgaa atctatcctg
     2881 gtgacatgat tcgtgcctgc aaatcaggcc tcatcaacct ctaccaagaa gatgggcaat
     2941 ctctaactat tcgtgcataa acatatagac atttagtttt tcttttatta cttcgacgta
     3001 agaggaaact ccgtttcctt atttccaact tcaaacattc cactggattg tttgaactcg
     3061 ctttgccgta ctctgcaggg gtgacttgct ccgcaagtgc tatctccaac cttgcacagt
     3121 cactcgactg tacaagctgt ctgtagctat tgaagcgaac ttgcttcgcc ctatctccaa
     3181 cctccaaagg ttccccaaac ctttggagcg agtactaaaa gtaaactaaa agactataaa
     3241 aaggctgaaa tttcagcctt ttgtttgttg ttgagaagtc ttgatgctat ctggaaccaa
     3301 ggtgactttt ttgatgtcct cgatttttat aatgctggta acctgacgtt tgaaattttt
     3361 catgataatc cgttggcgat cggtatcata tttcacaatg tcacctgtga aactacgatc
     3421 accatagatg acatggactg cagctttttt atataaagcc agctccaagt tattcttgat
     3481 atcttccttg ctatctggct caacagactt aggagctttg ccatgttcta aaaagacggt
     3541 gaactgctcg aaaataactt ctaaaaacct cttcatggca taatcctttc aaacttgata
     3601 taattatatt atctaatttt tataaaaatg acaagctttt tcgaatagat aagtgaggag
     3661 gtaacaaatg gcagaattta tatttgaaat tgaagaaaaa ttattggtct tatctgaaaa
     3721 tgataaagga tggaccaagg agcttaatcg agtatctttt aatggagcac cagccaagta
     3781 tgacatccgt acctggagtc ctgaccatac caagatgggg aaaggaatca ctcttagtaa
     3841 tgaggaattt caagttctac tcgacgcgtt tgctaataaa taaatgaagg aagcctaagc
     3901 aaaataggct tcttttttga taacaggttg caataaacga tgacaaatca tgactagtgt
     3961 aattttagct aaatcaggaa gaatgaaagg cagaacagtc catccgatag agtcagtcca
     4021 actgacacct gaaactagct taaatacaaa acagccgaga aggaaacaga tacttgttcc
     4081 aagtaggcag gctagaaaaa ttgtgactgg tgaggagtct gatttagtga gggcagaagt
     4141 gatgatagtg tagagtagaa acccccatag aaagccagat gtcggtcctg cgagtgcagc
     4201 aaagccacca gaaaaaccag caaaaacagg taaaccgata gcgccaagaa ctagatataa
     4261 gctgacactt gtaattgcct ccttgggttt gtatagacaa gctactaagc cgatagctag
     4321 ggtttgtaat ataaatggta caggtccgat agacagactg atttgtgaaa gggtggaaat
     4381 taaggctgtt ccaatagcga tataaacaag tgatttgata gaagttgttt tcatttgtta
     4441 tcctcttaaa attttatggt taacaaaaat tatagtgaga ttggataaga aagtcaatgc
     4501 tctttcatcg gctgtaacaa ctctataggt gatttttata acagctaata agaaacttat
     4561 atagtaaaaa gcgataaatt atatgtaaaa caacctatac ctatgctaga ataataaaaa
     4621 aagaaagtga gaatttctat gacagaatta cttgatattt accgtactct taaatctaaa
     4681 acttgggtag acttgactca ccaaatcaat gaaaagagcc cgcatttccc tgcgctacca
     4741 gctctcgaaa agaaagccct atttacttac tcataaggat ggcttctttg tcaaacaatt
     4801 tacggttgtg gggcagtatg gaacccatat tgatcctcct attcattttg tagaaggagc
     4861 tcgttatctt gatgagattg atttgaaaga tttactcctg ccactctacg ttattgataa
     4921 atcagctgct attatagaaa ataatgacta tgagataact aagcaggata ttttagactt
     4981 tgaggctgaa tatggtccga tagcgccaga atcttttgtt gcctttcgtt cggattggtc
     5041 aaagcgctgg cccagtcaag atgccattcg taatctagac gaagatggag ttcaacgtac
     5101 accaggatgg agtcatgaag ctctagaata tttgattgaa gaacgtcagg tgaaagcagt
     5161 tggtcatgaa acattggata ccgattcagg agtatctgca gccaaacacg gaggaagttt
     5221 accagaagaa tactagctcc tatcaaaaga tatttatcag ttggaagtat tggcaaactt
     5281 ggatcaggta ccaccaacag gtgcattgat ttcaatcgct tttcctcatt gggagaaggc
     5341 ttcgggttca ccagtgcgag caattgctat attaccataa gaaaaagtcc agacagtgct
     5401 gaccttcatg tctggacttt ttcaattgag ttaacgcata gtaacaaatt cttcagaacc
     5461 tgtcggatgg atggcgacaa cggcatcaaa ttcttctttt gtagctccca ttttgatagc
     5521 aactgagaat ccttgaatca tttcatcgac accatagcca atgccgtgca gtccaacgac
     5581 tttttcattt tctcctacag ttactaattt taacttagcc atttggcgat tgttttctag
     5641 ggcagtgtac atagaagtaa aggctgaagt atagactttt acattgtcct gaccgtattg
     5701 ggcaatcgct tcgtcctcag ttagaccaac ggttccgata gcggggtgag aaaagacgac
     5761 agttgggatg gttgtgtaat ccatttttgc ggttggttta ttgttaaata aacgttctgc
     5821 taaaaggcga cctgccttga ttgcaacggg tgttaattct ttttcaccag ttacatcacc
     5881 gagggcatag atgcctggta cagctgtttc ttggtattca ttgacagaaa tatgtcctga
     5941 agaagtaagt tgaacactag ttgcttctag gttgagtttg tcaatatttg gtttacgtcc
     6001 aattgcccaa aggaccttct gagcaatatg gttactaccg tcagcaaagt taatctggat
     6061 ttgtccatca tcagtttttt caagactgac aggagttttc ccggtatgga gtggaaggtt
     6121 ggtacgttgc atttcttcca tcaaggcatc aacaatgtag gtatcaaagc tacgaagagg
     6181 gcgatttcca cgaacaaaga ggtcagtttg gacaccgagt ccatgtagga gaccggccat
     6241 ttccacagcg atataaccag caccaatcac agccacagaa gaaggtaatt cttcccatgc
     6301 aaagacatcg tcagatacaa tccctagctc actaccagga atagcaggga ctaccggttt
     6361 ggcgccagtt gcgatgacaa tgtgttttgc acgaatcagc tcaccattga cttcaaccgt
     6421 ttgattattc acaaaatgtg cgaaaccctg gaaaactttt actccgttat tgttaaaagt
     6481 attaccgtag gatgcgcgtg aacgctcaat ataggcttcg cgatttttac gcaaggtggc
     6541 aaaatcaaag gtcacttctt ggctggtaaa accatactct ggtccatagt ggtgaatggc
     6601 ttcagccact tgagaaccgt accacatgat ttttttggga acacagccaa ggttaacaca
     6661 ggtccctcct agatagcttc cctcaataac tgctgccttg gcaccgtata tagcagctcg
     6721 gttcatggta gcgatgcctc cgctcccacc tccgatagcg ataatatcaa acactttcat
     6781 aatttcctcc aataaaatca ttaactatat tcattgtaac gttttttggt gtgtcaatca
     6841 aaaatccgct acgttacctt acaaaaatgt aagtgggaaa attgccgaac tttttataaa
     6901 ataattagga aaaaacttgc atttgttttt gtatttgtgt attatataga caacacaaaa
     6961 taagtatgtt ttataaagaa aaggagtttg ttatgttaaa gacaaagaaa gcgaaaattg
     7021 ctttgtttgg cggccttgga gtagtcgcag ttgccctagt aggtggggcc ttgattttta
     7081 gcaatatgct aacgtcgtca ccatcttcag aagaagtagc catgagtatg acctatactg
     7141 ttgctaaaga gggctcgatt gcttcctcaa cgctacttac aggaacgatt accgcagcag
     7201 atgagcagta tgtttactat gatccttcta agggagattt gagtgaggtt ctggttgctc
     7261 caggtactca ggttgaagta ggtactcctt taattcgcta tgatgctaca gaattacagt
     7321 ctgctttgga tactgctgtt cgtggtcgtg ataagattgg tcgccaaatt gaagacttaa
     7381 gaaccaatgg tcaaactgtt caaacaactg gtgatgcaga aacagatgct gcagcaactg
     7441 ctactgcaca acgttcagta gatatgcagt tggcagactt gaatgattcg tacgcagatg
     7501 ctcaagctgc ggtcgataaa gcctatacag ccttaactga agcgacagta actagtaccg
     7561 ttgctggtac agttgttgaa gtgaataagg ctgtttctaa gaactctaca tctagccaga
     7621 cagttgtaca tattgtaaac caaggtagtt tgcaagtaac aggtagtttg acagaatacg
     7681 atttggctaa tattgcagtg gatcaagagg tcaaactgac tagcaaggtg tatcctgata
     7741 agacttggac agggaaaatt acctatattt caaactaccc atctagtgat caagctggtg
     7801 ccgcagctac aacaggaact ggtggttcag gcgctaaata tccatttaag gcagctctaa
     7861 ctagtgaatt aggtgagttg aagcaaggat tttctgtgaa tattgaggtt gtcaatacaa
     7921 gtaaaaacat tttaattccg gtgtccgcag ttgtgccaga agaagataaa aactatgtgt
     7981 ggaccttggt tgatgggaaa gctaagaaag tggaagtcac tctcggaaac gcagatgctt
     8041 tgaaccagga agtgactgct ggcgtagcag taggtgataa ggttattacc attccaactc
     8101 cggatctcga agatggcaaa gaggttgaag ccaatgaaga accagcttat tagattaacg
     8161 aatatcaata aatcctacaa aaatggtgat caagagctcc gagttctaaa ggatattgat
     8221 ttagaggtgg aagagggaga atttcttgcc attatgggac cttctggttc aggtaaatca
     8281 accttgatga acattattgg gcttttagat cgttccagtt caggaaacta ttggttagaa
     8341 ggtgaagaag tcagtcaact ttctgagaaa aaattagctc aggttcggaa tgaccaaatc
     8401 ggttttgtct tccaacaatt tttcctctta tcgaaactca acgccattca gaatgtagaa
     8461 ttacccttga tttattctgg agttccatca agcaaacgta aggaactagc gaaacaatat
     8521 cttgaaaaag tcgagttggc agagcgaatg acgcatttac catcggaatt gtctggtggt
     8581 caaaaacaac gggtggctat tgcgcgtgca ctagtcaata ctccggctat tattttagca
     8641 gatgagccta caggggcctt agatactaag acaggcgagc agattatgca acttctgacg
     8701 gagttgaata atgaaggaaa gaccattatc atggttacac atgagcctga gattgctgct
     8761 tacgcaaaac gaaaaatcgt cctacgtgat ggagtgatta ctgaagatag tcaaagggag
     8821 ggagtttaga tggaaaattg gaaattcgcc ctcaaatcta ttatggctca taaaatgagg
     8881 tcgctcctta cgatgttagg tatcatcatc ggtgtagctg cagttgtcat tattgtcgcc
     8941 atgggtacag ggatgtccaa gagcatagaa aagtctctag ctggtgatca aaataatgtt
     9001 caagtttact ttactgcctt ctccagatca agcacagcct tcggtccatt tggaacagta
     9061 tcgacggaag aaccgatctt agaggaagag ccagatttga cggattctac tttaaaagga
     9121 ttgctagaga ttgatggtgt atcgagctat tattccagca tcagtaacat ggctgaagtc
     9181 agctcaggca ataaaaaagc agagaatgtc accattactg gtgtcagtca gaattacttt
     9241 gctgtgaaag aatatgaaat tttagctggt cgtcaattta cagctaacga ttatagtcgt
     9301 ttttcacgta tgattatgct agatacggca ctagctgaga agttgtttgg ttctatagag
     9361 gactctctca atcagacgat tagtgtcaat agcaatgctt atctcgtaat aggagtttac
     9421 aaagatccaa aagcagggac agctatttat ggaatgaatt caggtggaaa tgccgtcatg
     9481 actaatacgc agctggctgc tgaaacaggc atgaaagaga attctcaaat ctatgtccat
     9541 gttgaagatg ttactcgggc aacagaagtt ggtcaggccg ctgcagatta cctaactaaa
     9601 gcgactggat tgaaagaagc gagatacgag atttatgata tgtcagctat gttggaagag
     9661 tttaaatctc aaatgtctgg tgttacctta tttatcggtg ctgttgcagg tatctccttg
     9721 ctggttggtg gtatcggtgt catgaatatc atgctggtgt cagtaactga gcggactcgt
     9781 gaaatcggtc ttcgaaaagc gcttggagca acccgaggca atatcttgat gcaattcttg
     9841 atagaagcca tggtcttgac cactcttggt ggagcgattg gtctcgcaat tgcccaaaca
     9901 atagtcttct tgctcaatgt gagcaaggcg atgggagaaa gaataactgc tgaagtttca
     9961 attccagttg ttctgggaag tctggctttc tcagcggtag ttggtattgt atttggcgta
    10021 ctacctgcca acaaagcatc taagcttgat ccaattgaag ctttgcgcta tgaataatct
    10081 attgaaacga ttaggttttc ctagtcgttt tattttgagc aaatcaattg attttatcac
    10141 taattagaga tgaaataaac gcatcaatta agaaatagtg atttcaaagg agaacacatc
    10201 atggtagaat taggcatttc aacatttggc gaaacaactc cactagaaaa aacaggggaa
    10261 acttacagcc acgacgagcg gattcgccag ctagtcaaag aaatcgaact agctgatgcg
    10321 gtgggacttg atgtttatgg gattggggag catcaccgag aagattttgc ggtttctgca
    10381 cctgagattg tgctggcagc tggtgccgtc aataccaagc atatcaaact aacatcagca
    10441 gtttcaatcc tttcctccat ggaccctgta aggctctatc agcagtacac caccattgat
    10501 gctttgtcaa atggcagagc ggaaatcatg gcgggtcgtg gctcctttac ggagtcattc
    10561 ccgttgtttg gctatgatct ccacgactat gaagaactct tcgatgaaaa gctagatatg
    10621 cttctggaaa tcgacaagga aaccaatctc aagtgggatg ggcattttac ccagtcagtc
    10681 gataataagc cagtctatcc acgtcctgtt caggaagatt ttccgatctg ggttgcaact
    10741 gggggcaatg tagagtccac catcaagatt gctaagaaag gcctgccaat cgtgtatgca
    10801 gtcattggtg caggtgccca tcgctttaaa cctttagttg atgcttatcg caaggttgct
    10861 cgcaactcag gtcatgatcc aaagaaaacg aaagttgcag cccattcttg gggttggatt
    10921 gcagacgacc atgacaaagc tgtagaagaa tattatcatc caaccaaagt aattacggac
    10981 aatattgcca aagaccgccc acattggagc gagatgacta aggcgcaata tctctactct
    11041 ctgacagacg aaggagcaac catcgtcgga gatccaaaac gagtcgctga aaaaattatc
    11101 aagactattg aaacacttga gttggaccgt ttcttcctcc atctcccaat cggctccatg
    11161 ccacatgaag atgtcctccg tgccatcgaa ctctacggaa aagaagtcgc gccaattgtc
    11221 cgagattatt ttgcaaaaaa agaagagaat cagtcccaag actgatgaga taaaaaggag
    11281 gggacgttat aatagcctca cttctctaaa gtggagtcaa catctcagcg cagtggttga
    11341 ttgggtttaa cagtccagtg gactgttaaa ggttggagat aagatttgcg aagcaaatct
    11401 cagcaattcg tgttttacac tccacatctg acctctacga ctgatgcgaa cagagttcgc
    11461 ttcatctcca acctcaaact gtctcccaga cagtttgagc tgtgcggggg tgggagtgaa
    11521 acagtctggg gatagactgt ttcagctcaa caactggaaa tatgaacttg ttgacgaact
    11581 ctctatgatt atttgagttc tgtccactcc caatcctaaa tattttttat aatctcctga
    11641 aaaacctgac catgtggttg ggattttttg catgcaaaaa cactcttgga atttccaaga
    11701 gtgttttaag taatggtgtt aaacagtttg taatttttat ttcatcgtcg ggagtgaaaa
    11761 ggtctgggag accttttcag ccaatcgcca agaaacatga aagcgattgg aacatcctag
    11821 tgaaaacaaa tctagcaaac tactcttgct tatttcatag tcggaaatag gagtacatcc
    11881 cgaatagtag tagtatctgt cagaagcatg cagaggcggt cgataccgat tcccaggccg
    11941 cctgttggtg gcataccgta ttcgagggct tcgatgtagt cgtagtcaac gcctgtcgct
    12001 tcgtcgtcac cgagttcttt agtcttggct tgggcttcaa agcgttccaa ttggtcaatt
    12061 gggtcgttca attctgtaaa ggcgtttccg tattccttgg tcatgatgaa gagctcgaag
    12121 cggtctgtga agcgtgggtc ttcatcgttt ttcttagcca gtggagatac ggctactggg
    12181 tgaccataga caaaggttgg ttggataaga gttgcttcaa cgtattcttc aaagaagctg
    12241 ttgatgattt gaccaacttc tgtatggtgt ttttccacag ggaccttgtg ctcagctgca
    12301 agggcttttg cttcctcgaa gctcatctct tgccagaagt ccacgccagt ttgttcctta
    12361 atagcgtcaa ccatgtggat acgcttgaat ggctcgtgga tagcaatttc agtgccctga
    12421 taagtcacag gaccatcacc aacaacagcc ttggcagtgt gctgtataat accttcggtc
    12481 aagtccatga tgtcttggta gtctgcataa gcttggtaaa cctcaatgga agtgaactca
    12541 gggttgtggg tcgcatccat accttcgtta cggaagatac ggccgatttc ataaacgcgt
    12601 tccataccgc cgacaatcag gcgtttgagg tggagttctg tcgcgatacg aaggaccatg
    12661 tcaatgtttt gggcattgtg gtgggtgatg aatggacgag ctgcagcacc accagcttcg
    12721 ttgtgaagaa ctggtgtttc cacttccaag aaaccaagtc cgtcaaggta acggcggatt
    12781 tctgagataa tttttgagcg ggtcacaaag cgatcaaagc tctcgcggtt agtaatcaag
    12841 tccaaataac gtttgcggta gcgggtctcg atgtctgtca aaccgtggaa tttttctggc
    12901 aatggacgga gcgctttaga taagtgagtc aacttgcgag catggataga caactcacca
    12961 acgtttgtct tgaagacatc tccctcgaca ccgataaagt caccaaggtc tgcttttttg
    13021 aagatttcat aattttcttc gccaacatca tccttacgaa cgtagatttg aatctggcct
    13081 tcgcggtctt gaatgtgagc aaaacctgcc ttacccttac cacgcttggt cataatacga
    13141 cctgcaatga ttgctgtttg ccctaattct tccaagtctt cttttgattt gtcttcgtac
    13201 tgggccttca attcagctga gttggcagaa cgctcaaaac gttttccaaa tgggtcaatg
    13261 ccctgttcgg caagagccgt cattttctca cgacggacaa tctgttggtc atttaattct
    13321 tcaaaatgtt cagttgacat aaaaatttta ttcctccaaa gtctgttatt tctattttat
    13381 catagaagag agaaaaaatc agtataaatt gctaatagga taggaaaaaa cagcagaaaa
    13441 ttctactgtt ttgctttata gctggtatca ttccaagcta attctgtaaa gttttcgaaa
    13501 tcgcttgttt ctaaaatagt aaccgatgca ttatccaaac cgcctcggtg acgcagttgg
    13561 gctggtggaa aaccaagcag gcgatggata gaagcggtta gaaaggcacc atgactgacg
    13621 atgaggactg tttccatgtc ttgccctttc agcgattgga caaagtccac cattcgttga
    13681 gttacctgcc gaacgctctc agcttcaaat aagtcattgt taaaaagtgc tagattatgt
    13741 ttgagtgccc aagcttgttt ggggtagatg gcacgaaaaa atgccatctt gcttccttct
    13801 aacgttccaa aattccattc acgtaattat tgcgtggtta gaacggtttg gcaatggtga
    13861 ttggctttag caatttctac ggcagtgcta cgtgcgcgct gcaaatcact agaataaata
    13921 gcatcaaagg ggatttcagc aaggtatttg cctagttttt ctaaatcccg ataagattca
    13981 ggcaaaagag ctgaatcacc agaatagcct tgaaagcgcc cctccaaatt ccgttcggtc
    14041 ttaccgtgac ggacaaagta gattttcaag tctagtccct cccctcaatc aaatcgacaa
    14101 agtcagcttt tacaaaatca gctaatacct gactgtcaat tcgaatcgac cgaccaattt
    14161 ctccagcaga gacaatcaag tgaccgagtt caagggcaga ttgatcaatg tagattggga
    14221 aattatgctt ctgacgaatg cccacgggat tgttggcacc gtggacgtag ccagttgtcg
    14281 tttccaagtc cttttgcgga atcatactca cttttttatt gccagagatc ttggctaatt
    14341 ttttctcaga caggtgttct gtgattggga caatgccgat aattggtcca gttttatctc
    14401 cagttaatgc cagtgttttg taaatttcgt gttcttcaat tcctggtgga agttgacctt
    14461 cgaaggcatt taaaacaagg ctatcatgat tgatcttagc cttgtccaat atttggtcca
    14521 ccaaggtttt ctttatttta accttttttg ccattattta tatttttcct caagtttact
    14581 catccaaagc cctaaccatg ctcctagtat aaataggaca atagcagcaa tccatgtaac
    14641 aaagttcgct ccagcatagg aaatagactc tttattactt gcttgtggaa tcaaaattag
    14701 gagagtagaa gatgagacga taccaataat aaagtgataa acacgtgaat ggtatacttt
    14761 cagagcgtgg tccattgctt ttgaaaatgc aaccattgca agaacgccac cgattgcgat
    14821 tggtaggaag gtaccgaata ggtcaagtga tttgaaacca tttagcattg gcgtgtagat
    14881 acccaaaatc aatagtaagt ttgaagggct gagtccagga acaagtaccc ctaaagcaat
    14941 caaggtacct gccaatacaa aactagcaaa agtagctgga agtgtcccga caagatcgct
    15001 caaattgtaa agtaggaggc cagaaataat gaatgtccca atcaaccaag ccaaatcaat
    15061 gctatctcgt ttgctctttt gagttgattc tttaagtagg ctaggaatag ttcccacgat
    15121 tgctcccgca aatgcccaca aaactgggac ttggaaatgt tgcaataaaa attctacagg
    15181 gaaagagaaa agtgcgattc ccaaaattcc accaattcct acaggaacaa aataaagaaa
    15241 gttttccttg aaatcttttc gaatatttgc taaaaatcca attagtcgct catagattcc
    15301 gaggatagca gcgagtaccc ccccagagac accaggtaag ataaagccga gtgcgataat
    15361 cataccttta ataattcttg aaatccaaga agccataatt tcctccaaaa tgtattataa
    15421 cgttcctatt atagcataaa gcaagtaaaa ataattaaaa agactgcctg ttagataaaa
    15481 tccagcaggc agttttggtt aaagattgta aaaacctggt cttgctttat agagtaaaat
    15541 gagcagaata gaggcaattg cagtggaagg aagaatatag ctgatattgt aggtaaagga
    15601 atagaaccag acaggcatac catctggagc gtagtctgcg tagaaaataa ttcctgataa
    15661 gaaggtggct aacaatcgga tgcttcctcc tagaaaacta gccagagtaa tgatgagagt
    15721 agcattttta agatcttttt gtttggatag ggtgggtgcc actaaacctg ctaaaccaat
    15781 tccggcgtaa gaaagtgcgt agtcaagaaa aacttgaaaa acgtttagga agtagccacc
    15841 gtagaaaagt tgaatggttc cgacaatacc accagtaaga acccccaccc ctagtccgtg
    15901 gcgcagagca agcagtaaga ttggaagcat gactaaagat acagatccac cttggggcat
    15961 tgtaaagagc gggattttgt caaagacgag ggcaatggca gaaaagatag taacttcagc
    16021 taggatgggt aattttgatg tgggcataaa aagactcctt tgtagatggt caacaaagga
    16081 gtgtttatag cagagtatag tatcattcta taaagcacac aattcctacg ctagtgttaa
    16141 ctagatcagg ttcgaggatt tcgcagttgc gatctcagcc gaagcactcc tttgtgtata
    16201 tgtaattagt gaaattgtat cattttattc gggtgttgta aatagtttct gataaacttc
    16261 attaggatcc ttcagggtag taataatgtt gaggtctagg tggtgctcaa agccgtttac
    16321 ataaccacgt gaggtatatt ggtgaagatc gtagttcaag tcagtatttg gagcggcatt
    16381 gtagtagcca gaatcatcac cgtatgtagg aatccaaatg gcgtcaaatc tatccacatt
    16441 gatgctgtgg tcctccatga aataggttcc gacgtaaatt ccaatgtttt tagcgccgag
    16501 agtttctaac tcttttcgaa aagcctccac tccagcattc atatcatcca tggtatattc
    16561 ttcaacgtct aaccaataga aggttggctt gtatttattt gcggccttat aaaagcttcg
    16621 agcttcctcc tgcatgttct caatgctatt tcctgtgaca taggcataga cagcaacagg
    16681 tatatttctt gcttggaatt ctttaatatg ggtatcaaag gatttatcta acccattttt
    16741 atcagtcgca gtattttcgt ttttagtgtg tgatccactt tgaatacgaa cgattgcgcc
    16801 agaaatgttt ttactaaggg tatcgtagtc aatttcagac ggtctctgcc aagcagaaat
    16861 gtcgacgata ggtttcattt cgagcgcatt tgcaatgtaa taacttgaac tagaatttgt
    16921 agttttagaa cttgtactgg tgtttgattg tgtgactgcg ggttcgttag ctacctccgt
    16981 cttatttacg gtatttccag tcaccccagt gatgaaaaca agagcaaaga aggataggaa
    17041 aaaaccaaca acaatcatcg gatttagttt ctttctcatc tcctcaattc taccgtaaaa
    17101 tcatgaaaaa atcaaaaaaa tacgattcaa gaaatagtaa agattatctg aacttgacat
    17161 aataaaggga atagggtagg ctaaattggt atgcatgaca aaagtgaaaa agtaagacgt
    17221 gttagtcagc tagttgttct gtcctctttg acgattgttt taagggtggt ttttggggca
    17281 tttcccaata taaagcctct cacggctatt ttcctcattg ttctgtccta tttcagtttg
    17341 atggagagct ggatattgat gatactagta atggtaggga gtggcttgct ttttggattg
    17401 ggcacagttg ttttgtggca agttcttagt tttgctttta tccaatttat atggtggttg
    17461 gcaatcggtc cttttatcaa aaaagagaac attcccttgt ggctacagag tcttctggcg
    17521 gggattcttg tctttttgta tgggctaacg ataagttttt tatctgctac acagtttggg
    17581 acttccccta tcgtcttctg gcttaatggt ctagtctttg atagtttaca cgctgcttca
    17641 acagttttgt tttatccaat tattgattat atttttagga gattttataa atgaaaaaaa
    17701 ttagtattat gttgacggtt gtcttctcgc tcttcttggt tgcttgtaca aacactactg
    17761 agaaacagag tgactcggct atcgaagtca ctttgcagat tacaaccaag gacaaagaga
    17821 atcgtcagga agtcactgcg gaagaaggtg atagtgtcat ggatgtgctt aaagaacatc
    17881 atgacattgt agaagacaat ggtttgataa cagaaattga tggtgttagc caagatgcga
    17941 gtaccaatac atactgg
//
LOCUS       DATUCG010000039        17724 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.040, whole genome shotgun sequence.
ACCESSION   DATUCG010000039 DATUCG010000000
VERSION     DATUCG010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17724
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.040"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(1..3112)
                     /locus_tag="VCA20_001039"
                     /pseudo
     CDS             complement(1..3112)
                     /locus_tag="VCA20_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937143.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc carboxypeptidase"
     gene            3328..4875
                     /gene="cls"
                     /locus_tag="VCA20_001040"
     CDS             3328..4875
                     /gene="cls"
                     /locus_tag="VCA20_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEP1834880.1"
                     /translation="MEKLWKIIYSRTFIVISLIILTIFLILWLVGSAATYMPALLIVL
                     QIFSIFVAISIINRPMNTSFKLTWIIFVIGIPIFGALFYFILQSNIETKRYRKNFHKQ
                     AQRLREYSKTSEKVMKALEQEDKEQLKLAHFMSEYVGYPIHTNTDAVYFSTGQEKFEA
                     LLEELKKAEQYIFMEYFIVDMGYMWDSILDILKEKAAQGVEVRFMYDGMNSLTNLPYN
                     YYKTLRKYGIKAKVFSQIIPALSTVQNNRDHRKIAIIDGKVAFTGGINIADEYINKKV
                     RFGYWKDAAIMIRGEAVANFTLMFLQMWNYNEKTETDDLKYLKTHKDLETETVDAEGF
                     FLPYGENPFDGDEVAKRVCLDMIQSATDYIYIMTPYLVLDDEMIDNLTYTAKRGVEVR
                     ILLPHIYDKKSAYLAARTDFPTYLEAGIEIFEFQPGFVHSKVVLVDDKKATVGTVNMD
                     FRSFYLNFECGLYIYNHSAVLAAIKDDFTQSFKKSQQINLLTFHKTYSWYKRLGGALL
                     RIISPLL"
     gene            5033..7246
                     /gene="recJ"
                     /locus_tag="VCA20_001041"
     CDS             5033..7246
                     /gene="recJ"
                     /locus_tag="VCA20_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEP1834881.1"
                     /translation="MIKPNYDWQLLTGFSDEQFIKIAKKEGVDPVAAKLLYERGIHSA
                     EELHTFLQPSLEDLHDPYLLHDMDKAVERIRRAIEDYEQILIYGDYDADGMTSASILK
                     ETLEEMGAEVQVYLPNRFTDGYGPNQSVYKYFIKQQGISLIVTVDNGVAGHEAIAYAQ
                     EMGVDVVVTDHHSMQETLPNAYAIVHPEHPEGNYPFKHLAGCGVAFKLACALLETVHA
                     DLLDLVAIGTIADMVSLTDENRVMVKYGLSLLKQTERAGLQELIKIAGIDIDSIDEET
                     VGFQLSPRLNALGRLDDPNPAIELLTGFDDEEAHQIALMIDSKNVERKDVVQAIYDEA
                     KTMLRHDRPVQVLAKEGWNPGVLGIVAGRLLEELQQPVIVLSIEDGKAKGSARSVEAV
                     DIFKALKDHQDLFIAFGGHAGAAGMTLEVDKLEELALTLTDYIIENKLDLSSKSSLVL
                     DEELDLEELTLDTLKSFEKLAPYGMDNKKPVFYIRDFQVESARTMGQNNAHLKLRITK
                     GAAGFDVVAFGKGNLALEFSQAKGLELAVTLSVNQWNGNTSLQLMLVDARVNGVQLYN
                     IRGKQHPIPAGVPILDIENPVADNKAIVLANLPEDLSSLRSYFQEREFEAIYFKNEIA
                     KPYYLDGYGSREQFAKLYKTIYQFDEFDVRYKLKYLAGYLKIKDSLLVKMIQIFQELE
                     FVTITDGVMRVNKEAQKREISESQIYQELKETVIQQELMALGTVQEIYEWLCGRV"
     gene            7225..8114
                     /locus_tag="VCA20_001042"
                     /pseudo
     CDS             7225..8114
                     /locus_tag="VCA20_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917262.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3114 domain-containing protein"
     gene            8662..9174
                     /locus_tag="VCA20_001043"
     CDS             8662..9174
                     /locus_tag="VCA20_001043"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEP1834882.1"
                     /translation="MNLKDYIATIPNYPKEGIEFRDISPLMADGNAYSYAIREIVQYA
                     TDKQIDMIVGPEARGFIVGCPVAFELGIGFAPVRKPGKLPREVISADYEKEYGVDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGIVAGCAFLIELDDLKGREAIG
                     DYDYKVLMHY"
     gene            9278..10219
                     /locus_tag="VCA20_001044"
     CDS             9278..10219
                     /locus_tag="VCA20_001044"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HEP1834883.1"
                     /translation="MPIKIEKSLPAVEILRKENIFVMDSDRASHQDIRPIKILILNLM
                     PQKIATETQLLRLLANTPLQLEVEFLYMASHESKNTHSDHLKQFYKTFEQVRDSYFDG
                     LIVTGAPVENLPFEEVDYWQELTQVFDWSKTHVYSTLHICWGAQAGLYARYGVPKHSM
                     SRKLSGVYCQEVTNPTSPLMRGFDDEFRSPHSRYTEVREADIAHLSGLTIFSQGPEVG
                     LSILASKDLREIYSFGHLEYDRDTLAKEYQRDCIAGKNPHLPENYFKEDDPTSKPSLS
                     WNLPAAQFFTNWVNYAVYQETPYDWHIFEQSAQSASL"
     gene            10229..10903
                     /locus_tag="VCA20_001045"
     CDS             10229..10903
                     /locus_tag="VCA20_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HEP1834884.1"
                     /translation="MNYSQQFRQGHLVLPAAILFHYQELFPSADDFLIWQFFLYQNSS
                     AIESLAPSEIAQATGKTVAQVNQAIENLQDAGLLEFKTISIAGEIEMIFDALPAFEKL
                     DVLLTPKQAVEIVQPENDLKTLVGDFERELGRFLSPFEIEDLQKTIEDDKTSIDLVRA
                     ALKEAVFNNKTNWKYIQAILRNWRREGITTVAQVEAKNAEREIQTPKNVTVSSDFLDA
                     MDLWKD"
     gene            10905..11588
                     /locus_tag="VCA20_001046"
     CDS             10905..11588
                     /locus_tag="VCA20_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEP1834885.1"
                     /translation="METKLSKRLETVASFVPQGARLVDVGSDHAYLPLFLVEQGRIDF
                     AIAGEVVQGPYQSALQNVEQAGQTEKILVRLANGLAAFEKSDQMTAVTIAGMGGRLIA
                     EILEAGKEKLATIERLILQPNNREDDVRRWLEKNGFRLVAEKILEENGKLYEVLVAES
                     GQMRLSEVEHRFGPYLLEEASPVFVKKWRRELEKLSFALEQVPSERQGDRFAISQKIQ
                     QIQEVVHVS"
     gene            11578..12372
                     /locus_tag="VCA20_001047"
     CDS             11578..12372
                     /locus_tag="VCA20_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEP1834886.1"
                     /translation="MLASQVIERYQAFCPPSLAMEGDIKGLQIGTLNKEIQRVMVALD
                     IRETTVAEAIDKKVDLIIVKHAPIFRPIKDLVADNPQTKMYLDLIKHDIAVYVSHTDI
                     DVVEGGLNDWFCELLEIQDTTYLIETAEGQGLGRVGTIAEQTLEELALKVKSVFDLDA
                     VRLVIYGHQAKQYVSRVAICGGSGGSYYHEALAKGADVYITGDIYYHTGQDMLTQGLL
                     AIDPGHHIEALFIKKITELLETWKAKEGWNVEILPSTVSTNPFRHL"
     gene            12548..13372
                     /locus_tag="VCA20_001048"
     CDS             12548..13372
                     /locus_tag="VCA20_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009659387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEP1834887.1"
                     /translation="MTWVTNQSVVLQALLGGLFTWFCTIVGSAVVFFFKTVSRRLLDT
                     MLGFAAGVMIAASFWSLLAPSIEYAESSYGNLAWIPVAVGFAAGGIFLRLVDAWVPHL
                     HLGNDKDKAEGGGEKERKNLSKTALLFLAITIHNIPEGLAVGVTFGALASNYSPAAFI
                     GAIGLAIGIGIQNIPEGAALAIPIRTDGASRWKAFYWGSMSAIVEPIAAVIGAFAVTF
                     MTPILPYALSFAAGAMIFVVVEELIPESQTNGNTDIATLGLMAGFIIMMILDVALG"
     gene            13470..14339
                     /gene="rfbA"
                     /locus_tag="VCA20_001049"
     CDS             13470..14339
                     /gene="rfbA"
                     /locus_tag="VCA20_001049"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002276675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEP1834888.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPIYDKPMIYYPLSTLMLA
                     GIKDILIISTPQDLPRFKDMLGDGSELGISLSYAEQPSPDGLAQAFIIGEEFIGDDNV
                     ALILGDNIYHGNGLTKMLQRAASKEKGATVFGYQVKDPERFGVVEFDADMNAISIEEK
                     PEEPKSNFAVTGLYFYDNDVVEIAKNIKPSPRGELEITDVNKAYLERGDLSVELMGRG
                     FAWLDTGTHESLLEAAQYIETVQRLQNVQVANLEEIAYRMGYITKEQVHELAQPLKKN
                     EYGQYLLRLIGEA"
     gene            14339..14932
                     /locus_tag="VCA20_001050"
     CDS             14339..14932
                     /locus_tag="VCA20_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018029578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEP1834889.1"
                     /translation="MTENFFGKTLAARPVEAIPGMLEFDIPVHGDNRGWFKENFQKEK
                     MLPLGFPESFFAEGKLQNNVSFSRKNVLRGLHAEPWDKYISVADGGKVLGTWVDLREG
                     ETFGNTYQTVIDASKGIFVPRGVANGFQVLSDFVAYSYLVNDYWALELKPKYAFVNYA
                     DPSLDIQWENLEEAEVSEADKNHPLLKDVKPLRKEDL"
     gene            14932..15417
                     /locus_tag="VCA20_001051"
     CDS             14932..15417
                     /locus_tag="VCA20_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEP1834890.1"
                     /translation="MFQAFLEIAEQLNKLGITPLLMGSVGLERQTGRDWQARDLDIHV
                     PSDPRGWEASDEERIYRADELIVLMNQLGYVLVDRHEHEFHKDGLSVEFGGLHSLPEF
                     ADVELEDLEELETAGVRYYLPTLEQYLKIYQASSKDSYRAENNNQKDFAKIAYLKEIL
                     K"
     gene            15607..16653
                     /gene="rfbB"
                     /locus_tag="VCA20_001052"
     CDS             15607..16653
                     /gene="rfbB"
                     /locus_tag="VCA20_001052"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEP1834891.1"
                     /translation="MSQFKNIIVTGGAGFIGSNFVHYVYNNHPDVHVTVLDKLTYAGN
                     KANLEAILGDRVELVVGDIADVELVDKLAAKADAIVHYAAESHNDNSLNDPSPFIHTN
                     FIGTYTLLEAARKYDIRFHHVSTDEVYGDLPLREDLPGHGEGPGEKFTAETNYNPSSP
                     YSSTKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILAGIKPKLY
                     GEGKNVRDWIHTNDHSTGVWAILTKGRMGETYLIGADGEKNNKEVLELILEKMGQPKD
                     AYDHVTDRAGHDLRYAIDASKLRDELGWTPQFTDFSQGLEETIQWYTDNQDWWKAEKE
                     AVEANYAMTQEVIK"
     gene            16708..17559
                     /gene="rfbD"
                     /locus_tag="VCA20_001053"
     CDS             16708..17559
                     /gene="rfbD"
                     /locus_tag="VCA20_001053"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019780653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEP1834892.1"
                     /translation="MILITGANGQLGTELRYLLDERGVEYVAADVAEMDITDADKVDA
                     FFAEVKPSVVYHCAAYTAVDMAEDEGKELNYKINVTGSENVAKAAAKYGATLVYISTD
                     YVFNGDLPVGQEWQVYDQPDPQSEYGRTKRLGEEAVEKFADKFYTVRTAWVFGNYGKN
                     FVFTMQNLAETRDTLTVVNDQHGRPTWTRTLAEFMVHLVNTKQAFGYYHLSNDAAEDT
                     TWFDFATEILKDTNTKVLPVDSSQFPAKAKRPFNSTMSLDKAKATGFVIPTWQEALEA
                     FYKQEKR"
BASE COUNT         5159 a         3352 c         3995 g         5218 t
ORIGIN      
        1 tgttttttca cctgtttgag gtagttgttt tgcctcggta gacttggtgg tatgtacaac
       61 tgtagtagtt tttggcgact catccagaat attttttggc atttcaggtt gtggtacaag
      121 aatttctaca gctggtaagt cttctaaggt tgctaattga ctaccaaata gggcgttgga
      181 cacccaacgg aagaagtgaa cagggtggac cttgtttgtt ggattacctg catagataaa
      241 gacaggttgg tcttgataat ttccagcgat ggcgacaatt ttattggcta atttgtcatg
      301 accaggccac caacctgcga tataataatc tttgtcagca attttaacaa gtgttttaaa
      361 tccttcagga atgccttcaa tccaattacc ggagttggaa tagaaaagtc cattcatggc
      421 gtagccactt gttaatggat ctttatcatc aataatagcc cgcatgagac cttcatagtc
      481 accaccatct gtaaattgtt ccgcattgaa acctgctaag attcctaatt cctccagttt
      541 ttgcatggcg acaccaccta cgatgataat tggtttctta ccaaatacac tggcatcgaa
      601 ttggtctgat tcgagaatga tagcatcagc ctcatctgcg ctattgacaa tttcaaagcc
      661 cattcgttct accgcaagtg cagcatttgc aagaatggca aagtcgcctg cccaactata
      721 agagtgagaa ggagcgtaca cctttattgc ttttaattct tgtccaagag gcttcttata
      781 gagaggctcg ccgtatagag cataggtgtc cagtaagtag ctaaattggt tagtttccat
      841 gatgtagcca tcgtctgtaa ggtagacctt agcgcctgat ttgatggctt gattaatggc
      901 ctgaacagcg ctttcagatg tgtttgcgac gacatagtaa ggcgctttga aatcaatttc
      961 cgtagtacgt ggagcttttg tccatgtgat gctacctaat ttgccgctaa aggcgttttc
     1021 ttcaaataca gccttggcgc taaagccttt catatctggg aagttgacca ccaattcagc
     1081 atacatggct cccaggcaga ttcatcagaa ccagcataga ggacatggtt agcgaatcct
     1141 cgtttggctt gagccatatc gacaactagg tcgcccttgc ggaagtttcc aacatcttca
     1201 gttaattcct tgacaacaac cccattacga ttgaaatatt caatcatctt aaatgcttct
     1261 tgcatatcgt tgtgtttatc gagcgtcata ggaatcacat agtagtctgg gaagaatttt
     1321 ggctggtctt ttttgacacg tccgacaaca gccccgtctg gtccgactag ttcagactct
     1381 gcttttggat cttctacctt attgacaccg cgtgaatagt atttgagacg catttccata
     1441 agactgtctg gcttagttgc catattgtga acaccgccca aaacagcaaa gaatcccgcc
     1501 ttgaatgatt cttgatttcc ttctggaatt tcaatcgtgt gtccaagaat accgtggtac
     1561 attgcataca ccgctgtata gcctgagaag gaatcgtccc aaccatctcc ccagtggact
     1621 ttagggatgg tgtagcgttc atatttagaa tttgcaatac ctgctcggcc catttcatga
     1681 gctttttcca gcataatatc ggctagtaaa tcatattcaa agttcgggtc atgtggcgga
     1741 gtggcaggtt cgatgagaaa tccagataca aaaccatgaa tatctaaaac agatattgga
     1801 ttccatttgt ggatttgacg gacgatggct tgtgtttctg gattcacttg gaaaccagta
     1861 tcacggttag ggtccaaacc atttactagt gaacgcaagt ttttaacatc gccatcagga
     1921 ttttctgtga aattgaacaa gaagataaat ttatctaaca aatctgtcac ttttaaatga
     1981 agagtaacag gatttccatc ggcatctgta gatgggaagg tgatggtatc tttttgtgca
     2041 aattctttga ataggctagt taccacatct atgccgggtt gttcgtcagc atgagtgttg
     2101 ttgatgagaa taggtaattt atagtcgaag gttccagctt gcaactgttt cagcatctgg
     2161 tcggggtggg tcagcatgag tggcgttgtt tcagtaagat atttatcaat acttgcttga
     2221 tctttagcca caacagccat tttaatatct ctaccttcag ctgattgacc aattgactca
     2281 atccttacta ggcggtcggt cgcagcatta tttttcgttt cctcaatctc tgtcaacatt
     2341 tcctcatgtg tacggtaggc ttcataagga cggaaatgaa gagtttttga aattatcagg
     2401 tttccatcgg cactagtacc ttccaacagc atatctttta taaaatgtcg ataggtacgg
     2461 cgaatattac tcggtgtccg tttactcaaa tcttctccaa agagagctgc gagctggata
     2521 ttcaaatgaa gttggttatc ctgtctgctt tcttctactg tgataaatgt atcgcctgtg
     2581 aaatctccct ctttcaaatt ccaggttttc cattcggaaa taggctttcc atccaatgtc
     2641 cattctattt tttcagtcgt atctggaaga gtgacggatt ctgtgatagt gactgcttcc
     2701 gatagataga cagtttttgt ttcgagtgta actgtatcaa tagtcggtac ggcagatttt
     2761 tttctgtagt attctgatta tcattattag ctgctctgag cgtagtttcc tcggtattgg
     2821 gctcggaaga tgttgtatct tcttctgtgg aaacaggtgc aggagaagta tctgctattt
     2881 cttcagtttg tttttctgtc tctttccctt ctacaagatt atctgtagct tcgcttgtgc
     2941 tagttttcgt aataggagtc tcttcaggca gtggagtcac tgtttcacta gtagtaggtg
     3001 gtatctcctc tgtatttgcg aagaccgttc cctgagcgag agatgcagct aaaagaactg
     3061 ttgaggcaag taggacttga ttcaacagcc ttttcttata tgaaagtttc atgcgaactt
     3121 cctccttttg ataatgtgta cggggtaata aattaaaacg ggttatttta tgcctatctt
     3181 atcaaaaaat atatgtcttt acaatataaa ttgaatatgt ttatatattt aaaatattca
     3241 gaaatatcga atcatagaga ctatttattt tgagtaggat aatgaactaa aatctgttat
     3301 aatggtaaaa aaataaggga gagatgtatg gaaaaacttt ggaaaattat atatagtcgg
     3361 acatttattg tcattagttt gattattcta accattttcc ttattctttg gttggtaggt
     3421 tcggcagcaa cctatatgcc tgctttattg attgttttgc aaattttttc gatttttgta
     3481 gctatttcaa tcatcaatag accgatgaat accagtttta agttaacctg gattattttt
     3541 gtcattggta ttccgatttt tggtgctcta ttctatttta ttttgcagtc caacattgaa
     3601 accaaacgtt atcgtaaaaa ttttcataag caagcacaaa gacttcggga gtatagtaag
     3661 acgtctgaaa aggtcatgaa ggcattggag caggaagata aggagcaact gaaattagct
     3721 cacttcatga gtgagtacgt tggctaccca attcatacca atacggatgc cgtctatttt
     3781 tcaacaggtc aggaaaaatt tgaagctcta ctagaagaat tgaaaaaggc agagcagtat
     3841 attttcatgg agtactttat tgtcgatatg ggatatatgt gggattctat ccttgacatt
     3901 ttgaaggaaa aggcagcgca aggggtggaa gttcgtttta tgtatgatgg gatgaattca
     3961 ctgaccaatc ttccctataa ttactataaa accttgcgaa aatatgggat aaaagccaaa
     4021 gtattttctc aaattatccc agccttatct acggtgcaaa ataaccgtga ccatagaaaa
     4081 attgcgatta ttgatggaaa ggttgccttt accggtggca ttaatatcgc agatgaatac
     4141 atcaacaaga aagtgcgttt tggttactgg aaagacgcag cgattatgat taggggagaa
     4201 gctgttgcaa actttacgtt gatgttcctt caaatgtgga attacaatga aaagacagag
     4261 acagatgacc taaaatacct caaaacgcat aaagatttag agacggaaac cgttgatgcc
     4321 gaaggtttct tccttccgta tggcgagaat ccatttgatg gagatgaggt agccaaacga
     4381 gtctgcctag acatgattca gtcggcaacg gattatattt atatcatgac accctaccta
     4441 gtgttggatg atgaaatgat tgataatttg acttatactg cgaagcgtgg agtcgaggtt
     4501 cgcattctgc taccgcatat ctatgataaa aaatcagcct atttggctgc tcgtactgat
     4561 tttccgacat acttggaagc agggattgaa atctttgaat ttcagcctgg atttgtccat
     4621 tccaaagttg ttttggtcga tgataagaaa gctactgttg gtacggtgaa tatggatttt
     4681 cgctcttttt atcttaattt tgaatgcggt ctctatattt acaatcactc ggcagttttg
     4741 gcagctatta aggatgactt tactcagtcc ttcaaaaaat cacagcaaat taaccttcta
     4801 accttccata aaacgtactc ttggtataag cgtttgggag gagccctact gcgaattatt
     4861 tcgccgttat tataaatcgc aatattcgtt cttcatggag caagggatcg acattctgtt
     4921 ttggtcttgc cttgctccat ttttcttgga aaatgatata atagaaagat agtttatact
     4981 cggaaaagtt acaggctact tgtgattttg agtattaagg agtaaaaaaa acgtgataaa
     5041 acccaattat gactggcaat tattaaccgg tttttctgat gaacaattta tcaaaattgc
     5101 taaaaaagaa ggagtggacc cggtagcggc taagttgctg tatgaacgtg gcatccattc
     5161 ggcggaagaa ttgcatactt tcttacaacc aagcttagaa gaccttcacg atccctattt
     5221 actacatgat atggacaagg cggtagagcg gattcgccga gcaatcgaag attacgaaca
     5281 aattttgatt tatggagatt atgacgcaga cggcatgacc tcggcatcta ttctgaaaga
     5341 aactctggag gaaatggggg cagaagtcca agtttatctt cccaaccgtt tcacagatgg
     5401 ctatgggccg aatcagtcag tttacaagta ttttatcaag caacagggga tttctcttat
     5461 tgtgacggtg gacaatggcg tagcgggtca tgaagcgatt gcctatgccc aggaaatggg
     5521 tgtcgatgtt gtcgtgacag accaccattc catgcaggaa acccttccca atgcctatgc
     5581 gattgtacat ccagagcatc cagaaggaaa ttatcccttc aaacatttgg cgggctgcgg
     5641 cgtggctttt aaattggcct gtgctcttct tgaaactgtt catgcagact tgctggattt
     5701 ggtcgcaatt ggcaccatcg ctgatatggt tagcttgacc gatgagaatc gtgtcatggt
     5761 caaatatggt ctctcgcttc tcaaacagac tgagcgagca ggtttgcaag agttgataaa
     5821 gatagctggt attgacatag acagtattga tgaagaaaca gttggtttcc aactttctcc
     5881 gaggttgaat gcccttggtc gactcgacga tcctaatcca gccatcgaat tactgaccgg
     5941 ttttgatgac gaagaggccc atcaaattgc cctcatgatt gacagtaaaa atgtagaacg
     6001 caaagacgtc gtccaagcaa tttacgatga agcaaaaacc atgctccgtc atgatagacc
     6061 tgttcaagtg ctggctaaag aaggttggaa cccaggtgtt ttagggattg tagcaggacg
     6121 cctactagaa gaattgcagc aacctgttat cgttttgtcg attgaagatg gtaaggcaaa
     6181 gggttcggct cgttctgttg aagcggttga cattttcaag gcccttaaag atcatcagga
     6241 cctatttatc gcctttggtg gtcatgctgg tgcagcaggt atgaccttgg aagttgacaa
     6301 acttgaagag cttgctctga ccttgacaga ctacattatc gaaaataaat tggatttatc
     6361 cagcaagtct tcgctggtct tagatgaaga attggattta gaggaattaa ccctagatac
     6421 tctaaaatct ttcgagaagc ttgcgcctta tggcatggat aataaaaagc ctgtattcta
     6481 catcagagac tttcaggtgg aatcagcaag aaccatgggg cagaataatg cccatttgaa
     6541 actacgcatt actaaaggtg ctgcaggatt tgatgtcgtg gcttttggta agggcaatct
     6601 agccttggaa ttttcacagg ccaagggctt agaactagct gtcaccttgt ctgtcaatca
     6661 atggaatggc aataccagtc ttcagctcat gttggttgat gctcgggtca acggtgttca
     6721 actttataat attcgtggaa aacaacatcc tattcctgca ggagtcccaa ttttggacat
     6781 tgaaaatcca gtagcagata acaaagcaat tgtcttggct aacttgcctg aggacctcag
     6841 tagtctgcgt agctattttc aggaaagaga atttgaagcc atttatttta aaaacgaaat
     6901 tgccaaaccg tactatctag atggttatgg tagccgagaa caattcgcta aattgtataa
     6961 gactatttac caatttgatg agtttgatgt tcgctacaaa ctgaaatatt tggctggtta
     7021 cttaaaaatt aaggacagtc tattggttaa aatgattcag attttccaag aattagaatt
     7081 tgtcacgatt acggatgggg tcatgcgggt caacaaagag gcacagaagc gagagatttc
     7141 agaaagtcaa atatatcaag aattgaaaga aacagttatc caacaagagt tgatggcgct
     7201 tggcaccgtt caggaaattt atgaatggct atgtggtcgc gtttgatgac agtagaggct
     7261 ctggttgttg ttggagtgat tggaacgctt ctctacactt attggtttat caggcaaaaa
     7321 ggtttgttga aaaatcttga acagataacc gaaataaagc acgcactgat tgaattacgg
     7381 cgagtaggat ggtcagaaag agccatcaaa aaggttttcc tgaaacaact gttgcctcta
     7441 aaacaagaag acatcccagc atttgttcaa aatttcataa aagaagctac tatttttgcc
     7501 tcaactagct tttatcagtt gataagagtt gacagtcgct ctttaagcca agagaaagcg
     7561 acggctttat tggagcaaag tatgaatatg ttagattttc ctgtggaatt aagtcatggg
     7621 atattacctg aacttttgca gaagatggat gtgaattgtc ctccacacca tccgttttgg
     7681 cggtactttg ctaagttagt tgataaggct tttccagttc gggaattaga agttaaacgg
     7741 ccgttaaaca gacaggtcca tcaactgcgc taccttattt cctatcagca ggacgtcagt
     7801 agtgtggaaa aggaaaaaca gattggcagg ctctgatagc ctatttacgt agcctacctc
     7861 gatggtctta acgcttacga gaatctgccc gcctacacaa taaacaactg ttcggcaaga
     7921 aaaatcagaa gactttgcca gtcaatatga agattttgat tcattttcac agcgaattca
     7981 tcctaaatca agatggacaa tttgccttga ttttagaaga acgacctcat gtcaatggcg
     8041 tggtaaatgg ggcttccttt aattatgcca gagctaataa taagcgggag tgggaaagaa
     8101 ctcgactggt ctaaaaagag ttcgtcaaca agtctttatt tctagttgtt gagctgaaac
     8161 agtctattcc tagactgttt cactcccacc cccgcacagc tcaaactgtc tgggagacag
     8221 tttgaggttg gagataaggc gaactctgtt cgcatcagtc gtagaggtca gatttggagt
     8281 gtaaaacacg aacaaatccg ccaatcaacc actgcgctga gatgttgaca cgaactctaa
     8341 gaagtggtgc tgggcttttt gcccagcctc tccagttggc aggcaggatc ccgtttttcg
     8401 gaaagaattg ctacgctcaa agatgggagt ctatttatcg cctactcgct ttcgtcgcta
     8461 ccaaaaagga agaaatgagg ttggctggga gcagtcttac tttaatcagc aaggaagctt
     8521 ttcctatggt gggcatagca gggcagcttg tgtagcgaat ttgaggagac gatttgctaa
     8581 agatataggt ctgaaatgta ccaaaaaaca atttcatggt ataatgaaga gtagaaatta
     8641 tttttaaaaa gagagagaca aatgaactta aaagattaca tcgcaacaat tcctaactat
     8701 ccaaaagaag gtatcgaatt ccgtgatatt agccctttga tggcagatgg aaatgcttat
     8761 agctatgcaa tacgtgaaat cgttcagtat gcgacagata agcaaattga tatgattgtt
     8821 gggccagagg cgcgtggttt catcgttgga tgtccagttg cctttgaatt gggaatcggt
     8881 tttgcaccag ttcgtaagcc aggtaagtta ccacgtgagg ttatctcagc tgactatgaa
     8941 aaagaatatg gtgtagatac attgacgatg catgcagacg caattaaacc aggacaacgt
     9001 gtcttgattg tcgatgactt gttggcgact ggtggtactg taaaagcaac gatcgaaatg
     9061 attgaaaagc ttggcggtat cgttgctggc tgtgccttct tgattgagct agatgatttg
     9121 aaaggccgcg aagcaatcgg tgattacgac tacaaggttt tgatgcacta ttaatctaat
     9181 gttatagcca aaacttataa ctggtcctat ttttattcta attgaattgc atagcaacct
     9241 atcttataat aaatataaga aaattgggag gttgcttatg ccaattaaaa ttgaaaaatc
     9301 attaccagca gttgaaattt tgagaaaaga gaatattttt gtgatggata gtgaccgagc
     9361 tagtcatcag gacattcgtc ctatcaaaat ccttattctc aatctcatgc cacagaagat
     9421 agcgacggag actcagttgc tccgtctttt ggctaacact cctttacagc tagaggttga
     9481 atttctctac atggctagtc acgagtccaa aaatacacac tcggaccatt tgaagcagtt
     9541 ttataagact tttgagcagg tcagggatag ctattttgat gggctcatcg tgacaggagc
     9601 accagttgaa aacttacctt ttgaggaagt tgattactgg caagaactta ctcaagtttt
     9661 tgattggtcg aaaacacatg tttattcaac attgcacatt tgttggggtg cacaggcagg
     9721 tttgtatgca cgctatggtg tacccaaaca cagtatgtct cgaaaactat caggcgtata
     9781 ttgtcaagaa gtgactaatc caactagccc tcttatgcgt ggatttgacg atgaatttcg
     9841 ttcaccacac tctcgttata cggaagtaag agaagcagat attgcccatc tgagtgggtt
     9901 gacgattttt tcacaaggtc cagaagttgg cctatccatt ttagccagca aggatttaag
     9961 agaaatttat agttttggac atttagagta tgatagggat acactagcta aggaatatca
    10021 acgtgattgt atagctggaa aaaatcccca tttaccggaa aattatttca aagaagatga
    10081 ccctacaagc aaaccatctc tctcttggaa tttaccagca gcacaattct ttaccaactg
    10141 ggtgaattat gctgtctatc aagaaactcc atacgactgg catatctttg aacaatcagc
    10201 ccagtcagct agtctttaaa gaggaagtat gaactattca cagcaatttc gtcagggaca
    10261 tttggtcctg ccagcagcaa tattattcca ctatcaagag ctttttccgt cggcagatga
    10321 ttttttgatt tggcagtttt tcttatatca aaattcttca gctatcgaat cattagcgcc
    10381 tagtgagatt gctcaagcta ctggtaaaac agttgctcag gtcaatcaag ctattgaaaa
    10441 tctccaagat gctggactct tagaatttaa gacaatcagt attgctggtg aaattgaaat
    10501 gatattcgat gcacttcctg cttttgaaaa attggatgtt ctgttaactc caaaacaggc
    10561 agttgagatt gtgcaaccag agaatgattt gaagaccttg gtgggtgatt ttgaacgtga
    10621 attgggccgc tttttatcgc cgtttgaaat cgaagatttg cagaagacta ttgaggatga
    10681 caaaacttcg attgatttgg ttcgtgcagc cttgaaagaa gctgttttta acaacaaaac
    10741 aaattggaaa tacatccagg ctattctacg aaactggcgc cgtgagggaa ttactacagt
    10801 tgcgcaagta gaagcgaaaa atgcagaaag agaaatccag actcctaaga atgtgaccgt
    10861 ttcaagtgac tttcttgatg caatggatct ctggaaagat tgatatggaa acaaaattat
    10921 ctaaaaggct ggaaacagtc gcaagttttg tcccacaagg ggcacgtcta gttgatgtag
    10981 ggagtgacca tgcttatctc cctctttttt tggtagagca ggggcgtatt gattttgcca
    11041 ttgcggggga agtcgttcaa ggtccctatc agtcagcgct ccagaatgta gagcaggctg
    11101 gacaaacaga aaaaatccta gtccgtttgg caaatggctt agccgcgttt gagaagtctg
    11161 accagatgac tgccgtgacc atagcaggta tgggaggacg cttgattgcg gaaatcttag
    11221 aagcaggaaa agaaaagctt gcgaccattg aacgtttgat actccagcca aataatcgtg
    11281 aagatgatgt gcgtcgatgg ttggaaaaaa atggttttag attggttgct gaaaagattc
    11341 tggaagaaaa tggcaagtta tatgaagtcc tcgttgcaga atcaggacag atgagattgt
    11401 cagaagttga acatcgtttt ggtccctatc tactagagga ggcctcacca gttttcgtca
    11461 aaaagtggag aagagaactt gaaaaattaa gttttgcttt ggaacaagtt ccatctgaac
    11521 gccaaggtga ccgctttgcc atttcccaaa agatccaaca aattcaggag gtagtccatg
    11581 ttagctagtc aggttattga gcgctatcaa gccttttgtc ccccttcttt ggctatggaa
    11641 ggcgatatca aagggctgca aatcggtacc cttaataaag agattcaacg tgtcatggtg
    11701 gctctggata ttcgtgagac aacagtggca gaggcgattg ataaaaaggt ggatttgatt
    11761 atcgtcaagc atgctccgat ttttcgtcca ataaaggatt tggtggctga caatccgcaa
    11821 actaaaatgt atcttgattt gattaagcat gatattgcag tctatgttag tcatacagat
    11881 attgatgtgg ttgagggtgg tcttaacgac tggttctgtg aactattaga aattcaagat
    11941 actacctacc tgattgaaac tgcagagggg caggggcttg gccgtgtggg gacaatagca
    12001 gagcagactt tggaagaatt ggctttgaaa gtcaagtctg tctttgatct agatgctgtc
    12061 cgtctagtga tctatgggca ccaagccaag cagtatgtca gccgtgtagc catctgtgga
    12121 ggcagtggtg ggtcttacta ccatgaggct cttgctaagg gggcagatgt ttacatcacg
    12181 ggcgatattt actaccatac tgggcaggac atgctgactc agggtttgct ggctattgat
    12241 ccaggccatc atatcgaagc cttgtttatc aagaaaatta ctgaattgtt ggaaacatgg
    12301 aaggcaaaag aaggttggaa tgtcgaaatt ctaccttcaa ctgtttcaac caatccgttt
    12361 agacatttgt gataaattaa tcagcacttg attgtggaaa gaccaagacg acatttaatg
    12421 tagttttagg tcttttattt tttagatatt ttcttattta tttaagtgaa aaagcttgaa
    12481 ttttataata aattagggta tcttaatttg taggatatgc aagattaaac aggataggat
    12541 gttagttatg acttgggtta ctaatcagtc tgtagttcta caggctttgc taggtggttt
    12601 attcacatgg ttttgtacga ttgtggggtc tgccgttgta tttttcttta agacagttag
    12661 tcgtcgtttg ctagacacca tgttaggctt tgcagctggt gttatgattg cggcttcttt
    12721 ttggtcttta ttagccccat caattgagta tgctgaaagt tcatacggca atctggcttg
    12781 gataccagta gcagtaggtt ttgcagcagg tggtattttt ttacgcttgg tagatgcctg
    12841 ggttccgcat ttgcatttgg gaaatgataa agataaggca gaaggtggtg gtgagaagga
    12901 gagaaaaaat ctatccaaaa cagccctgct cttcttagct attacaattc acaatattcc
    12961 tgaaggattg gcggtcggtg tgacatttgg tgcactagct tctaactata gtccagcagc
    13021 ttttattggc gcaatcggtc ttgctattgg gattggtata caaaatattc ctgaaggggc
    13081 agccttggct attccaattc gtacagacgg cgcatctagg tggaaagctt tttactgggg
    13141 atcaatgtca gcaattgtcg agccgattgc agcagtcatc ggggcttttg cggtcacctt
    13201 tatgacacct attttacctt atgccctctc ctttgcggca ggagccatga tttttgtcgt
    13261 cgtagaagag ctcattccag aatcgcagac aaacggcaat actgatattg caactctggg
    13321 cttaatggct ggttttatca tcatgatgat tttggatgtt gctctaggat aatttgttaa
    13381 agtctgtcgt cacaggcttt tttcttatga caatatcttt aaaaatatgg tataatgaac
    13441 tgatattaat ttagggaagg tactcttata tgaaaggtat tattctagct ggtggatcag
    13501 gtacacgctt gtatcctttg acgcgcgcag cttcaaaaca gttgatgcca atttacgata
    13561 aaccaatgat ttattatcca ttgtcaacat tgatgttggc tggaatcaaa gatattttga
    13621 ttatttcaac tccgcaagat cttccacgtt ttaaggatat gttaggagat ggttctgaac
    13681 ttggaatttc actttcatat gcagaacaac caagtccaga tggtttggcg caagccttca
    13741 ttatcggtga agaatttatc ggtgatgaca atgttgcctt gatccttggt gataacatct
    13801 accacggcaa tggattgacc aagatgttgc aacgtgctgc tagcaaagaa aagggagcaa
    13861 ctgtctttgg ttaccaagta aaagacccag aacgttttgg tgttgttgag tttgacgcag
    13921 atatgaatgc catctctatc gaagaaaaac ctgaagagcc aaaatcaaat tttgctgtta
    13981 ctggtcttta tttctatgac aatgatgttg tggagattgc gaaaaatatc aagccttcac
    14041 ctcgtggcga gttggaaatc acagatgtca acaaggctta cttagaacgc ggcgacttgt
    14101 ctgttgagct tatgggccgt ggatttgctt ggttggatac aggtacgcat gaaagcctct
    14161 tggaagctgc acagtacatc gaaacggttc aacgtttgca aaacgttcaa gtggctaact
    14221 tggaagaaat tgcttaccgc atgggctaca ttacaaaaga acaggtgcat gaattggcgc
    14281 agccacttaa gaaaaatgaa tacgggcaat acttgctccg tttgattgga gaagcttaat
    14341 gacagaaaac tttttcggta aaacactagc cgctcgccca gttgaagcta ttccaggaat
    14401 gttagaattc gacattcctg ttcatggcga caaccgtggc tggttcaagg agaacttcca
    14461 aaaggaaaaa atgctgccac taggcttccc cgaaagcttc ttcgcagaag ggaaattgca
    14521 aaataatgtt tccttctctc gtaaaaacgt tcttcgtggt cttcacgcag aaccttggga
    14581 taaatacatt tcagtagcag acggcggtaa ggttttggga acttgggtgg atcttcgtga
    14641 aggagagacc ttcggtaata cctaccaaac cgttatcgat gcttcaaaag gtatcttcgt
    14701 tccgcgcgga gtagccaatg gtttccaagt cttgtcagat ttcgtggctt acagctactt
    14761 ggttaatgac tactgggctt tggaactcaa gcctaagtat gctttcgtca actacgctga
    14821 ccctagcctc gacatccagt gggaaaacct agaagaagca gaagtttcag aagcagataa
    14881 aaatcaccca ctcctcaaag acgtcaagcc tttgagaaaa gaggacctct aatgtttcaa
    14941 gcctttttgg aaatagcaga gcagttaaac aagttaggaa tcactccctt gttaatgggc
    15001 tcagttggtt tggaaagaca gacaggacga gattggcagg caagggacct tgatattcat
    15061 gtgcccagtg acccacgggg atgggaagcg tcagacgagg agcggattta ccgtgcagat
    15121 gagctgattg ttctgatgaa tcaacttggc tatgtcctgg ttgatcgtca tgaacatgaa
    15181 tttcataaag acggcttatc tgttgaattt ggcggcctac attcactgcc tgaatttgca
    15241 gatgtagagc tagaagattt ggaagaacta gaaacagcag gtgttcgcta ttacctaccg
    15301 acacttgaac aatacctgaa aatctaccaa gcctcttcaa aagattccta ccgagcagaa
    15361 aataacaatc aaaaagactt tgctaagatt gcttacttga aagaaatctt gaagtaaaga
    15421 aagcgagcga agcgagcagt tctgtgtgtc atcgccgtag tgagaagctc ctttgctttg
    15481 ctaaggagcg gggggaacca tgagacctta ggctcatggt ttagtaatga aacaccttgg
    15541 tggttgctta cgaccccact acctaagatg acacacaaaa aagaaaaaaa cttttcggag
    15601 aaaaaaatgt ctcaatttaa aaatattatc gtgactggtg gagctggttt catcggttca
    15661 aactttgtac actatgtata taacaatcat ccagacgttc acgtaactgt tttggataaa
    15721 cttacttatg caggaaacaa ggctaacttg gaagctattc ttggtgaccg tgttgagctg
    15781 gttgttggcg acattgcaga tgttgagttg gttgacaaat tggctgctaa ggcagatgcc
    15841 atcgttcact atgcggcaga aagccacaac gacaactctc tcaacgatcc aagcccattt
    15901 atccatacca actttatcgg tacttacact ttgcttgaag cggctcgtaa atacgacatc
    15961 cgtttccacc atgtatcgac cgacgaagta tacggtgacc ttcctttgcg tgaagatttg
    16021 ccaggtcatg gcgaaggtcc aggcgagaaa tttactgcgg aaaccaacta caacccatca
    16081 tcaccttact catcaaccaa agctgcttca gacttgattg tcaaagcatg ggtgcgttca
    16141 tttggtgtta aagcaactat ctcaaactgt tcaaacaact acggtcctta ccaacacatc
    16201 gagaaattta tcccacgcca aatcaccaat atcttggcag gtattaagcc aaaactttac
    16261 ggtgaaggta aaaatgtccg tgactggatt cataccaacg accactcgac tggtgtttgg
    16321 gcaatcttga caaaaggccg tatgggtgaa acttacctaa tcggtgctga cggtgagaag
    16381 aacaacaaag aagtgcttga attgattctt gaaaaaatgg gtcaaccaaa agatgcttac
    16441 gaccacgtga ctgaccgtgc aggacacgat ttgcgctatg ctattgacgc aagcaaactt
    16501 cgtgacgagc ttggttggac accacaattc acagacttct ctcaaggttt ggaagagact
    16561 atccagtggt atacagataa ccaagactgg tggaaagctg aaaaagaagc tgttgaagcc
    16621 aactatgcta tgacacagga agttattaaa taagattatt aaaggctagg gctcttgccc
    16681 aagcctttct tgaaataagg agcaaacatg attttaatta caggtgcaaa tggtcagttg
    16741 ggtacagaat tgcgttatct cttggatgag cgtggtgttg agtacgttgc cgctgacgta
    16801 gcagaaatgg atatcacgga tgcagataaa gtagatgcct tctttgcaga agtaaagccg
    16861 agcgtcgttt accattgtgc agcctacaca gctgttgata tggcagagga tgaaggcaag
    16921 gagctcaact acaagattaa cgtaacaggt tctgagaatg ttgccaaggc ggcagccaaa
    16981 tacggtgcaa cccttgtcta tatctctaca gactatgttt tcaacggtga tttgccagtc
    17041 ggtcaagagt ggcaagtata tgaccaacca gatcctcaat ctgaatacgg tcgtaccaag
    17101 cgtctgggtg aagaagcagt cgagaaattt gctgacaaat tctacacagt gcgtactgct
    17161 tgggtctttg gtaactacgg taaaaacttt gttttcacca tgcaaaatct tgcggaaaca
    17221 cgcgatactt tgacagttgt caatgaccaa cacggtcgcc caacctggac acgcacactt
    17281 gcggaattca tggtacactt ggtgaacacc aagcaagcat ttggctacta ccatttgtca
    17341 aacgatgcag cagaagatac gacttggttt gactttgcga cggaaatcct caaggatacc
    17401 aacaccaagg tattgccagt ggattctagc cagttcccag ccaaggcaaa acgtccattc
    17461 aactcaacca tgagcttgga caaggcaaag gcgacaggtt ttgtcatccc gacctggcaa
    17521 gaagcattgg aagcatttta taagcaggag aaaaggtagt atttgataac tagaagtggt
    17581 ttatttaagt gaaatgaagc tcttaggcag ttagtctaag ggcttatttt tgaaaaatag
    17641 aagtatactg atttctgtca aaaagacaga tgtctttatt aaaaaataat tttgattctc
    17701 attaaagttt ttaaaaaaat atgc
//
LOCUS       DATUCG010000040        17623 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.041, whole genome shotgun sequence.
ACCESSION   DATUCG010000040 DATUCG010000000
VERSION     DATUCG010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17623
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.041"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(9..875)
                     /gene="sdaAA"
                     /locus_tag="VCA20_001054"
     CDS             complement(9..875)
                     /gene="sdaAA"
                     /locus_tag="VCA20_001054"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEP1834893.1"
                     /translation="MFYTIEELVQQAENFSGNVAELMIATEIELTGRSREEILTIMSR
                     NLTVMKASIVDGLTESKSVSGLTGGDAAKLDAYIKSGKTLSDSAVLSAARNAMAVNEL
                     NAKMGLVCATPTAGSAGCLPAVLGVAIEKLNLTEEQQLDFLFTAGAFGLVIANNASIS
                     GAEGGCQAEVGSASAMSAAALTLAAGGTPYQASQAICFVIKNMLGLICDPVAGLVEVP
                     CVKRNAMGASYAMVAADMALAGIESKIPVDEVINAMYQVGSALPTAFRETAEGGLAAT
                     PTGRRLAQEIFG"
     gene            complement(888..1559)
                     /gene="sdaAB"
                     /locus_tag="VCA20_001055"
     CDS             complement(888..1559)
                     /gene="sdaAB"
                     /locus_tag="VCA20_001055"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEP1834894.1"
                     /translation="MTNLKFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFGEQPT
                     EVEFQLYNSFAKTYRGHGTDVALVAGILGMDTDDPRIPDSLDIARERGIKVYWRVNKE
                     SNTPHPNTTRIIIKNEKKSISATGVSIGGGNIQVTELNGFSVNLNMNTPTIIIVHQDV
                     PGMIAKVTDVLSKYDINIAQMNVTREKAGEKAIMIIEVDARQCENSIAEIEKIPHLHN
                     VTFFN"
     gene            1774..2898
                     /gene="mnmA"
                     /locus_tag="VCA20_001056"
     CDS             1774..2898
                     /gene="mnmA"
                     /locus_tag="VCA20_001056"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEP1834895.1"
                     /translation="MSIDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDD
                     TDENGFCTATEDYKDVAAVADQIGIPYYSVNFEKEYWNRVFEYFLAEYRAGRTPNPDV
                     MCNKEIKFKAFLDYAMNLGADYVATGHYAQVSRDEDGTVHMLRGEDNGKDQTYFLSQL
                     SQEQLQKTMFPLGHLQKPQVREIAERAGLATAKKKDSTGICFIGEKNFKEFLGQYLPA
                     QPGRMMTVDGRDMGEHTGLMYYTIGQRGGLGIGGQIGGDNEPWFVVGKDLSKNILYVG
                     QGFYHESLMSTSLQASQVHFTRDMPEEFTLECTAKFRYRQPDSQVTVKVKGDKAEVIF
                     AEPQRAITPGQAVVFYDGQECLGGGMIDMAYKDGEACQYI"
     gene            complement(2941..3330)
                     /locus_tag="VCA20_001057"
     CDS             complement(2941..3330)
                     /locus_tag="VCA20_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000106206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEP1834896.1"
                     /translation="MSTNLEIFNAYNNALIAGDFAALFETMADDIIWHQPGNHSTSGA
                     KIGKEVLGAHLATFAEKTNGTFKVVTNWVSDNDTLVAANVTFLGTRTDGEELNMNGID
                     LFRMEDGKIKEVWLFSADQKAEDAFWG"
     gene            3456..3782
                     /locus_tag="VCA20_001058"
     CDS             3456..3782
                     /locus_tag="VCA20_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP1834897.1"
                     /translation="MKKVSEFGICPFATTQKVLTGKWGLVIIYQLSTGTKRFNELQRL
                     IPGITQTMLTRHLRQLEEDRIISRTVYAEVPPRVEYSLTEMGEKFRSVLDAVQAWGLE
                     YIETLT"
     gene            3924..4376
                     /locus_tag="VCA20_001059"
     CDS             3924..4376
                     /locus_tag="VCA20_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEP1834898.1"
                     /translation="MDFRTRYDNQIFGVRATGLVVQDGKLYLVKSPEGLYYTLGGAIQ
                     LGETTEEAVQREMKEEIGIDVEVGPLAFIVENQFTLQEKSYHQIEFLYIVTPLSNPAP
                     NLEEGNSIRQCEWVTFADLEKLDLNPAFLKTELANWDGQLKHFMNKDN"
     gene            4393..4836
                     /locus_tag="VCA20_001060"
     CDS             4393..4836
                     /locus_tag="VCA20_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEP1834899.1"
                     /translation="MPVKLIAHVLLTVADSHLIIQRSQIKRGKPNVFPQYWDIPGGRV
                     EENELPRDAAVRECFEETGISIEKENLTIIHEDSQFDEEKQTVFTRLVYEGTLTEQVK
                     TILLDPEEHTDFLWLALSDKNKKNLVPYLDEILEKRRINGFQNQN"
     gene            4814..5275
                     /locus_tag="VCA20_001061"
     CDS             4814..5275
                     /locus_tag="VCA20_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEP1834900.1"
                     /translation="MDFRTRIDNQIFGVRATALIIKDGKIFLTKDSKGRYYTIGGAIA
                     VNETAQDAVVREVKEELGVDSRVNQLAFVVENQFTHEGIDFHNIEFHFIVEPIGEIPE
                     KMIEGQLKQTCEWIELDKLVNLDIVPAFLAQELPNWNGQAKHIMNMKEKTT"
     gene            5272..7188
                     /gene="mnmG"
                     /locus_tag="VCA20_001062"
     CDS             5272..7188
                     /gene="mnmG"
                     /locus_tag="VCA20_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEP1834901.1"
                     /translation="MTHTFAENYDVIVIGAGHAGVEAGLAASRMGCKTLLATINLDMV
                     AFMPCNPSIGGSAKGIVVREIDALGGEMGRNIDKTYIQMKMLNMGKGPAVRALRAQAD
                     KAEYAAEMKRTVERQENLTLRQTMIDEILVEEGKVIGVRTATNQKFSAKAVVVTTGTA
                     LRGEIIIGDLKYSSGPNNSLASITLADNLKELGLEIGRFKTGTPPRVNARTINYEETE
                     IQPGDEKPNHFSFLSKDEDYLQDQIPCWLTYTNATSHEIINSNLHRAPMFSGIVKGIG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRNTDEIYVQGLSTSLPEDVQQDLIHSIKGL
                     ENAQMMRTGYAIEYDMVMPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIVAGI
                     NAALKVQGKPELILKRSDGYIGVMIDDLVTKGTVEPYRLLTSRAEYRLILRHDNADMR
                     LTEIGRQVGLVDDERWQVFQIHKNQFDNEMKRLESIKLKPIKETNEKVVAMGFKPLTD
                     ALTAKEFMRRPDVTYADAVAFIGPAAEDLDAKTIELIETEVKYEGYIAKALDQVEKMK
                     RMEEKRIPADIDWDDIDSIATEARQKFKLISPETIGQASRISGVNPADISILMVYLEG
                     RSRSISKNKSKDSH"
     gene            7295..9256
                     /locus_tag="VCA20_001063"
     CDS             7295..9256
                     /locus_tag="VCA20_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEP1834902.1"
                     /translation="MKRFRFSTIHFVMIGVILFGLVALVHRLFPIGASTVFALLISLL
                     VLIALFIYQKHSYEFSELEQIEYLNNQANSGLMMLLDKMPVGVIKVRPDSNQVEWFNP
                     FAELIFAQENGEFDQKKLREIIDVGLDEERIYANFSGKRYAVNVDFDQGLFYFFDAST
                     EYSATNSLIGSRPVIGIISVDNYDELEDAVTDSQISQVNSFLAQFVSEFCHDYGIYYR
                     RGTMDRFYFFTDYAVLEKLIENKFSVIDKFREEAKKLDLGLTLSMGLAYGNENHHQIG
                     QVALQNLNMAEVRGGDQVVVKENDESKQPLFFGGGSASSVKRTRTRTRAMMTAVSDKL
                     KSVDAVFVVGHRNLDMDALGSAVGMQYFAQNIMNNAYTVYNPDEMAPDIARAIKKLSD
                     ENCSNLLTVEEAMKMVTFQSLLIMVDHSKIGLTLDKDFYEQFTQVVVVDHHRRDTDFP
                     NNAVLTYIESGASSASELVTELIQFQNDKHHKLNRIQSSLLMAGIMLDTKNFTSRVTS
                     RTFDVASYLRTRGSDSLEIQQIAATDFEEYRQINELILSGQRIESNVVIACADDTKEY
                     DNIIPSKAANTILDMAGIEAVFVITRNTKGYVSISARSHSKINVQRIMEEMGGGGHFN
                     LAAAQVYDQTIAEVCEKLTDKIREEINGN"
     gene            9267..9719
                     /gene="rplI"
                     /locus_tag="VCA20_001064"
     CDS             9267..9719
                     /gene="rplI"
                     /locus_tag="VCA20_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEP1834903.1"
                     /translation="MKVIFLADVKGQGKKGEIKEVPTGYAQNFLIKKNLAKEATNQAI
                     SALRGQEKSKEKAHAEMIAEAEAIKAKLAEEATLVKFVEKVGPDGRTFGSITSKKIAE
                     ELDKQFGIKIDKRHIQLDHPIRAIGLIDVPVKIYQDVTGIINLSIKEA"
     gene            9751..11106
                     /gene="dnaB"
                     /locus_tag="VCA20_001065"
     CDS             9751..11106
                     /gene="dnaB"
                     /locus_tag="VCA20_001065"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEP1834904.1"
                     /translation="MAELDELRVQPQDILAEQSVLGAIFLDEGKLVFVREYIEAEDFF
                     KHAHKLIFKAMIALSDRNEAIDATTMRNYLVNHGDLENIGGLGYLAEIISSVPTAANA
                     EFYAKIVAEKSNLRKLIARLTDAVNQAYEGADGSDDIIANAEKALIDVSEGASRSGFK
                     RIDDVLNINFDNLETRAKQTSDITGIATGYPALDAMTTGLHEEELIILAARPAVGKTA
                     FALNIAQNIGTKLGLTVAIFSLEMGAESLVDRMLASEGVINSRAIRTGHLTQEEWNKY
                     MVAQANLARASIYIDDTPGIKITEIRSRSRKLAQETGNLGLILIDYLQLITGTGRENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYERGEQEDGGIPNNTVEVIIEKNRSGARGTVELMFQKEYNKFASISKRED
                     G"
     gene            11110..11361
                     /locus_tag="VCA20_001066"
     CDS             11110..11361
                     /locus_tag="VCA20_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834905.1"
                     /translation="MSDAFTDVAKMKQIKQDIKDHEGQIVELTLENGRKREKNKQGRI
                     VEVYQSLFIVEFEDPNNTFVESYTYSDILTEKILIHYLD"
     gene            11502..12197
                     /locus_tag="VCA20_001067"
     CDS             11502..12197
                     /locus_tag="VCA20_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEP1834906.1"
                     /translation="MEFQWFTVLALFAEGLLSFLSPCVLPILPIYVGLLAGGAEGKEG
                     EKKVLVNTLSFVVGIALTFFLLGFASSLLSRVLQANSQVLQIISGLLIVLMGILQLGW
                     FNIPMLEREFSAKNKVYKAGKNVTPFLAFLMGFTFSFSWTPCIGPILASVFIYASSQQ
                     GWLSSILLLVYCLGFILPFILVAFFSQKMLAFFKNNRHYLAWTKRISGYLLLLIGLSI
                     LTGFFQQFVRFFH"
     gene            12216..12779
                     /locus_tag="VCA20_001068"
     CDS             12216..12779
                     /locus_tag="VCA20_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEP1834907.1"
                     /translation="MKKLVSIVVCLLVSCFVLTACSLLPLVSPSSTESTLQANQPVTL
                     DFSLKDKDGKDVNLADYKGKKIYINVWATWCGPCQREIPELEEVYKEYKDKEDYVFLS
                     ITSPKDDEFANTNPSDVSKDEILEKASELGATYPVLFDTKDQAMTHFGIVAFPTHILI
                     NSDGTLKVSFAGQVQKDKLLELLEEMQ"
     gene            12986..13597
                     /gene="rpsD"
                     /locus_tag="VCA20_001069"
     CDS             12986..13597
                     /gene="rpsD"
                     /locus_tag="VCA20_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEP1834908.1"
                     /translation="MSRYTGPSWKQARRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFSYGLGEKQFRNLFVQATKIKQGTLGFNFMLLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVEVGQVISVREKSIKVPAILEAVEATL
                     GRPAFVSFDTEKLEGTLTRLPERDEINPEINEALVVEFYNKML"
     gene            13852..15387
                     /locus_tag="VCA20_001070"
     CDS             13852..15387
                     /locus_tag="VCA20_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEP1834909.1"
                     /translation="MDTRINEAMQKFSRSVIVPVKFMAVMGLFLAIGVILQLQFMPEF
                     IQTLGSLIKTMMDSMLNNLSLIFCIGIASSLANKKKVEASLIALIAFLFFLAANNAWL
                     TLTNQLASAGEVGLFGTGQGMVLGFQVVDMNVFLGMMIGCLVGWFHNKFSEKEFIDLF
                     SIYGGSRFTFTLLIPVILVLAIAMCYIWPVVNQGISSLSGFILTTGLVGVFIYAFGNR
                     FLIPTGLHHLLWMPFCFTALGGTAEINGKIYSGAVNIFYAELANSGSIVSLDNSLRFA
                     TFGFVKIFGSIAVAAAIIYCARKEKRKEVEGMMLPSTFVAMAAGITEPLDFTFLFLSP
                     LLWFIHSLLTAISETILWALGARTYMLYGLIDTVVSNAPFSPNITKFYLVIIVGIIMT
                     IVWFVTFVFLIKKLDVKTPGREDIVEPVSETVASIVETMDDTALVIKGLGGADNIEHV
                     TNCFTRLRVTVKDEMKVDDHILQKVSQQKGVVKNGKNIQVIIGMGVQTFKEEVCATLG
                     ITE"
     gene            15402..16739
                     /locus_tag="VCA20_001071"
     CDS             15402..16739
                     /locus_tag="VCA20_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018373952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HEP1834910.1"
                     /translation="MVREKQSVVIAGGGSTYTAGIVMMLINNQDRFPLRKLKFYDNDK
                     ERQDIVAKACEVIVRERAPEIEFVATTDPEEAFSDVDFVMAHIRVGGLKMREQDEKIP
                     LKYGVVGQETCGPGGIAYGMRSIKGVIELIDYMEKYAPNAWMLNYSNPAAIVAEATRR
                     MRPESKIINICDMPVAIKKSMADILGLKNENEIIDRYYGLNHFGWWTEMYDKEGNDLM
                     PILRKHVQKYGYAAATDEHNPLLEEASWKDTFLKVKDVQAVDQATLPNTYLKYYLYPD
                     YVVAHSNPDYTRANEVMDHREKNVFAECKRIADHQTAADTSIEAGEHAEFIVQLAGAL
                     AYNTYERMLLIVKNEGAIANISEDAMVEVPCIVSKRGYEPLCMGSIPHFQKGMIEQQV
                     AVEKLVVDAYQQESYEKLWQALSLSKTVPSATVAKQILDELIEANKEYWPTLA"
     gene            16812..17579
                     /locus_tag="VCA20_001072"
     CDS             16812..17579
                     /locus_tag="VCA20_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018373953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEP1834911.1"
                     /translation="MRFTDIVNEHYSELNSSELLMCNYIIENAATIPKMGVVEFARRS
                     LSSKSSVIRFSQKLGFTGFTELKNFIKWQDQEEVLIEQLTFKEQVTKDIHSLLELIAK
                     SDWSPIYQRLDTIDNIYVITTGVAQKNQAAELQRLFLLIGKPLQIVPGDGNSNEFRRI
                     IERLTAKDIVFVLSLSGENNNLESIVKRLKLVKSNIVSITSLQNNWLSGKSDFNLYAT
                     TSRSPLPRDWWLQTTSSFFVLIEAFTFGYVDYKKRIK"
BASE COUNT         5154 a         3185 c         4117 g         5167 t
ORIGIN      
        1 tgtcaatctt atccaaaaat ttcttgagca aggcgacgac ccgtcggtgt agctgcaagg
       61 cctccttcgg cagtttcacg gaaggcagtt ggtagggcag atcccacttg gtacatagcg
      121 ttgataactt catcaacggg gattttggat tcgatacctg ctagtgccat atcggcagct
      181 accatggcgt agcttgctcc cattgcatta cgtttgacac aagggacttc taccagacca
      241 gcgacgggat cgcaaatcag acccagcata tttttgatga caaagcagat ggcttggctg
      301 gcttggtagg gagttccacc tgctgccagt gtcagggctg cggcagacat agcagaggca
      361 gatccgactt ctgcctgaca gcctccttct gcacctgaga tggaggcgtt gttggcaatg
      421 accagaccaa aggctcctgc tgtaaagagg aagtcaagtt gctgttcttc tgtcaaattg
      481 agcttttcaa tagcaactcc tagcacagct ggtagacaac ctgcagatcc tgctgtcgga
      541 gtggcacaga ctaagcccat tttggcattc aattcattaa cggccatggc atttcttgcg
      601 gcggatagga cagcagaatc tgacagggtt ttaccagatt ttatataggc atctaacttg
      661 gcagcatcgc ctcctgttaa accagatacg gatttgcttt cagtaagtcc atctacgatg
      721 gaggctttca taactgtcaa gtttcttgac ataattgtca atatttcttc gcggctacga
      781 ccggtcaact caatttcagt cgcgatcatg agttcagcta cattaccaga aaagttttct
      841 gcttgctgaa ctaattcttc gatagtataa aacatgtctt tctctttcta gttgaagaag
      901 gttacattgt gcaggtgtgg aattttttca atttctgcaa tagaattttc acattggcga
      961 gcatccactt cgataatcat aatggctttt tcgccagctt tttcacgtgt cacgttcatt
     1021 tgagcgatat tgatgtcata cttagaaaga acatcggtca ctttcgcaat cataccaggg
     1081 acatcttggt gaacaataat aatagttggc gtattcatat tcagattgac agaaaatcca
     1141 tttagttccg ttacctgaat atttcctcct ccaatggata cacctgttgc agaaatagat
     1201 tttttctcat tttttattat gattcgcgtt gtattgggat ggggtgtatt actctctttg
     1261 ttgacacgcc agtaaacttt gataccacgt tccctcgcaa tatctaaaga gtcagggatc
     1321 cgtggatcat ctgtgtccat acctaaaata cctgcgacca aggctacgtc agtaccatga
     1381 cctcggtatg ttttagcgaa ggagttgtag agttggaatt caacttcggt gggttgctct
     1441 ccaaagatag atgagacaat tttcccaatt cggaccgcac cagctgtgtg actagaagat
     1501 ggaccaatca taacaggtcc aataatatca aagaccgact gaaattttaa atttgtcata
     1561 gtaatcctca cgtttttctt ggtactatta tatcaaattt tccagttaga gaatatgcct
     1621 agatgaatgt gttttcttat gttaaaattc tatcgcttac cctttttgta tggtataata
     1681 caagttgttc aaaatcgtca atcgtttttg gataaccaaa ttttggaata cgaccttcaa
     1741 atcgttattt ctacgaaaag atgggagaag attatgtcaa ttgacaattc taaaacccgt
     1801 gttgttgtcg gtatgagtgg cggtgtagat tcatcggtta cggctctctt gctcaaggag
     1861 cagggctacg atgtgattgg catcttcatg aaaaactggg acgacacgga tgaaaatggc
     1921 ttctgtacag caacagaaga ctacaaggat gtggctgcag tggcagacca aatcggcatt
     1981 ccttactact ctgtcaactt tgaaaaagag tactggaacc gcgtatttga gtacttttta
     2041 gcagagtatc gggctggccg cacgcccaat ccagatgtca tgtgtaacaa ggaaatcaag
     2101 ttcaaggcct tcttggatta cgctatgaac ttgggtgcgg actatgtggc gacggggcat
     2161 tatgctcagg tgtcacgcga cgaggacggc actgtccata tgctacgtgg ggaagataat
     2221 ggtaaggacc agacctattt ccttagccaa ctctcacagg aacagctgca gaaaaccatg
     2281 tttccgctcg gccatttaca aaagcctcag gtgagggaaa tagcagagcg agcaggcttg
     2341 gcgacagcta aaaagaaaga ctcgacaggc atctgcttta tcggtgaaaa gaacttcaaa
     2401 gaatttttag ggcagtattt gccagcccag cccggtcgga tgatgaccgt tgacggtcgt
     2461 gacatgggag agcatacagg cctcatgtac tatacaatcg gtcagcgggg cgggcttggt
     2521 atcggaggac aaattggtgg ggacaatgag ccttggtttg ttgtcggaaa agacctgtca
     2581 aaaaatattc tctatgtcgg tcaaggcttc tatcatgagt ccttgatgtc aacttcttta
     2641 caagctagtc aagtccactt tacacgtgat atgcctgaag aattcacact tgagtgtaca
     2701 gctaagttcc gctaccgtca accagacagt caagtaactg tcaaggtaaa aggtgataag
     2761 gcagaagtca tctttgcaga gccacaacgg gctatcacac caggacaagc cgtagttttc
     2821 tacgacggac aagaatgctt gggcggaggt atgatcgaca tggcctataa agatggagag
     2881 gcttgtcagt atatttaatc aaaaataaaa gcttagcacc ctcgtgctga gctttttatc
     2941 ttatccccag aaagcatctt cggctttttg gtcggctgag aagagccaaa cttccttgat
     3001 tttgccatct tccatacgga aaagatcgat accattcatg tttagttctt caccatctgt
     3061 tctagtgccg aggaatgtta cgttagcggc aacaagagta tcattatctg acacccagtt
     3121 tgtcactact ttgaaagttc catttgtttt ttcggcgaaa gttgctagat gtgctccgag
     3181 tacttcttta ccaattttag caccagatgt tgaatgattt ccgggttgat gccagataat
     3241 gtcatctgcc atagtttcaa aaagagctgc aaaatcacca gcgatgagag cattgttata
     3301 ggcattaaaa atttccaagt ttgttgacat ttgatgtcct ccaatttttt tgatatagta
     3361 caattatagt ggaagtagtc aattacgcaa gtaggtactt ttgcatttgt tggtatcaaa
     3421 agtgatacta ttttgataaa taaaggagaa tatttttgaa aaaagttagt gaatttggta
     3481 tttgcccttt tgcaacaaca caaaaagtct tgactggtaa gtggggattg gtgattatct
     3541 accaactcag tacaggaacg aaacgcttca atgaattaca gcgactgatt ccagggatta
     3601 cccaaaccat gttaacaagg caccttagac aattggagga ggatcgcatt attagtcgta
     3661 cggtctatgc agaagttccc cctcgtgtag agtacagctt gaccgaaatg ggcgaaaaat
     3721 ttcgttcggt actggatgct gttcaggctt ggggcttaga atatattgaa acgttaacgt
     3781 aacaccagat ttgaaattct acaaatcttt ggatagaata agagtaacaa ttatcatgat
     3841 ggtcaaagct catgggaatt gtaggctttt ttgtcctgca attttcgaga gttttggcta
     3901 ttttttgcga ttggggagag aagatggatt tcagaacgag atacgacaat caaatcttcg
     3961 gtgttcgggc aacgggcttg gtggtacaag atgggaaact ttatctcgtt aagtcacctg
     4021 aagggctata ctacactttg ggtggagcta ttcagcttgg cgaaacaacc gaagaggctg
     4081 tgcaacggga aatgaaagaa gaaatcggaa ttgatgtgga ggttggacca ctggctttta
     4141 tcgtcgaaaa tcagttcacc ttacaggaga aatcctatca tcagattgaa tttctctata
     4201 tagttacccc actgtctaat ccagctccca atctggaaga aggaaattct attcgtcagt
     4261 gtgaatgggt tacttttgcg gacttagaga aactggacct caatccagcc tttcttaaga
     4321 ccgagttagc caactgggac ggacaactaa agcattttat gaataaagac aactaaagtg
     4381 gcggaggaaa ccatgcctgt taaattgatt gcccatgtct tgctgactgt tgcagacagc
     4441 cacttgatta ttcagcgttc gcagattaag cgtggcaaac ccaatgtttt tcctcaatat
     4501 tgggacattc caggcggtcg tgtcgaggag aatgagttgc cccgtgatgc tgcagtgaga
     4561 gagtgttttg aagaaacagg tatttcgata gaaaaggaaa atctgaccat tatccatgaa
     4621 gatagtcagt ttgatgaaga aaaacagacg gtgtttacaa ggctggtcta tgaaggaaca
     4681 cttacagaac aggtgaaaac cattcttctt gacccagaag aacatacaga ttttctatgg
     4741 ttagccctta gcgataaaaa taagaaaaac ttagttccat atctggacga aattttagaa
     4801 aagagaagga taaatggatt tcagaaccag aattgacaat cagattttcg gtgttcgggc
     4861 gactgcattg attataaaag acgggaaaat atttctcact aaagactcta aggggcgtta
     4921 ttataccatt ggcggtgcga ttgcagtcaa cgaaactgcg caagatgcag ttgttcgaga
     4981 agtcaaagaa gaattagggg ttgacagtcg tgtcaaccaa ctggcttttg ttgtcgaaaa
     5041 tcaatttaca catgaaggca tagactttca caacatcgag tttcatttta tcgtggagcc
     5101 tattggagaa atccctgaga agatgattga aggccaatta aaacaaacgt gtgaatggat
     5161 tgaacttgac aaacttgtca acctagatat cgtgccagcc tttctggctc aagaattgcc
     5221 aaactggaat gggcaagcca aacacatcat gaatatgaag gagaaaacaa catgacacac
     5281 acatttgcag aaaactacga tgtcatcgtg atcggagcgg ggcatgcggg tgtagaagca
     5341 ggattggcag ccagccgcat gggctgtaag accttgcttg caactattaa cttggatatg
     5401 gtggccttta tgccttgtaa tccctccatt ggtggctctg ccaaggggat tgtggtaaga
     5461 gaaattgatg ccttgggtgg cgaaatgggt cgcaacattg acaagaccta tatccagatg
     5521 aagatgctca atatgggcaa gggaccggct gttcgtgcct tgcgggctca ggcagataag
     5581 gcagagtacg cagcagagat gaagcggacg gtggagcgtc aggaaaatct gaccctgcgt
     5641 caaaccatga ttgatgagat tttagtggaa gagggcaagg tgatcggtgt ccgcacggct
     5701 accaaccaga aattttcagc caaggcagtt gtggtgacga cgggtacagc cttgcgtggt
     5761 gagattatca tcggtgacct caagtattcg tcaggtccta acaatagcct agcttctatc
     5821 acgctggcgg ataatctgaa agaattggga cttgaaattg gtcgtttcaa gacagggaca
     5881 ccaccgcgtg tcaatgctcg taccattaac tacgaagaaa ccgagattca gccaggcgat
     5941 gaaaagccaa accacttttc attcttgtcc aaggacgagg actatttgca ggatcaaatt
     6001 ccttgctggt tgacctatac caacgcgacc agccatgaaa ttatcaacag caatcttcac
     6061 cgggcaccta tgttttcagg gattgttaag gggattggtc cccgttattg tccgtctatt
     6121 gaggacaaga ttgttcgttt tgcggataag gaacgccacc agcttttctt agagccagaa
     6181 ggtcgcaata ccgatgaaat ctatgttcaa gggctgtcaa ccagtctgcc agaagatgtg
     6241 cagcaggact tgatccactc tattaaaggt ttggaaaatg cccaaatgat gcggacaggc
     6301 tatgccattg agtacgatat ggttatgcct catcagttgc gggcaacgct tgaaaccaag
     6361 aagatttcag ggctctttac agcaggtcag accaacggaa catcaggtta tgaagaagca
     6421 gcaggtcagg gaattgtcgc tggtatcaat gcagccctca aggtgcaggg caagcctgag
     6481 ctgattttga aacgaagcga cggttatatc ggcgttatga ttgatgacct ggtaaccaag
     6541 ggaacggtgg agccttaccg cctcttgacc agtcgagcag aataccgctt gattctgcgt
     6601 catgacaatg ccgatatgcg tctgacggag attggtcgtc aggttggctt ggtagatgat
     6661 gaacgctggc aggtcttcca gattcacaaa aaccaatttg acaatgaaat gaaacgcttg
     6721 gagtctatca aactcaagcc aatcaaggaa accaacgaaa aagtcgtagc tatgggcttc
     6781 aagcccttga cagacgctct gactgccaag gaattcatgc gtcgtccaga tgtgacttac
     6841 gcagatgcgg tagcctttat cggtcctgcg gcagaggatt tggacgccaa gaccattgaa
     6901 ttgattgaaa cagaagtcaa gtacgagggc tacattgcca aggccttaga tcaggtggag
     6961 aagatgaaac gtatggaaga aaaacgcatt ccagccgaca ttgactggga tgatattgac
     7021 tccattgcga ccgaggctcg tcagaaattc aagctaattt caccagaaac cattggccag
     7081 gctagtcgga tttctggtgt caatccagcg gatatatcca ttctcatggt ctacttggaa
     7141 ggacggagcc gttccatttc taaaaacaaa tcaaaggaca gtcactaagg ctgttctttt
     7201 cgctgtatgt cagctatatg tcaactactt taaaatcatg tgaaaagtag cttttttact
     7261 gaatttatgg tagaatggca ggcagaggtt agtgatgaaa agatttcgat tttcgacaat
     7321 tcactttgtc atgataggtg tcattttatt tgggttggtg gctcttgtcc accgactttt
     7381 tccaattggt gctagtacag tttttgcgct actcatcagc ctattggtgt tgattgcgct
     7441 gtttatttat caaaaacatt cttatgaatt tagtgagcta gaacagattg aatatctcaa
     7501 taatcaggcc aattctggtt tgatgatgct cttggataaa atgccagtgg gagttatcaa
     7561 agttcgacca gatagtaatc aggtagaatg gtttaatccc tttgctgagc tgatttttgc
     7621 tcaagaaaat ggtgagtttg atcaaaaaaa actacgtgaa attattgacg ttggtctgga
     7681 tgaggagcga atttatgcaa acttttcagg caagagatac gctgttaacg ttgattttga
     7741 tcaagggtta ttttactttt ttgatgcgtc gacagagtat tcagcgacaa atagtttgat
     7801 cggaagccgt cctgttatag ggattatttc agttgataac tatgatgagc ttgaggatgc
     7861 tgtaactgat tctcaaatta gccaagtcaa ttccttcttg gctcaatttg tttcggaatt
     7921 ttgccatgat tacggtatct actaccgacg cggcacaatg gatcgattct atttctttac
     7981 agattatgct gtgttggaaa aattgataga gaataagttt tcagttattg ataagttccg
     8041 cgaggaggct aaaaagttag atttaggttt gactttgagt atgggattgg cgtatggtaa
     8101 tgaaaatcat caccaaatcg gacaggttgc gctccaaaat ctcaatatgg ctgaggttcg
     8161 tggtggagac caggttgtgg tcaaggaaaa tgatgagagt aaacagccac ttttctttgg
     8221 cggtggctcg gcttcttccg ttaaacgaac acgtacgcgg actcgtgcta tgatgactgc
     8281 tgtatcagat aaactcaaat cggttgatgc tgtttttgtg gtgggacatc gtaatttaga
     8341 tatggacgcg cttggatccg cggtcggtat gcagtacttt gctcaaaata ttatgaacaa
     8401 tgcctatacg gtgtataatc cagatgaaat ggcaccagat attgcacgtg ccatcaagaa
     8461 gttgagtgat gaaaattgtt ctaatttgct aacggttgag gaagccatga agatggtcac
     8521 tttccagtcg ttgttaatca tggtagatca ttcaaagatt ggcttgactt tggataagga
     8581 tttctatgaa caatttaccc aggttgtggt ggttgatcat catcgtcgcg atacagattt
     8641 cccaaataat gcagttttga cttatataga gagtggtgct agttctgcga gtgagttagt
     8701 aacggaatta attcagttcc agaatgataa acaccataag cttaatcgca ttcaatctag
     8761 tttattgatg gctgggatta tgcttgatac aaaaaatttc acctctcgtg tgactagtcg
     8821 tacttttgat gtggctagtt acttgcggac tcgtggtagc gatagcttgg aaatccagca
     8881 gattgctgcg acagattttg aggaatatcg tcaaatcaat gaactgattt taagtgggca
     8941 gagaattgag tccaatgttg ttattgcgtg tgcagatgat actaaagaat acgataatat
     9001 tattccgagt aaggctgcta atacaatatt ggacatggca ggaattgagg cggtattcgt
     9061 tataacaaga aataccaaag ggtatgtatc tatttctgca aggagtcata gtaaaatcaa
     9121 tgttcaacgc atcatggaag aaatgggtgg aggtggccac tttaacctag ctgccgctca
     9181 ggtatatgac cagacaatcg cagaagtatg tgaaaagttg acggataaaa tccgcgaaga
     9241 aataaatgga aattaggaga acagatatga aagtaatctt tttagcagat gttaaaggtc
     9301 aaggtaaaaa gggtgagatt aaggaagttc caacaggtta cgcccaaaat ttcttgatca
     9361 agaaaaattt ggctaaggaa gcaacaaacc aagcaatcag cgcccttcgt ggtcaggaaa
     9421 aatctaagga aaaggctcat gcagagatga ttgcagaagc agaggctatc aaggctaagt
     9481 tggctgagga agctacttta gtgaaatttg tcgaaaaagt tggtccagat ggtcgtacct
     9541 ttggttctat taccagcaag aaaattgctg aagaattgga caagcaattt ggcatcaaga
     9601 ttgataaacg ccatattcag ctggatcacc caattcgtgc gattggtctc attgatgtac
     9661 cagttaaaat ctatcaagat gtaacaggca tcattaattt gagtattaag gaagcttaat
     9721 tatttatcta gccagtgagg aggtgagatc ttggcagagt tggatgaatt gagggtgcaa
     9781 ccgcaggata ttctggctga acaatcggta ttaggagcta ttttccttga tgaaggcaag
     9841 ctggtcttcg ttcgtgaata catcgaagct gaagattttt tcaaacatgc tcataagcta
     9901 atttttaaag ctatgattgc tctttctgat agaaatgagg caattgatgc gactaccatg
     9961 cgtaactatt tagtgaacca tggtgatttg gaaaatatcg gtggtttggg ctatttggct
    10021 gaaatcatta gttccgtacc gacggcagcc aatgcggaat tctatgccaa gattgttgct
    10081 gaaaagtcta atctccgtaa actgattgcc cgtttgacgg atgcagttaa tcaggcttat
    10141 gagggtgcgg atggttcaga tgacattatt gccaatgctg aaaaagcttt gattgatgtc
    10201 agtgagggag ccagtcgaag tggtttcaag cggattgatg atgtcttgaa tatcaacttt
    10261 gacaaccttg aaactcgtgc taagcagacg tcggatatta cagggattgc aactggctat
    10321 cctgctcttg atgccatgac aacgggtctg catgaggaag agttgattat tcttgcggct
    10381 cgtcctgcgg ttggtaagac ggcatttgct ctcaatattg ctcagaatat tggtacaaaa
    10441 ctgggtttga cagttgccat cttttctttg gagatgggtg cagaaagctt ggttgaccgt
    10501 atgctcgctt ctgagggagt tatcaattcg agggctattc gtacggggca tttgacgcag
    10561 gaagaatgga ataagtacat ggtggctcaa gctaacctgg ctcgggctag catttacatt
    10621 gatgatacac caggtatcaa gattacagag attcgttcgc gttcacggaa gttggctcag
    10681 gaaacgggca atctgggctt gatcttgatt gactatcttc aattgattac gggaacaggt
    10741 cgtgaaaacc gacaacaaga ggtttcagag atttcccgtc agttgaagat tttggccaag
    10801 gaattgaaag ttcctgttat cgccctcagt cagttgtcgc gtggtgtgga gcaacgtcag
    10861 gataagagac ctgttctatc tgatattcgt gaatcgggat caatcgagca ggatgcggat
    10921 atcgttgcct tcctgtatcg tgatgattat tatgaacgag gtgagcaaga agatggtggc
    10981 attccgaata atacagttga agttatcatc gagaaaaacc gttccggtgc gcgtggtacc
    11041 gtggaactga tgttccaaaa agaatacaat aaatttgcaa gtatttccaa gagggaggat
    11101 ggatagagaa tgagtgatgc atttacagat gttgctaaaa tgaagcaaat caaacaagat
    11161 attaaggacc atgaggggca gattgtcgag ttgacccttg aaaatggtcg taagcgtgag
    11221 aagaataagc aggggcgtat tgtggaggtt tatcagtctc tctttatcgt agagtttgaa
    11281 gacccaaaca atacttttgt agaatcctat acttactcag acattttgac ggaaaagatt
    11341 ttgattcatt acttggatta ggaatggaag cctgtgattg caggcttttt tgtatgattt
    11401 aacaaaacga tatattgact aaggtaaagc ggtgtgttac aatattagta cataaaaacg
    11461 ttttcaatac tgtcttttga gctagataga aaggagttta tatggaattt caatggttta
    11521 cagttctggc tctgtttgca gaaggactct tgtctttctt atcaccctgt gtactgccga
    11581 ttttacccat ctatgttggt ttattagcag gtggagctga ggggaaagag ggagagaaga
    11641 aggttctcgt caatacgcta agttttgtag ttgggattgc tctgaccttc ttcttattgg
    11701 gatttgcctc tagtttgctg agtagggtct tacaagcaaa ttctcaagtc ttgcagatta
    11761 ttagtggtct attgattgtt ctgatgggaa ttttgcagtt gggttggttc aatattccta
    11821 tgttagagcg tgaattttct gctaaaaata aggtgtacaa agcagggaaa aatgtcacac
    11881 catttctagc tttcctgatg ggttttacct ttagtttttc ttggactcca tgtattggac
    11941 ctattctagc atctgttttt atttatgcta gcagtcagca gggttggttg agtagtatct
    12001 tgctgttggt ctattgtctt ggctttattc ttccctttat tttggttgcc ttcttttctc
    12061 agaaaatgtt ggctttcttc aagaacaatc ggcactattt ggcttggact aagcgtattt
    12121 caggctattt attgctcttg ataggcttga gtattttaac aggtttcttc cagcaatttg
    12181 tgcgtttttt ccactaggat agaaaggatt tgtttatgaa aaagttggta tcgattgtcg
    12241 tttgcttact tgtttcttgc tttgttttga ctgcctgctc ccttttacca ttggtcagtc
    12301 catcttctac tgagtcgact ttacaggcaa atcaaccagt cacgctagat ttttccctaa
    12361 aggataagga tgggaaggat gtcaatctag cagattataa gggaaaaaag atttacatca
    12421 atgtttgggc gacctggtgt ggtccttgtc agagggaaat tcctgaattg gaggaagttt
    12481 acaaagagta caaggataag gaagattatg ttttcttgtc tatcacgtca cctaaggatg
    12541 acgagtttgc gaataccaat ccatccgatg tgagcaagga tgagatcttg gagaaggcta
    12601 gtgagttagg agcgacctat ccagtcttat ttgataccaa ggatcaggcg atgacacatt
    12661 ttggtattgt tgcttttccg acacatatcc ttatcaatag cgatggaacc ctcaaggttt
    12721 cttttgcagg acaggttcaa aaggataagc tgcttgaatt attggaagaa atgcaataag
    12781 aacgatgaaa aaggctttac agagcctttt ttttgtgcta tactaatctt tgtgcgaaat
    12841 aacagcaggg caaaatgaag ctcgtcaaca gaaggtcagc aagaagagta atcgttgctg
    12901 tttcgatgaa ggcctcctgc acgaatcagg tttgcttaaa acgttatttc ctacagaata
    12961 cttatttttt aggaggacat aacaaatgtc acgttataca ggaccatctt ggaaacaagc
    13021 tcgtcgtctt ggcttgtcat tgacaggtac aggtaaagaa ttggcacgtc gtaactacgt
    13081 accaggtcaa cacggaccaa acaaccgttc taaattgtca gaatacggtt tgcaattggc
    13141 tgagaaacaa aaattgcgtt tctcttacgg tttgggtgaa aaacaattcc gtaacttgtt
    13201 cgtacaagct actaaaatca aacaaggtac tcttggtttc aacttcatgc ttcttttgga
    13261 gcgtcgtttg gataacgtag tttaccgtct tggtttggca actactcgtc gtcaagcacg
    13321 tcaattcgta aaccacggtc acatccttgt tgacggcaaa cgcgttgata ttccttcata
    13381 ccgcgttgaa gttggtcaag tgatctcagt tcgtgagaag tcaatcaaag ttccagctat
    13441 ccttgaagct gttgaagcaa ctcttggccg tccagctttt gtatcattcg atactgaaaa
    13501 attggaagga acactcactc gtttgccaga acgtgatgaa atcaacccag aaatcaacga
    13561 agcacttgtt gttgaattct acaacaaaat gttgtaatat tttattgaat atgagaggca
    13621 taaaagcctt gatacaaagc actttggagc tttattccaa ggtgcttttt aaatttttac
    13681 tacccttttt atgaaggaag agaatggtat tcatattttt ttattgccga aatgagatgg
    13741 tttcagttaa tggacctaag tccatagtct tccaacgata ttgcaaacgc tttctaaaaa
    13801 tggtaattta atccatagat acgtatcaat aaataataaa aaggaggagt tatggatact
    13861 cggattaacg aagcaatgca aaaattttcc agatcagtca ttgttcctgt caagttcatg
    13921 gctgtcatgg ggctattttt agcgataggg gttattctac aacttcagtt tatgcctgag
    13981 tttattcaaa cattgggctc attaatcaaa acgatgatgg acagcatgtt aaataatctg
    14041 tcattgattt tttgtatagg gattgcgtcc tctttggcaa ataaaaagaa agtagaggct
    14101 tctttaatag ccctcattgc ttttctattt ttcttggcag ctaacaatgc ctggttgaca
    14161 ctaacaaatc agttggcaag cgcaggtgag gttggactat ttgggactgg acaagggatg
    14221 gtattgggat tccaagtggt tgatatgaat gtctttttag gcatgatgat aggctgtctt
    14281 gttggttggt tccacaataa attttcagaa aaagaattta ttgatctatt tagtatttat
    14341 ggtggttctc gttttacatt cacgctcctc attcctgtca tcctagtttt agccattgcc
    14401 atgtgttata tttggccggt cgttaatcag ggaatttctt cattatctgg ttttatttta
    14461 actactggtt tggtaggagt atttatttac gcatttggta accgtttctt gattcctact
    14521 ggtctccacc acttactatg gatgccgttc tgttttacgg cgcttggagg aactgctgaa
    14581 atcaatggta aaatttacag cggagccgtt aatatctttt atgcagaact agcaaactca
    14641 ggttcaattg tctcgctaga taactcgttg aggtttgcga cctttggctt tgtcaaaata
    14701 tttggatcaa ttgctgttgc agcagcaatc atctattgtg ctagaaaaga aaagaggaaa
    14761 gaagttgaag ggatgatgtt gccgtcaaca tttgttgcaa tggcagcagg tattacggaa
    14821 ccgttggact ttaccttctt attcctatca cctctattat ggtttataca tagtttgcta
    14881 acagctattt cagaaacaat tttatgggct ttaggtgcta gaacttatat gctctatggg
    14941 ttgattgaca cagtcgtatc gaatgcacca tttagtccca atatcactaa gttttaccta
    15001 gtgattattg tggggattat catgacaatt gtctggtttg tcacttttgt ctttttaatt
    15061 aaaaaactgg atgttaaaac tcctggtaga gaagatatag tagaacctgt gtcagagaca
    15121 gtagcatcta tagtagaaac aatggatgat acggccctag tgattaaagg tttaggtggt
    15181 gcagataata ttgaacatgt cacgaattgt tttacacgtc ttcgagtgac tgtaaaagat
    15241 gagatgaaag tggatgacca tatattgcag aaggtttctc aacaaaaagg tgttgtgaaa
    15301 aatggcaaaa atattcaggt cattattggt atgggagttc agacatttaa agaagaagtc
    15361 tgtgctaccc taggaataac agagtaagaa aggatattat aatggtaaga gaaaagcaat
    15421 cagtcgttat tgcaggtgga ggaagtacgt acacagcggg aattgtgatg atgttaatca
    15481 ataaccaaga tagatttccc ctaagaaaat tgaaattcta tgataatgat aaagaacgtc
    15541 aagatattgt cgcaaaagcc tgcgaagtga ttgtacggga gcgtgctcca gaaatagaat
    15601 ttgttgctac gacagaccca gaagaagctt tttcagatgt tgattttgtc atggcacata
    15661 tccgagtggg cggattgaaa atgcgtgaac aggatgaaaa gattccgctt aaatatggag
    15721 tcgtcggtca agaaacatgt ggtccaggag ggattgccta tggcatgcgg tctataaagg
    15781 gagttattga attgattgat tacatggaaa aatacgctcc gaatgcatgg atgcttaact
    15841 attcaaatcc tgcagcaata gtggcagaag ccactcgtcg gatgcgacca gaatccaaaa
    15901 ttatcaatat ttgtgacatg cctgttgcca ttaaaaaatc aatggcagat attttgggct
    15961 tgaagaatga aaatgagatc attgatcgtt actatgggtt gaaccatttt ggatggtgga
    16021 cggaaatgta tgataaggaa ggcaatgact taatgccaat cttacgaaaa catgtgcaaa
    16081 aatatggtta tgcagcagct acagatgaac ataatccttt actggaagag gctagttgga
    16141 aagacacctt cttaaaagtg aaagatgtcc aagcagttga tcaggcaact ctgccaaata
    16201 cttatttgaa atattatctt taccctgact atgttgttgc gcactctaac ccagattata
    16261 ctcgtgcaaa tgaagtcatg gatcatcgtg aaaaaaatgt ctttgcagaa tgcaaacgaa
    16321 ttgccgatca tcagacggca gctgatacca gtattgaagc aggggaacat gcagaattca
    16381 ttgtccaact ggctggtgct ttggcctata atacctatga gagaatgctt cttattgtca
    16441 aaaacgaagg agcaatcgct aatatcagtg aggatgcaat ggttgaagtc ccttgtattg
    16501 taagcaagcg tggctatgag ccattgtgca tgggtagcat tccgcatttc caaaaaggaa
    16561 tgattgaaca gcaagttgct gttgagaaat tggtagtaga cgcctatcaa caagagtctt
    16621 atgaaaaact atggcaagct cttagtctat ctaaaacagt gccatcagcc accgtagcaa
    16681 aacaaatttt ggatgagctg attgaagcaa ataaagaata ctggcctact ttagcatagt
    16741 ggttatttat ataaaaatat ggtataaata atcagtaaca aaaaatatgg gaagataggg
    16801 tggacttcta tatgcgtttc acagatatag tcaatgaaca ttacagtgaa cttaattcat
    16861 cagaattatt gatgtgtaat tacattatag aaaatgctgc gaccataccc aaaatgggag
    16921 tggtggaatt tgcaaggaga agtctgtcct caaagtcttc cgttattcgt ttttcacaga
    16981 aattaggctt tacgggtttt acagaattaa aaaattttat aaagtggcag gatcaggaag
    17041 aggtactgat tgagcaacta acctttaagg agcaggtgac aaaggatatt cattctttgc
    17101 tagagttgat agcaaaaagc gattggtcgc ctatctatca aagactggat acaattgata
    17161 atatttatgt gattacaaca ggggtagctc agaagaatca agcagcagaa ttgcaacgct
    17221 tatttctctt aatcggaaag cccctgcaga ttgtgccagg agatgggaat tcaaatgagt
    17281 tccgaagaat tatagaacgc ctaacggcta aggacatcgt atttgtatta tccttatctg
    17341 gtgaaaataa taatttggaa tcaatcgtta agcgtttaaa attggtaaaa agtaatatcg
    17401 tttcgattac aagcttacag aataattggt tatcaggaaa atcagatttt aatctttatg
    17461 cgacaaccag tcggagtccc ctccctaggg attggtggct ccaaacaact tcctccttct
    17521 ttgtattgat agaagccttt acctttgggt atgtggatta taagaagcga atcaagtaat
    17581 ttgggtacgt caaacgcatt aagaaaaata gttttcagaa aac
//
LOCUS       DATUCG010000041        17420 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.042, whole genome shotgun sequence.
ACCESSION   DATUCG010000041 DATUCG010000000
VERSION     DATUCG010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17420
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.042"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(29..868)
                     /locus_tag="VCA20_001073"
     CDS             complement(29..868)
                     /locus_tag="VCA20_001073"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEP1834912.1"
                     /translation="MTKADIIFKENIRKIMEEGVFSENARPRYKDGNVANSKYVTGTF
                     AEYDLSKGEFPITTLRPIPIKSAIKEILWIYQDQTNDLSVLQEKYGVQYWNDWEVEGT
                     GTIGERYGAIVKKHDIISKILKQLEENPWNRRNVISLWDYEAFDQSPGLLPCAFQTMF
                     DVRRVDGNIYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNL
                     HIYDNQFEQAEELLRRTPSEVEPRLLLNVPDGTNFFDIRAEDFELVDYNPVKPQLKFD
                     LAI"
     gene            complement(921..1223)
                     /locus_tag="VCA20_001074"
     CDS             complement(921..1223)
                     /locus_tag="VCA20_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834913.1"
                     /translation="MNLTLLKRLNLVLYGIAIFLIVMLFLPIGQWFDIVNVNFKLTFF
                     IIPFFGLASLPTAIYTKNVRQILLSVLLIALYFILFSLITALSGLFHLNFYSFFFK"
     gene            complement(1235..1513)
                     /locus_tag="VCA20_001075"
     CDS             complement(1235..1513)
                     /locus_tag="VCA20_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834914.1"
                     /translation="MKQVNMSKIINYLTILGLLILLSAFFLDNWIRDWFFPSSWGNVA
                     TMLILPLLGALILILSIYYKKLWTGLISIFLMISFPLIFGIGYFIFGP"
     gene            complement(1790..2749)
                     /locus_tag="VCA20_001076"
     CDS             complement(1790..2749)
                     /locus_tag="VCA20_001076"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEP1834915.1"
                     /translation="MSKKIIGIDLGGTSVKLAILTTEGEIQEKWSIKTNILDDGSHIV
                     PDIIDSIKQRFETHGLTKDDFLGVGMGSPGVVDSEAGTVIGAYNLNWKTLQLVKDQFE
                     AALGLLFFIDNDANVAALGEQWVGAGNNNPNVVFMTLGTGVGGGVIAAGNLIRGVKGA
                     GGELGHITVDFDKPFACTCGKKGCLETVASATGIVNLSRRYADQYAGDAKLKQMIDDG
                     QDVTAKDVFDLAKEGDDLALIVYRHFSEYLGVACANIAAVLNPAYIVLGGGVSAAGEF
                     LLDGVRKVFAENSFPQIKESTQIVLATRGNDAGVLGAASLVLK"
     gene            complement(2845..3180)
                     /locus_tag="VCA20_001077"
     CDS             complement(2845..3180)
                     /locus_tag="VCA20_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002934966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834916.1"
                     /translation="MKVLVMSYMVIYLLVTLGAALFSYLKTQKMNTLRLILTILSMIL
                     LTSTLYFYSQSYHDLQMVGFALGFTFISTLFLYNGTKEGSNFTTVMLFSIGRFILHIQ
                     FLILLYLFR"
     gene            complement(3181..5049)
                     /locus_tag="VCA20_001078"
     CDS             complement(3181..5049)
                     /locus_tag="VCA20_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEP1834917.1"
                     /translation="MSDFIVEHLTKSVGDKTIFADLSFIIHQGDRIGIIGVNGTGKTT
                     LLDVLSGRIGFDGDVSPFRTKNAYKIAYLTQEPDFDESKTVLDTVLSSDLRETQLIRE
                     YELLLSHYDEASQVRLEKVMAEMDSLNAWEIESQVKTVLSKLGLTDLNKTVAELSGGL
                     RRRVQLAQVLLGNADLLLLDEPTNHLDIDTIEWLTTFLKNTKKSVLFITHDRYFLDNV
                     ATRIFELDRASLTEYQGNYQDYVRLKAEQDERDAALRHKKEQLYKQELAWMRRQPQAR
                     ATKQQARINRFHDLKGDLTNKIDDSELEINFETSRIGKKVINFENVSFSYPDKPILAD
                     FNLLIQNKDRIGIVGDNGKGKSTLLNLISGDLQADSGKVDIGETIRIGYFSQTIKGLD
                     ESKRVINFLQEVAEEAKTTSGMVSIAELLEQFLFPRNTHGTLIEKLSGGEKKRLYLLK
                     ILLQRPNVLLLDEPTNDLDIATLTVLEHFLQGFTGPVITVSHDRYFLDKVANKILAFE
                     DSSIETFFGNYTDYLDEKAFQASSSAISLEKPKEKTEKIKENKKRMSYFEKQEWATIE
                     EDIADLEERIAAVEAEMLICGSDFTKLSDLQKELDEKNDLLLEKYERYEYLSELEG"
     gene            complement(5042..6253)
                     /locus_tag="VCA20_001079"
     CDS             complement(5042..6253)
                     /locus_tag="VCA20_001079"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEP1834918.1"
                     /translation="MKLNNLPSEFQEALPILEKIKAAGFEAYFVGGSVRDAILGRPIH
                     DVDIATSSYPQETKQIFSRTIDVGIEHGTVLALEGKKEYEITTFRTEEEYVDYRRPSQ
                     VSFVRSLEEDLKRRDFTVNAFALDEEAQIIDLFEGMTDLENCTLRAVGIPAERFNEDA
                     LRIMRGFRFAATLDFEIEPTTFTAMVETAPLLEKISVERSFIEFDKLLMADFWRKGLR
                     AMIDSKAYNFLPDLAGKSDELEAMLTSLTEEFCFSTSEQAWAMLFVCLGIDNIKSFLK
                     KWKTSNDFQRSVVKLVEIYQLRQAGPVTKQICFKYGKEFLYLVEELRQAQGLVTDFAA
                     IDNIDQALTIHDKHEIVVNGGHLMKAFDLKPGPVLGELLKEVEFQIVEGQLENEEQAI
                     MTFVKGILENE"
     gene            complement(6250..7017)
                     /locus_tag="VCA20_001080"
     CDS             complement(6250..7017)
                     /locus_tag="VCA20_001080"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEP1834919.1"
                     /translation="MTIKVIIAGFKGKMGSTAVEMVKGDAALSLAALVDPFATETEVD
                     GVPVFKTKEEVASLEADVWVDFTTPKFAYENTRFALENGFAPVVGTTGFTPEEIEELT
                     ALSAEKGLGGLIAPNFAIGAILLMQFAAQAAKYFPNLEIIELHHDKKKDVPSGTAVKT
                     AELISQVRQSQTQGAADEEELIAGARGAEFDGFRIHSVRLPGLVAHQEVIFGAQGEGL
                     TIRHDSYDRISFMGGVNLGIKEVVKRSQLVYGLEHLL"
     gene            complement(7026..7877)
                     /locus_tag="VCA20_001081"
     CDS             complement(7026..7877)
                     /locus_tag="VCA20_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEP1834920.1"
                     /translation="MKLAVITDSSAVLNVQSIERDDLFVLSIPVSIDGENYIEGQNLT
                     VEEFYQKMASSKELPKTSQPSLMELEEILAKLTEKGYTHALGLFLSSGISGFYQNIQY
                     LADAFEGLTVAFPDTKITSAPLGMMVENVLKWADAGLSFEEITGKLDKEIGKTTAFIM
                     VDDLNHLVKGGRLSNGAALLGNLLSIKPILYFTGEGKIEVYEKVRTEKKAIKRLLEIL
                     QEQTTEGQYQIAIIHANAPQKAENFKQQLEEAGVGSDLPIVSFGSVIGTHLGEGAVAF
                     GISPIIE"
     gene            complement(7879..8253)
                     /locus_tag="VCA20_001082"
     CDS             complement(7879..8253)
                     /locus_tag="VCA20_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEP1834921.1"
                     /translation="MEIIRDKEFVNQYHFDARNHAWEKENGIPETKLKVDFQLIEQNR
                     AENRTSMITILRFMIVLDHFVISGAMSQAVHLPNRLVEEPTEFTDEEKRVLVEPLLDI
                     LKRMTYEVTEIAFDAPGVNLEF"
     gene            complement(8349..10304)
                     /locus_tag="VCA20_001083"
     CDS             complement(8349..10304)
                     /locus_tag="VCA20_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEP1834922.1"
                     /translation="MKIQDVLRKDVMLLDLQATSKEAVIDEMIASLVDKGYVSDFDVF
                     KTGIMNREAQTTTGLGDGIAMPHAKNAAVKEATVLFAKSNKGVDYASLDGQPTDLFFM
                     IAAPEGANDTHLAALAELSKYLMKAGFADKLRSVSSPEEVIAVFDEAEAADKAAEEVV
                     AAPSGDRPFIVAVTACTTGIAHTYMAEEALKKQASEMGVDIKVETNGASGVGNKLTAE
                     DIENAAGVIIAADKAVEMPRFNGKPLVSRPVAAGIKQPEELINLILDGKASVYTATEG
                     AVAVESSEKLSLGKAFYKHLMSGVSQMLPFVIGGGILIALAFLFDGALGVPQDSLGSL
                     GSYHEIAAMFMKIGGAAFGFMLPLLAGYIAYSIAEKPGLVAGFVAGAIANSGLAFGKI
                     PYAAGGEETLALAGVSSGFLGALVGGFLAGGVVLVLRNALRNLPKSLQGLNAILLLPL
                     LGTAITGFLMFFVNIPMAAINTGMNNFLAGLEGSSAILLGLVLGGMMAVDMGGPVNKA
                     AYVFGTGTLAATVADGGSVAMAAVMAGGMVPPLAVFVATLLFKDKFTQEERNSGLTNI
                     VMGLSFITEGAIPFGAADPARAIPSFIAGSALAGALVGLSGIQLMAPHGGIFVIALTS
                     NPLLYLAYVAIGAVVSGVLYGVLRKAK"
     gene            complement(10301..11212)
                     /gene="pfkB"
                     /locus_tag="VCA20_001084"
     CDS             complement(10301..11212)
                     /gene="pfkB"
                     /locus_tag="VCA20_001084"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019323947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEP1834923.1"
                     /translation="MIYTVTLNPAIDYIVRLDRVETGSVNRMESDDKYAGGKGINVSR
                     VLKRLGYPNTATGFLGGFTGQFIKDGLIAEGIDTDFVQVDQDTRINVKIKADQETEIN
                     GLGPIVTDTQLAELEMVLAGLTKEDTVVFAGSAPASLGNEVYNRLIPVAKKAGAQVVC
                     DFEGQTLLDSLAHQPLLVKPNNHELEAIFNVELNGIADIETYAKKILEMGAQNVIISM
                     AGDGALLVTPAATYFAKPIKGTVKNSVGAGDSMVAGFTGEYVRSQDPIEALKWGVACG
                     TATAFSDDLASIEFIKETYEKVEVETL"
     gene            complement(11209..11958)
                     /locus_tag="VCA20_001085"
     CDS             complement(11209..11958)
                     /locus_tag="VCA20_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEP1834924.1"
                     /translation="MNILKSERKQIILERIHSQQFVRLEELIEVLDTSESTVRRDLDE
                     LENDGKLRRVHGGAEAPGNLQLEESILEKSVKNVQEKREIAERAARQIVNGDVIFLDA
                     GTTTGVLIDYLQQENLTVVTNSIHHAVRLVERKIKTIIIGGFVKQSTDASVGAVALEQ
                     IRQLNFDKAFLGMNGIDKNYFTTPDIEEATIKRTIIDNAKESYVLADASKIGQFSFAK
                     VAAIEKANIICQSSESSLLDIIKEKTRVIEV"
     gene            complement(12054..13109)
                     /locus_tag="VCA20_001086"
     CDS             complement(12054..13109)
                     /locus_tag="VCA20_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEP1834925.1"
                     /translation="MKRKVFEHYQPYKIKQKARFDLGNNENRIIDWSFLPQETLENLD
                     VNQLSFYGDNTYSELIEKYAAYLGVNPKQVTVGVGSDHLIHMIVSTFMEKDDTFLTVN
                     PDFFMYETYNHMHGSRFETIDLEAKNDTLVLPVEKLLARAEEGKVKIIMLSNPNNPSS
                     VAYSLEALEKLAISFKGLLVVDEAYIEFANVESFISRLQQFDNVLVLRTLSKAFGLAG
                     LRVGFAVGSEELIYELDKVIPPFSLSNLTAKMAAVALNYTDKVLETVGVIQNLREEMI
                     LFLQGLDNCQVLPSQASFVTFKAPWAEEFYQQALAQDWNFKYYPTGKLEGHIRLSIGR
                     PEEMDMMKKMIEKLVEA"
     gene            complement(13140..13865)
                     /gene="trmD"
                     /locus_tag="VCA20_001087"
     CDS             complement(13140..13865)
                     /gene="trmD"
                     /locus_tag="VCA20_001087"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEP1834926.1"
                     /translation="MRIDILTLFPEMFAPLEHSIVGKARDKGLLEINYHNFRENAEKA
                     RHVDDEPYGGGQGMLLRAQPIFDTMDSIEQTKPRVILLDPAGRTFNQAYAEELAQEEQ
                     LIFICGHYEGYDERIKTLVTDEISLGDYVLTGGELAAMTMIDATVRLIPEIIGKEVSH
                     TDDSFSSGLLEYPQYTRPYEYRGMVVPDVLMSGHHENIRKWRLEESLRKTYQRRPDLL
                     ENYNFTAEELTIFEKIKAEDTVD"
     gene            complement(13855..14373)
                     /gene="rimM"
                     /locus_tag="VCA20_001088"
     CDS             complement(13855..14373)
                     /gene="rimM"
                     /locus_tag="VCA20_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEP1834927.1"
                     /translation="MNYFNVGKIVNTQGLQGEMRVLSVTDFAEERFKKGAKLALFNDK
                     DQFVMEVEIASHRKAKNFDIIKFKGMYHINDIEKYKGFSLKIAEENLSDLEDGEFYYH
                     EIIGLDVYENDQLIGQIKEILQPGANDVWVVKRKGKKDLLLPYIPPVVLNIDIPNNRV
                     DVELLEGLDDEN"
     gene            complement(14466..14846)
                     /locus_tag="VCA20_001089"
     CDS             complement(14466..14846)
                     /locus_tag="VCA20_001089"
                     /inference="COORDINATES: protein motif:HMM:NF028534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5301 domain-containing protein"
                     /protein_id="HEP1834928.1"
                     /translation="MKKRWLIGVVLSCISLLFGYQFLKKTEIRFPQANQIVISNQDGG
                     EIRTLKGSEMSDFLSELTQIHPYLIKNASNHDQPVGVEEYYQLTFQPENKMAYLYEKN
                     GKTYLEFPYELTVRTKKPLSELID"
     gene            complement(14915..16252)
                     /locus_tag="VCA20_001090"
     CDS             complement(14915..16252)
                     /locus_tag="VCA20_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEP1834929.1"
                     /translation="MYQTRNNKEKLWLFIKIFLPILIYQFANYSASFIDTMMTGQYST
                     MDLAGVSMATSLWNPLFSFLTGIVSALVPIIAQYLGKGERDKIREEFHQFVYLAIGLT
                     ILLLCSILFIAVPALAQFGLDESVFQVGRQYLYFISIGILPLLLFSVCRSFFDALGLT
                     QLSMYLMLLLVPFNSLFNYLLIYGKMGLPALGGAGAGLGTALAYWAVLLVIVLVMCKN
                     KTISSYQIWQWSPIDGALLKEGLKIGLPIGLQVFAEVAIFAVVGLYMAKFSAQIIAAH
                     QAAMNFATLLYAFPSSVSSALAIVVAYEVGANRPLDVKNYSCLGRLVALGFAGLTLTF
                     LYFFRSKVAYLYGNDTDFVRLTSHFLSFALMFQLADAYTAPIQGILRGYKDTTVPFVL
                     GLVAYWSMTFPVAFFLERFFHLGPEAYWIGLISGIFVCGIALRLRLKKIASIQ"
     gene            complement(16322..17305)
                     /gene="guaC"
                     /locus_tag="VCA20_001091"
     CDS             complement(16322..17305)
                     /gene="guaC"
                     /locus_tag="VCA20_001091"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017769630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEP1834930.1"
                     /translation="MFNETPVFDYEDIQLIPNKCIINSRSEADTTVTLGKYSFKLPVV
                     PANMQTIIDEDVAEMLAKEGYFYIMHRFDEAGRIPFIKRMHEQGLIASISVGVKEYEY
                     EFVTSLKADAPEFITIDIAHGHAESVIKMIQHIKKELPETFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIA
                     KSIRFGASMVMIGSLFAGHIESPGKTIEVDGEKFKEYYGSASEYQKGAYKNVEGKKIL
                     LPAKGHLKDTLVEMEQDLQSSISYAGGRDITSLKHVDYVIVKNSIWNGDSI"
BASE COUNT         5146 a         3983 c         3317 g         4974 t
ORIGIN      
        1 agaacgaagt tcttcactgc taatccagtt aaattgctaa atcgaatttt aactgcggtt
       61 taactggatt gtagtccacc aattcaaaat cttctgcccg aatatcaaag aaattggtac
      121 cgtcaggaac attgaggagc aaacgtggct caacttcaga tggtgtgcga cggagtaatt
      181 cttcagcttg ttcaaactga ttgtcataga tgtgaagatt gttgatgaaa tagaaaaact
      241 tcccaacctt ccagccaaaa tgtttagcaa tcatcatctg aagtgctaca tactgcatag
      301 cgttgatatg atgggctact agcatatcat ttgagcgctg agtgagggtc gcatcgagat
      361 aaatgtttcc gtccactcgg cgaacatcaa acatagtttg aaaggcacat ggcaataggc
      421 ctggcgattg gtcaaaagcc tcataatccc acaaggaaat cacattgcgg cgattccagg
      481 gattttcctc caattgcttg aggatttttg aaatgatgtc atgcttttta acaatagcac
      541 cgtaacgttc accgattgtg cctgttcctt caacttccca atcattccaa tactggacac
      601 cgtatttttc ctgcaagaca gataggtcat tggtctggtc ttggtaaatc cagagaattt
      661 ccttgatggc cgatttaatc ggaattggtc taagtgttgt aataggaaac tcgcccttag
      721 acaagtcata ctcagcaaag gtgcctgtaa cgtacttgga attagcgaca tttccatcct
      781 tgtaacgtgg gcgagcattt tctgaaaaaa cgccctcttc cataatctta cgaatgtttt
      841 ctttaaaaat aatatctgct ttagtcatgg tcttagtata gcaaattcta gcaaaaaact
      901 ccagttttgc accggagttc tcatttgaaa aagaatgaat aaaaattaag gtggaacaaa
      961 ccagataggg cagttattag gctgaacaat ataaaataca aggcaatcaa gagaacactc
     1021 aacaatatct gacgaacatt tttagtgtaa atagccgttg gtaagctggc tagtccgaaa
     1081 aatggaatga tgaaaaatgt tagtttaaaa ttcacattta caatatcaaa ccattgccct
     1141 attggcaaaa atagcatgac gatgagaaaa attgcgatac catacaaaac tagatttaat
     1201 ctttttagta aagtcaggtt cataaccgct ccttctaggg gccaaatata aagtagccaa
     1261 taccgaatat cagaggaaat gagatcatta agaagatact aatcaaacca gtccacaatt
     1321 ttttataata gatagagagt ataagaatta gagcccccag tagagggaga atcaacatgg
     1381 ttgctacgtt tccccagcta gacgggaaaa accagtcacg aatccagtta tcaaggaaaa
     1441 aagcagataa caaaatcaat aaaccaagaa tggtcagata gttgattatt ttagacatgt
     1501 ttacctgttt cataaggtga cactcctttc tctttacact ttcattgtac gcttaaacac
     1561 tacatttttc aatatatgta tataaagaaa atgtgacagc ggaattgaaa aaagggagtg
     1621 ggaaagaact cgacaattca tagaagagtt cgtcttccca cccccgcaca gttgattagg
     1681 tcagatttgg agggtgaaac acgaataaat ctgccaatca accactgcgc tgagatgttg
     1741 acactaacgt tgagaagtgg tgctgggctt gagcccagcc tctcagttat tatttcaaaa
     1801 caagtgatgc tgcgcccaat acacctgcat cattaccacg tgttgccaag acaatttgtg
     1861 tactttcctt gatttgtggg aagctatttt cagcaaagac cttgcgaacg ccatccaata
     1921 agaactctcc tgcagctgat acaccgccac ctagaacgat gtaggctgga ttgaggactg
     1981 cagcaatgtt tgcacaagca acacctaggt attctgagaa atgacggtag acaatcaaag
     2041 ccaaatcatc gccttctttt gccaaatcaa agacatcttt agccgttaca tcttgcccat
     2101 catcaatcat ttgtttcagt ttagcatcgc cagcgtattg atcagcatag cgacggctaa
     2161 ggttgacaat acctgtcgct gaagcaactg tttctagaca acctttctta ccacaagtac
     2221 atgcaaatgg cttatcaaag tcaactgtga tatggcccaa ttcaccacca gcgcctttga
     2281 ccccacgaat caagtttcca gcagcgatga caccgccacc gacacctgta ccaagcgtca
     2341 taaagacaac gtttggattg ttgtttccag caccaaccca ctgctcacca agagctgcta
     2401 cgttggcatc attgtcgata aagaatagta aaccgagcgc agcttcaaac tgatctttaa
     2461 ccaattgcaa agttttccag ttgaggttat aggcaccgat aactgtccca gcctcactgt
     2521 caacaacgcc tggtgaaccc atacctacac ccaaaaagtc atcttttgtc aaaccatggg
     2581 tttcaaatcg ctgcttgata ctgtcaataa tatcaggaac aatatgactc ccatcatcta
     2641 aaatatttgt cttgatagac catttttctt gaatttctcc ctcagtagtc aaaatggcca
     2701 atttaacaga agttccccca aggtcaatac cgataatttt tttagacata atcatcacct
     2761 caaatgtatt ttctttatta taacacaatg caaaggtttg cgtaaagcct tttcaaaaat
     2821 ttgtacggat aaaaataaat tgctctatcg aaagagatag agcaatatca aaaattgaat
     2881 atgtaaaata aagcgaccga tagaaaagag cataactgtc gtaaaattac taccttcttt
     2941 cgttccatta tagagaaaaa gagtagaaat aaaggtaaaa cctagagcaa accccaccat
     3001 ctgcaagtcg tgataagact gactataaaa atacaaggtg cttgtcagca atatcatgga
     3061 taggatagtt aaaatcaagc gcaatgtatt cattttttgg gtctttagat agctaaacaa
     3121 agccgctccc agggtcacca agagatagat gaccatatat gacatgacaa gaactttcat
     3181 ctagccctcc agttcgctca gatactcata ccgctcatat ttttccaaga gcagatcgtt
     3241 tttctcatcc aattccttct gcaaatcgga caattttgta aaatcactac cacatatcaa
     3301 catctcggcc tcaacagccg caatccgctc ctccaagtca gcaatatcct cttcgatggt
     3361 tgcccattcc tgcttctcaa agtaggacat ccgcttcttg ttctctttga ttttctcggt
     3421 tttctccttt ggtttttcca aggaaatggc agagctggaa gcctgaaagg ccttctcatc
     3481 cagatagtct gtgtagttgc caaagaaggt ctctatactg ctatcttcga aagccaagat
     3541 tttattggct accttgtcta ggaaataacg atcgtgacta acggtaatga ctggaccagt
     3601 aaagccctgc aagaaatgtt ccaagactgt caaagtcgct atgtccagat cgttggtcgg
     3661 ctcgtccagt aagagaacat tgggccgttg taaaagaatt ttcagcaagt aaagccgctt
     3721 cttctcccca cctgacaatt tctcaatcag ggtaccatgg gtattgcgtg ggaagaggaa
     3781 ttgctccaag agttctgcga tagaaaccat accagatgtc gtcttagctt cttctgccac
     3841 ttcctgcaag aaattgatga cccgcttgct ttcgtccaag cccttgatgg tctgagaaaa
     3901 ataaccgata cggatggttt caccaatatc taccttaccg ctgtctgcct gcaaatcacc
     3961 tgagattaga ttgagtaagg tagattttcc tttaccattg tcaccaacaa tacctatgcg
     4021 gtccttgttt tgaatgagca ggttaaagtc tgctaaaatg ggcttgtcag gataggaaaa
     4081 gctgacattt tcaaagttaa tgactttttt gccgatacga gaggtttcaa aattgatttc
     4141 cagttcgcta tcgtctatct tgttggttaa atcgcctttg aggtcatgga aacgattgat
     4201 acgagcctgt tgcttggtag cacgagcttg aggttgtctg cgcatccaag ccaactcctg
     4261 cttgtagagc tgctctttct tatggcgaag ggcggcatct cgctcgtcct gttctgcctt
     4321 caagcgaaca tagtcctggt aatttccctg atattcggtt aagctggcgc gatccaattc
     4381 aaaaatccgt gtcgccacat tgtccaagaa atagcgatcg tgggtaataa agagaacaga
     4441 ctttttagta tttttcaaaa aagtcgtcaa ccattcaatg gtatcaatgt ccaggtggtt
     4501 agtcggttca tctagcaaaa gcaagtcagc attgccaagg aggacttggg ccaattggac
     4561 tcgtctacgt agaccacctg acaactcagc aacggtttta ttgaggtctg ttagaccgag
     4621 tttcgacaag acagtcttga cctggctttc aatctcccaa gcattgagag aatccatctc
     4681 agccatgact ttctccagtc ttacttgact ggcttcatcg taatgagata agagcagctc
     4741 gtattcacga atcagctggg tttcacggag gtcagatgaa agaacggtgt ctaaaactgt
     4801 cttactttca tcaaaatcag gttcctgggt cagataggca attttatagg cattttttgt
     4861 acgaaaaggt gatacatctc cgtcaaaacc gatacgacct gacagaacat ccaacaaagt
     4921 cgtctttcct gtaccattga ccccaataat cccgatacgg tcgccttgat ggatgatgaa
     4981 agacagatca gcaaagatcg ttttatctcc gacagattta gtcagatgtt caacgataaa
     5041 atcactcatt ctctaagatt cctttcacaa atgtcataat cgcttgctct tcgttttcca
     5101 gctgtccttc cacaatctgg aattccacct ctttgagtag ttctcccaat actggacctg
     5161 gtttgaggtc aaatgccttc atcaaatgtc ctccattgac aacaatttca tgcttgtcgt
     5221 ggatagtcaa ggcttggtca atattatcga ttgctgcaaa atctgtgaca agaccttgtg
     5281 cctgacgaag ttcctctacc aagtacaaga attctttgcc atacttgaaa cagatttgct
     5341 tggtaacagg tcctgcttgg cggagttgat aaatctctac caacttgacc acgctacgtt
     5401 ggaaatcatt gctggttttc cattttttca ggaaggattt gatgttatcg attcctaggc
     5461 agacaaagag catggcccaa gcttgttcag atgtcgaaaa gcaaaattcc tctgtcagac
     5521 ttgttagcat ggcctctaat tcatcacttt ttccagctaa atcaggcaag aaattgtaag
     5581 ccttggaatc aatcatagcg cgcaagcctt ttcgccaaaa atccgccatc aagagtttat
     5641 caaactcaat aaagctacgt tccaccgata ttttttccaa aagcggtgcg gtttcaacca
     5701 tagctgtgaa ggtagtcggc tcaatctcaa agtccaaggt cgccgcaaag cgaaaacctc
     5761 gcatgatacg gagagcatct tcgttgaaac gctcagccgg gatgcctaca gcccgtaggg
     5821 tgcagttttc taagtcagtc atcccttcaa aaaggtcaat gatttgagcc tcttcatcca
     5881 gagcaaaggc gttgactgtg aagtcgcgac gtttgagatc ctcttctaag gaacgcacaa
     5941 aagaaacctg acttggacga cggtagtcta catactcttc ctctgtccga aaggtggtaa
     6001 tttcatactc cttcttcccc tctaatgcta acaccgtacc gtgttcaatt ccgacatcaa
     6061 tagtccgtga aaagatttgt ttcgtttcct gagggtagct ggatgtcgca atatcgacat
     6121 catgaatagg cctaccgaga atggcatcac gaacggaacc gccaacgaaa taagcctcaa
     6181 agccagctgc tttaattttc tctaaaatcg gcaaagcctc ctgaaactca gaaggcagat
     6241 tgtttaattt cataataagt gttccaaacc atatacgagt tgtgagcgtt tgactacctc
     6301 tttaatgcca aggttgacac cgcccataaa ggaaatacga tcgtaagagt catgacgaat
     6361 ggtcaagcct tctccctgtg ccccaaaaat cacttcctga tgggcaacaa ggcctggcaa
     6421 gcgtactgag tggatacgga agccgtcaaa ttctgcaccg cgagcgccag caatcagttc
     6481 ttcttcatcc gctgcacctt gagtctgtga ctgacggact tgtgaaatga gttcggccgt
     6541 tttgacagcc gtaccactcg gtacatcctt cttcttatcg tggtgtaatt caatgatttc
     6601 aagatttggg aaatacttgg ctgcctgtgc tgcaaattgc atgagcaaga tagccccgat
     6661 tgcaaagtta ggagcaatca agccgcccaa gcccttctca gctgacaagg ctgtcaattc
     6721 ctcgatttct tcaggggtaa atcctgtcgt tccaaccaca ggtgcgaaac cattttccaa
     6781 ggcaaaacgg gtgttttcat aggcaaattt tggcgttgta aaatccaccc agacatcagc
     6841 ttctaggctg gcaacttctt ctttggtctt gaaaacagga acaccatcta cttctgtttc
     6901 tgtcgcaaag ggatccacca aggcagccag actaagcgct gcgtcacctt tgaccatttc
     6961 aacagccgtt gagcccattt ttcctttgaa tcctgcgata attaccttaa ttgtcatgca
     7021 aactcctatt caataatggg agaaatacca aaggccacag ctccttctcc caagtgcgta
     7081 ccgatgaccg aaccaaatga cacgattggt aaatcgctac cgacacctgc ttcttccaac
     7141 tgctgcttaa agttttcagc cttctgaggg gcgttggcgt gtatgatagc aatctgatac
     7201 tgtccttcgg tcgtttgctc ttggaggatt tccaagaggc gtttaatggc ctttttctcc
     7261 gtccgaactt tttcatacac ttcaatctta ccctcacctg taaaatacag aatcggtttg
     7321 atgctcaaaa gattgccaag caaggctgca ccattggaca agcgaccacc ttttaccaaa
     7381 tgattgaggt catcaaccat gatgaaggct gtagtcttcc caatttcttt gtccaatttc
     7441 ccagtaatct cttcaaaact aagtcctgca tcagcccatt tgagaacatt ttctaccatc
     7501 atgcctaaag gagccgatgt gattttggta tctggaaagg caacggttaa tccttcaaat
     7561 gcatcagcca aatattgaat attttgataa aaacccgaaa tacctgatga caagaataag
     7621 ccaagtgcgt gtgtatagcc tttctctgtt aatttagcca gaatttcctc tagttccatc
     7681 aaactcggtt gacttgtttt aggtaattct ttcgatgaag ccattttttg ataaaactct
     7741 tcaaccgtca aattttgccc ttcgatataa ttttccccat cgatgctgac tggaatactt
     7801 aggacaaaca ggtcatcccg ctcaatactt tgaacattca aaacagccga tgagtcagtt
     7861 ataactgcta atttcatatt aaaactccaa attcactcct ggtgcatcaa aggcgatttc
     7921 agtaacctca taagtcattc gttttaaaat gtccaaaaga ggctctacca aaacacgttt
     7981 ttcctcatca gtaaattcag ttggctcttc taccaatcga tttggtaagt gaacggcctg
     8041 actcatggca cctgaaatca caaaatgatc tagtacaatc ataaagcgca aaatggtaat
     8101 catagatgtt cgattttctg cacgattttg ctcaatcaat tgaaagtcaa ctttcaattt
     8161 tgtttccgga attccgttct ctttttccca cgcgtgattg cgggcatcaa aatgatactg
     8221 attgacaaat tctttatcgc gaatgatttc catttcaaga ctcctctaat aacaataata
     8281 ctcacattat accataatcc tcgtgaaata gctaaaaaac cttgccagaa gcaaggtttc
     8341 aattaaaatt atttagcttt acgaagtacg ccgtaaagaa cacctgatac tactgcacca
     8401 attgctacat aagcaaggta aagaagtggg ttagaagtca aagcaataac gaagatacct
     8461 ccgtgtggcg ccatcaattg gatgcccgac aaaccaacca aggcacctgc aagggcagaa
     8521 ccagcgataa agcttgggat tgcacgagct gggtcagcag caccaaacgg aatcgcgcct
     8581 tcagtgatga atgaaagacc cataacgatg tttgtcaaac ctgagttacg ctcttcttgt
     8641 gtgaatttgt ctttgaaaag aagggttgca acgaatactg ccaacggtgg aaccatacca
     8701 ccggccatta ccgctgccat ggcaacagaa ccaccatctg caaccgtcgc tgcaagtgta
     8761 cctgtaccga agacataagc tgccttgttg actggaccac ccatatcgac tgccatcata
     8821 ccaccgagga cgagacctag gagaatagca gagctacctt caagaccagc aaggaagttg
     8881 ttcatgcctg tgttgatagc tgccattggg atgttgacaa agaacatcaa gaaaccagtg
     8941 atagctgtac ccaagagtgg caaaagcaag atggcattca aaccttggag tgattttggt
     9001 aggttacgca aagcgtttcg aagaacaagg accacaccac cagcaaggaa accaccgaca
     9061 agggcaccca agaaaccaga tgatacacca gcaagggcaa gggtttcttc accaccagct
     9121 gcgtaaggga ttttgccaaa tgcaagacca ctgttggcga tggcgccagc tacaaaacct
     9181 gcaaccaaac ctggtttttc agcgatagag taagcaatgt aacctgctag aagtggcaac
     9241 atgaagccga aggctgctcc accgatcttc atgaacatag ctgcgatttc atggtatgaa
     9301 ccaagactac caaggctgtc ttgtggcaca cctaaagctc catcgaatag gaaggcaagg
     9361 gcaatcaaga taccgccacc gataacgaat ggcaacattt gagaaacacc actcatcaag
     9421 tgtttgtaga aggctttacc aagactgagt ttttctgatg attccacagc gactgctcct
     9481 tctgttgctg tatagaccga tgctttccca tccaagatta gattgattaa ttcttctggt
     9541 tgtttgatac ctgctgcaac tggacgagat accaacggct taccattgaa acgtggcatt
     9601 tcaacagcct tatctgctgc gatgataaca cctgctgcat tctcgatatc ttcagcggtt
     9661 agtttatttc caacacctga agcaccgttt gtttcaacct tgatgtcaac acccatttca
     9721 gacgcctgct tctttagtgc ttcttccgcc atgtaagtat gagcaatacc agttgtacaa
     9781 gctgtaactg caacgataaa tggacggtcg ccacttggtg ctgcaactac ttcttcagca
     9841 gccttatctg ctgcttcggc ttcgtcaaag actgcaatga cttcttctgg actagataca
     9901 ctacgaagtt tgtcagcaaa accagctttc atcaagtatt tagacaattc agcaagggct
     9961 gccaagtgcg tgtcgttggc accttctgga gctgcaatca tgaagaacaa atcagttggt
    10021 tgaccatcaa gacttgcgta gtcgacaccc ttgtttgatt ttgcaaagag aacagtcgct
    10081 tcttttacag ccgcattctt agcgtgtggc attgcgatac cgtcaccaag tcctgttgtc
    10141 gtttgtgctt cacggttcat gatacctgtt ttgaatacgt caaaatccga tacataacct
    10201 ttatcaacca aactagcaat catttcatcg ataactgctt ccttgcttgt cgcttgcaaa
    10261 tcaagcaaca tgacatcttt tctcaaaacg tcttgaattt tcatagtgtt tctacctcaa
    10321 ctttttcata agtttcctta ataaattcga tacttgccaa atcatctgaa aaggctgttg
    10381 ccgtaccgca agcaactccc cattttaggg cttcaattgg gtcttgtgaa cgaacgtatt
    10441 cgccagtgaa tcctgccacc atggaatctc cagctccgac agagtttttc actgtaccct
    10501 tgattggttt ggcaaagtaa gtagctgctg gagtgaccaa caaagcacca tcaccagcca
    10561 tagaaattat cacattctgg gcacccattt ctaagatttt cttagcgtag gtctcgatgt
    10621 ctgcaatgcc attcaattcg acattgaaga ttgcttcaag ttcatggttg tttggcttga
    10681 ccaagagtgg ttggtgagct aatgagtcca gaagtgtctg accttcaaag tcacagacaa
    10741 cttgcgctcc tgcttttttc gccacaggaa tcagcctatt gtagacttcg tttccaagac
    10801 tagctggagc agacccagca aagacaacgg tgtcttcttt ggtcaatcct gctaagacca
    10861 tttctaattc ggccaactgt gtatctgtca caatcggacc caaaccgttg atttctgttt
    10921 cttggtcagc tttgattttc acgttaatac gtgtatcttg atcaacctga acaaaatcag
    10981 tatcaattcc ttcggcaatc aatccatctt tgatgaactg acctgtgaat cccccaagga
    11041 aacccgttgc tgtattcgga tagcccaagc gcttcaaaac acggctaacg ttgattccct
    11101 taccgccggc atacttatcg tcgctctcca tacgattgac actgcctgtt tcaactctat
    11161 caagtcgaac aatgtagtca atagctggat tgagcgtaac tgtataaatc atacctcgat
    11221 taccctcgtt ttctctttta tgatgtccaa caaactgctc tccgaagatt gacagataat
    11281 gttggctttt tcaatagctg caacctttgc aaaggagaat tgaccaattt ttgacgcatc
    11341 agctaaaaca tagctttctt ttgcattgtc tatgatggta cgtttgatgg tcgcttcttc
    11401 aatgtctggc gttgtaaagt agtttttatc aataccattc atccccagaa atgccttatc
    11461 aaaattgagc tgtcgaattt gttccaatgc tactgctcca acagaagcat ctgttgattg
    11521 cttaacaaaa ccacctataa tgattgtttt tatcttgcgt tcaaccagcc tcactgcgtg
    11581 gtgaatagaa ttggtaacga ctgtcagatt ttcctgctga agataatcaa tcagtacacc
    11641 ggttgttgta cctgcatcta aaaaaattac gtctccgtta acaatctgtc gagcagctct
    11701 ttccgcaatc tctcgttttt cttgaacgtt tttgacagat ttttctagaa tagactcttc
    11761 gagttgcaaa ttccctggcg cttcagcacc gccgtgtaca cgacgaagct taccatcgtt
    11821 ttccaactcg tccaaatccc tacgtacagt agactcagat gtatcaagaa cttcgattaa
    11881 ttcctctagc cgaacaaact gttgagagtg tattcgctcc aatataatct gttttcgttc
    11941 tgatttgagt atgttcaaca cctcctgcaa tcgtttacaa aaatcattat acacgatttt
    12001 ctgccattgt caatcatttt ctgtcatttt ctttcaaaaa aactagcaga aatctacgct
    12061 tccactagtt tctcaatcat ttttttcatc atatccattt cttctggacg accaatagat
    12121 aggcgaatat gcccctctaa tttacctgtt ggataatatt tgaaattcca atcctgtgcc
    12181 aaggcttgct gataaaattc ttctgcccac ggtgctttaa acgttacaaa actagcctga
    12241 ctaggtagaa cttgacagtt atctaaccct tgcaagaata aaatcatttc ttctcgcaaa
    12301 ttctgaatta ctcccactgt ttctaaaact ttgtctgtat aattcaatgc aacggctgcc
    12361 atttttgcag tcaagtttga caagctgaat gggggaatga ccttgtccaa ttcataaatt
    12421 aactcttcgc ttcctaccgc aaagccaact cgtaaacctg caagtccaaa agcctttgat
    12481 aaggtccgta aaactaaaac attgtcaaat tgttgcagac gactgatgaa gctttcgaca
    12541 tttgcaaatt caatgtaagc ctcatctaca acgagtagcc ctttaaaaga aatagccaat
    12601 ttctccaaag cttctagtga ataagctact gaagaagggt tgttaggatt ggaaagcatg
    12661 attatcttta ccttcccctc ctctgcacgt gccaacaatt tctctactgg caggacgagt
    12721 gtatcgtttt ttgcttccaa atcaatggtt tcaaaacggc taccatgcat gtgattatag
    12781 gtttcataca tgaagaagtc tggattaacc gtcaagaaag tgtcatcctt ttccataaag
    12841 gtggaaacaa tcatgtgaat aaggtgatca gagcccacac ctactgttac ttgtttggga
    12901 ttcactccaa gataggccgc atatttctca atcaactctg agtaagtgtt atcaccataa
    12961 aaacttaatt gattgacatc caaattttcc aaggtctctt gtggcaaaaa ggaccaatca
    13021 atgatacgat tttcattatt tcccaagtca aacctagcct tttgtttgat tttataaggt
    13081 tggtaatgtt caaatacctt gcgtttcatc atttctcctt ctagcaagaa aaacacagtc
    13141 taatcaactg tgtcttctgc cttgattttt tcaaaaatag ttaactcttc agctgtaaag
    13201 ttgtagtttt ctaacaagtc tggacgacgt tgataggttt tgcgtagact ctcttccaaa
    13261 cgccatttac gaatattttc atggtgaccg ctcattaata catctggaac taccattcca
    13321 cgatactcgt atggtcgggt gtattgagga tattccaata gccctgatga aaaactatca
    13381 tccgtatgac tgacttcctt gccaataatc tctggaatca ggcgaacggt agcatcaatc
    13441 atggtcatag ctgccaactc tccacctgtc aggacataat ctccgagaga aatctcatct
    13501 gttaccaagg tcttaatccg ctcatcatat ccttcatagt gaccgcaaat gaaaatcagc
    13561 tgctcctcct gtgccaattc ttcggcatag gcctgattga aagtacgacc agcgggatcc
    13621 aacaagataa cacgtggctt ggtttgttca atgctatcca tagtatcaaa aattggctgg
    13681 gcacggagca acatgccttg tcctccacca tagggttcat cgtccacatg gcgagccttt
    13741 tcagcattct ctcggaaatt gtggtagttg atttccaaca agcccttgtc acgcgcctta
    13801 cccacaatag agtgttctag aggtgcaaac atctcaggga agagggtcaa aatatcaatt
    13861 ctcatcgtct aggccctcga gcaattccac atcgacacgg ttatttggaa tatcaatgtt
    13921 caagacaaca ggtggaatat atggcaagag caggtctttc ttaccttttc gtttcaccac
    13981 ccagacatca ttggcacctg gctgcaagat ttccttgatt tgtccaatta gctggtcatt
    14041 ttcataaaca tctaaaccta taatttcatg gtaataaaac tcaccgtctt caaggtcgga
    14101 caaattttct tccgctattt tcaaactaaa gcccttgtac ttctcgatat cattgatgtg
    14161 gtacataccc ttgaacttga taatatcaaa gtttttagcc ttgcggtgac tggcaatttc
    14221 aacttccatg acaaactggt ccttgtcatt aaacaaggcc aattttgcac cttttttaaa
    14281 gcgttcttct gcaaaatccg ttacagataa aacacgcatt tctccttgca agccttgggt
    14341 gttaacaatc ttacctacgt taaaataatt cataatttct ccatctgatt ttctatcctt
    14401 ttattttaac atgacgagag tattttcaca agcaaagaaa aaagccggat tgctccggac
    14461 ttcttctagt caatcaactc agacagaggc tttttagttc tgaccgtcag ttcataggga
    14521 aattccaaat aggtcttacc attcttttca tatagatagg ccattttatt ctcaggttga
    14581 aaggtaagtt gataatactc ttctaccccg acgggctggt catggttgct agcatttttt
    14641 ataagatagg gatggatttg ggtaagctcc gatagaaaat cgctcatttc gctgcctttt
    14701 agagttctta tctctccacc atcctgatta ctaattacaa tctggttagc ttgaggaaaa
    14761 cgaatttcag ttttcttcaa aaattgatac ccaaacaaca aggaaataca agataaaaca
    14821 acaccaatca accatctctt tttcatatta gcacccacct tttttagtaa attatatcaa
    14881 gctcagaaaa tctgtcaaga gaaaacatag aaatctattg aatactagca attttcttca
    14941 aacgcaatct tagggcaatc ccgcaaacaa aaatcccgct aatcagacca atccaatagg
    15001 cttctggtcc caggtggaaa aatctttcca ggaaaaaggc aaccggaaag gtcatggacc
    15061 aataagcaac caaaccaagt acaaatggaa cagttgtatc cttgtaacca cgcaaaattc
    15121 cttgaattgg tgctgtatag gcatctgcta attggaacat gagggcaaag cttagaaaat
    15181 gtgacgtcag tcggacaaag tctgtatcat ttccgtagag ataggcaact ttggagcgga
    15241 aaaaatatag gaaagttagc gtcagccctg caaagcctag cgctactaag cgtcccaaac
    15301 agctgtagtt ttttacatct aggggtcggt ttgcacccac ttcataggca accacaatag
    15361 ccaaggctga ggatactgaa gatggaaaag catataacaa ggttgcaaag ttcatggctg
    15421 cctgatgagc cgcgataatc tgagcagaaa attttgccat gtaaaggcca actactgcaa
    15481 aaatagccac ttctgcaaag acttgtaaac cgatgggaag accgattttc aatccttctt
    15541 tcagtaaagc cccatcaatc ggactccact gccaaatctg ataagaagaa atggtcttat
    15601 ttttgcacat gacaaggaca atgactagca acacagccca gtaagctaaa gcagtgccca
    15661 atccagcacc tgctccgcct agggccggta ggcccatttt cccataaatt aagagatagt
    15721 taaagaggct gttaaaggga accaagagca acataaggta catggatagc tgagtcaaac
    15781 cgagggcgtc gaaaaaagaa cgacagacac taaagagcaa gagaggcagg atgccaatgg
    15841 aaataaaata gaggtactgt ctgccaactt gaaaaacaga ttcatcaagt ccaaactgag
    15901 ccaaggcggg aacagcgata aataggatcg agcacaagag taaaatggtt aatccaatgg
    15961 ctagatagac aaactgatga aactcctcac gaatcttatc tctttcacct tttcctaaat
    16021 attgagcgat gattgggacc aacgcagata caatccctgt aagaaatgaa aaaagcggat
    16081 tccaaagact tgttgccatg gaaacacctg ccaaatccat ggtactatac tgaccagtca
    16141 tcatagtatc gataaaggat gcggagtaat tagcaaattg gtaaattaaa atgggcaaaa
    16201 atattttgat aaagagccag agcttctctt tgttatttct tgtttggtac atgcttattc
    16261 tctttcaatt ttatataaat attgtaacaa gaaaaggacc tgcattgcaa gtcctctttg
    16321 actagataga gtcaccattc cagatagagt ttttcacgat aacatagtcc acatgcttca
    16381 agctggtaat gtcacgtcca cctgcataag aaatagatga ctgcaaatct tgctccattt
    16441 caaccagagt atctttcaaa tgtcctttgg cagggagtaa gattttctta ccttctacgt
    16501 ttttataggc acctttttgg tattcagatg ctgaaccgta gtactctttg aatttctcgc
    16561 catcaacttc aattgtttta cctgggcttt cgatgtgacc ggcaaagagc gaaccaatca
    16621 tgaccatgct agcgccgaaa cggatggatt tggcaatgtc tccgtgggta cgaataccac
    16681 cgtccgcaat aattggttta cgggcagctt ttgcgcacca acgaagggct gccaactgcc
    16741 aaccacctgt accaaaacca gtcttaacct ttgtaataca tactttacct ggaccaatac
    16801 caacttttgt cgcatctgca ccagcatttt ctagttcacg aactgcttct ggagtaccga
    16861 cattacctgc aatgacgaac gtttcaggca gttctttctt gatatgttgg atcatcttga
    16921 taacactttc tgcatggcca tgagcaatat caatggtgat aaactcaggg gcatcagctt
    16981 tcaagctagt cacaaactca tattcatatt cttttacgcc aacagagata gaagcgatca
    17041 aaccttgctc gtgcatgcgt ttgataaatg gaatacgacc agcttcatcg aaacgatgca
    17101 tgatgtagaa atacccttct ttggcaagca tttctgctac atcttcatca ataatagtct
    17161 gcatattagc tggaacaact ggtaatttaa agctatattt cccaagagtc actgttgtat
    17221 ctgcctcgga acggctattg atgatacatt tatttggaat gagctgaata tcttcgtagt
    17281 caaaaactgg agtttcgttg aacatgcttc tacctctttt tcttaacatt ggaacagaaa
    17341 aacagtttgt ttttcgtatt ttttccttat tacaagatat agtatagctt attgtttgtg
    17401 ttttgtcaat aaaaaagaaa
//
LOCUS       DATUCG010000042        17291 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.043, whole genome shotgun sequence.
ACCESSION   DATUCG010000042 DATUCG010000000
VERSION     DATUCG010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17291
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.043"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(212..1444)
                     /locus_tag="VCA20_001092"
     CDS             complement(212..1444)
                     /locus_tag="VCA20_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEP1834931.1"
                     /translation="MEKRANYIRRKRYASYRTKMHLFFICGLATIILAIIIVSEHLVP
                     RLNQASESMNSDNSVRIMAHGDLLYHLPILRGAEQPDGSYDFSENFTYVKPWIEQADF
                     AIADFEGTISPDVELAGYPLFNAPAIVANNIHDAGYDLVDLAHNHILDSHLSGLVSTV
                     NTFRDAGVDTVGVYAEGNRATAPLYIREVNGIRIAVLAYAYGFNGMEALLSQEEYDAY
                     LSDFDKEKMQAEIERAEKEADITIVMPQTGVEYQLEPTEEQTSLYHQMIDWGADIVLG
                     GHPHVVEPAEIIEKDGQRKLIIYSMGNFLSNQRIESMDDTPNSQWTERGVLMDIRIQK
                     ENGQTSIRTAQAHPTWVNRISQGTYSQDGYERFSYQTYVLEDWVAGGQYYGQLDLDTQ
                     ARVDTAYQEMKDFVNLVW"
     gene            complement(1779..2717)
                     /locus_tag="VCA20_001093"
     CDS             complement(1779..2717)
                     /locus_tag="VCA20_001093"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEP1834932.1"
                     /translation="MVSTATTLGGFTFDNCLMNAAGVWCMTKEELDAVKNSKAGTFVT
                     KTATLDYRAGNPEPRYQNVPLGSINSMGLPNQGLAYYLDYLLELQETEPERTFVLSVV
                     GMSPDETHEILRTVEASDFKGVTELNLSCPNVPGKPQIAYDFETTETILQEVFTYFTK
                     PLGIKLPPYFDIVHFDQAAAIFNQFPLKFVNCVNSIGNGLYIKDESVVIKPKNGFGGI
                     GGEYIKPTALANVHAFYQRLKPEIQIVGTGGVLTGRDAFEHILCGASMVQVGTTLQKE
                     GVAAFERITAELQAIMAEKGYETIEDFRGKLQHLDD"
     gene            2940..4286
                     /gene="gdhA"
                     /locus_tag="VCA20_001094"
     CDS             2940..4286
                     /gene="gdhA"
                     /locus_tag="VCA20_001094"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEP1834933.1"
                     /translation="MSNAKAYIQASFEAVKARNPHETEFLQAVEELFSTLEPVFEAHP
                     EYIEENILARIVEPERIISFRVPWTDKDGNVQVNRGYRVQFNSAVGPYKGGLRFHPTV
                     NQSILKFLGFEQIFKNVLTGLPIGGGKGGSDFDPKGKTDAEIMRFCQSFMTELQKHIG
                     PSLDVPAGDIGVGGREIGYMYGQYKRLRQFDAGVLTGKPLGFGGSLIRPEATGYGLVY
                     FTDNMLAANGKSFKDQTVLISGSGNVAQYAVQKATELGAKVISVSDSNGYIIDETGID
                     FDLLVDIKEKRRARLTEYAAEKATAKYFDGSVWNYDGKADIALPCATQNEINGEQAAA
                     LVKNGVYCVAEGANMPSDLDAIKVYKENGVLYGLAKAANAGGVAVSALEMSQNSLRLS
                     WAREEVDGRLKDIMANIFNTAKETAEKYDLGTDYLAGANIAAFEQIADSMIAQGLV"
     gene            complement(4522..5109)
                     /locus_tag="VCA20_001095"
     CDS             complement(4522..5109)
                     /locus_tag="VCA20_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HEP1834934.1"
                     /translation="MQTKDFVVENGQMYYKKKPLHKQPLFWTTIAGAVLSFILGVTCF
                     IFMIGLSSANFDNWDTGSDGYIDETVEYTEYQVGDSVDFSNGLHVTVTSMGKDDSIEL
                     VDSYYSTAYVVEMEVENSTAKELYFDEYYFSLMDPVSQIPFTLDLRTYDVNLVEKLKP
                     GEKVQVKLIYGIDNETNFGFTYEDAMWTELVAEGI"
     gene            complement(5120..5968)
                     /locus_tag="VCA20_001096"
     CDS             complement(5120..5968)
                     /locus_tag="VCA20_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEP1834935.1"
                     /translation="MNDFITKYLSQFNIEAIVTAGLNKVLSLVLLFVAFYIIKKIAKA
                     SVKKVLVPSLKVSTQDIGRQKTISRLVESILNYLLYFILIYCILSILGLPVSSLLAGA
                     GIAGVAVGLGAQGFLSDLVNGFFILIERQFDVGDVVKLTNGPITISGTIVSMGIRTTQ
                     VRDADGTLHFIPNRNILVVSNQSRGDMRAQIDIPLKFNTDLEQVYRVIEEVNLREISN
                     FDQITGVTVLGPQNAISGQFVFRVNLFVANGQQNKIYHQFFGFYQDALRQAGIDLPSS
                     GPTVLK"
     gene            complement(6036..7370)
                     /locus_tag="VCA20_001097"
     CDS             complement(6036..7370)
                     /locus_tag="VCA20_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEP1834936.1"
                     /translation="MLELFKAINNLVWGPPLLLLLVGTGVYLTLRLGVFQIGKLPTAF
                     RLIFSSDQSGQGDVSSFAALCTALAATVGTGNIVGVATAITTGGPGALFWMWVAAFFG
                     MATKYAEGFLAIKYRTKDANGQAAGGPMHYITLGMGKKWKPLAVFFAISGVLVALLGI
                     GTFSQVNSITASLETSFGLAPQLVNIVTAISIAFFIFGGIEKISDVSTKVVPFMAILY
                     ILASITVLAVHWDQLLPTLALVFKSAFTPAAAMGGFVGATVKEAIQRGIARGVFSNES
                     GLGSTPIAAAAAKSDNPVEQGLISMTGTFIDTLIICSLTGLSILVTDQWTTEGLAGAP
                     LTQAAFATVFGNTGSIALTISLVLFAFTTILGWSYYGERCIEFLFGTKAILPYRLVFV
                     AMVALGGFLKLDLIWTIADIVNGLMALPNLIALLALSPVIIKETRQYFAKRK"
     regulatory      complement(7476..7580)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(7793..8425)
                     /locus_tag="VCA20_001098"
     CDS             complement(7793..8425)
                     /locus_tag="VCA20_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1834937.1"
                     /translation="MFSVQSIQKKFGKRSLFSDVTLDLQAGKVYALIGASGSGKTTLL
                     NILAKLESYDAGDILYKGKDLKKLKPHLFFREELGYLFQNFGLLESQTIRENLDLGLI
                     GQKLSKDERKSRQEAALDAVGLSYLNLSQPIYELSGGEAQRVALAKVILKNPPLILAD
                     EPTAALDPKSSQEIMDILLSLKSPDRLIVIATHNPLIWERADLIIKMEDL"
     gene            complement(8427..10412)
                     /locus_tag="VCA20_001099"
     CDS             complement(8427..10412)
                     /locus_tag="VCA20_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HEP1834938.1"
                     /translation="MKGKFVLVSTIILAVFMIWLGVSQKETMLVHYYPSVTALSVSED
                     VTYSDVRQRLEEYSQQTDSVIARRVIEPSKSGGRTFSYDNFSQTPLPRGLEEFQASEK
                     VESALLTKYFIFQGKATVEELRSLLVSLGFDEVQVRKPSTIATLLAFLTQGGQFLAVL
                     VFLITYMALVVIANVRQLRTAGIRLIAGDSRWHLFLLSLQENTKEIALTIPFAVLPAV
                     GLACLIGLDGYSVYYLVAALVGYHFLLGLIVLFFAATFTLGIRTYHFLPLLKGKMPLQ
                     GILTIMVMGQMLALLVVSFGVVQTVYYSGIWQEYQVGAQQWENEGDYYSLAWNISADG
                     RSGLNSPENWYPLLKQALEEDGALFVKSNLNAYLMGSQLEDGTRLDSYHPAGNTLYVS
                     PNYLRIQDVNLAEGEAALPLQEGEFQLLLPEKLRPESDTYLHLYQDYINRMVIPADQA
                     RSATIKGKVAYLKNGQKQFIYNHRSGQHVQYLTDPILVVLTPSSLGKESADFWLNEVD
                     SGILFKNRDKLLRELKARNLFQFVNEVKSSSSLFTELLDRIWIETISTSMGAILGIVT
                     SIVLFNTMNLLYFAEFKREMFIKEIAGMDFLGIHKKYLTFQLLSLLLALGASMVVTGH
                     IFISSISFALFMVNALCLLRWQARKEGAWNVRILKGA"
     gene            complement(10432..12417)
                     /locus_tag="VCA20_001100"
     CDS             complement(10432..12417)
                     /locus_tag="VCA20_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HEP1834939.1"
                     /translation="MKKVFTYLSTFSICLFCMWLVSLQLDSLIYHYYPSVSILSRGEE
                     VTYEEVSDALTNLAEKENSLIAKTITETDDSGRTTTSYDVFGKGELPQGLVKADRLVA
                     SQANLAENYYVVKGNLDIKAVENVLQSLGLVDSYIYRPTLLNSLSLFLGRGAPSLGLI
                     IFILTNLALTVVGQIVYLRSAGIEILSGVRSWQIASRFLRVDGRDIALATVGALVFGL
                     IAILILGWPMVYYRFVAIGIAFYACLLFFLSLSTSIGFMIALNISQLQKLIKGALPLK
                     AIFAILLVGQCLAVCIISIGASRTLTYVGVLHTLTIGEKAWQERQDVFSLSLNREAFI
                     ADEAMAQQQRIAWSHVILESYAKNNVMLVQHHLRHNTIKEHTIIESSKEIPLERVLYV
                     TPNYIQSEGGLYDFNQLEEIKQLGKGEIALLLPKSLQNDASVYQAYFEDMVGKLLADG
                     ERSLSLHSNVYYISDEKRWFIYNHTPINYEQFLQAPLIVVLSPESFEETSYFWENALP
                     DFVFFKDKEMLEQLLDRYNLQNTIGSLLSSKQHYNTLRKNVQLEILMTLSTTILGIFT
                     SILLFNTMNLLYFETFKREIAIKRIAGMRFIELHGSYLGEQVGSALIGMGLAMFMTKS
                     VIVSLGVVVALLINSWMLLNNQAKREEKIQLSVLNGR"
     gene            complement(12469..12768)
                     /locus_tag="VCA20_001101"
     CDS             complement(12469..12768)
                     /locus_tag="VCA20_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000911560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEP1834940.1"
                     /translation="MFKTSRKLAFLLVLLTMSFAVPVLAISVEGGEWNYGGHHDPSNW
                     GAYSSYYHARERHYARVSSTQRPGKSSKVYAGPGGVAYAFINTDIGEHVDFGYGF"
     gene            complement(13122..13979)
                     /locus_tag="VCA20_001102"
     CDS             complement(13122..13979)
                     /locus_tag="VCA20_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP1834941.1"
                     /translation="MILSKRFKNRRKELGFTQKELAEGICEQSLISRVEKLGVAPTSD
                     ILFALSQRLQVSMDYFFDESVSDKAPDITVFKRLVDKALFTRSYDQLAYLVEAEKQKE
                     VVHSQESSEYLTYLACIVDFHHYHKEDIAIVRMEELSHRISKKSSFYLDVYSSLANFY
                     ALTSRDEDLESLYEEISEKLSHLDITDTDRFHKYIKIRYNHAHYLFKRKRQSQAIDEL
                     TDLIEILRDKKSCYLLADTLCLIANVGEGFLSKDEILSYYREAECLFKFFGPQNSYLN
                     LKEYLSKDI"
     gene            complement(14239..15177)
                     /gene="msrB"
                     /locus_tag="VCA20_001103"
     CDS             complement(14239..15177)
                     /gene="msrB"
                     /locus_tag="VCA20_001103"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEP1834942.1"
                     /translation="MKEIYLAGGCFWGMEGYFSQIDGILDTSVGYANGQVETTNYQLL
                     KQTDHAETLYLAYDETRINLRKILLYYFRVIDPFSVNQQGPDKGRQYRTGIYYTDEAD
                     LPTIEQVMTEQSQLFGGRPLAVEVEPLAHYIPAEDYHQDYLKKNPQGYCHIDLGQAKI
                     PLIDVADYQKPDQQVLKDSLTDLQYQVTQEAATERPFENEFWNSDQAGIYVDITTGEP
                     LFLSTDKFDSGCGWPSFTKPISKEVATYFQDRSHGMNRIEVRSRAGHAHLGHVFDDGP
                     RDKGGLRYCINSAALRFIPREEMEEAGYGLFLDLLK"
     gene            complement(15217..16746)
                     /locus_tag="VCA20_001104"
     CDS             complement(15217..16746)
                     /locus_tag="VCA20_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEP1834943.1"
                     /translation="MSTISYKPLNLYRQFKTAAQEFPNVAIYFDQPYASFPELVLENT
                     YQTVFEAIQKRAAQFAAAGIGYGDKVVLYKSPAFDTYLLAVAVTALGAVPVMISYHLP
                     SSNLDVFAERLEKSFIVYDQETEERVAGMTRTDLVTKIFLPQVLVQEAVAFEENLLPE
                     DVIQYMTHTSGTTGVPKLICHTAQTMGWRVAWQQTIFDKMTERGLLAFHISPVHSRYN
                     IGVSSAIGLGFPLYPLSSARKDDVERALALHRPSALETHPNNFVQWARLAKEKPEVFS
                     SIRYYHSTFDAINIGTLRAFLEASKEQNPVFMQVYGQSECGPMILRYHRLENLGTVSG
                     RDMGIGLEGYTEARITDAQGNPLPAGENGHIQFLSKGRAVTYYKEDARFQDNVYGTWW
                     DSGDYGCMTPEGTLLLKDRQVDLIEHIDSNLALEDLLLDKLDFLSEVVIIRDVNGAPQ
                     PIIALAEDAEMNWEAWWTMVADLPLLKEPILMAYDDIPRTATMKVQRLKMEAEMKEKN
                     L"
     gene            complement(16883..17197)
                     /gene="trxA"
                     /locus_tag="VCA20_001105"
     CDS             complement(16883..17197)
                     /gene="trxA"
                     /locus_tag="VCA20_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEP1834944.1"
                     /translation="MVQVITDANFEVETQEGVVLVDFWAPWCGPCRMQAPILEQLADE
                     VDEDELRIYKMDVDENPNTARQFGIMSIPTLLFKKDGQVVKQVAGVHTKNQIKAILAE
                     IG"
BASE COUNT         5224 a         3935 c         3177 g         4955 t
ORIGIN      
        1 taactaccgt tggaaacata caatttaacg tttaacaaat caagggtgtc tccataaaac
       61 caaagtattt ctgataccca tttccttcta tctataatca gtaaaaaacc ttggaaacaa
      121 cttatcccaa ggctttcttt gtattctatt gtatcaattt ccttattccg atttctgtag
      181 caattaatcg aactgtcatc tttctctctc ctcaccatac caaattgaca aagtctttca
      241 tttcttgata ggctgtatcc acacgcgcct gtgtatctag atccaattgt ccataatatt
      301 gaccaccagc gacccagtct tccaatacat aggtctgata gctaaaacgc tcatatccat
      361 cttgtgaata cgttccctga gagatacgat tcacccaggt tgggtgcgct tgggctgtcc
      421 gaatacttgt ctgcccattc tctttttgaa tccttatatc catcaagaca ccccgctcgg
      481 tccactggga atttggtgta tcatccatgc tttcaatacg ctgattggac aggaaattcc
      541 ccatcgaata gataatcaat tttctctgac catctttttc aataatctct gcgggctcga
      601 ctacatgcgg atgcccacca agaacgatgt ctgctcccca atcaatcatt tgatggtaca
      661 aactggtctg ttcctccgta ggctccaact ggtattccac acccgtctga ggcatgacga
      721 tggtaatatc tgcctctttc tcagctcgct caatctcagc ctgcatcttt tctttatcaa
      781 aatccgataa ataggcatca tattcctcct gactcagcaa agcctccatc ccattgaaac
      841 cgtaagcata ggctaggaca gctatacgga taccattgac ttcccgaatg tagagtggtg
      901 cagttgcacg attgccttcg gcgtagacac cgactgtatc cacacctgca tctctaaatg
      961 tattgacagt ggaaaccaat ccagaaagat gtgagtccaa gatatgattg tgggctaagt
     1021 ccaccaaatc gtagcctgca tcatggatat tgttggcgac aatggcaggc gcattaaaaa
     1081 gtgggtaacc tgctaattcg acatctgggg aaatggtccc ttcaaagtct gcgatagcga
     1141 aatcagcctg ttctatccag ggctttacgt aggtaaaatt ctcagaaaaa tcgtaacttc
     1201 catctggctg ttctgctcct cgaagaatag gcaagtgata gagcaaatcc ccatgagcca
     1261 tgatgcgaac agaattatct gagttcattg attctgatgc ctgattcagc ctaggaacta
     1321 ggtgctcgct tacaataata atcgccaaga taatagttgc taagccacaa ataaaaaaca
     1381 aatgcatctt tgtccgatag gacgcatatc gttttctgcg aatatagttc gcccttttct
     1441 ccataccttt accctcatac tccctactta agaacctttg ttcaaaatac agtcagtagt
     1501 ccctcaaaaa actatttctt ccataaaagt acatcactta taaatagagg ttctaatatt
     1561 agtatacgac atttttgcta aaaaatctaa ataaaatcag aattccatct cacaaatata
     1621 tgtttctgac tatgtattcc attcgtcaaa cgattatcag accgatcttc acagaccatt
     1681 taaaaagagc taacactatc ccctcctaac tccctaaaag actatctggg gggaacgata
     1741 caatgttagc ttctttatta taccttcaac agatgttctc aatcatctaa gtgctgtagc
     1801 ttgccacgaa aatcttcaat ggtctcgtag cctttttcag ccatgatggc ctgcaattca
     1861 gccgtaatgc gttcaaaggc tgccacacct tccttttgca gggttgttcc aacttgaacc
     1921 atgctggcac cacagagaat gtgttcgaag gcatcccgac ctgtcaagac gccaccagtc
     1981 ccaacaatct gaatctccgg tttcaagcgc tggtaaaaag cgtggacatt ggcaagagca
     2041 gtcggcttga tatactctcc accgatacca ccaaaaccat ttttaggctt gatgacaact
     2101 gactcatcct tgatatatag accatttcca atcgaattga cacagttgac aaacttcaaa
     2161 ggaaactggt tgaaaatcgc tgctgcttgg tcaaaatgca caatatcgaa ataaggtggt
     2221 aacttaatac cgagtggttt ggtaaagtaa gtgaagacct cctgcaagat ggtttctgtc
     2281 gtttcaaaat cataggcaat ctgcggtttt ccagggacat tgggacaaga aagatttaac
     2341 tctgtcaccc ccttaaaatc actggcttct acagttctca aaatttcatg agtctcatca
     2401 ggcgacattc caactacaga taaaacaaat gtgcgttctg gctccgtttc ctgcaattct
     2461 agtagataat ccaaatagta agccaagccc tgatttggca agcccatcga attgatagaa
     2521 ccaagcggaa cattctgata acgtggttct ggattacccg cgcggtagtc caaggttgct
     2581 gtttttgtca caaaagtacc cgccttcgag ttcttaaccg catccaattc ttccttggtc
     2641 atgcaccaaa cccctgccgc attcatcaaa caattatcaa aggtaaatcc accgagtgtc
     2701 gttgctgttg aaaccatatt atcctccata taggtcattc tttcttgatt atatcataag
     2761 aaacaaacgg attttctcca tttactacta aggttcggat tttgcaataa aaatttatta
     2821 atttttttaa aattcttaca ttttcttctt tacatttaaa aatcatggtg ctataataat
     2881 tatcagataa attaaattat cagacaattt aataaataaa aaagaaatca ggtgatccca
     2941 tgtcaaatgc caaagcttac atccaagctt cttttgaagc agtcaaagcc cgcaacccac
     3001 atgaaacaga attcctccaa gctgtagaag agctcttctc tacacttgag cctgtttttg
     3061 aagcacaccc agaatacatc gaagaaaaca tcttggcccg tattgttgag cctgagcgta
     3121 tcatcagctt ccgtgttcca tggacagata aagatggaaa tgttcaagtc aaccgtggct
     3181 accgtgttca gttcaactca gctgtaggtc cttacaaagg cggtcttcgc ttccacccaa
     3241 ctgtaaacca atccatcttg aagttcctcg gttttgagca aatcttcaaa aacgtcttga
     3301 ctggtcttcc aatcggcggt ggtaaaggtg gttcggactt tgatccaaaa ggaaaaactg
     3361 atgctgaaat catgcgcttc tgccaaagct tcatgactga attgcaaaaa catatcggac
     3421 cttcacttga tgtccctgct ggtgacatcg gtgtcggtgg tcgtgagatt ggttacatgt
     3481 acggtcaata caaacgcctc cgccagtttg atgcaggtgt cttgactggt aaacctcttg
     3541 gctttggtgg ttcattgatc cgcccagaag caactggtta cggtttggtt tacttcactg
     3601 ataacatgtt ggcagcaaac ggtaaatcct tcaaagacca aacagtcctt atctcaggtt
     3661 ctggtaacgt tgcacaatat gctgttcaaa aagcgactga acttggtgca aaggttattt
     3721 ctgtttcaga ctcaaatggt tacatcattg acgaaactgg tatcgacttc gacctcttgg
     3781 tggacatcaa agaaaaacgc cgcgctcgtt tgacagaata cgctgcagaa aaagcaactg
     3841 ctaagtactt cgatggttct gtatggaact acgacggaaa ggctgatatt gcccttccat
     3901 gtgcgactca aaacgaaatc aacggtgagc aagctgctgc ccttgtgaaa aatggcgttt
     3961 actgtgtggc tgaaggtgcc aacatgccat ctgaccttga tgctatcaaa gtttacaaag
     4021 aaaatggcgt tctttacggt cttgcaaaag ctgccaacgc tggtggtgta gccgtatctg
     4081 cccttgaaat gagccaaaac agccttcgtc tttcatgggc tcgtgaagaa gtagacggcc
     4141 gtcttaaaga catcatggca aatatcttca acacagctaa agaaactgct gaaaaatacg
     4201 accttggtac agactacctt gcaggtgcta acatcgcagc ctttgaacaa attgcggata
     4261 gcatgattgc ccaaggtttg gtataagctt acttttagtt tactaaacta taataaaaac
     4321 gcccccaaaa ttgacgtgct aattttgggg gtatttttca ttaaaagaag aggagaacag
     4381 tggataaaca tgttctcctt tttaaaatgt aaaaataaca gataagtcgg atatagtaca
     4441 attaccaagc cgaacaatct gaaccaaggg gcgttataaa cccgcaaaat tgtgcaatat
     4501 ttaaaacttt ttctacagca attatatacc ttctgcgact aattccgtcc acatggcatc
     4561 ttcgtaggta aaaccaaagt ttgtttcatt atctataccg tagataagtt ttacttgtac
     4621 tttctctcct ggcttcaact tttcaactag attcacatca taggtccgca aatccagggt
     4681 aaagggaatc tgactaacgg gatccatgag actgaaatag tattcatcaa aatagagctc
     4741 ttttgcggtc gaattttcaa cttccatttc aacaacataa gctgtgctgt agtagctatc
     4801 cactagttct atgctatcgt ctttgcccat agatgttact gttacatgaa gaccgttaga
     4861 aaaatcaact gagtcaccaa cttgatactc tgtgtattcc acagtctcat caatgtatcc
     4921 gtcactacct gtatcccaat tatcaaaatt tgctgagctt aggccgatca taaaaataaa
     4981 acaggttact cctaggatga aggagagaac ggcaccagca atggttgtcc aaaagagagg
     5041 ctgtttgtgc aagggtttct tcttataata catctgccca ttttcaacga caaaatcttt
     5101 tgtttgcata ataaactcct tattttaaaa cagttggccc acttgaaggt aaatctattc
     5161 ctgcttgacg cagagcatct tgataaaaac caaagaattg atgatagatc ttgttctgtt
     5221 gaccgttggc tacgaaaaga ttaacacgaa atacaaactg tcccgaaata gcgttttggg
     5281 gacctaatac agtcacacct gtaatctggt caaaattgct gatctctctc aaattgacct
     5341 cttctattac gcgatagact tgctccaaat cggtattaaa tttgagcgga atatcgattt
     5401 gagcacgcat gtcccctctt gactggttgg acaccactag aatgttgcga ttgggaataa
     5461 aatgcaatgt cccatctgca tcacgtactt gtgttgttcg gattcccatg ctaacgatgg
     5521 ttcctgaaat ggtaatgggc ccgttggtta acttaaccac atctccgacg tcaaactgtc
     5581 gttcaattaa gataaagaat ccattgacca agtctgagag aaaaccttgt gcgcccagcc
     5641 caacagctac cccagcaatc ccggctcctg caagaagact ggataccggt aaaccgagga
     5701 tactcaaaat acagtagata aggataaagt agagtaggta atttaggatg ctttccacca
     5761 gacgactgat ggtcttctga cgaccaatgt cttgtgtaga tacctttaga gagggaacca
     5821 aaaccttttt cacagaagct ttggctatct tcttgataat gtaaaatgca acaaagagta
     5881 aaacaagcga taaaacctta tttagacctg ctgtcacaat ggcctcaata ttaaattgag
     5941 acaaatactt ggtaataaaa tcattcatga gataattata gcagaaaaca tcttaaaaga
     6001 acaaaaagac agccaaacga ctgtccttct tttccttatt tcctctttgc aaaatactgg
     6061 cgtgtctcct tgatgatgac aggagagaga gctagtaagg cgatcaagtt tggtagggcc
     6121 atgagaccat ttacaatatc ggcaatggtc caaatcaaat ctagtttgag gaaaccaccc
     6181 aaggcaacca tagctacaaa gaccagacgg taaggtaaga tggctttggt tccaaataga
     6241 aactcaatac agcgttcacc atagtaagac catccgagaa tagttgtaaa agcaaaaagt
     6301 accaaactaa tcgtcaaggc aatagatcct gtattgccaa aaactgttgc aaaagctgcc
     6361 tgagtcagag gagcaccagc taatccttcc gtcgtccact gatccgtcac caaaatcgaa
     6421 agccctgtca gactgcagat aatcaacgtg tcgataaagg tacctgtcat ggaaatcaat
     6481 ccctgctcca cagggttatc cgacttggct gcagctgccg caatgggagt agaacccaga
     6541 cctgattcat tggagaagac tccgcgagca attccgcgtt gaatggcttc ttttaccgtt
     6601 gcccctacaa aaccacccat tgcagctgct ggagtgaagg cagatttgaa aactaaggcc
     6661 agggttggca agagttgatc ccaatgcaca gccaagacag taatacttgc taagatatac
     6721 aaaatcgcca tgaaaggaac aactttggtc gaaacgtcag agattttttc aatcccacca
     6781 aagataaaga aagcaattga aatagctgtc acaatgttga ccaactgggg agctaatcca
     6841 aaactcgttt ccaaagaagc agtgatggaa ttaacttgcg aaaaagttcc aatccccaaa
     6901 agagcaacca aaacacctga aatagcaaag aagactgcta gaggtttcca ttttttgccc
     6961 atccccaagg tgatatagtg cataggcccg cctgctgctt gaccgttggc atccttggtt
     7021 ctatatttga tggccaaaaa gccttccgca tacttagttg ccattccgaa aaaggccgca
     7081 acccacatcc agaaaagagc cccaggacca cctgtagtaa tagctgtcgc aactccgaca
     7141 atatttcctg taccaactgt cgctgccaaa gctgtacaaa gtgccgcaaa gctggacaca
     7201 tctccctgtc cagactggtc actggagaaa atcagacgaa aagccgtcgg caatttgcca
     7261 atctgaaaca ctcccaaacg aagagtgaga taaacacccg ttccaaccaa tagtaacaag
     7321 aggggcggtc cccaaacaag attgttgata gccttaaata gttctaacat agttcctcct
     7381 aagcgtttct aatccgataa agctgccgga aaaacaaaaa gaggctgaac acttcaacct
     7441 ctagaatcaa cgtgaaaata tagaaaaagc ctacttttca acgctctgtc cttttacctg
     7501 agagtttcag tagctctact tttccaagca ggactacctt gccccttcgg tgccaaaatg
     7561 gtctctccag agttccgtcc attgaacagt cagtttccct tctacccaat atcaaccggt
     7621 tattcagttt tatctagttt tattttaatc taattttacg aaaaaatcaa gaatattttc
     7681 tgaaactcaa tgaaaatcaa aagtagccta ggaaacgaat ccgaagatag aactggagtt
     7741 catcaaggta agttgacaac ggataatttt gattttcgaa gagtatgaat ttttacaagt
     7801 cttccatttt tatgataagg tctgctcgct cccaaatcaa aggattatgg gtcgcaatca
     7861 caattagccg atctgggctt ttcaaagata acaaaatatc cataatttct tgggagctct
     7921 taggatctaa ggcggccgtc ggctcatctg ctaaaatcaa tggaggattt ttcaggatga
     7981 ctttagcaag agctacccgc tgggcttccc ctcctgagag ttcatagata ggctgggaca
     8041 ggttcagata agacaaaccc actgcatcaa gtgccgcttc ctgcctcgat ttcctttcgt
     8101 ctttggacaa tttctgacca atcagtccca aatccagatt ttctctgatt gtctgacttt
     8161 ctaaaagacc aaaattctga aagagatagc ctaattcctc cctaaaaaag agatggggct
     8221 tcaacttttt caaatccttt cccttgtaga gaatgtcgcc tgcatcatag gactccaact
     8281 tggctaaaat attcagcaag gtcgtttttc cgctaccact ggcacctatc aaggcataga
     8341 cctttccagc ctgcaagtct agcgttacat cagaaaacaa gctgcgcttc ccaaatttct
     8401 tttgaattga ttggacagaa aacatactag gctcctttca aaatacggac attccaagct
     8461 ccttctttcc tcgcctgcca cctcaataaa cagagggcat tgaccataaa caaggcaaaa
     8521 ctgatgctac ttataaaaat gtgacccgtt actaccatgc ttgctccaag agctaacaag
     8581 agggaaagca gttggaacgt taggtatttc ttatggattc ccaagaaatc catccctgca
     8641 atttccttga tgaacatctc acgtttaaat tctgcaaagt atagaagatt catggtatta
     8701 aacaggacaa ttgatgtcac aatccctaaa atggcaccca tagatgttga aatggtctct
     8761 atccaaatac gatctaaaag ttccgtaaag agggaggaac tacttttcac ttcattgaca
     8821 aactgaaaca gatttcttgc cttcaattcg cgcaaaagtt tatctctatt tttaaataag
     8881 ataccgctat ctacttcatt caaccaaaag tcagcagatt cttttcccag acttgacggt
     8941 gtcagcacaa ctaaaatagg gtctgtcagg tattgtacat gttgaccaga acgatgattg
     9001 tagataaact gcttctgccc attttttaaa taggcaactt ttcccttaat ggtagctgag
     9061 cgtgcttgat cagcaggtat aaccatacga ttgatataat cttggtaaag atggagataa
     9121 gtatcgcttt cagggcgcaa tttttcaggc aacagcagtt gaaattctcc ttcttgtaga
     9181 gggagcgctg cttctccttc tgctaagttt acatcctgta tccgcaaata atttggacta
     9241 acatagaggg tgtttccagc tggatggtag ctgtcaaggc gtgtcccatc ttccagttgg
     9301 gagcccatta aataggcatt caaattactt ttgacaaaga gcgcgccgtc ttcctccaaa
     9361 gcttgcttta gtaaaggata ccaattttca ggcgaattta gtcctgagcg accatccgca
     9421 gaaatattcc aagctaaact atagtaatcc ccttcattct cccactgctg ggcacctact
     9481 tggtattcct gccaaatccc tgaataatag acggtttgaa ccacaccaaa tgacaccacc
     9541 agtagagcca gcatttgtcc cataaccata atggtcagga ttccttgcaa gggcatcttc
     9601 ccttttaaca agggtaaaaa gtgataggtc cgaatgccca aggtaaatgt agcagcgaaa
     9661 aaaaggacaa tcaaaccaag caagaaatga tagcccacta gagctgcgac cagatagtag
     9721 acagaatagc catctaatcc aatgagacag gccagtccaa ctgctggaag aaccgcaaat
     9781 ggaatagtta gagctatctc tttcgtattt tcctgtagag atagtagaaa taaatgccaa
     9841 cgtgaatctc ctgcaatcaa acggatacct gctgtacgca actgccttac attagcaatc
     9901 acgaccaaag ccatataggt aatgagaaag accagtacgg ctaagaattg accaccttgt
     9961 gtcagaaatg ctagtaaagt agcaatagtc gaaggcttcc gaacctgaac ttcatcaaat
    10021 ccaagcgaaa caagaagaga ccgaagctcc tctaccgtcg ctttcccttg gaaaataaaa
    10081 tacttggtta aaagtgcaga ctccactttt tctgatgcct gaaattcctc tagtcctctt
    10141 ggtaggggag tttgactaaa attatcataa gaaaaggtac gtccacctga tttacttggc
    10201 tcaatcactc gtctggcaat gacactatcc gtctgttgag aatattcctc taatcgctgt
    10261 cgtacatctg agtaagtcac gtcctcagat accgacaagg cagtcacaga aggataataa
    10321 tgaactagca tggtctcctt ttgactgacg cccaaccaaa tcataaagac ggctagaatg
    10381 atagttgata caagtacaaa ttttcccttc attctaactt cacctcatta actatctgcc
    10441 atttaataca ctcaactgaa ttttttcttc cctcttcgct tgattgttca gtaacatcca
    10501 actatttatt aacaaagcca caactactcc taggctaaca ataactgact ttgtcataaa
    10561 cattgccaaa cccatcccaa taagagcgga gccgacttgt tctcctagat aagaaccatg
    10621 caattcgata aaacgcatcc ctgctatccg cttgatagct atttctcttt tgaatgtctc
    10681 aaagtataga agattcatcg tattgaacaa tagtatggaa gtgaatattc caaggattgt
    10741 cgtagatagg gtcataagaa tttcaagttg aacatttttt ctcagggtgt tgtaatgttg
    10801 cttgctagat aacaagctac ctattgtgtt ttgtaaattg tatctgtcca acagttgttc
    10861 cagcatctct ttatctttga aaaatacaaa atctggtaac gcattttccc aaaaatagga
    10921 cgtttcttca aaactttcag gtgatagaac aacaatcaac ggagcctgta aaaattgctc
    10981 ataatttatc ggagtatgat tgtaaatgaa ccatcgcttc tcgtctgaaa tataataaac
    11041 gtttgaatgc agacttaggg atctttctcc atccgcaagc aattttccta ccatgtcctc
    11101 aaaatacgct tggtaaacac tcgcatcatt ttgcaaactc tttggtaata ataatgcaat
    11161 ttctccttta cctaattgct ttatctcttc cagctgattg aaatcgtaca ggccaccttc
    11221 tgattgtata taattcggcg tcacatacag caccctttct agaggaattt ccttgctgga
    11281 ttcaatgatg gtatgttctt ttattgtgtt atgccgtaaa tggtgctgga ctaacattac
    11341 attatttttc gcatatgatt ctaaaattac atgagaccaa gctattcttt gttgctgggc
    11401 catcgcttca tcagctatga atgcttcacg attcaaagac agactaaaaa catcttgtct
    11461 ttcttgccaa gccttttctc ctatcgttag tgtatggaga actccaacat atgtcaacgt
    11521 tcgactagca ccaatagaga tgatacaaac cgctaaacac tgacctacta gtaaaatagc
    11581 aaaaatcgcc ttgagaggca aagctccctt aattagcttt tgtaactgag aaatgtttaa
    11641 tgcaatcatg aatccaatac ttgtcgataa agataggaaa aaaagcaaac aagcataaaa
    11701 ggcaataccg attgctacaa agcgataata taccattggc caacccaaaa ttaaaatagc
    11761 aatcagtccg aaaacaagcg cccctactgt agccaatgct atatctcttc catccactct
    11821 taaaaaacga ctagcaatct gccaactcct cacacctgaa agtatctcta tccctgctga
    11881 ccgcaagtag acaatctgcc caactactgt caatgcaaga ttggtgagta taaaaataat
    11941 aaggcctaag ctcggagctc ctctaccaag aaatagactt aaactgttta atagggtcgg
    12001 tctataaata tagctatcca ccaatcctag tgactgtaag acattttcta ccgctttgat
    12061 gtccagattc ccttttacaa catagtaatt ttcagctaag ttggcctgac tagctactaa
    12121 cctatccgct ttgaccaagc cctgcggaag ctcccccttc ccaaaaacat cataactggt
    12181 agtagttcgc ccactatcat ctgtttctgt tattgtctta gcaattagac tattttcctt
    12241 ttcagctaaa tttgtcaggg catccgacac ttcttcatag gtcacttctt cccctcgact
    12301 taatatggaa accgatggat agtaatgata aattaaagaa tcgagttgca gactcactaa
    12361 ccacatacag aagagacaga tgctaaaagt agataaataa gtaaatactt ttttcataac
    12421 gaaccccatt tgcacaaaaa ggaggtggac tccaccacct ccctatcatt agaacccgta
    12481 accaaaatca acatgttctc caatatctgt attaataaat gcataagcta ctccacctgg
    12541 accagcatat acctttgacg acttccctgg acgctgagta ctactaactc tcgcataatg
    12601 tctctcacgg gcatgataat aacttgaata tgctccccaa ttacttggat catgatgacc
    12661 tccgtaattc cactctccac cttctacaga gatagcgagg accggaactg caaagctcat
    12721 cgtcaatagc accaataaaa aagctagttt tcttgaggtc ttaaacatta ctacctctcc
    12781 tttccgtacg ctttaatact aactgactta aaacaattat aacccttgtt catgaccaat
    12841 atcgtttcct atcttttcct cctttctcag tcactattaa ggatttataa aacaatttaa
    12901 atagtaaata taactactcc ctccggtgaa taatatcagg ttcccatccc tgtgttttta
    12961 ctttagctaa tgggaatagg gtcaataata tagttaccat caaaagaatt gatatatatt
    13021 tcttcataaa atcacccttt tttgtttttt attttaacat attaatgacg aatttgcaac
    13081 tatttttagt tattttacaa aaaacaatct ttatataatt attaaatatc ttttgataga
    13141 tactctttta aattgagata actattttgc ggaccaaaaa atttgaatag acattccgct
    13201 tctctataat aggagagaat ttcatctttt gatagaaatc cctctccaac atttgcgatt
    13261 aaacaaagcg tatctgccaa aagataacaa ctcttcttat ctcgcaagat ttcgattagg
    13321 tctgtcaatt cgtcaattgc ctgtgactgt ctctttcttt taaaaagata atgagcgtga
    13381 ttgtagcgaa tcttgatata cttatgaaaa cgatctgtat ctgttatatc caaatgacta
    13441 agcttttctg aaatctcctc atacaaactt tctagatcct catctcggga agtcaaggca
    13501 taaaaattag ccaaactact gtatacatcc agataaaaac tagatttctt actgattcga
    13561 tgactaagtt cttccatacg tacaattgca atatcctcct tatgataatg atgaaagtcc
    13621 actatacaag ccagataagt taaatattca ctgctttcct gcgaatgaac aacctctttc
    13681 tgcttctctg cctctacaag ataagccagt tgatcatagg aacgagtgaa caaagctttg
    13741 tccaccaatc gtttaaatac cgtaatatca ggcgctttat ctgatacact ttcatcaaaa
    13801 aaatagtcca tagatacctg taagcgttgt gataaggcaa ataatatatc cgatgtagga
    13861 gcaactccta atttttcaac tcgactaatc aaactctgct cacaaattcc ctctgcaagc
    13921 tccttttgag taaagccaag ttcctttctt ctgtttttaa aacgtttact aagaatcatt
    13981 ttcataacct ttctgtatat ttacatctta aataaaatct ctataagtca ttttatcaaa
    14041 attatctttt ctaacatact atagtggctt gaattaggaa aaggacggcc ttaagttata
    14101 gtgttaataa cccactccta aaataacttt agagttctca aattgacaaa cttagatatg
    14161 tcccgtttct aattcacttg actatatttc gacacaaaaa gcctattttt tgcgaataga
    14221 ctttctgcgt tcataaattt atttaagcaa atccaaaaac aggccatatc ctgcttcttc
    14281 catttcctct ctcggtatga aacgaagggc tgctgagttg atacagtaac gtaatccacc
    14341 cttgtcacgc ggtccgtcat caaagacatg acctaaatga gcatgaccag ctcgactgcg
    14401 gacttcaata cggttcatgc cgtgggacct atcttggaaa taagtcgcaa cttccttgct
    14461 gattggtttg gtaaaggagg gccagccgca gcctgagtcg aatttgtctg tcgagagaaa
    14521 gagcggctcg ccagtcgtaa tatcgacgta aatgccagct tggtcgctat tccaaaactc
    14581 attttcgaag ggcctttccg tcgcagcttc ttgagtgact tgatactgga ggtcggtcaa
    14641 gctgtctttt aagacttgtt ggtctggctt ttggtagtct gcaacatcaa tcagagggat
    14701 ttttgcctgc ccaagatcga tatggcagta cccttgggga tttttcttga gataatcctg
    14761 atggtagtct tcggcgggga tgtaatgtgc gagtggctcc acttcaacgg ctagggggcg
    14821 tccgccaaag agctgggact gctccgtcat gacctgctcg atggtcggta aatcagcctc
    14881 atctgtgtaa tagataccag tccgatactg gcgccccttg tcaggcccct gctgattgac
    14941 agagaaggga tcaatgacac ggaaatagta gaggaggatt ttccgcaaat tgatacgagt
    15001 ctcatcatag gctagataga gcgtctctgc gtggtctgtt tgtttgagga gttgataatt
    15061 ggtcgtctcc acctgtccat tggcatagcc aacgctggtg tcaagaatac catcgatctg
    15121 ggaaaagtag ccttccatcc cccagaaaca accgcctgct aggtagattt ctttcatgtg
    15181 aactccttct tatctgcaag cgtggtttcg tcacccttac agattttttt ctttcatttc
    15241 tgcttccatt ttgaggcgtt gaaccttcat agtcgcagta cgtggaatgt cgtcataagc
    15301 catcaagata ggctctttca atagtggtag gtctgcaacc atggtccacc aagcttccca
    15361 attcatctca gcatcttctg ccagggcgat aatcggctgc ggtgctccat tgacatcgcg
    15421 gataatcacg acttctgata aaaagtccaa tttatccagc aataaatctt ccaaggcaag
    15481 gttgctgtcg atgtgctcaa tgaggtcaac ctgacggtct tttagaagaa gcgtaccctc
    15541 tggagtcatg cagccgtagt caccactatc ccaccaagta ccgtacacat tatcttggaa
    15601 gcgggcatct tctttgtagt aagtcacagc acggcctttt gacaggaact ggatatgtcc
    15661 gttttctcca gctgggagag gattcccttg ggcatctgtg atgcgtgctt ctgtgtaacc
    15721 ttccagacca atccccatat ctcgtccgct aactgttcct agattttcca agcgatggta
    15781 acgtaaaatc attggaccac attcactttg accataaacc tgcataaaga ctggattttg
    15841 ctctttagaa gcctccaaga aggcgcgaag cgtaccaata ttgatagcat caaaggttga
    15901 atggtaataa cgaatgctgc taaagacctc tggtttttcc ttggccaatc ttgcccattg
    15961 aacaaagtta ttaggatggg tttccagagc gcttggacgg tgaagagcaa gtgcccgttc
    16021 aacatcatct tttcttgcag aagaaagcgg gtagaggggg aatccaagcc caatcgccga
    16081 agacactccg atattatagc gcgaatgtac aggggagata tggaaggcca gcaagcctct
    16141 ctccgtcatc ttgtcaaaaa tagtttgttg ccaagctact cgccagccca tggtctgtgc
    16201 tgtgtggcag attagttttg gaacacctgt cgttcctgaa gtatgggtca tgtattgaat
    16261 gacatcttct ggcaggagat tttcttcaaa agcaactgct tcttgcacta ggacttgggg
    16321 taaaaatatc tttgtgacca ggtcagttcg agtcattcct gctacacgtt cttccgtttc
    16381 ttggtcgtag acaatgaaag acttttccaa tcgctccgca aacacatcta aattcgaaga
    16441 tggcaaatgg taggaaatca taacaggaac agcacccaat gccgttaccg ctactgctaa
    16501 caaataggtg tcgaaagctg ggctcttata cagaaccacc ttgtccccat agccaatacc
    16561 tgccgctgca aattgagcag ctcttttttg aatggcctca aaaactgttt gataggtatt
    16621 ttccaatact aattcaggga aggatgcgta gggttggtca aaataaatgg caacatttgg
    16681 aaactcttga gctgctgttt taaactgacg atagagattt aatggtttgt aactaatagt
    16741 tgacatatct aacctttcta aaatgacaat gataaggtta ttataacatc ttgtgccaac
    16801 cttcacaatc tgatatcttt atatgactca cagttgtgaa aatgaaagca agtagtcaat
    16861 caccacttgc tttcattttc aattaaccaa tctctgccaa gatagctttg atttgatttt
    16921 tcgtgtggac accagcaact tgtttcacaa cctgtccatc ttttttgaac aaaagagttg
    16981 ggatagacat gattccaaac tgacgagctg tatttggatt ttcatcaaca tccatcttgt
    17041 aaatacgcaa ttcatcttcg tccacttcat ctgccaattg ctccaaaatt ggtgcttgca
    17101 tacgacaagg accacaccat ggtgcccaaa agtcaacaag gactacacct tcttgtgttt
    17161 caacttcaaa gtttgcatct gtaataactt gaaccatgat tttctccatt tttgtttttc
    17221 tactatcatt ctacaccaaa aaaaaacaga aagcgaattt ttgctacgga agagaaatga
    17281 gatagacatg c
//
LOCUS       DATUCG010000043        17003 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.044, whole genome shotgun sequence.
ACCESSION   DATUCG010000043 DATUCG010000000
VERSION     DATUCG010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17003
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.044"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            362..625
                     /locus_tag="VCA20_001106"
     CDS             362..625
                     /locus_tag="VCA20_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEP1834945.1"
                     /translation="MASKDFHIVAETGIHARPATLLVQTASKFASDITLNYKEKSVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAISETMEKEGLA"
     gene            630..2363
                     /gene="ptsP"
                     /locus_tag="VCA20_001107"
     CDS             630..2363
                     /gene="ptsP"
                     /locus_tag="VCA20_001107"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEP1834946.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETVTVEDTNAEEARL
                     DVALEASQNELSVIRENAVASLSEEAAAVFDAHLMVLADPEMIGQIKETIRAKKTNAE
                     TGLKEVTDMFIAIFEGMEDNPYMQERAADIRDVAKRVLAHLLGVRLPNPATIDEESIV
                     IAHDLTPSDTAQLNKQFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNNITEIVKDGD
                     VLAVNGITGDVVINPSEEVIAEFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNDNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDLPHEMNPFLGFRALRISISETGNQMFRTQLRALLRSSVHGKLRI
                     MFPMVALLTEFRAAKAILDEEKANLLAEGVAVADDIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYAHRALTECATAEEV
                     LELQKEYVDFD"
     gene            2573..3997
                     /locus_tag="VCA20_001108"
     CDS             2573..3997
                     /locus_tag="VCA20_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HEP1834947.1"
                     /translation="MKAYNNYINGSWVGSEKSIAIHSPINQEKLGTVPAMSKEEVDKA
                     MDAARKALPAWRALSAFERAQYLHKAANILEERKDAIGEILAKEVSKGIKSSIGEVDR
                     SAHLIRFAAEEGLRVYGSSLEGGTYEPTAKSKVSLVRREPVGIVLAIAPYNYPVNLSA
                     SKIAPALIGGNVVMFKPPTQGSISGLLLAEAFADAGIPAGVFNTITGRGSEIGDYIIE
                     HPEVNFINFTGSTPIGQKVGKLAGIRPIMLELGGKDAAVVMEDADLDHTAKEIVSGAY
                     SYSGQRCTAIKRVLVMDSVADELAAKLKAEVEKLTVGDPFENKDITPVIDEQSAEFIE
                     GLVRDAQAKGATELTPYKREKNLIWPTLLDHVTPDMEIAWEEPFGPVLPIIRVSSLDE
                     MLELCNQSEYGLQSSIFTNNYPFAFDFASKLEVGTVHINAKTQRGPDNFPFLGVKGSG
                     AGVQGIRYSIEAMTKVKSVVFDVQ"
     gene            complement(4257..5744)
                     /locus_tag="VCA20_001109"
     CDS             complement(4257..5744)
                     /locus_tag="VCA20_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEP1834948.1"
                     /translation="MKKVAFDSTKYLNLQRDHILERIAQFEGKLYMEFGGKMLEDFHA
                     ARVLPGYEPDNKIKLLQELKEQVEIVIAINASNIEHSKARGDLGISYDQEVFRLIDTF
                     NDIDIYVGSVVITQYRNQPAADAFRKQLEKHGIKSYLHYPIKGYPSDIDHIISPEGMG
                     KNDYIETSRNLVVVTAPGPGSGKLATCISQLYHDQLHGVTSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDLNMIDPFHLQTYGKTAVNYNRDIEVFPVLNRTFERILNKSPYA
                     SPTDMGVNMVGYSIVDEEAAIEASKQEIIRRYYQTLVDFKAERVSEQAVKKIELLMNE
                     VGVTPADRKVVIAAREKAELTTSPALAIQLPNGEMVTGKTSDLLKPTATVLLNAIKQI
                     ANIDDETLLIEPNYIRPIQKLKADYLDKTNTRLDASEILNALAITAQDSPVAARAMKE
                     LGQLNGSEAHSTVILSDEDKSVLRKLGINLTFDPIYQHNKFYQAK"
     gene            6018..7286
                     /locus_tag="VCA20_001110"
     CDS             6018..7286
                     /locus_tag="VCA20_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEP1834949.1"
                     /translation="MKKLIHNKLFLSSFVADVISNFGDTLYYLALMNYVLLLPDTKFA
                     LAMITASETLPILAGLFIGIWADKTKNKLDTILATLVARVGLYALVGLLMGFTPALWI
                     VAVVCLVNFLSDLAGQYENGLYMPLSLRVLEAEDRETAMAFKNTVGGILMIVFRSSGA
                     ILIGYMSYQNLAFFNAGTFLVSLAIMVGLRPAFAKLLKENPIQEVEQTETEAGLVKGI
                     GQSLKESYQAIQNIPVLKASILTISGINTVVSAVSPLVILSMKEFSDFVIVNSGITLA
                     LVFNLFSVGYILGSSLGMAFFKHISLSKLLKLCTGLSITLFVGFYLYNIYIVMVTILV
                     TTMTTGIINPKLYALIMNELPEDKLATINAGMGTIFNIGILAGQALVALMVTILSVTS
                     ISLIFLLLSVGLVSYTILVGRKPKNEVAQV"
     gene            7298..8338
                     /locus_tag="VCA20_001111"
     CDS             7298..8338
                     /locus_tag="VCA20_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HEP1834950.1"
                     /translation="MKLDYRDYRSEIVEKFFIPLIVTEREEPFEADFSQKEKDILKDA
                     LDIRDEIEEKLADFRQEVNQVFVWGHIFNILHSLYFYILNDGQDPKTVEEACQLILKL
                     SQEEVEDAMRTMLASENDGHREKTLSLMELLEKTDKKPADKWYWSLAIRNPLETVQRS
                     VHLLDKMLPIYQPYFEQARVEREAFARDFDIEQLYRESKQLAMTSLDSLGVETAQFFV
                     LSPWCFWFAYYGNEEFDTMKVALLASCRIDQILLSNEELDLDDLTNALKVISDSTRYQ
                     VLVELTKPHAKSKDIAERLNITGAAVSFHTQKLINGDLLLFNTKNSDVKYSVNRDLLQ
                     QVIDKLKENFDL"
     gene            complement(8456..9463)
                     /locus_tag="VCA20_001112"
                     /pseudo
     CDS             complement(8456..9463)
                     /locus_tag="VCA20_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299099.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9652..10338
                     /locus_tag="VCA20_001113"
     CDS             9652..10338
                     /locus_tag="VCA20_001113"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEP1834951.1"
                     /translation="MKDLHAKLLADFGIDFSDLNLLETAFTHTSYANEHRLLKISHNE
                     RLEFLGDAVLQLMISKYLYQKYPDRPEGEMSKLRSTFVREESLAGFSRACGFDRFLRL
                     GKGEEKSGGRNRDTILGDAFEAFLGALLLDKGEKTVEEFIHKVMIPRLEKGNFERVTD
                     YKTALQEILQVNGEIAISYQVIAESGPAHDKTFEVEVSADQRVIGRGRGRSKKLAEQA
                     AAKNAVEARG"
     gene            10341..13874
                     /gene="smc"
                     /locus_tag="VCA20_001114"
     CDS             10341..13874
                     /gene="smc"
                     /locus_tag="VCA20_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEP1834952.1"
                     /translation="MYLKSIEMQGFKSFADKTKVVFDRGVTAVVGPNGSGKSNITESL
                     RWALGESSAKSLRGGKMPDVIFAGTESRKALNYASVVVTLDNSTGFIANKQKEIKVER
                     HIYRSGDSEYLIDGQKVRLRDIHDLFMDTGLGRDSFSIISQGRVEAIFNSKPEERRAI
                     FEEAAGVLKYKTRKKETESKLVQAQGNLDRLDDIIYELDNQVKPLEKQAQTAKKFLEL
                     DGQRKELYLDVLVAQLTKGKETLTGKESELETVKSELASYYKQRFELEQENQNLKEKR
                     HRLSEQLEREQAVLLDLTKLISDLERKIEVHKLESSQNESSRQEAQARLENLLTRREQ
                     LAEQIEQKQETLAQLDSSLSSLKDDIAAVDKEISYFSEDPDQVLDHLREQYVALMQEE
                     AEASNRLTKIQQDIANQISLSESKSEDLARLQTEKQTAQEVLDKSRKSLEEADHVLRQ
                     LLESYQTQKTELDQVQTAYQAEQGRLFDLLDQLKGKQARQSSLEAILKNHSNFYAGVK
                     AVLQAAPSLGGIIGAVSEQLTFDTRYQTALEIALGGASQNVIVEDEATAKRAIAFLKE
                     KRQGRATFLPLTTIKPRQLSGQQVSLLESSEGFLGLASDLVTYQPNLDAIFQNLLGTI
                     AIFDTIDHANQAARATKFQVRMVTMDGAEIRPGGAFAGGSNRNNSTTFIKPELDALLG
                     EIAELSSQLQEQESLVAAKKTSLDQTREALETIKAEGEEARLNQQSARIHQEQAENRL
                     AQLSAQYDLQMSQVSPNILTELEEAAAKEEVNVQALNEKKLALDQQINQVRDNRDSIQ
                     ESLQKLQTQKGQLTLEQAELTSQLRFEQTDLRRLQEEKVVADKEISLLEDMIDQKLEA
                     LEDTSIEILEEQLQAASDKQNQTNQILIRLKFELEDIDGQFEDLEERLQQARTKNDDL
                     IRKQAKLEADCEQAGDKLRTLLGNLTEHFKLSFEAAQSQAKEVENLAVAEQSLKDLER
                     AIRALGPVNLDAIEQYDEVNNRLTFLNEQRTDILSARDLLLNTIHEMDDEVKERFKVT
                     FEAIRESFKQTFKQMFGGGSADLILTEGDLLTAGVEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFSIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKESQFIVVTHRKGT
                     MAAADAMYGVTMQESGVSKIVSVKLKDVEKL"
     gene            13871..14680
                     /locus_tag="VCA20_001115"
     CDS             13871..14680
                     /locus_tag="VCA20_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEP1834953.1"
                     /translation="MINKIYASDMDGTFLREDHSFDKERFRRILNQFKEKGYLFVAAS
                     GRSMQSLKLVFEDFVDEIGFVAENGSIVEYQGQTIFMDDPISPEIYLPIIAGIDAGPF
                     GSSQSMVLSGLENFYLLKNAEPQFLEAMTNYYAHFRLVDTFEEVREEIIKINAKFSPE
                     EMDDARRWLNDTFEGVTAMTTGFDNIDIIPNGSNKSVGLSHLCHHFGITGQDVVAFGD
                     NQNDLDMFNFAGLALATENAREEVKARADLMIGHCNDGAVLAYLEEEVNGN"
     gene            14670..15482
                     /locus_tag="VCA20_001116"
     CDS             14670..15482
                     /locus_tag="VCA20_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEP1834954.1"
                     /translation="MAIKLIALDLDGTLLTSDKRISEANKKALQVARERGVYVVLTTG
                     RPLQAIGTFLEELDLLGEKQYSIAFNGGLVQENTGRVLDKVGFTIDDVRTIRRVTNDL
                     DLPLDVLYGGDIYSLPSAHESLYLTCNPLLNKIVASDEEMPEDFVYNKAVSAVAADFL
                     DGQIPKIPAELHERFEIFKSRDILLEWSPKGVHKANGLAKLIGHLGIDQSEVMACGDE
                     GNDLSMIEWAGLGVAMANATDEIKSAANLVLPKNNDEDGIAWVIEHYVLNED"
     gene            15484..16758
                     /gene="ftsY"
                     /locus_tag="VCA20_001117"
     CDS             15484..16758
                     /gene="ftsY"
                     /locus_tag="VCA20_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEP1834955.1"
                     /translation="MGLFDRLFGQKKQEEQVALVEDIETEQSASSEDTVTTEVESATQ
                     EMEEFQSEVAEPELTAEQEAQKQRTLDMMAQYYAAKEAAAARVKAAQEQGFDPAIQTK
                     VKEEPVEEVAPVEQESEQDKYQRTLKKTRTGFGARLNEFFANFRSVDEEFFEELEEML
                     ILSDVGVQVASTLTEELRYEATLQNAKKTDELRRVIIEKLVDIYEKDGQFSEQINFQD
                     DLTVMLFVGVNGVGKTTSIGKLAYKYKQAGKKVMLVAADTFRAGAVAQLVEWGRRVDV
                     PVVTGPEKSDPASVVFDGVKRAVAEGVDILMIDTAGRLQNKENLMAELEKIGRIIKRT
                     LPDAPHETLLALDASTGQNALSQAKEFAKITPLTGLVLTKLDGTAKGGVVLAIRQELD
                     IPVKLIGFGEKIDDIGEFKSEEFMRGLLEGLV"
     gene            complement(16782..>17003)
                     /locus_tag="VCA20_001118"
     CDS             complement(16782..>17003)
                     /locus_tag="VCA20_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEP1834956.1"
                     /translation="IINVLFLFHLTMLEEVGCLRITAVHDSENPPSGRVMEKVGMTYE
                     GTLRQAARNNRGIVDIAIYSLLHTDGKSR"
BASE COUNT         4961 a         3111 c         4118 g         4813 t
ORIGIN      
        1 acgaccagtg gactttctac ctcgtaaatc ttgggagggt aaagtagggt cttctcggta
       61 ttttcttgtc caagttaaaa cttggttgtc tgattttaac ccatattttt tgacaataga
      121 aggcatccct ccgctctttc cgctgaggta gtcttcaact acctgtagtt tgaactcggg
      181 tggatatttg gtgaacttgc ttcctttttt agccatgaaa aaagaaactc tctttttatg
      241 acggaaacat gaaagaaagg taaaaaaatc ttgaaaatga tttcaaaaag tggtataatg
      301 tcatttgtaa gggttatcac aaataactcg caattttgta aaaataaagg agaatccaaa
      361 tatggcttca aaagacttcc acattgtggc agaaacaggt atccacgcac gtccagcaac
      421 tttgcttgtg caaacagcta gtaaatttgc ttcagacatc actttgaact acaaagaaaa
      481 gtctgttaac cttaaatcta tcatgggtgt tatgagtctt ggtgttggtc aaggcgctga
      541 cgtaacaatc tctgctgaag gtgctgatgc tgacgacgca atcgcagcta tcagtgaaac
      601 aatggaaaaa gaaggattgg cataagaata tgacagaaat gcttaaagga atcgcagcat
      661 cagatggtgt tgctgttgct aaggcatatc tactcgttca gccagatttg tccttcgaaa
      721 ctgttacagt tgaagataca aatgcagagg aagctcgttt ggatgttgct ctagaagcat
      781 ctcaaaacga gctttctgtt attcgtgaga acgcagtagc tagcctaagt gaagaggccg
      841 cagccgtatt tgatgcacac ttgatggttc ttgctgaccc agaaatgatt ggtcagatta
      901 aggaaacaat tcgtgcgaag aaaacaaacg ctgagactgg tttgaaagaa gttacggata
      961 tgtttatcgc tatctttgaa ggtatggaag ataacccata catgcaagag cgtgccgcag
     1021 atattcgtga cgtagcaaaa cgtgtattgg cacacttgct tggtgttcgc ttgccaaacc
     1081 cagcaactat cgacgaagag tcaatcgtta tcgcacatga cttgacacct tcagatacag
     1141 cgcaattaaa caaacagttt gtaaaagctt tcgtaacgaa cattggtggt cgtacaagtc
     1201 actcagctat catggctcgt acacttgaaa ttgctgctgt tttggggaca aataacatta
     1261 ctgaaatcgt taaagacggt gatgtattgg ctgttaacgg tatcacaggt gatgtagtga
     1321 ttaacccttc tgaagaagtg attgcagaat ttaaagcagc tggtgaagcc tatgctaagc
     1381 aaaaagctga atgggctctt ttgaaagatg ctcaaactgt tacagctgac ggcaaacact
     1441 ttgaattggc agcaaatatc ggtacaccaa aagacgttga aggtgtaaat gataacggtg
     1501 cagaagctgt tggtctttac cgtacagaat tcttgtacat ggactcacaa gacttcccaa
     1561 cagaagacga gcaatacgaa gcttacaagg ctgttcttga aggtatgaac ggcaagcctg
     1621 ttgtggttcg tacaatggat attggtggtg ataaggaact tccttacttc gaccttccgc
     1681 acgaaatgaa cccattccta ggtttccgcg cccttcgtat ctctatttct gagactggta
     1741 accaaatgtt ccgtactcag ttgcgtgccc ttcttcgttc atcagtacat ggtaaacttc
     1801 gtatcatgtt cccaatggtt gccttgttga ctgaattccg tgcagcaaaa gcaatccttg
     1861 atgaagaaaa agcaaactta ttggctgaag gtgtagcagt tgcggatgat atccaagttg
     1921 gtatcatgat tgaaatccct gcagcagcaa tgcttgcaga ccaatttgct aaagaagttg
     1981 acttcttctc aatcggtacg aatgacctta tccagtacac aatggctgca gaccgtatga
     2041 acgaacaagt ttcatacctc taccaaccat ataacccatc aatccttcgc ttgatcaaca
     2101 atgttatcaa ggctgcacac gcagaaggta aatgggctgg tatgtgcggt gagatggctg
     2161 gtgatcaaca agctgttcca cttcttgtag gtatgggctt ggatgagttc tctatgagtg
     2221 caacatctgt acttcgtaca cgtagcctta tgaagaaact tgatacagct aagatggaag
     2281 aatatgctca ccgtgccttg acggaatgtg caacggcaga agaagttctt gaacttcaaa
     2341 aagagtatgt tgatttcgat taataagaaa tttaaagttt ggattagata aactacttca
     2401 aacttttttt gtatgctcca gcattgagta ctatatattt tttgagatat acggcttaaa
     2461 atactttgaa gattgaaatt aatttgaaag aaaatttgaa attttcaaaa aattattgct
     2521 aaaccaggtt caatatgtta caataatgac attatactca aaggaggaaa aaatgaaggc
     2581 gtataataat tatattaacg gttcttgggt tggatcagaa aaatcaatcg cgattcactc
     2641 accgattaat caagaaaaac ttggtacagt acctgctatg tcaaaagaag aagttgataa
     2701 ggcgatggat gctgctagaa aggcattgcc cgcttggcgt gccctttcag catttgaacg
     2761 tgctcaatat ttgcataagg cagcaaatat tttggaagag cgtaaagatg caatcggtga
     2821 aattttagct aaagaagttt cgaaagggat aaagtcttcc atcggagaag tggatcgttc
     2881 tgcccacctc attcgttttg ctgctgaaga aggtttgcgt gtttatggtt catcattaga
     2941 gggtggaacc tacgagccaa ctgctaagtc aaaggtttct ttggttcgtc gtgaaccggt
     3001 aggtatcgta ttggcgattg caccgtataa ctaccctgtt aacttatcgg cttcaaaaat
     3061 tgcacctgct ttaattggtg gcaacgtagt tatgtttaaa ccacctacac agggttcaat
     3121 ttcaggtcta ttgttggcgg aagcttttgc agatgctggt atcccagcgg gtgtctttaa
     3181 taccattact ggtcgcggtt cagaaattgg tgactacatt attgaacatc cagaagtaaa
     3241 cttcattaac tttacgggtt caacgccaat tggacaaaaa gttggtaaat tagcaggcat
     3301 tcgtccaatt atgcttgaat tgggtggtaa ggatgcagca gttgttatgg aagatgcgga
     3361 tttggaccac actgcaaaag aaattgtaag tggtgcctac agctattcag gtcaacgttg
     3421 tacggctatt aagcgtgttc ttgttatgga ttctgtagct gatgaattgg ctgcgaagtt
     3481 gaaagcagaa gttgagaaat tgactgtcgg tgatccattt gagaacaagg acatcacgcc
     3541 agttattgac gaacaatcag cagaatttat tgaaggtctg gttagagatg ctcaggcaaa
     3601 aggtgcaact gaattgacac catacaaacg tgagaaaaac ttgatttggc caacccttct
     3661 tgaccatgtt acaccagata tggaaattgc ttgggaagaa ccatttggac cagttcttcc
     3721 aattattcgt gtttcatcat tggatgaaat gttggaattg tgtaaccaat ctgagtatgg
     3781 tcttcaatct tctatcttta caaacaacta tccatttgct tttgattttg cttctaaatt
     3841 agaagttggt acggttcata tcaatgccaa gacccaacgt ggtccagata acttcccgtt
     3901 cttgggtgtc aagggttcag gagcaggtgt tcaaggtatc cgttacagta tcgaagcgat
     3961 gactaaagtg aagtcagtgg tatttgacgt tcaataaaat aataagggag tgggacaaaa
     4021 atcggtaacc cgcagggttc gatgagtcgt tagcccaccc ccgcacagtt gagtagggct
     4081 gtaaaagctg ataaaatcag ctaattagag cccactcaac cactgcgtca agtttaacta
     4141 ttgaaaaaaa ctaaggaggc caggactttt gtcccagcct ctttttctta catacaggta
     4201 accattcatt cacgaaaaaa gttctaacct caaaatgaga ttagaacaga atttggttat
     4261 ttagcttgat aaaacttatt gtgttggtag atagggtcaa aggtaagatt gatgcctaat
     4321 ttacgaagga cactcttgtc ttcatcggat aggataacag tcgaatgagc ttcgctacca
     4381 tttaattggc ctaattcttt catggcacgt gcagcgacag ggctatcttg tgcagtgatg
     4441 gcaagtgcgt taagaatctc acttgcatcg agtcgtgtat ttgtcttatc cagataatct
     4501 gccttcaatt tttgaattgg acggatgtag tttggttcga tgagaagtgt ttcatcatcg
     4561 atgttagcaa tttgcttgat ggcattgaga agaacagttg cagttggttt gaggaggtcg
     4621 gatgtcttac ctgttaccat ctctccattt ggcaactgaa tagctagggc agggctggtt
     4681 gtcaactctg ctttttcgcg cgcagcgata acaaccttac gatcggctgg tgtcacaccg
     4741 acttcattca tgaggagttc aatcttttta actgcttgct cgctcacccg ctcagccttg
     4801 aaatcaacca aggtttggta gtagcgacgg atgatttctt gcttggatgc ttcgatcgca
     4861 gcctcttcgt ccacgatgga atagcccacc atgttaacgc ccatgtctgt cggtgaggcg
     4921 tatggtgatt tgttcaaaat acgttcaaag gtacggttga ggacagggaa gacctcgatg
     4981 tcacggttgt agttgaccgc agttttgccg taagtttgca ggtggaaggg gtcaatcatg
     5041 ttgaggtcgt ccaggtcggc agttgctgct tcataggcca agttaacggg atggtggagt
     5101 ggtaagttcc acactgggaa ggtttcaaac ttggcatagc ctgatgttac gccatgcagt
     5161 tggtcgtggt aaagttggga gatacaagtc gccaatttac ctgaaccagg tccaggtgcg
     5221 gtgacaacga cgaggttgcg actagtttca atgtagtcat ttttgcccat accttctggt
     5281 gaaatgatgt ggtcgatatc agaaggatag cccttgattg ggtagtggag ataggacttg
     5341 ataccatgtt tttccaactg tttgcggaag gcatctgcgg ctggttgatt gcggtactgg
     5401 gtaatgacca cagaaccaac ataaatatca atatcattaa atgtatcaat caagcggaag
     5461 acttcttggt cgtaagaaat gcctaggtcg ccgcgtgcct tagaatgttc gatattgctg
     5521 gcgttgatgg cgatgacaat ttctacttgt tcttttaact cttggaggag tttgattttg
     5581 ttgtctggtt catagccagg taaaacacgg gctgcgtgga aatcttccaa catttttcca
     5641 ccaaattcca tatagagctt gccttcaaat tgggcaatac gctctaaaat gtgatcacgt
     5701 tgcaaattca agtatttagt tgaatcaaat gcgacttttt tcattataaa tatgtcctcc
     5761 aaatgtgtat taatctctgg gaaaacaaaa aatagctccc taaaactagg gagccttttc
     5821 tccgacctct caaaagaggt gcccgaacaa tactataaca aaccgtgcta atcttgtcaa
     5881 gaaaaagtca gatagaagaa aaattaaaca agtgtttaaa aaatatttaa tttttataaa
     5941 attaaacatt gactttatta aaataaagtg taaaattata ttgaacaaat atttaattta
     6001 tgtttaagga gatgtttatg aaaaagttga ttcacaataa attatttcta tctagctttg
     6061 tagcggatgt gatttccaac tttggagata cgctttacta cctagctttg atgaactatg
     6121 tcttgttgct gccagatacc aagtttgcct tggctatgat taccgcatct gaaaccttgc
     6181 cgattttggc tggtctcttt atcgggattt gggcggacaa gactaaaaat aaactggata
     6241 ccattctggc taccttggtc gcacgtgtcg gtctgtatgc tctcgttggt ttgctaatgg
     6301 gatttacacc agccctctgg attgtagcag ttgtttgtct ggtcaatttc ctatctgact
     6361 tggctggtca gtatgaaaac gggctttata tgccacttag cctgcgggtg ttggaggcag
     6421 aagaccgtga aactgctatg gcctttaaga acaccgtggg cggcatcttg atgattgtct
     6481 ttcgatccag cggtgccatt ctaatcggtt atatgtctta ccagaatctt gcctttttca
     6541 acgctgggac cttcttggta agccttgcga ttatggtagg tttgcgtcca gcttttgcca
     6601 agttgttgaa agaaaatcct atccaagagg ttgaacaaac cgaaacagaa gcaggacttg
     6661 tcaagggaat tggtcagagc ctcaaagaat cttatcaagc cattcaaaac atccctgtat
     6721 taaaggcttc tattctgacc atttcgggta tcaatacggt tgttagtgcc gtatctccct
     6781 tggttatcct gtctatgaag gaattttcag acttcgtgat tgtcaattct ggcataactc
     6841 ttgccttggt attcaatcta ttttctgttg gttacatcct tggctctagt ctaggtatgg
     6901 ctttcttcaa acatatcagc ttgtccaagc tactcaagct ctgtacaggt ttgtcgatca
     6961 cgctatttgt tggcttctac ctttataata tttatattgt aatggtgact atcttagtca
     7021 ctaccatgac gactggtatt atcaatccca aattgtatgc cctgataatg aatgaactac
     7081 ccgaagataa attggcgact atcaatgcag gaatggggac tatattcaat attggaatcc
     7141 ttgcaggtca agccttggtc gcccttatgg taacaatctt atctgttaca agtatttctc
     7201 tcatcttcct cttgctttct gtgggactag taagctatac tattcttgtg ggtcgtaaac
     7261 ctaaaaatga agtagctcaa gtttagaaag tatgcgtatg aaattagatt accgagatta
     7321 tagaagcgaa attgtcgaaa aattctttat tcctttaatt gttacggaac gcgaggagcc
     7381 gtttgaggcg gatttttcac agaaagaaaa agacattctc aaagatgcct tggacattcg
     7441 tgatgagatt gaagaaaagt tggcagattt tcgtcaggaa gtcaatcaag tctttgtctg
     7501 gggacatatt tttaacattt tgcatagtct ttatttctat atcttgaatg atgggcagga
     7561 tccaaagaca gttgaggaag cttgtcaatt gattttaaag ctgtctcaag aagaagtaga
     7621 agatgccatg cgaaccatgt tggcatctga aaatgacgga catcgtgaga agaccctcag
     7681 cctcatggaa cttttggaaa aaacggataa aaagccggca gataagtggt actggtcatt
     7741 ggctatccgc aatcccttgg aaacggttca gcggtctgtc catctcttgg ataagatgct
     7801 tccaatctac cagccctatt ttgagcaagc aagagttgag cgggaagcct ttgctaggga
     7861 ttttgacatt gaacaactct acagagaatc caagcagtta gccatgacca gcttggatag
     7921 cttgggtgta gaaactgccc aattcttcgt tctcagtcca tggtgtttct ggtttgccta
     7981 ttacggcaat gaagaatttg acactatgaa ggttgctctt ttggcttcct gtcggatcga
     8041 ccagattttg ctgtccaatg aggaactgga tttagatgac ttgacaaatg cccttaaggt
     8101 tattagcgat agtacgcgtt atcaggtctt ggtggagcta accaagcctc atgccaagag
     8161 caaggacata gcagagcgac tgaatatcac aggggcagcc gtatccttcc acactcaaaa
     8221 gctgattaac ggtgacttac ttctcttcaa tacaaaaaat agcgatgtca aatacagtgt
     8281 caatcgcgac ctgctccaac aggtgattga taagttgaaa gagaattttg atttgtaaaa
     8341 aagccttgta agttaagtct tacaaggttc ttttgtgctt atactcttcg aaaatcaaaa
     8401 tcagacgttg ttgacttgat ttgacgaact tcagttctat ctgcatcagc gtcgcctagt
     8461 ctgtttttga ttttctttga gtattaatga ttaaccagct gagcagttga caagtgatga
     8521 tggtgataag gaaaaccagc cagccttgct gtattttggt cagggaacta gtttctagga
     8581 agaaaaagct gatttcgact gggataacat ggataaaata ataatagctg gcgtattctt
     8641 ttagaaattg gaattttttc tggcggaaga ggtctgtggt taagctccag gcgacaagat
     8701 aggctgtgaa tggaatcagg ctaaatagaa agttcttgtc gtaccctgat tgagatagat
     8761 gacagtggtt tcaaaggcta aaagggctat gcagacaagt agtttgtttt ttgtcggtat
     8821 tggaaaatgg ggtgattgag tttgtcggct agataaaaag cctgtcagga caaaaatcgg
     8881 tgcgtagaag aggccgttgc ggctggtgaa aaagaaggtt ttgtagctgt caaatgcggt
     8941 caggaaaatc gattctgcca ggtaggagga gtaggtttcg actgagccta gaagatagag
     9001 aaggtaggct agacaagctg tgatgcgcca gccccatttc tccaaggaaa aatggacgag
     9061 gataagacct agaaagaaag cgggcatgta ccagagttgg tagctgttgc cggtgtaggt
     9121 cagtccgact aggagtgcga ggggatagta ggggaagggg atttcttgga gtgttaggta
     9181 gatgaggtag aagggcaggt agagtgcggc ccaggtcaag tattgtttgc tgtagcggat
     9241 gaggtattgt ttggagaggc tgggggtagt tgcgtcggag gaagaagctg gctgtgacca
     9301 tgaagtaggg aacgaccatg cggctaaaga ttgatttttg gatgaagtgg agctggtctg
     9361 cttcaaataa cctgcggcaa tggagggtga taacgagaat ggctccgatg aactggacga
     9421 gggtgagcag tgatgtattg agtggttttg gtttgttttg catgttaact cccttctttt
     9481 ttcttccatt ttatcgaaat ctgatgaata tgtaaatgtt tgcaaaaaga tgtggagagg
     9541 tgctggctga aacttagttg acaagagaag tccagtggaa aatatggtat aatgttagga
     9601 cggagtaaat gtaaaccatc tatttcgttt aagttagaaa ggagcatata gatgaaagat
     9661 ttacatgcaa aattattggc tgattttggg attgattttt cggatttgaa tttattagaa
     9721 acggctttta cccatacttc ttatgccaat gagcatcgcc ttctaaaaat ttcacataat
     9781 gagcgcttgg aatttttagg agacgctgtt ctccaattaa tgatttccaa atatctctat
     9841 caaaaatatc ctgatagacc cgaaggtgag atgtccaagt tgcgttcgac ctttgtacgt
     9901 gaggagtctt tagctggttt ttcacgtgct tgtggttttg atcgttttct tcgtcttggt
     9961 aagggggaag agaagtctgg aggacgaaat cgtgatacta ttttaggaga tgctttcgag
    10021 gcgttcttag gagctctgct attggacaaa ggtgaaaaga ccgtggagga atttatccat
    10081 aaggtcatga ttcctcgtct agaaaaaggg aattttgagc gcgtgaccga ctataagact
    10141 gctttgcagg aaattttgca ggtcaatggt gagattgcta tttcgtatca agtaatagct
    10201 gagtctggac ctgcccatga taagaccttt gaagtggagg tatctgctga tcagcgtgtg
    10261 attggtcgtg gtcgtggtcg ctctaagaaa ttagctgaac aagccgccgc taaaaatgct
    10321 gtggaggcta gaggataggt atgtatctaa aatcaattga aatgcagggc tttaagtcct
    10381 ttgcggataa gactaaggta gtctttgatc gtggagtgac tgctgttgta ggaccgaatg
    10441 gttctggcaa gtccaatatt acagaaagtc tacgttgggc cttgggagag tcgtctgcta
    10501 aaagcctacg tggaggcaag atgccagacg tcatctttgc gggaacagaa agccgtaagg
    10561 ctcttaacta tgcctcggtt gtcgtgacct tggataatag cacaggtttt atcgccaata
    10621 aacagaaaga aatcaaggta gaacgccaca tttatcgatc tggtgatagt gagtacttga
    10681 ttgatgggca aaaggttcgt ctacgggata ttcatgatct tttcatggat acgggcttgg
    10741 gacgtgattc tttctctatc atttcacaag ggcgggttga agcaattttc aattccaagc
    10801 ctgaggaacg ccgtgctatt tttgaagagg cggcaggggt cttaaaatac aagacgcgta
    10861 aaaaagaaac agaaagtaag ttggtacagg cccagggcaa cttggaccgc ttggatgata
    10921 ttatctatga actggataat caggttaaac cattggagaa acaggcacaa actgctaaga
    10981 aatttctaga attagatggt caacgtaagg aattatactt agatgttttg gtggctcagt
    11041 tgacaaaggg caaggaaact ttgactggca aggaaagcga acttgaaaca gtcaagtcag
    11101 aattagcaag ctactacaaa cagcgttttg agctggagca agaaaatcag aacttgaaag
    11161 agaagcgcca ccgtctgtca gaacaattgg aacgtgaaca ggcagttctg ttggatttga
    11221 ccaagttaat cagtgatttg gagcgaaaaa ttgaggtcca taaactggaa tctagtcaaa
    11281 acgaatcaag tcgtcaagaa gcgcaggcgc gcttggaaaa cttgttgact agacgagagc
    11341 agttggcaga acagattgag caaaagcaag aaacgcttgc gcaattagat agttccttgt
    11401 ctagcttgaa agacgatatt gctgcggtag acaaagaaat ttcctacttt tcagaagatc
    11461 ctgatcaggt tttagatcat ctgcgtgaac agtatgttgc tctgatgcag gaagaggcag
    11521 aagcttcaaa ccgtctgacc aagattcagc aggatattgc caatcaaatc agtctttctg
    11581 agagtaaatc tgaagacttg gcacgcttgc agacggaaaa acaaacagct caagaagtac
    11641 ttgataagag tcggaaaagt ttagaagaag ctgatcacgt tttacgccag ttgcttgaga
    11701 gctatcagac ccaaaaaaca gaattggatc aagtccagac tgcttatcag gctgagcaag
    11761 gtcgcttgtt tgatttgctg gatcagttga aaggcaagca ggctcgccag tctagtttag
    11821 aagctatttt aaaaaatcat tccaatttct acgcaggggt taaggctgtc ttacaggcgg
    11881 cgccaagctt aggaggaatt attggagcag tcagtgaaca attgaccttt gatactcgtt
    11941 accaaacggc tttggaaatt gctcttggcg gtgctagtca aaacgtcatc gttgaagatg
    12001 aagcgactgc taaacgagct atcgcatttt tgaaagaaaa acggcaagga cgtgcaacct
    12061 tcttacctct aacaactatc aagccacgtc agctttctgg acagcaagtc tctttactag
    12121 aatcttcaga aggattcttg gggcttgcaa gtgatttggt gacttatcag ccaaatttag
    12181 atgcgatttt ccaaaatttg ctaggcacta tcgcgatttt tgataccatt gaccatgcca
    12241 atcaggcagc gcgggcaacc aagtttcaag tgcgtatggt gaccatggat ggagcggaaa
    12301 tccgtcctgg tggagccttt gctggtggta gcaatcgaaa taatagcacg acctttatca
    12361 aaccagaact ggatgctctt ttaggagaaa tagcagagct gtctagccag ttgcaggagc
    12421 aagaaagctt ggttgcagct aagaaaacta gcttagacca gaccagagaa gccttggaaa
    12481 cgattaaggc ggagggggaa gaagctagat tgaaccagca gagcgcaagg attcatcagg
    12541 aacaggcaga aaatagactg gctcagctat cagctcagta tgacctgcaa atgagtcagg
    12601 ttagtccgaa cattttgact gagctagaag aggctgctgc taaggaagaa gtaaatgtcc
    12661 aagccttgaa tgagaaaaaa ctagccttgg atcaacaaat caatcaagtc cgtgataatc
    12721 gcgacagtat tcaagaaagc ttgcagaaac tacagactca gaagggtcaa ctaactttgg
    12781 aacaggctga actgactagc caacttcgtt ttgaacagac agatctgaga cgtttgcaag
    12841 aagaaaaagt ggttgcagat aaggaaattt ctcttttaga ggatatgatt gaccagaagc
    12901 tagaagctct tgaagatact agtattgaga ttcttgaaga acaattgcag gccgcatctg
    12961 ataagcaaaa ccagaccaat cagattctaa ttcgcttaaa gtttgaattg gaagatattg
    13021 atgggcagtt tgaagacttg gaagaaagat tgcaacaagc tcggactaag aatgatgatt
    13081 tgattcgtaa gcaggctaag ttagaggcgg attgtgagca agcgggcgat aaattacgga
    13141 ctctacttgg taatttgacc gagcatttta aattgagttt tgaagctgcc cagtctcagg
    13201 ccaaggaagt tgagaatctt gcagttgctg aacaaagcct caaagaccta gaaagagcta
    13261 ttcgtgcttt gggtccagtc aatttggatg ctattgagca gtatgacgag gttaataatc
    13321 gccttacctt cctcaatgaa caacggacag atattttgtc ggcgcgtgat ttgctcttga
    13381 ataccattca tgaaatggat gatgaagtca aagagcgctt caaggttact tttgaagcta
    13441 ttcgtgaaag ctttaagcag acattcaaac aaatgtttgg cggtggttca gcggatttga
    13501 ttttgacaga aggtgaccta ttgactgcgg gtgtggagat ttcagttcaa cctccgggta
    13561 agaaaattca atctctcaat cttatgtcag gtggcgaaaa agccctttcg gccctcgctc
    13621 tgctattttc cattatccgt gttaagacca ttccttttgt tattctcgat gaggtggagg
    13681 cagccttgga tgaggccaat gtcaaacgtt ttggcgacta cctcaaccgt tttgataagg
    13741 agagtcagtt tatcgtcgtg acccaccgta aagggaccat ggcggcagcg gatgccatgt
    13801 atggggtgac catgcaggaa tctggcgtgt ccaagattgt ttccgttaag ctgaaagatg
    13861 tggagaaatt atgattaaca aaatttacgc cagtgatatg gatggtacct ttttacgaga
    13921 agaccatagt tttgataagg aacggtttcg tcgtatactg aatcagttta aggaaaaagg
    13981 ttatttattt gtggcagcaa gtgggcgatc tatgcaaagt ctgaaacttg tttttgaaga
    14041 ttttgtagat gaaatcggtt ttgtggcaga aaatggttca atcgttgaat atcaggggca
    14101 gactatcttt atggatgatc caattagtcc tgaaatctat ctgccgatta tcgcgggtat
    14161 tgatgcaggt ccctttggta gcagtcagtc catggtgctg tctggcctcg aaaactttta
    14221 tttattaaaa aatgctgagc ctcagttttt agaagcgatg acgaattatt acgcacattt
    14281 tcgattggta gacacatttg aagaagttcg agaagagatt ataaaaatca atgccaaatt
    14341 ttctcctgag gaaatggacg atgccagacg atggttaaat gatacatttg aaggtgtaac
    14401 ggccatgaca actggttttg acaatattga tataattcca aatggttcaa ataaatcagt
    14461 tggattgagc catttgtgtc atcattttgg aattaccggt caggatgtag tggcatttgg
    14521 tgataatcaa aatgatttgg atatgtttaa ctttgcaggt ctggctcttg cgacagaaaa
    14581 tgcaagagag gaagtgaaag ctcgggcaga tttgatgatt ggtcattgca atgatggagc
    14641 agtattagcc tatttagagg aggaagtaaa tggcaattaa gttgattgca ttggatttag
    14701 atggaacact tctgacatcg gacaaacgga tttcagaagc taataaaaag gccttgcagg
    14761 tagcgcgtga gcgtggagtt tatgtggttc tgacaacagg gcgtccttta caagctatcg
    14821 gtacttttct agaagaacta gatctgctag gggagaagca atattcgatt gcatttaacg
    14881 gtggtctagt ccaggaaaat acaggtcgag ttttggataa ggttggcttc accattgacg
    14941 atgtgcgcac catccgtcgt gtgacaaatg acttggacct acctctggat gttctctatg
    15001 gtggcgatat ctattctcta ccatcagccc atgaatcact ttatctaact tgtaatccat
    15061 tgcttaataa aattgtggca agcgatgagg aaatgccaga agattttgtc tacaataagg
    15121 cagtttcagc tgtggcagca gactttttgg atggacaaat tccaaaaatt cctgcggaac
    15181 ttcacgagcg cttcgagatt ttcaaatcgc gtgatattct gctcgaatgg agtccaaaag
    15241 gtgttcataa ggcaaatggt ctggccaagt tgattggaca tctggggatt gatcagtcag
    15301 aagttatggc ttgtggcgat gaagggaatg atctttcgat gattgaatgg gctggtctgg
    15361 gtgttgctat ggctaatgcg actgatgaaa tcaagtctgc ggccaacctt gtcctgccaa
    15421 aaaacaatga tgaagatggc attgcttggg tcatcgagca ctatgtattg aatgaggact
    15481 agtatgggat tatttgatcg attatttgga caaaaaaaac aagaggagca agtcgctctt
    15541 gttgaagaca tagaaacgga gcagtcggct tctagcgaag atacagtaac tactgaagtt
    15601 gagtcagcta ctcaagaaat ggaagagttc cagtctgaag tagcagagcc tgaattgaca
    15661 gccgaacaag aagctcagaa acaacggacc ttagacatga tggctcaata ttatgcagcc
    15721 aaagaagccg ctgcagctcg tgttaaggca gcgcaggaac aaggctttga ccctgccatt
    15781 caaaccaagg tcaaagaaga gcctgttgag gaagtggctc cagtagagca agaaagtgag
    15841 caggacaagt accagcgtac cttgaaaaag acacgtacag gctttggggc aaggctcaat
    15901 gaattctttg ccaatttccg ttcggttgat gaagaatttt tcgaagagtt ggaagaaatg
    15961 ctgattttgt cagacgtagg tgtacaggtt gcctctacgc tgacagagga gctccgctat
    16021 gaagccacgc tacaaaatgc caagaagaca gatgaactcc gtcgtgttat catcgaaaaa
    16081 ttggtggata tttacgaaaa agatggtcaa ttcagcgagc aaatcaattt ccaagatgat
    16141 ttgacagtca tgctctttgt cggtgtcaac ggtgttggga agacgacgtc tatcggtaag
    16201 ttagcctaca agtacaagca ggctggcaag aaggttatgt tggtcgcagc ggacaccttc
    16261 cgtgcgggtg cggtagccca gctggtcgag tgggggcgcc gtgtcgatgt gccagtggtc
    16321 acaggacctg aaaaatcaga tccagccagt gtggtctttg acggggtaaa acgtgccgtg
    16381 gcagaaggcg tggatattct catgattgac acagcaggtc gcttgcaaaa taaagaaaac
    16441 ctcatggcag agctggaaaa aatcggtcgc atcatcaaac ggactctccc tgatgcaccg
    16501 catgaaaccc tcttagcact agatgcttcc acaggtcaaa acgcccttag tcaagccaag
    16561 gaatttgcaa aaattacccc attgacaggt cttgtcttga ccaagctaga cggtacagca
    16621 aaaggtggtg ttgtccttgc catccgtcag gaactggata ttccagtcaa gctgattggt
    16681 ttcggtgaga aaattgatga catcggtgaa ttcaaatccg aagaattcat gcgtgggctt
    16741 ttggaaggat tggtatagaa aaaagtcgtt agttcaatga actagcgact ttttccatct
    16801 gtatgcagca gtgagtaaat agcaatatcc acaatgccac gattgtttcg agcggcttgg
    16861 cggagagtgc cttcgtaggt cataccgact ttttccatga cacgaccaga aggaggattt
    16921 tcactatcgt gaacagctgt aatccgtaaa cagcccactt cctccaacat agttaaatga
    16981 aacaaaaaca gtacatttat gat
//
LOCUS       DATUCG010000044        16930 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.045, whole genome shotgun sequence.
ACCESSION   DATUCG010000044 DATUCG010000000
VERSION     DATUCG010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16930
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.045"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(19..930)
                     /locus_tag="VCA20_001119"
     CDS             complement(19..930)
                     /locus_tag="VCA20_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEP1834957.1"
                     /translation="MRIQQLYYIIKIAETGSMNEAAKQLFITQPSLSNAVRDLEKEMK
                     IKIFFRNSKGITLTKDGMEFLSYARQIVEQTELLEDRYKNPNAKRQLFSVSSQHYAFV
                     VNAFVSLLKETEMEDYELFLRETRTWEIIDDVKNFRSEIGVLFLNHFNRDVLLKLLDD
                     NRLSYTTLFTAKPHVFVSKTNPLAQRKSITLDDLVDFPYLSYEQGIHNSFYFAEEILA
                     QEHHKKSIVVSDRATLFNLLIGLDGYTIATGILNSNLNGDNIVSIPLEFDDEIELVYI
                     QHEKALLSDMGEKFINYLLEEVKFDQK"
     gene            1027..1917
                     /locus_tag="VCA20_001120"
     CDS             1027..1917
                     /locus_tag="VCA20_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEP1834958.1"
                     /translation="MINRANTAFFSCNLASQQGFFSISTFLFSVSLLYRIGEKMILKE
                     QMLLVDQVQLAPRIFAMTLQGDMVQDMKMGQFIHIRVPDDSMLLRRPISISEIDRNKS
                     QCRIIYRVEGQGTEVFSKMTVGQYLDVMGPLGKGFEVDFLKEGARILIIGGGIGVPPL
                     VEVAKQAANRGAKVTSVIGFATKEAVILEEELASYGMVYVTTDDGSYGRKGNVATVVE
                     ELTNEFAAIYSCGAPAMMNYVDQRFQEHPHAYISLEARMACGMGACYACVVKPKEGQE
                     HENKRVCKEGPVFATGSLIL"
     gene            1927..2868
                     /locus_tag="VCA20_001121"
     CDS             1927..2868
                     /locus_tag="VCA20_001121"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEP1834959.1"
                     /translation="MTKRLAISLPGLDLKNPIIPASGCFGFGQEYADYYDLNQLGSIM
                     INATTRHPRYGNATPRVAETPAGMLNAIGLQNPGIEAVLSEKLPWLEQHFPDLPIIAN
                     VAGFSNEEYAYVSGKISKAPNVKAIELNISCPNVDHGNNGLLIGQVPELAYQAVKAAV
                     EASSIPVYVKLTPSVADITLLAKAAEDAGATGLTMINTLVGMRFNLKTRQPILANGTG
                     GMSGPVVFPVALKLIRQVAQMTDLPIIGMGGVDTADKAIEMMVAGASAIGVGTANFTD
                     PFACPKIIQDLPKRMEVYGIDSLEGLRREIRHSLGKR"
     gene            3033..3728
                     /gene="pyrF"
                     /locus_tag="VCA20_001122"
     CDS             3033..3728
                     /gene="pyrF"
                     /locus_tag="VCA20_001122"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEP1834960.1"
                     /translation="MKETRPIIALDFAGKEEVQSFLEQFPPDEKLYVKVGMELYYAEG
                     PGIINYLKGCGHSIFLDLKLHDIPNTVESAMKVLAKLGVDMTNVHAAGGVEMMRAARR
                     GLGKDAVLIAVTQLTSTSEEQMRTDQNIQTSLQEAVVHYAQRAQEAGLDGVVCSAHEV
                     ALIKDATSSDFVCLTPGIRPTGDEVGDQKRVMTPADAARIGSDHIVVGRPITKSEHPY
                     QTYLSIKEEWNRG"
     gene            3721..4047
                     /locus_tag="VCA20_001123"
     CDS             3721..4047
                     /locus_tag="VCA20_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEP1834961.1"
                     /translation="MDKIYQVIIDRPIGYIDKFGNQYPINYGYVPNLLAGDGEEQDVY
                     IISKAVRQPLTAFEGKLVAIIHRRDDVEDKWVLTGLDEKLTVTEIKEKTNFIEQYFDS
                     WIEMVD"
     gene            4185..4817
                     /locus_tag="VCA20_001124"
     CDS             4185..4817
                     /locus_tag="VCA20_001124"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEP1834962.1"
                     /translation="MTLATEIASHLLDIKAVHLRPEKPFTWASDIKSPIYTDNRITLS
                     YPETRNLIEDGFVQRIEEEFPEVEVIAGTATAGIPHGAIIADRMNLPFAYIRSKPKDH
                     GAGNQLEGRIVKGQKMVVVEDLISTGGSVLDAVAAAEREGADVIGVVAIFTYELPKAD
                     RNFDNAGVKLVTLSNYSELIKVAKVQGYINADGLNLLKKFRQNQETWLED"
     gene            5181..6143
                     /locus_tag="VCA20_001125"
     CDS             5181..6143
                     /locus_tag="VCA20_001125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834963.1"
                     /translation="MTSQVNHWNLWNGRFVAHTMVQIVLMFPNIVFDILNTAAFLVLG
                     LFVERFVVRLDSTKKQGNPELLVLIYLLLWWFLPEIGKTVLWVSGAGNYLWTSLIYLS
                     FFYRILFKSKTSIFDGVSLGLLGFLAGGTNENAAPSVLLMGALYLFYEVVVNKNFQLQ
                     KWFSLFYMFAGFLLMMSSPGSQERGSAQFNIDFIITQLLTILRYQRSHFGILYAGTII
                     MLVFLLYRRMVDKEKIVKIFVFIIGHFASIYCLVFSPEIPDRVYFCSTIFLIIPSLYM
                     FFILLKETPVKFFINKVLCAGILLTTMTYCFVFWDNYTTYKQVK"
     gene            6425..7351
                     /locus_tag="VCA20_001126"
     CDS             6425..7351
                     /locus_tag="VCA20_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEP1834964.1"
                     /translation="MISVIVPCFNEEEAIPYFYDAMEKVRKEMGEQFEYIFVNDGSKD
                     GTLTVLRQLSGQDRAVRYLSFSRNFGKEAALYAGLQAAQGELVTVMDVDLQDPPEMLM
                     EMKAMLDGNPDLDCVGTRRVSRDGEPPIRSFFAKLFYKLMNKISQVEVVDGARDFRLM
                     RRHMVDAILSVSEYNRFSKGIFAWVGFETEYLPYENVERVAGETSWSFWKLLSYSIEG
                     IINFSDTPLNIASYTGFFTFLLSLVLMVFVFFKTLVFGDPTIGWPSTICIILFLGGLQ
                     LMTIGILGKYLAKVFLETKKRPIYIVKEKSSN"
     gene            7361..8014
                     /locus_tag="VCA20_001127"
     CDS             7361..8014
                     /locus_tag="VCA20_001127"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEP1834965.1"
                     /translation="MEHSAWHELIKAQLPEHYFGKINQFLDHVYVGGTVYPPREKVFA
                     AIQTTDLEEVKVVILGQDPYHGPGQAQGLSFSVPNSVPAPPSLQNILKELTDDIGVKQ
                     DHDLTAWAEQGVLLLNACLTVPAGQANGHAGQIWEPFTDAVIKVVNSLDQPVVYILWG
                     AYARKKKALITNPKHLVIESAHPSPLSAYRGFFGSKPFSQVNAYLTSQGRTGIDWLR"
     gene            8054..9325
                     /locus_tag="VCA20_001128"
     CDS             8054..9325
                     /locus_tag="VCA20_001128"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEP1834966.1"
                     /translation="MLLVKNGRVLDPQSGLDKVCDILMDGSKIVKIAESISEDVEQEV
                     IDATGLVVAPGLVDVHVHFREPGQTHKEDIHTGALSAVAGGFTSVVMMANTNPTISNV
                     ETLKGVLDSAKKEAIHIYSVATITDHFDGQNLTDFPALLEAGAVAFSDDGIPLTNAGV
                     VRKAMKLAKEQGCLISLHEEDPDLNGVLGLNEQVAEKHFHVCGATGVAEYSMIARDVM
                     IAYETGARVHIQHLSKAESVKVVEFVQGLGANVTAEVAPQHFSRTEELLLTKGANAKM
                     NPPLRLESDRLAVIDGLKSGVISVIATDHAPHHADEKNVEDLTQAPSGMTGLETSLSL
                     GLTHLVEVGHLTLVQLLEKMTINPAKLYGLDAGYLAENGPADLVIFDSNANRLVTADF
                     ASKSANSPFVGDSLKGQVLYTIANGQIVFGK"
     gene            9455..11569
                     /locus_tag="VCA20_001129"
     CDS             9455..11569
                     /locus_tag="VCA20_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HEP1834967.1"
                     /translation="MKKKKILLPVMSTLLLAPFVLAQQVQAAETTTAATTTNQPATTD
                     ATVTVPVTSVENVATEETVVPAAEETVEAVIIHTNDVHGRILEEKNVIGDAKAAAVIE
                     EERAKVENTIVVDAGDAFQGLPISNSTKGEDRANIMNQVGYDAMAVGNHEFDFGMDQA
                     IKYKETLNFPLLSANTYVNGARVFEASTIVDKTPTVVGDEFVVIGVTTPETATKTHPK
                     NVEGVTFTDPVTEVNKVIDEVEARALADNRVYKNYIVLAHLGVDSTTPVEWRGSTLAE
                     ALSKNSKLAGKRVIVIDGHSHTVEATTYGDNVTYNQTGSYLNNIGKVTLKSDKLLGEA
                     SLISAADTKNVTPNAKIAALVDEIKAKYEAENAQVVIENNPVELNGDRSNVRVRETNL
                     GNAVTDAIYAYGQTGFSNKTSLAVTNGGGLRATIAKDQPVTKGDIIAVLPFGNIVSQI
                     TVTGQQIYDMFTKSLSSTLQVNPETGEMLLDENGMPLFEASGGFLHISGANVFYDPTL
                     PVEERVLLIGILNPETGEYDALDLEKTYYLATNDFLAAGGDGYTMLGGAREEGPSMDS
                     VFAEYLKTADLSAYEVVNPYSRIIPVNSSIDTDEDGYPDFIEILLDTDPENPASNPET
                     VPAENTDSPSNQAQNTSATDKKAPVDSPKVGDKKPEVASPAKTTKAGVLPNTGDQMNL
                     TLSLFGLGLAGLAMAVGRRKEN"
     gene            11621..12267
                     /locus_tag="VCA20_001130"
                     /pseudo
     CDS             11621..12267
                     /locus_tag="VCA20_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735825.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
     gene            12319..12807
                     /locus_tag="VCA20_001131"
     CDS             12319..12807
                     /locus_tag="VCA20_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEP1834968.1"
                     /translation="MTVDIERYKQLAQQKQGEHRKFLANLKKKAPKNLDRIVQDIHAE
                     VFSEIDCTECANCCKTLGPLFTEADITRIAKHLRMKLPVFEDMYLRVDEDGDKVFQSM
                     PCPFLGGDNLCSIYDARPKACREFPHTDRKKIYQINNLTIKNTLICPAAYLFVEKLRE
                     RI"
     gene            12907..13758
                     /gene="ylqF"
                     /locus_tag="VCA20_001132"
     CDS             12907..13758
                     /gene="ylqF"
                     /locus_tag="VCA20_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEP1834969.1"
                     /translation="MAIIQWFPGHMSKARRQVQENIKHVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLMILNKVDLADPVRTKEWQSYFEGQGIRTLAINSKEQSTVKKVTDAAK
                     SLMKEKIERLRERGIQKETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKSNKDLEILDTPGILWPKFEDQEVGLKLALTGAIKDQLLPMDEVTIFGLDYFKANY
                     PERLQERFKGMDLEQETPELIMDWTKKLGFRDDYDRFYNLFVKDVRDGRLGTYTLDRV
                     SDLDGND"
     gene            13745..14518
                     /locus_tag="VCA20_001133"
     CDS             13745..14518
                     /locus_tag="VCA20_001133"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEP1834970.1"
                     /translation="MATIKEVTALLAQVDSLDSPVWDQLAQDERAGVQAAIKKRQKEL
                     EKEAAEDARLEAMLFYEKALYDNGVEFIAGIDEVGRGPLAGPVVAAAVILPKGCKIRY
                     LNDSKKIPKSKHEAIYQEVMERAVAVGVGVKDAAVIDQVNIYEATKLAMLEALGQLSQ
                     EPEHLLIDAMKLDTPLPQTSIIKGDANSLSIAAASIVAKVTRDKMMADYNKEFSGYGF
                     AKNAGYGTAEHLEGLNKLGITPIHRKTFEPIKSMVAGGN"
     gene            14521..15531
                     /locus_tag="VCA20_001134"
     CDS             14521..15531
                     /locus_tag="VCA20_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015445222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEP1834971.1"
                     /translation="MTVFFWLFLVAIGLFALVYFWERRSIFISLFALNILVWALAWII
                     STGILENNEILRLLAIAVAMVLFFALLSGPFVLLFTLYLNGFQILKREGVRFHNFLSM
                     GLAVALTFYLFIAPFVVQSLSGISLLNMVFVYVGFLVSYAIIISMLYTTSSFVNLVNF
                     FPGKLDYVVVLGAGLIGDKVTPLLASRIEKGIAVYRKHPGSKLIMSGGQGPDEIIAEG
                     QAMANYALEQGIPVEDIVIENQSTNTEENLKFSYALMKPGSRFALVTNYYHVFRALLL
                     ARKLKIKCIGYGAKTRFYFSLNAFIREFVGYVVMSRKVHLVFLSIVSALYLIGVIISL
                     MH"
     gene            complement(15564..16214)
                     /locus_tag="VCA20_001135"
     CDS             complement(15564..16214)
                     /locus_tag="VCA20_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEP1834972.1"
                     /translation="MNPSLKLSLQLSFGEEVANSVTHAVGALLMLVLLPVTAIHSYQD
                     YNLTAVIGMSIFVISLFLMFLSSSIYHSMPYASTHKYILRIIDHSMIYIAIAGSYTPV
                     ALSLVGGWLGYLIVGLQWGLTIFGILYKIFAKKINEKFSLFLYLFMGWLVVFILPAIL
                     QKTGMAFGILMLAGGLSYTIGALFYAKKKPYFHMIWHLFILLASALQYIAIVYFML"
     gene            complement(16204..16755)
                     /locus_tag="VCA20_001136"
     CDS             complement(16204..16755)
                     /locus_tag="VCA20_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEP1834973.1"
                     /translation="MKQTIPFWHELPDLDLYLDQVLLYVNQVVNSQESLEQKSLYSMK
                     INSSLGNETDDRTGVHQGKVTTDNIDFRRVLTAAMINNYVKHKQLEKPIKKKYQKQQV
                     ARLIALTILKNVFSIQEISQTLNLLLQSSNSETLYNHFVDCMRNEESDETPEIIRYAC
                     QSVKLYYKTRQLTMELERSHHES"
BASE COUNT         4941 a         3124 c         3892 g         4973 t
ORIGIN      
        1 atctatataa aatggtattt atttttggtc gaatttcacc tcttcaagta ggtaattgat
       61 gaatttttct cccatgtcgg acagcaatgc cttttcgtgt tgaatataga ccaattcgat
      121 ttcatcatca aattccagag gaattgatac aatattatct ccgttaagat ttgaattgag
      181 gatacctgtc gcgattgtat agccatctag accgattaag agattgaaga gtgtggcacg
      241 gtcagatacg acaattgatt ttttatggtg ttcctgggct agaatttctt ctgcaaagta
      301 gaaggagttg tgaatacctt gctcgtagct gagatagggg aaatctacca aatcatcgag
      361 agtgattgac tttctttgtg ccaaaggatt ggttttgctg acaaatacat gtggtttggc
      421 agtgaagaga gttgtatagg atagtcgatt gtcatccaaa agtttcagca gaacatcacg
      481 gttgaaatgg ttgaggaaaa gcactccgat ttctgagcgg aaattcttaa cgtcatcaat
      541 gatttcccag gtccgtgttt cacgtaggaa gagttcgtaa tcctccattt cagtctcttt
      601 caaaagcgag acaaaggcgt taaccacaaa ggcatagtgt tgggaagata cgctgaagag
      661 ttggcgtttt gcattcggat ttttatagcg atcttctaaa agctctgtct gttcaacaat
      721 ttgacgagcg taggaaagaa actccatgcc atcctttgtc agggtaatgc ccttagaatt
      781 acggaaaaag attttaattt tcatttcttt ttctaggtct cgtacagcgt tggaaaggct
      841 gggttgggta atgaagagtt gtttggcagc ctcattcata ctgcctgttt ctgcaatttt
      901 gataatatag tataattgtt ggattctcat acctctattg taacatgatt tgacaaatta
      961 ttctaaaaaa tgtataatgc tgaaaatata tcctttaact gagtccagag aggcgaagaa
     1021 gggaacgtga taaatagggc aaatactgcc ttcttttcgt gtaaccttgc tagccagcaa
     1081 ggttttttct ctatatcgac tttcttgttt tctgtgtcat tgttatatag aataggagaa
     1141 aagatgattt taaaagaaca gatgttactg gtggaccaag tccaactagc accacgaatt
     1201 tttgctatga ccttgcaggg tgatatggtc caagatatga aaatggggca atttatccat
     1261 atacgtgttc cagatgattc catgctcttg cggagaccga tttctatttc cgagattgat
     1321 cggaataagt ctcaatgtag gattatttac cgagtggaag gtcaagggac ggaagtcttt
     1381 tcgaaaatga ctgttgggca gtatttggat gttatgggac cgcttgggaa agggtttgaa
     1441 gtagactttc tcaaagaagg tgcccgcatt ctcatcattg gcgggggaat cggtgttcct
     1501 cccttggtgg aagtagccaa acaggcggcg aaccgtggcg ccaaagtcac gtcagtcatt
     1561 ggctttgcca ccaaagaggc cgtcattttg gaagaagaac tggcaagtta tgggatggtc
     1621 tatgtcacaa cggatgatgg ttcctatggg cgtaagggga atgtcgcaac ggtcgttgaa
     1681 gagctgacaa acgagtttgc agccatttac tcatgtgggg cgccagctat gatgaactat
     1741 gttgaccaac gctttcagga acatcctcat gcctacattt cactggaggc taggatggct
     1801 tgcgggatgg gggcgtgcta tgcctgtgtt gtcaaaccca aagagggaca agaacacgag
     1861 aacaagcgtg tctgcaagga aggtccagtc tttgcgactg gtagtctgat tttataggag
     1921 gttgaaatga caaaacgatt agcgatttcc ttaccaggct tggacttgaa aaatcccatc
     1981 attccagcct ctggttgctt cggttttggt caggagtatg cagattatta tgaccttaac
     2041 cagcttggat ccattatgat taatgcgact acccgtcatc ctcgttatgg taatgcgact
     2101 ccccgtgtag ccgagacacc agcaggtatg cttaatgcca ttggacttca aaatccgggt
     2161 attgaggcgg tcttatctga aaaattacca tggttagagc aacatttccc agacttgcca
     2221 atcattgcca atgtggctgg tttttccaat gaagaatatg cctatgtatc agggaaaatt
     2281 tcaaaagctc ctaatgtaaa agcaattgaa ctaaatattt cctgtcccaa tgtggaccat
     2341 ggcaacaatg gtttgctaat tggtcaggtg ccagaattag cctatcaagc agtaaaagca
     2401 gcagtcgaag cctcatctat acctgtttac gttaagctga caccaagtgt ggcagacata
     2461 accctgttgg ccaaggctgc tgaggatgct ggagcgactg gcttgacgat gataaatacc
     2521 ttggttggta tgcgattcaa tctcaaaact cgacaaccaa tcttagcaaa tggtacagga
     2581 ggaatgtcag gtccagtagt ttttccggtt gctctcaaac tcattcgtca ggttgctcag
     2641 atgactgatt taccgattat tggtatgggt ggtgtggata cggcggacaa ggctatagag
     2701 atgatggttg caggagcttc agccattggg gtaggaacag ccaactttac agacccattt
     2761 gcttgtccaa agattattca agatttgcca aaacgcatgg aagtttatgg tattgatagt
     2821 ttggaaggtt tacgccgaga aatcagacat agcttgggta agcgttgaca aaggtaaaat
     2881 aaaagagtaa tataaagaaa atatctcctt taattaagtc cagagaggct aggaaggtgt
     2941 catgtaatat gcttgaaagg tgattttcaa gctgaattcg tgcacatccc tgaccgattt
     3001 ggcagggatt tttgtgtaaa tgaaaggatt ttatgaaaga aacaagacca attattgccc
     3061 ttgactttgc aggtaaagaa gaagttcagt cttttctgga acagttccca ccagatgaga
     3121 aactctatgt aaaggttgga atggagttgt attatgcgga gggaccaggt attatcaatt
     3181 atttgaaagg ctgcggtcac agtatttttc tagatttgaa actgcatgat attcctaata
     3241 ccgtcgagtc tgctatgaaa gtcttggcaa aactcggtgt ggatatgacc aacgttcacg
     3301 ctgctggtgg tgttgagatg atgcgggcag cacgaagggg attgggtaag gatgctgttt
     3361 taatagcggt cactcagttg acctctacct cagaggagca gatgagaaca gaccagaata
     3421 tacagactag tttgcaggaa gcggttgttc attatgctca aagagcacaa gaagctggtc
     3481 ttgatggggt ggtttgttca gctcatgagg tggctttgat taaagatgct acgagttctg
     3541 attttgtgtg cctgacgcca gggattcgtc cgactggcga tgaagtaggc gatcagaaac
     3601 gagttatgac accagcagat gctgctcgaa tcggtagtga tcacatcgta gtaggtcgcc
     3661 cgattacaaa atcagaacat ccttaccaga cctatttgtc tattaaagag gagtggaatc
     3721 gtggataaga tttatcaggt tataatcgat agacctatcg ggtatattga taaatttggc
     3781 aaccagtacc caatcaatta cggctatgtt ccaaatctat tggctggaga tggtgaagag
     3841 caagatgttt acatcatttc caaagctgtt cggcagccct tgacagcttt cgaggggaaa
     3901 ttggtcgcga ttatccatcg cagagatgat gttgaggata agtgggttct gacaggcctt
     3961 gatgaaaaac ttaccgtgac agaaataaaa gagaaaacca atttcattga acagtatttt
     4021 gattcatgga ttgaaatggt ggactaggag aagtctgtaa gaattcaacg gggataaata
     4081 tagttttttg tagggttcat agattgaacc aatactttag gagtgacagg ctattcggcg
     4141 ggatggtcat gttggaatgg tacgcattta gaaggaggtc tattatgaca ctagcaacag
     4201 aaatcgcatc acatttgttg gacattaagg cagttcatct gcgtccagaa aagccattta
     4261 catgggcatc agatatcaaa tcacctatct atacagataa tcgtattaca ctatcttatc
     4321 ctgaaactcg aaatttgatt gaagatggct ttgttcagcg tatcgaagaa gaatttcctg
     4381 aggttgaggt gattgccgga actgccacag caggtattcc tcatggtgct atcattgcag
     4441 atcggatgaa tttgccattt gcttatatcc gtagcaaacc aaaagaccat ggtgcaggga
     4501 atcaattaga aggtcgcatc gtcaagggac aaaaaatggt tgtcgttgaa gacctgattt
     4561 ctactggagg gtctgttcta gatgccgtag cagcagctga gcgtgaaggc gctgacgtta
     4621 ttggtgttgt ggcaatcttt acctatgaat tgccaaaggc agatcgtaat tttgataatg
     4681 ctggcgtgaa attggtgacg cttagcaatt attcagagtt aatcaaggtc gcaaaagtac
     4741 agggatatat caatgcagat ggtttgaatc tgcttaaaaa gttccgtcaa aatcaagaaa
     4801 cttggttaga agattaggaa accagtaaaa attattgaag taaatgattg attataaaga
     4861 agcaatttac taaacatttt atctaagtca ttaaatgcaa tttttagagg agatacagtg
     4921 gagattaaaa tgaatatata tataatctat gttagatgtt cgttgaaaca ttctggaatg
     4981 ttaggagaat gtttatgcga acgctagtta ttactaaacc aaagattaaa cggataattg
     5041 tttgtgtaat actgtttttg gcaattttct ttctgaatag aatgacacta ttcacgtcgg
     5101 atgattttac ctatcatttt atctataagg gatactttcc tacaaaggat gtagaacgag
     5161 tcaattcctt gttttctata ttgacctctc aagtaaatca ttggaattta tggaatggtc
     5221 gatttgttgc acatacgatg gtgcaaatcg ttcttatgtt ccctaacatc gttttcgata
     5281 ttctgaatac ggcggcattc ttagtactag gtttgtttgt cgaaagattt gtagtcagac
     5341 tagattctac taaaaaacag gggaatcctg aactgcttgt actaatttat ctattattat
     5401 ggtggttttt acccgaaatt ggaaaaacag ttttatgggt ttcaggggct ggaaactatt
     5461 tatggacatc tttaatttat cttagtttct tttatagaat tttatttaaa agcaaaacaa
     5521 gtatatttga tggagtttct ttaggattat tgggttttct tgctggagga acaaatgaaa
     5581 atgcagctcc tagcgtgctt ttaatgggtg ccttgtatct tttttatgag gtagttgtta
     5641 ataagaattt tcaattacag aagtggtttt ctttgtttta catgtttgca ggatttctat
     5701 taatgatgtc ctcacctggt tctcaagagc gtggatcagc ccaattcaat atagatttta
     5761 ttatcaccca gttattgact atcttacgtt atcaacgttc acattttggt attttatatg
     5821 ctggaactat tataatgttg gtattccttt tatataggag aatggtcgat aaagagaaaa
     5881 ttgtcaagat ctttgtgttt atcataggtc atttcgcttc tatttattgc ttggtttttt
     5941 ctccggagat accagatagg gtttattttt gttcgacaat tttcctaatc ataccgtctt
     6001 tatatatgtt tttcatcctt ttaaaagaga ctccagttaa attttttata aacaaagttt
     6061 tatgtgcagg tatattatta acaacgatga cctattgttt tgttttttgg gataactaca
     6121 caacatataa acaagtaaaa taacaatatg ctcaaatatt tttagcaaaa gaacagggag
     6181 tggacacggt ggtcttgaaa aggttgactc caagtagaac tttatataat gcctataatg
     6241 ggactgctaa tttaggggca agtgaacaag actggtttaa tagttggatg gctgtgtatt
     6301 tcggtattga taaaatacaa tcagttgatt gaagtatacc catatcctag cagttttttt
     6361 gcaaaatggt ataatgggaa ttaagtatgt ttgacataca aattgaccgt atgaggagaa
     6421 gaacatgatt tcagttatcg tgccttgttt taatgaagaa gaggcaattc cctattttta
     6481 cgatgctatg gagaaagttc gtaaggagat gggggaacaa tttgagtata tttttgtcaa
     6541 tgatggttca aaggatggga cattaacggt tttacgccaa ttgtctggtc aagaccgagc
     6601 tgttcgctat ctttcctttt caaggaattt cgggaaagaa gctgctctct atgcaggtct
     6661 acaagctgcg caaggtgagc tagtgactgt catggatgtt gatttacaag atccacctga
     6721 aatgttgatg gaaatgaagg ctatgttgga tggaaatcca gatttagatt gcgttggaac
     6781 ccgtcgtgtc agtcgagatg gtgagccacc gattcgtagc ttttttgcaa aattattcta
     6841 caaattgatg aataaaatca gccaggtcga ggtagtagac ggtgcccgtg atttccgtct
     6901 catgcgtcgt cacatggtag atgccattct atctgtgtct gaatacaatc gcttttcaaa
     6961 aggaattttt gcttgggtag gattcgaaac agaatacttg ccatatgaaa atgttgagag
     7021 ggtcgctggt gaaactagtt ggtctttctg gaaattgctt tcttattcta ttgaaggaat
     7081 catcaatttt tcagatacac ctttgaatat tgcctcctat acaggttttt tcaccttcct
     7141 attgtcgcta gttctaatgg tttttgtgtt tttcaaaacc ttggtgttcg gagatcctac
     7201 aattggctgg ccgtcgacta tttgtatcat cctatttttg ggaggtctac agttgatgac
     7261 tattggtatt ctcggtaaat atttggcaaa agttttcttg gaaacgaaga aacgtccgat
     7321 ttatattgtt aaagagaaaa gttcaaacta ggagcagttt atggaacatt cagcttggca
     7381 cgaattgata aaggcacagt tgccagagca ttattttggt aaaatcaatc agtttttaga
     7441 tcatgtttat gttggtggaa ccgtttatcc gcctcgtgag aaggtatttg ctgccattca
     7501 aacaacggat ttggaagagg ttaaagtagt tattttggga caggatccct atcacggacc
     7561 tggtcaggca cagggcttgt cattttctgt tccgaactca gtaccagctc cgccatctct
     7621 tcaaaacatc ttaaaagaat tgacggatga tattggcgtc aaacaagatc atgatttgac
     7681 agcatgggcg gaacaaggtg tgcttttgct caatgcttgt ttaactgttc cagcaggtca
     7741 ggcaaatgga catgcagggc aaatctggga gccttttacg gacgctgtta ttaaggtggt
     7801 caatagctta gaccagcctg ttgtctatat tctttggggt gcttatgctc gcaagaaaaa
     7861 ggcgttgata accaatccaa aacatctggt tatcgaatct gctcatccaa gtcctctttc
     7921 agcctatcga ggtttctttg gaagcaagcc cttctcacaa gtaaatgcct acttgacttc
     7981 acaaggcaga acaggaattg attggctgcg atagtagatg gtaatggctt tggtactaca
     8041 aaggagaata tgaatgttat tagtgaaaaa tggtcgtgta ttggatccgc aatccggttt
     8101 agacaaggtt tgcgacatct tgatggacgg aagtaagatt gttaagattg cggagtcgat
     8161 ttcagaagat gttgagcaag aagtgattga tgcgacagga ttagtcgttg ctcctggttt
     8221 ggtcgatgtt catgtgcatt ttcgggagcc aggtcagacc cataaagaag atattcatac
     8281 aggagctctt tctgcagtag cgggtggttt tacttctgtt gtcatgatgg ctaacactaa
     8341 tccgacgatt tcaaatgttg aaaccttaaa aggagtattg gattccgcta aaaaagaagc
     8401 catccatatt tatagtgtgg caaccattac agatcatttc gatgggcaaa atctgaccga
     8461 ttttcctgct ttgctggaag ctggagcggt ggccttttcg gacgacggta ttcctttgac
     8521 aaatgctggc gtggttagaa aagctatgaa attggccaaa gagcagggct gtctcatcag
     8581 tctgcatgaa gaagaccctg acctgaatgg tgtcttggga ctcaacgagc aagtagctga
     8641 aaaacatttt catgtttgtg gagcaactgg cgtagcagaa tacagcatga ttgctcgcga
     8701 tgtcatgatt gcctatgaaa cgggtgcgcg tgttcatatc cagcatctat ccaaagccga
     8761 gtcggtcaag gtggttgaat ttgttcaagg tctcggagca aacgtcacgg cagaagtagc
     8821 accccaacat ttttcacgaa cagaagaatt acttctaaca aaaggtgcca atgctaagat
     8881 gaacccgcca ctacgattag aaagtgatcg cttggctgtg attgatgggt tgaaatctgg
     8941 tgttatctct gtcattgcga cggaccatgc acctcatcac gcagatgaga aaaatgtaga
     9001 ggaccttacg caagctcctt caggaatgac aggcttggaa acctcgcttt cacttggttt
     9061 gacgcatctg gtggaagtag ggcatctgac cctcgtgcaa ttgttggaga aaatgactat
     9121 taatccagcc aagctctatg gtcttgatgc tggctatctt gctgaaaatg gcccagctga
     9181 tcttgttatt tttgactcaa atgcaaatcg gttggtcaca gcggattttg cctcgaagtc
     9241 tgcaaattct ccgtttgttg gtgatagtct gaaaggacag gtcctttaca ccattgcaaa
     9301 tggacaaata gtatttggta aataggaaat gagcggatta gatggctaat tcgtttattt
     9361 cgaacctttt tgacatgtaa gcgattttaa tgtttttgtt ttttgttaaa aagtgtataa
     9421 tagagatata aaaatgccga agaaaggtct atttatgaaa aagaaaaaaa tcttgttgcc
     9481 tgtaatgtcc acactcttgt tggctccatt tgttttagct caacaagtac aagctgctga
     9541 gacaacgact gcagctacca caaccaatca gcctgcaacg actgatgcaa ctgttacggt
     9601 accagtaacg tcagttgaaa atgtagcaac ggaggaaact gttgtccctg ctgcggaaga
     9661 aacagtagag gcagtcatta tccacacaaa cgacgttcac ggtcgcattc ttgaagaaaa
     9721 gaatgtcatc ggtgacgcta aggctgcggc agtcattgaa gaagaacgtg ccaaagtaga
     9781 aaatacaatt gtagtagatg ctggtgatgc cttccaaggg cttccaattt ccaactctac
     9841 aaaaggtgaa gaccgcgcca atatcatgaa ccaagtcggc tatgatgcaa tggctgttgg
     9901 taaccatgaa tttgactttg ggatggatca agcaatcaag tacaaggaaa ccttgaactt
     9961 cccgctattg agtgctaaca cttacgttaa tggcgctcgt gtttttgaag cttcaactat
    10021 cgtcgataaa actccaactg ttgtaggtga tgaatttgta gttatcggtg taacaacacc
    10081 tgaaactgct acaaaaaccc atccgaaaaa tgttgaggga gtaacattta ccgacccagt
    10141 cacagaagtc aataaagtga ttgacgaagt ggaagcacgt gctctagcag ataatcgtgt
    10201 ttacaagaac tatatcgttc tagctcacct aggtgtcgat tctacaacgc ctgttgaatg
    10261 gagaggttca actcttgctg aagcactttc taaaaatagc aaattagctg gtaaacgtgt
    10321 tattgttatc gacggtcatt cccacacggt agaagcgact acctatggag ataacgttac
    10381 ttataatcaa acaggctctt acttgaacaa tatcggtaaa gtaaccttga aatccgataa
    10441 attattgggt gaagctagtt tgatttctgc tgcggatact aaaaatgtga cgccaaatgc
    10501 taaaatcgca gccttggttg atgaaattaa agcaaaatat gaagctgaaa atgctcaagt
    10561 agttatcgaa aataacccag tggaattaaa cggagaccgc tcaaacgtgc gtgttcgtga
    10621 aactaacttg gggaatgcag tgacggatgc gatttatgcc tatggtcaaa caggattttc
    10681 aaacaaaact agccttgctg tgactaacgg tggtggtcta cgtgcaacaa ttgctaaaga
    10741 tcagcctgtt acaaaaggtg atattattgc agtcttgcca tttggtaaca ttgtttctca
    10801 aatcactgtg actggtcagc aaatttatga tatgtttacc aagtctttat catctacatt
    10861 gcaagtgaat ccagaaactg gcgagatgct tttagatgaa aatggtatgc cactctttga
    10921 agctagtggt ggattcctac acatttcagg agcaaatgta ttctacgatc caactcttcc
    10981 tgtagaagaa cgtgttcttc ttatcggtat tcttaatcca gagactggcg aatatgacgc
    11041 cttggatctt gaaaaaacgt actatttagc aacaaatgac ttccttgctg ctggtggtga
    11101 tggctacaca atgcttggcg gagcacgtga agaaggtcct tcaatggaca gcgtgtttgc
    11161 tgaataccta aagacagctg acttatcagc ttatgaggtt gtcaacccat attcacgtat
    11221 cattcctgta aattcaagca ttgatacgga tgaagatggc tacccagact ttatcgaaat
    11281 tttattggat actgatccag aaaatccagc ctcaaatcca gaaactgttc cagccgaaaa
    11341 tacggattca ccatctaatc aagcgcaaaa tacttcagca acggacaaga aggccccagt
    11401 tgattctccg aaagtaggag ataaaaaacc agaagtagca agtccagcaa aaactaccaa
    11461 agcaggagtc ttgccaaata ctggtgacca gatgaatctg acgctcagcc tgtttggttt
    11521 aggtcttgca ggactggcca tggcagtagg tcgtagaaaa gaaaattaat caatcatctg
    11581 aaaacagggt cttcggactc tgtttttgac tggaggaaac atgcaaactg tatttcaata
    11641 tcaaaagagg gggatcgtga gcagattagt tcttacgttc tgcttagcaa ctccccacgt
    11701 cgtaaacaat tattgggatt tttacaacca caaatcgcat cagttgaagt ggatgagcgt
    11761 ggtatcgaag aacattttat gaaacaattc gctacagatg attttctcac tcgagctgca
    11821 aaaacttgtt gtgaaatttc caaagccaaa tcggacatgg atttggtaac tggtcatctc
    11881 tacatttctg cggatacaat cgtcgtgtca gatgggcgaa tttttaacaa acctcaagat
    11941 ttgacagaag ctgaaaccat gttacgttcc tattttggaa agagtcatca cgttgtaaca
    12001 tctgtctgcc tacggacaag acactcactg gatgtttttt atacggttgc acagattgat
    12061 tttgtcgact attatccagc ccttgaagag gttattcaat catatatccg tgaaaagcag
    12121 ccactggata aggccggtgc ctacggtatt caggagctgg ccccacgctt tgtagcaggt
    12181 atttctgggg atattcacac cattattggt ctacctgtag ccgaagtttc ccgcaggatt
    12241 ttttctgaag aaggttttga aaaatagcag agctcaatag ctagtttgtg gtataatagt
    12301 ggtcgaaaag gagagtttat gaccgtcgac attgaaagat acaagcaatt ggcccagcag
    12361 aagcaaggag aacatcgaaa atttctagca aatttgaaga aaaaagcacc taaaaatttg
    12421 gatagaattg ttcaagatat tcatgccgaa gtattttctg aaatcgactg tacagagtgt
    12481 gccaactgtt gtaaaacgtt gggaccactc tttacagaag cagatattac ccgaattgcc
    12541 aagcatttgc gcatgaaact tccagttttt gaggacatgt acttgcgtgt ggatgaggac
    12601 ggagataagg tatttcaatc catgccctgt cccttccttg gaggcgataa tctatgtagt
    12661 atctatgatg ctcgtcccaa agcctgtcga gaatttccgc atactgaccg caagaagatt
    12721 tatcaaatca acaatttaac aataaaaaat actctgattt gtcccgcagc ctatctcttt
    12781 gtcgaaaagt tacgggagag aatttaagag gaacaagtaa gacaaaatat tgattttctg
    12841 ctataataat aaaaaaacaa gccgttttta cggctttttt caatgaatca tatttaggag
    12901 gaccccatgg caattattca atggtttccg ggccacatgt caaaagctcg caggcaggtg
    12961 caggagaaca tcaagcacgt tgatttcgtg actattttgg tcgatgctcg cctgccccta
    13021 tctagccaaa atcctatgct gaccaagatt gtcggcgaca agcccaaact catgattctc
    13081 aacaaggtcg acttagcaga ccctgttcgt accaaagaat ggcagagcta ctttgaaggt
    13141 caaggcatcc gtacccttgc tatcaactca aaagagcaat cgacggtgaa aaaggtgacc
    13201 gacgcagcca agagcctgat gaaagagaag attgaacgcc tccgcgaaag agggattcaa
    13261 aaggagacct tgcgaaccat gattatcggt attccaaacg ctggaaaatc caccctcatg
    13321 aaccgtctgg ctggaaagaa aattgccgtt gtcggcaaca aaccaggtgt aaccaaaggc
    13381 caacagtggc tcaagtccaa taaagatctt gaaatccttg atacacctgg gatactctgg
    13441 cctaaatttg aggaccagga agttggactt aagttggctc tgacaggggc tatcaaggac
    13501 caactcttgc ccatggacga agtcaccatt tttggtttgg actacttcaa agccaactac
    13561 ccagaacgtc tacaagagcg gttcaagggc atggatttgg agcaggaaac acctgaactt
    13621 ataatggact ggaccaagaa attgggcttc cgtgatgatt atgaccgttt ttacaatctc
    13681 tttgtcaagg acgttcgaga tggtcgttta ggcacttata cattagacag ggtgagtgac
    13741 ttagatggca acgattaagg aagtgacagc tctgctggcc caggtagaca gtctggacag
    13801 tccagtctgg gaccaactgg ctcaggatga gcgagcaggg gttcaggctg ccatcaagaa
    13861 acgccaaaag gagttggaaa aagaagcagc agaagatgct cgcttagaag ctatgctatt
    13921 ctacgaaaaa gccctttatg acaatggggt ggagttcatc gctggtatcg acgaagtcgg
    13981 ccgggggcct ctagcaggtc ctgtggtggc agcggcggtc atcttgccca aagggtgtaa
    14041 aatccgctac ctcaacgact ccaagaaaat ccctaagtcc aagcacgaag caatctacca
    14101 agaagtcatg gagcgagcgg tggcagtcgg tgtaggcgtc aaagatgcag ctgtcatcga
    14161 tcaggtcaac atctacgagg cgaccaagtt agccatgctg gaggctttgg ggcagttgag
    14221 ccaagagcct gaacaccttt tgatagatgc tatgaagttg gatacacccc taccgcaaac
    14281 ttctatcatc aagggagatg ccaatagcct gtctatcgca gctgcctcta tcgtggccaa
    14341 ggtgacgcgg gataagatga tggcagacta taacaaggag ttctcaggct acggcttcgc
    14401 taagaatgct ggttatggaa cggcagagca tttggaaggc ttgaacaagt taggcatcac
    14461 gcccattcac cgtaagacct ttgagcccat taagtctatg gtagcagggg gaaactagtc
    14521 ttgacagtct ttttctggtt atttttagtg gctatcggtc tgtttgctct cgtctatttc
    14581 tgggagcggc ggtcgatttt tatctcactg tttgctttga acatcctggt ctgggccttg
    14641 gcatggatta tctcaacagg cattctcgaa aacaatgaaa tcttgcgctt gctggctata
    14701 gcagtggcta tggtgctctt ttttgccttg ttaagtggtc catttgtctt gcttttcaca
    14761 ctttatctaa atggttttca gattttgaag agagaagggg ttcgcttcca taatttccta
    14821 tccatgggac tggcggtagc cttgactttt tatctcttca tcgctccctt tgtggttcaa
    14881 tctctgtctg gtattagttt gttaaacatg gtgtttgtct atgttggatt tttagtgtct
    14941 tatgccatta tcatcagcat gctctatacc acgtctagct ttgtcaattt ggtcaatttc
    15001 ttcccaggca agcttgatta tgtagtggtc ttaggtgctg gcttgattgg tgacaaggta
    15061 acgccacttt tggcttcgcg gattgaaaaa ggcatagcag tctaccggaa acatcctggc
    15121 agtaagctca ttatgtcagg cggacaaggt cctgatgaaa ttatagctga aggtcaagct
    15181 atggcaaatt atgccttgga acaaggtatt cctgtagaag atattgtgat agaaaaccaa
    15241 tcaaccaata ccgaggaaaa cttgaaattt tcctatgccc tgatgaaacc tggcagtcga
    15301 tttgcccttg taaccaatta ctatcacgtt ttcagagctc tgctattggc acgaaaactg
    15361 aaaatcaaat gtataggcta tggagcaaaa accagatttt actttagcct caatgccttt
    15421 attcgggaat ttgtaggcta tgtagtcatg agccgaaagg tgcatttggt ctttttgagc
    15481 atcgtgtcag ccctttacct gatcggagtg attatcagtt taatgcatta aaaacagcct
    15541 ctaggtgact agaagctgtt ttcttataac ataaagtaga caatggcgat atactgaagt
    15601 gccgaagcta gtaggataaa gagatgccaa atcatgtgga agtagggctt tttcttggcg
    15661 tagaagaggg ctccaatagt ataagataat ccaccagcca gcatgagaat gccaaatgcc
    15721 attccagtct tttgcaaaat ggctggcaag ataaagacaa ccaaccagcc catgaaaaga
    15781 tagaggaaga ggctgaattt ttcgtttatt tttttagcaa aaattttata gagtatacca
    15841 aaaatagtca atccccattg caaacctaca atcagataac ctagccaacc accgaccaag
    15901 gatagggcaa caggtgtgta gcttccagcg atagcaatgt aaatcatgct atggtcgata
    15961 atcctcagga tgtatttgtg agtagaagca tagggcatgg agtggtagat ggaagaggat
    16021 aggaacatga gaaataggct gatgacaaag atggacatac caatcacagc ggtcaggtta
    16081 taatcttgat aggaatgaat agcagtaacg gggagtaaga ccaacataag cagggctccg
    16141 actgcatgtg tgacactgtt agcaacctcc tctccaaagg atagttgcag gctcaatttc
    16201 aaactaggat tcatggtgac tcctttctaa ctccatagtg agttgacggg ttttgtaata
    16261 tagtttaaca gactgacagg cataacgaat gatttcaggc gtttcgtcgc tctcctcatt
    16321 tcgcatgcag tcaacaaaat gattgtataa ggtttctgaa ttgctagact ggagaagaag
    16381 atttaaggtc tgactgattt cttgaatcga aaagacattt tttaagatag tcaaggcaat
    16441 caagcgggca acttgctgtt tttggtattt tttctttatc ggtttttcaa gttgcttgtg
    16501 cttgacataa ttgttaatca tagcagcagt caatactctt cgaaaatcaa tattatccgt
    16561 tgtcaccttg ccttgatgaa ccccagttct atcatcggtt tcatttccta ggctactgtt
    16621 gattttcatt gagtataaac tcttctgttc aaggctttcc tgagaattga ctacttgatt
    16681 aacatacagc agaacttggt ccaaatacaa atctaaatca ggtaattcat gccagaaggg
    16741 gatagtttgt ttcatgatta tctactctct tttatctagt aatgataact agattgtacg
    16801 atatacaata tttaatgtca agaaaaaacc ttctggaccc aaaatcacag aaggttagtt
    16861 gcttatggtt ggataatatt ttcaggcaat gtcatcttat aaagtgaata ggctccatct
    16921 aggtttttaa
//
LOCUS       DATUCG010000045        16648 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.046, whole genome shotgun sequence.
ACCESSION   DATUCG010000045 DATUCG010000000
VERSION     DATUCG010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16648
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.046"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(23..742)
                     /locus_tag="VCA20_001137"
     CDS             complement(23..742)
                     /locus_tag="VCA20_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEP1834974.1"
                     /translation="MKTVKEVSQLSGISVRTLHYYDEINLLTPSFIAENGYRYYDNEA
                     FEKLQEILLFRELEFPLKEIKKIVGNAAYDREAALKDQIRLLELKKKHLEKVIKHAKS
                     LQQKGENYMNFEVFDKSDLLAFQEEAKERWGNTAAFQEFSAKTNQGDFAQISSEMSGI
                     MMEFGQLKKLSADDSKVQKQVEVLKAYISENFYNCTNEILASLGQMYIADNRFTQFID
                     QVGGEGIATFVSQAIAIYCGK"
     gene            complement(803..1243)
                     /locus_tag="VCA20_001138"
     CDS             complement(803..1243)
                     /locus_tag="VCA20_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1834975.1"
                     /translation="MTVWFQDVVDSLQMISQGDRYYYDSHIDELVYLSVGEFRIESRD
                     GLEQEIEEDVAGRFVGLPTYYDFNPYTFMELYISDLTDGDLSGRLSRAIRGRGAFRRF
                     KNELERCDRLEEWYAFETQCYKELVLEWCQENKIAIVDNQLKKM"
     gene            complement(1247..2900)
                     /locus_tag="VCA20_001139"
                     /pseudo
     CDS             complement(1247..2900)
                     /locus_tag="VCA20_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735479.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2999..4087)
                     /locus_tag="VCA20_001140"
     CDS             complement(2999..4087)
                     /locus_tag="VCA20_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEP1834976.1"
                     /translation="MISGIIAEYNPFHTGHKYLLEQAEGLKIVVMSGNFMQRGEPAIV
                     DKWTRTQMALEHGADLVVEMPFLVSVQSADHFAKGAISILHRLGVEKLVFGTEEMLDY
                     QKIADIYVDKSKEMENFVKNLPDHLSYPQKTQAMWQEFAGLTFTGDTPNHILALAYAK
                     AVAGTGIQVGPVQRQGAGFHSEEVETTYASATAIRKGADQLDLVRGFLPSASLFEEAT
                     KVSWNDYFPLLRYQIATHRALSQVFQVNDELASRIRSAIGSVATVEELVDAVATKRYT
                     KARVRRVLTYILVNAVETPLPEAVHVLGFSTRGQAYLKQVKERVGLVTRIGKEPWDSL
                     TQQADVVYQLGADAMAEQTYGRVPVRVE"
     gene            complement(4084..4830)
                     /locus_tag="VCA20_001141"
     CDS             complement(4084..4830)
                     /locus_tag="VCA20_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEP1834977.1"
                     /translation="MATYETFAAVYDEIMDDSLYDKWTDFSLRNLPQDTKTILELACG
                     TGIQAVRFAQKGYEVTGLDLSYEMLELAQKKAEAAGVMMELGQADMMALEGVGDFDAV
                     TCYSDSLCYMSDREAVLRVFEGVHSVLNQGGTFLFDVHSIYQMEEVFSGYSYHENYED
                     FAFVWDTYEGEHEYSIVHELTFFVKDEDSQEERFIRRDEVHEERTYPIETYVELLKEA
                     GFASVEVFADFEDQAPTETSQRWFFKAVKA"
     gene            complement(4985..5344)
                     /gene="rsfS"
                     /locus_tag="VCA20_001142"
     CDS             complement(4985..5344)
                     /gene="rsfS"
                     /locus_tag="VCA20_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEP1834978.1"
                     /translation="MKELDLVKVVVQAADDKRAEDLVVIDVQGVTSLTDYFVIASSMN
                     SRQLEAIAENIREKVAEVGIKSSRVEGDSNGGWVLLDLGGVVVHIFSEEMRDLYNLEK
                     LWHEGSFLEVADFLEEE"
     gene            complement(5346..5855)
                     /locus_tag="VCA20_001143"
     CDS             complement(5346..5855)
                     /locus_tag="VCA20_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEP1834979.1"
                     /translation="MKSALLVIDIQNLLVVEKPYAIEERLALWQDSITKARQAGLEII
                     HVRHHDQELVKGTVDWEIHSIVAPLASEKIFDKTFNSAFKETGLHAYLQEKGIEQLII
                     MGMATNFCVDTTIKVPFELSYKVAVIRDGTTTSYSGKLDVKDLIDHYQNTWSWNFAQV
                     DRLENIIRG"
     gene            complement(5852..6297)
                     /locus_tag="VCA20_001144"
                     /pseudo
     CDS             complement(5852..6297)
                     /locus_tag="VCA20_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735474.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(6300..6887)
                     /gene="yqeK"
                     /locus_tag="VCA20_001145"
     CDS             complement(6300..6887)
                     /gene="yqeK"
                     /locus_tag="VCA20_001145"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEP1834980.1"
                     /translation="MITADLTFDRQALLEKIRAAMKPARFQHVLGVEQAALALADQYG
                     CDPKKASLAALLHDYAKEVEDQVFLDLIAKYDLDRDLLNWDNNIWHGVVGAYKIAEDF
                     GLEDQEIFQAIQRHTIGAGQMTLLDKVLYVADYIEPNRDFPGVDEARRIAKESLDKAV
                     AYETAQTISYLAKKGIPIYPQTLETYNGYVSYLKE"
     gene            complement(6884..7516)
                     /locus_tag="VCA20_001146"
     CDS             complement(6884..7516)
                     /locus_tag="VCA20_001146"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEP1834981.1"
                     /translation="MAIELLTPFTKVELEVEKKETNRKQVGILGGNFNPVHNAHLIVA
                     DQVRQQLKLDQVLLMPEFIPPHVDKKETIDEYHRYSMLKMAIAGMEGLGIETIELERR
                     GVSYTYDTMKLLKEKNPDTDYYFIIGADMVDYLPKWHRIDELVQLVQFVGVQRPRYKA
                     GTSYPVIWVDVPLMDISSSMVRSFIKQGRVPNFMVPHEVLDYIEEKGLYQ"
     gene            complement(7529..7840)
                     /gene="yhbY"
                     /locus_tag="VCA20_001147"
     CDS             complement(7529..7840)
                     /gene="yhbY"
                     /locus_tag="VCA20_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEP1834982.1"
                     /translation="MMLTSKQRAFLNSQAHSLKPIIQIGKNGLNDQIKTSVRQALDAR
                     ELIKVTLLQNTDENIHEVAEILEEEIGVDTVQKIGRILILFKQSSKKENREISKQVKE
                     I"
     gene            complement(8108..9214)
                     /gene="yqeH"
                     /locus_tag="VCA20_001148"
     CDS             complement(8108..9214)
                     /gene="yqeH"
                     /locus_tag="VCA20_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEP1834983.1"
                     /translation="MEELFCIGCGAQIQTLDKAVAGFTPQSALEKGLETGQLYCQRCF
                     RLRHYNEISDVNISDDDFLKLLHSVGESDALVVNVIDIFDFNGSVIPGLPRFISGNDV
                     LLVGNKQDILPKSVKTGKVTQWLTERAHEIGMRPVDVVLTSAQNKQAIKDLIEKIEQH
                     RKGRDVYVVGVTNVGKSTLINAIIQEITGDKDVITTSRFPGTTLDKIEIPLDDGSYIY
                     DTPGIIHRHQMAHYLTAKKLKYISPRKEIKPKTYQLNPEQTLFLAGLGRFDFVAGERQ
                     GFTAFFDNELQLHRTKLQGASEFYQKHAGSLLVPPTSKELKEFPELARHEFTINEKTD
                     VVFSGLGWIRVNEKAKIAAWAPKGVDVVIRKAII"
     gene            complement(9215..9742)
                     /locus_tag="VCA20_001149"
     CDS             complement(9215..9742)
                     /locus_tag="VCA20_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEP1834984.1"
                     /translation="MSLENYMPDFALEKAYDVTVESLKKHGIKVVFVDLDNTLIAWNN
                     PDGTSEMRQWLHDLQDAGIPVVVVSNNKYERVKRAVEKFGIEFEAFALKPFTFGINRA
                     LKRFDVQPYEVIMIGDQLMTDIRAAKRAGLKSVLVKPLLKTDSINTQINRWRERRTMK
                     KIIAKYGAIDYKREM"
     gene            complement(9785..10150)
                     /locus_tag="VCA20_001150"
                     /pseudo
     CDS             complement(9785..10150)
                     /locus_tag="VCA20_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935151.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10357..10986)
                     /locus_tag="VCA20_001151"
     CDS             complement(10357..10986)
                     /locus_tag="VCA20_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEP1834985.1"
                     /translation="MIKEFCSENHVGVMKALSLGAKRIELCDNLAVGGTTPSYGVVKH
                     VCQLAHENEATVMTMIRPRGGDFCYDEAEVEMMVEDCKVAIELGSDGLVYGLLTEENW
                     LDEPALERLFAVSQDCQIVFHMAFDQIPRERQFEALDWLAEHGVTRILTRGSLTGSAL
                     NNVEWLQEIVTYAQGKIEILIGGGLTVENVSTLLEPLPINQVHGTRLFW"
     gene            complement(11034..11942)
                     /locus_tag="VCA20_001152"
     CDS             complement(11034..11942)
                     /locus_tag="VCA20_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEP1834986.1"
                     /translation="MAEKRLGTLITLIAGIAWGLSGVSGQYLMSRGVSVDMITSLRLL
                     VSGFFLVGLAYATAKEQLLIVLKNRKALLGIFVFAMLGLVLNQMAYLQAIYHTNAGTA
                     TVLQYLCPILVLAYTCLKNGEKPSGIELISIILAVAGTFLIATHGKLDELAVTPIGLF
                     WGIFSAFTYALYIILPGKLIRQYGSITVIGLGMLMGGFVVTLGLQTWQHHLPIDGGSF
                     LGLLGIVGVGTIFAYTAFLKGVSMVGPVNGSLLASIEPIASVFFAVWLVNEQFYAIDF
                     VGMLLILLAVLLISLKDILVAQKSIG"
     gene            complement(12174..13655)
                     /gene="galT"
                     /locus_tag="VCA20_001153"
     CDS             complement(12174..13655)
                     /gene="galT"
                     /locus_tag="VCA20_001153"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEP1834987.1"
                     /translation="MMTGLVDRFVEQVIANSDFEEMDALYLRNRVLALVGDQVLTVRT
                     ELTDLIELKEELLTHGVRTGFVGELLEEQDMVGACLMDLMTPSPSQVNRDFWQTYQDS
                     PEQAIGDFYELSKRNDYIKMAAIAKNIYYPVSTEYGDLEITINLSKPEKDPKSIVAAT
                     KAEASNYPKCLLCMENEGYQGRINHPARANHRIIRLDLGQEQWGFQYSPYAYYNEHAI
                     FLNQEHVPMVISPRTFEQLLDLLDLLPGYFVGSNSDLPISGGSILTHNHYQGGRHSFA
                     MEKAPIERQLVFDGFESVSAGIVKWPMSVIRLSSADKPSLLGLATKILEKWRSYSDDN
                     VQIKAETDGTPHHTITPIARKRGELYELDLVLRDNQTSEEFPDGIYHPHPDVQHIKKE
                     NIGLIEVMGLAILPPRLKAELAEVEKFLLGQDSQVADYHQPWAESLKTAHPDVTEETV
                     EQVVRESVGQIFARVLEDAGVYKRTPEGQAAFLRFVEFVGLAT"
     gene            complement(13665..14837)
                     /locus_tag="VCA20_001154"
     CDS             complement(13665..14837)
                     /locus_tag="VCA20_001154"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEP1834988.1"
                     /translation="MNTEQLKQAFLDVFGQEADASFFSPGRINLIGEHTDYNGGHVFP
                     AAITLGTYGAARKRDDQVLRFYSANFEEVGIIEVDLNHLVFDKADNWTNYAKGVLKFL
                     QEAGHVIDTGMEVFVYGNIPNGSGLSSSASLELLIGIIAEELYGLELTRLDLVKIGKQ
                     TENHFIGVNSGIMDQFAIGMGADQRAIYLDTNSLEYELVPLDLGDHVIVIMNTNKRRE
                     LADSKYNERRAECEKAVDELNAVLTIQTLGELDEWTFDQYSYLIKDENRIKRARHAVL
                     ENQRTLQARKALEEGDLATFGRLVNASHVSLEHDYEVTGLELDTLAHTAWEQEGVLGA
                     RMTGAGFGGCGIAIVHKDKVEAFKENVGKTYTEVVGYEPSFYVAEIAGGSRVLSRK"
     gene            14970..15968
                     /locus_tag="VCA20_001155"
     CDS             14970..15968
                     /locus_tag="VCA20_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEP1834989.1"
                     /translation="MVTIKEIAEKAQLSSATISRVLKNDLTLSVSQETRDRIFNLAQE
                     LGYTKHLKKQAPQQKGTIGIVQWYTESEELADLYYYSIRASIEQTASLLGYQIVRSFN
                     DLTNPLLQGLGGIIAVGKFSSGQIAELTNLSSKLIFVDSDTLTEGFSCVTTDFEHSVQ
                     TVIDHFRSQGLTDIGLLVGQEETTDGHQLPTDPRLTAFRDYLDTLCMLQEDYIYQGKF
                     STQSGYELMSQAISDLGDQLPPAFFMANDTLAVGALRALQERQIAVPDRVQLITFNDT
                     AITRQVYPALSSISVFTEEMGQEAMQLLDRMIASPQPHHPRKIKLGTQLVIRESSY"
     gene            16145..16318
                     /locus_tag="VCA20_001156"
                     /pseudo
     CDS             16145..16318
                     /locus_tag="VCA20_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429573.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
BASE COUNT         4728 a         4254 c         3071 g         4595 t
ORIGIN      
        1 gttttcacta gacttttctt atttattttc cacaataaat cgcaatcgct tggctgacaa
       61 aggttgctat accttctcct cccacttgat caataaattg agtaaaccgg ttatctgcaa
      121 tatacatttg tcctagactt gctagaattt cattggtaca attataaaaa ttctcactaa
      181 tataagcttt aagaacctct acctgtttct gaactttaga atcatcagct gataattttt
      241 tcaattgtcc aaattccatc ataataccag acatttctga acttatctga gcaaaatcac
      301 cctgatttgt cttagcagaa aattcctgaa aagctgcggt attaccccat ctttctttag
      361 cttcctcctg gaaagccaac aaatctgact tatcaaacac ttcaaaattc atgtaatttt
      421 ctcccttctg ctgtaaagac ttggcatgct ttatgacttt ttccaagtgt ttctttttca
      481 actccagtaa tctaatctgg tctttcaaag ccgcttctct gtcatacgcc gcattcccta
      541 caattttttt aatttctttg agaggaaatt ccaattcacg aaaaagtaag atttcttgca
      601 acttttcaaa cgcctcatta tcataataac gatatccgtt ttccgcaata aaactaggtg
      661 tcagcaaatt tatttcatcg taataatgaa gtgtgcgcac actgatacct gaaagctgac
      721 tcacttcctt aactgttttc atggaaaatc tcctctctat gatcagtata agggtaacgt
      781 aaggtgagag tcaagggaaa tattacattt ttttcagctg gttgtccaca atagcaatct
      841 tattttcctg gcaccattcc agtacaagtt ctttgtaaca ttgtgtttca aaggcatacc
      901 attcttccaa acggtcacat ctctctaact cattcttaaa acgtctaaaa gcaccgcgcc
      961 ctcgaatagc tcgagataga cggccagaca agtctccatc agtcaaatca gaaatataga
     1021 gttccataaa tgtgtaaggg ttgaagtcat aataagtcgg tagacctaca aaccttccag
     1081 ccacatcctc ctcaatctcc tgttctaaac catcacggga ttcaattctg aactccccaa
     1141 cagacagata gaccaattcg tctatgtgac tatcataata gtacctatca ccttgactaa
     1201 tcatctgcaa cgaatcaacc acatcttgaa accaaacagt cataccctac cacctcactg
     1261 catctaattc ctctatcatg gctttgcgtc gtggatattg cactttccac gacgtgcgaa
     1321 gacttgtact caccactttt ccatcaggta aacctgcaat ttttaccaaa taccctgcca
     1381 actgacgata gcggcttcga ctactagcca catccatctg ctgacagaga gcttgagcgt
     1441 aggcatctcg taattgctca ggcagtttct ctgataaata gtcttcaaat cgatccaaat
     1501 tttctaaggc ccagtaactt gctgtaatgc ggtccaataa ttcctgcggt cggtctgcca
     1561 attgcaacaa tttcaactgc tgtgaccgga aacgaccatc ctccagcaaa tcatctaagt
     1621 agctctgcca ttcttcccta gaaacatagt cccgtaactc ctctaccagc tccaaatcag
     1681 tatcaccagc agagaaaatc atctccagca attcttctct gaccttgtca tcctgcccct
     1741 gagaccggta gatgtcccgt aactgctggc tataatccga caccagtccc ctatactggg
     1801 catccaggcg cttactttcc tgcaagaggt gaactgcacg gtctagatgc tgatttgcta
     1861 cagcctgggc aatggctagt ttacgaagtc cagccacctc ccaatgattg gcctggaagg
     1921 tcaacaagtc tgcctcacta gagtccaact gaaccagcaa cctgtaacag agcaaggcaa
     1981 atcggtcacg acggaatttc catgaccaag aggactgggt ctccttcttc tatcgcctgc
     2041 aaaacagctt gaaaataggt caatttctgc tcaaggaaat gttcttcttg aaactctgtt
     2101 tctacaaatt ccatgagaag gtcttctgca acctcatagc cacttagctt ttctaagcac
     2161 cagtctagta attcttggac aatcttatct ggagctacag tcaataaatt ctgccagtag
     2221 ccagcaagta tataaagcag actaccagtc gttccattgg aatcatccaa ctcttgctga
     2281 caaatctgat gatagaaggc atctgataca gccaagaaat tcgcatactc acacctatcc
     2341 agcaaaggct ggagatactg atgcatggcc ttttccacgg cccgttcgta atccctagtc
     2401 tgcctatact cgataaagcc cgtacgcctg tcctcaaaag gcaaacccaa gtctctgatt
     2461 tcctttttca catcttccac ttgctgaggt gttagctggg atgagaaacg cagttgtagc
     2521 cgattggcta gttccctatc ttcttgggct aattcaagaa caacagcccg tagttgctca
     2581 actgtcagtt tatccaggag ctgagcaagt gacacatctt tgccctcaag agcgggctgg
     2641 ctatctaatt cagaaatagc aaacaagact gctgccatat gcttgcagtg gttcatttct
     2701 tgagcatggg gacactcaca ccacatattg accaactgac cgtgctcaaa gctaatctcc
     2761 acctgataga cttgactacc agagacaagg gcatagtaac tctcacccac ctcttcaagc
     2821 ccttgaacag catcgtctaa aaataaactg caacccctat ctacaatgtg aggcttaaaa
     2881 tagtccatcc aatttttcat aatttatttc ctttgatttt tctacttaaa ttatacaaaa
     2941 aaaatatgaa ggttttcaag tcaacgacaa aaacttcctt ttttctctag atttcatctc
     3001 actccaccct caccggcaca cgcccgtagg tctgctctgc cattgcgtca gcgcccagtt
     3061 gatagaccac atccgcctgc tgggtcaagc tgtcccaggg ctcttttccg atccgcgtca
     3121 ctaaccccac tcgctccttg acctgcttga gataggcctg gcctcgagtt gaaaagccta
     3181 atacatggac cgcttctggc aagggagttt ccaccgcatt gaccaagatg taggtcagca
     3241 cccgccgcac ccgcgccttt gtgtagcgct tggtcgcaac ggcatccact aattcctcca
     3301 cggtcgctac gctcccaatg gcagagcgga tacgactggc cagctcatcg ttgacctgaa
     3361 agacctgact gagagctcgg tgggtcgcaa tctggtagcg gagcagggga aaataatcat
     3421 tccagcttac cttggtcgcc tcttcaaaga gactggcaga cggtaaaaag ccacgcacca
     3481 agtccaactg gtcagcaccc tttcggatag ccgtcgctga ggcgtaggtc gtttccacct
     3541 cttccgagtg aaaaccagcc ccctgccgct gaactggacc gacctgaatg cctgtcccag
     3601 ccaccgcctt agcataagcc agagccagaa tatgatttgg cgtatcacct gtgaaagtca
     3661 gcccagcaaa ctcctgccac atagcctggg ttttctgtgg ataagagaga tggtcaggca
     3721 agttttttac aaaattttcc atctctttcg acttatccac atagatatcc gcaattttct
     3781 ggtaatccag catttcttcc gtaccgaaaa ccagtttttc cacacctagc ctgtgtaaaa
     3841 tgctgattgc tcccttggcg aaatggtcag ccgactggac cgacaccaaa aagggcatct
     3901 cgacaacaag atctgccccg tgttccaaag ccatctgagt ccgtgtccac ttatccacaa
     3961 tagctggctc accccgttgc ataaaattgc cagacataac tacaattttt agcccctcag
     4021 cctgctccag caggtattta tgacctgtat ggaagggatt gtattctgct attatccctg
     4081 aaatcatgcc ttgaccgcct tgaaaaacca ccgctggctt gtttctgtcg gcgcttgatc
     4141 ttcaaagtcc gcaaagactt ccacagatgc aaaaccagct tctttcaata attccacata
     4201 ggtttcaatc ggataggtcc gctcttcgtg aacctcatcc cgacggataa acctctcctc
     4261 ttgactgtcc tcatctttga caaagaaggt caattcatga acaatggaat actcatgctc
     4321 accctcatag gtatcccaga caaaggcaaa atcctcgtag ttttcatggt agctatagcc
     4381 tgaaaatact tcctccattt gatagatgga gtgaacgtca aaaaggaaag taccaccttg
     4441 attgagaaca gagtgaactc cttcaaagac ccgcaaaacg gcttctcggt ccgacatata
     4501 gcacagactg tctgagtaac aggtcaccgc atcgaaatct ccaacacctt ccagagccat
     4561 catatctgct tggcccagtt ccatcatgac accagcagct tctgcttttt tctgggccaa
     4621 ttccaacatt tcataggaca ggtcaagccc tgtcacctca taaccctttt gagcaaaacg
     4681 cacagcctga atccctgtcc cacaggccaa ttctaaaatt gttttggtgt cttgtggcaa
     4741 gttccgcaag ctaaaatctg tccatttatc atacaagctg tcatccatga tttcatcata
     4801 gaccgccgca aaggtttcat aagttgccat attttccctt tcataaaaag cagcccatcg
     4861 actgcttcat taactcacac tgtgttcaag aagttcatcg cttctaatta cataatagtg
     4921 aactctcttt ggtcactttc gagttttaag gtgaccagtt gaaacagttc tccaaactgt
     4981 ttcactattc ctcttctaaa aagtctgcga cttccaagaa gctgccttcg tgccataatt
     5041 tttcaaggtt gtagaggtca cgcatttctt ctgagaagat atgaacgact acgcctccca
     5101 ggtccaaaag gacccagcca ccgttactat cgccttctac acggctactc ttgattccaa
     5161 cttccgctac cttttcacga atgttttccg caatagcctc caactgccga ctgttcatgg
     5221 agctggcaat cacaaagtag tctgtcaggc tggtcacgcc ttgaacatca attacaacaa
     5281 ggtcctcagc tcgcttgtca tcagcagcct gaaccacaac ttttactaaa tctagttctt
     5341 tcatcttatc ctctaattat attttctaat ctatccacct gggcaaaatt ccatgaccag
     5401 gtgttttggt agtggtcaat caagtctttg acatccaatt tgcctgaata acttgtcgtc
     5461 gttccgtccc gaatcacagc taccttgtag cttagttcaa agggtacttt aatcgtcgta
     5521 tcaacacaaa aattggtcgc catacccatg atgatgagct gctcaattcc cttttcctgc
     5581 aaataagcat gaagccccgt ttccttgaag gcactgttaa aggtcttatc gaaaatcttc
     5641 tcactagcta agggtgcaac aattgagtga atctcccaat caacagtccc cttgaccaat
     5701 tcctgatcat gatggcggac atggatgatt tccagtcccg cttgacgggc ttttgtgatg
     5761 ctatcttgcc agagggctaa tcgctcttca atggcataag gtttttccac cactagaagg
     5821 ttttgaatgt caatgactaa caacgcagat ttcatagctt gattccaggc gtttccaaac
     5881 ggaagaccaa ttccctttct tcttcaagct ccagaacaaa gccttttttc tccaagagtc
     5941 ttgccatctt ttggttgccc ttaacatatg ctgcaatcaa atggtcgcag acaggatgtg
     6001 attgagccat tgctattacg acctccaaag cagcctggcc atagcccttc ccctgaaatt
     6061 cttgaccaat cataaaccgc cagagcatat actgctgtat gtcttcatca atatcaatta
     6121 agacaaaacc aaccatctgc ccagatagca taggaaaaca tccccgtttt ctctgtaaag
     6181 ccaagcgtca gcgagtgaac gcacgtttgg agccacgaac cccttatctg cctcggctac
     6241 tgtcaagtct aagacagctt gataggaaat ctcatccaca ggttttaata caatcatccc
     6301 tactccttca aataagaaac atagccatta taggtttcca aagtttgtgg ataaatggga
     6361 attcccttct tggctaagta agaaatggtt tgggcagttt cataggctac tgccttatcc
     6421 aaggactctt ttgcgatacg acgcgcctcg tccaccccgg gaaaatcccg attaggttcg
     6481 atgtagtccg ccacatagag caccttgtcc agcaaggtca tctgcccagc accaatggtg
     6541 tgtcgttgaa tggcttggaa gatttcttgg tcttccaaac caaaatcttc tgcgatttta
     6601 taggccccaa ccacaccgtg ccagatgtta ttatcccagt tgagcaggtc cctatccaaa
     6661 tcatacttgg caatcaaatc cagaaagact tggtcttcca cttccttggc atagtcatgc
     6721 aagagtgctg ccagactggc ttttttcgga tcacagccat attggtctgc caaggcaagc
     6781 gccgcctgct ccacacccaa gacatgttgg aagcgcgctg gcttcatggc cgcacggatt
     6841 ttttccaaaa gagcctgacg gtcaaatgtc aaatctgctg taatcattgg taaagtcctt
     6901 tttcttcgat atagtccaga acttcatgag gcaccataaa attcggcacc cgtccttgct
     6961 tgataaaact acgcaccatg ctggaggaaa tgtccatcaa gggtacatct acccagataa
     7021 caggatagga agtccctgcc ttgtaacgag ggcgctggac accaacaaac tgcaccaact
     7081 ggaccaattc atcaatcctg tgccattttg gcaggtagtc taccatatct gcaccgatga
     7141 taaaataata atccgtatca ggattttttt ccttcagcaa tttcatggtg tcgtaggtat
     7201 agctgacacc acggcgctcc agttcaatag tttcaatgcc caagccctcc atacctgcaa
     7261 tagccatctt gagcatggaa tagcgatggt attcgtcaat ggtttctttt ttatccacat
     7321 gaggcggaat gaattctggc atgagcaaga cttgatccag cttcaactgt tgacggacct
     7381 ggtctgccac aatcaaatga gcattgtgga cagggttgaa attcccaccc agaatcccca
     7441 cttgtttgcg attggtttct ttcttttcaa cctctaattc aaccttggta aagggtgtta
     7501 agagttcaat agccataggc tctccttctt agatttcttt gacctgttta gaaatttcac
     7561 ggttctcttt cttgctagat tgtttgaata aaatcaagat gcgaccgatt ttttggaccg
     7621 tatccacacc gatttcttct tccaaaattt ccgctacttc gtgtatattt tcatccgtat
     7681 tttgtagcaa ggtcaccttg atcaattcac gcgcatccag cgcctgacga acactggtct
     7741 taatctggtc attgagacca tttttcccaa tctgaataat gggtttgaga ctatgtgcct
     7801 gactgttcaa aaaggcacgt tgttttgaag ttagcataat ttattataca aagggcgttg
     7861 taacagactg ggagactgtt acaatcggga aataaagaaa acaaagtttt tccttattgc
     7921 gccgatatac ttagcccgtg ttcaaatcag aagacttgaa cttcctttct ctttaattgt
     7981 aaaaactgaa atcaaatacc tcttatactc aatgaaaatt aaaatcagac taggcgacgc
     8041 agatgcagat agaactggag ttcatcaaat caagtcaaca acatctgctt ttaattttcg
     8101 aagagtatta gataatggcc ttgcggatca ccacatccac acctttaggt gcccaggcag
     8161 caatcttggc tttttcattg acacgaatcc aaccaagtcc tgagaagacc acatccgtct
     8221 tctcgttgat ggtaaattca tggcgggcca attcaggaaa ttctttcaat tccttgctgg
     8281 ttggtggcac taaaagtgag cccgcatgtt tctggtaaaa ttcactagct ccctgcagtt
     8341 tggtacggtg gagctgcaat tcattgtcaa agaaggccgt aaagccttgg cgttcaccag
     8401 ctacaaaatc aaatcgtccc agaccagcca agaacaaggt ttgttctgga ttaagctgat
     8461 aggtctttgg cttgatttcc ttccgtgggc tgatatactt gagtttttta gccgtcaaat
     8521 aatgggccat ctggtgacgg tggataattc ctggcgtgtc atagatgtat gaaccatcat
     8581 ccagaggaat ttcaatcttg tccagcgtcg ttccagggaa acgtgaagtc gtaatgacat
     8641 ccttgtcccc tgtgatttct tgaataatag cgttaatcaa ggttgacttg ccgacgttgg
     8701 tcacgccgac cacatagaca tcccgtccct tgcggtgttg ttcaatcttt tcaatcaagt
     8761 ccttgatagc ctgcttgttt tgagcagaag tcaagaccac atcaactggt cgcatgccga
     8821 tttcatgtgc ccgctcggtc aaccactgag taaccttgcc tgtcttgaca gacttgggta
     8881 aaatgtcctg cttgttgccg accaaaagca cgtcatttcc agaaataaag cgaggcaagc
     8941 caggaatgac tgaaccattg aaatcaaaaa tatcaatgac attaaccacc agggcatcgc
     9001 tttctcctac gctatggagg agtttcaaga agtcatcatc tgagatattg acatctgaaa
     9061 tttcattata atggcgcaaa cggaaacagc gttggcagta gagttgccct gtttccaggc
     9121 ctttttctag ggctgattga ggtgtaaaac cagcgacagc cttgtctagc gtctgaatct
     9181 gggcaccaca gccgatacaa aataattctt ccacttacat ctccctcttg tagtctatcg
     9241 ctccatattt agcgatgatt tttttcatgg tccgtcgctc acgccaacgg ttaatctgcg
     9301 tattgataga gtccgtcttt agcaagggtt tgaccaagac agacttgaga ccagctcgct
     9361 tagcagcccg aatgtccgtc atcagctgat cgccaatcat aattacctca tacggctgga
     9421 catcaaagcg tttcaaagca cggttaatcc caaaggtgaa aggcttgaga gcgaaggctt
     9481 caaattcaat cccaaatttt tcaaccgccc gtttgacacg ttcgtattta ttgttagaca
     9541 ccaccacaac agggatgcct gcgtcctgca aatcatgtaa ccactggcgc atctctgacg
     9601 taccatcggg attattccaa gcaatcaagg tattatccaa gtcaacaaac actactttta
     9661 tgccatgttt tttcaagctt tcgacggtca cgtcataagc cttttccaag gcaaaatccg
     9721 gcatgtaatt ttctaaactc actgtcgtct ccaaagtttt ttatcaggta attataccat
     9781 ttttctaaga aaaatgcgaa tcgatagcca aaccgatttg ctctactcct gactcatcct
     9841 catccaaccc caaaatggta agagcatgtc cataatctgc atgactatca tcgaatagac
     9901 gccacgcgag agcgcgataa attaacttct tagtatcata gagactgtat ctctatctgt
     9961 actctgaaaa tgattgacaa tccttacctt ttggttcaac tcctttggtt ttagcaatgg
    10021 caatcaagct atcataatta tgataacgca aagtactatt caaggattgg gctaaacgaa
    10081 cagcatagtc caaagcaaaa tcactcacct caacagccac agttctcaat tctttacgta
    10141 ggggattcaa tagagacgca gcagtttcta gtctgtatac cggactttaa caaacaaaaa
    10201 tgaaaacagc cgaacatgtc gactgcttcc gtggagatta tatgaaaaag aataaatata
    10261 ttgtatcttc caatgcttaa acaaacctta acttctaaac atttctgaat aaacttcgat
    10321 tattactctg taatttattt cactttcaaa gctaccttac caaaataatc tcgttccatg
    10381 tacttgattg attggtaatg gttctaataa cgttgacaca ttttccacag tcaatcctcc
    10441 accaatcaag atttcaattt taccttgtgc ataggtgaca atttcctgta accattccac
    10501 attgttcaag gcggaccctg tcagacttcc acgcgtcaaa atccgtgtca ctccatgttc
    10561 tgccagccaa tcaagagcct caaattggcg ttcgcgagga atttggtcaa aagccatatg
    10621 aaagacaatc tgacagtcct gcgaaacagc aaacagtctt tccaaggctg gttcgtccaa
    10681 ccaattttcc tcggttaaca aaccataaac cagaccatct gaccccagtt caatcgctac
    10741 tttacaatct tcaaccatca tttccacctc tgcttcatcg tagcaaaaat ctccaccgcg
    10801 agggcgaatc atggtcatga ctgtggcttc attctcatgg gccagttgac aaacatgttt
    10861 gaccactcca taactcggcg tcgtccctcc gactgccaaa ttatcacaga gttcaatccg
    10921 tttagctcct agggataggg ccttcatcac gcctacatga ttttcagaac aaaattcttt
    10981 aatcatcttt attcctttca agttttttct ttagtataac aaaaaagagt atattaacca
    11041 atactctttt gggcaaccaa tatatctttt aaagaaataa gtaaaacagc aagtaaaatc
    11101 aacaacattc caacaaaatc aattgcataa aattgttcat tgaccaacca taccgcaaaa
    11161 aatactgagg caattggctc gatagaagct aagaggctac catttaccgg accaaccata
    11221 ctaacacctt tgagaaaggc agtataggca aaaatcgtcc cgactccgac aatacctagc
    11281 agacccagaa aacttccacc atcaatagga agatgatgct gccaagtttg caaaccaagt
    11341 gtgactacaa aaccacccat taacatccct aaaccaatca ccgttatact gccgtattga
    11401 cggatcaatt tacctggcaa aataatatat agcgcataag taaaggctga gaagattccc
    11461 cagaacaaac cgattggagt cactgccaat tcatctaatt tgccatgagt cgcaatcaaa
    11521 aaagtgcctg ctacggctaa gataattgaa atcaactcaa tccctgatgg tttttcccca
    11581 tttttcaaac aggtatacgc caagactaag atagggcaaa ggtattgtaa gaccgtagcc
    11641 gttcccgcat tggtatgata gatagcttgt aaataagcca tctggttaag aaccagaccc
    11701 aacatcgcaa aaacaaaaat tcccaacaag gcttttctat ttttcaacac tatcaacagc
    11761 tgttctttcg ctgtagcata agccaggcca acgagaaaga atcccgatac caacaaacgc
    11821 agggaggtaa tcatatccac tgaaacacca cgtgacatca agtactgtcc actcactcca
    11881 gacaagcccc aggctatacc tgctattaat gtaatcagtg ttcccaatct cttttccgcc
    11941 atcatatctc cttctcacac tgtgaatact ttcattatac caaaaagagt aggaatggag
    12001 gagaaaggca ggccgccctc cctaagttcg cgtcaacatc tcagcgcagt ggttgattgg
    12061 cagagttgtt cgcgtttcac gctccaaatc tgacctaatc aactgtgcgg ggggtgggaa
    12121 aacgaactct ttaaatcggt cgagttctgt cccactccca ctctttcttg caattatgtt
    12181 gctaaaccta caaattctac gaatcgtaga aaggctgcct gtccttctgg agtgcgtttg
    12241 tagacccctg catcttccag tacacgcgca aaaatttgtc ccactgactc acgaacaact
    12301 tgttcgacag tttcttccgt cacatctggg tgagctgttt tcaggctttc cgcccaaggc
    12361 tgatgatagt cagccacctg actatcttga cccagtaaga atttttctac ttcagccaat
    12421 tctgccttta agcgtggagg taaaatcgcc aagcccatta cctcaatcaa gccgatgttt
    12481 tctttcttta tatgttgcac atctggatgt ggatggtaga tgccatctgg aaactcttct
    12541 gaggtctgat tgtcgcggag aaccaaatcc agctcgtaca attctcctcg tttccgagca
    12601 atcggggtga tggtatgatg aggagttcca tccgtctcag cctttatctg aacgttatca
    12661 tctgagtagc tccgccattt ttccaaaatc ttagtcgcta aaccaagaag cgatggcttg
    12721 tctgccgaac tcaagcggat caccgacata ggccatttga caattcctgc tgaaacggac
    12781 tcaaagccgt cgaagaccag ctggcgctca atcggtgcct tttccatggc aaaactgtgc
    12841 cgaccacctt gatagtgatt gtgggtcaag atggagccac ccgagatagg gaggtcagag
    12901 ttggaaccga caaaatagcc tggcaacaaa tccaataagt ccaagagctg ctcgaatgtt
    12961 cgcggactga tgaccatcgg cacatgttcc tgatttagga aaatagcatg ttcattgtag
    13021 taagcatagg gcgaatactg gaagccccac tgttcctgtc ctaaatccaa gcgaataatg
    13081 cggtggttgg cgcgtgctgg atggttaatc cgaccctgat agccctcatt ttccatacag
    13141 agtaagcatt tgggataatt gcttgcttca gctttcgtag cagctacaat agacttggga
    13201 tccttctcag gtttggacag attgatcgtg atttccaaat ccccatactc tgtcgaaact
    13261 ggataataga tatttttcgc aatcgcagcc atcttgatat agtcattgcg cttgctcaat
    13321 tcatagaagt ctccaatcgc ttgctctgga ctgtcctgat aggtctgcca aaaatcacga
    13381 ttgacctgac tcggactcgg tgtcatcaaa tccatcaaac aagccccaac catgtcctgc
    13441 tcttccagca attcacccac aaaaccagtt cgaactccat gagtcaataa ttcttccttg
    13501 agttcaatca agtctgttag ctctgttcgg acagtcaaaa cttggtcccc aaccaaagcc
    13561 aagacccgat tgcgcaggta aagagcatcc atttcttcaa aatcactgtt ggcaatgacc
    13621 tgctcaacaa aacgatccac caatcctgtc atcatttctc caatctattt tcgagataag
    13681 acgcgagagc ctccagcaat ctccgctaca taaaagcttg gttcatagcc aaccacttca
    13741 gtataggtct tgccgacatt ttctttgaag gcttccacct tatccttgtg gacaatagca
    13801 ataccacagc caccgaaacc ggctcctgtc atccgagcac caagaactcc ttcttgctcc
    13861 caagctgtgt gagccaaggt atccaattcc aaacctgtca cttcataatc atgctccaaa
    13921 gaaacatggg aagcattgac cagacgacca aaggtagcca aatctccttc ttctaaagcc
    13981 ttacgagctt gcaaggtccg ttgattttcc aagacagcat ggcgggcacg tttgatacga
    14041 ttttcatctt tgattaaata actatattgg tcaaaggtcc actcatccaa ctctcccaga
    14101 gtttgaatag tcaagactgc attcaattca tcgactgctt tttcacattc tgcccggcgc
    14161 tcgttgtact tagaatccgc caattcacga cgtttattgg tgttcatgat gacaatcaca
    14221 tggtcaccca aatctagggg taccaactca tactccaagc tattggtatc caaataaatg
    14281 gcacgttggt ccgctcccat accgatcgca aactggtcca tgatcccaga attgacaccg
    14341 ataaagtgat tttccgtctg cttaccgatt ttcaccaaat caagtcgagt tagctcaagt
    14401 ccatacagtt cttctgcgat aatgccaatc aatagttcca aggaagctga tgatgacaag
    14461 cctgatccgt ttggaatatt gccatagaca aagacttcca tccctgtatc aataacatgc
    14521 cctgcttctt gcaagaattt gagaacacct ttggcataat tggtccagtt gtccgcctta
    14581 tcaaagacta gatgattcaa atctacctca ataataccta cttcctcgaa gttagcagaa
    14641 tagaaacgca aaacttggtc atcacgtttg cgagcagcac cgtaagtccc caaggtaatg
    14701 gccgcaggaa aaacatgacc accgttgtag tccgtatgct caccaatcaa attaatccga
    14761 cctggtgaga agaaagacgc atctgcctct tgaccaaaca catcgagaaa ggcctgcttg
    14821 agttgttctg tgttcataag aacctcctag attttgtaat cgttttcttt tattgtacaa
    14881 ctcaacaatt taaaagtcaa tatttttact aaatttttac taattttgtt ttttatgcta
    14941 tactagagac aaataaacag gagaatgcca tggttaccat taaagaaatc gctgaaaagg
    15001 ctcaattatc atctgccacc atttcccgcg tactcaagaa cgacctgacc ctatccgtca
    15061 gccaagaaac ccgcgaccgc attttcaact tggcacagga attgggctac accaaacacc
    15121 tcaaaaaaca agctccccaa caaaaaggca ccatcggcat tgtccaatgg tacacggaga
    15181 gcgaagagct ggctgacctc tactattatt ccatccgggc cagcatcgag cagaccgcca
    15241 gtcttctggg ctatcaaatc gtccgctcct tcaacgacct gaccaacccc ctccttcaag
    15301 gattgggcgg catcatcgca gttggtaaat tttcttctgg gcaaatagca gagctgacca
    15361 acttgtcttc caagctgatc tttgtggatt ctgatacgct gaccgaggga ttctcctgtg
    15421 tcacgactga ctttgaacac tctgttcaga cagttatcga ccacttccgt tcacaaggtc
    15481 tgactgatat tggactccta gtcggacagg aagaaaccac agacggacac caactgccaa
    15541 cagacccccg cctgactgcc tttcgtgact acctagacac cttgtgcatg ctccaagaag
    15601 actacatcta tcaaggaaaa ttctccaccc agtcgggcta tgagctgatg agccaggcaa
    15661 tttccgacct gggcgaccaa ctgcccccag ccttcttcat ggcaaatgat accctggcag
    15721 tcggcgctct gcgtgcttta caagagcgtc aaatcgcagt gcccgataga gtccaactca
    15781 tcacctttaa cgataccgcc atcacgcgcc aagtctatcc tgccctgtcc tccatcagcg
    15841 tcttcaccga agaaatggga caagaagcca tgcaactact agaccgtatg attgctagtc
    15901 cgcagcccca ccatccccgt aagattaagc tagggaccca gctggttata cgggagagtt
    15961 cttattgaaa gtgcataaga aaaaccgaga gttctgacgt atttagttca tggagttcgc
    16021 tagattccaa ccacctatca taaatgctgc accaaaaata aatacattta gcattttatc
    16081 ttcctctctc taatataatt ttgcaaccca attataacat acgttatatt tgttatcaac
    16141 cgtacatggt tacaatcaat tccgctggaa taagactgaa gctatcgaac tcgctaaaaa
    16201 tatgttaaat tacttggaaa gtgctaaatt tattgaccaa aattattatt cgaatttcat
    16261 caaagccttt atctacaact gtcataaatt aaataaaaca ggcgaagacc tattttaacg
    16321 ttaaagtaag ttctagtctg gcacaaaaac tagaaatcag actaatacaa aaaataaggc
    16381 agaacctact acgaaagctt gacataaact aggtaaagta agctaatcac taaaattaac
    16441 agattatttg tattttataa gtaatctgat ataatagaga tagaaaaggg gttggtctgc
    16501 aaaccaaccc catgtagaac cgtttaagac ggtgaccata tattagacta taactcgtcc
    16561 aacaactcga tcaaaagtgg ggacgggtta tttttttatc accatctttg aagattttat
    16621 agcacaagcc aatcaatgca atagtata
//
LOCUS       DATUCG010000046        16607 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.047, whole genome shotgun sequence.
ACCESSION   DATUCG010000046 DATUCG010000000
VERSION     DATUCG010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16607
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.047"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            210..756
                     /locus_tag="VCA20_001157"
                     /pseudo
     CDS             210..756
                     /locus_tag="VCA20_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941711.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
     gene            complement(795..1499)
                     /locus_tag="VCA20_001158"
     CDS             complement(795..1499)
                     /locus_tag="VCA20_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEP1834990.1"
                     /translation="MKIHVVNNQVEGATVALDILREKMNGGAKVLGLATGSSPLEFYK
                     LIRESDLDFSNVTSVNLDEYVGLGEESDQSYIHFMKENLFNAKPFKQSYLPNGSATDV
                     VAETERYNKILAEHPVDFQILGIGRNGHIGFNEPGAPFDGQTHLVELAPSTIEANARF
                     FDNPEDVPKQAISMGIANIMAAKTIVLMAYGPEKADAIKATVEGAVTEDVPASVLQNH
                     DNVILILDQAAASKLA"
     gene            1847..2875
                     /gene="queA"
                     /locus_tag="VCA20_001159"
     CDS             1847..2875
                     /gene="queA"
                     /locus_tag="VCA20_001159"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEP1834991.1"
                     /translation="MNTADFDFDLPEELIAQVPLEKRDSSKLLILDREKRSMVDSHFD
                     HIIDQLNPGDALVMNNTRVLPARLYGYKPETMGHVELLLLKNTQGDQWEVLAKPAKRL
                     KVGTTVAFGDGRLTATIVEELEHGGRIVEFSYEGIFLEVLESLGEMPLPPYIHKKLED
                     RERYQTVYAKENGSAAAPTAGLHFTQELLEKIEAKGVKLVYLTLHVGLGTFRPVSVDN
                     VDEHEMHSEFYNLSAEAAQTLNQVKDSGGRIVAVGTTSIRTLETIGNKFDGRLEADSG
                     WTNIFIKPGYTFRIVDAFSTNFHLPKSTLVMLVSAFAGREFTLDAYKHAVKERYRFFS
                     FGDAMFIQ"
     gene            2939..3412
                     /locus_tag="VCA20_001160"
     CDS             2939..3412
                     /locus_tag="VCA20_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEP1834992.1"
                     /translation="MNKIESRHQLILSLIMEKKIHTQQELQELLEVNGVSVTQSTLSR
                     DIKMLNLVKVNEDDSSHYVINPIAPTRWEKRLRLYMEDALVMLKPIQHQVVLKTLPGL
                     ANSFGSILDAMEIPQIVATVCGDDVCLIICEDVEGAQACFEHLKQFTPPFFFSKL"
     gene            complement(3702..7961)
                     /locus_tag="VCA20_001161"
     CDS             complement(3702..7961)
                     /locus_tag="VCA20_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HEP1834993.1"
                     /translation="MKKETSKFTKKVILGQLLICTSFLVWAGQTVAAEEVSQGEPVAI
                     NNETPPVIEAKPLVEATPSTETEPSTPEEKQEEPQVADKNVVEGKKPVTNSQHQMVSE
                     LSTVPSVSIKNPDAATDGNAYGSSDDANSNTKIIAGKETGESDNGYSKWDHVYVQYDF
                     EKEVPVHTVKIHRNTYDNAVSTFKDVKVELSTSADFSPETTEVIFKQADVEETIANKG
                     QPQVIQLPASINARYIRIWGRGHYIQNTNSSWKGYSNGVLFNEIEVLAQVEADAGTNE
                     EPQTELVNLAKLKKPYFYGKAPTNPEAINDGKFDENYTTQESIGNQAYLQYEFRNVYT
                     IEEIKVKLAPGEYRNFRIDLDDDYNDGNNYNTIYKNENFTIDKEQIITIKTPKNSKAR
                     YVRFRGTRVDDTPLRYSEIQIMGRGKSYDESAPEYVPPKSEYDTLVWSDEFNGDKIDE
                     SKWQIIDGMWNHAAIYNRKAVSIKKDGDKSYLSIRSTNHKDKKALVEAVGHDRYDDKE
                     LPDKVTWSSGRLESKDKYSFQFGRMAVRAKVNDSKGIWPAIWMLAQDETGHDEIDVLE
                     YLGQNPWDAWTTNHFGILAKNKKSDGSAYKNYEAWSQEFHVYEVEWTPDVIKWYIDGK
                     FVFQTDRGKDRDGMHTRPMFPILETQVGDGWVGDVDYKRNMTKQDSEYLVDWVRIYQK
                     ADQSKVRFDNLDDAKPNDYVIKPQQKLGHLVTVSNGKAAHENKNNFYYGGQPRYETSR
                     LYADGDGENALVYKIANPEALHLTTYYKTLEDYSVYNKEAGANEGKSVRKHLINAKDG
                     EIDFTVYSSVDGKSWNKENVTVVDNFVEATPAYARTTFDIRNIRKGTKFVKIVFPTVK
                     GISYKLEDGTEKGLQASDVQLAKVTFVAAHPEAEPEKPIVDQEKLALSKVFSLDAGRK
                     YFSVNQIKEIIDLISSYGYTDMNLLLGNDGLRFLLDDMNLTVGDKVYDSEAVRKAIIN
                     GNKHYYDDPNGTTLSQSEMDDILAYAKEKNISIIPTINSPGHMDAIVEAMEELGIKSP
                     KFSYNGKTSARTVDLKNDQAVAFTKALIDKYASYFAGKSKIFNIGLDEYANDATNASG
                     WHILQTQGDYGKFIQYANDLAAIVKKHGLEPMAFNDGIYYNHDTSSGTFDKDIIASFW
                     TGGWGGYDVASSKFLHEKGHKILNTNDAWYYVIGREAEGLGWYNLDQGLRGTKSTRFD
                     QVPKSENTKVPIIGSMVAVWADDPSKPYSYDSIKKLLHSFTENNSDYFLAEYRDLREE
                     VGKIPTDLNSYTPESVATLKEVLNKIDWKLSRNNQAFVDGYLAEVKAAREALVPVTAK
                     GDSVKAEPLPEGKLPLITAKGKSVQAEPRPAFKGYLTSNYGPSVAPLSLVSKPSSEQS
                     RSQQAASQKKELPNTGAVDGLGFSLLGMLGLAFASRRRKEG"
     gene            complement(8332..9672)
                     /locus_tag="VCA20_001162"
     CDS             complement(8332..9672)
                     /locus_tag="VCA20_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEP1834994.1"
                     /translation="MRTSFIQSSHQEACIQAIQELVAFPSVLQEHQADTPFGQAIQDV
                     LEHTLALTEKMGFKTYLDPAGYYGYAEIGQGEELLAILCHLDVVPAGDLSQWQTPPFE
                     AVVEGDYIIGRGVQDDKGPSMAALFAVKALLDAGVQFNKRIRFIFGTDEETLWRCMNR
                     YNQLEEVATMGFAPDSSFPLTYAEKGLLQAKLHGPGHPCLSIEAGTAYNVVPAKASYS
                     GHLLAGVIAELDQLGFDYETKDDQVTVLGISRHAKDAAEGVNAIVRLAKALEHFESHP
                     ALDFIVNAVGEDATGFKLFGDVTDEPSGTLSFNIAGLTISAKKSEIRLDIRIPVTAYK
                     EALVSTLQAKAQSCGLTYEEYDYLASLYVPLDSQLVSTLMSVYQDKTGDLTSEPISSG
                     GATFARTMPNCVAFGACFPDTEQTEHQENERMPLEDLYKTMDIYAEAVYRLAAE"
     gene            complement(9791..11293)
                     /locus_tag="VCA20_001163"
     CDS             complement(9791..11293)
                     /locus_tag="VCA20_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEP1834995.1"
                     /translation="MSEKVKKGFKLPSSYTILMLIIAFMAVMTWIIPAGQYQVDEAGR
                     LVAGTYEKVAQNPQGIYDVFMAPVRAMLGHGATEAAINVAFFIIMVGAFLGVVNETGA
                     LDVGIASIVKRFKGREKMLIYILMPLFALGGSTYGMGEETMAFFPLLVPVMMAVGFDS
                     ITGVAIILLGSQIGCLASTVNPFATVVASDAAGVSVADGMIWRLVFFVVILLMGVLFV
                     ANYAEKVKNDPTKSLVYKQREADMQHFNVTATQEVNAELSPAQKRVLWVFVLTFVLMI
                     CSFIPWEDLGVTIFTQFKDWLIGLPFIGQVIGSSTAALGTWYFPEGAMLFGIAGIVVG
                     LVYGMSEERLVKSFMFGAADIFSVALICAIARGIQVIMNDGMITATILHMGEEGLQGL
                     SSQVFIILTYLFYLPMSFLIPSSSGLAGASMGIMAPLGEFVNVPASLVITAFQAASGL
                     LNLVAPTSGIVMGALALGRVEIGTWYKFVGKLIVGIMIASIAILVIATFF"
     gene            complement(11636..12583)
                     /gene="arcC"
                     /locus_tag="VCA20_001164"
     CDS             complement(11636..12583)
                     /gene="arcC"
                     /locus_tag="VCA20_001164"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEP1834996.1"
                     /translation="MANRKIVVALGGNAILSSDPSAKAQKEALVQTAKHLVKLIKNGD
                     DLIITHGNGPQVGNLLLQNLAADSEKNPAFPLDSLVAMTEGSIGFWLQNALENELLKE
                     GIEKDVASVVTQVIVDKNDSAFENLTKPIGPFYTEEEAKAEAEKTGATFKEDAGRGWR
                     KVVASPKPVGIKEIGTIRTLLNAGEVVVAAGGGGIPVVQEADGTLTGVEAVIDKDFAS
                     QCLAELVDADLFIVLTGVDYVFVNYNKPDQEKLETVTVAELEEYIKQNQFAPGSMLPK
                     VEAAIAFVNNKPQSKAVITSLENLGALIESDSGTIIVKD"
     gene            complement(12701..13714)
                     /gene="argF"
                     /locus_tag="VCA20_001165"
     CDS             complement(12701..13714)
                     /gene="argF"
                     /locus_tag="VCA20_001165"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000195397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEP1834997.1"
                     /translation="MTNVFKGRHFLAEKDFTRAELEWLIDFSAHLKDLKKRNIPHRYL
                     EGKNIALLFEKTSTRTRAAFTVASIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRMF
                     DGIEFRGFSQKMVEELAEFSGVPVWNGLTDAWHPTQMLADYLTVKENFGKLEGLTLVY
                     CGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEEEVVALAEGFAKESGARVLITDNA
                     DEAVKGADVLYTDVWVSMGEEDKFAERVALLKPYQVNMELVKKAENENLIFLHCLPAF
                     HDTNTVYGKDVAEKFGVEEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGDPFV
                     PRV"
     gene            complement(13733..14173)
                     /locus_tag="VCA20_001166"
     CDS             complement(13733..14173)
                     /locus_tag="VCA20_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEP1834998.1"
                     /translation="MPSIERTTIGSEEWQRAASIYVRYKVFVLERGIAKEDEFDQNDT
                     EGRVYANLFIGEEPVSTGRFLPVGPGQARLTRIATLPNFRGKGYGKMIISSLEAYARE
                     AGFHQLNIHSELTAKTFYESVGYQATSEVYQEDGEWWQTLTKFI"
     gene            complement(14173..15405)
                     /gene="arcA"
                     /locus_tag="VCA20_001167"
     CDS             complement(14173..15405)
                     /gene="arcA"
                     /locus_tag="VCA20_001167"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEP1834999.1"
                     /translation="MMSNHPIHVFSEIGKLKKVMLHRPGKEIENLMPDYLERLLFDDI
                     PFLEDAQKEHDAFAQALRDEGVEVLYLEKLAAESLVTPEIREQFIDEYLEEANIRGRA
                     TKKAIRKLLLSIEDNQELVEKTMAGVQKAELPEIPSEEKGLTDLVESSYPFAIDPMPN
                     LYFTRDPFATIGNAVSLNHMYSETRNRETLYGKYIFTHHPEYGGKVPLVYNREETTRI
                     EGGDELVLSKDVLAVGISQRTDAASIEKLLVNIFEQHVGFKKVLAFEFANNRKFMHLD
                     TVFTMVDYDKFTIHPEIEGDLRVFSVTYENDTLHIEEEHGDLAELLAANLGLEKVELI
                     RCGGGDMVAAGREQWNDGSNTLTIAPGVVVVYKRNTITNAILESKGLRLIKIGGSELV
                     RGRGGPRCMSMPFEREDI"
     gene            complement(15663..16358)
                     /locus_tag="VCA20_001168"
     CDS             complement(15663..16358)
                     /locus_tag="VCA20_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEP1835000.1"
                     /translation="MISNEQYDYLRTHPTFERLTRDSFDQLAKHIRFRKIPKGQIFFY
                     AEDPRDYLFVLYKGYARIEQYDETDSFTYLDYIRQGGAFPFGDMFQDKPYHYTAIAIT
                     DLEYFIVPMVLFETLSKINPSQMAYICQKLSKVLRFQELRLRNAMRSKASDRVVQALA
                     LLYWDMCQREQFATLPFEIHIQEISRLAATTRETVSHVLKQLKQDGKIAYSHKQLTYL
                     DIDYFLENLTETH"
BASE COUNT         4798 a         3678 c         3001 g         5130 t
ORIGIN      
        1 agaagtgatg acaaatttgt gataattggc gtacactcca ttgcaaatga tgagatttag
       61 cggtatactg taagtggctc atttggacta acctcctgta tgtttcgtca cttacagtat
      121 agtccatttg ggctttttat tgtagtttga aatgaactag caccacgggt atttcctatt
      181 tcttcaagga taagctcagt cacgatgcca tgaattttcc gcacatcgtt gaacggtgcc
      241 agctgattac tattatcacc tttggtgaaa cagtcattgc gattttgaaa aattatccga
      301 ttcagacgca tttcctcact ggtgttctct tcttcttggc aatggcgttc tcctttatgt
      361 tttacatttc gcaaacctat ctcaatatca accaccatca aaagacaaat gtagctacct
      421 tactctacgc tcatatggta ttggttttgg gtatcaactt cttcacagtt tctgtggaag
      481 tcttacctgg tgaacatgtt agtttgggct tgcctttcct tctcattggt tacttcctct
      541 actttatggg aatcctcatg accagtcgct acaaccagga cctttaaaga tggtttggtt
      601 caatatgcta ttgtagtatt cactaccatc attcttctga tagccttcca ccaccatcta
      661 accttgattg ccgctattct ggttgctagt tcatttatga tgctggtaat ctctttccgc
      721 caccgcaatc gtgtacaagt tgacctcgaa aaataagaaa tccgactgcc aaaaatggta
      781 gtcggttttg tttattatgc taatttactc gctgcggctt ggtcgaggat gaggatgaca
      841 ttgtcatggt tttggaggac gcttgctggg acatcttctg tcactgcccc ttccacagtc
      901 gccttaatgg cgtcagcttt ttcaggacca taagccatga gaacaatggt cttggctgcc
      961 ataatgttgg cgatgcccat ggagatagct tgttttggta catcttctgg attgtcaaag
     1021 aagcgggcgt tggcttcaat ggttgatggc gccaattcaa ccagatgggt ctgaccatca
     1081 aatggagcac ctggttcgtt gaaaccgata tggccgttac gaccgatacc caaaatttgg
     1141 aaatcgaccg gatgttcagc taaaattttg ttgtaacgtt ctgtttcagc aactacatct
     1201 gtcgctgaac cattcggtaa ataagattgt ttaaacggtt tggcattgaa aagattttcc
     1261 ttcataaaat gaatatagga ttgatcgctc tcctcaccta aaccgacata ctcatccaag
     1321 tttacagatg tcacattcga aaagtctaaa tccgactcac gaatcaattt gtaaaattct
     1381 aacggactgg agcctgttgc caaacctaat accttggcac ctccgttcat cttttctctc
     1441 aaaatgtcta atgctacagt tgctccttca acttgattgt ttactacatg aattttcata
     1501 acacacctct ttggtatata ccatttcttg tttttcttta ttatatggta tagaccgatt
     1561 tttgtcaaga atttatactt atagagtatt acatcagcac gaataatttt aattctcttc
     1621 ccatccatca agacttaagt gtctaactat gatataatac atttatgaca gatgtgcttt
     1681 tttacttatt ttttattggt attctgttct gccttactgg ctattttatt tctaaatcaa
     1741 agatcctaaa gtttattttc tacctgattg gtagtctatt ggtggctttg ccatttgccc
     1801 tattgattta ttttacctat attttatttt agtctaagga gtgaatatga atactgcaga
     1861 tttcgatttt gatttacccg aagagcttat cgctcaagta ccccttgaaa agcgcgacag
     1921 ctctaaacta ttgatcctcg accgcgaaaa acggagcatg gttgacagtc actttgacca
     1981 tatcatcgac cagctaaatc ccggtgatgc tctggtcatg aacaatacac gtgttctgcc
     2041 tgcccgtctc tatggctaca agcctgaaac tatgggacat gttgagcttt tgcttttgaa
     2101 aaatacccaa ggtgatcagt gggaagttct tgccaaacca gcaaaacgtc tgaaggtcgg
     2161 taccacagtc gcttttggtg acggacgatt aactgctact atcgtagagg aattagaaca
     2221 cggtggaaga attgttgaat tctcctatga aggtatcttc cttgaagtct tggaaagtct
     2281 aggcgaaatg ccacttccac cttacattca taaaaaatta gaagaccgtg aacgctatca
     2341 aacagtttat gccaaggaaa acggttctgc cgcagctcca actgctggac tacatttcac
     2401 ccaagaatta ctcgaaaaaa tcgaagctaa aggagtgaag ctagtctatt tgactcttca
     2461 cgttggactt ggaaccttcc gaccagtctc tgttgataac gttgatgagc atgaaatgca
     2521 ctcggaattc tataacctat cagcagaagc tgctcaaacc ctcaatcagg tcaaagatag
     2581 cggtggtcgt atcgtcgcag tcggcacgac atccatccgc accctcgaaa ccatcggtaa
     2641 taaatttgat ggccgtttgg aagctgattc tggttggaca aatatcttca tcaaacctgg
     2701 ctacaccttc cgaatcgttg atgccttctc aaccaatttc cacctgccaa aatctacttt
     2761 ggttatgcta gtttctgcct ttgcaggtag agaatttacc ttagatgcct ataaacatgc
     2821 tgttaaagaa cgctaccgct tctttagttt tggggatgca atgttcattc aataggctgt
     2881 tatcaccaat ttattctgag ttcagatttt attttcctta catctcaagg agggccctat
     2941 gaacaagatt gaaagccgcc atcagctgat cctatctcta atcatggaga agaaaataca
     3001 tacccagcag gaattgcaag aattactaga ggtcaacgga gtctccgtca cccaatctac
     3061 cctctcccgt gacatcaaga tgctcaatct tgtcaaagtc aacgaagatg attcttctca
     3121 ctatgtcatc aaccccattg ctcctactcg ctgggaaaaa cgactgcgac tctatatgga
     3181 agatgctttg gtcatgctaa aacctatcca acatcaagtg gtgctcaaga ccctacctgg
     3241 attggccaac tcattcggtt ctattttgga tgccatggaa attcctcaaa ttgtagcgac
     3301 cgtctgcggt gacgatgtct gtctcatcat ttgtgaagat gtcgaggggg cacaagcttg
     3361 cttcgagcac ttgaaacagt ttactccacc atttttcttt agtaaattat aaaaatttgt
     3421 gttcacatac aaatacttaa ttttaaagat aatttttcaa ataaatatca ataacttccc
     3481 tttatactca taaacgaaaa tctcccgtaa taagatttat tacgggggat ttttagtagt
     3541 tgataaaaat tatcctgtta ttcatagcct tctagtttac tttttggtac aaggcaatga
     3601 gctgcttgct atacttgggt acagcaaagc gagtgaaaga aaaactagat aactatataa
     3661 ttacaattag aaagccctga acattcactc aaatggctat attacccctc cttccgccgg
     3721 cgactagcaa atgccagacc aagcatacca aggagagaga atccaagacc atctactgcg
     3781 cctgtatttg gcaattcttt cttctgagaa gctgcttgct gggagcgtga ctgctcactg
     3841 cttggttttg atacgagaga taaaggtgcg acagatggtc catagtttga tgtcaggtat
     3901 cccttaaagg cagggcgtgg ttctgcttgg acactttttc ctttagcagt aatcagaggt
     3961 agcttgcctt ctggtaaggg ctccgctttg acactgtctc cttttgctgt cacaggaact
     4021 aacgcttcac gcgctgcttt tacttctgcc aagtaaccat caacaaacgc ttggttattt
     4081 ctacttagtt tccaatcaat cttgttcaag acctctttta aagtcgccac gctctctggt
     4141 gtataggaat ttaaatcagt aggaatcttc ccaacttctt ctcgcaaatc tcggtactct
     4201 gccaaaaaat aatcagaatt gttttccgta aaactgtgca aaagtttttt aattgagtca
     4261 tagctgtaag gcttgcttgg gtcatctgcc cataccgcaa ccatactacc gataatgggt
     4321 accttagtat tctcagattt cggtacttga tcaaaccttg tagatttagt tcctcgaaga
     4381 ccttgatcaa ggttatacca tcccaaacct tctgcttcac gtccaataac atagtaccaa
     4441 gcatcatttg tgttaagaat tttatgccct ttttcatgaa ggaattttga ggaagcgaca
     4501 tcatatcctc cccaacctcc tgtccagaaa gaagcaataa tgtctttatc aaaagtaccc
     4561 gaactagtat cgtggttata atagatacca tcattgaaag ccattggctc taaaccatgc
     4621 ttcttgacga tagctgctaa atcattggca tattgaataa acttaccata atcaccttgt
     4681 gtctgaagaa tgtgccatcc agaagcattt gtagcatcgt tagcatactc atctaagcca
     4741 atattgaaaa ttttagattt gcctgcaaag taactagcat atttatcaat taaggcttta
     4801 gtaaaagcaa ctgcttggtc attttttaaa tcaactgtac gtgcagatgt tttgccatta
     4861 taactaaatt ttggcgattt tatccccaat tcttccattg cttctacaat cgcatccata
     4921 tgacctgggc tattgatagt tggaataatg gaaatatttt tttccttagc ataagcaaga
     4981 atatcatcca tctcactctg acttaaggtt gtaccgtttg gatcgtcata ataatgctta
     5041 ttaccattta ttattgcttt tctaactgct tcactgtcat acaccttatc tccaacggtc
     5101 aaattcatat catctaataa aaatctcaga ccatcatttc ccaataagag gttcatatcc
     5161 gtgtaaccat aagaagaaat taaatcaata atctccttta tctgatttac agagaaatac
     5221 ttgcgaccgg catcgagtga aaaaactttg gacaaagcta gtttttcttg atcaacaata
     5281 ggtttttctg gttctgcttc aggatgagca gctacaaaag ttacttttgc taactggaca
     5341 tcactggctt gcaggccttt ctctgttcca tcttccaact tataggaaat acctttgacc
     5401 gttggaaaga cgattttcac aaatttagta ccttttcgaa tgttgcgaat atcaaatgta
     5461 gtgcgtgcat aagctggggt tgcttctaca aaattatcca ctacagtaac attctcttta
     5521 ttccagcttt ttccgtcaac agatgaatac acagtgaagt caatttcacc gtcttttgca
     5581 tttatcaaat gtttgcgaac agattttcct tcattagccc ctgcttcttt attatagaca
     5641 gaatagtctt caagtgtttt gtaataagtc gttaagtgca aagcctctgg attagcaatc
     5701 ttgtaaacta gtgcattttc gccatcacca tcagcataga gcctgcttgt ttcgtaacgt
     5761 ggttgacctc catagtagaa attatttttg ttttcatgag ctgcttttcc gttagagact
     5821 gttacgaggt gaccaagttt ttgctgcggc ttaataacat aatcattagg cttggcatca
     5881 tctagattat caaaacgtac cttactttga tctgcttttt gataaattcg aacccaatca
     5941 accaagtact cactatcctg tttagtcatg ttgcgcttgt aatcaacatc accaacccag
     6001 ccatcaccaa cttgggtttc cagtatcggg aacatagggc gggtgtgcat accatcccta
     6061 tccttaccac ggtcggtttg aaaaacaaat tttccatcaa tataccattt gatgacatct
     6121 ggagtccatt cgacttcgta gacatggaat tcctgactcc aagcttcgta attcttataa
     6181 gctgagccat cagatttttt attttttgcc aaaataccaa aatggttagt tgtccatgca
     6241 tcccatggat tttgaccaag gtattccaaa acgtcaattt cgtcatgccc ggtctcatcc
     6301 tgtgccaaca tccaaatagc aggccagatt cccttagagt catttacctt agctcgaaca
     6361 gccattcgac caaattggaa agaatactta tctttagatt ctaggcgacc agaagaccat
     6421 gttactttat ctggtaattc tttatcatca taccgatcgt gcccaactgc ttcaaccaaa
     6481 gcttttttat ccttatggtt tgtgctacgg atagacaaat agctcttgtc accatctttt
     6541 ttgatagaaa ctgctttacg attgtatata gccgcatgat tccacatgcc atcaataatt
     6601 tgccatttac tttcatcaat cttgtcgcca ttaaattcat cagaccagac taatgtatcg
     6661 tattcagatt ttggtggaac gtattctgga gcagattcgt cataagattt tcctcgtccc
     6721 ataatctgaa tttctgaata gcgaagagga gtatcatcta cgcgcgtacc tctaaagcga
     6781 acataacgcg ccttagaatt ttttggagtt ttaatcgtta tgatctgttc cttgtcaatg
     6841 gtgaaatttt cattcttata gattgtatta taattattac catcattata atcatcatcc
     6901 aaatctatcc ggaaattacg atactctcca ggagctaact taaccttgat ttcctcaata
     6961 gtatagacat ttctaaattc gtattgaaga taagcttggt tccctattga ttcttgagta
     7021 gtataattct cgtcgaactt tccatcgtta atggcttctg gattagttgg agccttacca
     7081 tagaaatatg gttttttcaa tttagcgagg ttgactagct cggtttgtgg ttcttcattc
     7141 gtaccagcat ctgcttcaac ctgcgccaag acttctatct cgttaaagag aacgccatta
     7201 ctatatccct tccatgaact gtttgtattt tggatataat gcccccgtcc ccaaatacga
     7261 atataacgag cattgattga tgctggtaat tggatgactt gaggctgtcc tttatttgca
     7321 attgtttctt ctacatctgc ctgtttgaaa atgacttctg tggtttcagg agaaaaatct
     7381 gcacttgtag acaattccac tttaacatct ttaaaagtag acactgcatt gtcataagta
     7441 ttacgatgaa tttttacagt atggacggga acttcctttt caaaatcata ctgaacgtaa
     7501 acatgatccc acttggagta accgttatct gattcgccag tttctttacc agcaatgatt
     7561 ttggtattgc tattggcatc gtcgctacta ccgtaagcat tcccatcggt agctgcatct
     7621 ggatttttta tagaaacaga agggaccgtt gataattccg aaaccatctg atgctggctg
     7681 ttagttacgg gctttttccc ttctaccaca tttttatcag ctacttgtgg ttcctcctgt
     7741 ttttcttctg gagttgaagg ttctgtttca gttgatggag tagcttccac aagcggtttt
     7801 gcttcaataa caggtggagt ttcattgttg attgctactg gttctccctg agatacctcc
     7861 tctgctgcaa cagtctgccc tgcccatacc aaaaaactgg tacagataag aagctgacca
     7921 aggatgactt ttttcgtaaa cttacttgtt tcttttttca ttatttcctc ctataaagtc
     7981 tgcaagttta tcctacctat agccaaaata aagcagtttg taaacctatt tagtctcgta
     8041 taagactcaa aattcagttt actggttgat gtgcgatcgc atttctgtca caaatataac
     8101 atgtaagcgt tttattgtag aatagtctaa cttgacttat tctatatgca gatggggtct
     8161 atttctaaaa cagcatataa taagtcacac tttcatatat ttttgtatac ttattgtaaa
     8221 cagtgacaca tttctatctc atctttttaa tgatattctt cataaaaaaa gcccttagat
     8281 atttcactaa gagcttttaa actgtaattt aatagtcaat gctgataatg gctattccgc
     8341 agctaggcga tagactgcct cagcataaat atccattgtt ttatataggt cttctaatgg
     8401 catgcgttca ttttcctgat gctctgtttg ttctgtatct gggaaacatg caccaaaggc
     8461 cacacagttt ggcatagtac gagcaaaggt agccccacct gacgagattg gctcgcttgt
     8521 taaatctcct gtcttatctt gataaacaga catgagcgta gataccagct gactgtctaa
     8581 tggcacatac aaggaagcaa ggtagtcgta ctcttcatag gtcaagccac atgattgcgc
     8641 tttggcttgt aaagtagaaa ctagggcttc tttatacgca gtgacaggaa tacgaatgtc
     8701 caagcgaatt tctgattttt ttgcgctaat ggttagacct gcaatattga aactgagtgt
     8761 ccctgatggt tcatcagtca catcaccaaa aagcttaaat cctgtggcat cttcaccaac
     8821 tgcattgacg ataaagtcca aggctggatg actttcgaaa tgttccaaag cctttgccaa
     8881 acgaacgatg gcattgaccc cctcagcagc atccttggcg tgccgcgaaa taccaagaac
     8941 cgttacttga tcatctttgg tttcataatc aaagcctaat tgatctaatt ccgcaataac
     9001 tcctgctaat aaatgaccag aatagcttgc cttggcaggt actacgttat aggcagttcc
     9061 agcttcgata ctcagacaag ggtgacctgg accatgcaat ttggcctgta gtaatccttt
     9121 ttcagcataa gtcaaaggga aggaagaatc tggcgcaaag cccatagttg ccacttcttc
     9181 caactgattg tagcggttca tacaccgcca taaggtctct tcatctgttc caaaaatgaa
     9241 acgaatccgt ttgttaaatt ggactcccgc atcaagaagt gcttttactg caaataaggc
     9301 cgccatcgaa ggacctttat catcctgaac cccgcgtcca ataatataat ctccctctac
     9361 cacagcctca aaaggcggcg tctgccattg actaagatca ccagcaggta ccacatctaa
     9421 gtggcaaagg attgctaata attcttcgcc ttgcccaatt tcagcatagc cataatagcc
     9481 agcagggtct aaataagttt taaaacccat tttttccgtc aaagccaacg tgtgctccag
     9541 aacatcctga atcgcttgac caaatggcgt atctgcctga tgttcttgca aaacagaagg
     9601 aaaagcaacc agttcttgga tggcttgaat acatgcctcc tgatgcgatg actgaataaa
     9661 acttgttctc attgtgtccc ctttcaacat aataagatat gatatatttt ctagcactat
     9721 tctatcataa aaggaaaaac tgagccaaat tttatctgta ttcatgataa aatctgaccc
     9781 agaatttcga ttagaagaat gtcgcaatga ccaaaatagc tatggaggca atcatgatgc
     9841 ctacgattaa cttacctaca aatttatacc aagtaccaat ttcaacacga ccaagtgcca
     9901 aggctcccat tacaatacct gatgttggag ccaccaagtt caagaggcct gatgcagctt
     9961 ggaaagctgt gataaccagg cttgctggta catttacaaa ctcaccaaga ggtgccatga
    10021 tacccataga agcccctgca agacctgatg aagatggaat caagaaggac attggtaggt
    10081 agaagagata ggtcaaaatg atgaatactt gtgaagacaa accttgcaat ccttcttcac
    10141 ccatgtggag aatagtggca gtaatcatac catcgttcat gatgacttga ataccacgcg
    10201 cgatagcaca aatcaaagct acgctgaaaa tatcagccgc accgaacatg aaggatttta
    10261 ccaaacgttc ttcactcata ccataaacta aaccaactac aatccctgcg ataccaaaga
    10321 gcattgctcc ttctgggaag taccaagtac caagagcagc tgttgaagaa ccaatcactt
    10381 gaccgatgaa cggaagacca atcaaccaat ctttgaattg tgtaaagata gtaacaccca
    10441 agtcttccca tggtataaag ctacaaatca tgagaacaaa tgttaataca aatacccaga
    10501 gaacacgttt ttgagctggt gataattctg cattcacttc ttgagttgct gttacattaa
    10561 agtgttgcat gtccgcttca cgttgtttat aaaccaagga cttagtcgga tcgtttttca
    10621 ctttctcagc atagttggct acaaagagaa cccccatcaa gagaataaca acgaagaaga
    10681 ccaagcgcca aatcataccg tccgcaacac ttacacctgc tgcatcagag gctacaaccg
    10741 ttgcaaatgg gtttacagta gaagccaaac agccgatttg agaacctagc aagataatgg
    10801 ctacaccggt aatactatca aaaccaaccg ccatcataac aggaacaagg agtgggaaga
    10861 aagccattgt ttcttcaccc ataccatagg ttgagcctcc caaggcaaac aaaggcatga
    10921 ggatgtaaat caacattttt tcacgacctt tgaagcgttt tacaatagaa gcaatcccta
    10981 catcaagtgc acctgtttcg ttaactacac caagaaaggc cccaaccatg atgatgaaga
    11041 atgctacgtt aatcgctgct tcagtcgcac cgtgaccaag catggcacga actggagcca
    11101 taaaaacatc gtaaatccct tgtggatttt gagcaacctt ctcataggta ccagccacca
    11161 aacgtccggc ttcatcaacc tgatactgac cagctggaat aatccatgtc atcactgcca
    11221 taaaggcaat aatgagcatc aatattgtat aagacgaagg caatttaaag ccttttttca
    11281 ctttttcgct catcgcaaaa acctcctata atattttgaa agtttatccc ctatttaagg
    11341 gaaattgtat ccgtaataac cgtatcaaat atccacctat cttcaagaga tgtctatctc
    11401 cttcagtcta ttatctgaaa tcattgctta tagatagata cttgatagga aatggatgag
    11461 aggggaggga gcttagacgc ccagaccttc atgtttagat taaagggaac ccttgtggga
    11521 ctatcaacac ttccactccc ctaactccat caataaataa cccgtctagt ttaaaaacta
    11581 tatttgcatg cagagtacat caataaatag aagcctcaac gatacataca gctgcttagt
    11641 ctttgacaat gatagtgccg ctatcggatt caatcaaagc gcctaagttt tctaatgagg
    11701 taatgactgc ttttgattgc ggcttattgt ttacaaaagc aatagctgct tcaactttcg
    11761 gtagcatgga acctggtgcg aattgatttt gtttgatata ttcttccaat tcagccacgg
    11821 taactgtttc taatttttct tggtctggtt tgttgtagtt aacaaagaca tagtctacac
    11881 ctgtcaagac aataaacaag tctgcatcaa ccaattctgc caaacattgg gaagcaaagt
    11941 ccttatcgat aactgcttcc acaccggtca gggtgccatc tgcttcttgg acaactggaa
    12001 tacctccgcc tccagcagct acgactacct cacctgcatt gagaagggta cgaatggtac
    12061 caatttcttt gattcctaca ggttttggcg aagcaacgac tttgcgccag ccacgaccgg
    12121 catcttcttt gaaggttgca ccagtctttt ctgcttctgc tttcgcttct tcttctgtat
    12181 agaatggtcc aatcggcttg gtcaaatttt cgaaggcaga atcattctta tccacgataa
    12241 cctgggtcac aacagatgct acatcttttt caatgccttc cttgagcaat tcattttcca
    12301 aggcattttg caaccagaaa ccaatcgaac cttctgtcat ggctaccaaa ctgtctagtg
    12361 gaaaagctgg gttcttttcc gaatcagcag caaggttttg aagaagtaag tttccaacct
    12421 gaggaccatt accatgcgtg ataataaggt catccccatt ttttatcaat tttacaaggt
    12481 gcttggctgt ttgtaccaag gcctcttttt gtgctttagc cgatgggtca gacgataaaa
    12541 tggcattgcc acccaaggct actacaattt tacgatttgc catatctctt gctttttcct
    12601 cactttcgcg aaatagtaat cgcttacaca tctaccctca aaataagggg gctgggtgcc
    12661 agcccccgga ggttgcgatg cgttattaga tagttaatta ttacacacgt ggaacaaatg
    12721 gatcccctaa agtagccgcc atcactgctt taatagtgtg catacggttt tcagcttggt
    12781 cgaagtgacg agcatatttg ctacggaata cttcgtctgt tacttccatt tcttctacac
    12841 cgaatttttc agcgacatct ttaccataaa cagtgtttgt atcatggaag gcaggcaagc
    12901 agtgcaagaa gatgagattt tcattttctg cttttttcac caattccata ttcacttggt
    12961 atggtttcaa gagggcaacg cgttctgcga atttgtcttc ctcacccatt gatacccaaa
    13021 cgtctgtata gagaacatct gcgcctttga ctgcttcgtc agcattgtca gtaatgagaa
    13081 cacgtgctcc actttctttc gcaaaacctt cagccaaggc aactacttct tcttctggga
    13141 agagttcttt tggagagaag atatggacat tgacaccaag gatagcacct gtcaccaata
    13201 gagagttggc aacgttgtta cggccatcac cacagtaaac cagagtcaag ccttccaact
    13261 taccaaagtt ttctttgaca gtcaagtagt cagccaacat ttgagttggg tgccacgcat
    13321 ctgtcaatcc attccatact ggcacaccag agaattctgc caattcttcc accatttttt
    13381 gactgaaacc acggaattca ataccgtcga acatacgtcc caaaacttta gcagtatctt
    13441 ctgtagattc tttcttacca agttggatat catttgcacc aagatattct ggatgggcac
    13501 caaggtcaat agatgctact gtgaaggcag cacgagtacg tgttgatgtt ttttcaaaca
    13561 agagagcaat atttttacct tccaaataac ggtgtggaat attgcgtttt ttcaaatctt
    13621 tcaaatgagc tgagaaatca atcaaccatt ccaattctgc gcgtgtgaaa tctttctctg
    13681 caaggaaatg tctaccttta aatacgtttg tcattgtctc ttctcctaat gtttaaataa
    13741 attttgtcaa tgtctgccac cattctccat cttcttgata aacttcagaa gttgcttggt
    13801 agccaactga ctcataaaaa gtctttgcag ttaactccga atgtatattc aactgatgaa
    13861 agcctgcttc acgtgcataa gcttccaaag agctgataat catttttcca tagccttttc
    13921 cacgaaaatt cggtagagtg gctattcggg ttagacgagc ttgaccagga ccgacaggaa
    13981 gaaaacgtcc cgtcgaaaca ggctcttcac caataaagag attggcgtaa actcttcctt
    14041 ccgtatcatt ctggtcaaat tcatcttcct ttgcaatacc acgctcaagg acaaatacct
    14101 tatagcggac atagatagag gctgcgcgtt gccattcttc cgaaccgatt gttgttcgtt
    14161 ctatacttgg cattagatgt cttcccgttc aaacggcatt gacatacaac gaggtccacc
    14221 acgaccgcga accaattcgc ttccgccaat cttaatcaaa cgaagccctt tggactcgag
    14281 gattgcattg gtaatggtat tgcgtttgta aacaactacc acacctggtg caatagtcaa
    14341 tgtatttgaa ccatcattcc attgctcacg accagcagca accatatcac cgccaccaca
    14401 acggatcaat tctacttttt caagaccaag gttcgctgcc aaaagttctg ccaagtcacc
    14461 atgttcttct tcaatatgaa gtgtatcatt ttcataggtt acagagaaga cacgaaggtc
    14521 accttcaatt tctgggtgga tagtaaactt atcatagtca accatggtaa atactgtatc
    14581 caagtgcatg aatttacggt tgttagcaaa ttcgaatgcc aatactttct tgaagccaac
    14641 atgctgttca aagatattta ccaatagttt ttcaattgac gcagcatcgg tacgttgtga
    14701 aatacctact gccaaaacat cttttgaaag aaccaattcg tccccaccct cgatacgagt
    14761 agtttcttca cgattgtaaa ccaatggaac ttttccacca tattctgggt gatgagtgaa
    14821 gatatattta ccatacaagg tttcacggtt ccgtgtttct gaatacatgt ggttaagaga
    14881 aacagcatta ccaatcgtag caaatggatc ccgtgtgaag taaaggttcg gcattgggtc
    14941 tattgcaaat gggtaagagg attcaaccaa atctgtcaaa cctttttctt cagaagggat
    15001 ttctggcaat tctgcctttt gaacaccagc catcgttttc tcaaccagtt cttgattatc
    15061 ttcaatagac aacaacaact tgcgaattgc cttcttagtc gcccgaccac gaatgttagc
    15121 ttcttcgaga tattcatcaa taaattgttc acgaatttca ggtgtaacaa gtgactcagc
    15181 agctaatttt tccaagtaga gcacttctac accttcatca cgaagagctt gagcaaaggc
    15241 atcatgctct ttctgtgcat cttctaagaa tggaatatca tcgaaaagta ggcgttctag
    15301 atagtcaggc ataaggtttt caatttcctt acctggtctg tgtaacataa cttttttcag
    15361 cttaccaatt tctgaaaaaa catgaattgg atgatttgac atgatctgtc ctccttgtag
    15421 atttatacat gatgggaaac gaggaggacc gtacgctttc ctgcctctca tttcctcttt
    15481 acatccatag tttaacagag caaaaaaaca aaaacttgaa aacgctcaca tttattttga
    15541 gaaattcttc acatttttaa ccttatttat aaataattat cgattatatc gcctatattc
    15601 tgcataattc tttccgttat atcattatat gaatttttat tttttataaa aaaacagaaa
    15661 cttcaatgag tttctgtcaa attttctaag aaataatcaa tatctaaata agttaactgt
    15721 ttatggctat aagcaatttt accatcctgc ttcagttgct tcaaaacatg gctaaccgtt
    15781 tcccgtgtcg tagctgctaa acgggaaatc tcttgaatat gaatttcaaa tggcaaggta
    15841 gcaaactgct ctcgctggca catatcccaa taaagaagag ccaaggcttg aacgactcga
    15901 tctgatgcct tggaacgcat cgcatttcgc aagcgcaact cttgaaaacg aagcactttg
    15961 gataattttt gacagatata agccatttga ctcggattta tcttcgacaa ggtttcgaac
    16021 aagaccatcg gtacaataaa atactctagg tccgttatgg caatggcagt ataatgataa
    16081 ggtttgtcct gaaacatatc tccaaaagga aaggcaccac cctgccgaat atagtctaaa
    16141 tacgtaaaac tatccgtctc atcatattgc tcaatccgag catagccctt atacaataca
    16201 aatagataat ctctaggatc ctctgcgtaa aaaaagattt gtcccttagg aatctttcga
    16261 aaacgaatat gcttagctaa ttgatcaaag ctatcacgtg tcaaacgttc aaaagttgga
    16321 tgtgtcctta gataatcata ttgttcgttg cttatcatca aaactccttt ctagctatcc
    16381 ttctatctat tttaacatat attaacgttc tatgctaatc attactgaaa aattcataaa
    16441 acaggatcat tcctgtgaag aggaatacta tcacagctaa tacaaaaaac tggtcctctc
    16501 gaaccagtct tagtatcgtc gaatgaattg actttcagac aaggaactga ggtgcaagca
    16561 atactagagt acggcaagcc gaaagggact atgtatcaaa gctaatt
//
LOCUS       DATUCG010000047        16607 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.048, whole genome shotgun sequence.
ACCESSION   DATUCG010000047 DATUCG010000000
VERSION     DATUCG010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16607
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.048"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            326..508
                     /locus_tag="VCA20_001169"
     CDS             326..508
                     /locus_tag="VCA20_001169"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835001.1"
                     /translation="MKNKNFYAVLIPVVLWIVFKICANLSDENIKDYFNILAVSNPGL
                     IIAFAIFTNKEQNKEK"
     gene            731..1420
                     /locus_tag="VCA20_001170"
     CDS             731..1420
                     /locus_tag="VCA20_001170"
                     /inference="COORDINATES: protein motif:HMM:NF014566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEP1835002.1"
                     /translation="MINILKKCGYVLISIFGFMFLSIFTQTPVVSTFSDDRIHVSNNL
                     TGDTIRIIVCIAFVLLSLWFLSSFAKRLDITLKLLQFKQGKINKKSLVIYSSVTLFLT
                     LVYVTILYVGNSYNVGSVPTQILEMKERPIVFILYLLTTIVTQLILEEILYRGILQEK
                     LSRYIGWGSIVVTSLIFSYSHDYQSAFNSKLISSLIYGLAYYSTNDIRIPSIAHCLHN
                     FAVVMVVILLG"
     gene            1461..1685
                     /locus_tag="VCA20_001171"
     CDS             1461..1685
                     /locus_tag="VCA20_001171"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835003.1"
                     /translation="MENKHQIMPIKETNKIMQFLQGLVVGILVTAVLIHLVAWLVLGK
                     TVDVFSSDFYPTVFIALYFLMKVNETKKED"
     gene            1689..2390
                     /locus_tag="VCA20_001172"
     CDS             1689..2390
                     /locus_tag="VCA20_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEP1835004.1"
                     /translation="MKRVKNILKFIGLILVIVAINITPMRLIAIQDSMSGAMQWVSGI
                     GYLVIATAIVMWTWKRYKKGLPDQQKRFTFTWKDFGFALLFFLAGRVVGIGGTLLTQL
                     VTGNATTANDAALLATNEQLAKMFPLYFVAFHIAIGVFAPFMEELVFRGLFSSYFFKE
                     NQKWLKLIISSAVFALLHAIHPIELLLYFLLGAVCYLAYARRGNIVDAILVHILNNSL
                     MVIFSIIGYLLVLFG"
     gene            2412..2606
                     /locus_tag="VCA20_001173"
     CDS             2412..2606
                     /locus_tag="VCA20_001173"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835005.1"
                     /translation="MTMMEKLDKKYQEFETAYESSPFAMKFLVWVGVALTLMTLGSVL
                     VPAIYSAGQDFGEFLYSVFG"
     gene            complement(2701..3042)
                     /locus_tag="VCA20_001174"
     CDS             complement(2701..3042)
                     /locus_tag="VCA20_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEP1835006.1"
                     /translation="MVYEVHLSAKAEQDLEEIYQYYVREFSESSARKVLASLSTAILT
                     LEIFPEGYIDLDARLGRALFPEGKTRMIPGKQHLIFYLIRDSRVDVLRIRGARTDYLN
                     NLDNLFRQTLK"
     gene            complement(3032..3319)
                     /locus_tag="VCA20_001175"
     CDS             complement(3032..3319)
                     /locus_tag="VCA20_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEP1835007.1"
                     /translation="MAGLVRDRQYNFRVNAQLLEQAKIILSEKDMTLSDALNLFVERV
                     VEEQDLPIKTVEEARAEAFLDELIAELDEGYQEVLSGHTTPAREVFAKYGL"
     gene            4434..5108
                     /locus_tag="VCA20_001176"
     CDS             4434..5108
                     /locus_tag="VCA20_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEP1835008.1"
                     /translation="MKIIKSIGLAVGTLLLTLLIQFPAYVGITAFQENVLLKLAVMIG
                     LGLPILALLSVIRRGMMKHQVFSRETLNVLKIVGMTALVFTGQTCLNLFWQYLGLSLS
                     DSNAMDTVNQLNSDNFGVMALSVVLFAPVLEEFLYRGILLEKTAQYFPKHPQMVIVFS
                     ALLFAYCHTWNFSVAFLGHFITGAYLGYLYMCNRRMTDTILAHSFYNASILFLQILFG
                     LVQIGG"
     gene            5206..5481
                     /locus_tag="VCA20_001177"
     CDS             5206..5481
                     /locus_tag="VCA20_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835009.1"
                     /translation="MRKFFQDLNQEYLETRSSVGEDWLEWIIQRKMTWGMRWGLVGIL
                     MFVLQPLTYNPHFLLGCFYIGFLLGLVYVCGQLFFAARFIFRNVRKK"
     gene            complement(5495..6154)
                     /locus_tag="VCA20_001178"
     CDS             complement(5495..6154)
                     /locus_tag="VCA20_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEP1835010.1"
                     /translation="MTPNKEDYLKCIYELGQLDQKITNKLIAEKMAFSAPAVSEMLKK
                     MVAEELISKDTKAGYLLSQTALEMVASLYRKHRLIEVFLVEQLGYSPEEVHEEAEILE
                     HTVSDHFINRLDLLLEQPQTCPHGGSIPQAGQPLIERYQTRLSQLTETGNYQLVRIHD
                     FYQLLQYLEQHELAVGDLLTVTDFDHFAQTITIQYKDKELAVPTAIAQQLFIEKSNRP
                     A"
     gene            6280..7203
                     /locus_tag="VCA20_001179"
     CDS             6280..7203
                     /locus_tag="VCA20_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEP1835011.1"
                     /translation="MKKILFSFALLLSLIGLGACRPSQTTEGSSKPRVAVTTSFLNDM
                     VYQLAGDEVERDLLIPAGEDPHLYVAKSSDLSKLQKADLVLYHGLHFEGKMVEALEKT
                     GVAVSKNFNAKDLNTMDEDGEEIVDPHFWFSIPLYKSAVAVASEELQKLLPAKAEMIQ
                     KNAEKYQAQLDDLHAWVEKELSVIPKESRYLVTPHDAFNYFAASYDFTLYAPQGVSTD
                     SEVANSDMIETVNLIIDHNIKAIFTESTTNPERMKKLQEAVKAKGGQVEVVTGEGKEL
                     FSDSLAPEGEEGDTFIDMYKHNVKLMVKYLK"
     gene            7757..8527
                     /locus_tag="VCA20_001180"
     CDS             7757..8527
                     /locus_tag="VCA20_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEP1835012.1"
                     /translation="MSAIIELKDVNLAYTASQTMALEDVNLVIPRGSRTAIVGPNGAG
                     KSSLFKVILGLEKPDTGQVRLLGQEAGLERLIAQKVAYIPQSSQVNWQFPATVFEIVL
                     MGRFAHSKGMFRRPTKADRQIVEQALERLKIADLRHRQIDQLSGGQRQRVFLARALAQ
                     EAELYLMDEPLAGIDQATEIMIMDMLKEFQCEGKTSIVIHHDLTTLDAYFDHLVWLHK
                     HVIDSGPMDETLTSENYQATYGIGNGLFLGNAKGGSHV"
     gene            8520..9380
                     /locus_tag="VCA20_001181"
     CDS             8520..9380
                     /locus_tag="VCA20_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEP1835013.1"
                     /translation="MFEVFKEYSFWTVALGTVSLAVAASTIGSISVLTKQSLLGDALG
                     HASYPGVIVSFMIFQSRHPLYLLLGAVLSGYLSYVVVHWLRRKGGHSLVNALSLVSAS
                     FFGLGMVLKNAIQGNEAFAGSSQAGLQTYLFGQAAFIQLDDVILIGIISLLALVLFAF
                     FYQDYKLYLFDQTFARVIGVRVRYLQQLTMFLMICLIAVGLKLVGAILMSSFLIAPAV
                     FGLMLGKSYHKSLLLAGIVAIGSAFVGTWISSSVSGLSTGPTIIVCLTGLTLSAFVYV
                     TYVRKENGRV"
     gene            9373..10212
                     /locus_tag="VCA20_001182"
     CDS             9373..10212
                     /locus_tag="VCA20_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEP1835014.1"
                     /translation="MFEVLLILMVIASSCGLLGSILVVKNQSMLADALSHSVLLGIVL
                     GFFISHSLDSPLLIVGASLFGLLSVLAIDRLHSRKIAHDAATGLVFSFFFAVAVLLIS
                     LFARNVHLDVDLVLQGEVLFAPLHRMDVLAWSLPVSLVKSSLAWLVIVLFFAWGYHRL
                     QVYLFDSNHARLSGLRTRILEMVILILVSLTTVLAFEAIGSMTVIVFLVAPSMAALRW
                     VKSFWQLLLLGQGIAILTVALGFLVANQLDLTMSGTCAVVSLLVVCSSIILKNTWSRS
                     SDK"
     gene            10347..11448
                     /locus_tag="VCA20_001183"
                     /pseudo
     CDS             10347..11448
                     /locus_tag="VCA20_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936094.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            11872..13764
                     /locus_tag="VCA20_001184"
     CDS             11872..13764
                     /locus_tag="VCA20_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase"
                     /protein_id="HEP1835015.1"
                     /translation="MTRLQDDFYEYVNGEWAKTAVIPDDKPRTGGFTDLADEIEKLMI
                     ATTNGWLAGGNVPEDSVLQNFVAFHKQVADYETRNRLGAEPVQALIAEYKALNSFEEF
                     TSKLAEFELAGKPNFMPFGVSPDFMDAQTNVLWAYGIATILPDTTYYADGHEKGAELL
                     KTWRECQEALLPKFGFSDEEIAELLDKVLALDAIVAKYVLSNEEGSEYAKLYHPYQWE
                     DFAALAPNLPLTAFFEELLGQVPDKIIVPEERFWQAANEIYSPENWDLLKAGLVFAAT
                     RSYTSFLSDEIRILDGAYSRALSGTPQAQNREKAAYNLAQGFFSQALGLWYAGEKFSP
                     EAKADVETKVAKMIEVYKSRLETADWLAQETRDKAITKLNVIKPYIGYPEELPARYQD
                     KIVDPSKSLVENAVELIKVDIRYAWSKWNQPVNIKEWGMPAHMVNAYYNPQKNLIVFP
                     AAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDSNGASFDEHGSLNNWWTEEDYAA
                     FEARTQQVIDQFEGQDSYGAKINGKLTVSENIADLGGIAAALEAAKSEDDFSAEEFFT
                     NFARIWRMKARPEFMQMLASVDVHAPGHLRTNIQLPNFDEFHETFGVQEGDGMWRAKE
                     DRVIIW"
     gene            13867..16368
                     /locus_tag="VCA20_001185"
     CDS             13867..16368
                     /locus_tag="VCA20_001185"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEP1835016.1"
                     /translation="MSFYNHKEIEPKWQEFWAKNHTFKTGTDAEKPNFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRYKRAQGYNVLHPMGWDAFGLPAEQYAMDTGNDPADFTA
                     ENIANFKRQINVLGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLNDLEEVDWPESIKD
                     MQRNWIGKSTGANVTFKIKDTDKDFTVFTTRPDTLFGATYAVLAPEHDLVDSITSSEQ
                     EEAVAEYKRQASLKSDLARTDLAKDKTGVWTGAYAINPVNGKEIPIWIADYVLASYGT
                     GAIMAVPAHDERDWDFAKQFGLDIIPVLEGGNVEEAPYTEDGAHINSDFLDGLNKEEA
                     IAKMVAWLEENGVGQEKISYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPENELPLV
                     LPKTSDIKPSGTGESPLANLTDWLEVVREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NDEKLADEERLKAWLPVDIYIGGAEHAVLHLLYARFWHKFLYDLGVVPIKEPFQKLFN
                     QGMILGTSYRDSRGALVATDKVEKRDGSFFNIETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQFGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRIYRLIATKEISLDNSGALD
                     KVYNETVKTVTEHIEDLKFNTAIAQLMIFVNAANKEDKLYVDYAKGFVQLIAPFAPHL
                     AEELWQGLANTGQSISYVAWPTYDESKLVESEVEIVVQIKGKVKARLTVAKDLSPAEL
                     EKVALADEKVQAEIDGQTVVKVIVVANKLVNIVTK"
BASE COUNT         4704 a         3012 c         3819 g         5072 t
ORIGIN      
        1 atgagatgac agtgaaaaag aaaattgcta tgacagaaga acaattgcta catgtcgcta
       61 tgatagtcta taccttgtat gtgctgtact tacttgttac caagaatttt gtaccacgct
      121 tcataactcc ctatggactg tatctatttg ttctcgttga agaaaaatta gagaaatttt
      181 gcaaaaaact atatcacaac ttaatgaatc gataaaaaat ggtcattcat tacaaaataa
      241 cgaccgttca tgacatggat gaaaaatttg ggctgagtta gcttatacta agagtgtaaa
      301 gaaaacaaga acaggagaac tgaagatgaa gaataaaaat ttttatgcag ttttgattcc
      361 cgttgtgctt tggatagttt ttaaaatctg tgcgaatttg tcagatgaaa acattaaaga
      421 ctatttcaat attttagcag tcagtaatcc aggtctaatc atcgcttttg ccatttttac
      481 aaataaggaa caaaacaagg aaaagtaagt ttgtaaagaa aacaagaaca aggagaattg
      541 aaaatggaaa acaaacatca aagaacttag tcaagtagaa ttggaaaata ttgtaggtgg
      601 taaagtaggc attaatctcg gcggagacat tattaaagtt gccgaaaaag tatttgcagg
      661 tgtatgctca tttcttgatg gatggaataa gaaaaaatat actggtggat gttaaagtgg
      721 ggttgataga atgattaata ttttaaaaaa gtgtggctat gttttgatat ctatttttgg
      781 gtttatgttt ttatctatat ttactcaaac cccggttgtg tcaactttta gcgatgatcg
      841 tatccatgtt agtaataatc ttacaggaga tactattcga attatagtgt gtattgcttt
      901 tgtattatta tcgctttggt ttctatcatc ttttgctaaa cgactggata ttacgttgaa
      961 actactacag tttaaacaag gaaaaataaa taaaaaaagt ctagttatct actcatcagt
     1021 gacacttttt ttgacattag tatatgtgac gattctatat gttggaaata gctataatgt
     1081 aggatcagtt ccgacacaaa tactggaaat gaaggaaaga ccgatagtct ttatattgta
     1141 tcttttaacc acaatcgtta ctcagcttat tttggaagaa attttatata gaggaattct
     1201 tcaagagaaa ctatcacgtt atataggttg gggttctatt gttgttactt ctctcatatt
     1261 ttcgtatagt catgattatc aaagtgcatt taatagtaag ttaataagtt ctctaattta
     1321 tggactagct tattattcaa ctaatgatat tagaatacct agtattgcac attgtttaca
     1381 caatttcgca gtagttatgg tagttatttt gctgggataa tccgacagaa taacattatt
     1441 aatgaaaagg agaaatgaaa atggaaaaca aacatcaaat aatgcctatc aaagaaacga
     1501 ataaaattat gcagttcttg caagggcttg ttgtaggaat tttggtcact gccgttctta
     1561 ttcacctagt ggcttggctt gttcttggaa aaacagtaga tgttttctca tctgacttct
     1621 atcccactgt atttattgca ttgtattttc taatgaaagt gaatgagact aaaaaggagg
     1681 actagcttat gaaacgtgtt aaaaatattt taaaatttat tggtttgatt cttgtcatcg
     1741 ttgcgattaa cataactccg atgcgattaa ttgccataca ggattcgatg tcaggtgcca
     1801 tgcagtgggt ttctggcatt ggctatctgg tcattgcgac agctattgtg atgtggactt
     1861 ggaaacggta taagaaaggt ctacctgacc agcaaaaacg gttcacattt acttggaaag
     1921 attttgggtt tgctctgcta tttttcttgg ccggacgggt tgtcggtatt ggcggaacgt
     1981 tattgaccca gttggtaact ggtaatgcca ccacagccaa cgatgcagca cttttggcta
     2041 caaatgagca attggcgaag atgtttccgc tatactttgt tgcctttcat attgcaatag
     2101 gcgtctttgc tccttttatg gaagagcttg ttttcagagg tttgtttagc agttatttct
     2161 tcaaagaaaa tcaaaaatgg ctaaaattga taatcagttc agctgtcttt gctcttcttc
     2221 atgcgattca tccaattgaa ttgctacttt acttcttgct gggggcagtc tgctatttgg
     2281 cttatgctcg tcgtggcaat attgtggatg cgattcttgt tcatatcttg aacaatagct
     2341 tgatggttat cttctcaatt attggttact tattggtact gtttggttag tgatgttaaa
     2401 ggagttcgtt tatgacaatg atggaaaaat tagataaaaa ataccaagaa tttgaaacag
     2461 cttatgagtc ttccccgttt gcaatgaaat tcttggtctg ggtaggagta gctctgacct
     2521 tgatgacact tggatcagtt cttgtaccag cgatttattc agcagggcaa gatttcggtg
     2581 aatttctata ttctgttttt ggatgaggga gtggtagaaa ataaaatgaa aaaagctaag
     2641 caatcagctt tttgtttaca ctcgttccaa tagacttgtt gaaggtggga aagtatctcg
     2701 ttatttgagg gtttgcctga atagattgtc taggttgttt aggtagtctg tccttgctcc
     2761 tctaatgcga aggacatcga cacgagagtc acgaatcaaa tagaagatga ggtgctgttt
     2821 tccaggaatc atccttgttt ttccttctgg gaacaatgct cgtcccagac gagcgtcgag
     2881 gtcaatatat ccctctggaa atatttctaa ggtcaagata gctgtgctca gagatgcaag
     2941 cacctttcga gcacttgatt ctgaaaattc acggacatag tactggtaga tttcctctaa
     3001 atcttgttcg gctttagcag ataaatgtac ttcatagacc atatttggcg aatacctcac
     3061 gagcaggtgt tgtgtgtcct gataaaactt cttggtagcc ttcatcaagc tctgcaatca
     3121 attcatctag aaaagcctct gcacgtgcct cttctactgt ttttattggc aaatcttgct
     3181 cttccaccac cctttctaca aataaattaa gagcatcaga aagggtcata tctttttctg
     3241 ataagattat ttttgcttgt tccaataatt gtgcatttac tctaaaatta tattgtctat
     3301 ccctaaccaa tcctgccatt gtctttaact ccaaattcta atgtgtataa caatagtgta
     3361 gctatttgtt ttacatctgt caagcgataa tctggtactg aatatcttgg ataagattcg
     3421 gtgacttcga aaaatggtca ttcattacaa aatagcgacc gttcgtgaca gggatgagaa
     3481 aattggggtg agttagatta tactaaaata ggaaaaaaca attgatattc tttcgatttt
     3541 gggctggtca acttccctta attgttttat ttgttctata tatgatgaga gaggtcatat
     3601 cttttcaaat cttatggtgt gtgacaattg gaatgtgtat gtctacttct ttcgtcgtca
     3661 atcattattt tatgaaagaa gtaagaaagt agggagaact tacagtgtgg gagcctactt
     3721 tgtacttgca acagctaggt ttcatagtct tattttcaat cataggacta gtaagtttgt
     3781 atagaggttt gactgcagaa tatttctacc agaaaataat cggttttatc ggattcatca
     3841 tagcaattac ctttttaatc ttgctatgtt ggggaatccg tgctatgaaa gattggaaaa
     3901 agaataagta atacttgcaa taacatctag taaaaacagt ttaataacag tggcaggagg
     3961 gataaatggt tgattttttc agcttccatt ttgcagtgat tttatgaata gaaagctatt
     4021 attttttact ttatttttta tgaatatttc catagttgtc tattattacc tcgaaagtaa
     4081 ggtgctttct gtaaaagact ggattattat tggcacttgg ttgattattt tgagtaaaat
     4141 caataaaatg ctaaataaat ggagtgtcgt ttgacaatac attagaaaca agaaaaatta
     4201 ggaatgaaat atctgaagaa agtatgggat aagaatatga aaatgctaaa attgacaact
     4261 attattccct tgtttgtact attaatgatg ggaatactaa acttgtttcg agtgacagac
     4321 tacttgcgat atattttaaa tatactgggg ttagtaggaa tattaattct aagttatcat
     4381 acatataaaa agaacactcg gaattagttg taatagataa aaatgagtag aatatgaaaa
     4441 taataaaatc catcggttta gcagtgggta ccttgctgct cactttgctc attcaatttc
     4501 cagcttatgt ggggataaca gcctttcaag agaatgtgct actaaaactt gcagtcatga
     4561 tagggcttgg tcttccaata ttggcactac tttctgtcat cagacgagga atgatgaagc
     4621 atcaagtctt ttcaagagag acgctgaatg ttttaaaaat agtcggaatg acagcactgg
     4681 tatttactgg acaaacttgc ctgaacctct tttggcagta tttgggtctt agccttagtg
     4741 attctaacgc catggataca gtcaatcagt taaattcgga caattttggg gtgatggctc
     4801 tctcagttgt cttgtttgct ccagttttag aagaatttct ttacagagga attttgttag
     4861 aaaagacagc ccagtatttt cctaagcatc ctcaaatggt catcgtattt tctgcccttc
     4921 tatttgctta ttgtcatacc tggaactttt cagtggcatt tttaggtcac tttatcacag
     4981 gggcttatct gggctatctg tatatgtgca atagacgaat gacggatact atccttgcac
     5041 atagcttcta taatgcctcc attttattcc tccaaatttt atttggtttg gtgcagattg
     5101 gtggataaaa attgacagtt gacattttgt ggaaatgcct gtatactgag aggaagttgg
     5161 caattttata attgctaact atcaagtgca gaggtgggca gaaatatgag aaaatttttt
     5221 caagatctca atcaagaata tctggaaacc agaagttcag ttggagaaga ttggctggag
     5281 tggattatcc agagaaagat gacctggggc atgcggtggg ggttggtagg gattctcatg
     5341 tttgtcttgc aacctttaac ctataaccct cactttctat tgggctgttt ttatataggt
     5401 ttcctacttg ggttagtgta tgtatgtggg caactatttt ttgcagccag atttattttc
     5461 cgtaatgtga gaaaaaaata atcgctcagc ttgtttaggc tgggcgattg cttttttcga
     5521 tgaataattg ttgagcgatg gctgttggga cggcgagctc tttgtccttg tactggatgg
     5581 tgatggtttg ggcaaagtgg tcgaagtctg tgacggttag taaatcgccg acagctaatt
     5641 catgttgttc caagtactga aggagttgat agaagtcatg gatacggaca agctggtagt
     5701 tccctgtctc agttagctgt gacagccgtg tctggtagcg ttcgatgagc ggttgtcctg
     5761 cttgaggaat gcttcccccg tgaggacaag tttgaggctg ttccagtagc aggtctaggc
     5821 gattgataaa gtgatctgaa acggtgtgtt ctaaaatctc agcttcttca tgtacttctt
     5881 ctggagagta gccaagttgc tcaactaaga atacctcaat caagcggtgt ttgcgataga
     5941 ggctggctac catttcaagg gcagtttggc tgaggagata acctgccttg gtatccttag
     6001 aaatgagctc ttcggctacc atttttttga gcatttcgga aacggctggt gcggaaaagg
     6061 ccatcttctc tgcgatgagt ttattggtaa ttttttggtc taattgaccc agttcataaa
     6121 tacattttag gtaatcttct ttgtttggtg tcatgagaac tcgctttcta ctgagactat
     6181 tatatcaaaa aatttaaaaa tcagttgaca aaatgaattt tttatattaa tctaattata
     6241 aagttaggta tgcttaataa aaataaaagg agaacctata tgaagaaaat attgttcagt
     6301 tttgctctgc tattgtccct aatagggtta ggagcttgtc gtccatcgca aaccacagaa
     6361 ggctctagta agccaagggt ggctgtgact acttccttcc tcaatgacat ggtctatcag
     6421 ttggcaggcg atgaggtgga acgtgatttg ctgattccag caggagaaga ccctcatttg
     6481 tacgtagcca aatctagtga tttatcaaaa ttacaaaagg ctgaccttgt tctctaccac
     6541 ggtttgcact ttgaaggtaa aatggttgaa gctttggaga agaccggagt agctgtttct
     6601 aaaaacttta atgcaaaaga cttgaatacg atggatgagg atggagaaga gattgtcgat
     6661 ccgcacttct ggttttcaat tcccttgtat aagtcagctg tagctgtagc ttcagaggaa
     6721 ctacaaaaac tcttacctgc taaggctgag atgattcaga aaaatgcaga aaaataccaa
     6781 gcgcagctag atgatttgca tgcctgggta gaaaaagaat taagtgtgat tccaaaagaa
     6841 agtcgctatt tggtaacacc acatgatgcc tttaattact ttgctgctag ttatgacttc
     6901 actctttatg ctcctcaagg cgtgagcacc gactcagaag tggctaacag tgacatgatt
     6961 gagacagtta acctaattat cgaccataat atcaaggcta tctttacaga gtctacaacc
     7021 aatccagaac gcatgaagaa attgcaagag gcagtaaaag ccaaaggtgg tcaggttgaa
     7081 gttgttactg gcgaaggtaa ggagttgttc tcagattcct tggcaccaga aggcgaggaa
     7141 ggggatacct ttatcgacat gtacaaacat aatgttaaac tgatggttaa atatctgaaa
     7201 tagtacaaat ccaacggact tactattcaa tgaagactag agatattact gaagatagag
     7261 ttgataggta ccaatatgag ggataatcaa ttgtttcgag cacaaacggt ataggactct
     7321 ttctattgga ggtggtaacg cttgggggag gataggcgtt agtagcaaat cacacctgtt
     7381 attgaggtcc ttgactagaa ataccgcttg tagacctcga tgatcttggt tcacagcggg
     7441 actttatata tcagtagtct gtgtgtgaat agggatttca aatgagtagt aaaaaggact
     7501 cggtctaggt cgagttcttt ccgtttttgg accgttttta acagatttat aaacatacga
     7561 ttgaaggaag cattggtgct ctgcgaaaat caaattcatg tactgatgta ttgaaataag
     7621 cgatgtatag gggttgccag caatttgttg gtacggtatc ttaatcttat cgactttctc
     7681 agcttggtag gttggtctga ttttgatttt cattgagtat gaatgaggta tatcattttt
     7741 aaaaatagga ggaaatatgt cagcaatcat tgaattaaaa gatgtcaatt tagcatatac
     7801 agccagccag accatggctt tagaagatgt caatttggtg attcccagag ggagtcggac
     7861 ggctattgtt ggaccaaatg gtgctgggaa gtccagtcta ttcaaggtca ttttgggatt
     7921 ggaaaaacct gacacagggc aggttcgctt gcttggtcag gaggcaggtc tagagaggtt
     7981 gattgctcaa aaagtagcct atattcccca atccagtcaa gtaaattggc aatttcctgc
     8041 aacagttttt gaaattgttc tgatgggacg cttcgctcat agtaagggaa tgtttagaag
     8101 accgactaag gctgatagac agattgttga gcaggctcta gaacggttaa aaattgcgga
     8161 tttacgccat cgtcagattg atcagctttc aggtgggcag cgtcagcggg tatttttagc
     8221 gagagccctg gcacaggaag cggaattgta tcttatggat gagcctctag cagggattga
     8281 tcaggcaaca gaaatcatga taatggatat gctcaaggag ttccaatgtg aagggaaaac
     8341 ttccattgtc attcatcatg acttaacgac gctggatgcc tactttgatc accttgtctg
     8401 gcttcataag catgtgattg actctggtcc gatggatgag actttgacaa gtgagaatta
     8461 tcaggcgacc tacggcatag gaaatgggct gtttctagga aatgccaaag gaggcagtca
     8521 tgtttgaggt tttcaaagag tattctttct ggacagttgc tttagggacc gtcagcctag
     8581 ctgtagcagc cagcactatc ggaagtatat ctgttcttac taaacagagt ttgcttgggg
     8641 atgctcttgg gcatgcttct tatcctggag tgattgtctc tttcatgatt tttcagtcgc
     8701 gacatcctct ctacttgctg ttaggagcag tgttatcagg ctacctttct tatgtagttg
     8761 tccattggct gcgtcgaaaa ggtggtcaca gcctagtcaa cgccctttca ttggtgtctg
     8821 cttctttctt tggtttggga atggtcttga aaaatgctat tcagggaaat gaagcctttg
     8881 cagggtcttc ccaggctgga ttgcaaacct atctatttgg acaggcagcc tttattcagc
     8941 tggatgatgt gatactcatc ggaattattt ctctattggc tttggtattg tttgctttct
     9001 tttatcaaga ctataagctg tatctgtttg atcaaacttt tgcaagggtt attggtgttc
     9061 gtgtaaggta cttgcagcag ttgacgatgt ttttgatgat ttgcctaatt gcagtaggat
     9121 taaagttagt cggagctatt ttgatgagta gttttttgat tgcaccagct gtctttggct
     9181 tgatgctggg aaaatcgtat cataagagtc tgttactggc aggaatcgtt gcaattgggt
     9241 cggctttcgt agggacctgg attagctcca gtgtatctgg tttgtcgacc ggaccgacta
     9301 ttatcgtctg tctgacaggt ctgaccctgt ctgcatttgt gtatgttacc tatgtcagaa
     9361 aggagaatgg tcgtgtttga agtattactc attttgatgg ttattgctag ttcttgtggc
     9421 ttgctcggct cgatactggt tgtaaaaaat caatcgatgt tggcagatgc gctatcgcat
     9481 tctgtcttgc ttgggattgt tctgggattt tttattagtc atagtttgga ttcgccattg
     9541 ctaatagttg gtgccagtct gtttgggtta ttatcggttc ttgccattga ccgtctgcat
     9601 agtcgaaaga tagcgcatga tgccgcaaca ggtctagttt tttctttctt tttcgcagtg
     9661 gcggttttac ttatttctct ttttgctcgc aatgttcatt tggatgtgga tctggttttg
     9721 cagggggaag tactgtttgc tccgctccat cgtatggatg ttctagcctg gtcactccct
     9781 gttagtctgg tcaagtcgag tttggcttgg ttggttattg ttctattttt tgcttggggt
     9841 taccatcgtt tacaagtgta cctatttgac agcaaccatg ctcgtttgtc tggcttacga
     9901 acaaggattt tagaaatggt tattttgatt ctagtttcct taactactgt gctggccttc
     9961 gaagcgattg gctcgatgac tgttattgtt ttcttggtgg caccaagtat ggctgcccta
    10021 cgttgggtca aatccttttg gcaattgctt cttttagggc aaggtatcgc tattctaacg
    10081 gttgcgctag gttttttagt ggccaatcaa ctggatttaa ccatgtcagg gacctgtgca
    10141 gttgtcagcc ttcttgtggt ttgttcgagt attattctaa aaaatacttg gtcacggtca
    10201 tctgataagt gaaattgtca gaattttttg ttatcataga caatatgtaa agacccccag
    10261 ttgagaattc gtggatttac ctgtacacta gaataaaaaa caatcaactt ctaacaggaa
    10321 ttaccacact caattggagg tcttatatgt ttcattttac cacacttttc atcggaatgg
    10381 atgttcacaa agaaagtttt tcactctgct attatgatat gatggcgaat caattcaaac
    10441 atagcactaa agttggtcca aatgttagct atattgtgaa ctatgtgaat gaggttcgtg
    10501 gtttatatgg tcaatatgca gaagtgttat gtggctacga agcccggatg tcttggattt
    10561 accctatatc accagctaca agctcacggg cttccctgta tcgtgatggc gcctacaacg
    10621 gtgatgaagg aaggatctaa gcgtgttaag actgataaaa aagatgcagc tcagctcgca
    10681 aaagctctgg cctttcgtag ctatcggtct gttcatattc ctactgttga ggatgaacaa
    10741 gtcaaagaat atatccgcat gagaacagac cacaaagtgg ctctgaagaa aatcaaacaa
    10801 caaattcttg ccttctgtct ccgacatgat tttcgctata ccgagggaag aaataattgg
    10861 acatagaaac atgtctgctg gttccattcc ctaaatccta agggacttta cgcagagatt
    10921 ttgacagaat acctattgac ctatgagaaa ttagtagatc aaatagaacg gtatgatgcg
    10981 cgaattgagc aactgggtca aagcgacagt tatcaagaga aggtctcacg gctttcttgc
    11041 tttattggca ttaaaacact aaccgctctt tccattgtga cggaaatcgg tgattttaac
    11101 cgctttgcga cagctcaaca ttttgcttct tatcttaggc taactcctag cgaaaattct
    11161 agtggcgaca aggagagaag aggcgctatc accaaagctg ttaatagcca tgtaagacgt
    11221 cttctgatag aagctgcaca atcattggct aaggggacga ttgggtataa atccaaagaa
    11281 ttgaaaagga gacaaagtgg aaaccgagtg gaggtgattg cttatgcgga taaggctaat
    11341 gaacgcttaa gaagacgtta tcgcacactt gttctaggaa aaaataagaa acaaaatgtt
    11401 gctaaaacag ctattgcacg agaattatct ggttttattt ggggatgatg acaggaagaa
    11461 tagcttgaag ttagcgcttg ttttcatgta cacttttgac aattaagttt tatcatcgca
    11521 gagcgatgag gaatccctat ttctatccca aatcactggg tgaatttgga cagaaataag
    11581 gattgaaata atgtttgaat ggagagtatc ttgaaggagt ttaggacttc aaggtttgag
    11641 tcatctacga aatgactaag tagcacaatt gtgatcatgc ttatagagag taggactcgc
    11701 ggcagaacca ttgacctgta ggtaaccaat ctacgaatat cagagtggcc aatgcccgaa
    11761 gctaagaact aggctctttc tagatacttt tcatttttta tcagttcgat tgtttttact
    11821 tgacaaaggg ctttacatat cagtcattaa gaaagacaaa ggagaaaatc aatgacacgt
    11881 ttacaagatg atttttacga atacgtcaat ggtgaatggg caaaaacagc cgtgattcca
    11941 gacgataagc caagaacagg tggtttcacg gacttggcgg atgagattga gaagctcatg
    12001 attgcgacaa cgaatggctg gttggctggg ggaaatgtcc cagaagatag cgttttgcaa
    12061 aactttgtag ccttccacaa gcaggtggct gactatgaga ctcgtaatcg tttgggagca
    12121 gagcctgtcc aggctttgat tgcggagtac aaggccttga attcttttga ggaatttacc
    12181 agcaagttgg cagagtttga gttggcaggt aagcccaact tcatgccttt tggggtgtcg
    12241 cctgacttta tggatgccca gaccaatgtt ctttgggcct atggcatagc gaccattttg
    12301 ccggatacga cctactatgc tgacgggcat gagaagggag cggagttact taagacttgg
    12361 agggagtgtc aggaggctct cttgcctaag tttggtttca gcgatgagga aattgcagag
    12421 ctcttggata aggtcttagc cttggatgca attgtggcaa aatatgtcct gtcaaacgaa
    12481 gagggatcag agtatgccaa actctatcat ccataccaat gggaagactt tgcagccttg
    12541 gcaccaaacc tgcctttgac agctttcttt gaggaacttt taggacaggt gcctgataag
    12601 attatcgttc cagaggagcg tttctggcag gcggctaatg agatttacag tcctgaaaac
    12661 tgggacttgc tcaaggctgg tttggtattt gcagcgactc gttcttatac ttctttccta
    12721 tcagacgaga ttcggatttt ggacggagct tattctcgtg ccctttctgg tacgccacag
    12781 gctcagaata gggaaaaggc agcctacaac cttgctcaag gtttctttag tcaggccttg
    12841 gggctctggt atgcagggga aaaattctca ccagaggcta aggcggatgt ggaaaccaag
    12901 gttgctaaga tgattgaggt gtacaaatct cgcttggaaa cggcagattg gttggctcag
    12961 gaaacgcgtg acaaggctat taccaagctc aatgtcatca agccttacat tggctatcca
    13021 gaagaattgc cagctcgtta ccaggacaag attgtcgatc caagcaagtc cttggtggaa
    13081 aatgctgttg aattgattaa ggtggatatt cgctatgctt ggagcaagtg gaatcaaccg
    13141 gtcaatatca aggagtgggg catgccagcc cacatggtca atgcctacta caatccacag
    13201 aagaacttga ttgtctttcc agcggcgatt ttgcaggctc cgttttactc cttggagcaa
    13261 tcttcgtcag ctaactacgg tggaatcggt gcggttattg cccatgagat ttcccatgcc
    13321 tttgactcca atggtgcttc ctttgacgag catggtagtc ttaataactg gtggacagag
    13381 gaagactatg cagcctttga ggcccgtacc cagcaggtta tcgaccagtt tgagggtcag
    13441 gattcttacg gtgccaaaat caatggtaaa ttgaccgtgt cagaaaacat tgctgacctt
    13501 ggtgggattg cggcagccct agaagcagcc aagtcagaag atgacttctc cgcagaggaa
    13561 ttcttcacta actttgcccg tatctggcga atgaaggctc gtcctgagtt catgcagatg
    13621 ttagctagtg tcgatgtcca tgcaccaggt cacctacgca ccaacatcca gttgccaaac
    13681 ttcgatgaat tccatgaaac ctttggcgtt caagaaggcg acggcatgtg gcgtgcaaaa
    13741 gaagaccgcg tgattatttg gtaaaaatgg gaaaacagct agtgaaaact ggctgttttt
    13801 gtctttccca attcccccaa aacgtgatat aatagtaaag ataatttatt gacatggaga
    13861 aatgatatga gcttctacaa tcacaaggaa atcgagccca aatggcagga attttgggca
    13921 aaaaatcata cttttaagac aggaacagat gcagaaaagc caaactttta tgccctagat
    13981 atgttccctt atccgtctgg tgcgggcttg cacgttggtc accctgaggg ctatacggcg
    14041 acggacattc tcagccgtta caagcgtgcc caaggctaca acgtccttca cccaatgggt
    14101 tgggatgcct ttggtctgcc agcagagcaa tacgctatgg acacgggaaa tgacccagct
    14161 gactttacag cagaaaacat tgccaacttc aaacgtcaga ttaacgtcct tggcttttct
    14221 tacgactggg accgcgaggt taatacgact gaccccaact actacaagtg gacccagtgg
    14281 attttcacca agttgtatga aaaaggccta gcctatgagg cagaagtgcc tgtaaactgg
    14341 gtagaggaat tgggaactgc tatcgccaac gaagaagtcc ttcctgacgg aacgtctgaa
    14401 cgtggtggct acccagttgt tcgcaagcca atgcgtcaat ggatgctgaa aatcactgcc
    14461 tatgcagaac gcttgctcaa tgatctagaa gaggttgatt ggccagagtc tatcaaggac
    14521 atgcaacgca actggatcgg caagtcaacc ggtgccaatg tgactttcaa aattaaggac
    14581 acagacaagg acttcacagt ctttactacc cgtccagata ctctttttgg tgcgacctac
    14641 gcagtccttg cccctgagca cgacttggtg gatagcatta cctcatcaga gcaggaagaa
    14701 gctgtggcag agtacaaacg ccaagcctct cttaaatcag accttgcccg tacagacctt
    14761 gccaaagaca agacaggtgt ctggacaggt gcttatgcca tcaaccctgt caacggtaaa
    14821 gaaattccaa tctggattgc cgactatgta ctggcaagct acggaacagg tgccatcatg
    14881 gcggttcctg ctcatgatga gcgtgactgg gactttgcca aacagtttgg tttggacatc
    14941 attccagttc tggaaggtgg caatgtagaa gaagcacctt atacagagga cggtgcccat
    15001 atcaattctg acttcctaga tggtcttaac aaggaagaag ctattgccaa aatggtggct
    15061 tggttggaag aaaatggtgt cggtcaagag aaaatttctt accgcctccg tgactggctc
    15121 ttcagccgtc agcgttactg gggtgagcca attccaatca tccactggga agacggtact
    15181 tccacagcag taccagaaaa tgagttacct ctggtattgc caaaaacctc agacatcaag
    15241 ccttcaggta caggggagtc acctcttgct aacttgacag actggttgga agtggtgcgg
    15301 gaagacggcg taaaaggtcg ccgtgagaca aacaccatgc cacaatgggc gggttctagc
    15361 tggtactacc tccgctacat cgacccacac aacgatgaga aattggcgga tgaggagcgt
    15421 ctcaaagctt ggttgccagt ggacatctac atcggtggtg ccgagcacgc agtccttcac
    15481 ctgctctacg ctcgcttctg gcacaagttc ctctatgacc ttggcgttgt gccgatcaaa
    15541 gagccattcc aaaaactctt taaccagggc atgattttgg gaactagcta ccgtgacagc
    15601 cgtggtgccc ttgtggcgac tgacaaggtg gaaaaacgcg acggttcctt cttcaacatc
    15661 gaaactggtg aggagttgga gcaggcacct gctaagatgt ctaagtctct taaaaacgtg
    15721 gttaacccag atgatgtggt tgagcagttc ggtgcggata ccctccgtgt ttacgagatg
    15781 ttcatgggcc cacttgatgc ctctatcgct tggtcagaag aaggccttga aggtagccgt
    15841 aagttccttg accgcattta tcgcctgatt gcaacgaagg aaatttcttt ggacaatagc
    15901 ggagcgcttg ataaagttta caatgaaact gtgaagacgg tgacagagca tattgaagac
    15961 ctgaaattca acacggctat cgcccagctc atgatttttg tcaacgcagc caacaaagag
    16021 gacaagctct atgttgacta cgccaaaggc tttgttcaat taatcgcccc atttgcacca
    16081 cacttggcag aagaactctg gcagggtctg gcaaatacag gtcagtcaat cagctatgtc
    16141 gcatggccaa catatgacga aagcaagcta gtagaaagcg aagtggaaat cgttgtccaa
    16201 attaagggca aagtaaaggc tcgcttgact gtagctaaag acctgtcacc agctgaactt
    16261 gaaaaagttg cccttgcaga tgaaaaagtc caagctgaaa tcgatggtca aacagtggtg
    16321 aaggtgattg ttgttgcaaa taaactcgtc aatatcgtta ccaagtaatg attgcctcgt
    16381 ttgtttgttt atctccaatc tccaacagtc tatccgcaga ctgttggagc caactaaact
    16441 tgttaatatt gttgtgaaat aaatgtaaat aataagaaaa cgacttccgt attggaggtc
    16501 gtttttgtat tttctagaat gatcatgttg tcttaatttc cgttgtgata cggaaaaaga
    16561 gggtctgtgt atccgtttac aatcgatgtg aactgaaata taataag
//
LOCUS       DATUCG010000048        16397 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.049, whole genome shotgun sequence.
ACCESSION   DATUCG010000048 DATUCG010000000
VERSION     DATUCG010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16397
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.049"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(145..1944)
                     /locus_tag="VCA20_001186"
     CDS             complement(145..1944)
                     /locus_tag="VCA20_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEP1835017.1"
                     /translation="MKIKWLSVIRVIGLVFVLLYHFFIKYFPGGFVGVDLFFTLSGYL
                     TTALLIDEFAKHKKIDIVSFFRRRFYRILPPLVLTILLVLPLALLVRNDFIANIGNQI
                     AAALGFMTNFFEILSGGSYENQFSPHLFVHTWTLAIEVQYYLVWAGLVWYLTRISKSV
                     GQLRGSIFLTSISLFSLSFLAMSISSFFVDNFSTIYYSSFTHTFPFFLGSILATIAGI
                     GNTTTAFQQQVNTLSIKQAIGLFLGGLGVELLLLFTLQFSSIWTYLIGFLLSSLATAT
                     MIFAARVLHEKTEDVKEPAIILFFSSISYSIYLFHWPLLIIFTQLTNATIAAGLSFVF
                     STILSTISFYILEPYIAGKVGSLFGLAIDLKPYKKWLLSSFACLTIVGLGICLFAPKL
                     GSFEQESMVSNLYQAQTQLNTTRSAAENAKATSFEIQKGVIIFGDSVTVRASAAIQTA
                     LPDAQIDGTVSRNLSEIAKLINLYKQNNSLKETVVVALGTNTTDNYQELLDQLVKDFP
                     KGRRLIFVTPYDGNFTPSESISYQTGQYEKELAEKYDYISIADWYQVAKDNPPIWYNT
                     DLVHFNLESNGGEVFAQTIKNAVEAVADGPIKN"
     gene            complement(1934..2356)
                     /locus_tag="VCA20_001187"
     CDS             complement(1934..2356)
                     /locus_tag="VCA20_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835018.1"
                     /translation="MKEFKLPSIKWHDITSHFTRPKLEILSLIIILICALSVFTGRIA
                     SKQAMTFNNGALQYNGYVVTNKMNGQGKLTFDNGDVYEGQFTNGIFHGQGTYTSASGW
                     VYTGQFKNGYADGKGKLTTEGQATYEGTFKQGIYQYEN"
     gene            complement(2488..2922)
                     /locus_tag="VCA20_001188"
     CDS             complement(2488..2922)
                     /locus_tag="VCA20_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEP1835019.1"
                     /translation="MKKIVFVCLGNICRSPMAEFVMKDLTNQFHIESRATSNWEHGNP
                     IYPGTQKIFQKHKIPYDKTKISQQISQADFEEFDVIIGMDSNNVRDLQKMAPAHAQNK
                     IFQFAEKSLPDPWYTGDFDETYEMVKKGCQDWLDRLQSLGQE"
     gene            complement(3092..3304)
                     /locus_tag="VCA20_001189"
     CDS             complement(3092..3304)
                     /locus_tag="VCA20_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEP1835020.1"
                     /translation="MSEEKFDAKLEQLSGSAKQVIGKLTGDKEIEVEGLVEKGIGKAK
                     ELVEDAKDRIEGATNGIKNAFDKEEK"
     gene            3505..4023
                     /locus_tag="VCA20_001190"
     CDS             3505..4023
                     /locus_tag="VCA20_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEP1835021.1"
                     /translation="MMKQKYYQSPAEVASFSPRPSLADSKAVLNQAVADLSVAHSILH
                     QVHWYMRGRGFMIWHPKMDEYMEEIDGYLDEMSERLITLGGAPFSTLKEFSENSQLKE
                     VPGDYNVTIEEQLARVVEVFRYLAALFQKGFDVSDEEGDSVTNDIFNVAKASIEKHIW
                     MLQAELGQAPKL"
     gene            4271..5149
                     /locus_tag="VCA20_001191"
     CDS             4271..5149
                     /locus_tag="VCA20_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1835022.1"
                     /translation="MIEVRALDKVIKGRTILSNISFEIKSGECVALIGPNGAGKTTLM
                     SCLLGDRKAMKGQVLLDGLAPQARSNKETVAVLPQENAIPTDLKVKELLRFFQAIYKD
                     SLTDVEIDSLLCFSPEQKNQLAGKLSGGQKRLLAFVICLIGKPKLLFLDEPTAGMDTS
                     TRQRFWEIVHELKAQGVTIFYTSHYIEEVEHTAERILVLHQGRLLRDTTPFAMRSEEQ
                     EKEVTLPIAFAAAVESLAEINEISYKQDTVSFKTRAIEQVWASLQEAGCRISDLEVQN
                     KTLLNSLFDKTREEES"
     gene            5146..5871
                     /locus_tag="VCA20_001192"
     CDS             5146..5871
                     /locus_tag="VCA20_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP1835023.1"
                     /translation="MKALVQVEAVKLSRSLGVFLLSIGMPVIFFLIFSSTVQFDDAAM
                     QKAFIQSYMLTMTGFSMSGFALFTFPMMLAEDRKNSWLTFIQHSPLPIWHYYLSKLVR
                     VLLCFVSSITIVFAVGGLVKGVDLSAQEWVVSALILLVTSIVFLAIGLLLVQIQSEQA
                     MSVVANLLYFVLAIMGGSWMPVSLFPDWVQRICKLLPSYHVNQLVTTYAQKGDFLWKS
                     LLIVLGYAIIFLGIALVLNRKSE"
     gene            5886..6980
                     /locus_tag="VCA20_001193"
     CDS             5886..6980
                     /locus_tag="VCA20_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEP1835024.1"
                     /translation="MIFEKRKVPLMFFVGMVFLYFPFYYAQYSPNPTAVVLATILFAL
                     VYCSLFYTDHKLYSLLGWFYLIGYIAVMSFWGNLQFSLFIYNLSNMLGWYYKETRPTY
                     RTVSYGILLLAIILWALFGPVPFEMRAFALVIHFFGLALLIFSWIETKREALQQRLQE
                     QNASINLLLAENERNRIGQDLHDTLGHVFAMLSVKAELVQTLLDHDQIDQAKKEVADL
                     QTLAKDSMQEVRQIVQSLKQHTVAEELQILQQMLNLAGVDLVVLGGEIAEQLSLPAQN
                     KLAMVLRELANNLLKHSQASKCRLVFEKDQQELVLHYEDNGVGFTQLTGQELHTIKDR
                     LVTVKGSLEFLSLAKPTRLSIRIPFEEVVE"
     gene            6977..7579
                     /locus_tag="VCA20_001194"
     CDS             6977..7579
                     /locus_tag="VCA20_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEP1835025.1"
                     /translation="MRILVAEDQALLRDALCQLLGFQDAVEAVLQVENGSQAIALLEK
                     ESVDVVLLDIEMPAKTGLDVLEWARGQALPVKVVIMTTFKRPGYFERAVKADVDAYVL
                     KERSIADLMRTIETVLAGRKEYSPELMDILLTQRSPLTNLESQLLKLVADGLSNKEIA
                     SQLHLSDGTVRNYMTSILSKLGAENRTAAVKVAQEKGWLV"
     gene            7887..8105
                     /locus_tag="VCA20_001195"
     CDS             7887..8105
                     /locus_tag="VCA20_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEP1835026.1"
                     /translation="MKRLYDVQQLLKRFGIIVYMGNRLYDIEMMQIELNRIYQAGVLD
                     RLEYMEAELVLRREHRLELEYQKSRENE"
     gene            8105..8491
                     /locus_tag="VCA20_001196"
     CDS             8105..8491
                     /locus_tag="VCA20_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEP1835027.1"
                     /translation="METFWVFLVFLAVLGAWMGFNYWRLRRAATVIDNAEFAEKIHGG
                     QLIDLRDPSEFNRKHILGARNIPYQQLRQSTAALRKDRPVLIYENDRGQLVTPAALHL
                     KKQGFNEIYILSYGLNGWNGKVKTTK"
     gene            8548..9270
                     /locus_tag="VCA20_001197"
     CDS             8548..9270
                     /locus_tag="VCA20_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEP1835028.1"
                     /translation="MEKKFEKLSLAEIGIISFMGLILLISLGASWYKNAQSETSDTKT
                     VQQVPNQEKTVDTLFQDAENAVIVLEGTHTEEKLAEAQSAVNKVTDATKKATLQKRID
                     QVKELVGQQTDAQTQAKLKAELVRVEAAQKVADEKKVSETGTSQSTDLAEASETLQES
                     YVVPNQNIYGNQSVSSTSQYTSSVASTSTSTSVPPTSEGAPVVETPTVSETPAETTDS
                     TTSLVESVETVPTADESSPDNP"
     gene            9579..10118
                     /gene="rsmD"
                     /locus_tag="VCA20_001198"
     CDS             9579..10118
                     /gene="rsmD"
                     /locus_tag="VCA20_001198"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEP1835029.1"
                     /translation="MRIVSGSYGGRPLKTLDGKTTRPTSDKVRGAMFNMIGPYFEGGR
                     VLDLYAGSGGLSIEAISRGMESAVLVERDRRAQAIIRENIAMTKEADKFQLLAMEARQ
                     ALNSVGGPFDLVFLDPPYAEQEIEDVITELCRRDLIADDVMVVCETDKAVSLPEEIAE
                     LGIWKEKLYGISKVTVYVR"
     gene            10108..10596
                     /gene="coaD"
                     /locus_tag="VCA20_001199"
     CDS             10108..10596
                     /gene="coaD"
                     /locus_tag="VCA20_001199"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEP1835030.1"
                     /translation="MSDKIGLFTGSFDPITNGHLDLIERACGLFDKLYVGVFTNPKKA
                     GLLTGLERKAILEKLFVGMENIEVVLSENELVVDVAKRYGVTHLVRGLRNATDLEYES
                     SFDFYNRQLAPGLETIYLIAKPELKFVSSSQVRELLYFKQDIDPYVPEIVSEEIRKNE
                     EK"
     gene            10583..11632
                     /locus_tag="VCA20_001200"
     CDS             10583..11632
                     /locus_tag="VCA20_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEP1835031.1"
                     /translation="MKKNRKLTLIASIVLGTLLIWFAVFVRLPYYLESPGGAADIRQV
                     LTVNNQIDQEDGSYNFTYVSVQQATALQLFAAQFDPYTTVRSSEEMTGGADNEEYFRI
                     ARFYMETSQNMAKYQGLTLAGKEVNLDFFGVYVLALTDDSTFKKVLNIADTVVSINGK
                     TFESSPDLIKYVSGLEVGSDVTVGYISAGQEKSADGKIIKLSNGKNGIGITLVDHTEV
                     QSSVPIDFQTGDIGGPSAGLMFTLAIYTQLAEPDLRDGRIIAGTGTIEQDGKVGDIGG
                     ADKKVISAAKSGASIFFVPNNPVDVEVLKKNPKAKTNYEEAKEAAEKAGLDIEVVPVK
                     TVQEAIDYLKKTKGE"
     gene            11820..12461
                     /locus_tag="VCA20_001201"
     CDS             11820..12461
                     /locus_tag="VCA20_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1027 domain-containing protein"
                     /protein_id="HEP1835032.1"
                     /translation="MKKEISPEMYNYNKYPGPSFARVGDKVVSENIELDLLEDYKNAF
                     DQTIFGQRFSQLMLKFDYIVGDWGNEQLRLRGFYTDDKNVKSDLKISRLDDYLTEYCN
                     FGCAYFVLANPNPQDLPAEEEERPRRKRSRSRNRNRNQQRTEPIAPKENKSNRNDRQK
                     KGKQQNKQSEQRHFTMKNAGVKASQTERSQKRERKQSKREMNEVKRNFVIRQK"
     gene            12470..13549
                     /gene="rlmN"
                     /locus_tag="VCA20_001202"
     CDS             12470..13549
                     /gene="rlmN"
                     /locus_tag="VCA20_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEP1835033.1"
                     /translation="MKPSIYAFSQANLVDWILENGEKKFRATQIWEWLYRSRVQSFAE
                     MTNLPKSLIEKLEEHFVVNPLKQRIVQESKDGTIKYLFELPDGMLIETVLMHQHYGLS
                     VCVTTQVGCNIGCTFCASGLIPKQRDLTSGEIVAQIMLVQKYLDERNQNERVSHIVVM
                     GIGEPLDNYDNVMTFLRVVNDDKGLAIGARHITVSTSGLAPKIREFAREGVQVNLAVS
                     LHAPNNDLRSSIMRINRKFPIEVLFEAIEDYIKVTNRRVTFEYIMLNEVNDGVEQAQE
                     LADLTKNIRKLSYINLIPYNPVSEHDQYSRSTKERTLAFFDVLKKMELTVSFVRNMVQ
                     ILMLPVVNCAPIRSRKTVKKPGLVL"
     gene            13578..14105
                     /locus_tag="VCA20_001203"
     CDS             13578..14105
                     /locus_tag="VCA20_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEP1835034.1"
                     /translation="MRKFFQADGNLTNLGRQICKTLAGAYALAIVLLCFLPQTWYPQY
                     KDFSTPGIIQLGRLYLLPTPFNSIVNGDKVDSLADLGWIFLQNITNIFLLFPLIFLLL
                     FLREEWRSLRAVIRYSFCISLFIECTQLLLDLLIDAQRVFEIDDLWTNTMGGILAYFT
                     YSLIKRGIILSKQES"
     gene            14924..16096
                     /gene="trpB"
                     /locus_tag="VCA20_001204"
                     /pseudo
     CDS             14924..16096
                     /gene="trpB"
                     /locus_tag="VCA20_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942848.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
     gene            16289..16397
                     /locus_tag="VCA20_001205"
                     /pseudo
     CDS             16289..16397
                     /locus_tag="VCA20_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005576373.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT         4735 a         3060 c         3815 g         4787 t
ORIGIN      
        1 tttttgtttc atttagctat atttcacaag atttattgag actgagttca ggcacagcgc
       61 catttcttag agtttgcgct ggacttatag gaaaatagta caaaaatcct ccgagaaatt
      121 ctcagaggat gatgatgtat aaatctagtt tttgattggg ccatcagcaa cggcttcaac
      181 agcatttttt atcgtttggg cgaagacttc gcctccattg ctttcaagat tgaaatgaac
      241 aaggtcggta ttgtaccaaa ttggtggatt atcctttgca acttgatacc agtctgcaat
      301 ggaaatgtaa tcatattttt cagccaattc tttctcgtat tgaccagttt gataggaaat
      361 ggattcgctc ggagtgaagt ttccatcgta aggagtgaca aaaataaggc gacgtccttt
      421 aggaaaatct ttgactagct ggtctagtag ttcctgataa ttatccgtag tatttgtacc
      481 tagggctaca actacagtct ctttcaagct attattttgc ttataaagat ttatcaattt
      541 agcaatttca gagagatttc gactgacagt tccatcgatc tgtgcatctg gtagcgcggt
      601 ctgtatggca gcacttgcgc gaacagtaac agaatcgcca aagatgataa ctcctttttg
      661 aatttcaaaa ctagtagctt tagcgttttc agcagcagat cgtgttgtat ttagttgggt
      721 ctgagcttga taaagattgc taaccatgct ctcttgttca aaacttccta attttggcgc
      781 aaacaggcag atacctaagc caactatggt caagcaagca aagctgctaa gaagccattt
      841 tttatagggt tttaagtcta ttgctaatcc aaacaagctt cctactttac cagcaatata
      901 tggttctaga atatagaagg aaatagttga taggatagta gaaaaaacaa aggataatcc
      961 cgcagcgata gtggcatttg tcagttgtgt aaagataatg agcaagggcc aatggaagag
     1021 gtagatacta taagaaatac ttgaaaagaa gaggataatt gcaggttctt tgacatcttc
     1081 ggttttctcg tgaagtaccc gagcagcaaa aatcatagtt gctgtagcta aactggatag
     1141 aagaaaacca attagatagg tccagatgga actgaattga agagtaaaga gaagaagaag
     1201 ttctactcct agtcctccaa gaaacagacc tatagcttgc ttgatactca atgtattcac
     1261 ttgttgctgg aaggcagttg ttgtatttcc gataccagcg atggttgcga gaatgctacc
     1321 gaggaagaat gggaaggtat gtgtgaagct cgagtagtaa atggttgaga aattatccac
     1381 aaagaaactt gagatagaca ttgctaaaaa acttagcgag aagaggctta tagatgttag
     1441 aaaaattgat ccacgtagtt gccccacact ttttgagatg cgagtcagat accaaactaa
     1501 accagcccag acaaggtaat actgaacttc tatggctagt gtccaagtat gtacaaatag
     1561 atgaggtgaa aattgattct cgtaactacc acctgataga atttcaaaga agtttgtcat
     1621 gaatccgagc gcagcggcta tctgattgcc gatattggca atgaaatcat tgcggacaag
     1681 cagagcaagt ggaagaacca aaagaatcgt taaaaccaga ggggggagaa ttcgatagaa
     1741 ccttcttctg aaaaagctga caatatctat ttttttatgt tttgcaaact catcaatcaa
     1801 aagggcggtt gtcaaatatc cagacagggt aaagaagagg tcaaccccaa caaagccacc
     1861 tgggaaatat ttgataaaga aatgatagag taggacaaag actaatccaa tcacacgtat
     1921 aacggataac catttaattt tcatattgat aaatcccctg tttaaaagtt ccttcgtagg
     1981 tagcttgtcc ttcggtggtc aattttcctt ttccatcggc gtagccgttt ttaaattgcc
     2041 cggtataaac ccacccactt gcagatgtgt aggttccttg accgtggaaa attccatttg
     2101 taaattgtcc ttcgtagaca tctccattat caaaggtcaa ttttccttga ccattcattt
     2161 tatttgtgac cacataaccg ttgtactgca gggcgccatt attgaaagtc atggcttgct
     2221 tgctggcaat tcgtcctgta aatacagaga gggcacagat gaggatgata ataagggaga
     2281 ggatttcaag ttttggacgg gtgaaatgac ttgttatatc atgccatttt atgctaggta
     2341 atttgaactc tttcatcgtt ttactctttc ataggatttg accgatgcta atcagttctt
     2401 tctaggatac agtaagaaat tatcagttca gccagaaata gtttaccatt tttttacaaa
     2461 tattacaaaa atattacata gaaaatctta ttcttggcca agtgactgta acctgtccaa
     2521 ccagtcttgg caacctttct ttaccatttc gtaggtttcg tcaaaatctc cagtatacca
     2581 agggtctggt aaagattttt ctgcgaattg aaaaatctta ttttgggcat gtgctggtgc
     2641 cattttctgt aaatctcgga cattattgct gtccataccg atgataacat caaattcctc
     2701 aaagtctgct tgagagattt gttgagagat tttggtcttg tcatagggaa tcttgtgctt
     2761 ttggaaaatt ttctgtgttc cagggtagat aggattacca tgctcccagt ttgaagtggc
     2821 acggctttca atatgaaatt gatttgtcaa atctttcatg acaaattcag ccataggact
     2881 acgacaaata tttccaagac agacaaaaac gatttttttc atagattctc ctttcgtggc
     2941 ataagaaaag agaaggtaga ctcgacaggt aaatgtctga gttcccctct cttgtatatt
     3001 tttgttcggg ctagaggtaa aagaccaaat taagtgtaat gttcattatc aaacagtgca
     3061 cgagcaatcc tacctatatt gaactctttg tttatttctc ttctttgtcg aaagcatttt
     3121 tgatgccgtt agtggcacct tcaatcctat cttttgcatc ttcgactagc tcttttgctt
     3181 tgccaattcc tttctcaaca agtccttcaa cttcgatttc cttgtcgccg gtcagcttac
     3241 caattacctg ttttgcagag ccagatagtt gttctagttt tgcgtcaaat ttttcttctg
     3301 acataaaatc cctccttgat atacacatat ggggcgatga ttttctaaaa agaaaatcac
     3361 atactattct tacttactag tctatcgtat ataagttaat atagcaaggg atttgtctgc
     3421 caataattca gaggaggagg gataattctt gcaatttttt cttcttttat ttataatatt
     3481 tataaataaa agaaaggatg atatgtgatg aaacaaaaat attatcaatc tccagcagaa
     3541 gttgcgtctt tcagcccgcg tccatcattg gctgattcta aggctgtttt gaatcaagcg
     3601 gtggccgatt tatcagtagc ccattctatc ctccatcaag ttcactggta tatgcgtggt
     3661 cgtggtttta tgatttggca tccaaagatg gatgaatata tggaagaaat tgatggctat
     3721 ttggatgaga tgagtgagcg tttaatcacc ttaggtgggg caccattttc tacccttaaa
     3781 gagtttagtg aaaatagtca gctcaaggaa gttcctggtg attacaatgt aacgattgaa
     3841 gagcaattgg cgcgtgtggt agaggtgttc cgctatctgg ctgctctttt ccaaaaggga
     3901 tttgatgtca gtgatgaaga aggtgacagc gtaaccaacg acattttcaa tgtggctaag
     3961 gctagtattg aaaaacatat ttggatgttg caggcagaac ttggtcaagc acctaagttg
     4021 taaatagatg aagggtccac agtaggactc ttttttgctg cattatttaa tactcttcga
     4081 aaatcaaaat taaccgttgt caacttgaac tctagattta tcttcgactt cgtttcctag
     4141 gctacttttg attttcattg agtataatat gacatttgtc acatgagtca atgacaaagg
     4201 cttctggtag ggtctccttt ttcgaggtat actatgtatg taagatgttc aagaagtagg
     4261 aggtagaaaa atgatagaag tacgggcact agacaaggtt atcaaaggtc ggacaatctt
     4321 gtccaatatt tcctttgaaa tcaagtcagg agaatgtgtg gccttgattg gtcccaacgg
     4381 agctggaaaa actaccctca tgtcctgttt gttgggtgat agaaaggcga tgaaaggcca
     4441 ggttttgttg gacggtctgg ctccacaggc tcggtccaat aaagaaacgg tagcagtctt
     4501 accacaagaa aatgccattc cgacagactt gaaggtcaag gaattgctcc gctttttcca
     4561 agccatctac aaggatagtc tgacagatgt ggaaatcgac agcctgctct gcttttcccc
     4621 agagcaaaaa aatcaattgg ccggcaagct atcgggtggt cagaagcggc tcttggcctt
     4681 cgtcatctgc ctgattggca agcccaagtt gctcttcttg gacgagccga cggcgggcat
     4741 ggatacgtcg acccgccagc gtttttggga gattgtccat gaacttaagg ctcagggtgt
     4801 gactattttt tataccagcc actatatcga ggaagtggag catacggcgg agcggatttt
     4861 agtcttacat caaggccgac tcttgcgaga taccacgcca tttgccatgc ggagcgagga
     4921 gcaggagaag gaagtaaccc tgccgattgc ctttgcggcg gcggtggaga gcttggctga
     4981 aatcaatgaa atcagctata aacaagacac ggtcagcttt aagactcgag ccattgagca
     5041 ggtttgggct agcttgcagg aggcaggttg tcggatttca gacctggaag tgcagaataa
     5101 gaccctgctc aacagcctat ttgacaagac gagggaggaa gaatcatgaa agcattagtt
     5161 caagtagagg cggtaaaatt atccagaagt ttgggagtat ttctcctgtc tatcggcatg
     5221 cccgtcatct ttttcctgat cttttcatca acggtccagt ttgatgatgc agccatgcaa
     5281 aaggccttta tccagtccta catgctgacc atgacaggct tttccatgtc gggctttgcc
     5341 cttttcacct ttcccatgat gctggcagag gacagaaaga atagctggtt aacctttatc
     5401 cagcattcgc ccctgccaat ctggcactac tacctgtcca aactggtgcg ggtcttgctc
     5461 tgctttgtat cttccattac cattgtcttt gcggtgggtg gcttggtcaa gggagtagac
     5521 ttatcagctc aggaatgggt cgtgtccgca ctcatactcc ttgtaacctc tatcgtattt
     5581 ctagccattg gcttgctttt ggtgcaaatc cagtctgaac aggccatgtc ggttgtggca
     5641 aacttgctct actttgttct ggcaattatg ggaggctctt ggatgcctgt ttccctcttt
     5701 ccagattggg tgcagcggat ttgcaaactc ctgcccagct accatgtcaa tcagctggtg
     5761 accacctatg ctcaaaaggg agattttctc tggaaatcct tgctaattgt cctaggctat
     5821 gcgataattt tcttaggaat tgctctagtt ctgaatagaa aatcggagta acagtgaggt
     5881 aagctatgat ttttgaaaaa agaaaggtac cgctcatgtt cttcgtgggc atggtctttc
     5941 tctattttcc cttttactat gcccagtata gtcccaatcc gacagcagtt gtcctagcga
     6001 ctattctctt tgccttggtc tattgctccc tcttttatac cgaccacaag ctctattccc
     6061 tgctgggttg gttttacctg attggctata tcgccgtcat gagtttttgg ggcaatctgc
     6121 aattttccct ctttatctac aacctgtcca atatgctagg ctggtactac aaggaaactc
     6181 gaccgaccta tcggacggtg tcctatggga tcttgttact ggccattatc ctctgggctc
     6241 tctttggccc agttcctttt gagatgagag cctttgcact tgttattcac ttctttggct
     6301 tggccttgct gattttcagc tggattgaga ccaagcgtga agccctgcag caacgcctac
     6361 aggagcagaa tgcttccatc aatctcctgc tggctgagaa tgaacgcaac cgtatcggtc
     6421 aggacctgca cgatacactg ggccatgtct ttgccatgct gtctgtcaag gccgagctgg
     6481 tccagacttt gttagaccac gaccagattg accaggccaa gaaggaggtg gcagaccttc
     6541 agaccttggc caaggactcc atgcaggagg tccgccagat tgtccagtcc ctcaagcagc
     6601 acaccgtggc agaggaactc cagattttgc agcagatgtt gaacttggca ggagtggact
     6661 tagttgtctt gggtggggaa atagcagagc agctcagctt gcctgcccag aacaaactgg
     6721 ctatggtgct gagagagttg gctaataacc tgctcaagca cagtcaggct agcaaatgcc
     6781 gtttggtttt tgaaaaagac cagcaagaat tggttttgca ctacgaagac aacggtgtgg
     6841 gttttaccca gttgactggt caggaattgc ataccataaa ggacaggttg gtaacggtga
     6901 aaggcagttt ggagtttctt tctctagcca agcccacccg cctgtccatc cgaattccgt
     6961 ttgaggaggt ggtagaatga gaatcttagt cgcagaagat caagcactgc tgcgggacgc
     7021 cctctgtcaa ctcttgggtt ttcaagatgc ggttgaggct gttttacagg tagaaaacgg
     7081 cagtcaggct attgctcttt tggaaaaaga gtcagtagat gtggtgctct tagatataga
     7141 aatgcctgca aagacaggcc tagatgtctt ggaatgggcg cgggggcaag ccttgcctgt
     7201 caaggttgtc atcatgacca ccttcaagcg accgggctat ttcgagcggg cggtcaaggc
     7261 agatgtggat gcctacgtgc tcaaggaacg ctccatcgca gacctcatgc ggaccattga
     7321 aacggtcttg gcggggcgga aggaatactc gccagagttg atggacattc tcctaaccca
     7381 acgcagtccc ttgaccaact tggaaagtca actgctcaag ctggttgccg acggcttgtc
     7441 caataaggaa atcgccagcc agctccacct gtctgacggg acagtccgca actacatgac
     7501 ctctatcctg tccaagctgg gtgccgaaaa ccgcacggct gctgttaagg tggcacagga
     7561 aaagggttgg ttagtataaa cgaatgagat tggacatttg tccaatcttt ttatagacct
     7621 gatttttgac tgagttttta tactcttcga aaatcaaaat cagacgttgt tgacttgatt
     7681 tgatgagtga aaggctccag tggagtcttt cagcctgtta ccttgaaaca aaatagtaac
     7741 agggttctat ctgcgtaagt agtaacgaac tacgttcgcc ctatcttcaa cctccaaagg
     7801 ttccccaaac ctttggagct agtctttttt tgattttcat tgagtattat attgaaaaac
     7861 acctaaaatt ctgctacaat ttttttatga aaagacttta tgatgtgcaa caattactca
     7921 aacggtttgg tataattgtc tatatgggca accgtctcta cgatattgaa atgatgcaga
     7981 tagaattaaa tagaatctat caggcaggtg tcttagatcg gttggaatat atggaagcag
     8041 agcttgtttt gaggcgtgaa catcgtttgg aattagagta tcagaaatca agggagaatg
     8101 agtgatggaa acattttggg tatttttagt atttttggca gtattgggag cctggatggg
     8161 ttttaattac tggagattgc gtcgtgcagc gactgtgatt gataatgcag aatttgcaga
     8221 aaagatacac ggaggtcagt tgattgacct gcgagacccg agtgagttta atcgtaagca
     8281 tatcttaggt gcgagaaata ttccttacca gcaattgcgc cagtctacag ctgctttgcg
     8341 taaggacaga cctgtcttaa tctacgagaa tgatcgtggt cagttggtaa cgcctgcagc
     8401 tcttcatctg aagaaacagg gctttaacga aatctatatt ttaagctatg gtctaaacgg
     8461 atggaatggt aaggtaaaaa cgactaagta gcattgtcag taggctagag tattttctta
     8521 tgcaataaga cggataaggg caaaaatatg gaaaagaaat ttgaaaagtt gtcattggca
     8581 gaaattggca ttatttcttt tatgggactt attttattga ttagcttagg cgctagttgg
     8641 tataaaaatg ctcaaagcga aactagtgat acaaagacag tacagcaggt gcccaatcag
     8701 gaaaaaacgg ttgatacatt atttcaggat gctgagaatg ctgtcattgt attagaagga
     8761 acacatactg aagagaaact tgcagaggca caatctgcag taaacaaggt tacagatgcg
     8821 actaaaaaag caacattaca gaaacggatt gaccaagtaa aagagctagt gggacagcag
     8881 acagatgctc aaacacaggc taagctaaaa gcggagttgg tcagggtgga agcagcccaa
     8941 aaagtagctg atgagaaaaa agtatctgaa acagggacta gtcagtcaac ggaccttgct
     9001 gaagctagtg agaccttaca agaatcctat gtagtgccta atcaaaatat atatggaaat
     9061 caaagtgtga gttcaacaag ccagtataca agcagcgtgg cgtcaactag tacaagtact
     9121 tcagtccctc cgactagtga aggagcacct gtggtagaaa ctccaacagt ttctgaaaca
     9181 ccggcggaaa cgacggattc aacaactagc ctggttgagt cagtagaaac agttccaaca
     9241 gctgacgaga gttcaccaga taatccgtag actttttagc tactggttct ttatggcgta
     9301 ttagcatact ggaaaaaaat agctatttgt atttcaacac caattttttc ttgtaacgat
     9361 ctagtttaaa tacatacgat aagagtgagg aatttcgcgt aaaacagaat ttccttgctt
     9421 tttattttta ccattttgtt gaatagtgcg agaatgattt cccgctatag cggttgagag
     9481 tgtatttggt agaaggttga aaaagttggt gcaaaaagga aagtaggaag tgcacttggt
     9541 ttcagcaacg agcttcattt tttgctataa tataccttat gagaattgta tcgggaagct
     9601 atggtggaag accactgaaa acgttagatg gaaaaacaac cagaccgaca tcggataagg
     9661 tccgtggagc catgttcaat atgattggtc cctattttga gggtggtaga gttcttgact
     9721 tgtacgccgg tagtggtggt ttatctatcg aggctatatc tcgtggcatg gagtcagcag
     9781 ttctggttga gcgagatcga cgagcacaag ccattatccg tgaaaatatt gcaatgacta
     9841 aggaagcaga taaatttcag cttctagcaa tggaagctag acaggctttg aacagtgtgg
     9901 gaggtccttt cgacctggta ttcttggacc ctccttatgc agagcaggaa attgaagatg
     9961 tgataacaga actttgtcgg cgtgacttaa tagcagatga tgtcatggta gtctgcgaaa
    10021 cggataaggc ggtctctctt ccggaggaaa tagcagagct ggggatatgg aaagagaaac
    10081 tctacggaat tagtaaggta acagtatatg tcagataaga ttggattgtt tacgggctca
    10141 tttgacccca ttaccaatgg acacttggac ttaattgaac gagcgtgtgg actatttgat
    10201 aaattatatg tgggagtttt taccaatcct aaaaaggcag gactcttgac aggtttggag
    10261 agaaaagcca ttttggagaa gctttttgta ggaatggaga atatcgaggt cgttctgtct
    10321 gaaaatgaat tggtagtcga tgtagctaag cgctatggag taacacattt ggtccgagga
    10381 ttgcgtaatg cgaccgattt ggaatacgag tcaagttttg atttttacaa tcgtcagttg
    10441 gcgccaggtt tggaaaccat ctatttaatc gccaagccag agcttaagtt cgtatcatcc
    10501 agccaggttc gagaattgct gtatttcaaa caggatatag acccttatgt tccagaaatt
    10561 gttagtgagg agattagaaa gaatgaagaa aaataggaag ttaaccttaa ttgcatctat
    10621 tgttttagga actcttttga tttggtttgc cgtttttgtt cgtctgcctt attatctgga
    10681 aagtccaggt ggagctgccg atattcgtca ggtactaacg gtgaacaatc aaatagacca
    10741 agaggatggc tcttacaact tcacctatgt atcagtccaa caagccacag ctttacaact
    10801 attcgctgct cagtttgatc cctatacgac agttcgttcc tctgaagaga tgacaggagg
    10861 ggccgacaat gaggagtatt tccgtatagc acggttttac atggaaactt cgcaaaatat
    10921 ggcgaagtat caaggattga ccttggcagg aaaagaagtc aatcttgatt tctttggtgt
    10981 ctatgtgctt gccttgacgg atgattcaac ctttaaaaaa gttcttaata ttgccgacac
    11041 tgttgtgagt atcaatggaa aaacatttga gtcttctcct gatttgatta aatatgtgag
    11101 tggcttggag gttggaagtg atgtaacagt tggttatatt agcgctggtc aggaaaaatc
    11161 tgcggacggt aagattatca aattgtctaa tggcaaaaac ggaataggca tcactctcgt
    11221 agaccataca gaggttcaaa gttcggttcc aattgatttc caaacgggag atattggtgg
    11281 tccaagtgct ggtctgatgt ttacacttgc catctatact caactcgcag agcctgactt
    11341 acgtgatggt cgaattattg ctggtacggg gactatcgag caggatggaa aagtaggtga
    11401 cattggtggg gctgataaga aggtcatttc ggcagctaag tcaggtgcaa gtatcttttt
    11461 tgtaccaaac aatccagttg atgtagaagt tttgaagaaa aacccaaagg ctaaaacgaa
    11521 ctatgaagaa gctaaggaag cagccgagaa agcaggtcta gatattgagg tagttcctgt
    11581 gaaaactgtt caagaggcta ttgattattt gaagaaaaca aagggcgagt aaatcgtcct
    11641 tttttgctag agaataatat tataattgtc agaaatttct cctaaatggt aaagtagaag
    11701 aatttccaga taaatcgtgt tataatgata aggttaagtg tgagaattta gattctgacc
    11761 agatagatta ttaatcaaat gatttttacg tatgaagatt ttataagata ggaggacaaa
    11821 tgaaaaaaga aatttcacct gaaatgtata actataataa ataccctggt ccgagttttg
    11881 ctcgtgtcgg agataaggtt gtatctgaaa atattgaatt ggaccttttg gaagattaca
    11941 agaatgcttt tgaccagacg atttttggtc aacgtttttc acaactcatg ctcaaatttg
    12001 attatattgt gggagattgg ggcaatgagc agctgagatt aaggggattc tatacagacg
    12061 ataaaaatgt taaatcagac ttaaaaatca gccgcttgga cgactatttg acggagtatt
    12121 gtaattttgg ttgtgcttat ttcgtcttag ccaatccgaa tccacaagac ttgcctgctg
    12181 aggaggagga gagaccgcgt cggaagcgga gtcgttctcg caatcggaac agaaatcagc
    12241 agcggacgga gcctattgcg ccaaaagaaa acaaaagtaa tcgcaatgac cgtcagaaaa
    12301 aagggaagca gcaaaacaaa cagtcggaac agcgtcattt tactatgaag aatgctggtg
    12361 ttaaggcgag tcaaacggag cgttctcaaa aacgtgagcg taagcagagc aagcgtgaga
    12421 tgaatgaagt gaagcgtaac tttgttatcc gccagaaata gggagaaata tgaaaccatc
    12481 aatttatgca tttagtcaag ccaatctggt tgattggatt ttagaaaacg gagagaagaa
    12541 gttccgtgcc actcaaattt gggaatggct ttaccgttct cgtgtccaat cttttgcgga
    12601 aatgaccaat ttgccaaaat ctttgattga aaagttggaa gaacattttg tagttaatcc
    12661 gctcaaacaa cggattgtac aggagtctaa ggacggtact atcaaatacc tttttgaact
    12721 gccagatggc atgttgattg agacagttct catgcaccag cactatggcc tttcagtctg
    12781 tgtaacgaca caagttggct gtaatatcgg ctgtacattc tgtgcatctg gattgattcc
    12841 taaacaacgt gacttgacca gtggtgagat tgtggctcag attatgttgg tgcagaaata
    12901 cctagatgag cgtaatcaga atgagcgcgt cagccatatc gttgtgatgg gaattggtga
    12961 gccgcttgat aactacgata atgttatgac cttcttgcga gtggttaatg acgacaaggg
    13021 cttggcaatt ggtgcccgtc acatcactgt ttctacgtca ggtttagcac caaaaattcg
    13081 tgagtttgca cgtgaaggtg tccaagttaa cttggcagta tctctacatg cgccaaataa
    13141 tgaccttcgt tcgagcatta tgcgcattaa ccgcaaattc cctatagaag ttctttttga
    13201 agcaatcgaa gactatatca aagtgaccaa ccgccgtgtg acttttgagt atattatgct
    13261 caatgaggtc aacgatggag tggaacaggc gcaggaacta gctgatttga ccaagaacat
    13321 tcgtaaattg tcttatatca acttgattcc ttataaccca gttagtgaac atgaccagta
    13381 cagtcgttca accaaggaac gtactcttgc tttctttgat gttttgaaaa aaatggagtt
    13441 aactgtgtcg ttcgtcagga acatggtaca gatattgatg ctgcctgtgg tcaattgcgc
    13501 tccaatacgc tcaagaaaga ccgtgaaaaa gcccgggctc gtattgtagc cgcaaaagca
    13561 aaggcaggta ttcgagcatg agaaagttct tccaagcaga tggaaacttg accaatctag
    13621 gaagacagat ttgtaagacc ttagctggag cctatgctct ggctattgtt ttactttgtt
    13681 tcctaccaca gacttggtac ccacagtata aggatttctc gaccccagga attattcaac
    13741 ttggccgtct ctatctttta ccaactcctt tcaacagcat cgtcaacggg gacaaggtag
    13801 atagtttggc ggacctaggg tggattttct tgcaaaacat cactaatatt tttctacttt
    13861 tccctctgat ttttcttctt ttgtttctta gggaagaatg gcggagcctt cgagcggtga
    13921 ttcgttatag tttttgtatc agtcttttta ttgaatgcac acaattacta ctagatttgc
    13981 tgattgatgc ccaacgcgtc tttgaaatcg atgatttatg gaccaataca atggggggaa
    14041 tactagccta ttttacctat tcattgataa agagagggat aatcctttcc aaacaggagt
    14101 cttagaacct accgtcacag gttcagaatg tccatctaag gctattgttt atgctacaca
    14161 ttgttatttt tttgaaatac agtcagtatt cccttaaaaa tgaccttgac tagctaaagc
    14221 tatctttgat atgagcgctt cgctcgtgag tacagttttt tagaaagtca cttaagcaga
    14281 gtggcttttt gttagttttt aatggattca ggcaatggaa actgaatttt caaattgttt
    14341 agaaaatttc taaaaattac ttgaccattt cgattttgta acgtataatt acaagtaatc
    14401 agaaaaagta ataaacactt tgtctttaga gagtctatgg ttggtgtgaa tagatgatag
    14461 aggtttatga aatgggctga tgccaaacaa gcattcgtat ccattttatg gattgtctgg
    14521 cttagtaaag tgagtttggt tcgatgaatt cgaaacaatg agagttcggt ccgcacccct
    14581 tatcgtgcgc cttcttgtta gaggagaaaa agttatggac ttttctagtc gagtccatag
    14641 tgaggtggca ccgtgtccat tgacgcccac gcaggatgtt ttttcctgcg tgggcttttt
    14701 cattgtcttt gtagattgct tttatatttt gtcacgatca acttgcctaa cttgacttat
    14761 gtctggcctt gatctcttat caaaaagcaa tctatttcga tgatgataga tattatgagg
    14821 taagcaatat gaaacgatgg cgtagcaatt aaggataagt aaagaaggat taaactcttg
    14881 accttattgg tctcttgtag cgaatgttga aagagggaat tatatgaaag aaaaaggtta
    14941 ttttggacag tttggtggta gttttgtacc agagcaaatt caggttttgt tggatcagtt
    15001 ggaggagacg tttgaacagt accgcaacga tccagaattt ttggcggaat accaagctta
    15061 tctgaaagat tatgctggtc gtgaaacgcc attgtatgtt gcggaatcat tgagcaagga
    15121 gcttggtgga gcgaaaatct atctcaaacg ggaagatttg aatcacttgg gctcacataa
    15181 gttaaacaat gtgctggggc aaattctgtt ggcaaaaaga atggggaaaa ctcgagtgat
    15241 tgcagaaact ggtgctggtc aacatggggt tgcgactgct gctgttgcgg cacgatttgg
    15301 tttgggctgt gatgtttata tggggcagaa gatgtcaaac gtcaacggct aaatgttttc
    15361 cgaatggaga tgatggggcg cgtgttcatg ctgtaacgga tggaactcaa accttgaaag
    15421 aggcagttga tgcggcattc ggtgcatgga tggcagattt agatgccttc tatgttttgg
    15481 gatcagcagt cggtccacat ccctatccga caattgtcca tgagtttcaa aagattatta
    15541 gtctagaatc tcgccgtcag attttagaaa aagaaggtcg cttgccagac tacgttattg
    15601 cctgtgtggg tggtggttcc aatgccatcg gtgcattttc tcaatattta ccagatgaat
    15661 cagtcaagct tatcggtgta gaagctgctg gtaaaggagt tgatacagaa ctgcatgcgg
    15721 cgactatgac taagggaaca gctggtgtgg ttgatgggat gaggactatt cactctttga
    15781 tgaaaatggt ggagttgcac ctgtttattc catttcagca ggtttggatt atccaggcgt
    15841 tggaccagaa catgcccact ataaggaaac tggtcgtgtt aattatgtcg cagcaacaga
    15901 tgatgaggct gttaatgccc ttctgacctt aagtcgtaca gaaggaattc ttccagccat
    15961 tgaatcttct catgcgattg cagaagctat tcgattagca ccacaactag ataaagataa
    16021 aatcattatt atcaatgttt ccggtcgagg agacaaggat gtggcggcta ttgcggatta
    16081 tttggagaat agataaaaga aatcccctga cggttgctta tcgtcagggg atatttgctt
    16141 atgtgacttt tagtccgtca cgccccgtaa ggtgcgtgac aaataaacca cccgctatgc
    16201 gggtgcggcg acgaaggtta tacaaaaaaa ctccaaacgc gatacaataa aggtgttcaa
    16261 gcaaattgta aagcgaaagg agaaaaatat ggcgcaaaaa gcacatagtt tatcacatac
    16321 caagtggctg tgttctacca cattgtcttc acacctaagt atagacgaaa aatcatctat
    16381 aatcaatatc gaagtag
//
LOCUS       DATUCG010000049        15773 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.050, whole genome shotgun sequence.
ACCESSION   DATUCG010000049 DATUCG010000000
VERSION     DATUCG010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15773
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.050"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            <1..734
                     /locus_tag="VCA20_001206"
     CDS             <1..734
                     /locus_tag="VCA20_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HEP1835035.1"
                     /translation="GHTHTILREPSQVGGVSICQAGQYGRFVGHLSLRCFADGRHEVL
                     AYDLIEVEKLTQEDSAVKAIIDQMKSERDAAFSKPIAILPQALDGERDSIRQGLSTLA
                     PVICQALFEQASQLGLQANGAVINGFGIRASLSAGSIFYSDLVKVLPFSKRVLLVRIK
                     GSDLVASLKTGLHPQMWGIVPGGEVFVVNGRAVESDKDYQIVTNSFVWSGKDNYDDFH
                     KAEIVQDLGLDIDIISEFFKRQYGD"
     gene            736..1230
                     /locus_tag="VCA20_001207"
     CDS             736..1230
                     /locus_tag="VCA20_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEP1835036.1"
                     /translation="MELRRPTIADKETILEMLAEFEKAGSAMDGGFISKDVDFEEWLE
                     KLKLAELGLELPKGFVPYVQYVSFDPTGRALGFLNLRLRLNDFLLKKGGHIGYSIRPT
                     ERGKGYAKDQLRLGLQEAASKNISKVLVTCLTDNQASRRTIVVCGGVLEDVRDGVERY
                     WIED"
     gene            1417..1974
                     /gene="nrdG"
                     /locus_tag="VCA20_001208"
     CDS             1417..1974
                     /gene="nrdG"
                     /locus_tag="VCA20_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEP1835037.1"
                     /translation="MNNPKPQEWKSEELSQGRIMDYKAFNFVDGEGVRCSLYVSGCLF
                     HCEGCYNVATWSFKAGIPYTKELEDRILADLAQPYVQGLTLLGGEPFLNTGTVLPLVK
                     RIRKELPDKDIWSWTGYTWEEMMLETPDKLELLSYLDILVDGRYDRTKRNLMLQFRGS
                     SNQRIIDVQKSLQEKRVVLWDKLKK"
     gene            complement(2068..2140)
                     /locus_tag="VCA20_001209"
     tRNA            complement(2068..2140)
                     /locus_tag="VCA20_001209"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2105..2107),aa:Lys,seq:ctt)
     gene            complement(2149..2342)
                     /gene="ssrS"
                     /locus_tag="VCA20_001210"
     ncRNA           complement(2149..2342)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VCA20_001210"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(2414..3694)
                     /locus_tag="VCA20_001211"
     CDS             complement(2414..3694)
                     /locus_tag="VCA20_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEP1835038.1"
                     /translation="MPANLALRMRPKSIDEVIGQEHLVGPGKIIRRMIDANMLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATTDNQKRLQEIAEEAKFSGGLVLLLDEIHR
                     LNKTKQDFLLPLLENGNIIMIGATTENPFFSILPAIRSRVQIFELQPLQTSHIRQALE
                     LALTDSERGFDFPVTIEPEALDFLANATNGDLRAAYNSLELAVLSTKESEGGRHIDLD
                     AVENSLQKSYISMDKNGDAHYDILSALQKSIRGSDVNASLHYAARLIEAEDLPSLARR
                     LTVIAYEDIGLANPEAQIHTVTALEAAQKIGFPEARILIANVVIDLALSPKSNSAYLA
                     MDAALADLRKNGHLPIPNHLRDGHYAGSKELGNAIGYQYPHAYPEKWVDQQYLPDKLL
                     AADYFTANDTGKYERALGMTQEKINHLKQKKKKS"
     gene            3764..4099
                     /locus_tag="VCA20_001212"
     CDS             3764..4099
                     /locus_tag="VCA20_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HEP1835039.1"
                     /translation="MKELTVSVLEEYLRDHYGTTVPEQSLFMKLVEEIGEVAELLNQR
                     AGRKMMDSEDDSSARLAEELADVIHYAVALAAVNQLDLTKSILEKDERASVKYGREIN
                     LLEFIEKRK"
     gene            4101..4571
                     /locus_tag="VCA20_001213"
     CDS             4101..4571
                     /locus_tag="VCA20_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEP1835040.1"
                     /translation="MAKHGFLDVLEAALDKHFTYDYELNWDKKNHAVELAFILEAQNA
                     AGIETVDDEGNASAEDIFLEEYVLFYNQDKSRFDEEDYLVALPFDAKKGFSAEFLTYF
                     AGFLKDTADQGLDDLMDFLADPEAQEFAIAWDGEAFEKGRADLVEGEFYPYPRY"
     gene            4573..4926
                     /locus_tag="VCA20_001214"
     CDS             4573..4926
                     /locus_tag="VCA20_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEP1835041.1"
                     /translation="MVHEMLLAPKPFEMMKSGQKTIELRLYDEKRKHIQIGDRIRFYC
                     TENQTQTIEVQVLDLHIFDNFAQLYKELDLLSCGYTQSSIREAKPEDMEVYYSREQLE
                     QYGAVGIELRVIDSI"
     gene            5396..6349
                     /locus_tag="VCA20_001215"
     CDS             5396..6349
                     /locus_tag="VCA20_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEP1835042.1"
                     /translation="MNSWQELMIHVHRDAEEAVSNLMIETGSQGVAISDSADYVGQED
                     RFGELYPEVEQSDMIAITAYYPDTVDIEEVKADLATRLADLTGFGLETGQISLESQEL
                     AEEDWADNWKKYYEPARITHDLTIVPSWTDYEATAGEKIIRLDPGMAFGTGTHPTTKM
                     SLFALSQVLRGGETVIDVGTGSGVLSIASSLLGAKEIYAYDLDEVAVRVAQENIDLNA
                     NTSNIHVAAGDLLRGIDIEAEVIVANILADILIYLTEDAYRLVKDEGYLIMSGIIADK
                     WDMVRASAEAAGFFLETHMIQGEWNCCVFKKTTDRSGVIGG"
     gene            6351..7097
                     /locus_tag="VCA20_001216"
     CDS             6351..7097
                     /locus_tag="VCA20_001216"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEP1835043.1"
                     /translation="MQQYFINGRAPQGVFQISDKDTAKHMFSVMRLQADDQIVLVFDD
                     GIKRLARVVDSQNQSVEIIEELTDNVELPVSVTVAMGFPKGDKLEFVAQKATELGMAA
                     LWAFPADWSMVKWDGKKLAKKAEKLEKIAQGAAEQSKRNRIPTVHLFEKKADFLAQLA
                     GFDQIILAYEESAKEGEQANLVKILSGLEAGQSVLVIIGPEGGVSPEEVAAFEGVGAV
                     KTGLGPRILRAETAPLYALSTISYTTELLR"
     gene            7111..8373
                     /locus_tag="VCA20_001217"
                     /pseudo
     CDS             7111..8373
                     /locus_tag="VCA20_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939093.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8384..9100
                     /locus_tag="VCA20_001218"
     CDS             8384..9100
                     /locus_tag="VCA20_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HEP1835044.1"
                     /translation="MFALGTIINTVTIALAGLLGSWFGHLLKERHQSGLTVASGLAVL
                     FLGISGSLEGLLTVVDGQLKSQNSMLLVLSLALGILIGEGLHIEGWFERLGIWLREKS
                     GNSQDGQFLDAFLTASLTVCIGAMAILGAIQDGLTGDYRLLAVKSILDFVIILIMTSS
                     LGKGAGFSAVPVFLFQGSITLLARLIEPLMTVQALANLSFIGSALIFCVGINIIWDKK
                     IRVANMLPAIVIAVIWSYFT"
     gene            9341..9898
                     /locus_tag="VCA20_001219"
     CDS             9341..9898
                     /locus_tag="VCA20_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835045.1"
                     /translation="MKRLSTPIFAAFGFFLIVFVALNQFGRVPGTSYQIISELVLYNQ
                     ATEQTYEFENHEVRKAFIRQLSSTSSRGFQASDSRTSLETTYHETIMSENLISSIYGG
                     EEGRIMEFRHPRKTALGNLVFASESVSYPVFARKYGNIRLETTILCQEFRVEQRLANQ
                     SNWVDLGTYTVKSPKETRYVPIYWE"
     gene            complement(10020..12461)
                     /gene="sntA"
                     /locus_tag="VCA20_001220"
     CDS             complement(10020..12461)
                     /gene="sntA"
                     /locus_tag="VCA20_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein SntA"
                     /protein_id="HEP1835046.1"
                     /translation="MNFRFSKCAVALTLALLAASNPKLAQAEEILNTTPASSTEASQA
                     VPVESDTTEEADNTESPAPATTEAENPSSSETAETSDPTSETTDTTTSEARTVTPVAT
                     ETSQPVEGQTVDVRILATTDLHTNLVNYDYYQDKPVETLGLAKTAVLIEEAKKENPNV
                     VLVDNGDTIQGTPLGNYKSIVDPIEEGEQHPMYAALETLGFDVGTLGNHEFNYGLAYL
                     EKVIRTANMPLVNANVLDPTTKDFLYTPYTIVKKIFTDTEGKKVTLNVGVTGIVPPQI
                     LNWDKAYLEGKVIVHDAVEAVRDIIPTMRENGADIVLVLSHSGIGDDQYEIGEENVGY
                     QIASLSGVDAVITGHSHAEFPGTAEKPSFYAKYSGVDDTNGKINGTPVTMAGKYGDHL
                     GVIDLNLVFKDGKWTSTSSKAAIRKIDTKSSVADGRIIDLAKEAHNETIKYVRQQVGE
                     TTAPINSFFALVQDDPSVQIVNNAQIWYAKQQLAGTSEANLPLLSAAAPFKAGTRGDA
                     SAYTDIPAGPIAIKNVADLYLYDNVVAILKVNGAQLKEWLEMSAGQFNQVNPSSTEPQ
                     NLVNTDFRTYNFDIIDGVTYQYDITQPNKYDRDGKVVNETASRVRNLQYNGRDVTADQ
                     EFIVVTNNYRANGTFPGVREASVKYLLNLENRQAIINYIIAEKVINPTADNNWTFTDS
                     IKGLDLRFLTADRAKSLVADQEGIVYLQASTASEGFGEFKFVYTEPKVVTPDEQQSGQ
                     GNTGQDIVLESGQRITLPVVNPPAPAPQHKLASPHSQASTKTLPATGEATSMLSLLGL
                     TLIGFAGAWTKKKEH"
     gene            12717..14918
                     /locus_tag="VCA20_001221"
     CDS             12717..14918
                     /locus_tag="VCA20_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEP1835047.1"
                     /translation="MKEINYTGEEVVALTATYLPEEDVIFVKKALDFATEAHKSQFRK
                     SGEPYIVHPIQVAGILAGLKLDAVTVACGFLHDVVEDTDVTLDDMEAEFGPEVRHIVG
                     GVTKLGKVEYKSHEEQLAENHRNMLIAMSQDMRVILVKLADRLHNMRTLKHLRKDKQE
                     RISRETMEIYAPLAHRLGISTIKWELEDMSFRYLNEIEFYKITRMMSEKRREREELVN
                     EVVDKLREYTEERHLHGQIYGRPKHIYSIYRKMHDKKKRFDQLYDLIAIRCIMDTPSD
                     VYAMLGYIHELWKPMPGRFKDYIANQKANGYQSIHTTVYGPRGPIEFQIRTMEMHQVA
                     EYGVAAHWAYKRGGKATASEKELRWINNLIELQEGAGDAQTFVNSVKDDIFTERIYVF
                     TPDGAVRELPKDSVPIDFAYEIHTKVGERATGAKVNGRMVPLTTKLKTGDQVEIITSA
                     NSFGPSRDWVNLVKTHKARNKIKQFFKNQDKELSVSKGREMLQNLLQENGYVPNQYLD
                     RRHMDEVLQKTSYKTDESLFAAVGFGEVSAISIFNRLTEKERREAERAKAKAVADELV
                     NGGEVKHDNKDSLKIRHEGGVVIEGASGLLIRIAKCCNPVPGDEIVGYITKGRGVAVH
                     RVDCMNLKSQENYDVRLIDVDWEDENSTKEYMANIDIYGLNRSGLLNDVLKVLTNASK
                     NISSVNAQPTKDMKFATIHVSFGISNLATLTSLVDKIKSVPEVYSVKRTNG"
     gene            14920..15363
                     /locus_tag="VCA20_001222"
     CDS             14920..15363
                     /locus_tag="VCA20_001222"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEP1835048.1"
                     /translation="MKIVLQRVSQASVTIEGSIHGQIEQGLLLLVGVGPDDTQEDLDY
                     AVRKIVNMRIFSDEAGKMNKSVQDVAGKILSISQFTLFADTKKGNRPAFTGAAAPTLA
                     SQLYDAFNQALSAFVPVEVGVFGADMAVSLVNDGPVTIVLDTKNK"
     gene            15441..15626
                     /locus_tag="VCA20_001223"
     CDS             15441..15626
                     /locus_tag="VCA20_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835049.1"
                     /translation="MKKLLTNHFFYLTIAFLLILVIYFSGIDKRWIILASFLYFIPSQ
                     ILYRRRLRERLQEDQPE"
BASE COUNT         4370 a         3075 c         3930 g         4398 t
ORIGIN      
        1 gtggacacac ccacacgatt ttgagagaac caagtcaggt ggggggcgtc agcatttgcc
       61 aagctggtca gtatggtcgc tttgtcggtc atctatccct gcggtgtttt gcagatggac
      121 ggcacgaggt cctggcttat gacttgattg aggtggagaa actgacccaa gaagacagtg
      181 cggtcaaggc tattatcgac cagatgaaat cggaacgtga cgcagctttt tccaaaccta
      241 tagcgatttt acctcaggca ctagacggag agcgagacag cattcgccaa gggttatcaa
      301 ctctggcccc tgttatttgt caggctttat ttgaacaggc gagtcaacta ggtttgcagg
      361 cgaatggagc ggtcatcaac ggcttcggta ttcgagcttc cttgtcggcg ggatcaatct
      421 tttattcgga cttggtcaag gtcttgccct tttccaagcg agtactctta gttcgtatca
      481 agggcagcga tttggtggct agcctcaaga caggtctcca ccctcagatg tggggcattg
      541 tcccgggcgg tgaggttttt gtggtcaatg gcagagcagt cgagtcagat aaggactatc
      601 agattgtgac caattctttt gtttggagcg gtaaggacaa ctatgacgat ttccacaagg
      661 cagaaattgt gcaggacttg ggactggata ttgacatcat cagcgaattt ttcaaaagac
      721 aatatggaga ctagcatgga attaagaaga ccaactatag cggataagga aacgattttg
      781 gagatgctgg cagaatttga aaaagcaggc tcagctatgg acggaggctt tatctccaag
      841 gatgtggact ttgaggaatg gctagagaag ctaaaactgg ctgagctagg tttggaattg
      901 ccaaaaggat ttgttccgta tgttcagtat gtatcatttg acccgacagg tcgagccctt
      961 ggctttctca atctgcgatt acggctcaat gatttcttgc tgaagaaggg cgggcatatc
     1021 ggttattcca ttcgcccgac ggagcgtggc aagggctatg ccaaggacca gttgcgacta
     1081 ggtttgcagg aagcggctag taaaaatatt tccaaagtcc tcgtgacttg cttgacagac
     1141 aatcaagcca gccgtcgcac gattgtggtt tgtggtggcg ttttggaaga tgttcgagac
     1201 ggagtagaaa gatactggat tgaggattaa tgggagtgac aaactactga atcaaaacag
     1261 gaaaggaatt gagattggtt ccgcactaag cttgctggga tcaatccgag ctaaagcatt
     1321 ggaagtacca tttatggata aaaacagtct gtgtactggt aaaacggaat tcatccaaac
     1381 gatcagattt catttgctta tgctcaggta tttgaaatga ataatcctaa accacaagaa
     1441 tggaagagcg aggaacttag ccaagggcga ataatggact acaaagcctt taactttgtg
     1501 gatggagaag gagtccgttg ctctctatat gttagtggct gtctgtttca ctgcgagggc
     1561 tgctacaatg ttgcaacctg gtcttttaaa gctgggattc cctataccaa ggagcttgaa
     1621 gaccgcattt tagcagactt ggcccagccc tatgtacagg gcctaaccct cttgggtggc
     1681 gagccttttc tgaacacagg cacggtcctc ccacttgtaa agcgaattcg gaaagaattg
     1741 ccagacaagg acatttggtc ttggacaggc tacacatggg aagaaatgat gttagaaact
     1801 ccggataagt tagagttact atcctattta gatattctag ttgacggccg ttatgaccgt
     1861 accaagcgca atcttatgct gcagttccga ggctcttcca accaacgcat catcgatgta
     1921 cagaaatcat tgcaggaaaa gcgagttgtc ctgtgggata agttaaaaaa atagcccttc
     1981 acaatgtgaa agggttcttt ttgaccaaat tctctctaaa aacgagaaaa agccttgata
     2041 aatcaagact ttcctcatat caaccaatcc tccctgtagg gatcgaacct acgacccacg
     2101 gattaagagt ccgctgctct gccagctgag ctaaggaagg taaaaaataa aaatgctgta
     2161 ttggtaccgg ttattcatga attgtattga tcccgcacgc tagaagcagg tgggtgacgt
     2221 gtttctgact tagttgcttc tgggtgtacc agcctgcata ctgccaaaag atctttgtct
     2281 ccctagtaat acaaaaatag tcggtcaaca cataggtatg aattcgtata ccacagcagt
     2341 tcttatttat gatttaatat taccacattt tttaaaaaaa tcaagagaaa atcggcaaaa
     2401 aatgaaaacg ttatcaactt tttttctttt tttgttttag atggttgatt ttttcttggg
     2461 tcatgcccaa ggcacgctcg tatttgccgg tatcgttggc ggtgaagtag tccgcagcca
     2521 gtaacttgtc gggcaggtat tgctggtcca cccatttttc aggataggca tgcggatact
     2581 ggtagccaat ggcatttccc agctccttac tgccggcata gtggccgtcc cgcaggtgat
     2641 ttgggatagg tagatgacca tttttccgca aatcagccag agctgcatcc atagccagat
     2701 aggcggagtt agacttggga gaaagggcca aatcaatcac cacattggca atcaaaatgc
     2761 gtgcttctgg aaagccgatt ttctgggcgg cttcaagggc cgtcaccgta tgaatctgag
     2821 cctctggatt ggccaagccg atgtcttcgt aggcaatgac cgtcaagcga cgggccaggc
     2881 taggtaggtc ttcggcctca atcaaacgag cggcgtagtg gaggctggca ttgacatcgc
     2941 taccccgaat ggatttttgt agggctgaga gaatgtcgta gtgggcatcg ccgttcttgt
     3001 ccatgctgat gtaggatttt tgcaggctat tttccacagc gtccaagtcg atatggcgtc
     3061 cgccctcgct ttctttggta gacagtacag ccagttctag tgaattatag gcggcacgaa
     3121 ggtcaccgtt tgtcgcattt gccagaaaat ccagggcctc aggttcaatg gtgacgggaa
     3181 aatcaaaacc acgttcgctg tctgtcaaag ccaactccaa ggcctgtcgg atatggctgg
     3241 tttgcaaagg ttgcaactca aaaatctgca cccgactgcg aatggcgggt aaaatagaga
     3301 aaaatggatt ttccgtcgtc gctccaatca tgatgatatt gccattttcc aaaagaggga
     3361 gtaggaagtc ctgcttggtc ttgttgaggc ggtggatttc atcgagcagg agaaccagac
     3421 cgccagaaaa cttagcctct tcagcgattt cctgcaggcg tttttggttg tcggtcgtgg
     3481 cattaaaggt ccgaaaggca tacttggtcg tgccagcaat tgcggacgca atcgaggtct
     3541 tgccaatccc tggcggaccg tagagaatca tagacgacag catattggca tcaatcatgc
     3601 gacggataat ctttccagga ccgaccaggt gctcctgacc gatcacttca tcaatggatt
     3661 tgggccgcat acgaagggcg agattggctg gcataggctt cctttcagtg catttatggt
     3721 ataatcatta tactacaatt ttaggatttg gagagaaacg aagatgaagg agttgacggt
     3781 gagtgttttg gaagaatact tgcgtgacca ctatgggacg acagttccag agcaaagtct
     3841 ctttatgaaa ttagtggaag aaattggtga agtggcagag ctactgaatc aacgtgcagg
     3901 ccgcaagatg atggatagcg aagacgatag ctctgctcgt ttggcagaag aattagctga
     3961 cgttattcac tatgctgtag ccttggcagc ggtgaatcaa ctagatttaa ccaagtccat
     4021 tttggagaaa gacgagcggg cttccgtcaa atacggtcga gaaataaatc tattggaatt
     4081 tatagaaaag aggaaataga atggcgaaac atggcttttt agatgtgctg gaagcggcac
     4141 tggataaaca ctttacttac gattatgaac tcaactggga caagaaaaac cacgcagttg
     4201 agctagcttt catcttggaa gcacagaacg cggctgggat tgaaacggtg gacgacgaag
     4261 gcaatgcttc tgcggaggat attttcctgg aggagtatgt ccttttctac aaccaggaca
     4321 agtcccgctt tgacgaggaa gattacctgg tcgctctgcc atttgacgcc aagaagggct
     4381 tttcagcaga gtttctgacc tatttcgcag gttttctcaa ggacaccgcc gatcaaggtt
     4441 tagacgacct catggacttt ttggcggacc cagaagccca agaatttgcg attgcttggg
     4501 acggagaggc ttttgaaaaa ggcagagcag acttggtgga aggggaattt tacccatatc
     4561 cgaggtattg agatggttca tgaaatgtta ctggctccaa aaccttttga aatgatgaag
     4621 tctggtcaga agaccattga gctacgactt tatgacgaga agcgcaagca tatacaaatt
     4681 ggagatcgca tccgttttta ttgtacagag aatcagacgc aaacgatcga ggtgcaagta
     4741 ttagatcttc atatatttga taattttgct cagttataca aagaactgga tttattgtct
     4801 tgtgggtata cgcaaagcag tatcagagag gcgaaaccag aagatatgga agtttactat
     4861 tcacgagaac aattagaaca gtacggcgca gttggtatag aattgagggt aatagattct
     4921 atttgacatt ctgttttctc tcttgcttga ctgcaggcga aacttttgct attataaaaa
     4981 tagaaaaggc gttgagttcc agctcaacgc cctgacagga ccataacgat ggttcagctt
     5041 ttggacaaaa tagaccgcca gcttgaaatc tggggcggtc tattttttat ctagcatggt
     5101 tacgatgatt gtaatgatca aaccaagcat atttacaagt aagcttgcaa aagctatgat
     5161 gaccattagt tcatctgctg acaaatcagg ttctcctttc cgttggattt tgtgtctatt
     5221 tgcataggca ccaccacctt tcagaaatca gaaccaccgc tcagattttc ctgtcatttc
     5281 gtttattcga aaattatttt gattttactt accttataac attaacttgt aaaatgggct
     5341 agaaatcggt acaataatac agtgattgac gatagaaaag aaaaggaaaa caaacatgaa
     5401 ctcatggcaa gaattaatga ttcacgtgca tcgtgatgca gaagaagcag tttcaaatct
     5461 catgattgaa acgggtagtc aaggagtggc tattagcgac tcggctgact atgtggggca
     5521 ggaagaccgt ttcggcgagc tctatcccga ggtagagcag tcagatatga ttgcgattac
     5581 ggcctattat cctgatactg tggatattga ggaagttaag gcagacttag caactcgcct
     5641 ggcggatttg accggctttg gcttggaaac aggtcagatt agcttagaaa gtcaggaatt
     5701 ggcagaggaa gactgggcgg acaactggaa gaaatactat gagccagccc gcattaccca
     5761 cgatttgacc attgtgccgt cttggacgga ctacgaggcg acagcaggtg agaaaattat
     5821 ccgcttggat ccaggtatgg ctttcgggac gggaactcac ccgacgacca agatgagcct
     5881 ttttgctctt tctcaggtcc tgcgtggtgg cgaaacggtt atcgacgtcg gcacaggttc
     5941 aggcgttctc tccattgcta gctccctcct aggtgccaaa gagatttatg cctatgactt
     6001 ggatgaggtg gcggtgcgtg tagctcagga aaatattgac cttaacgcca atactagcaa
     6061 tattcatgtc gcagcgggcg atttgcttcg tggcattgac attgaggcgg aagtcattgt
     6121 tgccaacatc ttggcggaca ttctcatcta tctgaccgag gatgcctacc gtctggtcaa
     6181 ggacgagggt tacctgatta tgagcggtat cattgcggac aagtgggaca tggtgcgagc
     6241 atctgcggag gcggctggct tcttccttga gacccatatg attcagggcg agtggaattg
     6301 ctgtgtcttt aagaagacga ctgaccgctc aggtgtgatt ggaggctagg atgcagcagt
     6361 attttatcaa tggcagagca ccgcagggcg tgttccagat tagcgataag gacactgcca
     6421 agcacatgtt ttctgtcatg cgcttgcagg cagatgatca gattgtcctg gtctttgacg
     6481 atggtatcaa acgcttggcg cgcgtggtag atagccagaa ccaatctgtt gaaatcatcg
     6541 aagaactgac agataatgtc gaattacccg tttccgttac tgttgccatg ggctttccta
     6601 agggagataa gctcgaattt gtcgcccaaa aggcaacgga attaggcatg gcagccctct
     6661 gggcctttcc agctgactgg tccatggtca aatgggacgg taaaaaatta gccaaaaaag
     6721 cagaaaagct agaaaaaatc gcccaaggcg cagccgagca gagcaaacgc aatcgaattc
     6781 cgactgttca cttgtttgag aaaaaagcgg atttcctagc tcaactggca ggctttgacc
     6841 agattatcct agcctacgaa gaatccgcca aggagggtga gcaagccaat ttagtgaaaa
     6901 tcttatctgg cttagaagct ggacaatcag ttctggttat catcggtcca gaaggtggcg
     6961 tgtcgcctga ggaggtagct gctttcgaag gagtaggagc ggtcaagaca ggtctaggac
     7021 ctcgcatcct ccgagccgaa acggctccac tctatgcact ttctactatt agctacacga
     7081 cggaattgtt gaggtaagga gaatcgggag atgagtgaat tagaacaaaa ggaacaccga
     7141 gaattgattg tttcggatga ttgctttgct ctcattcaga aaatgagtga gttggattca
     7201 tcagcatatc agagatatga gattacaaat ccgaatattt tggctcatgt tgaaaagata
     7261 gttcaaccag cacttaaaca taatcttcag aaagccggat agcctaatat tcctaaggga
     7321 ttgttcaaag tggatttgcc ggttggagat ttgaataaaa tcaaagggga aacagctaag
     7381 tatcgtgcct ttgctaaagg tgcggatgga agaatcaatg cccatgcaat cttgacccct
     7441 gcacaagtgg aaattgcaaa gggagcgcaa gctgcagctg ctgttgcggg agtgatgcaa
     7501 gttgctggca tggttgttgg tcagtattac atgactgaaa tcaatggaaa acttaattcc
     7561 atgaaagaaa ctttgaatga tattaaggat tttcaagaaa gagaattcaa agggaccgtt
     7621 cgagctttga ttgttaacat ttctcagcta tcaacatttt cagcagatta tgttggaaat
     7681 actgaattat caagactaaa gttggtggag tgtgctaact tccgtgctga actaaccaag
     7741 ttactagaac aggtcaatct agctgttgaa gatattgtca ctaagggaaa aggattatct
     7801 tttgaaaagt atgaaaaact aattgaaaag ttaagtgcca atctgtttta tcaacaaacc
     7861 ttgctgagat tattagagga acttagtaag ttggacttag tattctcaca gggcgtgaca
     7921 tcagaggata gtagttatta tgtgtatgag tattatctaa acacctgtaa cagtttgggt
     7981 gaaaagatag ctgattggca taaaaacaag ataaaatcat ttcgcattga tttgaagcat
     8041 aataggcgtg tacaggaagg tgctatgaaa gtagtagcag atgttgccaa agagttggat
     8101 aagaatgccc atgttgctgc tggagtaggc tttcttggag gtatcgtagg tgctactgca
     8161 ggctttggaa ttggtaaagc tgctggagag ctaaataaag ccattcggta tattggtgtt
     8221 cgaaaacaga cgattaagca gatttcagaa caagaaagag tttcctatca atctagtaag
     8281 caactatcta ttaacagcta tgatgagaat gtagaattgc tgcttgaaga tggaaaggtt
     8341 tattatctat tgtcagatga tagtgaatta tagaggtaga ttcatgttcg cattaggaac
     8401 cattatcaat acggtgacca ttgccctagc aggtttgctg ggatcttggt ttggacattt
     8461 actgaaagaa cggcatcagt caggactaac ggtggcgagc gggctagctg ttttatttct
     8521 aggaatttca ggtagtttag aaggactgct gacagtcgtt gatggtcaac ttaaaagtca
     8581 aaatagcatg cttttggttc ttagtctagc cttggggatc ttgattgggg aagggctgca
     8641 tatcgaaggt tggtttgagc gcttgggtat ctggctccga gaaaagtcag gaaacagcca
     8701 agatggtcaa ttcttagatg cttttctaac cgcttccctg accgtctgta tcggtgccat
     8761 ggctatacta ggggctatcc aagatggact gacaggagat tatcgcttgt tggcggttaa
     8821 gagtattttg gactttgtta tcattttgat tatgacctcg agcttgggca agggggctgg
     8881 attttcagca gtaccagtct ttctctttca agggtctatt accctcttgg cacgtttgat
     8941 tgagccactg atgacggttc aggcacttgc caatctctct tttatcggct ccgccctcat
     9001 cttctgtgtc ggtatcaata tcatttggga caaaaagatt cgcgttgcca acatgttgcc
     9061 ggccatcgtg attgcagtta tttggtcata ttttacatga tctggtttac atttgcatac
     9121 ctgattggtt gttttttcaa aatcatattg atatactatt attgtgataa agtgaatggt
     9181 ttatcatcta aatagagaaa atagtctcga acacacgtta gcaaatagtg aatgtatcac
     9241 tccgaacgat tcgaatacac aggatcgtga tggattagtt catagctcaa tggtcgttgc
     9301 aattagcgaa gattgaggag atatgtgatg gagattgccc atgaaaagat tgagcacccc
     9361 tatttttgct gcttttggat ttttcttgat agtctttgtt gctcttaatc aatttggtag
     9421 agttccaggg actagctatc agattatctc ggaattggtt ttgtataatc aagcaacaga
     9481 gcagacctat gagtttgaaa atcatgaagt gagaaaggct tttattcgtc aactatcgag
     9541 cacgtctagt cgaggttttc aagcgtctga tagtcgtact tctctcgaaa caacctatca
     9601 tgaaaccatt atgagcgaaa atctgatttc ttcgatttat ggtggagaag aaggcagaat
     9661 tatggagttt aggcatccta gaaaaacagc tttagggaat ctcgtttttg cttcagaaag
     9721 cgtttcttac cctgtttttg caaggaagta tggaaacatt cgcttagaaa caacgatact
     9781 atgtcaggag tttagagtag aacaacggtt ggcgaaccaa tcaaactggg tagatttggg
     9841 aacctataca gtaaaaagtc ccaaagaaac ccgctatgta ccgatttatt gggagtagcg
     9901 atataaatat aaaaacggct caagtacgtc ttcgagctat ttttatatgc tttgattaat
     9961 ttatctagag agtgtatgag ggtaaaaaaa gagcttgaaa aaacaagctc ttgattattt
    10021 taatgttctt ttttcttagt ccatgcaccg gcaaaaccaa tcaaagtgag accgagcaga
    10081 ctaagcatag aagtagcttc tcctgttgca gggagtgtct tggtgctcgc ttgtgaatgt
    10141 ggactagcca atttatgttg tggagcagga gctggtggat ttaccactgg aagagtaatt
    10201 cgttgaccag attcgagtac aatatcttga cctgtgttac cttgacctga ctgctgttca
    10261 tctggagtca ctactttagg ttcagtgtag acgaatttga actcaccgaa cccttcgctg
    10321 gcagttgaag cctgtaggta gacaatacct tcttggtcag caactagaga tttcgcacga
    10381 tctgctgtca agaagcggag gtcgagtccc ttgatgctat ctgtaaaggt ccagttattg
    10441 tctgctgtag ggttgataac tttctcagcg ataatataat tgatgatggc ttggcggttt
    10501 tccaggttga gcaagtattt gacagaagct tcgcggaccc cagggaaagt accgtttgca
    10561 cggtagttgt tggtcacaac gatgaattct tgatcagctg tcacatctcg tccattatat
    10621 tgcaagttcc gaactctgct ggcagtttcg ttgactacct taccatcacg atcgtattta
    10681 tttggttgcg taatatcgta ctgataagtt actccgtcaa taatgtcaaa attgtaagtg
    10741 cggaaatctg tgttgactag gttttgtggc tctgttgagc ttgggttaac ttggttgaat
    10801 tgccctgcgg acatttccaa ccattctttg agttgagcac cgttgacttt taagatcgct
    10861 acaacgttgt cgtagaggta aaggtctgct acgttcttga tggcgattgg gccagcagga
    10921 atgtctgtat aggctgaagc atcgcctcga gtaccagcct taaatggtgc agccgcagat
    10981 aggagtggta gattagcttc tgaagtaccc gccaactgtt gttttgcata ccaaatctgt
    11041 gcattgttaa caatctgtac agaaggatca tcttgaacaa gggcaaagaa gctgttgatt
    11101 ggagcagttg tttcaccgac ttgttgacga acatatttga tggtttcgtt atgggcttct
    11161 ttagcaaggt cgatgatgcg accatctgct acagatgatt ttgtatcaat tttacgaata
    11221 gccgctttgc tagaagtaga tgtccatttg ccatctttaa agactagatt aaggtcaatc
    11281 acaccgaggt gatcaccgta ttttccagcc atggtgacag gagtgccgtt gattttacca
    11341 tttgtatcat ccacgccaga gtatttagca tagaagcttg gtttttcggc agttcctggg
    11401 aattctgcgt gggagtgacc tgtgataacc gcatcaactc ctgacaggct tgcgatttgg
    11461 taaccgacat tttcctcgcc gatttcatat tggtcatcgc cgatacctga gtgggaaagg
    11521 accagaacga tgtctgcgcc attttctcgc atggttggga taatatcgcg aacagcttca
    11581 actgcatcat gaacaatcac ttttccttct agataagctt tgtcccagtt gagaatttga
    11641 gggggaacaa tccctgtaac accgacattt agtgtaacct ttttaccctc ggtgtctgtg
    11701 aatatctttt tcacaatggt atatggggtg tagagaaagt ctttcgtagt cggatcgagg
    11761 acattggcat tgactagggg catgttggct gtacgaatta ctttttcgag gtaggcaagg
    11821 ccgtagttaa attcgtggtt gccaagtgtg ccgacatcaa atcccagagt ctctaaggcg
    11881 gcgtacatag gatgttgttc accttcttca ataggatcaa cgatagactt gtaatttcca
    11941 agaggagtac cttgaatggt gtctccatta tctaccaata cgacatttgg attttctttt
    12001 tttgcctctt cgataagaac agctgttttt gccagaccaa gcgtttccac tggtttatct
    12061 tggtaatagt catagttgac caagttcgta tggaggtcag ttgttgcgag aatgcgaaca
    12121 tcaacagttt gtccttcaac aggctgacta gtttcagttg caacaggagt gaccgtgcgt
    12181 gcttctgaag tagttgtgtc agttgtctcg cttgtaggat cactagtttc agctgtctcg
    12241 gaagagcttg gattttcagc ttcggtagtg gctggagcag gagattctgt attatcagct
    12301 tcctctgtgg tgtcagattc aactggtaca gcctgacttg cttctgttga agaagctgga
    12361 gttgtgttga gaatctcttc ggcttgagct aattttggat tgcttgcagc taagagagct
    12421 agtgtgagtg ctacggcaca cttactgaaa cgaaaattca taagaatcct ccattttctt
    12481 tttgtaataa tcttctacaa tcattataca gctaagaaat gcaaaattca agactaattc
    12541 tgaacgttat tttgaaaaac gagtgaaaat aacaagaaaa cgaaagaaaa caagaacgtt
    12601 cgctgaatct gaacttggtt tggattgttt gtaattagta gtcttatgtt agattccttt
    12661 ttcttttttc gtgaaaatgg tagaatagta ggaaatataa gaggaagagt agggatatga
    12721 aagaaattaa ctacactggg gaggaagtgg ttgctttgac agccacctat ttaccggaag
    12781 aagatgttat atttgtcaag aaagctttgg attttgcgac agaggcacac aagtcccaat
    12841 ttcgtaaatc gggcgaaccc tatattgtac acccgatcca ggtagcaggt attttagcag
    12901 gactcaagct ggatgctgtg acggttgcct gtggtttctt acatgatgtg gttgaagata
    12961 cggatgtaac cttggatgat atggaggctg aatttggtcc agaggttcgc catattgtcg
    13021 gcggtgtgac caaactgggt aaggttgagt ataagtcaca tgaggagcag ttggctgaaa
    13081 accaccgaaa tatgctgatt gctatgtctc aagatatgcg ggtcattctg gttaaactgg
    13141 cagaccgcct tcacaacatg cggactctta agcacttgcg caaggacaag caggaacgta
    13201 tttcacgtga aacaatggaa atctatgcac cgcttgccca ccgtctaggt atttccacta
    13261 tcaagtggga attggaagac atgtctttcc gctatctcaa tgagattgaa ttctacaaga
    13321 ttactcgaat gatgagtgag aagcgtcgtg aacgagaaga gttggtcaat gaagttgttg
    13381 ataagttaag agagtatacc gaggagcgtc atctacatgg tcagatttac ggtagaccca
    13441 agcatattta ctctatctat cgcaaaatgc acgataagaa aaagcggttt gatcaactct
    13501 atgatttgat tgccattcgt tgtatcatgg atactccaag cgatgtctat gctatgttag
    13561 ggtatattca tgaattgtgg aaacctatgc caggtcgctt caaggactac attgccaacc
    13621 aaaaagctaa cggctaccag tctattcata ccacggttta cggaccgaga ggtccaatcg
    13681 agttccaaat tcggaccatg gaaatgcacc aggtggcgga atacggggtg gcagctcact
    13741 gggcctacaa gcgtggtggc aaggcaactg ctagtgaaaa agagttgcgc tggattaaca
    13801 acctgattga actccaagaa ggggctgggg atgcgcaaac ctttgtaaat tctgtcaagg
    13861 acgatatttt tacagaacgt atttacgttt tcacgccaga tggtgctgtg cgtgagttgc
    13921 caaaggattc tgtaccgatt gacttcgcct atgagattca taccaaggtc ggcgagcggg
    13981 cgacaggtgc caaggtcaac gggcgtatgg tgcctctgac gaccaagctt aagacaggcg
    14041 atcaggtgga aatcatcacc tctgccaact ctttcggccc aagtcgtgac tgggtcaact
    14101 tggttaaaac tcacaaggct cgcaataaaa tcaagcagtt cttcaagaat caggacaagg
    14161 aattgtctgt ttcaaaaggg cgtgagatgc ttcagaacct actccaagaa aatggctacg
    14221 ttcccaacca atacctggac cgtcgccaca tggatgaggt cctgcaaaag accagctata
    14281 agaccgacga atcccttttt gcggcagttg gttttggaga agtttctgcc atatcaatct
    14341 ttaaccgtct gacagaaaaa gagcgtcgcg aagctgagcg ggctaaggcc aaagctgtcg
    14401 cggatgaatt ggtcaacggt ggcgaagtca aacacgacaa caaagatagt ctgaaaatcc
    14461 gtcacgaagg tggcgtggtc attgaagggg cgtcaggctt gctgatacgg attgccaaat
    14521 gttgtaatcc agtacctggc gatgaaattg tcggctacat caccaagggg cgtggcgtgg
    14581 ccgttcaccg tgtggactgt atgaacctca agagtcaaga aaactacgat gttcgcttga
    14641 ttgatgtgga ctgggaggat gagaattcga ccaaggaata catggcaaac attgacattt
    14701 acggtctcaa ccgctcagga ctgctcaacg atgtcctcaa ggttttgacc aatgccagca
    14761 agaacatttc ttcggtcaat gcccagccaa ccaaggatat gaagtttgcg accatccacg
    14821 tttcctttgg tatttccaac ctggcaacct tgaccagtct ggtggacaag attaagtcgg
    14881 taccagaagt ttactcagtg aaaaggacca acggataaga tgaaaattgt actacaacgt
    14941 gtatcacagg ccagtgtgac catcgaagga agtatccatg ggcaaattga acaagggctt
    15001 ttgctcctag tcggtgtggg accagatgac acacaggaag acctggacta tgctgttcgg
    15061 aaaattgtca atatgcggat tttttcagat gaggcaggca agatgaacaa gtctgttcaa
    15121 gatgtggcgg gcaagatttt atccatttcc caatttactc tttttgcgga caccaaaaaa
    15181 ggcaatcgtc cggcttttac aggagcggca gctcctaccc tagccagcca actctatgat
    15241 gcttttaacc aggccttgtc agcctttgtg cctgttgaag tgggcgtttt tggggcggat
    15301 atggcggtta gcctggtcaa tgatgggccg gtgactattg ttttggatac gaaaaataag
    15361 taaaactttt ggcaatggta tgtactctat ttgtgtattt tgatatacta atgttgaaaa
    15421 aaataggtat ggaggttgct atgaaaaaat tgctcacaaa tcatttcttt tatttgacga
    15481 ttgcctttct tttgattttg gtgatttact ttagtggaat agataaacgt tggattattt
    15541 tggcaagttt tctttatttc attccatcgc agattttata ccgtcgtcgc ctaagagagc
    15601 gactacaaga agaccagccc gagtaggcaa tgtcattaag ttaactaacc tatcacattt
    15661 tgtggtgggt tatttttatg ttacactaaa aataaaaggg gaaaagtcat gctagatcag
    15721 attaaagctc atttacttga tagtattaac gacatcgttt ctagtgccaa tca
//
LOCUS       DATUCG010000050        15614 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.051, whole genome shotgun sequence.
ACCESSION   DATUCG010000050 DATUCG010000000
VERSION     DATUCG010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15614
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.051"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            92..637
                     /locus_tag="VCA20_001224"
     CDS             92..637
                     /locus_tag="VCA20_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEP1835050.1"
                     /translation="MANVLFLVGSLRDGSFNHQMAKKAEAFLADKAQVSYIDLAKIPL
                     FNQDIETPILPEIAELRAQVDAADAIWIFSPVYNYAIPGIVKNALDWLSRSLDLSNPK
                     GPSILQDKITTVSAVANSGHDHLFKDFQHLLPFIRTQIAGEFTGSTINPEAWGTGVLE
                     LSDETLTKLEQQAANLLAAIQ"
     gene            complement(734..1846)
                     /gene="glf"
                     /locus_tag="VCA20_001225"
     CDS             complement(734..1846)
                     /gene="glf"
                     /locus_tag="VCA20_001225"
                     /EC_number="5.4.99.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HEP1835051.1"
                     /translation="MMSYDYLVVGAGLFGAVFAHEAAKKGKKIKVIEKRDHIAGNIYT
                     KEVEGIQVHEYGAHIFHTSEKEIWDYVNQFAEFNRYTNTPVANYKGEIYNLPFNMNTF
                     NKLWGMVTPAEAEAKIAEQRAVLGGKTPENLEEQAISLVGTDIYEKLIKSYTEKQWEK
                     PCTELPAFIIRRLPVRLTYDNNYFNDTYQGIPIGGYTQIVEKMLDHENIDVETNVDFF
                     ANKEDYLATYPKIVFTGMIDEFFDYQLGELEYRSLRFETEVLDMENYQGNAVVNYTDS
                     ETPFTRIIEHKHFEFGTQPKTIITREYSKTWKRGDEPYYPVNNDRNNHLYTAYKKLAE
                     QQENVIFGGRLGHYRYYDMHQVIGAALQCVRNEVGE"
     gene            complement(2071..3312)
                     /locus_tag="VCA20_001226"
     CDS             complement(2071..3312)
                     /locus_tag="VCA20_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEP1835052.1"
                     /translation="MKIAVAGTGYVGLSIAVLLAQHYQVTAVDVIKEKVDLINSKKSP
                     IQDDYIEKYLAEKDLNLVATLDAEKAYKDAEIVVIAAPTNYDSAKNYFDTSHVEAVIQ
                     MVLSVNPQALMVIKSTVPVGFTQSMREKFGTENIIFSPEFLRESKALYDNLYPSRIIV
                     SYESNSPQTVIEGAKLFAKLLQQGALKENVEVLHMGATEAEAVKLFANTYLALRVSYF
                     NELDTYAELKGLDTESIIRGVGLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYNDIP
                     QNMMTAIVESNRTRKDFIADQILKIAGYYDYSSHDQYSQLGEKEVIIGVYRLTMKSNS
                     DNFRQSSIQGVMKRLKAKGVKVIIFEPTLENGSTFFGSKVINNLNQFKSKSHAIVANR
                     YDSVLDDVLDKVYTRDIFRRD"
     gene            complement(3376..4482)
                     /gene="wecB"
                     /locus_tag="VCA20_001227"
     CDS             complement(3376..4482)
                     /gene="wecB"
                     /locus_tag="VCA20_001227"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEP1835053.1"
                     /translation="MKKVMLVFGTRPEAIKMCPIVNELKRRKGIETIVCVTGQHRQML
                     DQVLDVFNVVPDYDLSIMKERQTLFDVTSSILSKIEVILDEVEPDIVLVHGDTSTTFA
                     TALACFYKQIQVGHVEAGLRTYDTYSPYPEEFNRQSVGIIANYHFAPTQGSANNLIRE
                     GKNIENIYITGNTAIDALSTTVQSNFKHEVLDWAQDSRLIVITAHRRENLGEPMYQMF
                     RAIRRIIDEYTDVKAVYPIHMNPLVRQIADEVLSGCNRIRIIEPMEVIEFHNLLSNSY
                     LILTDSGGIQEEAPSLGKPVLVMRDTTERPEGIEAGTLKLVGTDEEVIYRNCKELLDN
                     TKYYSNMAQAVNPYGDGKTSCRIADILEKGYTEI"
     gene            complement(4494..5945)
                     /locus_tag="VCA20_001228"
     CDS             complement(4494..5945)
                     /locus_tag="VCA20_001228"
                     /inference="COORDINATES: protein
                     motif:HMM:NF024832.1,HMM:NF026018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HEP1835054.1"
                     /translation="MINKSIKLNASLNIIRSCLAIFFQLATLPYVSRVLGVENVGKIN
                     FGLSIVSYFSLLSLLGVNIYVEREGARFREDRRKFQRLGSEVFTLQMITTLFAYVVLL
                     LVLYFSPLLKEYRLLILLQSSTILFTTLGIEWVNIIYEDYFSITLRTIIVYIFSFILT
                     FTLVKSPNDYYLYSLIGVVSTIFIAVFNQIHCRKYFTPKLIFTRDIVRHFKSSLVFFV
                     NNLAVSIYVNIDVTMLGSMGGTYSVGMYSSAVKIYSILKSILAALYIVVIARLSFYAS
                     NNDFVNFKKLFTSIINWLILLLLPIASGLFIVAGEVVYILYGSAYAPASVTLKILSLS
                     LIFAIFGGVLTNCLNIPLGREKFNVYSTVTAAVVNAILNLIMIPLFHENGAAFTTVLA
                     EFSVFMIVLVSFKEWKKVIVAVEVKRNLLDAILGILLMFIVNHVVFSYFEFNILLTLM
                     VKIFLSVFSYFSILIFRKNKEMWKIFATIGLKK"
     gene            complement(5959..6873)
                     /locus_tag="VCA20_001229"
     CDS             complement(5959..6873)
                     /locus_tag="VCA20_001229"
                     /inference="COORDINATES: protein motif:HMM:NF012745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEP1835055.1"
                     /translation="MFFTVCIPTFNRSKTLIRTLESLRKQTFKDFEILVIDDGSEDDT
                     ESVVKNWSGEQSCRYFYKENGGKHSAINVGLDNAKGTFFLILDSDDWLIDEGLRILYN
                     YAKDIVDDNQYCGVLARAFDSNRREMIGELIPDEYLSMSYIDFHFGIGRKKSFLDCCE
                     CNKTVILQQYRFPIAENMKFVPEAWLFDQVGLNYSLVCTNEIVMMKEYLENGMTTDVY
                     FKDKHYLGFLQHYVSRLDIIIPEAKLSFPLQIVAWWRYWDCVRKDSAKNGVRVKKVTL
                     LGRIVYLLNPVIDITYKVMYRKLAKRGR"
     gene            complement(6875..7960)
                     /locus_tag="VCA20_001230"
     CDS             complement(6875..7960)
                     /locus_tag="VCA20_001230"
                     /inference="COORDINATES: protein motif:HMM:NF012744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEP1835056.1"
                     /translation="MRLNYVCQWDLNRKKTWSGTTWSLYKALEKKCNINEYDIRYTTL
                     EKLLMRLSKISFSYNGIGKANQFNAVIDRIDQGKLKALDTSFPSLQIGCIGKAENSYI
                     YEDLSASALEMIYLKYSKAFEKSNFSNASFQSIQEKVKRQNEVYRHSKAIFTMSHWVA
                     EEIKKSAGIDNVYCVGGGINLDKKDIDSSLKVGNKFLFVGRDFYRKGGDLVVKSFVKL
                     HKKYPYLELYIAGTQNIYGTEFKNIHWLGDLNKIQLVEYFNKCDIFCLPSEFEAYGLV
                     FGEALCFGLPCIGRNICEMPYFIEDGVNGYLIDGNDCDIFSELMERAIFNEEMKKIVR
                     DEKDKYLNKYSWDSVVDRLLSYIEEGE"
     gene            complement(7957..9153)
                     /locus_tag="VCA20_001231"
     CDS             complement(7957..9153)
                     /locus_tag="VCA20_001231"
                     /inference="COORDINATES: protein motif:HMM:NF016800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEP1835057.1"
                     /translation="MLELLLSLILQNTYQFNILGPVRLVDVLLFLLFSYCLIFCRGKV
                     TLIRDISFLIIIRIAYVMLTLGFFGNLSLGTAMSLGYFFEFILVWFIIMNFQTKVSFE
                     KIWNIGVILLVINNIYAIIELFFNGTARAGGFIGPAITCFALPYILYGLFKRGRKFDE
                     KKSQLISLIGIAGAIVSKQRTLIILILLNIFIYLLLSKRNLLKLLPKIIFSFIIIAFI
                     ILFAIPDSLKEYYIYKYNEIFWSLTANIDSSMHTRYVLWDASIGLFFSSPFFGIGSGM
                     FTRQSATQLQNFSSRLVTERVVGLSTHNFFLEYASELGIIGLFLTYFLIFRIFKDIKN
                     RAITYGVKNDFMFEVLFSSLLTLTLYDLFGQGTFLYFWNYTLVIMYSYLFYSRKKNHD
                     FVEKLI"
     gene            complement(9159..10055)
                     /locus_tag="VCA20_001232"
     CDS             complement(9159..10055)
                     /locus_tag="VCA20_001232"
                     /inference="COORDINATES: protein motif:HMM:NF012745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEP1835058.1"
                     /translation="MLGIVIVNYNSYQNTFNCVQSLFDYPVNYEHKIYIVDNGSSNNS
                     FDMLWNRYRDNHDVTLIKVNESKGYSAGLNRGIEQALKDGCGYFLLCNNDLIFPSGTI
                     DNMIIANQTEPKLGVVGTLIIGTDNGEQKSYKHRMTYYSHLFSKKPFAYFYRFPQNTI
                     SREKYYFDGMVSGCCFLLSKLTISKIGLLDENIFLFYEEDAIAHKLYKAELQALLLTD
                     QKIIHFGSITIGTQGTTYYLNRYKSSMYVLKKYCEINNFQLSVLFLLNYFSLLLKCIK
                     NNEYQKACKELLNYYIELCRGT"
     gene            complement(10065..11144)
                     /locus_tag="VCA20_001233"
     CDS             complement(10065..11144)
                     /locus_tag="VCA20_001233"
                     /inference="COORDINATES: protein motif:HMM:NF025072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEP1835059.1"
                     /translation="MSKKILVLAEDYPNKNGGVTLMYIHTRNTYYKQMGIEVTVLNFS
                     AKKSYDIDGISVITQKDYHKINKNYDILVIHAANIKHHYLFIRKFGSKFEKFLFFFHG
                     HEVMRITKSYPAPYPFISRDRFREFYQDRYDLIKLFIWKKYLPKIAYKSHFVFVSQWM
                     KDEFYKWTKLPYGLLDYSSSIIYNCIGEEFEKNIISDVSDFKYDFVTIRNNLDGSKYG
                     IDIVNQYAKNTPKARFLVIGKGEYFTHFEKSSNIEWRNHNLSHTEIVEILSYCKFAFM
                     PTKVDAQGLMMCEMAALGIPVITSNIPVCDEVFADFENVYLIDNDVKESSLDFVLNNS
                     RNIIKDSRFFAQNTIMKEVDLILNM"
     gene            complement(11336..12061)
                     /locus_tag="VCA20_001234"
     CDS             complement(11336..12061)
                     /locus_tag="VCA20_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEP1835060.1"
                     /translation="MPTYNCGRYISKTIESILAQTYQNWELIIVDDQSTDNTKDIVDG
                     YVESDSRIKYHLLPENSGAAVARTEAMKRSKGEFIAFCDSDDLWIPSKLTKQLEFMEK
                     NNVFFSCTSYEQIDETDFPLGKIVKSVKKVSYNRLLLDCPVGNSTVMYNVSKMGKFEV
                     PNIRKRNDDALWLKMLKVEPYIHGIPDVLMQYRIRKNSISSNKVDLIKYHWKLYRDIE
                     HLSIVRSVFHILFWGIIKIFRLK"
     gene            complement(12095..13312)
                     /locus_tag="VCA20_001235"
     CDS             complement(12095..13312)
                     /locus_tag="VCA20_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEP1835061.1"
                     /translation="MTKRPNIPFSPPDITQAEINEVIDTLKSGWITTGPKTKELERRL
                     SVFTGTNKNVCLNSATAGLELVLRILGVGPGDEVIVPAMTYTASCSVITHVGATPVMV
                     DIQKNSFEMEYDALEKAITPKTKVIIPVDLAGIPCDYDKIYTIVENKRSLYVASDNKW
                     QKLFGRVIILSDSAHSLGASYKGKPAGSLADFTSFSFHAVKNFTTAEGGSVTWRPHPD
                     LDDEEMYKEFQIYSLHGQTKDALAKTQLGSWEYDIVIPGYKCNMTDIMAGIGLVQLER
                     YPSLLNRRREIIEKYNAGFEGTSIKPLVHQTEDKQSSMHLYITHLEGFTLEQRNEVIQ
                     KMAEAGIACNVHYKPLPLLTAYKNLGFDMKDYPNAYQYFENEVTLPLHTNLSDEDVEY
                     LIESLKRIIFEVL"
     gene            complement(13322..14020)
                     /locus_tag="VCA20_001236"
     CDS             complement(13322..14020)
                     /locus_tag="VCA20_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEP1835062.1"
                     /translation="MHSGRTITKEYLKANEITGQYFDKIESRKFELFLKYATDRLLAL
                     ILLVLLSPVIIALSIWIKIDSKGSVFYRQERVTRYGRMFRIFKFRTMVTDADKVGSLV
                     TVGQDNRITKVGHIIRKYRLDEVPQLFNVLTGDMSFVGVRPEVQKYVDHYTDEMLATL
                     LLPAGITSPASIAYKDEDSVLEEYCSQGYSPDEAYVQKVLPEKMKYNLEYIKNFGIIS
                     DFKVMIDTVINVVK"
     gene            complement(14056..14772)
                     /locus_tag="VCA20_001237"
     CDS             complement(14056..14772)
                     /locus_tag="VCA20_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEP1835063.1"
                     /translation="MLGHRLLREKEDRMKGGLYHKVQIELAFNSNHIEGSQLTHDQTR
                     YIFETNTIGFENSSTISVDDIIETANHFTAFDFLLDSFQDDLNEIWIKDVHAILKRGT
                     SDSRKDWFQVGEYKKLPNEVGGQETTLPENVGLDMQELLRSYHSLSQTSFEDILDFHV
                     RFERIHPFQDGNGRVGRLIMFKECLKHGYVPFIIGDDMKYFFYRGLAEWGEQNGFLLD
                     TCLAAQDRFKHYIDYFRIED"
BASE COUNT         5283 a         3083 c         2128 g         5120 t
ORIGIN      
        1 tgcgtatgac gtggaatccg cagacctagg attgccaaca aaagacaaat acgatatact
       61 aaatttatgt ttttatgaaa aggaggccat catggcaaac gtattatttt tagttggttc
      121 actacgtgac ggttctttta accatcaaat ggcaaaaaaa gctgaagcgt tcttggctga
      181 taaggctcaa gtatcttaca ttgatcttgc aaaaattccc ctattcaacc aagacatcga
      241 gacacctatc cttcctgaaa ttgcagagct acgtgcacaa gtagacgctg cggatgctat
      301 ctggattttc tcaccagtat ataactatgc aatcccaggc atcgtaaaaa atgctctcga
      361 ttggctcagt cgttcgctcg atttatccaa tccaaaagga ccatctatcc tacaagataa
      421 aatcacaact gtctctgcag tcgcaaacag tggtcacgac cacctattca aagacttcca
      481 gcaccttctt ccatttatcc gcacccagat tgctggtgaa tttacaggtt caaccatcaa
      541 tccagaagca tggggaacag gtgtattaga gttatctgac gaaactctaa caaaactcga
      601 gcaacaagca gctaacctgt tggcagcaat tcaataatca aaaaaaccag actttctggt
      661 ttttttgatt aacattttga gagcattcca acttctgcca agaatcaatt actactttga
      721 tgacttctca aaatcattcc ccaacttcat ttctcacaca ctgtaaggca gctccgatga
      781 cttggtgcat atcgtaatat cgataatgac ccagacgtcc accgaatata acattctctt
      841 gttgttctgc caatttctta tatgctgtgt atagatggtt attgcgatca ttgttgactg
      901 gatagtatgg ctcatcgcct cgtttccagg tcttcgaata ctcacgagta ataatggttt
      961 tcggttgtgt accgaattcg aaatgtttgt gttcgataat acgagtaaac ggtgtttcgc
     1021 tatctgtgta gttgacaacc gcattccctt gatagttttc catatccaac acttctgtct
     1081 caaatcgaag gctacggtac tccaattcac caagttggta atcaaagaac tcgtcaatca
     1141 ttcctgtaaa gacaatcttt ggataggtag ctaagtagtc ttccttattg gcaaagaaat
     1201 caacatttgt ctcaacatcg atattttcat gatccaacat tttttcaaca atctgcgtgt
     1261 aaccgccgat tggaatacct tgataagtat cgttaaagta gttattatca taagtcaaac
     1321 gcacgggcaa gcgacggatg atgaaggctg gtaactccgt acacggtttt tcccattgtt
     1381 tctcggtgta ggacttgatc aatttttcat agatgtctgt accaactagt gagatggctt
     1441 gttcttccaa gttctcaggt gtttttcctc ccaatacagc ccgttgctct gcaatcttcg
     1501 cttcagcttc agcaggagtc accatacccc aaagcttgtt aaaggtattc atgttgaagg
     1561 gaagattgta aatctcaccc ttgtagttag cgactggtgt gtttgtatag cggttgaact
     1621 ccgcaaactg attcacataa tcccaaattt ctttttcgga agtatgaaag atatgtgcac
     1681 catactcatg aacttggata ccttctactt ctttagtgta gatgttacct gcaatgtggt
     1741 ctcgtttttc gataaccttt atttttttac cctttttagc tgcctcatga gcaaagaccg
     1801 caccgaacag ccctgcacca acaactagat aatcgtacga cattatttac ctctgaaaat
     1861 tacatttata catttcctaa caaggaacaa agtaagtctt tatcactgtt catcagcaat
     1921 tgtatcaatc taacacgcct atttcaatta caattggcga cagttagatt accagactta
     1981 ttgttcatac agtcaaaaaa gaaagagaag agcaacgagc cttctcccct agaaatctaa
     2041 atacacaatt aaaaatgtag ctttagaaga ctagtctctt ctaaaaatat cacgcgtata
     2101 cactttatct aatacatcat ccaagacaga gtcgtatcta tttgcaacaa ttgcgtgact
     2161 tttagattta aactgattca aattattaat cacttttgaa ccaaagaagg tggaaccatt
     2221 ctctaaagtc ggttcaaaaa taatcacttt tacccctttt gcttttagac gtttcataac
     2281 tccctgaata gaactttgac ggaaattatc agaattactc ttcattgtca agcgatacac
     2341 accgatgatt acttcttttt ctcccaattg agagtattga tcatgtgacg aataatcata
     2401 ataaccagct atcttcaaaa tttgatcagc aataaaatcc ttacgagtcc tatttgactc
     2461 tacaatagct gtcatcatgt tctgcggaat atcattgtaa ttagccaata actgctttgt
     2521 atcttttggc aagcaataac caccatatcc gaaagaggga ttattataat gattaccgat
     2581 acgaggatcc aaaccaactc ctcgaataat cgattctgta tctaatcctt ttagctccgc
     2641 ataggtatcc aattcattga agtaagaaac acgcaaagct aaataagtat tagcaaacag
     2701 cttgactgct tctgcttctg tagcacccat gtgtaatact tcgacatttt cttttagagc
     2761 accttgttgt aaaagttttg caaaaagctt cgccccctca ataactgttt gtggtgaatt
     2821 actctcataa gatacaatga tgcgcgaggg ataaagattg tcataaagtg ctttggattc
     2881 ccgtaaaaat tctgggctaa aaataatatt ttccgtacca aatttttcac gcatagattg
     2941 ggtaaaacca acaggaaccg ttgatttgat aaccattaaa gcttgagggt tgaccgataa
     3001 taccatttgt ataactgctt cgacatgact tgtatcaaag tagttcttag cactatcata
     3061 gttagttgga gcagcgatta caacaatttc tgcatctttg taggcttttt ctgcatccaa
     3121 cgttgcaact agatttaagt ctttttcagc tagatatttt tcaatataat catcctgaat
     3181 cggagacttc ttagaattga ttaaatcaac tttttctttg ataacatcca cagctgtaac
     3241 ttgataatgc tgtgccaata agacagcgat agatagaccg acataaccgg ttcctgcgac
     3301 agcaattttc attagaacta ctccattaat aatctttaat aaactcaata acagacaaaa
     3361 tcgtacaagt aatggttaaa tttccgtgta acctttttct aaaatatctg ctattcgaca
     3421 agaagtttta ccatcaccat atggattaac agcctgagcc atgttactat aatactttgt
     3481 attatctagt aattccttac agtttctata aatcacttcc tcatctgttc caactaattt
     3541 aagagtacca gcctcaattc cctctggacg ttccgtggta tctcgcatta caaggactgg
     3601 ttttcccaat gatggtgcct cttcctgaat tcctcctgaa tcagttaaaa tcaaatacga
     3661 attggataat aaattatgga attcaattac ctccattggt tcaataattc gtatacgatt
     3721 gcaaccactc aatacctcat ctgctatttg acgcactaac ggattcatgt gaattggata
     3781 tactgcctta acatcagtat actcatctat gatacgacga attgctctaa acatctgata
     3841 cattggttcc cccaaatttt ctctacgatg agcagtaatc acaattagac gcgaatcttg
     3901 tgcccaatca agaacctcat gcttaaaatt tgattgaaca gttgtactta aggcatcaat
     3961 agctgtattg cctgtaatgt atatgttttc tatatttttt ccttctctaa tcaaattatt
     4021 agccgaacct tgtgttggag caaaatgata gtttgcaatt atcccaacac tctgtctatt
     4081 aaactcctct ggataaggag aataagtatc gtaggttcta agacctgctt caacatgacc
     4141 tacttgaatt tgtttataaa aacaagccaa tgctgtcgca aaagtagttg aagtatcccc
     4201 atgaactaaa actatgtccg gttcgacctc atctaaaata acttctattt tactaagtat
     4261 actcgatgtt acatcaaaaa gtgtttgacg ctctttcatt atggataaat cataatcggg
     4321 tactacgtta aatacatcaa gcacctgatc caacatttga cgatgttgac cagttacaca
     4381 aacaatagtt tcaattcctt ttcgacgttt tagttcattt acaattggac acatttttat
     4441 agcttctgga cgtgttccaa ataccagcat tacttttttc atctatctac tccttatttt
     4501 tttaaaccta ttgttgcaaa gattttccac atttctttat tcttacgaaa aataagtatt
     4561 gagaagtaac taaatactga aagaaaaatt ttaaccatca gtgtaagtaa aatattgaac
     4621 tcaaaataag aaaaaacaac atgatttact ataaacataa gcaaaatccc taatattgca
     4681 tcaagcaaat ttctttttac ttcaacagca acaataactt ttttccattc cttaaatgaa
     4741 actaatacaa tcataaaaac actaaattct gctaaaacag tggtgaaagc agcaccattt
     4801 tcatggaata atggtatcat tattaagttt aaaatcgcat ttactaccgc agcagtcacc
     4861 gttgaataaa cattgaattt ctctctacct aatggaatat ttaaacaatt cgtcaacact
     4921 ccaccaaaaa ttgcaaatat caaagataga ctcaaaattt taagagtaac cgaagcagga
     4981 gcgtacgcac tcccatacaa aatatacacc acctctcctg ccacaataaa taaccctgag
     5041 gcgattggta gtaaaagtaa tataagccaa ttaatgatgc ttgtaaacaa ttttttaaaa
     5101 ttaacaaaat cattgttact tgcatagaag gacaaacgtg caataacaac aatgtaaaga
     5161 gcggctagta tactctttaa aatagaataa atttttacag ctgaagaata catcccaaca
     5221 ctataagtcc cgcccatact acctaacatc gtcacatcta tattaacgta aattgataca
     5281 gcaagattat taacaaaaaa aaccaaggaa gatttgaaat gtctcactat atctctcgta
     5341 aaaatcaatt ttggagtaaa atactttcga caatggattt gattaaaaac tgctataaaa
     5401 attgttgaaa caacaccaat aagagaatat aaatagtaat cattaggact ctttaccaaa
     5461 gtaaaagtta gaataaagga aaaaatatat actataattg ttctaagagt tatagaaaaa
     5521 taatcttcat aaataatatt aacccactct atccctaacg ttgtgaatag aattgtcgaa
     5581 ctctggagta atattaataa tctatattct tttagcaagg gactaaaata taaaaccaat
     5641 aacaatacta cataagcgaa cagggtggtt atcatttgta acgtgaaaac ctcactccca
     5701 agtctttgaa acttcctacg atcttctcga aatctagctc cttctctttc tacatatata
     5761 ttaacaccta acaatgaaag taaagaaaaa taactaacta ttgaaagtcc aaaatttatt
     5821 tttccgacat tctctactcc taatactctg gatacataag gcagagttgc taactggaaa
     5881 aaaatggcta aacagctcct aattatattt aagcttgcat tcaatttgat tgatttgtta
     5941 atcataatga cctaaaactc atcttcctct tttagctagc tttctataca tgactttata
     6001 tgtaatatcg attactggat taagaagata tactattcta cctaaaagtg taactttttt
     6061 tacacgtact ccatttttag cactatcttt tctaacacaa tcccaatatc tccaccaggc
     6121 cactatttgc aatggaaagc ttaatttcgc ttctggaata ataatatcta aacgtgaaac
     6181 atagtgttga aggaaaccga gataatgctt atccttaaaa tacacatcag tagtcattcc
     6241 attctctaaa tactccttca tcataacaat ttcatttgta cataccaatg aataatttaa
     6301 accaacttgg tcaaatagcc atgcttctgg cacaaatttc atattttctg ctattggaaa
     6361 acgatactgt tgtaatatga cagtcttatt acattcacaa caatctaaaa agcttttttt
     6421 gcgaccaata ccaaaatgaa aatctatata agacatacta agatactcgt caggaattaa
     6481 ttctccaatc atttcacgac gattactatc aaatgcccta gctaatactc cacaatattg
     6541 attatcatcc acaatatctt tagcataatt atatagaata cgtaaccctt catcaatcaa
     6601 ccaatcatct gagtctagaa tcaaaaaaaa tgtccctttg gcattatcta agccaacatt
     6661 gattgctgaa tgctttccac cattttcttt gtaaaaatat cgacaacttt gttctccaga
     6721 ccaatttttt actacacttt cagtatcatc ctcagaaccg tcatcaataa ctaatatctc
     6781 aaaatcttta aatgtctgct ttcttagact ctctaaggtt ctaatcaatg ttttagaacg
     6841 attaaaggtc ggtatgcata cagtaaaaaa catcttattc tccttcttca atataagata
     6901 aaagtctatc tacaacagaa tcccaagaat acttattaag atatttatct ttttcatctc
     6961 taacaatttt tttcatttct tcattgaaaa tagctctctc catcaactca ctaaaaatat
     7021 cacaatcatt gccatctatc agataaccat ttactccatc ttcaataaaa taaggcattt
     7081 cacaaatatt tctaccgata catggcaatc caaaacaaag tgcttcacca aataccaatc
     7141 cataagcttc aaattctgaa ggcaagcaaa aaatgtcaca tttattaaaa tactcaacaa
     7201 gctgtatttt gtttaaatca cccaaccaat gtatattttt aaactctgta ccataaatat
     7261 tctgagtacc agctatatat agctccagat agggatattt tttgtgtaat tttacaaaag
     7321 attttactac taaatctcca ccttttctat aaaaatcacg tccaacgaat aaaaatttat
     7381 tacctacttt caatgaagag tcaatatctt ttttgtctaa attaatccca ccaccaacac
     7441 aataaacatt gtctatacct gctgatttct ttatttcttc tgctacccaa tgagacatcg
     7501 taaaaattgc cttagaatgt ctatacactt cgttctgtct cttcactttt tcttgtatac
     7561 tttggaaact cgcatttgaa aaattacttt tttcaaatgc tttagaatat tttaaataaa
     7621 tcatttctaa agcagaagca cttaaatctt cataaatata actattttca gctttaccta
     7681 tacaaccaat ttgcaaacta ggaaaagagg tgtctaatgc ttttaatttt ccctgatcga
     7741 ttctatcaat aacagcatta aattgattag ctttaccaat tccattatat gaaaaagaaa
     7801 tcttagataa tctcattagt aacttttcca atgtagtata tcgaatatcg tattcattaa
     7861 tattgcactt tttttccaga gctttgtaaa gtgaccaggt agtgcctgac caggtcttct
     7921 ttctattcaa atcccattgg caaacataat tcaatctcat attagttttt ccacaaaatc
     7981 atgatttttc tttctgctat aaaatagata actatacatt ataaccaatg tataattcca
     8041 aaaatacaag aacgtaccct gaccaaataa atcatataat gtcagagtta gtagtgaaga
     8101 aaataagacc tcaaacataa aatcattttt cactccataa gtaattgcac gattttttat
     8161 atccttaaaa atacgaaaaa ttaaaaagta agtcaagaat aaaccaataa ttcccaattc
     8221 tgaagcgtat tcaagaaaaa aattatgggt acttaatcca actactcttt ccgtgacaag
     8281 cctactgcta aaattttgta actgggtagc agattgcctt gtaaacattc ctgaaccaat
     8341 accaaaaaat ggagatgaaa aaaatagtcc aattgatgca tcccacagca catatcgagt
     8401 gtgcattgat gaatctatat ttgctgttaa actccaaaaa atttcattgt acttatagat
     8461 atagtattct ttcaatgaat ctggaatcgc aaataaaata ataaaagcaa taataataaa
     8521 actaaaaata attttaggta aaagcttcag caagtttctc ttagacaaaa gtaagtatat
     8581 aaaaatattc aacagaatta atataattag cgttctttgt tttgacacta tcgctccagc
     8641 tataccaatt agggaaatta gttgagattt cttttcatca aatttccttc ctctcttaaa
     8701 taaaccataa agaatatatg gaagagcaaa acatgttatt gccggaccaa tgaagccacc
     8761 agctctagct gtaccattaa aaaataattc tataatcgca tatatattat taataactaa
     8821 caaaatcact ccgatattcc atattttttc aaaagagact ttcgtctgaa aattcataat
     8881 tatgaaccac actaagataa attcaaaaaa atatcccaaa ctcatagcag tccccaaaga
     8941 taaatttccg aaaaatccca acgttagcat tacatatgct atccgaatga taatcaaaaa
     9001 tgaaatgtcg cgtataaggg taacctttcc tctacaaaat attaaacaat agctaaaaag
     9061 aagaaaaagg aggacatcaa caagtctaac tggacctaat atgttaaatt gataggtatt
     9121 ctgtaaaatc agagaaagta ataattctaa catgcactct aagttcctct acaaagttct
     9181 atataatagt ttaataattc cttacatgct ttttggtatt cgttattctt tatgcatttt
     9241 aataataatg aaaagtaatt caataagaat aaaactgata actggaaatt attaatctca
     9301 caatattttt tcagaacata catggaactt ttgtatcgat ttaaataata tgttgttcct
     9361 tgagtaccaa tagtaataga gccaaagtga ataatttttt gatcagttag taagagtgct
     9421 tgtaactctg ctttatataa cttatgtgca attgcatcct cttcataaaa caaaaatata
     9481 ttctcatcca ataggccaat ttttgaaatc gtaagcttac taagtaagaa acaacaaccc
     9541 gataccatgc catcaaaata atatttttct ctagaaatag tattttgtgg aaaacgataa
     9601 aagtaagcaa atggcttctt agaaaataaa tgactataat aagtcattcg atgcttataa
     9661 ctcttttgtt caccattatc cgtaccaata atcaatgttc cgactactcc caatttcggt
     9721 tctgtttggt ttgcaataat catattatca atcgttcccg agggaaaaat caaatcatta
     9781 ttacataata aaaagtaccc acatccgtcc ttaagagctt gctctatacc tctattcaat
     9841 cctgctgaat accctttact ttcatttacc ttaatcaatg tcacatcatg attatcacgg
     9901 tatctattcc aaagcatatc gaaagaatta ttagaagacc cattgtccac aatataaatt
     9961 ttatgttcat aattaaccgg ataatcaaac aaactctgga cacaattaaa agtattttga
    10021 taactattat aatttacaat tactatccct agcatatatc ccccttacat atttaaaatt
    10081 aaatcaactt ctttcattat ggtattctga gcaaaaaatc tactatcctt tataatattt
    10141 ctactattat ttaatacaaa atcaagactt gattctttga catcattatc aattaaatat
    10201 acattctcaa aatcagcaaa aacttcatca caaacaggaa tattggatgt aataactgga
    10261 atacctagtg ccgccatttc acacatcatc aatccttgag catcaacttt agttggcata
    10321 aacgcaaatt tacaatatga caatatttct acgatttcag tatgcgataa gttgtgattt
    10381 ctccactcaa tatttgaact tttttcaaaa tgtgtaaaat attccccttt tccaataacc
    10441 aaaaaacgag cttttggggt atttttagca tactgattta caatatctat accatatttc
    10501 gagccatcca aattattcct aatcgtaaca aaatcatact taaaatctga gacatctgaa
    10561 atgatatttt tttcaaattc ctcccctata caattgtata tgatacttga agaataatct
    10621 aataatccat agggtaattt tgtccattta taaaattcat ctttcatcca ctggctcaca
    10681 aatacaaagt gagatttata tgcaatttta ggcaaatatt ttttccaaat aaataattta
    10741 attaaatcat atctatcctg ataaaattct ctaaacctat ctcgagatat aaacggatat
    10801 ggagcaggat aggacttggt aattctcatt acttcatgtc catggaaaaa aaataaaaac
    10861 ttttcaaact ttgagccaaa tttccgtata aaaaggtagt ggtgcttaat attagccgca
    10921 tgaatcacta agatgtcata atttttatta atcttatgat aatctttctg tgtgataacc
    10981 gatataccat caatatcata ggatttcttt gcgctaaaat tgagaactgt cacttcaatt
    11041 cccatttgtt tataataagt atttctagta tgaatgtaca tcaatgttac accgccattt
    11101 ttattcgggt aatcttctgc tagtaccaaa atcttcttac tcataaccct tccccttctt
    11161 tttaactaaa tatcgattcg caatttcatt acaaatttga acaattgttt attcttttgt
    11221 aaatgggaat attataccaa agtataaaaa taaattttgg aatcgcacgg ttaatcttgt
    11281 cctgtatttg acaataaggc tatctatata ccgtagaaaa tagcttcaat aatgttcatt
    11341 ttaaccgaaa aattttgatg atcccccaaa ataaaatatg aaatacactt ctaactatgc
    11401 ttaggtgttc tatatcccta tataatttcc aatgatactt gattaaatca actttattac
    11461 ttgaaataga atttttccta attcgatatt gcattaaaac atctggtata ccatgaatat
    11521 aaggctctac ctttagcatt tttaaccaca gtgcatcatc atttctttta cgtatgttcg
    11581 gcacctcaaa ttttcccatt ttcgatacat tatacataac agttgagttt ccgactggac
    11641 aatccaataa tagtcgatta taactaactt ttttaactga tttaacaatt ttacctagag
    11701 gaaaatcagt ttcatcaatt tgctcatagg aggtgcatga aaaaaacacg ttattttttt
    11761 ccataaattc caactgctta gttaacttag agggaatcca taaatcgtct gaatcacaaa
    11821 aagcaataaa ttcacctttt gatcttttca tagcctctgt tcgagctact gccgcacctg
    11881 aattttcagg taaaagatga tacttgattc ggctatccga ttcaacataa ccatcaacta
    11941 tatcttttgt attatcggtt gattgatcat caacaataat taattcccaa ttctgatatg
    12001 tttgcgccaa aattgattct atggttttgg aaatatatcg cccacaatta tatgtcggca
    12061 tcacaatcga aactaaacct ttttccatta ttatttaaag aacctcaaaa ataatccttt
    12121 tcaaactctc tatcaaatac tctacatctt catcactcaa gttggtatga agaggaagtg
    12181 taacttcatt ttcaaaatac tggtaggcat tcggataatc tttcatatca aaaccaagat
    12241 tcttataggc tgtgagaaga ggcaatggtt tgtagtgaac attgcaagca atccctgctt
    12301 cagccatttt ttgaatcact tcatttcgtt gttccaaagt aaagccttcc agatgtgtga
    12361 tatacaagtg catagaggat tgcttatctt cagtctgatg taccaacggc ttaatcgaag
    12421 ttccctcaaa acctgcattg tatttctcaa tgatttctct gcgacgattc aacagagatg
    12481 ggtaacgttc taattgcacc agaccaatac ctgccataat atctgtcata ttacacttgt
    12541 aaccaggaat aacaatgtca tattcccatg accctaattg tgtcttagct aatgcatcct
    12601 ttgtctgacc atgaagagag taaatctgaa actctttata catctcttca tcatccaaat
    12661 caggatgtgg tctccatgtc acactacctc cttcagcagt tgtaaaattc ttaactgcat
    12721 ggaaagaaaa tgaggtaaaa tctgctaggg aacccgctgg tttcccctta taactagcac
    12781 ctagtgagtg tgcactatca gataggataa taactcgccc gaaaagtttc tgccatttat
    12841 tatcagaagc aacatacaaa gagcgtttgt tttctacgat ggtataaatc ttatcataat
    12901 cacaaggaat accagctaga tcaacaggaa tgataacttt tgttttcgga gtaatcgctt
    12961 tttccaaagc atcatattcc atctcaaagc tgtttttttg aatatcaacc atcacaggag
    13021 ttgctcctac atgagtaatg acactacatg aggcagtata ggtcatagca ggaacaataa
    13081 cttcatctcc gggtccaaca ccaagaattc gtaagactag ttccaatcct gcagtagcag
    13141 aatttaaaca cacattttta ttggttcctg taaatactga taaccgacgc tctaactctt
    13201 ttgtcttagg ccccgttgta atccagccag atttcaatgt atcaattact tcattaattt
    13261 cagcttgagt aatatctggt ggtgaaaatg gaatatttgg tctttttgtc attttaagct
    13321 cctattttac tacattaatt acggtgtcta tcattacctt aaaatcagaa attattccaa
    13381 agtttttgat gtactccaaa ttatatttca ttttttctgg taacaccttt tgaacatagg
    13441 cctcatctgg actatagcct tgagaacaat attcttctaa aacactatct tcatccttat
    13501 atgcaatact tgctggtgaa gtaattcctg ctggcaacag caaggtcgca agcatttcat
    13561 cagtataatg atccacatac ttttgtactt ctggtcttac accaacaaaa ctcatatctc
    13621 ctgtcagaac attaaagagt tgaggcactt catccagcct atattttctg ataatatgac
    13681 cgacttttgt aatacgatta tcttgaccga ctgtgactag actaccgacc ttatccgcat
    13741 ctgtaaccat ggttcgaaac ttaaaaattc taaacattcg accatatctg gtaaccctct
    13801 cttgacggta aaaaacggat cccttgctgt ctatttttat ccaaatagat aaagcaatga
    13861 tgactggtga aagtaatact aataggatta gtgctaataa cctatcagta gcatatttta
    13921 aaaataactc gaactttctt gactcaattt tatcaaaata ctgacctgtt atttcattcg
    13981 cttttagata ttcttttgtt atagttcttc cgctatgcat tcattctcct atttgcaagg
    14041 ttagtggttt tgtcactaat cctcaatcct aaaataatct atatagtgct taaatcgatc
    14101 ttgtgcggct aagcaagtat ctagcaagaa accattctgt tctccccatt cagctaagcc
    14161 acgatagaaa aaatacttca tgtcatctcc aatgataaag gggacatatc catgtttgag
    14221 acattctttg aacatgatga gtcggccaac tcgcccatta ccgtcttgaa aggggtggat
    14281 acgttcaaat cgaacatgaa aatcaagaat atcttcaaag gaagtttgtg ataaactatg
    14341 atagcttctg agtaattctt gcatatccaa acctacattc tcaggtaatg tggtttcctg
    14401 acctcctact tcatttggaa gtttcttgta ctctccgact tgaaaccaat ctttccgaga
    14461 atcagacgtc cctctcttca atatagcatg gacatccttt atccagattt cattcaagtc
    14521 atcttgaaaa ctgtccaaaa gaaaatcgaa tgcggtaaaa tgattagcgg tctcgataat
    14581 atcatcgaca ctgatagttg aactattctc aaaaccaatc gtgttggttt caaaaatata
    14641 cctggtttga tcatgggtca gctggctacc ctctatatgg tttgaattaa aagccagctc
    14701 tatctgaacc ttatgataaa gccccccctt cattctatct tctttttctc ttagtaacct
    14761 atggccgagc atatttataa tttattcctt tcttttcgtt caagacacta aacaggatga
    14821 atggttttat gccccttgtt ttgttctgca agagatggaa gaaagaagaa ctatcatgac
    14881 aacaaatcac atggtagaaa gtatgtgagt ctggagtctc tgctacttcg ccgacatata
    14941 catatgatcc ctcatatcaa ataaatagac ttattttcat actaaacaat tcgttttgaa
    15001 aagggaagag ttgtcaaaag atagatacat cttaaatttt atctgactga atccattctg
    15061 ttaatattat cctcaattac tggagcattg atttatagaa cacatttcac acagtagttt
    15121 ttgcaatggt caataaaatt ctatcatgcc cactctagtt cttcataact atcaaataac
    15181 tcggtgattt ctttcactat cacatgaaac acacttcact tcaggtaagt gagaaaaagc
    15241 gccattaaga ctgtctgaat tggtctgatc tcttaatcgc aagtgttcct tccaatatct
    15301 gcgggaaaag tgttgaagct attgtaaaaa aatattattg acaattcttt accttagcat
    15361 ttagccagat aatcaaacta tctatttaag gatacgattc aagttaccaa aaatacagat
    15421 tcacaaccac ttacttggaa ggattaagaa cgcaaaacat gttttttgta gacaaaaaac
    15481 ttagagaaac cttgattatg tcagctgcag tttttataaa acttcttgat ttttatcaac
    15541 agtcttggta gaaaatctgg tccttgactt taatactatt aaacaagtat atacaataaa
    15601 aagtttccca ttta
//
LOCUS       DATUCG010000051        15573 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.052, whole genome shotgun sequence.
ACCESSION   DATUCG010000051 DATUCG010000000
VERSION     DATUCG010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15573
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.052"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            53..1111
                     /locus_tag="VCA20_001238"
     CDS             53..1111
                     /locus_tag="VCA20_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding domain-containing protein"
                     /protein_id="HEP1835064.1"
                     /translation="MKTNLKKLLYSGVTLMSLGVLAACSSTSSSTATSSSAATSQTSV
                     ATTSNNSTSSDSSTSSIDWSALPTTEVTLSNDGLKITEGGNYILTGSTTAGVTVETDA
                     NVRIILAGTDISSSDTAAINVINADNVELEIKDGTMNTVKDTSNHTDINIEGAIHVEA
                     DLTITGNGSLTVEGNFQDGIVSTDDLVVNSGNITVTAVDDGIRVKDSLTINGGTINVT
                     AGGDGIKAETALTIDGGTITVSESAEALEGANITVNGGTLDVYGSDDAINAASTTSSE
                     IFIKVTGGDLKVAVGSGDTDAFDANGDIYISGGTIDVTAQSTFDFNGTSELTGGTVIV
                     NGEQITQITAIGPGHGGW"
     gene            complement(1147..1806)
                     /locus_tag="VCA20_001239"
     CDS             complement(1147..1806)
                     /locus_tag="VCA20_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HEP1835065.1"
                     /translation="MEFDLFLTICNHIGTIAFAVSGAVKGFKKKLDIFGISLLGIITA
                     VGGGIIRDTMVNRIPAALTDPMAIYLSLGVAISMYLIVINLKQDKPLDKKMIHFLSQT
                     NLIFDAIGLAIFALIGASTGISLQLNALTSGILAALTGVGGGIARDLLVNETPIVLKE
                     DVYAALAFFSGVLYHLCVVNWKLPQIPTFITIFIISLIIRLLVIKYKINLPNMESKRK
                     V"
     gene            2039..3007
                     /locus_tag="VCA20_001240"
     CDS             2039..3007
                     /locus_tag="VCA20_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEP1835066.1"
                     /translation="MVSITKEQHLDMFLKMQQIRDVDMKLNKLVRRGFVQGMTHFSVG
                     EEAAAVGPIAGLTDQDIIFSHHRGHGHVIAKGIDINGMMAELAGKATGTSKGRGGSMH
                     LANVEKGNFGSNGIVGGGYALAVGAALTQQYLGTDNIVIAFSGDSATNEGSFHESMNL
                     AAVWNLPVIFFITNNRYGISTDISYSTKIPHLYQRAAAYGMPGHYVEDGNDVIAVYEK
                     MQEVIEYVRAGNGPAMVEVESYRWFGHSTADAGVYRTKEEVNEWKAKDPLKKYRKYLT
                     ENKIATDEELDAIEAQVAEQVEASVKFAQESPDPDISVAYEDVFVD"
     gene            3269..4264
                     /locus_tag="VCA20_001241"
     CDS             3269..4264
                     /locus_tag="VCA20_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEP1835067.1"
                     /translation="MAETKVMALREAINLAQSEEMRKDEKVFLMGEDVGIYGGDFGTS
                     VGMLDEFGPKRVRDTPISEAAIAGSAVGAAQTGLRPIVDLTFMDFITIALDAIVNQAA
                     KTNYMFGGGLKTPVTFRVASGSGIGSAAQHSQSLEAWLTHIPGIKVVAPGTANDAKGL
                     LKSSILDNNPVIFLEPKALYGKKEEVNLDPDFYIPLGKGEIKREGTDVTIISYGRMLE
                     RALKAAEEVAAEGISVEVVDPRTLIPLDKELIIESVKKTGKVILVNDAYKTGGFIGEI
                     ASIITESEAFDYLDAPIIRIASDDVPVPYANILENAVLPNVEKIKAAIYKQVNKG"
     gene            4605..5993
                     /locus_tag="VCA20_001242"
     CDS             4605..5993
                     /locus_tag="VCA20_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002885106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HEP1835068.1"
                     /translation="MAIEIIMPKLGVDMQEGEIIEWKKQEGDFVNEGDVILEMMSDKT
                     SMELEAEESGVLLKIVHGNGATVPVTEVIAYIGAEGETVEAGASSAPAVEPAAAIEEV
                     PAGRTPVIVAPATAAKPQGGGKVRATPAARKLARELGIDLGLVPGTGANGRVHKVDVE
                     DFKGVAPKATPLAARIAADQGVDLSTLTGSGVNGKIVKNDVLAVLAPAAVETVAPAPK
                     AEEKPAKELPEGVEIIKMSPMRKAISKGMVNSYLTAPTFTLNYDIDMTNLMALRKQVL
                     EPIMNKTGLKVTFTDLIGLAVVRTLMKEEHRYMNASLINDAQEIELHKFVNLGIAVGL
                     DDGLVVPVVHGADKMSLSDFVVASKDVIKKAQSGKLKGAEMSGSTFSITNLGMFGTKT
                     FNPIINQPNSAILGVASTVQTPVVIDGEIKIRPIMALCLTIDHRIIDGMNGAKFMVDL
                     KNLLENPLELLI"
     gene            6263..8023
                     /gene="lpdA"
                     /locus_tag="VCA20_001243"
     CDS             6263..8023
                     /gene="lpdA"
                     /locus_tag="VCA20_001243"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEP1835069.1"
                     /translation="MAIEIIMPKLGVDMQEGEIIEWKKQEGDFVNEGDVILEMMSDKT
                     SMELEAEESGVLLKIVHGNGATVPVTEVIAYLGAEGESVEVGSAPAPAEVAQATADLK
                     AAGLEVPAAPATAPQAPKAELAADEYDMVVVGGGPAGYYAAIRGAQLGGKIAIVEKSE
                     FGGTCLNKGCIPTKTYLKNAEILDGLKIAAERGINLASTNYTVDMDKTVDFKNKVVKT
                     LTGGVQGLLKANKVTIFNGLGQVNPDKTVVIGDKVIKGRSIILATGSKVSRINIPGID
                     SKLVLTSDDILDLREIPKSLTVMGGGVVGVELGLVYASYGTEVTVVEMADRIIPGMDR
                     EVSVELQKVLSKKGMKFLTSVGVSEIVEANNQLTIKLNDGSEIVSEKALLSIGRVPQL
                     AGLENLNLELDRGRIKVNEYQETSIPGIYAPGDVNGTKMLAHAAYRMGEVAAENAIHG
                     NHHKAKLDFTPAAVYTHPEIAMVGLTEDQAIEKYGKENILIGRNSFTGNGRAIASNEA
                     HGFVKVIADKKYYEILGVHIIGPVAAEMINEAATIMESELTVDDVAASIHGHPTFSEV
                     MYEAFLDVLGVAIHNPPKRK"
     gene            8164..9009
                     /locus_tag="VCA20_001244"
     CDS             8164..9009
                     /locus_tag="VCA20_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEP1835070.1"
                     /translation="MRKIIFLDVDGTLVDYHNRIPESAIRAIRQARENGHLVYVCTGR
                     SRAEMQPELWEIGLDGMIGGNGSYVEHQGQMVMHQVISKVDSRAIVDWLHERGLEFYL
                     ESNNGLFASENFRERARETLRIYSMNKGKTAEEVANQEVEDVIHGMVFDGELYRDDLN
                     KVSFVLDSYQDHLDSKEAFPQLVANTWGGRGETALFGDLGVKDIDKAHAISVLLDHLG
                     ASQADTIAFGDAKIDISMLDYCAVGVAMGNGGAEILAMADMITDDVEEDGLYNAFERL
                     GLLDK"
     gene            9189..10022
                     /locus_tag="VCA20_001245"
     CDS             9189..10022
                     /locus_tag="VCA20_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEP1835071.1"
                     /translation="MQIEKVYNNNVIQAIDQQGRELIVMGKGLGFQKKAGEELDTSKI
                     EKTFVLQNDYQQSDLSSLYLQMESSEVEVVNAIINRAEETLEVQFDLSLYLALADHLH
                     FVFQRCREGIFIENPLSWEVRKFYPKEYQIGLEALQLVQERLGLELGKGEASSIALHL
                     VNAQKNGAFGKETQTISKIVTQILDIVRLHFGSVTYEEDTSYHRFVTHIQYFAQRVAN
                     GVVEGVNDAFLYEQVMANYPDSFACTTKIRQHIAEVYHFEMSKDEQVYLTIHIQRLKT
                     S"
     gene            10232..12141
                     /locus_tag="VCA20_001246"
                     /pseudo
     CDS             10232..12141
                     /locus_tag="VCA20_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002266487.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
     gene            complement(12188..12517)
                     /locus_tag="VCA20_001247"
     CDS             complement(12188..12517)
                     /locus_tag="VCA20_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEP1835072.1"
                     /translation="MITMHLYYTGPADNARAFSQEMEASGIADRIRKQVGNTRYQYFA
                     PLDDPHSILLVDSWIDQAALDIHHASPMMQEILDLRSKYQLTVTAERYQSDEAGIPEQ
                     DKDFLQR"
     gene            complement(12767..13681)
                     /locus_tag="VCA20_001248"
     CDS             complement(12767..13681)
                     /locus_tag="VCA20_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEP1835073.1"
                     /translation="MQPPLTDKQFKHTIDYDSRLLPYKYYHTSVIDGIPDILFHWHNE
                     FEINHVYGGTARYHIDYDYFNSQAGDIILIRPNAAHSIHPIEQRGHETDTLLFHLDML
                     GASHLDQTSMLYLQPLQTDVFKCIPRIQPQDPGYAEIRQCLLTIFELVRHQPPYFELP
                     LKSRLFDFFYLLYRYRYIVRKNTDDMYRKNEKLRLLIEYIQQHYAENLTIEQMAEQMG
                     YTKTHFMTIFKQQTGTSCMEFIIQVRLRAATELLTNTLKPVLEIATEVGFNNLSNFNR
                     QFKQYYHTTPSSYRKQLRKNRTYTNFDK"
     gene            13793..15274
                     /locus_tag="VCA20_001249"
     CDS             13793..15274
                     /locus_tag="VCA20_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEP1835074.1"
                     /translation="MSRFPKNFLWGGAIAANQAEGAYNVDGRGLVQTDVTTGGSVNSP
                     RYTTYIDKDGKPGKYASMGHAGGHLPEGATYAVLEDHYYPNHKAVDFYHRYKEDIKLF
                     AEMGYSVFRLSISWARIFPNGDDAKPNQAGLDFYRSVFEECRKYGIEPLVSIWHFDTP
                     LSLEQRYGGWTNRKLIDFYVRYSETIFKEYKGLVKYWITFNEINNTIMFLDMFSEPKD
                     SDYQDAYQHLHHQFVASAKAVKLGHEIDPNYRFGNMICGITFYTGTCDPADILENQHK
                     WEQNIFYCSDAQAKGRYGSYANRLWKEHNVVLQMEEGDLEILAEGKVDMYTFSYYMST
                     VVTTHKVEDTVSGNFSIGAKNPYLTYSDWGWAQDPTGLQYYLEKIYDRYEIPMMIVEN
                     GLGALDTVEEDGSIHDDYRIDYHRAHVNAMADAIANGVDLIGYTTWGCIDLVSAGTGE
                     MRKRYGFIYVDMDDAGNGTLARTPKDSFYWYKKVIASNGEDVE"
BASE COUNT         4613 a         2943 c         3640 g         4377 t
ORIGIN      
        1 agcatatcag gaagggatac gacttcctaa agaaagaaaa ggaatctaca ttatgaaaac
       61 aaatcttaaa aaattacttt actctggtgt tactcttatg agccttgggg tcttagcagc
      121 atgttcatct acttcaagtt caacggctac ttcaagctca gcagcaacta gtcagacaag
      181 tgtagcgaca acttcaaata atagcacttc atcagattca agcacgtcat ctattgactg
      241 gtcagccctt ccaacgacag aagtgacgct ttcaaatgat ggcttgaaaa ttactgaggg
      301 tggaaactat attttgactg gttcaacaac agctggtgta acggttgaaa cagatgccaa
      361 tgttcgcatc attttagcag gtactgacat ttcaagttca gatactgcag caatcaatgt
      421 tattaatgct gacaatgttg aattggaaat caaagatggt acgatgaata cagtgaaaga
      481 taccagcaat catacagaca tcaatattga aggggctatc catgttgaag ctgatttgac
      541 cattacagga aatggtagcc tgaccgttga aggcaacttc caagacggca tcgtatcgac
      601 agatgacctt gtcgtgaatt caggaaatat taccgtcact gcagtggatg atggtatccg
      661 tgttaaggat tcgttgacaa tcaatggagg tacaatcaat gtaacagcag gtggtgatgg
      721 tattaaagcg gagacggctt tgacaattga cggaggtacc attacagtat cagaatctgc
      781 tgaggcactt gaaggtgcta acattacggt aaatggtggt acattagatg tttacggttc
      841 agatgatgct attaatgcgg caagtacaac gtcatcagaa atcttcatca aggtaactgg
      901 cggtgacttg aaagtagctg taggtagtgg agatacggat gcctttgatg ctaatggaga
      961 catttatata tctggtggta caatcgatgt aacagctcaa tctacttttg acttcaacgg
     1021 aacttctgaa ttgacaggag gtactgtgat agttaacggt gagcagatta ctcaaatcac
     1081 agcaatagga ccaggtcacg gtggctggta atcaataata atacaaaaag gtctgagttg
     1141 acgaactcag acctttcgtt tgctttccat attgggaaga ttgattttgt atttaattac
     1201 caataatcga atgattaaag aaatgataaa aatagtgatg aaggttggaa tttgtggtaa
     1261 tttccaattt acaacgcaaa gatgatagag gactcctgag aaaaatgcaa gggcagcata
     1321 gacatcttct ttaagaacaa taggtgtttc attgacgagt aaatcacgag cgattccgcc
     1381 tccgacacct gtcaaagctg ccagaatacc actggttaga gcgtttagct ggagagagat
     1441 gccagtactg gctccaatga gggcgaagat ggctagaccg attgcatcaa agataaggtt
     1501 tgtctgggaa aggaaatgaa tcatcttctt atcaagtggt ttatcctgct ttagattgat
     1561 aacaatcaag tacatgctaa tcgctactcc gagagataaa tagatggcca taggatcagt
     1621 caaggcggca ggaatgcgat tgaccatcgt atctcgaata attcccccac caacggcggt
     1681 aatgatgccg agcaagctaa ttccaaaaat atctaacttt ttcttaaatc cttttacggc
     1741 tcccgaaaca gcaaatgcga tagtgcctat gtggttacaa atcgtaagaa ataaatcgaa
     1801 ttccatatga acctccgtac ctatattaac agaaagaaag acggggtgca agcttatttt
     1861 aaagtttgtg aaaaaagttt caaacaagta aagtgaatct tttgaggatt tgtgaaaaaa
     1921 cgtactaaat caaggttttc tcgaaaattt ctgtaaaaag aaaacggttc aaatacttgc
     1981 catttgtgaa atattggtct attatagtac agaaagagaa aaaactttgg aggaaattat
     2041 ggtatctatc acaaaagaac aacatttgga catgttcttg aaaatgcaac aaatccgtga
     2101 tgttgatatg aaattaaaca aattagtgcg tcgcggtttc gtacaaggta tgactcactt
     2161 ttcagttggt gaagaagcgg cagctgttgg tccaatcgct ggtttgacag atcaagacat
     2221 catcttctca caccaccgtg gtcacggtca cgttatcgca aaagggattg acatcaacgg
     2281 catgatggct gagcttgctg gcaaggcaac tggtacatca aaaggccgtg gtggttcgat
     2341 gcacttggcc aacgtggaaa aaggaaactt tggttcaaac ggtatcgtag gtggtggtta
     2401 tgcccttgcg gtgggtgctg cccttacgca acaatacctc ggtacagata acattgttat
     2461 tgccttctca ggtgactcag cgacgaacga aggttcattc cacgaatcaa tgaacttggc
     2521 agctgtttgg aacttgccag ttatcttctt cattacaaac aaccgttacg gtatttcaac
     2581 tgacatttct tattctacta agattcctca cctttaccaa cgtgctgctg catacggtat
     2641 gccaggtcac tatgttgagg atggtaacga cgttattgct gtttatgaaa aaatgcaaga
     2701 agtcatcgaa tatgttcgtg caggaaatgg tcctgccatg gtcgaagttg aatcgtaccg
     2761 ctggttcggt cactcaactg ccgatgcagg tgtttaccgt actaaagaag aagtcaatga
     2821 gtggaaagca aaagacccac ttaagaaata ccgcaaatac ttgacagaga acaagattgc
     2881 gacagacgaa gaattggatg ccatcgaagc acaagtcgca gaacaagtgg aagcatctgt
     2941 gaaattcgca caagaaagcc cagatccaga catctcagta gcgtatgagg atgtgtttgt
     3001 agactagggt gtgaggacgt taaagaggca cagaaaaatt agggagtcag ctagtgatgt
     3061 ggacatcact aatggctcat ctatttttcc aagcctctag tctgaaccca actacaaacg
     3121 taagaagttt ttgactaagc tctaaagagc aagtcaaact agggtagttc gagtagctat
     3181 gacgcttgat tacaatgaag taattgatta gttttctata ttttacttgt tacagtaaag
     3241 ataaattgat ataaattagg agtaaaaaat ggctgaaaca aaagtaatgg ccttgcgtga
     3301 agcgattaac ttggctcaaa gcgaagaaat gcgtaaggat gaaaaagtat tcttgatggg
     3361 tgaagacgtc ggtatctacg gcggtgactt cggtacatct gttggtatgt tggacgagtt
     3421 tggtccaaaa cgtgttcgcg acacgcctat ctctgaggct gcaatcgctg gttctgcggt
     3481 tggtgcggct caaacaggtc ttcgtccaat cgttgacttg acttttatgg acttcatcac
     3541 aattgccctt gatgcaattg ttaaccaggc tgccaaaacc aactacatgt ttggtggtgg
     3601 tttgaaaaca cctgtaactt tccgtgtggc atctggttct ggtatcggtt cagcagcaca
     3661 gcactcacaa tctcttgaag cttggttgac tcacattcca ggtatcaagg tggttgcacc
     3721 tggtactgct aacgatgcaa aaggtctttt gaaatcatct atccttgaca acaaccccgt
     3781 tatcttcttg gaaccaaaag ctctttacgg taaaaaagaa gaagtgaact tggatcctga
     3841 tttctacatt ccacttggta aaggtgaaat caagcgtgaa ggtactgatg taaccattat
     3901 ttcatacggt cgtatgttgg aacgtgcctt gaaagcggct gaagaagtag ctgcagaagg
     3961 catcagcgta gaagtggttg acccacgtac ccttatccca ttggataaag aattgatcat
     4021 cgaatctgtg aagaaaactg gtaaggttat cttggttaac gatgcttaca aaacaggtgg
     4081 tttcatcggt gaaatcgcat caatcatcac agaaagtgaa gcctttgact acttggatgc
     4141 accaatcatc cgtatcgctt cagacgatgt accagttcct tacgcaaaca ttcttgaaaa
     4201 cgcagtattg ccaaacgtag agaaaatcaa agcggcaatc tacaagcaag taaacaaggg
     4261 ttaattttat cgtcatccgc ttatcttttc tgactgtgtt gatttggctt gcctaacgtc
     4321 agttatgctt gcaccaaatc gtcttgtcag aaaagcaatc ggaaagacga taaccctaat
     4381 atgctagaaa taaaatgttc tagcatgact aagaatgctt ggaaaagaat taagccactt
     4441 cgattatcga agtctggttt ggttcttgac aaggtaagaa actgaaagaa aggaattgac
     4501 cccgtccgga aaactcggaa aaaagataaa tcgttacgaa aatcagaatt ttcgacgatt
     4561 tcctattttt cattcgtttt ctgttcggac ttggtatctt aaaaatggct attgaaatca
     4621 ttatgcctaa gctcggtgtg gacatgcaag aaggtgaaat catcgagtgg aaaaaacaag
     4681 agggcgattt cgtcaacgaa ggtgacgtca tcttggaaat gatgtctgac aagaccagca
     4741 tggagctgga agcagaagag tcaggcgtcc tattgaaaat tgttcacgga aacggtgcca
     4801 ccgttcctgt aacagaagtt attgcctaca tcggtgctga aggtgagacg gttgaagccg
     4861 gcgcttcatc tgctcctgct gttgaaccag cagcggctat tgaagaagta cctgcgggtc
     4921 gtacaccagt aatcgttgct cctgcaactg ctgccaaacc acaaggtggt ggcaaggttc
     4981 gtgcgactcc agcagcacgt aaattggcgc gtgagcttgg aatcgatttg ggtcttgttc
     5041 caggaacagg tgctaacggt cgtgtccaca aggttgacgt cgaagacttc aagggagtag
     5101 ctccaaaagc aactccgctt gcagcccgta ttgcggcaga ccaaggtgtt gacttgtcaa
     5161 cccttacagg ttcaggtgtc aacggtaaga ttgtcaagaa cgacgttctt gcagtacttg
     5221 ctccggctgc tgtagaaact gttgctccag caccaaaagc agaagaaaaa ccagctaaag
     5281 aattgccaga aggcgttgaa atcatcaaga tgagcccaat gcgtaaggcg atttcaaaag
     5341 gtatggtcaa ctcttacttg actgctccga catttacact taactacgat attgacatga
     5401 ctaacctcat ggcacttcgt aagcaagtcc ttgagccaat catgaacaag actggtttga
     5461 aagtaacctt tacagacttg atcggtcttg cggttgttcg tactttgatg aaagaagaac
     5521 accgttacat gaacgcatct ttgattaacg atgcccaaga aatcgaatta cacaagttcg
     5581 tcaaccttgg tatcgcagtg ggtctagatg atggcttggt ggttccagtt gttcacggcg
     5641 cagataagat gagcttgtct gactttgtgg tggcttcgaa agatgtcatc aagaaggctc
     5701 aatctggtaa gttgaagggg gctgaaatgt caggttcgac cttctctatc actaacttgg
     5761 gtatgtttgg tactaaaacc tttaacccaa tcatcaacca accaaactca gcaatccttg
     5821 gtgtagcatc aacagttcaa acaccagttg ttatcgacgg tgaaatcaag atccgtccaa
     5881 tcatggcact ctgcttgacc atcgaccacc gtatcattga tggtatgaat ggtgctaagt
     5941 tcatggttga cttgaagaac ttgctggaaa acccattaga attgttgatc tgagataaga
     6001 tgcaaaaggc gttaagaaaa ccgtatgaaa ataggaaatc aacgatgtgc tttgaggcac
     6061 gaggcgattt atctttttca ataggttttt agcctgtgct caatttggta aagagaaaaa
     6121 ataaagaaag gatgtaggac gagttcgtga catttgaaca cgaactaaat gcttcggaat
     6181 ttagatagtc cgcctcggat gcaagcatcc tacgttggac tcctaaaact ctgtcgcatt
     6241 tgacgttctt tgtatcataa ctatggcaat tgaaattatt atgccgaaac ttggtgtaga
     6301 tatgcaagaa ggcgaaatca tcgagtggaa aaaacaagag ggtgattttg tcaacgaagg
     6361 tgatgttatc ttggagatga tgtcagacaa gacaagcatg gaattggaag cggaagaatc
     6421 aggtgttctt ttgaaaatcg ttcacggtaa cggtgcaact gttcctgtaa cagaagtcat
     6481 tgcttacctt ggtgcagaag gcgaatcagt tgaagttggt agtgcacctg ctccagctga
     6541 ggttgctcaa gcaactgctg acttgaaagc agctggtttg gaagtacctg cagctcctgc
     6601 aacagctcca caagctccta aggctgaatt ggcagctgac gagtacgaca tggttgttgt
     6661 cggtggtggt cctgctggtt actacgcagc tattcgtggt gctcaattgg gcggtaaaat
     6721 cgctatcgtt gagaaatcag aatttggtgg tacttgcttg aacaagggtt gtatcccaac
     6781 taagacctac cttaaaaacg ctgaaattct tgacggcttg aagattgctg ctgagcgtgg
     6841 tatcaacctt gcttcaacaa actacaccgt tgatatggac aagacagttg atttcaagaa
     6901 caaggttgtg aagacattga ctggtggtgt tcaaggtctc ttgaaggcta acaaggtaac
     6961 aatcttcaac ggtcttggtc aagtaaaccc tgacaagact gttgtcattg gcgacaaggt
     7021 tatcaaaggt cgtagcatca tccttgcaac aggttctaaa gtgtcacgca tcaacatccc
     7081 aggtattgat tcgaagttgg tattgacttc tgatgatatc cttgacttgc gtgaaattcc
     7141 taagtcactt actgttatgg gtggtggcgt agtcggagta gaacttggtt tggtttacgc
     7201 atcatacgga acagaagtaa ccgttgttga aatggctgac cgtatcattc caggtatgga
     7261 tcgcgaagtg tctgttgaat tgcaaaaagt cctttctaag aaaggtatga aattcttgac
     7321 atctgttggt gtatctgaaa tcgtcgaagc caacaaccaa ttgacaatca agttgaacga
     7381 cggctcagaa atcgtttctg aaaaagccct tctttcaatc ggtcgtgtac cacaattggc
     7441 tggccttgaa aatcttaacc ttgaattgga tcgcggtcgt atcaaggtta acgaatacca
     7501 agaaacatca atcccaggta tttacgcacc aggtgatgtc aacggtacta agatgttggc
     7561 acacgctgct taccgtatgg gtgaagtggc agctgaaaat gccatccatg gtaaccacca
     7621 caaggctaaa ttggacttca caccagcagc ggtttacaca cacccagaaa tcgctatggt
     7681 tggtttgaca gaagaccagg ctatcgagaa atacggtaaa gaaaacatcc ttatcggtcg
     7741 caacagcttc actggtaatg gtcgtgccat tgcttctaac gaagctcacg gtttcgtaaa
     7801 agtaattgcg gacaagaaat actatgaaat ccttggggtt cacattatcg gtccagttgc
     7861 agctgaaatg atcaacgaag cagcaactat catggaatct gagttgacag ttgatgacgt
     7921 ggcagcatca atccacggtc acccaacctt ctcagaggtt atgtatgaag cattccttga
     7981 tgttcttggt gttgcaatcc acaacccacc aaaacggaaa taatataaag aatcaaaaga
     8041 aagtccacgt ggctttcttt ttttgatgtt cataggatag tatcaatctt tcgcagtttt
     8101 tgactgttat ttgatagaaa aacagactat acttgtagta gaaaaagggc aaggagaata
     8161 aagatgcgca agattatttt tttagatgtg gatggaacct tggttgacta tcataatcgg
     8221 attccagaat ctgccattcg tgccattcga caggccagag agaatgggca cttggtttat
     8281 gtctgtacag gtcgaagtcg ggcggaaatg caaccagaac tatgggagat tggtcttgac
     8341 ggaatgattg gtggcaacgg ctcttatgtc gagcatcaag gacagatggt catgcatcaa
     8401 gttatttcaa aagttgatag tcgagcgatt gtagactggc ttcatgaacg tggtttggaa
     8461 ttttatctgg aaagcaacaa cgggctcttt gccagtgaga atttccgtga acgtgcccgt
     8521 gaaaccctgc ggatatattc catgaacaaa gggaaaactg cagaagaggt tgccaatcaa
     8581 gaagtagaag atgtcataca cgggatggtg tttgatggag aattgtatcg agatgatttg
     8641 aataaagtca gttttgtact cgactcttac caggatcatt tggactccaa agaggccttt
     8701 ccgcaactgg ttgcaaacac ctggggaggc cgtggcgaga cagcactatt tggtgacttg
     8761 ggggtgaaag acatcgacaa ggctcatgct atttctgttt tgctggacca cttaggagct
     8821 agtcaggcag acactatcgc ttttggtgac gctaaaattg atatttccat gctggactat
     8881 tgtgcagttg gagtggctat gggcaatggc ggagcggaaa tcttggctat ggcagatatg
     8941 ataacggacg atgtagaaga agacggtctc tacaatgcct ttgaaagatt gggactactt
     9001 gacaagtagc tctcagaata aatcatagta tcccttgcaa aatcgcttac aatctgataa
     9061 gataagtgta ggattgttac tgataagcag gcaaaatcta agtagatagt gaagggtctg
     9121 gcaacagtcg tatttgctat gtgcttgggt tcttttttta tcagtcgaaa aaagaggcgg
     9181 taaagtagat gcagattgaa aaagtttata acaacaatgt cattcaagca atagatcagc
     9241 agggccgtga gttgattgtc atggggaaag gattaggttt tcagaaaaaa gcaggggaag
     9301 aattagacac cagcaaaatc gagaaaacct ttgtgctaca aaatgactac caacaatcag
     9361 atttatcaag tctgtacctg caaatggaga gcagtgaagt cgaagtagtc aatgccatta
     9421 tcaatagggc agaagaaact ttagaagttc agtttgattt gtctttgtat ctagccttag
     9481 cagaccattt acattttgta tttcagcgat gccgtgaagg tatctttatt gagaacccgc
     9541 tgtcttggga agtacgtaag ttttatccga aggaatacca aattggttta gaagccttgc
     9601 agttggtgca ggagcgttta ggcttggaac taggaaaggg agaggcctcg tctatcgctc
     9661 tccatctagt aaatgctcag aagaatggtg cttttggcaa ggaaacccaa accattagta
     9721 aaatcgtgac tcagatttta gacattgtcc gccttcattt tggtagtgtg acctacgagg
     9781 aggataccag ctatcatcga tttgtaacac atattcagta ttttgctcaa cgtgtggcca
     9841 atggtgtggt cgaaggggtc aatgatgcat ttttgtatga acaggtgatg gctaactatc
     9901 ctgattcatt tgcttgtacg actaagattc gtcagcatat tgcggaagtt taccattttg
     9961 aaatgtccaa agatgaacaa gtctatttga ccatccatat ccagcgctta aaaacgagtt
    10021 agaaaaaagt tagaaaaaaa tcaaaaaaac ttcagaaaac acttgcaatt taatttgcga
    10081 tgtgttatca taatctcaac aaaaggattg ttactggtta gcaggcaaaa cctaaatata
    10141 tacaagatac tctttttggc ttgggccaag aggtagtgtt tggtatatgt ttaggttttt
    10201 ctgtttttcc agaaaagaaa ggagccgaaa aatggctaag gattatacag atttagctac
    10261 agatattgta gcccacgtcg gaggaaaaga aaatattagc agtcttaagc actgtgtgac
    10321 tcgtcttcgt tttggtctca aggatgagag caaggctgat acagactact tgaaggcgcg
    10381 tgatggcgtt gtgacagtag ttcaagcagg tggtcaatac caagtggtta tcgggaacca
    10441 tgtgccaggc gtttatgcgg ctgtgcaaaa agttgcaggg atttcaggag atggcgcctt
    10501 ggatattgat gaaggtgatg gtccaaaagg aaatctcttt gaccagtttg ttgatttggt
    10561 ttcaggtgtt ttccagcctt ctttgggagt gcttgcagct gcaggtataa tcaaaggttt
    10621 ggtttctatc cttgcagcta caatgggatt gaataatgat aatagtgctc tttgtgcaat
    10681 tttaaacgca gcaggtgatg gtttcttcca attcttaccg attattttgg cctacaatgc
    10741 tgcacgcaaa ttcaaaatga atgaatttac aggtatggca attgctatgg ctttggttta
    10801 cccaactctg ccagggacat ttgcgactct aaaagaagcg ggtttaaata atgtttttgg
    10861 cctaccattt gtacttccag cagcaggtag ctacttgcaa actgtgatgc caattttact
    10921 tgcagtttgg gttgcttcac atattgagaa atttatgaag aaaatcacac ctgatgtggt
    10981 caaagtcttc attgttccat ttgcaacagt tttattatca gtgccattga cttttttagt
    11041 tgtaggccct attgcaaact atatttctga tttattgtct gctggattta ctgcagtgat
    11101 gggattcagt ccaatccttt atggactact tcttggggca ttatggcagg ttatggtcat
    11161 gtttggacta cactgggcac ttgtaccgct tgcgattctc caattctcac aaaatggctg
    11221 gtcaaatata ttgttggcag cagctcttcc aaactttact caaacaggag ttcttttggc
    11281 aattatgatg aaaaccaaag aatcaaaagt gaagtcagtt gcgatgcctg cttttatttc
    11341 ttctatattt ggtgtaactg agccagcaat ttatggcatc acgcttccaa tgaaaacacc
    11401 attctacatt tcttgtgcag tgtcagcagt aattggtgca ggtctctcat tctttgattt
    11461 gaaaaactat gctatgggtg ctctcggtat ctttatgtat ccagggtacg ttagtcctga
    11521 actaggtgtg actccaatgt ggattatgtt gggcttctct ttggtggcag ttgttttatc
    11581 cttcgctatc caaatggcgc ttccagttcc ttatttgtac ggagagccaa ctgaaaaaaa
    11641 gtagttgaga ctccacaaga atctatccct gagctaaaag aaatcaagca agagatcatt
    11701 gctagtcccc tcatcggtaa ggttgtcaaa ttggaagatg ttccagacgc agtctttgct
    11761 tcaggtgcta tggggaaagg aattgctatt gatcccctag atggtattct tgtttcacca
    11821 gctaaagctg aagtgaccct tgtttttcca accaagcacg ccattggtct tcggacggaa
    11881 aatggagcag agcttctcat ccatattggt atggacacag tctctctagc tggaaaaggt
    11941 tttgaatcat tcgtccaagt aggtgaccaa gttgaagcag gacaaaaatt gctggaattt
    12001 gatctccaac aaatcaaagc agctgaccta ccagtcatca ctccaattat cgtgaccaat
    12061 acagctgact atgaggacat cttggtaacc caagaaagcc aaattaacag tggtgattac
    12121 ctgttgacaa cggtcaaata aaaaataaag tggttggcag tgccaatcac tttattttgt
    12181 atcttgttta gcgttgaaga aaatccttat cctgctctgg aatgcccgcc tcatcactct
    12241 gatagcgttc agcggtgaca gtcagctggt acttacttcg caagtctagg atttcctgca
    12301 tcattggaga agcatggtgg atgtccaaag ctgcttggtc tatccagcta tcaaccaaga
    12361 ggattgaatg gggatcatcg agcggtgcaa agtattgata acgtgtattt ccaacctgct
    12421 tgcgaatgcg gtcggcaatg ccactagctt ccatttcctg agaaaaagca cgcgcattgt
    12481 cagctggtcc agtgtagtaa aggtgcatgg taatcatagt ataagatata aagggcgtta
    12541 agcggacaca gacaaaatat agtcttttag tttactttta gtactaggca atgagctgca
    12601 gacagtactg aagtacggca aagcgaatta acgaagtaat aaaagaaaaa ctaaatgacg
    12661 taggaaacag aacgacgatg caagcgtcga gttctcattt atctttttgg cacagtccgt
    12721 agcccgtact cagttcaacc aaatacggag gccctagtcc tttctattat ttatcaaaat
    12781 tagtataagt cctattcttc cgcagttgtt tgcggtagct gctaggtgtg gtatggtagt
    12841 attgcttgaa ctggcggttg aaattggata aattattaaa gccaacttcg gtcgcaattt
    12901 caagcacagg cttgagagtg tttgtcagga gttcggtagc tgcccgcagt cggacttgga
    12961 tgataaattc catacaggaa gttcctgttt gttgcttgaa gattgtcata aaatgggttt
    13021 ttgtatagcc catttgctct gccatttgct cgatggtgag gttttctgca taatgttgtt
    13081 ggatgtattc aatcaacaac cgcagttttt cattttttcg atacatatca tcggtatttt
    13141 ttcggacgat gtagcggtaa cggtagagca ggtagaagaa gtcaaagagt cggctcttga
    13201 gaggtagctc aaagtaaggt ggctggtgtc tgaccaactc aaaaatggtg agcaggcatt
    13261 gtctaatctc tgcgtagcca ggatcttggg gctggatacg tggaatacac ttgaaaacat
    13321 ctgtctgtaa gggttgtagg tagagcatac tggtctgatc taggtgagat gcccccagca
    13381 tatccaagtg aaagaggagg gtatccgttt catgtccccg ttgctcaatc ggatgaatgg
    13441 aatgcgcagc attgggtcga atcaggataa tatcaccagc ctgactattg aaataatcat
    13501 agtcgatatg gtaacgggct gtccctccgt aaacatggtt aatttcaaat tcattgtgcc
    13561 aatgaaagag gatgtcagga ataccatcta tgacactggt atggtaatac ttgtagggta
    13621 acaaacgact atcgtagtca atggtgtgct taaactgttt atccgttaat ggtggttgca
    13681 tacgttcctc cttttcgtag gatagtatca ttatagcata ttgtttgaga agaaaagggt
    13741 ttcatatctg tttatactat atttgttaga aaaaagataa gggagaagta agatgtcacg
    13801 atttccaaaa aatttcctct ggggaggagc tatcgcagcc aaccaggcag aaggcgccta
    13861 taatgtggat ggacgcggtt tagtccagac agatgttaca acaggaggat ctgtcaacag
    13921 cccccgctat acaacctaca ttgataagga cggcaagcca ggtaaatatg cttctatggg
    13981 gcatgctggc ggtcatctgc cagaaggggc gacctatgct gtcctagaag accactatta
    14041 tccaaaccac aaggctgtgg acttctacca ccgctataaa gaagatatca agctcttcgc
    14101 agaaatgggt tactccgttt tccgcttgtc gatttcatgg gcacggattt tcccaaatgg
    14161 tgatgatgca aagccaaacc aagcaggtct tgatttctat cgttctgttt ttgaagaatg
    14221 ccgcaaatat ggcattgagc cattggtttc catttggcac ttcgatacac ctttgagctt
    14281 ggagcaacgc tatggtggtt ggaccaatcg taagttgatt gatttctatg tccgctattc
    14341 tgagacaatt tttaaggaat ataaggggtt ggtgaaatat tggattacct tcaacgagat
    14401 taacaacact atcatgtttt tggatatgtt tagtgaacca aaggattctg attaccaaga
    14461 tgcctaccaa cacttacatc accagtttgt tgcctctgcc aaggctgtaa aattaggaca
    14521 tgagattgat ccgaactatc gattcggaaa catgatttgt ggaatcacct tctacacagg
    14581 aacctgtgac cctgcggata ttttagaaaa tcaacacaaa tgggagcaga atattttcta
    14641 ttgtagtgat gcgcaagcca aaggtcgcta tggttcctat gcaaacagat tgtggaagga
    14701 acacaatgtt gtattgcaaa tggaagaggg agacctagaa atccttgctg aaggcaaggt
    14761 ggatatgtat accttctctt attacatgtc aacagttgtg accactcata aagtggaaga
    14821 cacggtttct gggaacttct caatcggtgc caagaaccct tacttgacct attcagactg
    14881 gggttgggct caggatccga ccggtcttca atactatctt gagaaaatct acgaccgcta
    14941 tgaaattccg atgatgattg tagaaaatgg tttgggagct ttggatacag ttgaagaaga
    15001 tggctctatt cacgatgatt accgtatcga ctaccatcgt gcccatgtca atgcgatggc
    15061 agatgccatt gctaatggtg ttgatttgat tggctatacc acttggggct gtattgacct
    15121 agtatcagca ggaacaggtg aaatgcgaaa acgctacggc tttatctacg ttgatatgga
    15181 tgatgctgga aatgggacgc ttgctagaac accaaaagat tcattctact ggtacaagaa
    15241 agtcattgcc agcaatggtg aggatgtaga ataaggaaga cggcgatgtg ccgtttcaga
    15301 ttgaagaaaa agtccatttt ggacaatttt cttcaatctt tttttcttta tgttgaaaat
    15361 taaaaactct cagaaacgcc gaaatatcaa tgtttctaag agtttaatga cttgctatct
    15421 ttcgcaaact tcttcaattt tttatatttt taggagtttg tctatgttct gagacggtac
    15481 tttgccgttt tctcttaggg aaaggtttga aaatgaaaat agaacatgta gcaatctata
    15541 gtcaactgaa acaagagcag gatgaaactg cct
//
LOCUS       DATUCG010000052        15194 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.053, whole genome shotgun sequence.
ACCESSION   DATUCG010000052 DATUCG010000000
VERSION     DATUCG010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15194
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.053"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(108..1244)
                     /gene="dnaN"
                     /locus_tag="VCA20_001250"
     CDS             complement(108..1244)
                     /gene="dnaN"
                     /locus_tag="VCA20_001250"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEP1835075.1"
                     /translation="MIQFSINKNIFLQALSITKRAISTKNAIPILSTVKITVTSEGIT
                     LTGSNGQISIEHFISIQDENAGLLISSPGSILLEAGFFINVVSSMPDLVLDFNEIEQK
                     QIVLTSGKSEITLKGKEAEQYPRLQEVPTSKPLVLETKVLKQTINETAFAASTQESRP
                     ILTGVHFVLTENKNLKTVATDSHRMSQRKLVLDTSGDDFNVVIPSRSLREFTAVFTDD
                     IETVEVFFSNNQILFRSEHISFYTRLLEGTYPDTDRLIPTEFKTTAIFDTANLRHSME
                     RARLLSNATQNGTVKLEIANNVVSAHVNSPEVGRVNEELDTVEVSGEDLVISFNPTYL
                     IEALKATTSEQVKISFISSVRPFTLIPNNEGEDFIQLVTPVRTN"
     gene            complement(1398..2771)
                     /gene="dnaA"
                     /locus_tag="VCA20_001251"
     CDS             complement(1398..2771)
                     /gene="dnaA"
                     /locus_tag="VCA20_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEP1835076.1"
                     /translation="MNQEQLFWQRFIELAKVNFKPSIYDFYVADAKLLGINQQVANIF
                     LNRPFKKDFWEKNFEELMIAASFEIYGEPLAMQYQFTEDEQEIKNTTNARSSVVHQVQ
                     TSESATPQETFKPVHSDIKSQYTFANFVQGDNNHWAKAAALAVSDNLGELYNPLFIFG
                     GPGLGKTHILNAIGNKVLADNPQARIKYVSSETFINEFLEHLRLNDMESFKKTYRNLD
                     LLLIDDIQSLRNKATTQEEFFHTFNALHEKNKQIVLTSDRNPDHLDNLEERLVTRFKW
                     GLTSEITPPDFETRIAILRNKCENLPYNFTNETLSYLAGQFNSNVRDLEGALKDIHLI
                     ATMRQLSEISVEVAAEAIRSRKQTNPQNMVIPIEKIQTEVGNFYGVSLKELKGSKRVQ
                     HIVHARQVAMFLAREMTDNSLPKIGKEFGNRDHTTVMHAYNKIKTLLLDDENLEIEIT
                     SIKNKLR"
     gene            complement(2974..3738)
                     /locus_tag="VCA20_001252"
     CDS             complement(2974..3738)
                     /locus_tag="VCA20_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEP1835077.1"
                     /translation="MEELRTLNISEIHPNPYQPRIHFDEKELLELAQSIKENGLIQPI
                     IVRKSSIIGYELLAGERRLRASQLAGLTTIPAVVKELTDDDLLYQAIIENLQRSNLNP
                     IEEAASYQKLISRGLTHDEVAQIMGKSRPYISNLLRLLNLSSQTKQAVEEGKISQGHA
                     RQLVSFSEEKQAEWVQLILSKDLSVRTLEKLIAANKKKHTKLKQRDQFLKEQEDSLSK
                     TLGTATKIIKKKNGSGEIRISFNDLDEFERIINNFK"
     gene            complement(3799..4995)
                     /locus_tag="VCA20_001253"
     CDS             complement(3799..4995)
                     /locus_tag="VCA20_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEP1835078.1"
                     /translation="MERIPYMKKYLKFAILFVIGFFGGLIGALSASFFQPHVQQANSA
                     ITSVSNVQYNNETSTTKAVEKVQNAVVSVINYQKSANNSLGAIFGNIESSDELAVAGE
                     GSGVIYKKDGQYAYIVTNTHVINNAEKIDILLASGEKISGELVGSDTYSDIAVIKISA
                     DKVTAVAEFADSDTIKVGETAIAIGSPLGSVYANTVTQGIISSLSRTVTSQSKDGQTI
                     STNAIQTDTAINPGNSGGPLINTQGQVIGITSSKITSSSANSSGVAVEGLGFAIPAND
                     AVAIINQLEKTGQVSRPALGVHMVNLTTLSTSQLEKAGLSNTELTSGVVIVSTQSGLP
                     ADGKLETFDVITEIDGEAIQNKSDLQSALYKHQIGDTITVTYYRNNQKQTVDIKLTHS
                     TEELSE"
     gene            5169..5648
                     /gene="rlmH"
                     /locus_tag="VCA20_001254"
     CDS             5169..5648
                     /gene="rlmH"
                     /locus_tag="VCA20_001254"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEP1835079.1"
                     /translation="MKIKLITVGKLKEKYLKEGIAEYSKRLGRFTKLDMIELPDEKTP
                     DKASQAENEQILKKEADRIMSKIGERDFVIALAIEGKQFPSEEFSQKISDIAVNGYSD
                     ITFLIGGSLGIDSCIKKRANLLMSFGQLTLPHQLMKLVLIEQIYRAFMIQQGSPYHK"
     gene            5673..6356
                     /locus_tag="VCA20_001255"
     CDS             5673..6356
                     /locus_tag="VCA20_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEP1835080.1"
                     /translation="MNQKDYRVFEGLRIVCSLTFISGYLNAFTFVTQGGRFAGVQSGN
                     VISLAYFLAKGDFAQVVNFSIPILFFVFGQFFTYLARRYFEKQTWSWHFGSSVMMLVL
                     ILLTIILSPIMPASFTIASLAFVASIQVETFRRLRGAPYANVMMTGNVKNAAYLWFKG
                     VIEKDSELRKTGRNILLTIIGFMLGVIISTHLSFQFEEYALIGLILPVLYINYELWQE
                     KRPTRGRSK"
     gene            complement(6354..6427)
                     /locus_tag="VCA20_001256"
     tRNA            complement(6354..6427)
                     /locus_tag="VCA20_001256"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6391..6393),aa:Arg,seq:tct)
     gene            6539..6610
                     /locus_tag="VCA20_001257"
     tRNA            6539..6610
                     /locus_tag="VCA20_001257"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6572..6574,aa:Glu,seq:ttc)
     gene            6616..6688
                     /locus_tag="VCA20_001258"
     tRNA            6616..6688
                     /locus_tag="VCA20_001258"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6649..6651,aa:Asn,seq:gtt)
     gene            complement(6757..9336)
                     /locus_tag="VCA20_001259"
     CDS             complement(6757..9336)
                     /locus_tag="VCA20_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEP1835081.1"
                     /translation="MKKIFTKTSIYYLLSFLIPLTIISIVLAFQGIWWGSDTTILASD
                     GFHQYVIFNQTLRNTLHGDGSLFYTFSSGLGLNFYTLSSYYLGSFLSPIVFFFDLQSM
                     PDAIYLFTIVKFGLTGLSTYFSLKGIHKNLKEEWALLLATSFSLMSFSTSQLEINNWL
                     DVFILLPLVLLGLHRLLKKQGPILYYITLTCLFIQNYYFGYMVAIFLTLWTLVQLSWI
                     DSQRIKRFINFTIVSILSALSSMFILLPTYLDLKTHGETFTKIVNLKTEDSWYLDFFA
                     KNLVGSFDTTKFGSIPMISVGLVPLILALLFFTLKEIKPTVKLSYALFFTFIISSFYL
                     QPLNLFWQGMHAPNMFLYRYAWALSITVIYLAAETLVRLRQVSINNFTLIVSFLLICF
                     TSTFIFRDHYEFLTNVNFLLTLEFLIAYFILFVAMIRYKSSLKWINIVLLFFTFLELG
                     SHSHYQVQGISDEWHFPSRSNYEEKLTDIDSIVKSTKTTTDSFYRIERLLPQTGNDSM
                     KFNYNGISQFSSIRNRASSSVLDKLGFRSDGTNLNLRYQNNTIIADSLFGVKYNLATT
                     DPNKFGFTLNQSQSTINLYENSFNLGLALLTEGIYKDVNFTNLTLDNQKNFLNQLTGL
                     SQKYYHTLSDVVSQNTVELSNRMTVNKVVNEDAAKTTFLVNIPANSQVYLNLPNLTFS
                     NENPKKVVITVNNQSSEFTLDNAFSFFNVGSFTTDVQVQVNVYFPENNQVSFDKPQFY
                     RLDLLAFQQAISILQEKQVVTKTDGNKVTVDFVTDKESSLLLTLPYDKGWNATIDGKP
                     IKIQKAQDGFMKVDVSPGQTKLVLTFVPNGFYLGLLISFGTVFVFFSYKFIGYYYSKN
                     REY"
     gene            complement(9373..10995)
                     /locus_tag="VCA20_001260"
     CDS             complement(9373..10995)
                     /locus_tag="VCA20_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEP1835082.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTAGNTYGLIGANGAGKSTFL
                     KILAGDIEPSTGHISLGPDERLSVLRQNHFDYEDERVIDVVIMGNEQLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNISEDLHYQNMSELTNGE
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSINWLEDFLIDFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKIFVGNYDFWKQSSELAAKLQADRNAKAEEKIKELQEFVARFSANA
                     SKSKQATSRKKMLDKIELEEIIPSSRKYPFINFKSEREIGNDLLTVENLKVVIDGETI
                     LDNISFILRPGDKTALIGQNDIQTTALIRALMGDIEYEGTVKWGVTTSQSYLPKDNTR
                     DFDTNESILDWLRQFASKEEDDNTFLRGFLGRMLFSGDEVNKPVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKAFKEAIIFASHDHEFIQTLANHIIVI
                     SKNGVIDRIDETYDEFLENAEVQAKVQELWKA"
     gene            complement(11060..11932)
                     /locus_tag="VCA20_001261"
     CDS             complement(11060..11932)
                     /locus_tag="VCA20_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEP1835083.1"
                     /translation="MKERIKDFISVTLGSVVMAIGFNSFFLENNIVSGGVGGLAIALN
                     ALLGWSPSDLVLYCNIPLLIICWFFLGKSVFIKTVYGAIIYPLCIKLTAGLPNLTENP
                     LLAAIFGGIILGFGLGLVFLGNSSTGGTGILIQFIHKYTPLSLGLTMAIIDGIIVGLG
                     FVAFDTDTVMYSIIALMTITYIVNRMMSGTQSSRNVMIISQKSEEIKDYITKVADRGV
                     TELPIIGGFTGVDKRMLMTTISIPEMQKLETAVLEIDETAFMVVMPASQVRGRGFSLQ
                     KDHKHYDEDILIPM"
     misc_binding    12034..12240
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            12287..13312
                     /gene="trpS"
                     /locus_tag="VCA20_001262"
     CDS             12287..13312
                     /gene="trpS"
                     /locus_tag="VCA20_001262"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEP1835084.1"
                     /translation="MSKPIILTGDRPTGKLHIGHYVGSLKNRVLLQDGGQHELFVFLA
                     DQQALTDHAKEPQKIVESVGNVALDYLAAGLDPAKTTIFIQSQIPELAELTMYYMNLV
                     SVARLERNPTVKTEIAQKGFGEGIPAGFLVYPVSQAADITAFKANFVPVGNDQKPMIE
                     QTREIVRSFNHAYQTDILVEPEGIYPENEAAGRLPGLDGNAKMSKSLGNGIYLADDMD
                     TLKKKVMSMYTDPNHIRVEDPGKIEGNMVFHYLDVFGREEDKAEIEAMKEHYQRGGLG
                     DVKTKRYLLDILERELGPIRERRLEFAQDMGQVYAMLEAGSQKARQVAATTLDQVKSA
                     MGINYFK"
     gene            13544..15025
                     /gene="guaB"
                     /locus_tag="VCA20_001263"
     CDS             13544..15025
                     /gene="guaB"
                     /locus_tag="VCA20_001263"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003090086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEP1835085.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPHDIDLKTQLAPNLTL
                     NLPIISAAMDTVTDSKMAIAMARAGGLGVIHKNMSIAEQADEVRKVKRSENGVIIDPF
                     FLTPEHTIAEAEKLMATYRISGVPIVETLENRKLVGIITNRDMRFISDYSQPISTNMT
                     SDALVTAPVGTDLATAEAILHKHRIEKLPLVDENGRLSGLITIKDIEKVIEFPNAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIKEIREHFPTR
                     TLIAGNIATAEGARALYEAGVDVVKVGIGPGSICTTRVIAGVGVPQVTAIYDAAGVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGHAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKQGSKDRYFQASVNEANKLVPEGIEGRVAYKGSVADMIFQMVGGLRSGMGY
                     VGAGNLTELHENAQFIEMSGAGLKESHPHDVQITNEAPNYSVQ"
BASE COUNT         4729 a         2634 c         2983 g         4848 t
ORIGIN      
        1 atatatataa cgtcatttat tccccatcac taaatatata aaaataagct aggtgctatt
       61 ttttagtata acataagaac ggctagttta gccgttctta atattattta gttggtacga
      121 actggtgtaa ccaattgaat aaaatcttcc ccttcattat ttggaatcaa tgtaaatgga
      181 cggacagaag agataaagct aattttcact tgttcactag ttgtggcttt caatgcttct
      241 atcaagtaag ttgggttaaa gctgattact aagtcttcac ctgatacttc tacagtatct
      301 aattcctcat tcacacgtcc aacttctgga gaatttacat gagccgatac aacattatta
      361 gcaatttcta gttttactgt accattttgg gttgcatttg aaagaagacg agcacgctcc
      421 atcgagtgac gaagatttgc agtatcaaaa attgcagttg ttttaaactc agttggaatt
      481 aagcggtctg tatcagggta ggtaccttct aataagcgtg tatagaagct aatatgctcg
      541 cttctaaaaa ggatttgatt atttgaaaag aagacttcta ctgtttcaat atcatctgta
      601 aaaactgcag taaattcacg gagagaacga cttggaatga caacattaaa atcatcacca
      661 gaggtatcaa ggaccaattt ccgttggctc atacggtgtg aatctgttgc aacagttttt
      721 agatttttat tttctgttaa aacaaaatga acacccgtaa gaataggacg actttcttgt
      781 gtagaagctg caaatgctgt ttcattaatt gtttgtttta atactttggt ttctaacacc
      841 aatggttttg aagttggaac ttcctgtaaa cgaggatact gttctgcttc ttttcccttt
      901 aatgtgattt cagacttacc acttgtcaaa acgatttgct tttgttcaat ttcattgaag
      961 tcaaggacta aatccggcat actggatacg acattaataa agaaaccagc ttctaagaga
     1021 atggaacctg gagaactgat caaaagtcct gcattttcat cttgaataga aataaaatgt
     1081 tcaatcgaga tttgtccatt tgaaccagtt aaagtgattc cttcactagt tactgtaatt
     1141 tttactgttg aaagaattgg aatagcattt tttgtactga ttgcccgttt agtaatacta
     1201 agtgcttgta gaaatatatt tttattaata gaaaattgaa tcatggattc tcctttattt
     1261 attatctata aggttaatag taatagtagt ctctgtgtat tctgtggaaa agtgatgaaa
     1321 gatcaataaa actgcgggaa ttaacttgtt cacaacttgt ggaaaaaaca gagttttttt
     1381 aaaaagttat acacaggtta acgaagttta ttttttatac tggtaatctc tatttctaaa
     1441 ttctcatcat ccaagaggag agtttttatt ttattgtatg catgcataac ggttgtatgg
     1501 tctcgattac caaattcttt cccaattttt ggaagggaat tgtctgtcat ttcacgtgct
     1561 aaaaacatag caacttgtcg cgcgtgaacg atatgttgaa cacgcttaga accttttaat
     1621 tctttcaagc tgacaccgta gaaatttccc acttcggttt ggattttctc aataggaata
     1681 accatgtttt gtggatttgt ttgttttctt gatcgaatag cctcagcagc aacctcgaca
     1741 cttatctcag acagttgacg catagtggct atcaaatgga tatcttttaa ggcaccttca
     1801 aggtcacgta cgttcgaatt aaattgccca gctagatagg atagcgtctc atttgtaaag
     1861 ttgtaaggca ggttctcgca cttgttacgt aaaattgcga tacgtgtttc aaaatcaggt
     1921 ggagtgattt cactggttaa cccccatttg aaacgtgtta ctagtctttc ttccaaattg
     1981 tctaagtgat cgggattacg gtcgcttgtg agtacaatct gcttattttt ttcatgaagc
     2041 gcattaaaag tatggaaaaa ttcttcctgt gttgttgctt tattacggag agactgaatg
     2101 tcatcaatta gaagtaagtc cagattacga taggtttttt tgaaactttc catatcattg
     2161 agacggaggt gttctaaaaa ttcattgatg aatgtttccg atgagacata ttttattctt
     2221 gcctgaggat tatcggctag aaccttattt ccaatcgcat ttaaaatatg agtttttcca
     2281 agaccaggac caccaaaaat gaataatgga ttgtagagct cacctaggtt atcagataca
     2341 gctaaagctg cagcctttgc ccagtgatta ttgtctcctt gtacaaaatt agcaaaggtg
     2401 tactgggatt ttatatcaga atgaaccggt ttaaaagttt cttgaggagt agccgactca
     2461 gatgtctgta cctggtgaac cactgaactt cttgcgtttg tagtgttctt aatctcctgt
     2521 tcatcctctg taaattgata ttgcatggca agaggctctc cgtagatttc aaaactagcg
     2581 gcaatcatta actcttcgaa gtttttttcc cagaaatctt ttttaaatgg acgatttaag
     2641 aaaatattgg caacttgctg gttgattccg agtaattttg catcagcgac ataaaaatca
     2701 taaatagatg gcttaaaatt tacctttgcc aattcaataa atcgttgcca aaaaagttgt
     2761 tcttggttca tattttcccc ctttcacatt atattcgtta gaatagtgta ccataaaaaa
     2821 caaaagtttt ccacagataa ttttaaaaat ccacattgtt aagaaaagtt ttccacaaga
     2881 tgtgaataaa agtcagaacc tttgctggtc ctgacttttt gtaatgattt tagtgtggaa
     2941 aagagtgttt ataaaaataa attgtaaaca agtctattta aaattgttga taattctttc
     3001 gaattcatcg aggtcattaa agctaatccg aatttctccg ctcccgtttt tcttcttgat
     3061 aatttttgta gctgttccaa gagttttact gagtgaatct tcctgttctt ttaaaaattg
     3121 gtcgcgttgt ttaagcttag tgtgtttttt cttatttgca gctattaatt tttcaagcgt
     3181 acgcacactt aaatcctttg ataaaatgag ttgaacccat tcggcttgct tttcttctga
     3241 aaatgacacc aattgtcgcg cgtgcccttg tgaaattttt ccttcttcta cagcttgttt
     3301 agtctgagat gatagattta gtaggcgcaa taaattactg atatatggtc ttgattttcc
     3361 catgatttga gcaacttcat catgtgttaa ccctctacta atcaattttt gataagaggc
     3421 tgcttcttct atcggattta agttagaacg ctgcagattc tctatgatag cctgatagag
     3481 taaatcatca tcagtcagtt cctttaccac tgctggtatt gtagtcagtc cagctaattg
     3541 actggctcgc aaccttcttt ctccagctaa taattcgtat ccgataatag aagattttct
     3601 tacaataatc ggttgaatta atccattttc cttaatggat tgagcgagct caagtagctc
     3661 cttttcatca aaatgaattc ttggctgata gggattggga tggatttctg aaatatttag
     3721 tgtacgtaat tcttccatat atccttattc tagcaaaaaa aacgagactt tacaattgtg
     3781 taaagtctta tttgtcaatt attcgctaag ttcttctgta gaatgtgtca acttaatgtc
     3841 aacagtttgt ttctgattat tgcggtaata agttacagtg attgtatctc caatttgatg
     3901 tttgtagaga gcgctctgga ggtcactctt attttgaata gcttctccgt caatctcagt
     3961 aataacatca aaagtttcta attttccatc tgcaggtagc ccactttgtg tagagacaat
     4021 tactacaccg gatgttaatt ccgtatttga taatccagct ttttctaatt gactagttga
     4081 caaggtcgtc aagttaacca tatgaactcc aagagcaggt cggctaactt gtccagtttt
     4141 ttcaagctga ttgataatag ctacggcatc atttgcagga atagcaaatc ccaacccttc
     4201 tacagccacg cctgagctat ttgcagaact tgaggtaatt ttgctagagg taatgcctat
     4261 cacttgtcct tgggtattga ttaacggtcc gccagagttt ccagggttga tagctgtatc
     4321 agtttgaata gcgttagttg agattgtttg tccatctttt gattgtgaag taactgtccg
     4381 acttaagcta gaaataatac cctgggtaac tgtattggcg tagacgctac ctagaggact
     4441 accaattgcg atagcagttt ctccaacttt aattgtatcg gaatcagcaa attcagcaac
     4501 agcagtgact ttatctgctg atatttttat aacagctata tcagaatatg tatcggaacc
     4561 aacaagttca ccgctaattt tttctccaga tgctaaaagg atatcaatct tttctgcgtt
     4621 attaataaca tgcgtatttg tcacaatata ggcatattga ccatcttttt tatagataac
     4681 cccagacccc tctccagcaa cagctagttc gtcagatgat tcaatatttc caaagatagc
     4741 accaagacta ttgttggctg atttttggta attaataaca gacacaacag cattttgtac
     4801 tttctctaca gcttttgtgg tggaagtttc attattatat tgaacattgc tgacactagt
     4861 gatagcagaa tttgcttgtt gcacgtgtgg ctggaagaaa gaagctgata aggccccgat
     4921 aagaccccca aaaaatccaa ttacaaataa aatcgcaaat ttcaaatatt ttttcatata
     4981 aggaatcctt tccatttctt taattttctt taagagtact atttctttct taattataac
     5041 ttaaagtttt caaaaatcaa tccacaacct gtggataact ctgcgaaata tgtgaaaaac
     5101 tgtagccata tcctgatttt ctaaaaatat tttctgtcgt atgcttgtgg atatgttaca
     5161 attattttat gaaaataaaa ttgattaccg ttggaaaatt gaaagaaaag tacctcaaag
     5221 aaggtattgc agaatatagt aaacgattgg gacgatttac taagttggat atgattgagc
     5281 ttcctgatga aaaaacacca gataaagcca gtcaggcaga gaatgaacaa atattaaaaa
     5341 aagaagccga tagaattatg tctaaaattg gagagcgaga ttttgtcatt gccttagcga
     5401 tagaagggaa acaatttcca tcggaagaat ttagtcaaaa gatatctgac attgcagtaa
     5461 atgggtattc agatataact tttctcatcg gtggtagttt gggtattgat tcttgtatta
     5521 aaaaaagagc taatttgttg atgagttttg gacagttgac acttccccat caattaatga
     5581 aattagttct catcgagcag atttatcgtg catttatgat tcagcaggga agcccatatc
     5641 ataagtagtt tcacgtgaaa caaggagtga aaatgaatca aaaagattat cgtgtttttg
     5701 agggattgag aattgtttgt tcattaacgt ttatcagtgg ttatttaaat gcctttactt
     5761 ttgtgactca gggtggtcgc tttgctggcg tacaatctgg aaatgttatt tccctagctt
     5821 attttttagc taaaggtgac tttgctcagg tagttaattt ttccattccc attttatttt
     5881 ttgtattcgg acaatttttt acctacttag caagaaggta ttttgaaaaa caaacatggt
     5941 cttggcactt tggtagtagt gtaatgatgt tagttcttat tttactaact atcattctct
     6001 cacctataat gcctgcgtct tttacaattg ctagtctagc cttcgtagcc tctattcaag
     6061 tagaaacatt tagaaggtta cgaggtgctc cgtatgccaa tgtgatgatg acagggaatg
     6121 tcaaaaatgc tgcttatctc tggtttaaag gagttattga aaaagattca gaacttagaa
     6181 aaacaggtag aaacatctta ttgactatta tagggtttat gctaggtgtc atcatatcta
     6241 ctcacctatc cttccaattt gaagaatatg cccttatcgg tctgattttg ccagttttat
     6301 atattaatta tgaattatgg caagaaaaaa gacctactcg aggtaggtct aaatgattcc
     6361 agcaggattc gaacctgcga ccgttcgctt agaaggcgaa tgctctatcc agctgagcta
     6421 tggaacctca actattttat tttacattaa ataaagcact ctgtcaatac aataaaaaat
     6481 ccaaaaatgt attgacaaaa aaagtcacat gtattatact agttattgtt ctaaaaatgg
     6541 tccgttggtc aaggggttaa gacaccgcct tttcacggcg gtaacacggg ttcgaatccc
     6601 gtacggacta tttcttccgc catagctcag ttggtagagc gcatgactgt taatcatgat
     6661 gtcacaggtt cgagccctgt tggcggagta aataaacact aggaggcctt gagaaatcaa
     6721 ggttttttgt tttttacaag aaaaaaccaa gaaaatttag tattctcggt tcttagaata
     6781 atagtatcca atgaatttat aggagaaaaa tacaaaaact gtaccaaaag aaatcagtaa
     6841 acctagatag aaaccatttg gtacaaaggt taaaactagt ttagtttgac ctggacttac
     6901 atccactttc ataaaaccat cttgcgcttt ttggattttg ataggtttac catcaatggt
     6961 tgcattccat cctttatcat agggtaaagt aaggaggagt gaggattctt tatcggttac
     7021 aaaatcaacg gttactttat taccatcagt ttttgttact acttgttttt cttggagaat
     7081 ggaaatagcc tgttggaaag ctaataagtc caatcgataa aattgtggtt tatcaaaaga
     7141 aacttgatta ttttcaggaa aatatacatt gacttgaacc tgtacatctg tagtaaagct
     7201 ccccacattg aagaaagaga aagcattatc tagtgtaaac tcacttgact gattgttgac
     7261 agtaatgaca actttttttg gattctcatt tgagaatgtt aaatttggca aatttaagta
     7321 tacttggcta tttgcaggta tatttactaa gaaagttgtc tttgcagcat cctcattgac
     7381 aactttgttg acagtcattc gattgctcag ttcaactgta ttttgtgaaa caacatctga
     7441 taaagtgtgg tagtattttt gagatagccc tgttagttga ttaagaaaat ttttttgatt
     7501 atcgagagtc agatttgtaa aattgacatc tttgtaaatc ccttcagtca atagggctaa
     7561 ccctagatta aaactatttt cgtaaagatt aatagtagac tgactctgat tcagtgtgaa
     7621 accaaactta tttgggtccg tagtagctaa attatatttg actccgaata ggctatcagc
     7681 aataattgta ttattctgat aacgaagatt caaattggta ccgtctgaac ggaaaccgag
     7741 tttatctagt actgagctgg aagcacgatt tcgaatggag gagaattgag aaataccgtt
     7801 gtaattgaat ttcatgctat cattgccagt ttgtggtaat agacgttcta tacgataaaa
     7861 tgaatcagtt gtagtctttg tagactttac aatactgtca atatctgtca atttttcttc
     7921 atagtttgat cggctaggaa aatgccattc atcagaaatc ccctgaacct gataatgact
     7981 atgtgatcca agttctaaga aagtgaagaa caataaaaca atattaatcc attttaagga
     8041 tgatttatag cgaatcattg ctacaaaaag aatgaagtag gctattagaa attctagtgt
     8101 gagtaggaaa tttacattcg ttagaaactc ataatgatct ctaaaaataa aggtagaagt
     8161 aaagcaaatg agtaaaaatg aaacaatcag agtaaaattg ttaatactta cttgacgtag
     8221 tcgtaccaat gtctctgctg ctaaatagat aacggttatt gatagagccc atgcataacg
     8281 atagaggaac atgtttggcg catgcatacc ttgccaaaaa agattgagtg gttgtaggta
     8341 gaaacttgaa ataatgaatg taaagaatag agcataggag agtttgacag ttggttttat
     8401 ttcttttaat gtaaagaata gtaaagcgag tattaatgga acgagaccaa cagagatcat
     8461 gggaatagaa ccgaattttg ttgtatcaaa gctaccaact aagttttttg caaagaagtc
     8521 cagataccaa gagtcttcgg tttttaggtt gacaattttt gtaaatgtct caccatgagt
     8581 ttttaaatct aagtaggttg gtaacaatat gaacatgctg cttagagcag ataaaattga
     8641 cactattgta aagttgataa atcttttgat tctctgacta tctatccatg ataattgaac
     8701 caatgtccat aatgttagaa aaatagccac catataaccg aagtaatagt tttggataaa
     8761 caagcatgtt aatgttatgt agtagagaat cggaccctgt ttttttaata atcgatgcaa
     8821 tccaagaaga acgagtggga ggagtataaa aacgtctagc cagttgttta tttctaattg
     8881 acttgtacta aaactcatca gagaaaaact agtagcgagt aatagtgccc attcttcttt
     8941 caaattttta tggataccct ttaggctgaa ataagttgac aacccagtca atccaaattt
     9001 gacaatagtg aaaaggtaga tagcgtcagg catggattgt aaatcaaaga aaaagacaat
     9061 tggagatagg aatgagccta ggtaatagga ggatagggta taaaaattga gtcctaaacc
     9121 acttgaaaat gtgtaaaata aagagccgtc tccgtgtaaa gtatttcgta atgtttgatt
     9181 aaaaataaca tactgatgga agccatcact ggccaatatt gttgtatcac ttccccacca
     9241 gattccttga aatgctaaga cgatagaaat aatagtcagc ggaataagga aagataagag
     9301 ataataaatg gatgtttttg taaatatttt tttcatagat ttaaaagaaa aggcgaataa
     9361 atcgcctttt tattatgctt tccaaagttc ttgtactttt gcttgtactt cagcattttc
     9421 caagaattca tcataagttt cgtcgattcg gtcgataaca ccgtttttag aaatgacgat
     9481 gatatggttt gctaaagttt gaataaattc gtggtcatgg ctggcaaaga tgatggcctc
     9541 tttaaaagct ttcaaaccat cgttcagact ggaaattgat tccaagtcca agtggttggt
     9601 tggatcatct aagaccaaga catttgactt gaggagcatg agttttgaca acatcacgcg
     9661 cactttttcg ccccctgaca agacgtttac aggtttgtta acctcatcac ccgagaagag
     9721 catacgtccc aagaaaccgc gcaagaaggt attgtcatct tcttccttgc tggcaaattg
     9781 acggagccaa tcaaggatag attcgtttgt atcaaagtca cgagtattgt cttttggtaa
     9841 gtaggattga ctagtagtga caccccactt gacagtacct tcatattcaa tatcgcccat
     9901 aagagcacga atgagagcag ttgtttggat gtcgttttga ccaataagag cagtcttgtc
     9961 acctgggcgc aggataaagc tgatattgtc aagaatcgtt tcaccatcaa taacaacttt
    10021 caagttttca actgttaaga ggtcattacc gatttcacgt tctgatttga agttgataaa
    10081 tggatactta cggctagaag gaatgatttc ttccaactcg attttgtcca acattttctt
    10141 acgtgaagta gcttgcttag acttagaagc attagcagag aaacgggcaa cgaattcttg
    10201 tagttctttg attttttctt ctgcttttgc attacggtca gcttgtagtt tagctgctag
    10261 ttcgctagat tgtttccaga agtcatagtt accaacgaaa atcttgattt taccgaagtc
    10321 aaggtcggcc atatgggtac atactttgtt taagaagtgg cggtcgtgtg acacgacgat
    10381 aaccgtattt tcaaagtcaa tcaagaaatc ttcgagccag ttgatagatt ggatgtccag
    10441 accgttggtt ggctcgtcca aaagaagaac atcaggctta ccaaagaggg ccttggccaa
    10501 gaggacttta accttctcac cgttggtcaa ttcgctcatg ttttggtagt ggaggtcttc
    10561 tgaaatgttg aggttttgga gcaattgaga agcttcactt tcagcttccc agccaccaag
    10621 ttcagcaaac tcaccttcta attcagcagc acgaacacca tcttcatcag aaaaatcttc
    10681 cttcatgtaa atagcatctt tttctttcat gatgctgtaa agttgctcat ttcccatgat
    10741 aacaacgtca atcacgcgct catcttcata gtcgaaatgg ttctgacgta gtacagaaag
    10801 gcgttcgtct ggtccaagtg aaatatgacc tgttgatggc tcgatgtcac cagcaagaat
    10861 tttcaaaaat gtagatttac cagcaccatt ggcaccaatc aaaccgtagg tatttcctgc
    10921 tgtaaatttg atattgacat cgtcaaataa tttgcggtcg ctaaatcgaa gcgacacgtc
    10981 tgatactgta agcatagttt ctcctctatt tctgtctatt agttttattg tactagaaag
    11041 aacttgaatt ttcaatgaat tacattggaa tcaaaatatc ttcatcataa tgtttatggt
    11101 ctttttgaag actgaagcca cgtccacgca cctgactcgc aggcataaca accataaagg
    11161 cagtttcatc aatttctaat accgctgtct ccagtttctg catttctgga atagagatgg
    11221 tagtcatcaa catgcgcttg tcaacccctg taaagccacc gataattgga agttctgtta
    11281 caccgcgatc agcaacttta gtaatatagt ctttaatttc ttctgatttt tgagaaataa
    11341 tcatgacatt tcgagaagat tgggtaccag acatcatgcg gttgacaata taagtaatgg
    11401 tcattagggc aataatagag tacataacag tatcagtatc gaaggcaaca aatccaagac
    11461 caacgataat accatcaata atagccattg tcagacctaa tgataaaggg gtgtacttgt
    11521 gaataaattg aataagaata cctgttccac cagtagaaga atttccaagg aagacaagcc
    11581 caagtccaaa acctaggata attccaccga aaatagcagc taggagagga ttttctgtca
    11641 agttgggtaa acctgctgtc aacttgatgc aaagaggata gatgatagct ccatagacag
    11701 ttttgataaa tacagatttt cctaagaaaa accaacagat gattaataaa gggatgttac
    11761 agtagaggac aaggtcagac ggactccacc ccagaagagc attgagtgcg attgctaaac
    11821 ctccgacacc accagatacg atattattct ctagaaaaaa actgttaaat ccaatggcca
    11881 tgacaactga tccgagtgtg acagagataa agtcctttat acgttctttc atatattctc
    11941 cttggttagt gttataaaag caatttctat tatccctaat ttagacagta tcgtcaaggc
    12001 tttgtcagct tgactttcta attttttgca gttataatag gaatcaatca gaagagtagt
    12061 aagtggataa tttttagaga gtttgtggtt ggtgtaaaca aacaagagtc gcttatgaat
    12121 ggactgagtg aatagaatct gaataaataa ggattcggct agcaggcctt atgtgcaaaa
    12181 agatataagc ttatttaatg cttataagtg aggtggcacc gtgtccttga cgccctcgca
    12241 cagttttctg tgcgtgggct tttattatgt aaaaggagaa tattttatga gtaaaccgat
    12301 tatcctaaca ggagaccgtc caacaggcaa attacatatt ggacattatg ttggttcgtt
    12361 gaaaaatcgt gttttattac aggatggagg tcaacatgaa ctttttgtct ttttagcaga
    12421 ccaacaagcg ttgacagatc atgcaaagga accgcaaaag attgttgaat ctgttggaaa
    12481 cgtagcacta gattatctag cggcaggact tgatccagct aagacaacga ttttcatcca
    12541 gagtcaaatt cctgaattgg cagagttgac catgtactac atgaacttgg tatctgttgc
    12601 acgtctagaa cgcaatccta cagtgaagac tgagattgct cagaagggat ttggcgaagg
    12661 aattccagct ggtttcttgg tctatcctgt gtctcaagca gctgacatca cagccttcaa
    12721 ggcaaatttt gtccctgttg gaaatgacca gaaacctatg attgaacaga ctcgtgaaat
    12781 cgttcgtagc tttaaccatg cttaccagac tgatattttg gtagagccgg agggcattta
    12841 tcctgaaaat gaggcagctg gtcgcttgcc aggtttggac ggcaatgcca agatgtctaa
    12901 gtcacttgga aatggtattt atctggcaga tgacatggat acgctcaaga agaaagtcat
    12961 gtccatgtac actgatccca accacatccg tgttgaagac ccaggtaaga ttgaaggaaa
    13021 catggttttc cactatcttg acgtttttgg tcgcgaagaa gataaggcag agattgaggc
    13081 tatgaaggag cactatcaac gtggtggttt gggtgatgtg aagaccaaac gttatttgct
    13141 ggacatttta gagagagaat taggaccaat ccgtgaacgc cgtctggagt ttgcacagga
    13201 tatgggacag gtctatgcaa tgttggaagc aggtagccaa aaagctcgcc aagttgctgc
    13261 gacaactctt gaccaagtga aatctgctat gggaattaac tattttaaat aaggttaaca
    13321 atgaaagagc aatgttgaga aaatgttgct tttttcattt gttttacgac gtagcaaaaa
    13381 acaaacgttc gaattttaca tataaaactg aatagaattt tctgaatttt taggaataat
    13441 gatattaaag cgaataatga ttgacaaatg atttcaaaat gttatcataa ttaaaataca
    13501 aatagcaatc atgctacata ttaaataaga aaagaggaag aaaatgtcaa actgggacac
    13561 taaatttttg aaaaaaggct ttactttcga cgatgtatta cttattccgg ctgaaagtca
    13621 tgtattacca catgatattg atttaaaaac acaattagca ccaaatttga cccttaatct
    13681 tccgattatt tctgctgcca tggatacagt aacagatagc aaaatggcta tcgctatggc
    13741 gcgtgcaggt ggtttgggcg tcatccataa gaacatgtct atcgcggagc aggctgatga
    13801 ggtgcgtaag gtaaaacgtt ctgaaaatgg agtcattatc gatccattct tcttaacacc
    13861 agaacacacc attgctgaag ctgagaaatt gatggctact taccgtattt ctggtgtgcc
    13921 aatcgttgaa acattggaaa atcgtaaatt ggttggtatc atcaccaacc gtgatatgcg
    13981 tttcatttcc gactattcac agccaatttc gaccaacatg acaagcgatg ctttggtaac
    14041 agcgccagtt ggaacagatc ttgcgactgc ggaggctatt cttcacaagc atcgtattga
    14101 aaaattgcca ttggttgatg aaaatggtcg tttgtcaggc ttgattacca tcaaagatat
    14161 tgagaaagtg attgagttcc caaatgctgc gaaggatgaa tttggtcgtc tcttggttgc
    14221 gggtgctgtt ggtgttacat ctgatacttt tgaacgtgca gaagcccttt ttgaagcggg
    14281 tgcagacgcg attgtcatcg atacagctca cggtcactca gcaggcgttc ttcgcaagat
    14341 taaagaaatt cgtgaacact tcccaactcg taccttgatt gccggcaaca ttgccacagc
    14401 tgagggagct cgtgctcttt atgaagctgg tgttgatgtt gtgaaggtcg gtattggacc
    14461 aggttcaatc tgtacaacac gtgtcatcgc aggtgttggg gtaccacaag tgactgctat
    14521 ctatgatgca gctggtgttg cgcgtgaata tggtaaaacc attatcgctg acggtggtat
    14581 caagtattca ggagacattg tgaaggcact tgcagcaggt ggtcatgccg ttatgctggg
    14641 ttctatgttt gcaggaacgg atgaagcacc aggtgaaaca gaaatcttcc aaggtcgtaa
    14701 atttaagact taccgtggta tgggctctat cgctgccatg aaacaaggtt cgaaagaccg
    14761 ttacttccaa gcatctgtaa atgaagctaa caaacttgtt ccagaaggaa ttgagggacg
    14821 tgtagcctat aaaggatctg ttgcagatat gatattccaa atggttggtg gacttcgctc
    14881 aggtatgggc tatgtaggtg ctggcaactt gaccgaattg catgaaaatg ctcaattcat
    14941 cgaaatgtca ggtgctggtt tgaaagaaag tcatccacac gatgttcaaa tcacaaacga
    15001 agctccaaac tattcagttc agtaaaacaa aaacaagacc ctgttgacag gcaatgtcat
    15061 taagttaact aacctatcac attttgtggt gggttatttt tatattacac taaaaataaa
    15121 aggggaaaag tcatgctaga tcagattaaa gctcatttac ttgatagtat taacgacatc
    15181 gtttccatcg caaa
//
LOCUS       DATUCG010000053        14892 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.054, whole genome shotgun sequence.
ACCESSION   DATUCG010000053 DATUCG010000000
VERSION     DATUCG010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14892
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.054"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(425..748)
                     /locus_tag="VCA20_001264"
                     /pseudo
     CDS             complement(425..748)
                     /locus_tag="VCA20_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018364639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
     gene            complement(759..3491)
                     /locus_tag="VCA20_001265"
     CDS             complement(759..3491)
                     /locus_tag="VCA20_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEP1835086.1"
                     /translation="MKDRKIILIDGGDKSENIESLQSIRYKGKLCFEIYFKNNVQPYR
                     YNVQRVEVLKFSKQLNPSKIGVYRRQDGVLLNNIESIFEFNGTHSRAYIIRYKNGTGK
                     LYMGRDLEIRQNELTHSNSRHVFSYLTELSKLNPLRNSGTDQLLLLKRYEELDYVDKT
                     VALASYLSPSKKNDLPLHGELIFPFGCNNSQYKATKNALINQFSVIQGPPGTGKTQTI
                     LNIIANIVIRNKTVLIVSNNNAAIDNIYEKLKNYGLDFPVAYLGSEKNKNNFILNQYS
                     MYPDFSSWRLKDNGMESLDKELNEKIRRLPRIFEIQEELANLRQELSELETEFRHFKD
                     FFEHIDISPIELNLYSSKKLFKLWNEFEEFYKKNKRLGLIFKIKNFLLYGVKNWELYK
                     QELSDLTIQFQNLFYQHKQTEIRNKLETLQEELVHLNGTEVLNNLTSDSLKIFKDFLV
                     SKYGNGRNRVQFTKEDLREQSKRVLLEYPIVLSTTFSSTTSLGKNITYDYLIIDEASQ
                     VDITTGALALSCAKNVVVVGDSKQLPHIVTKDVKESSAQLFHKYGVHSGYEYTNSFLQ
                     SVLKVIPNIPETLLREHYRCHPKIINFCNQKFYNGDLVIMTGDNGEKDVLQAIRTPKG
                     NHARDHYSQREIDIILKDVIPNYEIYRERTGVVTPYRKQKEKLQEQIDDLESDTVHKF
                     QGKEKDTIIISTVDNSITRFTDDPYLLNVAISRAKNKLFLVMTGNEQSLDKNISDLLG
                     YIQYNNFEVRESPVYSIFDYLYKQYEQEKEEYFRKRKRISRYDSENLMYHRIREVFMS
                     DEKYNSLDVIHDYKLHNLVKDFTRLTEVEAKYARHGNTHLDFLIINRLTKAPVLAVEV
                     DGYAFHKGNSKQFERDIMKDNILKKCDIPLNRFKTNESQEKEKLIEILDGLLGR"
     gene            complement(3517..4776)
                     /locus_tag="VCA20_001266"
     CDS             complement(3517..4776)
                     /locus_tag="VCA20_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HEP1835087.1"
                     /translation="MHQITCPHCGTAFQVNETEYSQLLAQVRGAEFDKEIHDRLERER
                     ELLSQKAENDLQARLSDKDKEILELTAKLDKFASQTELEISSAISKKDQEIQELKAQL
                     GQIGLAKDLELQQAVAQVEKERDAAQNALAMQEQKQELALATTRQEYEVRLKAADEQV
                     EFYKNFKAQQSTKAIGESLEQFAEAEFNKVRSYAFPRAKFAKDNEVSASGSKGDFIFR
                     DFDESGLEFISIMFEMKNEADTTKTKHKNADFFKELDKDRREKKCEYAVLVSMLEADN
                     DYYNTGIVDVSHEYEKMYVIRPQFFIQLIGILRNAALNSLQYQQELALVKEQNIDITH
                     FEEDLEIFKNAFAKNYQSASNNFQKAIDEIDKAIKRMEAVKAALTTSENQLRLANNKL
                     DDVSVKKLIRNNPTMKAKFEALREEED"
     gene            complement(4783..5040)
                     /locus_tag="VCA20_001267"
     CDS             complement(4783..5040)
                     /locus_tag="VCA20_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835088.1"
                     /translation="MKKWWEQLKEEYYETRFDSNEDWMEWFIKRKMTWAWRWPTAITI
                     FVLWALSLPDARNAVRFFYFLLALGVATVLHKLISYLKRRK"
     gene            complement(5058..5528)
                     /locus_tag="VCA20_001268"
                     /pseudo
     CDS             complement(5058..5528)
                     /locus_tag="VCA20_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937661.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            5923..6738
                     /locus_tag="VCA20_001269"
     CDS             5923..6738
                     /locus_tag="VCA20_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEP1835089.1"
                     /translation="MIKLIALDMDGTLLNEKKELMQPQINAIHQAVEAGVKIVLCTGR
                     PLAGVKPFVEQLGFDTEEEFIIVNNGCSTHSTKDWVLIDWEELSISDIDYLSTFIEND
                     HVQISLFDENDYFVLAEKANDRVNLDASLVGMIPQPIDLDEATSGKHRFFEAMFVGEK
                     VHIDAFENQHNTVLSKRYSTVRSQDYLLEILPNGASKATGLKKLADRLGILPEEIMAI
                     GDANNDLEMIEFAGLGIAMGNANVQVKAIAQDITDTNENNGVAKAIEKHILNK"
     gene            6748..7968
                     /gene="pepT"
                     /locus_tag="VCA20_001270"
     CDS             6748..7968
                     /gene="pepT"
                     /locus_tag="VCA20_001270"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEP1835090.1"
                     /translation="MKYPTLLDRFLVYVKENTRSDENSTTTPSTQNQVEFAQNILLPE
                     MERIGLQNVHYLPNGFAVGTLPANDPSLTRKIGFIAHMDTADFNAEGVKPQIIENYDG
                     NPIALGTSGYELHPKDFPQLANYHGQTLITTDGTTLLGSDDKSGIAEIMTAIEYLVQN
                     PDIKHCEIRVGFGPDEEIGAGADKFDVEDFDVDFAYTMDGGPLGELQYETFSAASAKI
                     DFLGRNVHPGSAKDQMINAFQMAIDFHNALPEADRPEKTEGYEGFFHLMNMEGSVDTA
                     STTYIIRDFEEEDFQARKQLMLDIAEKMNAKFDTPRVIVNLHDQYYNMKKIIEKDMTP
                     IKIAKDVMENLGIKPLIEPVRGGTDGSKISFMGIPTPNIFAGGENMHGRFEFVSLETM
                     EKAVDVILGIVAYK"
     gene            8095..8493
                     /locus_tag="VCA20_001271"
     CDS             8095..8493
                     /locus_tag="VCA20_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835091.1"
                     /translation="MTEETLAQGILIGIWGTTLLFSFIWYILVAISNYILFKKAGYAG
                     WKSLIPIYNLYIQQCIAFGYEKRWFILFLLIPLAGPLYGIYLVYNFGRSFGLSAVQAI
                     FYVLLTPIFNLYIAFNDGSRYQGPQEFFID"
     gene            complement(8654..9568)
                     /locus_tag="VCA20_001272"
     CDS             complement(8654..9568)
                     /locus_tag="VCA20_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEP1835092.1"
                     /translation="MNELELLARALGFIFVIGLGYTLKVKGIVRREDARIFSSLVMNI
                     TLPASLLVASATAQVSIDLYVPFILGILCNLFLDMVGYWEAKKSGQQTSVGLMQLSGY
                     NIGTFTLPFVQAFFPLSSLLSVIVFDAGNAIMVLGGNYSIAAGVDEEQERMTVATFIK
                     NLSRSVPLVVYLVSLSLAVCSIKLPRELISALTIASNANPFLAMLMLGILLDLKLNWK
                     EISRLVYLLSLRLGANILIAGVIYFLLPIDKTMKIMLLVCLVSPISVMSPIYALKLGS
                     RSAEPANVNSLSILISLVMMVLLIFLFV"
     gene            9699..10016
                     /locus_tag="VCA20_001273"
     CDS             9699..10016
                     /locus_tag="VCA20_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEP1835093.1"
                     /translation="MYYPVSSILIEFLVLAIVEKQDSYGYEISQTIKIVADIKESTLY
                     PILKKLEKAGYVTTYTQEFQGRKRKYYTITSNGREHLPFLQGEWNSYKDHIDGIIEGS
                     LRK"
     gene            10013..11125
                     /locus_tag="VCA20_001274"
     CDS             10013..11125
                     /locus_tag="VCA20_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HEP1835094.1"
                     /translation="MTRADYLNQLEQALFQLHPSARQEALDYFNEYFDEKDNDEEAII
                     ELGTPDDAAKEIIANLPEDALITEKDQASPHSSKPFDFNFDFDFQFDWENFFNNFKHS
                     AYQARERIKLTSKIEELPEFSNLQISLEDQALSVQSYDGETVLLHNPVSEDGSYQAFD
                     YQLVQDSLYISNKPLPDQLYFSNNQTSSAILKIPKRLLPLTSLNLKTTDSSLTIVDVQ
                     VCEQMVVNAKDSSISMTKVSCPSSALRLEDSTLTISDGQMSELKLEASDAVITLSSMS
                     LSDARITLEDCVWKLKDFVLEKSLNCQAEDSVLTYKPSTDISLDIWEEDSSLKLPKDK
                     AFTMMKEDDITHLSYKQENSPAQLVIHCQDCQLTVG"
     gene            11137..11715
                     /locus_tag="VCA20_001275"
     CDS             11137..11715
                     /locus_tag="VCA20_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEP1835095.1"
                     /translation="MNRKEYLQKLEKHLKHLPKEDFQDTLDYFNEFFDEKENDEEAIQ
                     VLGSPKEAAREILANLYDKEKIEDKPNTRNMVWLTILAILAAPIGIPLAISLVALFII
                     LLLIIFSLFLLLISLWIVLLSLAIAQLLLAFDLFTLSWSTSLLFTGLALICLAFTLLG
                     SKFTLDLGSKTFLHVLHWIQRKIRKGGYYETA"
     gene            11702..12556
                     /locus_tag="VCA20_001276"
     CDS             11702..12556
                     /locus_tag="VCA20_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HEP1835096.1"
                     /translation="MKQLKPFTKISLSLLGLGAILSLIGYLAGGWTNMKTVAENYFQP
                     QTASFTDIRSIEVNEKLSIMPSPDNQFHLHYYRNQHKDIDLLSHQVQDGKLTISSAYQ
                     GIVTYDGLLEPILNLVNDQNVAYYQPVLQVPKNSTIETLSGNLQGDVYLDKVTIQDFQ
                     LFVENGDMLIENSHISLSRLLHINGEVAINQSILTSPADITDTTGSILTVEDGDFKAE
                     NLKLEGRHGISNYNGSIDISLHSKTKTNIHIDASQNNLGIDLPTYSNPQATNYLYIST
                     LDGELNIQ"
     gene            complement(12593..12895)
                     /locus_tag="VCA20_001277"
     CDS             complement(12593..12895)
                     /locus_tag="VCA20_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEP1835097.1"
                     /translation="MYLETFATHSDSTSRVFNMTKDELQRKSVTALFKNQGIYNGLLA
                     VFLFYGIFTGNLEITSIFVLYVIGAAAYGAITASPKIILTQGGPAILALISILLFK"
     gene            complement(12971..13396)
                     /locus_tag="VCA20_001278"
     CDS             complement(12971..13396)
                     /locus_tag="VCA20_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEP1835098.1"
                     /translation="MQEMEDLLYRLKVADETISNLFEKQLGISLTRYSILQTLLKDAP
                     LHQLALQERLQIDRAAVTRHLKLLEESGYIIRKRNPDNQREVLVWPTEQAREALITNP
                     SAHHQAIKTLMNQILTVEESEQFLATLDKLLIGLQNLPI"
     gene            13558..14316
                     /locus_tag="VCA20_001279"
     CDS             13558..14316
                     /locus_tag="VCA20_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEP1835099.1"
                     /translation="MENTPRLNLVIDVLMLAGAILAQSGAEIHRVEDTMIRIAHSQGI
                     EQPNVLAIPAAIFFSIDHTNISRMKRIVNSNYDMQKVCDVNQISRSLANEEISLEEAW
                     SQLNAIKQQKPPYNNIQITLAAVVAAPFFTIMFGGSLLDAAGAVLATALAFPISLIVE
                     RYIGIDFVTTFVGAFVFSLTSYLLSATTTLPINSNLINAGAVMPFVPGIAFTNAVRDL
                     MTNHINTGMPQFFQTALIILSLGAGTTVAFMLVR"
     gene            14320..14817
                     /locus_tag="VCA20_001280"
                     /pseudo
     CDS             14320..14817
                     /locus_tag="VCA20_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735768.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
BASE COUNT         4499 a         3284 c         2588 g         4521 t
ORIGIN      
        1 agcgatgagg aatccctatt tctatccaaa atcactgggt gaatttggac agaaataagg
       61 attgaaatca tgtttgaatg gagagtatct tgaaggagtt taggacttca aggtttgagt
      121 catctacgaa atgactaagt ggcacaattg tgatcatgct tatagagagt aggactcgcg
      181 gcagaaccat tgacctgtag gtaaccaatc tacgaatatc agagtggtca ttgcccgaag
      241 ctaaaaacta ggctctttct ggatactttt cattttcatt ttttatcagt tcgattgttt
      301 ttacttgaca aagggcttta catatcagtc ataagaaaat atcaatggcg catcataata
      361 gccttcgtca ctaattttta tggttggcca ctccaacaac ttgatttctt tcaaaaatga
      421 caaactagat ttcccgatga aaatcgctat caatctcaaa aaatggttgg atatcctgaa
      481 catactccaa aacgccttga aactctcctt ggtcatcacg cactgcctta tagaccacat
      541 agacaaactt gcccatgctc tcggacttga accacatggt gacctggtct ttttgccctg
      601 tgcggagcaa gtcaaaaatc ttcttgacct tgtccactat tttgggagga tggcatagct
      661 ccacatggcg tccaatctgg cttggcgttc gtttgaaaat catgtcttct acagggtggc
      721 tgtcattgta gtattggaaa atgtcatcat gttcattgct atcttcctag taatccatcc
      781 aatatttcga tcaatttttc tttttcctga ctctcatttg ttttaaacct gtttaggggg
      841 atatcgcatt ttttcaaaat gttatccttc ataatatcac gttcaaactg cttagaatta
      901 cctttgtgga atgcatatcc atctacttct acagctagta caggggcttt ggtaagtcta
      961 ttaattatca aaaaatcaag gtgcgtattt ccatgccttg catacttcgc ttccacttct
     1021 gttagtcgag taaaatcctt cacaagatta tgcagtttat agtcatgtat aacatctaaa
     1081 ctattatatt tttcatcgct cataaacact tccctaatcc gatgatacat taaattctca
     1141 gaatcatatc gcgatatccg ttttcgtttt ctaaaatatt cctccttttc ttgctcatac
     1201 tgtttgtata agtaatcgaa gatggaatat acagggctct ctcttacttc aaaattatta
     1261 tattgtatat atcccaataa atcactaata tttttatcta acgattgctc attcccagtc
     1321 atgacaagaa ataatttatt tttagctctt gatatcgcca catttagtaa gtacggatca
     1381 tccgtaaaac gtgttatgct gttatcaacc gtggagatga ttatcgtatc tttttctttt
     1441 ccctgaaact tatggactgt atcgctttcc aaatcatcaa tttgctcttg caacttctct
     1501 ttctgctttc gataaggcgt gacaactcct gtccgttccc gatagatttc ataatttgga
     1561 ataacatctt tcagaattat atcaatctct ctctgactat agtgatctcg agcgtgattt
     1621 cccttaggtg ttctaatagc ctgaagaaca tctttttccc cattatcacc tgtcataatc
     1681 actaagtcac cattataaaa cttttgattg cagaaattga taattttggg atgacatcta
     1741 taatgctctc gtaatagcgt ttctggaata ttcggaatca cttttagtac cgattgaaga
     1801 aaactatttg tgtattcata accgctatga acaccgtatt tatggaaaag ctgagcacta
     1861 ctttctttga catcttttgt gacaatatgc ggtagttgtt tactatctcc aacaactaca
     1921 acatttttag cacaggataa tgctagtgcc ccagttgtaa tatccacttg tgaggcttca
     1981 tctatgatta aataatcata ggtaatattc tttcctaaac ttgttgttga tgaaaatgtt
     2041 gtactaagaa caataggata ttctagaaga actcgtttcg attgttccct taaatcttcc
     2101 tttgtaaact gtactctatt tcttccattc ccatacttac taactagaaa atctttgaaa
     2161 atttttaatg aatcactggt caaattgttt aaaacttccg taccatttaa gtgaaccaat
     2221 tcttcttgaa gagtttctaa tttattcctt atttctgttt gtttatgctg gtaaaatagg
     2281 ttctgaaatt gtattgtcaa atcagacaat tcttgtttat aaagttccca gtttttaaca
     2341 ccatataata aaaagttttt tatcttaaaa attaaaccta accttttatt ctttttatag
     2401 aattcctcaa attcattcca aagcttaaat aacttcttag aagaataaag gtttaattct
     2461 ataggcgata tatctatgtg ctcaaaaaaa tctttaaaat ggcgaaattc cgtttctaat
     2521 tccgaaagtt cttgtctcaa attggccagt tcttcctgaa tttcaaaaat acgaggaagc
     2581 ctacgaatct tttcatttaa ttccttatca aggctttcca taccattatc ttttaatctc
     2641 caagaagaaa aatcagggta catactatac tgattgagaa taaaattatt tttatttttt
     2701 tcacttccaa ggtaagccac gggaaagtca agaccatagt tcttcagttt ttcgtaaata
     2761 ttatcaatgg ctgcattatt attagaaaca attagaaccg tcttattccg tatcactata
     2821 ttagctataa tattcagaat cgtttgtgtc ttaccagttc caggaggtcc ttgaatgaca
     2881 ctaaattgat ttattaaggc attttttgtt gctttatatt gactattatt gcaaccaaaa
     2941 gggaaaatca gttccccatg aagcggcaag tcattcttct tactgggact tagatagctc
     3001 gctaaagcaa ctgttttgtc tacgtaatcc aattcctcat acctttttaa caagagcaat
     3061 tgatcagtcc cagagtttct cagaggattc agcttagata gttccgtcaa ataactaaaa
     3121 acatgacgac tattcgaatg tgtcaactca ttttgccgta tttctaggtc tctccccata
     3181 tataattttc cagtgccatt tttgtagcgg atgatataag cccgtgagtg cgttccattg
     3241 aattcaaaaa ttgactcgat attatttaat aaaaccccat cttgccttct ataaactcct
     3301 atctttgaag gattcaactg tttggaaaac ttcagcacct caactctctg cacattgtag
     3361 cgataaggtt ggacattatt tttaaaataa atttcaaaac agagcttccc tttgtaacga
     3421 atcgattgaa gcgactcgat attttctgac ttgtcccctc catcaatcaa aattattttt
     3481 ctatctttca ccatatctcc actctcactt tcaatcttaa tcttcctcct ccctcaacgc
     3541 ctcaaacttg gctttcatgg ttgggttatt gcggattagt tttttgacgg agacatcgtc
     3601 tagtttatta ttggctagtc gtagctggtt ttcgctggtg gtcagggctg ccttgacagc
     3661 ttccatccgc ttaatagcct tgtcgatttc atcaatagct ttttggaaat tgttgctggc
     3721 cgattgatag tttttggcaa aagcattctt gaaaatttcc aaatcttctt caaaatgagt
     3781 aatatcaata ttctgctcct taaccaaggc taactcctgc tgatattgca gactattgag
     3841 cgcagcattt cgtaaaattc cgattaattg gataaagaac tgtggtcgaa taacatacat
     3901 cttctcatat tcatgactaa catccacaat ccctgtattg taatagtcat tgtccgcctc
     3961 tagcatgctg actaatacag catattcgca ttttttctcc cgacggtcct tatccaactc
     4021 cttaaagaaa tcagcgtttt tatgcttggt cttggtcgta tctgcctcgt ttttcatttc
     4081 gaacatgatg gaaataaatt ccaagcccga ctcgtcaaaa tcacggaaga tgaaatctcc
     4141 ttttgaaccg gacgctgata cttcattgtc cttcgcaaat ttggcacgtg gaaaagcata
     4201 ggaacgaacc ttgttaaact ccgcctcagc aaattgctcc aagctctctc caattgcctt
     4261 agtagactgc tgggccttga aatttttgta aaattccacc tgctcatccg ccgccttgag
     4321 ccgtacttcg tactcctgac gagtggtcgc cagggctaac tcttgcttct gctcctgcat
     4381 agctaaggca ttctgagccg catcccgctc tttttccacc tgagctaccg cctgctgcaa
     4441 ctccaagtcc ttggcaagac cgatttgccc caactgagcc ttaagctctt ggatttcttg
     4501 gtcttttttg gaaatagcag agctgatttc cagttccgtc tgactagcaa atttatcaag
     4561 ttttgcggtc agttccagaa tctccttatc cttatcactc aatctagcct gcaaatcatt
     4621 ttcagccttc tggcttaata attcccgttc ccgctccaaa cggtcgtgga tttccttgtc
     4681 aaactccgca ccacgcacct gggctaaaag ctgactatat tctgtttcat tgacctgaaa
     4741 ggccgtccca caatgggggc aagtaatttg atgcatatac tctcactttc ttcttttcaa
     4801 ataagatatt aatttatgta aaaccgtagc tactcccagc gccaataaaa aataaaagaa
     4861 acgaaccgca tttctagcat ctggaagtga tagagcccaa aggacaaaaa tcgttatggc
     4921 agtcggccat ctccaggccc aggtcatctt ccgtttgata aaccattcca tccaatcttc
     4981 attagaatca aaccgtgttt cataatattc ttctttcagt tgttcccacc actttttcat
     5041 aacaacctcc tttcagatca cagaccttct aattcaagct ccagaaaagt cctccacttt
     5101 tcctgcaagg gttgacgtac aggtggctcc tgaccttcta agaatttttt gatacattca
     5161 ttttgcacca accagcctgt catgtctttt tgagcattcg caaattcatt accaaaatct
     5221 tctggaattc gttcttcaaa agtaatcaag gtctgatttt ctagctgaga cagtgcaaat
     5281 gaaactgttg ctgaatccca cctgtaggta attttctcat tttttcggta ttccagtaaa
     5341 tccataactt cacggaatcc ttccatttca aatatcaact tgtcaccttc aaccctcaac
     5401 tgcggaaacc agcgagcaaa gccactatcc gtcgccaaca aatcccaagc ttctgtcaaa
     5461 ctagtttgaa cagtatagga aatagttagc agttacatct cggactcttt ctgtacacta
     5521 atcttcatac acaactcctc ttctattccc ccctattata ccaaaaatac tagtccatgc
     5581 cgatatttcc attgtctcta ttctttaagc atgagtacta ttcattccag aggatcgaaa
     5641 aacggtatct tgaaatctga attatttttt ataacatctt tcatagacag tccttacctt
     5701 tgtttatctg gctcaaactc catgttttgt atcccctagc aatgttagac tttcctgcca
     5761 cataatggac attgtcattt ttgaaacact tctatttttt acactttcat ttcagttatc
     5821 cgatacctaa tttattttaa tatatagaac agagtttagt atttcaatca ttcctttctc
     5881 attttatgct atactagatg tattgaaaac aaggaggttt atatgattaa actaattgct
     5941 cttgatatgg acggaaccct tttaaacgaa aaaaaagagc tgatgcagcc acagattaat
     6001 gccatccatc aggctgttga agcgggcgtc aagattgttc tttgtactgg tcggcccttg
     6061 gctggtgtta aacctttcgt agagcagctc ggctttgaca ccgaggaaga atttatcatt
     6121 gtcaataacg gctgttcaac ccattcgaca aaggattggg tcctgattga ctgggaagaa
     6181 ctatctatct ctgatattga ttatctttcc acttttattg agaatgacca tgtacagatt
     6241 tccctcttcg atgagaatga ctatttcgtt cttgcggaaa aagccaatga ccgagtgaac
     6301 ttggatgcta gtttagtcgg tatgataccg cagccaattg acttggacga ggcaacatct
     6361 ggcaaacacc gcttctttga agccatgttt gtcggtgaaa aagtgcatat cgatgccttt
     6421 gaaaatcaac acaatactgt actcagcaaa cgctattcca ccgttcgttc acaggactat
     6481 ctgttggaaa ttttaccaaa cggtgcaagc aaggcaactg gtttaaaaaa actggcagac
     6541 cgcctcggta tcctcccaga agaaatcatg gctataggag atgccaacaa cgaccttgaa
     6601 atgattgaat ttgctggact tggtattgcc atgggcaacg ccaacgtgca ggtcaaggct
     6661 atcgcccagg acatcacaga caccaatgaa aacaacggcg ttgccaaagc cattgaaaaa
     6721 cacattttga acaaataagg agacaatatg aaatacccaa cactcttaga tcgtttcttg
     6781 gtctatgtca aagaaaatac tcgctcagat gaaaattcga caaccacgcc ttctactcaa
     6841 aaccaagtcg agtttgcaca gaatatcctt ctacctgaaa tggaacggat tggcttgcag
     6901 aatgtccact acctgccaaa cggttttgca gtgggaacac tgcctgccaa cgacccaagt
     6961 ttgacccgta agattggctt catcgcccac atggatacgg cggatttcaa cgcagaaggc
     7021 gtgaagcctc agatcatcga aaactacgac ggcaacccaa ttgccttggg aacttctggc
     7081 tacgaattgc atccaaaaga cttcccacaa cttgccaact accacggtca aaccctgatt
     7141 accactgacg gtacaacttt gctaggctct gatgacaagt caggaattgc agaaatcatg
     7201 acggctattg agtacctagt ccaaaatcct gacatcaaac actgcgaaat ccgtgtcggc
     7261 tttggtcctg acgaagaaat tggtgccgga gcagacaagt ttgatgtgga agacttcgat
     7321 gtggactttg cctacactat ggacggtgga ccgctgggcg aactccagta cgaaaccttc
     7381 tcagcagcaa gtgccaagat tgatttccta ggacgcaatg tccacccagg atctgctaag
     7441 gaccagatga tcaatgcctt ccaaatggct attgatttcc acaatgccct tccagaagca
     7501 gaccgccctg agaaaacaga aggctatgaa ggtttcttcc atttgatgaa tatggaaggt
     7561 agtgttgata ctgcttctac cacctacatc atccgcgact ttgaggaaga agatttccaa
     7621 gcccgcaagc aactcatgct ggacattgct gagaaaatga atgccaagtt tgacacaccc
     7681 cgcgtcattg tcaacctgca cgaccaatac tacaacatga aaaaaatcat cgagaaagat
     7741 atgactccaa tcaagattgc taaggacgtc atggaaaatc taggtatcaa gccactgatt
     7801 gagcctgttc gcggaggaac agacggctct aagatttcct tcatgggcat cccaacgcca
     7861 aatatcttcg caggtggcga aaacatgcac ggccgcttcg agttcgtcag cttggaaacc
     7921 atggaaaaag ctgtcgatgt catccttgga atcgtcgctt acaaataaaa tttcaaacgg
     7981 ctttcaatag aaagtcgttt ttttctgaaa aattttaaaa aaatgcaatt ttatattgtg
     8041 ttattgaact aatactgata taatagaact ataaaaaaga aatggagttt tattatgaca
     8101 gaagaaacat tagcacaggg aattctcatt ggcatttggg gtacaacact actattttca
     8161 tttatttggt atattcttgt tgctatttcc aactacatcc tcttcaaaaa agcaggctac
     8221 gctggttgga aatcattgat tccaatctac aatctctata tccaacaatg catcgccttt
     8281 ggctatgaaa aaagatggtt cattctattc ttactgattc cactagcagg accattatat
     8341 ggcatctatc tcgtttacaa ttttggtcgt agcttcggtt tatcagctgt acaagctatc
     8401 ttctatgttc ttcttacacc tatctttaac ctctacatcg cctttaatga tggaagtcgt
     8461 taccaaggtc cacaagaatt ctttattgat taatatatag cggattgaat taggaaaaag
     8521 acaattttaa gttatagtat taataaccca ctcctaaaat aactttagag ttctcaaatt
     8581 gacaaactta tatatgtcct acttctaatt cactcgacta tacaatcaag cccccgcaca
     8641 cgcggaggct tttttaaacg aatagaaaaa taagaagaac catcatgact aggctgatca
     8701 aaatagataa ggagttaaca ttggcaggtt cagctgagcg actacctaac tttaatgcat
     8761 aaattgggga catgactgaa atcggagaga ctagacagac cagcaacatg attttcatcg
     8821 ttttatcaat aggtagaaga aaataaatga ctcctgctat tagaatattc gcacccagcc
     8881 tcaaagagag aagatagacc aatcgactta tttctttcca attcaatttt aaatcaagca
     8941 agattcccaa catgagcatg gctaaaaaag gattggcgtt actggctatc gtcagcgcag
     9001 agataagttc gcgtggaagt ttgatagagc agactgctaa cgataggcta actagataga
     9061 ctactagtgg aactgagcgt gaaagattct ttataaaagt tgcaacggtc attctttcct
     9121 gctcttcatc cacaccagct gcgatactgt aattcccacc tagaaccatg attgcattcc
     9181 ccgcatcaaa tacgatgacg cttagtagac tagatagcgg aaaaaaagct tgcacaaatg
     9241 ggagtgtaaa ggttccgata ttatagcctg ataactgcat aagaccgacg gaagtctgtt
     9301 gaccagactt ctttgcttcc cagtatccca ccatatccaa aaaaagatta caaagaatcc
     9361 ctaatatgaa gggaacgtat aaatctatag atacttgggc tgtcgctgaa gctacaagga
     9421 gagaagctgg tagcgtaata ttcatcacta agctagaaaa gattcttgca tcttcccttc
     9481 gaacaatccc tttgactttt aatgtataac ctaaaccgat aacaaagata aaaccgagag
     9541 cacgggctaa taattcaagt tcattcatga aactagtata gcatatagaa aagaattttg
     9601 atgatatata aaaattttat ataatcgctt gattttgcta aacaataggt atataattta
     9661 tatataaaat aattacataa agataaagag gtgagtttat gtactacccc gtatcctcta
     9721 tcttgattga atttctggtc ttagccatcg tggaaaagca ggattcctac ggctatgaaa
     9781 tcagccaaac catcaagata gttgctgata taaaggaatc cacactctac cctattttga
     9841 aaaaattgga aaaggctggc tatgtcacca cctataccca agaatttcaa gggagaaagc
     9901 ggaaatacta taccattact tcgaatggtc gagaacacct ccccttcctg cagggtgaat
     9961 ggaatagcta caaagaccat attgacggaa ttatagaagg gagcttacga aaatgacacg
    10021 cgctgattat ttgaaccaac tggaacaagc attatttcaa cttcatccaa gtgccagaca
    10081 ggaggcttta gactacttca atgagtattt tgacgaaaag gataacgacg aggaagctat
    10141 tatagagcta ggaacacctg atgacgcagc aaaagaaatt attgccaatc tacctgaaga
    10201 tgccctaatc accgagaaag accaagcaag tccacattca tctaaaccat ttgatttcaa
    10261 ctttgatttt gattttcaat ttgactggga gaattttttc aacaatttca aacattcagc
    10321 ctatcaagct agggaaagaa ttaaattgac gtcgaaaatc gaagaattac ctgagttttc
    10381 aaatctacag atttctctcg aagatcaggc tcttagtgtt caaagttacg acggagaaac
    10441 tgtcttattg cacaatcctg tttcagaaga tggtagctac caggcttttg actatcaact
    10501 ggtccaagat agtttgtaca tatctaacaa gccactccct gaccagctct acttctcaaa
    10561 caatcaaacc agctctgcta ttctcaaaat cccaaaaaga ctcctgcccc ttactagtct
    10621 caatctgaaa acgacagata gtagtctgac aatagtggac gtacaagtat gcgaacagat
    10681 ggtcgttaat gcaaaagata gcagtatcag catgacgaaa gtgagctgcc caagctctgc
    10741 tctacggtta gaagactcta cactaactat ctcagatgga cagatgagcg agttaaaatt
    10801 ggaagccagc gatgctgtta tcactctctc aagcatgagc ctttcagatg caaggataac
    10861 attagaagac tgtgtctgga agttgaagga ttttgtcctt gaaaagagcc ttaactgcca
    10921 ggctgaagat tctgtcttaa cctataaacc aagtacggat atcagtttgg acatctggga
    10981 ggaggattct agcctaaaac ttccaaagga taaggcattt acaatgatga aagaggatga
    11041 catcactcat ctcagctata agcaagagaa tagccctgct cagctggtta ttcattgtca
    11101 agactgccag cttactgttg gataaaggag gtttccatga atcgcaagga atatttacag
    11161 aagcttgaaa aacatctgaa gcacctgcca aaagaagatt ttcaagatac cttggactat
    11221 ttcaatgagt ttttcgatga gaaagaaaac gacgaggaag caattcaagt gctaggcagt
    11281 ccaaaagaag ctgcccgtga aattctagcc aatctctatg ataaagagaa gattgaggat
    11341 aagccaaata cccgcaatat ggtctggttg accatcctcg ctatccttgc cgccccaatc
    11401 ggcatccccc tagccatcag cttggtcgcc ctcttcatca tccttctcct aatcatcttt
    11461 agcctctttc tgctccttat ctcactctgg attgtcctcc tcagtctagc aatcgcccag
    11521 ctactcctcg ccttcgacct attcacacta tcatggtcta cctccctcct cttcacaggc
    11581 ctggccctca tctgcttggc attcaccctc ttaggtagta aatttactct agaccttgga
    11641 agcaaaacct tcttgcatgt tttacattgg atacaaagaa agatccgcaa aggaggctat
    11701 tatgaaacag cttaaaccat ttacgaaaat cagtctgagc ctgctaggtc ttggagccat
    11761 tctaagcctc attggctacc tggctggcgg ttggacaaat atgaaaactg ttgcagaaaa
    11821 ctattttcag ccccaaacgg caagctttac ggatattcgt tctatagagg tcaatgaaaa
    11881 actgtccatt atgccctcgc ctgacaacca attccacctc cactactatc gaaatcagca
    11941 taaagacatc gacctcctca gtcaccaagt ccaagatgga aaattaacca tttctagtgc
    12001 ctatcaaggc atcgttactt atgatggttt actcgaacct atcttaaatc ttgtcaatga
    12061 ccagaatgtt gcctactacc aacctgtctt acaggtccct aaaaatagca caatcgaaac
    12121 actctcaggc aaccttcaag gagatgtcta tctggataaa gtgactattc aagatttcca
    12181 actctttgtt gaaaatggtg acatgttgat tgaaaacagt cacatcagcc ttagtaggct
    12241 attacacatc aatggagaag tagcaatcaa tcagtccata ttaacaagtc cagcggacat
    12301 caccgatact accggaagta ttctaactgt tgaggatggt gattttaaag cagaaaacct
    12361 aaaactagag ggaaggcatg gcatcagtaa ttacaatggt tcgattgaca tttctcttca
    12421 ttccaaaaca aaaactaaca tccatataga tgccagtcaa aataatctag gaattgacct
    12481 tccaacctat tccaatcccc aagccactaa ttacctctat atctctacac tagatggaga
    12541 actgaatatt cagtaattgc aaaaagccct cctcaacgga aggcttttct atttatttaa
    12601 acaacaaaat agaaattaat gccaagatag ctggtccacc ttgggtcaaa atgatttttg
    12661 gacttgctgt aatagcacca taagctgctg ctccaataac gtatagaaca aaaatgcttg
    12721 taatttctaa attaccagta aaaataccgt agaacaagaa aaccgccaac aagccattat
    12781 aaattccttg attcttgaac aatgcagtca cagacttacg ttgtaattca tcctttgtca
    12841 tattaaagac acgactagta gagtctgaat gggtcgcaaa ggtttccaaa tacatgatat
    12901 ataggtgctc aatagctacg atactagcta gaatagtgta aaaatagaca taatgtctcc
    12961 tctcaaaaaa ttatataggt agattttgca agccaatcaa tagtttatca agagtggcta
    13021 ggaattgctc gctctcctca acagttagaa tctgattcat caaagttttg atagcttggt
    13081 ggtgggcaga aggattcgtg attagcgctt ccctagcctg ctcagttggc cagacaagta
    13141 cttctctctg attgtcagga tttcgcttgc gaatgatata gccgctctcc tccaacaatt
    13201 tgagatgacg cgtaaccgct gcgcggtcta tctgaagccg ctcctgcaaa gccaactgat
    13261 gcaagggtgc atcttttagt aatgtttgca atattgaata gcgtgtcaaa ctgattccca
    13321 actgcttttc aaaaagattg gaaatcgtct catctgccac tttcaatctg tataacaaat
    13381 cttccatttc ttgcattatc tacctctttt gattgattaa tcaatcgttg ataagtcaat
    13441 tatactacaa tccgaacaat tttcaactat tttgcctctc tattaaaaaa atatgttgcc
    13501 tacacctata ttttgttata attaagaaaa taactagcag agggatggga cttacttatg
    13561 gaaaacactc ctagactaaa cttagtcata gacgtgctca tgctcgctgg tgccattttg
    13621 gcacaaagcg gtgcagaaat tcaccgagtt gaagatacca tgattcggat tgcacattca
    13681 caaggaattg aacagcccaa tgtccttgcc atccctgctg ctatcttttt ctccattgac
    13741 catacgaata tttcgcgtat gaaacgcatt gtcaattcaa attacgatat gcagaaagtt
    13801 tgtgacgtca accaaatttc tcgttcccta gccaatgaag aaatcagcct agaagaagct
    13861 tggagccaat taaatgcaat caaacaacaa aagccacctt ataacaatat ccaaataacc
    13921 ttagctgccg tagtagccgc accttttttt accatcatgt tcggaggttc tcttttagat
    13981 gcagcaggtg ctgtcctcgc aacagcttta gctttcccta tctcactaat tgtcgagcgc
    14041 tatattggaa ttgattttgt caccaccttc gttggcgcct ttgtcttctc actcacctct
    14101 tatttattaa gcgcaaccac tactctcccc attaattcta acctgattaa tgcaggggca
    14161 gtcatgcctt ttgtaccagg gattgctttt acaaatgccg taagagacct catgaccaat
    14221 catatcaata cagggatgcc ccaatttttc caaacagcgc ttattatctt atcgctcggt
    14281 gcaggtacga ctgtggcctt tatgcttgtg aggtaatcta tgacaccagt tcacttctta
    14341 ttacaggtac tcgcctctta tatcgctatt gcagccttct taatcgttct caatgttcaa
    14401 agaaaaatgc tggtccctgg tggactccta ggcatgcttg tttggcttat ttatctttta
    14461 ctattggaac caacaaatgt cttgattgct accttttttg cggcaattat cggatcctgt
    14521 gtcagtcaaa tcatgagtat ttggttaaaa acaccctccg tcatcttttc attggctata
    14581 cttgcaccac ttgttccagg ctattgagcc tacatgacca cgacttattt cgtttctggg
    14641 gtccgcgctc aagccctaac caatatcact accgtcttaa ctttagcctt agtcattccc
    14701 atcggtatgg ccagcggaac aatattatta cgcctttata aggttcttcg aacaggcaaa
    14761 aaagcagcac gccaaacgca tgaagaaaaa aacgttttca gaaaacgaaa atcataacca
    14821 aacatcaatc aaagtagtac actagaatag aggtgaacta caatgattga ttttctttta
    14881 tccatagaag ag
//
LOCUS       DATUCG010000054        14846 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.055, whole genome shotgun sequence.
ACCESSION   DATUCG010000054 DATUCG010000000
VERSION     DATUCG010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14846
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.055"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(<1..297)
                     /locus_tag="VCA20_001281"
     CDS             complement(<1..297)
                     /locus_tag="VCA20_001281"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEP1835100.1"
                     /translation="MNKQEAIEKIKNIDTLNINDIIAGQSVDMVIKNQVLDIVSQIHQ
                     PQKVVVPKIAAEWIKSHKESFSDASAIDMYDNLTSDNRSGYYHEVWLWVIAHHYD"
     gene            complement(290..481)
                     /locus_tag="VCA20_001282"
     CDS             complement(290..481)
                     /locus_tag="VCA20_001282"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835101.1"
                     /translation="MAFIHLDGNWQNVKVDNLFEVTTEELKLFHKRKHERLSPELTKA
                     FFLRIRLENTLRKLEETDE"
     gene            complement(481..1047)
                     /locus_tag="VCA20_001283"
     CDS             complement(481..1047)
                     /locus_tag="VCA20_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835102.1"
                     /translation="MTDIVDIYMKCGDFHTAVKQSGLPIHEAHLKLLKSGCLKIQDKI
                     RYGSKGAKLGGQAEELFQTLVPDAVDANRYFQKNNPVYDFILKDVTIDVKYSSLHIDK
                     RYGTRKSHWSVGARGEQDFICAFLERAEGEKLNDPIILLLPMAFIEVKKDLHISQSGA
                     WMKEFQIEPEELQSTLKEYVALREQGLF"
     gene            complement(1040..1288)
                     /locus_tag="VCA20_001284"
     CDS             complement(1040..1288)
                     /locus_tag="VCA20_001284"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835103.1"
                     /translation="MVRNTIEDVNNLLVAQLEKLSFDDISPEELELEVTRSKAMVHLT
                     DEILKTQVLALRVVETLSEVTSKEVIPPKMLLGGGVND"
     gene            complement(1299..1730)
                     /locus_tag="VCA20_001285"
     CDS             complement(1299..1730)
                     /locus_tag="VCA20_001285"
                     /inference="COORDINATES: protein motif:HMM:NF013579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEP1835104.1"
                     /translation="MVMKLVKITELASIERSSGKVYPAGCTLIQISATRGQVLYHAEE
                     KEIPSHYAVFLPNDKVLPKYLYHAISYQAGRFIHTVQTGLNIQVGTLNEMKLKIHTDL
                     EKQAEIVRYLDVIEKMEAKEEATIELLKQAKQTQLSEMFAG"
     gene            complement(1724..2809)
                     /locus_tag="VCA20_001286"
     CDS             complement(1724..2809)
                     /locus_tag="VCA20_001286"
                     /inference="COORDINATES: protein motif:HMM:NF014442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HEP1835105.1"
                     /translation="MNREAILEICGVKEVFEVPQTLMTKLFSPEQSDLLARIASIYGD
                     RQLDQFRDFFQEEGADRKKLKQDYTPDGVAELLARVSRGGKSLADICAGTGSLTIQYL
                     NYHPDVEFVRCEEFSARVIPFLLINLAIRKIDAEVIHGDSLTREQFAVYAIQDGVISQ
                     IDSPSDRKVDAVISNPPYSMPWTPISDERFDLYGLAPNLFLNTGIPVVILLLRKGRSQ
                     KDVFFVDAKDEFTKGKAQNSLDVEHIKKIVSVVSLRMTTERFSYLADWEELVENGFNL
                     NIPRYVDTFVPEEVQPLGVILRELIETDKKIAETEREFARLFGQLVATEPTKQAELEA
                     EQELMLEYVDKPSLSELIKESEQLTLW"
     gene            complement(2874..3131)
                     /locus_tag="VCA20_001287"
     CDS             complement(2874..3131)
                     /locus_tag="VCA20_001287"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835106.1"
                     /translation="MYKLDFVVNGGWIFPIGVYETKEDVKQAIYWYIYSYSAIQRPVF
                     RTSGSDDVKRVDYGACDCYFLVKAELDCMAVEYHRTKHGGS"
     gene            complement(3132..3350)
                     /locus_tag="VCA20_001288"
     CDS             complement(3132..3350)
                     /locus_tag="VCA20_001288"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835107.1"
                     /translation="MEGLDKVKRVILKHGLKQDNPFVRDVRHSTTGLEIFYGNERQDF
                     RYANWQVGVVMTKHLYLHGNFKIIEVED"
     gene            complement(3371..4144)
                     /locus_tag="VCA20_001289"
     CDS             complement(3371..4144)
                     /locus_tag="VCA20_001289"
                     /inference="COORDINATES: protein motif:HMM:TIGR01446.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HEP1835108.1"
                     /translation="MANANKRYYWIQLAQDFFKSKEMKLLRKIAGGDTHTIIYLKMML
                     LSLEDNGILFFDGVADNLAEEIALVIDEDVENVKITLVFLQSKKLLSKISDREYFLEQ
                     VPEMVGSETASARRVRKHRENQKVLQSNSDETNGNGEKEKEQEKDIDININLSSSSCI
                     NNSDYSIKQLFKDFEAGFGRLLSPFEIEDIQKFATEEGLSPELIREALKEGVFRNKPV
                     WNYIKAILRNWKNDKLLTVELVRARQQEQELPKNVDVSP"
     gene            complement(4128..4304)
                     /locus_tag="VCA20_001290"
     CDS             complement(4128..4304)
                     /locus_tag="VCA20_001290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835109.1"
                     /translation="MVWIVAKKTKTRRGYRFYQKRSFNTWQKARIYQQDLFNKGVNAE
                     MWEENGGIEIGKCK"
     gene            complement(4307..4540)
                     /locus_tag="VCA20_001291"
     CDS             complement(4307..4540)
                     /locus_tag="VCA20_001291"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835110.1"
                     /translation="MKFLEMMKRFLSLDEVEDSVNKVNPLTSSEEKWKALALELNKEL
                     QRTREQHKFAIQECVRLQTICDCQAELLAEREV"
     gene            complement(4547..4735)
                     /locus_tag="VCA20_001292"
     CDS             complement(4547..4735)
                     /locus_tag="VCA20_001292"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835111.1"
                     /translation="MIEELMLTTEQGLAFIAILTPILIWLIRKPVEIEIEVKEPIVEI
                     KQPERNLRYLQIRTYYGG"
     gene            complement(4744..4983)
                     /locus_tag="VCA20_001293"
     CDS             complement(4744..4983)
                     /locus_tag="VCA20_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEP1835112.1"
                     /translation="MENIMTMAWAEKSEMFDPDNAYPPIMTQTEFKKWLKIGDPFLKY
                     LISKGMPVIRNENGSIRIPRDAVRVWLRDNWQVLA"
     gene            complement(5081..5788)
                     /locus_tag="VCA20_001294"
     CDS             complement(5081..5788)
                     /locus_tag="VCA20_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HEP1835113.1"
                     /translation="MNEIFVFHGQEVRTVTINNEPWFVGKDVADILGYSKSRNAIALH
                     VDEEDALKQGIPTSGGIQEMIIINESGLYSLILSSKLPQAKEFKRWVTSEVLPQIRQQ
                     GAYVPENLSDEAFIALFTGQKKLKEQQLVLAQDVDYLKNEQPIHPSFAQALLKKRKAR
                     VVACLGGMDSPAYTDKVFAQSVFRQAEVDFKDHFNINRYDMLPKKFAEAALSYWMTWE
                     PSTNTKMKILEMNAYEL"
     gene            5845..6276
                     /locus_tag="VCA20_001295"
     CDS             5845..6276
                     /locus_tag="VCA20_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017865021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835114.1"
                     /translation="MNSVLTDFLKAFNDITAPIGFIITICTFFLARATKDKLDESKEI
                     GLFSEEANQYLGRLNAIKILLNQIDNRFATVPEDIVKNVSDIVSEIEHSYPTLSKKNK
                     VFSKPIKQFKKLHRYQFVEYINFIDPFNALHSILSNRRDLK"
     gene            6276..6605
                     /locus_tag="VCA20_001296"
     CDS             6276..6605
                     /locus_tag="VCA20_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017865022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835115.1"
                     /translation="MNPSIDLLCQLTVEKKLSWKTIDKLLVNGIPYSIQFQHILPDKS
                     FFTEINSKIFIVLYGEVRDFLSDRIKKGYYLQTLTDNTIEKIDAPEVDVVKLHTLITI
                     LNDFSNS"
     gene            complement(6841..7038)
                     /locus_tag="VCA20_001297"
     CDS             complement(6841..7038)
                     /locus_tag="VCA20_001297"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835116.1"
                     /translation="MKIAEKVVRIESDAYEYVVDFANEHDLKIGEAVSILIRYCASKD
                     LIVKQAHVEVVEVQNVVEEDD"
     gene            complement(7043..7255)
                     /locus_tag="VCA20_001298"
     CDS             complement(7043..7255)
                     /locus_tag="VCA20_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEP1835117.1"
                     /translation="MSQQHQKWIDLVKKRLHERGWSQSDFAIVLGVSPAMVTRLLKEG
                     HGSDDLKLKVNKKLRISESWESFEEE"
     gene            7317..7523
                     /locus_tag="VCA20_001299"
     CDS             7317..7523
                     /locus_tag="VCA20_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835118.1"
                     /translation="MKQFILDCIGDRDDFTITITFSNGDKIDFFQVYDDCSETANAID
                     LVEVGTDFRHLVNLDYVVHVRLNV"
     gene            complement(7535..7735)
                     /locus_tag="VCA20_001300"
     CDS             complement(7535..7735)
                     /locus_tag="VCA20_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835119.1"
                     /translation="MKEIIEKHFENMVDEILSASETYEEAISSLKKISVLGIHQPGGL
                     IKSLEIAIKRRACQQKTPNHKD"
     gene            complement(7899..8165)
                     /locus_tag="VCA20_001301"
                     /pseudo
     CDS             complement(7899..8165)
                     /locus_tag="VCA20_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017354.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8245..8418
                     /locus_tag="VCA20_001302"
     CDS             8245..8418
                     /locus_tag="VCA20_001302"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835120.1"
                     /translation="MSKTFPEMATEITSSLHEELYQAYVQKNNFDPSFQKDFGEYVGE
                     QFQHILKSLNAKN"
     gene            complement(8401..8541)
                     /locus_tag="VCA20_001303"
     CDS             complement(8401..8541)
                     /locus_tag="VCA20_001303"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835121.1"
                     /translation="MEKRTLDEVIVQYVADVIKESDKKDPATIAAVAELIKVTHLLIL
                     SI"
     gene            8829..9212
                     /locus_tag="VCA20_001304"
     CDS             8829..9212
                     /locus_tag="VCA20_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP1835122.1"
                     /translation="MFSTFERIKELCKKHGISLNALEEKLGYSRNTIYSLKKQKPNAE
                     RIAEIADYFNVSTDYLLGRTDNPRIASDETVDNNISPAETQLLAAFRNQTDHMTEEDK
                     FRFNKALESLMTTAKTLIDDDSKWR"
     gene            9215..9973
                     /locus_tag="VCA20_001305"
     CDS             9215..9973
                     /locus_tag="VCA20_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEP1835123.1"
                     /translation="MARKIILLRQYIQHWDNAYQVIDAISALYRIPHAQITFDHVIRH
                     FENTYNVRFVYFDRDPFPELPTAGFLGAEHIRYRGLVSNPDVIQSNEILCRHNDGITL
                     YDKEKDKYVVFINQTNIKERVIFTILHELAHIEAHFSTGHSEAIALACTSEYQSSPLE
                     EEANTMASLFFINNDRLVWHLRNSHSYYDIKKMNTISDNALFNRLVDFVHYRIFTYEE
                     HLLDEQQQRQAAINLVKKFKQGNNLLQKYYDIDV"
     gene            10160..11176
                     /locus_tag="VCA20_001306"
     CDS             10160..11176
                     /locus_tag="VCA20_001306"
                     /inference="COORDINATES: protein motif:HMM:NF019371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HEP1835124.1"
                     /translation="MKPFSDIEEQLRILQEDKKLLIQDMDNAIFALMNCGYYEIVNGY
                     KIFLLDPNEEDEVFKKGETFEHLASLYNLDKELRNYVMQATLEIENSLKTALAYTIAE
                     DFGVYEIDYLNRTNYVKGKWQKHHQNYQRDHLLDMLKDIAANRKVEPLISYRVKHGHI
                     PPWILFKEATFGNFVNLFKVLKGPQKNKVIAHCIGLDINLLTEDDKRLFGEMLDLVLA
                     FRNRAAHGGRIFNYKAKRNTALSYHSKFHPQIDISPAGYRHGKGRNDFYTFFYSMVIF
                     DNKNGHNYLNFCLYKIIEHEKEYPSDLEILERQMGFPQGRLYNEIEKLNQTINLYRLL
                     NDKN"
     gene            11418..12479
                     /locus_tag="VCA20_001307"
                     /pseudo
     CDS             11418..12479
                     /locus_tag="VCA20_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194677.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(12551..12622)
                     /locus_tag="VCA20_001308"
     tRNA            complement(12551..12622)
                     /locus_tag="VCA20_001308"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12587..12589),aa:Arg,seq:ccg)
     gene            complement(12660..13853)
                     /gene="rpsA"
                     /locus_tag="VCA20_001309"
     CDS             complement(12660..13853)
                     /gene="rpsA"
                     /locus_tag="VCA20_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEP1835125.1"
                     /translation="MNEFEDLLNSVSEVTPGDVVTAEVLTVDAGQANVAISGTGVEGV
                     LTLRELTNDRDADINDLVKVGETLELLVLRQVVGKDTDTVTYLVSKKRLEARKAWDKL
                     VGREEEVVTVKVTRAVKGGLAVEFEGLRGFIPASMIDSRFTRNTERFVGQEFDAKIKE
                     VDPAENRFILSRRDVVEAEAAAARAEVFGKLVVGEVVTGKVARITSFGAFIDLGGVDG
                     LVHLTELSHERNVSPKSTVTVGEEVEVKVLAIDEVEGRVSLSLKATQPGPWDGVEQKL
                     AAGDVIEGKVKRLTDFGAFVEVLPGIDGLVHISQISHKRVENPKDALSVGQEVTVKVL
                     EVNAADERVSLSIKALEERPAVAEGEEKAEKRAPRPRRQKRDYELPETQSGFSMADLF
                     GDIEL"
     gene            complement(13977..14048)
                     /locus_tag="VCA20_001310"
     tRNA            complement(13977..14048)
                     /locus_tag="VCA20_001310"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14014..14016),aa:Gln,seq:ttg)
     gene            complement(14431..14511)
                     /locus_tag="VCA20_001311"
     tRNA            complement(14431..14511)
                     /locus_tag="VCA20_001311"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14475..14477),aa:Tyr,seq:gta)
     gene            complement(14563..14796)
                     /locus_tag="VCA20_001312"
     CDS             complement(14563..14796)
                     /locus_tag="VCA20_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEP1835126.1"
                     /translation="MSKKDKKIEIQIEDSKVLVNREEFPGYRLVIGKKVIGEIAELAE
                     NNFAIIKNGNTESFYKKLEKAVGNIIENYNLSH"
BASE COUNT         4504 a         3430 c         2463 g         4449 t
ORIGIN      
        1 atcgtagtgg tgagcaataa cccaaagcca cacctcatga taataaccac ttctgttatc
       61 tgaggtgaga ttatcataca tatctatagc agacgcatcc gaaaatgatt ctttatgact
      121 ctttatccac tccgccgcaa tcttcggcac cacaaccttc tgcggttggt ggatttggga
      181 gacaatgtct aacacctgat ttttaataac catgtcaacg ctttgacctg caattatatc
      241 gttaatattt aacgtatcta tatttttgat tttttcaatc gcttcctgtt tattcatctg
      301 tttcctccaa ttttctaagt gtattttcca atctgattcg caaaaagaat gctttggtca
      361 attcaggcga caaccgttca tgctttctct tgtggaaaag cttcagttct tccgttgtca
      421 cctcaaaaag attatccacc ttcacgttct gccaattccc gtccaagtga ataaaagcca
      481 ttaaaatagc ccttgttcac gtaacgctac atactcttta agcgtagact gtagttcttc
      541 cggttcgatt tgaaattcct tcatccaagc accactttga ctgatgtgta aatctttttt
      601 gacctcaata aacgccatag gcagtaatag aataattggg tcatttaatt tttctccctc
      661 tgcacgttcc agaaatgcac agataaaatc ttgttcccca cgagccccta cgctccaatg
      721 cgacttcctg gttccatagc gcttgtcgat atgtaacgag ctatatttaa catcaatcgt
      781 cacatctttc aggataaaat catagacagg attatttttt tgaaaatacc tattggcatc
      841 cactgcatca ggtaccaagg tctgaaataa ttcctctgcc tgtccaccta attttgcacc
      901 ctttgaacca tagcggattt tatcctgtat tttcaaacaa ccgcttttta aaagtttcaa
      961 atgagcctca tgaattggta agcctgattg ttttactgct gtatgaaaat cgccacactt
     1021 catgtaaata tctacaatgt cagtcattta ctcctccacc tagtaacatt ttcggaggta
     1081 ttacctcctt gctcgtgact tctgacaagg tctcaaccac acgcaaagcc aagacctgag
     1141 ttttcagaat ttcatctgtc aaatgcacca tagccttgct acgagtgact tctaattcca
     1201 attcttcagg agatatatca tcaaaactta atttctccag ttgggctact aagagattgt
     1261 ttacatcttc aattgtgttt ctgaccatct gtatcctcct agcccgcaaa catctcgctt
     1321 agctgtgtct gctttgcttg ttttaataat tctatggtcg cctcttcctt agcttccatc
     1381 ttctcaatca catctagata ccttacaatt tccgcttgct tctccaagtc cgtatggatt
     1441 ttcagcttca tctcgttcag ggtacccacc tggatattca gtcccgtctg gacagtatgg
     1501 atgaaacgcc cagcttggta agaaatagca tgatagagat acttgggcaa taccttgtca
     1561 ttcggcaaaa atacagcgta atggcttggg atttcctttt cttcagcatg gtaaagtact
     1621 tgccctctcg tcgcgctaat ttgtatcaag gtacagccag caggataaac cttgccactc
     1681 gaccgctcta tgcttgccag ttctgtaatt ttgactaatt tcatcaccat aatgttaatt
     1741 gctcgctttc tttgattaac tcggaaaggc taggcttgtc cacatactca agcattaact
     1801 cttgttctgc ttccagctct gcctgcttgg ttggttctgt tgcaaccagt tgaccaaaca
     1861 atctggcaaa ctctctctcc gtttcagcaa ttttcttatc tgtttcaatc aactccctca
     1921 agattacccc taagggttgg acttcttcag ggacaaaagt gtcgacatag cgagggatgt
     1981 tcaggttgaa cccattctcg acaagctcct cccagtcagc tagataagaa aaacgctctg
     2041 ttgtcattct caaggacacc accgaaacaa tcttcttgat atgttccaca tccaaactgt
     2101 tctgtgcctt gcccttggta aattcatcct tggcgtctac aaagaataca tctttctgac
     2161 ttcgtccctt tctcaagagc aaaataacaa ctgggatacc tgtattcaaa aacaggttgg
     2221 gtgctagccc gtagaggtca aaccgttcat cactgattgg tgtccatggc attgagtaag
     2281 gtgggtttga gatgactgca tcaaccttcc tgtcgcttgg actatctatc tgactgataa
     2341 caccatcctg gatagcatag acagcaaact gttcccgtgt caggctatcg ccgtggataa
     2401 cttcagcgtc aatcttgcga atagctaagt taatcagcag aaacgggata acccttgcag
     2461 aaaattcctc acacctcacg aactccacat ctggatgata gttcagatat tggatagtca
     2521 gactgcctgt ccctgcacag atgtccgcta ggctcttgcc acctctggac accctcgcca
     2581 gcaattccgc tacaccatca ggcgtgtagt cctgtttcaa ctttttacgg tcagccccct
     2641 cttcctgaaa gaagtcacga aactgatcca gttgcctatc gccatagata gaagcaatgc
     2701 gagcgagtag gtcagactgc tcagggctga acagtttggt cattagtgtc tgcggaactt
     2761 caaaaacctc tttgacccca catatttcta aaatagcttc tcgattcaac tcccccgccc
     2821 tttcaaatac tctggcatct cctgcccgat ttcgatttct tcgtcatgag ccatcaacta
     2881 cctccatgct tcgtccgatg atactccact gccatacaat ccaactccgc cttcaccaaa
     2941 aaataacaat cacaagcacc ataatccacc cgcttcacat catccgaacc actagtccga
     3001 aagactgggc gttgaatagc cgagtaggaa tagatgtacc agtagatagc ctgcttgaca
     3061 tcttccttgg tttcgtagac acctatcgga aagatccagc caccattcac aacgaaatcc
     3121 agcttgtaca ttcagtcctc cacctctata attttaaaat tcccatgcag atacaagtgc
     3181 tttgtcatta cgacaccaac ctgccaattt gcgtaccgaa agtcctgacg ctcattgccg
     3241 tagaaaattt ccaaaccagt tgtcgaatgc cttacatcac ggacaaaagg attgtcttgc
     3301 ttaaggccgt gtttcagtat gacccgtttt accttgtcta atccttccat aagttcatag
     3361 cctccaaaaa ttaaggcgaa acatcaacat tcttaggcag ttcctgttct tgttgcctag
     3421 ctcgaacgag ttctactgtc agtaacttat cattcttcca atttcgtaaa atcgctttga
     3481 tataattcca tacaggttta ttgcgaaata ccccttcttt aagagcttcc cttattaatt
     3541 cagggctaag cccttcttca gtagcaaatt tctggatgtc ctcaatttca aatggactta
     3601 ataatcttcc aaagccagct tcgaaatctt tgaataattg cttgattgaa taatcgctat
     3661 tatttataca actactacta gataagttta tatttatatc tatatctttc tcttgttctt
     3721 tctctttctc tccgttacca tttgtttcat cgctgttact ttgtaacact ttttggttct
     3781 cacgatgctt gcgaacccta cgggcacttg cagtttcact acctaccatc tctgggactt
     3841 gctccaaaaa atactcccta tccgatattt ttgataacag ttttttggac tgtaaaaaaa
     3901 caagggtaat ttttacattt tccacatctt cgtcaattac cagcgcaatt tcttcagcca
     3961 agttatcagc gaccccgtca aaaaatagaa tcccgttgtc ttccaggctc aacagcatca
     4021 tcttaagata gataatcgtg tgagtgtccc ctcctgctat ttttcgaagc aacttcattt
     4081 ccttggattt gaagaaatcc tgtgcgagtt gaatccaata gtaccgctta tttgcatttg
     4141 ccaatctcta tacctccatt ctcctcccac atctcagcat ttacaccttt attaaacaag
     4201 tcctgctgat aaattctagc cttctgccaa gtattaaacg accgtttttg gtaaaatcta
     4261 taaccacgcc tagtcttggt tttctttgca acaatccaaa ccatagctag acctcacgct
     4321 ctgccaatag ctcagcttgg caatcgcaga tagtctgcaa tctgacacat tcttgtatag
     4381 cgaacttgtg ctgttcacga gtgcgttgaa gctctttgtt taattccaaa gccagtgcct
     4441 tccacttctc ttctgaactc gtcaaagggt taactttatt aacagaatcc tcaacctcat
     4501 ctaaactcaa aaaacgcttc atcatttcca aaaatttcat attatcttat cctccgtaat
     4561 atgtccgaat ttgcaagtat ctcaaattcc gctctggttg ctttatttcc acaataggct
     4621 ctttgacttc aatctcaatc tcaacaggct tacggatcag ccagattaag attggggtca
     4681 aaatagcaat aaatgccaaa ccttgttctg ttgtcaacat caattcttct atcatatcct
     4741 ctcctatgcc aaaacttgcc aattatcacg tagccagaca cgaacggcat cccttggaat
     4801 gcgaatactc ccgttttcat ttctgattac aggcatgcct tttgaaatga gatatttcaa
     4861 aaatggatca ccaattttca accacttctt aaactcagtt tgggtcatga ttggcggata
     4921 agcgttgtca ggatcaaaca tctcagactt ttcagcccat gccatagtca tgatgttttc
     4981 catcttttct ttaaaaaagc tttcaaaatc aatcataata tgttacactt tctttgtatc
     5041 tttatatttg agcctgattg ccgtcaggct tttttgcgtt ttacagttca taagcgttca
     5101 tctccaaaat tttcattttg gtgttagtgc ttggttccca cgtcatccag taagacaatg
     5161 ccgcttcagc aaatttcttt ggcaacatat catagcgatt gatattgaaa tggtctttaa
     5221 aatcaacctc agcttgtcga aagaccgact gtgcgaagac tttatcagta taggctggac
     5281 tgtccattcc acctagacaa gcgacgacac gggcttttct cttcttcagc aaggcttgag
     5341 caaagctagg atgaattggt tgctcatttt tcagataatc aacatcttgt gctaatacca
     5401 actgctgctc tttcagtttc ttctgcccag taaatagagc gataaaagcc tcatcagata
     5461 aattttctgg cacataagct ccctgctgac gaatctgagg caatacctca cttgtgaccc
     5521 aacgtttaaa ttctttggcc tgtggaagct tgctagataa gattagcgaa taaagaccgg
     5581 attcattgat gataatcatt tcttgaatgc caccgcttgt agggatgccc tgttttaggg
     5641 cgtcctcttc atcaacatga agtgcaatcg catttcgtga ttttgaatag cctaaaatat
     5701 cagccacatc tttcccaaca aaccatggct cattgttaat cgttacagta cggacttcct
     5761 gtccgtggaa aacaaaaatt tcgttcataa aattcctttc taatttggta taatagagat
     5821 aaatagtata ttgaggtaga taatatgaat agtgtattga cagatttcct taaagctttt
     5881 aatgatatta ctgcaccaat tggattcata atcacaattt gtactttttt tctagccaga
     5941 gctacaaagg acaaacttga cgaatcgaaa gaaattggat tgttttcgga ggaagctaat
     6001 caatatctag gcagattaaa tgctattaag atattactga atcagattga caaccgtttt
     6061 gcaaccgttc cagaagacat agtcaagaat gtatctgata ttgtctctga gatagagcac
     6121 agctatccaa cactttctaa gaaaaataag gtattttcaa aaccgataaa acaatttaaa
     6181 aagttacatc gctatcaatt tgtcgaatac atcaatttta tcgacccatt caacgcactt
     6241 catagcatac tttctaacag aagggactta aaataatgaa tccatcaata gacctccttt
     6301 gccaattaac cgtagaaaaa aaactatctt ggaagacaat tgataaacta cttgtcaatg
     6361 gaattccgta ctctatccaa tttcaacaca ttctgccaga taaatctttt tttactgaaa
     6421 ttaattcaaa aatcttcatt gtgctatatg gtgaagttag ggatttcctt agtgacagaa
     6481 tcaagaaagg ctactacctg caaacactga ctgataacac cattgaaaag attgatgccc
     6541 ctgaagttga tgttgtaaag ttacacacct tgattactat tcttaacgat ttttccaatt
     6601 cttaatctta tttgaaaaat atcgatattc tttcattagc tttttaatag caaagaaatt
     6661 taaaacaagc acaacaatgg ttaatattaa cttgagcaac tccaaataaa acactattag
     6721 taccccctcc ttatctctcc aagtgccacc acagtccccc acacatctaa cccctcaagg
     6781 ctatcaacta tcagttgact aagctggtag tttttctttt gctagttctg gattagtttg
     6841 ctagtcatct tcttccacca cattctgcac ttccacaacc tctacatgag cctgcttgac
     6901 tatcaaatct ttagaagcac agtagcgaat caggatgctc actgcctcac cgattttcaa
     6961 gtcatgctca ttggcgaaat caacaacata ttcatacgcg tccgattcaa tacggactac
     7021 cttttcagct attttcatgt ttctactcct cttcaaaact ttcccagctc tcagaaattc
     7081 tgagtttctt gtttactttc agcttgaggt catcgctacc atgcccctct ttcagcaaac
     7141 gagtgaccat ggctggtgaa acaccaagca caatcgcaaa atccgactgc gaccaaccac
     7201 gttcgtgtag tcgcttttta accaaatcaa tccatttttg atgttgttgg ctcatatttt
     7261 ccctttctat ttttggtata attgacttga aatctttagt gaaaggagga caagtcatga
     7321 aacaatttat tctcgattgt attggtgacc gtgatgattt tacaatcaca atcactttct
     7381 caaacggaga caagattgac tttttccaag tctatgacga ctgctctgaa actgctaatg
     7441 ccatagacct tgttgaagtc ggaacagatt tccgtcatct agtcaatctt gactacgttg
     7501 ttcacgttcg actgaacgtt tgatttcttg acacctaatc cttgtggtta ggtgtttttt
     7561 gctgacaagc cctacgcttg attgcgattt ctaaactttt gataagaccg cctggctgat
     7621 gaatgcctag gaccgaaatc tttttcaagc ttgaaatagc ttcctcatag gtttcagatg
     7681 ccgacaaaat ctcatcaacc atattctcaa aatgtttttc aatgatttct ttcatccctc
     7741 tctcctttct aattttgcat tgcaacagaa ggaaacactc attgctatca cagcctacat
     7801 tacttctctt gagctgttga aagagtatca tgaatggctg aaccaatagc ctggatggtt
     7861 tttgagccaa gcggtaaaac gctagcctct ttaatttcat ctcccaatac aatcttgtgt
     7921 tgggactttt cattgtcaat ataggcactt gccatttttc taactcgact tgcaaccatc
     7981 tcacaaatcg tggctgtttc tactggaaga tctggatact tatcaaaata aaacgtacga
     8041 gtttccaatt cggacaagtt atcacactcc ttcaaaagtt ccagaaatgc cgattcagca
     8101 atggtatcaa taagtttgac agttgtttca atgacaggta aaaaatttgt tgtgagtttt
     8161 ttcattccct tctcctttct aatcttgttc ccctccccta tccatgctat aatttagcta
     8221 ttaacacgga aaggagggaa aactatgagt aaaacttttc cagaaatggc tactgaaata
     8281 acttcctcgc tgcatgaaga gttatatcag gcctatgttc aaaagaacaa tttcgaccct
     8341 agtttccaaa aagactttgg cgaatatgtc ggcgaacagt tccaacacat cctaaaatca
     8401 ctaaatgcta agaattagta gatgtgtgac cttaatcaac tcagcaactg ccgcaatagt
     8461 tgctgggtct tttttatctg attccttaat aacatccgct acatattgga ctattacttc
     8521 atcaagtgtt ctcttttcca tctcctcccc tcctttccat aaaattagtt aaaaagttag
     8581 taaaatcgtt gacatttaac aatggataat ttaaaatata accatagaga aaaagcttga
     8641 taaaaaccag ttcaatcaat acgttctgct cgccaaagct tttatttttt gaattagttt
     8701 taacttgcgt tttcactaac tctttaactt acaaaaacta ttttaagtta ttcgttgtgt
     8761 tttgtcaata gtttgcaatg aaaaatttaa atattttttg tcaatctctt agaaaggttg
     8821 ataaatcaat gttttcaaca tttgaaagaa ttaaagaatt gtgtaaaaag catggaattt
     8881 cactcaatgc acttgaagaa aaactcggat atagccgaaa tacaatttat tctttaaaaa
     8941 agcagaaacc aaacgctgaa cggattgcag aaatcgctga ctattttaat gttagtacag
     9001 actacctgct tggacggaca gacaacccac ggattgccag tgatgagaca gtagacaata
     9061 acatttctcc tgctgaaaca caattactag ctgccttccg aaatcaaaca gatcacatga
     9121 ctgaagaaga taagtttcgt ttcaataagg cattagaaag cctaatgaca acagctaaga
     9181 cccttattga cgacgatagt aaatggaggt aacattggca cgaaaaatca ttctgctaag
     9241 acagtacatc cagcactggg acaacgctta tcaagttatc gatgccattt cagcattgta
     9301 tcgtatccct catgcacaaa taacatttga ccatgtcatt cgacattttg aaaataccta
     9361 taatgtgcgc tttgtttact ttgatcgtga cccgtttcca gaactaccaa ctgcaggctt
     9421 cttaggtgct gaacatatca gatacagagg tctagtttct aatccagatg tcattcaaag
     9481 taatgaaatt ttatgtaggc acaacgacgg tattacctta tacgataaag aaaaagacaa
     9541 gtatgtagtt tttataaacc aaacaaacat caaagaacga gttatcttta caatcctaca
     9601 cgaactggct cacattgaag cacatttcag tactggtcac tctgaggcta ttgccttagc
     9661 atgtacgagc gaatatcaaa gtagcccatt ggaagaagaa gcaaacacta tggcttccct
     9721 gttctttata aacaatgacc gtcttgtctg gcatttgaga aatagccact cctactacga
     9781 cattaaaaaa atgaatacta tcagcgacaa tgcccttttt aatcgactag ttgattttgt
     9841 ccattatcgg atctttactt acgaagaaca cctcttagat gaacaacagc aacgacaagc
     9901 agcaattaac cttgtgaaga aattcaaaca aggcaataac ttactccaaa agtattacga
     9961 tattgacgtc taacgggaaa gagatggacg acattgacca ggcacttgct aaacactggt
    10021 aaaaataaca taatttactt gacaaaatat aaaagaggct ctataataga atcatacgaa
    10081 gtggcccgga tgcccgatgc atcctaccga aagagtctca atttatttga ggctctttcg
    10141 tgctttataa ggagttacca tgaaaccatt ttctgatatt gaagaacaac ttcgcattct
    10201 tcaagaagat aaaaagctac tgatccaaga catggacaat gccatctttg cgctaatgaa
    10261 ttgtggatac tatgaaatcg tcaacggata caaaatcttc ctactggacc caaacgaaga
    10321 ggatgaagtc tttaagaagg gcgagacctt cgaacacctt gcctccctct ataatctcga
    10381 caaagaactg cggaactatg tcatgcaggc cacgttagag attgaaaatt cattgaaaac
    10441 agcccttgcc tacaccattg cagaagattt cggagtatac gaaattgatt atttaaatcg
    10501 caccaactac gttaagggaa aatggcaaaa acaccaccaa aactaccaac gggatcattt
    10561 gctcgatatg ctgaaagaca ttgctgctaa ccgtaaggtt gagccactaa tctcctatag
    10621 agtaaagcat gggcatatcc ctccttggat actcttcaaa gaagccacct ttggaaactt
    10681 cgtcaacctc ttcaaagtcc ttaaaggacc gcagaaaaac aaagtcattg cacactgtat
    10741 cggcttggac atcaatttac taacagaaga cgacaaaaga ctgttcggag aaatgttgga
    10801 cctcgttctc gccttcagga atagagcggc acacggcgga cgaatcttta attacaaagc
    10861 caaaagaaac accgccttat cctaccactc aaaattccac ccacaaatag atatatcccc
    10921 tgccggctac cgacacggaa aaggacgaaa tgacttctac acctttttct actcaatggt
    10981 cattttcgat aataaaaacg ggcataacta tttaaacttc tgtctctata aaattattga
    11041 acacgaaaaa gaatacccaa gcgacctcga aatattagaa agacaaatgg gtttcccgca
    11101 aggcagactc tataacgaga tagagaaact taatcagacc atcaatctct acagactact
    11161 caatgacaaa aactagttta tttgatacaa tacagttacc aacccaggag tttcctagtg
    11221 caaaaacgga gttggcacaa gctgccacat ctgagcggta actattcgta gttgccgctt
    11281 ttattttaca acagacaaaa aagccctacg ctcaactttg gacggtccga gcgtaaggca
    11341 aatcatgtat agtaaaaacc tgctttgagt aggtctcttt actataccca ttttatcaaa
    11401 aaagaaaggg taaatcaatg gtatcttata gaaaacgtag taacggatgg gagtaccgga
    11461 tatcatataa aaaaccagat ggcacctacg gcactcttcc aaagggtgga tttaagacca
    11521 aagctgatgc tgtcattgct gcttcccaag ctcaaatcga cctagagaat aatgttatag
    11581 tcgataagaa cataactctc gcagattatt ttgatcagtg ggccacggta cacaagaaac
    11641 ctcacataga tccagtcaca ttcgaaaagt acgagtttac ccacaaaaaa atcttagaat
    11701 acttccctga tgtcaagatg cacaaaataa ctcctacgag ctaccagaat gtgctaaatt
    11761 tgatggctga tagatttgtc cactcgacca ttaaactctt caaccagcac atcagaggag
    11821 ccctcaaagt agcagtttat gaaggaacct tgaagaagga ttttacaact cttgccaaag
    11881 tacattccaa tgttgattct aaggacgagg cacacaagtt tattgaattg gaagagtacc
    11941 tggaactaat agctcactcc agaaaaaata tcaagcacca gtcccacttc ttcatctacc
    12001 tggtatccaa aacaggacta cgacctgcag aggctcagtg actgactaca gatgatatgt
    12061 atgacatact agagttagac atcaacaaga cttacaaaat cagcggtaaa aacaaaggtt
    12121 ggaaagggac caagaacaag caatcacaac gtagagtccc ttatgataat gatgtctggg
    12181 aagcatatca agaatatctc aaaacaggct acatagacaa cccagagaag cggcttttta
    12241 tcagagcctc tgacacagct attaaaaagg ttattaggaa gagagtcagc aaccagttag
    12301 caacaccgca cagccttcga catacctatg tcagctatct caaacacaaa ggaatagaca
    12361 tcctaaccat ctccaaactg attggccata aagatgtact agaaacactc aagacctaca
    12421 cccatctatt ccaagaccag aaagaagatg actttgagaa aatcagacaa ctattctaaa
    12481 actttttgga caaattctgg acaagataca aaaaaagccc gatttaacgg gctttttaag
    12541 gtggttctaa tccaccctga gggaatcgaa cccccatctc aagaaccgga atcttacgtg
    12601 atatccatta cactaagggt ggaaaggaaa aacttgcaca aggcaagttt ttatatgaat
    12661 tacaattcaa tgtcaccgaa aaggtcagcc attgagaatc cagattgagt ttctggcaat
    12721 tcgtaatcac gtttttgacg acgtggacgt ggagcacgtt tttcagcttt ttcttcacct
    12781 tcagcaactg ctggacgctc ttccaaagct ttgatagaaa gtgatacacg ctcatcagct
    12841 gcgttcactt caagaacttt aacagttact tcttgaccta ctgacaaagc gtcttttgga
    12901 ttttcaacac gtttgtgtga aatttgtgag atatgtacca aaccatcgat acctggcaat
    12961 acttcaacga aagcaccgaa gtcagtcaag cgttttactt taccttcgat gacatcacca
    13021 gctgccaatt tttgctcaac gccatcccat ggaccaggct gtgtagcttt caatgacaat
    13081 gatacgcgac cttcaacttc gtcgattgca agaactttaa cttcaacttc ttcaccaact
    13141 gttacagttg atttaggaga tacgttacgc tcgtgagaca attcagtcaa gtgtaccaaa
    13201 ccatctacac caccaaggtc gatgaatgca ccgaagcttg tgatacgtgc aactttacca
    13261 gtcacaactt caccaactac caatttaccg aatacttcag cacgtgcagc cgcagcttct
    13321 gcttcaacaa cgtcacgacg tgacaagatg aaacggttct ctgcaggatc aacttctttg
    13381 attttagcat caaattcttg acctacaaaa cgctcagtgt tacgagtgaa acggctgtcg
    13441 atcattgaag ctgggatgaa accacgaaga ccttcaaatt caactgcaag accaccttta
    13501 acggcacgag ttactttaac tgtaacaact tcttcttcac gaccaacaag cttgtcccat
    13561 gctttacgtg cttccaaacg ttttttagat acaagataag taacagtgtc tgtatcttta
    13621 ccaacaactt ggcgaagtac taacaattca agtgtttcac caacttttac aaggtcgttg
    13681 atatcagcat cacggtcgtt agtcaactca cgaagagtca atacgccttc aacaccagta
    13741 cctgagatag ctacgtttgc ttgaccagca tcaactgtca atacttctgc agttaccaca
    13801 tcaccaggtg taacttcgct tacactgttc aacaaatctt caaattcgtt cattataaaa
    13861 aaacctccga caatcaggtc tttcgaccca acataaaata tattttattt aaggcacacg
    13921 caaggacaac aacaattaga catctttgac gaactagact atatctagta cctttactgg
    13981 ggtagctgga ttcgaaccaa cgcatgaggg agtcaaagtc ccttgcctta ccgcttggcg
    14041 ataccccaaa aaatagatac aattcattta tttactaatc acaagaaatt gaactcgaga
    14101 ttaatttctt agaaaaatag atagccttcc ttgagtgtta acactcagca tcagtctcct
    14161 aattttctgc agaaatttct ggtaaaccag ttcatctctc atatcttaaa tgaactcagc
    14221 ctaaaagctg ggtaaaatag accgatttcc ttgtgtcctg cgacacggtg tcaaccgcct
    14281 aatttcactg ggcgttttaa cgacttcgta tcttagttga acacggacta aaatcctact
    14341 gaaaaagata aacttccttg tgtgtttagc acactgcgtc agtttcctat tttcatacgg
    14401 atttcttaac gtcctttgta tcctgttcaa tggagagaga gggattcgaa cccccgaacc
    14461 cgaaggagcg gatttacagt ccgccgcgtt tagcctcttc gctatctctc cgtgcactaa
    14521 cagtcactat tctaacataa actagcgact attacaagcc ttttagtgac ttaaattata
    14581 attttcaata atatttccga ctgctttttc taattttttg taaaagcttt cggtatttcc
    14641 atttttaata attgcaaagt tattttcagc caattctgca atttctccaa ttactttttt
    14701 acctataaca aggcggtagc cagggaactc ttctctattg acaagaactt tgctatcttc
    14761 aatctgaatt tcaatttttt tatctttttt actcattttc aaacctcact tttactattc
    14821 tacaaaaaaa gagaagagct tgcaag
//
LOCUS       DATUCG010000055        14413 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.056, whole genome shotgun sequence.
ACCESSION   DATUCG010000055 DATUCG010000000
VERSION     DATUCG010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14413
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.056"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            <1..100
                     /locus_tag="VCA20_001313"
     CDS             <1..100
                     /locus_tag="VCA20_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEP1835127.1"
                     /translation="EKTWAHIKKHLRRVLPNCDTFLEALSSCSCFG"
     gene            complement(179..940)
                     /locus_tag="VCA20_001314"
     CDS             complement(179..940)
                     /locus_tag="VCA20_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEP1835128.1"
                     /translation="MSHQLENAKNLYLRGIRDGEIKEVHENYMGSSYTQHSTGVPDEK
                     EGFAAFFEDFFKRNPKREITIARAIEDGNFVFVHVHQKLNDGEAEWVTADIFRSDENG
                     RIVEHWDVIDAYPKNLGQTDPIYADFELTDLDKTEDNKKIVRRFLVDVLQNGEIEKFD
                     DYVAADLIQHNQEIAQGGAAYKDYLVDKAVDYDFVFKVMGQGDYVVAYSKVWIAGQDY
                     AHFDIYRLKDGKIVEHWDNKEVMPEKKDLTNLGKF"
     gene            complement(952..2463)
                     /locus_tag="VCA20_001315"
     CDS             complement(952..2463)
                     /locus_tag="VCA20_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEP1835129.1"
                     /translation="MNLFETFIERKDEWGVALFEHLRISLLALIIAIAIAVPLGLILS
                     SKKRLTEWSLQITGIFQTIPSLALLGLFIPFMGIGTLPAVVALVIYAIFPILQGTLTG
                     LGEIDPSLEEAATAFGMNKWEKLKKFKLALAMPILMSGIRTASIMIIGTATLAALVGA
                     GGLGSFILLGIDRNDSALILIGAVSSAVLAVLFGYGIRLLQDKKPKTILLALLVTLFT
                     VGASYVPMLNFSTKQLVIAGKLGAEPEILINMYKLLIEDRTDIKVEIKPNFGKTSFLY
                     EALKSGSIDIYPEYTGTITSTLLKNSPMDLSTNPEEVYTYAKEAILEQDGLMYLAPMA
                     FQNTYALAVTEDYAQKNGIEKISDLAKVQQTAVAGFSLEFNDREDGNIGLKNLYNLQL
                     NVKTMEPALRYEAIKSGNVQIIEAYSTDSKVVTYKLKILEDDKQLFPPYQAAPLLTKE
                     TLDKYPELEQVLGALAGKISTEEMTQMNYAVDVEGKSAEQVAREYLEKENLLK"
     gene            complement(2463..3194)
                     /locus_tag="VCA20_001316"
     CDS             complement(2463..3194)
                     /locus_tag="VCA20_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1835130.1"
                     /translation="MIDYQNVSLTCKVSGPILKNLTFDIQEGEFFVLIGPSGSGKTTT
                     LKLINRLIEQTEGEVFFQGKRLKDFDLRELRLETGYVLQQIALFPNMTVAENIALIPE
                     MKGLGKEETLTRTRELLTKVGLEPDSYLDRLPKDLSGGEKQRIGILRAIIANPKVLLM
                     DEPFSALDPISKAQLQDLIKELHEEFKMTTVFVTHDMDEAVKLADRICLMQDGQVVQL
                     GSPDELRNHPANDFVKEFIRARGGM"
     gene            complement(3187..3636)
                     /locus_tag="VCA20_001317"
     CDS             complement(3187..3636)
                     /locus_tag="VCA20_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEP1835131.1"
                     /translation="MPEVRKRSKDYARMFDQQVGLYEGYARRHGLNAKCLSILMWIYY
                     NPGGVTQNWVSKKTYSSKQVVNATVKKFLDGGLVVLEENPADKRHKKIKLTEEGQQFA
                     SRILDPLEEAENKALSQLSQEEQELMLGLIQRYSQSLMAILKGEDDD"
     gene            complement(3946..4482)
                     /locus_tag="VCA20_001318"
     CDS             complement(3946..4482)
                     /locus_tag="VCA20_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEP1835132.1"
                     /translation="MVKKSSSVWTSLLLGAAGGAAAAAFLASKTGKTVKEKVVNFAND
                     YKENHEEINADFVTKAQDLGKQATERFTEVKTQLETGELTVEDLVKSGKEKSLETFEQ
                     IKEKIAEQNLSTADILEAIKAKTAKAPTVDLTEEDIEDAVVVSEDIEIDIEDVQTEVV
                     SETVSEVASETASETVEG"
     gene            complement(4466..4879)
                     /locus_tag="VCA20_001319"
     CDS             complement(4466..4879)
                     /locus_tag="VCA20_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEP1835133.1"
                     /translation="MIIEISVLIIALSIAAVAVYIVLLLKKLGTVTDEAQQTLKVLTS
                     DVNVTLYQTNELLAKTNVLVEDVNGKVSTLDPLFVAVADLSTSVSDLNASARDLTVKA
                     KSAGASTVKAGGALSALSTLSSLLGKKGEKNGKKI"
     gene            complement(4890..5693)
                     /locus_tag="VCA20_001320"
     CDS             complement(4890..5693)
                     /locus_tag="VCA20_001320"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEP1835134.1"
                     /translation="MNTIEKRLNMDSIAVKLGPLEIRWYAICILLGLILGVYLATKEG
                     PRKKIRQDDILDFILIAFPLSIIGARIYYVAFSWNEYKDNILSIFAIWNGGIAIYGGL
                     ITGAIILYFFTQYRFINTLDFLDIVAPSVMIAQAIGRWGNFFNQEAYGKAVESLNYLP
                     AFIRDQMYIDGAYRQPTFLFESLWNLLGFGLVCVLRRRPKFLKQGEITAFYLVWYGCG
                     RLLIEGLRTDSLMFLGIRVSQWLSGVLILLGIIMVVLRRRKSSIPFYQP"
     gene            complement(5668..6600)
                     /locus_tag="VCA20_001321"
     CDS             complement(5668..6600)
                     /locus_tag="VCA20_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEP1835135.1"
                     /translation="MTVYVKDLVDNLRIECAYSTEELLQKQITTADITRPGLEMTGYF
                     DYYAPERIQLIGMKEWSYLMAMTAHNRYQVLSQMFQPETPVVIVARGLEIPEEMYKAA
                     KEKQIAICRSKTATSRLSGELSSYLDSRLAQRTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDVYAKDDVTLWGEPAEILRHLLEIRGVGIIDIMSLYGAS
                     AVKDSSEVQLAVYLENFETGKVFDRLGNSGDTIEIAGIAIPQIRIPVKTGRNISVVIE
                     AAAMNYRAKQMGFDATKIFEERLSNLIEHNREEA"
     gene            6815..7759
                     /gene="manA"
                     /locus_tag="VCA20_001322"
     CDS             6815..7759
                     /gene="manA"
                     /locus_tag="VCA20_001322"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019323776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEP1835136.1"
                     /translation="MMEPLFLTPVMHEKIWGGNRLRTNYHYDIPSDKTGECWAISAHP
                     NGVTTVSNGQYRGRGLDDLYKNEKHLFGNPTDDVFPLLTKILDADDWLSVQVHPDDSY
                     GLAHEGELGKTECWYILEAEDGAEIIYGHNAQSKEELRQQIEAGDWDKLLTHVPVKKG
                     DFFFVPSGTMHAIGKGILILETQQSSDTTYRVYDFDRRDDAGNLRELHIEKSIDVLTI
                     GPVANSTPAHLKAGNLDSTLLVANPFFTVYKWNIQQEIKMEQTVPYLLVSVIEGEGTI
                     QVGETSYPLQKGSHFILPANVTDWTFTGQMNIIASHTN"
     gene            complement(7907..8179)
                     /locus_tag="VCA20_001323"
     CDS             complement(7907..8179)
                     /locus_tag="VCA20_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEP1835137.1"
                     /translation="MSIELYKVRQGKIVSGVLAGIAHKLGWESWVTRAIFIAGLLLGK
                     SFLLLIALYIAAAYFLPYKEDRDAERYGTGPRKIKEAEKINKSWFE"
     gene            complement(8169..8930)
                     /locus_tag="VCA20_001324"
     CDS             complement(8169..8930)
                     /locus_tag="VCA20_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835138.1"
                     /translation="MTQKREERLIELYEKGVLTKEEARACFKEMNQEPDFLFVEEKVK
                     LNFTLPSFKVFASSKLKQSYSFEGIESLFLKLSEGRLTLAKSKNNQISVEICYNQDAP
                     EDKLPRLYVEKKGLYFHSSLACRLTINLPQEWMSVLDLELGQADARLDYLPFEDISIR
                     SSRDKKQQDIRLTTCGGYPQHLYVQLAQAPLTLHTGKGQGIRGQIESQSGHVLVNRKK
                     KNSPYQFEKSGNDLLFLKVQTGQGGFYVKGIKDVN"
     gene            9128..9568
                     /locus_tag="VCA20_001325"
     CDS             9128..9568
                     /locus_tag="VCA20_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEP1835139.1"
                     /translation="MRKLVNEVLFIGTGKSKFSVLEFGEFEILGSTSVIGYDNSYDYE
                     GDFILTARVGANAGELYRHSGKVKISDNTVFIQGDLLDFIFYILQHFDIKKLSFGTGQ
                     PLVKASELKGLKIIMPVSVKERNHVGTFFSTLDRHITLHQRKSI"
     gene            complement(9572..10495)
                     /locus_tag="VCA20_001326"
     CDS             complement(9572..10495)
                     /locus_tag="VCA20_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003036835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEP1835140.1"
                     /translation="MNKITRETLLHEYFKEWTRLFKEGAVRPITLEKYRNNHYHLKNI
                     VPDLVLHQLDRTTYQQILNDYALTHERQTVIDFHHQLKGAVLDAVDEGYIDRDPTRKV
                     IFKGKAPRIKKMKYLNQFELHNVLSDLDLECRINWDWLILLIAKTGLRFSEALGLTPA
                     DFNFVQQNVSVTKTWNYKGNGGFLPTKNKSSMRKVQIDWQLSMQFQQLIKDLPQDKPI
                     FVNGSVCNSTPNKWLERHCKNVGVPVISIHGLRHTHASLLMYAGVSIASVSRRLGHSS
                     MTTTQKVYMHVISELENQDTDLVMRYLSGLS"
     gene            complement(10510..11079)
                     /locus_tag="VCA20_001327"
     CDS             complement(10510..11079)
                     /locus_tag="VCA20_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002415016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEP1835141.1"
                     /translation="MKCHPPPLWDRVKNTPAWELRKLGKVARFRRGSFPQPYGNSEWY
                     DGDGAMPFVQVADVTDEMNLVGDTKQKISRKAQPLSVFAEEGSVLVTLQGSIGRVAIT
                     QYEAFIDRTVLIFENFNLPVDNLFWAYIIKQKFIEEAKCAPGGTIKTITKEVLSDFDI
                     KLPSHPEQSAIGSFFSTLDRQITLHQRKS"
     gene            complement(11164..14289)
                     /locus_tag="VCA20_001328"
     CDS             complement(11164..14289)
                     /locus_tag="VCA20_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017868658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEP1835142.1"
                     /translation="METISKNASEREFQDKFVRELSKFKWQTPEHLNGNLQKVTVQDL
                     INNWRSELNRMNADVLEGVALTDSEFDQVMTKVKQISNSYEAAKILSMEGSTGKIDGI
                     YRDQHPHVTKKQITLTILKKAQVSGGDSSYQVAREVWSDNGNRFDLVLLINGLPLINI
                     EQKRSDKSLDEAFGQFKRYYADGEYTNNFMSFSQMMVVTTDIETRYFATPKSINDFNP
                     AFVFHWSVTKKDQASGKHLGQRVLTDYREVIEHFLMVPMAHQMVGDYLIINEDTENEE
                     NRRHMLMRPYQVYAVQAVEMAAFGWDNAEKIPHGGFVWHTTGSGKTITSFKTAMLLSS
                     KTDAAKIVFLLDRRDLDAQTAKAFKAYATYDFVTVDETKSAYQLRKTLSDSHKGIVVT
                     TTFKLSNLVKDLIDGDDRTLADKKIIFIIDEAHRTTMGDMMGMIKDYFYKNGLFFGFT
                     GTPLFDENKVKGKINERSELINTTEKLFGPLLHQYTIDEAIADGNVLGFHVDYINTGE
                     FRSYEDLREDLIELLTEKHPEKAVKEIERQVMRLSELDVEKHAAKEGLLVYHNQTHIP
                     RVVEEILSGWQEQSQGGEFNAMLTVAFKERVIAYYHEFKKQIAEQFTDGAVKPNIAMT
                     FSFGNENDPDNVAREVIEEMFADYEGFTGIRFIAGDKKKGEDAYFEDVTDRAKRGGSG
                     RNPKNIDILIVADQLLTGYDAKRLNTLYVDRKLELQGLIQAYSRTNRVFGSSKEFGTI
                     INFQYPRITEEIVKEALLLYGSGGSNSRVLVEKYGVAVDLLRTKVEALRSELPNPTQW
                     IDIQQDEEKKGTFKTAFKETFKQLDRVKQYYEFVWDDEAFGLDEHSWLQYIGAYRNLF
                     PRGTGGDDWVPKDLKGRTKLQGSQIIDAQSLFALIGNKTTNEGGFQVINDNNLVLILE
                     QIQELSDLGENELAEALKTFINEELVTGQLSASVDFDVAFKEWQENRQFAVIKEFAGQ
                     WGIDSDLLRKSLQEYNTSKPDDIPYREEIATTLDPHQATNEFAGNKLKLNMNLNKVLP
                     QWMAETKQKYK"
BASE COUNT         4115 a         3349 c         2654 g         4295 t
ORIGIN      
        1 cgagaaaaca tgggctcaca tcaaaaaaca cctcagaaga gtattgccga attgcgatac
       61 gtttcttgag gcactttcgt cctgctcttg tttcggttga ctatatcata aaaggaatat
      121 ttttacaatg gaaaaagcag actttctcaa acgagactgc ctgctctgct tttttccctt
      181 aaaacttccc taaattcgtc aagtctttct tctctggcat gacttccttg ttgtcccagt
      241 gctcgacaat ctttccgtct ttgaggcggt agatgtcaaa gtgagcgtag tcctgacctg
      301 caatccagac tttcgaataa gccaccacat agtcgccctg tcccatcact ttgaagacaa
      361 aatcgtagtc gacagcctta tcgaccagat agtccttata ggccgcaccg ccttgggcga
      421 tttcctggtt gtgctgaatc agatcagctg ccacatagtc gtcaaatttc tcgatttccc
      481 cattttgaag gacatcaacc aagaaacgac ggacaatttt cttgttgtcc tcggtcttgt
      541 ctaggtcagt cagctcaaag tctgcgtaaa tcgggtctgt ttggccaagg ttttttggat
      601 aggcatcgat aacatcccaa tgctcgacga tacgaccatt ttcatccgaa cggaagatgt
      661 ccgccgtcac ccactcagcc tccccgtcat tgagtttctg gtggacatgg acgaagacaa
      721 agtttccgtc ttcaatggca cgggcaatgg tgatttcccg cttgggattg cgtttgaaaa
      781 agtcctcaaa gaaagccgca aagccctctt tctcatcagg cacacccgta ctgtgctggg
      841 tatagctgga tcccatatag ttttcatgaa cttccttgat ttcgccatca cgaatgccac
      901 gaaggtaaag atttttagcg ttttctagtt gatgtgacat agtattttct cctattttag
      961 tagattttct ttctcgaggt attccctcgc cacttgctct gccgacttgc cttccacatc
     1021 gaccgcatag ttcatctggg tcatttcctc ggtcgagatt ttgcccgcta aagcccctaa
     1081 aacttgctcc aactcgggat atttgtctaa ggtttctttg gtcaatagcg gagcggcctg
     1141 ataaggtggg aagagctgct tgtcgtcttc caggattttc agcttgtaag tgaccacctt
     1201 gctgtctgta gaatatgctt cgataatctg cacattacca ctcttaatag cttcataacg
     1261 aagagctggc tccatggtct tgacattgag ttggagattg tagagatttt ttagaccgat
     1321 attaccatct tcgcgatcgt taaactctaa tgagaatcct gcgacagctg tctgctgtac
     1381 ttttgccaaa tccgaaatct tttcaatgcc atttttctga gcataatctt ccgttacagc
     1441 caaagcatag gtattctgga aggccattgg tgctaaatac atcagaccat cctgttccaa
     1501 aatcgcttct ttagcgtagg tataaacctc ctcaggattc gttgataaat ccattggtga
     1561 atttttcaaa agagtacttg taatggtccc tgtgtactca ggataaatat caatagaacc
     1621 tgatttgagt gcctcataaa ggaagctagt ctttccgaaa ttaggtttta tctccacctt
     1681 gatgtctgtc cggtcctcaa tcaacaactt atacatattg atgagaattt caggttctgc
     1741 accgagcttc cctgcaatga ccaactgttt tgtggaaaaa ttaagcatgg gaacataact
     1801 agctccaaca gtaaataaag tcaccaatag agccaataaa attgtcttgg gtttcttgtc
     1861 ctgcaacaga cgaatgccat aaccaaaaag cactgcaagg actgccgacg ataccgcacc
     1921 aatcaaaatc agggctgaat cattgcggtc aattccaagt agaataaagg agcccaggcc
     1981 cccagccccc accaaggccg ccagagtcgc cgttccgata atcataatag aagccgtccg
     2041 aatccctgac atcaaaatag gcatggcaag agctaactta aattttttca acttctccca
     2101 tttattcatg ccaaaagcgg ttgctgcttc ttccagagaa gggtcgattt ctccaagtcc
     2161 tgtcaatgtc ccctgcaaga taggaaagat agcataaata acaagtgcta ctactgctgg
     2221 caaggttccg attcccataa aaggaatgaa aagaccaagc aaagcaaggg atggaatagt
     2281 ttggaagata cctgttattt gcagactcca ctcggtcaag cgttttttac tagatagaat
     2341 cagtcccaaa ggtactgcaa tggcaatcgc aataatcaaa gctagaaggg agatgcgaag
     2401 gtgttcaaaa agagcaactc cccactcatc cttgcgttct ataaaggttt caaataggtt
     2461 cattacatac ctcctcgagc tctgataaat tccttgacaa aatcattggc aggatgattc
     2521 ctcaattcat caggacttcc taattgcaca acttgtccat cttgcatgag acagatgcga
     2581 tccgccaatt taaccgcctc atccatatca tgggtaacga aaaccgttgt catcttgaat
     2641 tcctcatgta attccttaat caagtcctgc aactgagcct tactgattgg atccagagct
     2701 gagaaaggtt cgtccatgag taacactttc ggatttgcaa tgatggcccg caaaatccca
     2761 atccgctgtt tttctcctcc agacaagtcc ttgggcaaac gatccagata gctgtctggt
     2821 tccaagccaa ccttggtcaa gagctctctc gtcctcgtca aggtttcttc cttgcccaaa
     2881 cctttcattt caggaataag agcaatgttt tcagctaccg tcatatttgg aaaaagggct
     2941 atctgctgaa ggacataacc tgtctccaaa cgtaattcac gcaggtcgaa atccttaagt
     3001 cgcttacctt gaaaaaaaac ctctccctcg gtctgctcaa ttagacggtt aatcaatttc
     3061 aacgtagttg tctttccgct accactcggt ccaatcaaga caaagaactc cccttcttga
     3121 atatcaaagg tcaaattttt caagattggt ccgctaacct tacaggtcag ggatacattt
     3181 tgataatcaa tcatcatctt ctccttttaa aatagccatg agactctgac tgtagcgctg
     3241 aattaatcct agcataagct cctgttcctc ctgactgagt tgagacagcg ccttattttc
     3301 cgcctcctct aagggatcca agatgcgact agcaaattgt tgcccctcct ctgtcaattt
     3361 aattttttta tgtcgcttat ctgctggatt ctcctctaaa actaccaggc ctccatccaa
     3421 aaatttcttt acagtagcat tgacaacttg tttgcttgaa taggtcttct tactgaccca
     3481 gttttgcgtc acacctccgg gattataata aatccacatg agaatggata aacattttgc
     3541 attgagccca tgccggcgag cgtagccctc atagagcccg acttgctggt caaacatacg
     3601 agcgtaatcc ttacttcttt tcctaacttc tggcatcgtt cccctcccta ttaagcacaa
     3661 tcaccttgcc aaaagcctct ggtctactag tgttcaaaac aacaacaatt ttcacaacga
     3721 cttcctcctg taatcatgtt agtcaatatt ttttactatt ttaccaaaaa aagaaaaata
     3781 gtcaatcatt ttgactattt tgaaacatca actaccttat tatagattcc cgtcttcatc
     3841 cattcaaaca tattaaatcc ctgaaattat ctgcttttaa tttacacaaa acaaatgata
     3901 atcagataac aaaactagcc tagaatcttc taagctagtt tctttttacc cttcaactgt
     3961 ttctgaggca gtttcgcttg ctacttctga aacagtttct gataccactt cagtttgaac
     4021 atcctcgata tcaatttcaa tatcttcaga cacaactaca gcatcctcaa tatcttcttc
     4081 tgtcaaatcc actgtcggag ctttagcagt ttttgccttg atagcttcca aaatgtcagc
     4141 cgttgacaag ttttgttccg caattttttc cttgatttgc tcaaatgttt ccaaggattt
     4201 ttccttacca gacttgacca aatcttctac tgtcaattca ccagtttcaa gttgagtttt
     4261 tacttcagta aatcgttcag tcgcttgttt gcctaaatct tgtgccttag tgacaaaatc
     4321 agcattgatt tcttcatggt tttctttata gtcattagca aagtttacaa ctttttcttt
     4381 gactgtttta ccagttttac ttgctagaaa agcagctgca gcagctccgc ctgctgcccc
     4441 taacaaaagg ctagtccaca cgctactaga tttttttacc atttttttct cctttcttac
     4501 ctaagagaga tgagagagtt gacagagctg aaagagctcc gccggctttt acagtacttg
     4561 ctccagcaga cttagctttg actgtcaaat cacgcgctga agcattcaaa tctgatacag
     4621 aagttgacaa gtcagctacc gctacaaaaa gaggatcaag ggtcgaaacc ttaccattta
     4681 catcttcgac taagacattg gttttagcca aaagctcgtt ggtctgatag agagtcacat
     4741 tgacatcgct ggtcaagacc ttaagcgttt gctgggcttc atctgtcacc gtacccaatt
     4801 ttttcaacaa caaaacaata tatactgcca ctgccgcgat ggacaaggcg ataatcaaga
     4861 cagaaatttc aataatcata tcttctcctt tagggttgat agaatggaat agaagacttt
     4921 cttctccgca acacaaccat gataatacca aggaggatta acaccccgga tagccattgt
     4981 gaaacacgta ttcccagaaa catgaggctg tccgtccgca agccttctat caaaaggcga
     5041 ccacagccat accagactag gtagaaagct gtaatttctc cctgtttaag gaattttggc
     5101 cgtctacgta acacacaaac caagccaaag cctagcaaat tccagagaga ctcaaacaag
     5161 aaggttggtt gacggtaggc accatcaata tacatctggt ctcggataaa ggctggcagg
     5221 tagttcaaac tttctactgc tttaccatag gcctcttggt taaagaaatt tccccaacga
     5281 ccgattgcct gagcaatcat gaccgatggc gctacaatat ccaaaaaatc cagagtatta
     5341 ataaagcgat actgggtaaa gaaataaagg ataatcgcac ctgtaatcaa accaccatag
     5401 atggcaatac caccgttcca gatagcgaag atagataaaa tgttatcctt atattcattc
     5461 catgaaaagg cgacgtagta aatccgtgcc cctatgatag aaagcggaaa tgcgattaga
     5521 ataaaatcta aaatatcatc ctgacgaatt ttctttcgcg gaccctcttt tgtcgccaga
     5581 taaacaccta gaatcagacc aagcaaaatg cagattgcat accaacgaat ttctaaggga
     5641 cctaatttaa ccgcaataga atccatatta agcctcttct ctattgtgtt caattagatt
     5701 agacaagcgt tcttcgaaaa tcttcgttgc atcaaacccc atctgtttgg cacggtagtt
     5761 catggctgct gcttcaatta cgacagaaat atttcgacca gtttttacag gaatacgtat
     5821 ttgtggaata gcaattcctg ctatttcgat agtatcgcca gagtttccca aacggtcaaa
     5881 taccttacct gtttcaaaat tttcaagata gacagctaat tgcacttctg atgaatcttt
     5941 aacggcacta gcaccgtata gactcatgat gtcgataatg ccgactcctc gaatctctaa
     6001 taagtggcgc agaatttccg caggttcacc ccagagagta acatcgtctt ttgcatagac
     6061 atcaacacgg tcgtcagcca ccaatcggtg tcctcgttta accaactcaa gaccagtctc
     6121 actcttaccg ataccagaat caccctggat aagcacaccc ataccgtaaa tgtccatcaa
     6181 gacaccgtgt acactcgtcc gctgagccag gcgactatcc aagtaagaag acaattctcc
     6241 cgatagacga ctggttgctg tcttgcttcg acagatagca atttgttttt cttttgcagc
     6301 cttgtacatc tcctcaggga tttccaagcc acgtgccact atgacaacag gggtttctgg
     6361 ctggaacatc tgagaaagaa cctgatagcg gttatgggca gtcatggcca tcaaataaga
     6421 ccactccttc atccctatca actggatgcg ctctggagca taataatcaa aatagccagt
     6481 catttccaat cctggtcggg taatatctgc cgtcgtaatt tgcttttgca ggagctcttc
     6541 tgtactgtaa gcacattcaa tccgcagatt atcgaccaaa tctttcacat aaacggtcat
     6601 ttcttccctc actttcagtt ttattatatt ataccataag ctaggggttg ggggaaaaac
     6661 aagtggctga tttggtaaaa agaatgagtt tctttctttt tctcacaaaa ttagcaaaac
     6721 ttccacagat taaatgcact tctcttccta cgctacatat ttttaacaaa tctgttatac
     6781 tttatataga tcaatactta ctactaagga gaaaatcatg gaacccttgt ttctcacacc
     6841 tgttatgcac gaaaaaattt gggggggcaa tcgcctaagg accaactacc actacgacat
     6901 tcctagtgat aagactgggg aatgttgggc tatttctgcc catccaaatg gcgtaacaac
     6961 tgtctcaaac ggtcaatata gaggaagagg actggatgac ctctataaga atgaaaaaca
     7021 tttatttggc aatccaacag atgatgtctt tccattatta acaaaaatcc ttgatgcaga
     7081 cgattggctt agcgtccaag tccatcccga tgatagctat ggcttggctc acgaaggcga
     7141 actgggcaag acagaatgtt ggtacatttt ggaagccgaa gacggagctg agatcattta
     7201 tggtcacaat gcccaatcca aagaagaact ccgtcagcaa atcgaagctg gagattggga
     7261 caagctgttg acccatgtac ccgtcaaaaa gggtgatttc ttctttgtac caagcggcac
     7321 catgcacgcc atcggtaagg gaatcctgat tttagaaact caacaatcta gtgatacaac
     7381 ctaccgtgtc tatgactttg accgccgtga cgatgcaggc aacctccgtg aactacacat
     7441 tgagaaatcg attgacgtgc tgaccatcgg acctgttgct aactccacac ctgcccacct
     7501 caaagcgggc aatcttgact cgacacttct ggttgccaat cccttcttta ccgtatataa
     7561 atggaatatc caacaagaaa tcaaaatgga acagacagtt ccttacctgc ttgtcagtgt
     7621 cattgaaggg gaaggtacca tccaagtcgg tgaaaccagc tacccacttc aaaaaggaag
     7681 ccactttatc ttaccagcca atgtgacaga ctggacattt acaggccaaa tgaacatcat
     7741 cgcaagccac acaaactaat actcttcgaa aatcaaaatt atccgttgtc aacttgcctt
     7801 gatgaactca agttctatct tcgacttcgt ttcctaggct acttttgatt ttcattgagt
     7861 ataagcatac aaaaaacttg agcctttaaa ctcaagtttt tttgcattat tcaaaccaag
     7921 acttatttat cttttccgct tcttttatct tacgtggacc tgttccatag cgttccgcgt
     7981 ccctatcttc cttataaggt aaaaaataag ctgctgcaat atagagagca atcagaagga
     8041 ggaaggattt gcctaagagg agacctgcaa taaatatcgc gcgtgttacc caggattccc
     8101 agcccaattt atgcgctatg ccggcaagaa ccccagatac tatcttcccc tgccttactt
     8161 tatacaattc aattgacatc ttttattcct ttcacgtaaa agcccccttg acctgtttgt
     8221 accttcaaga aaagtaaatc attgccagat ttttcaaact gatagggact atttttcttt
     8281 ttccggttaa ccaagacatg acctgattgc gactcaatct gcccccgaat tccctggcct
     8341 ttgccagtgt gtaaagtcaa gggagcctga gctagttgca catacaagtg ttggggataa
     8401 ccaccgcaag tcgtcaagcg aatatcctgt tgttttttat cccttgaact acggatagaa
     8461 atatcttcaa atggtaagta atccaagcga gcatctgcct gaccaagttc caaatccagc
     8521 accgacatcc attcctgagg caagttgatt gtcaaacgac aggccaagct actgtgaaaa
     8581 taaagcccct ttttttcaac ataaagacga ggaagcttat cttctggtgc atcttgattg
     8641 tagcaaatct ccacactaat ctgattattt ttagacttag ccaaagttag acgtccctca
     8701 gataatttta aaaagagtga ttcaatccct tcaaaactat aggattgttt caatttggag
     8761 gaagcaaaga ccttaaaact tggcaaggta aaattcaatt ttactttttc ttccacaaaa
     8821 agaaagtctg gttcttgatt catttcctta aaacaggcac gcgcctcttc cttggtcaag
     8881 acaccttttt catataactc aattaagcgt tcttctctct tttgcgtcat agactcacct
     8941 caattctact ctagtatatc acatttctcc acaaaaaaat cagcccgaag actgattcta
     9001 gaaaatcaag gtttaccaag gcaattgata gattttttct gaaaatggat gaaaattctg
     9061 ctttgaaacc ccaaaattta actctattat tcaactctga gcgtataaaa atcatcacgc
     9121 ttgggaactg cggaagttgg taaatgaagt attgtttatt ggtacaggta agagcaagtt
     9181 ttcagtttta gaatttggag agtttgaaat tttaggatct acttctgtta ttggatacga
     9241 taattcgtat gactatgagg gggattttat attgactgct cgtgttggtg cgaatgctgg
     9301 agagctttac agacattctg gaaaagtaaa aataagtgat aatacagtat ttattcaagg
     9361 agatttactt gatttcattt tctatattct acaacatttt gatattaaga aactatcatt
     9421 cggaacgggt cagccacttg tgaaagcaag cgaattaaaa ggattaaaga taattatgcc
     9481 tgtttctgta aaagaaagaa atcatgtcgg caccttcttc tccaccctgg accgccacat
     9541 cacccttcat cagcgtaagt cgatttgatg tttagcttaa gccagatagg taacgcatga
     9601 ctaaatctgt atcttgattt tcaagttccg agatgacatg catatagact ttttgggtgg
     9661 ttgtcatgga ggagtgtccc aatcgcctgg acacactagc tattgaaaca cctgcataca
     9721 tcaaaagaga ggcatgggta tgtctaagcc cgtggataga aatgactggt acacccacat
     9781 ttttgcagtg gcgctccaac cacttatttg gagtggagtt gcagactgaa ccattcacaa
     9841 aaataggctt atcctggggt aagtctttga tgagttgctg aaattgcata ctcaactgcc
     9901 aatcaatctg cactttccgc atggaagatt tatttttggt aggtaaaaaa ccaccatttc
     9961 ccttgtaatt ccatgtcttt gtcacggaaa cattctgctg aacaaagtta aaatcagctg
    10021 gtgtcagacc taaagcctct gaaaatcgca gccctgtctt cgcaatgagc aaaatcagcc
    10081 agtcccagtt gatccgacac tccaagtcca aatcagaaag aacattgtgg agttcaaact
    10141 gatttaagta tttcattttt ttaattcttg gtgcctttcc cttaaaaatc actttacggg
    10201 tcggatctcg gtcaatataa ccctcatcaa cagcatccaa gactgcaccc ttgagctgat
    10261 gatgaaagtc aatgactgtc tgacgttcat gggtcagagc atagtcattc aaaatttgct
    10321 gataggttgt cctgtccaac tgatgtaata ccaagtcagg cacaatattt tttaaatgat
    10381 aatgattatt gcgatacttt tcgagggtaa ttgggcgaac agctccctct ttaaacagtc
    10441 ttgtccattc tttaaaatat tcatgaagta aggtttctcg tgtaatttta ttcatagtat
    10501 tcattccttc tacgacttac gctgatgaag ggtgatttga cggtcgaggg tggagaagaa
    10561 agagccgatg gcggattgtt cggggtggga ggggagtttt atatcaaagt cactaagaac
    10621 ttccttggta attgttttga ttgttccacc gggggcacac tttgcttctt caataaattt
    10681 ttgtttaatt atataagccc aaaacaaatt atcaactggt aagttaaagt tttcaaaaat
    10741 cagtaccgtc ctatcaataa aagcctcata ttgggtaata gcaacacgac caatactacc
    10801 ttgtaaagta actagtacag aaccttcctc agcaaaaaca cttagtggtt gtgcttttct
    10861 actaattttt tgttttgtat ctcctactaa attcatttca tctgtgacat ctgcaacttg
    10921 aacaaaaggc attgctccgt caccgtcata ccactcacta tttccgtaag gttggggaaa
    10981 agaaccacgt ctaaatcttg ccactttccc taacttccgc agttcccaag caggagtatt
    11041 ttttacccta tcccacagtg gcggtggatg acattttatt catccgatag gtgctggaaa
    11101 atgttttctg ttggtttctg aaaacgacga aaaagaccaa gaaaaatctt ggccttagtg
    11161 gtcttatttg tatttttgct tggtttctgc catccattgt ggtagaacct tgttcaaatt
    11221 catattgagt tttagcttgt tgccagcgaa ctcattggtg gcttgatggg ggtctaaggt
    11281 cgtagcgatt tcctcacggt aaggaatatc atctggctta cttgtgttgt attcctgtag
    11341 tgactttcta agcaagtcac tatcaattcc ccactgacca gcaaattcct tgatgaccgc
    11401 aaactgtcta ttctcctgcc attctttaaa agcgacgtca aaatcgacag aggcagacaa
    11461 ttgacctgtg accaattctt cgttaataaa ggttttcaag gcttcagcca gttcattctc
    11521 accaaggtca ctcaattctt ggatttgctc caaaatcaag accaaattat tgtcattgat
    11581 tacttggaaa ccaccttcat tggttgtttt gtttcctatc aaagcaaata aactttgggc
    11641 atcaatgatt tgactaccct gcaatttggt acgtcccttc aagtcttttg gtacccaatc
    11701 gtcaccacca gttcctctag gaaacaggtt tctataagca ccaatgtact ggagccagct
    11761 atgctcatcc aaaccaaagg cttcatcatc ccagacaaat tcatagtatt gcttgacacg
    11821 atccagttgc ttgaaggttt ccttaaaagc agttttaaac gttccctttt tctcctcatc
    11881 ttgctggatg tcaatccact gggtcgggtt gggcaattct gaccgtagag cctctacttt
    11941 tgtccgaagt aagtctaccg caaccccata tttttcaaca agaacacggc tattgcttcc
    12001 accactaccg tatagcaaca aggcttcctt aacaatttcc tctgttatgc gagggtattg
    12061 gaaattgata atggtaccaa attccttgct ggaaccaaaa acccggttgg ttcgcgaata
    12121 tgcctgtatt aagccctgta attccagctt cctatcaacg tagagagtat tcaagcgttt
    12181 ggcatcataa cccgtcaaaa gctgatctgc tacgatgaga atatcgatat tcttaggatt
    12241 acgaccactt ccaccacgct tggcacggtc agtcacatct tcaaaataag catcctcacc
    12301 ctttttctta tcaccagcaa taaaacggat acctgtaaaa ccttcataat ccgcgaacat
    12361 ctcttcaatg acttccctag cgacattgtc aggatcattt tcatttccaa aactaaaggt
    12421 catggcaata ttgggcttta cagctccatc cgtaaactgc tctgctatct gctttttaaa
    12481 ctcgtgatag taggcgatga ctcgctcctt aaaagcaacc gtcagcatgg cattgaactc
    12541 accaccctgt gattgttcct gccagcccga caaaatctcc tctaccactc tagggatgtg
    12601 ggtttgattg tgatagacca aaagtccctc tttggccgca tgcttctcta catccaattc
    12661 tgacagccgc atgacttgac gttcaatctc tttgacagct ttttctggat gtttttctgt
    12721 tagaagctca atcaaatcct cacgtaaatc ttcataactc ctaaattccc ctgtattgat
    12781 gtagtccaca tggaagccaa ggacattccc atccgcgatc gcttcgtcaa tggtatattg
    12841 gtgaaggagt ggaccaaaca atttttcagt cgtgttaatc agctcactgc gttcgttaat
    12901 tttccccttg accttgtttt catcaaagag gggagtacct gtaaaaccaa agaatagacc
    12961 atttttatag aaataatcct taatcatccc catcatatca cccatggttg tcctatgggc
    13021 ctcatcaatg atgaaaatga ttttcttatc tgccaaagtc ctatcatcac catctatcaa
    13081 gtccttgacc aagttactca atttgaaggt ggtggtcaca acaatacctt tatgggagtc
    13141 actaagagtt ttacgtagct gataagcgct ttttgtttcg tccaccgtaa caaaatcgta
    13201 agtcgcataa gctttgaaag cttttgcggt ctgagcatct aaatccctcc tatctagcaa
    13261 gaaaactatc tttgctgcat cagttttact tgacaacaac atagccgttt taaagctggt
    13321 aatggtcttt ccagaacctg tcgtatgcca aacaaagcca ccatggggta ttttttccgc
    13381 attatcccat ccaaaagctg ccatttctac agcttgaact gcatagacct gataaggtcg
    13441 cataagcatg tggcgtctat tctcctcatt ttcagtatct tcgttaataa tgagatagtc
    13501 gccaaccatc tgatgagcca tgggaaccat cagaaaatgc tcaatgactt ctctgtaatc
    13561 tgtcaagacc ctttgtccca aatgcttacc acttgcttga tctttcttgg tgacagacca
    13621 atgaaaaaca aaggcaggat taaaatcatt gatagactta ggcgtcgcaa aataccgggt
    13681 ttcgatgtct gttgtgacca ccatcatttg agaaaaggac atgaagttat tggtgtattc
    13741 cccatcggca tagtagcgtt taaactgacc aaaggcctcg tccaaggact tgtcactccg
    13801 cttttgctca atgttaatca agggcagacc gttaatcaat aagactaggt caaaacggtt
    13861 gccattgtcc gaccaaactt cgcgagccac ttggtagctg gaatctcctc cactaacctg
    13921 agcctttttc aaaatggtta aggtaatttg ctttttggtc acatgtgggt gctggtcacg
    13981 gtaaatacca tcgattttgc cagtagaacc ttccatggat aggatttttg cggcctcgta
    14041 gctattggaa atctgcttga cctttgtcat aacttgatcg aactcgctat cggtaagagc
    14101 aacaccttcc aagacatccg cattcatacg atttagctct gaccgccaat tatttatcaa
    14161 atcctgaact gtaactttct gtaagtttcc atttaggtgt tcaggagttt gccatttaaa
    14221 cttggataat tcccgaacga atttatcttg aaattcacgt tctgaagcgt tttttgagat
    14281 agtttccata catttccttt ctatcatcat gtaagtaact gctacttacg ctgaaaatca
    14341 tacaaacagc tcatttaaaa gtgctttttt caagtttttg agtttttcta gcttacgctg
    14401 atgaagggtg atg
//
LOCUS       DATUCG010000056        14296 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.057, whole genome shotgun sequence.
ACCESSION   DATUCG010000056 DATUCG010000000
VERSION     DATUCG010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14296
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.057"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            143..364
                     /locus_tag="VCA20_001329"
     CDS             143..364
                     /locus_tag="VCA20_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003077462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEP1835143.1"
                     /translation="MTRDEVVDKPSQLNYLGLIHLAFSLGSEEAVDELTERLVAAGYL
                     LLSGPRITGDGYYESCELGFDDIQIELTV"
     gene            658..2091
                     /locus_tag="VCA20_001330"
     CDS             658..2091
                     /locus_tag="VCA20_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEP1835144.1"
                     /translation="MSKLPKNFLWGGAVAAHQLEGGWQEGGKGISVADVMTAGRHGVP
                     REITAGVLEGKYYPNHEAIDFYHRYKEDIALFAEMGFKCFRTSIAWTRIFPKGDELEP
                     NEAGLQFYDNLFDECLKYGIEPVVTLSHFELPYHLVTEYGGFRNRQAINFFVRFAETC
                     FRRYKDKVKYWMTFNEINNQANYQEDFAPFTNSGIVYQEGENREAVMYQAAHYELVAS
                     ARAVKIGHEINPDFQIGCMIAMCPIYPATCKPSDILMAQKAMQKRYYFADVHVHGFYP
                     EHILRYWERKGISVDFTEEDREDLLEGTVDYIGFSYYMSFAIDSHRENNPHYDYLETE
                     DLVKNTYVKASDWEWQIDPEGLRYALNWLTDMYHLPLFIVENGFGAIDQVEEDGMVHD
                     DYRIDYLGAHIKEFIKAVDEDGVNLMGYTPWGCIDLVSAGTGEMRKRYGFIYVDKDDE
                     GNGSYARSPKRSFAWYKNVITTNGEEV"
     gene            2173..2412
                     /locus_tag="VCA20_001331"
     CDS             2173..2412
                     /locus_tag="VCA20_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET family sugar transporter"
                     /protein_id="HEP1835145.1"
                     /translation="MVGFIAACLTTFGFVPQVIKVVKTKDTESISLGMYMMSVAGMSL
                     WLFHGIIQGDTALIIANSLSVTLAGIILVYKLVYK"
     gene            2635..2973
                     /locus_tag="VCA20_001332"
     CDS             2635..2973
                     /locus_tag="VCA20_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835146.1"
                     /translation="MESIVGIVVIVVVTVVLVYLLTNMWKIRAASVGQTDDTTLYFVR
                     FKGVNPPDLANSSVFNQVAKIKCAKGDTYQVTSTVNDVKLRDVIMDEFQLKSSEVVVY
                     YPPRLFFYAS"
     gene            3150..3464
                     /locus_tag="VCA20_001333"
     CDS             3150..3464
                     /locus_tag="VCA20_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003037418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HEP1835147.1"
                     /translation="MVKALIICAGGMSSSLIAKKTQTLLEEQGHKVEMNAVGVPEGGK
                     KINAAEYDLYLISPQTKMHFKQFEEAGKKVGKPIVQIPPQAYIPIPMGIEKMAKLVLD
                     NI"
     gene            3511..5484
                     /locus_tag="VCA20_001334"
     CDS             3511..5484
                     /locus_tag="VCA20_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEP1835148.1"
                     /translation="MLSTKEKMILQYLYQHQNVFSTSKVLAEHLSYTDRTIRTYIKKM
                     ASEISEGETGFAILSKQGYGYQLRISDEEKYHRFLSENQLIFGVDYSDAENRYKQILS
                     KLIFEEECILFDDLADQLFVSRSTLSHDFKHIREQLASYGLSIESKPDKGVYVVGEER
                     QKRHFIMDYFFSEHFYQNIHHFLSIDKSLALPLSFEELTIVVLDECRDSQLKLSDYII
                     QNLVIHLGLAIQRYQKGFTISPVTIDKEKYAEELAVATKIARRLSHRLEPQNRFPEEE
                     VVYIALHLISKTQLEVEKSSDRLLLRQALFEVLGRYDDEYGYQFSHDFSLIEGLLTHL
                     EVLVERLRHNLHIDNPLLDDIKTTYHDIFDLTQSLMAELSVFHSYSLSESEIAYVALH
                     LMASLERQKEKYKLNVLVICATGYGSAQMLKNRIKNELGQQVSISDVIGYYDLDNQKL
                     QGIDVIMSTIDLSGLVFKVPVITVSVFLTDQEVKEVKQRLADLRPSLQKPQVVERESN
                     LEKYFDQYFSEQRFLVVESAEKEMLLKQMISQLESEGLGNHQEFYKLIEARESLSTLV
                     FDKDIAVPHPLKPISEKHMISVAVIKNGLYWEKGYGQVRLVFLVSPSIYTNEGLSTIT
                     NRLVDLVDLPEVKKDIINSPDFETFKHVFLQRE"
     gene            5495..5809
                     /locus_tag="VCA20_001335"
     CDS             5495..5809
                     /locus_tag="VCA20_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HEP1835149.1"
                     /translation="MNTEELQMAAFGIILSSGNARSVIHEAFAAMREGDYDKAEELLE
                     AANADMLEAHHSQTGLLQEYASGTEVKVEIIMVHAQDHLMTTMTLREVALEMLALYRK
                     VG"
     gene            5824..6336
                     /locus_tag="VCA20_001336"
     CDS             5824..6336
                     /locus_tag="VCA20_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835150.1"
                     /translation="MSKGMASKKLQKTPEQLKQESKRKSQLFNRYMLFRYSLAVFFFA
                     NLYWLLVQLFQPSLYLILPSLMLIAIIIATAEQFKLYSAEESRLTKTEFVLRAQAILQ
                     GLIVVLVILGHISTLFPTFSNHTSAKLFVVALQLLGLALVVLNIKRLEQVRQNTDKYY
                     LRFQIIRKYL"
     gene            6356..7705
                     /gene="celB"
                     /locus_tag="VCA20_001337"
     CDS             6356..7705
                     /gene="celB"
                     /locus_tag="VCA20_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEP1835151.1"
                     /translation="MEEQKGLFGLLDKYLMGPMGKLASYKVVRAITAAGMAAVPFTIV
                     GSMFLVFSILPQAFSFWPIVSDIFAASFDKFTPLYMVANTATMGVLSLYFALAIGYEL
                     TKIYAEEEELNLTPLNGALLSLFAFIMTVPQIIFGNGAMSAVTSLKEGAVVVDGWAMG
                     GGVTRFGTTGIFTAIIMAVAAVLLYRMCVKNNWVIKMPESVPEGVSRGFTALIPSFVV
                     AFFVIFLNGVLVALGTDIFKVIAIPFSFVGNLTNTWIGLVIIYLLTQALWVVGIHGAN
                     IIFAFVSPIALANMSLNAAGERMIVAGEFSNMFVIAGGSGATLGLCIWLATRARSEQL
                     SAIGKAAIVPGIFNINEPLIFGLPIIYNPALAIPFMLAPIASMTVYYFSMKLNLINAV
                     VAQVPWPTPVGIGAFLGTADWRAIVVSIVCAVVAFLVYYPFIRAYDKQLLKEEVANA"
     gene            8114..9586
                     /gene="ascB"
                     /locus_tag="VCA20_001338"
     CDS             8114..9586
                     /gene="ascB"
                     /locus_tag="VCA20_001338"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004231462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEP1835152.1"
                     /translation="MTKQFPKGFLWGGATAANQYEGGWNLGGRGPATSDTYIAVDPDK
                     RKDMSHFGKPVSRADVEFALADQEGLYPKRWGSDFYHRYKEDIALFAEMGFKTFRLSI
                     AWSRIFPKGDELEPNEEGLAFYDAVFDELNKYGIEPLVTLSYYEFPVHLVTEYGGWKN
                     RKVIDFFVRYAETVFNRYKGKVKYWLTFNEINILGMTGYLSGGLLFEDGQLHLDEMYQ
                     AAHHQFVASSLATKVAHEIDPENKVGCMLARMEAYPATCHPDDMMHALQKDQSNLFYS
                     DVQVRGEYPRYMNRFFKENNITIYFEPGDEAILKQYPVDFMSFSYYMTSVTRHPNNQT
                     PEQKATAGNLILGEANPYLEASDWGWQIDPVGLRLTLNKLYDRYQVPLFIVENGLGAL
                     DTVEKDGSIHDNYRIDYLCKHVEQMYEAIEDGVELMGYTWWGCTDLISASTSEMSKRY
                     GFVYVDADDQGNGSFDRSRKDSFFWYKDLIVSNGASILND"
     gene            9595..10194
                     /locus_tag="VCA20_001339"
     CDS             9595..10194
                     /locus_tag="VCA20_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019805915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEP1835153.1"
                     /translation="MVKTIYLMRHGETLFNTQGRVQGVCDSPLTENGRAQAQAAKNYF
                     AEQGVQFGAVYSSTQERATDTAKIVSGQENVQQLKGLKEMDFGIFEAQPEMLLPKFRP
                     GANSFEDLLVPFGGEDIRSVGQRVAQTVEDTVSEQEENTVLAVSHGAAMWGFVLHHKI
                     ELPADFRFSNCAICQLEYDGDQIRLVKVINPVLNQVIDL"
     gene            10206..10808
                     /locus_tag="VCA20_001340"
     CDS             10206..10808
                     /locus_tag="VCA20_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEP1835154.1"
                     /translation="MARKLYLMRHGQTRFNQQGRIQGACDSPLTELGREQALAAHQYF
                     QEQGIEFDKIYSSTQERACDTAELVTGRTDYTRLKGLKEQDFGAFEGQQEYLNPPLQG
                     DIGYGNYFVTFGGESYQDVRQRMVETIGGIMEEADNQSVLVVSHGAAIAQFFRQVLTD
                     YPQVRMRNCAILTFDYEDGKYDLLSVVDPVNREILYQQQS"
     gene            10823..12262
                     /locus_tag="VCA20_001341"
     CDS             10823..12262
                     /locus_tag="VCA20_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEP1835155.1"
                     /translation="MTVTKVFPEGFLWGGATAANQCEGAYDVDGRGLANVDVVPIGED
                     RGAIITGRKKMFDFEDGYFYPAKESIDMYHHFKEDIALFGEMGFKTYRLSIAWSRIFP
                     KGDELEPNEKGLQFYEDLFKECHKYGIEPLVTITHFDCPMHLIETYGGWRNRKMLEFY
                     ERLCRTLFTRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENVEQIKYQAAHHELV
                     ASAIATKLAHEIDPENKVGCMLAAGQYYPHTCHPRDVWEGLQEDRENYFFIDVQARGY
                     YPNYAKKKWEREGIEIEMTDQDLALLKEHTVDFISFSYYSSRVASGDPAEKEKTAGNI
                     FASIKNPYLDASEWGWQIDPLGFRITLNSIWDRYQKPLFVVENGLGAVDTPDEKGYVE
                     DDYRIDYLRQHILAMRDAIVEDGVELLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDN
                     EGTGTLKRSKKKSFEWYKKVIATNGADVG"
     gene            12346..13335
                     /locus_tag="VCA20_001342"
     CDS             12346..13335
                     /locus_tag="VCA20_001342"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014677692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEP1835156.1"
                     /translation="MKYIVNNSNDPAYNIALEAYAFKELTDIDEIFILWINEPAIIIG
                     KHQNAIEEINKEFTDEKGIHVVRRLSGGGAVYHDLNNLNYTIISNKADEGAFDFKTFS
                     KPVIDTLATLGVEANFTGRNDLEIDGKKICGNAQAYAKGRMMHHGCLLFDVDMSVLAS
                     ALKVSKDKIESKGVKSVRARVTNINNELPEKMTVLEFKDAILNQMKLEYPDMDEYVLS
                     EGDLARIQEIRDTQFATWDWTYGQTPEYTVERSVRYPAGKITTYIKAEKSVIESIKIY
                     GDFFGIGDVSDIEDLLVGTRYEYADVLAKLQEIDTTHYFSRMTTEEVAKAIVA"
     gene            13507..13797
                     /locus_tag="VCA20_001343"
     CDS             13507..13797
                     /locus_tag="VCA20_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEP1835157.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATIVESKAWE
                     KRRLAYEIKDFREGLYHIVNVEANNDEALKEFDRLSKINGDILRHMIVKLDA"
     gene            13809..>14296
                     /locus_tag="VCA20_001344"
     CDS             13809..>14296
                     /locus_tag="VCA20_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEP1835158.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNQAVATFTLAVNRNFKNQNGER
                     EADFINVVIWRQQAENLANWAKKGALIGVTGRIQTRSYDNQQGQRVYVTEVVAESFQL
                     LESRTAREGQGGGYSAGNSFAGGNNYNSPYQAPAQSTPNFAREESPFGASNPMDISDD
                     DLP"
BASE COUNT         4248 a         2606 c         3204 g         4238 t
ORIGIN      
        1 ctatacactc aggatttgga aggtatgcgt aatttctttg aaaactactt caatgccaca
       61 tccaatcagc tctaccataa tcttaaaacc agtttcaagt cctatttcct tacctttgaa
      121 gacgggggct cgtttggaaa ttatgaccag agacgaagtg gtcgacaaac caagtcaact
      181 caattatcta ggactcattc atttagcctt tagtctaggg agtgaggaag ctgtggatga
      241 gttaacagag cgacttgttg ctgcaggcta cttgcttctt agtgggccac gaataactgg
      301 ggatggctac tatgaatcgt gtgagttggg atttgatgat atacaaattg aattaacagt
      361 gtaatgttgg caagtaatac tgctagatca atgtttaacg gattagcttg caagtctcat
      421 tcattagcga tttccttact gttacggaaa aaactgcaat ggtgattgat gtaaacattt
      481 gctaaactaa taactatagg aacctgtatt cacaagtagg gtgcttgtat ataagagatg
      541 tttttgaggg aatactgaca gtattttgaa aaaactaaca atgagtagct taaaaattag
      601 tcttagatgg aagttctgaa ctgtaaatac aggttctaaa atatacagga ggcaattatg
      661 tctaaattac ctaaaaattt tttatggggt ggagcagtag cagctcacca acttgaaggt
      721 ggctggcaag aaggtggaaa agggatttct gtagcagatg tgatgactgc tgggagacac
      781 ggggtaccta gagaaattac agctggcgta ttagaaggaa aatattatcc gaatcatgaa
      841 gcgattgatt tctatcatcg ttacaaagag gatattgctc tctttgctga gatggggttc
      901 aagtgtttcc gtacctcaat tgcatggaca cgtatttttc caaaaggaga cgaattagag
      961 ccaaatgaag ctggtttgca gttctatgat aacttattcg atgagtgttt aaaatatggg
     1021 attgaacctg ttgttactct atcacacttt gagttgcctt atcatctggt aaccgagtat
     1081 ggtggtttta gaaaccgtca ggcaattaat ttctttgtcc gttttgctga gacctgcttc
     1141 cgtcgttaca aggataaggt caaatactgg atgaccttta atgagattaa taatcaagca
     1201 aactatcagg aagattttgc accgtttact aactcaggta tcgtttatca agaaggtgaa
     1261 aatcgtgaag cagttatgta tcaagcggcc cattatgaat tagttgcctc tgcacgcgct
     1321 gttaaaattg gacatgaaat taatcctgac tttcaaattg gttgtatgat tgccatgtgt
     1381 ccgatatatc ctgcaacctg taaaccatca gatattttga tggcacaaaa ggcgatgcaa
     1441 aaacggtatt actttgcaga tgttcatgtg catggtttct accccgagca tatcttgcgc
     1501 tactgggaga gaaaaggtat tagcgttgat ttcacagagg aagatcgcga agatttgcta
     1561 gaaggaacag ttgattacat cggtttctca tactatatga gttttgctat tgattctcac
     1621 cgcgagaata atcctcatta cgattatctt gagacagagg acttggtgaa aaatacctat
     1681 gtgaaggctt ctgattggga atggcaaatt gacccagaag gattgcgcta tgcattaaac
     1741 tggcttacag atatgtatca tctgccactt tttatcgtag agaatggctt tggtgcgatt
     1801 gaccaagtcg aagaagatgg aatggtgcat gatgactacc gtattgatta tcttggtgct
     1861 catatcaaag agtttatcaa ggctgtcgat gaggatggag ttaacttgat gggatatacg
     1921 ccgtggggct gtattgacct ggtgtcagca gggacaggtg aaatgcgcaa acgctacggt
     1981 tttatctatg ttgataaaga tgatgaagga aatggtagtt atgctcgttc tccaaaacgc
     2041 tcattcgcat ggtacaaaaa tgtcatcaca acaaatggtg aggaagttta atgagaagac
     2101 ctctgtagcg taatagctac atgggtcttc atttatgttt tttagtattg gtatattaaa
     2161 ggagaaaatt gtatggttgg ttttatcgca gcttgcttaa ctacctttgg atttgtacct
     2221 caggttatca aggtagttaa gacaaaggat acagagtcga tttcgttagg aatgtatatg
     2281 atgtctgtgg cagggatgtc cttatggttg ttccatggta ttattcaagg agatacggcc
     2341 ctcattattg ctaatagcct atctgtaaca ttagcaggta ttattttggt ctataaatta
     2401 gtctacaaat aatgcactat tccatcgata aaagaatttt tagcaacagt gaaaaacatt
     2461 gaaaatgctt gactttttct gtgaaaggaa tataatgatg acaaataaat attggaagtg
     2521 tgattactat gttattcgtt ccgctttttt taaggtttaa cctttttaaa ccaccaagat
     2581 aagcgttttt tctagttgtt tgtaaatgta tgtatcttgg aaaagaggtt tattatggaa
     2641 tctatagttg gaatagttgt cattgtagtt gttacggttg ttctggttta tttattaact
     2701 aatatgtgga aaatcagagc ggcttcagtt ggtcaaacgg atgacactac gctatacttt
     2761 gtaagattta agggggtaaa tccgccagac ttagcaaata gtagtgtttt taatcaagta
     2821 gccaaaatca aatgtgctaa aggagatacc tatcaagtca cttcaacagt caatgatgtg
     2881 aaactgagag acgtgattat ggatgaattt cagctcaaat cttctgaggt tgtagtttac
     2941 tatcctcctc gtcttttctt ttatgcatca tagtcgcaac tcttcggagt tgctattttt
     3001 ttgtttctgg agagtagcgg aaaagcggtt tattttagta gcgaaatttc cgtagtggta
     3061 aggaaatact tcctatggaa aatgcaagcg tgttcatgta aaataaaatc ataacaaaag
     3121 taaataataa aatagttggg ggaaatccta tggtaaaagc tttgattatc tgtgcaggag
     3181 gcatgtcttc ttcacttatt gctaaaaaga cacaaacact tttggaagaa caaggtcata
     3241 aagtagaaat gaacgctgtt ggtgttccag aaggtggtaa aaaaattaat gcggcagagt
     3301 atgacttgta cttaattagc ccgcagacaa aaatgcactt taagcaattt gaagaagcag
     3361 gtaagaaggt tgggaagcca attgtccaaa tcccaccaca agcttatatc ccaattccga
     3421 tgggaattga aaaaatggct aaattggtct tggataatat ctagttcttt gctaccatag
     3481 atggtatatt gattcgaaaa gagggtagtt atgctaagta ctaaggaaaa aatgatattg
     3541 cagtatcttt accaacacca gaatgttttt tcaaccagta aagtattggc agagcatctc
     3601 tcttatacag atcgcacaat tcgaacttat ataaaaaaaa tggcatccga aatatctgag
     3661 ggagaaacag gttttgcgat tttatccaaa caaggttatg gctatcagct tcgaattagc
     3721 gatgaggaaa aataccatcg tttcttatct gaaaaccaac taatattcgg ggtggattat
     3781 agtgatgcag aaaatcgcta taaacaaatc ctgagtaaat tgatatttga agaagaatgt
     3841 atcttatttg atgatttggc ggaccagctg tttgtcagta gatcaacctt gtctcatgat
     3901 ttcaagcaca ttagggagca acttgcttct tatggtttga gcatcgagag taagcccgat
     3961 aagggagtct atgtggttgg tgaagaacgt caaaaacgtc acttcatcat ggactatttc
     4021 tttagtgaac atttttatca gaatatccat cattttttat ccattgataa aagtctagcc
     4081 ttacctctat cttttgaaga gctgacaatt gtagtcttag atgagtgtcg tgatagtcag
     4141 ttgaaactat ctgattatat tatccaaaat ttagttattc atttaggttt agctatccaa
     4201 cgctatcaaa aaggttttac aatttctcct gtcaccatcg ataaggaaaa gtatgctgaa
     4261 gaattggcag tagctacaaa gattgctagg cgattatccc atcgactgga acctcagaat
     4321 aggtttcctg aggaagaagt ggtctacatt gctctacact tgatttcaaa aacacaattg
     4381 gaggtggaga agtcatcgga ccgcctcttg ttgcgccaag cactttttga agttttaggg
     4441 cgttatgatg atgaatatgg ttatcaattt tcacatgatt tttccttgat agaaggtttg
     4501 ctaacacatc tggaggttct cgtagaacga ctccgtcaca atctccatat tgataacccc
     4561 ctacttgatg atattaaaac aacttatcat gatatcttcg atttgacaca atcactaatg
     4621 gctgaacttt ccgtttttca ctcttatagc ttgtccgagt cagaaattgc ctatgtagct
     4681 ctacacttga tggcttcctt ggaacggcaa aaagaaaaat acaaactaaa tgttctggtt
     4741 atctgtgcaa caggctacgg tagtgctcag atgctgaaaa ataggattaa aaatgaactt
     4801 gggcaacagg tgtccatatc agatgtcatc ggttactatg atttagataa tcaaaagttg
     4861 caggggatag atgtgataat gtcaaccatc gacttatctg gtttggtgtt taaggtccct
     4921 gttataactg tcagtgtctt tttaacggat caggaagtta aggaagttaa acaacgattg
     4981 gcggacttga gacctagtct ccaaaaacca caggtagtgg aaagagaaag taacttggaa
     5041 aaatactttg atcagtactt ctcagaacaa cgatttttag ttgttgagtc agctgaaaaa
     5101 gaaatgttat taaagcaaat gatttcacag ttggagagcg aaggtctagg aaatcaccag
     5161 gaattttata aattaattga ggcgcgtgaa agcttgagta cattggtatt tgataaagat
     5221 atagctgttc ctcaccccct aaaacccatt agcgaaaagc acatgatttc tgttgcggtt
     5281 ataaaaaatg gtctatattg ggaaaaaggc tatgggcagg ttagattggt tttcttagtg
     5341 agtccatcta tatatacaaa tgaaggtcta tcaaccatta ccaatcgatt ggtagatttg
     5401 gtagatttac cagaagtcaa gaaagacata ataaactctc ctgattttga aacattcaaa
     5461 cacgtttttt tacaaagaga atagaaggtg acatatgaat acagaagaat tacaaatggc
     5521 tgcatttggg atcatcctca gtagtggcaa tgcaagaagt gttatccacg aagcttttgc
     5581 tgctatgaga gaaggtgatt atgataaggc agaagagtta ttggaagcag ccaatgctga
     5641 catgcttgaa gcccaccatt ctcaaacggg tctcctacag gaatacgcaa gcggtactga
     5701 agtgaaggta gagattatta tggttcatgc gcaagatcat ttgatgacaa caatgacttt
     5761 acgtgaggta gcgcttgaaa tgttagcttt gtatcgtaaa gttggttaga aagaggatat
     5821 gggatgagta agggaatggc aagtaaaaag ctacagaaaa caccagaaca attaaaacaa
     5881 gagtctaaac gcaaaagtca gctctttaat cgctatatgc tgtttcgtta tagtttagca
     5941 gtgttcttct ttgcaaatct atactggctt ctggttcaat tatttcaacc aagtttgtat
     6001 ctaatcctgc cctcgcttat gctgattgca ataatcattg cgacggcaga acaatttaag
     6061 ttgtatagtg ctgaggagtc tcgattgaca aagacagaat ttgtattaag ggcacaagca
     6121 atcttgcaag ggttaattgt ggttttagtg attttgggtc atatctcaac cctctttcca
     6181 actttttcaa accatacatc tgctaaactc tttgttgttg ccttgcagtt gttaggtctt
     6241 gctttagttg ttctcaatat aaaaaggttg gagcaggtaa ggcaaaatac ggataaatac
     6301 tacctacgtt ttcaaataat aagaaaatat ctttaaaatt ttaggaggtc ttctcatgga
     6361 agaacaaaaa gggttatttg gactattaga taaatatctg atgggaccaa tgggtaaact
     6421 ggcatcttac aaagttgtac gtgccatcac agctgcaggt atggctgccg taccatttac
     6481 gattgttggt tcgatgttct tggttttcag tatcttacca caggcatttt cattctggcc
     6541 aatcgtttca gatatttttg cggcatcttt tgataaattt accccactct atatggttgc
     6601 caatactgct actatggggg tgctatcact ttattttgca cttgctattg gatatgaatt
     6661 gacaaaaatt tatgctgagg aagaagagtt aaatctaaca cctttgaacg gtgccctttt
     6721 atcacttttc gctttcataa tgactgtgcc acaaattatc tttggaaatg gtgctatgtc
     6781 agcagttact tcacttaaag aaggtgcagt agttgtagat ggttgggcaa tgggtggtgg
     6841 cgtaacacgc tttgggacaa caggtatctt cactgctatc ataatggcgg ttgcagcagt
     6901 tcttttgtac cgtatgtgtg taaaaaataa ctgggtaatt aaaatgccag aatctgttcc
     6961 tgagggtgtg tctcgtggtt tcactgcttt gattccttca tttgtcgtag ccttctttgt
     7021 aattttcttg aacggtgtat tggttgcgct tggtactgat atatttaaag ttattgcaat
     7081 tccatttagc tttgttggaa atttaacaaa tacttggatt ggtttagtta ttatctactt
     7141 gttgactcag gcactttggg tagtaggtat tcacggtgca aatatcatct ttgcttttgt
     7201 tagtccaatt gcacttgcaa atatgtctct taatgcagct ggtgaacgaa tgattgttgc
     7261 aggtgaattt tcaaacatgt tcgtcattgc aggtggttct ggtgcaaccc ttggactatg
     7321 tatttggtta gctactcgtg ctcgttctga acaattgagt gcgattggta aagcagcaat
     7381 tgttccgggt attttcaaca tcaatgaacc actgattttt ggtttgccaa tcatctataa
     7441 tccagcactt gcaatcccat tcatgcttgc tccaattgca tctatgacgg tgtattactt
     7501 ctctatgaag ttgaatctta tcaatgcagt tgttgctcaa gtaccatggc caactccagt
     7561 tggtattggt gccttccttg gtacagctga ctggcgtgca attgttgttt caattgtttg
     7621 tgcagttgtt gccttccttg tttactatcc attcattcgt gcatacgata aacagttgct
     7681 aaaagaagaa gtggcaaatg cttaaactag agaaactcct tgacttcttc taagtctaag
     7741 gagtttttct ataagagaat atcaggagac attgttagat aaacataaga tagtatcata
     7801 tttgctttct tctttccaaa tcagaggagc tgaggtagag caagagaaga agatttttat
     7861 agctgacttg atgaagcgac tatcaataca tatggaggca ggcttaaaaa tatctctact
     7921 gcttttgcta atactaattg agagaaaacc tctcccttgt tccaagtttt aattgggata
     7981 ttatctaatg ttttaagaat agtgataatt ggaaatagca tagaatagta tcagtttggc
     8041 atattatcac gtaggaatga agggagttct tttgctatta tatagctaac aaagccagaa
     8101 ggagtattgt gctatgacaa aacaatttcc aaaaggattt ctttggggcg gtgcgacagc
     8161 ggccaaccaa tatgaaggtg gttggaattt gggaggtcgt ggtccagcca cttccgatac
     8221 ctatatagcg gtcgatccag ataagcggaa ggatatgagt cactttggta agcctgtttc
     8281 gcgggctgat gtggaatttg ccttggcaga tcaagaaggg ctctatccta aacgctgggg
     8341 gtcagatttc tatcatcgct acaaggaaga catcgccctc tttgcagaaa tgggcttcaa
     8401 gaccttccgc ttgtcgattg cctggtctcg tatttttcca aaaggagacg agttagaacc
     8461 gaatgaagaa ggactggcct tttatgatgc ggtcttcgat gagctgaata aatatggtat
     8521 cgagccactg gtgacccttt cgtattatga attcccagtt catctggtga cggaatatgg
     8581 tggttggaag aaccgcaaag tgattgactt tttcgttcgc tatgcagaaa cagttttcaa
     8641 tcgttacaag ggcaaggtca agtattggct aaccttcaat gaaatcaata tccttggtat
     8701 gactggttat ttatcaggcg gcttgctatt tgaagatggt cagttgcact tggacgaaat
     8761 gtaccaagca gcccaccatc agtttgtcgc ttctagttta gcaacaaaag ttgctcatga
     8821 gattgaccca gaaaacaagg ttggttgtat gctggcacgg atggaagcct atccagcaac
     8881 ctgtcatcca gatgatatga tgcacgcgct acaaaaagac caatctaacc tgttttactc
     8941 ggatgttcaa gtgcgtgggg agtatccgcg ttacatgaat cgcttcttca aggaaaataa
     9001 tatcaccatt tactttgaac caggcgatga agcgattttg aaacaatatc ccgtggattt
     9061 tatgagtttt tcatactata tgacctctgt tacccgccat ccaaataatc aaacgccaga
     9121 gcaaaaagcg acagcaggaa acttgattct aggcgaagca aatccatatc tggaagcctc
     9181 cgactggggt tggcagattg atcctgtcgg cctccgtctc acgctcaata agctctacga
     9241 ccgttaccaa gttcctctct tcattgtgga aaatggctta ggtgccttgg atacggttga
     9301 gaaggatggt tcaattcacg ataattatcg gattgattac ctttgtaaac atgtggaaca
     9361 aatgtatgaa gccatcgaag atggggtaga actcatgggt tacacctggt ggggttgcac
     9421 ggacttgatt tcagcttcaa catcagaaat gtccaaacga tacggttttg tttatgtaga
     9481 tgcggatgac caaggaaatg gcagctttga ccgttcacgg aaggattcct tcttctggta
     9541 taaggacctt atcgtaagta atggagcaag cattttgaat gactaggagg ttctatggta
     9601 aaaacaatct atttgatgcg ccatggggaa acacttttta acactcaagg tcgtgtacag
     9661 ggcgtttgcg attctccttt gacggaaaat ggtagagcgc aagcacaggc ggctaagaat
     9721 tactttgctg agcaaggtgt ccagtttggt gcagtctatt cttccacaca ggaacgtgcg
     9781 acagatactg ccaagattgt gtcaggtcag gagaatgtcc agcaattaaa agggcttaaa
     9841 gagatggatt tcggtatttt cgaggcacaa cctgaaatgc ttttgcccaa gtttcgtcca
     9901 ggagccaact catttgaaga cctcttggtg cctttcggag gggaggatat ccgatccgtg
     9961 ggccaacgag tagctcagac ggtagaagat acggtgtcag aacaggaaga aaacacggta
    10021 ttagctgtca gtcatggtgc agccatgtgg gggtttgtgt tgcatcacaa aatcgaactt
    10081 ccagctgatt tccgatttag caactgtgcc atttgccagt tagaatacga tggggaccag
    10141 attcgcttag tcaaagtaat caatcctgtc ctcaatcaag tgattgattt gtaaggaggg
    10201 ggaaaatggc aagaaaactg tacttaatgc gccatggaca aactcggttt aatcaacaag
    10261 gtcggattca aggggcctgt gattctcccc tgacagaact gggaagagag caagcactag
    10321 ctgctcacca gtattttcaa gagcaaggga ttgagttcga taaaatctat tcttcaaccc
    10381 aagaacgtgc ctgtgatacg gcagaattgg tcacagggag gacagattat acccgcttga
    10441 aaggcttgaa agaacaggat tttggagctt tcgagggtca acaggaatat cttaatcccc
    10501 ctctccaagg agatattggc tacggtaatt actttgtcac ctttggagga gagtcttatc
    10561 aagatgtccg acaacgaatg gtcgaaacca ttgggggcat catggaagaa gcagacaatc
    10621 aatctgtttt agtagttagc catggagctg ccatagccca atttttcagg caggtattga
    10681 cagattatcc gcaagttcgg atgcgtaatt gcgccatatt gacctttgat tatgaagatg
    10741 gaaagtatga cctactcagt gtcgttgacc ctgtgaacag agagattctt tatcaacaac
    10801 aaagctagga aaaggagaaa aaatgacagt aacaaaagta tttccagaag gatttttatg
    10861 gggtggtgca acagctgcca accaatgtga aggtgcttac gatgtagacg gacgtggact
    10921 tgccaatgtt gacgtagttc caattggtga agaccgtggt gccattatca ctggtcgtaa
    10981 gaagatgttt gactttgagg acggttattt ctatccagcc aaggagtcta ttgatatgta
    11041 tcatcacttc aaggaggata ttgccctctt tggtgaaatg ggttttaaga cctaccgtct
    11101 ttcaatcgcc tggtcccgta ttttccctaa gggagatgag ctagagccta acgaaaaagg
    11161 tctgcaattc tacgaggact tgttcaagga atgccacaag tacggcattg agcccctagt
    11221 gaccattacc cactttgact gccctatgca cttgattgag acctacggtg gctggcgaaa
    11281 tcgcaaaatg cttgaatttt acgaacgcct ttgccgtacc ctcttcacac gctacaaggg
    11341 tttggtcaaa tactggctaa ccttcaacga aatcaacatg attcttcatg caccatttat
    11401 gggggcaggt ctgtattttg aagaaggtga aaatgtggag cagatcaaat accaagctgc
    11461 ccaccacgag cttgtagcat ctgccattgc tactaaatta gcccatgaaa tcgatccgga
    11521 aaacaaggtc ggttgtatgt tggctgctgg tcaatactac ccacacacct gccacccacg
    11581 tgatgtctgg gaaggtctgc aagaagaccg tgagaactat ttctttatcg atgttcaggc
    11641 gcgtggctat tatccaaact atgccaagaa gaaatgggaa cgtgaaggta tcgagattga
    11701 aatgacagac caagatctgg cactcctgaa agagcatacg gtggacttta tttccttctc
    11761 ttactactct agccgcgttg catctggcga tccagcagag aaggaaaaaa cagcaggaaa
    11821 tatctttgct tccattaaaa acccttacct ggacgcatca gaatggggct ggcagattga
    11881 cccgctcggt ttccgcatta ccctcaattc catctgggat cgctatcaaa aaccactctt
    11941 tgtggttgaa aatggtctag gtgctgttga tacaccagat gaaaaaggtt atgtagaaga
    12001 tgattaccgt atcgactatc tccgtcaaca tatcctggct atgcgcgatg ctattgttga
    12061 agacggagtt gaactcttgg gctacaccac ttggggctgt attgacctgg tgtctgcagg
    12121 aacgggcgaa atgaaaaaac gctacggctt catttatgtc gatcgtgaca atgaaggaac
    12181 tggcaccctc aaacgctcta agaaaaaatc atttgaatgg tataagaaag tcatcgctac
    12241 aaacggagcg gatgtgggat aaaacatgag agacaggtct tcggacttgt cttttgttat
    12301 cgtttccact ataataaaaa ttagttaaca ataaaggagt tttgcatgaa atacatcgta
    12361 aacaacagca atgacccagc ttataacatt gccctcgaag cctatgcctt caaggaatta
    12421 acagatattg acgaaatttt tattctctgg atcaatgagc ctgccattat tatcggtaag
    12481 catcagaatg ctattgaaga aatcaacaag gaattcacag acgaaaaggg catccatgtt
    12541 gttcgccgtc tgtcaggtgg tggcgcagtt tatcacgacc taaataacct caactatact
    12601 attatttcaa acaaggcaga cgagggagcc tttgacttca aaaccttctc caagcctgta
    12661 attgataccc ttgcaacact gggtgtagag gccaatttta ccggtcgtaa tgacctggaa
    12721 atcgatggta agaaaatctg tggtaatgcc caagcctacg ctaaaggccg tatgatgcac
    12781 cacggttgct tgctctttga tgtggatatg tcagtcctcg caagtgccct caaggtcagc
    12841 aaggataaga ttgagtccaa gggtgtcaag tcagtccgtg cccgtgtgac caatatcaac
    12901 aacgaattgc cagagaagat gacagtcttg gagttcaaag atgctatcct caaccagatg
    12961 aagctagaat acccagacat ggacgagtat gtcttgtcag aaggtgactt ggctcgtatc
    13021 caagaaatcc gtgataccca gtttgcgaca tgggattgga cctacggtca aacaccagaa
    13081 tacactgtgg agcgtagcgt gcgttaccca gctggtaaaa tcacaaccta catcaaggct
    13141 gaaaaatcag tcatcgaatc tatcaagatt tatggagact tcttcggtat cggtgatgta
    13201 tcggatattg aagacttgct ggttggcacc cgctatgaat acgcagatgt cctagctaag
    13261 ttacaagaaa tcgacaccac gcactatttc tcacgcatga caacagaaga agtagcaaaa
    13321 gcgattgtag cttaatccat agggctggcc tttgtttggg cagtctttat tttttgtgaa
    13381 actgttttcg gcaaactgtt ttcacttgca cattttctat attatggtat aattattttc
    13441 tgtgagtatc cctcacttac tcgtggctag accatgagtc attagaccaa aaggaggaac
    13501 atatcaatgg ctaaatacga aattctttat attattcgtc caaacattga agaagaagct
    13561 aaaaacgctt tggtagcacg ctttgactct atcttgactg acaacggtgc aactatcgtt
    13621 gaatcaaaag catgggaaaa acgtcgcctt gcatacgaaa tcaaagattt ccgcgaaggt
    13681 ttgtaccaca tcgttaacgt tgaagctaac aacgatgaag ctcttaaaga gtttgaccgt
    13741 ttgtcaaaaa tcaacggcga cattcttcgt cacatgattg tcaaacttga cgcgtaagaa
    13801 ggtgttgtat gattaataat gtagtattgg ttggtcgtat gacccgtgat gcagaacttc
    13861 gttatactcc gtctaatcaa gctgttgcga cttttacttt ggcggttaac cgcaatttta
    13921 aaaatcaaaa cggtgagcgt gaagcggact ttatcaacgt agtcatttgg cgtcaacaag
    13981 ctgagaattt ggcgaattgg gctaagaaag gtgctctgat tggtgttaca ggtcgtatcc
    14041 agacacgtag ctatgacaat cagcaagggc aacgtgtcta cgttactgag gtagttgcag
    14101 aaagtttcca actcttggaa agccgtactg cccgtgaagg tcaaggtgga ggctattcag
    14161 ctggcaactc gtttgctgga ggaaataact acaactcacc ttatcaagcg cctgcacaat
    14221 ctacaccaaa cttcgctcga gaagaaagtc catttggagc aagtaatcca atggatatat
    14281 cagacgatga cctacc
//
LOCUS       DATUCG010000057        14281 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.058, whole genome shotgun sequence.
ACCESSION   DATUCG010000057 DATUCG010000000
VERSION     DATUCG010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14281
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.058"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            55..432
                     /locus_tag="VCA20_001345"
     CDS             55..432
                     /locus_tag="VCA20_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835159.1"
                     /translation="MRYFSPALVSVDYINQFKSQSKTVLAAVAISAAILGFAGGMSIS
                     KSSAGVSTQSQATLAQNGSVAKSGQVATQGMPSGQGTTPPDATSGATQSNSSTSSDEV
                     NALKEKNQEIKAQISGDSSSYNQ"
     gene            626..1297
                     /locus_tag="VCA20_001346"
     CDS             626..1297
                     /locus_tag="VCA20_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019320695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEP1835160.1"
                     /translation="MIKILLVEDDLSLSNSVFDFLDDFADVMQVFDGEEGVYEAETGV
                     YDLILLDLMLPEKDGFQVLKELREKGVTTPVLITTAKESLEDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRAGKFNENTLTFGDVTVDLSTNMTMVNGEEVELLGKEFDLLVY
                     FLQNQNVILPKTQIFDRIWGFDSDTTISVVEVYVSKIRKKLKGTTFGENLQTLRSVGY
                     ILKNA"
     gene            1290..2669
                     /locus_tag="VCA20_001347"
     CDS             1290..2669
                     /locus_tag="VCA20_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEP1835161.1"
                     /translation="MPKRFKKLMYTDKFSFFIRYFAVFTLIFGLMTAIIFQLMRSTMY
                     QNSDNTLKRIKKEPALAVGFAIARTYEPNSVFILQDSPTGEETTSSSSDSMPVPKNQK
                     NTRAGDQLRLGANTHVLLYSKSGEMINPDTFTGLADLSLDKEKLGEIKETTVESSFGM
                     SEDYRYITIGLATDELGYYSSYDIKYATILVNVSQIKSSIESYESTVAIVMVSAWLIS
                     ILASIYLSNLSMRPILISYQKQKDFVENASHELRTPLAVLQNRLESLFRHPETTILES
                     SESIGSSLEEVRNMRLLTTNLLNLARRDDGLKVDMIDVQPNYFDEIFANYLMIAEENG
                     KTLTVNNLIHQPIRTDKVLIKQLLTILFDNAMKYTDDDGTIQMAANIKDKLVCFTVID
                     NGLGISDADKKKIFDRFYRVDKARTRQKGGFGLGLSLAQQIIKNLDGEISVRDNQPKG
                     TIFEVRLPK"
     gene            2992..4220
                     /locus_tag="VCA20_001348"
                     /pseudo
     CDS             2992..4220
                     /locus_tag="VCA20_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637854.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase"
     gene            complement(4483..4731)
                     /locus_tag="VCA20_001349"
     CDS             complement(4483..4731)
                     /locus_tag="VCA20_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEP1835162.1"
                     /translation="MEVKPLANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANP
                     SEELFRAASSAIDKAETKGLIHKNKASRDKARLASKLA"
     gene            complement(4790..5710)
                     /locus_tag="VCA20_001350"
     CDS             complement(4790..5710)
                     /locus_tag="VCA20_001350"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009568566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEP1835163.1"
                     /translation="MKNEFLNFEQIDRATWQQLHRKTTIPLSQSELNSIKSFNDRIQL
                     HEVSDIYLPLVNLIHIYRKARKDLNFTKSLFLQKTIKPQPFIIGVSGSVAVGKSTTSR
                     LLQILIARTFKHAKVELVTTDGFLHPNAVLEERQLLNKKGFPESYDMEKLIDFLDKIK
                     NGYDCQIPVYSHEIYDIVPNKTQDIKSPDFLIVEGINVFQNPQNQRLYVSDYFDLSIY
                     VDADVEHIETWYLERFQKLLTLAKNDPNNYYHRFTQMTYPEILSIAQNTWKNINLANL
                     EKFIEPTRNRADIILHKAENHEIDKIYLKK"
     gene            5819..6409
                     /locus_tag="VCA20_001351"
     CDS             5819..6409
                     /locus_tag="VCA20_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEP1835164.1"
                     /translation="MSNMYYEKNPTIAHDIHEIHVNLLDTPMSFMTDAGVFSKKMVDY
                     GSQVLLKTLYFEKGASVLDVGCGYGPIGLTLAKVFSTKTTLIDINSRALDLATKNAER
                     NGVIAKIYQSNIYENVDETFNHIVSNPPIRAGKSVVHEVIAGAFQRLEAHGTLSIVIQ
                     KKQGAPSAKAKMEEVFGNCQIIKKDKGYYILESVKE"
     gene            6406..7683
                     /locus_tag="VCA20_001352"
     CDS             6406..7683
                     /locus_tag="VCA20_001352"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018543466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEP1835165.1"
                     /translation="MRAVDLIQKKRDGLELSSEEIKWLIDGYVAGTVPDYQMSALAMA
                     IYFKGMSTREISDLTMSMVATGEQIDLSAIPGIKVDKHSTGGVGDKVTLILAPLVASF
                     DIPVAKMSGRGLGHTGGTLDKLESIKGYQIEVSQADFIKQVQDTGVAVIGQSDNLVKA
                     DKLLYALRDVTATVDIIPLIASSVMSKKIAAGADAILLDVTVGEGAFMKTVEEARVLA
                     QTMVDLGKAVGRKTVAVLTDMSQPVGTSIGNRLEILEAIEILQGKGREDVTEFICELA
                     QIMLGLANVEKSIEEVRQHIDNGAALKKFEEMVRAQGGDLEDLYRPVQVTQQVPVLAE
                     EDGYIVGLPALEFGLFAMKLGAGRAVKTDNLDYETGIVFHKKVGEAVSKGEQIATIYA
                     NENISENMLTNFQKNVKIDKESVKTKEIIEIIS"
     gene            7699..8361
                     /gene="deoC"
                     /locus_tag="VCA20_001353"
     CDS             7699..8361
                     /gene="deoC"
                     /locus_tag="VCA20_001353"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEP1835166.1"
                     /translation="MKLNKYIDHTILKPETTQEQVEKILAEAKEYDFASVCVNPTWVA
                     LAAESLKDSDVKVCTVIGFPLGANTPAVKAFETKDAISNGADEIDMVINIGALKTGNY
                     DLVLEDIKAVVAASGDKLVKVIIEACLLTDDEKVKACQLSQEAGADYVKTSTGFSTGG
                     ATVADVALMRKTVGPDMGVKASGGARSYEDAIAFIEAGASRIGASSGVAIMNGAQADG
                     DY"
     gene            8348..8737
                     /locus_tag="VCA20_001354"
     CDS             8348..8737
                     /locus_tag="VCA20_001354"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEP1835167.1"
                     /translation="METIDLIELAVKNSQKAYVPYSKFPVSAVLVAKTGEIFTGVNIE
                     NASFGLTNCAERTAIFKAISEGVKDFSEIIIYGKTEKPISPCGACRQVMAEFFDKDLK
                     VTLVAKDKSTVETTVGELLPYSFTDLT"
     gene            8818..9885
                     /locus_tag="VCA20_001355"
     CDS             8818..9885
                     /locus_tag="VCA20_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEP1835168.1"
                     /translation="MNKKLVGLGLAAVAAVSLAACGNRASKSSSSEASSSADTSVKAA
                     IVTDIGGVDDRSFNQSAWEGLQEWGNVAGLSKGNGYDYFQSANESEYITNLDSAVAGG
                     YNLVFGIGFALESAIAEVAPNNPDTHYVIVDSVVPDQDNVVSVGFADHEASYLAGVAA
                     AKSTKTNHVGFIGGMEGVIIDRFEAGFVAGAKSVNKDIKITVDYAGSFGDAAKGQTLA
                     AAQYAAGADVIFHASGGTGNGVFAAAKAENETRNEADKVWVIGVDRDQSAEGTYTSKD
                     GKSSNFVLVSTLKQVGTSVKDIATKAAAGDFPGGQVLQFSLKDKGVELAETNLSEDAS
                     KAVADAKQAILDDKVEVPEKP"
     gene            10080..11615
                     /locus_tag="VCA20_001356"
     CDS             10080..11615
                     /locus_tag="VCA20_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1835169.1"
                     /translation="MTKDYVIEMRNITKIFGEFVANDHINLNVRRGEIHALLGENGAG
                     KSTLMNMLAGLLEPTSGEIVINGQSVKIDSPSKAAQLGIGMVHQHFMLVDAFTVAENI
                     ILGSETTKGGVIDLTKAIAEIKELSAKYGLEVDPTAKVADISVGAQQRVEILKTLYRG
                     ADLLIFDEPTAVLTPAEIAELLKIMKALVEEGKSIILITHKLDEIRAVADSVTVIRRG
                     KSIETVAVEGATNQDLAEWMVGRSVSFKTEKVEASPKEVILSIKDLVVNENRGIPAVK
                     GLSLDVRAGEVVGIAGIDGNGQSELIQAITGLRKVKSGSITIKGEEVVGKTPRKITEM
                     QVSHVPEDRHRDGLVLPMTVAENFALQTYYKEPISKNGILNYNIINEKARVLMEEFDV
                     RGASELVPIKALSGGNQQKAIIAREIDRNPDLFIVSQPTRGLDVGAIEYIRKRLIAER
                     DKGKAVLVVSFELDEILDVSDRIAVIHDGVIHGIVEPAKTNKQELGVLMAGGHIEKGE
                     EHA"
     gene            11608..12669
                     /locus_tag="VCA20_001357"
     CDS             11608..12669
                     /locus_tag="VCA20_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP1835170.1"
                     /translation="MLNKYKNLALPLMAVVSGILLGAIVMLVFGYDPIWGYEELFYSA
                     FGNIKSIGEIFRAMAPLVFTALGFAVASRAGFFNVGLSGQAYVGWVFAGWFALANPDL
                     PRPVLILGTVLIAMLAGGIAGAIPGILRAYLGTSEVIVTIMMNYILLYSCNYIIRDVF
                     ADSLMKNTDSTVNVSANASYQTEWLRALTDNSRMNIGIFFAIIAVFVIWFLLTKTTLG
                     FEIRSVGLNPTASDYAGMSAKRTIILSMIISGALAGLGGAIQGLGTFQNVYIQSGNLD
                     IGFNGMSVALLASNSPLGIPLAAFLFGVLSVGAPGMVRAQIPPELINVVTASIIFFIG
                     VKFIFEQLLKSRNKAKGAK"
     gene            12671..13624
                     /locus_tag="VCA20_001358"
     CDS             12671..13624
                     /locus_tag="VCA20_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP1835171.1"
                     /translation="MNVSILALLISQMLIYSAPLIFTSLGGVFSERGGIVNVGLEGIM
                     VIGAFAGVVFNIEFAGTFGNATPLLAVIVGGLAGVVFAAIHAMATINFRADHVVSGTV
                     LNLLAPALGVFLVKVIYNKGQTDSITESFGKFSFPILADIPVIGEIFFKNTSLMGYVA
                     IATAFLAWFILYKTKFGLRLRSVGEHPQAADTLGINVYAMRYAGVLIAGFLGGVGGAV
                     SAQSVNINFSATTIVGSGFIALAAVIFGKWNPIGAMLASLFFGLSQSLAVVGGQLPGL
                     KDIPTVYLQIAPYLITVIALSAFFGKAVAPKADGINYIKSK"
     gene            13678..>14281
                     /locus_tag="VCA20_001359"
     CDS             13678..>14281
                     /locus_tag="VCA20_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEP1835172.1"
                     /translation="MSYSKEEELEGLLVENRENDWYNSLLLRYKMRLIVRRLVLNIAV
                     SLDGFIARKDGSYDWIEGHGTDKYDTVLQFDNQKFFNNCDTVVMGRKSLEDCPLEMIE
                     GYQEKQFIVASHTEQTDYGNVRFVRDIIGEIMELRSREGGDIWLFGGASLVQDCLEAG
                     VIDHLIIGIIPTILGDGIPLFASLLEEKKLLLVESTVTDGI"
BASE COUNT         4279 a         2374 c         3184 g         4444 t
ORIGIN      
        1 gggctcacat caaaaaacac ctcagaagag tattgccaaa ttgcgatact tttcttgagg
       61 tattttagtc ctgctcttgt ttcagttgac tatattaatc aatttaagtc acagtcaaaa
      121 acagtactag ctgctgttgc tatttcagct gctatcttgg gctttgcagg gggaatgagt
      181 atttctaaaa gctcagcagg agtttcaaca caaagtcagg caactttggc gcaaaatggt
      241 tccgtggcta agagtggaca agtagctacc caaggcatgc catctggaca aggtacaact
      301 cctcctgatg cgacaagtgg agcaacccag tcaaactcga gtacttcttc tgatgaagtg
      361 aatgctttaa aagagaagaa tcaagaaatt aaagctcaaa tttcaggaga tagtagttct
      421 tataatcaat aaatattgag atttcgattt ttatttattc catgatataa tgaactcgta
      481 tcaataaata gttagataaa gactacctta tcattcattt tttagatggt catagtagta
      541 ctttatttaa ccatttctct taaagaccgt gacctgtccc aggtctttgg tttgtttatt
      601 aaaccgaaat gtaaggaagt gtatgatgat taaaatattg ttagtggaag atgatttaag
      661 cctatcaaat tctgttttcg atttcttaga tgattttgcg gatgttatgc aggtttttga
      721 tggtgaagag ggcgtttatg aggcggaaac aggcgtttat gatttgattc ttttggattt
      781 aatgttacct gaaaaagacg gctttcaagt cttgaaagaa ttgcgtgaaa aaggagtgac
      841 aactcctgtc ttgattacaa cagccaagga aagtcttgag gataaaggtc atggatttga
      901 attaggtgca gatgattatt tgaccaagcc attttattta gaagaattga agatgcgaat
      961 tcaagctcta ttaaaacgag cgggtaagtt taatgaaaac acactaacct ttggagatgt
     1021 gacagttgac ctatctacaa atatgacgat ggtaaatggc gaagaagttg aactgctagg
     1081 aaaagaattt gacctactag tttatttctt gcaaaatcaa aatgttattt tgccaaaaac
     1141 acaaattttt gaccgtattt ggggctttga cagtgatact acaatttcag tagtagaagt
     1201 atatgtttct aaaattagga aaaagttgaa aggaacgact tttggggaaa atcttcaaac
     1261 cctacgtagt gtcggataca ttctaaaaaa tgcctaagcg atttaaaaaa ttaatgtaca
     1321 cggataaatt ttctttcttt atccgttatt ttgcagtatt tactctgatt tttggtttaa
     1381 tgacagccat catttttcaa ttaatgcgtt caactatgta ccaaaattca gataacacct
     1441 tgaaacgcat taagaaagaa ccagcgctag ctgttggttt tgcaatcgca agaacctatg
     1501 agcccaattc tgtatttatc cttcaagata gtccaactgg tgaagaaact acgagctcta
     1561 gttcagatag catgcctgtt cctaaaaatc aaaaaaatac tagagctgga gatcagctga
     1621 gattaggtgc caatactcat gttttactct atagtaaaag tggagagatg attaatccag
     1681 atacttttac tggtttggcc gacctatcat tagacaagga aaaattgggt gaaattaagg
     1741 aaactacggt tgaatcaagt tttggtatgt ctgaagatta tcgctatata acgattgggc
     1801 tggctacaga tgaactaggt tattattcgt cctatgatat aaaatatgcg acaattttgg
     1861 tgaatgtcag ccaaattaaa tcttccattg agagctatga atcgacagtt gctattgtta
     1921 tggtatcagc ttggttgatt tctatactag caagtattta cctatcgaat cttagcatgc
     1981 gtccaatcct aattagttat caaaaacaaa aagacttcgt tgaaaatgct agtcatgagt
     2041 tacgcacacc gttggcagtt cttcaaaatc gcctggaaag tctatttcgt catcccgaga
     2101 caactatttt ggaaagtagt gaaagcatcg gatctagttt agaagaagtt cgaaatatgc
     2161 gactattgac aacaaattta cttaatttgg ctcgtcgtga tgatggctta aaagtcgata
     2221 tgatcgatgt ccaacccaac tattttgatg aaatctttgc caattacctt atgattgccg
     2281 aagaaaatgg aaaaactcta acagttaata atttgattca tcagccaatt cgaacagaca
     2341 aagttttgat taaacagttg ctcaccattt tgtttgacaa cgctatgaag tataccgatg
     2401 atgatgggac aattcaaatg gcagctaata tcaaggataa actagtttgt tttacagtta
     2461 ttgacaatgg tttgggaatc agcgatgccg ataaaaagaa aatctttgat cgcttttacc
     2521 gagttgacaa ggctagaaca cgtcaaaaag gtggatttgg tttaggttta tctttagctc
     2581 aacaaattat taagaatcta gatggtgaga tttctgtgag agataaccaa ccgaaaggaa
     2641 ctatttttga ggtacgttta cccaaataac taaccattca tcaagattag aggctgggta
     2701 aaaaatccag cctttcttct caaagttcgc tctatttcca acttccaact gtctcccaga
     2761 tagttttgta gcgaactctg ttcgctctat ctccagcctc caaaggtttt gggaactgtt
     2821 tcagctggtc acctaaaaat aagaaagtga ccatagaaaa ttgaacctaa atgtttggaa
     2881 gttcggggaa ctcttttcga tctattgaat tctttccact ccctttttgt tttgtgtagt
     2941 ttgtcagatg cttcctaatt atgttatact taaacaagaa tggaggtaaa tatgaggaaa
     3001 atttctcgtg gcattttaac catattatta tcggcgacag tcctttctgc ttgttcagga
     3061 ggaggacaag agctgagtga acgtttggaa gcagctaaaa ttgaattttc taaagaaaaa
     3121 aagcaactag aggcagaatt aacagcatta aaagaagagg ttcaaggaaa tttttactac
     3181 caacaattgg atactgataa ggaacgtcgt gtctatctcc aatttgttaa tgggctcagc
     3241 aagcggatgc aagtgatcga tattgattct gttaatgatg aaatatattc gcgcgtctat
     3301 ttttctgtcg ctcatgatta tccagaattt tactggctaa cagatgaagc agcaatggct
     3361 ggtggaattg atatattgga tttagaagag ccagtgtatc cgactgattt atctccgaca
     3421 cgaaataaaa tagaagagga agtgaataag atacttgctc aggcaccaac agggtcgtac
     3481 tatgagaagg ttaaatattt ttacgaggtg attataaagc aaacggatta cgaccttgaa
     3541 gccctacaaa ctaactctgt cacgtggaga agccaaggaa ttgccagtgt gctattagat
     3601 aagaaatccg tttgtgctgg ctatagtcgc accttccaat atctatgtaa aaaagcaggc
     3661 attgattgta tctatgtcac agggattgca aaaaacggac aaaacggtga gtttgggcat
     3721 gcttggaatc tagtcaagat aaatggacaa tactatggtg ttgatacgac ttggggagat
     3781 ccagtttttt atcaagctat cagtggagaa gcgcatacag atatttcata cgattatcta
     3841 tgtgtcctga tgaaatatta gagagaagta gaattgctga taccgaccta ctcgactact
     3901 ggggagaaga acactattac gaacctagag cgttgaccta tccaaagtgt acagataatt
     3961 cattgaatta ctatgttcaa aagggagttt atttcacaag ctttgatgaa gctgcagttt
     4021 tacagtccat cactgaccag cgactacaag gtacggataa agtagtcttg caattcggaa
     4081 cagcagaatt aatgcaacaa atgattacat tggcctctac tgaaaataat gctatttttc
     4141 aggctttagg cgatgtagga gagtatcagt actattataa tgaccaaacc tatacatttg
     4201 aactggcaga ttggttttaa ggaattagtt tgaatctcca ggagcgtgtt cttccctaat
     4261 aataaaaaat agggagtggg aaagacattc atagtggctc cgccctcacc cacagaataa
     4321 tgaaaacgaa cataaagccc tcactaggtg tataatctta gtgagggctt tacggttgac
     4381 gagttaatga tgttactcat cgagggaaaa ctgttccact ttgtcccgcc tccttatgat
     4441 ggaaaaaata aaaaaaagga aacccaaagg tttctttttt aattaagcaa gttttgatgc
     4501 aagacgtgct ttatcgcggc ttgctttgtt tttgtgaatc aaacctttag tttctgcttt
     4561 atcgattgct gaactagcag cacggaaaag ctcttcagat gggttagctt caaatgcttt
     4621 gattgcagta cgcatagctg atttttgtgc tgagtttttc tcgttttgtt taacgttcaa
     4681 ttcagcgcgt ttgatagctg acttaatgtt tgccaatggt ttcacctcca ccctttcttg
     4741 tactaactat accattatat ctgaaaagag tatttttgac aagtgaaatt tattttttta
     4801 gataaatttt atcaatctca tggttttcgg ccttatgaag aataatatca gcacggtttc
     4861 tggttggttc aatgaatttt tctaagttag ctagattaat atttttccaa gtattctgag
     4921 caatagatag aatctcaggg tacgtcatct gggtaaaacg atggtaatag ttgttagggt
     4981 cattttttgc caatgtcaat aatttttgga atcgctccaa gtaccaggtt tcaatatgct
     5041 ccacatcagc gtcaacgtat atagacaggt caaaatagtc actgacatag aggcgctggt
     5101 tttgtggatt ttgaaataca ttgattcctt caacaatcaa gaaatctgga gacttaatat
     5161 cctgagtttt gttggggaca atatcataga tttcatgtga atagacagga atttggcagt
     5221 cgtagccgtt ttttatctta tcaaggaaat caatcaattt ctccatgtca taggattcag
     5281 gaaaaccttt tttatttaac agttgccgtt cttctaagac cgcatttgga tggagaaaac
     5341 catctgttgt cactaattca accttggcgt gtttaaatgt ccgagcgata agaatttgta
     5401 gcaatctact agttgttgat ttgccgacag caacgcttcc ggaaacgcca atgataaatg
     5461 gttgaggctt gatagttttc tgtagaaata aactcttcgt aaaatttagg tctttgcgtg
     5521 ctttccgata gatatggata agattaacca aaggaagata gatgtcagaa acctcatgta
     5581 attgaatgcg gtcgttaaaa cttttaatag aatttagttc gctttgacta agtggaatgg
     5641 tagtcttacg gtgtagttgt tgccaagtgg ctcggtcgat ttgttcaaaa tttaaaaatt
     5701 cgtttttcat acataattct tcctataggt aagcgtttca atatagattt cattttatca
     5761 tatttttcat gtaagaaaaa ctcttgaaaa cgtttttagg aaatgttata ataagtacat
     5821 gtccaatatg tattacgaaa aaaatcctac aatagcacat gatattcatg aaatccacgt
     5881 aaacttacta gacaccccaa tgtctttcat gacagatgca ggtgttttta gcaagaagat
     5941 ggttgattat ggtagtcaag tgcttttaaa aaccctttat tttgaaaaag gggcatcagt
     6001 gttggatgtt ggctgtggtt atggaccaat tggtttgaca cttgctaagg tgtttagcac
     6061 caagacaaca ctgattgata ttaatagcag agctttggat ttagctacaa aaaatgcaga
     6121 gaggaatggt gttatcgcta aaatttatca atcaaatatc tacgagaatg tggacgaaac
     6181 atttaatcat attgtttcaa atccacctat tcgggccgga aaatcggttg tacatgaagt
     6241 aattgctggg gcctttcaac gcttagaggc acacggaacc ttgtccattg tcattcaaaa
     6301 gaaacaaggt gctccgagtg ctaaagctaa gatggaagag gtgttcggca actgccagat
     6361 tataaagaaa gataagggtt actacatcct tgaaagtgtg aaagaatgag agcagttgat
     6421 ttaattcaaa aaaagcgtga tggcttagaa ctatcgtctg aagaaatcaa atggttgatt
     6481 gatggatatg tagctgggac agtaccggat tatcaaatgt cagccttggc gatggcgatt
     6541 tattttaaag ggatgtcaac acgtgagatt tctgatttga caatgtccat ggttgcaacg
     6601 ggtgaacaga ttgatttgtc tgcaattcca ggcatcaagg tagataagca ttctacaggt
     6661 ggtgtcggag ataaggttac actcatatta gcgccattgg tagcaagttt tgatattcca
     6721 gttgcaaaga tgagcggtcg tgggcttggt catactggtg gaaccttgga taagttagag
     6781 tcaatcaaag gataccagat tgaagttagc caagcagatt tcatcaagca agttcaagat
     6841 actggtgttg cagttattgg tcagtcagat aatcttgtca aagctgataa actactctat
     6901 gcccttcgtg acgtgacagc aaccgttgat attattccgc tgattgccag ctcagtaatg
     6961 tctaagaaaa ttgccgcagg tgcagatgcg attcttctag atgtcacggt gggagaaggt
     7021 gccttcatga agactgttga agaagcacgg gttctagctc aaactatggt ggatcttggg
     7081 aaagcggttg gtcgtaagac agttgctgtt ttaactgaca tgtctcaacc agttggaact
     7141 tcgattggaa atcgtttgga aattttagag gctattgaaa ttttacaggg taaaggtcga
     7201 gaagatgtta ccgaatttat ctgtgaacta gcccaaatta tgttggggct tgctaatgtt
     7261 gagaaaagca ttgaagaagt acgccaacat attgacaatg gtgcagcatt gaagaaattt
     7321 gaagagatgg tccgtgctca aggtggtgac ttagaagatt tatatcgccc agttcaggtt
     7381 acacagcaag ttccagttct tgcggaagag gatggctata ttgtggggct tcctgctttg
     7441 gaatttggtc tctttgcaat gaaacttggt gcaggtcgtg cagtcaagac tgacaattta
     7501 gattatgaaa caggtattgt tttccataag aaagtggggg aagctgtttc taaaggcgaa
     7561 caaatcgcaa cgatttatgc caatgaaaat atttcggaaa acatgcttac aaatttccaa
     7621 aaaaatgtta aaatagataa agaaagtgta aaaacgaaag aaatcatcga gattatttct
     7681 taatgtacgg agaaagctat gaaattgaat aaatatattg accacacaat cttgaaacct
     7741 gagacaacac aagagcaggt tgaaaaaatt ttagcagaag caaaagaata tgattttgca
     7801 agtgtctgtg ttaacccaac ttgggtcgct ttagcagctg aaagcttgaa agatagtgat
     7861 gtaaaagttt gtacggttat tggtttccct ctcggagcaa acacacctgc tgtaaaagca
     7921 ttcgaaacaa aagatgcaat ttcaaatgga gcagatgaga ttgacatggt tatcaatatc
     7981 ggtgctctga aaactggaaa ttacgatttg gttttggagg atattaaagc tgttgtagca
     8041 gcaagtggtg ataaactagt caaagttatc atcgaagcat gcctcctgac tgacgatgag
     8101 aaagtgaagg cttgtcaatt atctcaagaa gctggtgcgg actatgttaa aacatctaca
     8161 ggattttcta caggtggtgc tacagtagcg gatgttgcct tgatgagaaa aacagtaggt
     8221 cctgacatgg gagtgaaggc ttctggtggt gcacgttcgt atgaagatgc tattgctttt
     8281 atcgaagcag gagcgtctcg tattggtgcg tcatcaggcg tggcaatcat gaatggagca
     8341 caggcggatg gagactattg atttaattga attagctgta aaaaacagtc aaaaagcata
     8401 cgtaccgtac tcaaagtttc cagttagtgc agttcttgta gctaagactg gggaaatatt
     8461 tacaggtgta aacattgaaa atgcaagttt tggtttgacc aattgcgctg aaaggacagc
     8521 tattttcaag gctatttctg aaggtgtgaa agatttttct gaaatcatta tttatggtaa
     8581 aacagaaaaa ccaatatctc cttgtggagc ttgtcgccaa gttatggcag aattttttga
     8641 taaggattta aaagtgacct tggtcgctaa agataaatcg acagtcgaga cgacagtcgg
     8701 ggaattacta ccatattcct ttacagactt aacatagtct gtatgacagt catttgactg
     8761 tagcaatatt actctgcaac aatttagttg caaaaagatt tttaggaggg ttcagaaatg
     8821 aacaagaaac ttgtaggact tggtctagca gctgttgcgg ctgtatcttt ggctgcatgt
     8881 ggtaaccgtg cttcaaaaag cagtagctca gaagcatctt catcagcgga cacatctgta
     8941 aaagctgcta tcgttactga cattggtggt gttgatgacc gttcatttaa ccaatctgca
     9001 tgggaaggtc ttcaagaatg gggtaatgta gctggtcttt ctaaaggtaa tggctacgat
     9061 tacttccaat cagctaatga atcagaatac attacaaacc ttgattcagc tgttgctggt
     9121 ggttacaacc ttgtgttcgg tattggtttt gccttggaga gtgcgattgc tgaggttgca
     9181 ccaaataatc ctgacacaca ctatgtaatc gtggatagtg ttgttccaga tcaagacaac
     9241 gtagttagcg taggtttcgc agaccatgaa gcatcatatc ttgctggtgt tgctgcagct
     9301 aagtcaacta aaacaaacca cgtaggtttc atcggtggta tggaaggtgt tatcatcgat
     9361 cgctttgaag ctggtttcgt tgcgggtgct aagtctgtaa acaaagacat caagattaca
     9421 gttgattatg caggttcatt tggtgatgct gctaaaggtc aaactcttgc agctgctcaa
     9481 tatgctgcag gtgcagacgt gatcttccac gcttctggtg gtactggtaa cggtgtattt
     9541 gctgctgcta aagcagaaaa tgaaactcgt aatgaagcag ataaagtttg ggtaatcggt
     9601 gttgaccgtg accaatcagc agaaggtaca tacacttcta aagatggtaa gtcttctaac
     9661 tttgtattgg tttcaacatt gaaacaagtt ggtacttcgg ttaaagatat tgcaactaag
     9721 gcagctgcag gtgatttccc tggcggtcaa gtacttcaat tctcattgaa agataaaggt
     9781 gttgaattgg cagaaactaa tctttcagaa gatgcttcta aagcagttgc agatgctaaa
     9841 caagcaattt tggatgacaa agtagaagtt cctgaaaaac cttaatgaat ttcttttcga
     9901 caattccgat tcggcgaccc gacggggcgc cgttttcggg ctgagatttt tctcagtaaa
     9961 gtctatcagc tatggtaggt tttactgaca aaaatggtat agttgatgaa gagtttttaa
    10021 gtactcaaag tagtactgaa gaattttcag aaggctatag tcagaaagga taatagatta
    10081 tgactaagga ttatgtcatt gaaatgcgca atatcactaa gatatttggt gaatttgtag
    10141 caaatgacca catcaatttg aatgtcagac gtggtgaaat ccatgccctt cttggtgaga
    10201 atggtgcggg taaatctact ctgatgaaca tgcttgctgg cttgttggaa cctacaagcg
    10261 gtgaaattgt tatcaacggt caatccgtga agattgactc tccatccaaa gcagcccaat
    10321 tgggtattgg tatggtgcac caacatttta tgttggttga tgctttcaca gttgcagaga
    10381 acatcattct tggttctgaa accaccaaag gtggcgtgat tgaccttaca aaagctatcg
    10441 cagaaattaa agaactttct gctaagtatg gtcttgaagt tgatccaact gcaaaggttg
    10501 cagatatttc tgtgggtgca cagcaacgtg tagaaatcct taaaacactt taccgtggag
    10561 cagacctttt aatttttgat gagccgacag ctgtattgac ccctgcggaa attgcagagc
    10621 tcttgaaaat tatgaaagcc ttggttgaag aaggtaaatc tattattttg attacgcaca
    10681 aattggatga aattcgtgca gttgccgata gtgtaacagt tattcgtcga ggtaaatcaa
    10741 tcgaaacagt tgccgttgaa ggagcaacaa accaagattt ggctgagtgg atggttggac
    10801 gttccgtttc ctttaaaaca gagaaagttg aagcgagtcc taaagaagtc attttgtcaa
    10861 ttaaggattt ggttgtcaat gaaaaccgtg gtatcccagc agttaagggc ttatcgcttg
    10921 atgttcgagc aggtgaggtt gttggtattg ccggtatcga cggaaacggt caaagtgagt
    10981 tgattcaggc aatcactggt ttacgtaagg taaaatctgg ctcaatcact atcaaaggtg
    11041 aggaagtagt tggtaaaact ccacgtaaaa ttacagaaat gcaagtgagc cacgttcccg
    11101 aagatcgtca tcgcgatgga cttgttcttc caatgactgt tgctgagaac tttgcgcttc
    11161 aaacttacta taaagaaccg atttcaaaaa atggtatctt gaactataat atcattaatg
    11221 aaaaagcacg cgtgttaatg gaagaatttg acgttcgtgg ggcgagtgaa ctcgttccaa
    11281 tcaaggccct ttcaggcggt aaccagcaaa aagctatcat cgcgcgtgaa atcgatcgca
    11341 acccagacct tttcattgtt agccaaccaa cacgcggttt ggacgttggt gctattgagt
    11401 atattcgtaa gcgtctcatt gcagaacgtg ataagggtaa ggctgttctt gttgtcagct
    11461 tcgaattgga tgaaattttg gatgtatcag atagaattgc tgttatccat gatggtgtca
    11521 tccatggtat tgttgaacct gctaagacca ataaacaaga gttaggtgtc ttgatggcag
    11581 gtggacatat tgagaaggga gaagaacatg cttaataaat ataagaattt ggcccttcca
    11641 ttaatggctg tggtcagtgg tatcctactt ggagcgattg tcatgcttgt ttttggctac
    11701 gatccaattt ggggctatga agagttattc tattcagctt ttggtaatat caaatcaatt
    11761 ggtgaaattt tccgtgcaat ggcaccactt gttttcactg ctctcggttt cgcagtagct
    11821 agtcgtgcag gtttcttcaa cgttggtttg tctggtcagg cctatgttgg ttgggttttt
    11881 gctggatggt ttgccttggc aaatcctgac ttaccacgtc cagttttgat tttgggaaca
    11941 gtcttgattg ctatgttggc aggcggtatt gctggagcta ttccaggtat tctacgtgcc
    12001 tatttgggaa caagtgaagt tatcgttacg attatgatga actacatctt actctattca
    12061 tgtaactata tcattcgtga tgtatttgca gatagtttga tgaagaatac agactcgaca
    12121 gttaacgtgt cagccaatgc ctcttaccaa acagagtggc tccgtgcatt gacagataac
    12181 tcacgtatga atattggtat tttctttgcc attattgcag tttttgtgat ttggttccta
    12241 ttgaccaaaa cgactcttgg ttttgagatt cgctcagtgg ggcttaaccc aacagcatcg
    12301 gattatgcgg gtatgtctgc gaaacgtacg attattctat ctatgattat ttcaggtgct
    12361 cttgcgggtc ttgggggagc tatccaaggt ttgggtactt tccaaaacgt ttatatccaa
    12421 agcggtaact tggatattgg ttttaatggt atgtcagtag cgcttttagc ttctaattca
    12481 ccgttaggta ttccattggc tgcctttcta ttcggtgttc tttctgtcgg tgctccaggt
    12541 atggtacgtg cacagattcc acctgaatta atcaacgttg ttacggcatc aatcattttc
    12601 tttatcggtg tgaaatttat ttttgaacaa ttattaaaat ctagaaataa agcgaaagga
    12661 gcgaaataag atgaatgttt caattcttgc cttactcatt tcgcaaatgt tgatttattc
    12721 agcaccctta atctttacca gtcttggagg agttttctct gaacgcggtg gtattgtaaa
    12781 cgttggtcta gaaggaatca tggttatcgg tgcctttgct ggtgttgttt tcaatatcga
    12841 atttgcaggg acatttggta atgctactcc attattagct gttattgtag gaggtcttgc
    12901 tggtgttgtc tttgcagcta ttcatgcgat ggcgacgatt aatttccgtg cagaccacgt
    12961 tgtatcaggt acggtgttga accttttggc tccagcattg ggagttttcc tagtcaaggt
    13021 tatctataat aagggtcaaa ctgatagtat tactgaatca tttggtaaat tttcattccc
    13081 tatcttggca gatattccag tcattggtga aattttcttc aaaaatacaa gcttgatggg
    13141 ttatgtagct attgcaactg catttcttgc ttggttcatc ctttataaaa ccaagttcgg
    13201 tttgcgcctt cgttcagtag gtgaacatcc gcaggcagca gatactttgg gaatcaatgt
    13261 ctacgctatg cgttatgcag gtgtgttgat tgctggtttc cttggtggtg ttggtggagc
    13321 tgtaagtgct caatcagtga atatcaactt ctctgcaaca acaatcgtag gttcaggttt
    13381 catcgctcta gcagctgtaa ttttcggtaa gtggaatcca attggcgcta tgcttgccag
    13441 tctattcttc ggtctttcgc aaagtttggc agtagtgggt ggacaattac caggattgaa
    13501 agatattcca acggtttatc ttcaaattgc accatacttg attactgtta ttgctctgtc
    13561 tgctttcttt ggtaaagcag tcgctcctaa agcagacggt atcaactata tcaaatctaa
    13621 ataaaaaggt ccagtggacc tttttatcat gttctttgct gtacaggaaa tgaataattg
    13681 tcctattcaa aggaagagga gttagaggga cttttggttg agaataggga gaatgactgg
    13741 tataatagct tgttactaag gtataaaatg aggttaattg tgagacgatt ggtgttaaat
    13801 attgcagtga gtttagatgg atttattgca cgtaaggatg gaagctacga ttggattgaa
    13861 ggacatggta ccgataaata cgatacagtc ttacagtttg ataatcaaaa attctttaac
    13921 aactgtgata cagttgtgat ggggagaaaa tcattagaag attgtccgtt ggaaatgatt
    13981 gaaggatatc aggaaaagca atttatcgtt gcgagtcata ccgagcagac ggactatggt
    14041 aatgtgcgtt tcgtaaggga catcatcggg gagattatgg aacttcgttc tagagaaggt
    14101 ggagatattt ggttgtttgg tggagcaagt ttggttcagg actgtttaga ggcgggagtg
    14161 attgatcacc ttattattgg gattattcct actattttgg gtgacggtat tccacttttc
    14221 gcctcacttt tggaagaaaa gaaattgctc ttggtagagt caactgtcac agatggaatt
    14281 g
//
LOCUS       DATUCG010000058        14162 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.059, whole genome shotgun sequence.
ACCESSION   DATUCG010000058 DATUCG010000000
VERSION     DATUCG010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14162
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.059"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            1..866
                     /locus_tag="VCA20_001360"
                     /pseudo
     CDS             1..866
                     /locus_tag="VCA20_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921629.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            876..1079
                     /locus_tag="VCA20_001361"
     CDS             876..1079
                     /locus_tag="VCA20_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HEP1835173.1"
                     /translation="MYQLGSFVEMKKPHACVIKSTGKKANKWEVIRLGADIKIRCSNC
                     DHVVMMSRHDFERKMQQVLPSET"
     gene            1183..1542
                     /locus_tag="VCA20_001362"
     CDS             1183..1542
                     /locus_tag="VCA20_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP1835174.1"
                     /translation="MKQLAQQIRVLRTAKNLSQDDLAEKLYISRQAVSKWENGEATPD
                     IDKLVQLAEIFGVSLDYLVLGKEPEKEIVVEQRGKMNVWEYLNEESKRPLTRGDVVVL
                     IFLAVMLLGGLFIQHYF"
     gene            1627..2742
                     /gene="ychF"
                     /locus_tag="VCA20_001363"
     CDS             1627..2742
                     /gene="ychF"
                     /locus_tag="VCA20_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEP1835175.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTELITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPIADIDTINLELILADLESINKRYARVEKMARTQKD
                     KDSVAEFAVLEKIKPVLEDGKSARTVDLTDEEQKIVKQLFLLTTKPVLYVANVDEDKV
                     ADPESISYVQQIRDFAATENAEVVVISARAEEEISELDDEDKDEFLEALGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGMKAPQCAGIIHSDFEKGFIRAVTMSYDD
                     LIQYGSEKAVKEAGRLREEGKEYVVQDGDIMEFRFNV"
     gene            2900..3472
                     /locus_tag="VCA20_001364"
     CDS             2900..3472
                     /locus_tag="VCA20_001364"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEP1835176.1"
                     /translation="MTRLIIGLGNPGDRYFETKHNVGFMLLDKIAKRENVTFNHDKIF
                     QADIATTFIDGEKIYLVKPTTFMNESGKAVHALMTYYGLDATDILVAYDDLDMAVGKI
                     RFRQKGSAGGHNGIKSIVKHIGTKEFDRIKIGIGRPKGKMSVVNHVLSGFDIEDRIEI
                     DLALDKLDKAVNVYLEEDDFDTVMRKFNGQ"
     gene            3469..6963
                     /gene="mfd"
                     /locus_tag="VCA20_001365"
     CDS             3469..6963
                     /gene="mfd"
                     /locus_tag="VCA20_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEP1835177.1"
                     /translation="MNILDLLHKNKQINQWQSGLTKSTRQLLLGLTGTSKSLVMATAY
                     DSMAEKIMIVTATQNDAEKLVADLTAIVGSENVYNFFTDDSPIAEFVFASKERTQSRI
                     DSLNFLTDSTSSGILVASMAACRGLLPSPETYKGSKIQLEVGQEIEVDKLVKNLVNIG
                     YKKVSRVLTQGEFSQRGDILDIFDMQAETPYRIEFFGDEIDGIRIFDVDSQKSLENLD
                     EISISPASDIILSSEDYSRASQYIQTAIEQSTLEEQQSYLREVLADMQTEYRHPDLRK
                     FLSCIYEQSWTLLDYLPKSSPLFLDDFHKIADKQAQFEKEIADLLTDDLQKGKTVSSL
                     KYFASTYAELRKYKPATFFSSFQKGLGSVKFDALYQFTQHPMQEFFHQIPLLKDELTR
                     YAKSNNTVVIQASSDVSLQTLQKTLQEYDIHLPVHAADKLVEGQQQVTIGQLASGFHL
                     MDEKLVFITEKEIFNKKMKRKIRRTNISNAERIKDYSELAVGDYVVHHVHGIGQYLGI
                     ETIEISGIHRDYLTVQYQNSDRISIPVEQIDLLSKYLASDGKDPKVNKLNDGRFQRTK
                     QKVQKQVEDIADDLIKLYAERSQLKGFAFSPDDENQIEFDNYFTHVETDDQLRSIDEI
                     KQDMEKDSPMDRLLVGDVGFGKTEVAMRAAFKAVNDGKQVAILVPTTVLAQQHYANFQ
                     ERFAEFPVNVDVMSRFKTKAEQEKTLDKLKKGQVDILIGTHRLLSKDVVFADLGLLVI
                     DEEQRFGVKHKERLKELKKKIDVLTLTATPIPRTLQMSMLGIRDLSVIETPPTNRYPV
                     QTYVMETNPSVIRDAMLREIDRGGQVYYLYNKVDTIEQKVSELKELVPEATIGYVHGQ
                     MSEIQLENTLYAFVEGEYDILVTTTIIETGVDIPNANTLFIENADHMGLSTLYQLRGR
                     VGRSSRIAYAYLMYRPDKSLTEVAEKRLEAIKGFTELGSGFKIAMQDLSIRGAGNILG
                     AAQSGFIDSVGYEMYSQLLEQAILEKQGKATQRQKSNSEVNLQIDAYLPSDYIGDQRQ
                     KIEIYKRIKNIDSRVNYQELQEELIDRFGEYPDVVAYLLEIGLLKSFLDQVFCHTVLR
                     RQHQVTVTFEPMAGQIFLTQDYFEALSVTNLKAQITENKGKLAVVFNIQQKKEYEILE
                     ELISFAEKLKEIKARKAE"
     gene            7013..7285
                     /locus_tag="VCA20_001366"
     CDS             7013..7285
                     /locus_tag="VCA20_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEP1835178.1"
                     /translation="MRLDKYLKVSRIIKRRPLAKEVADKGRIKVNGILAKSSTDLKVN
                     DQVEIQFGNKLLTVKVLEMKDSTKKEDALKMYEIISEKRIEKDEEI"
     gene            7272..7640
                     /locus_tag="VCA20_001367"
     CDS             7272..7640
                     /locus_tag="VCA20_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEP1835179.1"
                     /translation="MKKSKILQLNNAFIQSERKKTQHQLAERQQKNRFMGAILILVIF
                     LFMLPAYNLVGTYTNIQQQEKKLAELEKNYEELTKEQKQEAEMVAKLKNEEYAAKYLR
                     AKYQYSKEGEFVYNIPGLPK"
     gene            7763..9043
                     /locus_tag="VCA20_001368"
     CDS             7763..9043
                     /locus_tag="VCA20_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEP1835180.1"
                     /translation="MQKKLLVWLLPVLFGWQVVDSTEIPFELTAQEEYELTHTIYDQY
                     FQTIPQNPNVFQTENLYSDEELTIAGGQLQPNQRFSITDVLVNSKKELVFKIDDKGYI
                     LASKHLLFDDVIVTETTVEQTYWTKKGFTLLTSPIANEATEIKNDLQPYQAVMVSKIV
                     TTSLGDFAYVTDKGWIAVNNLSKTDNRVEAVQELLTNKYSKDTIGIYVKQLSTGQTAG
                     VNQEKLFYSASIAKLPILYYVQEQLNAGDIDLTTKVKYTTESISFPGAYVAGGSGSLS
                     KTPDNKDYSVEELINKTAKESDNVASNLLSYYVANKFDSNFYQVITAKTGSEWSMVTR
                     ETSAEIAGKMMEALYIQNGYVLESLLSTQFDNQRISKDISVPVAHKIGDADDVKHDVA
                     IVYAGSPFVLSIFTDKSNYDEITQIANDIYGILK"
     gene            9044..10312
                     /gene="tilS"
                     /locus_tag="VCA20_001369"
     CDS             9044..10312
                     /gene="tilS"
                     /locus_tag="VCA20_001369"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEP1835181.1"
                     /translation="MKDRFLKVTLDGHFFDKHKKVLVAVSGGLDSMNLFHLLYECKQV
                     LGIELGIAHINHGQREESVIEEKYLRQLAEESNVPFYLSYFEGVFSEEAARKWRYGFF
                     ATIMEKEGYTALVTAHHADDQAETVFMRLIRGGRLRHLSAIQPTQPFATGELIRPLLS
                     FKKADFEKIFHFEDCSNADFKYFRNRVRNDYLPKLKQENPKIELTLNNLAVDTNNLFQ
                     ALRDLTQDLSVTDVMSFQQQTPAVQSYLLEEYFEKFPDLQLSRPQFNEVLHILRSKAN
                     YYHLLKNDYMLEKDYHRFQIYKIGPETDSQPEKIMIKSEGIFSYGSYIFSLNHPLEEA
                     DSILYFPTENPIVVRRRQAGDTILINGINKKLRRWFIDNKISQKVRQNALIIEQNGEI
                     YGITNLVSSDLSKSVKNDIIKATLYIKMKE"
     gene            10320..10862
                     /gene="hpt"
                     /locus_tag="VCA20_001370"
     CDS             10320..10862
                     /gene="hpt"
                     /locus_tag="VCA20_001370"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEP1835182.1"
                     /translation="MLDKDIKKILVSEEEIVAKCKELGQILATDYADKNPILVGILKG
                     SIPFMAELMKHIDAHVETDYMVVSSYHGGTESSGTVKIIKDLDNSVAGRHIIFVEDII
                     DTGRTLKELKELFALRQAASIKIATLLDKPEGRVVEIEPDYTCFTIPNEFVVGFGLDY
                     DENYRNLPYVGVLKEEVYTK"
     gene            10884..12857
                     /gene="ftsH"
                     /locus_tag="VCA20_001371"
     CDS             10884..12857
                     /gene="ftsH"
                     /locus_tag="VCA20_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEP1835183.1"
                     /translation="MNNQPNKGFIKNPFLIILVIATIVTAFQFFNAGQQVATQEISYS
                     QVVTELKNNNVSEITYQPNSSVIEITGKYKTEQEAKDELASTIKLFQVSSKVKYKNFK
                     SLILPSETNLSELQALANENGVKVTIKPESSNGLWLNIVFNLLPLIIAGVFFMMMMNQ
                     GGGGARGAMNFGRNKAKALEQSNIKVRFSDVAGAEEEKQELVEVVEFLKDPKRFTKLG
                     ARIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFED
                     AKKAAPAIIFIDEIDAVGRQRGVGMGGGNDEREQTLNQLLIEMDGFEGNEGIIVIAAT
                     NRSDVLDPALLRPGRFDRKVLVGRPDVKGREAILKVHAKNKPLAADVDLKLVAQQTPG
                     FVGADLENVLNEAALVAARRNKTVIDASDIDEAEDRVIAGPSKKDRQVSAKEREIVAY
                     HEAGHTIVGLVLSNAREVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDMKEQLAGLMG
                     GRVAEEIIFNTQTTGASNDFEQATQMARAMVAEYGMSEKMGPMQYEGSHAMSAGQTTQ
                     KHISEQTAYELDNEVRDLLNEARNKAAEIIQSNRETHKLIAEALLKYETLDSVQIKSL
                     YETGKMPENIERDDEDVHPLSYEEVKEKINTKE"
     gene            13209..13679
                     /locus_tag="VCA20_001372"
     CDS             13209..13679
                     /locus_tag="VCA20_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEP1835184.1"
                     /translation="MEFEKVYASVKGIVNKARKEFYIKLWDRDDWEQEGMMTLFELLE
                     AQPWLVDEQVQLYCYFKVKFRNRIKDRIRKQESQKRKFDRMPHEDIHELSHAIQSPGL
                     INDELLMLRGALRDYRKNLSNDQLDKYEKLISGQCFNGRREMIRDLQIHLKDFR"
BASE COUNT         4829 a         2379 c         2899 g         4055 t
ORIGIN      
        1 tagctaatcc taatgctggt agccatactg ctgaacatat catattcaaa ataaaagaaa
       61 gttatccaca gcttgcagtt aatattttta tgacagttgg tccttaggat gaaaaaagcc
      121 aaatagaggc tattttaaag gagtttgtca gtagtgagga ccaattaatg attttaggtg
      181 gagacggcac actgtctaaa gctttgcgtt tttggccagc tagtctaccg tttgcttatt
      241 atctaacagg atctggaaat gattttgcta aggcaatgaa tataacatcg ctatatagaa
      301 gtgtagatgc cattttagag ggaaaagcaa gtcggatata tgttttaaac agttcatacg
      361 gaacggttgt aaacagtatg gattttggct ttgcagctca agttatcaat ggttcaacga
      421 attcaatttt gaaaaaaatt ctgaacaagg taaaacttgg gaagttaact tatctattct
      481 ttggtattaa aacattattt tcaaaacaag ctataaactt agaattaact cttgatgaaa
      541 aatcttatca gttagataat ctctttttta tttctgtagc aaatagtctt tattttggtg
      601 gaggaatcat gatatggcca acagcaagtg ctaaaaagaa ggaagtagat attgtttact
      661 tcaaaaatgg aaatttctac caacgtctac aatcattgtt agccttatta acgaagaggc
      721 atgaatcttc tcacacaatt cagcatttaa caggggtaga tgtagtttta aaatcaaaag
      781 aaaaattatt attgcaaata gatggagaga catgcattgc aaatgaggta acgttaacct
      841 atcaggaaag aagtatgtat ctttaaggag gaagtatgta tcaattagga tcatttgtcg
      901 aaatgaaaaa acctcatgcc tgtgtcatca aatcgaccgg caagaaggcc aataagtggg
      961 aggttatccg tctaggagcg gatattaaaa tccgctgtag caactgtgac catgtcgtta
     1021 tgatgagccg gcatgatttt gaacgaaaaa tgcaacaagt gctgcctagt gaaacttagt
     1081 tgacagtttg caactgctgg ttgcgatttt ttcctaaaaa ttgctagtca actgttcttt
     1141 tctacactat aatagactta gaaaaaagag gaggaaatga tcatgaagca gttagcacag
     1201 caaattcgag ttttacgcac agctaagaac ctatcccagg atgacttagc agagaaactc
     1261 tatatttccc gtcaggctgt ttccaagtgg gaaaatggcg aagcaacgcc agatattgac
     1321 aaactagtac agctggcaga aatttttggt gtgagtctgg attatctggt tttaggaaaa
     1381 gagcctgaga aggaaattgt tgtggaacaa cgaggaaaaa tgaatgtttg ggaatacttg
     1441 aacgaagaat ccaaacgacc tcttacaaga ggagatgttg tcgttcttat ttttcttgca
     1501 gttatgcttt taggtggatt atttattcag cattattttt aatgaagctt gccaaacagc
     1561 aagctttttc tctacttttc tgctataata gtcatgattg aatttttaat tggagagtaa
     1621 aaaaacatgg ctttaacagc aggaatcgtc ggtttgccaa acgttggtaa atcaacccta
     1681 tttaacgcaa ttaccaaggc aggagcagag gcagccaact accctttcgc aaccattgat
     1741 ccaaacgttg gtatggtgga agtgcccgat gagcgtttgc agaaattgac agaactcatc
     1801 acccctaaaa agactgtccc aactaccttc gaatttaccg atattgccgg tatcgtaaaa
     1861 ggtgcttcta aaggtgaagg acttggaaat aaattcttgg ccaacatccg cgaggttgat
     1921 gccattgtcc acgtggtgcg tgcctttgat gatgaaaatg tcatgcgtga acaaggccgg
     1981 gaagacgcct ttgttgatcc tatcgcagat attgatacta ttaacctgga attgattttg
     2041 gcggacctag agagcattaa caaacgctat gcgcgtgtag aaaaaatggc ccgtacgcaa
     2101 aaagacaagg attctgtcgc agaatttgca gtccttgaaa aaatcaaacc tgtcttggaa
     2161 gacggaaaat ctgctcgtac agttgactta acagatgaag aacaaaaaat cgttaagcaa
     2221 ctcttcctct tgaccactaa accagtcctc tatgttgcca acgtcgatga agacaaggta
     2281 gcagatcctg agtctatcag ttatgttcag caaatccgtg actttgcagc aacagaaaat
     2341 gcagaagtgg tggtgatttc tgcgcgtgct gaagaagaaa tttcagaact cgacgatgaa
     2401 gacaaggacg agtttttaga agcactcgga ttaacagaat ctggcgtgga caaattaaca
     2461 cgtgcagctt atcacttgct tggtctaggc acctacttta cagcaggaga gaaggaggtt
     2521 cgtgcttgga ccttcaagcg aggtatgaaa gcccctcaat gtgctgggat catccactca
     2581 gacttcgaaa aaggctttat ccgtgccgta accatgtcct atgatgattt gattcaatac
     2641 ggctctgaga aggctgttaa agaagcagga cgccttcgtg aagaaggaaa agagtacgtg
     2701 gttcaagatg gggacatcat ggagttccgc tttaacgttt gatggctaaa ctgtccttat
     2761 tcttcgttgt tttaccttgc cataccatca gtattgtctg cggtttcaac gcctagacta
     2821 agaacagttt atcgcatcaa agaggatgat tacttggttg gaaagctttt tccagccttt
     2881 tggtatttaa ggagaaaaaa tgacacgatt gatcatcggt cttggaaatc ccggagaccg
     2941 ttattttgaa acaaaacata acgttggttt tatgctgctg gataagattg ctaagcgtga
     3001 aaatgtgacc tttaaccacg ataagatttt ccaagctgac attgccacaa cctttattga
     3061 cggcgaaaaa atctatctag taaagccaac gaccttcatg aacgaatcag gtaaagctgt
     3121 ccatgcctta atgacttatt atggtctgga tgcaactgat attttagtag cttatgacga
     3181 cttggacatg gctgtaggaa aaattcgttt tcgtcaaaaa ggttcagctg gtggccacaa
     3241 tggtatcaaa tccattgtca agcatattgg aaccaaggaa tttgaccgta tcaagattgg
     3301 tattgggcgt cctaaaggaa aaatgagcgt tgtaaaccat gtcttgtctg gctttgatat
     3361 agaggaccgt attgaaatcg acttagccct agataaactt gacaaggctg tcaacgtata
     3421 tctcgaagaa gatgattttg atacagtaat gagaaagttt aacggacaat gaatatactc
     3481 gatttacttc acaagaacaa gcaaatcaat cagtggcagt ctggcttaac caagtcaacc
     3541 cgccaactat tattaggact cactggaacc agtaagtcat tagtcatggc gactgcctat
     3601 gatagtatgg ctgagaaaat aatgattgtg actgctactc aaaatgatgc tgagaaatta
     3661 gttgcagatt taacagccat cgttggaagt gagaatgttt acaacttttt tacagatgat
     3721 agtcctattg ccgagtttgt ttttgcatca aaagaacgga ctcaatcaag gattgacagt
     3781 ttaaatttct tgacagattc tactagttca ggaattttag ttgctagtat ggctgcttgt
     3841 cggggcttat tgccgagtcc tgagacttat aaaggttcta aaatacagct tgaagttggt
     3901 caagaaatag aagttgacaa acttgtaaag aatcttgtca acatcggcta caaaaaagtg
     3961 tctcgtgtat taacccaggg agaatttagc caacgaggtg atattctaga tatttttgac
     4021 atgcaagcag aaactcctta ccgaatagaa ttttttggag atgagattga tggcattcgt
     4081 atctttgatg ttgacagtca aaaatcctta gaaaatctag acgaaatctc gatctctcca
     4141 gcttccgata tcattttgtc ttcagaagac tatagtagag ctagccaata cattcagaca
     4201 gctattgaac aatcgaccct agaggagcaa cagtcttact tacgagaagt cttagctgat
     4261 atgcagacgg agtaccggca tccagatttg cgtaaattct tgtcctgtat ctatgaacaa
     4321 tcatggacat tgttggatta cttgccaaag agttctccct tatttttgga tgattttcat
     4381 aaaatcgctg ataagcaggc tcaatttgaa aaagaaattg cagacttgtt gacagatgat
     4441 ttacagaagg gtaaaacagt gtcaagtttg aaatattttg cctcaactta cgctgaattg
     4501 agaaaataca aacctgccac cttcttttca agttttcaaa aaggtttagg cagtgtaaag
     4561 tttgatgctc tttatcaatt tacacagcat cctatgcagg aatttttcca ccagattcca
     4621 ctgttaaaag atgaattgac tcggtatgcg aaatcaaaca ataccgttgt tattcaagcg
     4681 agttctgatg taagtttaca gactttacag aaaactttac aagagtatga tatccatctt
     4741 ccagtacatg cagcagataa gttagtagaa ggtcagcaac aggttacgat tggtcagtta
     4801 gcttcaggtt ttcatttgat ggatgagaaa ctagttttca tcactgaaaa agagattttc
     4861 aataagaaaa tgaagcgtaa gatacgtaga accaatattt ctaacgctga acgtattaag
     4921 gactatagtg aattagcagt tggtgactat gttgttcacc atgttcatgg tatcggtcaa
     4981 tacctaggaa ttgagactat tgaaatttca ggcattcatc gtgattattt aacggttcaa
     5041 tatcaaaact ccgatcgcat ttctattcct gtagaacaaa ttgatcttct ctccaaatac
     5101 ttagcgtccg atgggaaaga tccaaaagtt aataaactaa acgatggccg gttccaacga
     5161 actaagcaaa aagttcagaa gcaagtggag gatattgcag atgatttgat taagctttat
     5221 gcggagcgga gtcagctgaa aggctttgcc ttttcaccag atgatgagaa tcagattgag
     5281 tttgacaact actttacaca tgtggaaacc gacgaccaac ttcgttccat tgatgaaatc
     5341 aaacaagata tggaaaaaga ctctccaatg gatcgtttgc tggtaggaga tgtcggcttt
     5401 ggtaagacag aggtagctat gcgcgctgct ttcaaagcag tcaatgatgg caagcaagta
     5461 gctattcttg ttcctacgac tgttttagcc cagcaacact atgctaattt ccaagaacgg
     5521 tttgcagaat ttcctgttaa tgttgatgtt atgagtcgtt ttaaaacaaa ggcagagcag
     5581 gaaaaaacac ttgataagtt gaaaaaaggc caagttgaca tcttgattgg tacccatcgc
     5641 cttctatcaa aagatgttgt ttttgctgat ttaggtttac tggttattga cgaagagcaa
     5701 cggtttggtg tcaagcataa ggaacgcttg aaggaattga aaaagaaaat agatgtccta
     5761 accctaactg caactccaat tcctcggaca ttgcaaatgt ccatgttggg aatccgcgac
     5821 ttgtcagtaa ttgagactcc accgacaaat cgctatcctg tacaaactta tgtgatggaa
     5881 acaaatcctt ccgttatccg agatgcaatg cttcgtgaga tagatcgcgg aggtcaggtt
     5941 tactatcttt acaataaagt tgataccatt gaacagaaag tgtctgaatt aaaagaattg
     6001 gttccagaag ctactatagg atacgtccat ggacaaatgt cggagatcca attagaaaat
     6061 actctctatg cctttgtaga gggtgagtat gatattctag tgaccacaac aatcattgaa
     6121 acaggtgttg atattccaaa tgctaataca ctgtttattg aaaatgcaga tcatatggga
     6181 ctgtcaactc tttatcaact tagaggacgt gttggccgtt ccagtcggat tgcctatgcc
     6241 tatctcatgt accgtccaga caagtccttg acagaagtag ctgaaaaacg cttggaagcc
     6301 attaagggct ttacagaact aggatcaggt ttcaagattg ctatgcaaga tttgtccatt
     6361 cgtggtgcgg gaaatatttt aggtgctgct cagtcaggtt ttattgattc tgtgggatat
     6421 gagatgtatt cacaattgct ggaacaagcc atcttggaaa aacaaggtaa ggcaacccaa
     6481 cgtcagaaga gtaattcaga ggtcaattta cagattgatg cctatttacc aagtgattat
     6541 attggtgatc agcgacaaaa aattgaaatt tacaagcgta taaagaatat tgacagtcgt
     6601 gtaaactatc aagaattaca ggaagaattg attgaccgtt ttggagagta tcctgatgta
     6661 gtagcctact tacttgaaat cggtctattg aagtcatttt tagaccaagt tttctgtcat
     6721 acagttctta gacgacaaca tcaagtgaca gtaacattcg aacctatggc tggtcaaatt
     6781 ttcctaacgc aagattactt tgaagcttta tctgtaacaa atttgaaagc tcagataacg
     6841 gaaaataaag gaaaactagc tgttgtgttt aacattcaac agaaaaaaga atatgaaata
     6901 ttagaggaat tgatttcttt tgccgaaaaa ttaaaagaaa ttaaagcaag gaaggcagaa
     6961 taaatttctc aaatacctta aaaaatggta aaataatgaa ctgaggtaaa gtatgagact
     7021 agataaatat ttaaaagtat cccgcattat taagcgtcga ccccttgcga aggaagttgc
     7081 cgataaagga cgaataaaag tcaatgggat tttggcgaaa tcttcaacag atttaaaagt
     7141 aaatgatcag gttgaaatcc aatttggtaa taaactactc actgtaaaag tacttgaaat
     7201 gaaagattcg actaaaaaag aagacgcact gaaaatgtat gaaattatca gtgaaaaaag
     7261 gatagaaaaa gatgaagaaa tctaaaattc tacaactgaa taatgccttt attcagtcgg
     7321 agcgtaaaaa aactcagcat caattagcag aacgccaaca aaaaaatcgt tttatgggtg
     7381 caattctcat tctagttatc tttcttttta tgttgcctgc ctataatctt gttgggactt
     7441 acactaatat tcagcagcag gaaaagaaac ttgctgaatt ggaaaaaaat tacgaagaac
     7501 tgaccaaaga acagaagcag gaagcagaga tggttgcaaa attgaaaaat gaggaatacg
     7561 cagcaaagta tcttcgggct aagtatcaat actctaaaga aggggaattt gtctacaata
     7621 ttccagggtt accaaaatga ccgaatctat ttttaaaatt gtagagcaat ttttacagca
     7681 ttctgatgaa aaattagaag agttgaatca aaagaataga gaattaaagt tagaagaaaa
     7741 tgaatcgtag aaaggaggga gtatgcagaa gaaactgctc gtatggttat tgccagtttt
     7801 atttggatgg caggtggtcg atagtacaga aataccattt gaactcacag cgcaagaaga
     7861 atacgaattg actcatacta tctatgatca gtattttcaa actatccctc aaaacccaaa
     7921 tgtttttcaa actgagaatc tttattcgga tgaagaactg actatagcag gtggacaatt
     7981 acagccgaac caacgttttt ctattacgga tgttctagtc aatagcaaaa aagaattggt
     8041 tttcaaaata gatgacaagg gttacattct tgcaagtaag catctactat ttgatgatgt
     8101 aattgttaca gaaactactg tagagcaaac ttattggaca aaaaaaggtt tcacattgtt
     8161 aacctcacca attgctaacg aagctacgga aataaaaaat gatttacaac cctatcaagc
     8221 tgttatggtt tctaaaatcg tgacaacttc cctaggtgat tttgcctatg ttacagataa
     8281 aggttggatt gcagtaaata atctttctaa aacggataat agagtagaag ccgttcaaga
     8341 gttgttgact aacaaatatt ccaaagatac tattggtata tatgtgaaac aattgtcaac
     8401 aggtcaaact gcaggtgtaa atcaagagaa actattctat tctgccagca ttgctaaact
     8461 accaattctt tattacgttc aagagcaact gaatgctggg gatattgatt tgactactaa
     8521 agtgaaatat acaaccgaat ctatatcctt cccaggagct tacgttgctg gaggaagtgg
     8581 ttccctgtca aaaactcctg acaataaaga ttattcagtg gaagaattaa tcaataagac
     8641 tgctaaagag tcagataatg tagctagtaa tttactttct tattatgtag ctaataaatt
     8701 cgattccaat ttttatcaag taataactgc taaaacaggt agtgagtgga gtatggttac
     8761 acgtgaaact tcagctgaaa tcgcaggtaa aatgatggaa gccttatata ttcaaaatgg
     8821 ctatgtgcta gaaagtttgt tatctactca atttgataat caacgtattt cgaaagatat
     8881 ctcagttcca gttgctcata aaattggcga tgcagacgat gtcaaacacg atgtagcgat
     8941 tgtttacgca gggtctcctt ttgttttatc tatttttaca gataaatcaa attatgatga
     9001 gattacacaa attgcaaatg atatttacgg gattttaaag taaatgaaag atagattttt
     9061 aaaagtgaca ctggatggtc acttttttga taaacataag aaagtactag tagccgtatc
     9121 tggtgggtta gattctatga acctattcca tcttttatac gaatgcaaac aagtgttagg
     9181 gattgaactt ggaattgccc atattaatca tggacagaga gaagaatctg tcattgagga
     9241 aaaatatctt agacagttag cagaagaaag taatgttccc ttttatctgt cctattttga
     9301 aggtgttttt tcagaagaag cggcgcgaaa atggcgttat ggattttttg ctacaatcat
     9361 ggagaaggaa ggctacactg cactagtgac agcccaccat gcagatgatc aagcagaaac
     9421 tgtctttatg cgattgatta gaggagggcg tttacgtcac ttatcagcaa ttcaaccaac
     9481 tcagcctttt gcaacagggg aacttattcg accactttta tcttttaaaa aggcagattt
     9541 tgaaaagata tttcattttg aagattgcag caatgcagat ttcaaatatt ttcgaaatag
     9601 agtcagaaat gactatttac caaagttaaa acaggaaaat cctaaaatcg aacttactct
     9661 caataatctt gcagttgata caaataatct atttcaagcg ctaagagatt tgacacagga
     9721 tttatccgtt acagatgtga tgagttttca acaacagaca cctgcggtac aaagttatct
     9781 attagaggaa tattttgaaa aatttcctga tttacagctc tctcgtccac aatttaatga
     9841 agtattacac atcttgcgtt caaaagctaa ctactatcat ttattgaaaa atgattacat
     9901 gctagaaaag gattatcatc gttttcaaat ttataaaata ggaccagaga cggatagtca
     9961 accagaaaaa atcatgataa aatcagaggg tattttttct tacggttcat atattttttc
    10021 tctcaatcat cctttggaag aagcggatag tattttatat tttcctacag aaaatccaat
    10081 tgtagtaagg agaaggcagg ctggggatac cattttaata aacggaataa ataaaaaact
    10141 ccgccgttgg tttattgata ataaaatctc acagaaagtt cgacaaaatg cgcttattat
    10201 cgagcagaat ggagaaattt atggaattac gaatcttgtt agcagtgatt tgagtaaatc
    10261 agtaaaaaat gatataatca aagctacctt atatataaaa atgaaagagt agaacaacta
    10321 tgttggacaa agatattaag aaaattcttg tttcagaaga agaaatcgtt gcaaaatgca
    10381 aagaactcgg tcaaattcta gcaacagact atgcagataa gaatcctatt cttgtaggaa
    10441 ttttaaaagg ttctatccca tttatggcag aattaatgaa acatattgat gcccatgtag
    10501 aaacggatta tatggttgtt tctagctacc atggcggaac ggagagtagc ggtaccgtta
    10561 agattatcaa ggatttggac aatagtgtag caggtcgaca tatcatcttc gtagaggata
    10621 ttattgacac tggtcgcaca ttaaaagaat tgaaagaact ctttgctttg cgtcaggcag
    10681 cttctataaa aattgccact cttcttgata aaccagaagg tcgtgttgtt gaaatcgagc
    10741 cagactacac ttgctttact attccaaatg aatttgtagt tggatttggc ttagattatg
    10801 atgaaaatta tcgtaatctt ccttatgttg gtgtactcaa agaggaagtt tacacaaaat
    10861 agaaaaaggt ttcctactat attatgaaca atcaaccaaa taaaggattt ataaaaaatc
    10921 cttttctcat tattctcgtt attgccacta ttgttacagc ctttcaattt tttaacgctg
    10981 gtcagcaagt agcaactcaa gaaatcagtt attcacaagt agtaactgaa ttgaaaaata
    11041 ataatgtatc tgaaattact tatcaaccaa attcaagtgt tattgaaatt actggtaagt
    11101 acaaaacaga acaggaagct aaagatgagt tagcttcaac tattaagtta ttccaagttt
    11161 ctagtaaagt taagtataaa aactttaaat cactaatttt accgtcagaa acgaatttgt
    11221 cagagctaca agctcttgca aatgaaaatg gagtaaaggt tactattaaa cctgaaagtt
    11281 caaatggact ttggttaaat atcgtcttta atttgttacc gcttatcatt gctggtgtat
    11341 tcttcatgat gatgatgaac cagggtggtg gcggagctcg aggtgccatg aactttgggc
    11401 gaaacaaggc aaaagctctt gagcaaagca atatcaaagt tcgtttctca gatgttgcag
    11461 gtgcagaaga agaaaaacaa gaattggtag aagtggtaga atttttgaaa gatcctaaac
    11521 gctttaccaa acttggtgcc cgtattccag caggtgtctt attagaggga cctccaggaa
    11581 caggtaaaac cttgcttgcc aaagcagtag cgggtgaagc gggtgtacca ttcttctcca
    11641 tttctggttc agattttgtt gaaatgtttg ttggtgtcgg tgccagccgt gttcgttcat
    11701 tatttgaaga tgcaaaaaaa gcagcaccag ccattatttt catcgatgaa atcgatgctg
    11761 tcggtcgcca acgtggtgtc ggcatgggtg gcggtaacga cgaacgtgaa caaaccctca
    11821 accaactctt aattgaaatg gatggttttg aagggaacga agggattatt gtcattgccg
    11881 caaccaaccg tagtgatgtt ttagaccctg cccttcttcg tccgggtcgt tttgaccgta
    11941 aagtattggt tggtcgtccg gatgttaaag gacgtgaagc aatccttaaa gttcatgcga
    12001 agaataaacc actagcagca gatgtagatt tgaaattagt agcacaacaa acaccaggat
    12061 ttgttggtgc agatttagag aatgttctta acgaagcagc ccttgtagct gcacgtcgaa
    12121 acaagactgt cattgatgct tcagatattg acgaagcaga agatcgtgtc attgcaggtc
    12181 catctaagaa agaccgtcag gtttcagcca aagaacgcga aatcgttgcc taccatgaag
    12241 caggtcatac aattgttggt ctcgtactat caaatgcacg tgaagttcac aaagtaacaa
    12301 ttgttccccg cggtcgcgca ggtggataca tgattgccct tcctaaagaa gatcaaatgc
    12361 ttctttctaa agaagatatg aaggagcaat tggctgggct tatgggtggt cgtgtagcag
    12421 aagaaattat ctttaatact caaaccacag gtgcctcaaa tgactttgag caagctacac
    12481 agatggcgcg tgctatggtt gcagaatatg gtatgagtga aaaaatggga ccaatgcagt
    12541 atgagggcag tcatgcaatg tctgctggac aaacaactca aaaacatatt tctgagcaaa
    12601 ctgcatatga gttagataat gaagtacgtg atttgttaaa tgaagctcgc aacaaagcgg
    12661 ctgaaattat tcagtctaat cgcgaaactc ataaactaat cgcagaagca ttgctcaaat
    12721 acgaaaccct agacagtgtt cagattaaat ctctctatga aacaggcaaa atgccagaga
    12781 atattgaacg tgatgatgaa gatgttcatc ccctttctta tgaagaggta aaagagaaaa
    12841 ttaatacgaa agagtagtat aaaaatactc tgggcaagac attccataac ggatctgccc
    12901 tcactctcaa ccactaaaat aaccacaaag ccttcgctaa gcacataatc ttggcgaggg
    12961 cttttttaca tgatacatat cagaaacagg gcatttacaa gaaaaacgaa tcaatagtga
    13021 tttatctcaa tcttagaact ataccaaata actcccttcc gaagcagaac gcaatgtttg
    13081 tcatagagta tcaatagcaa atgtacttta aaaaagaaaa atcaaataaa gtatgctaga
    13141 caaaaatgtc ctgtagaaag ccagcacttt tcgataaact taaaccatca acaaaaggag
    13201 attaagtaat ggaattcgaa aaagtgtacg caagcgtcaa aggtattgta aacaaggctc
    13261 gaaaagagtt ttacattaaa ctatgggatc gagatgattg ggaacaagaa ggaatgatga
    13321 ccttgtttga attgttggaa gctcaaccgt ggctagttga tgaacaagtt caattatatt
    13381 gttattttaa agtcaagttc agaaatcgaa tcaaggatcg tatccgcaaa caggaaagtc
    13441 aaaaacgcaa gtttgaccgt atgccacatg aagatattca cgaattatct cacgcaatac
    13501 aatcaccggg attgataaac gatgaactat taatgctaag aggtgccttg agagattatc
    13561 gaaaaaatct gagcaatgat caacttgata aatatgaaaa attaattagt ggacaatgtt
    13621 ttaatggtcg ccgtgaaatg atacgtgatt tacaaattca tttgaaagac tttcgctaaa
    13681 gctagttact aacatcacgt taatcgaatt aagaaattgg gcagaagcac cataaaaagt
    13741 tctgttcagt ttctttttag ataggttata gcacaggaag aaggaatagg agaaaaaaca
    13801 aagtatctac atagaacttt cagtgtaaaa aatctcaaaa aaccggttga caatgtatat
    13861 aagtcgtgat agactaaatg agttgtctct tgaagggcta gttaatagag gaacgaaaaa
    13921 agtttcgaaa aagtgttgac aagaccatca gaagatgata gaataattga gttgtctctt
    13981 gagggactag ttaacagagg aacgaaaaaa agtttcaaaa aagtgttgac aaagttcaca
    14041 agaaatgata aactaagata gttgtcgcga gagcgcgata acgacaagac ctttgaaaat
    14101 taaagaagac gaaccaaacg tgcagggtga tttatctaag gataaatcgt caatgacaaa
    14161 ac
//
LOCUS       DATUCG010000059        14019 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.060, whole genome shotgun sequence.
ACCESSION   DATUCG010000059 DATUCG010000000
VERSION     DATUCG010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14019
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.060"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            324..911
                     /locus_tag="VCA20_001373"
     CDS             324..911
                     /locus_tag="VCA20_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HEP1835185.1"
                     /translation="MDWLKELIEKHTAEGKTDIDAVMNAVKEEFPKHAVPKDVYNEQA
                     EKLKVANSTLDTLKKSNKDNEELQNELKTYKDKVSQLEAEAKETAKKQTIKDALINAK
                     ATDVDYLMYKLGNLEEDDSGKIKDLDQKIKDLQVNLPNFFESAEAKEDKLNGYEHLGG
                     AGLLPGSAESTKDVIDVIGNKELNLTQFLQEKAKG"
     gene            918..1907
                     /locus_tag="VCA20_001374"
     CDS             918..1907
                     /locus_tag="VCA20_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coat protein"
                     /protein_id="HEP1835186.1"
                     /translation="MSNEITKIIDTITPKVFNAYMDQYINEHSLLVRSGAVVADERVS
                     RNITAGGLTVNMPFWNDLSGEDQVLSEDKEIETGKITSGKDVAAVLYRGQGWSVNELA
                     GVVSGSDPMRAILDRIGGFWLRQEQKVLMAVLKGLFATSGPLADTHLLDLSKKTGKAA
                     VVDAKAILDAKQLMGDASRKLKILAVHSSVYTKLQKDNLIQYIQATDVNIQIPTYLGY
                     TLLVDDALAPEGDVYTSYLLGSGAFGRNTGHPEGLTTFETERKAAAGVDNVYTRRALT
                     MHPYGVRWQDNSIVGLTPSNAELATAENWNKVYEDKNIGIVAIRHKLAFSSEV"
     gene            1910..2236
                     /locus_tag="VCA20_001375"
     CDS             1910..2236
                     /locus_tag="VCA20_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835187.1"
                     /translation="MVKYITIDLFTDTDDERVEYPIGALYPREGYTPSKERIDSLLSG
                     DNARGVPVIKQIIELPAKTTIPSDSENELTRDAIKEKLTQLGIEFKAKAKTESLLELL
                     KTGEEE"
     gene            2271..2801
                     /locus_tag="VCA20_001376"
     CDS             2271..2801
                     /locus_tag="VCA20_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835188.1"
                     /translation="MIISLNEALIIHPKATQKMCDGLETMVRKVTNNHFQLEKFRLTH
                     LSLEGSTIKVDKGRLDIFREGDTVEINGTDFNDGLYVISGVSDGILSVNSTFIPEYKK
                     GAILTKISYPTDVIEGVKKLLAYDAKMTDKIGVKSESVARWSVTYYDVTASESQEGYA
                     AVLLGFLSKYKKIRWS"
     gene            2804..3103
                     /locus_tag="VCA20_001377"
     CDS             2804..3103
                     /locus_tag="VCA20_001377"
                     /inference="COORDINATES: protein motif:HMM:TIGR01563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEP1835189.1"
                     /translation="MEVTLLKCQASRKNALGQKVYDFVESEKLEGFLDMLTGNEADRA
                     TALTSSTHSFLTKQVDVQITTKDRIRAKGIDYEVSFVDNPVNLDHHLEIYLKVVA"
     gene            3103..3507
                     /locus_tag="VCA20_001378"
     CDS             3103..3507
                     /locus_tag="VCA20_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEP1835190.1"
                     /translation="MKFIDNSAKAKEEIKYQAIRALKEICMMVEAQAILLAPSDTGNL
                     REHISHTVDRHELIGYVGTNVSYAIYVEFGTGEFAEKGNGRKGGWLYTTPDGKSYFTR
                     GNKPQPYLRPAFRKNKKQIQAIFETYLSQIGG"
     gene            3509..3877
                     /locus_tag="VCA20_001379"
     CDS             3509..3877
                     /locus_tag="VCA20_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835191.1"
                     /translation="MTPVIAKLTELFTEIVPEAYFLSNSNPTVAYPYLIFSYDAENRH
                     WGQDGAYVDVDIFDNKGEDQEQIEHLAFLLKRELEHRAVMLEECYIRFRFEGQGTIDT
                     MSDVLQRRNVRFYITIDWRN"
     gene            3934..4488
                     /locus_tag="VCA20_001380"
     CDS             3934..4488
                     /locus_tag="VCA20_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835192.1"
                     /translation="MVNAGAVYKNLTWNTETQKWEGELFGATSGGNKLTIEQNYRQIE
                     VDGVFTAAVGQKVLESQGAKLEINAKELTAENIRMAINGVIEQGDGQKVPATSKVITA
                     KGELKKTDYITNLGLVGNLSGTNEPIIVILDNALCVSGLEFETKDNDEAVITMTFEAH
                     ANEEQIDDLTLPCRIIYPTVDMEV"
     gene            4490..4912
                     /locus_tag="VCA20_001381"
     CDS             4490..4912
                     /locus_tag="VCA20_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835193.1"
                     /translation="MAEKLEMRELNGGDIFTMLGIIGKLDIKDEVVELLERQFNQPAI
                     VLQDHKKKQPTKAEKEAAELATEKRGMLLMTDIGFAILRHVGEAKNDVNSFLADLTGT
                     SRKDIESLSMLAYTGLLMDFAKKAELRDFFQSISSVLA"
     gene            5008..5238
                     /locus_tag="VCA20_001382"
     CDS             5008..5238
                     /locus_tag="VCA20_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835194.1"
                     /translation="MYLFDEEDKDVLKEIWLAKEVDMSLQSFIDKHSKKRYISKVNRK
                     QILESDTDSALKNAESILNLGYGKEVRLDGNL"
     gene            5225..8305
                     /locus_tag="VCA20_001383"
     CDS             5225..8305
                     /locus_tag="VCA20_001383"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PblA"
                     /protein_id="HEP1835195.1"
                     /translation="MEIFKLFGSIGLKNKEANQAIDETTGKARTAGDKIKDTFGKVAT
                     FLGTVFAGKAIFDFGKSVVEAAATAKAVQAQFDQVFEGIRDVAENKLNDLAKTVGAVP
                     SRIKPAFNQIASFAKVAGMDASQSLEFTTRATEAAADSAAFYDKSLEETTETLKSYLK
                     GNFNVADNLGILSTETTRNAKATELFGQKYKDLDGIQQQEVLLRMFEDANKVSGAMGQ
                     AARESDGFENVMGNLKQTWEDFKVLVGGPLLQPVVAGLQLATKWIQDLGGKFQEFEPK
                     ITLFKELIASVYDVIFGSLSVKDNVDLLVKLGMDEESVNAILSTASLIGAALTSFYDI
                     IFGSLSKKDNIDFMESMGIDKSTAVTIADIAENIRVSFENIGQVVSDAISIVGDFIFE
                     LLGVAGSETSVNIVAEAFKTVTGFLRDASEKLKDFTGFLKSNPAVLDGLKSAVVGLTA
                     AWTSYKVITGVLKGIETARNTLLAVGNGLMLARFTQSGALTVAEATQAAATMGATGAF
                     SAFNAVLSLNPIMLVVGAIAALVAGLVWFFTQTETGREMWSNFIEWAQNAWEGIGEFF
                     SNLWQSITEGAINLWNGIVDTWNHAVEIVKNVWSSISEFFTNLWADVQTTAIEAWNTF
                     VQGVMMVVQPFIDTFMQYWTTMSDGLSQMWEGVKMFFQGAWEFIKAIFMGAVLIILDI
                     VTGNFGRLGADLDLIWESIKSAITMVWDGIKTYFSGVIDAIVAFGTTYFENFKNLLSA
                     IWEGVKSAAQGAWDWIKTTVSNLITSLIDWARSTWDGFKNFLSSLWEGIKSTATNAWE
                     NLKQGVQNIIDGLVSGAKRAWDNLKQSVSDLVSSVTSIFDNLRNIDLFAAGQAILDGF
                     LGGLKSAWNNVTGFISGIADWIFENKGPIEYDRKLLIPAGNAIMEGLGGGLEDGFDDV
                     QDGVLGMAGKLVQKVSSAAKHFKDTVVPQMGDWSSSLDWYAKGEIISLPSMYDVSSSN
                     LLEHSSDGQSNSLLQKIIEILQALLDKDSDVYLDLEKVGRMTYDEHGRIIERGG"
     gene            8309..9004
                     /locus_tag="VCA20_001384"
     CDS             8309..9004
                     /locus_tag="VCA20_001384"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835196.1"
                     /translation="MLELKVDGISTKTIVGCIVAGLGEDRASSPRFIEETIYGMNGTN
                     RTIEAYDEVDRVLTFHCASLDAVRQLIKLFKGLDKKLEFWHIPNSFYYFDYKSSSYKI
                     NHKCSWDVSVKISLMPFRYLSEVEDIVLTQSGAITNMGDIFSEPRIEVFGNGQTSLTI
                     GNQVLRLNLDTKAVIECRHGYQNVYDKNGIIKNSIRTSGPFFEIPAQVTSGVVLGSGI
                     NRVVIRPRWRCVI"
     gene            9868..12696
                     /locus_tag="VCA20_001385"
     CDS             9868..12696
                     /locus_tag="VCA20_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835197.1"
                     /translation="MKITSYRFSPMGRKLKSIGFGKIKTSLTQQVSNIATEVAEQSVS
                     VAGQVFEEKLQKEIENADKVIDGKINKVRDEINDGLEQSKAEAEAYADNIKQRIDGQL
                     AESDRQYQLAQQTQGRQIADVLAKATAAKEIAEQTVTDLMRVQNAFNQSIGQANTKAL
                     ELERSIGTVRTDVMSQAQTILAQAQTQTELTNRVTTVETLADGTKTTVAELSKTVSKA
                     TGDITSVTSRTKTVEETLSQTRTQYEALTQTVNTQTGQIESINRKTADLQSGIDGVTE
                     RFESLRVGATNYIRDFGFAKNHNFSSYQSIWQFERISDSTARSGYLVKATCTQAGGAG
                     FYRRFFDLRGEEWQGRTMTYAVDIKASKSVRMRLGAEAFNKSYRAFDVTTDWQRFVST
                     DTVNYKTDYGFTFYIDGAQWQVGDIVYIRDPQLEDGSIATTPHASEEDLRSEFATYVR
                     NAAENSAELVRRIETADGKAVDVKTYAQQTATAINTRLESLETYKNAEGTRASQYFSA
                     SRDETARKVTALRTAVTDGYVAKAKYEEDARGVMQRFESLSVDQQSYTDIKIAEYKTT
                     VDGRFATMQTSIDSKASQVAFQRVQETSQLYERIIGSTETGIKDKVARMVMADSLFLT
                     EVKDKISGTATQVSQLNNSYAIKNLTSAGTVLNQINLLANGTNRIDGRLTHITGQTLI
                     DNGVIKNAMIGNLDAGKINTGTLNAARIATNSIDGSKLVFDQAFVNKMTANEALFKQL
                     FAKNAFITSVQAVTLSASQITGGLMRATNRAMEVNLNAGQILYYTDQAALKRVLNGYP
                     TQFVKFATGTVTGKGNAGVTVIGSNRWNSESSNDGGFVGIRAWNGPNIDQIDVVGDTV
                     RLASSAFEAADGWNINTLPGKLDIDAFNADHRASSKIKVGDLWLWKNATTYSSMRDTI
                     NLIIDNLQLLHNNKSTERGYSYTLPAKV"
     gene            12711..12923
                     /locus_tag="VCA20_001386"
     CDS             12711..12923
                     /locus_tag="VCA20_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835198.1"
                     /translation="MNQEQLNQALALTNQELANQLAEEKNTKNLLAVQLTEAQQTIAS
                     LQTEIKELTQQLDEATKPAEIIEEGE"
     gene            12926..13171
                     /locus_tag="VCA20_001387"
     CDS             12926..13171
                     /locus_tag="VCA20_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835199.1"
                     /translation="MPLETIKTTQLIGNLKIGDEIVKTYTVNIDNKGVSRIFETVYNQ
                     ELYAANRKEMRKQEAEFREKRYEVEDAILAELEPKEE"
     gene            13175..13510
                     /locus_tag="VCA20_001388"
     CDS             13175..13510
                     /locus_tag="VCA20_001388"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835200.1"
                     /translation="MLGQDIVREAIKASWTIDKIAGVVVVILAIMLVSMVVSQNANVK
                     RLINNFQKTNDALMETNRRIASDNQRHMEHLTTAVNNLASETRNDIADLKEQVSELKE
                     EVRDQRMMP"
     gene            13521..13850
                     /locus_tag="VCA20_001389"
     CDS             13521..13850
                     /locus_tag="VCA20_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEP1835201.1"
                     /translation="MEHLTELILGTATGIFGIVGGMIVHEVKKYLIAKGGKRAVEITE
                     ILARNAVNAVEQITKLDQDKHVDKLDMAKRRVTGQLAKYNIYMTDTQLETFIESAVKQ
                     MNDAWKE"
     gene            13853..>14019
                     /locus_tag="VCA20_001390"
     CDS             13853..>14019
                     /locus_tag="VCA20_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HEP1835202.1"
                     /translation="MVINLETSIRWMSDRVGKVSYSTDYRNGPNSYDCSSAVYYALMA
                     GGAISAGWAVNT"
BASE COUNT         4448 a         2532 c         3348 g         3691 t
ORIGIN      
        1 agctaaatga aattgccgag ctacgttcaa tcatcgaacg caacgcccag gctacgaatt
       61 cgagatttga caaaatcgag aagcaagtag ctaatagtaa tgctaagaag ccgttttgga
      121 aacggtaagg aggtggttca acatcttgac agcaggaaag actgcaataa ttacatagcc
      181 taaccgtgtc gaattcgatg cggttttctt tatgtcctgt cgcatgacgg aaaactaggc
      241 aactacccaa attgtcgcag ggtatattgt gacaactccc acagggggag agcccctaat
      301 aaaatctatg gaggtaacca ataatggatt ggttgaaaga acttatcgag aaacatactg
      361 cagagggtaa aacagacatt gatgcggtca tgaacgcagt caaagaagag ttcccgaaac
      421 acgctgtccc gaaagatgtc tacaacgagc aggctgagaa gctaaaagtc gctaacagca
      481 cgctagacac cttgaagaaa tcaaacaagg acaacgaaga gctgcagaat gaactcaaaa
      541 cgtacaagga caaggtgtcg cagctagaag ccgaggcaaa ggaaacagcc aaaaaacaga
      601 caattaagga cgcgctgata aatgctaagg cgactgatgt agactacctc atgtacaagc
      661 ttggcaactt ggaggaagac gatagcggaa aaattaagga tttagaccaa aaaatcaaag
      721 atttacaagt caacttgccg aacttttttg aatctgctga agcaaaagag gataagctga
      781 atggctatga acatttgggc ggtgcgggtc tcttacctgg ttctgcagag tctacaaaag
      841 atgttatcga cgttattgga aacaaagaat tgaacttaac tcaattctta caggaaaaag
      901 cgaaaggata gaaaaatatg tctaatgaaa ttacaaaaat tattgatacg atcactccaa
      961 aggtatttaa tgcttacatg gaccagtata tcaacgaaca ttcgttgcta gttcgctctg
     1021 gtgcggttgt cgctgacgaa cgtgtatctc gaaatattac ggctggtggt ctaaccgtta
     1081 acatgccgtt ttggaacgat ttgagcggag aggaccaagt cttatccgaa gacaaagaaa
     1141 tcgaaacagg aaaaattact tctggaaaag atgtagcagc tgttctttac cgcggacaag
     1201 gttggtcagt aaacgaattg gctggtgttg tctctggttc agaccctatg cgcgctatct
     1261 tagatcgcat tggtggattc tggcttcgtc aagaacagaa agtgctaatg gcggtactga
     1321 aagggttgtt tgctacgagc ggacctcttg cagatactca tttgttggac ttaagtaaaa
     1381 aaactggaaa agcagcagtt gtggatgcta aggctatctt agacgctaag cagttgatgg
     1441 gtgatgcttc tcgcaaactc aaaattttgg cagtacattc ttctgtttat actaaattgc
     1501 aaaaggataa cttaattcaa tacatccaag ctaccgatgt aaatattcag attccgactt
     1561 atttgggtta tacattgcta gttgatgatg cattggcccc agaaggtgac gtttacacat
     1621 catatttgct aggcagtggt gcttttggac gcaatacagg tcatccagaa ggtttgacaa
     1681 cattcgagac agaacgcaag gctgccgcag gggtagacaa tgtctatact cgtcgcgctc
     1741 taacaatgca cccgtatggt gttcgctggc aagataacag cattgtagga ttgactccat
     1801 caaacgcaga attggcaact gccgaaaact ggaataaagt ctatgaagac aagaacatcg
     1861 gtattgtggc tattcgtcac aaattagctt tttctagcga ggtgtaagca tggttaagta
     1921 tattacaatt gatttgttca ctgatacaga tgacgagcgt gtggagtatc ctattggtgc
     1981 tctctatcct cgtgaagggt atactccctc aaaggagcgt atcgactctc ttttgagcgg
     2041 tgacaatgct aggggtgtac cggtgattaa gcaaattatc gaattaccag caaaaacaac
     2101 tattccatct gactctgaaa acgaattgac tcgagatgcc atcaaggaga aattaaccca
     2161 gctcggcatt gagtttaaag ccaaggctaa gacagagagt ttgctggaat tgttgaagac
     2221 aggcgaagag gagtagttga actccttttt tctttttgag gaggttctat atgattatat
     2281 ctttaaatga ggctttaatt attcatccga aagcaacgca gaaaatgtgt gacggactag
     2341 aaactatggt tcgcaaggtg actaataacc attttcagct agaaaagttc cgcttgacgc
     2401 atttgtcgtt ggaaggttcg actattaaag tcgataaagg acgtctggat atttttcgag
     2461 agggagacac agtcgaaatt aacggtactg attttaatga cggtctatat gttatttccg
     2521 gcgtctctga cggcattttg tctgtcaata gtacatttat accggaatat aagaaagggg
     2581 ctattttaac gaaaataagc taccctacag acgtgattga aggtgtaaag aaactactag
     2641 catatgatgc taaaatgaca gataaaatcg gagtgaagtc tgaatctgtc gcacgttggt
     2701 ctgtaactta ctacgacgtg acggcttccg agagtcaaga aggatatgcc gcagttttgc
     2761 taggtttttt atcaaaatac aaaaagataa ggtggtcttg acgatggaag tgaccttact
     2821 taagtgccag gcaagcagaa aaaacgccct aggtcaaaaa gtttacgatt ttgtggaatc
     2881 tgaaaagctg gaaggcttct tagatatgct tacgggtaac gaagcggata gagcgacagc
     2941 tttaacatct agtactcaca gctttttgac gaagcaggta gatgtccaaa ttacaacaaa
     3001 ggatagaata agggccaaag gaattgatta cgaagtttca tttgtagaca atccagtcaa
     3061 tcttgatcac cacctggaaa tttatctgaa ggttgttgct tgatgaaatt tatagacaat
     3121 agcgccaaag ctaaggaaga gattaaatat caagccattc gtgctctgaa agaaatttgc
     3181 atgatggtag aggcgcaagc gattctactt gctccttcgg ataccggaaa tcttagagag
     3241 catatcagtc acacggttga taggcacgaa ttgattggct atgtagggac taatgttagt
     3301 tatgccattt atgttgaatt tggtacgggt gagtttgcag aaaaaggaaa cggacgcaag
     3361 gggggatggc tatatacaac ccctgacggg aagtcgtatt tcactcgtgg gaataaacct
     3421 caaccttatc tacgacctgc ttttcgtaaa aacaaaaaac agatacaggc gatttttgaa
     3481 acgtatctga gtcagattgg aggataaaat gactcctgta attgctaaat tgacagaatt
     3541 atttaccgaa attgttcctg aagcctattt cttatcaaac tcaaacccaa ctgttgcgta
     3601 tccctacctc atattttctt atgatgcaga aaatagacat tggggtcaag atggggctta
     3661 tgttgatgtc gatatttttg acaataaagg cgaggatcaa gagcagattg agcatctagc
     3721 ttttttatta aagcgagagc tagagcacag ggctgtcatg ttggaagaat gttatatccg
     3781 tttccgtttc gaaggacaag gaacgattga tactatgtca gatgtccttc aacggcgaaa
     3841 cgtacgattt tatatcacaa tagattggag aaattaagat gaaaaaaacc ggttaaaaaa
     3901 acaggttata ccaagaatac acctaaaagc tatatcgtga atgctggtgc ggtgtataaa
     3961 aatttaacat ggaatactga gacccaaaaa tgggaaggtg agctatttgg agctacatca
     4021 ggcggaaata aactgacaat cgaacaaaat tatcgtcaaa ttgaagtaga tggtgtcttc
     4081 actgctgcgg ttggtcagaa agttcttgaa agtcaagggg ctaagttgga aatcaacgcc
     4141 aaagaattga ctgctgaaaa catccgtatg gctatcaacg gtgttattga gcaaggagat
     4201 ggtcagaaag ttccagctac atctaaggtc atcacagcta agggagagct taagaaaaca
     4261 gactacatta ctaatctagg cttagtaggt aacctttcag ggaccaatga acctatcatc
     4321 gtcattttag ataacgctct ctgtgtttct ggtcttgaat ttgaaacaaa ggataatgac
     4381 gaagcagtta tcacaatgac gttcgaagcg catgcaaacg aagaacagat tgatgatttg
     4441 acgttaccgt gtcgcatcat ctacccaact gtagatatgg aggtttaaga tggcagaaaa
     4501 acttgaaatg cgagagctaa atggtggaga catctttacg atgttaggga tcattgggaa
     4561 actagatatt aaagacgagg ttgtagaatt gctggagcgt caatttaacc aaccagccat
     4621 cgtcctgcaa gaccataaga agaagcaacc gacaaaagcc gagaaagaag cagcagaatt
     4681 agctacagaa aagcgcggta tgttgctgat gacggatatt ggctttgcta tcttgcgaca
     4741 cgtaggagaa gccaaaaatg atgttaacag ctttttagca gatttgacag gaactagtcg
     4801 aaaagatatt gagtctctta gcatgctggc ttatacaggt ttgctgatgg attttgctaa
     4861 aaaggcggaa ttgcgtgatt ttttccaatc tatttcttct gtcttggctt agatttgcat
     4921 cggctacgtg acattttata tcgacgttat ggaaatccag actctctctt gaagtcacaa
     4981 actttgaaag agtctttgga tttctttttg tatttgttcg atgaagagga taaagatgtt
     5041 ctcaaagaaa tttggttagc aaaagaagtg gacatgtctc ttcaaagttt tattgataaa
     5101 cactccaaaa aacgatatat cagcaaagtg aatcgtaagc aaattctaga aagcgataca
     5161 gattccgcat tgaaaaacgc agaatctatt ttgaatttgg gctatgggaa ggaggtgcga
     5221 ttagatggaa atctttaagt tatttggttc gatcggttta aaaaacaaag aagctaatca
     5281 agctattgat gagacgacgg gaaaagcacg gacagctgga gacaagatca aggatacatt
     5341 cggtaaagtt gctacattct taggaactgt ttttgcaggc aaggctatct ttgattttgg
     5401 gaaatcagtt gtagaggccg cagcgactgc aaaagcagta caagcacagt ttgaccaagt
     5461 ctttgaaggt atccgtgatg tagccgaaaa caagcttaat gatttagcta aaacggtcgg
     5521 tgccgtacct agtcgtatca aacctgcctt taaccaaatc gcttcgtttg ctaaagttgc
     5581 aggtatggat gccagccaat cgttagagtt tactacacga gctacagaag cagctgcaga
     5641 tagtgcagcc ttctacgata agtctttaga agaaacaact gagacgctca aatcttacct
     5701 caaagggaac ttcaacgtag cagataacct cggtattctt tcgacggaaa caacccgtaa
     5761 cgctaaggca acggaattat ttgggcaaaa gtataaagac ctcgatggta tacagcaaca
     5821 agaagtattg cttcggatgt ttgaggatgc taataaggtg tctggagcta tggggcaagc
     5881 ggcaagggaa tctgatggct ttgaaaacgt catgggaaat ctcaaacaga catgggaaga
     5941 tttcaaagtc cttgtcggag gaccgctgtt gcaaccagtg gtcgcaggtt tgcaacttgc
     6001 aacaaaatgg attcaagact taggcggtaa atttcaagag tttgagccta aaattacgtt
     6061 atttaaagaa ttgatagcat cggtatacga tgttattttt ggctctctga gcgtaaaaga
     6121 taatgtagat ttgctagtga agttggggat ggacgaggaa tcggtaaatg ccatcctatc
     6181 tactgctagt cttattggag ctgcgcttac tagtttttac gacatcattt ttggttctct
     6241 atctaaaaaa gacaacatag actttatgga gtcaatgggg atagacaaat ccactgctgt
     6301 cacaatagcg gatatagctg aaaatatccg agttagtttt gaaaatatag gacaggtagt
     6361 cagcgatgcc atttctatcg taggcgattt cattttcgag ctattaggag ttgctggtag
     6421 tgaaacaagc gtaaacattg ttgcagaagc gtttaaaact gtcacaggat tccttcgtga
     6481 cgcttcagaa aaactcaaag acttcacagg atttctaaaa agcaatcctg cagttcttga
     6541 cggcttgaaa tcggcggtgg ttggcctaac cgctgcttgg actagctaca aagtaatcac
     6601 gggagtcctt aaaggtattg agactgctcg aaatactctg ttagccgtag gaaacggatt
     6661 gatgttagct aggtttaccc aatcgggtgc gctaacggta gcagaagcaa cacaagcggc
     6721 cgccacaatg ggggctacag gagcattcag cgccttcaat gcagttttgt cgctcaaccc
     6781 tatcatgcta gtagttggtg ctatcgccgc actggttgca ggccttgttt ggttctttac
     6841 tcaaaccgag actggtcgtg agatgtggtc taatttcatt gagtgggcac aaaacgcttg
     6901 ggaaggaata ggagaatttt tctctaacct atggcaatcc atcacagaag gtgctatcaa
     6961 tctttggaat ggcatcgttg atacgtggaa tcatgctgtt gaaatagtca aaaacgtctg
     7021 gagtagcatt tctgaatttt tcacgaatct gtgggcagat gtgcagacga cagcaattga
     7081 ggcttggaat acctttgttc agggcgtgat gatggttgtc cagccgttca ttgatacctt
     7141 catgcaatat tggacaacga tgtctgatgg actttctcag atgtgggaag gggttaagat
     7201 gtttttccaa ggggcttggg aatttattaa agccattttc atgggagcag tcttaatcat
     7261 tcttgatatt gttacaggaa actttgggcg attgggtgct gacttagact tgatttggga
     7321 aagcattaaa tccgcaatca ctatggtctg ggacggtatc aagacctact ttagtggtgt
     7381 aatagacgct atcgtagcct tcggaactac ttattttgag aatttcaaaa atcttctctc
     7441 agctatttgg gaaggcgtga agtctgcggc gcaaggggca tgggattgga taaaaacaac
     7501 ggtatctaat cttataacca gcttgattga ttgggcacgt tctacatggg acggattcaa
     7561 gaattttctg tcaagtttgt gggaaggcat taagtctacc gcaacaaatg cttgggaaaa
     7621 ccttaagcaa ggcgtccaga atatcattga tggtctggtt tcgggagcca aaagagcttg
     7681 ggataatcta aaacagagcg tatctgattt ggtgtctagc gttacaagta tctttgacaa
     7741 cttgcgtaat atcgatcttt ttgcagcagg tcaagctatt ttggatggtt tcttaggagg
     7801 tttgaaatcg gcttggaaca atgttacagg atttatcagc ggtattgccg attggatttt
     7861 cgaaaacaaa ggacctattg aatacgatag aaaactcttg attcctgccg ggaatgccat
     7921 catggaaggt ctaggcggtg gactagaaga cggttttgac gatgttcaag atggagttct
     7981 tgggatggct ggaaaacttg ttcagaaggt ttctagtgca gctaagcatt ttaaagatac
     8041 agttgttcca cagatgggcg attggtcgtc ttctcttgat tggtacgcaa aaggcgaaat
     8101 catctcactt ccatccatgt atgatgtatc gtctagcaat cttttggaac attcgtcaga
     8161 cggtcagtct aatagcttgt tgcaaaagat tatcgaaata ttacaagcac tgcttgataa
     8221 agatagtgat gtctatctag atttagaaaa agttggtcga atgacctacg acgaacacgg
     8281 aaggattata gaaaggggag gataagctat gttagaacta aaagtagatg gtatcagcac
     8341 taagacaatt gtcgggtgta ttgtagctgg tttaggagaa gatagagctt cttctcctcg
     8401 ttttattgag gagactattt acggtatgaa tggaacaaat cggacaatcg aagcctacga
     8461 cgaggtggac agagtgttga cgtttcattg cgctagtctt gatgctgttc gccaactcat
     8521 aaaactattt aaaggcttag ataaaaagtt ggaattctgg catatcccaa atagtttcta
     8581 ctatttcgat tacaagagta gcagttataa aattaatcac aagtgtagtt gggacgtctc
     8641 cgttaaaatt agcttgatgc cttttcgtta tctaagtgag gtagaagata ttgtcttaac
     8701 tcaatcggga gctatcacaa acatgggtga tatttttagc gagcctagga ttgaggtgtt
     8761 cggaaatggt caaactagct taactattgg aaatcaagtg ttaagactga atttagatac
     8821 taaagctgtt atagagtgcc ggcatgggta tcaaaatgta tatgataaaa atggaattat
     8881 caagaatagc attcgcacat caggtccgtt cttcgaaatt cctgcacagg ttacaagtgg
     8941 tgttgttttg ggttccggaa tcaatagggt tgtaattcgt cctagatgga ggtgcgtgat
     9001 ttgattagtt taaaagataa aaatgttttg cttgtcaatg cttttgatga tgaaatcgaa
     9061 caagagggta acggcaagta tcaactttct tttaaatacc cgacatctga tcagcgttgg
     9121 aaaaacatag atttagcaga tttactgtta gcagacgatt tgcatggcga gcaggagttt
     9181 cgagtttttg agattgtaaa acgaaatggt tatatttttg tctacgctaa tcaggttgta
     9241 gatgatttaa acagctactc gatcagtaac atctctgtag atagagttaa tggtcaaact
     9301 gttatgcaag ctttggcagg cagtattatc cgaaaacatc cgttcgcttt ttatagtgac
     9361 attcaggata ggcacgtttt taaccaaaaa aacatcagcg ttatgggcgc tctggcaaat
     9421 ggtaaacatt cgattattgg acagtggggt ggagatttag ttagagataa atataacgtt
     9481 aatttgttaa aacgaggagg gcgagaatca gaagctcttt ttatgtataa gaaaaactta
     9541 aaatcttatg cagaaacaaa ttctataaaa gggctattta ctcgtttaaa cctctctgtt
     9601 aagaattcgg acataaaaac aaccgtagac agtcctttga taggctctta tggcgggagg
     9661 gtttatgagt taaacgtcga ggttgcagat caagatgtgg caactttaga gcagctgcaa
     9721 gagtatggtc ggaactattt tgcagaaaaa ttaatcgatg taccaaacaa tagtttgacg
     9781 atttcggtca cagacagtca ggaatatgat gtccgaatat ttgacacggt ctttgttcat
     9841 cacgaaattt ttgacaaaga ccttcggatg aagattacta gctacagatt tagtccgatg
     9901 ggtcgtaaat taaaatctat tggttttggg aaaattaaaa ctagcctcac tcaacaagtg
     9961 tcaaatatcg ctactgaagt tgcagaacag tcggtatctg tagcagggca agttttcgaa
    10021 gaaaaactac aaaaagagat cgaaaatgcc gataaagtta tcgatggcaa gattaataag
    10081 gttagagatg aaatcaacga cggtctcgag cagtcaaaag cggaagcaga agcgtatgca
    10141 gataatatca agcaacggat agatggacag ctcgccgaat cggaccgtca atatcaactt
    10201 gcccagcaaa cccaaggccg tcaaatcgca gatgtactag ctaaggctac tgcggctaaa
    10261 gaaatagctg aacaaactgt aacagattta atgcgggtac aaaacgcatt caaccaatcc
    10321 attggtcaag ctaataccaa agcgctcgaa ttagagcggt ctattggtac agttcggaca
    10381 gatgttatgt ctcaagcgca aactatactt gctcaggcac aaacacagac ggaattgacc
    10441 aatcgtgtga caaccgttga aactctggca gatggcacga agacaacagt cgcagagctg
    10501 tctaaaacag tttctaaggc gactggagac atcaccagtg tcacaagtcg gaccaagacc
    10561 gtagaagaga ctctgagcca aacgaggact caatatgagg ccttgacgca gaccgtcaat
    10621 actcagacag ggcagattga gagtatcaat cgcaagactg ccgacttgca gagcgggatc
    10681 gacggcgtga cggagcggtt tgagagtttg agggtaggtg ctaccaatta catcagagat
    10741 tttggttttg ccaaaaatca caattttagt agctaccagt cgatttggca atttgaacgc
    10801 atctcagact ctactgcacg tagcggatat cttgtaaaag caacctgtac acaagcaggc
    10861 ggggctggtt tctaccggag gttttttgat ttgagaggtg aggaatggca ggggcgcaca
    10921 atgacctatg ctgttgatat aaaagcatct aaatcagtcc ggatgcgact aggtgccgaa
    10981 gcatttaata agagctatag agcttttgat gtaacgacag attggcagag attcgtttcg
    11041 acagatacgg tcaattataa aacagactat ggcttcacgt tttatataga cggtgcccaa
    11101 tggcaagttg gagatattgt gtatatccgc gaccctcaat tagaggacgg aagcatcgcg
    11161 actacaccac atgcatctga ggaagatttg aggtctgaat tcgctacata cgtccgaaat
    11221 gcagctgaaa acagtgcaga attggttaga cgaattgaaa ctgcagatgg aaaagcagta
    11281 gatgttaaga cctacgccca gcagacagcg actgcaatca atactcgatt agagagccta
    11341 gaaacctaca agaacgcgga aggcacacga gctagtcagt actttagcgc tagtcgagac
    11401 gagacagcaa ggaaagtaac tgctctgcgt acggcagtca cagacggcta cgtagctaaa
    11461 gctaagtacg aggaagatgc ccgaggtgtg atgcagaggt ttgagagttt atctgtagac
    11521 cagcaatcct acacagatat caaaatagcc gaatacaaga ccacagttga cggacgattt
    11581 gcgacgatgc agacatctat tgatagtaag gctagtcaag ttgcgtttca gcgggtgcag
    11641 gagacctcac agctctatga gcgtatcatc ggctcaacgg aaactggtat caaagacaaa
    11701 gtcgctcgta tggtcatggc tgatagtcta tttctaaccg aggtcaagga taagattagc
    11761 ggtacagcta cacaggttag tcagcttaat aattcgtacg ctattaaaaa tctgactagt
    11821 gctggtacag tgcttaacca aatcaattta ctggctaatg gtactaatag aatcgatggt
    11881 cgactgacgc atatcacagg tcagactttg attgataatg gcgtaatcaa gaacgctatg
    11941 attggcaatt tggacgcagg aaagattaat acaggtacgc tcaatgcagc tagaattgcg
    12001 actaacagta ttgatggtag taagcttgtg tttgaccaag cgtttgtcaa taagatgact
    12061 gcaaacgaag ccttgtttaa gcagttattc gcaaagaatg cattcattac aagcgtgcag
    12121 gcagttacgc tatcggctag tcaaattaca ggtggattga tgagagccac gaacagggcc
    12181 atggaagtca atcttaatgc aggtcaaatc ttgtactata cggaccaagc ggcactcaaa
    12241 cgtgttttaa acggctatcc cacccagttt gtcaaattcg caacaggcac ggttaccggc
    12301 aagggaaacg caggtgtgac cgtgattggc tccaaccgtt ggaactctga atcgtcaaat
    12361 gatggcggat ttgtcggaat tagggcttgg aacggtccaa atattgacca aatcgacgtg
    12421 gttggagata cggtcagatt ggcaagctca gcgtttgaag cagcggatgg atggaatatc
    12481 aataccttgc ctgggaaatt agacatcgat gcatttaatg ctgatcaccg cgcttcgtcc
    12541 aagataaaag ttggggattt gtggttgtgg aaaaacgcta cgacatactc gagcatgaga
    12601 gataccataa atttgatcat tgacaactta cagctattac acaacaataa atcgacagag
    12661 aggggttata gctatactct accagcaaaa gtttaggagg aaacatttaa atgaaccaag
    12721 aacaactgaa tcaagcactc gctttgacaa accaagaact agccaaccaa ttggcagaag
    12781 agaagaacac taagaatctg ttagctgtac agctgaccga agcacagcag actatcgcta
    12841 gtctgcagac agaaattaag gaacttacac agcaactgga cgaagctact aaaccagcag
    12901 aaatcataga agaaggagaa tgatcatgcc acttgaaact ataaaaacta ctcaattaat
    12961 cggaaactta aaaatcggtg atgagattgt caaaacttat acagtcaata tcgataataa
    13021 aggcgtatcg aggattttcg aaactgtata caatcaagaa ctctatgcgg ctaaccgcaa
    13081 agagatgcgc aagcaagaag ctgagttccg tgaaaaacgc tacgaggtgg aagatgctat
    13141 cttggctgaa cttgagccga aggaagagta gcctatgcta ggacaagata tcgttagaga
    13201 ggcaatcaaa gcctcttgga caatagataa aatcgcgggg gtggttgtcg tcatcctcgc
    13261 gataatgctt gtctcgatgg ttgtcagtca aaatgccaat gtcaagcgat tgattaataa
    13321 tttccaaaaa accaatgatg ctttgatgga gactaaccgt cgaatcgcta gcgacaatca
    13381 gcgtcacatg gaacatttga caacagcggt caataatttg gcaagtgaga cacgtaatga
    13441 tattgctgat ttaaaagaac aggtctcaga gctcaaagag gaggtaagag accaacgaat
    13501 gatgccgtag gaggtagaat atggaacatt taacagagct tattttaggc acagcgacag
    13561 ggatttttgg tattgttgga ggcatgattg tccacgaggt caaaaagtat ctaattgcca
    13621 agggcggtaa gcgagcggtc gaaattacag agattctggc acggaacgcc gtcaacgcag
    13681 ttgagcagat taccaaatta gaccaagata agcacgtcga taagctagat atggccaaac
    13741 gtcgtgtaac aggtcagctt gctaaataca acatctacat gactgatacc cagttagaga
    13801 cctttatcga atcagcagtg aagcaaatga acgatgcctg gaaggagtaa ctatggtaat
    13861 caatcttgaa acatccattc gttggatgag cgaccgtgtc ggcaaagtct cttactcaac
    13921 ggactatcgt aacggtccga atagttatga ctgctctagt gctgtatatt atgcgctaat
    13981 ggcaggcggt gcaatttctg caggttgggc ggttaacac
//
LOCUS       DATUCG010000060        13703 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.061, whole genome shotgun sequence.
ACCESSION   DATUCG010000060 DATUCG010000000
VERSION     DATUCG010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13703
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.061"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            43..1374
                     /locus_tag="VCA20_001391"
     CDS             43..1374
                     /locus_tag="VCA20_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excalibur calcium-binding domain-containing
                     protein"
                     /protein_id="HEP1835203.1"
                     /translation="MSNKENDLCLRKKATQIVSETSIKKSRKFLNRKTAGITGGVLLT
                     TFIGSQLMTAELPKKDDLYGQQYVTVVKHLQEAGFKNIQGVELSDLEFGKIGESDLVE
                     LVSVDGEDWKEGRALKNIPITISYHLPKKDAVEFNLPASKNLADVEKELKDSGFKQIE
                     LTPVLLVEEGNADKKDKIDRLQIGNHTYQSNHFYSTSLPVTLTYFDVSKDNIKLPENL
                     AEAKTKPELEKQLKTAGFTDIKWTAVADKDKAKHEKIQKISLGGAELQLPTKQEIISK
                     KSTPIVITYYDFSSFAELPSSISTKTAADTKKLFTDGGFSQVSEVATETNEIAKNGQI
                     IAVEIDGKSFNEMNDKVIKKDSKVIIKYWNAEKAIAEKARKEEEERLAAEAQKVAEAQ
                     SQVQQFAATPSQNTYYPNCKAVRQAGAAPIYRGEPGYGSHLDRDGDGVGCE"
     gene            1759..2340
                     /locus_tag="VCA20_001392"
     CDS             1759..2340
                     /locus_tag="VCA20_001392"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEP1835204.1"
                     /translation="MAQLYYKYGTMNSGKTIEILKVAHNYEEQGKPVVIMTSALDTRD
                     AFGVVSSRIGMRRDAVAIDDDMDIFGFIEKMEPRPYCVLIDEAQFLRRHHVYALARVV
                     DELDVPVMAFGLKNDFRNELFEGSQHLLLLADKLDEIKTICQYCSKKATMVLRTQDGK
                     PTYEGEQIQIGGNETYIPVCRKHYFSPEIKELP"
     gene            2851..3930
                     /gene="prfA"
                     /locus_tag="VCA20_001393"
     CDS             2851..3930
                     /gene="prfA"
                     /locus_tag="VCA20_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEP1835205.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSKEEAATR
                     DTVTAYLEYKTVLQNIVDAEEMIKESSGDADLEEIAKEELKQAKADKEAYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAEGQGWRFEVMEASYNGVGG
                     IKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLIMPEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRIVHLPTNIKVEMQEERTQQKNREKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTIGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKMDEVVDALVLYDQTQKLEELNK"
     gene            3930..4763
                     /gene="prmC"
                     /locus_tag="VCA20_001394"
     CDS             3930..4763
                     /gene="prmC"
                     /locus_tag="VCA20_001394"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002934985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEP1835206.1"
                     /translation="MNYAQLFAAYEEQLVAIGEEAESLSFTFRGLKGLNFTEFLLLLR
                     QEVTPTDKEEIDAIFQQLSQHRPAQYIIGKADFHGLEFAVDERVLIPRPETEELVDLI
                     LQENSGAGLRILDIGTGSGAIAISLAKARSDWEVVAVDISKDALAVAQENARTNQVSV
                     HFLESDVLQAVTGYFDIIVSNPPYISPDDTDEVGLNVLTSEPHLALFAEEDGMAIYRQ
                     IAEQAGAFLKENGKLYFEIGYKQGQDLPDLLALHFPKKRVRVLKDQFGQDRKVVADDN
                     G"
     gene            4750..5358
                     /locus_tag="VCA20_001395"
     CDS             4750..5358
                     /locus_tag="VCA20_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEP1835207.1"
                     /translation="MTMDKLRTILENGGAVILPTETVYGLFAQALNEDAVNRVYQLKQ
                     RPRDKAMNLNVSNLNDIYFFSQNTPFFLEKLYNRFMPGPLTIILKANDNVPFWVNSGL
                     DTVGFRLPNHEQTLRLISETGPLIGPSANISGNESGKKFSDIQAQFSVDLPGIEDDQA
                     LTGIDSTILDLSGQKARILRQGAISREEIQKAIPEIEFEVVL"
     gene            5355..5786
                     /locus_tag="VCA20_001396"
     CDS             5355..5786
                     /locus_tag="VCA20_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEP1835208.1"
                     /translation="MIRPIEKSDLVVIREINAQSLGYDCSLEQTERQFFRCTSTLGHI
                     LLVYIDDISGAVQGYIHAQVYESLYSDTGLNILGLAVLPGHQGQGIGASLLKAVEQIA
                     QKEGYHFIRLNSAESRLQAHLFYEKNGYHSDKMQKRFIKHI"
     gene            5810..7069
                     /locus_tag="VCA20_001397"
     CDS             5810..7069
                     /locus_tag="VCA20_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEP1835209.1"
                     /translation="MIFDKVNYKELDKEVWEAIQAEEKRQQNNIELIASENVVSKAVM
                     AAQGSILTNKYAEGYPGRRYYGGTECVDVVESLAIERAKEIFGAKFANVQPHSGSQAN
                     CAAYMALIEPGDTVMGMDLAAGGHLTHGASVSFSGQTYNFVAYNVDEETGLLDYDAIL
                     KQAKEVQPKLIVAGASAYARTIDFAKFREIADAVGAKLMVDMAHIAGLVAAGLHPNPV
                     PHAHITTTTTHKTLRGPRGGLILTNDEELIKKINSAIFPGIQGGPLEHVIAAKAVSFK
                     EVLDPDFKDYAQKVIENSKAMAEVFLANPNFKVITGGTDNHLFLVDVTKVVENGKVAQ
                     HLLDEVNITLNKNSIPYEKLSPFKTSGIRIGSAAITARGFGVEEARKVAQLTIKALEN
                     AENEKALEEVRQEVRALTDQFPLYEGL"
     gene            7069..8043
                     /locus_tag="VCA20_001398"
     CDS             7069..8043
                     /locus_tag="VCA20_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEP1835210.1"
                     /translation="MDIFVKKAIIHQFSPDDTELVLSDQLLTVSPKIEEYLRKKIERV
                     FSDEAKTGQFNPDNPFLDYLDGDLLTNSVKIANLWKEEFSISENLKTNDLIFIEFERN
                     GVEHFAFLRISLRENLAHIGLESGSPLKITQNNLPGAGSAPDEALIVNLQTRKYHLIE
                     KRIKHNGAFLNYFSDNLLQVTPAISAKKSIKAVEQTAQKIADNFHQGDFQFQSKVKSA
                     IFNNLEEDNELSPEKLADQLFDNNLTARLNFVDQLKEVIPDKISFDEIDSSRQLKKFE
                     NQKLSLSNGIELIVPNAIYEDAESVEFIQNDNGTYSILIKNIEDIKSK"
     gene            8044..8643
                     /locus_tag="VCA20_001399"
     CDS             8044..8643
                     /locus_tag="VCA20_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HEP1835211.1"
                     /translation="MKKLIRTLILLLLIFLGYKFYQTYSAVKQVMTYQTMVQEVLAEN
                     DTPANEELVLAMIYTETKGQEADVMQSSESATGYANTITDSRESIRQGIIYLTENLRL
                     AEEKGVEVWTAVQAYNFGPAYIDYITEHGGEHTLSLAKEYSRTVVAPSLGNTTEETYT
                     YFHPLALLNGGKLYVNGGNIYYARQVRFNMHLMRLINLF"
     gene            8744..9502
                     /locus_tag="VCA20_001400"
     CDS             8744..9502
                     /locus_tag="VCA20_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002934991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEP1835212.1"
                     /translation="MKRNVLITGASSGIGKAIAYVFAANGDNIIITGRRLDKLEEIKQ
                     DIEKQYHVQVSVHSFDITNMEQTIVNCQKILEEVGQIHILVNNAGLALGLDKFQDYDL
                     TDMMTMIDTNIKGLLTVTRQLLPQMVENNEGHIINIGSTAGIYAYAGAAVYAATKSAV
                     KVLSDGIRIDTIDKNIKVTTLQPGIVETDFSQVRFHGDKERAATVYQGIDALQANDIA
                     SCTLFVANQPAHVQISDMTIMATKQATGFTIHRD"
     gene            9519..10268
                     /locus_tag="VCA20_001401"
     CDS             9519..10268
                     /locus_tag="VCA20_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor 1 (IF-1)"
                     /protein_id="HEP1835213.1"
                     /translation="MKRKYLALALGLLTIISLSACQTEKKGSSASTSTADTQVSNANT
                     STSSTIQREISLSSMDIDALMQGNYDSILGTWQNSQGNSLVFNSKGLVADSQSLFGRG
                     KITDGIFETGYVDATIGDVTLLMIPKGTQANTTEIDTTDSTRDRMIVISQETTADVVD
                     VYYRLTNSATSGIDPLKNTETGVQLDSGPKTIDYANSILGENNWRVIEGNYTRTEFIP
                     YNILEGDDNARYTIYQNGVIINADYQIVYQP"
     gene            10290..10643
                     /locus_tag="VCA20_001402"
     CDS             10290..10643
                     /locus_tag="VCA20_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HEP1835214.1"
                     /translation="MTFTSFEPTRNVQDFHLAAFAYYDGLDVIDQLKPGTPVQLVGEP
                     SNPHDSEAVAIFYQDTKLGYIPSDKNSLISRLIYFGHGDILEARIQMSNTENHPDRHF
                     RVVVKLKDNRKNDSR"
     gene            complement(10664..11611)
                     /locus_tag="VCA20_001403"
     CDS             complement(10664..11611)
                     /locus_tag="VCA20_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835215.1"
                     /translation="MDVFPSFFIFPFNKLNVFVDLKNIFCYTCVMKTSTLGKLLELPN
                     FHHFYTVPIRENTESSGNYIIVFEQDKQPVFVDLKNETGHTREVRFASHFDSAGLLVG
                     LGAIPINCWPTCQILGVKKNNNRRGLSLSSLMDGYGRIPIFKEMAERYRARGQSTIYD
                     PYRYSYTIDEKKVIKDNQLTLIPKKEYEKIKDFHPFLQTKHGNVYAVPCFYQDRKRNQ
                     ITKIYIMGWYFYEKQLKISWKPSHSRKSRWRSSLVSVDKLSDRFFFQGELYKKGTYPV
                     FLEDLTIKNKAYQHRLDREFAAFLSIIEEQKQRAQLRVK"
     gene            complement(11639..12427)
                     /locus_tag="VCA20_001404"
     CDS             complement(11639..12427)
                     /locus_tag="VCA20_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835216.1"
                     /translation="MAVLKYSKVLLLVLLIATGLSCIGIYWLGKEQNRLLNEQCHALN
                     IRIINDLGTKIDAIGGPQNPRIIGFFQQDDTTAISQRIGTASEEELKIANPDNLFQKE
                     WIVLYPQTRSSPFENTSAYAVMKTSIKADWLHVTTSSETELDIFYEKADESLLTLEDL
                     VQDKESFRTTLKTILVSAKNEDEIQVQKDILEMFESDDWSAIPFAYTEKSLILEKAVI
                     SISAFVDSLNPYYFSEQTLADLRLSEESRQALEDSVDKTIITYP"
     gene            complement(12484..13347)
                     /locus_tag="VCA20_001405"
                     /pseudo
     CDS             complement(12484..13347)
                     /locus_tag="VCA20_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987985.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
BASE COUNT         4438 a         2659 c         2758 g         3848 t
ORIGIN      
        1 atattgcaaa atatatttat attttgttat aataatatag caataagcaa taaggagaat
       61 gatttatgtt taagaaaaaa agcaactcag atagttagcg aaacgtcaat taaaaaatct
      121 agaaagtttc ttaatcggaa aacagcagga ataactggcg gcgtcctact taccacattt
      181 atcggttcac aattaatgac tgctgagctt ccgaaaaaag atgaccttta tggacaacag
      241 tatgtaacag tcgtaaaaca tcttcaggaa gcaggtttta aaaatattca gggggtggaa
      301 cttagtgatt tagagtttgg aaaaataggt gaaagcgacc ttgtcgaact cgtctctgtt
      361 gacggagaag attggaagga aggacgtgct ttaaaaaata tcccaattac aatctcctac
      421 catcttccca aaaaagatgc cgttgaattc aacctaccag cttctaaaaa tttagcagat
      481 gtcgagaaag agttgaaaga ttcaggtttt aagcaaatcg aactaactcc tgttctgcta
      541 gtagaagaag gcaatgcgga caaaaaagat aaaattgatc gccttcaaat cggaaatcac
      601 acctatcaat ccaatcattt ttactctact agtctccctg ttacattaac ctattttgac
      661 gttagcaaag ataacatcaa actaccagaa aatctagcag aggctaaaac aaaaccagag
      721 ctcgaaaaac aactaaaaac agcaggattt acagacatca agtggactgc tgttgcagac
      781 aaggataaag caaagcacga aaaaattcaa aaaattagtc taggtggtgc agagcttcaa
      841 ttgcccacca aacaggagat tatcagcaaa aaatcaaccc ccattgtaat tacctactac
      901 gatttctcta gcttcgctga actgccatca tccatttcaa cgaaaacagc tgcagatact
      961 aaaaaattgt ttactgacgg tggtttttct caagtatctg aagtagcaac agagaccaat
     1021 gaaattgcta aaaacggaca aatcattgcg gtcgagattg atggcaaatc ttttaacgag
     1081 atgaacgata aggtcatcaa aaaagatagc aaagtcatta tcaaatattg gaatgctgaa
     1141 aaagctattg ctgagaaagc acgtaaagag gaggaagaac gtctcgccgc cgaagcgcaa
     1201 aaagttgcag aagcacaaag ccaagttcag caatttgctg ctacaccatc tcaaaacaca
     1261 tattatccaa attgtaaggc agttagacaa gcaggagctg caccaatcta tcgtggagaa
     1321 ccaggttacg gtagtcattt agaccgtgat ggagatggag ttgggtgtga gtaagaaaaa
     1381 acattgacaa cgtttacact gcgtgctata ctactcttat aaggagggat tactcatgac
     1441 ctataaatac cgtatgattt tatcgttcct actcactgga ttatttttgt atctagtggc
     1501 aactgttttt gcccaaagta tatggcaagg cccattattg atctcattct ccttcttcag
     1561 cttaatctat agttgtgtta tgctctacag gtggaaacca atggcagcta aaatcatttt
     1621 ccgatgtatt ggagaatttc tctctttacc atggtcttaa tatcaaacaa gctcaaaacg
     1681 ggcttgtttt tctatttcct aaatggtcaa tttatgatag aatggaggga gtgaaaattt
     1741 aagattggaa agagaaaagt ggctcaatta tattataaat atgggacaat gaactcaggg
     1801 aaaaccattg agattttgaa ggtggcccac aactatgaag aacaaggaaa acctgttgtt
     1861 atcatgacat ctgcacttga tactcgtgat gcctttggtg ttgtatccag tcgtataggc
     1921 atgagaagag atgcagtggc gattgacgat gatatggata tctttggttt tattgaaaaa
     1981 atggaaccgc gtccttactg tgttctcatt gatgaagcac aatttcttcg acgtcatcat
     2041 gtctacgcac ttgctcgcgt agtcgatgaa ttagatgtac ctgttatggc tttcggtctt
     2101 aaaaatgatt tccgaaatga actgtttgaa ggttcccaac atttgctctt attggctgat
     2161 aaattagatg aaatcaaaac aatctgccaa tattgttcta aaaaagcgac aatggttttg
     2221 agaacacagg atggcaaacc tacttatgaa ggagaacaaa tccaaattgg tggcaatgaa
     2281 acctacattc ctgtctgtcg caaacattat ttttcaccag aaattaaaga attaccctaa
     2341 tttttgaaaa tgaaatgaga agcgactgta aactgagcaa ctatatagaa ctgaatttgc
     2401 ctatgactct gtgccaattt tcataactta catactacgg caaaggaatt gaatacgcct
     2461 acgactctgt ggaaatagat aattcttcct agaaaccgaa gtttccacgt aagaattcct
     2521 aatttccttt gagtcgctta acggctttaa tatcttaatg gaattgaaca cgccctataa
     2581 gctgtgtcaa aaagataatt tctccaagtg tttgtaacac ttggtcaaaa ttcctatttt
     2641 gactatgctt gcttaacggg ctttgtatct taatggaatt gaacacgccc tacaagctaa
     2701 ctagaagtgt gttcggtcaa atatttagaa aggaattgaa tacgggctac gaactgtgca
     2761 aaaaagatag tttttcctag gacgctttcg tccttcgtca aaactcctat ttttgctttg
     2821 ttcgtttaac gcccttaata tcttaaaatt atgaacatct acgatcaatt acaggcggtg
     2881 gaggaccgct atgaggaact tggtgaattg ttgtctgacc ctgatgtagt cagcgatacc
     2941 aagcgtttta tggagttgtc taaggaagaa gctgcaactc gtgatacagt aacagcctac
     3001 ctcgaatata agactgtact tcaaaacatt gtcgatgcag aagagatgat taaagaatct
     3061 tctggtgatg ctgacttgga ggaaatagct aaggaagaac tcaaacaagc taaggctgat
     3121 aaagaagctt acgaagaaaa gctgaaaatc cttcttttac caaaagaccc taacgatgac
     3181 aaaaacatca tcttggaaat ccgtggtgcc gcaggaggtg atgaagctgc cctctttgct
     3241 ggtgaccttt tgaccatgta tcaaaaatat gcagaaggtc aaggctggcg ctttgaagtc
     3301 atggaggctt cttataatgg tgtcggtggt atcaaggaag tggttgccat ggtctctggt
     3361 caatccgttt attcaaaact caagtatgaa tcaggagctc accgtgtcca acgtgtccca
     3421 gtgacagaaa gccaaggtcg tgtccacacc tcgacagcaa ccgttctgat catgccagaa
     3481 gttgaagaag tggaatatga catcgatcca aaagatcttc gtgtcgatat ttaccacgct
     3541 tctggtgctg gtggacagaa cgtcaacaag gttgcgactg ccgtgcgtat cgttcacttg
     3601 ccaacaaaca tcaaggtgga gatgcaggaa gaacggacac agcagaaaaa ccgtgaaaaa
     3661 gctatgaaga ttatccgtgc ccgtgttgcg gaccactttg cacagattgc ccaagatgag
     3721 caggatgcag agcgtaaatc aactatcggt acaggtgacc gctcagagcg tatccgtacc
     3781 tacaactttc cacaaaaccg tgtgacagac caccgtatcg gcttgacctt gcaaaagcta
     3841 gatactatct tgtcaggtaa gatggacgaa gtcgtagatg cccttgttct ctacgatcaa
     3901 acgcaaaaac tagaagagct taacaaataa tgaattacgc tcaattattt gcagcatacg
     3961 aagaacaatt agtcgcaatc ggtgaagagg cagaatccct gtccttcacc tttcgtggct
     4021 taaagggact gaactttacg gaatttctcc tcctgctccg tcaggaagtc acaccgactg
     4081 acaaagaaga gattgatgcg atttttcagc aactttccca gcaccgacct gcccagtaca
     4141 ttatcggcaa ggctgatttt catggcttgg agtttgcggt ggatgagcga gttttgattc
     4201 caagacctga aacggaagaa ttagttgatt tgattttgca ggaaaatagc ggagcaggtt
     4261 tgcgtatctt agacatcggt acaggaagcg gtgccatagc tatttccctt gcaaaagcta
     4321 gatctgattg ggaagtggta gcggttgaca tttcaaagga tgccctagca gtggctcagg
     4381 aaaatgcaag aaccaaccaa gtctctgttc actttctaga atcggatgtc ttgcaagcag
     4441 tgacagggta ttttgacatc attgtttcta atccgcccta tatctcacca gatgacacag
     4501 atgaagttgg acttaatgta ttgacttctg agcctcatct ggccttattt gcggaagaag
     4561 atggcatggc tatctatcga caaattgcgg agcaagcagg ggcatttttg aaggaaaatg
     4621 gaaaactcta ctttgaaatt ggctacaagc aagggcaaga cctacccgac ttactcgctc
     4681 tgcatttccc caaaaagcga gttcgagtcc tcaaagacca atttggacag gatagaaagg
     4741 tcgtagcaga tgacaatgga taaactccga acaattcttg aaaatggtgg tgctgttatc
     4801 cttccaactg aaacagtcta tgggcttttt gcccaagcct tgaatgaaga tgcagtcaat
     4861 cgtgtctatc agttgaaaca gcgtccgagg gacaaggcaa tgaacttaaa tgtctcaaat
     4921 ctgaacgata tttatttttt cagtcaaaat acaccctttt ttctggagaa actgtataat
     4981 agatttatgc caggaccact aacgattatc ctaaaagcaa acgataatgt tcctttttgg
     5041 gttaattctg gactggatac tgttggtttt cgtcttccca atcacgaaca aaccttacgt
     5101 ttgatttcag agactggccc actcattgga ccgtccgcca atatttcagg aaacgaaagt
     5161 ggcaagaagt tttcggacat ccaggcacaa ttttcagttg atttgcctgg cattgaggat
     5221 gaccaagctc tgacaggcat tgattcaacg attttggacc tttctggaca gaaagcgcgg
     5281 attttgcgcc aaggtgctat cagccgcgaa gagattcaga aagccattcc cgaaatagaa
     5341 tttgaggtag ttttatgatt cgccccattg agaaaagtga cttggtagtc atccgagaaa
     5401 tcaatgctca atccttaggg tatgattgtt cattggaaca aactgagcga caatttttta
     5461 gatgtacgag tacattgggg catatcttgt tggtctatat agatgacata agtggcgcag
     5521 tgcaaggcta cattcacgca caagtctatg aaagtctcta ttcggataca ggcctcaata
     5581 ttctcggact agctgttttg cctggtcatc aaggacaagg gattggagca agccttctga
     5641 aagccgttga gcaaattgca cagaaagagg gctaccattt tattcgcctc aattctgctg
     5701 aaagtcgtct ccaagcccat cttttctacg aaaagaatgg ctatcactcc gataaaatgc
     5761 agaagcgttt tatcaagcac atttagccca atacaaagga gtaccgtata tgatttttga
     5821 caaagttaac tacaaagaac ttgataaaga agtttgggaa gctatccaag ctgaagaaaa
     5881 acgccaacaa aataatatcg aattgattgc gtctgaaaac gttgtttcta aagctgttat
     5941 ggcagctcaa ggttctattt tgaccaataa atacgcagaa ggttacccag gtcgtcgtta
     6001 ctatggcggt acggaatgcg ttgacgtagt tgaaagttta gctattgaac gtgctaaaga
     6061 aatattcggt gcaaaatttg ctaacgttca gccacactca ggaagccagg ccaactgtgc
     6121 tgcctacatg gccttgattg agcctggaga cactgttatg gggatggact tagctgcagg
     6181 tggtcacttg actcacggtg catcagttag cttctcagga caaacttaca atttcgttgc
     6241 ttataacgta gacgaagaga caggtttgct ggattacgat gcaattttaa aacaagcaaa
     6301 agaagtacag ccaaaattga ttgtagcagg tgcttcagcc tatgcacgta caattgactt
     6361 tgctaaattc cgcgaaattg cagatgcagt gggtgccaaa ctgatggtgg atatggctca
     6421 tattgctggt cttgtagcag caggtcttca tccgaaccca gtgccacatg cccatattac
     6481 aacaactacg actcataaaa ctcttcgtgg gccacgtggt ggtttaattt tgaccaatga
     6541 tgaagagctt atcaagaaaa tcaactcagc catcttccca ggcatccaag gtggtccatt
     6601 agagcatgtt attgctgcaa aagccgtatc tttcaaagaa gttttggacc cagatttcaa
     6661 agactatgct caaaaagtga ttgaaaacag caaggccatg gctgaggtct tccttgcaaa
     6721 ccctaacttt aaagtcatca caggaggaac agacaaccat ctcttcctcg ttgacgtgac
     6781 caaagttgtt gaaaatggta aagttgctca gcatctcttg gatgaagtaa atattactct
     6841 caataaaaac tcaattcctt acgaaaaact ctcaccattc aaaacaagcg gtatccgtat
     6901 tggctctgcg gccatcactg ctcgcggttt tggagtagag gaagctcgca aggttgcaca
     6961 actaactatc aaagcacttg aaaatgcaga aaatgaaaaa gctcttgagg aagtccgtca
     7021 agaagtgcgt gctttgacag atcaattccc actttacgaa ggtttataat ggatatattt
     7081 gtaaaaaaag ccatcataca tcaatttagt cctgatgata cagagctggt cctttccgac
     7141 cagcttttga ctgttagccc aaaaatagaa gaatatttgc gtaaaaagat tgaacgtgtc
     7201 ttttctgatg aagcaaaaac aggacaattt aaccccgaca atccttttct tgactattta
     7261 gatggtgacc tactgaccaa ctctgtcaag attgctaatc tatggaaaga agaattttca
     7321 atttctgaga atttaaaaac caatgacctg attttcatag agtttgaacg aaatggtgtt
     7381 gaacattttg cctttttgag aatctcactg cgtgaaaatc tcgcccacat tggtctggaa
     7441 agcggaagtc ctttaaaaat cacccagaat aatttacctg gagcaggttc agcacctgat
     7501 gaagccttga ttgtcaatct tcaaacgcgg aagtatcatt tgattgagaa aagaatcaaa
     7561 cacaatggag ccttcctcaa ttatttctca gataatcttc tgcaagttac accagctatt
     7621 tctgcaaaaa aatcaatcaa agcagtagag caaactgcac agaagattgc tgacaatttc
     7681 catcaaggcg attttcaatt tcaatcgaaa gtcaagtcag ctattttcaa caatttggaa
     7741 gaagataatg agctttcacc cgaaaaattg gctgatcagt tattcgataa caacctgacc
     7801 gctcgtttaa atttcgtcga tcaactgaaa gaggtcatac ctgataagat ttccttcgat
     7861 gaaattgata gtagccgtca acttaagaaa tttgaaaatc agaagctatc tttatcaaac
     7921 ggaattgagt tgattgtgcc aaatgcaatc tatgaggatg cagaatcggt tgagtttatt
     7981 caaaatgaca acggaaccta ttctattctt atcaaaaata ttgaagacat aaagagcaag
     8041 taaatgaaaa aactgatacg tacgctgata cttcttttac tgatttttct cggttacaaa
     8101 ttctatcaaa cctacagtgc agtcaagcaa gtgatgacct atcaaactat ggtacaagaa
     8161 gtattggcag aaaacgacac accagctaat gaagaattag tcctagcaat gatttacaca
     8221 gaaaccaagg gacaagaagc agatgtcatg caatcaagtg agtccgctac tggctatgct
     8281 aacactatca cggatagtag agagagtatc aggcaaggaa ttatctacct aacagaaaac
     8341 ctacggttgg ctgaagaaaa aggagtagag gtttggacag cagttcaggc atataatttc
     8401 ggtccagcct acatcgacta tattaccgag catggtggag aacatacact ctctttagct
     8461 aaagaatact ccagaacagt agtagcacca agcttaggga atactacaga agaaacatat
     8521 acctattttc atcccttggc tttgctaaat ggcgggaagt tatatgtaaa tggtggaaat
     8581 atctactatg ctagacaggt tcgttttaat atgcacctga tgcgcctcat taatttattt
     8641 taatacctat caaactcatc ggaaatctga tgagttttta actacagcat ttttacaaac
     8701 taggtaaaat tgtagtataa taaagagaat aggagacaga gtgatgaaac gaaatgtatt
     8761 gattacaggt gcaagtagtg gtattggcaa agcaatcgct tatgtgtttg ctgctaatgg
     8821 tgataatatc atcattacag gacgaagatt ggacaaacta gaagaaatta agcaagatat
     8881 tgaaaagcaa taccatgttc aggtatcagt tcattcattt gatattacga atatggaaca
     8941 aacaattgta aattgtcaaa aaatcttaga agaagttgga caaattcata ttttggtaaa
     9001 caatgctggt ctggcattgg gattagacaa attccaagac tatgatttga ctgacatgat
     9061 gacaatgatt gataccaata ttaaaggatt actaactgtg acacgtcagt tattgccaca
     9121 aatggttgaa aataatgagg ggcatattat caatattgga tcaacggccg gtatttacgc
     9181 ctatgcagga gctgctgtct atgctgcaac caaatcagct gtcaaggtgc tttcagacgg
     9241 gatccgtatt gacacgattg ataaaaatat caaggtaact acgttacaac ccggtatcgt
     9301 tgagactgat tttagccaag tccgttttca tggagataaa gagagagcag cgacagtcta
     9361 tcaagggatt gatgctcttc aagcaaatga tattgccagc tgtactctat ttgttgcaaa
     9421 tcaacctgca catgtgcaga tttctgatat gaccattatg gcaaccaaac aggcaactgg
     9481 ctttactatt catagagatt agaggggaga tagctagaat gaaaagaaaa tatttagcac
     9541 tcgctcttgg cttgctcact atcatatcgc tttctgcctg tcaaactgaa aagaagggca
     9601 gttccgcaag tacctcaact gctgataccc aggtttctaa tgccaataca agcacgtcat
     9661 caacaatcca acgtgagatt tctcttagtt ctatggatat agatgctcta atgcaaggaa
     9721 attacgatag tatactaggc acctggcaaa atagccaagg aaattcacta gtcttcaata
     9781 gcaaaggatt agtagcagat agccagtcct tatttggccg cggaaaaata actgacggaa
     9841 tttttgaaac aggctatgta gacgcaacga ttggcgatgt aacactatta atgattccca
     9901 aaggaacaca agcaaataca actgagatag acactactga ctctacgcgc gatagaatga
     9961 ttgtaataag ccaagaaaca acagcagacg tggtagatgt ctattatcgc ttgaccaatt
    10021 cagccactag cggtatagac ccattaaaaa atactgaaac aggcgtccag ttggatagtg
    10081 gaccaaaaac gattgattat gccaactcaa ttttaggtga aaacaattgg cgcgtcattg
    10141 aaggtaacta cacgcgcacc gaatttattc cttataatat tttggaaggc gatgataatg
    10201 cccgttacac tatttaccaa aacggtgtta ttatcaatgc agattatcaa attgtttatc
    10261 aaccataatg attctctagg aggttagaca tgacattcac atcatttgaa ccaacacgca
    10321 atgttcaaga ttttcatttg gcagcctttg cctactatga tggactagat gtaatcgacc
    10381 aactcaagcc aggaacacct gttcagctag tcggagagcc ttcaaaccca catgactcag
    10441 aagctgtagc aatcttttat caggacacaa agttaggata tatcccatca gataaaaact
    10501 cactaatcag tcggttaatc tattttggac acggcgatat tctagaggca cgtattcaga
    10561 tgtccaatac agaaaatcat cctgatcgcc actttcgagt cgttgtaaaa ctaaaagata
    10621 atcgtaaaaa tgatagtcgg taatttacca acctacccat tttctactta acacggagct
    10681 gtgctctctg tttttgttct tcaataatgc ttaaaaaggc tgcaaactcc ctatccaggc
    10741 gatgttggta ggccttattt tttattgtga gatcttccag gaacactgga taggttcctt
    10801 ttttatacaa ttctccttga aagaagaagc ggtccgataa tttatcaact gaaacaagag
    10861 aagaacgcca gcggctttta cgagaatgag aaggtttcca agatattttt aattgcttct
    10921 cataaaagta ccagcccata atatagattt tagtaatctg atttcgtttt ctgtcttgat
    10981 agaaacaagg aaccgcataa acatttccat gctttgtttg caaaaaagga tggaaatctt
    11041 ttatcttttc gtattctttc tttggaataa gggttaattg attatcctta ataaccttct
    11101 tttcatcaat agtatacgaa taacggtagg ggtcataaat ggttgattgt ccacgcgctc
    11161 tataacgctc tgccatttcc ttaaaaatag gtatacgtcc ataaccatcc atcagactag
    11221 atagactaag tcctcgtcga ttattatttt tcttcacacc aagaatctgg caggtaggcc
    11281 aacaattgat aggaatggct cctaatccga ccagaagacc ggcgctgtca aaatgactgg
    11341 caaagcgtac ctcacgagtg tgaccagtct catttttcaa atcaacaaat acaggttgct
    11401 tgtcctgttc aaaaacaatt atataatttc cagaagactc cgtattttct ctaattggaa
    11461 cggtgtagaa gtggtgaaaa tttggtaatt ctagtaactt tccaagtgtt gatgttttca
    11521 taacacaagt ataacaaaaa atatttttca aatccacaaa aacgtttaat ttgttaaacg
    11581 gaaatataaa gaagctggga aagacatcca tgacgacttt cccactccca ccgtttattc
    11641 aaggataggt gataattgtc ttatctaccg aatcttctaa agcctggcgc gattcttctg
    11701 ataaacgcaa atctgcaagg gtttgttctg aaaaatagta gggatttaga ctatctacaa
    11761 atgcagaaat tgaaatgaca gctttctcca atatcagaga tttttcagtg taagcaaagg
    11821 gaatagcaga ccaatcatca ctctcgaaca tttctaaaat atctttttga acttgaattt
    11881 catcctcatt tttagctgaa actaggattg tttttagtgt agtacgaaaa ctttccttgt
    11941 cttgcaccaa gtcttccaag gtcagcaagc tttcatctgc cttctcataa aaaatatcta
    12001 attccgtctc tgacgaagta gtcacatgga gccagtcagc cttgatactg gttttcatta
    12061 ctgcataggc agaagtattt tcaaagggac tgctgcgcgt ttgaggatag agaacaatcc
    12121 actctttctg gaatagattg tcagggttag ctattttcag ttcctcctca gaagctgtac
    12181 caatccgctg gctgatagca gtagtgtcat cttgctgaaa gaatccaatg atacgcggat
    12241 tttgcggacc gccaatggca tcaatttttg ttcccagatc attaataatg cgtatgttca
    12301 aggcatgaca ctgctcattt aatagtctat tttgctcttt tccgagccag taaattccaa
    12361 tacaagatag accagtggca ataagcaaga caagtaacag cactttcgag tatttcagca
    12421 cagccatcac acctttctaa aacgttttca gtcatatatt atcatatata aatacccgtg
    12481 gtgctagttc atttcaaaat agcgtaagtt atgagctaga ataatttgtt caattcgtaa
    12541 ctgtagtccg gctagacctc tagttaatgt atgttcgatg tcaaagagac gacacagttc
    12601 agaaaaacgt gtttcaatag ttcgtctcat ggccattact ttccaattgt tatgttcttc
    12661 ggaccctgtc atgtttttac gaaacggcgt ccatagatgg taaccttctt gttctaagtc
    12721 cttcttgagt tcactgctca ggtagcctaa atcacctagg ataaccgatt acttacaccc
    12781 ctctaatagc tcataaacca ccttgatgtc atgaacagag gctggcgtga caacatagtt
    12841 cagaatataa ccagataagg tcaccagcat atgaaccttg aagccgtaga accaaagatg
    12901 tttagaggcg ttgtagccaa tatcggctaa tcctttaaaa atggtagctc tgtgattgcg
    12961 aacgggttgg taaagtgaca aaggaaaact atctataatg gctagtttat cagatggaag
    13021 catttcactc atagcctttc agattaattg gacgagccaa atcaattgtt ttgaccgacg
    13081 attgaaacgg cttctttcta gtaactgacc gcaaggaaag aggtggcaga tgctgtagaa
    13141 atggcgctgt gatgtcatac ctagttcagc ttgtaacaaa agtaagacaa ggagtgattc
    13201 atctgaaact ttaactaagc caacattacg tcgatgttta aaggaatccg ggcaatagtt
    13261 gtggtagaag tgatgacaaa tttgtgataa ttggcgtaca ctccattgca aatgatgaga
    13321 tttagcggta tactgtaagt agctcatttg gactaacctc ctgtatgttt cgtcacttac
    13381 agtatagtcc atttgggctt tttattgtat tttgaaatga actagcacca cgggtatata
    13441 aatatcaaaa gcaactattt taaggtacca cattaaacgg ttacacaagt atataaattc
    13501 taagtttacg acaggaatgt ttaatgataa aatataggta aattcgaact cataatttaa
    13561 taggtgtaga tacaacaccg ttggaggata ctatggatga acaaaatcaa ttcaattggg
    13621 taaactttta taaggaatta cccgttgtgc tagttaattc tagctaaata aaaacacttt
    13681 gcgtatcata aactcaaaac aac
//
LOCUS       DATUCG010000061        13397 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.062, whole genome shotgun sequence.
ACCESSION   DATUCG010000061 DATUCG010000000
VERSION     DATUCG010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13397
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.062"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            142..885
                     /locus_tag="VCA20_001406"
     CDS             142..885
                     /locus_tag="VCA20_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1835217.1"
                     /translation="MIQFDHITKTYGETIALDDLNLTIESGEIFGLIGHNGAGKTTTI
                     SLLTSIIEASYGTISVDGLNLEENRDEVKKRIAYVPDSPDIFLHLTAFEYWHFMGKVY
                     GVEKDTVDDRIAKLSSLFDMTARQHEPIDSFSHGMRQKTIIIGALIPNPDIWILDEPL
                     TGLDPQASFDLKQMMKEHAQSGHTVLFSTHVLSVAEQLCDRIAILRKGQLIFVGSLDE
                     LKSQYPDKDLETIYLELAGRQQVKAGEEV"
     gene            882..2492
                     /locus_tag="VCA20_001407"
     CDS             882..2492
                     /locus_tag="VCA20_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEP1835218.1"
                     /translation="MSKSIIWELVKINILYSNPQLLASVKKKQSKRKDASFSAYKSVL
                     LQQVFMVLSFGLLYSVFFMGVDYSQSIGFFSLQLALFTIIAIVYGFTGFFSVFYDSKD
                     TKLYLALPLKPQEVFLAKILSAQGMVLPFLMPCLSLLLITYWQIGGPLLVLASIPSFL
                     ILWILVNLVNLIIMHFVGEVLTKSPRKTLISTILMTGSSLLAFAALMFLQSQQTVSLE
                     SGGFVDFPIIPIFAGFHYLVSHTTSLETLLHFGLPFILMFGLIVFAFKTVIPNYFNQV
                     LAIENASTKRTNNAKSAKIPSNLNQALVKHHLSTLKDSNLMVQTMIQPIIMGVALFPS
                     FSRFTENGGLSSVGWDFFGIALLAGFLLGSLFAGSTTFIGVAMSLEKENYHFIRTLPI
                     SFRKFIIQKFWVLAAVQFALPTLLYFALALGFMKVKFILAVFFTVGIIISALLTGQIY
                     YWRDQRLLMLNWQNSNQLFGRGAGQWLIAGSIIGTMFLGAIILVVSIILAGTIGAAYV
                     SSALMTLLFVLSAALQLYIYKAYWQKLK"
     gene            complement(2665..3084)
                     /locus_tag="VCA20_001408"
     CDS             complement(2665..3084)
                     /locus_tag="VCA20_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEP1835219.1"
                     /translation="MLKAMEVYLVTNGLEAIEFYKNALGATVEQVNLFKDFLPDCPAE
                     LENYVMNAQFRLNGQRFMLSDNNPEMPYTVGYNITVAFITDDAETAQELYSKLSVDAT
                     AINMELQAVPWSPAYGNVTDKFGISLGKLMLKYSGLN"
     gene            complement(3173..3928)
                     /locus_tag="VCA20_001409"
     CDS             complement(3173..3928)
                     /locus_tag="VCA20_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEP1835220.1"
                     /translation="MIMKRTILLCLPAMFLLAACHQNTNQANTTKNSSTTVSTTSSQS
                     ASTTSEQSTSSSTSAETNQEVTSTGTQVNFNGSYYSVQGKYGEVIIANKKHPLAADYA
                     PGENPEALAAFQRLIADMQALGFGISNNYSGFRSYETQASLYQSYVNQEGQAAADRYS
                     ARAGYSEHQTGLAFDLLDTSGNLLEEPNASQWLATNAHLYGFIVRYLPGKESSTGYMA
                     ESWHVRYIGQEATDIYNSGLTLEEYFGVPGGDY"
     gene            complement(4472..4723)
                     /locus_tag="VCA20_001410"
     CDS             complement(4472..4723)
                     /locus_tag="VCA20_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HEP1835221.1"
                     /translation="MKQIKNRLKKHMFWIGLISLVLMLVDNVLFCYFNIECSRTLNQI
                     EQTICIILSILVFLGVLNNKPDVKVEDMMKNGMSSKNQK"
     gene            complement(4967..5731)
                     /locus_tag="VCA20_001411"
     CDS             complement(4967..5731)
                     /locus_tag="VCA20_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEP1835222.1"
                     /translation="MKRLLRRIRPTKPLKELTWIDLIIITTILCGNAIYTSTMQWIAS
                     FSATETVETGVLSFSPADNWWALANQGKLFLFALVYLLIRNYDFKQLKVKLEWRVLLW
                     GPLIFIGAGLISDLAFTAFSYIPGLSGGYNFLGYLPYYDWNIMTVLNRFLAVDYSTVI
                     YSLFNGFYEEFFFLGLLLSTDKKKRSLVLLFSTIVRISFHTYQGMVSALVIGVAFGLF
                     YYYMYTRKNDNLLPYFLGHALADMVGTSFFTLFIAG"
     gene            complement(5810..6493)
                     /locus_tag="VCA20_001412"
     CDS             complement(5810..6493)
                     /locus_tag="VCA20_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HEP1835223.1"
                     /translation="MQYKHLLFDLDHTLLDFSRGEEVALTQFLTAMEVEDIQAFKEVY
                     RPLNQGMWKDLEKGNITKKELINTRFSRTFAHFGRQVDGREMALRYQEFIGRQGQIFT
                     GADKLLQELTHRGYQIYAATNGVTYIQENRLLHSPIQSYFKEVFISEQMGTQKPAADF
                     YEKIAEQIAGFQFDQALMIGDSLTADIQGGNNAGMDTVWYNPTHMINNSKAVPTYTIH
                     SYQELLDLL"
     gene            complement(6736..7491)
                     /locus_tag="VCA20_001413"
     CDS             complement(6736..7491)
                     /locus_tag="VCA20_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor 2"
                     /protein_id="HEP1835224.1"
                     /translation="MSKRKIISSLFLSATVLGGLLFIQTPTVRAIELEDAQTMSTSSA
                     QAPIFSPGELIQETNHTAQFRNSVSIAATNGTSEKEVIWKVDSTYWNFNYDKGGYYFV
                     VPAGMKLKRITLVNGQTREELLDRFPKNVNDNNGSNSSYRLFKRGENTYDNRDFDSQW
                     GWSVGRASNDKINQWKNENAFSDIYYIDSPRHAGPVTYELEAEVTDQTKTSFPLVAVM
                     KNFYARTSYLSEPTSLAGLDVKVEWPQNNNLNL"
     gene            complement(7933..10212)
                     /gene="pcrA"
                     /locus_tag="VCA20_001414"
     CDS             complement(7933..10212)
                     /gene="pcrA"
                     /locus_tag="VCA20_001414"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEP1835225.1"
                     /translation="MNPLLNGMNDRQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKMVNPWNILAITFTNKAAREMKERAYALNPATQDCLIATFHSMCVRILRRDADHI
                     GYNRNFTIVDPGEQRSLMKRILKNLNLDPKKWSERSILGTISNAKNDLIDDKAFEAQA
                     GDLYTQIVAKCYTAYQKELRQSEAVDFDDLIMLTLRLFDQNPEVLTYYQQKFQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPESKVVL
                     LEENYRSTKNVLQAANEVIENNRNRRPKKLWTQNAQGDLITYYRARDENDEAIFVAGQ
                     IDQLHREGKAYKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDVISY
                     LNIIANPSDNISYERIVNEPKRGVGPGTVDKLRDFATSHSMSLLEASQDIMMSPVKGK
                     AAQAVFDLANLLYNLRNQLDNLTVTQVVEAVLNQTGYIDAFAAQNTLEANARIENIQE
                     FLSVTKNFDEKDVEEEETGLDRLTRFLLDLALIADTDDGDTESSEVTLMTLHAAKGLE
                     FPVVFLIGMEENVFPLSRAAEEEDELEEERRLAYVGITRAEQTLYLTNANSRLLFGRT
                     NYNQPSRFIREISSDLLDYQGLARPANTSFEASYVNGRATQFGQGMSLQQALQSRKAS
                     VQPSAKLGGSMPFASSNKSSGSGTNWSVGDIAVHKKWGRGTVLEVSGSGSNQELKINF
                     PEMGLKKVLASLAPISKEE"
     gene            complement(10493..11704)
                     /locus_tag="VCA20_001415"
     CDS             complement(10493..11704)
                     /locus_tag="VCA20_001415"
                     /inference="COORDINATES: protein motif:HMM:NF014691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEP1835226.1"
                     /translation="MYKRLEIVSYSDFDNYEAEYQKRFDSLATIKTGLTINPFSKREQ
                     RRLADKHQLFYVLLPNHVMDIEKIYKNSQLLSNFDLSSDQMQKLFYLQIIDEIQSTND
                     MEGVKSTRKEIKEVYNSLDKNKRFSGIVSMYHSILKNEFEKIDSLSKFRNIYDQLFLD
                     EMSEDKKPDGELFRKKVIYVGDGNSYVHQGNSSEFSINEDLTKLIIFMNNPEIPLIIK
                     SIITHYFFEYVHPFYDGNGRMGRFLMSSYLSRKLDFLTGLSLSESVLQNKSKYEEGFS
                     ITSHPKNRGDLTPFVGTMLEIILNAQENMKTKLGKLKSQLDRVWILIDGLDLTGPQKE
                     LLFILSQDKLFDVLHTGVSNKELTEAFGGKYKRTRIDSTLKTLEGMGLVTKVKSSPVI
                     YEVSSNLIKDI"
     gene            complement(11846..13213)
                     /locus_tag="VCA20_001416"
     CDS             complement(11846..13213)
                     /locus_tag="VCA20_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEP1835227.1"
                     /translation="MAKIVVVGANHAGTAAIKTMLTNYGQENEIVVFDQNSNISFLGC
                     GMALWIGEQIGGPEGLFYSNKEELESLGATVYMGSPVTNIDYDAKTVTALVNGQEHVE
                     SFEKLLFATGSQPILPPIKGAEIKEGSLEFEATLENLQFVKLYQNSADVIEKLKNKDI
                     NRVAVVGAGYIGVELAEAFQRKGKEVILIDVVDTCLAGYYDRDLSDLMAKNLEEHGIK
                     LAFGETVKEVAGNGKVEKIITDKNEYDVDMVILAVGFRPNTALAGEGIERFRNGAFLV
                     NKRQETSIPGVYAIGDCATIYDNATGDTSYIALASNAVRTGIVAAHNICGTDLEGIGV
                     QGSNGISIYGLNLVSTGLTLEKATRLGLNAAVTEVTDNQKPEFMEHGNFPVTIKIVYD
                     KDSRRILGAQMAAREDISMGIHLFSLAIQEGVTIEKLALTDLFFLPHFNKPYNYITVA
                     ALGAE"
BASE COUNT         3919 a         2783 c         2458 g         4237 t
ORIGIN      
        1 atagaaacta ttgcttttgt actaattcag cagtataata aatgtaatta attgtatata
       61 ttattcgaag aacatttgga ataaggcttg gaatacctag caagccccta cttttagttc
      121 acgaataaca aggaggttac tatgatacaa tttgaccata tcacaaaaac atacggcgaa
      181 acaatcgcac ttgatgacct aaatttgaca attgaaagcg gcgagatttt cggtttaatt
      241 ggtcacaatg gggcaggaaa aacaacgaca atcagtctac tgacttcgat aatcgaagcc
      301 agttatggaa ctatctctgt tgatggttta aacctagagg agaaccgcga tgaagtcaag
      361 aaacgaatag cctatgttcc agattcgcca gatattttcc tacacttaac agcttttgaa
      421 tactggcact ttatgggcaa ggtctatggt gtagaaaagg atacagttga tgaccgtatc
      481 gctaaattat ctagtctatt tgatatgaca gcgcgccagc atgagcctat tgatagtttt
      541 tcacacggta tgcgtcagaa aaccattatt attggtgcat tgatccctaa ccctgatatt
      601 tggatcttgg atgaacccct aactggactg gatcctcagg cttctttcga cctaaaacaa
      661 atgatgaaag aacatgctca atctggtcac accgttctat tttctacaca cgtcctttca
      721 gtggctgaac aactctgtga ccgcattgct attctccgaa aagggcaact cattttcgta
      781 ggaagtcttg atgaactcaa atcacagtat ccagataaag atttggagac catctatttg
      841 gaattagctg gacgccaaca agtgaaggca ggtgaggaag tatgagtaaa tccatcattt
      901 gggaattagt aaagattaat attctttatt ccaaccctca acttctagct tccgtcaaga
      961 agaaacaaag taagcgcaaa gacgcctcct tttcagccta taaaagtgtc ctcttgcaac
     1021 aagtatttat ggttttgagt ttcggacttc tctattctgt ctttttcatg ggagtagatt
     1081 atagtcagtc catcggtttc ttttctctgc aactagcgct ctttaccatt attgccattg
     1141 tctacggttt tacaggtttc ttctccgttt tctacgatag taaggataca aaattgtatt
     1201 tagcacttcc cctgaagcca caagaagtct ttctcgctaa aatcctctct gcacaaggga
     1261 tggtcctccc cttcctcatg ccgtgtctgt ctttacttct aatcacttac tggcaaattg
     1321 gcggtccgct cctcgtctta gcttccattc cttccttttt gattctctgg attttagtta
     1381 atttagtcaa tctgattatc atgcactttg ttggggaagt tctgacaaaa agccctcgta
     1441 aaacattgat ttctacaatc ctgatgacag gttcttcctt gctggctttt gctgcgctca
     1501 tgttcctaca atcccagcag accgtttcac tcgaatccgg tggctttgtt gattttccta
     1561 tcatacctat atttgctgga ttccattacc tggtcagcca tactacatca ctagaaacac
     1621 tccttcattt tggacttcct ttcattttga tgtttggact tattgtgttt gcgtttaaaa
     1681 ctgttattcc caactacttt aatcaagttt tggcgattga aaatgcttcg acaaaacgga
     1741 ccaataatgc taagtctgcc aagattccta gcaatctcaa tcaggcttta gtgaaacacc
     1801 acctatctac tcttaaagat agcaacctca tggtccaaac catgatacaa ccaatcatca
     1861 tgggagtggc actttttcca agttttagcc gtttcacgga aaatggtggt ctaagttcag
     1921 ttggatggga tttctttggt attgctctat tagctggatt cttgcttggc agtctatttg
     1981 ctggttctac aaccttcatc ggagtagcca tgtcattgga aaaggaaaat tatcatttca
     2041 ttcgaacgct tcctataagt ttcagaaaat ttattatcca aaaattctgg gtattggctg
     2101 ccgtgcaatt cgcactacca actctacttt actttgcttt ggctctaggt ttcatgaaag
     2161 tcaaattcat tctagcagtc ttctttacag tcggcatcat catctcagcc ctgctaactg
     2221 gtcagattta ttactggcgt gaccaacgac tcctcatgct caactggcaa aatagtaacc
     2281 agttatttgg tcgcggagcg ggtcaatggt tgattgctgg atcgatcata ggaacaatgt
     2341 ttctcggagc cattattcta gttgtcagca tcatattagc tggcactatt ggtgcagctt
     2401 atgtaagctc agccttgatg accttactat ttgttctatc tgctgctctt caactttata
     2461 tctacaaagc ttattggcaa aaactaaaat agattggaca aggccttgtc caatctattt
     2521 tagttttctg cgtaatattc ctgaccgtat ccttctatag tcaagtgaat tagaaacggg
     2581 acatatctaa gtttgtcaat ttgagaactc taaagttatt ttaggagtgg gttattaaca
     2641 ctataactca aggacgtcct tttcctaatt caagccacta tacttcagca ttaatttgcc
     2701 aagagagata ccaaacttgt ctgttacatt accataggca ggagaccaag gaactgcttg
     2761 aagttccata ttgatagcag ttgcatctac ggataacttg ctgtatagtt cctgcgctgt
     2821 ctcagcatca tctgtgataa acgcaactgt aatattgtaa cctactgtat aaggcatttc
     2881 tgggttattg tctgacaaca taaaacgttg accattcaaa cgaaactgtg cattcattac
     2941 gtaattttct agttcagcag ggcagtctgg taagaaatct ttaaatagat ttacttgctc
     3001 aactgttgcc ccaagagcat ttttataaaa ctcgatggct tccaaaccat tagtaaccaa
     3061 gtatacttcc attgctttaa gcatgatgga cctccaaagt tatgatggtt tattatatca
     3121 cgagttagtt atacggacaa attttttaac ttatttctga ctaaatgtaa aattagtagt
     3181 ctccacctgg tacaccaaaa tattcttcca aggtcaagcc tgaattgtaa atatctgtcg
     3241 cttcttgacc aatatatcgc acatgccagg attctgccat atagcctgtg ctagactctt
     3301 ttcctggaag atagcggaca ataaaaccat atagatgtgc attcgtagcc aaccactgag
     3361 aagcattggg ttcttctaat aaattaccac ttgtatccaa taaatcaaag gctaatcctg
     3421 tctgatgttc actatatcca gctcttgcag aatacctatc tgccgctgct tgtccttcct
     3481 gatttacata agactggtag agacttgcct gagtttcata ggaacgaaat ccactatagt
     3541 tattggagat accaaatcct agcgcctgca tatctgctat taatcgttga aaagctgcta
     3601 aggcttcagg attttcgcct ggtgcataat cagctgcaag cggatgcttt ttattggcga
     3661 taatcacttc gccatatttt ccttgtacac tataatagga accgttaaaa tttacctgag
     3721 tccctgtact tgttacttct tgattcgttt ccgccgatgt agagctgctt gttgactgct
     3781 ccgaagttgt tgaagcagac tgacttgaag tagtcgaaac agttgttgac gaattttttg
     3841 tagtattggc ctgatttgta ttttgatgac atgctgctag taaaaacata gccggcaagc
     3901 ataacagaat agtacgtttc attatcatca ctccgttctc ttataattat gtctattgta
     3961 gcacaatcat aagaccccgt ctaatggtgg cttatgattt taaccattaa tattttaatg
     4021 cacgttgaac ttctctagat ttattttctt tataagaatg cacatttacc actgctgcaa
     4081 aaatgataac atacatgtca taaatagaaa cgggagtggg aaagaactcg accaaaataa
     4141 aaagagttcg tcaacaagtc tttatttcta gttgttgagc tgaaacagtc tattcccaga
     4201 ctgtttcact cccacccccg catagctcca acagtcagag tagtgactgt tggaggttgg
     4261 aaatgaagcg aactctgttc gcatcagtcg taatggtcag atttggagtg taaaacacga
     4321 attgctgaga tttgcttcgc aaatcttatc tccaaccttt aacagtccac tggactgtta
     4381 aacccaatca accactgcgc tgagatattg acacgaactc tgagaagagg tgctgggctt
     4441 tctgcccagc ctcaatagtc cctaccaaac attatttttg atttttagat gacataccgt
     4501 ttttcatcat gtcttctacc ttcacatccg gtttattatt tagcactcct aaaaatacaa
     4561 gaatactgag gataatacaa atagtctgct cgatttggtt taaagtacga ctacattcaa
     4621 tattaaaata acaaaatagg acattatcaa cgagcatcaa tactaacgaa atgagtccaa
     4681 tccaaaacat atgttttttt aaacgattct ttatttgttt catatctata ctcctttttg
     4741 atgtataaaa atacatttta acataaataa accgttaatt caaaaaaaat atgggataaa
     4801 aaatatgata aagcaatcga aatttgactg aacagattaa atatatcagc acaataaaac
     4861 gggtgatgat accgagttga tagaaactac gtatcatcat ccgtttgtca gctgtaggga
     4921 gaaagaaatt ttatccgatt agcttgatgt ccgttcttct cattgtttac cctgcaataa
     4981 acaatgtgaa gaaacttgta ccaaccatat ccgcaagagc atgtcctaag aaatatggca
     5041 ataaattatc atttttcctt gtgtacatgt agtaatagaa aaggccgaag gctacaccaa
     5101 taacaagagc agataccatt ccttgatagg tatggaaaga tatacggaca attgttgaaa
     5161 agagtaagac cagcgaacgc tttttcttat ctgtactcaa caataagcct aagaaaaaga
     5221 actcttcata gaagccatta aacaaggaat agataactgt gctgtagtca acggctagga
     5281 aacggttgag cactgtcatg atattccagt catagtaagg caagtatccg aggaaattat
     5341 acccaccaga cagacctgga atatatgaaa aagctgtaaa tgccaaatcg ctaatcagac
     5401 cagcaccgat gaaaatcagt ggtccccaga gcaaaactct ccactctaat tttactttta
     5461 gctgcttgaa atcataattc cgaatcaata aatacaccag agcaaacagg aataattttc
     5521 cttgattggc tagagcccac caattatcgg ctggactaaa cgatagaact cctgtttcaa
     5581 cagtttctgt tgcggaaaat gatgctatcc attgcatggt tgaggtataa atagcattac
     5641 cacataaaat cgttgtgata ataatcaaat ctatccaagt gagttctttt aggggcttgg
     5701 ttggacgaat gcgtctcagt aatcttttca ctttttcctc cttatatttt tgtacatatt
     5761 ctttctgtat aattatatac taaaatcctt ataagtttct taaaatatct tatagcaaat
     5821 ctaataattc ttgatagcta tgaatggtat aggttggaac tgctttactg ttgtttatca
     5881 tatgagtcgg attgtaccaa acagtatcca tgccagcatt attgccgcct tgaatatctg
     5941 ctgtcaaact gtctccaatc attaaggctt gatcaaattg aaaacctgca atctgctctg
     6001 ctattttttc ataaaaatca gccgcaggct tttgcgtccc catctgctca gaaataaata
     6061 cttctttaaa atacgattga atcggtgagt gaagcaagcg attttcctgt atataggtca
     6121 cgccgttggt cgctgcataa atctggtagc ctcggtgtgt caattcctgc aaaagcttat
     6181 ctgcacctgt aaaaatttgt ccctgccgcc caataaattc ttggtagcgc aaggccatct
     6241 cccttccatc aacttgccga ccaaagtggg caaaggtccg tgaaaagcgg gtgtttatta
     6301 gctctttctt tgtaatgttc cctttttcca aatccttcca catgccctga ttgagtggtc
     6361 gataaacttc tttaaaagct tgaatatctt caacctccat agctgtcaaa aactgggtta
     6421 aggctacttc ttctccacgg ctaaaatcaa gaagagtatg gtcaagatca aagagtaagt
     6481 gtttgtattg caagatgata ggtcctttct aagcagaaac tactgaaacg atgtcaggca
     6541 gtatatctaa agtataccac agtttctaaa aaaatagctc cgtgtcctag gctctattat
     6601 actgtttttc agatttcagt agctctggac gtaagggttt cctcatcata atattcaagt
     6661 aaagagaaga ggactcgcag aaaaaggcaa tcgtagtagc cttaactaca cacgattcca
     6721 ctggttggtt tagttctaca aatttaagtt attattttgg ggccattcaa ctttcacatc
     6781 aagtcctgca agcgaagttg gttcactcaa gtatgatgtt ctagcataga agtttttcat
     6841 taccgctacc agagggaagg aagttttagt ttgatcagtt acttctgcct ccaattcata
     6901 agttactgga cctgcatggc gaggactatc gatataatag atgtcagaaa atgcattttc
     6961 atttttccac tgatttatct tatcattact tgctcgtcca actgaccatc cccactgact
     7021 atcgaagtca cgattatcgt aggtattttc ccctctctta aataaacgat aagaactatt
     7081 agaaccatta ttatcattta catttttagg aaaacgatca agcaattcct ctctcgtttg
     7141 accgttaacc agagtaattc gcttcaattt catacctgca ggaacaacaa aatagtatcc
     7201 acctttatca tagttaaagt tccaataagt cgaatcaacc ttccagatta cttctttttc
     7261 agatgttcca ttagttgcag caatagatac agaatttcta aactgagcag tatgattagt
     7321 ctcttgaata agttccccag gagaaaaaat cggtgcttgt gcgctgctag tactcatggt
     7381 ttgtgcatcc tctaactcaa ttgctcttac ggttggtgtc tgtataaaca gcaatcctcc
     7441 caatacggta gcacttaaaa ataaacttga tattatttta cgtttgctca taagagtctc
     7501 cttcttttat ttgatctatt tttgcgctag ctacaaaaat attggcaagc taatcgtccc
     7561 atttctaggc tatcccttcc ttactaagat gagagtctct agcaaataaa agacttttaa
     7621 ctactatcag tataacaata caagtagtat tttctcttgg agctatccgt ttttttctat
     7681 atttgtggtt tagcatatag atggtaagac atactctaaa aacgtccgat tcaaaattca
     7741 aaatttgctt atctagatgt catcaaagcg ggcaagattg tttcgatgct ttaagcagta
     7801 tacaaaaaat cacctcaaaa cagtcaggtc aagactcatt ttgaggtgtc atatatcgtt
     7861 ctaaaaataa tattatgaag acgtcgcaag tcttgtaaaa tgcgatattt cctctttact
     7921 tttgattcat ccctactctt ccttacttat cggtgctaag cttgccaaga cttttttgag
     7981 gcccatttct gggaaattga ttttgagttc ttggttgctg cctgagcctg atacttcaag
     8041 gactgtcccg cgtccccatt tcttgtggac tgcgatgtca ccaactgacc agttggtgcc
     8101 tgagccagac gatttgttac ttgatgcgaa gggcatactg cctccgagct tggcagatgg
     8161 ttgtacggat gcttttcgac tttgtagggc ttgttgcaag ctcatgcctt gtccaaactg
     8221 tgtcgctctg ccattgacat aagaagcctc aaagctagta ttggctggac gagcgaggcc
     8281 ttgataatca agcaagtcac tggaaatttc tcggataaag cggcttggtt ggttgtagtt
     8341 ggtgcgaccg aaaaggagtc gagaattggc attggtcagg taaagagtct gctcagcacg
     8401 ggtgatgcct acgtaagcca aacgacgttc ttcttccaat tcgtcctctt cttctgctgc
     8461 acgactaagt gggaagacat tttcctccat accaatcaag aagacaacag gaaattccag
     8521 acctttggct gcgtggaggg tcataagagt cacttctgaa gattctgtat cgccatcatc
     8581 tgtgtcagca atcagtgcca aatcaagcag gaagcgtgtc aaccgatcca gacctgtctc
     8641 ttcttcctca acatcctttt catcaaagtt tttggtaaca gacaggaatt cctggatgtt
     8701 ttcaatgcgg gcatttgctt ccagggtatt ttgtgctgca aaggcatcga tgtagcctgt
     8761 ttggttcaat actgcctcga ctacttgggt aacagtcaag ttgtctaatt gattacgaag
     8821 attatagagg agattggcta ggtcaaagac tgcctgcgct gccttgccct tgactggtga
     8881 catcataata tcctgagaag cttctagcaa ggacatgctg tggctggtcg caaaatcacg
     8941 gagtttgtca accgtgccag ggccaacgcc acgttttggc tcattgacaa tgcgttcata
     9001 agagatatta tccgatggat ttgcgatgat attcaaataa gaaatcacat ctcgaatttc
     9061 cttacggctg tagaacttgg tgcctccaac catggtataa gggatattag atttgagcag
     9121 ggcttcttca atggtacggg attgggcatt ggtacggtag agaactgcaa aatccttata
     9181 agccttgcct tctcggtgca actggtcaat ctgccctgcg acaaaaatcg cctcgtcatt
     9241 ctcatctctg gcacgatagt aagtaatcaa atccccttga gcattctgtg tccagagttt
     9301 tttgggacgt cgattgcggt tgttttcgat gacctcattg gcagcttgca agacgttctt
     9361 agtcgaacga tagttttcct ccaaaagcac aaccttgctc tctggatagt ccttttcaaa
     9421 gtccaggata ttttgcatat ccgcaccacg ccaaccatag atagactggt ccgcatctcc
     9481 gaccacgcag atattcttga aacgtgatgc caggagcttg actaattggt actgggcatg
     9541 gttggtatcc tgatactcat cgacatggat atactggaat ttctgctggt aataggtcag
     9601 gacctctgga ttttggtcaa aaaggcggag cgtcaacatg atcaaatcat caaagtcaac
     9661 ggcctctgac tgtcttagtt ccttttgata agccgtgtag catttagcaa caatctgcgt
     9721 gtataggtcg cctgcctgcg cttcaaacgc cttgtcatca atcaaatcat ttttggcatt
     9781 ggaaatggtt cccaagatag agcgctcact ccactttttc ggatctaaat taagattttt
     9841 caaaatccgt ttcatgagac tgcgttgctc acctggatcg acaatcgtaa aattacgatt
     9901 atagccgata tggtcagcat ctcggcgtaa aatacggaca cacatggagt ggaaggtagc
     9961 tatcaaacaa tcttgcgtag caggattgag ggcgtaggcc cgctccttca tctcacgcgc
    10021 cgctttgtta gtaaaggtaa tggctaggat attccaagga ttgaccattt tttcatcaat
    10081 caaataagcg atacggtgag tcaggactcg cgtcttaccc gaaccagccc ctgccataat
    10141 caatagcgga ccttccgtcg tctgcactgc ctctgcctgt ctgtcattca ttccgtttaa
    10201 taatgggttc attttctcat actttcttag aacatgtatt acaagtggag tgcttttaaa
    10261 taagagatag tttttcgcta atcaaggcag ttttttgaag gaataccgac tgtattttga
    10321 aaaaaactaa cgatgagtag ctgaaaaact aggcttagat ggaagtacta cactgtgaat
    10381 acaggttcgt tataatcata ccctccatta taccaaaaaa tctctcgata ggcagttcaa
    10441 tgtgatagat ggcaagcaaa aaagcctaaa agcccagccg aggccggtgc tattaaatat
    10501 cttttatcaa attagaacta acttcataaa tgactgggga tgatttcact tttgtcacta
    10561 agcccattcc ttccagtgtt ttcagagtag aatcaattct cgtccttttg tattttccgc
    10621 caaatgcttc cgttaattcc ttgttgctga cgccagtatg caaaacatca aaaagcttat
    10681 cttgggaaag tataaacaac aattcttttt gaggtcccgt caggtctaga ccatctatca
    10741 agatccacac tctatctaat tgtgacttca acttgcccaa cttagtcttc atgttctctt
    10801 gtgcatttaa aatgatttct agcatggtcc ctacaaacgg ggtcaaatca cctctatttt
    10861 tagggtgaga agtaattgag aaaccctcct catacttact cttattttgc aaaacagact
    10921 ccgacagaga caaccctgtt aaaaaatcta attttctaga cagataggat gacattagga
    10981 aacgccccat tctaccattt ccatcataga agggatgtac atactcaaaa aaatagtgcg
    11041 taataattga tttgataata agaggaatct ccggattgtt cataaaaatg attaacttcg
    11101 ttaaatcttc attaatcgaa aactccgagg aattcccttg gtgaacatag gaatttccat
    11161 ctccgacata aatcactttt tttctaaaaa gctctccatc aggcttttta tcttctgaca
    11221 tctcatctaa aaataattga tcataaatat tcctaaattt ggacaaagaa tctattttct
    11281 caaattcatt tttcaaaata gaatggtaca ttgaaacgat tccagaaaac cttttatttt
    11341 tatctagaga gttatatacc tcctttattt ctttacgagt agatttgact ccttccatat
    11401 cattggtact ttggatttca tcaatgattt gcaaatagaa tagcttttgc atctgatcgc
    11461 ttgataaatc aaaattagaa agaagttggc tatttttata aatcttttca atatccatga
    11521 catggttcgg aagcaataca tagaacaatt ggtgtttatc tgccaacctt ctttgttccc
    11581 tctttgaaaa aggattgatt gtcaatcctg tttttatggt agctagacta tcaaagcgtt
    11641 tttgatattc cgcttcataa ttatcaaaat cagaataact cacaatttct agtcttttgt
    11701 acataaaaac acctcttttg tttttgaatt tctaaaattt taccattttt taaaattcaa
    11761 ttcaggtttt ttgatatttt taaatataat ttcaaatttt actaaaaaag cccagagtca
    11821 tctctgagct tttgtattca attgattatt cagcacccaa ggcagctact gtgatgtagt
    11881 tgtaaggttt gttgaagtgt ggcaagaaga acaagtcagt caatgccaat ttctcgattg
    11941 ttacgccttc ttggattgcc aatgagaaga ggtggatacc cattgagata tcttcacgag
    12001 ccgccatttg cgcaccaagg atacgacgtg aatctttatc gtaaacaatc ttgatagtaa
    12061 cagggaagtt gccgtgctcc atgaattctg gtttttgatt gtctgttact tcagtaacag
    12121 cagcgttcaa accaagacga gtagcttttt caagtgtcaa accagttgaa acaaggttaa
    12181 gaccgtagat agagataccg ttagaacctt gtacaccgat accttcaaga tcagtaccac
    12241 agatgttgtg agctgctacg ataccagtac gtactgcgtt tgaagccaaa gcgatgtagc
    12301 ttgtgtcgcc agtagcgttg tcatagatag ttgcacagtc accgatagca taaacgcctg
    12361 ggatagaagt ttcttggcgt ttgtttacaa ggaaggcacc gttacggaaa cgctcgattc
    12421 cttcacctgc aagcgcagtg tttggacgga aaccaacagc caagataacc atgtcaacat
    12481 cgtattcgtt cttgtcagtg atgattttct caactttacc attaccagca acttctttaa
    12541 cagtttcgcc gaaggcaagt ttgataccat gctcttctaa gtttttagcc ataaggtctg
    12601 acaagtcacg gtcatagtaa ccagccaaac atgtatccac aacgtcaata aggataactt
    12661 ctttaccttt acgttggaat gcttccgcaa gctcaacacc gatgtagcca gcaccaacaa
    12721 ctgcgacgcg gttgatgtct ttatttttca atttttcaat aacatctgct gagttttggt
    12781 acaatttaac aaattgcaag ttttcaagag ttgcttcaaa ttcaagagaa ccttctttga
    12841 tttcagcacc tttgattggt ggcaagatag gttgtgaacc agttgcgaaa agaagttttt
    12901 caaatgattc aacatgttct tgaccattta caagcgctgt aactgttttg gcatcatagt
    12961 caatgtttgt aacaggtgac cccatgtata ctgtcgcgcc caagctctcc aactcttctt
    13021 tgtttgagta gaaaagtcct tcaggaccgc caatttgctc accgatccac aaagccatac
    13081 cacaacccaa gaatgaaata tttgagtttt ggtcaaatac aacgatttcg ttttcttgac
    13141 cataatttgt caacatagtt ttgattgcgg cagtaccagc atggttagca ccgacaacaa
    13201 cgattttagc cataatgtaa aatctcactt tcgaaaatat atttttttac gaactatatt
    13261 atacaatata aatgttaccg atttcaaaaa atatgctcag aatatcacat ttttttcaaa
    13321 aagtttttga aatgctgttt tctttaaaca atgtaaagat atataaaagc aaaaaaccta
    13381 gatttctcta ggtttct
//
LOCUS       DATUCG010000062        13332 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.063, whole genome shotgun sequence.
ACCESSION   DATUCG010000062 DATUCG010000000
VERSION     DATUCG010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13332
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.063"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(19..567)
                     /locus_tag="VCA20_001417"
     CDS             complement(19..567)
                     /locus_tag="VCA20_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835228.1"
                     /translation="MLTEEQLQDIFELADLMSNGDRELFLQLRDVVFASDPNQILDSI
                     ERILEPDSFDDFLDRVGESEKENLWLILIKLLEHFNYICVRDYKDNLEDFIYFFDRLH
                     QVRNAGISLKLDSDGLNPAASISEWARVVDSKYLDENFCLGAVDMDTDSYYLFFSKQA
                     TFVRLQELAGNLGYRIDYAKNM"
     gene            complement(578..1021)
                     /locus_tag="VCA20_001418"
     CDS             complement(578..1021)
                     /locus_tag="VCA20_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835229.1"
                     /translation="MISYKRSIVIPSVISLVFILLGLGGLTRPEPSMGSVVYLIFLQP
                     LLVYLYILRKQKAVFILTKQYLEFQEHFWSERQRHSLEEIAEIRYMVSPVYRGYQSKR
                     LRIVGNKGALLAVVNLNKLDGADFNDIYHFVEQVAPHIRWDFSNS"
     gene            complement(1023..2825)
                     /gene="pepF"
                     /locus_tag="VCA20_001419"
     CDS             complement(1023..2825)
                     /gene="pepF"
                     /locus_tag="VCA20_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEP1835230.1"
                     /translation="MSKQRYEIEEKYQWDLTTIFPTDDAWEAELADLQAETEKTKEFA
                     GHLLDSAKSLLEISETQLGLMRRIEKLYVYASMKNDQDTREGKYQEFQAKAMGLYAAF
                     SQAFAFYEPEFMAITEEQLEAFKAEEPTLVQYSHQFEKLLAAKDHVLSQEVEEVLAAT
                     SEIFESPAETFSVLDNASLRFPEIADEDGHLVPLSHGNYIHFMESKNREVRQEAYEAM
                     YGTYEQFQHTYAKTLQSNVKVNNLKARLRKYDSARHASLAANFIPESVYDTLVAAVNK
                     HLPLLHRYINLRKKLLGIEDLKMYDMYTPLSDFSTKISYEDALKKCQETLEIFGEDYS
                     AIVKEAFENRWIDVHVNEGKRSGAYSGGAYDTNAFMLLNWQDNLDNMYTLIHETGHSL
                     HSMLTRKTQPYVYGHYSLFLAEIASTTNENLLTEKLLAEVEDDKERFAILNHYLDGFR
                     GTVFRQTQFAEFEQAIYKADQEGQVLTADFLNELYGQTNEKYYGLSAEENPEIQFEWA
                     RIPHFYYNFYVYQYATGFAAASALANKIVNGSPEDKENYLNYLKAGSSDYSLNVIAKA
                     GVDMTKEDYLNDAFKVFEARLEELEALVEKGVHL"
     gene            complement(2898..3524)
                     /locus_tag="VCA20_001420"
     CDS             complement(2898..3524)
                     /locus_tag="VCA20_001420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835231.1"
                     /translation="MKFSRFTLVSASLLLLFSLSACAVSHEEGQTTTASSQQVQTINE
                     EEAQKFTSQHSQTVASKEVAEGGGTLVTFEDGTEYLFTEEYYVLSYQSGCKVTYFVES
                     GDYTVATDEYEGEDKASEAKGQVGQYFVESGYSISFANDGTLLIDLQGNFTHQYDGKE
                     KKGNVKEAEGSSYVDSLLNQYGIPNLAFYDSFVKEAVVDAKTYFTADE"
     gene            complement(3569..4510)
                     /locus_tag="VCA20_001421"
     CDS             complement(3569..4510)
                     /locus_tag="VCA20_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEP1835232.1"
                     /translation="MLVALDEDGQVFNVLENPAPQGRYSCPGCGGPVRYKSGKVLRSH
                     FAHVTLRDCTYFSENESAQHLSLKSSLYKWLQGQEEVELEAYLPATKQVADILVNQHL
                     ALEVQCSSLSISRLQERTMAYQQADMAVLWLLGKDLWLGERLTNLHKQFLYFSMNMGF
                     HLWELDDEKKELRLRYLIHEDLRGKVHCLTKAFPFGQGNLLDILRLPFAKQALSYFTC
                     SVDQDLLDYVAKQLYYRVPKWMNLQAEAYQQGKNLLSQSLDDFYPQIRLPRSAIGFAQ
                     IKQDLSSLYQSFDAYYAKVENKGKQVLYPPIMYRQLM"
     gene            complement(4571..6574)
                     /gene="metG"
                     /locus_tag="VCA20_001422"
     CDS             complement(4571..6574)
                     /gene="metG"
                     /locus_tag="VCA20_001422"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019313364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEP1835233.1"
                     /translation="MTKTPFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGHEV
                     FYLTGLDEHGQKIEEKAKEAGLTPQAYVDGMAVGVKELWNLLDISYDKFIRTTDDYHE
                     EVVAAVFEKLLAQDDIYLGEYSGWYSVSDEEFFTESQLEEVFRDENGKVTGGIAPSGH
                     EVAWVSEESYFLRLSKYQDKLVEFFNAHPDFIQPDGRLNEIMKNFIEPGLEDLAVSRT
                     SFTWGVPVPSNPKHVVYVWIDALLNYATALGYGQEETANYDKFWNGTVYHMVGKDILR
                     FHSIYWPIMLMMLDMKLPDRLVAHGWFVMKDGKMSKSKGNVVYPEMLVERFGLDSLRY
                     YLMRSLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVAMINKYFGGQVPTFVANVT
                     DFDADLAAVVADNLASYHKYMEAVDYPRALEAVWNIISRTNKYIDETAPWILAKEEAD
                     RDKLAAVMAHLTAGLRVVAHLIQPFMMTTSNAIMEQLGLGTEFDLENLDFAGLPAGLT
                     VVEKGTPIFPRLDMEEEIAYIQAQMGGSSAISQEEEKEWNPADVELKNEKAAIKFEDF
                     DAVEIRVAEVKEVAKVEGSDKLLKFRLDAGDGQDRQILSGIAKYYPNEQELVGKKVQI
                     VANLKPRKMMGLLSQGMILSAEHGGNLTLLTVDPSVPNGSQIG"
     gene            complement(6783..7531)
                     /locus_tag="VCA20_001423"
                     /pseudo
     CDS             complement(6783..7531)
                     /locus_tag="VCA20_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298997.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7873..10971)
                     /locus_tag="VCA20_001424"
     CDS             complement(7873..10971)
                     /locus_tag="VCA20_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEP1835234.1"
                     /translation="MKLQFKQQQFQLDAVASVVDVFAGQGKHSGFDYHMDKGQGMGAE
                     LDMNFIGFRNAPIQLSQDTISQQVRTQQLRHGLKPSENLSIQKVDGRPSYNLTIEMET
                     GTGKTYTYIRTLMELNKQYGWLKFIIVVPSIAIREGVLKSFEIMADHFQMEYGKKPRY
                     FVYDSSRLGELDKFANSSDIQVMIINSQAFNATGADARRIHTEQESFRWRKPIDVIAA
                     TNPILIIDEPQSVEGKKTKERLEDFKPLFTLRYSATHKDKHDMIYRLDALDAYNKKLV
                     KKIAVKTVEQTSTTGTQGYLYLQELVPQKSGAPKARIEFEMRTKSSDVKRVTKLVEEP
                     FALYEESGNLPSYQDGWTLSHFDAREGENSIQIGANRKLHVGEVIGETNEDDIRRIQI
                     RETIASHLQKEEGLYKRGIKVLSLFFIDEVAKYKCYDEQNDAYNGTYAQIFEEEYEAQ
                     VKALLADPNLHGSNFWHYLNNHQKGNPVHAGYFSVDKVKKSDKVKFVDYKSATEKRNN
                     QSNDKDAFDLIMKDKERLLSFDEPVRFIFSHSALKEGWDNPNVFQICTLKNTGTETEK
                     RQKIGRGMRLAVDQKGVRQDEELLGADVHNINKLTVIANESYEDFAKNLQRELREILA
                     DRPSKVEVDLFVDKVLENEAGQKLTVTKEVAKKIVHQLIQQKYIDGEDNLTATYFEQK
                     ETSQFVLSENLAGYEVAVKDILESIYDANRYQIERENDSEVVFAREINPDNYNRQEFK
                     RLWESIHHKSTYYVEFDDEELIEKSVKVINEALFVKKPSYMITEAEAREMKAEQGLDF
                     KVNKDGKRQAELDKSSIQLRYDLLGEIASRTELKRKTVANILKQISPSKFDMFSYNPE
                     AFIQRTSRLINEQKASQVIEHIRYNILEETFDTSVFTDNPESGKLSDKRILESQKGVY
                     NYVKVDSDTERKFKEELDQFEDVMVYTKLPSNFKISTPLGAYNPDWAIAFRDGSVKHV
                     YFVAETKGSMSSLQLKGAELAKIECAKAHFKALKEKGLIADDHMYDVVDSYEKLLEIV
                     RG"
     gene            complement(10968..11414)
                     /locus_tag="VCA20_001425"
     CDS             complement(10968..11414)
                     /locus_tag="VCA20_001425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835235.1"
                     /translation="MMELGSVFEGLSIPFGLITALLGYLLGNKTKFYEEKITIYSELM
                     YLLDDVLNIRKLSVEETTKLYTELNKLIAKSYFFTNKIVYQQLLDFIKSFEEYGNNKS
                     VGTSRVLYEAKTLAEVLKIDSQAFRVLTKTKVLRLKDRFHKEGGNQ"
     gene            complement(11416..13191)
                     /locus_tag="VCA20_001426"
     CDS             complement(11416..13191)
                     /locus_tag="VCA20_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004244930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEP1835236.1"
                     /translation="MDFDKLRQFLSREIVEGRESYEFTWVGKRGAIAEAGKPSLKTLR
                     PDLEESVDFDKSENVFITGDNLEVLKVLQESYLGKIDMIYIDPPYNTGKDFVYSDKFQ
                     MSEEELADEMDLRDENGLQRVGLTKNEKSSARYHSDWLNMMYPRLVLARNLLKDSGVI
                     FISIDDNEQSNLKAICDEIFGEENFRNSIATRRYDKNLNRQFMAEGLHSMNVGLEYIF
                     VYSKSQISRFNPVFKESSEVRSQSGYWKGFWNNADRPTMRYEILGYTPDEGQWKWKKE
                     LAEEAIHNYQVYLENFSETMSIEEYWKHTGSTKKFLRRNLNGNGKNKGVEHWVAPSDG
                     ILRNSNWSDMLISKPTGLEIPFDSPKNPKVIQELIKFTSSERDINILDFFAGSATTAD
                     AVMQLNAEDGGNRKYILCTLDEQVADKAAAKEAGYETIDQISRERIRRAAKKIQEEHP
                     ETVGKQDFGFRAYKLDSSNFKDVSQTPDNFSQESLFDSVSNIKDGRTDLDLLFQIMLI
                     WGMELSLPIAKTQIDGTAVYNVADGALIACFADEISESVIRQIAKEEPLRAVFKDESF
                     TNSAAKINLGQIFKEEAPNTKVKVV"
BASE COUNT         3629 a         3077 c         2615 g         4011 t
ORIGIN      
        1 ggtttcccca ccttttcttt acatattttt ggcgtaatca attcgatacc ctaaatttcc
       61 agctagttct tgaagtcgaa caaaagtcgc ttgcttactg aaaaagagat agtaactatc
      121 cgtgtccata tctactgccc ctagacaaaa gttttcatca agatatttgc tatcaacaac
      181 tcttgcccat tctgaaatag aggcagctgg atttaatcca tctgaatcca atttgagaga
      241 aatccctgca ttgcggactt gatgaagacg gtcaaaaaag tagataaaat cttcaagatt
      301 atccttataa tcacgcacac aaatataatt aaaatgctct agtaatttta tcaagattaa
      361 ccaaagattt tccttttcag actccccaac acggtccaag aaatcatcga atgaatctgg
      421 ttctaggatg cgttcaatag agtctaaaat ctgattcgga tcactagcaa aaaccacatc
      481 cctaagttga agaaatagct ccctgtcacc atttgacatg aggtctgcca gttcaaaaat
      541 gtcctgtaat tgttcttccg ttaacataat ccctccatta tgaatttgaa aaatcccatc
      601 ttatatgcgg tgctacttgc tctacaaaat ggtaaatatc attaaaatct gcaccatcaa
      661 gcttattcaa attgacgact gcaagtagcg ctcccttatt tcctacgatt cgcaaacgtt
      721 tcgactgata gccacgatag acaggagaaa ccatgtatcg aatctccgca atctcttcta
      781 acgaatggcg ctggcgttca ctccaaaagt gttcttgaaa ttctaggtac tgttttgtca
      841 agataaaaac agccttttgt tttctcaata tatataaata aaccagcaaa ggttgtaaaa
      901 atatcaaata gacgacactt cccatagatg gttcaggtct agtcaaacca ccaagaccga
      961 gcaagatgaa aacaagacta atcacactgg gaatgacaat cgaacgttta taggaaatca
     1021 tattagaggt gcacaccttt ctcaacgaga gcttctaatt cttccaaacg agcttcaaat
     1081 accttgaagg cgtcgttgag gtaatcttcc ttggtcatgt ctacgccagc cttagcaatg
     1141 acattgaggg agtaatctga gctacctgct ttcaagtagt tgagatagtt ttctttgtct
     1201 tctgggctac cattgacaat cttgtttgct agagctgatg ctgctgcgaa acctgtcgca
     1261 tactggtaga catagaaatt gtaatagaag tgtggaatac gagcccactc aaactggatt
     1321 tctggatttt cttctgcaga aagtccgtag tatttctcgt ttgtctgacc gtacaattca
     1381 ttgaggaaat cagctgtcaa gacctgacct tcctggtctg ccttgtaaat agcttgttca
     1441 aactcggcaa attgtgtctg gcggaagact gtaccacgga aaccatctag gtaatggtta
     1501 agaatagcaa agcgttcctt gtcatcttct acttctgcca agagtttttc tgtcaaaaga
     1561 ttttcattgg ttgttgacgc aatttctgcc aagaaaagtg aataatgacc atagacatag
     1621 ggttgagtct tacgagtcaa catagagtga agagaatgcc ctgtttcatg gataagggtg
     1681 tacatattat ccaaattatc ctgccaattg agaagcatga aggcgttggt gtcgtaggca
     1741 cctcctgaat atgcacctga acgttttcct tcattaacat gaacatcaat ccagcgattt
     1801 tcaaaagctt ctttaacgat agccgaataa tcctcaccaa agatttccaa ggtttcttgg
     1861 cattttttaa gagcatcttc ataagaaatc ttggtactaa aatctgacaa tggtgtgtac
     1921 atatcgtaca tcttcaagtc ctcaatacct aggagtttct tacgcaaatt gatataacgg
     1981 tgcaagagcg gaaggtgctt gttgactgcc gccactaatg tatcgtaaac agattctgga
     2041 atgaaattag ctgcaagtga tgcgtgacga gctgagtcgt acttccgcaa acgggctttc
     2101 aggttattga ctttgacatt agattgcaga gtcttagcgt aggtatgctg gaactgctca
     2161 taggtaccgt acatagcctc ataggcttct tgacggactt cacggttttt agactccata
     2221 aagtggatgt agttcccgtg tgaaagtggc accaagtgac cgtcttcatc tgcaatttct
     2281 gggaaacgca gactggcatt gtccaagact gagaaggttt cagcaggtga ttcaaaaatc
     2341 tcgctggtcg ccgcaaggac ttcttctacc tcttgtgaca ggacatggtc cttggctgcc
     2401 agaagctttt caaattggtg gctgtactga acaagagtgg gctcttctgc cttaaaggcc
     2461 tccagctgct cttcagtaat ggccataaat tcaggctcat agaaagcaaa agcctgtgaa
     2521 aaggcagcat acaagcccat ggccttagct tgaaattcct gatatttgcc ttcacgagta
     2581 tcttggtcgt tcttcataga cgcatagaca tagagttttt caatgcgacg catgagaccg
     2641 agttgcgttt cgctaatttc taagagactt ttagctgagt ccagcaaatg acctgcaaat
     2701 tcttttgttt tttcagtttc agcttgtaaa tctgcaagtt ctgcctccca ggcatcatcg
     2761 gttgggaaaa tagtggtcaa gtcccactgg tatttttctt caatttcgta acgttgttta
     2821 gacataatta atcctccgta tcctttattc taacataaaa aatcccctct ttccaacgga
     2881 aagtgagggc ttacaagtta ctcatcagca gtaaagtagg tcttggcatc tactactgct
     2941 tctttgacaa aactgtcata aaaggcaagg ttagggatgc catattggtt taacaagcta
     3001 tcgacgtaac tactcccttc tgcctcttta acatttcctt ttttctcttt accatcatac
     3061 tggtgagtga aatttccttg taaatcaatg aggagggtgc catcatttgc aaaagaaata
     3121 gagtagccac tttctacaaa atactgtcca acctgcccct tagcttcact tgctttgtct
     3181 tctccttcgt attcatctgt cgctacagta tagtcaccgc tttcgacgaa ataggttacc
     3241 ttacaaccac tttggtagga aagcacataa tattcttcag taaaaaggta ttccgtacca
     3301 tcttcaaaag taacgagtgt ccctccaccc tctgctactt ctttagaggc tactgtctga
     3361 ctatgctggg aggtaaactt ctgtgcttct tcctcattga ttgtttgcac ttgctgactg
     3421 gaggctgtgg tggtttgccc ctcctcatga cttacagcac aggccgataa gctaaacaat
     3481 agtagcaaag aagcacttac taaggtaaaa cgactaaatt tcataccacg aacctccttg
     3541 taaacctttt cttttattat atcattattt acataagttg tctatacatt ataggtggat
     3601 ataggacttg ttttcctttg ttctctacct tggcataata ggcatcaaaa gattggtaga
     3661 gagaagatag gtcttgtttg atttgggcaa agccaatggc agagcggggc agacggattt
     3721 ggggatagaa gtcatccaag gactgactga gcaagttttt cccttgttgg taggcctccg
     3781 cctgcaagtt catccacttg ggaactcggt aatagagttg cttggcaaca tagtccagca
     3841 aatcctgatc aaccgaacaa gtaaaatagg acaaggcttg cttagcaaag ggaagccgta
     3901 aaatatctag taaattccct tgaccaaagg gaaaggcctt ggtcagacag tggaccttac
     3961 ctcgtaagtc ttcgtgaata aggtaacgaa gacgcaattc cttcttctca tcatccaatt
     4021 cccagagatg aaaacccatg ttcatgctaa aatacagaaa ttgtttgtgg agattggtca
     4081 gccgttctcc aagccagaga tccttgccca agagccacaa aacagccata tctgcttgtt
     4141 ggtaggccat agtccgttcc tgcaagcgtg aaatagacag gctagagcac tggacttcta
     4201 gtgctagatg ctgattgact aaaatgtctg ccacctgctt ggttgcagga agataggcct
     4261 ctaactctac ttcttcctga ccctgtaacc acttatacaa actggacttg agggacagat
     4321 gctgtgcgga ctcattctcg gagaagtatg tacaatcccg aagggtgaca tgggcaaaat
     4381 gcgaacgcag aacttttccg gatttgtatc ggaccggtcc tccacaacct ggacaagaat
     4441 agcgaccttg cggcgcagga ttttccaaga cattaaaaac ttgtccatct tcatcaagtg
     4501 caactaacat agaacctcct ctctttatta gttcgcaaaa aaacacccag ctgtgctgag
     4561 tgttatgaat ttacccaatc tgactgccgt ttggcacaga tggatcgact gttaagagcg
     4621 ttaaattgcc gccgtgttcg gctgagagga tcatgccctg gctgagcaaa cccatcatct
     4681 tacgtggctt gaggttggca acgatttgaa ctttcttgcc gaccaactcc tgctcatttg
     4741 gatagtactt ggcgatacct gacaggattt ggcggtcttg gccatcacct gcgtccagac
     4801 ggaacttgag caacttgtca gaaccctcca ctttggctac ttccttgact tcagccacgc
     4861 ggatttcaac cgcatcaaag tcctcgaact tgattgcggc cttctcgttt ttgagttcca
     4921 catcagctgg gttccactcc ttttcttcct cttgggaaat agcagagcta ccacccattt
     4981 gggcttggat ataggcgatt tcctcttcca tatccagacg tgggaagata ggcgttcctt
     5041 tttctaccac tgtcaaacca gctggaagac ctgcaaaatc gagattttca aggtcaaatt
     5101 ctgtacccaa accaagctgt tccataatag catttgaagt cgtcatcata aatggttgaa
     5161 tgaggtgagc caccacacgc agacctgctg ttaagtgagc catgactgct gccaacttgt
     5221 cacggtctgc ctcctctttt gccaatatcc acggtgctgt ttcatcgatg tacttgttgg
     5281 tacgggagat gatattccag accgcttcca gagcgcgtgg atagtcaaca gcttccatat
     5341 acttgtggta gcttgccaag ttgtcagcta caactgccgc caagtccgca tcaaagtccg
     5401 tcacattagc cacaaaagtc ggtacctgac caccgaaata cttgttaatc atagcaaccg
     5461 tacggttgag caagtttcca aggtcgttgg ccagttcgta gttaatacga ccaacataat
     5521 cttctggtgt aaaggtgccg tcagatccaa ctggcagact acgcatgagg tagtaacgga
     5581 gtgaatccaa gccgaaacgc tcgaccagca tttctggata gaccacatta cccttagact
     5641 tggacatctt gccatctttc atgacaaacc agccatgagc aaccaagcgg tctggcaact
     5701 tcatatccaa catcatgagc atgattggcc agtaaatgga gtggaaacga agaatgtcct
     5761 tgccaaccat gtggtaaact gttccgttcc agaacttgtc atagttggct gtttcttcct
     5821 gaccataacc caaggcagtt gcgtagttga gtagggcatc gatccagaca tagacaacgt
     5881 gtttaggatt ggacggtaca ggtacacccc aggtaaatga ggtacgtgac actgccaaat
     5941 cttctaagcc tggctcgatg aagtttttca tgatttcatt gagacgaccg tccggctgga
     6001 taaagtctgg gtgagcattg aagaactcaa ccaatttatc ctgatacttg ctgaggcgga
     6061 ggaagtagga ttcttctgaa acccaggcaa cctcgtgacc gctcggagcg ataccacctg
     6121 ttaccttacc attttcatct cgaaagacct cttctaattg gctctcggtg aaaaattcct
     6181 cgtctgatac ggagtaccat ccagaatact ctcccaagta aatgtcgtct tgcgccagca
     6241 atttttcaaa aacagccgcc accacttcct cgtggtaatc atcagtcgtg cggataaatt
     6301 tgtcgtaaga gatgtccagc aaattccaga gctccttgac accaaccgcc ataccgtcaa
     6361 cgtaggcttg cggagtcaaa ccagcttctt ttgccttctc ttcaatcttc tggccgtgct
     6421 catcaagccc tgtcagataa aaaacctcgt ggcccatcag gcgtttgtaa cgagccaata
     6481 catcgcaggc aatggtcgtg taggctgaac cgatgtgaag ttttccagac ggataataaa
     6541 tcggtgtggt aatgtaaaac ggtgtttttg tcatgttttt ttcctttcaa attcagagca
     6601 aatgaaaccc tgttattgac aacatcctat tttatcatat tttcagctaa atcacaaagc
     6661 ggttctagat acaagccgag tagaaagcta atgtctatct tctctataaa attatagttc
     6721 tgttcttgtc aattgacgaa gagcgaaaaa ccgaaaccag tttggaactg tttccggtgt
     6781 tcttatacaa tatggagctg ttcaaataca tttttcaaat cagctatcat ttctcgatga
     6841 aaatccaaat aataggtttt ctggtcagaa tctggtaatt tttcaatatc agctaagatt
     6901 gcttgataac gatggagaat tttcttacca gcttctttgt ctatctcgtc aattttacca
     6961 atacctactg ctatttcatc caaactgtca tattttgcaa gtacatcctc tgtccactca
     7021 cgcttgtctt tgttaagaat gacaatcttt tctgtcagcc agtcttcata actttcctct
     7081 tctgtcacta gcgactggct cgcagtagac ttaggcactt ggacaattct gcttgaacga
     7141 aggtaattcc aatagaacca gactgcaaat aggatggcta gaatgatatt gattgggaaa
     7201 acaaagctga gggcgaagag cacggtcagc cacaaaatca acttcacatt tcttgcgttt
     7261 ttctctgctc gcctgttctt ctcttcccaa tcatccaata tagactgggc ttgggcagta
     7321 taggtttcaa ccaaatttgc cagcgtaact ttttctctgg atcattttcc agttccattg
     7381 ccagataggc ttggttggca cgagccttgg ctgcctcata ggtctggaca gtcgccaaat
     7441 attccgcact gctattcatc tgattcagca agcggtccgt ctgccctttg ataaaagact
     7501 gatctcttga tgttaattta cggtaaggca taacaacctc ctcctgaata tctgaaatat
     7561 actaccattt tacgataggt tacattcttt tgcaatgctt caatatcatt atttccttct
     7621 gccaaacaat aggcttaaaa tgacaaaacc acctgccaat gcgacaataa ttacaggtga
     7681 ggcccaaagc aagattaaaa accaaatcaa gagtttcata aagagttctt ccatttgtct
     7741 ttcctcctac ttcctacgtc tgagaccaat cgttatagcg ataatgccta ccagataaca
     7801 aatcgcaaat gtaaaaaata gaaatgcacc catatatttc tccttctctt gtgataagga
     7861 ttgcttggtt gcttatcccc tcacgatttc taataatttt tcataggaat ccaccacatc
     7921 gtacatgtgg tcgtccgcaa tcaaaccttt ttctttcagg gccttgaagt gggccttggc
     7981 acactcaatt tttgcaagct ctgccccttt cagttgcaaa ctagacatgg aacccttggt
     8041 ttcagctaca aagtaaacat gcttaaccga accatctcga aaggcaattg cccagtcagg
     8101 gttgtaagca cccagagggg tggaaatctt aaaattgcta ggtaatttgg tgtagaccat
     8161 gacatcttca aattgatcca actcttcttt gaacttccgc tccgtatccg aatcaacctt
     8221 tacataatta tacacgcctt tttgcgattc cagtatccgt ttgtccgaca attttccgct
     8281 ttctgggtta tccgtaaaaa cagaagtgtc aaaggtttct tccaagatat tgtagcggat
     8341 atgctcaatc acctgactag ccttttgttc attgatcagg cgacttgttc gttggataaa
     8401 tgcctcaggg ttatagctga acatgtcaaa tttgcttggt gagatctgtt tcaggatatt
     8461 tgcaactgtt ttgcgtttca actccgtgcg actggcgatt tcacccaaca aatcatagcg
     8521 gagttgaata gaagacttgt ccaattcagc ctgacgcttg ccgtctttat tgaccttgaa
     8581 atccaaacct tgttcagcct tcatttccct tgcttcagcc tctgtaatca tatagcttgg
     8641 ctttttaaca aagagagcct cattgataac cttgaccgat ttttcaatca actcctcatc
     8701 gtcaaactcc acataatagg tggacttgtg atgaatgctt tcccacagac gtttaaactc
     8761 ttgacggttg tagttgtcag gattgatttc tctagcaaag acaacttccg aatcattttc
     8821 acgctcaatc tggtaacgat tggcatcgta aatagactcc aagatgtctt ttacagccac
     8881 ttcatagcca gccagatttt cagatagaac aaactgactt gtttcttttt gctcaaagta
     8941 agtcgctgtt aaattatcct cgccgtcaat gtatttttgt tgaataagct gatgtacaat
     9001 ctttttagct acttccttgg tgacagtcag tttttgacca gcttcatttt ccaagacctt
     9061 atcgacaaag aggtccactt ccaccttact tggtcgatca gccaagattt ccctgagttc
     9121 tctttgcaag ttcttagcaa aatcctcgta gctttcattg gcaataacgg tcaatttatt
     9181 aatgttatga acatctgctc ctaagagttc ctcatcctga cggacacctt tttggtcaac
     9241 agcaagacgc atcccacgac caatcttttg ccgtttttct gtctccgtac ccgtattttt
     9301 cagagtacaa atctggaaga cattaggatt gtcccagcct tctttgaggg ctgagtggga
     9361 gaagataaag cgtactggct catcaaagga cagcagacgc tccttgtcct tcataatcaa
     9421 atcaaaggca tccttgtcgt tagactgatt attcctcttc tcagttgccg atttgtaatc
     9481 cacaaacttg accttgtccg attttttcac cttatcaacg gagaaataac ctgcgtgaac
     9541 gggattgcct ttctgatgat tatttagata atgccaaaaa ttgctgccat ggaggttggg
     9601 atcagccaac aaggccttga cctgggcttc gtattcttct tcaaatatct gagcataggt
     9661 tccattgtag gcatcatttt gctcgtcgta gcacttgtac ttggcaactt catcaatgaa
     9721 gaaaagggat agcaccttga taccacgttt atataggcct tcttcttttt gcaggtgact
     9781 ggcaatggtt tcccgaatct ggatacgacg gatatcatct tcgttggttt ccccaatcac
     9841 ttcgcccaca tggagtttgc gattggcacc gatttgaatg gaattttcgc cctcacgcgc
     9901 atcaaaatga ctcaaggtcc aaccatcttg atatgatgga agattccccg actcttcata
     9961 aagggcaaac ggttcttcaa ccagcttggt cactcgtttg acatcgcttg acttggttcg
    10021 catctcaaac tcaatccgag cctttggagc tccagatttt tgcggaacta gttcttgcag
    10081 ataaaggtag ccttgtgtac ctgtggtaga agtctgctcc acggtcttaa cggcaatctt
    10141 tttaaccaac tttttattat aggcgtccaa ggcatccaga cgataaatca tatcgtgctt
    10201 gtccttatgg gtggctgaat agcggagggt aaataggggc ttgaaatcct ccaagcgttc
    10261 tttagtcttt ttcccctcaa ccgactgagg ctcatcaatg attaaaatcg gattggttgc
    10321 tgcgataaca tcgatgggct ttcgccaacg aaaactttcc tgctcggtgt gaatccgacg
    10381 ggcatccgct ccagttgcat taaaggcctg ggagttgata atcatgacct gaatgtccga
    10441 ggaattggcg aacttgtcta gctctcccaa acggctagaa tcatagacaa agtagcgtgg
    10501 cttcttgccg tactccatct ggaaatggtc tgccatgatc tcaaaggact tgagcacccc
    10561 ctcacgaatg gcgatgctgg gtaccacgat gataaacttg agccagccat actgcttatt
    10621 gagctccatc agggtgcgga tgtaggtata ggtcttccct gtccctgttt ccatctcaat
    10681 ggtcaaattg taagacggac gcccatccac tttttggatg gataggtttt cggaaggttt
    10741 aagtccatgg cggagctgct gagttcgtac ctgctggctg atagtatctt gtgaaagctg
    10801 gatgggagca ttgcggaaac cgataaaatt catgtccagt tccgcaccca ttccctgacc
    10861 tttatccatg tggtaatcaa aaccgctatg tttgccctga ccagcaaaaa catccacaac
    10921 agaggccacc gcatctaact gaaattgctg ctgcttaaat tgaagtttca ttggttgcct
    10981 ccttctttgt gaaatctatc cttcaatcgt aatactttag tcttagtcaa aactctaaat
    11041 gcttgactat caattttaag aacttctgca agtgtctttg cctcatacaa aactctgctt
    11101 gtcccaacag atttattatt tccatattct tcaaaacttt tgataaaatc cagtaattgt
    11161 tggtagacaa ttttatttgt aaagaaatat gatttagcaa tcaatttgtt caactccgtg
    11221 tagagctttg ttgtctcctc aacagatagc tttctaatat tcaacacatc atcaagtaaa
    11281 tacatgagct cagaataaat tgttattttt tcttcataga actttgtctt gttccctaat
    11341 aagtaaccaa gtaatgctgt aataagtcca aaaggtattg ataacccttc aaaaacactt
    11401 cctaactcca tcaccctaca ccaccttcac tttggtattt ggcgcttctt ctttgaagat
    11461 ttgaccgagg ttgattttgg ctgcggagtt ggtgaaggat tcatccttaa aaacagcacg
    11521 gagtggctct tctttggcaa tttgacggat gacgctttcc gaaatctcat ctgcaaagca
    11581 ggcaatcaag gcaccatctg ccacattgta aacagctgta ccgtcaattt gagttttcgc
    11641 aatcggaaga gataattcca tgccccagat cagcataatc tggaagagca agtcaaggtc
    11701 tgtccgaccg tctttgatgt ttgaaacact atcaaaaaga ctttcttggc taaagttgtc
    11761 tggtgtttgg ctgacatcct tgaagtttga actatctaac ttataagctc taaaaccaaa
    11821 gtcttgcttg ccgacggttt caggatgttc ttcctgaatc tttttagctg cccgacggat
    11881 ccgctcacga gaaatctggt caatggtttc ataaccagct tctttggcag ctgccttgtc
    11941 tgccacctgc tcgtccagcg tacaaagaat atacttacga ttacctccgt cttcagcgtt
    12001 caactgcata accgcatcag ccgtggttgc cgaaccagcg aagaagtcta aaatgttgat
    12061 atccctttcc gaactagtaa actttatcaa ctcctgaatt actttgggat ttttaggact
    12121 gtcaaaagga atctctaatc ctgttggttt tgaaatcagc atatcagacc aatttgagtt
    12181 tcttagaata ccatccgatg gtgctaccca atgttctaca cctttatttt ttccgtttcc
    12241 atttagattt cttctcaaaa atttcttagt agaaccagta tgtttccagt actcttcaat
    12301 actcatcgtt tctgaaaaat tttcaagata tacttgatag ttatgaatcg cttcttcagc
    12361 aagctctttc ttccacttcc attgaccttc atcgggcgta tagcctaaaa tttcatatct
    12421 cattgtaggt ctatcagcat tattccaaaa tcctttccag tacccgcttt gagaacgtac
    12481 ttctgaagat tctttaaaaa ctgggttaaa cctacttatt tgactcttcg aatagacaaa
    12541 aatgtattct aacccaacat tcatagaatg aagtccttca gccataaatt gtctattcaa
    12601 atttttatca taccttctcg tagcaattga attcctaaaa ttctcctccc caaatatctc
    12661 atcacagata gctttgaggt tggattgttc attgtcgtca atggagataa agataacgcc
    12721 actgtctttg aggaggtttc tggcaaggac taggcgaggg tacatcatat tgagccagtc
    12781 ggagtgatag cgggcggagg atttttcatt cttggtcaag cccacccgtt gcaaaccatt
    12841 ctcatctcga aggtccattt catctgccag ttcttcctcg ctcatctgga atttatctga
    12901 gtagacaaag tccttgcctg tattgtatgg cggatcgatg taaatcatat caatcttgcc
    12961 cagataagac tcctgcaaga ccttgaggac ttccaggttg tcccctgtaa taaagacatt
    13021 ctcagacttg tcaaagtcaa cactttcctc aagatctggg cgaagggtct tgaggctggg
    13081 tttaccagcc tcggcaatcg ctccgcgttt gccgacccag gtaaactcat aggactcccg
    13141 cccctcaaca atctctcttg aaaggaattg tcgtagcttg tcaaagtcaa tggctggtcg
    13201 taacctgcca ttttcatcac tactttctgt tagaacctca ggaaaaagct gaccaatctt
    13261 tgcaatgtta tctgataaaa tatcttttga cgttaggtct agctgttcaa cttgagtgta
    13321 ttctccaagc tt
//
LOCUS       DATUCG010000063        13291 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.064, whole genome shotgun sequence.
ACCESSION   DATUCG010000063 DATUCG010000000
VERSION     DATUCG010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13291
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.064"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(19..3672)
                     /gene="addA"
                     /locus_tag="VCA20_001427"
     CDS             complement(19..3672)
                     /gene="addA"
                     /locus_tag="VCA20_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEP1835237.1"
                     /translation="MAFEEFLSAEEIRAVQLAEANSDKQQKRTPEQIEAIYTHGQNVL
                     VSASAGSGKTFVMVQRILDKLKRGVGIDQLFISTFTVKAAGELKERIEKNLSETIAET
                     TDMELRRHLSAQLADLTKADIGTMDSFTQKLVTTYGYSLGISPQFRILQDETEKSSLK
                     KEVFDQLFADYLEQDGNGAFRRLVRNFSGNRKDNGGFRQVVYQVHDFSQSTSSPTKWL
                     KEQAVQADLYSQERIELILEQGFKEKVLDKLYQAADFFRYHVEWGRNDFGSAKYFANV
                     EEVLDLLTGLDSLDRKDLMERVEKILLINDQSRGKGLTNANRPKDEHLIAFKEEYNAG
                     KSQLISGLRDLGQEVYELTLLKDYQIQVLPLLILLRDFVLDFSQAYLDVKIKEAAFEF
                     GDIGYFAIRILEENVDIRQFFQEKYHEVMVDEYQDNNHSQERMLDLLSNGHNRFMVGD
                     IKQSIYRFRQADPMIFQEKFELYQANPQAGKLILLKENFRSQIEVLEATNAIFTRLMD
                     RQVGEIKYDDTHSLVAGSPGQKIAQPKHEMEYLIYDQQDSANSSTDAEEETPLTAGEI
                     EVVAKEIIRLHNEEGADFKDITLLVQKRTHNDLIMSIFEKHGIPIVADGGAASYLQSL
                     EVMIMLNTLRVINNPLNDYALVALLKSPMFRFDEDELTRISLQAGTGFFYQKMEIAQQ
                     TNGQHPELMSGELKKKIKDFLSILENWRTYAKLHSIYDLIWKIFNEKFYYDYVGALPN
                     GSKRQANLYALGLRANQFEKTGYKGLSRFIAMIDRALANDKDLADVQEFLPQNAVQLM
                     TIHKSKGLEFKYVFLMNIDKRFNLEDHYQSVIISRKNGLGIQYLADMKDKVNSPLPQV
                     RVLMNTLPYQNNLQELKIANLSEQMRLLYVALTRAEKKLYLVGKGNADKLAEKYDGKK
                     ENGVLAQSTRESMATFQDWILAIDEAFSGEDLHFKKVFVTDEDLTEEKIGKLTLKSKL
                     EDTSLKDIRQSEDIAQALDQLSSVQELNERYKAAIELPSLRTPSQIKKLYEPILEQEG
                     MEVMEKYQPKRTFNLPDFSKKPKITGAQVGSAVHELMQRLDLSWLVTEDTVREALEAV
                     HAELVVKDKINVQMILDFFDTDLGREILANTDKLHREAPFASLQTDPVSQENFVLRGI
                     IDGYLLYEDHIVLFDYKTDKYDQPIQLSQRYQAQMQLYAEALKKAYKIDRVDCHLILL
                     GGERIEVVEVKVN"
     gene            complement(3672..6938)
                     /gene="rexB"
                     /locus_tag="VCA20_001428"
     CDS             complement(3672..6938)
                     /gene="rexB"
                     /locus_tag="VCA20_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HEP1835238.1"
                     /translation="MKLVYTDIRNPLTQYLTDTAAEFVKEGKRVFYIAPNSLSFEMER
                     KVLEYLPEQATFDIIVTRFGQLARYLMIDRKEAGQPLDDVGLAMIFFRVLSQFEDGDL
                     KVYGRLQTDFGFINQLVALYKELQRANMSILDLEAMDSPDKQADLVKIFLAVTDILSK
                     EGFEHQSKLAQLTGLVETGQLDEQLKNIVLVVDGFSRFSAEEEALVSALNERVSEILI
                     GVYASKKAVQVTYAEGNVYQANVDFLRQLSAQFQTKATYIGQEPVLDSIGKFSKNMEA
                     YYDYSGTMIDLTPADQEKIQLWEVVNQKEEVEQVATAIRQHVHQGARYKDILLLLGDL
                     DSYKLQIGKIFDKYDIPYYFGKAEEMSHHPLVHFVESLERLRRYRFRAEDLLNLLKSG
                     LYASISQKELDLFESYILFADMKGQAAFSRAFVTNSRAEYDAEVIKEKRLVYDLTVLE
                     PLRAKIMEPLNQLFKAGPQSGTALLEKFMAFLEAIELPKNMEKMSRNLSEVEQEKEEQ
                     VWKSFTHLIENFHQIFGKEKLKMDDFLAILQAGMQASHYRTVPATVDVVNVKSYDLIE
                     PHTAKYVYAIGMGQSNFPKVAKNTSLLTEEEMEKVNLISASSLRFDLVSRENIKKNHA
                     AMMSLLNSATEQLVISTPQIYNEGEDSLSPYIKILQKMGLKSEERGRIKTLSPQDIGH
                     YKSLLSRLIESERPSLDNEEWEGQTAFWTVLVRHLKKKLESQGIEIPTITGEITSKQL
                     AADTLAALYPEDKPLNLSASSLTNFYNNQYLYFVRNVLRLREQESIHPTAFQHGLFLH
                     RIFERVVMDQSELDFDQKVDKAILRTRDEAEFAMFYNQDADARYTEEVLDKIARSSAT
                     ILRDNDLVEIDGQEKSFRQDKALVFDLQNGKSVHVNGTIDRLDTLQINQAVGVVDYKS
                     SDQSFSIGDFYNGLKPQLVTYLTALQELDETKDKPVFGAMYLHLQDPIIKLKDTKNLE
                     QLEGAANTSLGYKGLFLKEESLGLNHFYQTRNQLYTEDEFAVLLNHNRELYKQAAKDI
                     LDGRFAINPYTKDGRSVAGEQLKAITGFEADRHMGMARRLVKEAKRQDWMERMKGGQD
                     "
     gene            complement(7102..13191)
                     /locus_tag="VCA20_001429"
     CDS             complement(7102..13191)
                     /locus_tag="VCA20_001429"
                     /inference="COORDINATES: protein
                     motif:HMM:NF018196.1,HMM:TIGR01168.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEP1835239.1"
                     /translation="MHHSRRKSFDWYGLKQHFSIRKYHFGAASVLLGMSIALGTGTQV
                     VQAAETVTTPESTSVASSTSATESTTSETSTETATSSSSERTVEIGYIVKYATEDGTL
                     VNASVETVSVTTTETIAKATVSVTAVVPESYELAAGQSATVSQEVTENGANIVTVKVV
                     KKAEEKAATSETTTTASTETATAKSTEDTATTPATVSEAVKTPATVEEAKKVLEQVTS
                     EAEVLANEGNRIVATDTTTDTSALKAVAAATKLAATDATSVLQDTVATLDALNAQITA
                     VRTNVEALALELRKYLGTDVIEIALATVTDLNDATNRPGYWTENEEVLNKEAATATAT
                     IKPQEQRIPAGYAVDPTDGRITFLIYSLSADDVDAGKSAGIQDGSYNLRYGTDYYITL
                     SMDRTASDGVIYARLVSKQKGFIEEVAIPENTSRHQFQTIANGPTNLGRFTYRMSLTT
                     MPFTDSTGQQTSARYVNINSGLGGNAKGSVAYSLLTSDATSYNSYTGLAPSILPTQIT
                     SYLVKETERRSEELLASYAHLGEVSGDSFTISDAAEFANYELIESPEIKDTTISTNYS
                     VGTPLIQYFPGAKTARVIYITKEDGTARFVNFMLNPDHPDFLENFKKYHPMSPVDFVT
                     NGGAEALDFSSRIAIIDKYIAEIDEVKADTSLSRDQQASRLAELRANYATEINTDDNK
                     FLMTFVSTEAEPLTANDGPDAVTGLSTWKHTLKNYYVNGNRDTTIALQNTAYLNDPMV
                     DWSPTAPTQFGVQLHSKTELNDKGTPLRLSIPIDDRGTTVFGDVHTNIIAMFNMLTEN
                     ENNQHYYYAEKGGVKVYYVDTEGKVLQDAKSVYDHEATNTAYDTKSVKDETITTADGT
                     VYYYKEIDTTGLNPASSNTDTEKRTIEKITEEVGTVAQDTLKEMTYVYEKAGSVIVNY
                     ITEDGTPLSGKTNTGATTASTVDDTKDGKPGSKYDTNDLKPTTITTDDGKTYELVPAS
                     TVGDETGTVEAGVTKKVTYIYKEVKGDVIVHYVDTEGNPISGTTDEGKQTASTVTDTP
                     ESSTGTRYDTKDLKPKTITTNDGKIYKLIPAATQGNETGEVVVGTTEITYVYELVQGD
                     VIVHYVDTEGNTIADDVTDQTITDTGTDYDTRDNKPEKIVNDETGDVYYILPKDEVKA
                     GDKETGKVVEGTTEVTYIYQKAGSVNVNYVDTEGNVLKDPVADVTDGKPGSGYDTVLD
                     NKLASISVDGKLYRLAPANTYNVGTVSGDNNLTAVGNGKATGIDATTGTVEAGVTKEV
                     TYVYQEVKGDVVVEYYDTEGNQIAKTVVDEDDKSVGTVYNTDEDNKPEKITTEDGTVY
                     YYKEVKDTSAPTTGKVAETTTTVQYVYEKAGSVNVNYVDTEGNPIQAPVADETNEKAG
                     TEYATADNKPTTITTADGKTYRLVEAGTYNVGTVSDDNNLTAVGNGKATGIDATTGTV
                     EAGTTKEITYVYEEVKGNVVVNYITTDGTVIKQPVEDTPTSSTGTPYDITDNKPTTIT
                     TEDGKTYRIVPVLTKGSETGKVVEGTTSITYVYEEVKGDVVVEYYDTEGNLISGLSDS
                     GEAVDTKEVDTPSTSTGTAYNTDEDHKPNTITTADGTVYYYKEVKDTSASTTGKVVEG
                     TTTVQYVYEKAGNVIVHYITEDGTVIKATVTDEENAEPGKSYDTTDNKPTEIVTEDGS
                     RYVLIPSKTIGTENGTVEGGKTIEITYVYKKVANWIPQIPGVPEGQEPKVPYPFDPTN
                     PDVPVTPTPDTVIPNVPGYTPVDPKTNEPLKPVAPTDPGKGYVPPTPDDSSVDTPVPY
                     VQNGNVVVNYVTEDGTVIKTPVNDETDAPAGKSYDTTDNKPNTITTEDGTTYELVRVE
                     GSETGTVVGGKTTEVTYVYRKVETPAKKVVTNHVDEDGNPIAPQEEGTTPNKSIPGYE
                     FTGKTVTDPDGNTTHIYRKVTKVVTNHVDEDGNPIAPQEEGTTPNKSIPGYEFTGKTV
                     TDPDGNTTHIYRKVSKPVAPKPQAPVAPTTAKAGAAQLPNTGEASSSAGVLGAAMLVA
                     AIALAGKRRRNED"
BASE COUNT         3458 a         2973 c         2597 g         4263 t
ORIGIN      
        1 gagtttaacc actcgctttt agtttacctt gacttcaacc acttctattc tttccccacc
       61 aagcaaaatc aaatggcagt ctacacgatc tatcttgtag gcttttttca atgcctcagc
      121 atagagttgc atttgagctt ggtaacgttg gctgagctgg attggttggt catatttgtc
      181 tgtcttgtaa tcaaagagga caatatgatc ctcatacagt aggtagccat cgataatccc
      241 acgaaggaca aagttttcct gagatacagg atcagtctgc aaacttgcaa atggagcttc
      301 tcgatgaagt ttatccgtat tggctagaat ttctcttcct aagtctgtat caaagaaatc
      361 caaaatcatc tggacattga ttttgtcttt aaccactagc tcagcatgaa cagcttctag
      421 ggcttctctt accgtatctt ctgtcaccaa ccaagacaga tccaagcgtt gcatgagttc
      481 atggactgct gaaccaactt gggctccagt gatttttggt ttcttggaga aatctggcaa
      541 gttgaaagtc cgttttggct ggtatttttc cataacttcc atgccttctt gttccaaaat
      601 aggctcatag agtttcttga tttgacttgg agtgcgtaaa cttggcaatt ctatagcagc
      661 cttatagcgt tcgttcaact cttggacaga agacaactgg tccaaagcct gggcgatgtc
      721 ttctgattga cgaatatctt taaggctggt gtcttctagc ttacttttca atgtaagttt
      781 accaatcttc tcctctgtca aatcctcatc cgtcacaaaa actttcttga agtgcaagtc
      841 ttctcctgag aaggcctcat caatggctag aatccagtct tggaaggtcg ccatgctttc
      901 acgagttgat tgagcaagta cgccattttc ctttttacca tcgtattttt cagctaactt
      961 gtcagcattg ccctttccaa ccagatacag cttcttctcg gcacgcgtta gtgcaacata
     1021 aagcaaccgc atttgttctg aaagattggc aatttttagc tcttggagat tattttgata
     1081 aggaagtgtg ttcattaaga ctcgcacctg aggtaatggg ctatttacct tatctttcat
     1141 atccgctaaa tattggatac ctagaccatt tttccggctg ataatgactg actggtaatg
     1201 gtcttccaag ttgaatcgct tatcgatgtt catgaggaag acatatttga actccagacc
     1261 ttttgactta tgaatggtca taagctgtac agcattttgc ggtagaaact cttgtacatc
     1321 tgccaagtcc ttgtcatttg ctaaggcccg gtcaatcatg gcgatgaagc gggacaaacc
     1381 cttgtagcct gttttttcaa actggttggc acgcagacct agtgcataga gattggcttg
     1441 acgcttgctg ccatttggaa gagcaccgac atagtcatag tagaattttt cattgaaaat
     1501 cttccaaatc aagtcataga tagaatgcag tttggcatag gtacgccagt tttccaaaat
     1561 agacaagaaa tccttgattt tcttcttcaa ctctcctgac ataagttcag gatgttgccc
     1621 atttgtttgc tgggctattt ccattttctg ataaaagaaa cctgtcccag cctgcaagga
     1681 aatccgtgtc agttcatctt catcgaatcg gaacataggt gatttaagca gggcaaccaa
     1741 ggcatagtca ttgagtggat tattgataac ccgcaaggta ttcagcataa tcatgacttc
     1801 taaagattgc aaataggagg ctgctccgcc atctgccaca attggaatcc catgtttttc
     1861 aaaaatggac ataatgaggt cattgtgcgt ccgcttttgt accaagagag tgatgtcctt
     1921 gaagtctgct ccttcttcat tgtgaagacg aataatctct tttgcaacga cctcaatttc
     1981 accagcagtc agaggtgttt cttcctcagc atccgttgaa gagtttgcgc tatcctgctg
     2041 atcgtaaatc aagtattcca tctcatgctt gggctgagca attttttgac caggactacc
     2101 agccactaga ctgtgggtgt cgtcatactt gatttcccct acttgacggt ccatcaggcg
     2161 cgtgaaaata gcatttgttg cctccaagac ctcaatctga ctacggaaat tttccttgag
     2221 caaaatcaat ttcccagcct gaggatttgc ctgatacagt tcaaatttct cttggaaaat
     2281 catcggatcc gcctgacgga atcgatagat ggactgctta atgtcgccca ccataaagcg
     2341 gttgtgaccg ttagacagca ggtccagcat tctctcttgg ctatggttgt tatcctggta
     2401 ctcgtccacc atgacttcgt ggtatttttc ttggaaaaac tggcgaatgt ccacattttc
     2461 ttctaaaata cgaatagcga aataccctat atcgccaaat tcaaaagctg cttccttgat
     2521 tttgacatcc agataagcct gcgaaaaatc caagacaaag tcacgtaata atatcagcag
     2581 tggcaaaact tgaatctgat agtctttcag cagggttaat tcataaactt cctgacccaa
     2641 atctctaagt cctgagataa gctggctctt gccagcattg tattcttctt taaaggctat
     2701 caaatgctca tcttttggtc gattggcatt ggtcaggccc ttgcctctgg actggtcatt
     2761 gatgagaagg attttttcaa cccgctccat caagtccttc cggtccagac tatctaaacc
     2821 tgtcagcaaa tccaagactt cttccacatt tgcaaaatac ttggcagatc caaaatcatt
     2881 tctgccccat tccacatggt agcggaagaa atctgcagct tgatagagct tgtctaacac
     2941 tttttcctta aagccttgct ctaagatcag ctctatacgc tcttgactgt acaaatcagc
     3001 ttgaacagcc tgctctttca gccatttagt tggactgctc gtcgattgac taaaatcatg
     3061 cacctgataa acaacctgac ggaaaccgcc attatccttg cggttgcccg aaaaattgcg
     3121 aaccaatcta cggaatgcac catttccatc ctgttcaaga taatctgcaa agagctggtc
     3181 aaaaacctct tttttcagac tcgatttttc tgtctcatcc tgtaaaatcc gaaactgtgg
     3241 agaaatgcca aggctataac cgtaggttgt caccaatttc tgcgtaaagg agtccatggt
     3301 cccgatgtct gccttggtca aatccgccaa ctgggcagac aaatgccgac gcaattccat
     3361 atcggtcgtc tccgcaatgg tttcactgag atttttctca atccgctcct tcaactcacc
     3421 agccgccttg actgtaaagg tcgagataaa gagctggtca atcccgacgc cacgcttcaa
     3481 cttatccaaa atccgttgaa ccataacgaa ggtcttgcct gacccagccg atgcagagac
     3541 cagaacattc tgaccgtgcg tgtagatggc ttcaatctgc tcaggtgtgc gtttttgttg
     3601 cttatcagaa ttggcttccg ccagctgcac agccctgatt tcttccgcgc ttaaaaattc
     3661 ttcaaaagcc attagtcctg acctcctttc attcgttcca tccaatcttg acgcttggcc
     3721 tctttgacca accgccgcgc catgcccatg tgccgatccg cctcaaaacc agtgattgcc
     3781 ttcagctgct cacctgccac cgaacgccca tctttggtat aaggattaat agcaaagcga
     3841 ccatccagga tgtccttggc agcttgtttg tagagttctc ggttgtggtt caataaaacc
     3901 gcaaactcat cttccgtgta gagttggtta cgagtctgat aaaaatgatt gagccccaga
     3961 ctttcctcct tcaagaaaag ccccttgtaa cccaagctag tattggcagc tccttctagc
     4021 tgttctaaat tcttcgtatc ctttaatttg ataattggat cctgcaaatg caaatacata
     4081 gccccaaaaa caggtttatc cttggtttca tccagctctt gtaaggctgt caagtaggtc
     4141 accaactgag gtttgagacc attgtaaaaa tccccaatcg aaaagctttg gtcactggac
     4201 ttgtaatcta caacaccgac agcctgattg atttgtaagg tatccaagcg gtcaatggta
     4261 ccatttacat gcacactctt gccattctga aggtcaaaaa ccagcgcctt atcctggcgg
     4321 aaactctttt cctgaccatc aatttccacc aaatcattat cccgcaaaat agtcgcactg
     4381 gagcgagcaa tcttatccag cacctcctcc gtataacgag catccgcatc ctggttataa
     4441 aacatagcaa attctgcctc gtcacgcgta cgcagaatgg ctttgtccac tttttgatca
     4501 aaatctagct cagattggtc catgaccact cgttcaaaaa tccgatgcaa gaacaaacca
     4561 tgctgaaagg cagtcggatg gatggattcc tgttcccgca ggcgaagaac attacggaca
     4621 aagtaaagat attggttatt gtagaaattg gtcaagctgg atgctgaaag attgagcggc
     4681 ttatcctctg gatataaagc agccaatgta tcagctgcaa gctgtttaga agtaatttcg
     4741 ccagtaatgg tcggaatttc aatgccttgc gactccaatt tcttcttcaa atggcgaacc
     4801 aagactgtcc agaaggccgt ctgcccttcc cattcttcat tatcaagaga tggtctttcc
     4861 gactcaatca aacgggataa gagactcttg tagtgaccga tgtcttgcgg actaagtgtt
     4921 ttaatccgac cacgttcttc ggatttcaac cccatttttt ggagaatctt gatataaggg
     4981 gagagactat cctcaccttc gttataaatc tgaggagtag aaatgactaa ctgttccgtc
     5041 gcagaattaa gcagagacat catagcagca tggttcttct taatattctc tcggctgacc
     5101 aaatcaaaac gtaaagaact agccgaaata agattgactt tttccatctc ttcttctgtc
     5161 aatagactgg tattcttggc aacttttgga aaattggact ggcccatccc gatagcataa
     5221 acatactttg cagtgtgcgg ctcgatcaaa tcataggatt tgacattgac cacatcaacc
     5281 gttgcaggaa cggtacgata gtgactggcc tgcataccag cctgtaaaat agccagaaaa
     5341 tcatccattt tcaacttttc ttttccgaaa atctgatgaa aattctcaat aagatgggta
     5401 aaacttttcc agacctgctc ttctttttct tgctctactt cgctcaaatt tcgagacatt
     5461 ttttccatat ttttaggaag ctcaattgcc tccaaaaatg ccataaattt ctcaagcaag
     5521 gcagttccag attgtggtcc agccttaaac agctggttca aaggttccat aatcttagca
     5581 cgcaggggtt ccaagacagt caaatcatag acaagtcgct tctccttgat gacttcagcg
     5641 tcatattcag ctctgctatt ggtcacaaaa gccctcgaaa aagcagcctg ccccttcata
     5701 tccgcaaaga ggatgtaaga ctcaaatagg tccagctcct tttgggaaat gctggcgtac
     5761 aaaccagatt tgaggagatt gagcaggtct tccgctcgga aacgatagcg acgcaagcgc
     5821 tccaaggact ccacaaaatg gaccagagga tgatgactca tctcctctgc cttaccaaaa
     5881 tagtaaggaa tatcatactt gtcaaaaatc ttgccaatct gcaacttgta gctatccaaa
     5941 tcccccaaga gcaacaagat gtctttatag cgagctcctt gatgcacatg ctgacgaatt
     6001 gcagtcgcaa cttgctccac ctcttctttt tggttaacaa cttcccatag ctggattttt
     6061 tcttggtctg ctggagtcaa gtctatcatg gtcccgctgt agtcataata ggcttccata
     6121 ttctttgaaa atttaccgat actgtccaag actggctctt gaccaatata agtagcctta
     6181 gtctggaact gcgctgatag ctgacgtaaa aagtcaacgt tggcttgata gacattgcct
     6241 tccgcatagg tgacttgcac agctttcttg ctggcatata caccaattag gatttccgac
     6301 acccgttcat ttaaagcact tactaaagct tcttcttctg ccgaaaaacg agaaaatccg
     6361 tctaccacca gcacaatgtt cttcaactgc tcatccaact gaccagtttc taccaagcca
     6421 gtcaactgtg ctaacttaga ctgatgctca aagccttctt tggacaaaat gtctgttaca
     6481 gccagaaaaa tttttaccaa atcagcctgc ttatctggtg agtccatggc ttccaagtcc
     6541 aaaatagaca tatttgcccg ttgcaattct ttgtataaag caactagctg gttaatgaaa
     6601 ccaaaatctg tctgcaaacg accatagact ttcaagtcgc catcttcaaa ttgcgataga
     6661 acacgaaaga aaatcatggc cagaccaaca tcatccagcg gttgacctgc ttctttccta
     6721 tcaatcatca aatagcgggc caattgccca aaacgggtca cgataatatc aaaagttgcc
     6781 tgttcaggca ggtattccaa gaccttacgt tccatctcaa aggatagaga gtttggagca
     6841 atataaaaaa ctcgtttacc ttccttaaca aattctgctg cggtatccgt taaatactgc
     6901 gtcagaggat tacgaatatc tgtatagact aatttcatat caaattctca tgctttcatt
     6961 gaatatacta tgtctagtat atcaaaatct aacaaaaaaa tggtccaatg gaccattttt
     7021 tcagaaaggt aattctttct acatttatct aaatttcttc acttattcta tcgggggaag
     7081 aattcgtgta aagaaatata attagtcttc gttacgacga cgtttgcctg caagagcgat
     7141 tgcggcaact agcatcgctg caccaaggac gccagctgaa gatgaagctt cgccagtatt
     7201 tggcaattga gccgcaccag ctttagcagt tgttggagct acaggagcct gtggttttgg
     7261 tgcaacaggt ttgcttacct tacggtagat gtgtgttgtg ttaccgtctg gatctgttac
     7321 agtcttacct gtaaactcgt atcctgggat tgacttgttc ggtgttgtac cttcctcttg
     7381 cggtgcaatt gggttaccgt cttcatcaac gtggtttgtt acaaccttag ttaccttacg
     7441 gtagatgtgt gttgtattac cgtctggatc tgttacagtc ttacctgtga actcgtatcc
     7501 tgggattgac ttgttcggtg ttgtaccctc ttcttgcggt gcaattgggt taccatcttc
     7561 gtcaacgtgg tttgttacaa ctttcttagc aggtgtttct actttacggt aaacgtatgt
     7621 tacttcagta gtcttaccac ctacaacagt accagtttct gaaccttcca cacgaacaag
     7681 ctcataagtt gtaccatctt ctgtagtgat agtgtttggc ttgttatcag ttgtgtcata
     7741 agacttacct gctggagcgt ctgtttcgtc gttaacaggt gtcttaatta ctgtaccatc
     7801 ttcggtcacg tagttcacca caacgttacc attttgaacg taagggactg gtgtgtcaac
     7861 gctagaatca tctggtgttg gtggcacata acccttacct ggatctgttg gagcaactgg
     7921 tttcaatggt tcgttggtct taggatctac tggtgtgtag cctggaacgt ttggaatcac
     7981 tgtatctgga gttggtgtta ctggtacatc tgggttagtt gggtcgaatg gatatggaac
     8041 ttttggttct tgtccttcag gtacacctgg aatttgtggg atccagttag cgactttctt
     8101 atacacataa gtgatctcaa ttgtcttacc accttcaact gtaccgtttt ctgtaccaat
     8161 tgtcttagac ggaatcaaga cataacgtga accatcttct gtaacaatct ctgttggttt
     8221 gttatctgtt gtgtcataag acttacctgg ttcagcgttt tcttcgtctg ttacagtagc
     8281 cttgatcact gttccatctt ctgtgatgta gtgaacgata acatttccag ctttttcgta
     8341 aacatattga acagttgttg taccttctac aactttacct gttgttgaag ctgaagtgtc
     8401 tttcacttct ttgtagtagt aaactgtacc gtctgctgtt gtgatagtgt ttggtttgtg
     8461 gtcttcatca gtgttgtatg ctgtacctgt tgatgttgat ggggtatcaa cttctttagt
     8521 gtctacagct tcacctgagt cagatagacc tgaaatcaag ttaccttctg tatcgtagta
     8581 ctcaactaca acgtcacctt tcacttcttc gtatacgtaa gtgattgatg ttgtaccttc
     8641 tacaacctta cctgtttctg aaccttttgt aagaactggt acgatacggt atgtcttacc
     8701 gtcttctgtt gtgattgttg ttggtttgtt gtctgttatg tcgtacggag tacctgttga
     8761 tgatgttggt gtgtcttcaa ctggttgctt gatcactgta ccgtcagttg tgatgtagtt
     8821 aacaacaacg ttacctttca cttcttcgta aacgtaagtg atttcttttg ttgtaccagc
     8881 ttcaactgta ccagtcgtag catcgatacc tgttgcttta ccgttaccaa ctgctgtcaa
     8941 gttgttatca tctgaaactg taccaacgtt gtaagtacct gcttcaacta gacgataagt
     9001 cttaccatct gctgttgtga tagttgttgg cttgttatca gctgtagcgt actcagtacc
     9061 agctttctcg tttgtttcgt cagctactgg tgcttgaatt gggttacctt ctgtatcaac
     9121 atagttaaca ttgacagagc ctgctttttc gtagacatac tgaacagttg ttgttgtttc
     9181 tgctacttta ccagttgttg gtgcagaagt atctttcact tctttgtagt agtaaacggt
     9241 gccatcttca gtagtgattt tctctggttt gttgtcttcg tcagtgttgt agacagtacc
     9301 aacagactta tcgtcttcgt ctacgactgt tttggcaatt tggttaccct ctgtatcgta
     9361 gtactcaact acaacgtcac ctttcacttc ttggtaaacg taagtaactt ctttagtcac
     9421 ccctgcttca actgtaccag tcgtagcatc gatacctgtt gctttaccgt taccaactgc
     9481 tgtcaagttg ttgtcacctg aaactgtacc aacgttgtaa gtatttgccg gagcaaggcg
     9541 gtaaagtttg ccatctactg agatgctagc cagtttgtta tcaagaacag tgtcatagcc
     9601 agaacctggt ttaccatccg tcacatccgc aactggatct ttaagaacat taccttctgt
     9661 atcgacatag ttaacattaa cgctaccagc tttttggtag atgtatgtaa cttcagtcgt
     9721 accttctaca actttaccag tctctttatc gcctgctttg acttcatctt ttggcaagat
     9781 gtagtaaaca tcgccagttt cgtcattaac gattttctct ggtttgttat ctcttgtgtc
     9841 gtagtctgta cctgtatctg tgatagtttg gtctgtcaca tcatctgcaa tggtgttacc
     9901 ttctgtgtcc acatagtgaa cgataacatc accttgtact aattcgtaaa cataagtaat
     9961 ctctgttgtg ccgacaacaa cttccccagt ttcattacct tgtgtcgcag ctggaataag
    10021 tttataaatc ttaccatcat ttgttgtgat agtttttggt ttcaggtctt ttgtatcgta
    10081 tcgagtacct gttgagcttt ctggtgtatc ggttacagta cttgcagttt gcttaccttc
    10141 gtctgttgta cctgagattg gattaccttc agtatcaacg tagtgaacga taacatcacc
    10201 tttaacttct ttatagatat aagttacttt tttagttaca cctgcttcta cagtacccgt
    10261 ttcatcacca actgtagacg ctggaaccaa ctcataagtt ttaccatcat cagtagtgat
    10321 tgttgttggt ttcaagtcat ttgtatcgta tttagaacct ggtttaccat ctttggtatc
    10381 atcaactgtg cttgcagttg tagctccagt atttgtttta cctgaaagtg gagtaccatc
    10441 ttcagtgatg tagtttacaa ttacactacc agctttttcg tagacataag tcatttcttt
    10501 aagcgtatct tgtgctactg taccaacttc ttcggtaatt ttttcaatcg tacgtttttc
    10561 agtgtcggtg ttgcttgaag ctgggttaag acccgttgta tcgatttctt tgtaatagta
    10621 aactgtaccg tcagctgttg tgatagtttc atcttttaca gacttcgtat catatgctgt
    10681 gtttgtagct tcatgatcat aaacagactt agcgtcttgt aaaaccttac cttctgtatc
    10741 aacgtaatag actttaacgc cacctttttc tgcatagtag taatgctgat tgttctcatt
    10801 ttcagttaac atattaaaca ttgcaataat gtttgtatga acatcaccga atactgtagt
    10861 cccacggtca tcgataggaa tacttaaacg taaaggagtg cctttatcgt tcaactcagt
    10921 tttactatgc agctgtactc caaattgagt cggcgcagtc ggtgaccagt caaccattgg
    10981 gtcattcaaa taggcagtat tttgaagcgc gattgttgta tcacgattac cattcacata
    11041 gtaatttttt aaagtatgtt tccaagttga taaaccagta actgcatctg gtccatcatt
    11101 ggcagtaagc ggctctgcct cagtagatac aaatgtcatt aagaacttat tatcatctgt
    11161 gttgatctct gtagcataat ttgctcgaag ttctgccaaa cgactagctt gttggtccct
    11221 tgataaacta gtatcggcct tgacctcatc aatttcagca atatatttat caataatcgc
    11281 aatacgactt gagaaatcta atgcctctgc cccaccattt gtaacaaaat ctacaggtga
    11341 cattggatgg tactttttaa agttttcaag aaaatctgga tgatcagggt ttaacataaa
    11401 gtttacaaat cgagcggtac catcttcttt tgtaatataa ataacccttg ctgtcttcgc
    11461 tccagggaag tattgaatta gcggcgtacc aacagaatag ttcgttgaaa ttgtagtatc
    11521 tttaatctca ggtgactcaa ttaactcata atttgcaaac tctgctgcat cagaaattgt
    11581 aaaggaatct ccagaaacct cacctaaatg ggcataactt gcaagaagtt cttctgaacg
    11641 tcgttcagtt tctttaacaa gatagctagt aatctgggtt ggtagtattg agggagccaa
    11701 gccagtataa ctgttgtatg aagtggcatc agatgttaaa agtgaatacg caactgaacc
    11761 tttcgcatta ccacctaggc cagaattgat attcacatat ctagcacttg tttgttggcc
    11821 agtcgaatcc gtaaatggca tcgtagttaa cgacatacgg taagtgaatc ttcctagatt
    11881 agtcggtcca tttgcaatag tctggaattg atgacgagaa gtattttcag gaattgctac
    11941 ctcttcaata aatccctttt gtttacttac caaacgtgcg tatattacgc cgtcagaggc
    12001 agtacgatcc attgaaaggg taatatagta atccgtcccg taacgaagat tataagaacc
    12061 atcttgtatt ccagctgatt ttccagcatc tacgtcatca gctgacaaac tataaatcaa
    12121 gaaagtaata cgaccatctg tagggtcaac agcgtatcct gcaggaatac gttgctcttg
    12181 aggttttatc gtggctgttg ccgttgccgc ttctttgttg agaacttctt cattctctgt
    12241 ccagtaacca ggtctgttag ttgcgtcgtt cagatcagta acagttgcca aagcaatctc
    12301 gataacatct gttcccaagt acttacgcaa ttccaaagca agtgcttcaa cgttggtacg
    12361 aacggctgtg atttgtgcat ttagtgcatc taaagtagca actgtatctt gtaatacaga
    12421 cgttgcatct gtagcggcca attttgttgc agctgctaca gccttcaaag cacttgtatc
    12481 ggttgttgta tcagtagcca caatgcggtt accttcattt gccaaaactt ctgcttctga
    12541 agttacttgc tcaagaactt tttttgcttc ttctacagta gcaggtgttt ttacagcttc
    12601 agaaactgtt gcaggtgttg tagctgtgtc ttctgttgat ttagcagttg ctgtttctgt
    12661 tgaagctgtt gtagttgttt cagaagttgc tgctttttct tcagctttct tcacaacttt
    12721 aacagtaaca atgtttgcac cattttcagt cacttcctga gaaactgtag ctgactgacc
    12781 tgctgccaat tcgtaacttt ctggtactac tgctgtgaca gataccgttg cttttgcaat
    12841 tgtttctgtg gtagtaactg aaactgtttc tactgaagcg ttcacaagtg taccatcttc
    12901 agtagcgtac ttaacaatat agccgatttc aactgtacgt tcagaacttg atgaagtcgc
    12961 tgtttctgtt gaagtttctg atgtcgttga ttctgtagca cttgtggatg aagctacgga
    13021 cgtagactct ggggttgtaa ctgtctctgc tgcttgaaca acctgtgtcc cagtaccaag
    13081 tgcaatcgac atcccaagta agacactagc agcaccaaag tggtatttac gaattgagaa
    13141 gtgctgtttc aatccgtacc agtcaaaaga ttttcttcta gaatgatgca ttattatcct
    13201 cctgtattct atgcatagct aaaaataaga taggtaacaa aaaatgcttt cagattacat
    13261 ctccctcatt ttactattag ctaactattt t
//
LOCUS       DATUCG010000064        13169 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.065, whole genome shotgun sequence.
ACCESSION   DATUCG010000064 DATUCG010000000
VERSION     DATUCG010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13169
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.065"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            109..1683
                     /locus_tag="VCA20_001430"
     CDS             109..1683
                     /locus_tag="VCA20_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEP1835240.1"
                     /translation="MTQACIYLRLSRDDGDNVESNSIINQRSLLRDYARNHDFTILEE
                     FMDDGISGLTFNRPDFNRMMAMVNDKTIDTIIVKDLSRFGRDYIETGKYLQRIFPAMG
                     VRFISVNDHYDSMTADTNETHLVMPIKSLINDSYCRDISTKVRSTQKAKRQKGEFIGA
                     FAPYGYRKDVKQRNHLVVDENVRPTIEKIFSLKLDGYSSNAIAGFLNQAGVETPLQRK
                     KACQEKIKGFYGHCHKWDTKMVNRILTNRVYTGTLEQGKQTKLNYKSQKSVAVNPKDW
                     VRVDDTHEAIISKSTFEIANNLLLRDVKHGKKLPDLFAGLLFCSECQAQLLQRKRRYK
                     DKETVQYICSTYNNGRGCSRHAIKREMLFDLVSTFIHQHLDWKSYLIKTVPDYLDKEQ
                     FLEVDFTSLIANRKKYEQLLQSLYLDLDDGLINDTEYQEFQRSYRQKLKQIEDTIIQK
                     KALAQTLYDKLQDKERWLKQLSEIQKDKELNRITLVTLLDRIVIHEDQELTLVFHDMA
                     ELEVLQQFSHREMEVV"
     gene            1683..3299
                     /locus_tag="VCA20_001431"
     CDS             1683..3299
                     /locus_tag="VCA20_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEP1835241.1"
                     /translation="MARTKNRYRKEMIEELPLQTYRTGIYIRLSKERTETWRNKSQSL
                     ETQESLARAFAKEKGLTVTKCYIDYEYSGTNFNRPAYQDMMEDLKKGIINCILIRDLS
                     RLGRNYIEMGRLIDKVFPFMGVRFISINDNLDTLNGLEDKKSFEVEIKNLVNDLYSKD
                     ISKKVTASHIQKAKQGYFIAGFAPYGYKLERREGGNRLVIDALVQPVMVDVFEKLMAG
                     QSINQVTKYLNQQKVATPMAYRQTGERYRTENDTRQWQSANLQRLVQNPAFYGSLIQR
                     RHNKTLTEKDFIQVDDCHEAYITKEEHQTLQKLISERRSIRKQSQPRTTNRYKGLIYV
                     KGQSTQMRRYCKHFSNSKSEYDYYYFMDYLHGSAPSEKQTVYISEQALDKIVLSVVQT
                     EMKRLGTVKNLLPKLEMKRNECLRDCQKKIKKFQRTLASLQAEQSELYLNYRLEAQER
                     GTYLSQKAMITSKIGTLSKEIAHYEAFAMRIEEKHRQQVSWVKYLAKFQNQSVLTAEL
                     LNEVIERIDVEVDKSVTVTFTCLMGGDNHV"
     gene            3292..4728
                     /locus_tag="VCA20_001432"
     CDS             3292..4728
                     /locus_tag="VCA20_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEP1835242.1"
                     /translation="MSKMALYLRLSVDEDGTDESKSITNQRYLLNQYLDQSSDFNHWE
                     RLEFVDDGFSGTSLNRPAFQRLMADVKAGIIRYIIVKDLSRFMRDYLELGNYLENIFP
                     FLGVRFIAINDQYDSLTSEQNGLDIDVPFRNLLNDFYAKDVSEKVKSAMNSMKRSGKN
                     MNWLPPFGYIKDPEDRFKIIIDEAVAPIVRRIFTLYLDGNSYQKIARILNKEQVITPA
                     ERKMQISQARYEKSLRLTREQERNVWSVINVAQILKNEAYKGTYLYNTRTYIRGKHVS
                     LPESEWERIENNHEAIISPDQFEQVARLKAKKVIQKKHSQPPKIPSILTGLIRCEHCD
                     HSLIGHYNHQGYQYFSCRYCKGQGEKMKSCRVDRIEQSIKEKLRQEVVKPRQIASKIR
                     LLREIERLKKQKLSCYQDYKDNQVSRKEYITKKMGIDESVKNLEEQLNEVNKVQLLTK
                     DKLTKEIVCTHIEKVVADCLGFFTVIYK"
     gene            4838..5182
                     /locus_tag="VCA20_001433"
                     /pseudo
     CDS             4838..5182
                     /locus_tag="VCA20_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028182.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
     gene            5380..6192
                     /locus_tag="VCA20_001434"
     CDS             5380..6192
                     /locus_tag="VCA20_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEP1835243.1"
                     /translation="MMKKYIMTGLVLLATICLSACSGNATQEDTSLKDVQEKGKLIVA
                     LNPEFPPFEFRTLVDGKDTIVGADIELAKAIGQELGVEVEFSAMSFDNVLNNVQSGQS
                     DIAISGISATEERAKVYDFSIPYYTSTNKVIINKEDLAQYTSLDSLAEANIGAQKGSI
                     QEQIVKEQLPSSTVIALASNGELINQLKSQKVEAVIFEEPIAKAYVAKNDDLVILDTE
                     IKSSYSDAYAIALPKGSTALKEKVDSVITKLVESGQMDQFVQEGYELSIANN"
     gene            6364..7164
                     /locus_tag="VCA20_001435"
     CDS             6364..7164
                     /locus_tag="VCA20_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEP1835244.1"
                     /translation="MKKILALVATVLAGLTLAACSSTSSQSALDKIKEKGTLVVATSP
                     DYAPFEFQALVDGKNEVVGADIMLAQKIADKLGVKLEVSAMSFDNVLSSVQNGKADIA
                     IAGLSYSEERAKVFDFSESYYQISDVLLIKKDSANSLTSIDAMSGKTLAVQKGSTQES
                     YAQENISQANLISLTLMGEAVNELKSGKVDAILMDSPVAAGYVSQNSDLAVASVEFPT
                     IDENSKVIALPKGSTELKTAIDKVIAEVKASGEFDTFLEKAATYTSVE"
     gene            complement(7292..9130)
                     /gene="glgB"
                     /locus_tag="VCA20_001436"
     CDS             complement(7292..9130)
                     /gene="glgB"
                     /locus_tag="VCA20_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEP1835245.1"
                     /translation="MTEFTDLDLYYMNTGEHTTLYEKMGAHIIKERNKIIGTQFRVYA
                     PNAREVFLVGNFNEWQRSHSMEREIDGVFQLYVDGLKSLEDYKYLIITHDGREIYKAD
                     PYAFFSQVRPDTASTTYNSRYKFKDDIWMYNRVNYDFKEQPVSIYEVHLGSWKQKFAN
                     KNEGGAPVEAFNSYKEITPLLIEHIRENNYTHIELLPITEHPFDASWGYQVTGYYSPT
                     SRYGKPDELKYLVDQCHQAGIGVILDWVPLHFCKDAHGLYQFDGSWLYEYSYEQDREN
                     HQWGTANFDLGKGLTRSFLLSNLKYWLEYFHFDGIRVDALSYLLYWRGETDEDKINHA
                     AIDFIKRVNAMVHTDYKGVLMIAEDSSSFPKVTHPLEDGGIGFDMKWDLGWMNDTLKF
                     VERPAIYRKYHSNEITFGMYYNQNEQFLLPLSHDEVVHGKHSIIEKMNGDYEDKFHLA
                     RVYYSFYFAHPGKKLLFMGNEWGHIREWHEYTEMDWGLLQFPIHHSFYQMMKTLSTFY
                     KEHDAFWKYDYQAYEKGFNWVKIDEEASLYAFSRTSDVQEILTIHNFNDQELFDVHLD
                     LPADSEYKLVFSSNAIPIDTFSLYPDENGARITVPRLTSFYLERVK"
     gene            complement(9203..10648)
                     /gene="glgA"
                     /locus_tag="VCA20_001437"
     CDS             complement(9203..10648)
                     /gene="glgA"
                     /locus_tag="VCA20_001437"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEP1835246.1"
                     /translation="MTKKSVLFVASEGLPFIKTGGLADVIGSLPKELVKQGLDVRVVL
                     PLYKKIAVNNHSDFDYVSSFDVRAGDIQSMANVYSQVVDGVTFYFIEHRDFFERDELY
                     GYDDDAMRFGYFQHATCRLLEALHFFPDVMHTHDWHTAALPFLCRTFYSYREEFRNIK
                     HVFTIHNLAFQGIFHKQALWSALGMDYSYYLDGIARFHDECISFMKLGILYADKVTTV
                     SETYAREILTEEFGENMQHVLELRKYDLVGIVNGIDYDSWDSQTDQYLVRNYSLETIE
                     SKKANKLALQAQFALPQDENVLLIGIVSRLTWQKGFYLLTEVLGHLIQAHVQFVILGN
                     GEEDIENAFNHFKHAYPDKFAFYRGYNEPLAHQIYAASDLFLMPSMFEPCGISQLISM
                     HYGTLPLVRETGGLVDTVAPYNMSTKEGTGFSFGGRDAYNMRQVYDLALQTYYDRPDD
                     WHAMVEQAMKRDFSWTASAEKYIWLYCEISG"
     gene            complement(10657..11772)
                     /gene="glgD"
                     /locus_tag="VCA20_001438"
     CDS             complement(10657..11772)
                     /gene="glgD"
                     /locus_tag="VCA20_001438"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HEP1835247.1"
                     /translation="MLSNTLGIVNIDGNNVHFGDVMKHRGVQAFGFLGRYRLIDFALS
                     NMSNSGITEFQVYMPAEMRSTIQHVGTGKHYNINSKRGTLRLLNSATDPNSVYRHDIN
                     AFTENIHYIENSTKDYVLVAPSYFIYSQDFSKVMDDHIATNADITVLYKNVSDAKENF
                     IGCQTLKFGEDRRVVAFEENHGKYKNRPVSLEAYIMKRTTFIDLIKRANKVSSLYWLK
                     DILRDTVDQYKIIGYAHRDYVACINTTASYFNTQIELIDQDTRKLLFKHDWPIHTQTS
                     DSSPCLYGPLAEVKRSLISNGANINGQVENSVIDRDVIIEEGVVIQNSIILNGVTIKS
                     GANIENAIIDKATKIIHPIDIKGSETSPSYLKPHQII"
     gene            complement(11785..12912)
                     /locus_tag="VCA20_001439"
     CDS             complement(11785..12912)
                     /locus_tag="VCA20_001439"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEP1835248.1"
                     /translation="MAQNKMLAMILAGGRGTRLEGLTKKVAKPAVAFGGKYRIIDFPL
                     SNCANSGIDIVGVLTQYEPVLLNSYVAQSQRWGLDVQGSGVFVLPPSEKIEGFGLYKG
                     TADAITQNIDFIDLHDPEYVLILSGDHIYKMNYDKLLDTHIQKKADATIAVIEVPIKE
                     ASRFGIMNTDEDYRIEEFEEKPENPKSNLASMGIYIFTWKTLKKYLQEDDKLDTSSHD
                     FGHDIIPKYLEDGRTLIAHPFRGYWKDVGTVNSLWESNMDLIDHAGDLDLSDRSWRIY
                     SEDKGSPAQVIGATATVKSAYIDKGAIIDGTVEHSVISTDVQVNQNAVVKNSVILPDA
                     IIGEGVELDYVIVAENIKIADGVKLTGDIDHILLIDKNVTK"
BASE COUNT         4182 a         2386 c         2712 g         3889 t
ORIGIN      
        1 cctgttttaa ttcctcgatt tgggaggacg taagttcttt tgtcgtgaaa ctattatatc
       61 gctatttatg atttttgtta agtataaaca ctgattaagg agataaacat gacacaagcc
      121 tgtatttatt tacgcctgtc acgagatgat ggggataatg tggaaagcaa ttccatcatc
      181 aaccaacgct cacttttgag agattatgcc agaaatcatg atttcacgat tttagaagaa
      241 tttatggatg atggtattag tggcttaacc tttaaccgcc ctgactttaa ccgtatgatg
      301 gcaatggtaa atgataagac aattgatact attattgtta aggacttgtc acgcttcggt
      361 cgtgactata ttgagactgg gaaatacctt cagcgaattt ttcctgctat gggagttagg
      421 tttatctcgg ttaatgatca ctatgatagt atgactgcag acactaatga aacacacctg
      481 gttatgccta tcaaaagtct tatcaatgat agttactgtc gagacatctc aaccaaagtg
      541 agaagtaccc aaaaagccaa acgacaaaag ggagagttta tcggagcttt tgcaccctat
      601 ggctatcgca aagatgtcaa gcaacggaat catctagtgg ttgatgagaa tgtacgccct
      661 actattgaga aaatcttttc tctaaaacta gatggctatt cgtcaaacgc tatcgcaggc
      721 tttcttaacc aagctggagt agaaacacct ctacaacgta aaaaagcctg tcaggaaaag
      781 ataaaaggat tttatggcca ttgccataag tgggacacca aaatggtcaa ccgtattttg
      841 accaaccgtg tctatactgg taccttggaa caaggtaaac agaccaaact caattataag
      901 tcgcaaaaat ctgtagctgt caatccaaaa gattgggttc gagttgacga tacgcatgag
      961 gcaattatct caaaaagcac gtttgagata gccaataatc tcttactaag ggatgtcaag
     1021 catgggaaga agctgcctga cctctttgca ggattattat tttgcagcga atgtcaggct
     1081 cagcttttac aacgaaaacg ccgttacaag gataaagaga ctgttcagta tatttgtagc
     1141 acctacaata acgggcgagg atgtagtcgt cacgctatca agcgagagat gctgtttgac
     1201 ttggtatcaa cgttcatcca tcaacacctt gattggaagt cttatctaat taaaactgtt
     1261 cctgattatt tggacaaaga gcagtttttg gaagttgatt ttacttcttt gattgcgaat
     1321 cgcaagaagt atgaacagtt actccagtcc ttgtacttgg atttagacga cggtttaatc
     1381 aatgatacag aataccaaga gtttcagcga agctatcgcc aaaaactaaa gcaaattgaa
     1441 gacactatca ttcaaaagaa ggcactagct caaaccctgt atgacaaact tcaggataag
     1501 gaacggtggt taaaacaatt gagtgaaatc caaaaagata aagagctcaa tcgtatcact
     1561 ttagtgacct tacttgaccg tattgtcatt catgaggatc aagagttgac cttggtgttt
     1621 catgatatgg cagaactcga agtcctccag cagttttctc atcgagagat ggaggtggtc
     1681 taatggcacg taccaagaac cgctaccgta aggaaatgat agaagagtta ccacttcaaa
     1741 cttatcgtac gggcatttac attcgcctat ccaaagaacg tactgaaacc tggagaaaca
     1801 agtcgcagtc cttagagacg caagaaagtc ttgctagagc ttttgccaag gagaaagggt
     1861 taactgtaac caagtgttac atcgactatg agtattctgg gacaaacttt aatcgacctg
     1921 cctaccagga catgatggaa gacctcaaaa aagggattat caactgtatc ttgattcgag
     1981 acttatcccg cttgggtcga aactacattg agatgggacg actgattgac aaagtattcc
     2041 cctttatggg ggttcgcttt atctcaatca atgacaattt agacacactc aacggattag
     2101 aagataagaa atcctttgaa gtggaaatca agaatttggt caacgactta tattctaagg
     2161 atatttcaaa gaaagttacc gctagtcata ttcagaaagc aaagcaaggt tatttcatcg
     2221 ctggctttgc tccctatgga tataagttag aaagaagaga gggtggtaat cgtttagtca
     2281 ttgatgcgct agttcaacct gttatggtag atgtctttga aaagttaatg gcaggccaat
     2341 ctatcaacca ggtgacaaaa taccttaatc agcagaaagt tgctacgcca atggcttatc
     2401 gccaaacagg agaaaggtat agaacagaga atgatacacg acaatggcaa agtgcgaacc
     2461 ttcagcggct tgttcaaaat cctgcgtttt atggcagtct cattcaacgt cggcataata
     2521 aaaccttaac tgagaaagat tttattcaag tagatgattg tcatgaagct tatattacta
     2581 aagaagagca tcaaacactt caaaaactaa tcagcgaacg tcgttccata agaaaacaga
     2641 gtcaacctcg tactactaat cgttataagg ggcttatcta tgtcaagggg caatcaactc
     2701 aaatgcgaag gtactgtaag catttctcaa attccaaatc agagtatgat tactattact
     2761 ttatggacta tcttcatggt tcagcacctt cagagaaaca aactgtctat atcagtgaac
     2821 aagccttaga taagattgtg ttgagcgtag ttcagacaga gatgaaacgt cttggcacgg
     2881 ttaaaaactt attgcccaaa ttagaaatga agaggaatga atgtcttaga gattgccaaa
     2941 agaaaataaa aaaatttcaa aggactttgg ctagtctaca agctgagcaa agcgagcttt
     3001 atctgaatta ccgccttgag gctcaggaac gagggactta tttgtctcaa aaggcgatga
     3061 ttacctcaaa aattggaact ctctcaaaag aaatagccca ttatgaggct tttgcgatga
     3121 gaattgagga gaaacatcgt caacaggtca gttgggttaa gtatcttgct aaattccaaa
     3181 atcaatcagt tttaacggca gaactgctta atgaagtgat tgaacggata gatgttgaag
     3241 tggacaagtc tgtgacagta acttttacct gcctgatggg aggtgataac catgtctaaa
     3301 atggctttat accttcgtct ttccgtcgat gaagatggga cagacgaaag taagtccatt
     3361 accaaccaac gctatctttt gaatcagtac cttgatcagt catcagactt taatcattgg
     3421 gaacgcttgg aatttgtaga tgatggtttt tcaggaacaa gtctcaatcg acctgccttc
     3481 caacgcttga tggctgatgt taaggcggga attatccgtt atatcattgt taaagatttg
     3541 tctcgtttta tgcgtgatta tctggaacta gggaattatt tagaaaatat tttccctttt
     3601 ctcggagttc gcttcatagc tataaatgac caatatgata gtttgacatc tgaacaaaat
     3661 ggactggata ttgatgttcc gtttagaaac ctcctcaatg atttctatgc caaggatgtt
     3721 tctgaaaagg ttaagagtgc gatgaacagc atgaaacgct ctggaaaaaa catgaattgg
     3781 ttgccaccat ttggctatat taaggatcca gaagaccgat tcaagattat cattgatgag
     3841 gcagttgcac caattgttag acgtattttt accttgtacc ttgatggtaa tagttatcaa
     3901 aagattgctc gcatcttgaa taaggaacaa gtcattaccc ctgcagagcg aaaaatgcaa
     3961 attagtcagg cacgttacga aaagtctctc cgactaacaa gagagcaaga gagaaatgtg
     4021 tggagcgtaa taaatgttgc tcaaattttg aagaatgaag cctataaggg aacttatctc
     4081 tataatactc gaacctacat aagggggaag catgtttctc taccagaatc agagtgggag
     4141 cgaattgaga ataaccatga agcgattatt tcaccagacc aatttgaaca agttgctcga
     4201 ttaaaagcta agaaggttat tcagaaaaaa cactctcagc ctcctaaaat tcctagtatt
     4261 ttgacaggcc tgattcgttg tgaacactgt gaccattctt taattgggca ctacaatcat
     4321 caaggttacc aatatttttc ctgtcgttat tgtaaaggac aaggtgaaaa aatgaagtcc
     4381 tgccgagtag ataggattga gcaatcaatc aaggagaagc ttagacaaga ggtggttaag
     4441 ccccgtcaaa ttgcttctaa gattagattg cttagagaaa ttgaacggtt gaagaaacaa
     4501 aaactatcct gttaccaaga ttacaaggat aaccaggtgt ccaggaaaga atacattaca
     4561 aagaaaatgg gcatagatga atctgttaag aatttagaag aacagctaaa cgaagttaat
     4621 aaagttcagt tgttgactaa agataagtta accaaagaaa tagtttgtac tcatattgaa
     4681 aaagtagtcg cagactgcct cggatttttt acagttatct ataaatagtt gaggaagaga
     4741 cttgaatcct atcaagtctc tttctcgata ggttgctttg tgatacaatt ggtgtgaaat
     4801 ctagtaaata aatagtgtca gttacttgac ataagaggga catattgaag aaggcgagaa
     4861 tttccatgat tcggttgtta gagaagtgca agaagaaacg ggtttaacag tcactgatgc
     4921 tcgcttggtt ggtcttaaac attggccgga taaggaaggg catcgttaca ttgtatttcc
     4981 ctataaggca acagagttta caggaactat ccaatcaact gaagaaggcg aggtgcgttg
     5041 ggtcgagaag tcggacctgc ctcagatgga tttggcctat gatttgctag aaatactgaa
     5101 agtcatggat gagccagatt tgtccgagtt cttttatact agaaaaaatg acgatggaga
     5161 gtgggacaag aattttaggt agaagctaga aatagcttct tttttatgtt gtttgaataa
     5221 aaatacaaca aataagttga caaatgtata aaaatgacat aaaatgtttt tatctttcaa
     5281 aaaaaggaga aaaaatatgc atttcactac aatgaaagtc cgatgtttta tataaaaaac
     5341 ggtataagta tgcacgggat tattttaaag gagaatatga tgatgaaaaa atatattatg
     5401 acgggattgg ttttactggc aacaatatgt ctaagtgctt gtagtggaaa tgctactcaa
     5461 gaagatactt ctttgaagga tgttcaggaa aagggaaaac taattgttgc attgaatcca
     5521 gaatttccac catttgagtt tagaacactg gttgatggca aagatacaat cgttggagct
     5581 gacattgagc ttgcaaaagc cattggtcaa gaattgggtg tagaagtcga attttcagcg
     5641 atgagttttg ataatgtttt gaacaatgtc cagtcagggc aatcggatat tgccatttca
     5701 ggaatttcag ccactgaaga gcgtgcaaaa gtgtatgatt tttccattcc ttactacacc
     5761 tcaaccaata aagttattat aaataaagag gatcttgcac aatatacatc gcttgattct
     5821 ctagctgagg caaatatagg ggcacaaaaa ggttctattc aagaacaaat tgttaaagaa
     5881 cagcttccgt cttccacggt tattgctcta gcttcgaacg gcgagctgat taatcaatta
     5941 aaatcacaga aggtagaagc agttattttt gaagagccga ttgcgaaagc ctatgttgcc
     6001 aaaaatgatg acttggttat tttagatact gagataaaaa gtagttattc agatgcctat
     6061 gctattgcct tgccaaaagg aagtacagct ttaaaggaaa aagttgactc tgtcattaca
     6121 aaactagtgg aatctggaca gatggatcaa tttgttcaag aaggctatga attatccatt
     6181 gctaacaatt aaattttata catttaaagt gaataaaaat aacattttct tgacaagtgt
     6241 ataaaaatga attaaaatga gaatacattc tgaaaaactg caagaaaata caatgaaaat
     6301 caatgagctt gtgtttttta gaataagtat tttattaaaa taaagagaag gtatttagga
     6361 aatatgaaaa aaatcttggc attagtagca acagttttag caggactgac attggcagct
     6421 tgttcttcga cgtcatcaca atccgcatta gataaaatta aagaaaaagg tacattggta
     6481 gtggcaacta gtccagacta tgctccattc gagtttcagg cactagttga tggaaagaat
     6541 gaagttgttg gtgcagatat tatgttggct caaaaaatag ccgacaaact tggggtcaag
     6601 cttgaagttt cagcaatgag ttttgacaat gtattgtcta gcgttcaaaa tggcaaagct
     6661 gatattgcaa ttgctggtct atcttattcg gaagagcgtg caaaagtttt tgatttctct
     6721 gaaagttact atcaaatttc tgatgtatta cttatcaaaa aagatagcgc taattcactc
     6781 acttcaattg atgctatgag cgggaaaact ctcgcggtac aaaagggttc aacacaagaa
     6841 tcctacgcac aagaaaacat tagtcaagct aacctaattt ctttaacctt gatgggtgaa
     6901 gctgtaaatg agcttaaatc aggaaaagtt gatgctattt taatggattc tccagttgct
     6961 gctggttatg tttcacagaa ttcagattta gccgttgcat ccgttgaatt tccaacgatt
     7021 gatgagaact cgaaagtcat tgctttgcca aaaggtagta cagaactaaa aactgcgata
     7081 gacaaggtta tcgcagaagt aaaagcaagt ggtgagtttg atactttcct tgagaaggct
     7141 gcaacatata caagtgttga ataagagaaa tgaacaatca tcggaaacga tggttgtttt
     7201 tgcatgcaaa aaagaatgga gtaagtgagc cacaccccaa aagttagaca gaaaatttgg
     7261 atcggatcaa tactccattt tttatagata attatttcac acgctctaga taaaaactgg
     7321 tcaaacgtgg tacagtaatt ctagctccat tttcatcggg atagaggcta aatgtgtcta
     7381 ttggaatagc attagatgag aagacaagtt tatattcgct gtcagctggc aaatccaagt
     7441 gtacatcaaa tagttcttga tcattaaagt tatgaatagt caagatttct tgcacatcac
     7501 tagtgcgaga aaaagcatac aggcttgctt cttcatctat ttttacccaa ttaaatcctt
     7561 tttcataggc ttggtagtca tatttccaga aagcatcatg ttctttatag aaagtagata
     7621 gtgttttcat catttgatag aaagaatggt ggattggaaa ttgtagcagt ccccagtcca
     7681 tttctgtata ttcgtgccat tcacggatat gaccccattc atttcccatg aagagtaatt
     7741 tctttccagg atgggcaaag taaaagctgt agtagactcg agccagatgg aacttatctt
     7801 cgtagtcacc attcatcttc tcgataatgg agtgcttccc atgaacaact tcgtcgtgcg
     7861 aaagtggtag taagaattgc tcgttttggt tatagtacat accaaaagtg atttcatttg
     7921 agtgatattt tcgataaata gcagggcgtt ccacgaattt tagtgtatca ttcatccagc
     7981 ccagatccca tttcatatca aatccaatac cgccatcttc tagaggatgt gtgactttag
     8041 ggaagcttga agagtcttca gcaatcatca agactccctt atagtcggta tggaccatgg
     8101 cattgacacg tttgataaag tcaattgcgg catgatttat cttatcctca tctgtttcac
     8161 cacgccaata gaggagataa gatagtgcat caacacggat gccatcaaaa tggaaatatt
     8221 ccaaccagta ttttaaatta gaaagaagga aagaacgagt cagtcctttt ccaaggtcaa
     8281 aattagcagt cccccactgg tggttttcac ggtcttgttc gtaggagtat tcgtatagcc
     8341 aactaccatc aaactggtag agcccatgag catctttaca gaaatgaagt ggtacccagt
     8401 caagaatcac tccgattcct gcttggtgac attggtcaac taaatactta agttcatctg
     8461 gcttgccata gcgactagtt ggactgtagt agcctgttac ctgatagccc caagacgcat
     8521 cgaaaggatg ctcggtaatt ggcaataatt caatatgtgt gtagttattt tccctgatat
     8581 gttcgatgag aagtggagta atttccttat aactattgaa agcttctaca ggagctcctc
     8641 cttcattttt attagcaaat ttttgcttcc acgaacctaa gtgcacctcg tagatggata
     8701 ctggctgttc tttaaaatca tagttaacac gattatacat ccagatgtca tctttgaatt
     8761 tataacgcga attgtaggtt gtagaggcag tgtctggacg aacttggctg aaaaatgcat
     8821 aagggtcagc cttatatatt tcacgcccat cgtgtgtgat aatcaggtat ttataatctt
     8881 caagggattt taatccgtct acataaagtt gaaatactcc atcaatttca cgctccatag
     8941 agtgactgcg ttgccactcg ttaaagttgc caactaagaa tacttctctg gcatttgggg
     9001 catagacacg aaattgagtt ccgataattt tgtttcgttc ttttataata tgagcaccca
     9061 ttttttcgta aagagtggta tgctcaccag tattcatata atataaatct aggtcagtaa
     9121 attctgtcat agcaatctcc attgcagaaa aggagtggac taggggcaca ctccgattca
     9181 gtttattttt tcatcttatt tcctaaccac taatttcaca gtagagccag atgtatttct
     9241 ctgccgaagc tgtccaacta aaatcacgct tcatagcttg ttcaaccatg gcatgccagt
     9301 catcgggacg gtcgtagtag gtttgaaggg ctaaatcata gacttgtcgc atattgtaag
     9361 cgtcacgtcc tccaaagcta aaaccagttc cttctttggt agacatattg tatggtgcta
     9421 cagtatcaac caagccacct gtttcacgaa ctagagggag tgttccgtaa tgcattgaaa
     9481 tcaactggct aatgccgcat ggttcaaaca tagaaggcat gaggaagaga tcgcttgctg
     9541 cataaatttg gtgggcaagt ggttcattgt aaccacggta gaatgcaaat ttatcaggat
     9601 aagcgtgttt aaagtgatta aaggcatttt caatgtcttc ctcaccattg cctaagataa
     9661 caaattggac atgggcttga ataagatggc caagtacctc ggtcaagagg tagaaccctt
     9721 tttgccatgt caaacgagag acgataccta tcaataggac attctcatct tgtggaaggg
     9781 caaattgtgc ttgcagggcc agtttattag cctttttact ttcaatcgtt tcaagactat
     9841 aatttctcac aaggtactga tctgtttgac tatcccaact gtcatagtcg ataccattga
     9901 caataccaac caagtcatat ttgcgcaact ctagtacatg ttgcatgttt tcaccgaatt
     9961 cttccgttag gatttcgcga gcataagttt cagaaactgt agtaaccttg tcagcgtata
    10021 ggataccaag cttcatgaag ctgatacatt catcatgaaa acgtgcaata ccatccaaat
    10081 agtagctata gtccatgcca agcgctgacc aaagtgcttg tttgtggaaa ataccttgaa
    10141 aagccaagtt atgaatagtg aatacatgct tgatattgcg gaattcttcg cggtagctat
    10201 agaaagtacg gcaaaggaat ggaagggcag cagtgtgcca gtcatgtgtg tgcatgacat
    10261 ccgggaagaa atgaagtgct tctaacagac gacatgtagc atgttggaaa tagccaaaac
    10321 gcatggcatc atcgtcgtat ccatatagtt catcgcgttc aaagaaatcg cggtgttcaa
    10381 taaaatagaa tgtcactcca tcaacaacct gactgtaaac atttgccata gattggatgt
    10441 cgccagcccg cacatcaaaa ctggatacgt aatcgaaatc agagtgattg tttactgcaa
    10501 tttttttgta gagtggtaga acgactcgga catctaaacc ctgttttacc aattcttttg
    10561 gtaaagagcc aataacatca gccaatccgc cagtcttgat gaatggaagt ccctcggatg
    10621 ctacaaagag aacagatttt tttgtcatgt tagctcctaa atgatttggt gtggtttcaa
    10681 gtatgaaggc gaagtttcgc ttcccttgat atcaattggg tgaataattt tagttgcttt
    10741 atcgataatt gcattttcaa tattcgcccc gctcttgatt gtcacgccat tgagaatgat
    10801 tgagttttgg ataacaacac cttcttcaat gataacatcg cggtcaatga ctgaattttc
    10861 gacttgacca ttgatattcg ctccatttga aattaggcta cgtttgacct ctgccaatgg
    10921 accatacaaa catggtgagc tatcacttgt ttgggtatgg attggccaat catgtttgaa
    10981 gaggagtttg cgggtatctt ggtcaatcaa ttcaatttga gtattgaagt aggaagcggt
    11041 cgtattgata catgcgacat aatcacgatg tgcatagcct ataatcttgt actggtcaac
    11101 tgtatcacgt aaaatatctt ttaaccagta gagagacgac actttgttag cacgtttaat
    11161 caagtcgata aatgtagtcc gtttcataat gtaggcttca agggaaacgg gacggttttt
    11221 atatttaccg tggttttcct caaaagctac gacacgacgg tcttcaccaa atttgagagt
    11281 ttggcagccg atgaaatttt ctttagcatc tgaaacgttt ttataaagaa cggtaatatc
    11341 agcattcgtt gcgatatggt catccatgac ttttgaaaaa tcctggctat agatgaagta
    11401 agatggagca actagcacat aatcttttgt agaattttcg atgtagtgga tgttttctgt
    11461 gaaggcatta atatcgtgac gatagacact atttgggtct gttgcgctat tgagcaagcg
    11521 aagggtaccg cgtttgctgt tgatattata gtgttttcct gtaccaacgt gctgaattgt
    11581 tgaacgcatt tcggcaggca tgtatacttg gaattctgta attccagagt ttgacatatt
    11641 ggatagagca aagtcaatta ggcgataacg acctaagaaa ccgaaagctt gtacgccacg
    11701 gtgcttcatc acgtcaccaa aatgtacgtt gtttccatca atatttacaa ttccgagagt
    11761 attacttagc atagcagtcc tttcttactt ggttacattc ttgtcgatca agaggatatg
    11821 gtcgatgtct cctgtgagtt taactccgtc ggcaatttta atattttcag cgacgatgac
    11881 gtagtcaagc tcaacacctt cgccgataat agcatcaggt aggatgacag aattttttac
    11941 tacggcattt tggttgactt gcacatctgt tgaaataaca gaatgttcta cagtaccatc
    12001 gatgattgcc cctttatcaa tataggcaga tttaaccgtt gcagttgcac cgatcacctg
    12061 agcaggtgag cccttgtctt ctgagtagat tctccatgag cggtcagaaa ggtccaaatc
    12121 tccagcgtga tcaatcaagt ccatattgga ttcccagagg ctgttaacgg tacctacgtc
    12181 tttccaataa ccacggaatg gatgggcaat gagagtgcgg ccatcttcta agtatttcgg
    12241 aatgatgtcg tgcccaaaat cgtgagatga agtgtccaat ttgtcatctt cttgaaggta
    12301 ctttttcaag gtcttccaag tgaagatata gatacccatt gatgctaaat tgctcttagg
    12361 attttctggt ttttcttcaa attcttcaat acggtaatct tcgtccgtat tcatgatacc
    12421 aaaacgtgaa gcttctttga tcggaacttc gataaccgca atcgtggcat ctgctttttt
    12481 ctgaatatgt gtatcaagga gtttgtcgta gttcatctta tagatgtggt caccagacaa
    12541 aatcaatacg tactctggat cgtgaagatc aatgaaatca atgttctgtg taatagcgtc
    12601 ggctgtacct ttgtaaagtc cgaaaccttc gattttttca ctcggtggta atacaaagac
    12661 accagatcct tgtacatcaa gtccccaacg ttgagactgt gcaacgtatg agttcaagag
    12721 aacaggctca tactgagtca aaactccgac aatatcaata cctgagttgg cacagttact
    12781 gagaggaaag tcaataatgc ggtactttcc gccaaatgcg accgctggtt tggcgacttt
    12841 ttttgtcaac ccttctagac gtgttccacg tccaccagca agaatcatag ctaacatttt
    12901 attttgagcc atctaaccac tccttaatat atatgataca tgtattatag catagagtac
    12961 gaaaaaatga aagcgtttcc ctaaagaaaa gtgaattatt ttcatttttc ttttcaacgt
    13021 ctttcataaa tgtatcataa catattgtat tttgtttatc tatacccgtt gtgctagtta
    13081 attctagcta aataaaaaca ctttgcgtat cataaactca aaacaaccac gaaatgaggg
    13141 aatgatatgc aaagccaaca ttattatct
//
LOCUS       DATUCG010000065        12778 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.066, whole genome shotgun sequence.
ACCESSION   DATUCG010000065 DATUCG010000000
VERSION     DATUCG010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12778
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.066"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            <1..1620
                     /locus_tag="VCA20_001440"
     CDS             <1..1620
                     /locus_tag="VCA20_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEP1835249.1"
                     /translation="SFSPEDKLTPEEINRIGHETIKELTGGNFRFIVTTHTDRDHVHN
                     HILINSVDLNSDKKLKWDYAQERNLRLISDRLAKESGAKIIPPNRYSHEKFETYRKTN
                     HKFELKQRLYFLMENSKSFEDFLAKAPALNVEMDFSRKHARFLMTDRNMKQVTRGDQL
                     NKRQPYTKDYFREQFAKRGIEERLEFLLPKARSLEHLVKMAEQLNLNISPKQKHVVFT
                     LSENGRSIAIKNEKLSAKCLYDVQFFDDYFSKEKELPDVSLETLVSDFEKYEEEKNKD
                     TLSVEELWSAYTDFKEKRDQVQEFEVALAGHQIDKLVKDGLFVRINYGIKKDGLVFIP
                     NRNLDIHDNEQGKFYHVFIRETAQFFIYNIENSDLNRYMRGRELIRQLTHDSKHIPKR
                     RRSTRTSLKDKILELNLLIELDINNQSYQEVKDELINEIAQLDLTITDLQDKVAHLNK
                     VAEVLLNLNNSDPENRRLARYDYAKMNLTPAIKLEEVEKEISENQDTMTKWIVDYEYK
                     VRSLEILVQTLSHSLSSSDHRDRPSKNELGL"
     gene            complement(1556..1771)
                     /locus_tag="VCA20_001441"
     CDS             complement(1556..1771)
                     /locus_tag="VCA20_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835250.1"
                     /translation="MNNYLKNVRDYSELATIIIVGQKIDYEELFKNHYRVFGVIDTTN
                     NKSLTFIRDQVHFYLDGLYGLKKKESD"
     gene            2150..2491
                     /locus_tag="VCA20_001442"
     CDS             2150..2491
                     /locus_tag="VCA20_001442"
                     /inference="COORDINATES: protein motif:HMM:NF016296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator"
                     /protein_id="HEP1835251.1"
                     /translation="MNFMIIKVKDETKKVLLSDILYIISHPSKPHYIQIVTEKEIYDC
                     LQKLQSIEELYPECFIRCHRSSLVNRSKIKVINSREKRIFLGEKGEHCLTFSRRRYQD
                     ILHQWINKGGM"
     gene            2493..3233
                     /locus_tag="VCA20_001443"
     CDS             2493..3233
                     /locus_tag="VCA20_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEP1835252.1"
                     /translation="MNIFVLEDDFKQQARIEKIITKLLEKHNIVPKKFEISGKTHQLL
                     HAIEEKGVHQLLFLDIEIKEDELKGLQVAKQIREIDPYASIVFVTTHSEFMPLSFRYQ
                     VSALDYIDKELGVTEFESRIEAALLHTHNKDNSAVSDDSFYFKSKYAQVQYPFNEVYY
                     IETSPRPHRVILYTKTDRMEFTAGLSDILKQDKRLVQCHRSFAINPRNVVKVDRQEKQ
                     VYFPNGSTCLVSRQKLESTLAVIDRLHR"
     gene            3244..4587
                     /locus_tag="VCA20_001444"
     CDS             3244..4587
                     /locus_tag="VCA20_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018164876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEP1835253.1"
                     /translation="MSVLNYIIEAIVSLGSLIVLFFSVNRLKYSIRSILLVILLRLSV
                     AALFATLSQLFSSQFFSYFDTPLYGFLLAMILLRPLPKTLLIFYGLLPFTLENLFYRM
                     VLYFIFPLFGQTPGIIEDTPTLVFVNLLSTLSVLFFLGWLRYDFVKLRTDNLAAEDKR
                     ILYLTNWVMFAYYLLMQTLTYLEYDIGLPTMAYRQFILVTYLVIFMGIIKQLDFHLRQ
                     KLQDQLDFQQDLQLKSLEDYSKQVENLYREVKGFRHDYANLMTTLRLAIEDNDIEQIR
                     DIYQTVLKDSSKEFRTSKYDIGRLVNIDNSALKSLLASKFLQANDNNVSVSLEVPEVI
                     KPQGMELVDFITIVSILCDNAIDAAIDAATPKVTIAFLTVNDKQMFIIENNTKEERVD
                     LSELYAFGHSSKGEGRGIGLYNVMKILERYPLISLNTTSKAHLFGQSLEIDLSSK"
     gene            complement(4636..4791)
                     /locus_tag="VCA20_001445"
     CDS             complement(4636..4791)
                     /locus_tag="VCA20_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComC/BlpC family leader-containing
                     pheromone/bacteriocin"
                     /protein_id="HEP1835254.1"
                     /translation="MTKQTKSLTHFSELTSSELHRITGGDWWSDLIRFFPRHDKIAGD
                     NNIHKLG"
     gene            complement(4890..6251)
                     /locus_tag="VCA20_001446"
     CDS             complement(4890..6251)
                     /locus_tag="VCA20_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="HEP1835255.1"
                     /translation="MNPNLFKSAEFYQRRYHNFATLLIVPLILLVGFLLTFSLFAKKE
                     VTVTSRGEITPTTIIASIQSTSNNPITTNNLANNKLVKKNELIIQYAETMESSQKQAL
                     ETQLATLNRQKTGLETLKESLAQGTNLFTGEDEFGYINTFNNFITQSQDIELGISKIN
                     AEVSNQAALAGNTIAAIDTQINQIYHQIAEYEELRQAITNRQTGLSAGNPHQATLNNF
                     LAQSQQDSHTGLTDQYLSQINQSISGLESSIASLNIQRAGTGSVATYDNSFVTKVEVL
                     RTQFLQSASQQLTTLETQITELAAQLEQASVRLENNTITAPETGVIHLNSEFEGKTLI
                     PSGSEIAQIYPDINQTKEFLITYYVTSEHVALLKEEQDVRLSLEKVGNQSITVIGKIK
                     SIDKTATKTEEGNLFKVTALAQLSDKHGNIIQYGLQGRVTSIIAKKTYFNYYKDKILS
                     NHD"
     gene            complement(6263..8416)
                     /locus_tag="VCA20_001447"
     CDS             complement(6263..8416)
                     /locus_tag="VCA20_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_125458604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEP1835256.1"
                     /translation="MTAYKRTFVPQIDARDCGVAALASIAKFYGSDYSLAHLRELAKT
                     NKEGTTALGIVKAAKEMGFETRAIQADMSLFDMDDVPYPFIVHVNKEGKLQHYYVVYH
                     NKKDHLIIGDPDPTVKVTKMTKERFASEWTGVAIFLAPEPSYKPHKDKKNGLMSFLPL
                     IFRQRALIFYIVLASLLVTLINIGGSYYLQGILDEYIPNQMKSTLGIISIGLIITYIL
                     QQMMSFSRDYLLTVLSQRLSIDVILSYIRHIFELPMSFFATRRTGEVISRFTDANSII
                     DALASTILSFFLDVSILTIVGGVLLVQNTNLFLLSLISVPIYIIIIFAFMKLFEKMNN
                     DVMQSNSMVSSAIIEDINGIETIKSLTSEESRYQKIDSEFVDYLDKSFKLSKYSILQT
                     TLKQGAQLILNIVILWFGAQLVMNGHISVGQLITFNTLLSYFTNPLENIINLQTKLQS
                     AKVANNRLNEVYLVESEFSNSQTLTDSQFLTGDIRFEDLSYKYGFGRDTLSDINLTIK
                     QGDKVSLVGISGSGKTTLAKMIVNFFEPYKGRITINNNDLKLIDKKTLRQHINYLPQQ
                     AYIFSGSILENLTLGANQLISQEDIIRACEIAEIRQDIEQMPMGYQTELSDGAGLSGG
                     QKQRIALARALLTKAPVLILDEATSGLDVLTEKKVIDNLMAMADKTIIFVAHRLSISE
                     RTNQVIVLDQGKVIETGSHHELMTKQGFYHHLFSK"
     gene            8873..9088
                     /locus_tag="VCA20_001448"
     CDS             8873..9088
                     /locus_tag="VCA20_001448"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835257.1"
                     /translation="MLELNVEKLETISGGGNVNDCVNGILASAGVLAIAATGPTGWLA
                     LAALGGAAGTAGFATGWSCTALYYNGD"
     gene            9097..9315
                     /locus_tag="VCA20_001449"
     CDS             9097..9315
                     /locus_tag="VCA20_001449"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835258.1"
                     /translation="MMIAEIKDDILEKVSGGGNVSDCFLSASGALGTIAGAGAVTGPI
                     GWGVALVSAAGFGFLTGYNCTKLYFEGD"
     gene            9648..9857
                     /locus_tag="VCA20_001450"
     CDS             9648..9857
                     /locus_tag="VCA20_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComC/BlpC family peptide pheromone/bacteriocin"
                     /protein_id="HEP1835259.1"
                     /translation="MNTKTMEQFNMLNADTLATIEGGGIFIGFRWVCTDGKKSAWHMF
                     KGTAQDNANNYMLRNPGVTCTVDHA"
     gene            9929..10791
                     /locus_tag="VCA20_001451"
                     /pseudo
     CDS             9929..10791
                     /locus_tag="VCA20_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677582.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            10788..11951
                     /locus_tag="VCA20_001452"
     CDS             10788..11951
                     /locus_tag="VCA20_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835260.1"
                     /translation="MRTILSFELTKLLCKKSALGAVTVALLALLGLFYTNFFVGQISG
                     YSSDKVNGKAAVAINRQIAEEHTGYLSDDLMSRILNDYAKNQPDLKKKGVYSVVSHYA
                     ISELIPDSNEMLIAINQTNEVLQFDNIELKSQEQLGSHFPLEELKLGNFAPWNQLFLV
                     SNSSYILILLLSVYLSAPLFSGDFSKKMNPLLLTTKYGRSKLTVAKLTASLLISTSLF
                     VVFNAIILAVFAWYFGFSGWDTSVQLNLYWIVPFENIMAFPEKLTLLSTFVNLIVFQF
                     IGLVFITAVNALVSSKTKSPLTTFAVSAGTIFVPSFLLQVFQGGLIHKLLTIISVPTS
                     NTAGLMLQLSHQGPQGFFLDHFWTNGGLIIGFRLLIVGLFFYRTFKIITKVQR"
     gene            12300..12380
                     /locus_tag="VCA20_001453"
     CDS             12300..12380
                     /locus_tag="VCA20_001453"
                     /inference="COORDINATES: protein motif:HMM:NF015035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComC/BlpC family leader-containing
                     pheromone/bacteriocin"
                     /protein_id="HEP1835261.1"
                     /translation="MNTKKMERFNTLNSEALATVQGGEGG"
BASE COUNT         3766 a         2378 c         2705 g         3929 t
ORIGIN      
        1 tctttttctc ctgaagacaa gctgacacca gaagagatta accgcattgg ccacgagacc
       61 atcaaggaat tgacaggagg caattttcgt ttcatcgtca caacccatac agatagagat
      121 catgtgcata atcatatcct gataaactct gttgacctca attctgataa gaagctcaag
      181 tgggactatg cccaggagag aaatcttcgc ctgatttctg accgcttggc gaaggagtca
      241 ggggcaaaga ttattccgcc caatcgctat tcgcatgaga agtttgaaac ttatcgcaag
      301 accaatcaca agtttgaact caagcagcgg ctctattttc tgatggagaa cagcaagagc
      361 tttgaagatt ttttggccaa ggctcctgct ctaaacgtgg agatggattt ctctcgcaag
      421 cacgcccgct ttctgatgac cgaccgcaac atgaaacagg tcactcgtgg cgaccagcta
      481 aacaaacgtc aaccctacac caaggattat tttcgggagc agtttgcgaa acgaggtatt
      541 gaagagcgac tggagtttct tttacccaag gcaaggagcc ttgagcattt ggttaaaatg
      601 gcagagcagc tgaacctgaa catttctccc aaacagaaac acgttgtctt tactttatca
      661 gagaatggca ggagcatagc cataaaaaat gagaagctga gtgccaagtg tctttacgat
      721 gtccagtttt ttgacgacta tttttctaaa gagaaagagc tcccagatgt tagcttggaa
      781 actcttgtgt cagattttga aaagtacgag gaagagaaaa acaaagatac gctgtctgtt
      841 gaagaactgt ggtcagccta tacagatttc aaagaaaaac gagaccaggt ccaggagttt
      901 gaagttgcat tagcggggca ccagattgac aagttggtca aggacggtct ctttgtcaga
      961 atcaattatg gtatcaaaaa agacggcttg gtctttatac caaaccgcaa cttggatatt
     1021 catgataatg aacaaggcaa gttctaccat gtctttatcc gagagaccgc tcagtttttt
     1081 atctacaata tagagaactc tgacctcaac cgctacatgc gaggacggga gttaatccgc
     1141 cagctcactc acgacagcaa gcatatccct aaacgcagac gatcaaccag aaccagcctc
     1201 aaagataaaa tcttagagct caatcttctt attgaactag atatcaacaa ccaatcttat
     1261 caggaagtca aagacgaact catcaacgaa attgcccagc tggatttgac cattacagat
     1321 ttgcaggata aggttgctca tctgaataag gtcgcagaag tcctgctcaa tctcaacaac
     1381 tcagacccag aaaacagacg gttagctagg tatgattatg ctaagatgaa tctgacaccg
     1441 gccattaaac tggaggaggt ggagaaagaa atctcagaaa atcaagacac catgacgaaa
     1501 tggatagttg attacgaata taaggttagg agcctggaga ttttagtcca gacacttagt
     1561 cactctcttt cttcttcaga ccatagagac cgtccaagta aaaatgaact tggtctctga
     1621 taaatgttaa tgatttattg tttgtcgtat caatcacgcc aaatacccga taatgatttt
     1681 tgaagagttc ttcataatcg atcttttggc caacaataat gatagtcgct agttctgaat
     1741 aatctcgtac attctttaag taattgttca tcttctctga tgataaaaac ttagatttta
     1801 tgataaaata agtagtagga ttatcgtact tatacggata ataagagaaa gatagtttga
     1861 tttttgaata aatctgatga ctgattaagt aacctttaat gacgattgtg ataaaaatac
     1921 ttaatgggga ataaggaaag atttcaatca tagtatcctt tctttttcaa tgatttagga
     1981 atttaattat atcagttatt gttggtttag tgagcttcat ttcctgaata gacctctaac
     2041 tttcctaaat ggcaaaatct taccattcaa gacgtttgga taaccattta ggatttgaag
     2101 gaaatatgat gttgttccta tgctatggtg ttcataggag gatataccta tgaactttat
     2161 gattatcaaa gtcaaggatg aaacaaagaa agtactgctg tcagatattc tatatatcat
     2221 atcgcatcca tctaaaccac attatattca aattgtgact gagaaagaga tttatgattg
     2281 cttacaaaag ctacaaagta tagaggaatt gtatccggaa tgttttatta gatgccatcg
     2341 gtctagtcta gttaaccgtt caaaaattaa ggttatcaat tctcgagaaa aacgtatttt
     2401 tcttggagaa aaaggagaac attgccttac tttttcaaga cgtcgttatc aggacatact
     2461 gcatcagtgg attaacaaag gaggaatgta gcatgaatat ctttgtgttg gaagatgatt
     2521 ttaagcaaca agcgagaatt gagaagatta ttactaagct gttagaaaag cacaacatcg
     2581 ttccgaaaaa atttgaaatt tcaggaaaaa ctcatcagct acttcatgca atagaagaaa
     2641 aaggtgtaca tcagctctta tttttagata ttgagattaa ggaagacgaa ctaaaaggac
     2701 ttcaagtcgc taaacaaatt agagagattg acccttatgc tagtattgtt tttgtgacaa
     2761 cacactccga gtttatgccc ttatctttca ggtaccaggt atctgccttg gactatattg
     2821 acaaagagct tggagttacc gaatttgaat cgcgaataga agcagcttta cttcatactc
     2881 acaataagga caatagcgcc gtatcggatg attcattcta ttttaagtcc aaatatgctc
     2941 aagtacaata cccattcaat gaagtgtact acatcgagac ttctccccgc cctcatcgtg
     3001 ttatccttta taccaagaca gaccgaatgg agttcacagc aggtttgtca gacattttga
     3061 aacaagataa acgacttgta caatgccatc gttcgtttgc aatcaatccg aggaatgttg
     3121 ttaaggttga tcggcaagaa aagcaggtct attttccaaa tggctctacc tgtcttgtct
     3181 caaggcaaaa gctagaatca actttagctg ttattgatag actgcataga taaaggagaa
     3241 aggatgtctg ttttaaacta tattattgaa gccattgttt cattgggttc actcattgtt
     3301 ctctttttta gtgttaaccg cttaaagtat tccatccgtt caatcctctt ggtcattctg
     3361 ctcagattat ccgttgcagc ccttttcgcg actttaagtc agttattttc aagtcaattc
     3421 ttctcttatt ttgacacccc tttgtatggt tttctcctag ccatgatttt gctgcggccc
     3481 ttaccgaaga cccttttgat tttctatggt ctactgccct ttaccttaga aaatctcttt
     3541 tatcgtatgg tcctttattt tattttcccc ttatttggtc agaccccagg tatcatagaa
     3601 gacactccaa ctttagtttt cgttaatctc ctgtctacat tgtctgttct atttttctta
     3661 ggttggttaa gatatgattt tgtcaagtta agaacggaca atcttgcagc tgaagataaa
     3721 cggatcctct atctgactaa ttgggtaatg tttgcatact atcttttaat gcaaacattg
     3781 acttatttag agtatgacat cggattgccg acaatggctt atcgtcaatt tattctggtg
     3841 acttatcttg ttatcttcat gggaattatc aaacaacttg attttcattt gcgacagaaa
     3901 ctacaagacc agcttgattt tcaacaagac ttacaactaa aaagcttgga agattacagc
     3961 aagcaggttg agaatcttta tcgtgaagtg aaagggttta ggcatgatta cgctaatctg
     4021 atgaccacat tacgcttggc tattgaagat aatgacattg agcaaataag ggacatctac
     4081 caaacagtat taaaagattc aagtaaagaa tttcgaacca gtaaatatga catcggacgc
     4141 ttggtcaata ttgataactc cgccttgaaa agtctattgg cctccaagtt cttacaggct
     4201 aatgataaca atgtatcagt tagcttggag gtgccagaag tgatcaaacc gcaaggaatg
     4261 gagttggtag attttatcac aatagtatcg atattatgtg ataacgccat cgatgctgcc
     4321 atagacgccg ccactccaaa ggtgacgata gcttttttaa cggtcaatga taagcagatg
     4381 tttattatcg aaaataacac caaggaagaa agagttgacc tttcagagct ttatgccttt
     4441 ggtcatagtt ctaaaggcga gggcagaggt attggtctat ataatgtcat gaaaattcta
     4501 gagcgttatc cactgatatc gctgaacacg actagtaagg ctcatctgtt tggccaatct
     4561 ctagaaattg acttgtcatc gaaataaaaa aagttggacc atgggattta atccctcggt
     4621 ccagattttt tgatgttaac ccagtttatg tatattgttg tcacctgcaa ttttatcgtg
     4681 tctaggaaaa aatctaatca gatcactcca ccagtcccca ccagtgatgc ggtggagttc
     4741 tgacgaggtt agttctgaga aatgcgtgag tgattttgtt tgcttggtca tagtaggacc
     4801 tcctttattt aatctttatt ttattgtaca caaaatatag caatttttga cattcaaatc
     4861 ctgaacagtc atttaacttt cctaaatggt taatcatggt tggataaaat tttatcctta
     4921 tagtaattaa agtaagtctt cttagcaata atactcgtga ctcggccctg gagaccgtat
     4981 tggatgatgt taccatgttt gtcggataat tgagccaatg cggtaacttt gaaaagattc
     5041 ccctcctcag ttttagtagc agttttatca atagacttaa tttttccaat aacagtgatg
     5101 ctttggttgc caactttttc taagctcagg cgtacgtctt gttcttcttt gagtaaggcg
     5161 acatgctctg aagtcacgta gtaagtgatg aggaattctt tggtttgatt gatatcgggg
     5221 taaatctgag cgatttcact accgcttgga atcaaagtct ttccttcaaa ctcactattg
     5281 agatggatta cccctgtttc aggagctgta atggtattgt tttctagtcg gacggatgct
     5341 tgctctagct gtgctgctag ctctgtgatt tgcgtttcaa gggtggtcag ctgctgagag
     5401 gccgattgta aaaactgtgt tcttaggacc tcaacctttg ttacaaagct gttatcatag
     5461 gttgcaacag agccagttcc agctcgttgg atgttcagac tagctataga tgattctagc
     5521 cctgagatac tttggttgat ttgagaaagg tattgatcag ttaatcctgt atgggagtct
     5581 tgttgggatt gggctaagaa attgtttaag gtggcttggt gtggattgcc agctgataaa
     5641 ccggtctgtc tattggtaat ggcttggcga agctcctcgt attctgctat ttggtgatag
     5701 atttgattga tttgcgtgtc aatcgcagca atggtattac ctgctagggc ggcctggtta
     5761 ctcacttctg cattgatttt ggaaattcct agttcgatgt cctgtgactg ggtgataaag
     5821 ttgttaaaag tattgatata gccaaattcg tcctcccctg taaagaggtt cgttccctgt
     5881 gccaggcttt ctttgagggt ttcaagtcca gttttctgtc tatttagagt ggctagctgg
     5941 gtttcaaggg cttgtttttg ggaactctcc atagtttcag cgtattggat gatgagctca
     6001 ttctttttaa ccagcttgtt attggctaga ttattggttg tgatagggtt attgctggtg
     6061 gattggatgg aagcgatgat tgtcgtcgga gtaatttcac ctcttgatgt gacggtaacc
     6121 tcctttttgg caaaaaggga gaaggttaga agaaagccga ctaataatat caaggggaca
     6181 attaagagtg tcgcaaaatt atggtagcgc cgctggtaaa attcagcact tttgaacaaa
     6241 ttagggttca tgcttagtct ccttacttgc taaatagatg atggtagaaa ccttgtttgg
     6301 tcatcaattc gtgatgtgaa ccagtctcaa tgaccttccc ttggtcaaga acgataactt
     6361 ggttggttcg ctcagaaata ctgaggcggt gtgcgacaaa gataatggtc ttgtctgcca
     6421 tggccattaa attatcaatg acctttttct ccgttagcac atcaaggccg ctagtggctt
     6481 catcaagaat aagaacaggg gctttagtca gtagagccct agccaaagca atccgttgct
     6541 tttgaccacc cgataagcca gctccgtcag acaattctgt ttgatagccc ataggcatct
     6601 gctcgatgtc ttgtcgaatt tcagcgatct cacaagctct gataatatct tcctgactaa
     6661 tcagttgatt ggcccctaaa gtcaagtttt ctaagattga accactgaag atataagctt
     6721 gttgtgggag gtagttaatg tgttgccgaa gcgtcttttt atcaattagt ttaaggtcat
     6781 tgttgttgat agtgattcga cccttgtaag gctcaaagaa attgacaatc atcttggcca
     6841 aggttgtctt tcctgagccg ctaattccca ctagactgac cttatcgcct tgtttgatgg
     6901 tcagattgat atccgataga gtgtcacgtc cgaaaccata cttataggaa aggtcttcaa
     6961 agcgaatatc gccagtcagg aactgtgaat ctgttaaggt ctgactgtta ctgaattcag
     7021 attcaaccag gtagacttca ttcaggcgat tgttagccac cttggcagac tggagcttgg
     7081 tttggaggtt tataatattt tctaaaggat tggtaaaata agatagaagg gtgttgaagg
     7141 tgatcagttg tccaactgaa atgtgtccgt tcatgactag ctgagcccca aaccacaaga
     7201 tgacaatatt tagaatgagc tgagctccct gtttaagggt agtttgcagg atagagtatt
     7261 tactgagttt gaaggatttg tccaggtaat ctacgaactc gctatcaatc ttctggtagc
     7321 ggctttcttc gctggtcaag gatttgatgg tttcaatgcc gttgatgtct tcgataatgg
     7381 cagagctgac catggaattg ctctgcataa catcgttatt catcttctca aacagcttca
     7441 taaaagcaaa aataatgatg atgtaaatgg gaactgaaat taaggagagc aggaagaggt
     7501 tggtgttttg taccaaaagc acaccaccga cgatggtcaa aatagagaca tcaaggaaga
     7561 atgacaaaat ggtcgatgcc agggcatcaa tgattgaatt ggcatctgta aagcgagaga
     7621 taacctcacc agtccgccgc gtcgcaaaga aggacatggg cagttcaaag atatgacgga
     7681 tataggaaag aatcacatca atacttagcc gctggctgag cacagtcaaa aggtaatctc
     7741 gtgagaaact catcatctgc tggaggatgt aggtgataat caggccgatg gagataatcc
     7801 ccaaggttga cttcatttga ttaggaatgt attcatctaa aatcccttgc aggtagtaag
     7861 aaccaccaat attgataagc gtcaccaaaa ggctagccaa gacgatataa aagataagcg
     7921 cacgttgcct gaaaataaga gggagaaagc tcatcaggcc gttcttttta tctttgtgag
     7981 gcttatagct gggttctgga gccagaaaga tagcgacgcc tgtccattca gaagcaaacc
     8041 gctccttggt catcttggtg actttgaccg tagggtcagg atcaccaatg atgaggtggt
     8101 cttttttgtt gtgatagacc acatagtagt gttgcagttt gccttcttta ttgacgtgaa
     8161 cgatgaaggg atagggaacg tcatccatat caaagaggct catgtcagcc tggatggctc
     8221 tagtttcaaa gcccatctct ttggctgctt tgacgatacc aagagcggtc gttccttcct
     8281 tattggtttt ggccaattct ctcaaatgag ctagggaata atcagaaccg taaaacttgg
     8341 caatagaggc taaagcagcc acaccacagt ctctggcatc gatttgagga acaaaagttc
     8401 gtttgtaagc tgtcatttgt caaatccttt cttgttagtg acaagactat tatgacaaaa
     8461 aaatcacaga aaaaaatatc caaatcttaa atggtcatcg aaacgtcctg aatggtaaaa
     8521 agatatttcg gctcttccta aacaatacat acctgaacgg tcgctttccc ttcttgaatg
     8581 gtaaaatttt cccatttagg aaagttaaat gactgttcag gaaatgggga aattattttt
     8641 tgaagaagtg ctatactaga cttgtcaagg ttgcaacccg acaaagaatt tgtatctgga
     8701 ggctgacaat gttaaagagt aaaatttact atattgcaaa tattctttat ggaatattaa
     8761 ttttttcgct tggtttagaa tcaaaggtag ttattcttac tagtttaatg tcaatagtta
     8821 tagtagtttt ttcgataata ggacttataa aatcaaaaca aaaggagtag atatgttaga
     8881 attaaatgtc gaaaaattag agacgatttc tggaggcgga aatgtgaatg actgtgtgaa
     8941 tggtattctt gcctctgctg gggtcttggc aattgctgct acaggcccta cagggtggtt
     9001 agcacttgct gctcttggtg gcgcagctgg taccgctggt tttgccactg gatggtcatg
     9061 tactgcactt tattacaatg gagactagga ggaacaatga tgatagcaga aattaaagat
     9121 gatattttag aaaaagtttc aggtggagga aatgtatcag attgttttct gtccgcaagt
     9181 ggtgcactag gcacaattgc tggagctgga gcagtaacag gtcctattgg ttggggtgtt
     9241 gctttagtgt cagcagctgg ttttggattt ctaacaggat ataattgtac taagttatac
     9301 tttgaaggag attaaatttg gacaataatg aaaatttttt agatagctaa aagatattca
     9361 ttattgacaa ataactatac ttcagataca ggtgtgattt taaagactgg gcaaaaatgt
     9421 ccagtctttt ttgtatcgct caaacttatt aaggattatt catctgtatg gtcaacttat
     9481 ataaaagatc actttacctt cttgagtggt aaaattttcc catttaggaa agttaaatga
     9541 ctgttcagga aatggggaaa ttattttttg aggaagtgct atactagact tgtcaaggtt
     9601 gcaacccgac aaaatataaa atctatatca taaaggaggt attcatcatg aatacaaaaa
     9661 caatggaaca gtttaacatg ctaaatgctg atactcttgc tactattgag ggtggtggta
     9721 ttttcatagg ttttcgatgg gtatgtaccg atggtaaaaa gagtgcgtgg cacatgttta
     9781 aaggtacagc tcaggataat gcaaataatt atatgctcag aaatccagga gttacttgca
     9841 cagttgacca tgcctaatct ataaatagtc acccttacgt tttaacctaa aacgtaagcg
     9901 taactatttc ttataaaagg agtttctaat gttactggaa ttaaagcaat tagctaagac
     9961 atatagtaca aaaatagttc tcaatcaaat ttcggttcag ctgaccccag gcctctatgg
    10021 tttgctgggg gcaaatggga cgggcaagtc aaccctgctt aacctgatct gccattttac
    10081 caaacccgat agcggaacca tagagctgga cggtaagcgt gaggctagct acttttatga
    10141 gcaggtaggt tttctgcccc agcatttcag ctattacggc aactttacag gcctggattt
    10201 cctgctctac atggcaacct taaaaggcat gtcaaaaaaa aggcccaaaa agaagctgac
    10261 cacctgcttc ggatggtggg cctgcacgat gttcgtcgta agcggattgc cacctactct
    10321 gggggcatga agcagcggct aggcatcgcc caagccctgc tcaatgaccc gaaaatcctg
    10381 attttggacg agccgacagt aggtctagac cccaaggagc gggtcaagtt ccgcaacatc
    10441 atcagcgacc tgtccgctcg caagattatc ctcttgtcca cccacattgt ctccgacgtg
    10501 gaggccatcg ctaaggagat tatcattttg aaaaatgggc aatttcttca gcagggcagt
    10561 agccaagagc tgcttgagac catgaacggt aaggtctggg agatccccgt gcctgacggt
    10621 tcacacctgc caaccggtag ggactttgcc atagtcaatg agaaagtgac ctcatctggc
    10681 cgtgtgtttc gtgtggtcag cgattaccaa ccaatcccgc aagctcaacc tgttcagccg
    10741 aatttggaag acctctacat ttaccacttt agagaggagc aagtctcatg agaaccatct
    10801 tatcctttga attgactaaa ttactctgta aaaaatcagc tctaggagca gtaacagttg
    10861 ccctactagc cttgcttggc ttgttttata ctaatttttt tgtcggtcag attagcgggt
    10921 attcttctga caaagttaat ggcaaagcag ctgtagccat caatcggcag attgctgaag
    10981 aacacacggg ttatttatca gatgatttga tgagccgcat tctcaatgac tacgctaaaa
    11041 accagcctga cctcaagaaa aaaggggttt acagcgtggt ctctcattac gctattagtg
    11101 agttaatccc tgattctaat gagatgttaa ttgctattaa ccagaccaat gaggtgctcc
    11161 agttcgacaa cattgaactg aaatctcagg agcagttagg ttctcatttt ccacttgagg
    11221 agttgaaatt agggaatttc gccccttgga accagctttt tctggtcagc aacagttcct
    11281 atattctcat tttgctactg tctgtttacc tgtcagctcc cttgttttct ggtgatttta
    11341 gtaagaagat gaacccactt ctcttgacga ccaaatacgg tcgaagcaaa ctaacggttg
    11401 ctaagttgac agcaagttta ctgattagca ctagcttgtt tgttgttttt aacgccatca
    11461 ttttagctgt ctttgcctgg tactttggtt ttagtggttg ggacactagc gtccaactta
    11521 acctttattg gattgttcct tttgaaaata ttatggcctt tccagagaaa ttgacactcc
    11581 tgtcaacctt tgtcaaccta attgtctttc agtttatcgg cttggtcttt atcacggcag
    11641 taaatgccct tgtttctagc aaaaccaagt ctcccctgac cacttttgct gtgtccgctg
    11701 gtacaatttt tgtgcccagc ttcctcttgc aggtcttcca aggtggtctt atccacaaac
    11761 tgctgaccat catctctgta ccaacgtcaa acaccgcagg cttaatgttg caactatctc
    11821 atcagggacc acaaggattt ttcttagatc atttttggac aaatggtggc ctgataattg
    11881 gatttagact acttattgtt gggttgtttt tttatcgtac atttaagata atcacgaaag
    11941 tacagcgcta atgattggaa taaattattc atagaggaac cttttgacaa taagtaaagt
    12001 caaaaggttt tatttttttg tatagagatt tttagttatg attcataatt tcagtttatt
    12061 tgaacttttc tggtgttgta tgaggtagac tgaaaagtaa aagatttttt cgacttgctt
    12121 aaaaaaataa tacctaagcg ctcactttac tttcttaaat ggtcaaagtt tcccatttag
    12181 gaaagttaaa tgactgttca ggaaatggag cattattttt ccaaaaatca gctatactaa
    12241 acttttcaaa gttgcaaccc gacaaaatat aaatctacat cataaaggag gtattcgaga
    12301 tgaatacaaa gaaaatggaa cgatttaaca cacttaactc tgaggccctt gcaactgttc
    12361 aaggtggtga gggaggatag tttctcccat tgttaccaac ttataaagaa attttatcta
    12421 gtatttgcgg tcttgcagat gggttgaaag gtagaaagca taaaggaagt tgctagaagg
    12481 tagctagcta caagtaatag ataggaaaag caaaatgagg ttactaaaac tatgtggttt
    12541 gattgtgtta acaatcttct taagcttagt ggtacaaatt ccaatttatt tagggattaa
    12601 tatgtttaaa gggagtgttg ttcttaaaat aacgattttg ttaatagctt tcttgctgtt
    12661 attgggatgc ttatcactgg tcaaagtcaa gtgttttcct aatatccctg ttaaaaaata
    12721 tctctctttg agaacgactg taggttggac gctattatca ttcttgttaa ttattggg
//
LOCUS       DATUCG010000066        12766 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.067, whole genome shotgun sequence.
ACCESSION   DATUCG010000066 DATUCG010000000
VERSION     DATUCG010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12766
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.067"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            <1..1714
                     /locus_tag="VCA20_001454"
     CDS             <1..1714
                     /locus_tag="VCA20_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEP1835262.1"
                     /translation="YPEVQQMIEDTYNSLYNRTVSKTYDCSHLTIDGLAQNISLRPHQ
                     KNAIQRIVEEKRALLAHEVGSGKTLTMLGAGFKLKELGMVHKPLYVVPSSLTAQFGQE
                     IMKFFPTKKVYVTTKKDFAKAKRKQFVSRIITGDYDAIVIGDSQFEKIPMSREKQVTY
                     INDKLEQLREIKLGSDSDYTVKEAERSIKGLEHQLEELQKLERDTFIEFENLGVDFLF
                     VDEAHHFKNIRPITGLGNVAGITNTTSKKNVDMEMKVRQVQAEHGDRNVVFATGTPVS
                     NSISELFTMMNYIQPDVLERYQVSNFDSWVGAFGNIENSMELAPTGDKYQPKKRFKKF
                     VNLPELMRIYKETADIQTSDMLDLPVPEAKVIAVESELTEAQKYYLEELVDRSDAIKS
                     GSVDPSVDNMLKITGEARKLAIDMRLIDPAYTLSDNQKILQVVDNVERIYREGENEKA
                     TQMIFSDIGTPKSKEEGFDVYNELKDLLVDRGIPKEEIAFVHDANTDEKKNSLSRKVN
                     SGEVRILMASTEKGGTGLNVQSRMKAVHHLDVPWRPSDVGRILRTFKIKKNVEVTDNG
                     KDNF"
     gene            1695..2069
                     /locus_tag="VCA20_001455"
     CDS             1695..2069
                     /locus_tag="VCA20_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001129922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnpV protein"
                     /protein_id="HEP1835263.1"
                     /translation="MAKTIFEEMGGKYERQGDYLIPCLTVPAEEEQPIGIWGQRHLDY
                     LKHHCKVTYTNLLTSGRLNAYLADIDRQAQERFERLIEGMKQAQGITEQLKAENALEW
                     TGCLNNIRACAREIVEKEIIFA"
     gene            2436..4355
                     /gene="tet(O)"
                     /locus_tag="VCA20_001456"
     CDS             2436..4355
                     /gene="tet(O)"
                     /locus_tag="VCA20_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032490535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(O)"
                     /protein_id="HEP1835264.1"
                     /translation="MKIINLGILAHVDAGKTTLTESLLYTSGAIAEPGSVDKGTTRTD
                     TMNLERQRGITIQTAVTSFQWEDVKVNIIDTPGHMDFLAEVYRSLSVLDGAVLLVSAK
                     DGIQAQTRILFHALQTMKIPTIFFINKIDQEGIDLPMVYQEMKAKLSSEIIVKQKVGQ
                     HPHINVTDNDDMEQWDAVIMGNDELLEKYMSGKPFKMSELEQEENRRFQNGTLFPVYH
                     GSAKNNLGIRQLIEVIASKFYSSTPEGQSELCGQVFKIEYSEKRRRFVYVRIYSGTLH
                     LRDVIKISEKEKIKITEMCVPTNGELYSSDTACSGDIVILPNDVLQLNSILGNEMLLP
                     QRKFIENPLPMLQTTIAVKKSEQREILLGALTEISDGDPLLKYYVDTTTHEIILSFLG
                     NVQMEVICAILEEKYHVEAEIKEPTVIYMERPLRKAEYTIHIEVPPNPFWASVGLSIE
                     PLPIGSGVQYESRVSLGYLNQSFQNAVMEGVLYGCEQGLYGWKVTDCKICFEYGLYYS
                     PVSTPADFRLLSPIVLEQALKKAGTELLEPYLHFEIYAPQEYLSRAYHDAPRYCADIV
                     STQVKNDEVILKGEIPARCIQEYRNDLTYFTNGQGVCLTELKGYQPAIGKFICQPRRP
                     NSRIDKVRHMFHKLA"
     gene            4407..4580
                     /locus_tag="VCA20_001457"
     CDS             4407..4580
                     /locus_tag="VCA20_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002779755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEP1835265.1"
                     /translation="MMKCEWILCPVCGSKTRNKIRKDTVLENYPLYCPKCRQESLIKV
                     DNLKITVIKEPDA"
     gene            5070..5579
                     /locus_tag="VCA20_001458"
     CDS             5070..5579
                     /locus_tag="VCA20_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEP1835266.1"
                     /translation="MKYAPRKVYIKESGGYVELSYTDFCRRRQADKGYMDKLFIPVQG
                     CLLEVVREQYADFYRDRERWRYLQKLDARNSLLSLDGFTDSEGNPLDFIADEAADIAE
                     TVVNAVMVDRLKAALPLLSDSEQELIQAIFFDGLSEREVGARLGITQSVVNKRKARIL
                     RKLRKIIEN"
     gene            5674..6099
                     /locus_tag="VCA20_001459"
     CDS             5674..6099
                     /locus_tag="VCA20_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEP1835267.1"
                     /translation="MAYNHGREDRKWRIWKEAEEKLLRECGVDEVTIEQIRIADRADF
                     NSNRRFYRWTNDVAEYLEDMADRERQAEVNTVAELLDEIESENLYQVLVTVDGRTLKI
                     VLLKMQGYSTKEIAPLVHLTTGAIYARLDHLRKKLRKIL"
     gene            6183..6656
                     /locus_tag="VCA20_001460"
     CDS             6183..6656
                     /locus_tag="VCA20_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEP1835268.1"
                     /translation="MAYKAKAYTLREESTESGIRYFISFKDGQGEHHELEVSEQLFFE
                     FRQMERRNRNLLQWDERHREFSEVWDETLNRRALKLPKSIEEQMIEAERAELLCKAVD
                     RLPEIQRRRFLLYYEYEFNFYQIAAMEHCTASAIQKSVAVAKKKVKAEMRKYLQP"
     gene            6810..7841
                     /locus_tag="VCA20_001461"
     CDS             6810..7841
                     /locus_tag="VCA20_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002596255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEP1835269.1"
                     /translation="MQKLQTVNAETLLYEPLEKPSFVVDSLIPTGLSLFCGSQKIGKS
                     WLMLKLCLCVSQGIPLWDMTTMEGDVLYLCLEDTFCRIQDRLFRLTDEASGRLHFAVA
                     SCKLSDGLIVQLEDYLKDYPDSRLIVIDTLQKVRTASKDNAYASDYGDISLIKDFADR
                     HSLAVIVVHHIRKQNDSDVFNKVSGTTGLTGSADATFVLEKEKRASDTAKLYVTGRDT
                     PYQEYTLRFRDCSWELVERKTQEQLAKETIPDVLFRLVDFMRDKEEWAGTATELLAAM
                     GETETIPTVITKWLNEYHTTFLSENRICYQYSRRKDGRQIALARRAGDSGDGGDSDIG
                     IPPVTVIDA"
     gene            8087..8353
                     /locus_tag="VCA20_001462"
     CDS             8087..8353
                     /locus_tag="VCA20_001462"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835270.1"
                     /translation="MPDVPSGLPYGRSYALLSYALRLRIKERRHDGYSSCEGVSQKHR
                     HHRHDRHAGDKGDSYGNRQGASQNCRHHRHRCHPKGSYPPKKGR"
     gene            8360..9325
                     /locus_tag="VCA20_001463"
     CDS             8360..9325
                     /locus_tag="VCA20_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HEP1835271.1"
                     /translation="MPYAILRFQKRKAGGVAACERHNERKKEAYKSNPDIDMERSKNN
                     YHLVAPPKYTYKKEINRKVAEAGCRTRKDSVMMVETLITASPEFMNQLPPEEQKAYFT
                     MALDFISERVGEQNILSAVVHMDERTPHMHLCFVPITPDNKLSAKTILGNQKSLSEWQ
                     TAYHERMSSRWNQLERGQSSMETKRKHVPTWLYKLGGRLDKQYGEIVSALSDINAFNA
                     GKKRDKALELVAAWLPEVEKFSKEIGRQQAYIDSLKEQIGQEADYAGRMRDEKYEQEL
                     KVQKANQRIFELQRTNEQMGRLLSKIPPEVLEELQRTGRNKSRER"
     gene            9333..10187
                     /locus_tag="VCA20_001464"
     CDS             9333..10187
                     /locus_tag="VCA20_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002596257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEP1835272.1"
                     /translation="MKKQDFKVLKTKDLYPFPDNPFHVVEDETLSELAESIKEFGIVT
                     PIITRPKEDGNGYEVIAGQRRVRASELAGINTVPAFVLPLDRDRAIITLVDSNLQREN
                     ILPSERAFAYRMKSEAMKRQGFRTDLTSSQVVTKLRTDDKVAQGFGVGRMTVQRFIRL
                     TELIPPILQMVDEGKIALTPAVELSFLKKDEQENLFATMESEEATPSLSQAQRMKSMS
                     QSGQLDMDMIFSIMTEEKGNQKETLKINTSKLKKYFPKDTTPKQMEETIIRLLERELQ
                     RKRSRDSR"
     gene            10264..10494
                     /locus_tag="VCA20_001465"
     CDS             10264..10494
                     /locus_tag="VCA20_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835273.1"
                     /translation="MKEIQYEIVKEIAVLSASDSGYTKEINLISWNGREPKYDIRSFS
                     PNREKCGKGITLNADEAAALLEALQKEVNSGD"
     gene            10565..10756
                     /locus_tag="VCA20_001466"
     CDS             10565..10756
                     /locus_tag="VCA20_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HEP1835274.1"
                     /translation="MGEDRKTTGRRKRIVPKGQVQMIISREYIGTQTVAEAFVPIISE
                     DIRKKIAEGDTFDNEGLSA"
     gene            10810..12693
                     /locus_tag="VCA20_001467"
     CDS             10810..12693
                     /locus_tag="VCA20_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEP1835275.1"
                     /translation="MAGIRTENKIYEVGIYCRLSKDDGTDNESASIATQKSILTDYVK
                     RQGWHLAKTYVDDGYSGTNFQRPSFQNMIKDIENGLINCVITKDLSRLGRNYLDCGLY
                     LEVFFPEHNVRYIAVNDGVDTLNKSAMDITPFRNILNEMYSADVSVKIKSTYRARFQQ
                     GKFMGTTAPYGYVKDPADHNHLLIDDKVAHVVREIFDLALAGNGIAKIRKHINKQHIL
                     RPAAYAVEQGATGYERYFEDNEENRYIWSENSVRGILRSPIYAGNLAGYKRIAANMKS
                     KKRPSKLPEEWEVIPDTHEGIVTQEEFDTVQQLMTSRRREQNAGGFENIFSGVIKCAD
                     CGYAMRAASANRRKRPDIIDCVQYTCNNYGRYGNVMCTAHSIEARDLFNAVLADINRF
                     ADMAVNDEKAVRAIERRLTETDQSRAKSLEKEKKKLNKRLAELDRLFSSLYEDKVMER
                     ITERNFEMMSGKYQKEQLEIEARLKEVTETLNDSYEKSQGVRDFLSLIRNYQGLKELD
                     ATIINALIDKILVSEREKLADGTVRQEIKIYYKFIGFVGELHITPTKRWTALKPKNCT
                     VCGVEYVPSSGISKYCPACAKRIQREKSNESKRRSRERNRRACIELSAKNDRLILSSE
                     MDD"
BASE COUNT         3904 a         2580 c         3286 g         2996 t
ORIGIN      
        1 gtatccagaa gtccaacaga tgattgaaga cacctataac agtctctata atcgtacagt
       61 atcaaagacc tatgattgta gtcatttaac cattgatgga cttgcccaga atatctcctt
      121 acgtcctcac caaaagaatg ccattcaacg aattgtggag gaaaaacgtg ccctactagc
      181 tcatgaagtt ggttcaggta aaacacttac catgcttggg gcaggattca aactgaaaga
      241 actcggaatg gtacataaac cactttatgt ggtcccttct agtttgactg cccagtttgg
      301 tcaagaaatc atgaaatttt tccctaccaa gaaagtctat gtgactacta agaaagactt
      361 tgccaaagcc aaacgtaagc agtttgtgtc tcggattatt acaggggact atgatgccat
      421 tgtcattggg gattcacaat ttgagaagat accgatgagt cgtgaaaaac aggtgaccta
      481 tatcaatgac aaactcgagc aactccgaga aatcaagcta ggaagtgaca gtgattatac
      541 ggtgaaagaa gcggaacgtt cgattaaggg attagaacac cagttggaag aactccaaaa
      601 actagagcga gatactttta ttgagtttga aaaccttggc gttgattttc tttttgtgga
      661 tgaggctcat cactttaaga atatccgtcc aatcactgga cttgggaatg tagctggaat
      721 caccaacaca acttctaaaa agaacgtgga tatggagatg aaggtgagac aggttcaggc
      781 agagcatgga gatagaaatg tcgtgtttgc gacaggaacg ccagtctcaa actccattag
      841 tgaacttttc accatgatga attacattca acctgatgtt ttagagcgat accaggtatc
      901 caattttgat tcatgggtag gggcttttgg gaatatcgaa aattctatgg aactagcacc
      961 gacaggggat aaataccaac ccaagaaacg ctttaagaag tttgtgaacc tgcctgaact
     1021 catgcggatt tataaggaaa cagctgatat tcagacctca gatatgcttg atttacctgt
     1081 tcctgaagct aaagtcattg cggtagagag tgaactcaca gaagctcaga aatactacct
     1141 tgaagagttg gttgaccgtt cagacgctat caagtcaggc agcgttgacc ctagtgtcga
     1201 taatatgtta aagataactg gggaagctag aaaattagcc attgatatgc gcctcattga
     1261 ccctgcctat actttatctg acaaccagaa aatccttcaa gtagtggata atgtggaacg
     1321 catttaccgt gagggagaaa atgagaaagc tactcagatg attttctctg atattggaac
     1381 ccctaaaagt aaggaagaag ggtttgatgt ttacaatgaa cttaaggact tgttggtcga
     1441 tcgaggaatt ccaaaagaag aaattgcctt tgtccatgac gccaatactg atgaaaagaa
     1501 aaactcattg tctagaaagg tcaatagcgg agaagtgcgg attctcatgg cttcgacgga
     1561 aaaaggtgga acgggtttaa acgtgcaatc acgcatgaaa gctgtccacc acttagatgt
     1621 tccctggcgt ccctctgatg taggacggat tttgcggaca ttcaaaataa aaaagaatgt
     1681 ggaggtaaca gacaatggca aagacaattt ttgaggaaat gggcggcaaa tacgaaaggc
     1741 aaggcgatta tttaataccg tgcttaactg tacccgccga agaagaacag ccgataggca
     1801 tctggggaca gcggcatttg gattatctga agcatcactg caaagttaca tacaccaatc
     1861 ttcttacaag cggcagactt aacgcttacc ttgccgacat tgacagacag gcacaggaac
     1921 gctttgaaag gctcatagag ggtatgaaac aggcacaggg cataacggaa cagctaaagg
     1981 cagaaaacgc cttagaatgg acaggatgcc tcaataacat aagggcttgt gcgagggaga
     2041 ttgtggaaaa ggaaattatt tttgcataaa cagatgatta gtggcagggg gaaatcctgc
     2101 cgctttttct gctttagttt gtcagcttga caaataaagg gttaaggaat ataattagat
     2161 tcagtattat acaaggagtt aataaatatg cggcaaggta ttcttaaata aactgtcaat
     2221 ttgatagtgg gaacaaaaag tagcagtccc gtttcacttt taatatgggg cttagttttt
     2281 tgtacccagt ttaagaatac ttttatcatg taattttata tgcccgaaaa catataagtg
     2341 ttttggggct attggagtta tttacccagt gataggagta tttatcactg ggtattttta
     2401 tgcccttttt tgggtgttga taggaggaaa atcacatgaa aataattaac ttaggcattc
     2461 tggctcacgt tgacgcagga aagacaacat taacggagag tttattgtat accagtggtg
     2521 caattgcaga accagggagc gtagataaag gcacaacaag gacagataca atgaatttgg
     2581 agcgtcaaag gggaatcact atccagacag cagtgacatc ttttcagtgg gaggatgtaa
     2641 aagtcaacat tatagatacg ccaggccata tggatttttt ggcggaagta taccgttctt
     2701 tatccgtatt agacggagca gtattattag tttctgcaaa ggatggcata caggcacaga
     2761 cccgtatact gtttcatgca ctacagacaa tgaagattcc gacaattttt ttcatcaata
     2821 aaattgacca agaggggatt gatttgccaa tggtatatca agaaatgaaa gcaaagcttt
     2881 cttcggaaat tatagtgaag caaaaggttg ggcagcatcc ccatataaat gtaacggaca
     2941 atgacgatat ggaacagtgg gatgcggtaa ttatgggaaa cgatgaacta ttagagaaat
     3001 atatgtcagg gaaaccgttt aaaatgtcag aactggaaca ggaagaaaac aggagattcc
     3061 aaaacggaac gttatttccc gtttatcacg gaagtgctaa aaacaatctg gggattcggc
     3121 agcttataga agtgattgcc agtaagtttt attcatcaac gcctgaaggt caatctgaac
     3181 tatgcgggca ggtttttaag attgaatatt cagagaaaag gcggcgtttt gtttatgtgc
     3241 gtatatatag cggaacattg catttgaggg atgttattaa aatatctgaa aaagagaaaa
     3301 taaaaatcac agagatgtgt gttccgacaa acggtgaatt atattcatcc gatacagcct
     3361 gctctggtga tattgtaatt ttaccaaatg atgttttgca gctaaacagt attttgggga
     3421 acgaaatgct gttgccgcag agaaaattta ttgaaaatcc tctccctatg ctccaaacaa
     3481 cgattgcagt aaagaaatct gaacagcggg aaatattgct tggggcactt acagaaattt
     3541 cagatggcga ccctctttta aaatattatg tggatactac aacgcatgag attatacttt
     3601 cttttttggg gaatgtgcag atggaagtca tttgtgccat ccttgaggaa aaataccatg
     3661 tggaggcaga aataaaagag cctactgtta tatatatgga aagaccgctt agaaaagcag
     3721 aatataccat ccacatagaa gtcccgccaa atcctttctg ggcttctgtc gggttgtcca
     3781 tagagccgct ccctattgga agcggagtgc agtatgaaag cagagtttca cttggatatt
     3841 taaaccaatc gttccaaaat gcggttatgg agggggttct ttatggctgc gagcaggggc
     3901 tgtatggatg gaaagtgaca gactgtaaaa tctgttttga atatggattg tattatagtc
     3961 ctgtaagtac ccccgcagac tttcggctgc tttcccctat cgtattggag caggctttaa
     4021 aaaaagcagg gacagaacta ttagagccat atctccactt tgaaatttat gcaccgcagg
     4081 aatatctctc acgggcgtat catgatgccc caaggtattg tgcagatatt gtaagtactc
     4141 aggtaaagaa tgacgaggtc attctgaaag gagaaatccc tgccagatgt attcaagaat
     4201 acaggaacga tttaacttat ttcacaaatg ggcagggagt ctgcttgaca gagttaaaag
     4261 gataccagcc agctattggt aaatttattt gccaaccccg ccgcccgaat agccgtatag
     4321 ataaggttcg gcatatgttc cacaagttag cttaacagct tgcaaaagtc atataaaatg
     4381 agatttgaaa ggattagaga ctaattatga tgaaatgcga atggatattg tgtcctgttt
     4441 gtgggagcaa aacccgtaat aaaattagga aggacactgt tttggagaat tatccccttt
     4501 attgtccaaa atgcagacaa gaaagcttga ttaaagttga caacttgaag ataactgtca
     4561 tcaaagagcc agacgcttaa gacgcagagc cgatgaaatt gtggaacaat tcacgaatca
     4621 tcggctcttt ttgtttcgta tttgaaagaa caagctcacc aaataaaaaa aacgatattc
     4681 gggtgggtta ttttgttata ccctaaatta ccctctgaat ttgtttttaa atttggaggg
     4741 atttttttat gttctttttt cgggcggtta ttcatctgct catacgaagt aaaatttatc
     4801 aatacatagc atatcagaga atggcaggaa accagttaaa aaaatttctg ccagtgcagc
     4861 ggtacttctc accttgaaag tggaagtaca atctccatac aacagaattt gtatttccca
     4921 taaccgtgaa ggtcacagag cctttgcggt ttttcttttg tcgttttttg ttggaaagtg
     4981 ccgcagggct gtttctggtg tgcgttgccg ctccccgcct ctccatctgg atttttacat
     5041 ttcaaaaatt cagattggag gtattagcgg tgaaatacgc accaagaaaa gtatatatca
     5101 aagaaagcgg cggctatgtg gaactgtcct atacggattt ctgccgccgc aggcaagccg
     5161 acaagggata tatggacaag ctgtttatcc ccgtccaagg ttgtctgctt gaagtcgtaa
     5221 gggagcaata tgcggacttc taccgtgaca gggagcggtg gcgttacctg caaaaattag
     5281 atgcgaggaa cagcctgcta tctctggacg gatttacgga cagcgagggg aatcctctgg
     5341 actttatcgc tgatgaagcg gcggacattg cggaaaccgt tgtcaatgct gttatggtgg
     5401 acaggctgaa agccgccctg cctttgttgt cggatagtga gcaggagttg atacaggcaa
     5461 tcttttttga cggactttcc gagcgtgaag tcggggcgag gttaggcata acccagagcg
     5521 ttgtgaacaa acgcaaagcc agaatcctaa gaaaactaag aaagataata gaaaattaaa
     5581 atttaaggcg ttcagccccc ttgttttttc ctttgggaaa atgagggggc ttttctctgc
     5641 cctctcaatc aattctgatt ggaggaagaa agaatggcat acaaccacgg acgggaggac
     5701 aggaaatggc gtatctggaa agaagcggag gaaaagctgc tgcgtgagtg cggcgttgat
     5761 gaagtgacca ttgagcagat acgcatagcg gacagggcag acttcaattc caacaggcgg
     5821 ttttaccgat ggacgaatga cgttgcggaa taccttgagg acatggcaga cagggagcgg
     5881 caagcggagg tgaatacagt tgcggagtta ctggacgaga ttgagagcga aaatctctat
     5941 caagtattag tcacggtgga cgggcgtacc ttgaaaatcg tcctgctgaa aatgcagggg
     6001 tattccacaa aggagattgc cccacttgtg catttgacga ctggtgccat ctatgcgagg
     6061 ttagaccatc tgcggaagaa gcttcggaaa attttgtaag cgtctaatac atcccattat
     6121 tctgcgggct actgggtggg gagtggaatc tccccgctta tttttggcag gaggaaaacg
     6181 ggatggcata taaagctaag gcgtacacgc ttcgggagga atccacggaa agcggtataa
     6241 ggtattttat cagttttaag gacgggcagg gggaacacca cgaactggaa gtatccgagc
     6301 agttattctt tgaatttcgg cagatggaac gcaggaacag gaatcttctc caatgggacg
     6361 agcggcacag ggagttttcg gaagtatggg acgaaacgct gaacaggcgg gcgttaaagc
     6421 tgcctaagag cattgaggaa caaatgattg aagcagaacg ggcggaactg ctctgtaagg
     6481 cggttgacag actgccagag atacaaaggc gacgtttcct gctctactac gagtatgagt
     6541 tcaattttta ccaaatcgcc gctatggagc attgcaccgc ttctgcaata cagaaatccg
     6601 ttgctgttgc aaagaagaaa gtaaaagcgg aaatgaggaa atatctccaa ccatgaccga
     6661 caccgcccga aaaagaaatc tgttttttat ttgtgtggga ttggcggtat tacatactct
     6721 cacttttttc gtgggagtaa atctattttt aaggaggtca acgcctatgt gcaacaaaaa
     6781 caagataact gcccgaaagg aggaaaccca tgcagaagtt acagacagtc aacgccgaaa
     6841 cgctccttta tgagccgctt gagaagccgt cctttgtggt ggacagcctt atccccacag
     6901 gcttgtcgct gttctgcggc tcacagaaga taggcaagag ctggctcatg ctgaagctgt
     6961 gcttatgcgt gtcgcaggga atccccttat gggacatgac gacaatggag ggcgatgtgc
     7021 tttacctctg ccttgaggac acgttctgcc gcatacagga caggctgttc cgtttgacgg
     7081 acgaagcgag cgggcggctt cattttgccg tggcaagctg caagctgtca gacggtctta
     7141 tcgtgcagct tgaggattat ctgaaagact atccagacag caggctcatt gtcattgata
     7201 ccttgcagaa agtccgtaca gcttcaaaag acaacgccta tgcaagcgac tatggggaca
     7261 tctccctaat caaagatttt gccgacaggc actctctggc ggtcattgtc gtacaccaca
     7321 tccgaaagca gaatgacagc gatgtgttca acaaggtgtc tgggacgaca ggcttaacgg
     7381 ggagtgcgga cgctaccttt gttctggaaa aggagaaacg ggcatctgac accgccaagc
     7441 tgtatgtaac gggcagggac acgccctatc aggaatacac gctccgtttc cgtgattgca
     7501 gttgggaact tgtggagagg aaaacgcagg agcagcttgc gaaagaaacg ataccagatg
     7561 tcctttttcg gttggtggat tttatgaggg ataaggaaga atgggcaggc acggcaacgg
     7621 agctgttagc cgctatgggg gaaacggaaa ccatacccac ggtgattacg aaatggctga
     7681 atgaatatca caccacattt ttaagcgaga accgtatctg ctaccagtac agccgcagga
     7741 aagacggcag gcagattgcc cttgcaagga gggcgggtga cagcggtgac ggcggtgaca
     7801 gcgatattgg gataccccct gttactgtca ttgacgctta aagccatgta aagttggcgg
     7861 ctctgccctg cgggtgacag cggtgacggc agtgacagtg attttaggat accctgccgc
     7921 tgtcatccct acgggagaac accccgcaaa cgcaaaatgc aggcgtggga ttcacccgcc
     7981 cttttcgggc gtgtaaaatc acccctgcgg tcagaaaaat ctctccgatt tttccgactg
     8041 cgtttacagg gtgtaacaca ctacactttg ccctgcaaag agccgtgtgc cagacgttcc
     8101 ctctggactc ccttatggca ggtcttacgc cctgctatcc tacgccttgc ggcttcggat
     8161 aaaagaacgg cggcatgacg gttacagctc ttgcgaggga gtatcccaaa aacaccgtca
     8221 ccatcgtcat gaccgtcatg caggggataa gggtgacagt tacggcaatc ggcagggggc
     8281 atcccaaaac tgccgtcacc accgtcaccg ctgtcaccca aagggcagtt acccgccgaa
     8341 gaaaggaaga tgatggatta tgccctatgc aatcctgcgt ttccagaaac gcaaagcggg
     8401 cggcgttgcg gcttgcgaac gccacaacga gcggaagaaa gaagcctaca aaagcaaccc
     8461 agatatcgac atggaacgct ctaaaaataa ctatcatctt gtcgcaccgc caaagtacac
     8521 ctataagaaa gagattaacc gaaaggtagc cgaagcaggg tgcaggacaa ggaaagacag
     8581 cgtgatgatg gtggaaacgc tcatcacggc ttcaccagaa tttatgaacc agttaccgcc
     8641 cgaagaacag aaagcgtatt ttacgatggc tctggatttc atttcggagc gtgtcgggga
     8701 gcagaatatc ctctccgcag tcgtccacat ggacgagaga acgccccata tgcacctctg
     8761 ctttgtgccg attacaccag acaataagct gtccgccaaa acaattttag gcaatcaaaa
     8821 gagcctgtcg gagtggcaga ccgcctacca tgagcggatg tcctcacgtt ggaatcagct
     8881 tgagagaggt caatcttcga tggaaaccaa gcggaaacac gtccccacat ggctctataa
     8941 gttgggcggc aggcttgata agcagtatgg ggaaatcgtg tctgccctgt ccgacatcaa
     9001 cgcctttaac gcagggaaaa agcgtgacaa ggctctggaa ctggttgccg catggctgcc
     9061 cgaagtggag aaattctcta aggaaatcgg cagacagcag gcgtatatcg acagcttaaa
     9121 ggagcaaatc gggcaggaag ccgactatgc agggcgtatg cgtgatgaaa agtacgagca
     9181 ggaattaaag gtgcagaaag ccaaccagag gatatttgaa ttgcagagaa ccaacgagca
     9241 gatggggcgg ctgctctcaa agataccgcc agaagtgttg gaagaattac agagaacagg
     9301 cagaaacaaa tcaagagaaa ggtaaaaagt gaatgaagaa acaggatttt aaggtgttaa
     9361 agaccaaaga tttatacccg tttcccgaca atccgtttca tgttgtggag gatgaaacgc
     9421 tgtcagagtt agcggaaagc atcaaggaat ttggcattgt cacgccgata atcacacgcc
     9481 cgaaagagga cgggaacggt tatgaagtga ttgcggggca gcggcgtgtc cgtgcgtccg
     9541 aacttgcagg tataaatacc gtgcctgcat ttgtcctgcc cttagaccgt gaccgagcca
     9601 tcatcaccct tgtagacagt aatttacagc gtgaaaatat cctgccatcg gagcgggcgt
     9661 ttgcctacag gatgaaatcc gaagccatga agcggcaggg tttccgcaca gacttaacct
     9721 cgtcacaagt tgtgacgaag ttgcggacgg acgacaaagt ggcacagggc ttcggcgtgg
     9781 gcaggatgac cgtccagcgt tttatccgcc tgacggaact gataccgccg attttgcaga
     9841 tggtggacga ggggaaaatc gccctcacac ctgcggtgga actgtccttt ttgaagaaag
     9901 acgagcagga aaatctcttt gccacgatgg agagcgaaga agcaacgccc tcactctcac
     9961 aggcacagcg gatgaaaagc atgagccaga gcgggcagct tgacatggat atgatatttt
    10021 ccattatgac ggaggaaaag ggaaaccaga aagaaacctt gaaaatcaac acaagcaaac
    10081 tgaaaaaata ctttccgaag gacacaacgc cgaagcagat ggaggaaacc atcatccgac
    10141 ttctggaacg tgagttgcag agaaaacgga gcagggacag ccgctaacct tcttttttcg
    10201 ggaagtaaat gcaggaattt ggaacagaag aaagacagaa ttttaaggag aatgaggtaa
    10261 ggaatgaaag aaatccagta tgagattgta aaggaaatcg ccgtattgtc tgcgagtgac
    10321 agcggctaca caaaggaaat caatcttatt tcatggaacg ggagagagcc gaaatatgac
    10381 atccgcagct tttccccgaa ccgtgagaag tgcggaaagg gtatcacgtt gaacgctgat
    10441 gaagcagcgg cactccttga agcattacag aaagaagtaa acagcgggga ttgatggtat
    10501 ctgattgaca gggcggggcg tttccagacg ttctcctgcc ctgtctggaa aggaagattt
    10561 aagtatgggc gaggatagga aaacaactgg aagaagaaag cgtattgtgc caaaagggca
    10621 agtacaaatg attatcagtc gagaatatat cggcacacag accgttgcag aagcgtttgt
    10681 cccgattatc tccgaggata ttcggaagaa gattgccgag ggcgacacct tcgacaatga
    10741 ggggctgtcc gcttagaatg tacgcaatgg aacatgaaaa cgttaggaca gatatggagg
    10801 ttttacagta tggcaggaat aagaacggag aacaagattt atgaagtcgg catttactgc
    10861 cgcttatcaa aggacgatgg cacggataac gagagtgcga gcattgcgac acaaaaatcc
    10921 atcctcacgg attatgtgaa aaggcaggga tggcatttag caaaaacgta tgttgacgat
    10981 ggctactctg gaacaaattt ccaaagacca agtttccaga atatgattaa ggacattgaa
    11041 aacgggctga taaactgcgt gattacaaaa gatttatccc gtctggggag gaactatctt
    11101 gattgtgggt tatatctgga agtctttttc ccagagcata acgtgaggta tatagcggtc
    11161 aatgacggcg tggacacctt aaacaaatcc gctatggaca tcacgccttt ccgcaacatc
    11221 ctaaacgaaa tgtattctgc cgatgtgtca gtcaagataa aatcgacgta ccgtgcgagg
    11281 tttcagcagg ggaaattcat gggaacaaca gccccttatg gctatgtcaa agaccctgcc
    11341 gaccacaacc atctgctgat agatgacaaa gttgcccatg tggtaaggga gatattcgac
    11401 cttgcgttag cgggcaacgg catcgccaaa atccgcaagc acatcaacaa acagcatatc
    11461 ctacgccctg ccgcttatgc ggtggagcaa ggggcaacag gctatgagcg gtactttgag
    11521 gataacgagg aaaaccgtta tatttggagc gagaacagcg tgaggggcat tttaagaagc
    11581 ccgatatatg cgggcaacct tgcaggctac aagcggattg cagccaacat gaaaagcaag
    11641 aaacgcccct ctaagctgcc cgaagaatgg gaagtgatac cagacaccca tgaggggata
    11701 gtcacgcagg aggaatttga caccgtacag cagcttatga cgagccgcag gcgggagcag
    11761 aacgcagggg gatttgagaa tatcttttcg ggcgttatca agtgtgcgga ctgcggctat
    11821 gcgatgcggg cggcgagtgc caacaggagg aaacgccccg acatcatcga ctgcgtacaa
    11881 tacacctgca ataattatgg cagatatggc aatgttatgt gtaccgcaca cagcattgaa
    11941 gcgagggact tattcaatgc cgtccttgcc gacatcaacc gctttgcgga tatggcggtc
    12001 aatgatgaaa aggcggtcag agccatagaa aggcggctca cggaaacaga ccagagcagg
    12061 gcgaaatcat tagagaaaga aaagaagaag ctgaacaaac gccttgcgga gcttgacagg
    12121 ctgttttcct ctctctatga ggacaaggta atggagcgta tcacggagcg gaattttgag
    12181 atgatgtcgg ggaaatacca gaaagagcag cttgaaattg aagcacggct gaaagaggta
    12241 acggaaacgc tcaatgacag ctatgagaaa tcgcagggcg tgagggactt tctatcccta
    12301 atccgcaact atcaggggct gaaagagttg gacgcaacca tcataaacgc actcatagac
    12361 aagatacttg tttcggaacg tgagaaactt gcggacggga cagtaaggca ggaaatcaag
    12421 atttactata aattcatcgg ctttgtcggt gaattacata tcacacccac aaagcggtgg
    12481 acggcgttaa agcctaaaaa ttgtacggtg tgcggtgttg aatacgtccc cagctctgga
    12541 atatcaaagt attgtcccgc ttgtgctaag aggatacaga gggagaaatc aaacgagagc
    12601 aaacgcagga gtagggagag gaaccgaagg gcatgtattg aactgtccgc aaaaaatgac
    12661 cgactgatat tgtccagcga aatggacgac tgattcgaca aggaaacatg agccaggagg
    12721 tagatattta tcactatatt actaaaggga gcttcgacaa ttacct
//
LOCUS       DATUCG010000067        12709 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.068, whole genome shotgun sequence.
ACCESSION   DATUCG010000067 DATUCG010000000
VERSION     DATUCG010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12709
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.068"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(67..777)
                     /locus_tag="VCA20_001468"
     CDS             complement(67..777)
                     /locus_tag="VCA20_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEP1835276.1"
                     /translation="MKVPKYQLIQNDLRQQIISGKFENGDKFYTESELTKLFNVSSIT
                     VIRAVNELVKDGYLVRQQGKGTFVSRSRKGRLVEFSDIEIFPMDKDKVTVLSCEKGNK
                     PDILEKLNLDKNEFYYKIVRVRAAEDTPYIFHNSYIPQRYIQNPDAPLEHYQSIYQRF
                     KLDYNIHMSEEPFVETNEIVSPSPKEVATHLKLKATEPAVLQNKTTTNSTSGEVMEYT
                     ETYKHWKYYKFEITANHR"
     gene            999..2771
                     /locus_tag="VCA20_001469"
     CDS             999..2771
                     /locus_tag="VCA20_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEP1835277.1"
                     /translation="MKEFYIGDQFYLDGEPFKILSGAIHYFRVHPDDWYHSLYNLKAL
                     GFNTVETYVPWNMHEPRKGEFCYEGILDIERFLKLAQELGLYAIVRPSPYICAEWEWG
                     GLPAWLMKEELRVRSSDSVYLQHLDEYYASLIPKLAKLQLAQGGNVLMFQVENEYGSY
                     GEEKAYLRAVAGLMRKHGLTAPLFTSDGSWRATLRAGTLIEDDVFVTGNFGSKARENF
                     ANMTAFFNEHQKNWPLMCMEFWDGWFNRWGDEIIRREPEEMVDSVMECIELGSLNLYM
                     FHGGTNFGFMNGCSARGQIDLPQVTSYDYDAILDEAGNPTKKFYILQQRLKKVYPELE
                     YAEPLVKEAKAFSDVSLHDKVSLFTTLENVSDCVKGVYPKNMEELDQSTGYILYRTEL
                     ERDKTEAERFRVVDARDRIQIYADGKFVATQYQTEIGDDVELDFKDDKLTLDILVENM
                     GRVNYGHKLTAPTQSKGLGRGAMADLHFIGHWETYPLHLESVEDLDFSKGWEEGQAAF
                     YRYQFELDELADTYLDMTGFGKGVVFVNNVNIGRFWEKGPILYLYIPKGYLKKGANEI
                     VVFETEGKYREKIHFSQRPVIKDL"
     gene            2825..3310
                     /locus_tag="VCA20_001470"
     CDS             2825..3310
                     /locus_tag="VCA20_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEP1835278.1"
                     /translation="MAIIATRIDGRLIHGQVANLWTTKLNIGRIMVIDDAVAQNDIEK
                     QGLKLACPPGVKLSILPIEKAANNIKEGKYDSQRLLIVARRPENFLRLVEYGVELPEL
                     NVGNMSQTPETRSVTRSINVVDKDIADFDALAAKGVKLFAQMVPGDSPKDFMPLLDKV
                     R"
     gene            3349..4251
                     /locus_tag="VCA20_001471"
     CDS             3349..4251
                     /locus_tag="VCA20_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002922767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEP1835279.1"
                     /translation="MMQWWQILLLTLYSAYQICDELTIVSSAGSPVFAGFITGLIMGN
                     MEVGLLIGASLQLVVLGVGTFGGASRIDATSGAVLATAFSISQGLDPEIAISTIGVPV
                     AGLLVYTDILGRFTTTFFAHRVDAAIERFDYAGIERNYLLGAIPWALSRALPVFLALA
                     FGGGLVETLVTTIELPEYKWIAAGLTLAARMLPGLGFAILLHYLPLKRNLHYLAVGFA
                     LTAMLTVLYGNVSALGGAVAGIVGTLPEDAGVSFVNNFKGLSMIGIAIIGAFLSVLHY
                     INSQKVAVVAPSNSESGEIDDDEI"
     gene            4238..5056
                     /locus_tag="VCA20_001472"
     CDS             4238..5056
                     /locus_tag="VCA20_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEP1835280.1"
                     /translation="MTKSNYKLTKEDFNQINKRSLFTFQLGWNYERMQGSGYLYMILP
                     QLRKMYGDGTPELKEMMKLHTQFFNTSPFFHTIITGIDLALEESDGVASKDAVNGVKT
                     GLMGPFAPIGDSIFGSLVPAIMGTVAATMGKEGSPVGIFLWVAVAIAYDIFRWKQLEV
                     AYKEGTKLITTMRDRLTALVDAASVMGVFMMGALIATMINFEVTLAPAIGEKVIDIQD
                     ILNTIFPRLVPAIFTGFVFWLLGRRGMTSTKAILIIIVLALGLSAIGHFLFGMV"
     gene            5056..5457
                     /locus_tag="VCA20_001473"
     CDS             5056..5457
                     /locus_tag="VCA20_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEP1835281.1"
                     /translation="MVRNLVLVSHGIFCEELKKSTEMIMGPQEDIYTVALLPEEGPED
                     FQKKFEETIAGLEDFVVFADLLGGTPANVVSRKLIEGGQFDLYAGMNMPMVIGFLNGV
                     LLGEAVDYVEFGTSNLVHVNSLLTSDEDDDE"
     gene            5805..6806
                     /locus_tag="VCA20_001474"
     CDS             5805..6806
                     /locus_tag="VCA20_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEP1835282.1"
                     /translation="MIEVKTFGEKAKLYCLENKNGMQVTLTDFGARVVEVLLPVEEGG
                     SLRNVSLAAKSDEDYRKTDLYPGSTIIPVAGRISGAQAEIKGTSYHFTENEPGRTLHG
                     GVDTANEQYWNVELDHERNQVTFGMVLKDGFNGFPGDVRIKAIYCLTDKNELTVDYQA
                     VSDKDTIFNPTNHIYFNLTGDFQRSVAEHRIKIAANHYAPLGENNLPTGVLEDVTGTP
                     FDFRDFAPFAQGFDSQYPQNVLVKGYDHPWLLEEVDIPVEVLSPDGKIGLSVKTNQPA
                     VVIYTYNFPVEALACYHGVFSLECQALPNACNVDGFGSILLEQGEEFLSKTTYRFTW"
     gene            6913..7170
                     /locus_tag="VCA20_001475"
     CDS             6913..7170
                     /locus_tag="VCA20_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011074664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HEP1835283.1"
                     /translation="MMTKEQEFLKEFEAWVNTQVMVNEMAVEESRRVLEEDKDERAAD
                     AYIRYESKLDAYRFIQGKFANYHAGKGFHDLPDELFGQRHY"
     gene            7193..7579
                     /locus_tag="VCA20_001476"
     CDS             7193..7579
                     /locus_tag="VCA20_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEP1835284.1"
                     /translation="MGKKKVNRKKQLKKQLADLKRAGRVGLERAAEVVETVAHKAEAV
                     VEHAVEQVKEVVAEVTSSATSLEDFLALPELEGIAAARLETFYEAGIQSVADFANHTE
                     KELLALKGIGPATIKQLKEKGIELKA"
     gene            7820..8212
                     /locus_tag="VCA20_001477"
     CDS             7820..8212
                     /locus_tag="VCA20_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEP1835285.1"
                     /translation="MLHHVEIYVSDLETSRAFYDFLLTKLGYSLYQEWDKGLSYKKAE
                     QYLVFVQTPQDFLEVSYHRCRTGLNHLAFHVGTPDEIDQWRQELLTRRIKLLYDDRYS
                     HAGGSDHYALYLEDPDGIKIELVGEERK"
     gene            8218..8814
                     /locus_tag="VCA20_001478"
     CDS             8218..8814
                     /locus_tag="VCA20_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEP1835286.1"
                     /translation="MTVKQGFDTAKFYYGFPVFILGYKDEQHLYNITTCSSSYSLGDM
                     VVVGMFQGSNAAEQIAKFGEFTLNLTTVEQGYLMEKAGFLHKREKINLLNAEIIPAET
                     VDAPSLVACPITLECRVERVQSFDGYVNFTARVLKRTVDETLLDDKGNFKSSEFNPLL
                     YMGDGHERIYRYLERGTSEKLGTFIKKKRELDRSARLK"
     gene            8823..9395
                     /locus_tag="VCA20_001479"
     CDS             8823..9395
                     /locus_tag="VCA20_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEP1835287.1"
                     /translation="MNLIIIGAQASGKMTIGQEVEKLTDLILFHNHESIDFVTKFFPM
                     SDEARELINDIRMKFFETSAKTDLGLIFTVVIDFNDPADISFLEQIQAIYHSRNQAVL
                     FIELETELQERLRRNVTENRLQHKPWKRNIEWSEKDILDTMKFANFNPEFAPKQLDHY
                     LKINNTHLTANEVAQLIIQKMEELEQEQTK"
     gene            9664..12315
                     /locus_tag="VCA20_001480"
     CDS             9664..12315
                     /locus_tag="VCA20_001480"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEP1835288.1"
                     /translation="MSKELSPKYNPAEVEAGRYQTWLDQDVFKPSGDAEAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLREQG
                     VTRYDLGREKFLDKVWEWKDEYATTIKEQWGKLGLSVDYSRERFTLDEGLSKAVRKVF
                     VELYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALQVAT
                     TRPETMFGDVAVAVNPEDPRYKDLIGKNVILPIVNKPIPIVTDEHADPEFGTGVVKIT
                     PAHDPNDFLVGQRHNLPQVNVMNDDGTMNELAGEFAGMDRFEARKATVAKLQELGALV
                     EIEKRVHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQVTDDKVDFYPPRFNDA
                     YLQWMENVHDWVISRQLWWGHQIPAWYNEAGEIYVGEEAPAGDGWKQDEDVLDTWFSS
                     ALWPFSTMGWPDTEAEDFQSYYPTSTLVTGYDIIPFWVSRMIFQGIEFTGKSPFKNAL
                     IHGLIRDEEGRKMSKSLGNGIDPMEVIEKYGTDSLRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNEGLTLADATANVTKVASGEVGNVTDKWILHNLNETIGKV
                     TENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEAEKVMTRSVLLYTLDQI
                     LRLLHPIMPFVTEEIFAQYAEGSIVVAAYPVVNPAFENAEAHKGVESLKDLIRSVRNS
                     RAEVNVAPSKPITILIKTADAELETFFKANENYIRRFTNPEQLEISSSIAAPELAMSA
                     VITGAEIFLPLADLLNVEEELARLDKELAKWQKELDMVGKKLSNERFITNAKSEVVEK
                     EKEKQADYQAKYDATVGRIEEMKKLVG"
BASE COUNT         3730 a         2252 c         3011 g         3716 t
ORIGIN      
        1 ataacccacc acaaaatgtg ataggttagt taacttaatg gcattgggac gttagtctgc
       61 gtttttttag cgatgattgg ctgtaatttc aaatttgtaa tacttccagt gtttgtaagt
      121 ttcagtatat tccatcactt caccgctagt actgttagta gttgttttgt tttgtagtac
      181 agctggttca gtcgctttta atttcaaatg ggtggcaacc tcttttggag atgggctgac
      241 aatttcattc gtttctacaa atggttcctc ggacatgtgg atattgtagt ccagtttgaa
      301 gcgttgatag attgattgat agtgctcgag tggagcatcg ggattctgga tatatcgttg
      361 agggatatat gaattgtgga agatgtatgg agtgtcttct gctgcgcgga cgcgtacaat
      421 tttatagtaa aactcattct tgtcaagatt tagtttttca aggatatcgg gtttattgcc
      481 tttttcgcac gagagaacag taaccttatc cttatccatc ggaaaaatct caatatcaga
      541 aaattctaca agacgtcctt tgcgtgaacg ggatacaaag gttcctttac cttgttgccg
      601 aacgaggtag ccgtctttaa ctagttcgtt tactgcacga ataacggtga tagagctaac
      661 gttaaatagt tttgttaatt cagattctgt gtaaaatttg tcgccatttt caaatttgcc
      721 agagataatt tgctggcgta agtcgttttg tataagctgg tacttaggta ctttcatact
      781 tatttccttc ctttccatgg ctcattatac taaattaatt gatgaaaatc aaaaagtata
      841 gctggaatcc ataaaaaaaa tagatgtaat ccctttctga ctgatgaaat agatgtgttt
      901 gtaaaaaaaa gtaaaaaact ttaaaaaagt actggacata accacaaata agtgttagta
      961 tattaataga aaggtaagcg gttgcgtcgg aggaagaaat gaaagaattt tatattggag
     1021 accaatttta tttggacggt gaacccttta agattttatc aggcgcgatt cattattttc
     1081 gtgtccatcc tgatgattgg taccattctt tgtataactt aaaggccctt ggttttaata
     1141 cggttgaaac ctatgtccct tggaatatgc atgaacctcg aaagggtgag ttttgttacg
     1201 agggtatatt ggatattgag cgatttttaa aattggctca agaattaggg ctttatgcaa
     1261 ttgtacgtcc ttccccttat atttgtgcgg aatgggaatg gggtggcctc cctgcttggt
     1321 tgatgaagga agagcttaga gtacgttcga gtgattcggt gtacttgcag cacttagatg
     1381 aatattacgc ttctcttatt cctaaattgg ccaagctcca actcgcacag ggtggaaatg
     1441 tacttatgtt ccaagtggaa aatgagtatg gatcttatgg tgaggagaag gcatatttaa
     1501 gagcggtggc tggcttgatg cgtaaacatg gtctaactgc tcccttgttt acctcggacg
     1561 gttcttggcg agcaacatta cgtgcaggaa cactgataga ggacgatgta tttgtaacag
     1621 gaaactttgg ttctaaagct agagagaatt ttgcaaatat gacggcattc tttaatgaac
     1681 atcagaagaa ttggccactc atgtgtatgg aattttggga tggttggttc aatcgttggg
     1741 gcgatgaaat tattcgtaga gagccggagg aaatggttga ttcggttatg gaatgtatcg
     1801 aattgggctc cttgaatctg tacatgttcc atggtggaac aaattttggt ttcatgaatg
     1861 gttgctctgc tcgtggtcaa attgatttgc ctcaggtaac ttcttatgat tatgatgcta
     1921 tattggatga agctggaaat cctactaaga agttttatat tttgcaacaa cgtttgaaaa
     1981 aagtctatcc tgaattagag tatgcagaac cgcttgtaaa agaggcaaaa gctttttcag
     2041 atgtgtcgct tcatgataag gtgagtttat ttactactct agaaaatgta agcgattgcg
     2101 taaaaggggt ttatcctaag aatatggaag aacttgatca gtcaacaggt tatattttat
     2161 atagaacaga gttggagcgg gataagacag aagcagaacg tttccgagtg gttgatgcgc
     2221 gtgaccgtat ccaaatttat gcagatggaa aattcgttgc aactcagtat cagacagaga
     2281 ttggtgacga tgttgaattg gattttaaag atgacaagct aacattagat attttggtag
     2341 aaaatatggg acgtgtcaat tatgggcaca aactaacggc tcctacccag tctaaggggc
     2401 ttggtcgagg tgcgatggct gatttacatt ttattggtca ttgggaaacc tatcctttgc
     2461 atcttgagtc agttgaggac ctggatttta gtaagggctg ggaggaaggt caagctgctt
     2521 tctatcgtta tcagtttgaa ctggatgaac ttgcggatac ttacttagac atgacaggtt
     2581 ttggtaaggg agttgttttc gttaataatg taaatattgg acgtttttgg gaaaaaggac
     2641 caatcctcta tctctatatt ccaaaaggat acttaaagaa aggagcgaat gaaatagttg
     2701 tcttcgagac agaaggaaaa tatagagaga agattcattt ttcacaaaga cccgttataa
     2761 aagacttgta gaaattttaa tcggtttctg ctcaaaagat ttatgaagaa aaggagaaca
     2821 ctgtatggca attattgcta cgcgtattga tggtcgtttg atccacggtc aggttgctaa
     2881 cttatggacg acgaaattga acatcggtcg tatcatggtt atcgacgatg ctgttgctca
     2941 aaatgacatt gaaaaacaag gactgaaatt agcatgtcct ccaggcgtaa aattatcgat
     3001 tttaccgatt gaaaaggctg ctaataatat taaggagggc aagtatgata gtcaacgtct
     3061 cttgattgtt gctcgtcgtc cagaaaactt ccttcgcttg gtagagtatg gggttgagct
     3121 ccctgagttg aacgtaggga atatgtctca aactccggaa actcgttctg taactcgttc
     3181 tattaacgtt gtagataagg atattgcaga ttttgatgcc ttggcggcta agggtgtaaa
     3241 attatttgca caaatggtac ctggcgattc accaaaagac tttatgccat tattggataa
     3301 ggttagataa cattcggata tttttgaaaa atttaaagga ggatcactat tatgcaatgg
     3361 tggcaaatat tacttctaac gctctactct gcttaccaga tttgtgacga gctgacaatt
     3421 gtatcgtcag caggttcacc agtctttgca gggtttatca ctggtttaat catggggaac
     3481 atggaagttg gcttgctaat tggtgcaagt ttgcagctag ttgtcttggg agttggtact
     3541 ttcggtggtg cgtcacgtat tgatgcaaca tctggtgcgg ttcttgcgac agctttctca
     3601 atttcacaag gtttagatcc agaaattgcc atttccacta ttggcgtacc agttgcagga
     3661 ttgttagttt atacagatat tttaggtcgc ttcaccacta ctttctttgc tcatcgtgta
     3721 gatgcagcaa tcgaacgttt tgactatgct ggtattgaac gcaactacct tcttggtgca
     3781 attccttggg cactttctcg tgcacttcca gtttttcttg cccttgcttt tggtggtggt
     3841 cttgtagaaa ctcttgtcac tacgattgag ttgcctgaat acaaatggat tgcagcaggc
     3901 ttaactcttg cagctcgtat gcttccaggt cttggatttg caatcttact tcactatctt
     3961 ccacttaaac gtaaccttca ctacttggcg gtaggtttcg ctctcacagc tatgttgact
     4021 gttctttacg gcaacgtatc agctttgggt ggtgcagttg ctggtatcgt tggcactctt
     4081 cctgaagatg caggcgtaag ctttgtcaat aacttcaaag ggttgtcaat gatcggtatc
     4141 gcgattatcg gtgcattcct ttcagtattg cattatatca atagccaaaa agtagcagtt
     4201 gttgctccat caaattcaga aagtggggaa attgacgatg acgaaatcta attacaaatt
     4261 aactaaagaa gattttaatc aaattaacaa acgtagcttg ttcactttcc aacttggttg
     4321 gaactatgaa cggatgcaag gttctggtta tctctacatg attttgccac aattgcgtaa
     4381 aatgtatgga gatggtactc ctgaattgaa ggaaatgatg aaattgcata ctcagttctt
     4441 caatacttca ccattcttcc ataccatcat cacaggtatt gacttggcac ttgaagaaag
     4501 tgacggtgtg gcttctaaag atgcggttaa cggtgttaag acaggtttga tgggaccatt
     4561 tgctccaatc ggtgactcaa tctttggttc tcttgttcca gcgattatgg ggactgtggc
     4621 tgctactatg ggtaaagagg gatctccagt tggtatcttt ctttgggtag cagtagccat
     4681 tgcctatgat attttccgtt ggaaacagtt ggaagttgcc tacaaagaag gtactaagtt
     4741 gattacaacc atgcgcgatc gcttgacagc tctcgttgat gcagcatctg taatgggtgt
     4801 cttcatgatg ggagccctga ttgcaactat gattaacttt gaagtgactt tggctccagc
     4861 tattggagaa aaagtgattg atattcaaga cattttgaat accatcttcc cacgcctagt
     4921 acctgcaatc tttacaggat ttgtcttctg gcttcttggt cgtcgtggta tgacttcgac
     4981 taaagctatc ttgattatta ttgtgcttgc actcggcttg tcagcaatcg gtcacttcct
     5041 atttggtatg gtataatggt tagaaattta gtattagtaa gtcatggtat cttctgcgag
     5101 gagttgaaaa aatctactga aatgattatg ggtcctcaag aggacatcta cacagtagct
     5161 ttattaccag aagaaggtcc agaagacttc cagaaaaaat ttgaagaaac aattgcaggg
     5221 ttggaagatt tcgttgtctt cgcagactta ttgggcggta caccagccaa cgtcgtatca
     5281 cgaaaattga ttgaaggtgg gcagtttgac ctctatgcag gtatgaatat gccaatggtt
     5341 atcggtttct tgaacggcgt cctgctggga gaggctgtgg attatgtaga atttgggaca
     5401 agcaacctcg tccatgtcaa tagtctatta acaagtgatg aagatgacga tgagtgaaac
     5461 agttcgggga actgtttcag cccgagccta gaaacgggag agcgaggggg agtgggaaag
     5521 aacttgatat gttgaagagt gttcgtcttc ccacccccgc acagttgatt aggttagctt
     5581 tggagtatgt aaaacgaaca aagctaccaa tcaaccactg cgctgagatg ttgcatgaaa
     5641 tctgagaagt ggtggaaact tatcacccgg acagttcggt acatattagt acaagagaat
     5701 tactcgtgtt ccagtttttt gactagtcat ctatttagat aggctttaag tatcctgagg
     5761 gagttgcaat agtgactccc tcatttggct tgtgaggaac agaaatgatt gaagtaaaaa
     5821 catttggtga aaaagcaaaa ctgtattgct tggaaaacaa gaatgggatg caggtaacct
     5881 tgacggattt tggtgccaga gtggtggaag tgcttttgcc tgtggaagaa ggtggcagtt
     5941 tacgaaatgt cagtttggct gctaagtcgg atgaagatta ccgcaagaca gatttgtatc
     6001 ctggttcaac aattatccca gtagcggggc gtatttcagg agcccaagct gagataaagg
     6061 gaacaagcta ccatttcaca gaaaatgaac ctggtaggac tctacatggt ggggttgaca
     6121 cggcaaatga gcagtactgg aacgtggagc tagaccatga gagaaatcaa gtgacctttg
     6181 gtatggtcct aaaagatggt tttaatggct ttcctggtga cgtgaggatc aaggctattt
     6241 attgtttgac ggataaaaat gaattgacag ttgactacca ggctgtatcg gataaggata
     6301 cgatttttaa tccgaccaac catatctatt tcaacttgac tggtgatttt caacggtcag
     6361 ttgccgagca ccgtattaaa attgctgcca atcactatgc tccgcttggg gagaataacc
     6421 tgcccacggg tgtcttagaa gatgtgacag ggacgccgtt tgattttaga gattttgcgc
     6481 cttttgctca gggatttgat agccaatatc ctcaaaatgt gttggtaaaa ggctatgacc
     6541 acccatggtt attggaagag gtggatattc cggtagaagt tttgagtcca gatgggaaaa
     6601 ttggactcag tgtcaaaacc aaccagcctg cggtcgttat ttatacatat aattttcctg
     6661 ttgaagcttt agcctgttac catggtgtct ttagtttaga gtgtcaggcc ttgccaaatg
     6721 catgcaatgt tgatggattt ggttcgattt tgttagaaca aggagaagaa tttttgtcta
     6781 aaacgaccta tcgttttact tggtaaaaaa atggggactc taactgtgtt acagttaggg
     6841 ttttcttgtg ttttctagaa aatatggtat aataaaccgg tgtgtttcaa tcgaagcagg
     6901 cgaaagagga aaataatgac aaaagaacaa gaatttttaa aagaatttga ggcttgggtc
     6961 aacactcagg tcatggtaaa cgagatggcg gttgaagaaa gtcgtcgtgt cttggaagaa
     7021 gacaaggatg aacgtgcagc ggatgcttat attcgttatg agagcaagct ggatgcctat
     7081 cgattcattc aagggaaatt tgccaactac catgctggca aaggttttca tgatttacca
     7141 gatgagttgt ttggtcaaag gcattattaa tcagacggga gagagatgaa aaatgggaaa
     7201 aaagaaagta aatcgaaaaa aacaattgaa aaaacaatta gcagacttga aacgtgcagg
     7261 tcgtgtaggt cttgaaagag ctgcagaggt agtagagaca gttgctcaca aggctgaggc
     7321 tgtagtagag catgctgtgg aacaagtgaa agaagttgta gcagaggtga cttcttcagc
     7381 gacttcattg gaagactttt tggcgcttcc tgagttggaa ggcattgcgg cagcacgttt
     7441 ggaaaccttc tacgaagcag gtattcaatc tgtggctgat tttgcaaacc atacagaaaa
     7501 agagctcttg gctcttaaag gcatcggtcc tgcaactatc aagcagttga aagaaaaagg
     7561 gattgagttg aaagcttaaa tcccttgctt ttcaaggtgc aatttgttac aataaagcca
     7621 ttcagaggtg ttttgatctc catttgtcac aggaagcggt ggtactgaga aatccgcaca
     7681 aatgtcaaaa ctggttgctg atgatgtgaa tcgaataagc aaagtgcaga taatgtatct
     7741 gaacaagggt ggtaccgcgg aataacttcg cccctgtcta gcaagtctag gcggggattt
     7801 tcatttggaa aggagaaaga tgctacatca tgttgaaatt tacgtttctg atttagaaac
     7861 ctcgcgtgca ttttacgact ttctcctgac caagctgggg tactcgctct atcaggagtg
     7921 ggataaaggg ctgtcttata aaaaagcaga gcagtacctg gtctttgtcc aaacgccaca
     7981 ggacttccta gaggtaagtt accaccggtg ccgcacaggt ctcaatcatc tagcttttca
     8041 tgtgggcaca cctgatgaga ttgaccaatg gcgtcaggaa ttgttgacca gacgaatcaa
     8101 attgctgtat gatgatcgct attcacatgc aggaggatca gatcactatg ccctctattt
     8161 ggaagacccg gacgggatta agatagagtt ggtgggggag gaaaggaaat aaaagatatg
     8221 acagtaaaac aaggatttga tactgctaag ttttattatg gtttccccgt attcatttta
     8281 ggctataagg acgagcagca tttatataac attactacat gtagttcctc ttatagtcta
     8341 ggagatatgg tggtggttgg catgtttcaa ggtagcaacg ctgctgaaca aatagccaag
     8401 tttggtgaat ttaccttaaa tcttaccact gtggaacagg gctatttgat ggaaaaagcg
     8461 ggatttttgc ataaacggga aaagataaat ttgcttaatg cagaaattat ccccgctgaa
     8521 acagttgatg ctcccagtct agttgcttgt ccgattacat tagagtgtcg agttgagcga
     8581 gtccaatcat ttgatggtta tgttaacttc acagctcgtg ttttgaaacg tacagtggat
     8641 gaaactttgt tagatgacaa gggaaatttt aaaagttcag aattcaatcc tttgctttat
     8701 atgggagatg ggcatgaaag aatttatcgc tatctagaaa gggggacatc tgaaaaattg
     8761 ggtaccttta ttaagaaaaa aagggagctg gataggagtg cacgcttaaa gtaggaggtc
     8821 atatgaatct aattattatt ggagcacaag cttctggcaa gatgaccatt gggcaggaag
     8881 ttgagaagtt aactgatttg attttgtttc ataatcatga atcaatcgat ttcgtaacaa
     8941 aattttttcc aatgtctgac gaagcgagag aacttattaa tgatattcgt atgaagtttt
     9001 ttgaaacctc tgcgaaaact gatttgggtt tgatttttac agttgttatt gactttaatg
     9061 atccagcaga tatatccttt ttagagcaga ttcaagcgat ttatcatagt aggaatcaag
     9121 ctgttctgtt tattgaattg gaaacagagc tgcaagaacg tttaagaaga aatgtgactg
     9181 aaaatcgtct gcaacataaa ccttggaaaa gaaatattga gtggtctgaa aaagatattt
     9241 tagatactat gaagtttgca aactttaatc cagagtttgc gccaaagcaa cttgaccatt
     9301 atctcaaaat taataatacc caccttactg ctaatgaagt tgctcaactg attatccaaa
     9361 aaatggagga gcttgagcaa gagcaaacga aatagccagc ccaaaggact gactatttca
     9421 aagagaattt atttgctatt cttagctata tagttagtat atatcttcaa acttaaatga
     9481 atctgaaagc ataatccata taagtggaga aatatacgat tcgtgatgag agaatctaaa
     9541 aaacgaaaac agccgaccaa atcgactgtt ttctaggaga ttatatgaaa aagaattggg
     9601 ctatttctag cttgaagata gtgtaaataa attttcttaa agaatactaa aggagaaaaa
     9661 ttaatgtcaa aagaactttc accaaaatac aacccagccg aggtagaggc tggtcgttac
     9721 caaacttggt tggaccaaga tgtctttaag ccgtcaggcg atgcggaggc gaagccttat
     9781 tctatcgtca ttccgccgcc aaatgtaaca ggtaaattgc acttgggaca cgcttgggat
     9841 accacccttc aagatattat tatccgccag aaacgcatgc aaggctttga taccctatgg
     9901 cttccaggta tggaccatgc ggggattgct actcaggcta aggtggagga gcgcttgcgg
     9961 gagcaaggtg tgacacgcta tgatcttggt cgtgaaaaat tcctcgacaa agtttgggaa
    10021 tggaaagatg aatatgctac aaccatcaag gagcaatggg gtaaactagg tttatctgtg
    10081 gattattcac gtgaacgttt cacacttgac gaaggcttgt caaaagctgt tcgcaaggtc
    10141 tttgttgaac tctacaaaaa aggctggatc taccgtggcg aatttatcat caactgggac
    10201 ccagcggctc gcacagccct ttctgacatc gaggttattc ataaggatgt tgaaggtgcc
    10261 ttctaccaca tgaattacat gttggaagat ggttctcgtg cccttcaagt tgcgacaacc
    10321 cgtcctgaga ccatgttcgg tgacgttgcg gttgcggtta acccagaaga cccacgttac
    10381 aaggacttga tcggaaaaaa tgttatcctg ccaattgtga acaagccaat cccgattgtt
    10441 acggatgagc acgcggatcc tgaatttggt acaggggtcg tgaaaatcac acctgcccac
    10501 gatccaaatg acttccttgt cggtcaacgc cacaatctgc cacaagtcaa cgttatgaac
    10561 gacgacggta ccatgaacga gttggcaggc gaatttgcag gtatggaccg ctttgaagcc
    10621 cgtaaggcaa cggttgctaa gttacaagag ctgggtgccc tcgttgagat tgaaaaacgg
    10681 gtgcactccg ttggtcactc agagcgtaca ggtgttgtgg ttgagcctcg cttgtcaacg
    10741 cagtggtttg tgaagatgga ccaattggcc aagaatgcca ttgccaacca agtaactgat
    10801 gataaggttg acttctaccc accacgtttc aatgatgcct atcttcaatg gatggaaaat
    10861 gtgcatgatt gggtcatctc tcgtcagctc tggtggggtc accaaatccc tgcttggtac
    10921 aatgaagcag gcgagatata tgttggcgaa gaagcgccag caggtgatgg ttggaaacaa
    10981 gatgaagatg tccttgacac atggttcagc tctgcccttt ggccattttc aacaatgggt
    11041 tggcctgata cagaggcaga agatttccag agttattacc caacttcaac cttggtaacg
    11101 ggctatgata ttattccatt ctgggtgtct cgtatgattt tccaaggaat tgaatttaca
    11161 ggcaagagcc catttaagaa tgctctgatt cacggactta tccgtgacga agaagggcgc
    11221 aaaatgtcca aatcgcttgg taatgggatt gacccaatgg aagtcattga gaaatacggt
    11281 acagatagtc ttcgttggtt cctctccaac ggctctgccc caggacagga tgtccgtttc
    11341 tcatacgaaa aaatggacgc ttcatggaac ttcattaaca aaatctggaa catttcccgc
    11401 tatatcctca tgaacaatga gggcttgacg ctagctgatg cgaccgcaaa tgtgacaaaa
    11461 gtagcgagcg gtgaggttgg aaacgtgacg gataaatgga tcctccacaa cctcaatgag
    11521 actatcggca aggtgacaga gaactttgac aagtttgagt ttggtgtggc tggtcacatc
    11581 ctctacaact tcatctggga agagttcgcc aactggtatg tggagctgac caaggaagtt
    11641 ctttacagcg acaacgaagc cgagaaagtc atgacccgct ctgtccttct ctacacgctg
    11701 gaccaaatcc ttcgcctcct ccacccaatc atgccgtttg tgacggaaga gatcttcgcc
    11761 cagtacgcag agggctctat cgtggtggca gcttaccctg ttgtcaaccc agcctttgaa
    11821 aacgctgaag ctcacaaggg agtggaaagc ctcaaggact tgatccgttc ggtgcgaaat
    11881 agcagagcag aagtgaatgt tgctccatct aagccgatta ctatcttgat taagacagcg
    11941 gatgctgagc ttgaaacctt cttcaaggca aatgagaact atatccgccg ctttacaaat
    12001 ccagaacagt tggaaatcag ctcaagcatt gctgcaccag aattagccat gtcagccgtt
    12061 atcaccggtg ctgaaatctt cttgccactc gcagacctcc tcaacgtcga agaagagttg
    12121 gctcgactag acaaagaact agccaaatgg caaaaagaac tagacatggt cggcaaaaaa
    12181 ctcagcaacg aacgcttcat caccaacgcc aaatcagaag tcgttgaaaa agaaaaagaa
    12241 aaacaagccg actaccaagc aaaatacgat gcgacagtag gacggattga agagatgaag
    12301 aaattggtgg ggtaaccaat aaagtacgat gacatttgaa tttttgttag ctagagaatt
    12361 gcaggataat ttgacacgac aaacgtatga agaaaatttc gttttcagat aacgattctt
    12421 tattaaaagt tatattttca tcatgaaatt ttgttataat gtaggtgaaa acgaggataa
    12481 atcacagcga ttatgtaaat atcatgaaaa tatttttgaa aaaatgcatg tagctagaaa
    12541 atagttacat gcattttctt catgcgtacg gcgtgataat ttgattaaaa cacggatatt
    12601 ttctgagttt tttgatatat agtcgaatga attaactttc agacaaggaa ctgaggtgta
    12661 ggtagctaga acagcctaaa ggctgttcta ggttagaaat aagtgagtg
//
LOCUS       DATUCG010000068        12643 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.069, whole genome shotgun sequence.
ACCESSION   DATUCG010000068 DATUCG010000000
VERSION     DATUCG010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12643
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.069"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(8..652)
                     /gene="plsY"
                     /locus_tag="VCA20_001481"
     CDS             complement(8..652)
                     /gene="plsY"
                     /locus_tag="VCA20_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEP1835289.1"
                     /translation="MLVNLILLFIAYLLGSIPSGLWIGQTFFNINIREHGSGNTGTTN
                     TFRILGPKAGTIVFVIDFLKGTLAALLPLIFHAQGISPIVFGLMAVLGHTFPIFAQFK
                     GGKAVATSAGMLLGVAPAFCLYLVLIFASSLYLTSMVSFSSVLAAALAILGALLFPVI
                     GIILPSYDWLFTVIIVFLGSFVIIRHKENIQRILKKEENLVPFGLNLTKQKKRV"
     gene            795..2744
                     /gene="parE"
                     /locus_tag="VCA20_001482"
     CDS             795..2744
                     /gene="parE"
                     /locus_tag="VCA20_001482"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEP1835290.1"
                     /translation="MAKKEIDINNYNDDAIQVLEGLDAVRKRPGMYIGSTDGNGLHHM
                     VWEIVDNAVDEALSGFGDRIDVTINKDGSLSVSDRGRGMPVGMHATGKPTVEVIFTVL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEITRDGAVYKQRFEQGGKPVTTLE
                     KIGTAPKSKTGTKVTFMPDDTIFSTTDFKFNTIAERLKESAFLLKQVTMTLTDERTGE
                     QEEYHYENGVQDFVSYLNEDKETLTPVLYFEGEDAGFQVQVAMQYNDGYSDNILSFVN
                     NVRTKDGGTHETGLKLAITKAMNDYARKTNLLKEKDKNLEGSDYREGLSAVLSILVPE
                     EHLQFEGQTKDKLGSPLARPVVDGIVSDKLTFFLLENGELASNLVRKAIKARDAREAA
                     RKARDESRNGKKKKKDKGLLSGKLTPAQSKNPAKNELYLVEGDSAGGSAKQGRDRKFQ
                     AILPLRGKVINTAKAKMADILKNEEINTMIYTIGAGVGSDFTIEDVNYDKIIIMTDAD
                     TDGAHIQTLLLTFFYRYMRPLVEAGRVYIALPPLYKMSKGKGKTEKIAYAWSDGELED
                     LRKDFGKGFILQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVSVLMGD
                     KVEPRRKWIEDNVKFTLEEATAFTK"
     gene            2768..3370
                     /locus_tag="VCA20_001483"
     CDS             2768..3370
                     /locus_tag="VCA20_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEP1835291.1"
                     /translation="MVLENKLGIENSAELARLEEQISKKKAAQLFETGELFQIEVGTF
                     AGLAHIHQALFEDIYDFAGKIRDVNIAKDNFQFAPRIFLEQSLAYIDKLPHETFDAII
                     DKYADMNIAHPFREGNGRSMRIWLDCMLRASLGKVVDWNSIDKDEYFNAMVRSHVSTG
                     ELKYLLLQALTEDLGQATYFKGIDHSYYYEGYNLYRTEDL"
     gene            3403..3906
                     /locus_tag="VCA20_001484"
     CDS             3403..3906
                     /locus_tag="VCA20_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HEP1835292.1"
                     /translation="MPLRGKLTDRFQKQLSEWVRKRKPFKNKQELEEKFDQALKIWQS
                     SKTRLHAENLLSEQGKLEHFLHSTERKLSRMPLGGDKFAAIPGLISMVRSYISRDYSK
                     LPKGTILAIIGALIYFFSPVDALPDFLLGAGLLDDAFVLGACLKLIKSDLSAFRDWQA
                     SQHKLEE"
     gene            3935..4495
                     /locus_tag="VCA20_001485"
     CDS             3935..4495
                     /locus_tag="VCA20_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HEP1835293.1"
                     /translation="MLEKVERLISEINRIHLEYSKDYFETGKVEKVNLKHTFAKVPTQ
                     PILAYRLNLHEAINDYLMRADVKDIAYFYRVKTSESILDKIERFKERSEGYPVNSILN
                     DIFGARMILTSEEIAGVMERLDDWQEKYGLKNWYLRDKDGYIGIHIYFKNKSNFYYPW
                     ELQLWDEKDVEGNIASHIKYKRKFVG"
     gene            4499..6523
                     /locus_tag="VCA20_001486"
     CDS             4499..6523
                     /locus_tag="VCA20_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HEP1835294.1"
                     /translation="MEWKLESDVNDWVKGQLDRIGLRKLQDYNEESGMSSYMKEALRG
                     SAKTESKANFGKPDFHVEKYSRPVIFENKFSAKKLLAENKEGIRQDDKSIGTYAVNGA
                     LYYARNMIASGKYSEVFAIGVAGDNEENVSLKVYYVFGSALNAYKELTTVQTLDFLEN
                     ERSFETFYKNAVLTEEEKHQILIDSQAVLQSYAKKLNRLMHNHNITAPQRVLYVSGML
                     LAMQDIEMLTPNGDKIKLDDGLIPDNLLGKQVKDKHDGELIVTHISNFLENRGIERTK
                     RDLMISSFKEISKDAQRDEKTDLDKEVAKVIDGQASVTKQIFTFIYEYIFKAIDGLGG
                     HIDIMGEMYSEFLKYALGDGKEIGIVLTPPYVTKMMSQILAIKKDNKVMDLATGSAGF
                     LISAMELMIDDANAIFGKVTTAANEAIDALKKNNLLGVELNAEMFTLAATNMILRGDG
                     SSRIEKGSAFNRPESLFENFKADRLLLNPPFSYDENGLPFLEHGLDKMEKGGLGAIII
                     QDSAGSGKATKTAQSILKKHTMLASIKMPVDLFVPMAGVQTSIYIFEAKTPHDVERPV
                     KFIDFRNDGYKRAKRGISEVDSPVQRYQDIIKIYKAGSRAKVEADWDLDAVYIEDFIS
                     LNGNDWNFEQHQVIDTKPTLEDFKKTVADYLSWEVTQLLQQKGEDITKKP"
     gene            6466..7149
                     /locus_tag="VCA20_001487"
     CDS             6466..7149
                     /locus_tag="VCA20_001487"
                     /inference="COORDINATES: protein motif:HMM:NF013579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEP1835295.1"
                     /translation="MGSDPTPPTKRGRYHKKALSPRLAKLDKEFEEAGGEWREYPVSK
                     LFKIKSTLSFNKDMLTVGDGYDYVTRTSQNQGILQSTGFVNQENVNDAGVWSLGLLQM
                     DFFYRNKPWYAGQFVRKVIPLISMTRHYVLYFQTLLNKLKPKLLSGLVRDVDETFLSS
                     HFILPMKNGEIDFLYIENYIKTLEAEHIETLEAYLTVTGLKEYHLTKNDEKILDTFTK
                     LTDTESRVE"
     gene            7155..7679
                     /locus_tag="VCA20_001488"
     CDS             7155..7679
                     /locus_tag="VCA20_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009755322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEP1835296.1"
                     /translation="MGTYTLSDLFYLNSSKRKYNANSIEFGGEFPYVARGESNNGIRG
                     YITEPEEFLNPAGSISFGQDTATMFYQKEAYFTGDKIKILQAKDIELDRFTGQYIITA
                     MRKSFSTFSWGSSSFNEHVLKSVEVSLPTQNNQPDYTFMSDFIKVIEKLVIKDLVEWT
                     DKKIQATKEVVAQH"
     gene            8036..10489
                     /gene="parC"
                     /locus_tag="VCA20_001489"
     CDS             8036..10489
                     /gene="parC"
                     /locus_tag="VCA20_001489"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEP1835297.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQERALPDIRDGLKPVQRRI
                     LYSMNKDGNTFDKGYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQDWKNREILVVMH
                     GNNGSMDGDPPAAMRYTEARLSEMAGYLLADIEKKTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGATGISAGYATDIPPHNLAEVIDAVVYMIDHPTAKLEKLMEFLPGPDFPTGAI
                     IQGTDEIKKAYETGKGRVVVRSRCDIEQLKAGKKQIVITEIPYEVNKAVLVKKIDDVR
                     VNNKVPGIAEVRDESDRTGLRIAIELKKDSDEQTILNYLYKYTDLQINYNFNMVAIDN
                     FTPRQVGLQKILSSYITHRREIIIARSKFDKEKAEKRLHIVEGLIRVISILDEVIALI
                     RASENKADAKENLKISYEFSEEQAEAIVTLQLYRLTNTDIVTLENEEAALREQIQTLA
                     AIIGDERTMFNLMKKELREVKKQFGNPRLSELQDQAETIEIDTASLIVEEETFVSVTK
                     AGYIKRTSPRSFNASTLEEMGKRDDDQLIFLQNAKTTQHLLLFTNLGNVIYRPVHELT
                     DIRWKDIGEHLSQTLMNFDTNEEIIFAELVENFEEGTYFAVTKYGQIKRVERKEFTPW
                     RTYKSKSTKYAKLKDAEDVVITVSPVVLDDIMLITEKGYALRFNIEEVPIIGAKAAGV
                     KAVNLKDGDVVAAAFISNTSSVYLLTQRGSLKRMATEEIPVTSRAKRGLQVLRELKAK
                     PHRVFAAGPVLTDQGDFDLFSTANEDDTTSQILHVQSKTGKLYEVDVTQLSLSERTSN
                     GSFISDTISDEEVFRAWID"
     gene            10870..11487
                     /locus_tag="VCA20_001490"
     CDS             10870..11487
                     /locus_tag="VCA20_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HEP1835298.1"
                     /translation="MKIIKLFLSIKVYLWLVLSFLLLLCGVWGYGYISGEKSNQQEVS
                     VHAAIESVEQVNELVFLNTAVQKIVTAKNYTVLFGQEMPFSEKSALLIIKYKAKFGIK
                     SPVSIEHLSENQYKITLPAFEVIGLELDAEEPYTLYDKSGEILSFATEEIDTAQLITD
                     EFQLVEQEKFLADYQDDIKESAKTYYQNLFSAISPEIQLEFVFKE"
     gene            11598..12620
                     /locus_tag="VCA20_001491"
     CDS             11598..12620
                     /locus_tag="VCA20_001491"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEP1835299.1"
                     /translation="MTVNIEWENLGFSYMKLPYRFIAHFKNGQWSEGQLTEDAELHIS
                     ESSPALHYGQQAFEGLKAYRTKEGKLQLFRPDQNAERLQRTADRLLMEPIPTDLFIKA
                     CKEVVKANADYVPPYGTGGTLYLRPLLIGVGDIIGVKPAEEYIFTVFAMPVGYYFKGG
                     LAPTNFLIQDKYDRAAPNGTGAAKVGGNYAASLLPGQYAKKQGFSDVIYLDPATHTKI
                     EEVGSANFFGITADNEFVTPISPSILPSITKYSLLYLAEHRLGMKAVEREVLVKELHQ
                     FVEAGACGTAAVISPIGGIQIADKLHVFHSETEVGPVTRKLYEELTGIQFGDIEAPEG
                     WIVEVD"
BASE COUNT         3842 a         2529 c         2901 g         3371 t
ORIGIN      
        1 cgagtggtta aactcgcttt ttctgtttgg ttagattgag tccgaatggg acaagatttt
       61 cttctttttt caaaatccgc tggatatttt ccttgtgacg gatgatgaca aaactgccaa
      121 gaaagacgat aatcactgta aagagccagt cgtagcttgg caggatgatt cctattactg
      181 gaaatagcag agctcctaag attgctaaag cagcagccaa tacgctagag aaggaaacca
      241 tggacgtcaa gtagagtgaa ctggcaaaga ttagaacgag atagaggcaa aaagcaggtg
      301 cgacgcccag aagcatacca gcagatgttg caaccgcctt tccacctttg aattgggcaa
      361 agatagggaa agtgtgcccc agtacagcca ttaacccaaa tacaatcgga gaaattcctt
      421 gggcatgaaa gatcagcgga agcaaggctg ccaaagttcc cttgagaaaa tcaattacaa
      481 atacaatcgt accagccttt ggacctaaaa tacggaatgt gttggttgtt ccagtatttc
      541 ccgaaccatg ttcacgaatg ttgatattga aaaaagtttg acctatccaa agtcctgacg
      601 ggatagatcc caataaatat gcaataaata gtaaaataag attgactaac atgttttcat
      661 tatagcatat tttcatctaa tttagtccaa aatcttattt tgtgagctgt ctcagccgat
      721 aggtaacaga aatattttgc aaaaaagcaa aaaaacttgt aatatagaaa ggatgataag
      781 tatggaggtc aattttggct aagaaagaga ttgatataaa taattacaat gacgatgcca
      841 ttcaggtatt ggaaggccta gatgccgttc gcaaacgccc tggtatgtac atcggatcca
      901 cagacggaaa tggcttgcac catatggtct gggagattgt cgataacgct gtcgatgagg
      961 ccctgtctgg tttcggtgac cggattgatg tgaccattaa caaggatggt agcctgtctg
     1021 tttccgaccg tgggcgtggg atgcctgttg gtatgcacgc aacaggcaag ccaactgttg
     1081 aagtcatctt caccgttctc cacgcaggtg gtaagtttgg tcaaggtggc tacaagactt
     1141 caggtggtct ccacggggtt ggttcttcgg tggtcaatgc cctgtctagc tggttggaag
     1201 ttgagattac ccgtgacggt gctgtttaca agcaacgatt tgagcagggc ggaaagccag
     1261 tcacgaccct agagaagatt ggtaccgctc caaaatcaaa aacaggtaca aaagttacct
     1321 ttatgcctga cgataccatc ttttcaacga cggatttcaa gttcaacacc attgccgaac
     1381 gcctgaaaga atcagccttc ttgctcaaac aagtcaccat gaccttgaca gatgagcgga
     1441 ctggtgagca ggaggagtat cactatgaaa atggcgttca ggactttgta tcctacctca
     1501 acgaagacaa ggaaactctg acaccagtcc tctattttga aggagaagat gcagggttcc
     1561 aagtccaagt ggccatgcag tacaacgatg gctattcaga caacatcctc tccttcgtta
     1621 acaacgttcg gaccaaggac ggcggtaccc acgaaacagg tcttaaacta gccattacta
     1681 aggccatgaa cgactatgct cgtaagacca acttgctcaa ggaaaaggac aagaacttgg
     1741 aaggctctga ctaccgtgaa ggtttgtctg cggtcctttc catccttgtc ccagaagagc
     1801 atttgcagtt tgaagggcag accaaggaca agctgggcag cccgcttgct cgtcctgtgg
     1861 tggacggcat tgtatcggac aaattaacat tcttcctttt ggaaaatggg gaattagcct
     1921 caaatcttgt ccgtaaagcc attaaggcgc gtgacgcccg cgaggctgcg cgtaaggccc
     1981 gtgacgaatc ccgaaatggt aagaaaaaga agaaggacaa gggccttctg tcagggaaac
     2041 tcactccagc ccagtccaaa aatccagcca aaaacgaact ctatctggtc gagggagatt
     2101 cggccggagg ctctgccaaa caaggccgtg accgcaagtt ccaagccatc ctacctttgc
     2161 gtggtaaggt tatcaacact gccaaggcta aaatggcgga cattctcaaa aatgaggaaa
     2221 tcaacaccat gatttacacc attggtgcag gtgttgggtc ggatttcact atcgaagatg
     2281 tcaactatga caagattatt atcatgaccg atgcggacac ggacggtgct cacattcaga
     2341 ccctcttgct gaccttcttc tatcgctata tgcgaccgct agtagaagca ggccgggttt
     2401 acatcgccct tccgcccctc tacaagatgt ccaaaggcaa gggtaagaca gaaaagattg
     2461 cctacgcctg gtctgacgga gaattagaag atctccgcaa ggattttggc aagggcttta
     2521 tccttcaacg ctacaagggt cttggtgaga tgaatgccga tcagctctgg gaaacaacca
     2581 tgaaccctga aacccgtact cttatccgtg tcaccattga agacctagcc cgtgctgaac
     2641 gccgtgtatc cgtcctcatg ggcgacaagg tcgagccacg ccgcaagtgg attgaagaca
     2701 atgttaagtt taccttggaa gaggcgacag cttttaccaa ataatctcag ggaagttgag
     2761 gtgccatatg gttttagaaa ataaattggg gattgaaaat tcggctgaat tagcccgtct
     2821 tgaagaacaa atcagcaaga aaaaagcagc tcaactgttc gaaactggtg agttattcca
     2881 gatagaagtt ggaacctttg caggtttggc acacattcat caggctttat ttgaggatat
     2941 ttacgacttt gcagggaaaa ttcgtgatgt caacattgcc aaggataatt ttcaatttgc
     3001 tccacgaatc tttcttgagc agtctttggc ttatattgac aagctaccac atgaaacctt
     3061 tgatgcgatt attgacaagt acgcagatat gaacatcgca catccattcc gagaaggaaa
     3121 tggacgcagt atgcgtatct ggttggattg tatgctacgg gctagtttgg gcaaggtggt
     3181 tgattggaat agcattgata aagatgaata tttcaatgca atggttagaa gccatgtgtc
     3241 aacaggagag cttaaatatc tgttattgca agctttaacg gaagatcttg gtcaggctac
     3301 ttatttcaaa ggcatcgacc attcttatta ctatgagggt tacaacctat atcgtactga
     3361 ggatttgtaa atctagcata aacaaatgat gtgaggtgtt ttatgccact aagaggaaaa
     3421 ttaactgacc gttttcaaaa acagttgtct gaatgggttc gaaagcgcaa gccatttaaa
     3481 aacaaacagg agttggaaga gaaatttgac caagcattga agatttggca atcttccaaa
     3541 actcggctcc atgcagaaaa cttgctatct gagcaaggaa aattagaaca ttttttacat
     3601 agcacggaac ggaaactgtc tcgtatgcct cttggtggag ataagtttgc ggccattcca
     3661 ggcttgattt ccatggtgcg aagctatatc agtagagatt acagcaagtt acccaaaggg
     3721 acaatcctcg caattatagg tgctttgatt tatttcttta gccctgttga tgccttgcct
     3781 gacttccttc tgggagctgg tttgttagac gatgcttttg ttcttggtgc ttgtctaaaa
     3841 ctcatcaagt cagatttaag cgccttccgt gactggcaag caagccagca caagctagaa
     3901 gagtagctct gctattttca aagggagaaa gaagatgtta gagaaagttg aacgcttaat
     3961 aagtgaaatt aaccgtattc atctggagta ttcaaaggat tattttgaaa caggtaaggt
     4021 tgaaaaagtc aatcttaagc atacctttgc caaggtacct acccagccta ttctggctta
     4081 tcgcctcaat ttacacgaag ctatcaatga ctatttgatg cgtgctgatg tcaaggatat
     4141 tgcctatttt taccgtgtca aaacatcgga atcgattttg gataagattg agcgttttaa
     4201 ggagagaagc gaaggttatc ctgttaattc cattctcaat gatattttcg gtgctcgtat
     4261 gattttaact tctgaggaaa ttgcgggggt tatggagcgt ctggatgact ggcaggaaaa
     4321 atatggtctg aaaaactggt acctgcgaga taaggacggc tatattggca ttcatattta
     4381 tttcaaaaat aagagcaatt tctactaccc atgggaattg caactgtggg acgagaaaga
     4441 tgtcgaaggc aatattgcca gccatatcaa gtataagagg aagtttgtgg ggtaaaaaat
     4501 ggagtggaaa ttagagtcag atgttaatga ctgggtaaaa ggtcagttag accgtattgg
     4561 attacgaaag ttgcaagact acaatgaaga atcaggtatg agctcctata tgaaagaggc
     4621 tctgaggggg tctgcgaaaa cggagtctaa agctaatttt ggtaaaccag actttcatgt
     4681 agaaaaatat agtcgtcctg ttatctttga aaataaattt tcagcaaaaa aattacttgc
     4741 tgaaaataaa gagggtatta gacaagatga caagtcaatt ggcacttatg cagttaatgg
     4801 tgctctgtat tacgctcgaa atatgattgc ttctggtaaa tacagcgagg tctttgcgat
     4861 tggtgtggct ggtgacaatg aagaaaatgt ttctctcaaa gtttactatg tcttcggttc
     4921 tgctctaaat gcctataaag aattaacaac cgttcaaact cttgattttc ttgaaaatga
     4981 gaggagtttt gaaacgtttt acaagaacgc tgttctgaca gaagaagaaa aacatcagat
     5041 tttaattgat agtcaagcgg tcttgcaaag ttatgccaag aaactcaatc gcctgatgca
     5101 caatcataat atcactgctc cacagagagt cctctatgta tctggtatgc ttttggccat
     5161 gcaagatatc gaaatgctta ctcctaatgg tgacaaaatt aagttagacg acggtctaat
     5221 tcctgacaat ttattaggga aacaagttaa agacaagcat gatggcgaat tgattgtaac
     5281 gcatatttca aattttctag aaaacagagg gattgagaga accaagcgtg acttgatgat
     5341 ttcttctttc aaggaaattt caaaagatgc tcagcgtgac gaaaagacag accttgataa
     5401 agaagttgcc aaggtgattg atggtcaggc aagtgtgacc aaacaaattt tcacctttat
     5461 ctacgaatac atcttcaaag ccattgacgg attaggtggt catattgata ttatggggga
     5521 aatgtattct gagtttctca agtatgctct cggagatggt aaggaaattg gcattgtctt
     5581 aacgccgcct tatgtgacta agatgatgtc acagatttta gcaatcaaaa aagataacaa
     5641 agtaatggac ttggcgacag gttcagcagg tttcttgatt tctgcaatgg aattgatgat
     5701 agatgatgct aatgctatct ttggtaaggt gaccacggcc gcaaatgaag ctattgatgc
     5761 actcaagaaa aacaatcttc tcggtgttga gctaaatgca gagatgttta ccttggcggc
     5821 gaccaatatg attttacgag gtgatggctc aagtcgtatt gagaaaggtt cagcctttaa
     5881 ccgcccagaa agtctctttg aaaactttaa ggctgaccgt cttttgttga atccaccttt
     5941 ttcttacgat gaaaatggct tgcctttctt agaacatgga ttagataaga tggaaaaagg
     6001 tggcttaggt gcgattatca tccaagattc agcaggttct ggtaaagcta ccaagacagc
     6061 ccaatctatt cttaaaaagc ataccatgct tgcctcaatc aagatgccag tagatctttt
     6121 cgtgccaatg gcaggagtgc agacgtcaat ttatattttt gaagctaaga cccctcacga
     6181 tgtagaacgc cctgtcaaat tcattgactt cagaaatgac ggatacaagc gagctaaacg
     6241 tggaatttca gaagtggata gccctgttca acgttatcaa gacatcataa agatttacaa
     6301 ggcaggcagt cgagctaaag tagaggcaga ttgggatttg gatgctgtct atatcgagga
     6361 ctttatcagt ctgaatggta atgattggaa ttttgaacag caccaagtca ttgataccaa
     6421 accaacctta gaggatttca aaaagaccgt tgctgactac ctttcttggg aagtgaccca
     6481 actcctccaa caaaaagggg aagatatcac aaaaaagccc taagccctcg cctagccaaa
     6541 ctagacaaag agtttgaaga ggcaggggga gaatggcggg agtatcctgt ttctaagttg
     6601 tttaaaatta aaagtaccct tagttttaat aaagatatgc ttacagtcgg tgacggatat
     6661 gattatgtta caagaacttc tcaaaatcaa ggtattttac agtcaacagg atttgtcaat
     6721 caagaaaatg ttaatgatgc aggtgtatgg agcttaggtc tgttacaaat ggattttttc
     6781 tatcgcaata aaccttggta tgcaggccag tttgttcgga aggtaatccc tttgatatcc
     6841 atgacaagac attatgttct ttacttccaa actttactaa ataaattaaa accaaaattg
     6901 ttatcagggc ttgtacgaga tgttgatgaa acctttttat catcgcactt catactacca
     6961 atgaaaaatg gtgaaattga ttttctgtat attgaaaatt acataaagac gctcgaggct
     7021 gaacacatcg agacgctcga ggcttaccta acagtaactg gacttaaaga gtatcatctt
     7081 acaaaaaatg atgagaaaat acttgacaca ttcacaaaac tgaccgatac tgagagtaga
     7141 gtagagtaga gtatttgggt acttataccc tatcggattt attttatctc aacagtagca
     7201 agaggaaata taatgcaaat tcaattgaat ttggaggaga atttccatat gttgcgagag
     7261 gagaaagcaa taacggtatc agagggtata ttactgaacc agaagagttt ttaaatcctg
     7321 caggttcaat cagttttggg caagatacag caacgatgtt ttatcaaaag gaagcttatt
     7381 ttactggaga taaaattaaa attttacaag caaaagacat tgaactagat agatttactg
     7441 gtcagtacat tataactgct atgagaaaaa gtttttctac attcagttgg ggtagtagtt
     7501 cttttaatga acatgtactg aaaagcgttg aagtttcttt acctacccaa aacaaccaac
     7561 ccgactacac tttcatgtcc gacttcatca aagttattga aaaactggta attaaagact
     7621 tggttgaatg gacagacaag aaaattcaag cgacaaaaga ggttgttgct cagcactaac
     7681 atactgctac ggcatacagg cgagcgtgag cgagctcaaa atggataatc gccgccgtag
     7741 tgaaagaagc taataacaaa gttattggct tcagggaagt tggaagactt tggcttccaa
     7801 cttaggaatg gaactccaaa ggaggtcgct tccgtcccca ctacctgagg cgattatcaa
     7861 aaaaagtaaa aaagaaaact ttttcgacaa gaaacaaaga aaggaatgtt cggttgctag
     7921 aaacaactga acacgcccta tagactgtgt caaaaagata aatcgtctag aaaatcagga
     7981 ttttctgccg atttcttatt ttgactttgt cttttacggg ctttgtatct taattatgtc
     8041 taacattcaa aacatgtccc ttgaggacat catgggagag cgttttggtc gctactccaa
     8101 atacattatt caggagcggg cattgccgga cattcgtgac ggtctcaagc ccgtgcaacg
     8161 tcggattctt tattccatga ataaggacgg caatactttt gacaagggct accgtaagtc
     8221 cgccaagtca gtcgggaata tcatggggaa tttccacccg cacggcgatt cttctattta
     8281 tgatgccatg gtccgcatga gtcaggactg gaagaaccgc gagattttgg tggtgatgca
     8341 cgggaacaac ggttccatgg acggcgatcc gccagcggcc atgcgttaca ctgaggctcg
     8401 cctgtctgaa atggctggtt atctgctggc cgatatcgag aaaaagaccg tgccatttgc
     8461 ctggaacttt gacgatacgg aaaaagaacc cactgtgcta ccagctgcct tccctaatct
     8521 cttggttaat ggagcgacag gaatttcagc agggtacgcg actgacattc cgccacataa
     8581 tctggcggag gtcatcgatg ccgttgtcta catgattgat catccgacag ctaaattaga
     8641 aaagctaatg gagttcttgc ctggaccaga cttcccaaca ggtgccatta tccaagggac
     8701 tgacgaaatc aagaaagcct atgaaacagg taagggccgt gtcgttgttc gtagccgttg
     8761 tgatattgaa caactcaagg ctggcaagaa acagattgtc attactgaga ttccttacga
     8821 ggtcaacaag gctgttttgg tcaagaagat tgatgatgtc cgtgtcaaca acaaggtgcc
     8881 tggtattgcc gaggtgcgtg atgagtcaga tcggacaggt ctacggattg ccattgagct
     8941 gaaaaaagat agcgatgagc aaaccattct caactacctc tacaaataca ccgacctaca
     9001 aatcaattac aactttaaca tggtggcgat tgataatttc acaccgcgtc aggttggctt
     9061 gcagaagatt ctgtccagct acatcaccca ccgccgtgag attatcattg cccgttctaa
     9121 gtttgacaag gaaaaagcag agaaacgcct tcacatcgta gagggtttaa tccgcgtcat
     9181 ttccattttg gacgaggtta ttgcccttat tcgtgcttct gaaaacaagg cggatgctaa
     9241 ggaaaacctg aaaatcagtt atgagttcag cgaggagcag gcagaagcaa tcgtgacctt
     9301 gcaactctac cgcttgacca ataccgacat tgtgaccttg gaaaatgaag aagcagccct
     9361 tcgcgagcag attcagacct tggcagctat tatcggcgat gagcggacca tgttcaatct
     9421 tatgaagaag gaactgcgtg aggtcaagaa gcagttcggc aatcctcgtt tgagtgaatt
     9481 gcaggatcag gcagaaacaa ttgagattga tactgccagt ctgattgtcg aggaagaaac
     9541 ttttgtcagc gtgactaagg caggttacat caaacggaca agtccgcgtt ctttcaatgc
     9601 ctcaacgctg gaagaaatgg gcaagcggga cgatgaccag ctgattttct tgcagaatgc
     9661 caagacaacc cagcacctct tgctctttac caatcttgga aatgtcatat accgacctgt
     9721 ccatgaactg acagatattc gctggaagga cattggtgaa cacctgagcc agaccctgat
     9781 gaactttgat accaatgaag agattatctt tgctgaattg gtggagaatt ttgaagaggg
     9841 gacttatttc gcggtgacca aatacggtca aatcaagcgt gtggagcgga aggaatttac
     9901 accatggcgg acttacaagt ccaagtcaac caagtatgcc aaactcaaag atgcggagga
     9961 tgtggtcatt accgtatcac ctgtggtctt ggacgacatt atgctcatca cagaaaaggg
    10021 ctacgctctg cgatttaaca tcgaagaagt gccaatcatc ggtgccaagg cggctggtgt
    10081 caaggcggtc aacctcaagg atggggatgt ggtcgcggcg gcttttatca gcaataccag
    10141 ctccgtctat ctccttactc agcgaggtag tttgaagcgg atggcgacgg aggaaatccc
    10201 tgtgaccagc cgtgccaagc gtggtttaca ggtgcttcgc gaactcaagg ctaagcctca
    10261 ccgtgtcttt gcggcaggtc cagtcttgac ggatcagggt gatttcgatc tctttagcac
    10321 ggcaaatgaa gatgatacga caagtcaaat ccttcacgtg cagtccaaaa caggcaaact
    10381 ctatgaggtt gatgtgaccc agcttagcct gtctgaacgc actagcaacg gtagcttcat
    10441 ttcggatacc atttctgatg aagaagtctt cagagcctgg atagactaaa aagatgggag
    10501 tggaaaaaga actcgactaa gtcaaaaaga gttcgtcaac aatttttcgt tttaagttgt
    10561 tgagctgaaa cagtctatcc ccagactgtt tcactcccac ccccgcacag ctcaaactgt
    10621 ctgggagaca gtttgaggtt ggagataaag cgaactttgt tcgcatcagt cgtataggtt
    10681 agatttggag tgtaaaacac gaattgctga gatttgcttc gcaaatctta tctccaacct
    10741 ttaacagtcc actggactgt taaacccaat caaccactgt gctgagatgt tgacacgaac
    10801 actgagaaga gggtcagggc tttttgccca gcctcatctt tttgctatac tgtaggggag
    10861 gtaaatttta tgaaaattat caaattattt ttaagtataa aagtctatct atggcttgtt
    10921 ctgtcttttc ttctgttatt gtgtggtgta tggggatacg gctacatttc tggtgaaaag
    10981 agtaatcagc aggaagtgag tgtccatgcg gctattgagt ctgtcgagca agtcaatgaa
    11041 ctagtatttc taaatacagc tgtccagaaa attgtgaccg ctaagaacta cacggtctta
    11101 tttggtcaag aaatgccctt ctcagaaaaa tcagccctct taatcattaa gtacaaggca
    11161 aaatttggta tcaaaagtcc tgtatccatt gaacatcttt ctgaaaatca gtacaaaatt
    11221 acccttccag cctttgaagt tattggattg gaattagatg cagaggaacc ttatacttta
    11281 tacgataaga gtggtgagat actgagtttt gcgacagaag aaatcgatac tgcccaactt
    11341 atcacagatg aatttcagtt ggtagagcaa gaaaagtttt tagcagatta tcaggatgac
    11401 ataaaagagt cagccaaaac ctactatcaa aatttattta gtgctatctc acctgaaatt
    11461 caactagaat ttgtttttaa agagtaagag tctggtttac caggcttttt cttatattca
    11521 caaaattttc tgaattttta attgaaaatc actttcaaaa gtagtaaaat aataaaaaat
    11581 gaatttggag aaactatatg acagtgaaca ttgagtggga aaaccttggt ttttcttata
    11641 tgaaactacc ttaccgcttc atcgctcatt tcaaaaacgg ccaatggtct gaagggcagc
    11701 tgaccgaaga tgcggaactg cacatttcag aaagctcgcc agctcttcac tatggccagc
    11761 aggcttttga aggcctcaag gcctatcgca ccaaggaggg aaaactccag ctctttcgtc
    11821 cagaccaaaa cgctgagcgc ctacaacgga cagctgaccg cttgctcatg gagcctatac
    11881 cgacggacct tttcatcaag gcttgtaaag aagtggtcaa agccaatgca gactacgttc
    11941 ctccctatgg aactggtggt accctttatc tccgtccgct cctgataggc gtcggcgaca
    12001 tcatcggcgt aaaaccagca gaagagtaca ttttcacagt ttttgctatg ccggtcggct
    12061 actacttcaa gggtggattg gctccgacca acttcctcat tcaggacaag tacgaccgtg
    12121 cggcaccaaa tggaacaggg gcagccaagg tcggtggtaa ctacgcggcg tcgcttttac
    12181 cgggccaata tgccaaaaag cagggcttct cagatgtcat ctacctggac ccagctaccc
    12241 acaccaagat tgaagaagta gggtctgcta atttctttgg tatcacagct gataacgagt
    12301 ttgtcacacc gatttcacca tcaatcttgc cgtctattac caagtattct ctcttgtact
    12361 tggcagagca tcgtctgggt atgaaggctg tggagcgtga agtccttgtc aaagaattac
    12421 accagtttgt cgaagcagga gcttgcggaa cagctgcagt catctcacca attggtggta
    12481 tccagattgc agacaaactc catgttttcc atagtgaaac agaagtagga cctgtcactc
    12541 gtaaattgta tgaagaatta acaggtatcc agtttggtga tattgaagct cctgaaggct
    12601 ggattgtcga agtagattaa tttaagaagg cttgcacttg caa
//
LOCUS       DATUCG010000069        12517 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.070, whole genome shotgun sequence.
ACCESSION   DATUCG010000069 DATUCG010000000
VERSION     DATUCG010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12517
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.070"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            72..1073
                     /gene="ruvB"
                     /locus_tag="VCA20_001492"
     CDS             72..1073
                     /gene="ruvB"
                     /locus_tag="VCA20_001492"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEP1835300.1"
                     /translation="MTNRILDMEQMQEEEYVERTLRPQKLNEYIGQDKVKDQLKIFIE
                     AAKLRDEALDHTLLFGPPGLGKTTMAFVIANELGVNIKQTSGPVIEKAGDLVALLNDL
                     EPGDVLFIDEIHRMPMAVEEILYSAMEDFYIDIMIGSGEANRSVHLDLPPFTLIGATT
                     RAGMLSNPLRARFGITGHMEYYELADLTEIVERTADIFDMEITHEAAIELARRSRGTP
                     RIANRLLKRVRDFAQIMGDGLIDDSITDKALTMLDVDREGLDYVDQKILRTMIEMYGG
                     GPVGLNTLSVNIAEERETVEDMYEPYLIQQGFLMRTRTGRVATAKAYEHLGYPYTEK"
     gene            1141..1776
                     /locus_tag="VCA20_001493"
     CDS             1141..1776
                     /locus_tag="VCA20_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEP1835301.1"
                     /translation="MTPTFIWDLDGTLLDSYEAILAGIQETYEQFGLSFDREEVRNFI
                     LRYSVKDLLVRDADKYGLDSDELNRVRATSLKEKNTQIPLMAGAREILDWTAEKGIQN
                     FVYTHKSDNTFQVLEDLSVRQHFTEILTSDSGFARKPSPEALLFLIEKYGLNKENTYY
                     IGDRLLDVETAVNAGIHSINLQIDGLEKNWKIVSLLDIKQILTDKSSHNNL"
     gene            2023..2940
                     /locus_tag="VCA20_001494"
     CDS             2023..2940
                     /locus_tag="VCA20_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEP1835302.1"
                     /translation="MNDKEFGQRVRQLRESASMTREQFCDDELELSVRQLTRIEAGTS
                     KPTFSKIQYIATRLGMGLYELMPDYVSLPERYSKLKFDVLRTPTYGNEDLAEKRDAMM
                     TEIYDDYYDELPEEEKIVVDTLCSLFDVLDTDSQEYGKEILDDYLHQSYHRSKLSIND
                     LMILRLFIEHCQLEDLSVGTSNYALFTDLIEKLPQSIYDVHSESLFIVRDLFLAIVRI
                     LFSKELYNHIPVYIEKIENIMELSQDFQKKPILNLVKWKYELKVQHNHEIAERYYNEA
                     ITFASLLNQFHLKEKLQMEWEKDTQSLKR"
     gene            3205..4425
                     /locus_tag="VCA20_001495"
     CDS             3205..4425
                     /locus_tag="VCA20_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEP1835303.1"
                     /translation="MLENWLNTKQGQVFHYKTEKIEYALELLGNPQFAVPVIHVAGTN
                     GKGSTIAFMRQLFQTHGLRVGSFVSPHMVSVHDRICIDSQPISDHDFQHYLQKVYDLE
                     QEVVTRYEPFRYFEVMVLIMFLYFQDQQLDVALVEVGIGGLLDTTNVVAPALSVITSI
                     GMDHQDLLGSTLREIAEQKAGIIKESVPVVLGPLSSETTAICRHIAQDKQASVHQFGQ
                     EFTYKAGQFSNTDIDLSELVLGLAGHHQEENAAVALQTFLLYMTNIQKDIQSQLIQQA
                     LAQTSWPGRLELVAQEPKIYLDGAHNVPAIERLIEFIQVQEEPVTILFSALRRKDFQE
                     MLELLEEKSPHTPLVLTSFAYDGALSEENRQGRDYVENYQQFIEDWQSSEQGILIVTG
                     SLYFISEVRRILKK"
     gene            4616..6139
                     /locus_tag="VCA20_001496"
     CDS             4616..6139
                     /locus_tag="VCA20_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol oxidase"
                     /protein_id="HEP1835304.1"
                     /translation="MTELDVVSDVVTHPEETYRRSRRASRRQQVQLNGEFHERVLKTK
                     YWPALVWSLVLSIFSVANPLLMPFATNIQTQNLYAGMAMANGQIPYGDFFGTSGLLFY
                     LLAFLGHLGGTFIIFGILQFIALLIAGVYFYKIVAYFSQSEHLATSSSHWFYVFIFAL
                     GFGGMYAEMFALSFLLTSVWFLVRYFENAVRDEAFILYGIDAALVFLIYPKSLILWLV
                     AGLVLFVFNIQHRQVTRGIYQLLATIFGFLLILYAVGYYAFEAQILGTAIQQTFLYNL
                     QLDFHHSYLYLALAIVSVFLFLSGFFKSFIQMVFSFKQGRHTYIKVLLLLTFLVQCVF
                     IVGNANFQWSQLILLLPYGFAMSVVYLRDEDVEDYRGYLRRQFFLPLAICLGIIAQPA
                     YLYLVQGDLRTDREQVANYIGEQTKDSDKIYVWDNSASIYLSSQRLSAATITTAEPYL
                     NTDDNKNSLMYDINKNEAKFVVVNKTLPILDEIKTNLESQYQSVQTTDYFTIYQQNE"
     gene            6257..8194
                     /gene="mutL"
                     /locus_tag="VCA20_001497"
     CDS             6257..8194
                     /gene="mutL"
                     /locus_tag="VCA20_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEP1835305.1"
                     /translation="MSHIIELPEILANQIAAGEVIERPASVVKELVENSIDAGASQIE
                     ISVEEAGLKTIQITDNGEGIAPDEVALALRRHATSKIKSQSDLFRIRTLGFRGEALPS
                     IASVSHMIIETATADSAHGLHLEAKGGVIEKEEPVSRPVGTQITVSDLFYNTPARLKY
                     VRSQQAELSHIVDVVNRLSLAHPEIAFALVNEGRELIRTAGTGKLRQAISGIYGIASA
                     KKMVEIEAEDLDFQISGYVSLPELTRANRNYISIFINGRYIKNFLLNRAILEGYGSKL
                     MVGRFPLAVISIEIDPYLADVNVHPTKQEVRISKEKELMTLIREAISQALKEQDLIPD
                     ALENLAQSSTRPKVKAEQGTLPLREPKIYYDTIKQDFFLKPDVVAEDVKPLEEDRQEI
                     VESPVENKPTSVQFAERQSVDSEDQEHPNLSTKELAKLADKLDREETSTFPELEYFGQ
                     MHGTYLFAQGKTGLCIIDQHAAQERVKYEYYREKIGQVDNSAQQLLVPYIFEFPQNDA
                     LDLVHKMDALRQVGVNLEEYGSNQFILREHPIWMKEEEIESGIYEMCDMLLLTDQVSI
                     KQYRAELAIMMSCKRSIKANHALDDYSARDLLRQLSYCQNPYNCPHGRPVLVHFSKSD
                     MEKMFRRIQENHTSLRELGKY"
     gene            8233..8823
                     /gene="ruvA"
                     /locus_tag="VCA20_001498"
     CDS             8233..8823
                     /gene="ruvA"
                     /locus_tag="VCA20_001498"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEP1835306.1"
                     /translation="MYDYIKGILTNITTKYIVVETHGVGYILQVANPYAYSGQVQQEV
                     TVYTHQVIREDAHLLYGFATENEKSVFLSLISVSGIGPTTALAIIAVDDNDGLVRAIK
                     QKNITYLTKFPKIGKKTAQQMILDLEGKFVMSEEAGPVQQVAPSSENIALEEAMEAME
                     ALGYRPAELKKIKKFFDGTNDTAENYIKSALKMLMK"
     gene            9453..10022
                     /locus_tag="VCA20_001499"
     CDS             9453..10022
                     /locus_tag="VCA20_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEP1835307.1"
                     /translation="MSRCAWVNPNNPLYIAYHDEEWGKPLHDEQSLFELLCLESYQAG
                     LSWEIVLNKRQAFRSAFFNYDIQKVAAMTDSELDSLLTNPDIIRHKAKLYATRANAQT
                     FLRVQEEFGMFDTYLWEWVNFTSIDNPVKSFRELPTKNDLSERVSKDLKKRGFKFVGP
                     ICVYSYLQAAGLLNEHEETCEIGKKLRTN"
     gene            10059..11218
                     /locus_tag="VCA20_001500"
                     /pseudo
     CDS             10059..11218
                     /locus_tag="VCA20_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194467.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
     gene            11270..12421
                     /gene="recA"
                     /locus_tag="VCA20_001501"
     CDS             11270..12421
                     /gene="recA"
                     /locus_tag="VCA20_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEP1835308.1"
                     /translation="MAKKPTKKLEDITKKFGDERKKALDDALKSIEKDFGKGAVMRLG
                     ERAEQKVQVMSSGSLSIDIALGAGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKDGG
                     IAAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSV
                     AALVPRAEIDGDIGDSHVGLQARMMSQAMRKLGASINKTKTVAIFINQLREKVGVMFG
                     NPETTPGGRALKFYASVRMDVRGNTQIKGTGDKKDQNVGKETKVKIVKNKVAPPFKEA
                     VVEIMYGEGISRTGELIEIGSNLGIIQKAGAWYSYNGEKIGQGSENAKKFLADNPAIF
                     DEIDRKIRVHYGLIEADGVEEVATEEAPVVAEEIQDVILDLDGGIELED"
BASE COUNT         3622 a         2388 c         2907 g         3600 t
ORIGIN      
        1 atgtgggctt atagcccttg ttcaaaccac ccgtttgacg ggtggtcgtg attttgtgct
       61 ataatggttt catgacaaat cgaattttag acatggaaca aatgcaggaa gaggagtatg
      121 ttgagcgtac cctgcgtccg cagaaattaa atgaatacat cggtcaggac aaggttaagg
      181 accagctcaa aatctttatc gaggcagcca agctccgtga tgaagccttg gaccataccc
      241 ttctgtttgg acctccaggt ttggggaaga ccaccatggc ctttgtcatc gccaacgaac
      301 tgggcgttaa tatcaagcag actagtggtc ctgttattga aaaagcaggt gacttggtgg
      361 cccttctcaa cgacttggag cctggcgacg tcctctttat cgatgaaatc caccgtatgc
      421 ccatggcggt cgaggagatt ctttactcag ccatggaaga cttctacatt gacatcatga
      481 ttggatctgg agaagctaat cgctcagtac acctggactt accacccttt actttgattg
      541 gtgctaccac acgtgcaggg atgttatcca atcctctgcg ggcccgattt ggtattaccg
      601 gtcacatgga gtattacgaa ctggctgatt tgacggagat tgtcgagcgg actgcggaca
      661 tctttgacat ggagattact catgaagctg ctattgagct ggctcgtcgg tctcgtggaa
      721 ctccccgtat cgccaaccgt ctgctcaagc gggtgcggga tttcgcacag attatgggcg
      781 acggcctgat tgacgatagc attacggata aggccctgac tatgctggat gtggaccgag
      841 aggggctgga ctatgtggac cagaagattc tccggaccat gattgagatg tacggtggcg
      901 gtcccgtcgg cctcaacacc ctgtcggtca atattgccga ggagcgtgag acggtggagg
      961 acatgtacga accttacctg attcagcagg gcttcctcat gcggacgcgg acaggtcggg
     1021 tcgcgacagc taaggcttac gagcacttgg gttatcccta tacggaaaaa taatggcaga
     1081 gcagatgaga tgagtaacga ccttgtttcg agttcggtct aaactaggaa tggagataag
     1141 atgacaccga catttatttg ggatttggat ggaacgctct tggattccta tgaagcgatt
     1201 ttggctggta tacaggaaac ctatgagcag tttggccttt cttttgaccg tgaagaagta
     1261 aggaatttca ttcttcgcta ttctgtcaag gatttgctgg tgcgtgatgc ggacaagtac
     1321 ggtctggata gcgatgaact taatcgtgtg cgtgcgactt ccttgaagga gaaaaatacg
     1381 cagattcctt tgatggctgg tgcgcgtgaa attctggact ggacagcaga gaaaggaatt
     1441 caaaatttcg tttatacaca caagagtgac aatacttttc aggttttgga ggatttgagt
     1501 gtccgtcaac atttcacaga aatcttgaca agcgattcag gttttgcccg caaaccaagt
     1561 ccagaagctc tgctatttct cattgaaaaa tacggactga acaaagaaaa tacctactat
     1621 atcggtgacc gtttgctcga tgtagaaacg gctgtcaacg cagggattca tagcatcaac
     1681 ctgcaaattg atggtttgga gaagaattgg aagattgtgt ctttattgga cataaaacaa
     1741 atattaacag ataaatcaag tcataacaac ctgtgaaaac gggtggcttg tacaccagct
     1801 ataagctgtt tctctccagc gacgtctaaa gatgtccgct gaacttcgtt caggttagtg
     1861 ctatgattac ttgactaatg cccgtaacat cgggcttttg ttttattatg gtttcaagct
     1921 ataataaaaa tgagtccagc cgtctgtttt aagaagagca tgatatggaa tttgtgtaat
     1981 atatttgata taatttaaaa agtatatttg gaggtgtggt ttatgaacga taaggaattt
     2041 ggacagcgtg tacgtcaatt acgagaatct gctagtatga cacgtgaaca gttttgtgac
     2101 gatgaactgg aactctctgt gcgccaatta actcgtattg aagcaggtac ttccaagccg
     2161 actttttcaa agattcagta tattgcaact cgtttaggta tgggacttta cgagcttatg
     2221 ccagattacg tatccttacc cgaaagatat tccaagctga agtttgatgt gcttcgcacc
     2281 ccaacttatg gtaatgaaga tttggcggaa aagcgagatg ccatgatgac agaaatctat
     2341 gacgattatt atgatgaatt gcctgaggag gagaagatag ttgtagatac cctatgttcc
     2401 ctatttgatg tattggatac agatagccag gaatacggta aagaaattct tgatgattat
     2461 ttacatcaaa gctatcaccg ttccaaacta tctataaacg atttaatgat tttgcgatta
     2521 tttattgaac attgtcagtt ggaggattta tctgttggaa cttccaatta tgctttattt
     2581 acagatttga tagagaaatt gcctcaatca atctatgatg tacattcgga atccttattc
     2641 attgtccgcg atttatttct agctattgtt cgtattttat tttcgaagga gttgtataat
     2701 catattccag tctatataga aaaaatagaa aacatcatgg aattatcaca agattttcag
     2761 aagaagccaa ttctaaatct tgtgaaatgg aaatatgaat taaaggtaca acataatcat
     2821 gaaattgctg agaggtacta taatgaagcg attacatttg ccagcctact aaatcaattt
     2881 cacttgaaag aaaaactgca aatggaatgg gaaaaagata cgcagagtct aaaaagatga
     2941 catttttgtc ctgttgagaa tctagagttt attttatact agattcataa aacaaaagga
     3001 gattatatgt tatgtttcaa ttttttaaat tttttggtgg ttttggtcaa cttggtgatg
     3061 agaactggtg ggtaaaataa ataattgaat actcagtata gagcgtttat tttatgggga
     3121 tttaagtcgt ggagtacgat attttcaaaa agccaacatc cgttaggctt ttttgttata
     3181 ctagacagta tgtaggaggt ggctatgtta gaaaattggt taaacaccaa acaaggtcag
     3241 gtttttcatt acaagacgga aaagattgag tatgccctag aactgctagg gaatccccag
     3301 tttgcagttc cggtcattca tgtggctgga actaatggca agggatcgac cattgccttt
     3361 atgcgccagc tctttcagac tcacggcttg cgtgtcggta gttttgtatc tccccacatg
     3421 gtgagtgtac acgacaggat ttgtattgac agccagccca tttcagacca tgattttcag
     3481 cactatttac agaaagtcta cgacttggag caggaagtcg tcactcgtta tgagcctttt
     3541 cgctattttg aggtcatggt gctcattatg ttcctctatt tccaggacca gcagcttgat
     3601 gtggcactag tagaggtcgg catcggaggg cttttggata cgaccaacgt cgtagcaccg
     3661 gctctcagcg tcatcacctc catcggcatg gaccatcagg atttactagg ctcgacttta
     3721 agggaaatag cagagcagaa agcagggatt atcaaggaaa gcgtgcctgt cgtcttagga
     3781 ccactttctt cagaaaccac agctatctgt cgccacattg cccaagacaa acaagcctct
     3841 gtccaccaat ttggtcagga attcacctat aaagcaggac agttcagcaa tacggacatc
     3901 gacctgtcag aattggtttt gggtctggct ggccatcacc aagaagagaa tgcggccgta
     3961 gccctacaaa cttttctact ctatatgacc aatatccaaa aagacattca atctcagttg
     4021 attcaacaag cccttgccca aaccagctgg cctggtcgtt tggaattggt tgctcaagag
     4081 ccaaaaatct atttggacgg ggcccacaat gtccccgcta tcgaacgctt gattgaattt
     4141 attcaggttc aagaagagcc tgttactatt ctcttttcag cccttcggcg taaggatttt
     4201 caagaaatgc tggaattatt ggaagaaaag tcaccgcata ctccccttgt actaaccagc
     4261 tttgcctatg atggtgcctt gtctgaggaa aaccgacaag gacgagatta tgttgagaat
     4321 tatcagcagt ttatagaaga ctggcaatct agtgagcaag gtattttgat tgtcacaggc
     4381 tctctctact ttatctcaga agttcgtcgg attttaaaaa aatgaatatc tttttgcgaa
     4441 taaataggta tgacacatgc agaaaaaata gcatttctaa aggaatttga aaagctcaac
     4501 cgccatacct ttcgacagtt agaactgttt gcaaaaattt ccagaactcc cgataggaaa
     4561 tgacatcggg agtgcttttt gatataataa ggaaagcaaa tccaaggaga agaaaatgac
     4621 agaattagat gtagtttcgg acgtagttac tcatcctgag gaaacttatc ggcgtagtcg
     4681 tagagcatca cgcagacagc aagtgcaact gaatggtgaa tttcatgaaa gagtattaaa
     4741 gacaaagtat tggccagctc ttgtttggag cttagtttta agtattttta gcgtagccaa
     4801 tccactgcta atgccgtttg caaccaatat acaaactcaa aatttatatg cagggatggc
     4861 aatggcaaat ggacaaatcc cttatggtga tttctttggt acaagcgggt tattattcta
     4921 cttacttgct ttcttaggtc atctgggagg gactttcatt atctttggaa tcctccagtt
     4981 tatagcatta ctgatagcgg gagtctattt ctataagatt gtagcttatt ttagtcagtc
     5041 agaacaccta gcgacaagtt ctagtcattg gttctatgta tttatttttg cacttggttt
     5101 tggaggtatg tatgcagaaa tgtttgctct ttcgtttctt ttgactagtg tgtggtttct
     5161 ggtccgctat tttgaaaatg cagttcgtga tgaggcattt atcctctacg gaatcgatgc
     5221 ggcattggta tttttaattt acccaaaaag tctgatcctt tggttagttg caggtctagt
     5281 tttatttgtt tttaatatcc aacaccgcca agttactcga ggaatctatc aattattagc
     5341 gaccattttt ggttttctcc tgattctata tgcggtaggt tattatgctt ttgaggcgca
     5401 gattctgggg acagctattc aacaaacttt cctttataac ttacagttag actttcacca
     5461 ttcctatcta tatttagcct tggcgattgt gtctgtattt ttattcttat ctggattctt
     5521 taaaagtttc atccaaatgg tgttttcttt caaacaagga agacatacct atatcaaagt
     5581 tctcctatta ttgactttct tagttcaatg tgtctttatt gttgggaatg caaactttca
     5641 atggagccaa ctcattcttc ttttaccgta tggttttgcg atgtcagtcg tttacctgcg
     5701 tgatgaggat gtagaagatt atcgtgggta tttgcgtcgt caattctttc ttccgttagc
     5761 tatttgcttg ggtattatcg ctcaacctgc ttatctttat ttggtacagg gagatttgag
     5821 aactgaccgt gaacaagttg ccaattacat tggtgaacaa acgaaagatt cggataagat
     5881 ttatgtttgg gataatagcg caagcatcta tctttcaagt caacgtttat ctgccgcaac
     5941 gattactaca gcagaaccct atctgaatac tgacgacaat aaaaatagct tgatgtatga
     6001 catcaataaa aatgaggcga aatttgtagt cgtcaataag actttaccaa ttctagatga
     6061 aatcaagaca aatttagaat cacagtacca gtctgttcag acaacggatt actttacgat
     6121 ttatcaacag aatgaatgat tgctcaggca gtcatttttt ctttagccta tttgggaaaa
     6181 ggagggaaag tggatatgtg gtataatgga aaaataagca ttcgggaaaa attattaaag
     6241 aaatgtagga gaaatgatgt cacatattat tgaactacca gaaatactag ccaaccagat
     6301 tgcggctggt gaggtcattg agcgaccagc cagtgtggtt aaggagttgg tggaaaattc
     6361 cattgacgca ggggctagtc agattgaaat tagtgttgaa gaggctggcc ttaaaacgat
     6421 tcaaattacg gataacggtg aggggattgc ccctgatgaa gtagcgcttg ccctccgccg
     6481 tcacgccacc agtaagataa aaagtcaatc ggatttgttt cgtattcgca ccctcggttt
     6541 tcgtggagaa gctctgcctt ccattgcttc tgtcagtcat atgatcattg agacagctac
     6601 ggcagactct gcacacggac ttcatttaga ggccaaaggt ggtgtcatcg agaaagagga
     6661 gccagttagt cgtccagttg gtacgcagat tactgtttcg gatttatttt acaatacccc
     6721 tgctcgtctc aaatacgttc gcagtcaaca ggctgaatta tcacatattg ttgatgttgt
     6781 aaatcgattg agtcttgctc atccggaaat agcttttgcc ttagtaaacg aaggacgaga
     6841 attaattaga actgcaggga cagggaaact tcgtcaggca atttcaggga tttatgggat
     6901 tgcttccgcc aaaaaaatgg ttgaaattga ggcagaagat ctggattttc agatttcagg
     6961 ttatgtttcc ttgccagagt tgactcgtgc caaccgtaac tacatttcca ttttcatcaa
     7021 cggtcgctat atcaagaatt ttttgctgaa tcgagcgatt ttggaaggtt acggcagtaa
     7081 gttgatggtt ggacgctttc cgttggcagt gatttctata gaaattgatc cctatcttgc
     7141 tgatgtcaat gtgcatccga ccaagcagga agttcgcatc tccaaggaaa aagaactcat
     7201 gaccttgatt cgagaggcga ttagtcaagc gcttaaagag caggatttga taccagatgc
     7261 tcttgagaat ttagctcagt caagtactcg accaaaagta aaagcagagc aaggcacctt
     7321 accactcaga gagccaaaaa tctattatga tacgattaag caagacttct tcttaaaacc
     7381 agacgtggtc gctgaggatg tcaaacctct cgaagaggat cggcaagaga ttgttgagtc
     7441 gcctgtcgaa aataaaccta catctgttca atttgcagaa cgtcagtcgg tggattcaga
     7501 agatcaggag catcctaatc taagtacaaa agaattggca aaactggcag ataaattaga
     7561 ccgggaggaa acatcgacat ttccagagtt agaatatttt ggtcaaatgc atgggacgta
     7621 tttatttgcg caaggcaaga caggactttg tatcattgac cagcatgccg cacaagagag
     7681 ggtcaaatac gaatactacc gtgagaaaat tggacaggtt gacaattcag ctcaacagtt
     7741 attggtgccc tatatttttg aatttccgca gaatgatgcc cttgacctcg tccacaaaat
     7801 ggatgccctt cgtcaagttg gtgtcaactt ggaagaatat ggatctaatc aatttattct
     7861 gcgtgaacat cctatttgga tgaaggaaga ggagattgag tctggcattt atgagatgtg
     7921 cgacatgttg ctcttgacgg atcaggtgtc aatcaagcag tatcgagcag aactggctat
     7981 catgatgtct tgtaaacggt caatcaaggc caaccatgct ttggatgatt attcggcgcg
     8041 tgatttactg agacaattgt cttactgcca aaatccctat aactgcccgc acggtcgtcc
     8101 agttttagta cattttagca agtcagatat ggaaaagatg ttccgtcgca ttcaggaaaa
     8161 tcacacgagt ttgcgagaat tgggcaagta ttaacatagt tgaaaaagtt gacacattag
     8221 taaaggaagt atatgtacga ctatattaaa ggaattttaa caaacataac tacaaaatat
     8281 attgtggtag aaacgcatgg agtaggctat atcttgcagg ttgctaatcc ctacgcctat
     8341 tcaggacaag tccagcaaga agtgacggtc tatacgcatc aggtgattcg tgaagatgct
     8401 catttgctct acggatttgc tacagaaaat gaaaaatccg tctttctgag tctgatttca
     8461 gtatcaggta ttggtccaac aacggctctg gctattattg ctgttgatga taatgatggc
     8521 cttgttcgtg ctatcaagca gaaaaacatt acctacctga ccaagtttcc gaagattggc
     8581 aagaaaacag cccagcagat gattttggac ctggaaggca agtttgtcat gagcgaagaa
     8641 gcgggtcctg ttcaacaagt agcaccatcc agtgaaaata tcgccctcga agaagccatg
     8701 gaagccatgg aagcccttgg ttaccgccca gccgaactca agaaaataaa gaaattcttc
     8761 gacggtacca acgacaccgc agaaaactac atcaagtcag cccttaaaat gctgatgaag
     8821 taataggata ctaagacgtt aagcgataca aagcaaaata gaaaagctga cgcagaggct
     8881 ttagcatcta ggaagtcttt atctttttgc aaagtatcgt agccgtgttc aattaacgta
     8941 ccagtatgat ggttaaaaag ttttgttttg ttactcaaag tttgagtcaa catctcagcg
     9001 cagtggttga ttggcagatt tgttcgtgtt tcacacttca aatctgacct aatcaactgt
     9061 gcgggggaag gaagacgaat tcttttttat ctgtagaatt ctttcctgct cccactacgg
     9121 caatcgctat ttgcgacttg cctaggtatc ttactaactc gccacaaaaa taatgtcgat
     9181 ttttgtggct catgtcgtgt ccagatacaa aggacgtaaa ggacaaagag aaaatagacg
     9241 gccgaacaga aattttgcta gaaatttcgt tgtgagacct ccgcaatgac aactaggtaa
     9301 aaggcgagtt ttgacgaagc cttttgccag atgtcaactg cgttattcag tgatatgtag
     9361 gattcagttt gacaattttg caaagtatcg tagccgtgtt tcagtcagtt tatcctgatt
     9421 cgcactttag ttattcacga aaaggagttt ctatgtcccg ttgtgcttgg gtcaatccca
     9481 acaatccact ctatattgct taccatgatg aagaatgggg caagccactt cacgatgagc
     9541 agtctttgtt tgagttgctt tgcttggaat cctaccaagc aggactttcc tgggaaattg
     9601 ttctcaataa acgccaggct tttcgctctg cttttttcaa ctatgatatt caaaaggttg
     9661 cggccatgac agatagcgag ttagatagcc tgctgaccaa tccagacatc attcgtcata
     9721 aggcaaagct gtatgctacc cgcgccaatg ctcagacctt tcttcgagta caggaggaat
     9781 tcggtatgtt tgatacgtat ctttgggaat gggttaactt tacatctatc gacaaccctg
     9841 tcaagtcctt ccgagaattg ccgaccaaga acgacttgtc tgagcgggtt tcaaaagatt
     9901 tgaagaaacg aggttttaaa tttgtaggtc ctatctgtgt ctattcttat ttgcaggctg
     9961 caggtctgtt gaatgaacat gaagaaactt gcgagattgg aaagaaatta cgaactaatt
    10021 agatgagcga gcaatcgcac cctagacaga ggaggaatat gaaagcagaa ctaatcgccg
    10081 ttgggacgga gattttgaca ggtcaaatcg ttaataccaa tgctcagttc ctttcggaga
    10141 aatgtgcaga gctgggaatt gatgtttatt tccacacagc tgttggagat aatgaaaaca
    10201 ggcttctgtc tgtactggaa gtagccagtc agcggagcga cttggtccaa ctgaggatga
    10261 cttgaccaag cagaccctgg cgaccttctt aggtagaaat cttgtgtttg atgagttggc
    10321 tatggcaaaa ttagaccgtt tctttgccag tcgcccaggt cgtgtccgta caccaaataa
    10381 tgaacgtcag gcacagattg tggagggaag tcaggcgcta cagaatccag ctggtttagc
    10441 tgttggtggt atggttgagc aagatggtgt aacctatatt gttttgcctg gcccaccaag
    10501 cgagctcaag gccatgtttt ctgagagtct cttaccttta ctgtcccaat ctcagcagca
    10561 actttattcg cgtgtcctac gcttttttgg aattggcgaa agccagttag tgactgtttt
    10621 ggcggacttg atcgacaagc agacagaccc aactcttgct ccttatgcaa aagttggaga
    10681 ggtgacttta cgcttgtcca ccaaagcgac cagccaagaa gaggcaaatc tacgtttgaa
    10741 tcagttggaa gaagaaatct tacaacatga caaactggca gactatttct atgcctacgg
    10801 agaggacaat agtttggtca aaacggtagc gactcgtttg gcggagaaaa gacaaatcat
    10861 cgctatcgtc gaacagggga caggtggtct cttgcaagct gaattgagcc tggctttggc
    10921 tgatcagccg tattttagcg gaggaaaagt catcggtcag ctagggacag aatcgggcag
    10981 gctatcagag gaagctgact gcattcggca ggagctgcaa gctgatttgg gtttggctgt
    11041 gtctgtgctt atcaaaccgg aatcaacaga ggacaacgta cttgcaaaag tatatctcac
    11101 tttggctacg acctcgggta tttcccaaaa agagatagat ttaggtggtt attcgtggca
    11161 ataccttcgc cagcttgctt gtctgcaggc ctgggatttt gtacgaaaca ctttgtgaaa
    11221 tagcagatat ttgctataat agtttgacta agaaaagagg agaaaatcat tggctaagaa
    11281 accaactaag aaattagaag atattacaaa gaaatttggc gatgagcgta aaaaagcgct
    11341 ggatgacgcc ctaaaatcaa ttgaaaaaga ttttggtaag ggtgctgtta tgcgtcttgg
    11401 tgagcgtgct gaacaaaaag ttcaagtcat gagctcaggt agtttgtcaa tcgacattgc
    11461 gcttggagcg ggtggctatc cgaaaggtcg tatcattgag atttacggtc cagagagctc
    11521 aggtaagaca actgttgctc tacacgcagt agctcaagcc cagaaagatg gtggtattgc
    11581 tgcctttatc gatgcggagc atgccttgga tccagcctat gcagcagctc ttggtgtcaa
    11641 tattgatgag ttactcttgt cccagccaga ctcaggggaa caaggtcttg aaattgcagg
    11701 caagctgatc gactcaggtg cggttgactt ggttgttgtt gactcggttg cagcccttgt
    11761 accgcgtgca gaaattgatg gcgatatcgg tgatagccac gttggtttgc aggctcgtat
    11821 gatgagtcag gccatgcgca aactcggagc atccatcaac aaaactaaga cagtggctat
    11881 ttttatcaac caattacgtg aaaaagtcgg tgtcatgttt ggtaaccctg aaacaacacc
    11941 tggtggtcgt gcgcttaaat tctatgcatc tgtccgtatg gatgttcgtg gaaacacaca
    12001 aatcaaaggg acaggcgaca agaaagatca aaacgttggt aaggaaacca aggtgaagat
    12061 tgtgaagaac aaggtggcgc caccatttaa agaagctgtt gttgaaatca tgtacggtga
    12121 aggaatttct cgcacaggtg aattgattga gattggtagc aacctcggca tcatccaaaa
    12181 agcaggtgct tggtattcat ataatggaga aaaaattggc caaggttctg aaaatgctaa
    12241 gaaattcttg gcagataatc cagcaatctt tgatgaaatt gaccgcaaga ttcgtgttca
    12301 ttatggcttg attgaagcag atggagttga agaagttgcg accgaagaag ctcctgttgt
    12361 tgcggaagaa atccaagatg ttatcctaga tcttgatggc ggtattgaat tagaagatta
    12421 attcctaaca aaatagattt aatattgaac tattggtgcg tatctgttat aggtatgtac
    12481 gaagttgttt agtataagaa cgaaaaagtc tggagat
//
LOCUS       DATUCG010000070        12475 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.071, whole genome shotgun sequence.
ACCESSION   DATUCG010000070 DATUCG010000000
VERSION     DATUCG010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12475
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.071"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(29..1483)
                     /locus_tag="VCA20_001502"
     CDS             complement(29..1483)
                     /locus_tag="VCA20_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835309.1"
                     /translation="MGEKNSHHLPLDEEKVLDFEVAKDLTIEEAVKKHKEIEAGVTED
                     DGLLDRYIKQHRAEIESQKFETKINHLPLVEVADEEKNQGHESAEEVEANESSLTEVS
                     EEIAPIVEELSVTPMETLEETVIASTVAMEGLSSVADDSSLELEEDETGDLDHSEGAD
                     RDQKKKFYFWSAVGLSMIGVMATALVWMNSVNKSNTATSSSSTSTSQTSSTASSSTDA
                     NVTAFEQLYNSFFTDSSLTKLKNSEFGKLAELKVLLEKLDKNSDSYTKAKEQYDHLEK
                     AIAAIQAINGQFDKEVVVNGEIDTTATVKSGESLSATTTGISAVDSLLASVVNFGRSQ
                     QEVASATVASEAAVTSSQGADETVSTGVPATTEVASTTVSGSTTDFGIAVPAGVVLQR
                     DRSRVPYNQAMIDDVNNEAWNFNPGILENIVTISQQRGYITGNQYILEKVNIINGNGY
                     YNMFKPDGTYLFSINCKTGYFVGNGAGHSDALDY"
     gene            complement(1493..2659)
                     /locus_tag="VCA20_001503"
     CDS             complement(1493..2659)
                     /locus_tag="VCA20_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEP1835310.1"
                     /translation="MKKQFELIATVAAGLEAVVGREIRNLGYECQVENGRVRFQGDVK
                     SIIETNIWLRSADRIKIIVGQFPAKTFEELFQGVFNLDWENYLPLGCKFPISKAKCVK
                     SKLHNEPSVQAISKKAVVKKLQKHFSRPEGVPLQEMGAEFKIEVSILKDVATVMIDTT
                     GSSLFKRGYRVEKGGAPIKENMAAAILQLSNWYPDKPLIDPTCGSGTFCIEAAMLAKN
                     IAPGLKRSFAFEEWPWVEDQLVVAFRNEAQASIKTDLVLDIAGSDIDARMIEIAKKNA
                     FAAGVEQDIVFKQMRVQDLRTDKINGVIISNPPYGERLLDDEAIVTLYREMGETFEPL
                     KTWSKFILTSDELFETRFGQQADKKRKLYNGTLKVDLYQFFGQRVKRQVQEVQG"
     gene            complement(2713..3077)
                     /gene="rnpB"
                     /locus_tag="VCA20_001504"
     ncRNA           complement(2713..3077)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VCA20_001504"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            complement(3216..3551)
                     /gene="gpsB"
                     /locus_tag="VCA20_001505"
     CDS             complement(3216..3551)
                     /gene="gpsB"
                     /locus_tag="VCA20_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEP1835311.1"
                     /translation="MASIKFTTKDIFEQDFKIGFRGYDQDEVNDFLDDIMKDYDAYEA
                     IIKELKGEIARLKAQAANSPKTTLPTEESNDVLRTERPSSATNFDILRRLNRLEKEVF
                     GKQIVQDQE"
     gene            complement(3622..4161)
                     /locus_tag="VCA20_001506"
     CDS             complement(3622..4161)
                     /locus_tag="VCA20_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEP1835312.1"
                     /translation="MDTKSLLVTGYRHTDLGIFSEKDPRLHIIKSAIRRNFIRFLEEG
                     VSWFILTGQLGFEYWSLEVLEDLRAEGYQLSIATIFPFENHGEQWNEANQAKLARFKQ
                     VDFVKYAYPRYEKPGQFRDYNQFLLDNTAGCYLFYDSENETNLKYLYHMVLKKEGYNR
                     KTLTFEELNEEAENFSNSE"
     gene            4227..4844
                     /gene="recU"
                     /locus_tag="VCA20_001507"
     CDS             4227..4844
                     /gene="recU"
                     /locus_tag="VCA20_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEP1835313.1"
                     /translation="MVNYPHKVSKKINRTSPISSQRVNFANRGMSFEAAINDSNQYYL
                     AHGIAVIHKKPTPVQIVKVDYPKRSRAKIVEAYFRQASTTDYSGVFKRHYIDFEAKET
                     RQKASMPMKNFHAHQIEHMKQVVKQGGICFVLLHFSTLKETYLLPATHLIEFYQVDMG
                     SKSMPLTFIRQYGFEIQMGRFPSIPYLEIVEKNLLGGESFENYNN"
     gene            4813..6999
                     /locus_tag="VCA20_001508"
     CDS             4813..6999
                     /locus_tag="VCA20_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEP1835314.1"
                     /translation="MVNLLKTTTIKKALLITANILLAGAIIACLAGAALVVYYIQSAP
                     ELTEESLTATVSSKIYDKNGNLIADLGAEKRSSAKTEEIPTDLVNAIVAIEDQRFFNH
                     RGVDVIRIAGSLINNLSGGHLQGGSTLDQQFIKLTYFSTSVEDQNLKRKIQEAWLATQ
                     LERRNTKQEILTYYINKVYMSNGNYGMKTAALAFYGKELKDLTLPQLALLAGMPQAPN
                     QYDPYTNPEDAKARRDLVLAEMLEEKYIDNTQYEQAVLTPVTDGLQPLSNAAAYPAYM
                     DNYLKQVVEEVEAKTGYNLLTTGMDVYTNVDPAAQQQLWNIYNTDMYVSYPDDLLQVA
                     STVVDVSNGKVVAQLGGRKQETNVSFGTNQAVETNRDFGSTMKPITDYAPAFENGIYT
                     STADLILDGPYNYPGTPTPVNNWDKQYYGNISVKTAIQYSRNVTAVKALEATGLENAL
                     KFLNSVGINYPDIHYSNAISSNTSDTSSKYGASSEKMAAAYAAFANGGTYYAPQYVNK
                     IVFSDGTVTEYAPKGTKVMSAETAYMMTDMMKAVMSYGYGLNASVSGVPMAGKTGTSN
                     YTDSETDTILASIPEANYSYMVVPDENFVGYSSQYAMAVWTGYTNRMTPILDNSMRIA
                     TDVYHNMMLFMHSDYTATDWEMPSGLVRYGSNYYLRGSRSLSNAYNTYTSSSTSSSSS
                     TETSETTEATTTSSETSSGTSTETATSSTETATTGNNPATGQTDGQ"
     gene            7058..7771
                     /gene="rsmG"
                     /locus_tag="VCA20_001509"
     CDS             7058..7771
                     /gene="rsmG"
                     /locus_tag="VCA20_001509"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEP1835315.1"
                     /translation="MKSEVFYKTLADQGIQLTDQQKHQFHRYFQLLVEWNEKINLTAI
                     TEESEVYLKHFYDSIAPILQGHIQNEPLRLLDIGSGAGFPSLPMKIIFPQLDVTIIDS
                     LNKRINFLHLLAEELELEGVHFYHGRAEDFAQDKNFRAQFDLVTARAVARMQILSELT
                     IPYLKLNGKLIALKASSAEDELTQAKNALNLLFAKVIENHDYTLPNGDPRTLTIVEKK
                     KETPNKFPRKAGMPNKRPL"
     gene            complement(8078..8281)
                     /locus_tag="VCA20_001510"
     CDS             complement(8078..8281)
                     /locus_tag="VCA20_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEP1835316.1"
                     /translation="MVQGTVKWFNAEKGFGFIAQENGPDVFAHFSEIQSNGFKSLEDG
                     QKVTFEVEQGQRGLQATNITKIG"
     gene            complement(8441..9274)
                     /locus_tag="VCA20_001511"
     CDS             complement(8441..9274)
                     /locus_tag="VCA20_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEP1835317.1"
                     /translation="MSQQKLIGYHPGTGLIHSLSAVSKLLFFLIVSILAMITYDTRLI
                     LFIAVFSLALFKMSGIRYKEISLVLILTIIFAAMNALMVHLFAPRYGVELYGADTPLL
                     SGLGVYSLTSQQAFYLVNLLLKYFCTVPLAIIFLMTTHPSQFASSLNQIGVSYKVAYA
                     VSLTMRYIPDIQEEFYTIRMSQEARGLELSRKGKLMDRIKGNLSLVIPLIFSSLERID
                     TISTAMELRRFGKNKKRTWYTQQPLQRIDYAVLLFILALVVVTIYLFFVNQGRFYNPW
                     R"
     gene            complement(9271..10944)
                     /locus_tag="VCA20_001512"
     CDS             complement(9271..10944)
                     /locus_tag="VCA20_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1835318.1"
                     /translation="MKKIIEFKDFTFKYQAQQEPTLKDIQLTIYQGEKVLIIGSSGSG
                     KSTIGQCLNGIIPNIYKGEHSGSLLLDGQEAFNLSVYDKSLLVSTVLQDTDGQFIGLS
                     VAEDLAFALENDMESQVVMKQKVGDWAERLSLTDLLAHRPQDLSGGQKQRVSLGGVLI
                     DESPILLFDEPLANLDPKSGQDTIDLIDRLHKQEGTTTIIIEHRLEDVLYRQVDRVVL
                     INDGRILFNGGPDDLLRTSLLQENGIREPLYLSTLRALGYPIAQAEHLSSVWEIDIEA
                     LNIASLPTLHFENGEKEALLEVKHLHFSYPNKQVLTDIQLTIHKGERLAIVGKNGAGK
                     STLAKVLCGFIQGEGEILWKGQSIKEDSVKERAERIGYVLQNPNQMISQTMIFDEVAL
                     GLRMRGVTEEEIEHRVHHILKVCGLYPFRNWPISALSYGQKKRVTIASILVLNPEIIL
                     LDEPTAGQDQRHYREMMEFLDQLNAQGHTIVMITHDMQLMLDYSDRAVVVVDGQIIED
                     ASPAEILSDDTVIERANLKETSIFHLAERVGVNPLELTTFYMQEERRRR"
     gene            complement(11056..11373)
                     /locus_tag="VCA20_001513"
     CDS             complement(11056..11373)
                     /locus_tag="VCA20_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEP1835319.1"
                     /translation="MAGRFYEAIGFAVATFGDIKSATLPEGGNLILMQADSFAQRLPF
                     QVVASGNEVLISLNVASREEVDRIIERVEANSGQITGCPTDARGFYGASFTDLDGIIL
                     MRL"
     gene            complement(11596..12459)
                     /locus_tag="VCA20_001514"
     CDS             complement(11596..12459)
                     /locus_tag="VCA20_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HEP1835320.1"
                     /translation="MSHLQYTAKSHHLQWNVCQLSQICHHFYQNYCPDSFKHRRNVGL
                     AKVSDESLLVLLLLQAELGITSQRHFYKICHLFPCGQLLERSRFNRRSKQLIWLVQFI
                     RKAMSEMLPSDKLAIIDSFPLSLCQSVRNHRASIFKGLADIGYNASKHLWFYGFKVHM
                     LVTLSGFILNYVVTPASVHDIKVVYELLEGCKQSVILGDLGYLSSELKKDLEQEGYHL
                     WTPFRKNMTGSEEHNNWKVMAMRRTIETRFSELCRLFDIEHTLTRSLAGLQLRMEQII
                     LAHNLRYFEMN"
BASE COUNT         3766 a         2773 c         2223 g         3713 t
ORIGIN      
        1 tgagcttttt gcccagcctc ttttttgttt aataatccaa ggcatcagaa tgaccagcac
       61 cattgccgac aaagtaacct gtcttacaat ttatagagaa taagtaggtc ccatctggtt
      121 taaacatatt gtagtagcca ttgccgttga taatattaac tttttccaag atatattgat
      181 tgcctgttat atacccacgt tgttgcgaga tagtcacaat attttcaaga atacctggat
      241 tgaaattcca tgcttcattg tttacatcat caatcatcgc ttgattataa ggaactcgac
      301 tgcggtcacg ttgcaagacg acaccagctg gcacggcaat accaaagtcg gttgttgagc
      361 ctgaaaccgt tgttgaagcg acttctgttg tcgctggtac accagtagat acagtttcat
      421 cagcaccttg gctgcttgta actgcagcct cacttgctac cgtagccgaa gcgacttcct
      481 gttgtgaacg accaaaattc acgaccgatg ccaatagact gtcaaccgca ctaatccctg
      541 tcgttgtcgc agaaagagat tctcctgatt taaccgtcgc cgttgtatca atttcaccat
      601 taacaacgac ttctttatca aactgaccat taatggcctg aatggctgca atagcttttt
      661 caagatgatc atactgttct ttagctttgg tataactgtc cgaattctta tccaattttt
      721 ccaacagcac tttcagttct gctaatttac caaactctga attttttagt ttggtcaaac
      781 tactatccgt aaagaaacta ttatagagct gttcaaaagc agtcacattc gcatctgtcg
      841 aacttgatgc tgtcgatgac gtctggcttg tacttgttga acttgaagaa gtcgcagtat
      901 tcgatttgtt aaccgaattc atccaaacca gcgccgtcgc cattacacca atcattgaaa
      961 gccctactgc tgaccagaag taaaatttct ttttctgatc cctatctgcg ccctcactat
     1021 ggtccaagtc tccggtttca tcttcttcca attctaaact agaatcatct gccactgaag
     1081 ataacccctc cattgctacg gtacttgcaa tcactgtttc ttccaaggtt tccattggag
     1141 ttaccgaaag ttcttctaca ataggagcaa tctcctcact aacttccgta agagaagact
     1201 catttgcttc tacctcttcc gcgctttcat gtccttgatt tttttcctca tcagccacct
     1261 caactaaagg aagatgattg attttggttt caaatttttg agattcaatt tctgcgcggt
     1321 gttgcttgat ataacgatcc aagagaccat catcttctgt cacacctgct tcaatctcct
     1381 tatgtttttt gactgcttct tcgattgtta aatctttcgc cacttcaaaa tctaaaactt
     1441 tctcctcatc caaaggaaga tgatgactat ttttttctcc cacgatgtcc tcctatcctt
     1501 gcacttcttg tacttgacgt ttcactcgtt gaccaaaaaa ttgataaaga tccactttta
     1561 gggtaccatt atacagtttg cgtttcttat ccgcttgctg tccaaaacgg gtttcaaaca
     1621 actcatcact agttagaata aacttagacc atgttttgag tggttcaaaa gtctccccca
     1681 tttctcgata cagggtaaca atggcttcat catccaacaa acgttctccg taaggtggat
     1741 tagaaatgat gacaccatta attttatctg tacgcaagtc ttgcacacgc atttgcttga
     1801 aaacaatatc ctgctcaact cctgccgcaa atgcattctt cttcgcaatt tcaatcatac
     1861 gagcatcaat atctgatcct gcaatatcta ataccaaatc tgttttgata gaagcttgcg
     1921 cttcattgcg aaatgcaacc accaattgat cctcaaccca cggccattcc tcaaatgcaa
     1981 aacttctctt caagcctggg gctatatttt tggccaacat agctgcctca atacagaaag
     2041 ttcccgatcc gcaagtagga tcaataagcg gtttatctgg ataccaattc gataattgca
     2101 aaatagcagc tgccatattt tctttgattg gtgcgccacc tttttccacc cgataaccac
     2161 gtttgaacaa actagaacct gtcgtatcaa tcatgacagt cgccacatct ttcaaaatcg
     2221 acacttcaat tttaaactct gcccccatct cttgcaaggg aaccccttcg ggacgagaga
     2281 agtgtttctg cagtttcttt acaactgctt tcttagaaat agcctgcaca cttggttcat
     2341 tatgcaattt cgatttgaca catttggcct tggaaatagg gaacttgcag cctagaggca
     2401 agtaattttc ccagtccaag ttaaaaactc cttggaacaa ctcttcaaaa gtctttgctg
     2461 gaaattgtcc aacaatgatt ttaattctat ctgctgaacg cagccagata ttggtttcga
     2521 taatggattt tacatctcct tggaatcgaa cacggccatt ttccacttga cattcatagc
     2581 cgagattacg aatttctcga cccacaaccg cttccagacc agctgctacc gttgcaatca
     2641 attcaaattg ttttttcatt tttttcctct acaataaaag ctagcagagc tagctaccta
     2701 ttttatgcaa ttttctgtaa gccatgtttt gttccggtgg tactaggttc taccaccttc
     2761 gataaccatc tgtctactgt ctaaacagtc aggatttctg ttcatttcca tccatcccat
     2821 gccccgacca aagtttgggt tgctagcttg aggggtttac cgcgttccat tttttacgtt
     2881 tccataaaaa cttcgtcact gtggcacttt cagatctact ctggcatatc aaaagactta
     2941 gccattttaa ccgccgtaac gcccaagggc gtccctaggc ttattgattc gcctagcaca
     3001 aacactactg gcatctcagc cagtgctagc atggactttc ctcatggtag aaaactacca
     3061 cgcgattatc caaaaattgc accttaatta tcttagaact tgtatctaca aagcaagcca
     3121 ctttaaatca aggaatatct tgtacacaaa tacagaatgt atttgtgtac aagaaaataa
     3181 ctgcgaataa agtgtcagtt taggtgtaca attttctatt cttgatcctg aacgatttgt
     3241 tttccaaata cttctttctc cagacgattc agacggcgga gaatatcaaa gtttgtcgct
     3301 gaagatggac gttccgtacg caatacgtcg ttcgattctt cagtaggaag agttgttttg
     3361 ggagaatttg ctgcctgcgc tttcaatcga gcaatttcgc cttttaattc cttgataatc
     3421 gcttcataag catcatagtc tttcataata tcatcaagaa aatcgttcac ttcatcttga
     3481 tcatagccac gaaaaccaat tttaaaatct tgctcgaaaa tatcttttgt cgtaaattta
     3541 atacttgcca tctctctctc cctaatctaa aacgtcacta ctcctattat attaaaaaaa
     3601 atagggaaaa atcaagtcaa atcactcaga atttgaaaaa ttttcagcct cctcattcaa
     3661 ttcttcaaag gtaagcgttt ttctattata tccttctttc tttaagacca tgtgatataa
     3721 atatttcaaa ttggtttcat tttcagaatc gtaaaataaa taacaacctg ccgtattatc
     3781 cagtagaaac tggttatagt ctctaaactg ccctggtttt tcatagcgtg gataggcata
     3841 ctttacaaaa tcaacctgct taaaccgtgc taactttgct tggttagcct cattccactg
     3901 ttcgccatga ttttcaaatg gaaatattgt tgcaatactc agttgatatc cctcagctcg
     3961 taaatcttct aagacttcta aggaccagta ttcaaaaccc aattgacctg tcagtataaa
     4021 ccaactaaca ccctcttcta aaaaacgaat aaaattccta cgaattgcag atttaataat
     4081 gtgcagtcga ggatcttttt ctgaaaaaat tcccaggtct gtatgtctat aacctgttac
     4141 caaaaggctc ttagtatcca tttcttgtac tcttttcgct aattatggta taatatgctg
     4201 atattatttt atcaagaagg agttctatgg tcaattatcc tcataaggtg tcaaaaaaaa
     4261 tcaatagaac cagtcctatt tcatcccagc gagtaaactt tgctaatcgc ggaatgtctt
     4321 ttgaggccgc tatcaacgat agcaatcaat attaccttgc gcacggcatt gctgtcattc
     4381 acaagaagcc aactcccgtg caaattgtaa aagttgatta ccccaaacgg agccgtgcta
     4441 agattgtcga ggcatacttt cgacaggcat ctaccacaga ctactctggt gtttttaaaa
     4501 gacattatat tgattttgaa gcgaaagaga cacgccaaaa agcatctatg cccatgaaaa
     4561 attttcatgc gcatcagatc gaacacatga aacaggttgt taaacaaggt ggtatttgtt
     4621 ttgtccttct tcacttttca acattaaaag aaacctatct cttgccagcc actcatttga
     4681 tcgaatttta tcaagtagac atgggtagta agtcaatgcc tctaaccttt attcgacaat
     4741 acggctttga gattcaaatg gggcgtttcc ccagcatccc atatctggaa atcgttgaaa
     4801 aaaatctatt aggtggtgaa tcttttgaaa actacaacaa ttaaaaaagc tcttctaatc
     4861 actgcaaata ttttactggc gggggctatc attgcctgcc tggccggagc tgcattggtt
     4921 gtttactaca tccaaagcgc tccagaattg acagaagaat ctttgacagc aactgtgtca
     4981 agtaaaattt acgataagaa cggaaatctt attgcagatt taggagcaga aaaacgttcg
     5041 agtgccaaga ctgaagaaat tccaacagac ttggtaaatg cgattgtcgc aatcgaagac
     5101 caacgcttct tcaatcatag aggggttgac gtgattcgta ttgcaggttc tctgattaat
     5161 aacctgagtg gaggacattt gcagggtggc tcgacacttg accagcagtt tatcaagctg
     5221 acttatttct cgacatccgt agaagatcaa aatctaaaac ggaaaattca agaggcttgg
     5281 ctggctactc aattggaacg tcgcaatacc aaacaagaga ttttgaccta ctacatcaac
     5341 aaggtgtata tgtcaaatgg taattacggt atgaagactg ctgctcttgc tttttatgga
     5401 aaagagttaa aagatttgac tcttcctcaa ttagctcttt tagcaggaat gccccaagcg
     5461 ccaaaccaat atgatcctta taccaatcca gaagatgcaa aagcacgtcg cgatttggta
     5521 ttggctgaga tgctggaaga aaagtacatt gataataccc agtatgaaca agctgttcta
     5581 acacctgtaa cagatggttt gcaaccacta tctaatgcag ctgcttatcc tgcttatatg
     5641 gacaactatc tgaaacaagt cgttgaagaa gttgaagcaa aaacaggtta caacttgctg
     5701 acaacaggga tggatgtcta tacgaacgtc gatcctgccg cccagcagca actttggaat
     5761 atctacaata cagatatgta tgtcagttat ccagatgatt tattgcaggt agcttcaacg
     5821 gttgtggatg tttctaatgg taaagtcgtt gcgcaattag gtggtcgtaa acaagaaaca
     5881 aatgtctcat ttggtaccaa ccaagccgtt gaaaccaacc gcgacttcgg ttctacaatg
     5941 aagccaatca cagactatgc tcctgctttt gaaaacggca tttatacatc tacagcagat
     6001 ttaatcttag acggtcctta taactatcct ggtacaccaa ctcctgtcaa taactgggat
     6061 aaacaatact acggtaatat ctctgttaag actgccattc agtattctcg aaatgttacc
     6121 gccgttaaag cattggaagc tacgggctta gaaaatgcct tgaaattttt gaatagcgtt
     6181 ggtatcaatt atccagatat tcattactct aatgctattt caagtaatac atccgatact
     6241 agcagtaagt atggagcaag tagtgaaaaa atggctgctg cctacgctgc ttttgccaat
     6301 ggtggtactt actacgctcc acagtatgtc aataaaattg tctttagtga tggaacagta
     6361 acagaatatg ctcctaaagg gaccaaagtc atgtctgccg aaacagccta catgatgacc
     6421 gacatgatga aggctgttat gtcttacggt tatggattaa acgcttctgt tagtggtgtt
     6481 cctatggccg gtaaaacagg tacatcaaac tataccgata gcgaaacgga tactattcta
     6541 gcttcaattc cagaagcaaa ttatagctac atggtcgttc ctgatgaaaa cttcgttgga
     6601 tattctagtc agtacgcaat ggctgtttgg actggatata ccaatcgtat gactcctatc
     6661 cttgataata gcatgcgaat tgcgacggat gtctaccaca atatgatgct ttttatgcat
     6721 tcggactata cagctaccga ttgggagatg ccaagcggac tcgtacgtta cggttccaac
     6781 tactatctca gaggtagtcg ctcactctcc aatgcttata atacttacac tagctctagt
     6841 acaagttctt ctagttcgac tgaaactagt gaaacaaccg aagcgacgac aacatcaagc
     6901 gaaacatcat ctggaacttc aaccgagact gcaacttctt caactgaaac tgcaacaaca
     6961 ggaaacaatc ctgcgacggg acagacagat ggtcaataag aaaattccca gactcttctg
     7021 ggtttttctt ttacttaaat tatgataaga taagattatg aaatctgaag tattttataa
     7081 aaccttggca gaccaaggta ttcaattaac tgaccaacaa aaacaccaat ttcatcgcta
     7141 tttccaacta ttagtggaat ggaatgaaaa aatcaatctg acagctatta ccgaggaaag
     7201 cgaagtctat ctgaagcatt tctacgattc tatcgctcca atcctgcaag gccatattca
     7261 gaatgaacct cttcggcttt tagatattgg atctggagct ggatttccta gcctacctat
     7321 gaagatcatc tttccgcaac tagatgttac catcattgat tctcttaata aacgaattaa
     7381 ctttttacat ttgcttgccg aagaactaga gctcgaaggt gttcatttct atcatggtcg
     7441 tgcagaagac tttgcacaag ataagaactt ccgtgcacaa tttgaccttg tcacagcacg
     7501 cgcggttgcc cgcatgcaga ttctttctga attgactatt ccttatttga agctcaacgg
     7561 aaaattaatc gcactcaagg cgtccagcgc agaagatgaa ttgacccagg caaaaaacgc
     7621 actcaatctc ctcttcgcaa aagtcatcga aaaccacgac tatactcttc caaatggcga
     7681 ccctcgaaca ttgaccattg tcgaaaagaa aaaggaaact ccaaataagt ttccacgcaa
     7741 agctggtatg ccaaataaaa gaccccttta aacataagaa aagaggctgg gcaaacagct
     7801 caacctcgct tctcagggtt cgtgtcaaca tctcagcgca gtggttgatt ggtagatttg
     7861 ttcgtgtttt acactccaaa tctgaccatt acgactgttg cgaacaaagt tcgcttcatt
     7921 tccaacctcc aacagtcact actctgactg ttggagctat gcgggggtgg gagtgaaaca
     7981 gtctggggat agactgtttc agctcaacaa ctggaaataa ggacttgttg acgaactctt
     8041 tttgacctag tcgagttctt tcccgctccc tcttttatta tccaattttt gtaatattgg
     8101 ttgcttgaag accacgttgg ccttgctcca cttcaaatgt cactttttga ccatcttcca
     8161 aagatttgaa accattagac tgaatttcag aaaagtgtgc aaatacatct ggaccgtttt
     8221 cttgagcaat aaaaccaaat cccttttctg cattaaacca tttaactgta ccttgtacca
     8281 tttgtgttac ctctttttat ttatttgtaa aacttatcaa gaggaaaaac aaaagatgaa
     8341 actgatactt aaactctaaa tttatttaca ttccctactg taacagtata tttcctatat
     8401 gtcaacgata aagggttgat aagcccaata aactcaataa tcatcgccac ggattataga
     8461 atcgaccctg attaacaaaa aatagataga ttgtcacgac tactagagca agaataaata
     8521 gcaagacagc gtaatctatt ctctgtagag gctgttgggt ataccatgtt ctttttttat
     8581 tctttccaaa acgccgcaat tccatagccg ttgaaatcgt atcaatcctc tccaaagagc
     8641 tgaaaatcag aggaatgaca agggatagat tcccttttat acgatccatc aattttcctt
     8701 tcctggacaa ttccaaacca cgcgcttctt gagacattcg aatcgtataa aactcttctt
     8761 gaatatctgg aatataacgc atggtcaaac tgactgcgta agcaactttg taggataccc
     8821 caatttgatt caagctggaa gcaaactgac taggatgagt ggtcatgaga aagataatcg
     8881 ctaaaggaac tgtacaaaaa tacttcaaga gaagattcac cagataaaat gcttgctgac
     8941 tcgtcaagga ataaactccc agacctgata gcaagggagt gtctgctccg taaagttcca
     9001 ctccataccg tggtgcaaac aagtgaacca tcagggcatt cattgcagca aaaatgatcg
     9061 tcagaattaa aacaagagag atttctttat aacgaatccc tgacattttg aacaaggcta
     9121 gagaaaatac tgcgataaaa agaatcaagc gggtatcgta agtaatcata gcaagaatgg
     9181 aaactatgag aaaaaagagt aatttactga cagccgataa actatgaatc aagccagttc
     9241 ctggatggta accaattagt ttttgttgac tcatcttctt ctcctttctt cctgcatata
     9301 aaacgtcgtt aattctaagg gattaactcc caccctctcc gccaagtgaa aaattgatgt
     9361 ttcttttaaa ttggctcgct caatcaccgt atcatcagat aaaatttctg ctggactggc
     9421 atcttcaata atctgcccat ccaccacaac aacagctcta tctgaataat ctagcatgag
     9481 ttgcatgtca tgggtaatca tgacaatggt gtgcccttga gcatttaatt gatccaaaaa
     9541 ctccatcatt tcccgataat gtcgttgatc ttgtcctgct gttggctcat ctagcaaaat
     9601 aatttctgga ttgagaacca agatagatgc tatcgttacc cgttttttct gcccatacga
     9661 aagtgccgaa atcggccaat tacgaaacgg atacagacca cacactttca agatatggtg
     9721 aactcggtgt tcaatttctt cttctgtcac ccctcgcatg cgcaaaccaa gggcaacctc
     9781 atcaaagatc atcgtttggc taatcatttg gttgggattc tgaagaacat aaccaatccg
     9841 ttctgctcgc tctttgacag aatcttcttt gattgactgc cccttccaga gaatctcacc
     9901 ctcaccctga ataaaaccac aaagcacctt tgctaaagta gatttcccag caccattttt
     9961 tccaacaata gctagacgct cccccttatg aatcgtcagt tgaatatctg taagaacttg
    10021 tttgttagga taggaaaaat gaagatgctt gacttctaac aaagcctctt tctctccatt
    10081 ttcaaagtgt agagtcggta gggatgctat attcaaagcc tcaatatcaa tttcccaaac
    10141 tgaggagagg tgttctgcct gcgcaatcgg atatcccaag gcgcgaagag tcgatagata
    10201 taagggttca cggatgccgt tctcctgtaa caatgaggta cgtaataaat catcaggtcc
    10261 tccattgaac aaaatccgac cgtcattaat caatacaact cggtccacct gacgatacag
    10321 tacgtcctct aaacggtgtt cgataataat ggttgtggtc ccttcttgct tgtgcagacg
    10381 gtcaatcaaa tcaatcgtat cttggcccga tttggggtct aaattagcaa gtggctcatc
    10441 aaacaacaaa ataggactct catcaataag aactccgccc agactgactc gttgcttttg
    10501 acctccagat aaatcttgtg gtcgatgtgc tagtaaatcg gtcaaagaca gtctttctgc
    10561 ccaatctcct actttttgct tcatcaccac ctggctttcc atatcgtttt ccaaagcaaa
    10621 agctaggtcc tcggcaactg ataaaccaat gaattgacca tctgtatctt gaagaaccgt
    10681 tgatacgagc aaggacttat cataaactga aagattaaac gcttcctgac catccaatag
    10741 taaacttccg ctatgttctc ccttgtaaat attgggaata ataccattaa gacattgtcc
    10801 aatcgtcgac tttcctgaac cagatgagcc aataatcaga actttctctc cctgataaat
    10861 cgttaactga atatctttta aagtgggttc ctgttgcgct tggtatttaa atgtaaaatc
    10921 tttaaactca atgatttttt tcataggact attatagcgt aaattgactc tgaaaaaaag
    10981 gaagaatcat cagtttctcc cttttcaaat aagttcatcc atgattctag agtagatgaa
    11041 ttctaacttc ctacatcaca atcgcattaa aatgatacca tcaaggtccg taaaacttgc
    11101 tccataaaag ccacgggcat ctgtcgggca tcctgtaatt tgcccgctat ttgcttcaac
    11161 acgctcgata attctatcca cttcttccct actcgccaca ttaagcgaaa tcaagacttc
    11221 attgccactc gctactacct gaaatggtag gcgttgagca aagctatctg cctgcatcaa
    11281 aatcaagttg ccaccctcag gcaaagtcgc agacttaata tctccaaacg tcgcaactgc
    11341 aaaaccgatt gcttcataaa accgaccagc tattcaaggt ctgcttactg ctaatactca
    11401 atgaaaatca aaagtaacct aggaaatgaa atgaagcgaa gatagaactg aagttcatca
    11461 aggcaagttg acaacggata atgttgattt tcgaagggga taagttcatc caaaatgact
    11521 tcatctcctt ctcttttcta attgataggc ttcgtataga atggactgaa aaaatataaa
    11581 gaattacccg tggtgctagt tcatttcaaa atagcgtaag ttatgagcta gaataatttg
    11641 ttccattcgt aactgtagtc cggctaggct tctagttaat gtatgttcga tgtcaaagag
    11701 acgacacagt tcagaaaaac gtgtttcaat agttcgtctc atggccatta ctttccaatt
    11761 gttatgttct tcggacccag tcatgttttt acgaaacggc gtccatagat ggtaaccttc
    11821 ttgctctaag tcctttttga gctcactgct taggtagcct agatcaccta agataaccga
    11881 ttgcttacac ccctctaata gctcataaac caccttgatg tcgtgaacag aggcaggtgt
    11941 gacaacatag ttcagaataa aaccagataa ggtcactagc atatgaacct tgaagccgta
    12001 gaaccaaaga tgtttagagg cgttgtagcc aatatcggct aatcctttaa aaatgctagc
    12061 tctgtgatta cgaacggatt ggcaaagtga caaaggaaaa ctatctataa tagctagttt
    12121 atcagatgga agcatttcac tcatagcttt tcggatgaat tggacgagcc aaatcaattg
    12181 ttttgaccga cgattgaaac gacttctttc tagtaactga ccacaaggaa agaggtggca
    12241 gatcttgtag aaatgacgct gtgacgttat acctagttcg gcctgtaaca agagcaagac
    12301 aaggagtgat tcatctgaaa ctttggctaa gccaacatta cgccgatgtt tgaaggaatc
    12361 tggacaatag ttttggtaga agtgatgaca aatttgtgat aattggcata cattccattg
    12421 caaatgatga gatttagcgg tatactgtaa gtggctcatt tggactaacc tcctg
//
LOCUS       DATUCG010000071        12245 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.072, whole genome shotgun sequence.
ACCESSION   DATUCG010000071 DATUCG010000000
VERSION     DATUCG010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12245
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.072"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            110..1471
                     /locus_tag="VCA20_001515"
     CDS             110..1471
                     /locus_tag="VCA20_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEP1835321.1"
                     /translation="MLKTLKLVTIGGGSSYTPELVEGMILRAHQLPIREWWFVDVEQG
                     AKKQEIVVELARRMVAKAGLDWKIESTLDRREALIEADFVTSQFRVGQLPARYLDETI
                     PLSYGMLGQETNGAGGIFKAFRTIEAYKGIIKDMKDLCPNAWLINFTNPAGIVTEAIA
                     NELHWKKVIGLCNIPIGQKKLAAQILGQSEGGLSTRHIGLNHFHFHEVWDKYGENRTQ
                     VVLETLYGDLREETPEAVKNITNLEFPIQLLRTIGMLPCDYHRYYFLEQEMLEDALEQ
                     YRQGNVRAKVVQEVETELFELYKDPQLKDKPKQLELRGGAYYSDVACQIICAIHNDSG
                     EELTVSTVNRGVIPYLPENCVVEVTSIISAQGATPLACPPTPPIVTGYLQLMKQMELV
                     TVQAAMTGDYSLALQAFTLNPLISNGPQAEVLLQDMLLAHENYLPQFAPAIQHIKERR
                     NNQ"
     gene            1468..2868
                     /locus_tag="VCA20_001516"
     CDS             1468..2868
                     /locus_tag="VCA20_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEP1835322.1"
                     /translation="MTKDKVFYQFPTNFLWGSSTSGPQSEGIEPGDGKGQNNWDYWYS
                     IEPERFHNKIGPSQTSTFYKHYEKDIDLLVETGHTVFRTSIQWSRLIPTGTGEINQEA
                     VSFYRSVFSLVRAKGIQLFVNLYHFDLPYCLQEKGGWESKEVVRAYEAYARTCFELFD
                     DLVDRWITFNEPIVPVECGYLGNYHYPCKVDAKAAVAVAYHTQLASSLAVKACHEVNP
                     NKKIAIVLNLTPAYPRSEQAEDIKAARIAELFQTKSFLDPSVLGKYPDELVELIDAYG
                     LMPEVSDEELAIIKENRVDFLGVNYYQPLRVQAPTNSWKEGEPLTPEHFFAPYDMPGK
                     KMNPHRGWEIYEKGIYDIAINIKENYHNIEWFVTENGMGVEGESAFKDNGLIQDDYRI
                     EFIEDHLIELHRAIQEGANCKGYMLWTFIDCWSWLNAYKNRYGLVELDLDTQERIVKK
                     SGKWFKKLSDANGFHR"
     gene            2878..3984
                     /locus_tag="VCA20_001517"
     CDS             2878..3984
                     /locus_tag="VCA20_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEP1835323.1"
                     /translation="MVELGFSIYPENYSLEESQAYIKLLHQYGARRLFMSLLQLENGD
                     RTVFELYQRLIDYANQLGMTVIADISPHFIAAQGWDGALMERAAELGLAGIRLDEALP
                     LEEIIEMTHNPHNIKVELNLSTDKILLSNLLASVANRDNIIACHNFYPHAFTGLSEEH
                     FLEMSSFYHQSGIRTAAFVSAQTASEGPWPLSEGLPTLEDHRFWPLPLQIAWMKATGF
                     IDSILISNQFILEEELQSIQTVLNEDTISLMVDIEEGLTPVEREIVAFNHVYRGDISA
                     HVLRSTMPRVVYKDASVPARSDQAIPVGRGDILIDNDLYGRYKGELQIALKEFTISPK
                     VNKVGRISPDYLPLLRCIKPWQEFKLVIASEHFH"
     gene            4076..6070
                     /locus_tag="VCA20_001518"
     CDS             4076..6070
                     /locus_tag="VCA20_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEP1835324.1"
                     /translation="MLSKKEVRLLEALLAKQETFVPSIELAKVLSVSDRTVRTYIHQL
                     TSSLENSGAHILSKQGSGYCLQIERPVEFEMFWQEQLLLKKRLTDLTQLEEAGDRQRY
                     ILNKLLFEDPIQSPDCLQRELFIGKTTLHSILADIRKLFIPYQLELLVTRKGIELKGD
                     EPAIRHFIMDYFFDEGNVQSVYGVVETNLLPDIRFTDLMLIVLDECRDAKLRLSDFVM
                     QNLVLHIALLLQRMKIGYGLDQFPISEHIHSSQEYLVAQRIIERIEETFQVTIPSEEA
                     NYIALHLKVKLTGNPNQDGEVEEIFRQNVENSLLHLSHLTGLELSKDQQLFKGVLAHL
                     MPLRIRLENNIQLGNPLTAEIKDQYFEAFELTKIAFAAIPELMGYSISDDEWAYLTLH
                     VMAAIERYQGFKKTRVLVVCATGIGSALMLKNRLEKEFSSSLEILDTVSYYEVTEERL
                     KDVDLIISSISLSNLIFMTPVINVSVFLSEKDIEAIRSHLQQTTPVIPDQGENVLMEV
                     GQAKQIAEAVFAPHRIIYLEEDCDREVALKQLVTTLNESIEPDFVNRFIEQVNLRESY
                     SPVVFGDRLAFPHPALPMSYSEQIVVGIYKNAVDWGHGKQVQFVFLLSPSKGRNAFLK
                     YISPCLVDFVQDRVLQERLLHHPNYKELIDIFIPLIQRKG"
     gene            6083..6394
                     /locus_tag="VCA20_001519"
     CDS             6083..6394
                     /locus_tag="VCA20_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEP1835325.1"
                     /translation="MKHIMLVCNAGMSTSMLVTKMQKAAEETGVEATIWAVPVSEADN
                     EVAQKEIDVLLLGPQVKFLLNDYKAKFEPTIKVDAINMMDYGVMNGAKVLEAALKMME
                     G"
     gene            6396..6710
                     /locus_tag="VCA20_001520"
     CDS             6396..6710
                     /locus_tag="VCA20_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEP1835326.1"
                     /translation="MNSPEYLEAIMGLIMYGGEAKGFASQAIRAAKEGRIDEAEQLLQ
                     DAIASLNIAHKSQTQLLAKEASGESVELSLLMVHGQDHLMNALTFIDLAKEFVDLYKQ
                     IG"
     gene            6729..7169
                     /locus_tag="VCA20_001521"
     CDS             6729..7169
                     /locus_tag="VCA20_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HEP1835327.1"
                     /translation="MKISKIGQVPIERLFATIRQSLKDDFQQNTGSILQDQDIIQGLT
                     YTKTFGDRGQHSVKVTVEEFTAPYFYAVCFHSNRGKEHVRYQLEDLGNAQTNIKYSYE
                     MEAGDIFVKGNQFLMEKIFSKSIKRQTNAQLEALIRCSVEPVSV"
     gene            7213..8589
                     /locus_tag="VCA20_001522"
     CDS             7213..8589
                     /locus_tag="VCA20_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEP1835328.1"
                     /translation="MSNISEKFMEISGKIGSQRHLIAIRDSFISMMPITMAGTVAVLL
                     NVFLRDIPNNMGWTGFADAMQPVIDINGYVYFGTIGIMALVFAFTFGYNLSLMYKVNP
                     LAGGLISFASFISTIPQSVTISTALDGVDKSVVSALEGLGLSIVTTDGVSALETSQWG
                     AIALKYGGATGLFTALFIGFLATWVYASLVKRNVIIKLPDSVPPAVNKAFAAIIPGTA
                     AIYASSIVAYLAFTLTGQSLGDLVSTYVQLPLLGLSQGLGSVILLTFLVQLFWFFGLH
                     GHNVLAPIMDGVYMVALNENAAAYTATHSTANLPWLWTRGSFDAYAQMGGSGVTLALI
                     IAIFIFSKREEYKMVAKLSAPMGVFNINEPVTFGIPMVLNPLFAIPWLIVPPICATIA
                     YLATAAGIIPPVFAPVPWITPPGLYAYLATGGNIMAALVSLFNLFVAFLIWTPFVIAA
                     NKVKAGEE"
     gene            8921..9529
                     /locus_tag="VCA20_001523"
     CDS             8921..9529
                     /locus_tag="VCA20_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEP1835329.1"
                     /translation="MSYQMIKKQFKLRLFTALIGNLLLLGMVNVSADDTVISDSPTVS
                     ALIKKVEAAKIAAKAAAEKIVADKATAAAKAALATNMVSEVAVEYTANTYPAGQCTWG
                     AKEMAPWVGNYWGNGGDWAASAAELGYEVGTTPKVGAIAVWTDGGYGHVAYVTDVAAN
                     GHIQVKESNYCGVYYPSNVRGFFDPTTTSEGTVSYIYPPAGV"
     gene            9871..10674
                     /locus_tag="VCA20_001524"
     CDS             9871..10674
                     /locus_tag="VCA20_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEP1835330.1"
                     /translation="MKKIAMFTSLLFAGIVLTACNSSAGTSNSTDQTLLAQIKEEGVI
                     QIGTEGAYAPYSYHDESGKLVGYDVEVAEAVAEKLGVKVEFVETKWDSMIAGLDAARF
                     DTIANQVGITDERKAKYDFSTPYTYIYGALVIQKDNTDITGFADLSGKKSANSLTSNW
                     ADLARENGAEVVGVDGFSQAVELLNTGRVDATINDNLVYLDYVKQHADAPIKIVTLTD
                     DVSTTAFPVAKGNEDLVKEIDAALAELASEGKLAEISNKYFGEDVSKAK"
     gene            10687..11346
                     /locus_tag="VCA20_001525"
     CDS             10687..11346
                     /locus_tag="VCA20_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEP1835331.1"
                     /translation="MTERVWQLLLDSFSQILVPGLLVTIPLTILSFTFGLLIAIGTAL
                     VQIAQIPILKQVARFYIWVVRGTPLLVQLYVIFFGLPSLGIVLDAFPSAVLVFSVNTG
                     AYAAETIRASIESVPKGQLEAGYSVGMSFAQTMRRIILPQGFRVAFPPLSNTLIGLVK
                     DTSLAANITVLEMFMATQQIAARTYEPFALYCEVALVYLLFSTILTKLQAYGEKKLAV
                     Y"
     gene            11356..12102
                     /locus_tag="VCA20_001526"
     CDS             11356..12102
                     /locus_tag="VCA20_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEP1835332.1"
                     /translation="MIQASGVKLTFGGQQVLEGIDVEVNPGEVVVILGPSGSGKTTLL
                     RSLNFLEKADAGHLVFGEDSYDLAQMTKKEIAGIRKRTAFVFQNYNLFANKTALQNVT
                     EGLIVARRVPKQEAERIGKAALDKVGLSDKYGFYPSQLSGGQQQRVGIARAIATNPEV
                     IYFDEPTSALDPELIGEVLAVMMQLAKEGMTMVVVTHEMGFARAVADRVIFMDGGRII
                     EEDRPEDIFDQPKEERTKQFLARIQKVDVE"
BASE COUNT         3654 a         2141 c         2789 g         3661 t
ORIGIN      
        1 tcatttcgtg gttgttttga gtttatgata cgcaaagtgt ttttatttag ctagaattaa
       61 ctagcacaac gggtaataag aatataaaag aagatgaggg agggaaagcg tgttgaaaac
      121 tcttaaatta gtgacaattg gtggaggctc aagttacact ccagaattgg ttgaaggtat
      181 gattcttcgt gcacatcaat tacctattcg agaatggtgg tttgttgatg ttgaacaagg
      241 cgcaaaaaaa caggagatcg tagttgagtt agcacggcga atggtcgcaa aagcaggcct
      301 ggactggaag atagagtcga ccttagacag aagagaagca ttgatagagg ctgattttgt
      361 aacgtctcag ttccgagttg gccaactacc tgcaagatac ttagatgaga ccattccctt
      421 gagttatggg atgctggggc aagagaccaa tggggctgga ggaatcttta aagcatttag
      481 gaccattgaa gcatataaag ggattatcaa ggatatgaag gacttgtgtc cgaatgcttg
      541 gttgattaat tttacgaatc ctgctggtat cgtaacggaa gccattgcta atgaactcca
      601 ctggaagaaa gttattggtc tgtgtaatat ccctattggt caaaagaaac ttgctgctca
      661 gattttaggc caatcagaag gaggcctaag tacgcgccat attgggttaa atcattttca
      721 ctttcatgaa gtttgggata agtatgggga aaatcgtact caagtagtct tagaaacatt
      781 gtatggggat ttgagagagg agacgccaga agcagtcaaa aatattacca atctagagtt
      841 tcctattcaa ttgcttcgga cgattggaat gttgccatgt gattaccatc gttattattt
      901 tttggaacaa gaaatgttag aggatgcttt ggaacagtat cgtcagggta atgtacgagc
      961 caaggttgtt caagaagtag agacagaatt gtttgagctt tacaaagacc cacaattgaa
     1021 agacaaacca aaacaactgg aactacgtgg gggtgcctac tatagtgatg ttgcctgtca
     1081 aatcatttgt gctatccata atgatagtgg agaagaattg accgtatcta ctgtaaatag
     1141 aggagttatt ccctatctac ctgaaaattg tgtggtagaa gtcacctcga ttatctctgc
     1201 gcaaggagca acaccactag cttgtccacc aactccgccg attgtaaccg gctatttaca
     1261 actgatgaag caaatggagc ttgtcactgt tcaggcagca atgacaggtg attattcact
     1321 ggctttacaa gcttttacac tcaacccgct tatttcaaac ggaccacaag ctgaagtgct
     1381 attacaagat atgttacttg cacatgaaaa ttatttgcca caattcgcac cagctataca
     1441 gcacataaaa gagagaagga acaatcaatg actaaagaca aggtatttta tcaatttcca
     1501 acaaattttt tgtggggttc ttctacatca ggacctcaaa gtgagggaat tgagccaggt
     1561 gatggaaaag gacagaacaa ctgggattat tggtatagta ttgagcctga acgatttcat
     1621 aacaaaattg gccctagtca aacatcaaca ttttacaaac attatgaaaa agatattgat
     1681 ctattagttg aaactgggca cactgtattt cggacttcca ttcaatggtc gcgtctcata
     1741 ccaacaggta caggagaaat caatcaagag gcggtgtcct tctatcgttc ggttttttct
     1801 ttggtaagag caaagggaat tcaactcttt gtcaatcttt atcactttga tcttccctat
     1861 tgtttacaag aaaaaggtgg ttgggaaagt aaggaagttg ttcgagccta tgaagcctat
     1921 gcacgtacat gttttgaatt gtttgatgac ttagtcgatc gttggattac ctttaatgag
     1981 ccgattgttc cagtagagtg tggttatcta ggaaattatc attatccatg taaggttgat
     2041 gcaaaggcgg cagttgcggt ggcttatcat acacagttgg caagttcttt agctgtgaag
     2101 gcatgccacg aagtaaatcc taataaaaaa atcgctattg tcttgaatct aactccagcc
     2161 tatcctcgta gtgagcaagc agaggatata aaagcagctc gaattgcaga attgtttcaa
     2221 acaaagagct ttctagatcc gtctgtgtta ggaaaatatc ccgatgaatt agtcgagctg
     2281 attgatgcat atgggctaat gccagaggtc agcgatgagg aattagccat tattaaggaa
     2341 aatcgtgtgg acttcctagg agtgaattat tatcaacccc ttcgggtaca agctccaacc
     2401 aatagttgga aagagggaga gcctctaact ccggaacatt tctttgcccc atatgatatg
     2461 ccaggtaaaa aaatgaatcc gcatagaggt tgggagattt atgaaaaagg gatatatgat
     2521 attgcgataa atattaagga aaattaccat aatatcgaat ggtttgtaac agaaaatggt
     2581 atgggagttg aaggagagag cgcatttaaa gacaatggac tcattcaaga tgattaccgg
     2641 attgaattta tcgaagatca cttaattgaa ttacatcgag ctatccaaga aggggcgaat
     2701 tgtaaaggat acatgttatg gacctttatt gattgctggt cttggttaaa tgcatacaag
     2761 aatcgctatg gattagttga actggatcta gacacgcaag aaagaattgt taagaaatca
     2821 ggcaagtggt tcaagaaatt gagcgatgct aatggttttc atcgctagga gggaatgatg
     2881 gtggaactgg gcttttcaat ttatcctgaa aactattcgc tagaagagag tcaagcttac
     2941 attaaacttc tgcaccaata cggagcaagg cgcctattta tgagtttgtt gcaacttgaa
     3001 aatggagacc gaacagtttt cgaattgtat caaagactaa ttgactatgc aaatcaatta
     3061 gggatgactg tcattgcaga tatcagtccc cattttatcg cagcgcaagg ttgggatggg
     3121 gcattgatgg agcgtgcagc tgaactgggg ttggctggga ttcgtcttga tgaagctctt
     3181 cctttagaag aaatcatcga gatgacacat aatccacaca acataaaagt tgaattgaat
     3241 ttgagtacag ataagatctt gctgtccaat cttttggcta gtgttgcaaa cagggataat
     3301 attattgcct gccataattt ttatcctcat gcttttacag gtttatccga agagcacttc
     3361 ttagagatgt catcttttta tcatcaatca ggtataagaa ctgcggcatt tgtcagtgca
     3421 caaacagctt ctgaaggacc gtggccatta tctgaaggcc taccaacttt agaagatcat
     3481 cggttctggc ctcttccctt gcaaattgct tggatgaagg caacgggttt tattgattct
     3541 attttgattt caaaccaatt tatcttggag gaggaactac aatccattca aacagtttta
     3601 aatgaagata caatcagcct catggttgat attgaggagg gtttgactcc cgttgaaaga
     3661 gagattgtag cgtttaatca tgtttataga ggtgatattt ctgctcatgt tctgcgttca
     3721 accatgcccc gtgtggtgta taaggacgca tctgtaccgg ctcggtcaga ccaagcaatt
     3781 cctgtgggac gaggtgatat cttaattgat aatgatttat atggtcgcta caagggtgaa
     3841 ttgcaaattg cactcaaaga atttaccatc agtccaaagg taaataaggt cggacgaatc
     3901 agcccagact atcttccctt gttacgctgt attaaaccgt ggcaagagtt taaactggtc
     3961 atagcttccg agcactttca ttaatgatgc ggaaaaagta ggtacctcta gaaatcgctt
     4021 ccatgttata atgtacatat aagtaattaa agactgttgg aggaaaggag ggagcatgtt
     4081 atctaaaaaa gaagtacgct tgttagaggc tttacttgca aaacaggaga cattcgtacc
     4141 aagcatcgaa ctagccaagg tattatcagt gagtgatcga acggtaagaa cgtatattca
     4201 tcaactgaca tcttctttag aaaatagcgg tgctcatatc ctttcaaaac aaggtagtgg
     4261 ttattgttta cagattgaac ggccagttga gtttgaaatg ttttggcagg agcaattgct
     4321 cctaaaaaag cggttaaccg atttaaccca actagaagaa gcgggggatc ggcaacggta
     4381 tattctcaat aagctcttgt ttgaggatcc gattcagtca ccagattgtt tacaaagaga
     4441 gctattcatt ggaaagacta cgcttcattc cattttggcg gatattcgaa aactatttat
     4501 cccctatcaa ctagaactcc ttgtgacacg taagggaatt gaactaaagg gggatgaacc
     4561 tgctatccgt cactttatta tggattactt ttttgatgag gggaatgttc aatctgttta
     4621 tggggttgtc gaaaccaatc tattgccgga tatacggttc acagacttaa tgttaatcgt
     4681 tttagacgag tgtcgggatg ctaaattacg tttatcggat tttgtcatgc agaatctagt
     4741 gctccatatc gctcttcttt tacaacggat gaaaatcggg tatggtctag accaattccc
     4801 aatatcggag catattcatt cgtctcaaga gtatcttgta gcacagcgga ttattgaacg
     4861 aatcgaagaa acgtttcaag tgacgatacc gtcagaggaa gcaaattaca ttgccttgca
     4921 ccttaaggta aaactaacgg ggaatccaaa tcaggatggt gaggtggaag agatatttag
     4981 acagaatgtg gagaatagtc ttctacattt gtcacattta acaggcctag agttgtcaaa
     5041 ggatcagcaa ctattcaaag gagttctggc ccatttgatg ccattacgta ttcgtttgga
     5101 gaataatatt caactgggaa atcctctaac tgctgaaatt aaagaccaat attttgaggc
     5161 tttcgaatta acaaaaattg catttgcagc gataccagaa ctaatggggt attcgatttc
     5221 ggatgatgag tgggcttacc ttaccttaca tgtgatggcg gcaattgaac gctatcaggg
     5281 atttaagaag accagagtat tggtagtctg tgctaccgga attggaagtg cattgatgtt
     5341 aaagaatcgc ttggagaaag aattttcatc cagcttagaa attcttgata cggttagtta
     5401 ttatgaagtt acagaggaac gactgaagga tgttgatttg attatttcat ccattagctt
     5461 gtcgaatcta atttttatga caccggttat caatgtcagc gtcttcctat cagagaagga
     5521 cattgaggct attcgatctc atttacagca aacgacccca gttattcctg accaagggga
     5581 aaatgtgcta atggaagtag gtcaggctaa gcagattgca gaagcagtat ttgctccaca
     5641 tagaataatc tatcttgagg aggattgtga tcgagaagta gccttgaagc aactagtaac
     5701 tactttgaac gaatccattg agcctgattt tgttaatcgc tttatagaac aagtgaattt
     5761 acgagaatct tacagtccgg ttgtctttgg ggatcggtta gccttccctc atccagcctt
     5821 gccgatgagt tattcagaac aaattgttgt agggatatat aagaatgctg tggattgggg
     5881 tcatggaaag caagttcaat tcgtcttcct attgtcaccg tcaaagggtc gaaatgcatt
     5941 tctgaagtat atctcacctt gtctggttga ttttgttcaa gatagagttc tccaagaacg
     6001 tttgttacat catccaaatt ataaagaatt aattgatatt tttattcccc ttattcaaag
     6061 aaaggggtag gaggagtttt ttatgaaaca cattatgtta gtttgtaacg caggcatgtc
     6121 tacaagtatg cttgtaacta agatgcaaaa agcagcagaa gaaactgggg ttgaagcaac
     6181 tatttgggct gttccagtat ctgaagccga taatgaagtt gctcaaaaag agattgatgt
     6241 attactctta ggtccacaag taaaattcct attgaacgac tataaggcaa aatttgagcc
     6301 aactattaag gtagatgcta tcaatatgat ggattacgga gtgatgaatg gagctaaggt
     6361 gctagaggct gccctaaaaa tgatggaggg gtagtatgaa ttctccagag tatcttgaag
     6421 cgattatggg gttaatcatg tacggtggtg aagcaaaggg cttcgctagc caggcgattc
     6481 gagcagccaa agaaggtcgt attgacgaag cggaacagct gctccaagac gcaatagctt
     6541 cattgaatat tgcccataaa tcgcaaacac aattattagc aaaagaagca agtggagagt
     6601 ctgttgaatt atctttgtta atggtacatg gacaagatca cttgatgaat gctttgacat
     6661 ttattgattt agcgaaagag ttcgtagatc tttataaaca gattggataa ttataggagg
     6721 actgggttat gaagatatca aaaataggtc aagttccaat tgagcgactt ttcgctacta
     6781 ttcgccaatc cttaaaagat gattttcagc aaaatacggg ttccatcttg caagatcaag
     6841 acattatcca gggacttact tatactaaaa catttggtga tagaggacaa catagtgtca
     6901 aggtaactgt agaagaattt acagctcctt atttctatgc agtttgtttc cattctaatc
     6961 gaggaaaaga gcatgttcgc taccagttag aggatttagg gaatgctcaa accaatataa
     7021 aatattctta tgagatggag gctggtgata tttttgtaaa aggcaatcaa tttctcatgg
     7081 aaaaaatctt ttcaaaaagc ataaaacgtc agacaaatgc ccagttagag gcgctgattc
     7141 gttgctcagt tgagccggta tcagtttaac atttaggaca tataaaacaa aaaatagaaa
     7201 aggagacaga atatgtcaaa tatttctgag aaattcatgg aaatatctgg gaaaattggt
     7261 tcgcaacgtc atttgattgc gattcgtgat tcctttattt ctatgatgcc cattacgatg
     7321 gcaggtacag tagccgttct attgaatgtc tttctgagag acatcccaaa taatatggga
     7381 tggactggtt ttgctgatgc catgcaacca gtgatagata tcaatggtta cgtttatttt
     7441 ggaactatcg ggataatggc acttgtattt gcctttacct ttggttacaa tttgtcactc
     7501 atgtataagg tgaatccttt agcaggtggt ttaatttcct ttgcatcatt catctctact
     7561 attcctcaaa gtgtaacgat ttccactgct ttagacggag ttgataagtc agttgtttct
     7621 gctttggaag gtttgggatt atccattgtg acgacagatg gagtttcagc cttggaaaca
     7681 agccagtggg gagctatcgc attgaaatat ggcggtgcaa caggtttgtt tacagcattg
     7741 tttattggat ttctcgctac atgggtctat gcaagtcttg taaaacggaa tgtcattatt
     7801 aaattgccag attctgttcc accggcagtt aataaggcct ttgctgctat tatcccaggt
     7861 actgctgcaa tttatgcgtc atccattgtg gcatatcttg catttactct aacaggacaa
     7921 tctttgggag atttagtatc aacttatgtt caactgccat tacttggtct gtcacaaggt
     7981 cttgggtcag tcattctttt aactttcctt gtacaacttt tttggttctt cggcctgcat
     8041 ggtcacaatg tcttggcgcc aattatggac ggagtctata tggttgcact aaatgaaaat
     8101 gcggctgctt atactgcaac tcatagtact gcgaatttac cgtggctttg gactcgtggt
     8161 tcctttgatg cttatgctca aatgggtggt tcaggcgtca ctctagcttt gattattgct
     8221 attttcattt tctcaaaacg agaagaatac aaaatggtag ctaaattatc tgctccaatg
     8281 ggtgtattca atattaacga accagttacc tttggtatac caatggtatt gaatccactg
     8341 tttgctattc catggctaat tgttccaccg atttgtgcaa ctatcgcata cttagcgaca
     8401 gcggcaggta taattccgcc agtctttgct cctgttccat ggattacccc tccaggactc
     8461 tacgcttatc tagcaacagg tggtaacatt atggctgcgt tggtttcact attcaacctc
     8521 tttgttgcct tcctgatttg gacaccattt gttattgcag ccaataaggt taaggctggt
     8581 gaggaataat gttttccaag aacaaatctc ctttcttatt ggcgctagga tttttcctta
     8641 tcgcgattag tccgctttat gggaataaat atctaaacat tgtatctggt ttggttttta
     8701 ttctaatcag tgtactcagg agtaggagaa aataattaca ctaacactaa atagaaaacg
     8761 acttccgtat tggaggtcgt ttttttggca aaaagtccgg acactgtgat tgttaagaga
     8821 ttgcaagtta tccgtaatat ttgttacatt tctttaagct ctctgcaagg attatccctt
     8881 aggatagata gggcagaaaa acacgaagga atcaaatatt atgagttatc aaatgataaa
     8941 aaaacaattc aaattgagac tgtttacagc cctcatagga aatcttttat tgttgggaat
     9001 ggtgaatgtc agtgcagatg acacagttat aagcgatagt ccgacagtat cagcgttaat
     9061 aaaaaaggtt gaagctgcaa aaatagcagc caaagctgct gcagaaaaaa ttgtggctga
     9121 taaagctact gccgctgcaa aggctgcttt ggctaccaat atggtgtcag aggttgctgt
     9181 ggaatatacg gctaatacct atccagcggg tcagtgtact tggggtgcca aagaaatggc
     9241 tccttgggtt ggcaactatt ggggtaatgg tggagattgg gctgctagtg cggcagagct
     9301 tggatatgaa gtgggaacta ctccaaaagt tggcgctatt gctgtatgga cagatggagg
     9361 ctacggtcat gttgcctatg taacagatgt ggcagctaac ggtcacattc aagttaagga
     9421 atcaaactat tgtggagtat actacccaag taatgtccga ggtttctttg atccaacaac
     9481 aactagcgaa ggaacagtca gctatattta cccaccagct ggagtataat taatatgaga
     9541 tataaagacc gagtaggtct cagattgaag aaaaagtcca ttttggacaa ttttcttcaa
     9601 tcttttttct ttatgttgaa aataaaaaag ctctcagaaa tactaaaata taaatgcttt
     9661 caagagttta atgacttgct atctttcgca aacttcttca attttttata ttgttaggag
     9721 tttgtctacg ttctgagacc aagtaggtct ttttttatga tccgatactt gattaaaatt
     9781 tttaatgtcc ctgatagaaa aaatatattg gctggaaatt gttattcatg atactattgt
     9841 aaaatctgaa aaagaaagtg tgttttaatt atgaagaaaa tagctatgtt tacaagttta
     9901 ttgtttgcgg gtatcgtgtt aacagcctgc aattcatctg caggtacatc gaatagtaca
     9961 gaccaaaccc tcttggcaca aattaaggaa gaaggcgtta ttcaaatcgg tactgaaggt
    10021 gcctatgctc cttactctta ccacgatgag agcggaaaat tggtcggtta tgatgtagaa
    10081 gtagccgaag ccgttgctga gaaattgggt gttaaggtgg aatttgttga aacaaaatgg
    10141 gattccatga ttgcaggttt ggatgcggcc cgttttgata ccatcgctaa ccaagtcggt
    10201 attacagatg agcgtaaggc aaaatacgat ttttcaacac catatacata catctatgga
    10261 gccttggtaa tacagaagga taatactgac attacaggct ttgcagattt atctggtaaa
    10321 aaatcagcca acagcttgac aagtaactgg gcagatttgg cgcgtgaaaa tggtgcagaa
    10381 gttgtcggtg tggatggatt ctctcaggca gttgaattgt taaatacagg gcgtgtggat
    10441 gctaccatca atgacaactt ggtatacttg gattatgtga aacaacatgc tgatgcaccg
    10501 attaagattg tgaccttgac ggatgacgta tcgacaacag ccttcccagt tgcaaaagga
    10561 aatgaagatc ttgtgaaaga gattgatgca gccttggcag aattggctag tgaaggaaaa
    10621 ttagctgaaa tttcaaacaa atactttggt gaagacgtat caaaagctaa gtaatcagga
    10681 gaaacaatga cagaaagagt atggcaacta ttgcttgatt cattttcaca gatacttgtt
    10741 ccaggattgt tggtgactat cccactgaca atcctgtctt ttacctttgg cttgttaatt
    10801 gctattggga cagccttggt gcaaattgct cagattccca ttctcaaaca agtggctcgt
    10861 ttctatattt gggttgttcg agggacacca ctcttggttc aactctatgt gatattcttt
    10921 gggctaccaa gtctaggaat tgtcttggat gcctttccgt cggcggtttt ggttttttct
    10981 gtcaatacag gtgcctacgc tgcggaaacc atacgggctt ctatcgagtc cgtgcctaag
    11041 gggcagttgg aggctggcta ttctgtcggg atgagttttg cccagaccat gcgtcggatt
    11101 attctcccac agggctttcg agtggccttc ccgcccttgt ccaatacttt gattggtctt
    11161 gttaaggaca cgtcattggc tgccaatatt accgtcttgg agatgtttat ggcgacccag
    11221 cagattgcag caaggaccta tgaaccgttt gccctctatt gtgaggtggc ccttgtttat
    11281 ctactctttt caactatttt gaccaagctt caagcctatg gtgagaagaa attagccgtt
    11341 tactaggagg ttcagatgat tcaagcaagt ggagtaaagc tgaccttcgg tggtcagcag
    11401 gtgttagaag gaattgatgt agaggtcaat ccaggtgagg tggtcgtcat tttaggtccg
    11461 tctggatcag gaaaaacgac tcttcttcgt tctttaaact ttttggaaaa agctgacgcg
    11521 ggtcacttag tcttcggtga ggacagctat gacttggctc agatgaccaa gaaggaaatt
    11581 gcaggcattc gcaagcgcac agccttcgtt tttcaaaact ataatctctt tgccaataaa
    11641 actgccttac aaaatgtgac ggaaggtctg attgtggctc ggagagtacc caaacaagag
    11701 gctgaaagaa tcggtaaagc agccctggac aaggtgggac tatctgataa atatggtttc
    11761 tatccaagcc agctgtccgg tggtcagcag caacgggtcg gcattgccag agccattgcg
    11821 accaatcccg aggtcatcta ttttgatgaa ccaacctcgg ccttggatcc tgagttaatc
    11881 ggtgaggtct tggctgtcat gatgcagttg gcaaaagaag gtatgaccat ggtggttgtg
    11941 acgcacgaga tgggctttgc gcgtgcggta gctgatcgag tcatatttat ggatggtggt
    12001 cggattatcg aagaagatcg accagaagac atttttgacc agccaaaaga agaacgtact
    12061 aagcaattct tagctcgcat tcaaaaggta gatgttgagt aagctaagca aattaattta
    12121 tcttattcat ttccataatc gtacagaaaa tggatagaat ttgacaaaga tacttcaatt
    12181 gttagacatt gattggtcta gcagttggag tattttttgt ttagagggaa tggatattct
    12241 tagat
//
LOCUS       DATUCG010000072        12190 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.073, whole genome shotgun sequence.
ACCESSION   DATUCG010000072 DATUCG010000000
VERSION     DATUCG010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12190
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.073"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            <1..942
                     /locus_tag="VCA20_001527"
     CDS             <1..942
                     /locus_tag="VCA20_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HEP1835333.1"
                     /translation="NEIFAPEYQRRELLWGVKRKARFIESLLLDYPIPLMFLADTDDG
                     TMEIVDGLQRISTLSEFFSKDENLKLDGLKKLDKLNGCTRSDLPEGEIRRLRARSLRV
                     IVLKHNTPSEVRKELFDRLNTSSLSANSSEVRSGRELDNELMKLIKELAEDELFRKVT
                     NLSENRINRKEDIELVSRFFAYSNALNYYSGHVQNFVDDFVTSSGQEWSDDKKEIYKQ
                     EFYNTMNFVYKNFKRGFQKEDRNQTPRVRFEALAVGINLALRENPELETTDQQVDKLL
                     QSVEFEEWTTSDAANNRSKVFSRINGVKDYFLTGNLS"
     gene            945..1175
                     /locus_tag="VCA20_001528"
     CDS             945..1175
                     /locus_tag="VCA20_001528"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835334.1"
                     /translation="MNRIEYEKLKQEIDRFIDFLIHLEHSIIGIDSNDCQSFKVDVLD
                     KDEAESVFKSMKSNAMIMLYNLVEACVRIGHL"
     gene            1681..2487
                     /locus_tag="VCA20_001529"
     CDS             1681..2487
                     /locus_tag="VCA20_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEP1835335.1"
                     /translation="MQPTYNIDNPHLSYEAKQELWETGFGLQKVDGLTPSVYMEELAE
                     RQARGEYTYEQVYEEITKYHQSTDASTQEADIVSLRIVEMLSQTGFSLRPTTLLHIHK
                     ELFQDIFDSSIPIGKYRTVNITKNEPVLKGDTVIYSDFPLIVATLDYDFRQERDFSYA
                     GLDKKAIIAHIQSFISGIWQIHPFREGNARTITVFLIKYLRSLGFDIDNEPFQKHAKY
                     FRDALVLDNAKLVNKRSDFLTAFFENLLLNGQNDLSSEMMYEELGLDGYQ"
     gene            2583..5296
                     /locus_tag="VCA20_001530"
                     /pseudo
     CDS             2583..5296
                     /locus_tag="VCA20_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
     gene            5412..6140
                     /locus_tag="VCA20_001531"
     CDS             5412..6140
                     /locus_tag="VCA20_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835336.1"
                     /translation="MEREIFTPNQSQAIVDAILKGYKSYLDERIEKRKSMHVSAGYAW
                     TKANHIDDALAVADLTFIDTFELKHAGQSWEFLEFNSENQLLGRVCFVLKGLTRLQQV
                     FPTAVKSGKGYLFDYAQINTSYLENLSRHTHTDESTLFPVQMELFDDNLFELLNRSEQ
                     SINNFFFITYQADELHHLTNIQVIMPDSRTGKLIEIQNLSTFISTSNVDFNDEKYQEL
                     QGLSEIDDVADFEIIPRLASEKEA"
     gene            6153..7307
                     /locus_tag="VCA20_001532"
     CDS             6153..7307
                     /locus_tag="VCA20_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEP1835337.1"
                     /translation="MFNGSRLEELRLLNGLTRADLARELEVTEQAIWQFETGVTLPKM
                     KNKIAMARFFQVEVDYFDAVDKNGSFSLSRIAFQNADLEAKKNIHIQMVYLEKMNQMI
                     DYLENFVSIPNQIIYQLVDSIEQKLQQHESLEFIADHAREVLAVSVDNANLLYSLEKS
                     GIYISERLINGNADAYSAWSRTGRPFIVLGLGKSAVRRNFDLAHELGHLLLHNQVDFE
                     ELSKEELEEKEKEANQFASYFLLPRKQFLMDFVNIVGKRVSNPDQYILLKKRFGVSIQ
                     ALEYRAYKLGLLTPQQNSYFYRQISKKGYKAVEPLDLEIPLKKPSKIRSILDVILSNN
                     LLTLSDIERNQRVSKNFISQLFSFDVKFFDKYLLQLDTFDNVIPLFSRKQ"
     gene            7322..8380
                     /locus_tag="VCA20_001533"
     CDS             7322..8380
                     /locus_tag="VCA20_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEP1835338.1"
                     /translation="MNKINSYKIHLPSLLPFGFLFANNCYTYREVFMEGQFEAVVEVD
                     EAGQLSSYVWDCEMEEIYTAHLVTAPAGAFVGQVREAYQSILDRVEEACCIALPFSKD
                     QSNRLAQLIKEQWGDLPDYPFAKLPTYGAFRHPNNNKWYALVSQIPRDKLDGSGSKEE
                     VEIVNLKVDGREIAELLSQSGIFPAYHMSKKTWVSVLLDDTVEDQTVFALLEKSRYLV
                     GPKSYKAAQGPDYWVIPANPKVYDIDTEFAENKIVYWPQKSTIQAGDIVAIYVTAPVQ
                     AIRYVCRVLGANLENRGESDIPTEKKLMQVELLAQFSDDVLPRARMIDLGVKAVRGPR
                     RLTKELIDVLTSEVINIY"
     gene            8464..9456
                     /locus_tag="VCA20_001534"
     CDS             8464..9456
                     /locus_tag="VCA20_001534"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HEP1835339.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLKDKLEIVEVQGPILSRVGDGM
                     QDNLSGVENAVTVNVKLIPDATYEVVHSLAKWKRHTLARFGFHEGEGLFVHMKALRPD
                     EDSLDPIHSVYVDQWDWEKVIPDGRRNLAYLKETVEQIYKAIRLTELAVEARYDIESV
                     LPKKITFIHTEDLVKNFPDLTPKERENVVAKEYGAVFLIGIGGELADGKPHDGRAPDY
                     DDWTSPSEAGYKGLNGDILVWNEALGSAFELSSMGIRVDEEALKCQVAITGDEDRLEF
                     DWHRALLNGLFPLSIGGGIGQSRLAMFLLRKKHIGEVQSSVWPQEVRDSFENIL"
     gene            9590..11437
                     /gene="typA"
                     /locus_tag="VCA20_001535"
     CDS             9590..11437
                     /gene="typA"
                     /locus_tag="VCA20_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEP1835340.1"
                     /translation="MTKLREDIRNVAIIAHVDHGKTTLVDELLKQSQTLDERTQLDER
                     AMDSNDIEKERGITILAKNTAVAYNGTRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQNLTPIVVVNKIDKPSARPEEVVDEVLELFIELGADDDQ
                     LEFPVVYASAINGTSSLSDNPADQEETMAPIFDTIIEHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERKEIQEAKAG
                     DLIAVSGMEDIFVGETITPTDAVEPLPVLRIDEPTLQMTFLVNNSPFAGREGKWVTSR
                     KIEERLLAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IIKEIDGVKMEPFERVQIDTPEEYQGSVIQSLSERKGDMLDMVATGNGRTRLVFLVPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPVVQGEIGGRHRGALVSIDQGKATTYSIMR
                     IEERGTIFVNPGTEVYEGMIIGENSRDNDLTVNITTAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAERDKAAKKKKLATEE"
     gene            11455..11745
                     /locus_tag="VCA20_001536"
     CDS             11455..11745
                     /locus_tag="VCA20_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HEP1835341.1"
                     /translation="MFYLILAIMLVLFYIFIAPRNIKGTMNLIASVFLLVALAIALVL
                     GFLRIMQFPKEIWVGLLMILIGFWAMRDIYYLDKEPKDRQRRTKRPGPYRYR"
     gene            complement(11809..>12190)
                     /locus_tag="VCA20_001537"
     CDS             complement(11809..>12190)
                     /locus_tag="VCA20_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEP1835342.1"
                     /translation="VTPREVVQKTLDFSDELKFYYELYQILLFHFQDMNSKYFFELLE
                     DNLDFVNPAFKTVFKTFLKYKTYITNAMELPYSNAKLEATNKLIKDIKRQACGFRNFT
                     NFKTKIYIASNIKNERTNFVLSRC"
BASE COUNT         3754 a         2143 c         2672 g         3621 t
ORIGIN      
        1 aatgaaattt ttgcaccgga atatcaaaga agagaattac tttggggagt gaagagaaag
       61 gctcgattta ttgaatcatt gttgctagat tatccaattc ctttgatgtt tttagcagat
      121 accgatgacg gaactatgga aatagttgat ggtttacaaa ggatttcaac cctatctgaa
      181 ttttttagca aggatgagaa tttaaagctt gatggcttga aaaaacttga taaattgaat
      241 ggttgtacta gaagcgattt accagagggg gaaattcgaa ggctgagagc tagatctctc
      301 cgtgtcatcg ttttgaaaca caatactcca tcagaagtcc gaaaagaatt atttgataga
      361 ttaaacacaa gctctcttag tgctaattca agtgaggtta ggtcaggtag ggagctagat
      421 aatgaattaa tgaaattaat aaaggaattg gctgaagacg aactattccg taaggttact
      481 aatctttcag aaaatcgtat taatagaaaa gaggacattg aattagtatc gcgtttcttt
      541 gcatatagta acgctttaaa ctactacagt ggtcatgtcc aaaattttgt agatgacttt
      601 gtaacctcat cgggacagga atggtctgat gataaaaaag aaatttataa acaagaattt
      661 tataatacaa tgaattttgt ttataaaaat ttcaaaagag gctttcagaa ggaggacaga
      721 aatcaaacgc ctcgtgtcag gtttgaagct ttggccgttg gaataaatct tgcactacgt
      781 gaaaatcctg aattagagac tactgaccaa caagtagata aactgttgca gagtgtagag
      841 ttcgaggagt ggactacatc agatgcagcc aacaatagga gtaaggtttt tagcagaata
      901 aatggcgtta aagactattt tttgactggt aacttgagtt agtaatgaat agaattgaat
      961 atgaaaaatt aaaacaagag attgatcgtt ttattgattt tctcatacac ttagaacatt
     1021 ccataattgg tattgatagt aatgattgtc aaagctttaa agttgatgtt ttagataaag
     1081 atgaagcaga atctgttttt aagtcaatga aatcaaatgc tatgattatg ctttacaatt
     1141 tggttgaagc gtgtgttcgt atagggcacc tctaaaaact tcaagtagtt gttagaggtg
     1201 cccatcaagg atatgagaaa tagtctggca cacggtaata tctcgtttga agaaaatggg
     1261 aaaaagcttg ctgttgatga tatatctcaa tataaaactg aaacgtatct atgtctggag
     1321 tattttatga aagtcgttca agaggctgaa ttttaattat tgttatttta attttacagt
     1381 ttcatttaca atatgatact ttaataacgc caaaccagaa gtcgttgaaa aagaaaaaag
     1441 ccgactacca agctaagtat ggtgctacag tagcgcggat tgaagagatg aagaaactgg
     1501 tctagtggcc taacatcact tgatacttcg tcagctcacc ttgccgtact caagtactgc
     1561 ctgcggtgtc gtttcctagt ctcaagctga tgttaaccgc actagattaa acaaatgccc
     1621 aacctaaaaa ggttgggttt tatggtataa taaggctaga aattcaaata ggaggagtct
     1681 atgcaaccaa cttacaatat tgataatccc catctttcct acgaagccaa gcaagaactc
     1741 tgggaaacgg gttttggtct tcaaaaagtg gatgggttga ctccgtctgt ttatatggag
     1801 gagttggcgg aaaggcaagc tcgtggagag tacacctatg agcaggtgta cgaggagatt
     1861 acgaaatacc accagtcaac tgacgccagt acgcaagagg cagatattgt ctctctccga
     1921 attgttgaaa tgctctccca aactggtttt agcctccgac caaccaccct cttacatatc
     1981 cacaaagagc tttttcagga tatttttgat agcagtattc caattggtaa gtatcgaacg
     2041 gttaacatta caaaaaacga gccagtttta aagggggaca ctgtgattta ttcagatttt
     2101 cccttgattg tcgctacttt ggattatgat ttccggcagg agagagattt ttcttatgct
     2161 ggattagata aaaaagccat tattgcccat atccaatcat ttatttcagg tatttggcaa
     2221 atccatccat tccgtgaagg gaatgctcga acaatcaccg tatttctcat caaatacctt
     2281 cgctcacttg gtttcgacat tgacaacgag ccttttcaaa aacatgccaa atactttcga
     2341 gatgctcttg tacttgacaa tgctaagtta gtcaataaac gctcagactt tttgaccgcc
     2401 ttctttgaaa atctcctctt gaacggtcag aatgatttat caagtgagat gatgtacgag
     2461 gagttggggt tagatggata tcaataaaga caggatagtt ttcaatccgg aaatagacca
     2521 gtgaatactt gggcttttga cttttcgttg atagtctcaa taggcaattt ggaggaactc
     2581 acatgtctta ttattatgaa aaattaacgg gtaaggttgc aaagcatctt gctcgttttc
     2641 ctattatgct accgataaga ttctgaattt aatgcaattt caagataaca atcagcaatt
     2701 tctgataagt gataagcatt tgtatgatta ctttgaagaa caaaaacatc aactaagtac
     2761 cgatgaaaaa ctctccattc tgttcagctt atttaaggga cgtgtagatg tttatgcgaa
     2821 atcttatatt gatgaaaatg gaaaaatcaa ctattttcct tcttacaact atggttggaa
     2881 aaaacttcct gttgaaaaga gaacttgtca accattgacc aaacaggtat tattggctca
     2941 cctacgtggc gaaatcagta ttggcatttt tccaatgagt ttatcagaca cgtgttcctt
     3001 tttggcgatt gattttgata aaaataattg gagagaagag gtctccattc taagagatac
     3061 agcagaacaa catggttttg aggggcatat cgaaatctct cgttcgggca atggtgctca
     3121 tctctggttt ttcttcgagg aagaaattgc ttgtcagcaa gcaagaaatg ttgggaaacg
     3181 attgctggaa ttggctatgc aggtgtcaaa agatattcgc ttttcttcat ttgaccgtat
     3241 gtttccaaat caagatattc tgcccaaggg aggatttggc aatcttatcg cactgccctt
     3301 acaaggggaa gctttcaaga agggaagaac cgtttttgtt gataaacatt ttcagccctt
     3361 ttctgaacaa tggacctatc ttcaacaggt tcaaaagatt ggacagaaga aaattctaga
     3421 tttcttagga caggagtttt cggatactgt agatgataca gtattagatt gtagcttatc
     3481 aaacgttatt caagttgaga agagggctat ttctgcaaaa acaaattact tattgagaaa
     3541 gctggcatct tttcctaacc ctgaatttta tctaaaacaa gcgaccagac aaccaaccta
     3601 tcagactcca gaacgcatct atttatttga agaaacaaac gaagccttgc atcttccaag
     3661 aggaatactt gctaagctac aggaaatctt tgaaacggta actattaggg acaatcgcaa
     3721 cagtttgtct ccgattcaga tttctttcaa agggaggttg cgttttgagc aggaacttgc
     3781 tttggcagat ctattagcta gtgagaatgg cttgctatgt gctgaaacag gtttcgggaa
     3841 gacagtttta ggggcagcct tgattgccca gaggaaatgt agaacgatta tcttagttca
     3901 caatcgtcaa ctgttggagc agtggttgga aaggttgggt gaatttttag agattgaaga
     3961 ggaagaggca gttcgctata ctccttcagg gcgtgtaaaa gtaataggcc atattgggca
     4021 atattgggct tctaaaaaat ggaggagtaa attggtggat gtggttatga ttcaatccct
     4081 cttccaattg gacgctattt ccgacttttt gtcggactac gatatgatga ttgtggatga
     4141 atgccatcat gtgacagcct tgcagtttga aaaagtagtt gctcaatttg ctagtcaata
     4201 tctatatggc ctaacagcaa cgccagagcg taaaaatgga catcaaccga ttgtctttca
     4261 gcgtattggt ccaattttac atactgctca atcaggtcaa tacgatttta aaaagaggtt
     4321 actactccat ctaacatctt ttgggaaatt ggacttggaa cagtcgaata gtacgaactt
     4381 tgcatccctt aatgattggc ttgccaaaga tttacatcgt aatactttga tcgtgcaaga
     4441 tattttcaaa ctatatcaag aaaaacgaaa catccttgtc ttagtcaatc gacgggaaca
     4501 tatagaatta ttggaaaagc tacttattga aaaagaaatg cccaatattt tttgtctgag
     4561 tggagcgagt aaaagaagag atacgaaaga actcctgaaa agaatcagtg agctagatga
     4621 gaatagtcca tttgttctca tttcaacagg aaaatttatt ggagagggct tcgatatgcc
     4681 aaagttggat acattaattt tggcagctcc tttgtcttgg aaaaataatc taatacagta
     4741 tgcagggcga cttcaccgtc cctatcaagg caaaactgag gtgagaatag ttgattatct
     4801 agatatccac gttccttatc ttgaaaaaat gtaccagaaa cgtcagatag cctatcgaaa
     4861 aatggcctac caagttggag aaaaggagca aacccaagta ttattcagtg gtagggacta
     4921 cgaggagaaa tttagagagg atttgcggaa cacacgttca acagtccatt tacaattgca
     4981 ttcgttttcg tctagtaaga ttcaggaatt attgggactg cttctaggaa aacaagtcgt
     5041 tattcatatt tccaaaagcc ataagttaag tgagtggcta atggggttaa acagtgacaa
     5101 tgtaaaggtg aagcttgaac cagaaaggat tggtacgacc gcagttatct tagatagtaa
     5161 tctcgtttgg tatggcaatt tttccccctt cacatatcag tcggatgacc aagctagttt
     5221 gttgaggttg gaaagtcaag ctatcgctga ggagttgctt gaaaaatttg aagacttaaa
     5281 tttaaacatt agataatgaa caaaatcccg aaaattttgt tcggaatttt ctttagaaaa
     5341 tatatcattt ttgtagtgaa atttaatgtt ttttataaaa atatgataga atggtacaaa
     5401 tggaggtgtt tatggaacga gaaattttta ccccgaatca aagtcaagct attgtagatg
     5461 ctatattaaa aggatataag tcttatcttg atgagcgaat tgaaaagaga aaatcaatgc
     5521 atgtttctgc gggttatgct tggactaaag cgaaccatat cgatgatgca cttgcggttg
     5581 cggacttaac ctttattgat acatttgaat taaaacacgc tggtcaatct tgggagtttt
     5641 tagaatttaa ctcagaaaat caactgttag ggcgtgtatg ttttgttttg aaggggctga
     5701 cgcgcctgca acaggttttt ccgacagcag taaaaagtgg aaagggctat ttatttgact
     5761 atgctcaaat taatacaagt tacctagaaa atctttcaag gcatacgcat accgatgaaa
     5821 gtacgctttt ccctgttcag atggaattgt ttgatgacaa tttatttgaa cttttgaatc
     5881 ggtctgaaca atctatcaat aactttttct ttatcacata tcaggctgat gaattacatc
     5941 acttgacgaa tattcaagtt atcatgcctg attcaagaac tgggaaattg attgaaattc
     6001 aaaatttatc aacatttatt tctacaagta atgtcgattt taacgatgaa aaatatcaag
     6061 agttacaagg cttatcagag attgatgatg tagctgattt tgagattata ccacgtcttg
     6121 cttcagaaaa agaagcttag aaaggcaaaa aaatgttcaa tggttcaaga ctggaagaat
     6181 tacgacttct gaatggcctt acacgtgcag atttggcaag agagcttgaa gtgaccgaac
     6241 aggctatatg gcaatttgaa actggagtta cacttccaaa aatgaaaaat aagatagcaa
     6301 tggctcgttt ttttcaggtt gaagttgact attttgatgc tgttgataag aatggttcat
     6361 tctcactaag tcgcattgcc tttcagaatg cagaccttga ggcaaaaaaa aacatccata
     6421 tccaaatggt atatttggaa aaaatgaatc aaatgataga ttatttggag aactttgtat
     6481 ccattcctaa tcaaataatt taccagttag ttgatagtat tgagcaaaaa cttcagcaac
     6541 atgaatcact tgagtttatt gctgatcatg ctagagaggt attggcagta tcagttgata
     6601 atgccaatct cctctatagt ttagaaaaat ctgggatata catttcagag agattaatca
     6661 atggaaatgc ggatgcctat agtgcttggt cacgtactgg tcgacctttt atcgttttgg
     6721 gactgggaaa atcagcggta cgacgtaatt ttgatttagc acatgaacta gggcatctac
     6781 tcttgcacaa ccaagtagat tttgaggaac tgtcaaaaga agaattagag gaaaaagaga
     6841 aagaagccaa tcaattcgcc tcctatttcc tgttacctag gaaacaattt ttaatggatt
     6901 ttgtgaatat tgtaggtaag cgtgtcagca atcctgatca atatatcttg ttaaaaaaac
     6961 gttttggtgt ctcaattcaa gcgcttgaat atagagccta caagcttggc ttactgactc
     7021 cacagcaaaa ttcttatttc tatcggcaga tttcaaaaaa aggatataaa gcagttgagc
     7081 cactagattt ggaaattccc ttaaagaaac caagtaaaat cagaagtatc ttagatgtca
     7141 tcctttcaaa caatctttta actctgtcag atattgaacg aaatcagagg gtgtcaaaga
     7201 attttatcag tcaattattt tcatttgatg tgaaattttt tgataagtat ttgcttcaat
     7261 tagatacgtt tgataatgta attccattat tttctagaaa acaatgatgg ggagtagagt
     7321 aatgaacaaa atcaattctt acaaaatcca cctcccctct ctcctccctt tcggtttcct
     7381 tttcgccaac aattgttaca cctaccgaga ggtgtttatg gaggggcagt ttgaggcggt
     7441 ggtggaggtt gatgaggctg gtcagctgtc gtcctatgtt tgggattgtg agatggagga
     7501 gatctatacc gcccatttgg tgactgcgcc agctggggct tttgtggggc aggtacgtga
     7561 ggcttatcag tccattttgg atcgggtaga ggaagcctgc tgtatcgctc tgccattttc
     7621 caaagaccaa agcaaccgtc tagcccagct catcaaggag cagtggggtg accttcctga
     7681 ctatcccttt gccaagttgc cgacttatgg ggcttttcgc catcccaaca ataacaagtg
     7741 gtacgccctg gtcagtcaga ttccgaggga caagctggat gggagcgggt ctaaagaaga
     7801 ggtggagatt gtcaatctca aggtcgatgg tcgggaaata gcagagctgc taagtcagtc
     7861 gggcattttc ccagcctatc acatgtctaa gaagacctgg gtgtcggtct tgctggatga
     7921 tacggtggag gaccagacgg tgtttgccct ccttgagaaa agtcgctatc tggtgggacc
     7981 aaaatcctac aaggcggcgc aagggcctga ttactgggtc attcctgcca atcccaaggt
     8041 ctatgatatt gatacagagt ttgcggaaaa taagatagta tattggccac aaaaatcaac
     8101 tattcaagct ggagatattg tagccatcta tgtgacggcg cctgtacagg ctatccgcta
     8161 tgtctgccgt gtcttggggg cgaacttaga aaatcgtgga gaatcagaca ttcctacaga
     8221 gaagaaactc atgcaggtgg aattgcttgc tcagttttcc gatgatgttt tgccgagagc
     8281 gcgcatgatt gacttaggtg tcaaggcggt acgtggtcct agacgcctca caaaggagtt
     8341 gatagacgtt ctgacatcag aagtgataaa tatttattaa attatcagaa tatttaataa
     8401 aaatctttac atctgcaaaa aaagcggtat aataagacta ttaaaattta aggaggagta
     8461 ggtatgaaga aaagcttcat tcatcagcaa gaggagattt cttttgtcaa aaatacattt
     8521 acccagtatt taaaagataa gctagaaatc gtagaagtgc aaggaccgat cttgagtcgt
     8581 gtcggggatg gtatgcagga taatttgtcc ggtgtggaga atgcagttac cgttaatgtg
     8641 aagctgatac cagatgctac ttatgaagtg gttcattcgc ttgcaaaatg gaagcgtcat
     8701 accttggctc gctttggatt tcacgaagga gaaggtcttt ttgtacatat gaaagccttg
     8761 cgtccagatg aggattcctt ggatccgatt cactcggtgt atgtggatca gtgggactgg
     8821 gaaaaggtca tccctgatgg tcgtcggaat ttagcttact tgaaagaaac agtagagcag
     8881 atttacaagg ctatccgtct gaccgaactg gccgttgaag ctcgttatga cattgagtct
     8941 gttttaccaa agaaaatcac ctttatccac acggaggatt tggtcaagaa cttcccagac
     9001 ttgacaccaa aagagcgtga gaacgtggtt gccaaagaat acggtgcggt tttcctaatc
     9061 ggtattggtg gagaactggc agatggtaaa ccacatgatg gacgtgcacc agactatgat
     9121 gactggacaa gcccatcaga agcaggctac aaaggtctca atggggacat cttggtttgg
     9181 aacgaagcac ttggatcagc atttgagttg tcttcaatgg ggattcgtgt agatgaagaa
     9241 gcgcttaagt gccaggttgc tattacaggt gacgaggacc gtttggaatt tgattggcat
     9301 cgggcccttt tgaatggtct cttcccgctg tctatcggtg gcggtatcgg tcaatcccgt
     9361 ttggccatgt tcttgttgcg taaaaaacat atcggtgaag ttcaatctag cgtatggcca
     9421 caggaagtgc gagatagttt tgaaaatatt ttgtaagagg ttgattttca atctcttttc
     9481 tttttcttcc aatcaactgc ctgaaaacat ttacaacaag attatctgtg atttttgcgc
     9541 caatctatga tatacttttt tagttaaatt aaaatatata gaggatatta tgacaaaact
     9601 tagagaagac atccgtaacg ttgccattat tgcccacgtt gaccacggaa aaacaaccct
     9661 cgttgatgaa ttattgaaac aatcccaaac acttgatgaa cgtacgcaat tggatgagcg
     9721 tgccatggac tcaaacgaca tcgaaaaaga gcgtggtatc actatccttg ctaaaaacac
     9781 tgctgtagct tacaacggta ctcgtatcaa tatcatggac acaccagggc acgcggactt
     9841 cggtggtgag gttgagcgta tcatgaaaat ggttgacggt gttgtccttg tcgtagatgc
     9901 ttacgaaggt accatgccac agactcgttt cgtattgaaa aaagcccttg aacaaaactt
     9961 gacaccaatc gtcgttgtca acaaaatcga caagccatct gctcgtccag aagaagttgt
    10021 ggatgaagtg cttgaactct tcatcgaatt gggtgcagat gatgaccagt tggaattccc
    10081 tgttgtttac gcgtcagcta tcaacggaac ttcatctttg tctgataatc cagcggacca
    10141 agaagaaaca atggcaccaa tctttgatac aatcatcgag catatccctg caccagtgga
    10201 taactcagat gagcctttgc agttccaagt atcccttttg gactacaacg acttcgtagg
    10261 tcgtatcggt atcggtcgtg tattccgtgg tactgttaaa gttggtgacc aggttaccct
    10321 ttctaaacta gatggcacaa cgaaaaactt ccgcgttaca aaacttttcg gtttcttcgg
    10381 tcttgagcgc aaagaaatcc aagaagcaaa agctggtgac ttgattgccg tttcaggtat
    10441 ggaagacatc ttcgttggtg aaactataac accaactgat gcggttgagc cacttccagt
    10501 tcttcgtatc gatgagccaa ctcttcaaat gactttcttg gtcaacaact caccgtttgc
    10561 aggtcgtgaa ggtaaatggg taacatcacg taaaatcgaa gagcgccttt tggccgagtt
    10621 gcaaacagac gtttctctcc gtgttgaccc tactgactct ccagataaat ggactgtttc
    10681 aggtcgtggt gaattgcact tgtctatctt gattgaaacc atgcgtcgtg agggctatga
    10741 gcttcaagta tcacgtccag aagttatcat caaggaaatc gacggcgtga aaatggagcc
    10801 attcgagcgc gtgcaaatcg atactccaga agaataccaa ggttctgtta tccaatccct
    10861 atctgagcgt aagggtgata tgttggatat ggttgcgaca ggtaatggtc gaactcgtct
    10921 tgttttcctt gtgcctgcac gtggtttgat tggttattca actgaattcc tttcaatgac
    10981 tcgtggttac ggtatcatga accatacttt cgaccaatac ttgccagttg ttcaaggtga
    11041 gatcggtggt cgtcaccgtg gtgcccttgt ttctatcgat caaggtaagg caactactta
    11101 ctcaatcatg cgtatcgaag agcgtggtac catctttgta aacccaggta ctgaagtgta
    11161 cgaaggaatg attatcggtg aaaactcgcg tgataatgac ttgacagtta acatcacaac
    11221 tgctaaacaa atgaccaacg ttcgttcagc taccaaagac caaacagcgg ttattaaaac
    11281 accacgtatc ttgactcttg aagaatcact agaattcttg aacgatgatg aatacatgga
    11341 agtaacgcca gaatctatcc gtcttcgtaa gcaaatcttg aacaaggcag agcgtgataa
    11401 ggctgcgaag aagaaaaaat tagcgactga ggaataattt ctaggagaag aagcatgttt
    11461 tatttaatcc tcgccatcat gttagtactt ttttatattt ttattgcacc tcgcaatatt
    11521 aaggggacta tgaatttgat tgcgtcggta tttttactag tagcattagc gattgctttg
    11581 gtacttggat ttttaagaat catgcaattt ccaaaggaaa tttgggttgg tctactaatg
    11641 attttgattg gtttttgggc gatgcgggat atttattatc tcgataaaga accgaaagac
    11701 aggcagagga gaacaaagcg accgggtcct tatagatata gatgaaatta accgacgctt
    11761 tgcgtcggtt tttcggctct ttgtcaactg tagtgggtag acgaaaagct aacacctaga
    11821 gaggacgaaa ttcgttctct catttttgat gtttgaagca atatatatct tggttttaaa
    11881 gttcgtaaag ttcctaaagc cgcacgcttg cctcttgatg tctttgatga gtttattggt
    11941 cgcttccagc ttagcgttgg aatatggaag ctccatggcg ttcgtgatgt acgttttata
    12001 ctttagaaac gttttaaaaa cagttttaaa ggcaggattg acaaaatcca gattgtcttc
    12061 tagaagctcg aagaaatatt tcgagttcat atcttggaaa tggaagagaa ggatctggta
    12121 gagttcgtaa tagaatttca attcatctga gaaatccaat gtcttctgga caacctctct
    12181 cggtgttact
//
LOCUS       DATUCG010000073        12182 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.074, whole genome shotgun sequence.
ACCESSION   DATUCG010000073 DATUCG010000000
VERSION     DATUCG010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12182
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.074"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            <1..161
                     /locus_tag="VCA20_001538"
     CDS             <1..161
                     /locus_tag="VCA20_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEP1835343.1"
                     /translation="AEQGHRLLPLPPYSPEYNPIEKTWAHIKKHLRRVLPNCDTFLEV
                     LSSCSCFS"
     gene            164..811
                     /locus_tag="VCA20_001539"
                     /pseudo
     CDS             164..811
                     /locus_tag="VCA20_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636572.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
     gene            complement(855..1637)
                     /locus_tag="VCA20_001540"
     CDS             complement(855..1637)
                     /locus_tag="VCA20_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP1835344.1"
                     /translation="MFKKLLKYEFQSVGKWYLGIYIAAVVLSAVLGFWLQALALRTQA
                     GNTEPGGGEMVLLGTSFMTFGILIAALFLSTFVLVINRFRKNVYGRQGYLTMTLPVSS
                     HQIILSKLLASIVWYILAGITCLLSIGIILAVILAASEEAIMPEIQTFVQAIDWTVLF
                     SQIVYYLIEITTGILLIYFSISVGQLFKDHRFLFAILTYIGILIVTGVFSTFFVLSNL
                     GTAYSTPFSLYPSPVLALVNIILAFAYYFGTHFIMTKKLNLQ"
     gene            complement(1654..2361)
                     /locus_tag="VCA20_001541"
     CDS             complement(1654..2361)
                     /locus_tag="VCA20_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1835345.1"
                     /translation="MSNAYTLVELQQVSKSYGGAVALNNVNLKLAAGKIIGLLGPNGS
                     GKTTMIKLINGLLQPEYGQVLINGRTPSPETKAIVSYLPDTTYLDEHMKVLEAITFFE
                     DFYADFDKERALHLLQDLGIDLNSRMKHLSKGNKEKVQLILVMSRQAQLYVLDEPIGG
                     VDPAARDYILKTIVNNYSPTASVIISTHLISDVEQILDEVIFLHYGSVIRHGNVDDLR
                     IESGESIDELFRQDFKA"
     gene            complement(2354..2728)
                     /locus_tag="VCA20_001542"
     CDS             complement(2354..2728)
                     /locus_tag="VCA20_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEP1835346.1"
                     /translation="MAWKFDNNIPIYIQISNTIKLQIVTNQLKSGDKLPTVRDLADIA
                     GVNPNTVQRALSDLETEGFVYSVRTTGRFVTDNQELITQTRLHLAQKELENFVNNMLD
                     LGFKHDDLTSQLDDYLKGVTYE"
     gene            2883..5993
                     /locus_tag="VCA20_001543"
     CDS             2883..5993
                     /locus_tag="VCA20_001543"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEP1835347.1"
                     /translation="MLAQLDTKTVYTFMDSMVTIENYVGKAKELGYHALGIMDKNSLY
                     AAYSFMESCNKMGLQPIIGCELDWKLRGGEQDVTTQLIALTTRGYRNLLKISTAKMTG
                     QDDFETIRQYLVDIAVVVPYFSGIEHLDLGVNYYIGVTVDTPVQDFKQAPLPIHTVRF
                     FEEEQLESLQVLHAIRDNIPLNQVSDVDSRQKLLSADSLTNLFSNRFPQAVDNLRDLV
                     AGISYQLNTDLKLPRFNRDRPAVEELYERSYQGLEEKGLTNSLYKERLEKELSIIHQM
                     GFDDYFLIVWDLLRFGRSQGYYMGMGRGSAVGSLVAYALNITGIDPVKHNLLFERFLN
                     AERYSMPDIDIDIPDIHRADFIRYVRDRYGSTHAAQIVTYSTFGAKQAIRDVFKRFGT
                     PEYELSNITKKISFRDSLTSAYQRNAAFRQIINSKIEYQKAFAIAQQIEGQPRQTSIH
                     AAGIVMSDEELTDTIPLKIGDDMLVTQYDAHAVEANGLLKMDFLGLRNLTFVQKMAEG
                     VRERYQQEIDIAKIDLEDKKTLELFAAGRTKGIFQFEQPGAISLLRRVKPGRFEDIVA
                     TTSLNRPGASDYSDNFVKRKHGQETVDLLDPTIAPILQPTYGIMLYQEQVMQIAQVYA
                     GFTLGKADLLRRAMSKKNALEMQAMEAEFLQGALKNGHAEENAREIFGMMAKFAGYGF
                     NRSHAYAYSALAFQMAYFKSHYTDVFFDVMLNHSSSAYIEDAMQFDFEVARLTINNIP
                     YHDKFDKSKVYLGLKTLKGLPKDLALWILDHRPYKSVEDFILRLPDNFKKKDLLIPLI
                     QIGLFDDFDANRKKIIENLDNLFVFAEAIGTFFAEESYSWTDVEDYSDSEKFMLEQEL
                     LGVGISPHPLVNICRESKRSFTDLADLAVGNRATVLVQIQAIRLIRTKKTGEQMAFLQ
                     VTDTKRTLDVTLFPESYRRYASILEEGGMGYITGRVQERDGQLQLVLEKLEPVSLEKF
                     WILLENREHDYAVARILDKYRGSIPVVLHYQDSNQTIQAEHHMVMKTPQLEEELSEFV
                     MKTIFR"
     gene            6078..7088
                     /gene="pfkA"
                     /locus_tag="VCA20_001544"
     CDS             6078..7088
                     /gene="pfkA"
                     /locus_tag="VCA20_001544"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEP1835348.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVYGINEGYAGM
                     VAGDIHELSARSVGDIISRGGTFLGSARYPEFAKLEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAIGVPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSH
                     RRTFVIEVMGRHAGDIALWAGIASGADVIVVPEEDFNINDVVDRIKAGYDNGKKHSII
                     VLAEGVMPAAQFAEELKVAGNTSDLRVTELGHIQRGGSPTARDRVLASRMGAHAVKLL
                     KEGRGGLAVGIRNEQMVENPILGTAEEGALFSLTTDGKIVVNNPHKADLELADLNRNL
                     SI"
     gene            7155..8660
                     /gene="pyk"
                     /locus_tag="VCA20_001545"
     CDS             7155..8660
                     /gene="pyk"
                     /locus_tag="VCA20_001545"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEP1835349.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKFGDDGYWGEKLDVESSAQKIAA
                     LITEGANVFRFNFSHGDHQEQGDRMATVRRAEEIAGKKVGFLLDTKGPEIRTELFEDD
                     AKEYAYTTGDKLHVATKQGIKSTKEVIALNVAGGLDVFDDVEVGKQILVDDGKLGLTV
                     VEKDAVNREFVVLVENDGVIAKQKGVNIPYTKIPFPALAERDNADIRFGLEQGLNFIA
                     ISFVRTAKDVEVVRNICKETGNDHVQLFAKIENQQGIDNIDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIITKVNAAGKAVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPVESVRTMATIAKNAQTLLNEYGRLNSDQFNRASKTEVIASAVK
                     DACHSMDIKLVVTVTETGYSARSISKYRPDADILAVTFTEKVQKSLMLNWGVIPIVTN
                     RPDSTDDMFDLAERIAKEQGLVEAGDDIIIVAGVPVGVAGSTNTMRVRTVK"
     gene            8824..12002
                     /locus_tag="VCA20_001546"
                     /pseudo
     CDS             8824..12002
                     /locus_tag="VCA20_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909520.1"
                     /note="This family includes IgM or IgG-cleaving cysteine
                     proteases.; frameshifted; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IdeS/Mac family cysteine endopeptidase"
BASE COUNT         3844 a         2134 c         2672 g         3532 t
ORIGIN      
        1 gcgcggagca gggacataga ctgttacccc ttcctcctta ctcaccagag tataatccta
       61 tcgagaaaac atgggctcac atcaaaaaac acctcagaag agtattgcca aattgcgata
      121 cgtttcttga ggtactttcg tcctgctctt gtttcagttg actatagttg gactaaacag
      181 ggggcaggaa gattttgaag tggttgctaa acttctgggt tgtggtttaa agcaaacgaa
      241 tacgaatcaa acgaaggaac tctttttggc tattgctgat ttggcaccaa aaggacttgt
      301 gggatttgat ttcgcaggaa atgaagcaga ctatcctacg gaagaacttc gggatattat
      361 tcaatttact cagtctttag gttatcccat gactttccat gccggagaat gtggatgtgt
      421 gaccaatgtg attcaagctc ttgaacttgg gattagaagg attggtcatg gcacagcact
      481 tactcgcaat cctgaagcta tccaagcttt tgtcaatagc ggagcaacac ttgaaatgtg
      541 tctaacatcg aatcttcaaa ctggtgcagc tgattcgatt gaatattttc catatcgtga
      601 gctagttgag gcaggagcaa acatcacaat caacacagat aatcgtaccg tttctaatac
      661 tacacttaat agagaatatc agttatttgt agaatatttt gggactagca aggctgattt
      721 ttatcgcttc aatcaaaatg ctattcaggc cagttttgcc agcgaagaag aaaagaaagt
      781 tttgttggaa ctcctagacc aacagtatta agaaaaaaac actataccgg tcgattagac
      841 cagtatagag ttttttattg tagatttaat ttctttgtca taatgaagtg ggttccaaag
      901 tagtaagcaa aagctaggat aatattgact aatgctaaga ctggacttgg atagagagaa
      961 aatggtgtag aataagctgt tcctaggtta cttaggacaa agaaagtgct aaatactcca
     1021 gtaacgatga gaatgccgat ataagtcaag attgcaaaga ggaagcgatg atccttgaat
     1081 aattgaccga cagatataga gaagtagatc aaaaggatac cagtggtaat ttcaattaaa
     1141 taatagacaa tctgactgaa tagaactgtc cagtcgatag cttgaacaaa agtttgtatt
     1201 tctggcataa tagcttcttc gcttgctgct agaatgactg caaggatgat tccaattgaa
     1261 agtaagcaag taatacctgc caagatatac caaacaatag aggcaagtag tttgcttaag
     1321 atgatctgat ggctagatac cggcagggtc atagtgagat aaccttgtcg gccataaaca
     1381 ttttttctaa aacggttgat tactagaaca aatgttgaca agaagagtgc ggcaatcaaa
     1441 ataccaaaag tcataaagga tgttcccaat aagaccatct ctccaccgcc aggttcagta
     1501 tttccagcct gtgtacggag ggccaacgct tgtagccaaa aaccaagtac tgctgatagg
     1561 accacagctg caatataaat tcccaaatac catttaccta cggattgaaa ttcgtatttc
     1621 aataattttt taaacatagc aatctccttc taactaagcc ttaaaatctt ggcggaagag
     1681 ttcgtcaata gattctccgc tttcaattcg caagtcgtct acattcccat ggcgaataac
     1741 acttccgtag tgtaggaaaa taacctcgtc caaaatttgc tcaacatctg aaattaagtg
     1801 agtagagata ataacagaag cagtcggaga gtagttgttg acgatggttt ttaaaatgta
     1861 gtctcgggcg gcagggtcaa caccaccaat tggctcatct aaaacataca gctgtgcttg
     1921 acggctcatc accagaatga gctggacttt ttccttattc cccttggaga gatgtttcat
     1981 acgactgttt aaatcaatgc ccaaatcttg taaaagatgg agagcacgct ccttatcaaa
     2041 atcagcgtaa aaatcttcaa aaaatgtaat tgcttcgagc actttcatat gttcatcaag
     2101 ataagtggta tctgggagat aagatacaat cgccttggtt tctggcgatg gtgtgcgacc
     2161 attgatcaat acttgaccat attctggttg caacagaccg ttgatcaatt taatcatggt
     2221 tgttttaccc gaaccatttg gtcccaaaag tcctatgatt tttccggcag ctaacttcaa
     2281 attaacatta tttagggcga cagctccacc ataagatttt gaaacttgct gtagctcaac
     2341 caaagtatat gcgttactca taagtaactc ctttcaaata atcatctaac tggctagtga
     2401 ggtcgtcgtg tttgaacccc aaatctaaca tattgttcac gaagttctcc agttcctttt
     2461 gggctaaatg taggcgagtt tgtgtaatca attcttgatt atctgttaca aaacggcctg
     2521 ttgtgcgtac tgagtaaaca aatccttctg tttctaaatc agacaaagcg cgctgaacgg
     2581 tatttggatt gacaccagca atgtcggcta agtcacgaac agttggaagt ttatctccag
     2641 attttaattg attggtcaca atctgcaatt taatagtatt gctgatttga atatagatgg
     2701 ggatgttatt atcaaatttc caagccataa tcgtttcctt tctaggatga cacccgtgtg
     2761 tatgtcttat attaataata caataaatca tataaattag caagggaaat gttttgaatt
     2821 tttaaaaaat atttttggat aaatatttga tataatagaa gaaacatgaa aaggagaata
     2881 ccatgctggc acaattagat acaaagacag tttatacctt tatggatagc atggtgacga
     2941 tagaaaatta tgtaggcaag gctaaagagc tcggttatca cgcattgggg ataatggata
     3001 aaaacagtct ttatgcagcc tatagtttta tggaatcctg taacaaaatg gggcttcaac
     3061 caattattgg ttgtgaactg gactggaagc tacggggagg ggagcaggat gtcactactc
     3121 aattgatcgc tctgacaacc agaggttatc gtaacctatt gaaaatttcg actgctaaga
     3181 tgacagggca agatgatttt gaaactattc gtcaatattt agtcgacata gcagtggttg
     3241 ttccttattt ttctggcatt gagcacctcg atttgggtgt aaactattat atcggagtga
     3301 cagttgacac accggttcaa gattttaagc aagcacccct tccaatccat actgttcgtt
     3361 tttttgaaga ggaacaattg gaaagtctac aggttctcca tgctattcga gataatattc
     3421 cgcttaatca ggtttcagat gtagatagtc ggcaaaagct tttatcggct gatagcttaa
     3481 ccaatctatt ttctaatcgg tttccgcaag cagttgataa tttacgtgac ttggttgcag
     3541 ggatttctta tcaattaaat acagatttaa aattgccacg ttttaatcgt gacagacctg
     3601 cagtggaaga attatacgag cgctcctatc aaggattaga agagaagggc ttgactaatt
     3661 cactgtataa ggaacgcttg gaaaaggaat tatccattat tcatcaaatg ggatttgatg
     3721 attatttctt gattgtatgg gacctacttc gttttggacg tagccaagga tactacatgg
     3781 gaatggggcg cggttcagct gttggaagcc tagttgccta cgccttaaat atcacaggta
     3841 ttgatcctgt taagcacaat cttctctttg aacgctttct caatgctgaa cgttattcaa
     3901 tgcccgatat tgatattgat attccagata ttcatcgagc agattttatt cgttatgtac
     3961 gagatcgtta tggttcaacc catgctgcgc aaattgtaac ctactcgaca tttggagcta
     4021 agcaggccat tcgtgatgta tttaagcgtt tcggaacacc tgaatatgag ttaagtaata
     4081 ttaccaagaa aatttcattt agggatagtc ttacttctgc ctatcagcga aatgccgctt
     4141 ttcgtcagat tatcaatagt aagatagagt accaaaaagc ttttgcaatt gctcaacaaa
     4201 ttgaagggca accccgacag acttctatcc atgcagcagg gatagtgatg agtgatgagg
     4261 agttgacaga taccatccct cttaaaatag gggatgacat gctggtaacc caatatgatg
     4321 cacatgctgt tgaggcgaat ggtctgttga agatggattt ccttggctta cgaaatctga
     4381 cctttgtgca aaaaatggca gaaggagtac gtgaacgtta tcagcaggaa attgatattg
     4441 ccaagataga tttagaagac aagaaaacct tggaactttt tgctgctggt cggactaagg
     4501 gaatttttca atttgaacag cctggtgcga ttagcctgct tcgaagggtc aaacctggtc
     4561 gatttgagga tatagttgca acaacgagtt tgaatagacc gggtgctagt gattattcgg
     4621 ataactttgt aaaacgcaaa catggacaag agacagttga tttgttggac ccaaccattg
     4681 ctcctatttt acaaccgact tatggtatca tgctttatca ggaacaggtc atgcagattg
     4741 cacaggtcta tgcaggcttt acgcttggaa aggctgattt actccgtagg gctatgtcta
     4801 aaaagaatgc cctggaaatg caggcaatgg aagcggaatt tttacaggga gcactgaaaa
     4861 acggacatgc tgaagagaat gcaagggaaa tatttggcat gatggctaag ttcgctggtt
     4921 atggttttaa ccgtagccac gcctatgctt attcagcctt agcctttcaa atggcctatt
     4981 tcaagagtca ctatacggat gtcttttttg atgtcatgct caatcattct agtagtgctt
     5041 atattgaaga tgcgatgcag tttgactttg aggtagctcg actcacgatt aacaatattc
     5101 cttatcacga taagtttgat aaaagtaagg tctacctagg attaaaaaca ctaaaaggtt
     5161 tacctaaaga tttagcttta tggatattgg atcatcgtcc ctataagtct gtagaagatt
     5221 ttattttgcg cttgccagat aatttcaaga aaaaagattt actaattcct cttattcaga
     5281 ttggtttatt tgatgatttt gacgccaatc gtaaaaagat tattgaaaat ctagacaatc
     5341 tctttgtctt tgcagaggcg attggtacat tttttgcaga ggagagttat agttggacag
     5401 atgtagaaga ctacagcgac agcgaaaaat ttatgctaga gcaagaattg ctaggtgttg
     5461 gtattagtcc gcatccttta gtcaatattt gtcgagagtc taagcgtagt tttacagatt
     5521 tggctgattt agcggttggc aatcgagcga ccgttcttgt gcagattcaa gctattcgat
     5581 tgattcgtac caaaaagact ggggagcaga tggcatttct tcaagtgaca gataccaaac
     5641 ggacgcttga cgtgactctt ttcccagaat cttatcgccg atacgcttct attttggaag
     5701 aagggggaat ggggtacatc acaggtagag tgcaagagcg cgatggacag ttgcaattag
     5761 ttctagaaaa attagagcct gtcagtttgg agaaattttg gattcttttg gaaaatcgag
     5821 aacacgatta tgccgttgct cgtattttgg ataaatatag aggaagcatt ccagtagtac
     5881 ttcattatca agatagtaat cagacaattc aggctgagca ccacatggtt atgaaaacgc
     5941 ctcaattaga agaagagtta agtgaatttg ttatgaaaac gatttttcga taaaaaaata
     6001 gcaaaaagtc aagcaagttt aggttaagta tgttataata gggcagttaa aatgaaaagt
     6061 taaaggagca ttaacgaatg aaacgtattg ctgttttgac cagtggtggt gacgcccctg
     6121 gtatgaacgc tgctattcgt gcagttgttc gtcaagcaat ttcagaagga atggaagttt
     6181 atggaatcaa cgaaggttac gcaggtatgg ttgcgggtga tatccatgaa ttgtctgcac
     6241 gttctgtagg tgatattatt tcacgtggag gaacattcct tggttcagct cgttatccag
     6301 aatttgccaa attagaaggg caacttaaag ggattgagca attgaaaaaa catggtatcg
     6361 aaggtgtagt tgttatcggt ggtgacggtt cttaccacgg agcaatgcgc ttgacggagc
     6421 atggtttccc agctattggt gtacctggta ccattgacaa cgacatcgtg gggacagatt
     6481 ttacaatcgg ttttgacaca gctgtaacaa ctgccatgga tgcgattgat aagattcgcg
     6541 atacttcatc aagtcaccgt cgtactttcg tcattgaagt aatgggacgt catgcaggtg
     6601 atatcgctct ttgggctggt attgcttctg gtgcagatgt tatcgttgtt ccagaagaag
     6661 acttcaatat caacgatgtt gttgaccgta ttaaagcggg ctacgacaat ggtaagaaac
     6721 atagcatcat tgttttggct gaaggtgtta tgccagcggc acagtttgct gaagaattga
     6781 aagtagcagg caatacaagt gatcttcgtg tgacagaact tggtcatatc caacgtggtg
     6841 gttctccaac agcgcgtgat cgtgtactcg cttctcgtat gggtgctcat gccgttaaat
     6901 tgcttaaaga aggccgtgga ggtcttgcag taggtatccg taacgagcaa atggttgaaa
     6961 atccaattct tggaactgca gaagaaggtg ccttgtttag cttgactact gatggcaaga
     7021 ttgtagttaa taatccgcat aaagcagatt tggagttggc tgatttgaac cgtaacttgt
     7081 caatctaggt atcattgtct aaaagtgtta attaagggtc atatgaccat tgaaataaaa
     7141 ggagttttgt taagatgaac aaacgtgtta aaattgttgc aacccttggt ccagcggtag
     7201 aaatccgcgg tggtaaaaaa ttcggtgacg atggttactg gggtgaaaaa ttagatgttg
     7261 agtcatcagc acagaaaatt gctgctttga ttacagaagg tgccaatgtt ttccgtttca
     7321 acttctcaca cggtgaccac caagagcaag gtgatcgtat ggcaacagtt cgtcgtgcag
     7381 aagaaatcgc tggtaaaaaa gtaggtttct tgttggatac aaaaggtcca gaaatccgta
     7441 cagaattgtt tgaagatgat gcaaaagaat acgcatatac aactggtgac aaattgcatg
     7501 ttgctacaaa acaaggtatc aaatctacaa aagaagtgat tgctttgaac gtagcaggtg
     7561 gtttggatgt ctttgacgat gttgaagttg gtaagcaaat ccttgttgac gatggtaaac
     7621 ttggtttgac agttgttgaa aaagatgcag taaaccgtga atttgttgtt ttggttgaaa
     7681 acgacggtgt aattgctaaa caaaaaggtg taaacatccc ttacacaaaa attcctttcc
     7741 cagctcttgc agagcgtgat aatgcagata tccgcttcgg tcttgagcaa ggtttgaact
     7801 ttattgctat ctcatttgta cgtactgcaa aagacgtaga agttgttcgt aacatctgta
     7861 aagaaactgg taacgaccac gttcaattgt ttgcgaaaat cgaaaaccaa caaggtatcg
     7921 acaacatcga cgaaatcatc gaagcagcag atggtatcat gattgctcgt ggtgacatgg
     7981 gtatcgaagt accattcgaa atggttccag tttaccaaaa aatgattatc actaaagtga
     8041 atgcagctgg taaagcagtt atcacagcaa caaacatgtt ggaaacaatg actgaaaaac
     8101 cacgtgcaac tcgttctgaa gtatctgacg tattcaatgc tgttatcgac ggtactgatg
     8161 caacaatgct ttcaggcgag tctgcaaacg gtaaatatcc agtagaatca gttcgtacaa
     8221 tggcaactat cgctaaaaac gcacagactc ttcttaatga atatggtcgt ttgaactctg
     8281 atcagtttaa tcgtgcctct aaaacagaag ttattgcttc tgcagtaaaa gacgcatgtc
     8341 actcaatgga catcaaacta gttgtaacag ttacagaaac aggttactca gctcgttcga
     8401 tctctaaata ccgtccagat gcggacatct tggcagtaac atttactgag aaagtacaaa
     8461 aatcattgat gcttaactgg ggtgttattc caattgtaac aaaccgtcca gattcaactg
     8521 acgatatgtt tgatttggct gaacgtattg ccaaagagca aggtcttgtt gaagcaggag
     8581 atgacattat tatcgttgct ggtgtgcctg taggtgttgc aggttcaaca aatactatgc
     8641 gtgttcgtac agtaaaataa taaaaaggtc cgcttggacc tttttgtcgt gattatggaa
     8701 atagactata tctgaatttc tttcaaacgc ttgtttagaa atattttctt gggggatgtg
     8761 gaaataaaaa taagtcgtaa atagaatagt ttatttaaat ttttgatgat aaacggagga
     8821 aagatgaaca ttcaagaacg attttctttg agaaaatcag cggttggctt ggtctaagtc
     8881 tctttgctat gtgctattta tacatccact gttgctgcag atacagttgt tacaggaatg
     8941 aatgaaataa ttgaagaatc acaagtcaag gatgaggtat ctattgaacc agaaaaaatg
     9001 aatccctaga tagttctaat cttgaaattg tagaggaaat agcagacaac atcccatcac
     9061 ctgttatcgc tgaaggggaa gtagcggtag agatgaactc ggacaatggg gacgagaatg
     9121 tagtttctag agacgactca gaagttacga caaatgagca aaatcagata gaggttactg
     9181 agacaaaaga aattttgaat tacaccagct accaaacgga gagtggcgag caacgacaaa
     9241 ttgtatgggc ctatggaatt actcctcctg caatggaaca gagcggtggt tttgtaaagg
     9301 aaaagtatgg agactattta aactatacag caccgtttga ggctggaaaa ggctactatg
     9361 ataccaataa gagtctgaat gcttcattta ttgaccttaa tctttgtttt gcagctgtgt
     9421 cttcaaacat ggtacattgg tggttggaac agaatagttc ctatgttgag cgatatctca
     9481 aagaaaaaaa gggtacagta aatgttgaag aaaactacgc aataacggac ttacggcgct
     9541 atattaattc attccaaaat caacaaaata gtcgagtttt tgatatgttc aaaacttact
     9601 atggttatcg tacaaatggt tttgtatcag atgccttggt tgacttgttt attaacggat
     9661 ataaacctaa ggcacagggc ggtgtcaatc tggaagatag ccagttagta cccgatagta
     9721 ggggtggctt tttctacgac gttttcaaag agaaaaaact gacaaatcga atttttagtg
     9781 gtagttatga gcggtttggt gaggatgttc gaactgtttt ggaaagcaaa ggattactcg
     9841 gcttaactta tagaacatta ggttatgcaa tgcatattgt gacggtatgg ggtgctgagt
     9901 acgacaatca aggtaagatt aaggctgtct atatcacaga ttctgatgat caacaagaac
     9961 aaattggttt gaagcgtatg ggaatcactc gtgatgcttc cggaaatcca cgtttgaata
    10021 atcatatgaa aaataattca gctggagcgc ttttggatta tgtccataca atccgtctgg
    10081 gtcaagactt atgggaagaa tatttcaatc cgcttgcaaa agccaaagaa acagctagtc
    10141 agacattagc cgatacaaag aaggcgttgg atttgtctat tcaaggacaa tctgaattgc
    10201 cagaatcaat gcgactgatt tatcttgaaa aactaaataa tctctataat caaggaattc
    10261 tatctattca aaaggcagaa agttctgaga tgctaagtgg tgcattggaa aatggtttaa
    10321 atagtttaaa gagtttagat tttcctattt cagaagttgg aaatgctttg gcaccagatt
    10381 taccagtagg tgatcgctca acggtttcag atgttgattc tctatcatct caagaaacaa
    10441 gttccacaaa tttgtaagta gacacagaga atgcaggtct tattgcagat ggtgccgatc
    10501 aattgcattt tccagtggag gtccaaacga catcttttgt agaggctgag ggagataatg
    10561 tttttgaaca agaggcagat acattgccaa taattattga aaacaaggat gaatttggtt
    10621 cagaactatc aagaaacatg caaacgtcag aaacggattc gctagtagta gctgttgaag
    10681 aagatgtgaa aaatgatgag gtagcccaag ttgaagaact tcttgagtca gaaaaagttg
    10741 aaaatcagag ttcggaactt ctgtcagaca ccctaatcgt agagggtgca aatgacaaag
    10801 aagaagatag agtggaggcg gttgtttctg aacaaccaga ctcaatacca catcaaaatg
    10861 tagaaatctc tcttgtagaa ccaacgaatg tcgaaactga atctgtggtc actcctacta
    10921 atgatgtagc tactcctcat ggttccccga cgtatataga taattccgta actgaatctg
    10981 tagctactcc acttgaaaaa gactccattc aagccggaga gacagaaaat gcagaaccaa
    11041 cctcgagcga atcaactagt gtcgaagctg aacttgtcga caattctgaa attcattcag
    11101 ctacctcttc agttactccc cgtggttcct cggcatatgc agatagttct acaactgaat
    11161 ctgtagccac tccgcttgaa aaaaattcca ttcagaccag agagatagaa aatgcggaac
    11221 aagctttgag cgaaccaacg aatgccgaag ctgaatctgt agctactccg cttgaaaaag
    11281 actccgttca ggctggagag acagaaattg cagaaccaac ctcgagcaaa tcaactaatg
    11341 tcgaagctgc atctgtcgac aattctgaaa ttcatgcaga tgcctctcta actgctgttt
    11401 catcagtcaa tctggataat ccagtgattg aaccagtagc tatccccctt atcggttcta
    11461 agagggacac gaatgcagaa gtggaagttt cttcattatc gaaaagagtg gttagaaaac
    11521 caaatactga agggctaatc tctgttcaat caaaaattat taagaaagaa ttgctagaat
    11581 caagcttagc agaagcaggg tctccattgc tagaagccac cattgctcag tcttcaaact
    11641 caaatagtac tgagataggt atgagctatc agaatactgt gttattagag tctaataata
    11701 cagagcgtca agcgtctaaa gcagaaattg ttatggaaca caaggagaca gagttagttg
    11761 aaacggtttc atctgcttct gagcctgtag tgctcgtaga aaatatctca caaacctcaa
    11821 ataatactat tgaatctggt aagaatatgg gagttcaatc tcaagcaggt gcaaaacaaa
    11881 ttttaggcgt agaacaatct tcgaaagtaa gtacacctac ttcaagacag attatgggag
    11941 tctgtctatt gactcttgtt cttggtagtg ctttaggttt gttaaagaaa agacgtaagt
    12001 aaaagaagta tcgtcattta gtttttcttt tattacttcg ttaactcgcc ttacctaact
    12061 ccagttatgc ctgcggctcg ttgcctagta ctaaaagtaa actaaaagac tatattttca
    12121 acgaattagg tttttaagtg aatcagtttc tatggttgcg ataacatgct atagatatag
    12181 tt
//
LOCUS       DATUCG010000074        12055 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.075, whole genome shotgun sequence.
ACCESSION   DATUCG010000074 DATUCG010000000
VERSION     DATUCG010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12055
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.075"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            108..1697
                     /locus_tag="VCA20_001547"
     CDS             108..1697
                     /locus_tag="VCA20_001547"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEP1835350.1"
                     /translation="MSKTIQAITNQIWSMANELRGNMDASEYKNYILAFMFYRYLSEH
                     QERFLVEDEIISPAPGETVQDAYAREAVGNDLVEHLENTASHLGYAIEPEDTWARLVA
                     KIDNSEIVASDYQTIFDHFNANVELNRDAMEDFRGVFNDINLGDSRLGNSTVARAKSL
                     NSIVKLIDSIEYKNDEGKDILGEIYEYLIGQFAASAGKKGGEFYTPHQVSKILAKIVT
                     LGLEKSDTSFSVYDPTMGSGSLLLTVRNELPQGQHIKFYGQEMNTTTYNLARMNLMMH
                     QVGYSNMILNNADTLESDWPDGVDELGINQPRSFDAVVANPPYSAKWDNRESKLKDPR
                     FMEYGKLAPASKADFAFILHSLYHLNNTGTMAIVLPHGVLFRGAAEGHIRKLIIEKNY
                     LDAVIGLPANLFYGTGIPTTILVFKKNRQTKDVFFIDASKEFEKGKNQNHLSDDMVEK
                     IVETYHNRQSVDKYAHLASIEEIVENDYNLNIPRYVDTFEEEEEIDLGQVTQQLEQDR
                     LEIRALEDKIRQQLKTLGVDF"
     gene            complement(1748..3022)
                     /gene="serS"
                     /locus_tag="VCA20_001548"
     CDS             complement(1748..3022)
                     /gene="serS"
                     /locus_tag="VCA20_001548"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEP1835351.1"
                     /translation="MLDIKRIRTDFDGVAAKLATRGVGAETLADIKELDAKRREILVT
                     VEELKAERNTVSAEIAQAKRNKENADDKIAAMQELSADVKNLDAELLEIDEKLNAILA
                     VLPNTPHDSVPVGADEEENVEVRRWGTPREFTFEPKAHWDLGEDLDILDWERGAKVTG
                     ARFLFYKNLGARLERALYNFMLDEHIAEGYQEIITPYMVNHDSMYGTGQYPKFKEDTF
                     ELADTNFVLIPTAEVPLTNYYRNEILEEKDLPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFATPEQSYDELEKMTANAENILQKLGLPYRVLSLCTGDMGFSAAKTY
                     DLEVWIPAQNAYREISSCSNTEDFQARRAHIRYRDAADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEVLRPYMGGVEVIKS"
     gene            complement(3241..3612)
                     /locus_tag="VCA20_001549"
     CDS             complement(3241..3612)
                     /locus_tag="VCA20_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003092275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEP1835352.1"
                     /translation="MAQSQNTSVELQTTGVSYMGFGGKIGKFLIGDKALEFYPDSNVE
                     RYIQIPWSEMTSIGANVSGKAISRHFEIYTEKSRFLFASKDSGKILKIAREQIGNEKV
                     VKLPTLMQTIGRKISNLFAKK"
     gene            complement(3671..4582)
                     /locus_tag="VCA20_001550"
     CDS             complement(3671..4582)
                     /locus_tag="VCA20_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEP1835353.1"
                     /translation="MSEKIQLSVSDRKKVWWRSTFLQGSWNYERMQNLGWAYAMIPAI
                     KKLYTKKEDQAAALERHLEFFNTHPYVASPILGVTLALEEERANGAEIDDTAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGALGASLALSGNIMGPIIFFLGWNIIRMAFLWYTQE
                     FGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVSINFIVDLPAKQLSEGA
                     YIVFPEGTVTGTELQGILGDALSGLSLYPTQAQTLQGQLDSLIPGLMGLLLTFLCMHL
                     LKKKVTPITMIIGLFIVGILARFFNIM"
     gene            complement(4600..5409)
                     /locus_tag="VCA20_001551"
     CDS             complement(4600..5409)
                     /locus_tag="VCA20_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEP1835354.1"
                     /translation="MSDITLISAILVVIVAFFAGLEGILDEFQFHQPLVACTLIGLVT
                     GNLAAGVMLGGSLQMIALGWANIGAAVAPDAALASVAAAIIMIKGGDFSTEGIAVATT
                     TAIPLAVAGLFLTMIVRTISVGLVHGADNAAKDANFAAVERYHLFALLLQGLRIAVPA
                     ALLLAIPAEAVQSVLEAMPAWLSGGMAVGGGMVVAVGYAMVINMMATREVWPFFALGF
                     AFAAISQLTLIALGVIGVAIALIYLNLSKQGGSGNGGASSNDPIGDILEDY"
     gene            complement(5441..6433)
                     /gene="manX"
                     /locus_tag="VCA20_001552"
     CDS             complement(5441..6433)
                     /gene="manX"
                     /locus_tag="VCA20_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEP1835355.1"
                     /translation="MSIGIIIASHGEFAAGIHQSGSMIFGEQEKVQVVTFMPSEGPDD
                     LYAKLNAAVDAFDADDEVLVLADLWSGSPFNQASRVAGENPDRKFAIITGLNLPMLIQ
                     AYTERMINAAAGVEEVAANIIKEAKDGIKVLPEELQPAEATPVADAAPVALQGAIPPG
                     TVIGDGKLKINLARIDTRLLHGQVATGWTPASKADRIIVASDTVAKDELRKQLIKQAT
                     PGNVKANVVPIKKLIEVSKDPRFGNTHALILFETPQEALEAIEGGVEIKELNVGSMAH
                     STGKTMVNNVLSMDKDDVATFEKLRDLGVEFDVRKVPNDSKKDLFELINKANVQ"
     gene            complement(6654..7466)
                     /locus_tag="VCA20_001553"
     CDS             complement(6654..7466)
                     /locus_tag="VCA20_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEP1835356.1"
                     /translation="MKKKIIAIDLDGTLLNNDSQLSDYTISTVKKVRQAGHTVLIATG
                     RPYRMAEKFYQQLELDTPMINFNGSLVHIPGKKWQWEQNILIDKKYLLEFLKEEETFE
                     ADFIAGEYKNKFYITQKNLDKIDPALMGVEQITPDTLIKPELITSDPHSILMQTRATD
                     KYELAKEMNAYFKDELEINTWGGPLNILETCAKGVNKATALSYVLNLYQASPSDLIAF
                     GDEHNDVEMLDLAGTAYAMKNCSDTLRPHADRLTAFANFEDGVARELENLFL"
     gene            7811..9271
                     /locus_tag="VCA20_001554"
     CDS             7811..9271
                     /locus_tag="VCA20_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEP1835357.1"
                     /translation="MDQFFKLKEHGTTVSTEIMAGLTTFFAMSYILFVNPSVLSVAGM
                     PTQAVFLATIIASAVSTLVMGLFANVPYALAPGMGLNAFFTYTVVIGLGFTWQEALAM
                     VFLCGLFNVFITVTKVRKSIIKAIPVSLQHAIGGGIGVFVAYLGFKNSNLITFLTSGS
                     DIITVNGVAPADATAETFSNGVFSVFAGGGVVPGISTFTDPSVLLTVFGLLLTAVLVI
                     KNVRGAILIGIIATTVAGIPAGVVDLSTIDFANNNIGTAFAELGTTFLAAFDGLSSLF
                     ADSSRLPLVLMTIFAFSLSDTFDTLGTFIGTGRKTGIFSEEDEKALENGTGFNSKMDK
                     ALFADAIGTSIGALFGTSNTTTYVESAAGISAGGRTGLTAVTTAILFLLSILILPFIG
                     IVPNAATAPALIIVGVMMVSSFLDVDWSQFEDALPAFFAAFFMALCFSISYGIAAAVI
                     FYCLVKVSTGKAKEIHPILWGATALFILNFIILAIL"
     gene            complement(9609..10469)
                     /gene="metF"
                     /locus_tag="VCA20_001555"
     CDS             complement(9609..10469)
                     /gene="metF"
                     /locus_tag="VCA20_001555"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEP1835358.1"
                     /translation="MIGQTPSLSFEIFPPKPEVGNEKIIQTLDEMQGLVPHFISVTCS
                     NNNLNVEDTTVKLANHVRNELHIPTIAHLLAAYLTKEKVRSVLHSLDEIGVHQILVLR
                     GDIIEALPPKEDFQYATDLISFIKEEAPHFDIIGACYPEVHPESPNSVSDIKNLKKKV
                     DAGCSTLVTQLFFDNEAFYNFQEKCSLADIEVPIIAGIMPIINRNQALRLLKTCENIH
                     LPRKFKAILEKYEHNPESLRAAGLAYAVDQIVDLVTNDVAGIHLYTMNNAETARTIHQ
                     ATHSLFKHYS"
     gene            complement(10473..>12055)
                     /gene="metE"
                     /locus_tag="VCA20_001556"
     CDS             complement(10473..>12055)
                     /gene="metE"
                     /locus_tag="VCA20_001556"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEP1835359.1"
                     /translation="KDTEIKLAGHKIFDEFQEAKNLGITTRPVLIGPFTLLQLTDFEE
                     GKETATLVATGFPADKTLYAGIVNGKNIWRNNYEKSLAVLDTIPAENVVITTSCSLLH
                     VPFTTANEEFEPAILNHFAFAVEKLSELRDLDAIRNGQGEVALTANKELFALERVGRD
                     AALADRLAGLTDSDYTRLPVFAEREATQREKLNLPLLPTTTIGSFPQTKEVRSTRLAF
                     RKGNISEEEYDAFVKTQTDEWIAWQEEVDFDVLVHGEFERNDMVEYFGENLSGYLFSK
                     NGWVQSYGMRGVKPPIIWGDVTRLNPITVKWSSYAQSRTNKPVKGMLTGPVTILNWSF
                     PREDISIKESTLQIALAIKEEVLDLEAAGIKIIQIDEAALREKLPLRRSDWYSEYLDW
                     AIPAFRLVHSTVAPDTQIHTHMCYGEFTDIIPAIDNMDADVISFEASRSNLVILDELK
                     AKNFQTQVGPDVYDIHSPRVPAVDEIAHTIQAILAKVPKEKVWINPDCGLKTRGESET
                     KASLIHLTQAAKTARKEL"
BASE COUNT         3351 a         2380 c         2667 g         3657 t
ORIGIN      
        1 gaagccatcg gcagcttctt ctccgaccta gatcagctta ttactcttca tcagcgaaaa
       61 tgagatttcg agtaaaaaat gaaacaacat gaaaggatta caaaaacatg tcaaaaacta
      121 ttcaggccat taccaatcag atttggtcca tggccaatga attgcgagga aatatggatg
      181 cttcggaata taagaactat atcctagcct tcatgttcta tcgctatcta tctgagcatc
      241 aagagcgttt cttggtggaa gatgaaatta tcagtcctgc tccaggagaa actgttcagg
      301 atgcctatgc gcgagaagca gttgggaatg acttggtaga gcatttggaa aatacagctt
      361 ctcaccttgg gtatgccatc gagcctgaag atacctgggc aagactggta gccaaaattg
      421 ataacagtga gattgtagcg agtgactacc agacgatttt cgatcatttt aacgccaatg
      481 tagaactcaa tcgggatgct atggaagatt tcagaggcgt ctttaatgac atcaatctag
      541 gggactctcg tttgggcaat tcaacggttg cgcgtgcaaa atccttgaac agtattgtga
      601 agttgattga tagcattgag tataaaaatg acgaaggaaa ggatattcta ggagaaatct
      661 atgagtatct tattggacag tttgccgctt cggcaggtaa aaaaggtgga gaattttata
      721 ccccgcatca agtgagtaag attttagcca agattgttac tttaggtttg gaaaaatcag
      781 atactagctt ctctgtttac gatccaacca tgggttcagg gtctttgcta ctaacagtgc
      841 gtaatgaact acctcagggc cagcatatca aattctatgg ccaagaaatg aacacaacta
      901 cttataactt ggcgcggatg aacctgatga tgcatcaggt cggctacagc aatatgattt
      961 tgaataatgc tgatacctta gaaagtgact ggccggacgg ggtcgatgaa cttggaatca
     1021 atcagccacg tagttttgat gcggtcgtcg caaacccacc ctactctgct aaatgggaca
     1081 atcgtgagtc caaattgaag gatccacggt ttatggaata cggtaagcta gcaccagcct
     1141 ctaaagcgga ctttgctttt atcttacaca gtctttatca tctgaataat acagggacca
     1201 tggccattgt tttaccacac ggagtgctct ttaggggagc agcagaaggt catattcgca
     1261 aactgattat tgagaaaaat tacctggatg cagttattgg cttgccagct aacctgtttt
     1321 atggcacagg cattccaacc actattctgg tcttcaaaaa aaatcgtcag accaaggatg
     1381 tcttctttat cgatgcgagc aaggagtttg aaaaagggaa gaaccagaat cacctttccg
     1441 atgatatggt agaaaaaatt gtagagacct atcacaatcg tcagtctgtt gacaaatatg
     1501 ctcatttggc atcaatagaa gaaattgtag aaaatgacta caatctaaat attcctcgtt
     1561 acgtcgatac ttttgaggaa gaggaagaga ttgatctcgg gcaagtaacg cagcaactag
     1621 aacaagatag actagagatt cgggctctag aagataagat tcgtcaacaa ttaaagactt
     1681 tgggagtaga tttttagata aatgaaaaga gcgagtagga tttttcctcg ctctttcttt
     1741 gtatgtctta agatttaatc acttccaccc cacccatgta tggacggaga acttctggaa
     1801 ttgtgacaga accatcttcg ttttggtagt tttcaaggat ggctgcaacg gtacgaccaa
     1861 ccgctagacc agaaccgttg agggtgtgga gcaatttaac cttgccgtca gcagcatcgc
     1921 ggtagcgtat gtgggcacgg cgggcttgga aatcttctgt gttggaacag cttgagattt
     1981 cacggtaggc attttgggct ggaatccaga cttccaagtc gtaggtctta gcagctgaga
     2041 agcccatgtc gcctgtgcag agggataaca cgcggtatgg caagcctaat ttttggagga
     2101 tgttctctgc gtttgcggtc attttttcca attcatcata agactgttct ggtgtggcaa
     2161 atttgaccat ttcaaccttg tggaattggt ggaggcggat cagaccacgg gtgtcacgac
     2221 cagcagaacc agcttcagaa cggaaagatg ggctcatggc tgtgaagtaa attggcaggt
     2281 ctttttcttc caaaatttcg ttgcggtagt agttggtcaa tggcacttcc gcagttggga
     2341 tgaggacgaa gttagtgtct gccaactcaa aagtatcttc cttgaactta ggatattgac
     2401 cggtaccata cattgagtca tggttaacca tgtaaggtgt gatgatttct tggtagcctt
     2461 ctgcgatatg ctcgtccaac atgaagttgt agagggcacg ttctaagcga gcacccaagt
     2521 tcttgtagaa gaggaagcgt gcaccagtta cttttgcgcc acgttcccag tccagaatat
     2581 ctaggtcttc gcccaaatcc cagtgagctt ttggctcgaa ggtaaattca cgaggcgtgc
     2641 cccagcggcg aacttccacg ttttcttctt catctgcacc aacagggaca gaatcgtgcg
     2701 gcgtatttgg aagaacagca agaatggcat ttagtttttc atcaatttcc aagagttcag
     2761 catctaaatt tttcacatct gcagacaatt cttgcatagc agcaatcttg tcgtcagcat
     2821 tttctttgtt gcgttttgct tgggcaatct ctgctgaaac tgtgttacgt tcagctttta
     2881 attcttctac agtaaccaag atttcacggc gttttgcatc caattccttg atgtctgcca
     2941 aggtttcggc acccacacca cgagtagcta atttggcagc cactccgtca aaatctgtac
     3001 gaatacgttt gatatctagc atagatgtct cctttagaat aaaaaaaaca cagaatgaga
     3061 gatgctggag cgatatttac cgcggttcca tccagcttca cattctgtgc acttgattgt
     3121 attttgataa tttcataacg gtagaatttc acaacttttt cctatctgct cacagcgacc
     3181 gcagactttc tggaaggagc cagatgctac ttatccgttt ctactattat acttgaattt
     3241 ttattttttg gcaaatagat tcgaaatttt tctgccgatt gtttgcataa gagtcggtaa
     3301 tttcacaact ttttcattgc cgatctgctc gcgggcaatt ttaagaatct taccagaatc
     3361 tttagatgca aacaagaatc gacttttctc tgtataaatt tcaaaatgac ggctgattgc
     3421 tttaccagaa acgtttgcgc caatgctagt catttctgac caaggaatct ggatatagcg
     3481 ttcaacgttg ctatctgggt agaattcaag tgccttgtca ccaattagaa atttaccaat
     3541 cttgccacca aagcccatgt aggaaacccc tgtggtttgt agttcgacgc ttgtattttg
     3601 tgattgtgcc atagtactcc tccctagata ttcattatat cataaaaccc cgcaagagcg
     3661 gggtccttga ttacataatg ttgaagaaac gagcaagaat acctacgatg aagagaccga
     3721 taatcattgt gattggtgtt actttcttct taagcaagtg catgcagagg aaagtaagga
     3781 gaagtcccat caaaccagga atcaagctgt ccaactgacc ttgtaatgtt tgtgcttgag
     3841 ttgggtaaag gctaagtccg ctgagagcat cacccaggat gccttgaagc tcagtacctg
     3901 ttacagtgcc ttcagggaag acgatgtaag caccttctga caattgtttt gcaggaagat
     3961 caacgatgaa gttgatagat acccagcgtt gaacaagaac ggcaaggatg aacataccaa
     4021 ggattgaagc acctttagtg atgtcttgaa ggataccacc agacatgtct ttagtgattt
     4081 cactaccagc tttgtaacca aattcttgtg tataccatag gaatgccata cgaatgatgt
     4141 tccagccaag gaagaagatg attggtccca tgatatttcc agatagtgca agtgaggcac
     4201 caagagcacc aaggattgga cgaacagtaa accagaagac tgggtcaccg ataccagcaa
     4261 gaggtcccat cataccgatt ttcacacctt ggatagctgt gtcatcaatt tcagcaccat
     4321 tagcacgctc ttcttcaagg gcaagagtta caccaaggat tggtgaagca acgtatgggt
     4381 gtgtgttgaa gaattccaag tggcgctcaa gagcagcagc ttgatcttct tttttagtgt
     4441 aaagtttttt gatagctggg atcattgcat aagcccatcc taagttttgc atacgctcat
     4501 agttccatga accttgaagg aaggttgaac gccaccaaac ttttttacga tcgcttacgg
     4561 aaagttgaat tttttctgac atgatgaatc tcccttctct tagtagtctt caaggatgtc
     4621 gccgattggg tcgttagatg aagcaccacc gttacctgat ccgccttgtt ttgacaagtt
     4681 gaggtagata agcgcgattg caacaccgat aacaccaagt gcgataagtg tcaattggct
     4741 gatagcagca aaggcaaaac caagtgcgaa gaatggccat acttcacgag ttgccatcat
     4801 gttgataacc atagcataac ctacggcaac aaccatacca ccaccaacgg ccataccacc
     4861 tgacaaccaa gctggcattg cttcaagtac tgattgaaca gcttctgcag gaattgcaag
     4921 aagaagagct gcaggaactg cgatacgcaa accttgaagg agaagagcaa acaagtggta
     4981 acgttctaca gcagcgaagt ttgcatcttt agcagcattg tcagcaccgt gtacaagacc
     5041 gactgagata gtacgaacga tcattgtaag gaaaagacca gctacggcaa gagggatagc
     5101 agttgttgtt gcaacggcaa taccttcagt tgagaagtcg ccacctttga tcatgatgat
     5161 tgcagcagct acagaagcaa gagcagcgtc aggagcaaca gctgcaccaa tgtttgccca
     5221 accaagtgca atcatttgta gagaaccacc aagcataaca cctgcagcca agttaccagt
     5281 cacaagaccg ataagggtac aagctaccaa tggttgatgg aattggaatt cgtcaaggat
     5341 accttcaaga ccagcgaaga aggcaacgat gacaacgaga attgcagaaa taagtgtaat
     5401 atctgacatg ttgtaatcct ttctatacta acagatcgat ttattgaacg tttgccttgt
     5461 tgataagttc aaacaagtct ttcttcgaat cgtttggtac tttacgcaca tcaaattcaa
     5521 cgccaaggtc acgcaatttt tcaaaggttg caacgtcatc tttgtccatt gacaatacgt
     5581 tgttaaccat tgttttacca gttgagtgag ccatagaacc aacgttcaat tctttgattt
     5641 caacaccacc ttcaattgct tcaagagctt cttgaggagt ttcgaaaaga atcaaggcgt
     5701 gagtgttacc gaatcgaggg tctttagaaa cctcaatcaa tttcttgatt gggacaacgt
     5761 tcgcttttac gttaccaggt gtagcttgtt tgatgagttg tttacgcaat tcatctttgg
     5821 caactgtatc tgatgcaacg atgatacgat ctgctttgga agcaggagtc caaccagtcg
     5881 ccacttgacc gtgcaagaga cgagtgtcga tacgggctaa gttgattttc aacttgccat
     5941 caccgataac agtacctgga gggatagcac cttgtagtgc aacaggagca gcatcggcaa
     6001 caggagtagc ttcagctggt tgaagttctt ctggaagaac tttgatacca tctttggctt
     6061 ccttgataat gtttgccgca acttcttcca cgccagcagc agcgttaatc atacgctctg
     6121 tgtaagcttg gataagcatt ggcaagttta gaccggtgat gatagcaaac ttacgatctg
     6181 gattttcacc agctacacgg ctcgcttggt tgaatggtga accactccaa aggtcagcca
     6241 aaaccaaaac ttcatcatcg gcatcgaatg cgtccacggc agcatttagc ttagcgtata
     6301 aatcatctgg gccttcactt ggcataaaag taacaacttg tactttttct tgttcgccaa
     6361 agatcattga acctgactga tgaataccag cagcaaattc gccatgactt gcaataatga
     6421 ttccaatact cattattgat cctcctaaat ttttcttgtt catgtaaaac cattgaatgg
     6481 gctttcatgt tcgaactata ccattataaa acgttttcag tatatttgtc aagtaggttt
     6541 gtgttagaaa cgcaaaatat ttctgaaact tttcataatc gtgtttcaga aaagtgataa
     6601 aaatatgcaa cgaatatgac aaaaaactga cagtttatca ggttttttga tgtctataaa
     6661 aataggtttt ctagttcacg tgcgacacca tcttcaaaat tagcaaatgc tgtcaatctg
     6721 tcggcatgcg gacgaagtgt atcgctacaa tttttcatgg cataggctgt tcctgctagg
     6781 tctagcatct caacatcgtt gtgctcatcg ccgaaggcaa ttaagtcgct aggagaggct
     6841 tggtagaggt taagaacata gctcagagcg gttgccttat tgacaccttt tgcacaagtt
     6901 tcgaggatat ttaatggtcc gccccacgta ttgatttcta attcatcttt gaaataggca
     6961 ttcatttctt ttgcaagttc atacttatca gttgcacgag tctgcataag gatggaatga
     7021 ggatcactag tgattaattc aggtttgatg agtgtatctg gagtaatctg ttcaaccccc
     7081 attagagcag ggtcgatctt atctaagttc ttttgagtga tgtagaattt attcttgtat
     7141 tcaccagcaa taaaatctgc ctcgaatgtt tcttcttctt ttagaaattc gagtaggtat
     7201 tttttgtcga ttaaaatatt ttgttcccat tgccatttct ttccagggat gtgaactaaa
     7261 gaaccgttga aattaatcat gggagtatcc agttctagct gttggtaaaa tttttcagcc
     7321 attcgatagg ggcgacctgt ggcgataaga acggtgtgtc ctgcttggcg gaccttcttt
     7381 acagttgaga tggtataatc agataattgg ctatcattat tgagcaatgt gccgtccaaa
     7441 tcgatagcta tgattttttt cttcatattt aatagcattc ctttttagag ttgttatact
     7501 agttttaata gtgaatagtg tttaggtagt agctagattg ccgagaaatt gttttatgcg
     7561 tcaaagaata gtacttgtag gtaaccatat cttgaactaa taatttccag tataacaaaa
     7621 ggagataata aaagcaaaga agcaagtaat tttgtataga ttttttgcaa gtccgcaatg
     7681 ttcgggattt acgaatgttg caagttgaaa atcaataaaa taacgttttc aagattaaaa
     7741 aacttgaaat tagaatgttt tttgattata atgtttttat cgttcgtaaa ttaaaaggag
     7801 aaatgaaaaa atggatcaat tttttaaatt gaaagaacat ggaacgactg tttctacgga
     7861 aattatggca ggacttacga ctttctttgc catgtcatat atcttgtttg ttaaccctag
     7921 tgtgttaagt gttgctggaa tgccaacaca ggctgttttc ttggctacaa taattgccag
     7981 tgctgtatca acgcttgtta tgggattatt tgctaacgta ccttacgcct tggcaccagg
     8041 tatggggttg aatgccttct ttacctatac agtggttatt ggtcttggtt ttacttggca
     8101 agaagctttg gcaatggttt tcctctgcgg tttgtttaac gtttttatca cagttacaaa
     8161 ggttcgtaag agtattatca aggcgattcc agttagttta caacatgcca tcggtggtgg
     8221 tatcggtgta ttcgttgcct atcttggttt taaaaactct aatttaatca ccttcttgac
     8281 atctggttca gatattatta ctgtaaatgg tgttgctccg gcagatgcaa cagcagaaac
     8341 attctcaaac ggtgttttct cagtttttgc tggtggtggt gtcgtacctg gtatttcaac
     8401 ctttactgat ccaagtgttc tcttgacagt gttcggtctc ctcttgacag ctgttttggt
     8461 tattaagaac gtgcgtggag caattttgat tggtattatt gcaacgactg tagctggaat
     8521 tccagcaggt gttgtggatc tgtctacaat cgattttgcg aataacaata tcggtacagc
     8581 ctttgcggag cttggtacaa ctttcctagc ggcttttgat ggtttatctt cgctctttgc
     8641 tgactcaagc cgtctaccac ttgttttgat gactatcttt gcattcagct tgtcagatac
     8701 ctttgacaca cttggtacct ttatcggtac aggtcgtaag actggtattt tctcagaaga
     8761 agatgagaaa gctctcgaaa atggtacagg ctttaactct aagatggaca aggcgctctt
     8821 tgcagatgcg attggtacct ctatcggtgc actatttggt acttcaaata caacgacata
     8881 tgttgaatcg gctgcaggta tctctgcagg tggtcgtact ggtctgacag ctgttacaac
     8941 tgccattctc ttcttactat caattttgat tttaccattc attggaattg tgccaaatgc
     9001 tgcaacagct ccagccttga ttatcgtcgg tgtcatgatg gtttcatcct tccttgatgt
     9061 tgattggagt caattcgaag acgctcttcc agccttcttt gccgccttct tcatggcgct
     9121 ttgcttctct atctcttacg gtattgcagc agccgttatc ttctattgct tggtaaaagt
     9181 ttcaacaggt aaggctaaag aaattcatcc aatcctttgg ggtgccacag ctctatttat
     9241 tctgaatttc atcattctag ccatcttgta atctgtttat tatccttgaa ttgatcttca
     9301 tttgaaggtc agttttttta tttacgatta aaatatatgg gaatcgatgt gatttattca
     9361 gcataatgct gtattgaata ttgtaaattg aaaacggttt tccatttcgt cgcttctctg
     9421 tctcctctta gtgccatttt tactccttgt aactgtattt taaatggctg ttcgttattg
     9481 taagcaagat gtctcatacc tagacatccg ctggattaat gtcatgatgg ggtgaaaaaa
     9541 ttatcatgta gtctttggga gtcacagggc atagaataac ccctgagctt gggatcagga
     9601 ggagttttct aactataatg tttgaatagc gaatgagttg cttgatggat ggtgcgtgct
     9661 gtttcggcat tgttcatagt gtagagatga ataccagcca catcattggt cactaagtca
     9721 acaatttgat ctacagcata ggccagacct gctgcccgaa gagattcagg attgtgctca
     9781 tatttttcta aaatagcttt aaacttacga gggaggtgaa tgttttcaca ggttttcaag
     9841 agtcgaagtg cctgattcct gttgatgata ggcataatac ctgcgataat aggcacttcg
     9901 atgtctgcta gagaacattt ctcctgaaag ttataaaatg cttcgttatc gaagaagagt
     9961 tgagttacca aggttgagca accggcatca actttctttt tcaaattttt gatgtctgag
    10021 acagaatttg gcgattctgg atgtacttct ggataacagg ctccaataat gtcaaaatgt
    10081 ggggcttctt ccttgataaa ggaaatcaag tcagttgcat actggaagtc ctctttaggg
    10141 ggaagagctt caatgatatc gccacgaagt actaaaatct gatgcacacc aatttcatcg
    10201 agagaatgaa gaacggatcg gaccttttct tttgtcaagt aagcagctag caaatgcgcg
    10261 atagttggaa tatgcaattc attgcgaaca tggttagcca acttgacagt agtgtcttct
    10321 acgttcaaat tgttattgct gcatgtaaca ctgataaaat gagggaccaa accttgcatc
    10381 tcatcgagag tttggataat cttttcattg cctacttctg gttttggagg aaaaatttca
    10441 aatgatagac ttggggtttg accaatcata aattagagtt cctttctggc tgtcttagct
    10501 gcttgagtca agtggataag actagctttt gtttctgatt cgccacgagt cttgagtccg
    10561 cagtctgggt taatccaaac tttttctttc ggtactttag caaggatggc ttgaatggtg
    10621 tgtgcaattt catcaacggc agggacccgt ggagagtgga tgtcatagac atcaggacct
    10681 acctgagttt ggaagttttt agccttgagt tcatctagaa taacaagatt tgaacggctg
    10741 gcttcaaagg agataacgtc cgcatccata ttgtcgatgg caggaatgat gtctgtaaat
    10801 tcgccatagc acatatgtgt gtggatttgt gtatctggtg caacagttga gtgtactaaa
    10861 cggaaggcag ggattgccca atcaagatac tcgctgtacc agtcgctacg acggagtggt
    10921 agtttttcac gaagggctgc ttcgtcaatt tggatgatct tgatacccgc tgcttcaagg
    10981 tcaagaactt cttccttgat agcaagggcg atttgaagag ttgattcttt aatagaaata
    11041 tcttcacgag ggaatgacca gttgagaatg gttactggcc ctgtcaacat acctttaact
    11101 ggtttgttgg tacggctttg agcatagcta gaccatttga cagtaattgg attcaagcgt
    11161 gttacatcac cccaaatgat tggtggttta actccacgca taccgtagga ttgtacccaa
    11221 ccgtttttac tgaagaggta gccagacagg ttttcaccga agtattctac catgtcgttt
    11281 cgttcaaact caccgtgaac taagacatcg aagtcaactt cttcttgcca tgcgatccac
    11341 tcatctgttt gagttttgac aaatgcatcg tattcttctt ctgagatatt tcctttacgg
    11401 aaagctaagc gtgtgctacg gacttccttg gtttgaggga aggaaccgat agtggttgtt
    11461 ggaagaagtg gtaagtttag cttctcgcgt tgagtggctt cacgctctgc aaagactggt
    11521 aggcgtgtat agtctgagtc tgtcagtcct gcaagacggt ctgcaagggc tgcatcacga
    11581 ccaacacgtt caagggcaaa gagttccttg ttagctgtga gagcaacttc gccttgaccg
    11641 ttacggatgg catccaaatc acgcaattcg ctcagttttt caactgcaaa ggcaaagtgg
    11701 ttgaggatag ctggttcaaa ttcttcattt gctgttgtga aaggtacatg aagaagggag
    11761 caagaagttg ttatgacgac attttcagct ggaatagtat ccaaaacagc caggctcttt
    11821 tcgtagttgt tgcgccagat atttttaccg ttgacgatac cagcgtagag ggtcttgtca
    11881 gctgggaaac cagttgcaac aagtgtagca gtttctttac cttcttcaaa atcagttaat
    11941 tgtaagagtg tgaatggacc aatcaaaact ggacgagttg tgatgcccaa gttctttgct
    12001 tcttggaatt catcgaaaat cttatgaccg gctagtttga tttctgtgtc ttttt
//
LOCUS       DATUCG010000075        12022 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.076, whole genome shotgun sequence.
ACCESSION   DATUCG010000075 DATUCG010000000
VERSION     DATUCG010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12022
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.076"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            249..890
                     /locus_tag="VCA20_001557"
     CDS             249..890
                     /locus_tag="VCA20_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEP1835360.1"
                     /translation="MIEWIFFDLGSTLLDEEAAYGYYIDKCVKKLESLDIEVSSDSYK
                     KKMVEYAHKSLDPIRATWHYFAPTELRPLWTNEGVSLYPETIDALEKLSQNYRLGIIA
                     NQSSSIRELLKEWGIESYFQLIILSEEVGLSKPDTTIFTLALQKANIPADRVVYVGDR
                     YDNDILPAKSLGMWTVRILTGFGKHASENEKLKSDWVIPSLQEITNIFEQTKN"
     gene            complement(923..1108)
                     /locus_tag="VCA20_001558"
     CDS             complement(923..1108)
                     /locus_tag="VCA20_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835361.1"
                     /translation="MNPVDLFNQVKELIANKDFDGAKQFIEENKDQFGEYLEQAKGLL
                     SGSEGVNGLLDKVKGLF"
     gene            complement(1172..2176)
                     /locus_tag="VCA20_001559"
     CDS             complement(1172..2176)
                     /locus_tag="VCA20_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEP1835362.1"
                     /translation="MTIYFGTYTKRESKGIYKANFDSETGQLSNLELVTEEPNPTYLA
                     FDKAGHLYSVGAENGLGGIAAFNADYTLLNHVVSEGAPLCYVAVDEARDLVYGSNFHK
                     GQLLVYKRLADGSLELADVAQHEGSGPHENQASAHVHFSDLTSDKFLVTCDLGCDQVV
                     AYKVSDQGKVEKIATYQAAPGSGPRHIVFHPVAKIAYLICELNSTIEVLIYDGWGQFE
                     HLQTVSTLPEDHTGFNGTAAIRITKDGKFIYGSNRGNDSIAVYKTLADASLELVEIVP
                     TNGKTPRDFTLSPDEKHLIVVHQDSDNATVFERDVENGCLTELSHDFYVPEAVCVTFL
                     "
     gene            2312..3478
                     /locus_tag="VCA20_001560"
     CDS             2312..3478
                     /locus_tag="VCA20_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEP1835363.1"
                     /translation="MEDKHQKFSLTWFFRLFLNSQAVTFLLVTLLTFLTIFIFSKISF
                     LFRPIGSFLEIVLLPMILTGLLYYLLNPMVDWMEKHKISRTVGISILFVLISLLIIWG
                     LAVAIPSIQEQVTSFAQNLPSNIQKIEGQVTGLLQDQRFEQFRPTALEMLNKVNDQIV
                     AYAQKFSSSAVNWASNLISTASQIIVAILIMPFILFYLLRDGQYLNKHITQYLPTKWR
                     EPIGTVLSDVNGQLSNYVRGQVTVAIIVALMFSVMFSIIGLSYPITLGVMAGFLNLIP
                     YLGSFLAMIPAVILGLIAGPIMLIKVLVVFMIEQTIEGRFVTPLIIGSSLSIHPITIL
                     FVLLTAGQMYGVLGVLLGIPIYASIKVLVKAAFEWYKAHSSLYEDEENEVVNEQ"
     gene            3468..4697
                     /locus_tag="VCA20_001561"
     CDS             3468..4697
                     /locus_tag="VCA20_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEP1835364.1"
                     /translation="MNNSEKMLVCLDQQDLDKADKYFKRALVQDDSETLLSLAAYLEG
                     IGFFPQARQIYEQVKEEFPEVLINLAQIAFEDGLVEESFGYLEEISEDSSVYVEALLV
                     KADLYQAEGLSDVAREKLLEASHLSDDPIILFGLAELDMELELFNEAISYYAQLDNRE
                     IYELTGVSTYQRIGIAYASLGKFEAAIEFLEKAVELEYDDQTVFELAALLFEREEYQK
                     ANLYFKQLDTINPDFEGYEYAYAQSLHAEHKIDDALAMTQKGLAKNDFDANLLLQASQ
                     YAYELHDEAGAEDYLLRAKEVADDSNELALRLSNLYLEQERFEEVVALFDEEVDNVLA
                     RWNMAKAYQALERDDEAIALYDELAGELAENPEFLTDYVAVLRQLGRLDEAKEQASRY
                     IQLVPDDLAMQEFLNEE"
     gene            4701..5418
                     /gene="budA"
                     /locus_tag="VCA20_001562"
                     /pseudo
     CDS             4701..5418
                     /gene="budA"
                     /locus_tag="VCA20_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019322637.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
     gene            complement(5444..7102)
                     /locus_tag="VCA20_001563"
     CDS             complement(5444..7102)
                     /locus_tag="VCA20_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_147463931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HEP1835365.1"
                     /translation="MSFDGFFLHHMTAELKTNLEGGRIQKINQPFEQEIVLNIRSNRQ
                     SHKLLLSAHSVFGRVQLTQSDFTNPKVPNTFTMILRKYLQGAIIEEIRQLDNDRILEF
                     SVSNKDEIGDHIQATLIVEIMGKHSNIILVDKSEQKIIEAIKHVGFSQNSYRTILPGS
                     TYIRPPETHSLNPYTVSDEKLFEILSTQELSPKNLQQVFQGLGRDTASELASHLQTDR
                     LKNFRAFFDHATHPSLTDKSYAALPFANSPENQPHFESLSSLLDFYYQDKAERDRVAQ
                     QANELIKRVASELEKNRKKLVKQEQELADTETAELVRQKGELLTTYLHQVPNDQASVT
                     LDNYYTGEELEIELDVALTPSQNAQRYFKKYQKLKEAVKHLTNLIEETKATIVYLESV
                     DTMLGQASLAEIDEIREELIETGYLKCRHREKIHKRQKPERYLATDGKTIILVGKNNL
                     QNDELTFKMAKKGELWFHAKDIPGSHVVITDNLAPSDEVKTDAAELAAYFSKARHSNL
                     VQVDMIEAKKLHKPTGGKPGFVTYRGQKTLRVTPTEDKIKSMKI"
     gene            7408..8247
                     /locus_tag="VCA20_001564"
     CDS             7408..8247
                     /locus_tag="VCA20_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEP1835366.1"
                     /translation="MIIKRVLNHNAVIAQNNKNVDILLFGKGIAFGKKVGDAIPPSSI
                     EKSFLLKNSDNMNRFTELFIDVPIELVYVCEKIINLGKITLGNHFDEIIYINLTDHIN
                     SSIERHKEGVVVTNPLRWEIAKYYKEEFELGKKALSIIKKDLHVELPVDEAAFIALHF
                     VNANLENIFQESYRITGIIMKIEQIIQDYYSTEFNQDSIDYYRFITHVKLFAHRLVEG
                     NEYNDEDDVDLLELMKKKYPREYQCGTRVADFIRLEYDYLLSPSELVYLIAHIRRLTK
                     NLS"
     gene            8258..10099
                     /locus_tag="VCA20_001565"
     CDS             8258..10099
                     /locus_tag="VCA20_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEP1835367.1"
                     /translation="MKYKNTALAILEAVGGEKNVLRATHCVTRLRLELKDENIVSDER
                     VKSISGVLGIMKKNGQYQIILGNDVANYYKEFTALGKFDSDSVQQVKKANVLEQVIEY
                     IAGSMTPLIPAMLGGGMIKVLVIVLPMLGLLKADSQSISFLAFFGDAPYHFMPIFLVY
                     SASQKLKVTPALAMSVAGILLHPNFVQMVSSGDPLHFLGAPVTPASYGSSVIPILIMV
                     WLMKYIEAVFNKVTPAVTKSFLQPTLVLLVSGFIALVLVGPLGVIVGEGLSQLVEQMH
                     GVAGWLTLAVLGAIMPFIVMTGMHWAFAPIFLAASIATPDVLILPAMLGSNLAQGAAS
                     MAVAFKSKNSNTKQIAFAAGFSALFAGVTEPALYGVTLKYKKPLYAAMIGGGLAGLFV
                     GLTGVKAYLFAVPSLIALPQFIYSEAASNITNAMIAAAISIIVTFILAYFLGIDEETS
                     TVNLEKVAPGISSRKNVFSPLSGQILPLEKVNDATFSKKMLGEGVAIIPKDGKVYAPF
                     DGAVTSLFPTKHAIGLTSDEGVELLIHFGLETVELKGRGFVSHVSDGEKVEKGQLMLE
                     VDVEMLVAEGYDIVTPVVVTNTQEYLDVLLLSTKEEVNYADDLLAVL"
     gene            10114..11538
                     /locus_tag="VCA20_001566"
     CDS             10114..11538
                     /locus_tag="VCA20_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEP1835368.1"
                     /translation="MSIFPDNFLWGGAVAANQVEGAYNEGGKGLSVQDVLPNGGLGEA
                     TQEPTEDNLKLQAIDFYHRYKEDIALFSELGFKVFRTSIAWSRIFPNGDEEFPNEEGL
                     QFYDELFDELLKNGIEPLVTLSHYETPLYLARKYHGWVDRKMIQFFEKFARTVFERYK
                     DKVKYWLTFNEVNSVLELPFTSGGIDLPNEELSKQDLYQAIHHELVASSLVTKIAHEI
                     IPDCKVGCMVLAMPTYPMTPNPEDVWATHAYENLNYLFSDIHVRGYYPNYAKRYFKEN
                     GIKITFVEGDEELLKNNTVDFLSFSYYMSITQAADPSKYSSGKGNILGGLVNPYLEAS
                     DWGWQIDPIGLRLTLNRYYDRYQIPLFIVENGLGAKDQLVKDEAGGLTVHDDYRIQYM
                     QEHLLQVAEAIQDGVEVMGYTSWGCIDCVSMSTAQLSKRYGLIHVDRNDDGSGSLTRY
                     RKKSFYWYKEVIQSNGSILGNSSK"
BASE COUNT         3461 a         2121 c         2656 g         3784 t
ORIGIN      
        1 agtacagtgt actaatttat ccccgagcta gcatagcgag ggttatagta agaacaagtc
       61 cgaagatttt cggacttgtt tttcaatatc tgggagactg ttttatccct gagctagcat
      121 agcgagggat tagtaaaact ctctaggttt tcctagggaa ttttctttgc cttgatgttg
      181 ataaaatgat attctaaaaa ggcagttcaa cttaagtatt ctcattttga aaagaaatgg
      241 agagtgggat gatagagtgg atattttttg atttgggttc gacgctttta gatgaagagg
      301 ctgcttatgg ttattatata gacaagtgtg tgaagaagtt agagtctctg gatatagagg
      361 tttcatcaga ttcttataaa aagaaaatgg tggagtatgc tcataagtcg ctggatccca
      421 ttcgtgcgac ctggcactat tttgcaccga ccgagctacg tcctttatgg acaaatgagg
      481 gtgtgagcct gtatcctgaa acgatagatg ctctagagaa gttatcacaa aactatcgat
      541 tggggattat tgccaatcag tctagtagta ttagagaact gcttaaagag tggggaattg
      601 agtcttattt tcaactcatc atcctctctg aagaggttgg gctatctaaa ccagatacaa
      661 ctattttcac acttgccttg caaaaagcaa acatccctgc agatagagtt gtctatgtcg
      721 gggataggta tgacaatgat attctgcctg ctaaatcttt gggaatgtgg actgtacgga
      781 tactgacagg ttttggaaag catgccagtg aaaatgaaaa attgaagagt gattgggtaa
      841 ttccttcatt gcaagagata acaaatattt ttgaacaaac aaaaaactag actgaacatt
      901 ctcagcctag tttttacatg tattagaaca agcctttaac tttatctaaa agaccgttaa
      961 caccttcaga acctgataac aagccttttg cctgttccaa gtattcaccg aattggtcct
     1021 tgttttcttc gataaattgt tttgcaccat caaagtcttt gtttgcaatc aattctttta
     1081 cttggttaaa aagatctact ggattcatgt tgaaatcacc tcctatgaga ctattaaacc
     1141 aaaactggac ggatttcaca actgaaacga cttataaaaa tgtaacgcaa actgcttcag
     1201 gaacatagaa atcgtgtgaa agctcagtca aacagccatt ttcgacatct ctttcgaata
     1261 cagttgcatt gtcagaatct tgatggacaa caatcaaatg cttttcatca ggactaagcg
     1321 tgaaatcgcg aggagttttt ccatttgttg ggacgatttc aactaattct aagctggcat
     1381 cagcaagcgt tttgtagact gcgatagagt cgtttccgcg gttggatcca taaataaatt
     1441 taccatcctt tgtgatacga atagcggcag taccgttgaa accagtatgg tcttctggga
     1501 gagtagagac agtttgtagg tgttcaaatt gtccccagcc gtcatagata agcacctcaa
     1561 tagttgaatt gagttcacaa atgagatagg cgatttttgc gacgggatgg aagacaatgt
     1621 gacgtggacc acttcctggc gcagcctgat aggttgcgat tttctctact tttccttggt
     1681 cagatacctt ataggctacc acttgatcac atcctaaatc acaggttact aaaaatttat
     1741 cggacgtcaa atcagaaaag tgaacatggg cggaagcttg gttttcgtgc ggtccagatc
     1801 cttcgtgttg agcgacatca gctaattcta gtgaaccatc cgctaaacgt ttataaacaa
     1861 gtagttgccc tttgtggaaa ttggagccat aaactaaatc tcttgcttca tctacagcga
     1921 catagcaaag tggtgcgccc tctgatacaa catggttgag tagagtatag tcagcattaa
     1981 aggcagcgat acctcctaga ccattttcag cgccgacgga gtatagatgt ccggccttat
     2041 caaaggcaag gtaggtaggg ttaggttctt cagtgaccaa ttctagattg ctaagttgtc
     2101 ctgtttcaga gtcaaaattt gctttataaa ttccttttga ttcacgtttt gtataggtac
     2161 cgaaatagat agtcatagga ttctcctttt tggaaatgat tgataaaatt ataccatgtt
     2221 tttctgagaa agtcgtataa attctgtagt tgatttttac aaaaatcctt tctccatgtt
     2281 acaatagaaa aaaagactag gagtgtatta gatggaggat aagcatcaaa aatttagtct
     2341 gacttggttt ttccgcttgt ttttaaatag ccaagcggtg acatttttat tggtaacctt
     2401 gctgactttt ttgaccatat ttatttttag taaaattagt tttttattca gaccaatagg
     2461 aagcttttta gaaattgtat tgttaccgat gattttaaca ggtcttttgt attacctatt
     2521 aaatccgatg gttgactgga tggaaaagca taaaatttct cgtacagttg gtatttccat
     2581 tctctttgtt ttaatcagtc ttctcattat ctggggactg gcggtggcaa ttccgagtat
     2641 tcaagagcaa gtgacatctt ttgcgcaaaa tcttccttcg aatatccaga aaatcgaagg
     2701 acaggtaact gggctcctac aggatcagcg ctttgaacaa tttagaccaa cagctctgga
     2761 gatgttaaat aaggtcaatg atcagatcgt tgcctatgct cagaagtttt cttcaagcgc
     2821 tgtgaactgg gcaagtaatt tgatttcaac cgcttcacag attattgttg ctattttgat
     2881 tatgcccttt attctctttt atctattacg ggatggtcag tatttgaata aacacattac
     2941 ccaatatcta ccaaccaagt ggagagaacc gattggaaca gtactatcag atgtgaatgg
     3001 gcaattgtca aactatgttc gtggtcaggt gaccgttgcg attattgttg ccctgatgtt
     3061 ctcagtgatg ttctcaatca ttggtcttag ctatccaata actttggggg tgatggctgg
     3121 tttcctcaat ctaattccgt atttgggttc tttcttggcc atgattccag ctgttatttt
     3181 aggattgatt gctggtccga tcatgctgat aaaggtttta gtagtcttta tgattgagca
     3241 gactatagaa ggtcgttttg tgacaccatt gattatcgga agctccctaa gtattcatcc
     3301 gattaccatt ttatttgtat tattgacagc tggtcagatg tatggtgtcc taggagttct
     3361 tctgggaatt ccaatatatg cctctattaa ggttttagtg aaagcggcat ttgaatggta
     3421 caaggcacac agtagtttgt atgaagatga agaaaatgag gttgtgaatg aacaatagtg
     3481 aaaaaatgtt ggtttgtttg gaccaacagg atttagacaa ggcagataag tatttcaagc
     3541 gcgccttggt gcaagatgat agtgaaacgc ttttgtcctt ggcagcctac ttagaaggca
     3601 ttggtttttt cccacaggct agacaaatct atgaacaggt caaagaagaa tttcccgaag
     3661 tactgatcaa tcttgcccaa atagcatttg aagatggctt ggttgaagaa tctttcggat
     3721 acttggagga aatttcggaa gacagttctg tctatgtgga agccttgttg gttaaggcgg
     3781 acctgtatca ggcagaaggc ttgtcggatg tggcgcgtga aaaattgtta gaagcaagtc
     3841 acttatcaga tgaccccatc atcctctttg gcttagcaga attggatatg gaactggagc
     3901 tttttaatga ggctatttcc tactatgccc agctggataa tcgtgaaatc tacgaattaa
     3961 cgggagtttc tacctatcag cgaattggta ttgcctatgc aagtttaggg aaatttgaag
     4021 cggccattga atttttggaa aaagctgttg agttggaata cgatgaccag acggtttttg
     4081 agttagcagc tctgctattt gaacgagaag aataccaaaa ggccaatctc tatttcaagc
     4141 agttggatac catcaatcct gattttgagg gctatgaata tgcctatgct cagtcgcttc
     4201 atgccgagca taagattgat gatgccttgg ctatgactca aaaaggcctg gctaaaaatg
     4261 actttgatgc caatctcttg ctccaggctt cacagtatgc ctatgaattg catgatgagg
     4321 cgggggcaga agattatctt ctacgggcaa aagaggtggc agatgatagt aacgaactag
     4381 ctctgcgctt gtccaatctg tatcttgagc aggaacgatt tgaagaggtt gtggctttat
     4441 ttgatgaaga ggtagataac gttctagctc gctggaatat ggccaaagcc tatcaagctc
     4501 tagaaagaga tgatgaagct attgcgcttt acgatgaatt ggctggagaa ttggctgaga
     4561 atcctgaatt tttgactgat tacgtagcgg ttttacgtca acttggtcgt ttggatgagg
     4621 caaaagagca agctagtcgt tatatccaat tggtgccaga cgatttagcc atgcaagaat
     4681 ttttgaatga agagtaggtt atgcaagtaa atcgattatt tcaatacaat accttaggtg
     4741 ccttaatggc tggactgtat ggcggctcct tgacggttgg cgaattgttg gaacatggtg
     4801 atttgggttt gggaaccctg gattccattg acggtgagct gattgtcttg gatggaaagg
     4861 cttatcaggc caagggggca ggggagaaac cagaagtggt tgaagttcca gctgatatga
     4921 aggttcccta tgcggcggtt attttccatg aagcagaagt cattttcaaa caacgctttg
     4981 aaatgactag cgatgaattg caccagcgaa ttgaatccta ttatgatggt gaaaatcttt
     5041 ttcgttccat caaaataaaa gggacgtttt cacggatgca tgtccgcatg attccaaaat
     5101 cggcttctga tgttcgtttt gcggaggtgg caagtccgcc agcctgagta taccgctgag
     5161 aatatttcag gtaccattgt tggtatttgg acaccagaga ttttccatgg tgtcagtgtg
     5221 gctggctatc atttgcattt tatttcggat gatttgacat ttggcgggca tgtcatggac
     5281 tatgttatct cagaaggaat ggtggaagtg gggccagtag atcaactaga ccaacgtttt
     5341 cctgttcaag accgccagta tcttttcgct aagttcaacg ctaaggaagt tagagaagat
     5401 attgataagg cagagtaggt ttcaatacag aaaaatccat gaattagatt ttcatggatt
     5461 ttattttgtc ttcagttggg gtgacacgga gggtcttctg acctcggtag gtcacaaaac
     5521 caggtttgcc acctgtcggt ttgtgaagtt tcttggcttc aatcatatcg acttggacca
     5581 gattggaatg tctggcctta gagaagtagg ctgctaattc agctgcgtct gtcttgacct
     5641 cgtcacttgg ggccaagtta tctgtgatga ccacgtgact gcctggaatg tctttggcat
     5701 ggaaccagag ttcgcctttt ttggccattt taaaggtcaa ctcatcattt tgtaggttat
     5761 ttttcccgac cagaatgatg gttttcccgt ccgtcgccaa gtaacgttca ggtttctgac
     5821 gcttatggat tttctcacga tgacggcatt tgaggtagcc tgtttcaatc aattcctcac
     5881 ggatttcatc gatttctgcc agactagcct gtcccagcat agtgtcaacg gactccagat
     5941 agacaatggt tgccttggtc tcctcaatca agttggtcag gtgcttgacc gcctccttga
     6001 gtttctggta ttttttgaaa taacgttggg cattttggct aggagtaaga gccacgtcca
     6061 actcaatctc tagttcctcg cccgtatagt agttgtctaa ggtcacacta gcctggtcat
     6121 ttggcacctg atgtagatag gtggtcaaga gctcgccctt ttgtcgcacc aactccgccg
     6181 tctccgtatc cgccaattcc tgctcctgct tgaccagttt cttgcgattt ttctccaact
     6241 cactggctac ccgcttgatc agctcattgg cctgctgggc cacccgatcc cgctccgcct
     6301 tgtcctgata gtagaagtcc agcagactgc tcaggctctc aaagtgtggc tggttttcag
     6361 ggctattggc aaatggcaaa gcagcatagg acttgtctgt caggctagga tgtgtagcat
     6421 ggtcaaaaaa ggcacgaaag ttcttcaggc ggtctgtctg caaatggctg gccagttcag
     6481 aagctgtatc ccgccccaaa ccttgaaaga cctgttggag attttttgga ctcagttcct
     6541 gagtcgataa gatttcaaac aatttctcat cagacaccgt ataaggattg agagaatgcg
     6601 tttccggtgg acgaatgtag gttgagcctg gcaaaatagt tcggtaggag ttttgcgaga
     6661 aaccaacgtg cttgatagcc tcaataatct tctgttcaga cttatctacc aagatgatat
     6721 tgctgtgctt gcccatgatt tccacaatca aggtagcttg gatatggtcg ccaatctcgt
     6781 ccttgttgga caccgaaaat tctaagatgc ggtcattgtc caactgacga atctcctcga
     6841 taatggctcc ctgcaagtat ttccgcagaa tcatggtaaa ggtattgggc acttttggat
     6901 tggtaaagtc cgactgagtc agctgaaccc gaccgaaaac cgaatgggcg gacagaagca
     6961 atttatggct ctgacggttg ctgcggatgt taaggacaat ttcctgctca aagggctggt
     7021 tgattttctg aatccgcccc ccttctaaat tggtctttaa ttcagccgtc atgtgatgta
     7081 aaaaaaatcc gtcaaaagac atggttttcc tctttctaat agctataact cagtctagta
     7141 tatcataaat cagtgacttt ttaacgaagg ggactaaaag aagtattctt atatttttat
     7201 aaattattga caaattcttt aatttatatt aaaattgaaa atggttacag agttattaaa
     7261 ttttttaagc tagaagtacc aactaataat tgaaataaaa ggatgttatc gctttaaggc
     7321 gagcaggaac ctatttaaat gaatcgatac gatatatgtc gatttctttt aagtaggttc
     7381 ttttatttta agtttaaagg aaaatttatg atcataaagc gtgtattaaa tcataatgca
     7441 gtcatagcac aaaataataa gaatgttgat atcttgttgt ttggtaaagg aattgcattt
     7501 ggaaagaagg tcggagatgc gatcccacct tcttcgatag aaaaaagttt tttgctcaag
     7561 aatagcgata atatgaatcg ctttacggaa ctatttatcg atgttcctat tgagttggtt
     7621 tatgtttgtg agaaaataat aaatttagga aagataacac ttggaaatca ttttgatgag
     7681 attatttaca tcaatttgac agatcatatc aattctagta tagaaagaca taaggaagga
     7741 gtagtcgtta cgaaccctct tagatgggaa attgccaaat attataaaga ggagtttgaa
     7801 ctggggaaaa aagcattatc catcataaaa aaagatttac atgtcgaact cccagttgat
     7861 gaagcagcct tcatcgcttt gcattttgtc aatgcgaatt tggaaaatat ttttcaagaa
     7921 tcttatcgta ttactggaat tatcatgaag attgaacaaa ttattcaaga ctattattct
     7981 acagaattca atcaagattc tattgattac taccgattca tcacacatgt taaattgttt
     8041 gctcatcgtt tggtagaggg gaatgaatac aatgatgaag atgatgtgga tcttttagag
     8101 ttaatgaaaa agaagtatcc aagagagtac caatgtggaa ctcgagtagc ggactttatt
     8161 cggttagaat atgattatct actgagtcca agtgaattag tctatttaat tgctcacatt
     8221 cggcgtttaa caaaaaattt atcataagga gtctattatg aagtacaaga atactgcatt
     8281 ggcaatccta gaagcagtcg gaggagaaaa gaatgttctt cgtgcaaccc actgcgttac
     8341 tagattgcga ctggaattga aagatgagaa tatcgtgtcc gatgaacgtg ttaaatccat
     8401 ttctggagta ctcggtatta tgaagaaaaa tggacagtat caaattatct taggtaatga
     8461 tgtagctaat tactataaag aatttactgc attaggaaaa tttgattctg attcagttca
     8521 acaggttaaa aaagcaaatg tcttagaaca agtcatcgag tatatcgctg gctctatgac
     8581 accacttatt ccagctatgc ttggtggagg tatgataaaa gttctagtca ttgtcttacc
     8641 gatgctgggg cttttaaaag cggattccca atctatttca tttttagcct tttttggaga
     8701 tgcaccatat cactttatgc cgattttttt ggtctactca gcttcacaaa aactcaaagt
     8761 tacacctgct ttagccatgt cggttgctgg tattttacta catcctaatt ttgttcaaat
     8821 ggtatcttca ggtgatccac ttcatttttt aggtgcccct gttacacctg ctagctatgg
     8881 ttcatcagtt attccaattc ttattatggt ttggctaatg aagtatattg aagctgtttt
     8941 taacaaggtc actccagctg taacgaaaag tttcttacag ccaaccttgg tcctgcttgt
     9001 ttcaggtttc atagctttag ttcttgttgg acctctcggc gttattgttg gtgaaggatt
     9061 atcgcaattg gttgaacaaa tgcatggtgt agctggttgg ttaactttag cagttcttgg
     9121 agcaatcatg ccatttatcg ttatgaccgg tatgcactgg gcttttgcac cgattttctt
     9181 ggcagcttca atcgcgacac ctgatgtatt gattctacca gcgatgttag gttctaactt
     9241 ggcacaaggt gccgcttcta tggcagttgc atttaagagt aaaaatagta acacgaaaca
     9301 aatagccttt gcagctggat tttcagcttt atttgctggt gtcactgagc ctgctttata
     9361 tggtgttact ctaaagtata aaaaaccatt gtatgcagca atgattggtg gtggtctagc
     9421 aggattattt gttggtttga caggtgtaaa agcatacctt tttgcagtac cttctctcat
     9481 tgctttgcca caatttattt actctgaagc agcttcaaac attactaacg ctatgattgc
     9541 tgcggcaatt tcaattattg tcacatttat tttggcctat ttcctaggta tcgatgaaga
     9601 aacttcaaca gttaatttag aaaaagtagc acctggcatt tcaagtagaa aaaatgtctt
     9661 ctctcctctt tcaggtcaaa ttctcccttt agaaaaagta aatgatgcaa cgttttcaaa
     9721 aaaaatgctt ggagaaggag ttgcaattat ccctaaggat ggaaaagtct atgctccatt
     9781 tgacggggct gttaccagtc tttttccaac aaaacatgca atcggtttaa caagtgatga
     9841 aggggtagag ttgcttatcc actttggatt agaaacagtc gagctaaaag gaagaggttt
     9901 tgtaagtcat gtttcagatg gggaaaaagt tgaaaagggt caactgatgc tggaagtgga
     9961 tgtagaaatg cttgtcgcag aagggtacga tatagtgact cctgtagttg ttacaaatac
    10021 acaggaatat ctggatgttc tcttgctttc gacaaaagag gaagtcaact atgctgatga
    10081 tttattagca gtcttataat tcgaggaggg tatatgtcaa tttttccaga caatttctta
    10141 tggggaggag cagtagctgc aaaccaagta gaaggtgcat ataatgaggg aggaaaagga
    10201 ttatccgttc aagatgttct ccccaatggt ggactgggtg aagctaccca agaaccaacg
    10261 gaagataatt tgaaattaca agctattgat ttttaccatc gatacaagga agatatagcc
    10321 ctgttttcag agttgggatt taaggtattt agaacatcaa ttgcttggag tagaatattt
    10381 ccgaatggag atgaagaatt tccaaatgaa gaagggctac agttttatga tgaacttttt
    10441 gatgaacttc taaagaatgg tatagagcct cttgtaactc tctctcacta tgaaactccc
    10501 ctgtatctgg caaggaaata tcatggctgg gtggatagaa aaatgattca attttttgaa
    10561 aaatttgctc gtacagtttt tgagcgatac aaagataagg ttaagtactg gttgacattt
    10621 aacgaggtca attctgtctt agaactgcct tttacaagtg gtggaattga cttgccgaat
    10681 gaagaacttt caaaacaaga tttatatcag gccattcacc atgaacttgt agcttctagt
    10741 cttgttacca aaattgctca tgaaattatt cctgactgta aggttggctg tatggtctta
    10801 gctatgccga cctatccaat gacacccaat cctgaagatg tttgggcgac tcatgcctat
    10861 gaaaatttga actatttatt ttcggatata catgttagag ggtattatcc taattatgcc
    10921 aaaagatact ttaaagaaaa tggcataaag attactttcg tggaaggtga tgaagagtta
    10981 ttaaaaaata atacggttga ttttctctcc ttcagttatt atatgagtat tactcaggca
    11041 gcagatccat caaaatattc atctggaaaa ggaaatatct tgggtggttt ggtcaatcct
    11101 tatttagaag cttctgattg gggttggcag attgatccaa ttggtttaag attgacacta
    11161 aatcgttatt atgatcgata ccagattcct ttattcattg ttgaaaatgg tttgggagct
    11221 aaagatcagc tagtcaagga tgaggctggt ggtttgactg ttcatgacga ttatcgaatt
    11281 caatacatgc aagaacacct tttacaagtt gcggaggcga tacaggatgg cgtagaagtc
    11341 atgggttata cttcttgggg ctgtattgat tgtgtatcta tgtctaccgc acaattatct
    11401 aaaagatatg gactaattca tgttgataga aatgatgatg gaagcggtag tctaactaga
    11461 tatagaaaga aatcatttta ctggtataaa gaagttattc aatccaatgg cagtatcctg
    11521 ggcaatagct caaaatagac tcttaaggta ttaaaaagtt agtaaaaagt tttcagaaaa
    11581 tttaatcaaa agtattgaca aagtgatttt gataccgtat aatcatctgt aatcagaaga
    11641 agtagtaaag cagaagtctt cagggagcct gtggtgctga gaacgggtgg cggagtttta
    11701 tgaaatgggc tgatgtgcat ttatgtctat tttatgacgt cgttagtcgg tcttgccgta
    11761 ccaccagtac tgccttcgcc cgactgccta gtcctaaaat gccctaaatt cacaaggatt
    11821 gtttggcata acttactttc agtaatcagc caaaacagtc acgcaaatga acttaaaaca
    11881 atgatagttc ggtcggcaca ccttacagtg caactccttg ttagaggaga tgagatatgg
    11941 gaggattcta gacaattccc ataagtgagg tggcaccgtg tcattgacgc cctcgcagga
    12001 agttttttcc tgcgggggct tt
//
LOCUS       DATUCG010000076        11968 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.077, whole genome shotgun sequence.
ACCESSION   DATUCG010000076 DATUCG010000000
VERSION     DATUCG010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11968
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.077"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            <1..574
                     /gene="uxuA"
                     /locus_tag="VCA20_001567"
     CDS             <1..574
                     /gene="uxuA"
                     /locus_tag="VCA20_001567"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010781258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEP1835369.1"
                     /translation="LEEMYAGITEEKLFENLSYFLKEIIPVCEEANVKMAIHPDDPPF
                     DMFGYPRITKNKEDILKIVNIVDSPYNGVTLCTGSLGANPDNDVVDIIHSLRGKINFA
                     HLRNVKILGDKHFLESAHLSSEGSLDMYAIVEALIETGFDGIARPDHGRTIWGEVARP
                     GYGLYDRAMGLTYLQGLEEAAIKAHNFKVK"
     gene            582..1250
                     /locus_tag="VCA20_001568"
     CDS             582..1250
                     /locus_tag="VCA20_001568"
                     /inference="COORDINATES: protein motif:HMM:NF024811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEP1835370.1"
                     /translation="MGYLDLKKIDNVIFDFDGLLVNSEDVIGECWIKTCEYYGIHYPE
                     GFFDDIVGVSKDVTYNLLIDYMDVVIGEKEFFERRALFIEEEVNKGNIHLLPGVQEIP
                     DLLKTIEIPMYIATSSSRFWPINITERLGILTYFKGIYGYEDVEKVKPSPELYLKVVS
                     EHQLNKSTTVIFEDSLSGIQAATSAGIDTIVVNAKKYMFSSEMNQKILFQIKGFDELK
                     TDLE"
     gene            1289..2158
                     /locus_tag="VCA20_001569"
     CDS             1289..2158
                     /locus_tag="VCA20_001569"
                     /inference="COORDINATES: protein
                     motif:HMM:NF013539.1,HMM:NF013577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEP1835371.1"
                     /translation="MQNIFLKKTIKDSRNKLSELEIQLSEYLLGLRGEELINKTISDM
                     AEESDISQSTIYNFVKKIGFSGFKDFKLQLAQNISFDSGSNSSRIITNSSITREDLLM
                     DIAKKVISFNQSAFDSLLYFLDEETLSNIMLDIDNAKSLNFFGQGGSSVVAFDAYQKF
                     MRTKYWCNYDIDYHIQLEKCVKLSKTDVCFLFSHSGETKETITIARLLKIRGCKLISI
                     TGNPNSELINLCDHSIIVYTNEAKFRTESLTSRILYLTIIDILYTCVMIRNEEENSLT
                     LKKIRQIMKDVKN"
     gene            2349..3047
                     /gene="dapD"
                     /locus_tag="VCA20_001570"
     CDS             2349..3047
                     /gene="dapD"
                     /locus_tag="VCA20_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HEP1835372.1"
                     /translation="MTAQKMTAQEIIAFIGNAVKKTTVKVTFEGELAGPVPAEVTKLG
                     NVLFGDWKDVEPLLTNLTENVDYVVEQDGRNSAVPLLDKRNINARIEPGAIIRDQVTI
                     GDNAVIMMGAVINIGAEIGPGTMIDMGAILGGRATVGKNSHIGAGAVLAGVIEPASAE
                     PVRVGDNVLVGANAVVIEGVQIGSGSVVAAGAIVTQDVPENVVVAGVPARIIKEIDAQ
                     TQQKTALEEALRTL"
     gene            3057..4184
                     /locus_tag="VCA20_001571"
     CDS             3057..4184
                     /locus_tag="VCA20_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HEP1835373.1"
                     /translation="MLDLIATRRALHQIPELGMEEFKTHAFLMETIENLLEDCTFAQV
                     RTWKTGILVYLTGSAPEKTIGWRADIDGLPIVEETGLDFKSLHPDRMHACGHDFHMTI
                     ALGLLEKMAEQQPKNNLLFLFQPAEENLAGGMLMYEAGAFGDWLPDEFYGLHVRPDLK
                     VGQMATNRATLFAGTCEVKIRFTGKGGHAAFPHTANDALVAASYFVTQVQSVVSRNVD
                     PIEGAVVTFGAMHAGTTNNVIAETAFLHGTIRALTQNMSLLVQKRVREVAEGIALSCG
                     VDLDIELNPSGYLPVENNPQLADELMTYFDGIDGVEMIDCPPAMTGEDFGYLLNKVPG
                     VMFWLGVDTPYPLHNPHLSPKEEVLPFAVDKLSYFLKMKAR"
     gene            4225..4767
                     /locus_tag="VCA20_001572"
     CDS             4225..4767
                     /locus_tag="VCA20_001572"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEP1835374.1"
                     /translation="MDKVSIRKEVLQNLKGLPSSQRARWSQQLTERLLASDTYQGAKS
                     LGTYLSMPHEFDTSYLIEQAQKDGKCIFIPKTYSQGRMDFVEYNPDDLVKSRFGVWEP
                     GTYSQPVDKSVVKLIHVPGLAWNQAGFRVGYGGGFYDRYLADYKGETVSTLADFQVYD
                     FEPDVFDIPVKELLIFEELF"
     gene            4751..5428
                     /locus_tag="VCA20_001573"
     CDS             4751..5428
                     /locus_tag="VCA20_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEP1835375.1"
                     /translation="MKNFFDKRYPVTNGLLAVTALVFLLIQVFRFGQTTTAYTIFEFG
                     GMYGQVVRYDPTQLWRLISPIFVHIGWEHFIFNSITLLGLGYQLEELFGSRRFFLLYL
                     LSGIMGNLFVLFFTPDVVGAGASTSLFGLFAAMALLRKFSRSPYLQVLGQRYMVLLGL
                     NLVLGLFNPTISMAGHIGGAVGGCLVVMFLPPLVEKDLFTGKQIFYSFIGYLGLIALL
                     LGIFYVT"
     gene            complement(5446..6348)
                     /gene="galU"
                     /locus_tag="VCA20_001574"
     CDS             complement(5446..6348)
                     /gene="galU"
                     /locus_tag="VCA20_001574"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEP1835376.1"
                     /translation="MTKKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVE
                     EALRSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKNDLLKLVDETTGIRLHFI
                     RQSHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITDTKAVPLTKQLMNDYEKTHAST
                     IAVMPVPHEEVSAYGVIAPQGEGVNGLYSVETFVEKPKPEDAPSDLAIIGRYLLTPEI
                     FEILENQKPGAGNEIQLTDAIDTLNKTQRVFAREFKGNRYDVGDKFGFMKTSIDYALQ
                     HPQVKDDLKRYLIDLGKKLDTKKN"
     gene            complement(6364..7380)
                     /locus_tag="VCA20_001575"
     CDS             complement(6364..7380)
                     /locus_tag="VCA20_001575"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEP1835377.1"
                     /translation="MTKQTIAILGPGSWGTALGQVLNDNGHTVRIWGNVPEQIEEINK
                     EHTNKRYFKDVILDENIKGYKDLSEALDGVDAVLFVVPTKVTRLVAKQVAQALKHKVV
                     VMHASKGLEPDSHKRLSEVLEEEIPAELRSEIVVVSGPSHAEETIIRDLTLISAASKD
                     LETASYVQNLFSNHYFRLYTNNDVIGVETAGALKNIIAVGAGALHGLGYGDNAKAAII
                     ARGLTEITRLGVAMGANPLTYSGLSGVGDLIVTGTSVHSRNWRAGDQLGRGEKLEDVE
                     RNMGMVIEGISTTKAAYELAQELGVYMPITQAIYKVIYQGAGIEDAIKEIMTGEFRHE
                     NEWS"
     gene            7602..8051
                     /locus_tag="VCA20_001576"
     CDS             7602..8051
                     /locus_tag="VCA20_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEP1835378.1"
                     /translation="MGHTIADFRNLLNQIEQISETIAKEYDVEHLAGPQGWALRFIAE
                     RSDLETFVKDIEAELKISKSVASNLVKRMEKNDFIQVLPSQFDRRYKQLVLTEKGRSK
                     ICHLKSFHEEMHHSLFRGIQKEEFDLIRQVADQLKENIKRYKETNHV"
     gene            8044..9753
                     /locus_tag="VCA20_001577"
     CDS             8044..9753
                     /locus_tag="VCA20_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003105695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1835379.1"
                     /translation="MFKEAILRYKWYALASVLLTSIVVATALMQPSYLQDVLTAVLAN
                     DKEEIVRVGKLLLIIAGIGLLAGLVNTIAAAKISQGVSADIREKTFRKIQSFSYANIE
                     EFNAGNLVVRMTNDVNQIQNLVMMLFTVLMRVPLLFVGAFIMAVRTMPELWWMIILMV
                     VLIMAIMAVVMGQMGPRFGKFQSLMDKMNSIAKENLRGIRVVKSFVQEKNQYAKFKEV
                     SNELLDLNLFIGYGFAILQPLMMFISYMAIFASLYLVSGMLETNLEAVGGFTSFMSYL
                     MQIMFAIIMASFIGMQASRAAISIKRISEVLDTEPAMTFKDVADEELTGKIVFDNVSF
                     TYPHDTEPTLKNISFEIEPGQMVGVVGATGAGKSTLAQLIPRLFDPQEGTVSIGGRDL
                     KEVSQNTLRDTVSIVLQKAILFSGTIADNLRQGAPGADLQRLERAAGIAQAKEFIDRL
                     DDTYESQVEERGNNFSGGQKQRMSIARGVIGEPKILVLDDSTSALDAKSEKLVQEALN
                     HDLKETTTVIVAQKISSVVKADKILVLDEGRLIGQGTHAELVATNDVYREIYETQKGR
                     EEN"
     gene            9893..11677
                     /locus_tag="VCA20_001578"
     CDS             9893..11677
                     /locus_tag="VCA20_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1835380.1"
                     /translation="MKTLRFFWFYFKRYKLSFAVIFLAIVAATYLQVKTPVFLGNAIA
                     EMGKIGQAYFMANQAGQTDFQPDMADFNGVMLNLFFAYAATVVASLIYTLLFTRIVAH
                     STNRMRKGLFGKLERLTVAFFDSHKDGDILSCFTSDLDNIQNAFNQSLTQVVTNIALY
                     VGMVIMMFRQDTRLALVTIASTPVALIALVVIIRLSRKYTDKQQAAVSKLNAYMDEKI
                     SGQKAIIVQGVQEETIDGFLELNEEVRRTTFKGRLFGGILFPFMNGMSLVNTAIVIFA
                     GSSIVLNDSSLETAAALGLVVTFVQYSQQYYQPIMQVAASWAELQLAFTGAHRIQEMF
                     DEPEEVRPQNAPLFTELKEGVEIKDIDFGYLPGQKVLDKVSISAPKGKMVAVVGPTGS
                     GKTTIMNLINRFYDVNGGSVAFDGRDIREYDLDSLRDKVGIVLQESVLFSGTIADNIR
                     FGDESISQEMVETAARATHIHDFIMSLPEGYETFVTDDENVFSTGQKQLISIARTLLT
                     DPQVLILDEATSNVDTVTEAKIQKAMEAIIAGRTSFVIAHRLKTILNADEIIVLKDGK
                     VIEQGNHSQLLKQGGFYAELYHNQFVFE"
BASE COUNT         3310 a         2282 c         2791 g         3585 t
ORIGIN      
        1 attagaagaa atgtatgctg ggattactga agaaaaatta tttgaaaatt tatcatattt
       61 tctgaaggag attattccag tttgtgaaga agcaaatgta aaaatggcaa tccatccaga
      121 cgatccgccg tttgatatgt tcggatatcc aagaattaca aagaataagg aagatatttt
      181 aaaaatagtt aatattgttg attctcctta taatggtgta accttgtgta ctggctcatt
      241 gggagctaat cctgataatg atgttgtgga cattatccat tcattgagag ggaaaattaa
      301 ttttgctcat cttagaaatg taaaaatttt aggcgataag cattttttag aatctgcgca
      361 tctaagctca gaagggtctt tggacatgta tgcgattgtc gaagcattaa ttgagaccgg
      421 atttgatggc attgctagac ccgatcatgg aagaacaatt tggggcgagg ttgctagacc
      481 tggctatggc ttatatgatc gggcaatggg cttgacctat ttacaaggat tggaagaagc
      541 cgctattaaa gcacataatt ttaaagttaa gtaggtatat tatgggctac ttagatttaa
      601 agaagattga taatgtcatt tttgattttg atggtttact cgttaattct gaggatgtaa
      661 ttggtgaatg ttggataaag acatgtgagt attacgggat tcattatccg gaaggatttt
      721 tcgatgatat tgtaggtgta tcaaaagatg tcacgtataa tttgttgatt gattatatgg
      781 atgttgtaat cggagagaaa gaattttttg agcgacgtgc attatttatt gaggaagaag
      841 tcaacaaagg taatatacac ttattaccgg gggttcaaga aattccggat ttattgaaaa
      901 caatagaaat accaatgtac atcgccactt cttcaagtag attttggccg attaatatta
      961 cagaaaggtt aggtatatta acttatttta aaggtatcta tgggtacgag gatgttgaaa
     1021 aagtcaagcc gagtccagag ttatatctca aagtagttag tgaacatcag ttaaataaaa
     1081 gtacgacagt catttttgaa gattcacttt ctggcataca agcagcaaca agtgcgggta
     1141 ttgatacaat tgttgtaaat gccaaaaagt acatgttttc aagtgaaatg aatcaaaaga
     1201 tcctctttca aataaagggt tttgatgaat taaaaacgga tcttgaatag gtccgttttg
     1261 ttgctaatat ggaagggaga taagtagcat gcaaaatatt tttttaaaaa aaacaataaa
     1321 agattcgcgt aataaattga gtgagttaga aattcaatta tcagagtatt tattggggct
     1381 gagaggagaa gaactaatta ataaaacaat ctcagatatg gctgaggaga gcgatatttc
     1441 tcaatcaaca atatataatt ttgtgaaaaa aattggtttt tctggattta aagattttaa
     1501 attacaattg gctcaaaata tttcttttga ttctggctct aattcgagta gaatcataac
     1561 aaattcttca ataacaagag aagatttact tatggatatt gctaaaaaag tgataagttt
     1621 taaccaatca gcttttgata gtttgctata ttttttagat gaagaaactc tgagcaatat
     1681 tatgttggat attgacaatg ctaaaagtct caattttttt ggacaaggcg ggtcatcagt
     1741 tgttgcattt gatgcatatc aaaaatttat gagaacaaag tactggtgca actatgatat
     1801 tgattaccat attcagttag agaaatgtgt aaaattaagc aaaacagacg tttgtttttt
     1861 attttcccat tctggggaaa ccaaagaaac gattacaatt gctcgacttt taaaaatacg
     1921 tggctgtaag ctgatttcta ttactggaaa tccaaatagt gaattgatta atttatgtga
     1981 ccattcgatt atcgtataca caaatgaagc taaatttagg acagagagcc taacatcgag
     2041 aatattatac ctaactatta ttgatattct atacacttgc gtaatgattc gaaatgaaga
     2101 agaaaatagt cttactttaa aaaagattag acaaattatg aaggatgtta aaaattaaag
     2161 taaatatcca attagttagt agtataaatt ggagttaaga tttcaatgat tgagaaaaat
     2221 gcagattcat attagacctt ctagataaaa acttcgaagg tctttttttc atttccaaac
     2281 catattcccc gaattttctg aaaatttttg gtataatagt aaaaaataat ctttagatag
     2341 gaaaatatat gactgcacaa aaaatgactg cgcaagaaat cattgctttt atcggtaatg
     2401 ctgtgaagaa gacaactgtt aaagtgactt ttgaagggga attagctggt cctgttcctg
     2461 cagaagtgac aaaacttggc aacgtcctct tcggtgactg gaaagacgtc gagccacttt
     2521 tgacaaactt gactgaaaat gtagactacg tggttgagca agacggccgc aattcggctg
     2581 tgcccttgtt ggacaaacgg aacatcaatg ctcgtattga accaggtgcg attattcgcg
     2641 accaagtgac gattggcgat aatgccgtta tcatgatggg cgcggtgatt aacatcggtg
     2701 cggaaatcgg cccaggtacc atgattgata tgggtgcgat tttgggcggt cgtgcgaccg
     2761 ttggtaaaaa cagccacatc ggtgcaggtg cggtccttgc aggtgttatc gagccagctt
     2821 ctgccgagcc agttcgtgtc ggcgacaatg tcttggtcgg tgccaatgct gttgtcatcg
     2881 agggtgtgca aattggtagc ggctctgttg tggcagctgg tgcgatagtg acccaagatg
     2941 tccctgaaaa cgtagtggtg gcaggagtac cagctcgcat catcaaggaa atcgacgctc
     3001 aaacccaaca aaaaacagcc ttggaagagg ctttgcggac actctaagag gtagctatgc
     3061 tagatttaat cgcgacacgc cgggccctcc accagattcc agagctgggc atggaggagt
     3121 tcaagacaca cgcctttctt atggaaacta ttgagaactt gctggaagat tgtacttttg
     3181 cccaagtccg cacttggaag acaggcattt tggtctatct gacgggctct gctccagaaa
     3241 aaacgattgg ctggcgggcg gatattgacg gtcttccgat cgtggaggag acgggcctgg
     3301 acttcaagtc cctccatcca gaccgtatgc acgcctgtgg acatgatttc cacatgacca
     3361 ttgccttggg gcttttggag aaaatggcag agcagcaacc gaaaaacaat cttctcttct
     3421 tgtttcagcc tgcggaagaa aatctagcag gtggcatgct catgtatgaa gcgggagctt
     3481 ttggagattg gttgccagat gaattttatg gactacatgt tcgaccagac ctcaaagtcg
     3541 gtcaaatggc caccaaccgt gcgaccctct ttgctgggac ctgtgaagtc aagatacggt
     3601 ttacaggaaa gggtgggcac gcagcatttc cccataccgc aaatgacgcc ttggtggcag
     3661 ccagctactt tgtgacccag gtccagtcgg tggttagtcg aaatgttgac ccaatcgagg
     3721 gagctgtggt gaccttcgga gccatgcatg cgggtacgac caacaatgtc attgcggaaa
     3781 cggccttctt gcacggcacc attcgggcct tgacccagaa catgagtctt ctggtgcaaa
     3841 agcgggtgcg ggaagtggca gagggaatcg ccctgtcctg tggagtagac ttggacatcg
     3901 agcttaatcc tagcggttat ctgcctgtgg aaaataatcc tcagctggcg gatgaactca
     3961 tgacctactt tgatggcatt gacggagtgg agatgattga ctgtccacct gccatgacgg
     4021 gtgaggactt cggctacctg ctcaacaagg ttccaggtgt tatgttctgg ctgggtgtgg
     4081 atacgcctta tccccttcac aatccacatc tcagtcccaa ggaggaagtt cttccctttg
     4141 ctgtggataa gttgagttat tttttgaaaa tgaaagcaag atagactggt ttttccagtc
     4201 tttttctgaa agaaaggagt ttctgtggat aaagtgagta tacggaaaga agttctgcag
     4261 aacttgaaag gcctgcctag tagtcagcga gcaagatgga gtcagcaact aacggaacgc
     4321 ctccttgctt ctgatactta tcagggcgca aagtcgcttg ggacctacct ttccatgccc
     4381 catgaatttg atacttctta tctgattgaa caggctcaaa aggatggaaa atgcattttc
     4441 attcctaaga cctattcaca agggcgtatg gattttgtag aatataatcc tgatgatttg
     4501 gtgaaatcca gatttggagt ttgggaacct gggacttatt cacaacctgt ggataagtct
     4561 gtggttaagt tgatccatgt ccctggttta gcttggaatc aagcgggttt tcgagtgggc
     4621 tatggaggtg gtttttatga tcgctaccta gcagattata agggggagac ggtatcgacc
     4681 ttggcagact ttcaagtcta tgatttcgaa ccagatgtat ttgatattcc agtaaaggag
     4741 ttgttgattt ttgaagaact tttttgataa gcgctaccct gtgaccaatg gcttgttggc
     4801 agtcacggct cttgtttttc ttttgataca agtatttcga tttgggcaga ccactacagc
     4861 ctatacgatt tttgaattcg gtggtatgta tggtcaagtg gtgcgttatg atccgactca
     4921 gttgtggcgc ttgatttccc ctatctttgt ccacattggt tgggaacact ttatctttaa
     4981 cagcattacc ttgcttggtt tgggttatca attagaagaa ctgtttggct caaggcgctt
     5041 ctttctcctc tatctcttgt ctgggataat gggcaatctt tttgttctct tttttacacc
     5101 agatgtagtt ggtgcaggtg catcgacctc gctctttggt ctttttgcgg ctatggcact
     5161 tttgaggaaa tttagtcgca gcccttatct acaggttctt ggtcaacgct acatggttct
     5221 tttgggattg aacttggtct tgggactttt taatccaacc attagtatgg caggacatat
     5281 tggaggagct gttggaggtt gcttggtcgt gatgttcctt ccacctcttg tggaaaaaga
     5341 tctctttaca ggaaaacaga tcttctacag tttcattggt tatctgggtc tgatcgccct
     5401 attattaggg atattttatg tgacataaga aaaaactagc gcttgctagt ttttctttgt
     5461 atctaatttt ttaccaagat cgatcaagta tcgtttcaaa tcatctttta cttgagggtg
     5521 ttggagggcg tagtcgatag aagttttcat gaaaccgaac ttatccccaa catcgtaacg
     5581 gttgcctttg aattcacgag caaacacgcg ttgtgtttta ttgagtgtat cgatagcatc
     5641 tgtcaattga atctcgttcc cggcacctgg tttttggttt tctaaaatct caaagatttc
     5701 aggagtgagt aagtagcgtc cgataatagc aaggtcagaa ggagcatctt ctggttttgg
     5761 tttttccaca aaggtttcca cactatagag accgttgaca ccttcacctt gtggagcgat
     5821 aacaccgtat gcagaaactt cttcatgagg aacgggcata acagcaatgg ttgaagcatg
     5881 tgttttttca tagtcgttca tgagttgctt ggtcaatggg acagcttttg tatctgtgat
     5941 gtccatgagg tcatcgccca gcatgacaac aaatggttcg ttcccgacaa atgctttggc
     6001 ttgaagaacg gcatcaccaa gtccacgtgg atggctttga cgaatgaagt gtagtcgaat
     6061 gcctgttgtc tcatcaacaa gtttcaacaa atcatttttg cctttttctt tgaggttgta
     6121 ttccaattca aagtttgaat caaagtggtc ttcgatggaa cgctttgatt taccagtgac
     6181 caccaaaata tcttcgatac cagagcgaag agcttcttca acgataaatt gaatagttgg
     6241 tttatcaaca atcggcaaca tttctttggc aagagcctta gtagcaggca agaaacgtgt
     6301 tcccaaacct gctgcaggga taacggcttt tctaactttt tttgtcattg tatctccttt
     6361 ttattaagac cattcattct catgtcggaa ttcccccgtc atgatttctt tgatggcatc
     6421 ttcaataccc gcaccttggt agatgacctt gtagatggct tgagtaatag gcatgtaaac
     6481 gcccaattcc tgtgccaact cataggctgc tttagttgtt gaaatacctt caatcaccat
     6541 tcccatattg cgttctacat cttcgagttt ttcaccgcga ccgagctgat ctccagctct
     6601 ccagttgcgg gagtggacag atgtaccggt aacaatcaaa tcaccaacac cagaaagacc
     6661 gctgtaagtc agaggatttg cgcccatagc aacgcctaga cgtgtgattt ctgttaaacc
     6721 gcgagcgata atagctgcct tggcattatc accataacca agtccgtgta aagcgccggc
     6781 accgacagca ataatatttt taagcgcacc tgctgtttct acaccgatga catcattatt
     6841 tgtgtaaagt cggaagtagt ggttgctgaa aagattttgg acgtagctgg ctgtttccaa
     6901 gtcttttgaa gctgcagaaa tcaaggtcaa atcacggatg atagtctctt ctgcatggct
     6961 aggacctgaa accacgacga tttcagaacg gagttcagca ggaatctctt cttccagtac
     7021 ctcagacaag cgcttgtgac tatcaggctc taagcctttt gaagcgtgca tgacaactac
     7081 cttgtgtttg agagcttgtg cgacttgctt ggcaactagg cgtgttacct tggttggtac
     7141 aacaaaaaga accgcatcaa caccgtccaa ggcttcactc aaatccttat atcctttgat
     7201 gttttcatct aggataacat ccttgaaata gcgcttattt gtatgttctt tattgatttc
     7261 ctcgatttgt tcggggacat ttccccaaat acggacagta tgaccgttgt cgttgagaac
     7321 ttgtcctagg gcggtgcccc atgaaccggg tcccaagatg gcaatggttt gtttggtcat
     7381 aaaaaatcct ttctagttgg tgtaatttag atatattata ccataattga tacataaaat
     7441 cttatataat ttatacggta tatatattat gggtttgaag tgcgcaatga tgctgaaata
     7501 acaaaatatt ttcagaaaag agcaaacgaa ataattgaca atagttctta agagaactat
     7561 aatagttctc atggtaatta ttttcgtaga aaggagctgt catgggacat actattgcag
     7621 attttcggaa tttactcaat caaattgaac aaattagtga aacgattgcc aaggaatatg
     7681 atgtggagca cttggctggt ccacagggct gggccttgcg tttcatagcg gaacggtcag
     7741 accttgaaac ctttgtcaaa gatattgaag cggagttgaa aatttccaaa tccgttgcca
     7801 gcaatctggt taagcgaatg gagaaaaatg actttattca ggttttgcca tctcagtttg
     7861 atagacgtta caagcagttg gtcttgacag agaaaggtcg gagcaagatt tgtcacctaa
     7921 aatccttcca tgaggagatg caccattctc tttttagagg tattcaaaag gaagaatttg
     7981 atctgatcag acaggtagcc gatcaattaa aagaaaatat taaacgttat aaggagacga
     8041 atcatgttta aagaagccat tttacgttat aaatggtacg ccttagcatc ggtcttgctg
     8101 acgtcaattg tcgtagcaac ggccttgatg caacctagtt atttacagga tgttttgaca
     8161 gcagtcttgg ccaatgacaa ggaggaaatt gttcgtgtgg gcaaattact gctgattatt
     8221 gcaggaattg gtcttttggc agggcttgtc aataccattg ctgcagcaaa aatttcacaa
     8281 ggggtatctg cagatattcg tgagaagacc tttcgtaaga ttcaaagctt ctcttatgcc
     8341 aatatcgaag agtttaacgc agggaacttg gttgttcgga tgactaacga tgtcaatcaa
     8401 atccagaacc ttgtcatgat gctcttcaca gtgttgatgc gggttccatt gctgtttgtc
     8461 ggagccttca tcatggcggt gcggaccatg ccagaactct ggtggatgat tattctcatg
     8521 gtggtcttga ttatggccat tatggcagtt gtcatggggc aaatgggacc acgttttggg
     8581 aaattccagt ccctcatgga caagatgaac agcattgcca aggaaaatct ccgtgggatt
     8641 cgtgtggtta aatcctttgt tcaggagaaa aatcaatatg caaaattcaa ggaagtatct
     8701 aatgaattgc tagatttgaa cctctttatc ggctatggtt ttgctatctt gcagcccttg
     8761 atgatgttta tctcttacat ggcgattttt gcatcgcttt acttggtatc tgggatgttg
     8821 gaaaccaact tggaggcagt tggtggcttt acttccttta tgagctatct catgcaaatc
     8881 atgttcgcta tcatcatggc cagcttcatt ggtatgcagg catctcgtgc ggcgatttct
     8941 attaagcgga tcagcgaggt gttggataca gagcctgcta tgaccttcaa ggatgtggcg
     9001 gatgaagaat tgactggtaa gattgtcttt gacaatgtta gcttcaccta tccacatgat
     9061 acagagccga cgttgaaaaa tatctcattt gagattgagc cagggcaaat ggtcggtgtg
     9121 gttggtgcaa caggtgcggg taaatccact cttgctcaac tgattccgcg tttgtttgac
     9181 ccgcaagaag gaacggtatc catcggtggt cgggacttga aagaagtcag ccagaacacc
     9241 ctgcgtgata cggtatcgat tgtcttgcaa aaggctattc tcttctcggg aactattgca
     9301 gacaacctgc gtcaaggggc acctggtgct gatttgcaaa gattggagcg ggccgcaggt
     9361 attgcccaag caaaagaatt tatcgaccgc ttggatgata cctatgagag ccaagtagaa
     9421 gaacgtggaa ataacttctc tggtggtcag aagcaacgga tgtccattgc tcgtggggtg
     9481 attggtgagc ctaagatttt ggtcttggat gactcaactt ctgccctgga tgccaaatct
     9541 gagaaactag tccaagaggc tctcaaccat gatttgaaag agacaaccac tgtcattgtt
     9601 gcccagaaga tttcttctgt tgtcaaagct gataagattt tggttcttga cgaaggtcgt
     9661 ctgatcggcc aaggtaccca cgccgagctg gtagctacga atgatgtcta ccgtgaaatc
     9721 tacgaaacac agaaagggag ggaggaaaac taagcaagtg cagtctatat agtacgattg
     9781 tatttgaact cggtcggaaa gcttcgaaaa aagataaacc gcctagcgtt tcggaacgcg
     9841 gcgttggttt cctatttttc ggtcgctttc tgggcgacct tagtatcatg ttatgaagac
     9901 gttacgtttt ttctggtttt attttaaacg ctataaactg tcctttgctg tgatttttct
     9961 agccattgtg gcagcgacct acttgcaggt taagacacct gttttccttg gaaatgccat
    10021 tgcggagatg gggaaaatcg ggcaggctta cttcatggcc aatcaagctg gtcagactga
    10081 ctttcagcca gacatggctg attttaacgg ggttatgctc aatcttttct ttgcctatgc
    10141 ggcgacggtt gtggcttcct tgatttatac cctcctcttc acgcgtatcg tggctcattc
    10201 gaccaaccgt atgcgtaagg gcttgtttgg taagttggaa cgcttgacag ttgccttctt
    10261 tgatagtcac aaggacgggg atattctttc ttgttttacc agtgatttgg acaatatcca
    10321 aaacgctttt aaccagtcct tgacccaagt ggtgaccaac atcgctcttt atgttggtat
    10381 ggtcatcatg atgttccgtc aggatactcg cttggccttg gtgaccattg cttctacgcc
    10441 agttgccttg attgccttgg tcgttatcat ccgcctatca cggaaatata cggataagca
    10501 acaggctgcg gtgtctaagc ttaatgccta tatggatgag aagatttccg gacaaaaagc
    10561 gattattgta cagggagtgc aggaagagac gattgatggt ttcttggaac tcaatgaaga
    10621 agttcgccgt acgaccttca agggacgctt gtttggtggt attctcttcc catttatgaa
    10681 tggaatgagc ttggtcaata cggccattgt tatctttgca ggctctagca ttgttctcaa
    10741 tgatagctcg ctggaaacag ctgccgcact tggtttggtg gtgacctttg ttcagtattc
    10801 gcaacagtat taccagccta tcatgcaggt tgctgccagc tgggcagaat tgcagctagc
    10861 tttcacagga gctcatcgta ttcaggaaat gtttgatgag cctgaggaag ttcgccctca
    10921 aaacgctccg ctatttaccg aattaaaaga aggtgttgaa attaaggaca tcgactttgg
    10981 ctatctgccg ggtcagaagg tcttggacaa ggtgtctatc tctgctccta agggtaaaat
    11041 ggtggcagtc gttggtccga cgggatctgg taagactacc attatgaact tgattaaccg
    11101 cttctatgat gtcaatggtg gtagcgtggc ttttgatggt cgtgatattc gggaatatga
    11161 tctggacagc ttgcgggata aggtcggtat tgtcttgcag gagtcagtgc tattctcggg
    11221 tactattgcg gataatattc gctttggcga tgagagcatt tcgcaggaaa tggtggaaac
    11281 cgcagcccgt gccacccata tccacgactt tatcatgagc ttgccagagg gctatgaaac
    11341 cttcgtgacc gatgatgaga atgtcttctc tacaggtcag aaacagctga tttccattgc
    11401 ccgtacgctc ttgacagatc cacaagtctt gattttggac gaagcaacct caaacgttga
    11461 taccgtgact gaagctaaaa ttcaaaaagc tatggaggcc attatcgcag gacggaccag
    11521 cttcgtcatt gcccaccgcc tcaaaaccat tctcaatgcg gacgaaatca tcgtcctcaa
    11581 agacggaaaa gtgattgagc aaggcaacca tagccagctc ctcaaacagg gcggcttcta
    11641 cgccgaactc taccacaacc agtttgtgtt tgagtagcac acttcgaaaa tcacagacta
    11701 tttcattgaa taatagatgg tataagaatg tgaacagcaa aaaccagtca atctctattt
    11761 ctgtgattga ctggttttta ataatcttcg aaaatcaaaa tcagacgttg ttggcttgat
    11821 ttgatgagtg aaaggctcca gtggagcctt tcagcctgtt cccttgaaac aaaatagtaa
    11881 cagggttcta tctgcgtaag tagaaacgaa ctacgttcgc tctatctcca acctccaaag
    11941 gttcccaaac ctttggagct agtctgtt
//
LOCUS       DATUCG010000077        11800 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.078, whole genome shotgun sequence.
ACCESSION   DATUCG010000077 DATUCG010000000
VERSION     DATUCG010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11800
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.078"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(108..779)
                     /locus_tag="VCA20_001579"
     CDS             complement(108..779)
                     /locus_tag="VCA20_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin lysis protein"
                     /protein_id="HEP1835381.1"
                     /translation="MKTFISKLILVSTVVLLAACQSNQVKGGESSSSSASSQVLDGSS
                     LQQASIIASSSEMQASSVTTPKLSEVYQSVIDHYQANMVQPAEAINQDEVSSYLSLLT
                     SQGQEYSGIFYSLYDVNHDGTEELLLALDKSGEYVLIDLYTQLADESLRLVDNFRNLG
                     FEIGPNALLRPLQDGTYLFEGAGVFRIYQYNAMIPGLKRISESDTNPETSPLLELKSL
                     HWVKL"
     gene            complement(1061..1414)
                     /gene="rbfA"
                     /locus_tag="VCA20_001580"
     CDS             complement(1061..1414)
                     /gene="rbfA"
                     /locus_tag="VCA20_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEP1835382.1"
                     /translation="MANHFRTDRVGMEIKREVNEILQKKVRDPRVQGVTITDVQMVGD
                     LSMAKVYYTIMSNLASDNQRAQTGLEKATGTIKRELGRKLTLYKIPDLVFEKDQSIEY
                     GNKIDQMLRALDQKD"
     gene            complement(2039..4855)
                     /gene="infB"
                     /locus_tag="VCA20_001581"
     CDS             complement(2039..4855)
                     /gene="infB"
                     /locus_tag="VCA20_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEP1835383.1"
                     /translation="MSKKRLNEIARELGVSSKEVVAKAQELGFEVKSHASSVDEASAK
                     RLAESLGGQKSEETKVAAKVSKPEKVDETPKVETAKVEKAKETQPVVKEEVAASTVQS
                     APHRPQSRNFKAEREARAKEQAAKRAQGKGGRAKSGQDRRDNRQQGRGRSNNERNDRR
                     DNRRDQRPEERKDNRFGERRDNRANRRQDNRSGQSARFEQREAAKPAGPKIDFKARAA
                     ALKAEQNAEYARTSEERFRQAQEAKKQPKKPKEIKFEEPVVESKPFVKPAPVASVPEQ
                     VAETTVDTRRKKQARPDKKRDFNSDEEDGPRKQQRNRNSQNQVRNQRTSNWNNNKKNK
                     KGKANQPAKPVTERKFHELPTEFEYTAGMTVAEIAKRIKREPAEIVKKLFLMGVMATQ
                     NQSLDGDTIELLMVDYGIEAKEKVEVDNADIERFFVEEGYLNEEEMTERPPVVTIMGH
                     VDHGKTTLLDTLRNSRVATGEAGGITQHIGAYQIEEAGKKITFLDTPGHAAFTSMRAR
                     GASVTDLTILVVAADDGVMPQTIEAINHSKAANVPIIVAINKIDKPGANPERVIGELA
                     EHGVISTAWGGESEFVEISAKFNQNIDELLETVLLVAEIQELKADPTVRAIGTVIEAH
                     LDKGKGAVATLLVQQGTLNVQDPIVVGNTFGRVRAMTNDLGRRVKTVGPSTPVSITGL
                     NEAPMAGDHFAVYEDEKSARAAGEERAKRALLKQRQATQRVSLENLFDTLKAGEVKSV
                     NVIIKADVQGSVEALASSLQKIEVEGVRVNIVHSAVGAINESDITLAEASNALVIGFN
                     VRPTAEARSQAEADDVEVRLHSIIYKVIEEMEDAMKGMLDPEYEEKIIGEAIIRETFK
                     VSKVGTIGGFMVVRGKVTRDSSVRVIRDGVVVFDGKLASLKRYKDDVKEVGNAQEGGL
                     MIENYNDLKVDDTIEAYIMEEIKK"
     gene            complement(4873..5211)
                     /locus_tag="VCA20_001582"
     CDS             complement(4873..5211)
                     /locus_tag="VCA20_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEP1835384.1"
                     /translation="MLIIRSRGENLDSNKRQKILNLLGLAQRAGRLVSGEDLVVEAIQ
                     KGQAKLVFLAEDAAGNLSKKVTDKSHTYQVEVVTVFSTLELSAAVGKARKVLAVTDAG
                     FTKKMRSIME"
     gene            complement(5168..5464)
                     /locus_tag="VCA20_001583"
     CDS             complement(5168..5464)
                     /locus_tag="VCA20_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006531117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEP1835385.1"
                     /translation="MAKARKIPLRKSVVSGEIIDKRDLLRIVKNKEGQVFIDPTGKAN
                     GRGAYIKLDNDEASQAKKRRVFDRSFSMEVAEEFYDELIAYVDHKIKRRELGLE"
     gene            complement(5481..6623)
                     /gene="nusA"
                     /locus_tag="VCA20_001584"
     CDS             complement(5481..6623)
                     /gene="nusA"
                     /locus_tag="VCA20_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEP1835386.1"
                     /translation="MSKEMLEAFRILEEDMGINKADIIDAVTESLRSAYKRRYGQSES
                     AVIEFDEKKGDFHVFTVREVVDEVFDSRLEISLKDALAISSAYEMGDKIKFQEDPAEF
                     GRVAAQSAKQTIMEKMRKQKRAITFNTYKQHENEIMSGTVERFDNRFIYVNLGTIEAQ
                     LSKQDQIPGEVFQSHDRIEVYVYKVEDNGRGVNVFVSRSHPEMIKRLMEQEIPEVYDG
                     TVEIMSVAREAGDRTKVAVRSHNPNVDAIGTIVGRGGSNIKKITSKFHPARYDAKNDR
                     MIPTEENIDVIEWVADEAEFIYNALAPAEVDQVLFDTEDGKHATVVVPDDKLSLAIGR
                     RGQNVRLAAHLTGFRIDIKSASEYEAFEATQYATDEVVEEVAEEQE"
     gene            complement(6697..7167)
                     /gene="rimP"
                     /locus_tag="VCA20_001585"
     CDS             complement(6697..7167)
                     /gene="rimP"
                     /locus_tag="VCA20_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEP1835387.1"
                     /translation="MSSIIELVTAVITPAIQTPYELVDVEYGKMGGDYVLSIFVDKEG
                     GISLQDTADLSEKISPILDTIKPDPFPDQYMLEVTSPGLERPLKTVDAVEKAVGKYIH
                     VKLYQAIDKIKVFEGTLLSFDGTDLIMEYMDKTRKKEVTIPYQTVAKARLAVKL"
     gene            complement(7425..8063)
                     /gene="trmB"
                     /locus_tag="VCA20_001586"
     CDS             complement(7425..8063)
                     /gene="trmB"
                     /locus_tag="VCA20_001586"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEP1835388.1"
                     /translation="MRVRNRKGASELLANNPQYVISNPEECKGKWAEIFGNNNPIHIE
                     VGSGKGRFVTGMAAQNPDINYIGIDIQMTVLSYALDRVLEAGLPNIKLLQVDGSSLAN
                     YFAPAEIDQLYLNFSDPWPKKRHEKRRLTYKSFLDTYKEILPEKGEIHFKTDNRGLFE
                     YSLASFSQYGMVLKQVWLDLHADGLEGNVMTEYEEKFSNKGQVIYRVEAQFQ"
     gene            complement(8060..8854)
                     /locus_tag="VCA20_001587"
     CDS             complement(8060..8854)
                     /locus_tag="VCA20_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEP1835389.1"
                     /translation="MQFDTSGLQLQSIAGNSGKAFKGLRSDGTPVFVKYEMPPIVSAL
                     AREQITPPVLSANREVGMGHRVEQEWLNGRTLERQDMTSKQIRQILVRMHYSRILLNQ
                     ALQMNYTYMEPQDLVYRWQKEAPTRLGQNSYLQSICQDLLNNLPRFRQEVATFVHGDL
                     HHSNWVETTSGLVYLTDWETACVTDRMLDVAYILTHYIPRQSWEEWLRAYGYKYNSTV
                     LNKVYWYGQLGYLNQIAKHVESYNVLAANKEIYALRQFRQSFYKEV"
     gene            complement(8904..9944)
                     /locus_tag="VCA20_001588"
     CDS             complement(8904..9944)
                     /locus_tag="VCA20_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP1835390.1"
                     /translation="MKELFQQRRLDFLNRCSKYLRYVLNDHFVLVLMVLLGFLSLQYR
                     QLLLSFPENPLPIVLVVIAISLLVLLSGRIATYLEEADQIFLLTKEKDILQGLEVAAR
                     RTVLFWSSIQVVVQLVLLPIYLKLGLPVWMIVAGLLTLLVLKCLFVKRQLVAYQSQGV
                     LDWSKAIQDEQKRKQSILRFFALFTTVKGISTTVKPRSYLNGILRLVNSRQTWFYLYL
                     RAFLRSGDYLGLTIRLLLLAILSLFAIEESWLSIGLVLVFHYLLVFQLLGLYKHYDYQ
                     YVTQLYPLEKDSKKIGLQNLLRVILYSLLVVEFGFALLFSKENAMVVVLLLVGIFLHE
                     IYLPLKLKKLID"
     gene            complement(9941..10675)
                     /locus_tag="VCA20_001589"
     CDS             complement(9941..10675)
                     /locus_tag="VCA20_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1835391.1"
                     /translation="MLEVKNVTGGYVNIPVLKDVSFTVEDGQLVGLIGLNGAGKSTTI
                     KEIIGLLTPYQGQIAIDGLTLLQGAENYRKKIGFIPETPSLYEELSLKEHLEVVAMAY
                     DLSWEQAWGRIEHLLKLFRLDEKLDWFPVNFSKGMKQKVMIICAFMVEPSLLIVDEPF
                     LGLDPVAISDLIALLEEEKSKGTSILMSTHVLDSAEKMCDSFVILHQGQVRATGNLEE
                     LQSAFGMDGASLNEIYLALTQEGVAG"
     gene            10738..11151
                     /locus_tag="VCA20_001590"
     CDS             10738..11151
                     /locus_tag="VCA20_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEP1835392.1"
                     /translation="MSDCIFCKIISGEIPASKVYEDDQVLAFLDITQVTKGHTLVVPK
                     KHYRNVLGMDEEAAATLFSVVPTIARQLKEKLGASGLNIVNNNEEAAGQTVFHTHIHL
                     LPRFNNQDGLSIQFKANDPDFPALDQLAQELYLGE"
     gene            11153..11456
                     /locus_tag="VCA20_001591"
                     /pseudo
     CDS             11153..11456
                     /locus_tag="VCA20_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027753.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
     gene            complement(11615..11800)
                     /locus_tag="VCA20_001592"
                     /pseudo
     CDS             complement(11615..11800)
                     /locus_tag="VCA20_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010579648.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3338 a         2788 c         2101 g         3573 t
ORIGIN      
        1 ccttttcttc ctaaaataaa gattaaagat tgccaaaaga aaacttaaag ttcttaggaa
       61 ctacaaaagg ccgggaaacc cagcctagga ataatcattg ataaaatcta tagcttaacc
      121 caatgtaagc ttttcaactc aagcaaggga gaagtttcag gattggtatc tgattcagaa
      181 atccgtttta aacctggaat catcgcattg tattgataaa tgcgaaaaac accagctcct
      241 tcaaataagt atgttccatc ctgtaaagga cgaagtaggg cgttcggtcc aatttcaaat
      301 ccaagattgc ggaaattatc caccaaacgt agactctcat cggcaagctg agtatagaga
      361 tcaattaaga catattctcc cgacttgtcc aaagctagaa gcagctcctc tgtaccatca
      421 tggttgacat catacagact ataaaatatt ccactgtatt cttgcccctg gctagttaat
      481 aagctcagat agctactgac ctcatcctga ttgattgcct cagcaggctg caccatattc
      541 gcttgataat gatcaataac tgattggtat acttcactta gcttaggagt ggtcactgag
      601 gatgcttgca tctcggagct agatgctatt attgacgctt gctgcaaact agaaccatct
      661 aaaacttggc ttgaagcact agaactagaa ctctcaccac cttttacctg attagactga
      721 caagcagcta atagtacaac cgtggacact aatatcagtt tactaataaa tgttttcata
      781 acttcctcct gttctctatc tttggtctta tactcttcga aaatcaaaat tatctgttgt
      841 caacttacct tgatgagtga aaagctccag tggaggtttt cagcctgtta ccttgaaata
      901 agatagtaac agagttctat cttcggcttc gtttcctagg ctacttttga ttttcattga
      961 gtattagtat aacatctttc cgagagcttg taaatgccac catatatgga caaagcatcc
     1021 gagccagaaa aagccgaatg ttcccatccg acttttgttg ttaatctttc tgatcaagag
     1081 cacgcaacat ttgatcaatt ttattgccgt actcaattga ttggtctttt tcaaagacca
     1141 aatccggaat tttataaaga gttaacttac gaccaagttc tctcttaatg gttcccgtcg
     1201 ctttttcaag acccgtttgt gctctttgat tatcggatgc caaattactc atgattgtgt
     1261 agtaaacctt ggccattgac aaatcgccga ccatctgaac atctgtaatg gtcactccct
     1321 gcacacgtgg atcacgaact ttcttttgca aaatctcatt gacttcgcgc ttgatttcca
     1381 tcccgacacg gtctgtacga aaatgattcg ccatattctt tcctttctta gatgatagaa
     1441 aagctgagtt tccccagctt gaataatcca agtagatcaa aaactattgc tttaaaatct
     1501 actaaagtct attatagtct tttagtttac ttttaatacg aggcaatggc cgcaggcata
     1561 actcaatgct ttcttatttc taggcattga cttgaaacag tcccccagat tgcttgcaca
     1621 gtcaatggac tgtgcaaggt gggagataat acttgcaaag caagtcactt ttacaaaatc
     1681 aggcaaggcg agttcaaaca atccagtgga gtgtttgaag ttggaaatag aggctgagca
     1741 aaaagcccag cgccactttt tagagttcgt gtcaccatct cagcgcagtg gttgattggg
     1801 tttaacagtc cagtggagtg agacagttcg gggaactgtt tcagctggtc acctaaaaat
     1861 aagaaagtga ccatagagaa ccgagccaac tgcttggaag tgaggggaac tctttttaga
     1921 ccactcgagt tctttcccac tccccttacg tcgaagtaat aaaagaaaaa ctaaatgacg
     1981 ataaattgtc acttcctcga caaaatcact tgtctatgac tttgacagtg agaaaatatt
     2041 attttttgat ttcttccatg atgtaagctt caatggtatc atcaactttc aagtcattgt
     2101 agttctcaat catcaaacca ccctcttggg cattaccaac ttctttcacg tcatctttat
     2161 agcgtttcaa actagctagt ttaccatcaa atactacaac accatcacgg ataactcgaa
     2221 cacttgaatc acgggtaacc ttaccacgta cgaccatgaa accaccgatc gttccaactt
     2281 tggatacctt gaaggtttca cgaatgattg cctcaccaat gattttttct tcatattctg
     2341 gatcaagcat acctttcatg gcatcttcca tctcttcgat aaccttgtaa atgatgctgt
     2401 gaagacgtac ttcaacatca tctgcttcag cttggctacg cgcttcagct gttggacgaa
     2461 cgttgaaacc aatcacaaga gcatttgaag cttccgcaag agtaatatct gattcattga
     2521 tagcaccaac tgcagagtgg acgatattga cacgtactcc ctctacttca atcttttgaa
     2581 gcgatgatgc aagagcttca actgaacctt gtacgtcggc cttgataatc acattaactg
     2641 acttaacctc acctgcttta agggtatcaa agaggttttc aagactaact cgttgcgttg
     2701 cttgacgctg tttaagaaga gcacgtttag cacgttcttc acctgctgca cgagctgatt
     2761 tctcatcctc gtaaactgca aagtgatcgc ctgccatagg agcttcgttc aaaccagtaa
     2821 tagataccgg tgtagatgga ccaacagttt taacacgacg accaaggtcg tttgtcatgg
     2881 cacgaacacg tccgaaggta tttccgacaa cgattgggtc ttgaacatta agtgtccctt
     2941 gttgaacgag gagggttgca accgcacctt tccctttatc caaatgcgct tcgataactg
     3001 taccaatcgc acgtacagtt gggtctgcct tcagttcttg gatttcagca accaaaagaa
     3061 cagtttccaa caattcatca atgttttggt taaacttagc tgagatttct acaaattcag
     3121 attctccacc ccaagcggtt gagataacac cgtgctcagc caattcaccg atgacacgct
     3181 ctgggtttgc acctggcttg tcaatcttgt tgatagctac aatgattggc acattagcag
     3241 cttttgagtg gttgatagcc tcaatcgttt gtggcatgac accatcatct gcagcaacga
     3301 ctaggatggt caagtcggta acagatgcac cacgtgcacg catagaagta aaggccgcgt
     3361 gtcctggagt atccaagaag gtaattttct taccagcttc ctcaatttgg taggcaccga
     3421 tatgctgtgt gataccacca gcttccccag tagcaacacg agaattacgt agggtatcca
     3481 acaaggttgt tttaccatgg tcaacgtgtc ccatgatagt cacaacaggt gggcgttctg
     3541 tcatctcttc ttcattgagg tagccctctt ctacgaagaa acgctcaata tctgcgttat
     3601 cgacctctac cttttctttc gcttcaatac cataatcaac catgaggagt tcgatagtat
     3661 ctccatccaa agattggttt tgagtcgcca taactcccat aaggaagagt tttttcacaa
     3721 tttcagcagg ttcacgtttg attcgttttg caatttctgc gacggtcatg ccagcagtgt
     3781 attcaaattc ggttggcaat tcgtggaact tgcgttctgt gacaggtttg gcaggttggt
     3841 tagccttgcc ttttttattt ttcttgttgt tattccagtt acttgttctt tgatttctca
     3901 cttgattttg actatttcga tttctttgtt gtttccgtgg accatcttct tcgtcactat
     3961 taaagtcacg ttttttatct ggtcgagctt gtttcttacg acgggtatct acagtagttt
     4021 ctgcaacttg ctctggtacc gatgcaacag gtgcaggctt cacaaatggt ttgctttcca
     4081 caaccggttc ttcaaactta atttcttttg gcttcttagg ttgcttttta gcttcctgag
     4141 cctgacgaaa acgttcttca ctggtacgag catactccgc attttgttct gctttcaaag
     4201 cagcagcacg cgctttgaag tcaattttag gtcctgctgg ttttgcagcc tctctctgtt
     4261 caaagcgagc tgactgacct gaacggttgt cctgacgacg gttggcacga ttatcgcgtc
     4321 gttcaccaaa tcggttgtct ttccgctctt ctggacgctg gtcacgacga ttgtcgcggc
     4381 gatcatttcg ctcattgttt gaacgtcccc gaccttgttg acggttgtca cgacggtcct
     4441 gaccagattt agctcgacca cctttacctt gtgctcgctt ggctgcttgt tccttagcac
     4501 gcgcttcccg ttctgcctta aagttacggc tctgtggacg atgtggagct gattgtactg
     4561 tacttgccgc aacttcttct tttactactg gctgtgtttc cttagctttt tctaccttag
     4621 ctgtttccac ttttggagtt tcatcaacct tctcaggctt gctaactttt gccgcaactt
     4681 tagtttcctc tgatttttgt ccccccaagc tttctgcaag gcgtttagca cttgcttcat
     4741 ctacgcttga agcatgactt ttgacttcaa aacccaattc ttgagcctta gcaacaactt
     4801 ccttactgct tacgcctaat tcacgggcta tttcattcaa tctcttctta gacaatgcct
     4861 tgtcctcctg ttctattcca taatagacct catcttcttt gtaaatccag cgtctgtcac
     4921 tgcaagaact tttctagcct tgccgacagc tgcactcaat tccagtgttg aaaacactgt
     4981 tacaacttct acttgatagg tatgactttt atctgttact tttttactta aatttccagc
     5041 agcatcttcg gctaaaaaca ccaactttgc ctgccctttt tgaatagctt caacaaccag
     5101 gtcctcaccc gaaaccaggc gaccagctcg ctgggccaaa cctaataaat ttaagatttt
     5161 ctgtctctta ttcgagtcca agttctctcc tcttgatctt atgatcaaca taggcaatta
     5221 attcatcgta aaattcttcg gccacttcca tactaaagga acgatcgaag acacgacgtt
     5281 tcttagcttg gcttgcctca tcattgtcaa gcttgatgta agctccacga ccatttgcct
     5341 tgcctgttgg atcgataaat acttgacctt ctttattttt tacaatacgg agcaaatccc
     5401 gtttgtcgat gatttcaccg gacaccacag acttgcgcaa gggtattttt ctagcttttg
     5461 ccatggttgc tccttttgcc ttattcttgt tcttctgcga cttcttctac aacttcgtct
     5521 gttgcatatt gagttgcttc aaaagcttca tactccgatg ctgacttgat gtcaatacgg
     5581 aatcctgtca aatgcgctgc caaacgaacg ttttgaccgc gacgaccaat tgccaatgac
     5641 aacttatcat ctggtacaac gactgtagca tgtttcccat cttccgtgtc aaacaatact
     5701 tggtcaactt cagcaggtgc aagggcattg tagataaatt ccgcttcatc tgctacccac
     5761 tcgatgacat caatattttc ctcagtagga atcatacggt cattcttagc atcataacga
     5821 gctgggtgga atttgctagt gattttcttg atatttgaac caccacgacc aacgatcgta
     5881 ccgatggcat ctacgtttgg attatggcta cgaacagcca ctttcgtacg gtctccagct
     5941 tcacgagcaa cactcatgat ttcaactgtt ccatcgtata cttctggaat ttcctgttcc
     6001 atgagacgtt tgatcatttc tggatggcta cgactgacaa atacgttcac cccacggccg
     6061 ttatcttcta ccttgtaaac atagacttcg atacggtcat gtgattggaa aacctcgcca
     6121 ggaatttggt cttgttttga caattgtgct tcaatcgtac caaggttgac atagataaag
     6181 cgattatcaa aacgctctac cgtacctgac ataatctcat tttcatgttg cttgtaagta
     6241 ttgaaggtaa tggcacgctt ttgtttgcgc atcttttcca tgatggtctg tttagctgat
     6301 tgagcagcta cacgtccaaa ttccgctggg tcttcttgga atttaatttt gtcacccatt
     6361 tcataagctg atgaaatagc caaggcatct tttagactaa tttctagtcg gctatcaaat
     6421 acttcatcta ctacttcacg aacagtaaat acatggaaat cgcctttttt ctcgtcaaat
     6481 tcaatcactg ctgattctga ttgaccataa cggcgtttat aagctgaacg aagcgactct
     6541 gtaactgcat caatgatatc cgctttgtta atacccatgt cttcctctaa aatacggaag
     6601 gcttctagca tttctttact catgttgttt tccttttgcg ctcaaaaggt cctttcttaa
     6661 agtctaatat ctttataaaa tactcatgaa agagcattat aacttaacag ccaagcgggc
     6721 cttagccacc gtctggtaag gaatagtcac ttccttctta cgagttttat ccatatactc
     6781 cataatcagg tctgttccat caaaggaaag caacgttcct tcaaaaacct tgattttatc
     6841 aattgcctga tagagcttga catggatgta ctttccaacc gccttctcaa cagcatctac
     6901 cgtttttaaa ggacgttcca aacctggact tgttacctcc agcatgtatt ggtctggaaa
     6961 tgggtctggt ttgatagtgt ccaaaatcgg actgatcttt tcggataaat ctgccgtatc
     7021 ttgaagggaa attccaccct ccttatcgac aaaaatcgat aggacatagt caccgcccat
     7081 tttaccatat tccacatcta ccaactcgta aggagtttga atagcggggg taattactgc
     7141 tgtgaccaat tcaataattg atgacataaa cacctcctat ttttacagtc gctagaaata
     7201 cattactcaa atagttcgca ctatccagga ctctctattt cctttgacag taaactccct
     7261 tgtactatca ttcattctat atacagaaat ccgtgtacag ggaaagggtt gactgacgcc
     7321 aacccctttt ctcattattc acttccatta ttctatcata tctatcccta tttgtaaagg
     7381 aaaaaggtct ctgttagacc tctcctcatt ttccactaag agaattattg aaactgtgct
     7441 tccacacgat agatgacttg acccttgttt gagaatttct cctcatactc tgtcataacg
     7501 ttgccttcca aaccatcagc atgcaaatct agccaaactt gcttcaagac catgccatat
     7561 tgagaaaaac tagccaggct atactcaaac agaccacgat tatctgtttt gaagtggatt
     7621 tctcccttct caggtaggat ttccttgtag gtatccaaga atgacttgta ggtcaaacgg
     7681 cgtttctcat gacgtttctt aggccatgga tctgaaaaat tcagataaag ctgatctatt
     7741 tctgccggtg caaaatagtt ggccaaactc gaaccatcca cctgcaataa ttttatattt
     7801 ggtaagccag cttcaagcac acggtccaag gcatagctta acacggtcat ctgaatatca
     7861 ataccaatat agttaatatc tggattttgc gcagccatac ctgtgacaaa gcgaccttta
     7921 ccagaaccaa cttctatgtg gataggatta ttgtttccaa aaatctctgc ccatttcccc
     7981 ttacactcct ctggattgga gataacatac tggggattgt ttgctagaag ctcactcgct
     8041 cccttacgat ttctaactct catacttcct tatagaaact ctgacggaat tgccgtagag
     8101 cataaatttc tttatttgca gccaaaacat tgtagctttc tacatgctta gctatttggt
     8161 ttagatagcc aagctggcca taccaataaa ccttatttaa tactgtgctg ttatatttgt
     8221 agccataagc ccgaagccac tcttcccagg actgacgagg aatataatgc gtcaaaatat
     8281 aggcaacatc caacatccga tccgttacac aagcggtctc ccaatctgtc aaatacacta
     8341 aaccagacgt cgtttctacc cagttactat gatgcaaatc tccgtgaaca aatgttgcca
     8401 cctcttgacg gaatcttggt aaattattca ataaatcttg acagatagat tgcagataag
     8461 aattctgtcc caatctagtt ggcgcctctt tttgccaacg ataaaccaaa tcctgaggtt
     8521 ccatataggt ataattcatc tgcaaagcct gattgagcaa aatgcgagaa tagtgcattc
     8581 tcaccagtat ttgacgaatc tgtttactag tcatatcttg acgctccaag gtacgaccat
     8641 tcaaccactc ctgctctaca cgatgcccca ttcctacttc acgatttgct gacaaaacgg
     8701 gtggtgtaat ttgctcacgc gcaagagcag acactatcgg aggcatctca tacttcacaa
     8761 agactggcgt tccgtctgac cgtaggcctt taaacgcttt gccactattt cctgcaatag
     8821 actgtaattg tagcccactg gtatcaaact gcatgccttt cctcctttaa aaattcctta
     8881 ctattttact aattttcgtc tatttagtca atcaattttt ttaattttag aggcaaatat
     8941 atttcatgca agaaaatccc cacgagaagt aagacgacta ccatcgcatt ctctttagaa
     9001 aatagcagag caaagccaaa ttccactact aatagactgt ataaaattac tctaagcaaa
     9061 ttctgaagac cgattttctt ggaatctttc tccagaggat acagttgtgt tacatattga
     9121 taatcataat gcttataaag ccccaacaac tggaagacaa gcaaataatg aaagactaaa
     9181 actaggccga tggataacca cgattcttca atggcaaata gggacaaaat cgccaacaac
     9241 agtagacgta tcgtcaagcc caagtagtct cctgaacgca agaaagcccg taagtagaga
     9301 taaaaccaag tctgtctcga attgacaaga cgcaagattc cattgagata gcttctcggt
     9361 ttaacagtcg tactgattcc tttaaccgtc gtaaataagg caaagaaacg taaaatagac
     9421 tgtttccttt tttgttcatc ttgaatagcc ttagaccagt ccaatactcc ctgtgactga
     9481 taagccacta attggcgctt gacaaaaaga cattttaaaa ccaacaaagt aagaagacca
     9541 gctacaatca tccacaccgg taaacccagt ttcaaataga ttggtaacaa aacaagctga
     9601 accaccacct gtatagagga ccaaaacaag accgtgcgtc tggctgcaac ctcgagtcct
     9661 tgcagaatgt ctttttcctt ggtcaatagg aagatttgat ctgcttcttc taaataagtc
     9721 gcaattcttc cggacaatag caccagtaga ctgattgcaa taaccaccaa aacaatcggc
     9781 aacggattct cagggaatga caacaataat tgacggtatt gcaaactcaa aaagcctaat
     9841 aaaaccatga gaaccaaaac aaaatggtca ttcagaacat agcgcagata cttggaacaa
     9901 cgatttaaaa aatctagtcg gcgttgttga aacaattctt tcatccagcc actccttctt
     9961 gcgtcaaggc cagataaatt tcattcagac ttgcaccatc cataccaaat gcgctttgta
    10021 gctcctccaa atttccagtt gcacgaacct gtccctgatg taaaatcaca aaagagtcgc
    10081 acattttctc agctgagtca agaacgtgag tggacatgag gattgaagtg cccttagatt
    10141 tttcttcctc caaaagagcg atcaagtcag agatagccac agggtctaat cccagaaaag
    10201 gctcatcaac aatcaacaga ctcggctcca ccataaaagc acaaataatc atgaccttct
    10261 gcttcattcc ctttgaaaaa ttgacaggaa accagtccaa tttctcatcc aagcgaaaca
    10321 gttttaacaa gtgctcaata cgtccccaag cttgctccca ggataaatca taggccattg
    10381 ctaccacttc caagtgctct ttgaggctta gttcttcata caaacttggt gtttcaggga
    10441 taaacccaat cttcttccga taattttcag ccccttgtag caaagttagg ccgtcaattg
    10501 caatctgtcc ttgatagggg gtcaaaagac cgataatttc cttaatagtc gttgatttcc
    10561 ctgcaccatt taaaccaatt agaccaacta attgaccatc ctcaacggta aaagatacat
    10621 ctttcaaaac tgggatattg acatatcctc cagttacatt ttttacttct aacatacatt
    10681 tcttcctaca atttgatata attattataa caaaaatcct agaaagaggt tgccattatg
    10741 tcggattgta ttttttgcaa aattatctct ggagaaatac cagcttcaaa agtctacgaa
    10801 gatgaccaag tattagcctt tttagacatc actcaggtca caaaaggaca cacattggtt
    10861 gtccctaaaa aacactaccg aaacgtgctt ggcatggatg aagaagctgc agccacactc
    10921 ttttctgttg tgccaaccat cgctcgccag ttaaaagaaa aacttggagc tagtggtcta
    10981 aacatcgtta ataataacga ggaggccgct gggcaaaccg tctttcatac acacatccat
    11041 ctcttaccac gctttaacaa tcaagatggc ttgagcattc aatttaaagc caacgaccct
    11101 gacttcccag ccctggatca attggcacaa gagctctatc taggagaata atatgaaact
    11161 tcgtaatatc attttagccg taagcgccgc taccgccagc ttccttgcag tcacgcatcg
    11221 ggataaaatc gctaaagaag tacaggaaac caaacaactt ctatcagata ttcaactgag
    11281 caaggacaat attcaggaac aacttgccat tattcaaagt tttcaagaac ccttgcagga
    11341 aatggcttct gacttacaat ataagactcg agtctatcag caaagtattg caggaaactt
    11401 ggaagaaatc caacagattc tcaaaaaaat accagaatga gaatagcact ccctaacata
    11461 gggaatttcc aaatcatgac cacccgtcaa acgggtggtt tgaacaaggg ctataagccc
    11521 acatcacgag ccagcgccta aagacgctgg ctttcacttt gttcaagcct cactgctttt
    11581 gactcgtcac tagcctctta aagaggcatt cgtactactt gccattatcc ctaaagggat
    11641 cttcatactc ttttacactc aatttatcaa gtgccatatc atgtttaact cgctttctag
    11701 atttaagctc atgaagaaat catccactgg ataatttctt tgttcttgta tatatttctt
    11761 aattgtggct tcatttagtc caaccgtact tacataatat
//
LOCUS       DATUCG010000078        11761 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.079, whole genome shotgun sequence.
ACCESSION   DATUCG010000078 DATUCG010000000
VERSION     DATUCG010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11761
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.079"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            312..827
                     /locus_tag="VCA20_001593"
     CDS             312..827
                     /locus_tag="VCA20_001593"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835393.1"
                     /translation="MEFQARVSSECMYYISLLEEIYSKEITGAVTRGIVLSKAFEETK
                     NLNNWLKISEDTHTIPLHNIEYSKGYGVKIKAEINEKTDRGIRNLKIELPKYLPVRSV
                     TIGVTVKLICKAAILLRRDEKFRQTEILSVSEHFEHLEEKLKKIVAPVNYDKLQDILA
                     DSKNEIQRNVK"
     gene            1074..1355
                     /locus_tag="VCA20_001594"
     CDS             1074..1355
                     /locus_tag="VCA20_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835394.1"
                     /translation="MFQTLIAFIYSATDEQFQAFQTKANALTGNVTISQNVAPITDAL
                     KNRFGLKTIQTAFARKLVYASTRHHYKDGKTMMEDILDGKTRRHANSYI"
     gene            1421..1810
                     /locus_tag="VCA20_001595"
     CDS             1421..1810
                     /locus_tag="VCA20_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835395.1"
                     /translation="MTVIGFTPNPNALVLQLTETSVIARRIFLPRASPYFEILESKPF
                     DFGWEKNILVICAPTTASNNAFELLFPSMLPHATTGDFFILLSILDKQDGAIIAGTLG
                     LKDYATVRKNLRVRRNNKYLPIIWRGD"
     gene            2147..2599
                     /locus_tag="VCA20_001596"
     CDS             2147..2599
                     /locus_tag="VCA20_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835396.1"
                     /translation="MDISKSAQLQEANNRLETLVNVFNLNPNLLKYWNEGKLYYSYLT
                     GGGLLASIDTITYDPRYQEQVKQYEDRTGNLVYHVIESGSLLNLLYVSAPDKNISEDE
                     QREIWDMELMNQTFSWVSAYVINLDDPDLSEHGDILLGAYQGVLIRTE"
     gene            2656..3414
                     /locus_tag="VCA20_001597"
     CDS             2656..3414
                     /locus_tag="VCA20_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEP1835397.1"
                     /translation="MTQLTISTWNINQRSGIGEQIPDMVVTELRQLDTDIICLTEYVK
                     TENHNLFCTKLQDIGYEIFEDARSLGFGNEILIAIKSDLVNDSKVITIDNDDCNPNFL
                     QVRVTIKGKKLNIIGVRIKIGGSNIIEDFKERKVQLDNLISNLPASHEATIVLGDLNN
                     GFFKPSDDVHSYQGKAREFYSYPLLVKEMSESGLTAYTPSDVNSWKYCKLDHIFANVP
                     ISNETYSWKFIKNPDYKSQVGYPDHAILSATVSL"
     gene            3435..4754
                     /locus_tag="VCA20_001598"
     CDS             3435..4754
                     /locus_tag="VCA20_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="otitis media-associated H10"
                     /protein_id="HEP1835398.1"
                     /translation="MIKIKKVADLKRNYTISTSKLKIAHWSILGFSTCIMLTIIANIR
                     APDLIKIPLFILAGFVIYKFHRQLKYFRYHLDYYLIIRESLLYVLYTNRLYTAQKDST
                     GYEKIIRSAILEYELDRQKGHVLIKALITGDEFSKKVQSLDDVLAGVLELELDEKIIR
                     PSFVEYHFYYKKPERLILQSHSKKQMINNHNIDLGYGIIYDPVKCPHILVSGGTGSGK
                     SVFITFLILELLKRQSTVYIADPKNSDLGSLSHYFGEKYVATTPNNIARIVRLVVEEM
                     QERYQYMRDNFLYGSNFADHGFKPLWLIFDEMGAFQASGTDKKSREIIAEVMDGIKQI
                     ILLGRQSGIFILVAAQQMRAETLNTDLRDNLGLRIALGANSNEGYRMVFGSATPDNLK
                     PIEVKGAGYLYMQGSGRENAQYWESPYLDTKQFNFISELQLYLEETN"
     gene            4845..5159
                     /locus_tag="VCA20_001599"
     CDS             4845..5159
                     /locus_tag="VCA20_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835399.1"
                     /translation="MKLNDFLKPELLGNRFFAVKGYTEVLDRETNKPIALRLNVSIQD
                     ETSDFFMEMIQVKVNTLTPTASVQEMANNKTCPVALKDLNVGQFNGNLWFACSDVLPV
                     TK"
     gene            5201..5590
                     /locus_tag="VCA20_001600"
     CDS             5201..5590
                     /locus_tag="VCA20_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835400.1"
                     /translation="MQIFTYNDFLKDKGIITFEQAEKIYEDLISSVNIHDSEFLEYWQ
                     RLIELCAQYAEARGKFLTIPLAEKDSANKSRTRIHDSIIIQLKIIKRYAEKIGNDTSW
                     FDKFHDDRKRMGDFANYLNYVYAVNAR"
     gene            5600..5923
                     /locus_tag="VCA20_001601"
     CDS             5600..5923
                     /locus_tag="VCA20_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835401.1"
                     /translation="MIEDVNYYSQPPGKYSDSELQAIKDLLEVMIFFIDDTPHTNNKR
                     IWFEPLKYWENTNYIRFSHQIDFHSLHRIAEEDLSWEGGYKFTPQQIEDILKWMQTQE
                     WIRNE"
     gene            6138..6917
                     /locus_tag="VCA20_001602"
     CDS             6138..6917
                     /locus_tag="VCA20_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEP1835402.1"
                     /translation="MKTLLVGDLHLKSQLILPIVDNVIKDNNVKRVIFLGDYVDFPGQ
                     TNNINLYAHDLTFLFSWKKNMEANGLEVINIIGNHDADYLINFVSQYSVENIEVFFAV
                     KEQLKELGLQVAFQLNDFLISHAGYTTLFNPEPWHFEKITSRHLHKLEELVDSIGTKR
                     GGLFETGSPLWADLSELKEKTNLKYPKQIVGHTPQKNIDTSNNIIGIDTFSLYLNNHH
                     EYQVIGNGDLIIHDDKHNELTVISTNWGNLETYETLKKLIQ"
     gene            6927..7688
                     /locus_tag="VCA20_001603"
     CDS             6927..7688
                     /locus_tag="VCA20_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEP1835403.1"
                     /translation="MSKIYAMSDIHGHYDEFIESLSKVDLSDKNNQLILLGDYIDGGS
                     QSFQVISKIIELEMKFPDQVITLLGNHEEYFCEWLFEEDGASKYTSYDTIISFIGQNK
                     RKNIVDTVISDFSLSDEECLAQINKKIRDCVTSEYPEIVDWFRNKYFNSERFYETDTQ
                     IFVHAGIDEEFGENWKLYSNPDIFTNKFPASIGFFYKDIISGHIASSEVANDPSYLGK
                     IYYDQKSHYFIDGCVGKSHIIPILCYNTVTQSYNY"
     gene            7913..8695
                     /locus_tag="VCA20_001604"
     CDS             7913..8695
                     /locus_tag="VCA20_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HEP1835404.1"
                     /translation="MTSYNKKIVRTSSYIEIWEYDKPIFSKGKSITDTNTENKEKSKR
                     RTFDELTPEEQEQRLERMKKTRLEAKWSLLRLVDCNFDDTTSFLTLTTKENISERNEF
                     KDLLKTFIKRFNYHIFKTKKSQLRYIAVLEKQKRGAWHAHILLFSVPYIPHKKLLELW
                     GHGAVRINKVDVDSKENRGRYVTKYFEKGIGQELLENFGKQAYLSSRNLKKPDEDKFY
                     TYEVFNYDSSVVLYETEYTSKVYKDGQYFDNLVKYKKIKLDD"
     gene            8709..8915
                     /locus_tag="VCA20_001605"
     CDS             8709..8915
                     /locus_tag="VCA20_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEP1835405.1"
                     /translation="MIATVNKNDLVALGFSEGTSKRIIRQGKELLIARGFRVYQNKRI
                     GTIPASIATELLGFDVQNSSLSRG"
     gene            8919..10052
                     /locus_tag="VCA20_001606"
     CDS             8919..10052
                     /locus_tag="VCA20_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEP1835406.1"
                     /translation="MAIRKNKDGGWIVDISNGFDPITLKQRRVVRKGYKTKKEATEAE
                     HYLRSVELKERFYGAKITVSMLYELLKEEDSINCRKISYINTQDNNYNRHIKDYFSKV
                     DNVGKLTYEDIYQFREHLKQKVAQNSNKPLNTNTINKIMILLKKIFDVGLRKGYYATN
                     PVKLLKKLPIEKTKMQFWTVEEFQQFLTLLEPEEYNIKLLFTVLFFTGLRLGEALALT
                     WQDIDFSSNTIHITKSIYVNKGISHISTTKTKAGTRRIIINKKLSQELQHWQQQQKHL
                     LGQFTSDSLTLQVFQSNPLTITKNAIEKQYKKILERDDTLKKIRIHDFRHSHASLLIN
                     QGEDYLVVKERLGHASITTTIDTYSHLYPSKQKALADKLDDLL"
     gene            10116..10667
                     /locus_tag="VCA20_001607"
     CDS             10116..10667
                     /locus_tag="VCA20_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835407.1"
                     /translation="MKHNFEVKILIKENLDEDDKTYIQKVINKLKKRYGIIQEGNIHY
                     RNEVEKFDDYPYSVSFALRLSRYTRYFETFQFNNIAIGEYNRMEFEPKVWKESHNAEV
                     VIFLKQDLSEEEEKYILNEIEKYKKELGIIQDGNLFYRNERKLLDDFQFILKFGYMMS
                     KYKKYFEVLYLNSMAEGIYNHEL"
     gene            complement(10803..10886)
                     /locus_tag="VCA20_001608"
     tRNA            complement(10803..10886)
                     /locus_tag="VCA20_001608"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10850..10852),aa:Leu,seq:caa)
     gene            complement(10898..10969)
                     /locus_tag="VCA20_001609"
     tRNA            complement(10898..10969)
                     /locus_tag="VCA20_001609"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10935..10937),aa:Gln,seq:ttg)
     gene            complement(10981..11053)
                     /locus_tag="VCA20_001610"
     tRNA            complement(10981..11053)
                     /locus_tag="VCA20_001610"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11018..11020),aa:His,seq:gtg)
     gene            complement(11061..11131)
                     /locus_tag="VCA20_001611"
     tRNA            complement(11061..11131)
                     /locus_tag="VCA20_001611"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11097..11099),aa:Trp,seq:cca)
     gene            complement(11139..11219)
                     /locus_tag="VCA20_001612"
     tRNA            complement(11139..11219)
                     /locus_tag="VCA20_001612"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11183..11185),aa:Tyr,seq:gta)
     gene            complement(11240..11312)
                     /locus_tag="VCA20_001613"
     tRNA            complement(11240..11312)
                     /locus_tag="VCA20_001613"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11277..11279),aa:Phe,seq:gaa)
     gene            complement(11328..11401)
                     /locus_tag="VCA20_001614"
     tRNA            complement(11328..11401)
                     /locus_tag="VCA20_001614"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11365..11367),aa:Met,seq:cat)
     gene            complement(11411..11500)
                     /locus_tag="VCA20_001615"
     tRNA            complement(11411..11500)
                     /locus_tag="VCA20_001615"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11462..11464),aa:Ser,seq:tga)
     gene            complement(11507..11578)
                     /locus_tag="VCA20_001616"
     tRNA            complement(11507..11578)
                     /locus_tag="VCA20_001616"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11543..11545),aa:Glu,seq:ttc)
     gene            complement(11585..11658)
                     /locus_tag="VCA20_001617"
     tRNA            complement(11585..11658)
                     /locus_tag="VCA20_001617"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11622..11624),aa:Ile,seq:gat)
     gene            complement(11689..11759)
                     /locus_tag="VCA20_001618"
     tRNA            complement(11689..11759)
                     /locus_tag="VCA20_001618"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11725..11727),aa:Gly,seq:tcc)
BASE COUNT         4235 a         2000 c         2088 g         3438 t
ORIGIN      
        1 aaaaagacaa ggcttaaaaa ccttatcttt taaactatac cggcggccgg ggtcgaaccg
       61 gcacgtcctt gcggacactg gattttgagt ttggttcacg tgggtttata tgaataggaa
      121 ctcaggcaaa ccagcatttt atgaaattac ctctatcgta aaaacggtgg atttttttat
      181 ctttagtaac tttttaggaa ttaatatttt tttcgcttga attttgaaaa cctaaaatag
      241 attttgattt actaatttgt atcattacga tataatgatt ttaaggtact aaatgattta
      301 ggagggcaat catggaattt caagctagag tatctagtga gtgcatgtat tacattagtc
      361 tattagaaga aatttactcc aaagaaataa ctggagcagt tactagggga atagttctat
      421 caaaagcatt cgaagaaaca aaaaatctaa acaattggtt aaaaattagt gaggatacac
      481 atacaatacc cttacacaat atagaatatt caaaaggcta tggagtaaag attaaagctg
      541 aaatcaacga aaaaactgac cgaggaatta gaaatttaaa aattgagctt cctaaatatc
      601 taccagttcg gtctgtaaca attggcgtaa cagttaaact aatttgtaaa gcagcaatct
      661 tactaaggag agatgaaaag tttagacaaa ctgaaatatt atctgtttcc gaacattttg
      721 agcatttaga agaaaaatta aaaaaaatag ttgcaccagt taattacgat aaacttcaag
      781 atattttagc agatagtaaa aatgaaatcc aaaggaatgt taaataacat ttctttggat
      841 ttttttactt ctctaaacgt actctaaaaa tatattttta cacaaaaatc attgacaaag
      901 tatttttttc tgatatccta cctttgtaaa caattctagg atacattacc cttaataatg
      961 attggagagg tagtttgtca catgagtact gctcgagctt caccgataaa ataaaagtcg
     1021 tcccataccg tggaaaaata ccacatgaat acagaaagaa aaggaaataa attatgttcc
     1081 aaacactaat cgcattcatc tattctgcaa cagatgaaca atttcaagct ttccaaacaa
     1141 aggctaatgc cttgacagga aatgttacaa tctcccaaaa tgtggcacct attacagatg
     1201 ccttgaaaaa tcgttttggt cttaagacta tccaaactgc ttttgcacgc aagcttgtat
     1261 atgcttctac tcgccaccat tataaagacg gcaaaaccat gatggaggat attttagatg
     1321 gtaaaacacg tcgtcacgcc aatagctata tttaacacta aagggaggtg cacacctccc
     1381 tttatttgta ttatttagaa aatgaaagaa ggatagtgat atgactgtta ttggctttac
     1441 gcctaaccct aacgctcttg ttttgcaatt aacagagaca tcagttattg ctagacgaat
     1501 ttttttacca agagcaagtc cttactttga aattcttgaa agtaagccgt ttgactttgg
     1561 ctgggagaaa aatatcttag ttatttgtgc ccctaccact gcttcaaata atgcgtttga
     1621 actattattc ccttcaatgc taccacacgc aacaacggga gattttttca tattactttc
     1681 tatccttgat aaacaagatg gtgcaatcat tgcaggaact ctaggattaa aggactatgc
     1741 aacggttcgt aaaaatttga gagttaggag aaacaataag tatctgccaa tcatttggag
     1801 aggggactaa agcaatgtta aataacattt ctttggattt ttttaacctt ccgatagtac
     1861 cccaaaaata tttttttaca ctaaaatcac agaaaactct tgacaagata gacttagtat
     1921 gatattctat attaaatgat tttggggtac agtcccctag aaagggtttc ggaggtgact
     1981 ctatgtatga aattgaccaa gaagcgttag attatcttta tgatgaaatg ctttgcaatt
     2041 acgatgacga cgagcaggac gaagtctttg acggatacga ctgctaatca ttttttcaca
     2101 caggagcatt tgctccccaa attaacattt cagagaggac aaatttatgg atatatcaaa
     2161 atcagcacaa cttcaagagg caaacaatag attagaaact cttgtcaacg tgttcaactt
     2221 aaatccaaat ctgctaaaat attggaacga aggaaaattg tactactctt atctaacagg
     2281 tggaggtttg ctagcctcta ttgatacaat cacttatgac cctagatacc aagaacaggt
     2341 aaaacagtat gaggatagaa caggcaacct cgtatatcat gtaatagaaa gcggaagtct
     2401 gctcaatcta ctttatgtca gcgccccaga caaaaatatc tcggaagatg aacagcgtga
     2461 gatttgggat atggagctca tgaatcaaac ttttagctgg gtatctgcct atgtcatcaa
     2521 tctggacgac cctgacctat cagagcacgg cgatatctta cttggtgcct accaaggcgt
     2581 actaatcaga acggaataat tgcaggagga ggaattcctc ctcttgctga tttattaata
     2641 gaaaggttta ttattatgac acaacttacg atttccactt ggaatatcaa ccaacgctca
     2701 gggattggag aacaaattcc tgatatggtt gtaactgaac taagacaatt agatacagat
     2761 attatatgtc taacagagta tgtaaagact gaaaaccata atttattctg tactaaatta
     2821 caagatattg gctatgaaat ttttgaggat gcacgttctc ttggtttcgg aaatgaaatt
     2881 ctcattgcaa taaaaagtga tttagttaat gatagcaaag ttattactat tgacaatgat
     2941 gattgtaacc caaattttct acaagtcaga gtaactatta aaggaaaaaa attaaatatt
     3001 attggtgttc gaatcaagat aggtggaagt aatatcattg aagattttaa agaacgcaag
     3061 gtacaactag ataatctaat ttcaaatttg ccagcaagtc atgaagctac aatagttcta
     3121 ggtgacttaa ataatggctt cttcaaacct agtgatgatg tccattccta tcaaggaaaa
     3181 gctagagaat tttactctta tccattactt gtcaaagaaa tgagtgaatc aggtttaact
     3241 gcatatactc catcagatgt aaattcttgg aagtattgta aattagacca tatttttgct
     3301 aatgttccta tatcaaatga aacttattcc tggaaattca taaaaaatcc tgattataaa
     3361 agccaagtag gctacccaga ccatgcgatt ttatcagcaa ctgtttcttt ataattctcc
     3421 atgaggtgct gattatgatt aaaataaaga aagttgctga cttaaaaaga aattatacta
     3481 tttcaactag caaattaaag atagctcatt ggtcaatatt aggattctct acttgcatta
     3541 tgcttactat tattgcaaat attagagccc ctgaccttat taaaatccct ttatttattc
     3601 tagcaggatt tgttatatac aaatttcatc gtcaattaaa atattttaga tatcatttgg
     3661 attattacct aattatcaga gaaagtttat tgtatgtact atatactaac cgactttata
     3721 ctgctcaaaa agatagtaca gggtatgaaa aaattattag aagtgcaatc ttagaatatg
     3781 agctggatag gcaaaagggt cacgtactta tcaaagctct gattacaggc gatgaattta
     3841 gcaaaaaagt acagtcactt gatgatgtcc ttgctggagt tctcgaactt gaattagacg
     3901 agaaaattat ccgtcctagt tttgtagagt accactttta ctacaaaaaa ccagaaagat
     3961 taattctaca atctcacagc aaaaaacaaa tgattaacaa ccataatatt gatttaggat
     4021 acggaataat ctatgaccct gtaaaatgcc ctcacatttt ggtttcagga ggtacaggaa
     4081 gtggaaagtc agtttttatt actttcttga ttttagaact tctcaagcga caatcaactg
     4141 tctacatagc agacccaaaa aactctgacc tgggtagctt gtcacattac tttggagaaa
     4201 aatatgtagc aaccactcct aataatatcg ctcgtattgt ccgtttagta gtcgaagaaa
     4261 tgcaggaacg atatcaatat atgcgtgata actttctata cggctcaaac tttgcagacc
     4321 atggctttaa acctctatgg ctgatttttg atgaaatggg ggcatttcaa gcatcaggga
     4381 cagataaaaa atcaagagaa attattgccg aagttatgga cggaattaaa caaattattt
     4441 tactcggaag gcaatcaggt atatttatcc ttgtagcagc ccaacaaatg agagccgaaa
     4501 ccttaaatac tgaccttaga gataacttgg gattgagaat cgctcttggt gccaattcta
     4561 atgaaggtta tagaatggtc tttggctctg ctactcctga caatttgaaa cctattgaag
     4621 taaagggagc tggctatctt tatatgcagg ggtcaggcag ggaaaacgct caatactggg
     4681 aaagccccta cttagatacc aaacaattta attttataag tgaactacaa ttatatttag
     4741 aggaaaccaa ctaattgcaa tcgatatttt tactgaaatt cgtaagattt tcagtagaaa
     4801 tatcggttcc tcatcgctca gcgatgataa gaaaggaaac tattatgaaa ctaaatgatt
     4861 ttttgaaacc tgagctcctt ggtaatcgct tctttgccgt aaaaggatat actgaagtct
     4921 tagatagaga aacaaataaa ccaatagctt tacggctcaa tgtatcaatt caagatgaaa
     4981 cctcagattt ctttatggaa atgattcaag tcaaagtcaa cactttaacc cctactgctt
     5041 ctgttcaaga aatggcaaac aacaagactt gtcctgttgc gcttaaagat ttgaacgttg
     5101 gacaattcaa tggcaatctt tggtttgctt gctctgatgt cttacctgta acaaaatagt
     5161 tatgttaaaa tataacaaaa acatataaag gagttttaca atgcaaattt tcacttataa
     5221 cgattttcta aaagacaaag gaatcataac ttttgaacag gctgaaaaaa tttatgagga
     5281 tttaatcagc tcggtaaata ttcatgattc agaattcctt gaatactggc aaagactaat
     5341 tgagctatgt gctcaatatg ccgaggcacg tggtaagttc ttaactatac cattagccga
     5401 gaaggattct gctaataaat caaggacgcg gattcatgat tcaattatta ttcaactcaa
     5461 aattattaaa cgatacgctg aaaaaattgg aaatgatacc tcttggtttg ataagtttca
     5521 tgacgataga aaacgaatgg gggattttgc taattactta aattatgtct acgcagtaaa
     5581 tgcaagatag gagaaaccaa tgattgaaga tgtcaactat tattctcaac ctcctggaaa
     5641 atattccgat tcagaattac aagcaataaa agatttatta gaagtaatga tattttttat
     5701 tgatgatact cctcatacta ataataaacg aatttggttt gagccactca aatattggga
     5761 aaatacaaat tatataaggt tctcacacca aattgacttt cattctttac atcgtatcgc
     5821 tgaagaagat ttaagttggg aaggtggata taagttcaca ccacaacaaa ttgaagatat
     5881 attaaagtgg atgcaaactc aagaatggat aagaaatgag taagaaacca ttatagtagg
     5941 aggttttgtc aaaaattttt gccatgtccg atattcatgg acattatgat gaatttgttg
     6001 aaacctttca aaggaggatt tatatgatat aacaaccaat atattttggt tttagattat
     6061 atagatggca gttcacattc attaattgct aattacttat attatgatta tgcagtaaat
     6121 gcaagatagg aggtcaaatg aaaacattac tagtcggaga tttacatctg aaatctcaac
     6181 taattttgcc aatagttgac aatgttatca aagataataa tgtcaaaaga gttattttcc
     6241 tcggagatta tgttgacttt cctggtcaaa caaataatat aaatctctat gcacatgacc
     6301 tcacattttt atttagttgg aaaaagaata tggaagccaa tggtttggag gttatcaata
     6361 tcatcggtaa ccacgacgca gattatctta ttaatttcgt aagccagtat tctgtcgaaa
     6421 atatagaagt gttttttgct gttaaagaac aattaaagga actaggatta caagtagcat
     6481 ttcaattgaa cgattttctt atcagtcacg caggatatac cactcttttc aaccctgaac
     6541 cttggcactt tgaaaaaatc acctcaagac atcttcacaa actagaagaa ttggttgata
     6601 gcattggaac aaaacgagga ggactatttg aaactggttc tcctctgtgg gcagatttaa
     6661 gcgagctaaa agaaaaaaca aacttaaaat atcccaaaca gattgtaggt catactcccc
     6721 aaaaaaatat tgatacttct aataatatca ttggaattga tacattttca ttatacctta
     6781 ataaccacca tgaatatcaa gttatcggaa atggtgattt gattatccat gatgataagc
     6841 acaatgaatt aacagtcatc tcaacaaatt ggggaaatct agaaacatac gaaacattaa
     6901 aaaagttaat tcaataggaa ttttttatgt ctaaaattta tgccatgtcc gatattcatg
     6961 gacattatga tgaatttatt gaaagcctgt caaaagtgga tttatctgat aagaacaatc
     7021 aactaatttt acttggagat tatatagatg gcggttctca atcttttcaa gttatctcaa
     7081 aaatcattga actagaaatg aagtttcctg accaggtcat cactttatta ggaaatcatg
     7141 aagaatattt ttgtgagtgg ttatttgagg aagacggagc ttctaaatat acttcctatg
     7201 atactataat tagtttcatt ggtcagaata agcgaaaaaa tatcgttgat acagttattt
     7261 cagatttttc tctttctgat gaagaatgcc tagcccaaat taacaagaaa ataagagact
     7321 gtgttacttc tgaatatcct gaaatagtag attggtttag aaataaatat tttaattcag
     7381 aacgttttta tgagactgat actcaaatat ttgttcatgc tggaattgat gaagagttcg
     7441 gagaaaattg gaaattgtat tctaatcctg atatttttac taacaaattt ccagcaagta
     7501 tcggattttt ttataaggat attatttcag gacacattgc ttcttcagag gtcgcaaatg
     7561 acccaagcta tcttgggaaa atctactatg accaaaaaag tcattatttt attgatggtt
     7621 gtgttggcaa aagtcatatc atacccattc tttgttataa taccgtcact caatcatata
     7681 actactagga gggttaccta tgcaatattt tattgctgac actcatacat aactacttct
     7741 aatcatgtac ctaggtaaaa ctgccgactg taggtcggca agctggaagc gtgtttagcg
     7801 gagcggtgct taggcccaca tgatagaagt agggaggagc tttgctcctc ctagtttatt
     7861 tattatatag acaatctttt ggcgaaagcc aatcaaaaag gaactatttt ttatgacctc
     7921 atataataaa aaaatagtcc gcacttcttc ctatattgaa atttgggaat atgataagcc
     7981 aatattttca aaaggaaaat caattacaga tactaatact gaaaacaagg aaaaatcaaa
     8041 acgcagaaca tttgacgaac taacaccaga agaacaagaa caacgtcttg aacgcatgaa
     8101 aaaaactcgc ttagaagcaa agtggagctt actccgacta gtcgactgta actttgatga
     8161 tacgacttct tttcttacct tgaccactaa agaaaatatc agcgagagaa acgaattcaa
     8221 agacttactc aaaacattta tcaaacgctt taactatcac atatttaaaa ccaagaaaag
     8281 ccaattaaga tatatagcag tattagaaaa gcaaaaacgt ggagcatggc atgctcatat
     8341 tctacttttc agcgttccct atattccaca taagaaacta ttagagcttt ggggacacgg
     8401 agcagtacgg ataaataaag ttgatgttga tagcaaagaa aatcgtggcc gatatgttac
     8461 taagtatttt gaaaaaggaa ttggacaaga attgttggaa aatttcggta aacaagccta
     8521 tttatcatca cgtaatttga aaaagcctga tgaagataag ttttacacct atgaagtttt
     8581 caactatgat agttcagtcg tactatatga aacggaatac actagcaaag tttataaaga
     8641 cggtcagtat ttcgataatc ttgtaaagta taagaaaata aaattagatg attaaaggag
     8701 taagtcacat gatagcaact gtaaataaaa atgaccttgt tgcccttggt ttctctgaag
     8761 gaacttctaa acgtatcatc agacagggga aagagcttct aatagcaaga ggatttagag
     8821 tatatcaaaa taagcgtatt ggaactatcc ctgcttctat cgctactgaa ttacttggtt
     8881 ttgatgttca aaatagctct ctttctaggg ggtaatatat ggcaattaga aaaaataaag
     8941 acggtggttg gatagttgat attagcaatg gctttgaccc aataacacta aaacaacgta
     9001 gagtagttag aaagggctac aagaccaaaa aagaagctac cgaagcagaa cactacctaa
     9061 gaagtgtaga gctaaaagaa cgcttctatg gggctaaaat aactgtttct atgctctatg
     9121 aactgctaaa agaagaagat agcataaatt gccgcaagat tagctatatc aatactcaag
     9181 ataataacta caacagacat attaaagatt atttttcaaa ggttgataat gttggaaaat
     9241 tgacttatga ggatatatat cagtttaggg aacatcttaa acagaaagta gctcaaaatt
     9301 caaataaacc gttaaacaca aatactatca ataaaataat gatactactc aaaaaaatct
     9361 ttgacgtagg cttgcgaaaa ggttattacg ctacaaatcc tgtaaaacta ctaaaaaaat
     9421 taccaattga aaaaacaaaa atgcagtttt ggacagttga agaatttcag cagtttctaa
     9481 cgctgttaga gcctgaagaa tacaatatca aactactatt taccgttttg ttctttacag
     9541 ggttaagact tggagaagca ctagcactaa catggcaaga catcgacttc tcaagcaata
     9601 caattcatat cacaaaatct atctatgtca ataaaggaat tagccacatt agcacaacaa
     9661 agactaaagc aggaactagg aggattatta taaataaaaa attaagccaa gaactacagc
     9721 attggcaaca gcaacaaaaa cacctgttgg gacaatttac aagcgacagt ttaaccctcc
     9781 aagtgtttca aagtaaccct ctcacaatta cgaaaaatgc tattgagaag caatacaaga
     9841 aaatccttga acgcgatgac acactaaaaa agatacgcat acacgatttt aggcactcac
     9901 acgcttccct gctgattaac caaggggaag attatctagt tgtaaaagaa cggctaggac
     9961 acgcttcaat cacgactacg attgatactt actcccactt gtacccaagc aaacaaaaag
    10021 ctttggctga taaactggat gacctgctat aaaatcttga tatactaact aaaagaaaca
    10081 tcaagaaaat ctaaataata tgaaaggtat aaacaatgaa acataatttc gaagttaaaa
    10141 ttctaataaa agaaaacctt gacgaagacg ataaaactta tattcagaaa gtaataaaca
    10201 agttgaaaaa acgttacggc ataatacagg aaggaaatat acattataga aatgaagtag
    10261 agaaatttga tgattaccct tatagcgtga gttttgcttt aagactttca agatatacta
    10321 ggtactttga aacatttcaa tttaataata ttgcaattgg tgaatataat cgtatggaat
    10381 tcgagccaaa agtatggaaa gagagtcata atgcagaggt tgtaatattt ctaaaacaag
    10441 acttatctga agaagaagag aaatacatct taaatgaaat tgagaaatat aaaaaagaat
    10501 taggaatcat acaagacgga aatttatttt atagaaatga aagaaaatta ctagatgact
    10561 tccaattcat acttaaattc ggatacatga tgagtaaata taagaaatat tttgaagtct
    10621 tatatttaaa tagtatggca gaaggtatct acaatcatga gctttaaact aacaacgtgt
    10681 acatattgta atctatcaga ttaaccaagt agataaagat tagtaatttt tagtaactca
    10741 aacaggggaa actacaggca caaaacaaaa gacaaggctt agaaaccttg tcttttaaac
    10801 tataccggcg gccggggtcg aaccggcacg tccttgcgga cactggattt tgagtccagc
    10861 gcgtctgcca attccgccac gccggcatat agttgaactg gggtagctgg attcgaacca
    10921 acgcatgagg gagtcaaagt cccttgcctt accgcttggc gataccccaa aattaaaata
    10981 ggcgagtgag gggaatcgaa cccccgaatg tcagagccac aatctgatgt gttaaccact
    11041 tcaccacacc cgccatataa cacgggcagt aggaattgaa cccacactga aggttttgga
    11101 gaccttagtt ctacctttaa actatgcccg taaaggtatg gagagagagg gattcgaacc
    11161 cccgaacccg aaggagcgga tttacagtcc gccgcgttta acctcttcgc tatctctccg
    11221 aaatataata ttaaattaat ggcgcgagac ggaatcgaac cgccgacaca tggagcttca
    11281 atccattgct ctaccaactg agctaccgag ccaaattctc aaagaaattg cgggagcagg
    11341 atttgaacct acgaccttcg ggttatgagc ccgacgagct acctagctgc tccatcccgc
    11401 gataatagta aggaggatgt gggattcgaa cccacgcacg cttttacacg cctgacggtt
    11461 ttcaagaccg ttcccttcag ccggacttgg gtaatcctcc aaattatagt ccgtacggga
    11521 ttcgaacccg tgttaccgcc gtgaaaaggc ggtgtcttaa ccccttgacc aacggaccat
    11581 ataatgggca cgagtggaat cgaaccaccg acctcacgct tatcaggcgt gcgctctaac
    11641 catctgagct acgcgcccaa accaagtttg ggtatgaact atgttcaaag cgggtgacga
    11701 gaatcgaact cgcgacaaca gcttggaagg ctgtagtttt accactaaac tacacccgct
    11761 t
//
LOCUS       DATUCG010000079        11500 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.080, whole genome shotgun sequence.
ACCESSION   DATUCG010000079 DATUCG010000000
VERSION     DATUCG010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11500
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.080"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(25..2019)
                     /gene="pknB"
                     /locus_tag="VCA20_001619"
     CDS             complement(25..2019)
                     /gene="pknB"
                     /locus_tag="VCA20_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEP1835408.1"
                     /translation="MIQIGKIFAGRYRIVRQIGRGGMADVYLARDLILDGEEVAVKVL
                     RTNYQTDQIAIQRFQREARAMAELDHPNIVRISDIGEEDGQQYLAMEYVNGLDLKRYI
                     KENAPLSNDVAVRIMGQILLAMRMAHTRGIVHRDLKPQNVLLTSNGVAKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATIQSDIYAMGIILFEMLTGRIPYDGDSAV
                     TIALQHFQKPLPSVREENANVPQALENVVLKATAKKLSERYKSVAEMYADLASALSMD
                     RRNEPRVELEGNKVDTKTLPKLSQANVETKVPHTNSSAQVSATDKGSGKKEVAKSGNK
                     PVSKPRPGMRTRYKVLIGAILLTVIAAGLMIFNTPRTVTVPDVSGQTVEKATEMIEVA
                     GLEVGNITEEATATVDEGLVIRTSPAAKTTRRQGSKIDIVIATAALASIPDVVDKESD
                     TARQELEALGFQVTIKEEYSEKVAQGLVIKTDPEANSSAEKGAKITLYVSKGVTPQVV
                     PNVVGKTQENATQILQTAGFSIGTITQEYSSSVTAGQIISTDPVANTELAKGSIINLV
                     ISKGKELIMPDLTSRNYTYSQARSQLQALGVNAESIEKQEDRSYYSTTSDIVIGQYPA
                     AGATIDGTVTLYVSVASTRTSSDSSTGSSTSTSTSTDSGQ"
     gene            complement(2019..2756)
                     /locus_tag="VCA20_001620"
     CDS             complement(2019..2756)
                     /locus_tag="VCA20_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEP1835409.1"
                     /translation="MEIALLTDVGQKRSNNQDYINRYKNRAGIDLVVLADGMGGHRAG
                     HIASEMAATDLGAAWVDTQLVTLNDVREWMVAIIDAENQKIHELGQTEEYKGMGTTLE
                     AVVVIDNQMIYAHVGDSRVGLIRDGEYTRLTNDHSLVGALVRAGQLTEEEAQRHPQKN
                     IVTQSIGQAEPIEPDIALKTLEVGDYVLINSDGLTNMVPTEDIRDIVLSDVSIESKVE
                     TLVRFANNAGGLDNITVGLLHITEEAR"
     gene            complement(2794..4107)
                     /gene="rsmB"
                     /locus_tag="VCA20_001621"
     CDS             complement(2794..4107)
                     /gene="rsmB"
                     /locus_tag="VCA20_001621"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEP1835410.1"
                     /translation="MVINKHETARSLALSVLEQVFDQGAYSNIALNKALESSRLSAQD
                     KGLATELVYGTVSRKITLEWYLAHFIEDREKLDTWVYYLLMLSLYQLVYLDKLPTHAV
                     VNEAVNIAKRKPGTDKFVNAILRKLSQDSLPKPADIKRKNKRLSVQYSVPVWLVQTLI
                     AEYGDERAEKIFQSLHIRNKASVRVTDGQQVERLAEELGAQHSQLSPVGLVKSHGHFA
                     GTDCFKKGLITIQDETSQLVAPTLDIQGDEIILDACAAPGGKTCHMASYLTDGKVYAL
                     DLYDHKLNLIEENARRLGLADKIETKGLDASRVHETFGPDTFDKILVDAPCSGIGLIR
                     RKPDIRYNKASMDFDSLKTIQLQILDSVCQSLKIGGIITYSTCTIIAKENQEVIQEFL
                     QNHPNFEQVTLEHPQTDIMVDGCLLITPEQYKTDGFFIGQLRRKS"
     gene            complement(4094..5032)
                     /locus_tag="VCA20_001622"
     CDS             complement(4094..5032)
                     /locus_tag="VCA20_001622"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEP1835411.1"
                     /translation="MTKLIFMGTPAFSATVLRGLLADGNYNILAVVTQPDRAVGRKKV
                     IQMTPVKEVALEYNLPVYQPEKLSGSQEMAELMDLGADGIVTAAFGQFLPTKLLNSVD
                     FAVNVHASLLPKYRGGAPIHYALINGDERAGVTIMEMVKEMDAGDMISSDSIAIEESD
                     NVGTLFEKLAVVGRDLLLQTLPAYIAGDLKPVAQNPEQVTFSPNIQPEEEVLDWNKTA
                     RQLFNQIRGMYPWPVAHTYWQGERFKIQEAVEAEGEGPVGRVIVRSKKELIIATGQGA
                     LSLKTVQPAGKPKMTIADFLNGAGRDIAVGDQFGDQ"
     gene            complement(5164..7554)
                     /locus_tag="VCA20_001623"
     CDS             complement(5164..7554)
                     /locus_tag="VCA20_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEP1835412.1"
                     /translation="MLAQIIVDVPLMQTDQAYSYRVPAELEEGLKVGVRVHVPFGKGN
                     RLIQGIVVDLIDEDDENSLQDLKELAEVLDYSPVLNQEQFWLAEQMRKSVFSYKITLL
                     KSMLPSLLNSTYDKLLRPGLGLEMAEQISLFGDKEQIRFSDLDVTEQGKIMRLAQSGK
                     ILVDYVAKDKKQIKTEKWYRVCLEKLREADISNRAKKRQQLREFLLEQSEDQLLSNLK
                     SDYSADTLRYFQEKGYIEVWEEEVSRTQGVFDKVEKTQALDLNPEQAIAVREIVASIG
                     QESQTFLLQGVTGSGKTEVYLQVIDRVLKMGKTAIMLVPEISLTPQMTNRFISRFGQQ
                     VAILHSGLSDGEKYDEWRKIEAGNAQVVVGARSAIFAPLTNLGVIIIDEEHEATYKQD
                     SNPRYHARDVAKLRADYNRATLVLGSATPSLETRARASRGVYGRLTLNQRANPLARIP
                     EVEVVDFRDYIGQQEASNFTPVLIEKIREKLARKEQVVLMLNRRGYSSFVMCHDCGSV
                     DQCPNCDISLTLHMDTKTMNCHYCGFQKGIPQTCPNCQSRSIRYYGTGTQKAYDELQE
                     LLPEARILRMDVDTTKKKGAHEDLLERFGRGEADILLGTQMIAKGLDFPNVTLVGVLN
                     ADTALNLPDFRSSERTFQLLTQVAGRAGRADKEGEVLIQTYNPNHYAIAFAKEQDYEG
                     FYRYEMGIRKNLGYPPYYFTVGLTFSHKSEEFVVKKAYETVAFLRQHLTDAIQILGPT
                     PKPIARTHNLYHYQIILKYRHEDRLEEVLNQILDWTQERENQDLRLVIDNEPQNMM"
     gene            complement(7898..8212)
                     /locus_tag="VCA20_001624"
     CDS             complement(7898..8212)
                     /locus_tag="VCA20_001624"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEP1835413.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILEAKRAHELEAGAKPTQEFTSV
                     KSTLRALEEIESGNVVIHPDPEAKREAVRRRAEEAKRLAEEEERKIKAQIAKEKEEGE
                     KI"
     gene            complement(8236..8862)
                     /gene="gmk"
                     /locus_tag="VCA20_001625"
     CDS             complement(8236..8862)
                     /gene="gmk"
                     /locus_tag="VCA20_001625"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEP1835414.1"
                     /translation="MKERGLLIVFSGPSGVGKGTVRKEIFESSDNKFEYSVSMTTRPQ
                     RPGEVDGVDYFFRSREEFEDLIRQGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKKKVPDGVFIFLTPPDLEELQDRLIGRGTDSEEVIAQRIERAKEEIALM
                     REYDYAVVNDEVPLAAERVKRIIEAEHFRVDRVIGRYDEMIREQIAER"
     gene            complement(9010..10623)
                     /locus_tag="VCA20_001626"
     CDS             complement(9010..10623)
                     /locus_tag="VCA20_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009659039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEP1835415.1"
                     /translation="MNIITVVVTLVSALIGLVLGYFAISLKMKSAKETAELTLLNAEQ
                     EASNVRGRAEEQAEVILKTAERDRQTLKKELLLEAKEEARKYREEIAEEFKSERQELK
                     QIESRLTERATSLDRKDDNLTNKEKTLEQKEQSLTDKAKHIDEREQEVVKLEEQKALE
                     LERVAALSQEEAKEIILTDTRDKLTHEIATRIKEAEREVKERSDKMAKDLLAQAMQRI
                     SGEYVAEQTITSVHLPDDAMKGRIIGREGRNIRTFESLTGIDVIIDDTPEVVVLSGFD
                     PIRREIARMTMETLLQDGRIHPARIEELVEKHRVEMDNRIREYGEAAAYEVGAPNLHP
                     DLIKIMGRLHFRTSYGQNVLRHSIEVAKLAGVLAAELGENVNLARRAGFLHDVGKAID
                     REVDGSHVEIGTELAKKYKENPIVINAIASHHGDVEATSTIAVIVAAADALSAARPGS
                     RRESMEAYIKRLQDLEEIANSFEGIKQSYAIQAGREIRIIVHPNKVTDDQITILAHDV
                     REKIENNLDYPGNIKITVIRETRATDVAK"
     gene            10752..11234
                     /locus_tag="VCA20_001627"
     CDS             10752..11234
                     /locus_tag="VCA20_001627"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002885585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEP1835416.1"
                     /translation="MKKEVTVESFELDHTIVKAPYIRLISEEVGPKGDIITNFDIRLI
                     QPNENAMDTAGLHTIEHLLAKLIRQRIDGLIDCSPFGCRTGFHMIMWGKQDSEKIAQV
                     IKSSLEEIAEGITWEDVPGTTIESCGNYKDHSLHSAKEWAKLILSQGISTDAFERKPI
                     "
     gene            complement(11365..>11500)
                     /locus_tag="VCA20_001628"
     CDS             complement(11365..>11500)
                     /locus_tag="VCA20_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEP1835417.1"
                     /translation="EQGHRLLPLPPYSPEYNPIEKIWAHIKKHLRRVLPNCDTFLEVL
                     "
BASE COUNT         3104 a         2796 c         2164 g         3436 t
ORIGIN      
        1 gcatctgccc aaccttattc atctttattg tccgctatct gttgaagtac ttgtgcttgt
       61 ggaacttcct gttgaagaat cgctactggt tctggttgat gcaacggaga catataaggt
      121 aactgttcca tcaattgttg ctccagcagc aggatattgt ccaatgacga tgtcacttgt
      181 cgttgaataa tagcttctat cttcttgttt ttcaatactt tccgcattga cacccaaggc
      241 ttgtaattga ctacgcgcct gtgaataggt gtaattgcga gatgttaaat caggcataat
      301 caactctttt ccttttgaaa taactagatt gatgatagaa ccttttgcca actctgtatt
      361 ggcaacggga tctgtgctga taatctgacc tgcagtaaca gatgagctat attcctgagt
      421 aatagttcca atactgaagc ctgctgtctg aagaatttga gtagcattct cctgagtttt
      481 tccaacaacg ttcggaacaa cttgtggcgt cactcctttg gaaacgtaaa gggtaatttt
      541 tgcacccttc tcagcagaac tatttgcttc tggatctgtt ttgatgacga gtccttgggc
      601 aactttttcg ctatattctt cttttattgt cacttggaaa ccaagggcct ccaattcctg
      661 ccgtgcagta tcagactcct tatccaccac atctggaata gatgccagcg cagccgttgc
      721 tattacaata tcaatcttac tcccttgacg tctggtcgtc tttgcagccg gagaagttcg
      781 tattaccaat ccttcatcta cagttgcggt cgcttcttct gtgatatttc cgacttccaa
      841 tcctgctact tcaatcattt ctgtagcttt ttcaacggtt tgaccagaca catcagggac
      901 ggtaactgtc cgtggagtgt tgaaaatcat gagtccagcc gctatgactg ttaacaaaat
      961 agctcctatc aagactttat agcgtgtacg catacctggc cttggttttg aaacaggctt
     1021 atttcccgac ttcgccactt ctttcttacc agacccctta tccgtcgcag aaacttgggc
     1081 agaactgttt gtatgcggta ctttcgtttc aacattggct tgggagagtt tcggcaaggt
     1141 ttttgtatca accttgttcc cttctagttc aaccctgggt tcattccgac gatccatgga
     1201 tagagccgaa gctaaatctg catacatttc tgcaaccgac ttatagcgct cgcttaattt
     1261 tttagccgtt gcttttagta caacattttc taaggcttga ggtacgttgg cattctcttc
     1321 tctaacagat gggagaggtt tttggaaatg ctgaagagcg atcgtcacag cactatctcc
     1381 atcataggga atgcgacctg tcaacatttc aaaaaggatg atccccattg cataaatgtc
     1441 actttgaatg gtcgctttcg aaccacgcgc ctgctcaggt gaaagatagt gaacagaacc
     1501 caacatggag ttggtttgtg tcaaactcgt ttcagcaaag gcaactgcaa taccaaagtc
     1561 tgttacttta gcgactccat tggaagtcaa aaggacgttc tgaggtttca aatctcgatg
     1621 gacaattcct ctcgtgtgcg ccatgcgcat agcaaggaga atctgtccca taattcgcac
     1681 cgcaacatcg ttggagagtg gtgcattttc ttttatataa cgttttaaat caaggccatt
     1741 gacatattcc atagccagat actgttgacc atcttcttca ccgatgtccg aaatacgaac
     1801 gatattggga tggtcaagtt ctgccatggc acgcgcttca cgttgaaaac gctggatagc
     1861 aatttgatct gtctgatagt tggtccgaag aactttaaca gcaacctctt ctccatctaa
     1921 aatcaaatct cttgctaggt aaacatctgc cataccgcct cgaccaattt gccgaacgat
     1981 tcgataccga ccggcaaaga tcttaccgat ttgaatcatt acctagcctc ctccgtaatg
     2041 tgaagcaaac ctactgtaat attatctaga ccgcctgcat tattggcaaa acgtacaagt
     2101 gtttctacct tgctttcaat tgaaacatca ctcaatacaa tatcacgaat gtcctcggtt
     2161 ggtaccatat tggttaagcc atcgctgtta ataaggacat aatctccaac ctcaagagtt
     2221 ttcaaagcaa tgtctggttc aattggttcg gcttgtccaa tggactgcgt tacaatattt
     2281 ttttgaggat gacgctgtgc ttcttcttct gtgagctgac cagctcgaac aagggcacct
     2341 accagcgaat ggtcgttggt tagacgtgta tattcgccat ctctaatcaa tccaactcgt
     2401 gaatctccaa catgggcata gatcatttgg ttgtcgatga caacaactgc ttcaagagtt
     2461 gttcccatcc ccttgtattc ttctgtctgt ccaagttcat gaatcttttg gttttcagca
     2521 tcaatgattg caaccatcca ttcacggaca tcatttaagg tcactaattg agtatcgacc
     2581 caagcagctc ccaaatctgt tgctgccatt tcactagcaa tatggccggc acggtgacca
     2641 cccattccat cagctaacac gaccaaatca atccctgcac ggtttttgta gcgattgata
     2701 tagtcttggt tgttcgaacg tttctgtcct acatcagtaa gtaatgcaat ttccataatt
     2761 tttatcgttg ctaagcaact tcctcctctt attctaggat tttcgtctaa gttgaccgat
     2821 gaaaaagcca tctgtcttat actgttcagg ggttatcaac aagcaaccat ccaccataat
     2881 atctgtttga gggtgttcta gtgtcacctg ttcaaaattg ggatggtttt gcagaaattc
     2941 ttgaataacc tcttggtttt ccttagcaat aatcgtacat gtgctatagg ttattatacc
     3001 accaattttc aagctttggc aaacactgtc cagtatctgc aactggatag tctttagaga
     3061 atcaaaatcc atcgatgcct tgttataacg aatatctggc ttacggcgga tgagtccaat
     3121 acctgaacaa ggggcgtcta ccaatatctt atcaaaggta tcaggtccaa atgtttcatg
     3181 aacacgactg gcgtccagac ctttggtctc aatcttatct gctagaccta aacgacgtgc
     3241 gttttcttca atcaggttta gtttatgatc atataaatcc aacgcataaa cttttccatc
     3301 cgtaagatag cttgccatat gacaggtctt tccacccgga gccgcacagg catccaagat
     3361 gatttcatct ccttgaatat ccaaagtcgg agctaccaat tgactggttt catcttgaat
     3421 ggtgataagt cctttcttga aacaatctgt ccccgcaaag tgtccatgag atttaaccaa
     3481 cccaactggt gataactgcg agtgttgggc gcctagctcc tctgccaatc gctccacttg
     3541 ttgaccatct gtcacacgta cactggcctt gttccgaata tgtaagcttt ggaaaatctt
     3601 ctctgcacgt tcatcaccat attctgcaat cagggtttgg accaaccaaa ctggcacaga
     3661 atactgaacg gatagacgtt tgttctttct tttaatatct gctggtttcg gcaaggaatc
     3721 ctgactaagc ttacgcaaaa tggcattaac aaatttgtca gttccaggct ttcgcttggc
     3781 aatattaact gcctcattga caactgcgtg ggtaggcaat ttgtccaaat ataccaactg
     3841 gtagaggctc aacatgagga gatagtagac ccaagtgtcc aacttttccc tatcttcgat
     3901 aaaatgcgcc aaataccact ctagggtaat tttacgagaa accgtcccat aaaccaattc
     3961 ggtcgccaag cccttgtcct gtgctgataa acgagaagat tctagagcct tattgagagc
     4021 gatgttcgag taagcacctt gatcaaagac ttgttccaat acagataagg ctagacttct
     4081 tgctgtttca tgtttattga tcaccaaatt gatctcctac tgcaatatct cgacctgcac
     4141 catttaaaaa gtctgcaatg gtcatttttg gcttaccagc tggctggaca gttttcagag
     4201 aaagggctcc ctgacctgtc gcaatgatca attctttctt gcttcgaaca ataacccgac
     4261 caactggtcc ttcaccttct gcttctacag cttcttgaat tttaaaacgt tccccttgcc
     4321 aataggtgtg ggcaacgggc cacgggtaca taccgcgaat ctgattaaat agttgacgag
     4381 ctgtcttatt ccaatccagt acctcttctt ctggctggat atttggtgag aaggttacct
     4441 gttctggatt ttgagcgact ggtttcaagt caccagcaat ataggctggt aaggtttgta
     4501 ataataaatc tcgaccaaca acagccaact tttcaaacaa ggtgccgaca ttgtcgcttt
     4561 cttcaatagc aatgctatcg ctggaaatca tgtcaccggc atccatttcc ttgaccattt
     4621 ccatgatggt tacaccggca cgttcatcac cgttaatcaa agcgtaatgg ataggggcac
     4681 caccacggta tttaggaaga agagaagcat gaacattgac tgcaaagtca actgagttca
     4741 gtaatttagt cggtaggaat tgtccaaaag ctgccgtcac aatcccatct gcacccaagt
     4801 ccatcagctc agccatttct tgacttcctg ataacttttc cggttgatag actggtaaat
     4861 tgtattccaa ggctacttct ttcacaggtg tcatttggat gacttttttt cgtccaacag
     4921 ctcggtctgg ctgggttaca actgccagta tgttgtaatt tccatcagct aagagccctc
     4981 ttaagaccgt cgctgaaaac gctggcgttc ccataaaaat caactttgtc atattcgtgt
     5041 tctcatctcc ttttcatttt ctgattcata tcaattatac tatctcctaa ttatatcatt
     5101 tttgcttatt ttttccttaa tcaaaggttc ttaaaacaaa aaaatgcaag gtcaatagtg
     5161 acctcacatc atattctgcg gttcattatc aatcaccagc cgcaaatctt gattttctct
     5221 ctcctgcgtc caatccaaaa tctgattcaa tacctcttcc aaacggtctt catgccggta
     5281 tttcaagatg atctggtagt ggtagagatt atgggttctg gcaataggct tgggcgtcgg
     5341 tcccaagatt tgaattgcat ctgttaagtg ttgacggaga aaggctacag tttcataggc
     5401 ttttttcacg acaaattctt ctgacttgtg cgaaaaggtt agacccaccg taaaatagta
     5461 aggtggataa ccaagatttt tccgaatgcc catctcataa cgatagaaac cttcataatc
     5521 ctgctctttg gcaaaagcaa tggcataatg attggggtta taggtctgaa tcaggacctc
     5581 cccttcctta tcagctcgac cagcccgacc agctacctga gttagcaact ggaaggtccg
     5641 ctcagaagac cgaaaatcag gcaaattcag agccgtatcc gcattgagca cgccaactag
     5701 ggtcacattt ggaaaatcca agcccttggc aatcatctga gttcccagaa gaatatccgc
     5761 ttcgcctcga ccaaagcgtt cgagcaagtc ctcatgggca ccttttttct tggtagtatc
     5821 cacatccata cgcaaaattc tagcttctgg caagagctcc tgcaattcat cgtaggcctt
     5881 ttgcgtaccc gtcccataat aacgaataga acgactttgg cagtttggac aggtctgagg
     5941 aatccccttt tgaaaaccac aataatggca attcatcgtt tttgtgtcca tgtgaagtgt
     6001 aagggaaata tcacaattgg gacactggtc aacgctaccg caatcatgac acatgacaaa
     6061 agaagaataa ccacgcctat tcaacataag aacgacctgt tccttacgag ccaatttttc
     6121 tcttatcttt tcaatcagaa caggggtaaa attactagcc tcctgctgac ctatataatc
     6181 acggaaatcc acaacttcca cttctggaat acgagccaag ggattggccc tctggttaag
     6241 agtcagtctt ccatagactc ctcgactagc ccgagcccgt gtttcaagac tcggcgtagc
     6301 agaccccaag accagggtcg ctcgattata atccgcccgc aactttgcca catcacgcgc
     6361 atggtagcgt ggattgctgt cctgcttgta ggtcgcctca tgctcctcat caatgatgat
     6421 aacacccaag tttgtcaaag gggcaaaaat agccgaacgg gccccgacaa ccacctgggc
     6481 attccctgcc tcaatctttc tccactcgtc atacttctct ccatcagaca gaccggagtg
     6541 aagaatcgcg acctgctgac caaaacgaga aataaaacga ttggtcatct gaggggtcaa
     6601 ggaaatttca ggcaccagca taatagccgt tttccccatt ttcaagaccc tatcaataac
     6661 ctggagatag acttccgtct tcccagaacc tgtcactcct tgcagtagaa atgtctgact
     6721 ttcctgaccg atagaggcca caatctcccg aaccgcaatg gcctgctctg gattcaaatc
     6781 caaggcctgc gttttttcta ccttatcaaa aacaccctga gtccgcgaca cttcctcttc
     6841 ccagacttct atatagcctt tttcttggaa atagcggagc gtatcagctg aatagtccga
     6901 ttttaggttt gaaaggagct ggtcttccga ctgttccaga agaaattccc ttaactgctg
     6961 ccgtttttta gcccgattgc tgatgtctgc ttctcgtaat ttctccagac aaactcggta
     7021 ccatttttcc gtcttgattt gcttcttatc cttggccaca tagtctacca agatttttcc
     7081 tgactgggcc agacgcatga ttttcccttg ctctgtcacg tccaagtcag aaaaacgaat
     7141 ctgctcctta tcaccaaaca gactaatctg ctctgccatt tccaaaccaa gccctggacg
     7201 gagcaatttg tcataggtcg aattgagcaa gctaggcaac atggatttta agagggtaat
     7261 tttataggag aagaccgact ttctcatctg ctccgccaac caaaattgct cttgattgag
     7321 aacaggacta tagtccaaaa cctcggcaag ctctttcaag tcttgtaagc tattttcatc
     7381 atcttcatca atcaaatcaa ctacaatacc ttgaatcagc cgatttccct taccaaaggg
     7441 tacatggaca cgcacgccaa ccttgagccc ctcttccaac tcagctggaa ctcgatagga
     7501 ataagcttga tctgtctgca tcaaagggac gtctacaata atctgtgcta acataccatc
     7561 tccatttatt ttaccttcta atatcacgac tgatgcgaac aaggttcact ttatgtataa
     7621 cgaggctggg caaaaagccc aggaccacta ctcagagttc gtgtcaacat ctcagcgcag
     7681 tggttgattg gcatatttgt tcgttttttg cactccaaat ctagcctaat caactgtgcg
     7741 ggggcgggaa gacgaactct ttttatctcg gtcgagttct ttcccactcc ccaaatatta
     7801 aaagataaac taaacgacct gcctcctttc tgtcaaaact ttcatcattc cgaaagtgaa
     7861 aagaaacccg gatggcaatc ccaaccgagt cttgtttcta aatcttttcg ccttcttctt
     7921 tttctttagc aatttgagct ttgattttac gctcttcttc ttccgccaaa cgtttagcct
     7981 cttcggcacg gcgacgaaca gcctcacgtt tcgcttctgg gtctgggtgg atgaccacat
     8041 tacctgattc aatttcttcc aaggcgcgta gggtggattt cactgaggtg aattcttgag
     8101 ttggtttggc acctgcttcc aattcatggg cacgcttagc ctcaagaata accaaagaat
     8161 acttagatgg taccttatcc aacaaggtat caattgacgg cttcaacatc ataatgcgat
     8221 tctctcttct ttcttttatc gttctgctat ctgttcacga atcatttcat cgtagcgacc
     8281 aatcactcgg tctacacgga aatgttctgc ttcgataata cgcttgacac gctcagcagc
     8341 caacggaact tcatcgttca caactgcata atcatactca cgcatcaagg caatctcttc
     8401 cttggcacgt tcgatacgtt gagcaatcac ttcctcacta tccgttccgc gaccaatcaa
     8461 gcgatcttgc aactcctcca agtcaggtgg tgtcaagaaa atgaacaccc catctggaac
     8521 ctttttcttc acttggaggg caccctgtac ttcaatttct aaaaagacat caattccctt
     8581 atcaagagtt tcattgacat aggtcagagg tgttccatag taatttccaa cgtactctgc
     8641 atattccaac atttgaccct ggcgaatcaa atcttcaaat tcctcacgac tacggaagaa
     8701 atagtcaact ccatcaactt ctcccggtct ctgtggacgt gtcgtcatgg atacagaata
     8761 ttcaaattta ttatcagagc tttcaaaaat ttcctttcga actgttcctt ttccaacacc
     8821 agatgggcca gaaaagacaa tgagtaagcc tcgctctttc atgatatctc cttttaaatc
     8881 tttcattcta gtgtaacaaa atttagcatg aatttcaact gcttttaaat acgattagaa
     8941 tattttctat tcccaaccca aaacgaactt tgagccagta gaaaaagagc ccttcggact
     9001 cttctacatt tacttagcaa catctgttgc tcttgtttca cggataaccg tgattttgat
     9061 atttcctgga taatccagat tattttcaat tttctcacga acatcatggg ccaagatggt
     9121 aatttgatca tcagtgacct tgttaggatg aacgataatc cgaatttcac gaccagcctg
     9181 aatagcatag gattgtttaa tcccttcaaa actgttggcg atttcttcca agtcttgcag
     9241 acgtttgatg taggcttcca tagattcacg acgcgaacct ggacgagcag cgctcagagc
     9301 atctgccgca gccacaatga ctgcaatagt acttgtcgct tctacatccc catggtgact
     9361 agcaatcgca ttgataacaa ttggattctc tttatatttc ttagccaatt ctgtaccgat
     9421 ttcaacgtgg cttccatcca cctcacggtc aatagcttta ccaacgtcat gtaggaaacc
     9481 tgctcgccgt gccaaattca cattctcgcc caattcagca gcaagtacac ctgctaattt
     9541 agcaacttcg attgagtggc gaagaacatt ttgtccataa gaggtacgga aatgcaaacg
     9601 tcccatgatt ttaatcaagt ctggatgaag gtttggtgcc ccaacttcat aagcagcagc
     9661 ttccccatac tcacgaatac ggttatccat ctctacacgg tgtttctcaa ccaactcctc
     9721 aatacgagct ggatgaatac gaccatcttg aagcaaggtc tccatggtca tacgggcaat
     9781 ctcacggcga atcggatcaa aacctgatag aacaaccact tctggtgtat catcaatgat
     9841 aacatcgata cctgtgagac tttcaaaggt acgaatattt cgaccctcac gtccaataat
     9901 ccgtcccttc atcgcatcat ccggtaaatg aaccgatgta atcgtctgct cagccacata
     9961 ttcacctgat atgcgttgca tcgcttgggc taacaagtcc tttgccatct tgtcagaacg
    10021 ttcttttact tcacgctctg cttccttgat acgagtcgca atttcatgcg tcagtttatc
    10081 acgtgtatcg gtaaggatga tttccttcgc ctcttcttga ctgagggccg ccactcgttc
    10141 tagctccaaa gctttttgtt cttcaagttt tacaacttct tgttcacgct catcaatgtg
    10201 cttagcttta tcggtcagac tttgttcctt ttgttccaaa gtcttttcct tgttggtcaa
    10261 attatcgtct ttgcggtcca aactggtcgc acgctctgtc aagcgtgatt cgatttgctt
    10321 caactcttga cgttctgact taaattcttc tgcaatttct tctcggtact tacgagcttc
    10381 ttccttagct tccaagagta attctttttt caaggtctgg cgatcgcgct cagctgtttt
    10441 tagaataacc tcagcttgct cttctgctcg accacgaaca ttactagctt cttgttctgc
    10501 gtttaaaagt gtcaattctg cagtttcttt tgcagatttc atcttcaaag aaatggcaaa
    10561 gtaacctaat actaaaccaa tgagagcaga aacaagtgtc actactactg taatgatatt
    10621 catatttcta cctctttttt ataattcgat tttcacaaaa tctgtatcta ttttatcata
    10681 tttgaaacga tttcacaatt ccaaattgtc aaatatggta taataaattc atcacttatt
    10741 tggaggaaac catgaaaaaa gaagtcactg tcgaaagctt cgagctagac cacactattg
    10801 taaaagcacc ttacatccgc ttaatttctg aagaagtcgg accaaaagga gacattatta
    10861 caaactttga tattcgcctt atccaaccca acgaaaatgc aatggataca gctggtctac
    10921 ataccatcga gcatctatta gctaaactaa ttcgccagcg catcgatggg ctaatcgact
    10981 gctctccttt cgggtgtcgg acaggtttcc acatgattat gtggggaaaa caagactctg
    11041 aaaagattgc tcaagtcatc aagtcgagtt tggaagaaat tgcagaaggc atcacttggg
    11101 aggatgttcc tgggacaacc attgaatcat gcggtaacta caaagaccac agccttcatt
    11161 cagctaaaga atgggccaaa ctaatccttt ctcagggaat ctctaccgat gcctttgaaa
    11221 gaaaaccaat ctaacaaaaa aacggataca ccgctcagaa cgtagacaaa atcctaaaat
    11281 ataaaaattg aagaagtttg cgaaaaatag caagtcatta aactcataga aacattgata
    11341 tagtcaactg aaacaggagc aggactaaag tacctcaaga aacgtatcgc aattcggcaa
    11401 tactcttctg aggtgttttt tgatgtgagc ccatattttc tcaatgggat tatattctgg
    11461 tgagtaagga ggaagtggta aaagtctatg tccctgctcc
//
LOCUS       DATUCG010000080        11490 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.081, whole genome shotgun sequence.
ACCESSION   DATUCG010000080 DATUCG010000000
VERSION     DATUCG010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11490
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.081"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            64..1674
                     /locus_tag="VCA20_001629"
     CDS             64..1674
                     /locus_tag="VCA20_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEP1835418.1"
                     /translation="MPKDFNELPRQNEKNEALDYVKALIDQARIDGRNEDVVQLDKII
                     KLLNRKKYGLVWEEHAELVEEEMKTRIPVFIEDETRKIRANPEDKDYNFLLEGDNLHS
                     LHLLEKTHAGRIDVIYIDPPYNRGKTDFKYNDLYVDKLDSFRHSKWLSFMSARLHLAR
                     GLLKKEGLIFISIDDYEFSQLKLLLDSIFGEENFVNTFVWKRNSSGKTEKDKFTVNTE
                     YVLLYSKTRDFVLSPAYKPLSDATIKSYNKDDKDGRGRYASISLQKPKDPGPETTYDY
                     VDNSGKVWNCPAKGWRMTYDKVKALENDNRLITSGKTLRVKDYWNERASEGKRIDTLW
                     SDLPENTTGSKELDKVLREKGKFDNPKPTKLIERCLQISTKDALILDFFAGSGTTGHA
                     VAQLNKEDGGNRKYILCTNNENNICEEVTYKRLTNIQDDLPHNLKYFKTKFLSKDDEE
                     LEFSLLHHVHTLIELEHGIDLKESDKATAFSLSELRKLDLSGIKTVYVRQQSHAMMEK
                     SDLVRFEGIELIDVPEYYFAKEMREAGL"
     gene            1674..3929
                     /locus_tag="VCA20_001630"
     CDS             1674..3929
                     /locus_tag="VCA20_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEP1835419.1"
                     /translation="MDLFPFQEQAVQTLQDKFFYSQKQTTVFYAPTGAGKTVMLIKLM
                     DRIIEHNPYPYDYVFVWLTPGNGELEEQSWSKAKDYATFVKAQTLDDALISGFEKNTA
                     TFLNWERVKNSQALALRDGETKNLTALIKNARLQNLHFVLIIDEEHRNQTEKAQPIID
                     MFDADKIIRASATPKSSSSEYDNVQVNDEDVIAQGLIVRNVELNPDGVDGDVVDNMVQ
                     YFLDLADDKRRKIKHAYKELGLDINPLVLIQFPDEKKANKEERNNLISQVREYLAEIG
                     QDDSQIATWLANEKINVTSIEKANSPVNYLLMKQAVSTGWDAPRAKILVKLRLNTEPN
                     FTLQTIGRIRRMPQQKHYDNPILDNAFIYSNDQSYIADVLRQGEGSFIATYELQEGTP
                     DFQLMSVKPNTRTGLTNAEIVAALRSQFKKDYGLVEQNGHQNQEKLESFGYRFGTNIR
                     QKLIQSDKLERDILSDKLRSYQVGVTVNLKDNRLDLLNAQQKLQSHLYRDTASDVNVI
                     LVELFSNRSEHPQKRFLSLKPSEFMAFVINNYHLIRNTVKNADVHGLFDKQLSLEYGI
                     SHDEVDLVPFILPKYEMYRVIKGKSGRVFTKNVYKGYGENNWTNSKAPEIAFEKWLEE
                     SSEVSWWYRSKDRGEKYFSVAYGHKAEGFFPDYIFKGRDGITYIVETKGGNKQNIDKY
                     SEAKFKALREWSENSKTNPHGAKFAFVRPIKNSIGEVIGMLFNNTSWKEDMSDRNHWK
                     PISEFFKDDLF"
     gene            complement(4039..4818)
                     /locus_tag="VCA20_001631"
     CDS             complement(4039..4818)
                     /locus_tag="VCA20_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1835420.1"
                     /translation="MIISMKNVHYKRQGKAILEDINWEFKQGERWAILGLNGAGKSTL
                     LRILMVEFWKTSGDLTVLGVEFGKGDIPSLRTKIGVVGSFLTERFPIDLTAEQIVLTG
                     KYKSSILYREYGQAELDEAIDMLKTIKAEHLNGRTYASLSQGERQLLLIARSLMEEPA
                     ILILDEATVGLDLLARERLLHHIDHICQLPTAPAIIYVTHHVEEITENFTHVLLLKDG
                     MVLAQGPKEDILTPELLSDFYGNQVELIDLGEGRLFIKPIL"
     gene            4951..6288
                     /locus_tag="VCA20_001632"
     CDS             4951..6288
                     /locus_tag="VCA20_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEP1835421.1"
                     /translation="MEDPGSQTIYLQLLILFLLTLFNAFFSASEMALVSLNRSRVEQK
                     AADGEKKYIRLLQVLENPNNFLSTIQVGITFISILSGASLANDLGAVFAKWMGNSTTA
                     QTAGYWLALAMLTFVSIVLGELYPKRIAMNMKENLAVVTAPVIIFLGKIVSPFVWLLS
                     AATNLISRITPMNFDDADEQMTRDEIEYILTKSEKTLDAEEIEMLQGVFSLDELMARE
                     VMVPRTDAFMVDIEDDTATIMAAILKQNFSRIPVYDGDKDNIIGLIHTKKILSEAFSN
                     GFDNLNIRRIMQEPLFVPETIFVDDLLTSLRNTQNQMAILLDEYGGVAGLVTLEDLLE
                     EIVGEIDDETDKTEIFVREIADNTYIVQGNMTLNDFNEHFEMELESDNVDTIAGYYLT
                     GVGTIPSQDEKVSFEVDSKDHHLVLINDKVKNGRVTKLKLLITPLEKEETDKD"
     gene            6409..7200
                     /gene="pflA"
                     /locus_tag="VCA20_001633"
     CDS             6409..7200
                     /gene="pflA"
                     /locus_tag="VCA20_001633"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEP1835422.1"
                     /translation="MMTEIDYRKVTGLVHSTESFGSVDGPGVRFVVFMQGCHMRCQYC
                     HNPDTWDLVNPAATERTAEDVLNEALRFRMFWGKEGGITVSGGEATIQIDFLIALFTL
                     AKEKGIHTTLDTCALTFRKTERYLEKYSRLMEVTDLVLLDIKEMNPEQHRIVTGHSNK
                     TILECAQYLSDIGKPVWIRHVLVPNLTDRDEDLIELGKFVKTLKNVERFEVLPYHNLG
                     EFKWRELGRPYPLEGTKPPTKARVENAKALMETETYQEYLNRIKG"
     gene            complement(7310..9007)
                     /locus_tag="VCA20_001634"
     CDS             complement(7310..9007)
                     /locus_tag="VCA20_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEP1835423.1"
                     /translation="MKFSDYTYVRPDYEAIKAQFSDLTDQLSQASDLETTRTLVAAVT
                     KLLNLVDTQYNLWMIRHTIDMNDEFYNEETKFWNEYSPLFEELTTNYYRVIVQTPYKE
                     ELSDILPKTFFMQAENKLKTFSSDVIPLFQKENELTDEYSKLIAGAEIGFQGQTYNLA
                     QMGPFCQSTDREVRKAASAATTAFFESKEADFDRVYDELVKVRTEIAHKLGFKDYVEY
                     GYLKMNRFDYNRDMVKVYREEILKHIVPIVQNLRERQAKRLQVPSLKHYDLNLEFLDG
                     NAVPQGDPDFIVSQAQDMYRELSVETGEFFDFMIEHELLDLVAKPGKNSGGYCTYIPD
                     FKSPFIFSNFNGTSGDIDVLTHEAGHAFQVYRSRWIQSPEVVWPTYETCEIHSMSMEF
                     MTWPWMDRFFKEQVDKYKFTHLASALLFLPYGVLVDHFQHEVYEHPEMTPGERKATWK
                     KLQDLYLPDRDYSESEALNRGIFWYRQGHIFASPFYYIDYTLAQVCALQFWKRTQVDH
                     DENAWEDYIRICDLGGTKSFLQVVEAANLQSPFKEGALESTAKAAADWLNTVKDSDL"
     gene            9145..10080
                     /locus_tag="VCA20_001635"
     CDS             9145..10080
                     /locus_tag="VCA20_001635"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEP1835424.1"
                     /translation="MSKFLVFGHQNPDTDAIASSYGWAHLEREVFGRDAEAVALGTPN
                     EETAFALDYFGVTAPRVVESAKAEGVSQVILTDHNEFQQSIADIKDVEVAAVIDHHRV
                     ANFETANPLYMRLEPVGSASSIVYRAFKENGVTPPKEVAGLLLSGLISDTLLLKSPTT
                     HATDPQVAAELAEIAGVNLEEYGLALLKAGTNLASKSAEELIDIDAKTFGLNGNDVRV
                     AQVNTVDIAEVLERQAEIEAAMTAASATNGYSDFVLMITDIVNSNSEILALGSNVDKV
                     EAAFNFKLENNHAFLAGAVSRKKQVVPQLTDAFNA"
     gene            10292..10756
                     /locus_tag="VCA20_001636"
     CDS             10292..10756
                     /locus_tag="VCA20_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003104139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEP1835425.1"
                     /translation="MQSAINFKEVETTCLETSPVAEVLAGLRANEARYFWNKYKQEFV
                     VYTPEEKPEILPFIKKVLAERDMVFPYNPLNVAQLEVDGILWSFVFYDNGLAVNVLYT
                     LEEGGKRVVEFKLSDGIEIPKEFEGKFKVACQRSKLAGEIRGTFFVVKGEYL"
     gene            10980..11390
                     /locus_tag="VCA20_001637"
     CDS             10980..11390
                     /locus_tag="VCA20_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEP1835426.1"
                     /translation="MNIKEVSDVTGLSADTIRYYERIGLIPKIARKSSGVRKFSESDV
                     AILEFIRCFRSAGMSIERLIEYMDLVQAGDSTVEARIDLLKEEQEELRSRLSEIQQAL
                     DRLDYKIENYQTILRGAENQLFTDGGSSFKKGRG"
BASE COUNT         3546 a         2078 c         2564 g         3302 t
ORIGIN      
        1 caactacttt ttttcagaat actctaaaac acctgcaatc atagagattg ggggtgtttt
       61 ctaatgccta aagactttaa tgaactccct cgtcaaaacg agaaaaatga agctttggat
      121 tatgtgaagg ctcttattga ccaggccaga attgatggtc gaaatgaaga tgtagttcag
      181 ctcgataaaa ttataaaact tcttaatcgt aagaaatatg gattagtttg ggaagaacac
      241 gctgaattgg tcgaggaaga aatgaaaaca aggattcctg ttttcattga agacgagacc
      301 cgaaaaataa gagcaaatcc agaggataag gattacaatt ttctcttaga gggggataat
      361 ttacacagtt tgcatctatt agagaaaact catgcgggta gaatagatgt aatttatatc
      421 gacccaccgt ataatagggg aaagactgat tttaaatata atgatttata tgtagataag
      481 ttagattcgt ttagacatag taaatggctt tcatttatgt ctgctcgctt gcatttggca
      541 cgtggtttgc taaaaaaaga agggcttatt tttatctcaa ttgatgatta tgaattttct
      601 caactgaaat tgttattaga ttctattttt ggtgaggaga attttgtaaa tacttttgtt
      661 tggaagagga attctagcgg taaaacagaa aaagacaaat ttacagttaa tactgagtat
      721 gttcttctgt attcaaaaac tagagatttt gtgttaagcc ccgcatataa accattatct
      781 gacgcaacaa taaagtcgta taacaaagat gataaagacg gaaggggaag atatgcaagt
      841 attagtctgc aaaaaccaaa agatcctggt cctgaaacta cttatgatta tgttgataat
      901 tctggaaaag tatggaactg tcctgctaaa ggttggcgaa tgacatacga taaggtgaaa
      961 gctttagaaa atgataatag acttattaca tcaggaaaaa ccttgcgtgt aaaagattat
     1021 tggaatgaaa gagcatcgga agggaaaaga attgatactt tatggtcaga tttgccagaa
     1081 aacactactg ggagtaaaga actagataaa gtgttacgag agaaaggtaa gtttgataat
     1141 ccaaaaccta caaaactaat tgaaagatgt ctacaaatca gtacaaaaga tgcactcatt
     1201 ctcgacttct tcgctggttc gggtactacg ggtcatgcag ttgctcaatt aaataaggaa
     1261 gatggtggga atcgcaaata tattctttgt accaataatg agaataatat ttgtgaggag
     1321 gtaacttaca aacgattgac aaatattcaa gatgacttgc ctcataatct aaaatatttc
     1381 aaaacgaaat tcctttccaa agatgacgag gagctagaat tcagtcttct acatcatgtc
     1441 cataccctta ttgagttgga acatggtatt gatttgaaag agagtgataa agcaacagca
     1501 ttttcacttt cagaattgag gaaacttgat ttgagtggta tcaaaacggt ctatgttcgt
     1561 cagcaatccc atgccatgat ggaaaagtca gatttagttc gtttcgaagg catcgaattg
     1621 attgatgtac cagaatatta ttttgctaaa gaaatgagag aggcaggttt atgatagatc
     1681 tatttccttt tcaagagcag gcagtccaga ccctgcaaga taaatttttc tattcccaaa
     1741 aacaaactac agttttctat gcaccgactg gagcaggaaa aacagtaatg ctcattaagc
     1801 tcatggatcg aattatagag cacaacccct atccttatga ttatgttttt gtatggttga
     1861 cacctggtaa tggagagttg gaggagcaat cttggtccaa ggctaaagac tatgccactt
     1921 ttgttaaagc gcagacatta gatgacgcat tgatttcagg gtttgaaaaa aatacagcaa
     1981 cttttttaaa ttgggagagg gttaagaata gccaagcgct tgccttgcgg gatggagaga
     2041 caaaaaatct tacagccctt atcaagaatg caagattaca aaatttgcat ttcgttctga
     2101 ttattgatga ggaacacaga aatcaaacgg agaaggcaca accgataatt gacatgtttg
     2161 atgcggacaa aatcatacgt gcttctgcaa caccgaaatc atcttccagt gaatacgata
     2221 atgtacaagt caatgacgag gatgtgattg cacaggggct aattgtccga aatgttgaac
     2281 tcaatcctga tggtgttgat ggggatgttg ttgacaatat ggttcagtac tttttagact
     2341 tggctgatga taagcgtagg aaaatcaaac atgcttataa agagctagga ctagatatca
     2401 atcccctggt tttgattcaa tttcctgatg agaagaaagc aaataaagag gagcgtaata
     2461 atctaatttc gcaagtacga gaatatttag cagaaattgg tcaagatgac agtcagatag
     2521 ctacgtggct ggcaaatgaa aaaatcaatg ttacttcaat agaaaaagca aatagtccag
     2581 ttaactactt attgatgaag caggcagttt caactggttg ggatgctcca agagcaaaga
     2641 tcttagttaa actgagatta aataccgaac ctaattttac attgcagacc attggtcgca
     2701 ttcgtcgtat gccccagcag aagcattacg acaatcctat cttagataat gcctttatct
     2761 actccaacga tcaatcctac attgctgatg ttttgagaca aggggagggg agttttattg
     2821 cgacttatga attacaagag ggtactccag attttcaatt gatgtcagtt aagcctaaca
     2881 ctaggacagg attgacgaat gctgagattg ttgcagccct tagaagtcaa tttaagaaag
     2941 actacggttt ggtggagcaa aacggtcatc agaatcagga aaaacttgag agttttggtt
     3001 atcgatttgg aacaaatatt aggcaaaaac tcattcaatc ggataagtta gaaagagata
     3061 ttctatctga caaacttcga agttatcaag taggcgttac agttaatctc aaagataata
     3121 gacttgattt actcaatgca cagcaaaaac ttcagtctca tttatatcga gatacagcta
     3181 gtgatgtcaa cgtgattcta gttgagcttt tttctaaccg aagtgagcat cctcagaaaa
     3241 gatttttaag cctcaaacct tcagaattta tggcatttgt cattaacaat tatcatttaa
     3301 tcaggaatac agtaaagaat gcagatgtac atgggttgtt tgataaacaa ctatccttgg
     3361 agtatggtat cagtcatgac gaagtcgatt tggttccgtt tattctaccc aaatatgaaa
     3421 tgtaccgagt tataaaaggg aaatcaggga gagttttcac gaaaaatgtc tataaaggtt
     3481 atggcgaaaa caactggact aatagtaaag ctcctgaaat tgcatttgaa aaatggctcg
     3541 aagaatcgtc ggaagtttct tggtggtatc gcagtaagga tagaggagaa aagtattttt
     3601 cagttgcata cggtcacaaa gcagaaggat tttttccaga ttatattttc aaagggagag
     3661 atgggattac atatattgta gaaacaaaag gtggcaataa acaaaatatt gataaatatt
     3721 cagaagcaaa atttaaagcc ttgagagaat ggtctgaaaa ctccaaaacg aatccccatg
     3781 gagctaaatt tgcttttgtt cgtcctatta agaatagcat tggagaagta ataggcatgc
     3841 tattcaacaa tacaagttgg aaagaagata tgtcggatcg caatcattgg aaacccatta
     3901 gcgaattctt taaagatgat ttgttttagc gaatctacta tgtctgtttg acttattgaa
     3961 attattcgtg acttatgaca cctgacaaaa atacctctta ccaatttatc tggttagagg
     4021 tattttattc aacttcatct ataatatcgg cttaatgaac aatcttccct cacccaagtc
     4081 aatcaattct acctgatttc cataaaaatc gctgaggagc tcaggtgtca ggatatcttc
     4141 tttgggtccc tgtgccaaga ccatgccatc tttgagcagg aggacatggg tgaaattttc
     4201 agtgatttct tcaacgtgat gggtgacgta gataatggcg ggagccgttg ggagttggca
     4261 gatatggtcg atgtggtgga gcaggcgttc gcgagccaaa aggtctaagc ccacagtagc
     4321 ctcgtccaaa atcaaaatag ccggctcttc catcaaactt cgggcaatca gtaggagttg
     4381 gcgttcgccc tgtgacaaac tagcataggt ccgaccgttc aagtgctcag ccttaatggt
     4441 tttgagcatg tctatggctt catccaattc agcttgaccg tattcacgat agaggatgga
     4501 ggatttgtac tttccagtta ggacaatctg ctcagctgtc aaatcaatgg gaaagcgttc
     4561 ggtaaggaaa gaaccaacga ccccaatctt agtgcgtagg ctaggaatat ctcccttacc
     4621 aaactccact ccaagaacag tcaggtcacc agaggtcttc caaaattcaa ccattaaaat
     4681 gcgaagtaga gttgactttc cagctccatt gagccctaaa atagcccaac gctctccttg
     4741 tttaaattcc cagttgatgt cttctaaaat agctttacct tgtcgtttat agtggacatt
     4801 cttcatcgaa ataatcatag ttgctctcct attccttttg attattgtaa cataaacaga
     4861 catgagggac taattataac aagaaatatg ttaaatttta gaatttgtgg tataatggaa
     4921 atgttataaa ttttattata ggagaacatc atggaagacc ctggtagtca gaccatctat
     4981 ttacaactgt taattttatt cttattgact ctgttcaatg ccttcttttc agcctctgaa
     5041 atggcgcttg tttctctaaa tcgttcaaga gtagaacaaa aggccgcaga cggtgagaag
     5101 aaatacattc gcttgcttca agttttagag aatcctaaca attttctatc aaccattcag
     5161 gtcggtatta ccttcatctc cattctttca ggtgccagct tggctaatga tttgggagct
     5221 gtatttgcca aatggatggg gaattcaaca acagcgcaaa ccgcaggata ctggcttgca
     5281 ctggctatgt tgaccttcgt ttcaatcgtt cttggcgaat tgtaccctaa gcggattgcc
     5341 atgaatatga aggaaaatct ggcagtagtc actgcaccag tgattatttt ccttgggaaa
     5401 attgttagtc cgttcgtgtg gctattatca gctgcaacga atcttatcag ccgtattact
     5461 cctatgaatt ttgatgatgc ggatgagcaa atgacacggg atgaaattga atatattttg
     5521 acaaagagtg agaaaacctt agatgccgaa gaaatcgaaa tgcttcaagg tgtgttctct
     5581 ttagatgagc tgatggcacg tgaagtaatg gttccccgta cggatgcttt catggtggat
     5641 attgaggatg atacagcaac catcatggca gctatcctca agcagaattt ttcacgtatc
     5701 cctgtttatg atggcgataa ggacaatatc attggtctga ttcataccaa gaaaatcttg
     5761 tcagaagctt tcagtaatgg ctttgacaat ctcaatatcc gccgcattat gcaggagcct
     5821 ctcttcgttc cagaaacgat tttcgtggat gatttgttga catcgttgag aaatacccaa
     5881 aatcaaatgg ccatcttgtt agatgagtat ggtggagttg caggtctcgt taccttggaa
     5941 gatttgctgg aagaaattgt cggggaaatc gatgatgaga ccgataagac agaaattttt
     6001 gttcgagaaa ttgctgacaa cacctatatc gtccaaggga atatgacctt gaatgacttc
     6061 aatgagcatt ttgagatgga gttggaaagt gataacgttg atacgattgc tggttattat
     6121 ctgacaggag tcggtaccat tccgagccaa gacgagaaag tttcctttga agtagatagt
     6181 aaagatcacc acctggttct tattaacgat aaggtaaaaa atggtcgcgt aacaaaatta
     6241 aaactcttaa ttacaccact ggagaaagaa gaaaccgata aggactaggg caacctagtc
     6301 ttttagcttt cttcttatgg aagatgatag ttatgaaaga aagaaagcaa atgatagaag
     6361 cgttttcatt tttgtgatat actagtctta taaaatgatg aggatgagat gatgacagaa
     6421 attgattatc gtaaagtgac aggtttggtt cattcaacag agagttttgg ttcggttgat
     6481 ggcccagggg tacgttttgt agtctttatg cagggctgtc acatgcgttg ccagtattgc
     6541 cataacccag acacttggga tctggtcaat ccagcagcga ctgagagaac ggcagaagat
     6601 gtcttgaatg aagctctgcg ttttcgtatg ttttggggaa aagaaggggg tattactgtt
     6661 tcaggtggtg aagcaaccat ccagattgat tttttaattg ctctgtttac ccttgcaaaa
     6721 gaaaaaggga ttcacactac tttggatacc tgtgcgttga ctttccgtaa gacagagcgt
     6781 tacttggaaa aatatagtcg gcttatggaa gtaacagatt tggtgctcct tgatattaag
     6841 gaaatgaatc ctgagcaaca ccgcattgtg acaggtcata gtaacaagac tattttagaa
     6901 tgtgctcaat atctatcaga cattggtaag cctgtctgga ttcgtcatgt cttggtgcct
     6961 aacctgacag atcgagatga ggatttgatt gagcttggca aatttgtcaa gactctcaag
     7021 aatgtagagc gttttgaagt cctgccttat cataacttgg gagaatttaa gtggcgtgag
     7081 ctgggcagac cctatccact agaggggact aagcccccaa cgaaggctcg agtggaaaat
     7141 gcaaaggcct tgatggagac agagacctat caggagtact tgaatagaat taagggataa
     7201 tagtgaagag gtcggagatt gatgcgaaaa agatttaatg tgttagggac aagtatcata
     7261 taatcaaaaa atctgaatga gtcatttgat tcattcagat ttttgtgttt tataagtcgc
     7321 tatccttaac tgtatttaac caatcagcag cagctttagc tgtgctttca agggcacctt
     7381 ctttgaacgg tgattggagg tttgcggctt caaccacttg caagaaggac ttggtgccgc
     7441 ccaaatcaca gatgcggata tagtcttccc aagcattttc atcgtgatct acctgtgtac
     7501 gtttccagaa ttgaagggca caaacttggg ccaaggtata gtcgatgtag tagaatggac
     7561 tagcaaagat atgaccttga cgataccaga agatgccgcg gttgagggct tctgactcag
     7621 aataatcacg gtctggcaag tagaggtctt ggagtttttt ccaagtcgcc ttgcgttcac
     7681 ctggtgtcat ctctggatgt tcatagactt cgtgctggaa gtgatccacc aagacaccgt
     7741 aaggcaagaa gagaagggca cttgccaagt gggtaaactt gtacttatcc acctgttcct
     7801 tgaagaaacg gtccatccat ggccaggtca taaattccat agacatagag tggatttcac
     7861 aggtttcata ggttggccag acaacttctg gactttgaat ccaacgcgaa cgatagactt
     7921 ggaaagcgtg acctgcttcg tgggtcaaga catcaatatc accgctggtt ccgttgaagt
     7981 tggagaagat gaatgggctc ttgaagtcag ggatataggt acagtagcca ccgctgtttt
     8041 tgcctggttt tgcgaccagg tccaagagct catgctcaat catgaaatcg aagaactcgc
     8101 ctgtttctac ggacaattca cggtacatgt cttgggcctg gctgacgata aaatcagggt
     8161 caccttgtgg caccgcattg ccgtctaaaa actcaagatt gaggtcgtag tgtttcaggc
     8221 ttggtacctg caagcgtttg gcttggcgtt cacgaagatt ttgcacaatc ggtacgatat
     8281 gcttgaggat ttcctcacgg taaaccttga ccatatcacg attgtaatcg aaacggttca
     8341 tcttgaggta gccgtattcc acatagtcct tgaagccaag tttgtgggcg atttccgtgc
     8401 ggactttgac caattcatcg taaacacggt caaagtcagc ttccttactt tcaaagaagg
     8461 cagttgtggc agcagaagca gccttgcgga cctcgcggtc ggttgattga cagaatggcc
     8521 ccatttgtgc caagttgtaa gtctgtcctt ggaagccaat ctctgcacct gcaatcagtt
     8581 tgctgtactc atcagtcaat tcattttcct tttggaacaa gggaatcaca tcagatgaga
     8641 aggtcttgag cttgttttct gcctgcatga agaaggtttt aggcaagatg tctgacaact
     8701 cttccttgta cggcgtctgg acaatgacac ggtagtagtt ggtggtcaat tcttcaaaaa
     8761 gtggagaata ttcattccaa aacttggttt cttcattgta gaactcatcg ttcatgtcaa
     8821 tagtatgacg gatcatccag aggttgtact gggtatcgac caggttgagt aattttgtaa
     8881 cggctgccac aagtgtgcga gttgtttcca agtcacttgc ctgagacaac tggtctgtca
     8941 agtcagaaaa ctgagccttg atagcttcat aatcgggacg gacataggta tagtctgaaa
     9001 atttcatatc tttctccttt tgtattgcta cctactatct tactcttttt gaaaacgttt
     9061 ggcaaatccc gacccgtcca tgttcacaag tctttctatt tatggtaaaa tgtaggagat
     9121 tattagaaaa atgaggttgt agacatgtct aaatttttag tttttggtca ccaaaatcct
     9181 gatacagatg ccattgcatc atcgtacggc tgggctcact tggagcgtga agtgtttggt
     9241 cgtgatgcgg aagcagttgc acttggaacg ccaaatgaag aaacagcgtt tgcgcttgac
     9301 tattttggtg ttactgcacc gcgcgtggtt gagtctgcta aggcagaagg cgttagccaa
     9361 gtcatcttga cagaccacaa tgaattccaa caatcaatcg cagacatcaa ggatgtggaa
     9421 gtggcagctg ttattgacca ccaccgtgtc gcaaactttg aaacggcaaa tccattgtac
     9481 atgcgtttgg aaccagtagg ttcagcatca tcaatcgttt accgtgcctt taaagaaaac
     9541 ggagtaacac ctccaaaaga agtagctgga cttctcctat caggtttgat ttcagatact
     9601 ctcttgctca aatcgccaac cactcatgca acagatccac aagtagcagc tgaattggct
     9661 gaaattgctg gtgtgaactt ggaagaatac ggcttggctt tgttgaaagc tggaaccaac
     9721 cttgccagca agtcagcaga agaattgatt gacatcgacg caaaaacctt tggtttgaac
     9781 ggaaatgacg tgcgtgtagc tcaagtcaac acagtggaca ttgcagaagt cttggaacgc
     9841 caagcagaaa tcgaagcagc tatgacagca gcgtcagcaa caaatggcta ctctgacttt
     9901 gtcttgatga ttacagacat cgttaactca aactcagaaa tccttgcgct cggtagcaac
     9961 gtggacaagg tagaagcagc cttcaacttc aagttggaaa acaaccacgc cttccttgca
    10021 ggtgccgttt cacgtaagaa acaagtggtg ccacagttga ctgacgcttt caacgcctaa
    10081 tgaatttttc tacctacctc gaacctgaag taggtaggaa ggccttatct agcaccttcg
    10141 ggtgcttttt tactaaagga gaagctatgt cttatacagt atagtctagt gaattagaag
    10201 caggacatat ataagtttgt caatttgaga actctaaagt tattttagga gtgggttatt
    10261 aacactataa cttaaaattg tctttttcct aattcaatcc gctataaatt ttaaagaagt
    10321 cgaaacgaca tgcttagaaa ctagtccagt cgcagaagtt ttggcaggtc tgcgtgccaa
    10381 tgaagcccgc tatttctgga acaaatacaa gcaggagttc gtggtctata caccagaaga
    10441 gaagcctgaa atcctgccct tcatcaagaa ggtcttggca gagcgggaca tggtttttcc
    10501 ttacaatccg ctcaatgtcg ctcagcttga ggtagatggc attctctggt catttgtctt
    10561 ttatgacaat ggcctggctg tcaatgtcct ctatacgctt gaagaaggag gcaaacgagt
    10621 tgtcgaattc aaattgtcag acggcattga aattcccaaa gaatttgaag gtaaattcaa
    10681 ggttgcctgc caacgctcca aactagctgg cgaaattcgt ggcaccttct ttgttgtcaa
    10741 gggcgagtat ttataggatg gcaaatctgg ggaaacccag atttttatat agtcaagtga
    10801 attagaaacg ggacatatct aagtttgtca atttgagaac tctaaagtta ttttaggagt
    10861 gggttattaa cactataact taaatctggc cttttcctaa ttcaagtcgc tatataatga
    10921 cttgacctgg agtggacttc aaggtgtata ataagcgaaa acaaagaaag gagactggaa
    10981 tgaatatcaa agaagttagt gatgtaacag gtctatcagc agataccatt cgctactatg
    11041 agcggatagg acttattccc aaaattgcaa gaaaatcttc tggagtaagg aagttttcgg
    11101 aaagtgatgt agccattttg gaatttatcc gctgttttag gagtgctggt atgtctattg
    11161 agcgcttgat tgagtatatg gacttggtgc aggcaggaga tagtacagtg gaagctcgga
    11221 ttgatttact aaaagaggaa caggaagaac tgcggtcacg cttgtcggaa attcagcaag
    11281 ctctagatcg tttagattat aaaatagaaa attaccaaac tatattaaga ggtgcagaga
    11341 atcaattgtt cacagacggc ggtagttcat tcaaaaaagg cagaggataa ctgctctgcc
    11401 tttataatta tgtgactttt agtccgtcac gccccgtaag gtgcgtgaca aataaaccac
    11461 ccgctatgcg ggtgcgcgac gaaggttata
//
LOCUS       DATUCG010000081        11119 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.083, whole genome shotgun sequence.
ACCESSION   DATUCG010000081 DATUCG010000000
VERSION     DATUCG010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11119
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.083"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            <1..306
                     /locus_tag="VCA20_001638"
     CDS             <1..306
                     /locus_tag="VCA20_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEP1835427.1"
                     /translation="APKKDSEAPKSDKVETDKPMPETKQPDMKQPKADKPEAEKAQMP
                     RTEGMKPESKASMMPKAEAPKATLPNTGEASSAIGWLGGALATLATGLYLFKNKKEE"
     gene            741..1457
                     /locus_tag="VCA20_001639"
                     /pseudo
     CDS             741..1457
                     /locus_tag="VCA20_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921854.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
     gene            1605..2795
                     /locus_tag="VCA20_001640"
     CDS             1605..2795
                     /locus_tag="VCA20_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEP1835428.1"
                     /translation="MQTFPVLAVVKNFKERIFFSRDEITSAAGLRLKVSESAKPNNEG
                     ITIVGSVLGPDSSPESDRTPEPRGDRTPDIDVPKAEVPKVEQPKPKAPEVQPPAPKSP
                     ETVPPKVMPPAPKVPDVKTPKVEYPDTDAPEIESPKAPETERPEVLPPTPKVPEVEVP
                     KVIPPTSKVPEVATPKATPLTPKSPEAETPKVEQPKPKVPEVHLPAPKTPETVPPKAT
                     PPAPKVPDVKTPKEAPLSSKTPGVEVPKVTPPTSKVPEAETPKVEQPKPKVPEVHLPA
                     PKSPEVEVPKVTPPTSKNPDVKTPKSPEVAHPEVEKPAGIQPEKGQPEVNKPNIIHSE
                     VLQSEVKKTESGVSMAPKVGIQKETLPNTGETSNTLVWLGGALATLASSLYLFKQHRQ
                     REEE"
     gene            complement(3394..4652)
                     /locus_tag="VCA20_001641"
                     /pseudo
     CDS             complement(3394..4652)
                     /locus_tag="VCA20_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013904617.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(4745..4930)
                     /locus_tag="VCA20_001642"
     CDS             complement(4745..4930)
                     /locus_tag="VCA20_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004049124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEP1835429.1"
                     /translation="MYAAQYLLPILTQKELLQQLQISPNTLKSWEKKGLSRLEPPIEN
                     TRTVFYKISDVIDFLSK"
     gene            5016..5321
                     /locus_tag="VCA20_001643"
                     /pseudo
     CDS             5016..5321
                     /locus_tag="VCA20_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261835.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
     gene            complement(5415..6068)
                     /locus_tag="VCA20_001644"
     CDS             complement(5415..6068)
                     /locus_tag="VCA20_001644"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019947.1,HMM:NF024705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEP1835430.1"
                     /translation="MTDEELLNIYIQLLPFLQQVLGSNTEIVIHDIRKPDCSLIAIEN
                     PVSGRTVGAPLTDMFRQMIANKEYENKDFISNYNGSSKSANFLSSTFFIKNQKKLIGL
                     LCINKDTNSVSELNLAIKSLLQGYNLSLPEDSTYSETFESNAMDEIIKERIQNTILNT
                     RTLPKRMSVDEKIMVIKTIQQEGVLSMKGAVAETANQLHISVPTLYRYIKRASQDNK"
     gene            6266..6661
                     /locus_tag="VCA20_001645"
     CDS             6266..6661
                     /locus_tag="VCA20_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEP1835431.1"
                     /translation="MNIAIKTNLVPEAIGPYSTAIETKGNCQKTVYVSGQLPIDVATG
                     EFAGSDIKEQTTQSLKNLRGILNAADMDLSDVVKTTVFLSDITEFAAMNEVYGEFFAD
                     PYPARSAFQVAALPKNARVEIEAIAVKSN"
     gene            6678..7871
                     /locus_tag="VCA20_001646"
     CDS             6678..7871
                     /locus_tag="VCA20_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEP1835432.1"
                     /translation="MGKYNFDEEISRYGSSCIKYDYGMQRMGRTDLLPMWVADMDFKL
                     PEEILDVFRKRIDHGIFGYSDPDEDYWQAMNHWFGTRHGFTIDPETVTLGAGIVYGLA
                     TAVKAYSEPGEAVLIQQPVYYPFRDVIEKNGRKFVNSQLIYDDGHYSIDFEDFEQKII
                     DNNVKVFILCNPHNPVGRVWTKEELTQLADICLRHNVVILCDEIHCDFVYPGHKTTNF
                     MTLDKKYHDSLVLFLSPSKTFNVAGFQPANIVIPNPELRSKYRASNDSAAYSNASLMG
                     MLAVKACYTLGGDWVDEMVDYLYGNMVYMRDFVKDNFPKATFVEPEGTYLIWVDFSGY
                     GFSDEELQHIMVEEAKLWLDSGKIFGPATAQFERFNIACPRSTVVKAMAQLKEAMDKH
                     EKWEQ"
     gene            7886..9100
                     /locus_tag="VCA20_001647"
     CDS             7886..9100
                     /locus_tag="VCA20_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HEP1835433.1"
                     /translation="MKNIWNTYKSSFILLASMVIGGAIGLFWGPDAAILQPVADTFLH
                     LLYCCVVPLIFCSLSAAIARMEDLTKLKKILGVFLAGTFISGIISCLFMLIPSMIFDP
                     AAGSTVSLTETVEDASGSMNILGMFTVGDFPELFSRSNLMALIVFTVIFAFALIQVGE
                     KAHGIVKGLENMTEVIVKIIGIVMKIAPLGLGCYFAILLGTNGSDVIAPLSKAILMYM
                     VVIIIYFIVSQTAYAYIGAGKEGVKRWWATAVKPTLTALGTCSSAATLPVNIEQAKQI
                     GIPDEITNLVVPLGANLHKDGACVIQILKIAFLCSVFNIEFVTPRNILIAIAVSVIVS
                     VVMGGIPGGGYVAEIFIMSAFGFPAVAIPIMVLIGTITDAPATAVNVTGDTGLAMVVT
                     RFVEGKDWLKKS"
     gene            9546..10385
                     /locus_tag="VCA20_001648"
     CDS             9546..10385
                     /locus_tag="VCA20_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEP1835434.1"
                     /translation="MKKLKNIKPRWFVFICILIFLALGFDSRLQVTHYRLVSPKISQP
                     IRLALITDLHSSNYGKNQIQILEVLEKEGPDIILLGGDIFDDELSDRKTLELLSGLSK
                     FNHTYYVLGNHEIWSNRYEQMLEHLRQQNVAILSGKSSRVDVRGQSITISGIDDPEYF
                     QKYDTSESLLDQLEDIKGKQISTDFQVLLAHRPELIDTYQKYGFDLVLSGHAHGGQWR
                     VPYLVNGVFAPNQGFLPKYAGGLYEVENSHFIVSRGLAKESTRVPRFFNRPEIIIVDL
                     VNQ"
     gene            complement(10579..10728)
                     /gene="rpmG"
                     /locus_tag="VCA20_001649"
     CDS             complement(10579..10728)
                     /gene="rpmG"
                     /locus_tag="VCA20_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEP1835435.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVI
                     FTEVK"
     gene            complement(10744..10926)
                     /gene="rpmF"
                     /locus_tag="VCA20_001650"
     CDS             complement(10744..10926)
                     /gene="rpmF"
                     /locus_tag="VCA20_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEP1835436.1"
                     /translation="MAVPARRTSKAKKNKRRTHYKVAAPTVKFDETTGDYSRSHRVSL
                     KGYYKGRKIAKAASAE"
BASE COUNT         3540 a         1937 c         2147 g         3495 t
ORIGIN      
        1 gctcctaaga aagattcgga agcaccaaaa tctgataaag tagaaactga caaaccaatg
       61 ccagaaacta aacaaccaga tatgaagcaa ccgaaggcag ataaaccaga agcagagaaa
      121 gctcaaatgc cacggacaga gggtatgaag cctgaatcta aggcttcaat gatgccaaaa
      181 gcagaggcac caaaagcaac tttgccaaat acaggcgaag caagtagcgc aataggctgg
      241 ctaggtggtg ctttagccac tcttgccaca ggcttgtatc tattcaaaaa caaaaaagaa
      301 gaatagtcat tattgaataa gatatgggaa cttccctctt ctaagttcat aaaagactcc
      361 agatcatcgc tgtagaagcc ttggtttgga gtctttttgt taaatattct tagaaaataa
      421 caaaatagat aaatattaga taccctctag aaaaaattta gtttaggatg ttatactaac
      481 gctaaaacaa taatatcaat ttttgaaaat actagattgg aataaaaatc taatcgccat
      541 attttagaac ctgtacttac aagtaaagag cttttatgta agagatagtt tttcgctaat
      601 caagttaatt ttttgaggga atgctggctg tattttgaaa aaacgaacga tgagtagctt
      661 aaaaatgcct tagatgggag tgctaacctg taaatacagg ttcttagtaa attgaagtaa
      721 atagagtaaa ggagcacatt atgagcaaac aaaaagttgt gacaaatcta ttgcttagta
      781 cagctgtact aggtggtttt ctaatatgtc acgtaccatc tgtaagcgca gaaacaggca
      841 atcttcaaga aatacctact gaatctgcta cggttagctc agaaaatcaa actgcgaccg
      901 cagtcgatgg aatttcacct acggataacc aattacctac gtcagaagaa ggtgatgaac
      961 aggctccaga agaaaaacta gtttctagcg attctacgag agaagaaagt gctacgcagg
     1021 tagcgacaga tccggataca ccatctcaat cattaaatgt gtcagaggcg gaaaattcaa
     1081 cggcagattc aaatattcaa caagccgaag agaacctgat tcaagaatcg aattatcata
     1141 gatttgacac tcatcaaggt gaacagatta ggaatgcagt atctatagaa aaaatatcgg
     1201 cagataatga caagattaag tggagagtaa cttttgatag aaaaaattgg acctttagtg
     1261 gagaggggag tggctattac tttattcttc caaaaggctt acaattaacc aatattattg
     1321 ataaacagtc tgaagaggat attttcaata agttccctaa ggatgtcaat agtgaggcaa
     1381 attctggcgg gcagccgtat cgctttttca gtagagaaaa aaatgctaat aatgatgagc
     1441 gtagttttga aagtcagtgg ggttggtctg ttggacgtgt aggaggagag ctagaaaaat
     1501 ggaaggaaga aagttccagc atctattttc tacaagtaca gcgagattcc gttgctaaag
     1561 aaaaagcgac atatgaactg acggcacagg tgacaaatcg agaaatgcag actttcccgg
     1621 ttttagctgt ggtgaagaat tttaaagagc gaatattttt ctccagagat gagattactt
     1681 ctgctgctgg tttgagatta aaggtatcgg aaagtgctaa gccaaataat gaagggatta
     1741 ctattgtcgg atctgtccta ggacctgatt catctccaga aagcgacagg actcctgaac
     1801 cgcggggaga taggactccg gatatagatg taccaaaggc agaggttcca aaagttgagc
     1861 aaccaaaacc aaaggcccct gaagttcagc cgcctgctcc taagtctccg gaaactgtgc
     1921 caccaaaagt aatgcctcca gcacccaaag ttccagacgt taagacgcca aaagttgagt
     1981 accctgatac agatgctcct gagattgagt caccaaaagc tcctgaaact gaacgaccag
     2041 aagtattacc accgacccca aaagtcccag aagttgaggt tccgaaagta attccaccga
     2101 cctcaaaagt tccagaagtt gctaccccaa aagcaacgcc actgacgcca aaatccccag
     2161 aagctgagac accgaaagtt gagcaaccaa aaccgaaggt tccagaagtt caccttcctg
     2221 ctcctaagac tccagaaact gtgccaccaa aagcaacgcc cccagcaccc aaagttccag
     2281 atgttaagac gccaaaagag gcgcccctgt catcaaaaac tccgggcgtt gaggttccga
     2341 aagtaacacc accgacctcg aaagttccag aagctgagac accgaaagtt gagcagccaa
     2401 aaccgaaggt tccagaagtt caccttcctg ctcctaagtc tccagaagtt gaggttccga
     2461 aagtaacacc accaacctca aaaaatccag acgttaagac gccaaaatct ccggaagttg
     2521 cgcacccaga agttgagaaa cccgccggaa tccaaccaga aaagggtcaa cctgaggtta
     2581 ataaacccaa tattatccat tcggaggtgc ttcaatccga agttaagaaa acggaatctg
     2641 gagtttcaat ggctccaaag gtaggaatac aaaaagagac tttaccaaat acgggtgaaa
     2701 cgagcaacac cctagtatgg ctaggtggtg ctctagctac ccttgcatca agtttgtatc
     2761 ttttcaaaca acacaggcaa agagaagagg aatgatttta cgctaatact atggtttttg
     2821 gaacaccaat aaggcttatt taccatggac taagaaaatt tagaacagtt gtaaatagaa
     2881 aacgttccaa aatctgaact gcaccccaaa agttagaggt gaaatctaat ttttgaggtg
     2941 cttttcttgt aaggtcaaga cacaaagaca agattgaaat ttatcgcttg caatgatatg
     3001 aaggagttga ttctatattt tgttttatca atactagttt gaatgttatc cgagaatttg
     3061 taggacattc cgaggatagt aaggagatag agaaaattta tcttcatgta atgcagaaag
     3121 gaaaacataa gattgaacag gctatgcaag atttagcaga aattatcatt tgatttattt
     3181 taatcagtta aaattcagtt attttgtcaa aaacaaaaga aaaaagcctt taaaacattg
     3241 atttcatagg atttctgtat attgtcttat tttacttatg taaaggttaa atattatctt
     3301 atcccagttg ttgaaaatcc ttttataata aggattttcg aaaatttgtg acgatcattt
     3361 ttgtgaaagg tagtgacaaa ggtagtgatg taattaagct gtcagttctt tcattttttg
     3421 agtaaagatg ttcacagcgt tttgaatttt tttaggagct agttcagcgt aggtatccat
     3481 tgttgtagat attgatttgt gccctaaacg tgtctgcaat tccttccaat ttgctcctgc
     3541 attcaacatc aatgaagcat gtgtatgccg aaagagatga aatccatact taggtaagtt
     3601 gattgattcc aatctttttt ctagagttgt acgctcgttc ctatcgcaca cataattccc
     3661 gtatatagtt ggaaaattaa cgggctttct ggcaaccctt tcttggtgaa atatgcattc
     3721 atttccagat gaaattctcg gagctcctca agaacttgtc tagggactga gacagtccga
     3781 ttgctggcat cagttttcgg tgtttgctta cagataacct cacctttaat ccctaatttt
     3841 ttattcccag ctttccacat taatgtttta ttgacttgga tctcatttgt gttaaacagt
     3901 aaatcatcaa tgtcaagagc aagtaattca ttgattcgaa gggcagatgc tagtaatgtg
     3961 ttacaaatcg ttttaaaacg tcgattagca cgtgtatttt gaagactatt aaggaattgt
     4021 aaccattttg ctaagtcttc atcatgcaat accatcacac gttttttagg agctttaggt
     4081 tttggaggaa ttttaactat tttggcaggg ttagacgtca agatttctaa tgtaatcccg
     4141 tagtcgaata tatcactcaa tttatggatt actgcaccaa aatcttttgc ttctcctttt
     4201 ctagattttt tctttcctga cgcaactgat ttttgtgatt tctgtgctaa atagttcacc
     4261 cattgttgta tgtcagctga ggtaatttgc tctggtttat aatctccgaa ttttggaaga
     4321 atataagtgt ccaagtagcc tctaactctg tttaacgtat tgtgtgaact aacccatgtc
     4381 ttgtaattag caaaccaagc ttcggcaagt tctgcaagat ttgaaaccct gataactgtt
     4441 ttgaccgttg aaccattaga ctcaaattcc aattttaatt caacgatttt cctatctagt
     4501 tgtcgaagtg tttttgcggt gactgttttg gtaattgact ttccagcctt actgtcagta
     4561 ccgatataga cacctttttg tgaatagctt atgctaccgt ctttttcgtg gttattatga
     4621 ctttttgcct ttgtattcaa ttgtttcttt attcatgttg tctcattaat atcggataag
     4681 taataaggct aaaataacaa ccctcacagt cttttttgat aatattattt tagcacattc
     4741 ttacttattt tgaaagaaag tcaataacat ctgatatttt gtagaacaca gttcttgtat
     4801 tttctattgg tggttctaat cttgataagc cctttttctc ccatgatttc aaagtatttg
     4861 gagagatttg taattgctgt aataattctt tttgtgttaa aattggtaac aaatattgag
     4921 cagcatacat tgattccaac tgttattttt cagcttttaa gaactccaac aactgattgt
     4981 ccaagaattt aaaaagaagg aggtcgctaa accagatggt cgtaaccaaa gtctttcaga
     5041 ttaaacaaac caaaaactta aaacgagcca ttgattacat tacaagagat gatgcaactc
     5101 tgattaaagt tgccaaacat gaacaggatg acgcattcga ctatacgctt tcggacagtg
     5161 gcgagattca caaaaaaatc atctcgggtc atcatctcat tgattcaagt aatcgagatg
     5221 ctatttatga tgatttcatt ttattaaaac agtccgttga tgaattttac gataatgata
     5281 cgctgtctga tttaaaaaat gacaaaagag tttactcaca caaaaagagc acaagatttt
     5341 taaggtcttg tgcttttttt gtgactttac aatgattcta caatcagtct aaaaatttaa
     5401 actagcttta ttgtttattt gttatcttga gaagctcttt tgatatagcg atacaaagta
     5461 ggaactgaaa tatgaagttg attggcagtt tcagcgacag ctcctttcat cgataagacc
     5521 ccttcctgtt ggatggtctt aatgaccata attttctcat ctactgacat tcttttagga
     5581 agagtccgag tgttgagaat agtattctga atacgttctt taataatttc atccatggca
     5641 ttggattcaa atgtctctga ataagtacta tcctctggga gagaaagatt gtagccttgc
     5701 aatagactct taatagctaa atttaactca gatacactat ttgtatcctt attaatgcat
     5761 agtagtccaa ttaatttttt ctggttcttg ataaaaaatg tggaggatag gaagttggca
     5821 gactttgagc tgccgttata attggagatg aaatctttat tttcatactc cttgttggca
     5881 atcatctgtc ggaacatatc tgtcaatgga gcccctacag ttcgtcctga aacaggattt
     5941 tctatagcga ttagtgagca gtcaggtttt cttatgtcat gaataacgat ttcggtgtta
     6001 cttccaagta cttgttgtag aaaaggaagc aattgaatat aaatatttaa caattcttca
     6061 tcagtcattt tttcattcct cctattaaaa tctttttaca attgatatga tagctcaaaa
     6121 cgagagaaaa agaaagagga aagataaaaa aatatcaaaa agataaaaaa actattgaca
     6181 tatatgaata aagaattata ataaaaagtg aaataaataa tatcaacaaa ataaaaatat
     6241 tatcaataac aaaaaggagg ctatcatgaa tatagcaatt aaaactaact tggttccgga
     6301 ggccattggt ccctattcta ccgccataga aacaaaagga aattgtcaaa aaacagttta
     6361 tgtttcggga caattaccaa ttgatgttgc gacaggtgag tttgcaggtt cggatataaa
     6421 ggaacagaca acacaatcgt taaaaaattt acggggcatc cttaatgcag ctgatatgga
     6481 tttgagtgat gttgtcaaaa caacagtctt cttatcggat attactgagt ttgcagctat
     6541 gaatgaagtc tatggagaat tctttgcgga tccctatcct gctagaagtg ctttccaagt
     6601 tgcggcgctt ccaaagaatg cacgtgttga aattgaagcc atagctgtta aatcaaatta
     6661 aatggaggag aacaaaaatg ggcaaatata attttgatga agaaattagt cgttatggat
     6721 caagttgtat taaatatgac tatggtatgc aacgtatggg acggacggat cttttaccta
     6781 tgtgggtagc tgatatggat ttcaaattac cagaagaaat cctagatgtc tttagaaaac
     6841 gaattgacca tggaatcttc ggctattctg accctgatga agactactgg caagccatga
     6901 atcattggtt cggtacacgt catggattta cgattgatcc tgaaacagtt acgctaggtg
     6961 ctggtattgt ttatggtttg gcaactgcag ttaaagcata ttctgaacca ggcgaagctg
     7021 tattgatcca acaaccagtt tactacccat tccgtgatgt tattgagaaa aatggccgca
     7081 aatttgttaa tagtcaattg atttatgatg atggacatta tagtatcgac tttgaagatt
     7141 ttgaacaaaa aatcattgat aataatgtca aggtatttat tctctgtaat cctcataatc
     7201 ctgttggacg tgtttggaca aaagaagaat taactcaatt agctgatatt tgtctcagac
     7261 acaatgttgt gattttatgt gatgaaattc actgtgactt tgtttatcct ggacataaaa
     7321 ccaccaattt tatgacctta gataagaaat accatgatag cttagtgctc ttcctatcac
     7381 cttcaaaaac ctttaatgta gcaggtttcc aaccagctaa tattgttatt ccaaacccag
     7441 aattacgctc taaatatcgt gcttcaaatg attctgccgc ttattcaaat gcttcactca
     7501 tggggatgtt agcagtaaaa gcttgttata ctcttggtgg tgactgggtc gatgaaatgg
     7561 tagactatct ctatggtaac atggtctata tgcgtgactt tgtgaaggat aacttcccta
     7621 aagctacatt tgttgagcct gaaggtacgt acctaatttg ggtcgatttc tctggttatg
     7681 gttttagtga tgaagaattg caacatatta tggttgaaga agcaaaattg tggttagatt
     7741 caggtaaaat ttttggtcct gcaacagctc aatttgaacg ttttaatatt gcctgcccac
     7801 gttccactgt cgttaaagct atggcacaat taaaagaagc gatggataaa catgaaaaat
     7861 gggagcagta aggagaatta taatcatgaa aaacatttgg aatacgtata aatcatcatt
     7921 tattctgtta gcctccatgg ttattggtgg agctattggt ttattttggg gaccagatgc
     7981 agctattctt cagccagttg cagatacttt ccttcactta ttgtattgtt gtgttgttcc
     8041 cttaatcttt tgttctcttt cagcagctat tgcaagaatg gaagatttaa ccaaactgaa
     8101 aaagatcttg ggtgttttct tagccggaac atttatttca ggtattattt cttgtctttt
     8161 tatgctcatt cctagtatga tttttgaccc cgctgcaggt tcaactgtta gtctgactga
     8221 aacagttgaa gatgcttccg gtagtatgaa tattttggga atgtttactg ttggggattt
     8281 tccggaactc ttctcccgct ctaatctaat ggctcttatc gtctttacag taatctttgc
     8341 ttttgctctt attcaagtcg gagaaaaagc tcacggtatc gtcaaaggcc tagaaaatat
     8401 gaccgaggtt attgttaaaa ttattggtat tgtcatgaag attgctccac ttggactagg
     8461 ctgttatttt gctattttgc tcggcacaaa tggtagtgat gtgattgctc cattatctaa
     8521 agcaattctc atgtatatgg tcgtaattat catctacttt atcgtttcgc aaacagctta
     8581 tgcatacatc ggagctggta aagaaggagt gaagagatgg tgggcaactg ccgttaaacc
     8641 aacattaaca gctctaggaa cttgttcctc tgcagctact ttaccagtaa atattgaaca
     8701 agcgaaacaa ataggtatcc ctgatgaaat tactaactta gtcgtgccac ttggagccaa
     8761 cctacataaa gatggtgctt gcgttattca aatcctaaaa atagctttcc tttgttcagt
     8821 attcaatatt gaatttgtga ctcctcgcaa tattctaata gccattgcgg tatctgtcat
     8881 tgtttcagta gttatgggag gtatccctgg tggtggttat gttgctgaaa tcttcattat
     8941 gtcagccttt ggattcccag cagtagcgat tccaattatg gttctcatcg gtaccattac
     9001 tgatgctcct gcaacagcag ttaatgttac tggagataca ggtttagcta tggttgtgac
     9061 tcgctttgta gaaggaaagg attggttaaa gaaatcttaa ttttttaaaa ctctactttc
     9121 tgagtagtct ccaggtgaat tagtgtcaag attaccgaag aacaatttta tttatgttct
     9181 ctataaagta acgtagctga gtgttactca taagaaaaga gcacaagatt ttaaggtctt
     9241 gtgctctttt cgttaaagga taggaaaaac aaggttttct tgggggtcag aaaaaacttt
     9301 cgatttcatt tgaagatttt ccatatttag tgatttggtc aactacaaat cagagtccct
     9361 ttatcgcatt agaaccatgg agcggcctct ctacatcact ggaagaatct gacatattca
     9421 atgccaaacg aaatattagt tacgttgctc caaaagaggt tgatacaaaa tattttgata
     9481 ttattattga gtaacctttt gaaaatgttt gttatttaca gaaatttggt aaaatagttc
     9541 gaataatgaa aaaactaaaa aatattaagc cacgatggtt tgtttttatt tgtatcctaa
     9601 tttttcttgc tttaggtttt gacagtcggc ttcaagtaac tcattacagg cttgttagtc
     9661 ccaaaatatc tcagccaatc agacttgctc taattactga tttacactca tcaaattacg
     9721 gtaaaaacca gatacaaatc ttggaagtat tagagaaaga aggcccagat atcattttat
     9781 taggtggaga tatttttgat gatgaacttt ctgacagaaa aactctagaa ttgttgagcg
     9841 ggctaagtaa atttaatcat acttactatg tgttaggtaa tcatgaaata tggagtaatc
     9901 gatatgaaca gatgctagaa catctgcgac aacagaatgt agccatatta tctggaaaaa
     9961 gtagtagagt tgatgtcagg gggcagtcca tcactatttc aggcattgat gatcctgaat
    10021 attttcaaaa gtatgatact agtgagagtt tgctagacca acttgaagac ataaaaggga
    10081 agcaaataag cactgacttc caagttttac tagcgcaccg accagaacta attgatactt
    10141 atcaaaagta tggatttgat ttggtgttat ctggtcatgc gcatggagga caatggagag
    10201 tgccttattt ggttaatggt gtatttgcgc ctaatcaagg ttttcttccg aaatatgcag
    10261 gtggtttata tgaagttgaa aattcacatt ttattgtaag caggggtctg gccaaagaat
    10321 ctacacgagt accaagattt tttaatcgcc cagaaatcat cattgtagat cttgttaatc
    10381 agtaacctcg ctcttttcta atcactcaaa aacccactga cttaattagc cagtgggttt
    10441 tattacttat ccgaatcaaa atcttatttc aaaaaaggta gtgattagac tgatttgtac
    10501 tgcaatgtaa tgaaagttca tctttcaaaa attgcttaaa atcagggctt ctaatgtcta
    10561 ttgacgtgta gtgaatccct atttcacctc agtaaaaatc acgtgcttac gcaattttgg
    10621 tgagtatttt ttcaattgaa gacggtctgg agtgttacgt ttgttttttg aagtaaggta
    10681 caaacgctca ccagattctt tgtgttcaag tgtaatattt acgcgcatgg tatctccctt
    10741 ctattattca gctgaagcag ccttagcgat tttacgtcct ttgtagtatc ctttcaaaga
    10801 tacacggtga gaacgtgagt aatctccagt agtttcatca aatttcacag ttggagctgc
    10861 tactttatag tgagtacgac gtttgttttt cttcgctttt gaagtgcgac gtgcaggtac
    10921 tgccatttct ttcttcctcc attaaatgtc agggccaagc cctttttcaa tctaaaataa
    10981 tgcgtatgac tttcgccaac tttagtagta taacaaaagt tttttgtaaa gtaaagttac
    11041 ttgacagatt ttttgaaaaa aatttgttca attgtatttt cctattttta tttaagtatt
    11101 gcacttaatt tttcttccc
//
LOCUS       DATUCG010000082        11067 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.084, whole genome shotgun sequence.
ACCESSION   DATUCG010000082 DATUCG010000000
VERSION     DATUCG010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11067
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.084"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(<1..365)
                     /locus_tag="VCA20_001651"
     CDS             complement(<1..365)
                     /locus_tag="VCA20_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1835437.1"
                     /translation="MDKKEMPTSVLIPRLLQSMKHLLPRIAVAVCFAVLGQVVTIAIP
                     VMLVYRAFDALIGNPTPLWTLGILILLALLRGAFRYGEHYFGHYVAFHTLAAYRRLIF
                     AKLRALAPGKLDRQDNGSLL"
     gene            complement(358..2117)
                     /locus_tag="VCA20_001652"
                     /pseudo
     CDS             complement(358..2117)
                     /locus_tag="VCA20_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941225.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            complement(2246..3730)
                     /locus_tag="VCA20_001653"
     CDS             complement(2246..3730)
                     /locus_tag="VCA20_001653"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEP1835438.1"
                     /translation="MTLVYQSTRDAQNTVSASQAILQGLATDGGLFTPIFIPTVDLDF
                     SVLKDASYQEVAKLILSAFLDDFTADELDYCINNAYDSKFDTPVIAPVVKLKGQYNLE
                     LFRGSTIAFKDMALSILPYLMTTAAKKHGLENEIVILTATSGDTGKAAMAGFADVPGT
                     QIIVFYPRDGVSKVQELQMTTQTGANTHVVAIDGNFDDAQTNVKHMFNDEALRAKLAA
                     KKLQFSSANSMNIGRLVPQIVYYVYAYAQLVKTGEIAAGDKVNFTVPTGNFGNILAAY
                     YAKQIGLPVGKLICASNDNNVLTDFFSTGVYDKNRTFRVTTSPSMDILVSSNLERLIF
                     HLFGNDAAKTAELMEALNTTGQYDIQGADAEILSLFAAAFATEEETAAEIKRVYDESD
                     YIEDPHTAVASAVYKQYVEQTGDQTPTVIASTASSYKFPVVAVEAVIGQSGLSDFEAL
                     AKLHEISGVDLPPAVDGLETAPVRHNTVVAAADMQTEVERYLGV"
     gene            complement(4344..6995)
                     /gene="adhE"
                     /locus_tag="VCA20_001654"
                     /gene_synonym="adhC"
     CDS             complement(4344..6995)
                     /gene="adhE"
                     /locus_tag="VCA20_001654"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEP1835439.1"
                     /translation="MADKKVVSPEEKLVEARKHVDELVQKGLVALDEFRKLGQEEVDY
                     IVAKASVAALDKHGELAMHAFEETKRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEE
                     NEVTGLTLIAEPVGVVCGITPTTNPTSTAIFKSLIALKTRNPIVFAFHPSAQESSAHA
                     ARVVYEAAVAAGAPENCIQWVTKPSMEATSELMKHDGIATILATGGNAMVRSAYSCGK
                     PALGVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEVYDEFVTE
                     FKSYKTYFVNKKEKALLEEYCFGVKANSKNCAEGKLNADIVGRPAAWIAEQAGFSVPE
                     GTNILAAEVAEIGEKEPLTREKLSPVIAVLKVEGRAEGLEAARQMVEFHGLGHSAAIH
                     TEDAELAKEFGTIVRAIRVIWNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSISDNVS
                     AMNLLNIKKVGRRRNNMQWFKVPSKTYFERDSIQYLQKCRDVERVMIVTDRAMVELGF
                     LDRIIEQLDLRRNKVVYQIFSDVEPDPDITTVYKGTELMRTFKPDTIIALGGGSPMDA
                     AKVMWLFYEQPTVDFHDLVQKFMDIRKRAFKFPELGKKSKFIAIPTTSGTGSEVTPFA
                     VISDKANNRKYPIADYSLTPTVAIVDPALVLTVPAHVTADTGMDVLTHATEAYVSTVA
                     NDFTDGLALQAIKLVFENLESSVKNADFVSREKMHNASTMAGMAFANAFLGISHSMAH
                     KIGGRFHTVHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYKADEKYQDIARLLGLPC
                     STPEEAVAAYAQAVYDLGERIGIQMNLKAQGIDEKELKEYSRELALLAYEDQCTPANP
                     RLAMVDHMQEIIEDAYYGYKERPGRIK"
     gene            7448..8473
                     /gene="adhP"
                     /locus_tag="VCA20_001655"
     CDS             7448..8473
                     /gene="adhP"
                     /locus_tag="VCA20_001655"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEP1835440.1"
                     /translation="MKAVVVNPESTGVVVVEKELRPLEAGEALVQIEYCGVCHTDLHV
                     ANGDFGKVPGRVLGHEGIGIVTEIAPGVTSLKVGDRVSVAWFFQGCGMCEYCTTGRET
                     LCRTVKNAGYSVDGGMAEQCIVTADYAVKVPEGLDPAQASSITCAGVTCYKAIKEAHL
                     EPGQWIAIYGAGGLGNLAVQYAKKVFNAHVIAVDINNDKLELAKEVGADVTINGLEVE
                     DVPGYIKEITGGGVHSTVVTAVSKVAFNQAIDSVRAGGYVVAVGLPSEYMDLSIVKTV
                     LDGIKVVGSLVGTRKDLEEAFHFGAMGLVVPVVQKRPVEDAEAVFNEMVAGTIQGRMV
                     LDFCHSH"
     gene            complement(8554..9837)
                     /locus_tag="VCA20_001656"
     CDS             complement(8554..9837)
                     /locus_tag="VCA20_001656"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEP1835441.1"
                     /translation="MKLQKPKGTQDLLPQDSAKWQYVENFTRSIFKQYNYAEIRTPIF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGERHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PAKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSNNPATDVETIAMAYHFFEELGIKD
                     IRLHLNSLGNPESRAAYRQALIDYLTPLKDQLSKDSQRRLEENPLRVLDSKEKEDKIA
                     VENAPSILDYLDAESTAHFEAVKSMLDSLGIAYTIDTNMVRGLDYYNHTIFEFMTEVG
                     GNDLTICAGGRYDGLVTYFGGPETPAFGFGMGIERLILVLEKQGIELPLDTQLDVYIA
                     VLGQEANGGALDLVQAIRKQGFRAERDYLDRKLKAQFKSADVFGAKAIITLGGSEIES
                     GQVVVKNNQTRSQVETSLEALKTDFASILEELEKQ"
     gene            complement(9952..10653)
                     /locus_tag="VCA20_001657"
     CDS             complement(9952..10653)
                     /locus_tag="VCA20_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835442.1"
                     /translation="MKTVGKFFAKKLASLLVLLLVLGIGIFWFRNSILAWFQGTHKVQ
                     YEFVIKKFEAESKLVVAGAEEDTTANQTFENNKLKEWPDWTEPITKLFVGREMSVDIP
                     VQTEFKLVLEGIGQSDVQIQNNTLTFKKPVLVEVDSQQHGEIKISNNSNGLLDKAVDV
                     FTSGQKAQEFLNDKSQEAIYKTSEEVLNDTGRQEKVAAFAETALENLLNLNSEEKLEV
                     EIEVADLNFQIVDKK"
     gene            complement(10926..>11067)
                     /locus_tag="VCA20_001658"
     CDS             complement(10926..>11067)
                     /locus_tag="VCA20_001658"
                     /inference="COORDINATES: protein motif:HMM:NF012948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEP1835443.1"
                     /translation="QGITYSRVDRAKALPETGSEDNIILVGIGAVLGGLGLAGARRRK
                     QG"
BASE COUNT         3048 a         2545 c         2345 g         3129 t
ORIGIN      
        1 agcagactac cattgtcctg tcgatccaat ttcccaggag ccaaggcccg cagttttgca
       61 aaaatcaagc gacggtaagc cgccagcgta tgaaaggcca cataatgacc aaagtaatgc
      121 tcaccatagc ggaaggcacc acgtaaaaga gctaggagaa tgaggattcc caaggtccat
      181 agcggagtag gattgccaat aagggcatca aaagcccgat agaccagcat aactggaata
      241 gcaatggtca ccacctgtcc caagaccgca aagcaaaccg ccaccgcaat ccgcggcaag
      301 aggtgcttca tggactgcaa gagtctcgga atcagcacac ttgttggcat ttctttctta
      361 tccataaact gcttcctcca attctctttg ttgatcgacc agctgaccat aagctgagct
      421 agtctgataa agcgtggcat gactgcctgt cacagctccc tcctcagaca agaagagaat
      481 atcatctgcc cgctccactt ttttcatctt atgggtaatg atgatgacaa tcgcatcctt
      541 ggccaccaag ttaatgaggt cgtaaatcaa gccttcattg tcctgatcga cgctggacgt
      601 cacctcgtca aagatataaa gggagcgttt ggccaagacc gcacgggcac agataacctg
      661 ctggcgttga ccaggcgaca agaaagcacc gtcatcaccc acaatcgtgt ccaacccatc
      721 tggcagattt ttaacaaact gcaagacccc atgctgatct gtccaggcca agatgtcctc
      781 cttggaccaa tcaaccgcca tccgcaagtt atggtagatt gactggttga gtagggttga
      841 ctgaccagat acatagagga cctgctcatt cagagacaac tggctgacag tcgaaatctc
      901 ctgctcaccc aagtaaatag ctcccttatc cgcccgcaag cgtcccaaga gcaactgggc
      961 caaggtcgtt ttcccctgcc ctgactcacc tgccaaggca tagaccttac cagctgtcat
     1021 ggtcatggaa atatcttcta ggactggctt ttctccgtag gcaaaggcca aatcttctag
     1081 ttttagactg ccaaaagcag gcacatggac agaatcgatt tcctgctccg ctgtcatact
     1141 gtccaagaaa ccaaaaatgc gatccgccat cttggtattc atcatgacca agtgcatacc
     1201 gtagccctgc tcccgtatgg gtgcaaaaaa ctccgtcgca atgaggacaa agaagagcat
     1261 actaaagagg gacaaattgc ctgccgctag gctattgacc gccacaaaac cagacaagcc
     1321 aatccccaga tacatgaccg catccatgta gcccaccgcc tgcaactgga agctaagtag
     1381 ctccatggtc gcatcgcgga agtcctcggt ctgctcattg aaggttttct cataagtcgc
     1441 gtccgcctga taggaataga gggtgttgag ccctttgagg tcgtccagga aaagattgcc
     1501 cacatccatg taggatcccc agtagcgatt catgatccgc ttggaccgct tctgcatagc
     1561 gataatggaa atgggaatta ggggcaaggc caatatgaaa atgactgcac ccaatggaaa
     1621 aattaggaag accagtaaga gaaccgtcgc acagttgaac tgggtccgca aggaagaagc
     1681 catatagtaa gaatagtagg tatccaggct atcaatcccc tgtgaagcga tgttaaagac
     1741 atccgccgcc gttgctttgg attcaaactg accatcctta gccaagaggg cctcaaagaa
     1801 ggactgctta agcgaatccc gtgcaaactg gagcccagcc cctgcaaacg cttggcgatc
     1861 aaagccacac cataaccaaa ggcattgagc cccagcaaga tgaggacaaa ctgtactaaa
     1921 ttaacctgcc cgccctcata gtaggacaca aaacccttag caatcaggta gaaggaaaca
     1981 atccgcatca aaaattgcac ccatgataga aaggcagcca aatagaccag cttcatcttg
     2041 ggcttgatgc gttctttgag ccgcttcaag agggcttttt tcttttcacg ggaaatcttt
     2101 tcttgttcct gtgccatcgt ccacctcact cttacgtatt cctatatagt atatgattct
     2161 ttgaattgtc tgtaaatttt aatttaaacc acaaaaagaa aacaaactgt tcaaaatgag
     2221 cagtttgtca taaaataaat cgaaattaaa cacccaaata gcgttctact tcagtctgca
     2281 tatcggcagc tgcaacgacg gtattgtggc ggacgggtgc cgtttccaag ccgtccacag
     2341 ctggtggcaa gtctacacct gaaatctcat gcaattttgc aagggcttca aagtcagaca
     2401 aacctgactg gcctatcacc gcctctaccg caaccacagg gaacttgtaa gaacttgctg
     2461 tagaagcaat cactgtcgga gtttggtccc ctgtttgctc cacatactgt ttatagacag
     2521 ctgaggcaac agccgtatgt ggatcttcaa tgtagtctga ctcgtcatag acacgcttga
     2581 tttccgcagc ggtttcttct tctgtcgcaa aggcggcagc aaagagagag aggatttccg
     2641 cgtcagcccc ttgaatgtca tactgaccag ttgtgttcaa ggcttccatc agctcggctg
     2701 tttttgcagc gtcattgcca aagagatgga aaatcaagcg ctccaagtta gaggacacca
     2761 agatgtccat ggacggactg gtggtcacgc ggaaggtacg gttcttgtcg taaacacctg
     2821 tcgagaagaa gtcggttagg acattgttat cgttggaagc acagatgagc ttgccaactg
     2881 gcagaccgat ctgcttagcg tagtaagccg ccaagatgtt tccaaagttt cctgttggaa
     2941 cggtgaagtt gaccttgtcg cccgccgcaa tctcaccagt tttgaccagc tgagcgtagg
     3001 catagacata gtaaacaatc tgtggtacca agcggccgat gttcatagag ttagcagatg
     3061 aaaactggag tttcttagct gccaatttgg cacggagagc ctcatcgttg aacatatgtt
     3121 tgacatttgt ctgggcatcg tcgaagttac catcaatagc caccacgtgc gtgttggctc
     3181 ctgtctgcgt agtcatctgc aattcttgga ccttagacac cccgtcacgt gggtagaaga
     3241 caatgatttg cgtaccaggg acatccgcaa atccagccat agccgcctta cctgtatcgc
     3301 ctgaagtcgc tgtcaaaatg acaatctcat tttccaaacc atgttttttc gccgcagtcg
     3361 tcatcaagta agggaggatg gacagagcca tatccttaaa ggcaatggtt gagccacgga
     3421 aaagctccaa gttgtactgg cccttaagct ttacaactgg ggcaataact ggcgtgtcaa
     3481 acttgctatc gtaggcattg ttgatacagt agtccagctc gtctgctgta aaatcatcca
     3541 agaaagctga caaaatcagt ttagcaactt cttgataaga agcgtctttc aaaacagaaa
     3601 aatccaagtc aactgttgga atgaaaatcg gtgtaaacaa accaccatct gtcgccaagc
     3661 cctgcaaaat cgcctgacta gcagatacag tattctgagc atcgcgtgtt gattgataaa
     3721 ctagtgtcat ggtatccctc gtaaaaatct attttttcta ttctaccaca aattgacaga
     3781 aaattgtgaa agatagatag aaaagtctct ttttctcggc taaaccctaa ctttccaacc
     3841 actttcgctt tttacagaat ctccaccatt atttctaaac aaacaatccc ctatttttca
     3901 atagaggatt gtcttacgat atttggaggc aatttaccta taaatcatac tccgttcaac
     3961 tgagtaagat acgatttgct actcctacct agactttttc tagtcctaag cctttctagg
     4021 cagattgacg aaggtgaagc taaatcacta aggaatgatt ttctaaacga gacaaggcaa
     4081 agcgacgtag acataactaa agttaggcaa ggagctttaa cgatgtatcg tgaagcaagt
     4141 tatcctcaat caagaaagga cttgtgcaac aacacttaca tgttcatttt gaaatcagtt
     4201 tttcctaaga gagtctagac ggcgaaggcg acggttgaac tcaaaccatc aaggaatgat
     4261 tttctaaacg agacaaggca aagcgacgta ggcataacta aagttaggca aggagcttta
     4321 acgatgtatc gtgacgaaaa tccttacttg atgcgacctg gacgttcttt gtaaccgtag
     4381 taggcatctt cgatgatttc ttgcatgtgg tcaaccattg ctagacgtgg gttagctggg
     4441 gtacattgat cttcgtaggc aagaagagcc aattcgcgag agtattcttt gagttctttc
     4501 tcgtcgatac cttgtgcttt caagttcatt tggataccga tgcgttcacc gaggtcgtaa
     4561 actgcttgtg cgtatgctgc aacagcttct tctggtgttg agcatggcaa gcctagaaga
     4621 cgagcgatgt cttggtattt ttcatctgct ttatagtagt tgtacttagg ccatgtagct
     4681 gtcttggctg gacgagtacc gttgtagcgg ataacgtatg gaagcaagat agcgtttgta
     4741 cgaccgtgaa cggtatggaa acgtccacca atcttatgag ccattgagtg agaaatacct
     4801 aggaatgcgt tggcgaaggc catacctgcc atagttgatg cgttgtgcat tttctcacgt
     4861 gatacaaagt cagcattctt cactgagctt tcaaggtttt caaatactag tttgatagct
     4921 tgaagtgcta aaccatctgt aaagtcgttg gctactgttg aaacgtatgc ttctgtagcg
     4981 tgagtcaata cgtccatacc agtatccgct gtaacgtgtg caggtactgt caatacgagt
     5041 gctgggtcaa cgattgccac agttggtgtc aatgagtagt cagcgattgg gtatttacgg
     5101 ttgtttgctt tatcagagat tacggcgaac ggtgttactt ctgaacctgt acctgaggta
     5161 gttggaatcg cgataaactt agatttctta ccaagttctg ggaacttgaa ggcacgttta
     5221 cggatatcca tgaatttttg aactaggtca tggaaatcaa ctgttggttg ttcgtagaag
     5281 agccacataa ctttggcagc atccattggt gagccaccac cgagtgcgat gattgtatct
     5341 ggtttgaagg tacgcatcaa ttctgtacct ttataaaccg ttgtgatgtc tggatctggc
     5401 tcaacgtctg agaagatttg ataaacaact ttattacgac gaaggtccaa ttgttcgatg
     5461 atacgatcta agaatccaag ttctaccatg gcacggtctg taacgatcat gacacgttca
     5521 acgtcacggc atttttggag atattggata gagtcacgtt cgaagtatgt tttagaagga
     5581 actttaaacc attgcatatt atttctacgt cttcctactt tcttgatgtt gagcaagttc
     5641 atagcactta cgttatcact gattgagttg cgtccgtaag aaccacaacc aagtgtcaat
     5701 gatggcaaga atgcgttgta aacatcaccg ataccaccga aagtagatgg agagttccaa
     5761 ataacacgga tagcacgtac gattgtacca aactctttcg ccaattctgc atcttccgta
     5821 tgaatagctg cagagtgacc gagaccgtgg aattcaacca tttggcgagc tgcttcaaga
     5881 ccttctgcac gaccttcaac tttcaagaca gcaataacag gtgacaattt ctcacgagtc
     5941 aatggttctt tttcaccgat ttcagcaacc tcagctgcca agatgtttgt accttctggt
     6001 actgagaagc cggcctgctc agcgatccat gcagctgggc gaccaacgat gtctgcgttc
     6061 aatttacctt cagcacagtt tttgctgtta gctttcacac cgaagcagta ttcttcaaga
     6121 agagcttttt ctttcttatt aacgaagtaa gttttgtaag atttgaactc tgttacaaac
     6181 tcatcgtaaa cttctttatc aatgataact gcttgttctg acgcacagac cataccgttg
     6241 tcaaatgact tagacataac gatgtcatgt gctgcctgac gaatatttgc tgatttttca
     6301 acataggctg gaacgttacc tgcacctaca ccaagggcag gtttaccaca agagtaagct
     6361 gaacgcacca ttgcgttacc accagttgca aggattgttg caataccatc atgtttcatc
     6421 aactcagatg ttgcttccat agatggtttt gttacccact ggatacagtt ttcaggagca
     6481 ccagctgcca cagctgcttc atagacaaca cgagctgcat gagcagaaga ttcttgcgca
     6541 gatgggtgga aggcaaagac aattggatta cgtgtcttca aggcaatcaa tgatttaaag
     6601 attgctgtag aagttgggtt tgttgttggt gtgataccac aaacaacacc gaccggctca
     6661 gcaatcaaag tcaaacctgt cacttcattt tcttcgataa caccaacagt tttcgtgtgg
     6721 cgcatattat tgacaacgtg ctcacaggca aacaagttct tagtagcctt gtcttcgaat
     6781 acaccacgct tggtttcttc aaaggcgtgc attgccaatt cgccatgttt atcaagggct
     6841 gcaaccgaag ctttcgctac aatatagtct acttcttctt gaccaagttt acggaactca
     6901 tcaagagcaa ccaaaccttt ttgaacaagc tcatcgacgt gcttgcgcgc ttctaccaac
     6961 ttttcttctg gtgatactac ttttttatca gccattgtgt tcctccaaat atactcgcta
     7021 accatttgtt aactttttca caatgttatt ctactatttt ttattttatt tgtaaagaga
     7081 aattgttaat tttttcacat tttttctgtt ttgttataga aagcgcttgc tctttcctgt
     7141 tttcagaaaa cattcagatt tgtttctgct ttgtttggtc gaaaaataca taaagtaaga
     7201 caaatcttta tcatttattc tttctctaac atttacgata ctagtttatc aggaaaacgc
     7261 ttccatgtaa atagattttt ttgaaaatat tgtagtaatt acgtgataat tctcaatttt
     7321 acattcattt gtcccttctt ttctactctt gcatttttgt ctaaattgtg atattcttaa
     7381 caatataaaa caactatatt aacacatcca aaaatgtaca taaaatatta aaacaggagg
     7441 tctgcttatg aaagcagtcg ttgtaaatcc agaatctact ggtgttgtcg ttgtcgaaaa
     7501 ggaacttcgt ccactcgaag ctggtgaggc tttggtccaa attgagtact gtggtgtttg
     7561 tcacactgac ctccacgttg ccaatggtga ttttggtaag gttcctggtc gcgttcttgg
     7621 tcacgaagga atcggtattg ttacagaaat cgcacctggt gttaccagtc taaaagttgg
     7681 tgaccgtgtc agcgttgctt ggttcttcca aggctgtggt atgtgtgaat attgtacaac
     7741 cggtcgcgaa actctctgcc gtaccgtaaa aaatgcaggc tactctgttg acggtggtat
     7801 ggctgagcaa tgtattgtaa cagcggacta tgcagtaaaa gttccagaag gtcttgatcc
     7861 agctcaagca agttctatca cttgtgctgg cgttacttgc tacaaagcga ttaaagaagc
     7921 tcacttggaa ccaggtcaat ggattgctat ctatggtgct ggtggacttg gaaaccttgc
     7981 tgtacaatac gctaaaaaag tcttcaacgc tcatgttatc gccgtagaca tcaataatga
     8041 taaattggaa ctcgccaaag aagttggtgc agatgtgacg atcaacggac ttgaagtaga
     8101 agatgttcct ggatatatta aagaaattac tggtggcggt gtacactcta ctgttgtaac
     8161 agctgtttcc aaagtagcct tcaaccaagc tattgacagt gttcgtgctg gtggctatgt
     8221 ggtggctgtc ggacttccat ctgaatacat ggacctcagt atcgtgaaga ctgtgctaga
     8281 cggtatcaaa gtggtcggct cactagttgg tactcgtaaa gatttagaag aggccttcca
     8341 cttcggtgct atgggactag ttgttccagt cgttcaaaaa cgtcctgtcg aagatgccga
     8401 agcagtcttc aatgaaatgg ttgccggcac tatccaaggt cgtatggtct tagacttctg
     8461 ccactcacac taactcattc tccactattt tcaagaagcg attgtccaat cgcttctttt
     8521 ttgctttcta aaataaaaag aagcagcttt agactactgc ttctccaatt cttctaaaat
     8581 acttgcaaaa tctgttttga gtgcttccaa gcttgtttca acttgacttc ttgtctggtt
     8641 atttttaacc acaacctgtc cagattcaat ttcactacca cccagggtaa tgatggcctt
     8701 ggctccaaag acatctgctg acttaaactg ggccttgagc ttgcggtcca gatagtcacg
     8761 ctctgcacgg aaaccttgct tgcgaatagc ttgaacaaga tcaagcgctc cgccattggc
     8821 ctcctgaccc aaaaccgcga tgtaaacatc taactgggtg tcgagaggga gctcgatgcc
     8881 ttgcttttca aggactagaa tgaggcgctc aatgcccatg ccaaaaccaa aagctggcgt
     8941 ttctggtcca ccaaagtagg tcaccaaacc gtcataacgg ccaccggcac agatggtcag
     9001 atcgttacca cccacttctg tcataaactc gaaaatggtg tgattgtagt agtccaaacc
     9061 acgcaccatg ttggtgtcaa tggtgtaagc aatacctagg ctgtccaaca tagacttgac
     9121 cgcttcaaaa tgagctgtac tttcagcgtc cagatagtcc aagatagacg gtgcattttc
     9181 caccgcaatc ttgtcttctt tttccttgga atccaacaca cgcaagggat tttcttccaa
     9241 gcggcgctgg ctatccttgg aaagctggtc cttgagcggg gtcagatagt caatcaaagc
     9301 ctgacgataa gcggcacggc tctcgggatt tcccagactg ttgaggtgga ggcgaatgtc
     9361 cttgattccc aactcttcaa agaagtgata ggccatagca atggtttcta cgtctgtcgc
     9421 tgggttattg gaaccaaaac actccacacc aatctggtgg aactgacgga gacgacctgc
     9481 ctgcggacgc tcatagcgga acattggacc catgtaatag aacttagctg gcttttgtac
     9541 ttctggagca aagagcttgt tttctacata agaacggaca acgggcgctg tcccttctgg
     9601 acgtagggtg atatgacgct ctcccttgtc atagaaatcg tacatctcct tggtcacaat
     9661 gtccgtcgta tcgccaaccg aacggctgat cacctcgtaa tgctcgaaaa tcggtgtgcg
     9721 aatctcagcg taattgtact gtttgaaaat gctgcgggta aagttttcca cgtactgcca
     9781 tttggcagaa tcctgcggta gtaaatcctg tgttccttta ggtttttgta atttcatagg
     9841 tcttctcact tctctatatc ttgcccctta ttttatcata ttttagtctt ttttggaaat
     9901 ggcagagcag acaaaaagcc gtgcctactg acacgacctt gtttaaaaaa actatttctt
     9961 atccacaatc tggaaattca agtcggcaac ttcgatttca acctcaagtt tttcttcgga
    10021 gttgagattg aggaggtttt ctaaagctgt ttctgcaaag gccgctactt tttcttggcg
    10081 tcctgtgtca ttcaatactt cctcgcttgt cttataaatg gcttcttggg atttgtcatt
    10141 tagaaattcc tgagcctttt gtccagaagt gaagacgtcg acagccttgt ctagcaaacc
    10201 attgctatta ttgctgattt tgatttctcc atgttgctga gaatcaactt caacaagcac
    10261 aggtttcttg aaggttaagg tattgttttg aatctgaaca tcagactgac caatgccctc
    10321 caagaccagc ttgaactccg tctgcacagg aatatctaca gacatttcac gtcctacaaa
    10381 gagcttggta atgggctctg tccagtctgg ccattccttt aatttgttgt tttcaaaggt
    10441 ctggttggca gtcgtatcct cctcagcacc tgctacaacc agcttgcttt ccgcttcaaa
    10501 cttcttaatg acaaattcat attgaacctt atgggttccc tggaaccatg ctagaatgct
    10561 atttctaaac cagaaaatac ctatacctaa aaccagcagc aagactaaga ggctagccaa
    10621 ctttttcgca aaaaactttc caactgtttt catacagttc tcctttatat tgaataattg
    10681 gaacttgtcc tataactatt atatatatgc tgtcaagtat tttctttctt ttttgactat
    10741 tttagcaaag tcctagattt tttctatttt aacccttctc aataaacatt accattccaa
    10801 taacggtaaa agaaatcatt aaaaatttcc caaaactgtc ggaatagaac aagcttttca
    10861 ataattttgg caatccaaaa aatcttccta ctatctttag taagaagatt ttttatgttg
    10921 actaactatc cttgtttacg tcttcttgca cctgcaagac caagtcctcc taacacagcg
    10981 ccaataccca ccaaaatgat attgtcttca gaacctgtct ctggtaatgc tttcgcacgg
    11041 tctacacgtg agtaggtaat accttgt
//
LOCUS       DATUCG010000083        10716 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.085, whole genome shotgun sequence.
ACCESSION   DATUCG010000083 DATUCG010000000
VERSION     DATUCG010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10716
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.085"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(41..697)
                     /gene="phoU"
                     /locus_tag="VCA20_001659"
     CDS             complement(41..697)
                     /gene="phoU"
                     /locus_tag="VCA20_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEP1835444.1"
                     /translation="MLRAQFEEELGKLHNQFYAMGNEVLAQINNAVRAFTTHDRELAK
                     EVIEADKKINAFEVKLEKKSLEIIALQQPVSTDLRRVITVLKATSDLERMGDHAVSVA
                     KAAIRMKGEVRIPVVEEEIKKMGREVRQLVEETLELYLGDADMERAYEVAARDEVVNE
                     FYSTIQELTTEEIKKNPESLVAGRDYYQVLTYLERVGDYAKNICEWVVYLRTGDITEL
                     "
     gene            complement(726..1484)
                     /gene="pstB"
                     /locus_tag="VCA20_001660"
     CDS             complement(726..1484)
                     /gene="pstB"
                     /locus_tag="VCA20_001660"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEP1835445.1"
                     /translation="MTSPILQVKDLSVYYNKKKALNNVSIDFYPNEITALIGPSGSGK
                     STLLRSINRMGDLNPEVTLTGAIDYNGKNIYSPRTDTVDLRKEIGMVFQQPNPFPMSI
                     YENVVYGLRINGIKDKQVLDEAVERSLIGASIWNEVKDRLHDSAVGLSGGQQQRVCVA
                     RVLATSPKIILLDEPTSALDPISAGKIEDTLYGLKDKYTMVLVTRSMQQASRISDRTG
                     FFLDGDLIEYNLTKEMFLNPANKETEDYITGKFG"
     gene            complement(1496..2299)
                     /gene="pstB"
                     /locus_tag="VCA20_001661"
     CDS             complement(1496..2299)
                     /gene="pstB"
                     /locus_tag="VCA20_001661"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEP1835446.1"
                     /translation="MADYNWNERHIITFPEENIALSTKDVHVYYGKNKAIKGIDMQFE
                     KNKITALIGPSGCGKSTYLRSLNRMNDTIDIAKVTGQILYEGIDINRPDMNVYEIRKH
                     IGMVFQRPNPFAKSIYRNITFAHERAGVKDKQVLDEIVETSLKQAALWDQVKDDLHKS
                     AFTLSGGQQQRLCIARAISVKPQILLMDEPASALDPIATMQLEETMFELKKDYTIIIV
                     THNMQQAARASDYTAFFYLGDLIEYDKTANVFQNAQLKSTSDYVSGHFG"
     gene            complement(2324..3211)
                     /gene="pstA"
                     /locus_tag="VCA20_001662"
     CDS             complement(2324..3211)
                     /gene="pstA"
                     /locus_tag="VCA20_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEP1835447.1"
                     /translation="MHAKKFDKLATGILYSIAGVIVVILTSLILYILLRGLPHVSWDF
                     LTSKSSSYKAGGGIEIQLYNSFFLLVVTLLISVPLSTGAGIYLAEYAKKGPLTNFIRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSILSGALALTVFNLPQMTRNVEDSLRHVH
                     HTQREAGLALGLSRWETVLHVVIPEALPGIVTGIVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNPLSVTSPISIFRQSETLAVHIWKVNSEGTIPDATQVSAGSAAILLVFILIF
                     NLSARYIGKKLHAKLTSAA"
     gene            complement(3201..4118)
                     /gene="pstC"
                     /locus_tag="VCA20_001663"
     CDS             complement(3201..4118)
                     /gene="pstC"
                     /locus_tag="VCA20_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEP1835448.1"
                     /translation="MKNEQLAKELSSPSKNSRLEKFGKVITFLCLSLIVFIVAMILIF
                     VAQRGLSTFFVDGVSITDFLFGSKWEPSSKIFGALPMISGSFIVTILSAVVATPIAIG
                     AAVFMTEISPKRGAKILQPVIELLVGIPSVVYGFIGLQVVVPFVRSIFGGTGFGILSG
                     VFVLFVMILPTVTFMTVDSLRAVPRHYREASLAMGATRWQTIWRVILNAAKPGIFTAV
                     IFGMARAFGEALAIQMVVGNSAVIPTSLTTPAATLTSVLTMGIGNTVMGTVQNNVLWS
                     LALVLLLMSLIFNMIMKFITREGKKNYAR"
     gene            complement(4121..4990)
                     /locus_tag="VCA20_001664"
     CDS             complement(4121..4990)
                     /locus_tag="VCA20_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HEP1835449.1"
                     /translation="MKKKQKLGIAALFLLSSMALSGCSSWIDRGESITAVGSTALQPL
                     VEAAADEFGSVNIGKSVNVQGGGSGTGLSQVQSGAVQIGNSDVFAEEKEGIDASKLVD
                     HQVAVAGLAVIVNEKVTVDNITTEDLRKIFTGEITNWKELGGKDLEISIINRAASSGS
                     RATFDSVIMDGQTATQSQEQDSNGMVKSIVAQTPGAISYLSFAYLDDSVKTIKLNGFE
                     PTAENVATNDWPIWSYEHMYTMGKATDLTEEFLDYMISDEVQNGIVESMGYISINDMK
                     VVKSADGTVTEKE"
     gene            complement(5128..6429)
                     /locus_tag="VCA20_001665"
     CDS             complement(5128..6429)
                     /locus_tag="VCA20_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HEP1835450.1"
                     /translation="MQLPQDFIDKYNHLLGSEAQAFFESFEQDPISGFRTNPLKESQL
                     DFEQPIPNMTWSYYGKVSGKSSEHVTGLVYSQEPAAQVVGQIAAPEKGMKVLDLAAAP
                     GGKSTHLLSYLENTGLLVSNEISNKRAKILAENIERFGARNVVVTNESAGHLEKVFPG
                     YFDMIVLDAPCSGEGMFRKDPDAIQYWSKHYPAQCASLQREILESALNMLAPDGQLIY
                     STCTWSPEENEEVVTWLLEQYDLELIDIPKINGMVEGVGYPEVARMYPHHFAGEGQFV
                     AKFRFLGEASQKKLKPGKSNLNREQQALWKEFEQKHLRVQLDGLLQVFGDNLYLLPHG
                     LPDLSKVKIARNGLHLGKFKKKRFEPSFALGLALHSSEVKNRVEISLEEFSSYTAGHT
                     IAVSKDLANGWYQVAVQGNGLGFAKIVSGTLKNGFPKGLRF"
     gene            complement(6429..7187)
                     /locus_tag="VCA20_001666"
     CDS             complement(6429..7187)
                     /locus_tag="VCA20_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEP1835451.1"
                     /translation="METKYHFAQTIVLEAGNFLRQHLHDDLQIEEKGDFTDLVTHLDK
                     QVQEMLTQQIQSRYQSDGILGEEGGDVSSIHEGNVWVIDPIDGTTNFIAQKTDFAIMI
                     AYFENGVGKFGIIYDVMRDKLFHGGDSFPVYCNQERLKKPELRPLRQSLIGVNAKLYA
                     GNAHGVAELANQSLGTRSVGSAGIGFAHVLEGRLFAYASYLCPWDYAAASIIGSSLGL
                     VMLSFEGPSLAYDKREFVMLVSESHLEEVKGYIF"
     gene            complement(7177..7455)
                     /locus_tag="VCA20_001667"
     CDS             complement(7177..7455)
                     /locus_tag="VCA20_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEP1835452.1"
                     /translation="MNKNYSYPLDFSWSTEEISSVLSFLNQVEAAYEKGADAVAILAS
                     YKSFKDVVKSKGQERQIDRDFEAVSGYSTYRVVKAARDKGKGVIRFGN"
     gene            complement(7470..7871)
                     /locus_tag="VCA20_001668"
     CDS             complement(7470..7871)
                     /locus_tag="VCA20_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEP1835453.1"
                     /translation="MITLFLSPSCTSCRKARAWLTSHEVPFIEHNIMTSPLTAEELKN
                     ILALTENGTDDLISTRSKVFQKLDIDVDELSVKQLIGLIETYPSLLRRPIILDEKRMQ
                     IGFNEDEIRAFLPRKYRKQELKNATLRAEIG"
     gene            complement(7945..8682)
                     /locus_tag="VCA20_001669"
     CDS             complement(7945..8682)
                     /locus_tag="VCA20_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEP1835454.1"
                     /translation="MLKRLIRHPLYEKAMTYIAVVYVFLIVLELMDANIANSSGYRVL
                     DLLLWFVFVCDYFAQLYYADDILEYVQGHILELIAIIPFDLFFSFLRLGRLARLFRLV
                     KVTRIFALSNRFWDTINKLLHTNSLSKVLMLNISAVLVASVLLSVIEGKSFFDALWWS
                     IVTMTTVGYGDIVPQDTISKIIAILLMLVGICTFGMVTSTITRFFSETERETKLDTLM
                     AKIDEQNEILLRLEKKIESLEKLQGRD"
     gene            complement(8675..9598)
                     /locus_tag="VCA20_001670"
     CDS             complement(8675..9598)
                     /locus_tag="VCA20_001670"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEP1835455.1"
                     /translation="MKIRTIKNYQELHQEEPTVLVLGYFDGIHLGHKALLDRARQVAD
                     EKGLSVTVLTFPETPRLAFARFSPELLLHLTSQDQRYHLLEQYGVDQLVLTDFTSEFA
                     SNTPQQFLERYVKGLNAQVLVAGFDYHFGNCRADVKDLTELFDGQVEIVSEVSLGGEK
                     VSSTRIRQAIQSGDVSLANQLLGYPFMTEGIVVHGDARGRTIGYPTANLAPFDRVHLP
                     SEGVYVADVEVDGKRYRAMTSVGKNVTFDGTEMRIEAHIFGFDRFIYGEKITIFWLEK
                     IRDMVKFDGIEGLMEQMKSDEEFALQWEKYA"
     gene            complement(9618..10508)
                     /gene="truB"
                     /locus_tag="VCA20_001671"
     CDS             complement(9618..10508)
                     /gene="truB"
                     /locus_tag="VCA20_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006597324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEP1835456.1"
                     /translation="MSGIINVKKEAGMTSHDVVFKLRKILQEKKIGHGGTLDPDVTGV
                     LPIAVGKATRMIEYMQEEGKIYEGEITLGYSTTTEDASGDLVERTPVLDISEQAVDEA
                     MASFVGTITQIPPMYSAVKVNGRKLYEYARAGEEVERPQRQIEIYGFERTSPIELTDD
                     CARFTFRVRCGKGTYVRTLSVDLGAKLGYASHMSKLERTGSAGMSLADALTIEEIAEK
                     VAQNDYSFLRPIEQGIGDLPIVDLTIEQVIDARFGRFITLDSQAELLAGFHKEKLVAI
                     MEKRDQVYKPRKVFLEEVLA"
     gene            complement(10561..>10716)
                     /locus_tag="VCA20_001672"
     CDS             complement(10561..>10716)
                     /locus_tag="VCA20_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835457.1"
                     /translation="SLHIVYDEEAEEGIDEAVTFDAVPSGTMRRIHKELENLIGTTFG
                     SYRFYDD"
BASE COUNT         3215 a         2458 c         1915 g         3128 t
ORIGIN      
        1 aagtccatta cacaaaaaga agccctctcg gacttctgaa ttacaactct gtaatatcac
       61 ctgtgcgtag ataaacaacc cactcacaaa tatttttagc ataatcaccg acacgttcca
      121 agtaggttaa aacttggtaa tagtcgcgac cagcaaccaa ggattctgga ttcttcttga
      181 tttcttccgt tgtcaattct tgaatagttg aatagaattc attgacaacc tcatcacgtg
      241 ctgctacttc ataagcgcgc tccatatcag catcacctaa gtagagttcc aaagtttctt
      301 ctactaattg acgaacttca cgtcccattt tcttgatttc ttcttcaaca actgggatgc
      361 gaacttctcc cttcatacgg atagctgctt tagcaactga gactgcatgg tctcccatac
      421 gctccaaatc actagttgct ttcaagactg tgataacgcg acgaagatct gttgagacag
      481 gttgttggag agcaatgatt tcaagtgatt ttttctctaa tttcacttcg aaagcattga
      541 ttttcttatc tgcttcaatg acttcttttg ctaattcacg atcatgagtt gtaaaggcac
      601 gaacagcatt attaatctga gctaatactt catttcccat ggcgtaaaat tggttatgaa
      661 gttttcctaa ttcctcttca aattgagctc ttaacatgtg aacctctttc tttttcttat
      721 aggccttagc cgaacttacc tgtaatgtag tcttctgttt ctttattggc aggattcaag
      781 aacatttctt ttgtcagatt gtattctatc aaatcgccat caaggaagaa acctgttcga
      841 tctgaaatac gtgatgcctg ttgcatagac cgtgttacta gcaccattgt atacttatct
      901 ttcagaccat aaagtgtgtc ctcaatttta ccagctgaga ttgggtcgag ggctgaagtt
      961 ggctcatcca acaaaataat ttttgggctt gtcgccaata cgcgggccac acatacacgt
     1021 tgctgctgac cacctgaaag tccaactgct gagtcatgca agcgatcttt cacttcattc
     1081 caaatagaag caccaatcaa ggaacgctca actgcttcat ccaagacctg cttgtccttg
     1141 ataccgttaa tacgcaaacc ataaacgaca ttctcgtaga tagacatcgg gaatgggttt
     1201 ggttgttgga ataccatacc gatttcctta cgaagatcaa cagtatccgt acgaggactg
     1261 tatatgttct ttccgttgta atcaatcgca cctgtcaatg ttacctctgg gtttaaatct
     1321 cccatacggt taatagaacg caagagggtt gacttaccag aaccagatgg tccaatcaaa
     1381 gcagtaattt catttggata gaaatcgatt gacacattat tcaaggcttt tttcttgtta
     1441 tagtaaacag acaaatcttt tacttgtaaa ataggtgatg tcatttgttt cctttctatc
     1501 caaaatgtcc tgaaacgtag tcactagttg acttgagttg tgcattctgg aagacatttg
     1561 ctgtcttatc atattcaatc aagtcaccca gatagaagaa agctgtgtaa tcacttgcac
     1621 gagctgcttg ttgcatattg tgcgtaacaa tgataatggt gtagtccttc ttcaattcaa
     1681 acatagtttc ttctaactgc atagtcgcaa ttggatccaa ggctgatgca ggctcatcca
     1741 taagcaagat ttgtggtttt acagagatag ctcgtgcaat acacaaacgc tgttgctgtc
     1801 cacctgataa ggtaaaggct gacttgtgca agtcatcttt tacctgatcc caaagggctg
     1861 cttgtttcag agaagtttca acaatctcat ccaaaacctg cttatctttc acacctgcac
     1921 gttcatgtgc gaatgtgatg ttgcgataaa tcgattttgc aaatgggttg ggacgttgga
     1981 ataccattcc gatatgctta cgaatttcgt aaacgttcat atctggtcta ttgatgtcga
     2041 ttccttcata aagaatttga ccggttacct tagcaatatc aatagtatca ttcatacgat
     2101 taagactgcg aagataggtt gatttcccac accctgatgg tccaatcaag gctgtaattt
     2161 tatttttttc gaactgcata tcaatgccct tgattgcttt atttttacca tagtaaacat
     2221 gaacatcttt tgtcgaaagg gcgatattct cctctggaaa agtaatgata tggcgttcat
     2281 tccagttgta atctgccatt ttttctcctt ataagtcaga cttctaggct gctgaagtca
     2341 gtttcgcatg aagtttctta ccaatataac gtgcagaaag gttgaaaatc aagatgaaga
     2401 ccaagagaat cgctgctgaa ccagcagaaa cttgggttgc atctggaatt gtaccttcgc
     2461 tgtttacctt ccagatatga accgcaaggg tttctgattg acggaagata gaaatcggac
     2521 tagtaacact caatggattc cagtttgacc agtcaagagc tggagctgac tgaccagccg
     2581 tgtagataag agctgctgcc tcaccgaaga tacgaccaga tgccaagacg atacctgtta
     2641 caatccctgg aagtgcttct ggaataacaa catggagaac agtttcccaa cgtgatagac
     2701 caagagccaa tcctgcttca cgttgtgtat ggtgaacgtg acgaaggctg tcctctacgt
     2761 tacgtgtcat ctgtggtaag ttgaagactg taagagccaa ggcacctgat aagatagaaa
     2821 aaccgtactc aaactgaaca acgaaaatca agtaaccaaa caaaccaacg acaacggacg
     2881 gtagggatga caaaatctca atacaggtac ggataaagtt agtcaatgga ccttttttag
     2941 catattcagc caaataaata cctgcaccgg ttgacaaggg aacagaaatt aagagcgtta
     3001 caaccaatag gaagaatgag ttgtagagct gaatttcaat accaccgcca gctttgtaag
     3061 atgaagattt acttgtcaaa aaatcccagc ttacatgtgg cagaccacgt agcaagatgt
     3121 aaagaatcag tgacgtcaag ataacgacaa tcacaccagc gattgagtaa agaataccag
     3181 tcgccaattt atcaaatttt ttagcgtgca tagtttttct taccctctct cgtaataaat
     3241 ttcataatca tgttaaagat aaggctcatc aagagcaaga ctagggccaa ggaccatagg
     3301 acgttgttct gaacagttcc cataaccgta ttaccgatac ccattgtcaa aacagaagtc
     3361 aaagtcgctg ctggcgttgt caaggaagtc gggataaccg cagagttccc cactaccatc
     3421 tgaatcgcca aggcttcacc aaaggcacgc gccataccaa agataacggc cgtaaaaata
     3481 cctggcttag cagcattcaa gataacacgc caaatcgtct gccaacgagt ggctcccata
     3541 gccaaacttg cttcgcggta gtgacgaggt actgcacgca aactgtcaac cgtcataaag
     3601 gttactgttg gtaaaatcat aacgaaaagt acaaatacac ccgacaagat accgaaacct
     3661 gttccaccga agattgaacg tacaaatggc acaactactt gcaaaccaat gaaaccatat
     3721 acaaccgatg gaataccaac caaaagttcg ataacaggtt gtaagatttt tgcaccacgt
     3781 tttggtgata tttctgtcat gaagaccgct gcaccgattg cgataggggt cgcaacaaca
     3841 gctgacagaa tcgtaacaat gaaagaacct gaaatcattg gcagggcacc aaatattttc
     3901 gagcttggtt cccacttgct tccaaataag aagtcagtga tgctcacacc gtcaacaaag
     3961 aaagtcgata atccacgctg cgcaacgaaa atcaaaatca ttgcaacaat aaagacaatc
     4021 aatgacagac aaaggaatgt aattacctta ccaaacttct ctaatcgaga gtttttagat
     4081 ggagacgata actcttttgc tagttgttcg tttttcatgc ttactccttc tcagtcaccg
     4141 tgccatcagc acttttcaca actttcatat cgttgataga aatgtatccc atactttcaa
     4201 cgataccatt ttgaacttca tctgatatca tgtaatctaa aaattcttct gtcaaatcag
     4261 tcgcttttcc catcgtatac atatgctcat aggaccaaat tggccaatca tttgttgcta
     4321 cattttcagc agttggttca aaaccgttta gcttaattgt tttaacggaa tcatctagat
     4381 aagcaaatga taggtaggaa attgcacctg gtgtctgagc aacaatagat tttaccatac
     4441 cgttagaatc ttgttcttga ctctgagttg cggtttgtcc atccatgata acactatcaa
     4501 aggtcgcacg tgaacctgaa ctagctgcac ggttaatgat tgaaatttcc aaatccttac
     4561 cgcccagttc tttccagttg gtaatctcac cagtaaaaat tttacgcaga tcttctgtgg
     4621 taatattatc aactgtgact ttttcattga caatcacagc aagacctgca acggcaactt
     4681 gatggtctac caatttgctg gcatcaatcc cttctttttc ctcagcaaat acgtcagagt
     4741 taccaatttg aacagctcca gactgaacct gtgacaaacc agtaccagaa ccgccgccct
     4801 gaacgttgac ggatttaccg atattgacac taccaaattc atctgctgct gcctcaacaa
     4861 gcggttgaag cgctgtcgaa cctacggctg taatagactc tcctcgatcg atccaagaag
     4921 aacaacctga aagagccata ctactcaaaa gaaacaaagc tgcaattcca agcttctgct
     4981 tttttttcat ttcgtttttt cctttacatt tttcagtgga atctcctaaa attaactctg
     5041 tattttacat agaatttaca agatatttcc actcatccac tatatcatat aaaaagacaa
     5101 gttgcaaaaa aaatttgctg atttatttta aaatctcaac ccttttggaa agccattttt
     5161 aagcgttcca gatacaattt ttgcgaaacc aagaccgttc ccctgaactg ctacctgata
     5221 ccagccatta gctaaatcct tggacacagc tatggtatgt cctgcagtat aggacgagaa
     5281 ttcttctaag ctgatttcca ctcgattttt aacctccgaa ctatggagtg ctagacctaa
     5341 agcaaaactt ggttcaaatc tttttttctt aaacttcccc aagtgaagac catttcgagc
     5401 aatctttact tttgacaaat caggaaggcc gtgtggcagt agatagagat tgtcgccaaa
     5461 aacctgcagt aaaccatcca attgaaccct taaatgcttc tgttcaaatt ctttccagag
     5521 agcttgctgt tctctattca aattggattt tcctggtttt aattttttct gactggcttc
     5581 ccctagaaat ctaaatttgg caacgaactg accttctcct gcaaaatggt ggggatacat
     5641 acgagcaact tctggataac cgactccttc taccattcca ttgattttag gaatgtctat
     5701 tagttccaaa tcatactgtt ctaagagcca cgtcactact tcttcatttt cctccggtga
     5761 ccaagtgcaa gtagaataaa tcaattgacc atctggagcc aacatgttca aggccgattc
     5821 taaaatttca cgctgtaagc ttgcacattg tgctggataa tgtttggacc aatattgaat
     5881 cgcatcgggg tcttttcgaa acatcccttc acctgaacag ggagcatcta ggacaatcat
     5941 atcgaaatat cctggaaaga ccttttccaa gtggcctgct gattcatttg ttacaacaac
     6001 attcctcgct ccgaaacgct caatattttc agccaatatt tttgctcgtt tgttagaaat
     6061 ttcattacta accaagagcc ctgtattttc taagtatgaa agcaagtgag tagactttcc
     6121 tcctggcgct gctgccaagt ccaacacttt catccctttt tccggagctg caatctgacc
     6181 tacaacctgt gcagctggct cttgtgagta gactaagcca gttacatgct ctgaagactt
     6241 tcctgagacc ttaccatagt atgaccaagt catattaggg attggttgct caaagtccaa
     6301 ttggctttct ttaagaggat ttgtgcgaaa tcctgagatt gggtcttgtt caaaactctc
     6361 aaaaaatgct tgggcttcac taccaagcaa atgattatat ttatctataa aatcctgtgg
     6421 taattgcatt agaatatata ccccttcact tcctcaagat gggattctga tactagcatg
     6481 acaaactctc gcttatcata ggccagactc ggaccctcaa aacttaacat gactaagcct
     6541 aaagaggaac caataatact ggctgctgcg tagtcccagg gacacagata agaagcatag
     6601 gcaaataatc ttccttccag aacgtgggcg aaaccaattc cagcgcttcc tacagatctt
     6661 gtacctaaac tttgatttgc taattctgcc actccatgtg cattgcctgc gtacaacttt
     6721 gcattgactc ctatcaaact ctgacgaaga gggcgcagct ctggtttctt taagcgttct
     6781 tgattacaat aaacagggaa tgaatctcct ccatggaaaa gtttgtctcg catgacatca
     6841 taaataatac caaactttcc tactccattt tcaaaatagg caatcataat ggcaaaatca
     6901 gttttctgag caataaaatt tgtggtgcca tcaattggat caataaccca aacatttccc
     6961 tcatgaatag aagaaacgtc tccaccttcc tcacctaaaa tcccatcact ttgatagcga
     7021 gattgaatct gttgagtcag catttcttgc acttgtttat caaggtgcgt taccaagtct
     7081 gtaaagtcac ctttttcttc aatctgaaga tcatcatgta aatgctgtcg gagaaaattt
     7141 ccagcttcca ggacaatcgt ttgggcaaag tgatacttag tttccaaaac gaataactcc
     7201 tttcccctta tctcgagcag ccttaacaac tcgataggtt gaatatccag aaactgcttc
     7261 aaagtcacga tcaatttgac gttcctgtcc cttactttta acaacatctt taaagctttt
     7321 ataactagct aatatagcta ccgcatctgc acctttttca taagctgcct ccacctgatt
     7381 caaaaaagaa agcacggagg aaatctcctc tgtgctccac gaaaaatcaa gtggatatga
     7441 ataatttttg ttcatatcat ttatctttct tatcctattt ctgctctcaa tgtagcattt
     7501 tttagttctt gtttgcgata cttccgcgga agaaatgcac gaatttcatc ttcgttaaaa
     7561 ccaatctgca tccgcttctc atccaaaata atcggacgcc gtaagagact cggataggtt
     7621 tcgattaacc caataagctg cttcacagaa agctcatcaa catcaatatc tagtttctga
     7681 aaaacttttg aacgggtaga aatcaagtca tctgtcccat tttctgtcaa tgctaaaatg
     7741 ttctttaatt cctcagcagt tagagggcta gtcataatat tgtgctcaat aaaagggact
     7801 tcgtgacttg tcagccaagc acgagcttta cgacaactgg tacaactcgg tgacaaaaat
     7861 aaagtaatca tgtcttccat cccctctaaa aacatttttc ttcttatagt atactctaac
     7921 ttaaaagagt tttcaatagt aaatctaatc tcttccctgt aatttttcca aagattctat
     7981 ttttttctct aagcgtagca atatttcgtt ttgttcatct atttttgcca tcaaggtatc
     8041 caacttcgtt tcacgctctg tttcagagaa aaaacgagtt attgtagagg taaccatacc
     8101 aaaagtacaa ataccaacca acatcaagag gatggctata attttagaaa tcgtatcttg
     8161 ggggacaata tccccgtacc ctacagttgt catcgttaca atggaccacc aaagtgcatc
     8221 aaagaatgat ttcccttcaa tcactgatag taagacactt gcaactagaa ccgctgagat
     8281 atttaacatc aacactttgg ataaactgtt ggtatgcaat agcttgttga ttgtatccca
     8341 gaatcgatta gacagggcaa atattctcgt tactttgact aatctgaaga gtcgtgcaag
     8401 tcttcccaag cgaagaaaac tgaagaataa atcgaatgga ataatagcaa ttaattctaa
     8461 gatgtggccc tgtacatatt caagtatgtc atccgcataa taaagctgag caaaataatc
     8521 gcagacaaaa acaaaccata agagcaagtc tagtacccga tacccactag aattagcaat
     8581 attagcatcc atcaattcaa gtacgataag aaatacatag actacagcta tgtaggtcat
     8641 agccttctca taaagaggat gacgaatcaa tcgtttaagc atatttctcc cattgcaagg
     8701 caaattcctc atcggatttc atctgctcca tcaaaccttc aatcccgtca aatttaacca
     8761 tatctcgaat tttttcaagc caaaaaatag ttattttttc tccgtagata aagcggtcaa
     8821 acccaaaaat atgggcttct atccgcatct ccgttccatc aaaggtcaca tttttcccaa
     8881 cgcttgtcat agctcgatag cgttttccat ctacttcaac atcagcgaca tagactccct
     8941 cagatggtaa atggacacgg tcaaaaggtg ccagatttgc agttggatag ccaatggtgc
     9001 gtcctctggc atctccatga acaacaattc cctccgtcat aaatggataa cctaaaagtt
     9061 gattagctag agagacatcc cctgactgga tagcttggcg aatacgagtt gaagaaactt
     9121 tttctccgcc caggctcact tcacttacaa tttctacctg accatcaaat aactctgtca
     9181 aatctttgac atctgctcta caatttccaa aatgatagtc gaaacctgcc actaagactt
     9241 gtgcgttcaa ccccttgacg tagcgttcta aaaactgctg aggagtattg ctggcaaact
     9301 cactggtaaa atctgtcaaa accagctggt ctacaccgta ttgctccaac aagtggtaac
     9361 gctgatcttg actggtcaaa tggagtaaaa gttctggtga aaaacgcgca aaagccagac
     9421 gaggtgtttc tggaaaagtc agtacggtca ctgacaatcc tttttcatct gcaacttgtc
     9481 tagcacggtc taagagggcc ttatgcccca aatgaatccc atcaaaataa cctaatacca
     9541 aaactgtcgg ctcttcctga tggagttctt gataattttt tatcgttcta atcttcatat
     9601 tgtttatttt atcacaacta ggctaaaact tcttctagaa aaaccttacg aggcttgtaa
     9661 acttggtcac gtttttccat gattgccacc agtttctcct tatgaaaacc tgccaacaat
     9721 tctgcctgac tatctaaggt tataaaacga ccaaatctgg catcaataac ttgctcaatc
     9781 gtcaaatcaa caatcggcaa gtcaccaatt ccctgctcaa tagggcggag gaaagaataa
     9841 tcattttggg caaccttttc agcaatctct tcgattgtaa gggcatcagc caggctcata
     9901 cctgcggatc ctgtacgctc taacttagac atgtgactgg cgtagcctaa cttagcccct
     9961 aagtcaacag aaagagtacg gacataggtg cccttaccac agcgaactcg gaaggtaaaa
    10021 cgtgcacaat catctgtcaa ttcaatagga ctagtccgtt caaaaccgta aatctcaatt
    10081 tggcgttgag gacgctcaac ttcttcaccc gcccgtgcgt actcatagag cttgcggcca
    10141 tttactttga cggcggaata cataggtgga atctgggtaa tagtgccgac aaaactagcc
    10201 atagcttcat caactgcctg ctctgatata tccaaaaccg gtgtccgctc gacaaggtca
    10261 cccgacgcat cttcggtagt ggttgagtag ccgagggtaa tctctccctc gtaaatcttg
    10321 ccctcttcct gcatgtactc aatcatgcgc gtggccttgc cgactgcaat gggcagaaca
    10381 cctgtcacat ctgggtccaa ggtccccccg tgaccaatct tcttctcctg caaaatcttg
    10441 cggagcttaa aaacaacatc gtgcgaggtc atgcctgcct ctttttttac gttaataatt
    10501 cctgaaatca tgttcccatt ataacacaaa ggaccgctct tgtgggcggt ccggtatcaa
    10561 tcagtcatca tagaaacgat agctaccgaa tgttgtacct atcaaattct ctagttcctt
    10621 atgaatgcgt cgcatggttc cagaaggtac tgcatcgaat gttactgctt catcaatccc
    10681 ttcctcagcc tcttcatcat agacaatatg taaaga
//
LOCUS       DATUCG010000084        10713 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.086, whole genome shotgun sequence.
ACCESSION   DATUCG010000084 DATUCG010000000
VERSION     DATUCG010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10713
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.086"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            402..1091
                     /locus_tag="VCA20_001673"
     CDS             402..1091
                     /locus_tag="VCA20_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEP1835458.1"
                     /translation="MGKVIIGISGNEQEFPTKSGRTYVTVASELADGVRQAGGVPMVI
                     PMGTPDLAKDYINMIDKLILSGGQHVDPSLYGQERLIDSNDYLLERDEFELALIEEAL
                     RQGKPIFAVCRGMQLLNVALGGSLEQEVAHHWQDGIAGTSHRLQVKPKSRIGQLFAQG
                     SSINSFHQQRIKELAPGLVATGLDPRDGTIEAYESRGNQALFGIQWHPEFLYNDCKQH
                     RDLFQYLVDIF"
     gene            1243..2100
                     /locus_tag="VCA20_001674"
     CDS             1243..2100
                     /locus_tag="VCA20_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEP1835459.1"
                     /translation="MKLKKLFSLATAALSVGVLAACGNSSSSSSSDSSATTVRVGVMS
                     LSDSEQARWDKVQEILGDEVKLEFTQFTDYSQPNKAVAENEVDINAFQHYNFLNNWNQ
                     ENGEDLVAIADTYIAPIRLYSGTADGKNKYTKVEEIPDGAEIAVPNDPTNESRALYLL
                     QAAGLIKVGVSGTELATIADITENKKNLKITELDASQTASSLSSVDAAVVNNTFVLEA
                     GLDYKNALYKEQKDENSKQWYNLIAARSDWEKSEQAAAIKKIIEAYHTDEVKKVIEET
                     SDGMDEPVW"
     gene            2257..3624
                     /locus_tag="VCA20_001675"
     CDS             2257..3624
                     /locus_tag="VCA20_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEP1835460.1"
                     /translation="MADSKVQLFENDAIIQNYFEKLKVLISKKSIFAQQIGLLDVATY
                     LKDMFEEDGAEVVLDDSYAAPFVMATFKASVPDAKTLIFYNHYDTVPADADQVWEKGN
                     PFELTISDGYIYGRGVDDDKGHITARLSALKKYQARQDGQLPVNIIFIMEGAEESASV
                     DLDKYLSKYKEHLIGADLLVWEQGHRNSLHQLEIAGGNKGIVTFNLQVKSADLDIHSS
                     YGGVIDSASWYLLSALQSMRAADGRILVDGIYEQVQEPNERELALVEEFALATSQSVT
                     DIYGLTLPTLVEDRREFLKRLYFEPSITIEGLSTGYLGQGVKTIIPAQASAKMEVRLV
                     PGLEPYDVLDKIRQHLDKHGFEQVEVIFTLGEKSYRSDMSHPAIVNVIELAKKLTPEG
                     VAVLPTSPGTGPMHTVFHALGVPIAGFGLGNANSRDHAGDENVSIADYYSHVELVEEL
                     IASYE"
     gene            3617..4681
                     /locus_tag="VCA20_001676"
     CDS             3617..4681
                     /locus_tag="VCA20_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEP1835461.1"
                     /translation="MSKEMIKLDNIDVTFQQKKRTIEAVKDVTIHINQEDIYGIVGYS
                     GAGKSTLVRVINLLQVPTAGKITIDEDVIYEGEKVTLTPAQLRSKRREIGMIFQHFNL
                     MAQMTAEENVAFALKHSGLSKEEKKEKVAKLLDLVGLSDRAENYPAQLSGGQKQRVAI
                     ARALANDPKILISDESTSALDPKTTKQILALLQELNEKLGLTIVMITHEMQIVKDICN
                     RVAVMQDGRLIEEGSVLEIFSHPKEELTQDFIKTATGIDEALVKIYQQDIVKILPENS
                     ILVQLKYAGSNTDTAIVNDLYKFYQVSANILYGNIEILDHTPVGEMVVILSGEPGQLH
                     RAIEAVTEARVEVTILKGAN"
     gene            4682..5374
                     /locus_tag="VCA20_001677"
     CDS             4682..5374
                     /locus_tag="VCA20_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP1835462.1"
                     /translation="MLEWIQTNFPDIYKLGWDGQTGWLTHFNLTLYMTFVSFAFGGFM
                     GLVSGLFLVLTGPRGVIANKTAYWILDKVASIFRAIPFIILLAAIAPLTKIIVGKTIG
                     TEAALVPLALSVFPFFARQVEVVLSELDRGVIEAAQASGATFWDIVLVYLREGLPDLI
                     RVTTFALVSLVGYTAMAGAIGAGGLGQVALSYGYLRYNNDVTFLATLLILVIIFVIQF
                     IGDFLTRKISHR"
     gene            5559..6263
                     /locus_tag="VCA20_001678"
     CDS             5559..6263
                     /locus_tag="VCA20_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HEP1835463.1"
                     /translation="MSQFVVDSSFWSLFPDVKIGVLLLKGYKTPAQSPDELVKLLEES
                     NGIAQKFLTEDTFSENEVIRTYRQAYQKFKTKKGARSSIEALLKRSTSDRPVTTISPL
                     VDIYNAASLRFGLPCGAEDLDTFVGNLQLTVTEGGDEFYLIGDETNNPTLPGEVCYKD
                     DKGAVCRCFNWRDGERTMITDETKNAFLVMELVNSDRVEDLENALDFISQQAETFLGV
                     VPEKYLLDCDRPAIAL"
     gene            6571..7332
                     /locus_tag="VCA20_001679"
     CDS             6571..7332
                     /locus_tag="VCA20_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009659017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEP1835464.1"
                     /translation="MKNFYLVDSQEQLFNYQQFVSKYEAKWHDYIHFLRKEYQVRDLP
                     QALIWANEEAARKLLGQSPVPAYTNDIRMVMTPDLAVWKKIYQHQLDSYENSPDLQTL
                     SAHYKGLSENHLLQIIGHELAHWSDLFLYDFADYDANIWFEEGMVEYISRQYFLTEEE
                     FAQERFCNQLLVDLFQEKHGWHSLDAFGKSTYEGDYASIFYEYWRSFLTIDQLVEKVG
                     SVQALFETYHKWAQSDQSISLLNWFVQEELLEKEI"
     gene            7352..8269
                     /gene="glyQ"
                     /locus_tag="VCA20_001680"
     CDS             7352..8269
                     /gene="glyQ"
                     /locus_tag="VCA20_001680"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEP1835465.1"
                     /translation="MAKKLTFQEIILTLQQFWNEQGCLLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLESLER
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATGPVTAE
                     VTYGLERLASYIQEVDSVYDIEWADGVKYGEIFIQPEYEHSKYSFEVSDQDMLLENFT
                     KFEKEAERALEEGLVHPAFDCVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKKLGFPLLDEATRAELLKEDAE"
     gene            8269..10305
                     /locus_tag="VCA20_001681"
     CDS             8269..10305
                     /locus_tag="VCA20_001681"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEP1835466.1"
                     /translation="MTKNLLVELGLEEIPAYIVTPAMHQLRDRMATFLTDNRLAFDSI
                     DVFSTPRRLAVRVRGLADQQTDLTEDFKGPAKKIALDADGNFTKAAEGFVRGKGLTSA
                     DIEFREIKGEEYVYVTKHEAGQPAKAVLAGIPEVLKAMTFPVSMNWASNKFAYIRPVH
                     TLTVLLGDEALDMDFLDISSARISRGHRFLGNETEIASADSYEADLRAQFVITDPAER
                     QHMIVEQIKAIEDKHNVTVEIDEDLLNEVLNLVEYPSAFMGSFDTKYLEVPEEVLVTS
                     MKNHQRYFVVRDKSGKLLPNFISVRNGNDQYLDNVIKGNEKVLVARLEDGEFFWREDQ
                     KLKIADLVERLKVVTFHEKIGSLYEHMERTKVIAEKLADLAGLSADEKADVARAADIY
                     KFDLLTGMVGEFDELQGIMGEKYALLAGEKPAVAAAIREHYLPNSAEGELPESKVGAV
                     LALADKFDTLLSFFSVGLIPSGSNDPYALRRATQGIVRILEAFGWEIPLDQLIAELYS
                     LNFASLTYDNQPAVMDFIRARVEKMMDKAIPKDIREAVLASSTFVVRLQLVASSAIFQ
                     KAKEADYKEAVENLSRVFNLAEKAEVTAIDEALFENDQEKALAAAVAGLELTEDMAGN
                     LDKLFALSPVIAAFFDNTMVMVDDAAVKANRLALLKALADKASAVAVFNLLNSK"
     gene            10315..10572
                     /locus_tag="VCA20_001682"
     CDS             10315..10572
                     /locus_tag="VCA20_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002901318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEP1835467.1"
                     /translation="MEQAKIDRINELARKKKAEGLTEEELQEQAALREEYIEGYRRSV
                     RAHIEGIKVVDEDGNDVTPEKLRQVQREKGLHGRSLDDPNS"
BASE COUNT         3060 a         2030 c         2580 g         3043 t
ORIGIN      
        1 ttagctttta tttttttcaa acttttcctc caaaatattt ctatgcccct atcctaacag
       61 aaaaaacgac tcttgtataa ttgtaaaaaa aatagaggga gttataactt ttgactataa
      121 cagatttaaa gagagctact tttttggtat ttcttctata taaaagcttg aaaaaaacgc
      181 ctgtttatca agcttgactt ttttatgctg gctccagtat agttttttcc taagcgtgat
      241 atagatttta tctatgaaac tgccaaaggc aaactcttgt ttatgagcaa ttgtttatag
      301 gtagttatga tagttatttt ttatcaagcc ataaaacttc tatattagac agggctaggt
      361 agccatgcta caattgaaaa aatgaaagaa aggggctttt tatgggaaaa gttatcattg
      421 gaatttcagg aaacgagcaa gaatttccaa ctaaatctgg taggacttat gtaaccgttg
      481 cgagtgaatt ggcagatggt gtgcgtcagg caggtggtgt accaatggtt attcctatgg
      541 gaactcctga cttagccaag gactatatca acatgattga taagttgatt ttatcaggcg
      601 gtcagcatgt agacccaagt ctatacgggc aagagcgctt gattgatagt aatgattacc
      661 tcttggaacg ggatgaattt gaactggctt tgattgagga agcccttcgt caggggaagc
      721 ccatttttgc agtctgccga gggatgcagt tgctgaatgt tgctctaggc ggaagcttgg
      781 aacaagaggt ggctcatcat tggcaagatg gtattgcggg gacatcccat cgtttacaag
      841 tgaaacctaa aagccgaatc ggtcaactgt ttgcccaagg gagctctatt aattccttcc
      901 atcagcaacg aatcaaagaa ttggctccag gactggtggc gacaggtttg gatcctagag
      961 atggtacgat agaagcttat gaaagtagag ggaaccaagc tttgtttggt atccaatggc
     1021 atccagaatt tttatacaat gattgtaagc aacatcgaga cctgtttcag tatttagtag
     1081 atatatttta agaggggtat ccctcttttt attatcaata tttataattg caaaggtatt
     1141 tgttataacg aaaagttata actttctata aaaaaagtca attagtcaga aagaaaattc
     1201 tgtgttaaga tagatacagt tctaatttaa ggagatcatt ttatgaaatt aaaaaaattg
     1261 tttagtttgg caacagctgc tctatcagta ggtgtacttg ctgcatgtgg taactcgtct
     1321 tctagctctt catccgattc ttcagcaaca actgttcgtg tgggtgtgat gagcttgagc
     1381 gattctgagc aagcccgttg ggataaagtt caagaaatac ttggggatga agtgaaattg
     1441 gagttcactc aatttacaga ctactcacaa ccaaacaagg cagtagctga aaatgaagtg
     1501 gacatcaacg ccttccagca ctataacttc ttgaataact ggaatcaaga aaatggtgaa
     1561 gacttggttg ctattgcgga tacttatatc gcaccaatcc gtctttactc aggtacagct
     1621 gatggaaaaa acaagtacac taaagtggaa gaaattccag atggtgcaga aattgcggtt
     1681 ccaaacgacc caacaaacga aagtcgtgct ctttaccttc ttcaagcagc tggtttgatc
     1741 aaggttggtg tatctggtac agaattggca actattgctg atattactga aaacaagaaa
     1801 aacttgaaaa tcactgagtt ggatgcaagc caaacagcaa gctcacttag ctcagttgat
     1861 gcagcagttg taaacaacac atttgtgttg gaagctggtt tggattacaa aaatgccctt
     1921 tacaaagagc aaaaagatga aaattcaaaa cagtggtaca acttgattgc agctcgtagc
     1981 gattgggaaa aatcagagca agcagcagcg attaagaaaa ttatcgaagc ctaccacacg
     2041 gatgaagtga aaaaagttat tgaagagact tcagacggta tggatgagcc agtttggtaa
     2101 aatgtaaata ggttgaggac tgggcagaat tgttcagccc tctatctgtt tttgtagaga
     2161 taagaaaaaa tctcttaacc aaagtcagaa atgtgggaga atatttgatt ttggttagta
     2221 gatttaattg gttttcattg agtatgagga gaaagtatgg cagatagtaa agttcaatta
     2281 tttgaaaatg atgcgattat tcaaaactat tttgaaaagt tgaaggtctt gatttcaaag
     2341 aaatctattt ttgctcagca gattggtttg ttggatgtgg cgacttactt gaaagatatg
     2401 tttgaagaag atggtgcaga ggttgttttg gatgatagtt atgcagcacc ctttgtcatg
     2461 gctacgttta aggcttcggt gccagatgcg aaaacattga ttttttacaa tcactatgac
     2521 acggttcctg ctgatgccga tcaggtttgg gagaagggca accccttcga attgaccatt
     2581 tccgatggct atatctatgg tcgtggggtg gacgatgaca agggccatat cacagcccgt
     2641 ttatctgccc tgaaaaaata tcaggctaga caagatggtc agctccctgt taatatcatc
     2701 tttattatgg agggggcaga agagtcagct tctgtcgact tggacaaata cctttcaaaa
     2761 tataaagagc atttgattgg tgctgatttg cttgtctggg agcaggggca tcgcaatagc
     2821 ctacatcagc tagaaattgc tgggggaaat aaaggaattg tgaccttcaa tcttcaggtc
     2881 aaatcagctg acttggatat tcattcttct tatggaggag ttattgattc ggcaagctgg
     2941 tatttgttgt cagctcttca atccatgcgg gctgcagatg gacgaatttt ggttgatggt
     3001 atttacgaac aagtgcaaga accaaatgag cgtgaattgg ccttagtgga agaatttgca
     3061 cttgcgacca gtcagtctgt gacggatatt tatggactaa cccttccaac cttggtagaa
     3121 gatcgtcgtg agtttctgaa gcgtttgtat tttgagccat cgattaccat tgaaggcttg
     3181 tcaactggct atcttggtca aggtgtcaag accatcatac cagctcaagc ttctgctaag
     3241 atggaagtcc gtctggtacc aggcttagag ccgtatgatg ttttggacaa aattcgtcag
     3301 cacttagata agcatggttt tgaacaggta gaagttatct ttaccttggg tgaaaagagc
     3361 taccgaagtg atatgtcgca ccctgccatt gtcaatgtga ttgaattggc caaaaaattg
     3421 acaccagaag gagttgctgt cttgccgacg tcaccgggga caggacccat gcatacagtc
     3481 tttcatgctc taggtgtgcc gattgcagga tttggtctag gaaatgccaa cagtcgcgac
     3541 catgctggtg atgaaaatgt cagcatcgct gactactata gccatgttga attagtagag
     3601 gagttaattg caagttatga gtaaggaaat gattaagcta gataatattg atgtaacttt
     3661 ccagcagaaa aaacgtacga tcgaagcggt aaaggatgtg accatccata tcaatcaaga
     3721 ggatatttac ggtatcgtag ggtattctgg agcagggaaa tctacccttg ttcgagtgat
     3781 taatttattg caggtgccga cagcaggtaa aattactatc gatgaggatg taatttacga
     3841 gggagaaaag gtcacactta ccccagctca attgcggagc aaacgccgtg aaatcggcat
     3901 gattttccag catttcaatc tgatggctca aatgacggca gaagaaaacg tagcctttgc
     3961 tcttaagcat tcgggtttga gcaaggaaga gaagaaagaa aaagtcgcta agttgttgga
     4021 cttggttggg ctctctgacc gtgctgaaaa ctatccagct cagttgtctg gtggtcaaaa
     4081 gcaacgtgtt gccattgccc gtgccttggc aaatgaccct aaaatcttga tttctgatga
     4141 gtctacttca gccttggatc cgaagacgac caaacagatt ttggcacttt tgcaggaatt
     4201 gaatgaaaaa ttgggcttga ctattgtcat gattacccac gagatgcaga ttgtaaaaga
     4261 catttgtaac cgtgtggcgg ttatgcagga tggtcgcttg atcgaagaag gttcagtttt
     4321 agaaatcttc tctcatccaa aggaagaatt gacacaggat ttcatcaaaa cagcaacagg
     4381 aattgatgag gccctggtga aaatttacca gcaggatatt gttaagattt tgcctgagaa
     4441 cagtattttg gtgcaactca agtatgctgg ttccaatacg gatacggcaa ttgtcaatga
     4501 tttgtataaa ttctatcaag tatctgccaa tattctttat ggcaatattg aaattttgga
     4561 ccatacgcca gtcggtgaga tggtggtgat tttatcaggt gaacctggtc agttgcaccg
     4621 tgcgattgag gcagtgacgg aggcgcgtgt ggaagtgacg attttgaaag gagcaaatta
     4681 gatgttagaa tggattcaaa cgaattttcc agatatttat aaattgggct gggatggtca
     4741 gacaggttgg ttaacacatt ttaatttgac cctctatatg acctttgttt cctttgcttt
     4801 cggtggattt atgggcttgg tgtctggttt attcttggtt ttgacaggtc cacgtggggt
     4861 tattgctaat aagactgcct attggatttt ggacaaggtg gcttctatct tccgagccat
     4921 tccttttatt atcttgttgg cagctattgc tcctttgaca aaaattattg ttgggaaaac
     4981 gattggtaca gaggcggctt tggtgccgct cgccctttca gtctttccgt tctttgcacg
     5041 tcaggttgaa gtggtcttgt cagaattgga ccgtggtgtc attgaggctg ctcaggcatc
     5101 aggtgcgact ttctgggata ttgtccttgt ctatctgcgt gagggtctgc ctgacttgat
     5161 tcgtgtgacg acatttgccc tggtttcctt ggtcggctat acagccatgg caggggctat
     5221 cggagcaggt ggtttgggac aggttgctct atcatacggc tacttacggt ataataatga
     5281 tgtgaccttt ttagcaactc ttttaatttt agttatcatt ttcgtaatcc agtttattgg
     5341 agatttcttg acgagaaaga ttagtcatag atagttattt agctgtgttt tttcaaattt
     5401 tggatagtcc ttgacttatt taaagcattt ttatataata aatttgaata aataaacact
     5461 gccaccgggt agtcatgcaa cagcatttta tgaacgtcgt taggtcttag aagatgttca
     5521 tagggtgctg atttttctat aaaaaaagga gtgagaatat gtcacaattt gttgttgatt
     5581 catctttttg gagtctattt ccagatgtaa aaattggagt gttattattg aaggggtata
     5641 aaacgccggc acaatcacct gatgagttag tgaaattact ggaagaaagc aacggaattg
     5701 cccagaaatt tcttacggaa gataccttta gtgagaatga ggtcattcgt acatatcgtc
     5761 aagcctatca aaaatttaaa acaaaaaaag gcgcgcgatc tagtatcgaa gctcttttga
     5821 aacgttcaac tagtgaccgt ccagtcacaa caatttctcc attagtggac atttataatg
     5881 cggcgagttt acgttttggt cttccgtgtg gtgctgaaga tttagataca tttgtaggca
     5941 atctccaact aacggtcact gagggtgggg atgaattcta tctcattggt gatgaaacca
     6001 ataatccaac tctaccaggt gaagtttgct ataaggatga taaaggcgcc gtttgtcgct
     6061 gtttcaattg gcgcgatggt gagcgaacca tgattacgga tgagacaaag aatgctttct
     6121 tggtcatgga gttggtcaat tctgatagag tagaagattt ggaaaatgcc cttgacttca
     6181 tcagtcagca agcagagacc ttcttgggag tagtaccaga aaaatatcta ctagattgcg
     6241 ataggcctgc aatagccttg taggatttgt cagtgggatt gtgttcataa atagtggtct
     6301 ttctagacaa atgctgattt ttctgctaca atactaagca tgaaagaagg ttgtttcaaa
     6361 agcctgtcta gcgagcttgg gagagtgaaa gccaagccag tcttgaaata attggcgctt
     6421 tcccagtcgt gaccatgtcc gactcaatca gaatcataca aatgaagtaa taaattaggg
     6481 tggaaccgcg ttctcaacgc ccctatgtct gttgacaggg ggaagagaat gtggttcttt
     6541 ttctgtttta ccagagatga aagagagaat atgaagaatt tttatctagt ggatagccaa
     6601 gaacagctgt ttaactatca gcaatttgtc tcaaaatacg aggcaaaatg gcatgactat
     6661 atccattttc taaggaagga atatcaagtt cgtgatttgc cccaagccct tatctgggca
     6721 aatgaagaag ctgcaagaaa attacttggc caaagtcccg taccagccta cactaatgat
     6781 atccgcatgg tcatgacacc agacttagca gtttggaaaa agatttacca gcatcaacta
     6841 gatagctacg agaacagccc agacttgcag acattgagtg ctcactacaa aggtttgtcg
     6901 gaaaatcatc tgttgcaaat tatcggtcat gagctggcgc attggtcaga cctttttctg
     6961 tatgactttg cggactatga tgcaaacatc tggtttgaag agggcatggt cgagtacatc
     7021 agcagacagt attttttgac agaagaagag tttgctcaag agcggttctg taatcagctc
     7081 ttggttgacc tctttcaaga aaaacacggc tggcattcct tggatgcttt tggaaaatcc
     7141 acttacgaag gagattatgc gagtattttt tacgagtact ggcgaagttt tctcaccatt
     7201 gaccagttag tagagaaagt aggttcggtc caagcacttt ttgaaaccta tcataaatgg
     7261 gcgcagtcag accaaagcat ttcgctactc aactggtttg tgcaggaaga attattagaa
     7321 aaagaaattt aaaaagaaac aggagtaact tatggcaaaa aaacttacat ttcaggaaat
     7381 catcctgacc ttacaacaat tttggaatga gcaggggtgc ttgctcatgc aggcttacga
     7441 tacggaaaaa ggtgcgggga ctatgagccc ttacaccttc ttgcgtgcca tcggtcctga
     7501 accatggaat gcggcttatg tggagccttc acgtcgtcca gcggacggtc gttatgggga
     7561 aaatccaaac cgcctttatc agcaccacca attccaagtg gttatgaaac catctccgtc
     7621 taatatccaa gaactctact tggagtcttt ggagcgtttg ggcatcaatc ctttggaaca
     7681 tgatattcgt tttgttgagg acaactggga aaacccatca actggttcag ctggtctagg
     7741 ctgggaagtt tggttggacg gcatggaaat cacgcagttt acctactttc aacaggtcgg
     7801 cggcttggcg acaggtcctg tcacagctga agtaacctat ggtttggagc gtttggcttc
     7861 ctatatccaa gaggttgact ccgtttacga tatcgaatgg gctgacggtg ttaaatacgg
     7921 tgaaatcttc atccagccag agtatgagca ttcaaaatat tccttcgaag tatccgacca
     7981 agacatgctc cttgaaaact tcactaagtt tgagaaagaa gcagagcggg ctttggaaga
     8041 aggattggtt catccagcct ttgactgtgt actcaaatgt tctcatacct ttaacctgtt
     8101 ggatgcccgt ggtgccgtgt ctgtaactga gcgtgcaggc tacattgccc gtatccgtaa
     8161 tcttgcccgt gtcgttgcca agacctttgt ggcagagcgt aagaaactgg gctttccact
     8221 tttagatgaa gccacacgag cagaattgtt gaaggaggac gcagaataat gacaaagaat
     8281 ttacttgttg aacttggctt ggaagaaatt cctgcctaca tcgtaacccc agccatgcac
     8341 cagttgcgtg accgcatggc gacctttttg acagacaatc gcttggcttt tgacagcatt
     8401 gatgtctttt caacaccacg ccgtttggct gttcgtgtgc gtggtttggc agaccagcag
     8461 actgatctta cagaagattt caaaggtcct gctaagaaga ttgccttgga tgcagacgga
     8521 aacttcacta aagctgcaga aggctttgtc cgtggcaaag gcttgacaag tgctgacatc
     8581 gaattccgcg agattaaggg tgaagagtat gtctacgtga ccaaacacga agctggccag
     8641 ccagctaagg cagtcttggc aggcattcca gaggtcttga aggctatgac cttcccagtc
     8701 agcatgaact gggcatccaa caaattcgcc tacatccgcc ctgtccacac cttgaccgtt
     8761 ctcttgggtg acgaggcatt ggatatggat ttcttggaca tttcatctgc tcgcatcagc
     8821 cgtggtcatc gtttccttgg aaatgaaact gagattgcaa gtgcagattc ttacgaggct
     8881 gacttgcgtg cacagtttgt tatcactgat cccgcagagc gtcaacatat gattgtcgaa
     8941 caaatcaagg ctatcgagga caaacacaat gtgaccgttg agatagatga ggacctgctc
     9001 aatgaagttc tcaacttggt cgagtaccca agtgccttta tgggttcctt tgacaccaaa
     9061 tacttggaag taccagaaga agtcttggta acttccatga aaaatcacca acgttacttc
     9121 gtggtgcgtg ataagtctgg taaactcttg ccaaacttca tctccgtccg taacgggaat
     9181 gaccagtacc ttgacaatgt catcaaaggg aatgaaaaag tcttggtggc acgtttggaa
     9241 gacggcgaat tcttctggcg tgaagaccaa aaactcaaga ttgctgactt ggtggaacgc
     9301 ctcaaagtcg tgaccttcca tgaaaaaatt ggctcacttt acgagcacat ggaacgcacc
     9361 aaagttatcg cagaaaaact ggctgacttg gctggcttgt ctgcggatga gaaagcagat
     9421 gtggcacgtg cagcggacat ctacaagttt gacctcttga caggcatggt cggcgagttt
     9481 gatgaattgc aagggattat gggtgagaaa tacgctcttc ttgcagggga aaaacctgcg
     9541 gtggcagcag caatccgtga gcactacttg ccaaactcag cagaaggcga attgcctgag
     9601 agcaaggtcg gtgcggtctt ggcactggct gataaattcg acactctcct atccttcttc
     9661 tctgttggtt tgattccttc tggctcaaac gacccttacg ctcttcgtcg tgcaacacag
     9721 ggtatcgtgc ggattttgga agcatttggt tgggaaattc cattggatca gttgattgcg
     9781 gagctctaca gcttgaactt tgccagcttg acctatgaca accagccagc tgtgatggac
     9841 tttatccgtg cccgtgtaga gaagatgatg gataaggcga ttccgaaaga catccgtgag
     9901 gctgtgctgg ctagctcaac cttcgtggtc agactgcaac tggtagccag ctctgctatt
     9961 ttccaaaaag caaaagaggc tgactacaag gaagccgtgg aaaacttgtc acgtgtcttc
    10021 aacttggctg aaaaggcgga ggtgactgcg attgacgagg ccctcttcga aaacgaccag
    10081 gaaaaagccc ttgctgctgc agtggcaggc ttggagttga cagaagatat ggcgggcaac
    10141 ttggacaagc tctttgctct cagcccagtc atcgcggcct tcttcgacaa taccatggtt
    10201 atggtagatg acgcagcagt caaagccaac cgcttggctc tcttgaaagc cttggcggat
    10261 aaggcaagtg ctgtggcggt cttcaatctc ttgaatagca agtaggaggc ggacatggaa
    10321 caagcaaaaa ttgatcgtat caatgaattg gcccgcaaga aaaaggcgga aggcttgaca
    10381 gaggaggagt tgcaggaaca agcagctctt cgtgaggagt acatcgaggg ctaccgccgt
    10441 agtgtgcgtg cccacatcga aggtatcaag gtcgtcgatg aagacggcaa tgatgtgaca
    10501 cctgagaaac tccgtcaagt ccaacgtgaa aaaggcttgc atgggcgtag tttggatgat
    10561 ccaaattcct aagaataaat gataataaac aaggaaagac taggttttct agtctttttt
    10621 actcttgcag cctacttaca ataacgaatt actcgagata tagacaatat gtaaagaccc
    10681 ccagttgaga attcgtggat ttacctgtac act
//
LOCUS       DATUCG010000085        10677 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.087, whole genome shotgun sequence.
ACCESSION   DATUCG010000085 DATUCG010000000
VERSION     DATUCG010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10677
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.087"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(4..408)
                     /locus_tag="VCA20_001683"
     CDS             complement(4..408)
                     /locus_tag="VCA20_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEP1835468.1"
                     /translation="MAGRKISPQSLKNLYQSNKEANQLTKESIETALLFLLEKKELKQ
                     ISVSELVRKAGVSRNAFYRNYKSKEEILEDYYERTSSNLKKKWHDLQDKVQKDGLKQS
                     FTDFVQEQKRKAEQSKTLSNVSQWIKEKTKRD"
     gene            535..1386
                     /locus_tag="VCA20_001684"
     CDS             535..1386
                     /locus_tag="VCA20_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEP1835469.1"
                     /translation="MSKWKIVADSGCDYRQLANLAPDTEFISVPLTIQVGEQAFVDDA
                     SLDIDHMMEVMEASKSAAGSACPSPQAYQAAFEGAENIIVVTITGGLSGSFNAARVAR
                     DMYIEEHPNVNIHLIDSLSASGEMDLLVDEINRLIGAGLDFPQVVEAITHYREHSKLL
                     FVLAKVDNLVKNGRLSKLVGTVVGLLNIRMVGEASAEGKLELLQKARGHKKSVTAAFE
                     EMKKAGYDGGRIVMAHRNNAKFFQQFSELVKASFPTAVIDEVATSGLCSFYAEEGGLL
                     MGYEVKA"
     gene            complement(1485..2744)
                     /locus_tag="VCA20_001685"
     CDS             complement(1485..2744)
                     /locus_tag="VCA20_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEP1835470.1"
                     /translation="MKKYQVIIQDILTGIEEHRFKRGEKLPSIRQLSEQYHCSKDTVQ
                     KAMLELKYQNKIYAVEKSGYYILEDRDFQDHTVELNPADFQELPYEDFRICLNESLIG
                     RENYLFNYYHQQEGLAELISSVQSLLMNYHVYTKKDQLVITAGSQQALYILTQMETLA
                     GKTEILIENPTYSRMIEIIRHQGIPYQTIERDLDGIDLEELESIFQTGKIKFFYTIPR
                     LHNPLGSTYDIATKTTIVKLAKQYDVYIIEDDYLADFDSSHSLPLHYLDTDNHVIYIK
                     SFTPTLFPALRIGAISLPNQLRDTFIKHKSLIDYDTNLIMQKALSLYIDNGMFARNTQ
                     HLHHIYHAQWNKIKDCLETYALNIPYRISKGSVTFQLSKGILSPSIQHMFEKSYYFSG
                     QKADFLQIFFEQDFADKLEQFVRYLKE"
     gene            2867..3601
                     /gene="yaaA"
                     /locus_tag="VCA20_001686"
     CDS             2867..3601
                     /gene="yaaA"
                     /locus_tag="VCA20_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEP1835471.1"
                     /translation="MKIIIPNAKEVNTNLENASFYPLPDRSKPVLDAISQFDVKKMAA
                     FYKLNEAKAELEADRWYRIKTGQAKTYPAWQLYDGLMYRYMNRRDIDSKEENYLRDHV
                     RVATALYGLIHPFEFISPHRLDFQGSLKIGNQSLKQYWRPYYDQEVGDDELIFSLASS
                     EFEQVFSPQIQKRLVKILFMEEKAGQLKVHSTISKKGRGRLLSWLAKNNIQELSDIQD
                     FKVDGFEYCTGKSTENQLTFIRSVKI"
     gene            3731..5170
                     /locus_tag="VCA20_001687"
     CDS             3731..5170
                     /locus_tag="VCA20_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEP1835472.1"
                     /translation="MKKRSRWRKSPKLKLVNFALWVLYAIILCLFLVTMYRYNILDFR
                     YLNYIVTILLIGVAVLTGLLMWRKKARIFTALVLIFSLVITSVGIYGMQEVVKFSTRL
                     NSNSTFSEYEMSILVPANSEITDVRQVTNVLAPAEYDQDNITALLNDISKMESTQLTT
                     SPTTSYLTAYQAMLNGESQAMIFNGVFTNILENEDPDFSSKVRKIYSFKVTQTVDTAT
                     KQVSGDSFNIYISGIDTYGPISSVSRSDVNIIMTINRATHKILLTTTPRDSYVAIADG
                     GQNQYDKLTHAGIYGVNASVHTLENLYGINISNYIRLNFTSFLQLIDLVGGIDVENTQ
                     EFTSGEHTFPVGTVHLDAEQALIFVRERYSLANGDNDRGKNQEKVIAALIKKLSSPEN
                     LRNYQAILNSLEGSIQTDISIETIIGLVNTQLEAGTQFTVESQALVGNGRTDLPSYAM
                     PDSQLYMLEINQESLEQTKAAIQSVLDGN"
     gene            5188..5877
                     /locus_tag="VCA20_001688"
     CDS             5188..5877
                     /locus_tag="VCA20_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein CpsC"
                     /protein_id="HEP1835473.1"
                     /translation="MNNQEMNTIEIDILLLLKTIWRKKIIILLLGLLGAGLAFAYSSF
                     LVTPQYDSTTRIYVVGQHAEAGVGVTNQDLQAGTYLVKDYKEIILSQDVLTQVATELN
                     LNGNLKEKVSVSIPVDTRIVSISVRDSNPNEAARIANSLRAFASQKIIEVTKVSDVTT
                     LEEAVPAEEPSTPNTKRNILLGLLAGSILATGLVLVMEILDDRVKRPQDIEEVMGLTL
                     LGVVPDSKKLK"
     gene            5887..6582
                     /locus_tag="VCA20_001689"
     CDS             5887..6582
                     /locus_tag="VCA20_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HEP1835474.1"
                     /translation="MATLEIARTKKELVNKTEEYFNAIRTNIQLSGEDIKVVGITSVQ
                     SNEGKSTTAASLAIAYARSGYKTVLVDADIRNSVMSGFFKPITKITGLTDYLAGTTDL
                     SRGLCETNIPNLTVIESGKISPNPTALLQSKNFENLLATLRRYFDYVIVDCPPLGVVI
                     DAAIIAQKCDAMVLVAEAGNVKRSAVKKIKEQLEQTGTPFLGVILNKYDITTEKYGDY
                     GNYGNYGNYGKKA"
     gene            6620..7351
                     /locus_tag="VCA20_001690"
     CDS             6620..7351
                     /locus_tag="VCA20_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HEP1835475.1"
                     /translation="MIDVHSHIIFGVDDGPKTIEESLDLIGEAYHQGVRFIVATSHRR
                     KGMFETPEKIIMSNFVQVKERVKVAYPEMTLCYGAELYYNKDILSKLEKKEVPTLNGT
                     RYILLEFSKNTPWKEIQEAVNEVMLLGLTPLLAHIERYDALAFQSERVEELIDKGCYT
                     QINSNHVLKLGLIGDRAKEFKKRARYFLEQDLVHCVASDMHNLIARPPFMRDAYKLIK
                     KEFGEDRAKALLKKNPLMLLKNQVI"
     gene            7380..9206
                     /locus_tag="VCA20_001691"
     CDS             7380..9206
                     /locus_tag="VCA20_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEP1835476.1"
                     /translation="MDLGTVTDKLLERNSKRLILICMDTFLIVVSMNLSRLFLDIIID
                     IPDKRFILAVLFVLIFYLIISVRLKVFSLITRYTGYQSYVKIGLSLISTYSLFLIISM
                     ILWRTFSYRFILVSLFLSYVMLITPRIVWKVLHETRKNAIRKKDSPLRILVVGAGDGG
                     NIFINTVEDRKLNFEIVGIVDRDPNKLGTFIRTAKVLGNRNDIPRLVEELAVDQVTIA
                     IPSLNGKEREKIVEICNITGVTVNNMPSIEDIMAGNMSVSAFQEIDVADLLGRPEVVL
                     DQEELNQFFKGKTILVTGAGGSIGSELCRQIAKFTPKRLLLLGHGENSIYLIHRELLE
                     KYQGKIELVPLIADIQDRELIFSIMAEYQPDVVYHAAAHKHVPLMEYNPHEAVKNNIF
                     GTKNVAEAAKVARVEKFVMVSTDKAVNPPNVMGATKRVAEMIVTGLNEPGQTQFAAVR
                     FGNVLGSRGSVVPLFKEQIKKGGPVTVTDFRMTRYFMTIPEASRLVIQAGHLAKGGEI
                     FVLDMGEPVQILELARKVILLSGHTEEEIGIVESGIRPGEKLYEELLSTEERVSEQIH
                     EKIFVGRVTNKQSDIVNSFINGLLQKDRNELKDMLIEFAKQE"
     gene            9546..10652
                     /locus_tag="VCA20_001692"
     CDS             9546..10652
                     /locus_tag="VCA20_001692"
                     /inference="COORDINATES: protein motif:HMM:NF013884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEP1835477.1"
                     /translation="MKRKVVAQKDKAIKNNGSERQSNIELLRIVSMLMIVSHHFSVHG
                     GFEFTAQDITLNRFWLLFLAMWGKIGVNLFIFISGYFLSANNKLNLNKILKTWLQVFS
                     YSVSILIVVYFIEGSKLSIKEGVAFLFPITFSLWWFASSYLFLLLFSPFINRLIFQLT
                     KIEHRNIILVLGVFWSIWTTLTNTNMQSNSLLWFIYLYIIAAYIRTYQQDILQYGKKI
                     VTASMLMLALTYMSGIVLVIAGLWIPLFVTRAYYLFRGQSILILIASVLIFLCFLRLN
                     MKSIKWINIIATSSFGIYLIHDCIFIRMYLWQKLFRNASYFSSPYLIPYSIFAISAVF
                     IGSCLCSLLMNKMLDGPIEKVNRWLLACTFIKNK"
BASE COUNT         3354 a         1653 c         2268 g         3402 t
ORIGIN      
        1 cttttaatcc cgttttgttt tttccttgat ccattgactg acattggaca gggttttgct
       61 ttgctctgcc tttcgttttt gttcctggac aaaatctgta aagctttgct tgagaccgtc
      121 cttttgaact ttgtcctgca aatcatgcca tttcttttta aggttgctag aagtccgttc
      181 gtagtagtct tcaaggattt cttccttgga cttgtaattg cggtagaagg cattgcgtga
      241 cacaccagct tttcggacca attcagatac cgaaatttgc ttgagctcct ttttctctag
      301 gagaaatagc agagcagttt ctattgattc cttagtcagt tgattcgctt ctttatttga
      361 ttgatagagg tttttcaaag attggggtga aattttacga cctgccatag ttttcctttt
      421 ccgagtgtca catctgaaag aaaatgcttt cagatagggt agtttaatga tataattgta
      481 agtaaataga gatttattgt caaataaaaa agtaaaattg gaaatgagaa tattatgtca
      541 aaatggaaaa ttgttgcaga ttcgggctgc gactatcgtc aattagctaa tctagctccg
      601 gacactgaat ttattagtgt gccactcacc atccaagttg gagaacaagc atttgttgat
      661 gatgcaagct tggatattga tcacatgatg gaggtgatgg aagcatctaa gtctgcagcg
      721 gggtctgcgt gtccaagtcc gcaggcttat caggcagctt ttgagggagc tgagaacatt
      781 atcgttgtga cgattacagg tgggctatcg ggtagtttta atgcggcacg tgtagctagg
      841 gatatgtata tcgaagagca tccgaatgtc aatatccatt tgatagatag tttgtcagcc
      901 agtggggaaa tggatttact tgttgacgaa atcaatcgct taattggtgc aggactagat
      961 tttccacaag tagtagaagc gataactcac tatcgggaac acagtaagct cctctttgtt
     1021 ttagctaagg ttgataatct tgttaagaat ggaagactga gcaaattggt aggcactgtc
     1081 gttggtcttc tcaatattcg tatggttggt gaggcaagcg ctgaaggaaa attagagttg
     1141 cttcaaaagg cgcgtggtca taagaaatct gtgacagcag cctttgaaga aatgaaaaaa
     1201 gcaggctatg atggtggtcg aattgtcatg gcccaccgca acaatgctaa gtttttccaa
     1261 caattctcag agttggtaaa agcaagtttt ccaacggctg ttattgacga agttgcaaca
     1321 tcaggtctat gcagttttta tgctgaagaa ggtggacttt tgatgggcta tgaagtgaaa
     1381 gcgtgattca cagagtaaca attttgggat gtagtttcca ctatagaata atccccctcg
     1441 tcttctaagt tcgaggggga ttgtttgtat gagactattg gatttcattc tttcaaatat
     1501 cttacgaatt gctccagttt atctgcaaaa tcttgttcaa agaagatctg taagaaatca
     1561 gctttctgtc cgctgaaata ataagacttt tcaaacatat gttggatgct aggagaaaga
     1621 atccccttgc ttagctgaaa ggtcacgctc cctttggaaa ttcgatacgg gatgttcaaa
     1681 gcgtatgtct ctaaacaatc ttttatttta ttccactgag cgtgataaat gtgatgaaga
     1741 tgctgtgtgt tccgtgcaaa cataccgtta tcaatgtaga gcgagagagc tttttgcatg
     1801 ataagattgg tatcgtagtc gattagactc ttatgtttga tgaaggtatc acgtagctga
     1861 ttaggaaggc tgattgcacc gattcggagg gcaggaaaga gtgtcggtgt aaaagatttt
     1921 atatagatga catgattatc tgtatcaaga tagtgtaaag gtaggctatg actagagtcg
     1981 aaatctgcta aatagtcatc ctcaatgatg tagacatcgt attgctttgc taattttacg
     2041 atggttgttt ttgttgctat atcataagtt gaaccgagag ggttgtgcaa gcgaggaatt
     2101 gtgtagaaaa acttaatttt tccagtttgg aagatacttt ccaattcttc taggtcaatt
     2161 ccatctaaat cccgttcaat tgtttgatag gggattcctt gatgtcgaat gatctctatc
     2221 attcgtgaat aggtagggtt ctctatcaag atttccgttt ttccagccaa ggtttccatt
     2281 tgtgtgagaa tatatagggc ctgttgacta ccagctgtga taaccagttg gtcttttttt
     2341 gtatagacat gatagttcat taacagactt tgaacggagg aaatcaattc cgctaatccc
     2401 tcttgctggt gatagtagtt gaataggtaa ttttcccgcc caataagact ttcgtttaga
     2461 caaatccgaa aatcttcata gggcaattct tgaaagtctg caggattgag ctctacagta
     2521 tgatcttgga aatctctatc ttctaagata taataaccgc ttttttcgac agcgtagatc
     2581 ttattttggt attttaattc caacatagcc ttttggacag tgtctttgct acaatgatat
     2641 tgctcgctga gttgacggat agaaggtaat ttctctccac gtttgaatcg atgttcctct
     2701 attccagtca aaatatcttg gatgataact tgatattttt tcatctaggc cccctttttt
     2761 atagactatg ttactagcta gtatatagga aaaattgaag aaagacaata tatgaataat
     2821 ggggttgagg ttcaggaatt aagctactct atggtataat taagtgatga aaataattat
     2881 acctaatgca aaagaagtaa atacaaatct agagaatgcc tcgttttatc ccctgcctga
     2941 tcgaagtaag ccggtgctgg atgccataag tcaatttgat gtaaaaaaga tggctgcctt
     3001 ttataaattg aatgaagcaa aggctgagtt agaagctgac cgttggtatc gaatcaagac
     3061 aggtcaagca aaaacctatc cagcctggca gttatatgat ggtctcatgt atcgttatat
     3121 gaataggcga gatatagatt cgaaagaaga aaattaccta cgtgaccatg ttcgtgtagc
     3181 gacagcctta tacggattga ttcatccttt tgaattcatt tcacctcacc gcttagattt
     3241 tcaagggagc ttaaagatag gcaatcagtc tttgaaacag tactggcgac cgtattatga
     3301 ccaagaagtt ggtgatgatg aactgatttt ctcactggct tcgtcagaat ttgagcaggt
     3361 gttttctcca cagattcaga aaagattagt taaaattctt ttcatggaag aaaaagcagg
     3421 tcagctaaaa gttcactcga ctatatcaaa aaaaggcaga ggaagattgc tgtcctggtt
     3481 ggctaagaac aatattcagg aattatcgga cattcaagat tttaaggtgg atggctttga
     3541 atattgtact ggaaaatcaa cagagaatca gctgactttc atacgctcag ttaaaatatg
     3601 aaaaaaagaa caagatatga acaagattga acaagataac gtttttcatc actaaatatg
     3661 gctatgtggc tagtaaaata ttgttttttg tgatacaata ggcgagttat aagtaaaagg
     3721 aaggttgttt atgaagaaga gaagcagatg gaggaagtcg cctaagctta agttggtaaa
     3781 ttttgcgctt tgggtgctat atgccattat tctatgtctg ttcttagtga ctatgtatcg
     3841 ctataacatc ctagattttc ggtatttgaa ctatattgta acaatcctgt tgataggggt
     3901 tgcagtattg actggcttgt tgatgtggcg taagaaagca cgcatattta cagcactcgt
     3961 acttattttt tcactggtca tcacttctgt cggaatctat ggaatgcaag aagttgtaaa
     4021 attttcaaca cgactaaatt caaattcgac attttcagaa tatgaaatga gcatcctcgt
     4081 cccagcaaat agtgagatta cagatgttcg acaggtcact aatgtacttg ctccggctga
     4141 atacgatcaa gacaacatca ctgctttatt aaatgatatt tccaaaatgg agtctactca
     4201 attaactaca agtccgacga cctcttatct aacagcctac caagctatgc taaatggtga
     4261 gagtcaagca atgattttca atggagtttt taccaatatt ttagaaaatg aggatccaga
     4321 cttctcttca aaagtgagga aaatatatag tttcaaagtg actcagactg ttgatacagc
     4381 tactaagcag gtgagtggag acagttttaa tatctatatc agcggcattg atacatatgg
     4441 tccaatttcg tctgtttcgc gttcagatgt taatattatt atgactatta atcgtgctac
     4501 acataagatt ttattgacaa ctactccacg agattcttat gttgctatcg cagatggtgg
     4561 acaaaatcaa tacgacaaat taactcatgc tggtatttat ggtgtcaatg cctctgtgca
     4621 taccttggaa aatctttatg gtatcaatat cagtaattat atccgtctca actttacatc
     4681 cttcctacag ttgattgatt tggttggagg tatcgatgta gaaaacaccc aagagttcac
     4741 aagtggtgag catactttcc cagtgggaac agtacactta gatgcagagc aagcgctaat
     4801 ctttgttcgt gagcggtatt cgttggctaa tggtgataat gaccgtggta aaaatcaaga
     4861 aaaagtcatt gcagcactta taaaaaaatt gagctcacca gagaatttac gtaattatca
     4921 agctatttta aatagtttag aaggatctat tcaaacggat ataagtattg aaacaattat
     4981 aggcttagtt aatacgcaat tagaagccgg cacacagttt acagtggagt cacaagcgct
     5041 agttggaaat ggtcgaacag acttgccttc ttatgctatg ccggattcac aactttatat
     5101 gttggaaata aaccaagaaa gtttggaaca aacaaaggcc gctattcagt cagtattgga
     5161 tggaaattaa aaaaataggg agaaaacatg aataatcaag aaatgaatac aatcgaaatt
     5221 gatattttac tcttactgaa aacgatttgg agaaaaaaaa tcattatcct tctgctggga
     5281 ctattaggtg cagggttagc ttttgcttat agtagttttt tggtaacgcc gcaatatgat
     5341 tcgacaactc gtatctatgt agttgggcaa catgctgaag ctggtgtagg agtgactaac
     5401 caggaccttc aggcaggaac ttatcttgta aaagattata aggaaattat cctatcacaa
     5461 gatgtactga cacaggtggc tacagagttg aatttgaatg gaaacctaaa agaaaaggtt
     5521 tcggtttcta ttcctgttga tactcgtatt gtttctatct ccgtgcgtga ttcgaacccg
     5581 aatgaggctg ctcgcattgc aaatagtctc cgtgctttcg catcgcaaaa aatcatcgaa
     5641 gttacaaaag tgagcgatgt tacaacactc gaggaggctg tcccagcaga ggagccatca
     5701 actcctaata ccaaacgcaa tatcttgctt ggtttattag ccggcagtat cttggcaacg
     5761 ggtctcgtgt tggttatgga aattttggat gaccgtgtaa aacgtcctca ggatatcgaa
     5821 gaggtaatgg gattgacctt acttggtgta gtgccagatt cgaagaaatt gaaataggag
     5881 aaaaatatgg cgacattaga aattgcacgt acgaagaaag aattagtaaa taagacagaa
     5941 gaatatttta acgctatccg tacgaatatt caactcagtg gggaagatat taaagtggtt
     6001 ggcataactt ctgtccaatc aaatgaagga aagagtacaa cagcagccag tcttgctatt
     6061 gcctatgctc gttctggcta caagacagtc ctggttgatg ccgatattcg aaactcagtg
     6121 atgtcaggtt tctttaagcc aattacgaaa attactggtt taactgatta tttagcagga
     6181 actactgatt tatctcgagg gctatgtgaa acaaatattc cgaatttaac agttatcgag
     6241 tctggtaaaa tatcaccaaa cccaactgcc cttttgcaaa gtaagaattt tgagaactta
     6301 ttagctactc ttcgtcggta ttttgactat gtgattgttg actgcccccc tctaggggtg
     6361 gtaattgatg ctgcaattat tgctcagaag tgtgatgcaa tggttttagt agcagaggca
     6421 ggaaatgtca aacgttcagc tgtgaaaaaa ataaaagaac agctggaaca aacaggaaca
     6481 ccgttcttag gcgtcatttt gaacaagtat gatattacaa ctgagaaata tggtgattat
     6541 ggaaattatg gaaattatgg taattacggt aaaaaagctt gatttttatt atatgagctt
     6601 tgattaagta ggtgttagta tgattgatgt tcattcgcat attatatttg gtgtggatga
     6661 tggtccaaag acaattgaag agagtctaga tttgatagga gaagcttatc accaaggggt
     6721 tcgcttcata gttgctactt ctcatagacg aaaagggatg tttgaaactc ctgagaaaat
     6781 tattatgtct aattttgttc aagtaaaaga gagagttaaa gtagcttatc cagaaatgac
     6841 tttatgttat ggagcggaac tttactataa taaagatatc ctaagtaaac tcgaaaaaaa
     6901 ggaagttcca actcttaatg gaacgcgcta tattctccta gaatttagta agaatactcc
     6961 ttggaaagaa atccaagagg ctgtgaatga agtaatgtta cttggactga ctccactact
     7021 tgcccatata gagcgttatg atgctctggc atttcagtca gagcgggtag aagagttaat
     7081 cgacaagggg tgttatactc aaattaatag caaccatgtg ttaaaactag gtttaattgg
     7141 tgatcgagca aaagagttta agaaacgggc acggtacttt ttagagcagg atttggtgca
     7201 ctgtgtggct agtgatatgc ataatttgat agcccgacct ccttttatga gggatgctta
     7261 taagctaatt aaaaaggaat ttggggaaga tagagcaaaa gctttattga aaaagaaccc
     7321 tcttatgtta ttaaaaaacc aggtgattta agctggttac tctagatttt ggagaaaaaa
     7381 tggatttagg aactgttacc gataaactgt tagagcgtaa tagcaaaaga ttgatactta
     7441 tatgcatgga tacttttctt attgtagttt ccatgaattt gagtagactg ttcttggata
     7501 ttattattga cattccagat aaacgtttca ttcttgcagt tttatttgta ttgatatttt
     7561 atctgattat ttcagttaga ttaaaggttt tttcgttaat tacgaggtat actggttatc
     7621 agagttatgt aaaaataggg ttgagcttga tatctactta ttcattattt ttaatcattt
     7681 caatgattct atggcggacc tttagttatc gtttcatctt agtatcctta tttttgtcgt
     7741 atgtaatgct cattactccg aggattgttt ggaaagtctt acatgagacg agaaaaaatg
     7801 ctattcgaaa gaaggatagc ccactaagaa tcttagtagt aggtgctgga gatggtggga
     7861 atatttttat caataccgtc gaagatagaa aattgaattt tgaaattgtc ggtatagttg
     7921 atcgtgatcc aaataaactt ggaacattca tccgtactgc caaagtttta ggaaaccgta
     7981 atgatattcc acgattggta gaagaattag ctgttgacca agtgacgatt gccatccctt
     8041 ctttaaatgg taaggagaga gagaagattg ttgaaatctg taacattaca ggagtgaccg
     8101 tcaataatat gccgagcatt gaagacatta tggcggggaa catgtctgtc agtgcctttc
     8161 aggaaattga cgtagcagat cttcttggtc gaccagaggt tgttttggat caggaagagt
     8221 tgaatcagtt tttcaaaggg aaaacaattc ttgtcacagg agcaggcggg tcgattggct
     8281 cagagttatg tcgtcaaatt gctaagttta cgcctaaacg cttgttgttg cttggacatg
     8341 gagaaaattc aatctatctc attcatcgag aattactgga aaagtaccaa ggtaagattg
     8401 agttggtccc tctcattgca gatattcaag atagagaatt gatttttagc ataatggctg
     8461 aatatcaacc cgatgttgtt tatcatgctg cagcacataa gcatgttcct ttgatggaat
     8521 ataatccaca tgaagcagtg aagaataata tttttggaac gaagaatgtg gctgaagcgg
     8581 ctaaagtagc tagggttgaa aaatttgtaa tggtttcgac cgataaggca gtcaatccac
     8641 caaatgttat gggggcgact aagcgggtag cagaaatgat tgttacaggt ttaaacgagc
     8701 caggtcagac tcaatttgcg gcagtccgtt ttgggaatgt tctaggtagt cgtggaagtg
     8761 ttgttccgct attcaaagag caaataaaaa aaggtggacc cgttacggtt acagacttta
     8821 ggatgactcg ttatttcatg acaatcccag aagcaagtcg tttggttatt caagcaggac
     8881 atttggcaaa aggcggagaa atctttgtct tggatatggg tgagcctgta caaattctgg
     8941 aattggcaag aaaagttatt ttgctgagtg gtcatacaga ggaagaaatc gggattgtag
     9001 aatctggaat cagaccaggc gagaaactct atgaggaatt gttatcaaca gaagaacgtg
     9061 tcagcgaaca gattcatgaa aaaatatttg tgggtcgcgt tacaaataag cagtcggaca
     9121 ttgtcaattc atttattaat ggattactcc aaaaagatag aaatgaatta aaagatatgt
     9181 tgattgaatt tgcaaaacaa gaataagaaa gtaaaaaata tttttacttt cctagagtta
     9241 aatgctattt agaacctatg tccataagaa agcaatatga tatagttcaa cagatagtga
     9301 gtatgcaagg cttgaaggat gatatctgaa agaattgtac ctataaccga cataaatgtg
     9361 agtaggtaaa agcaacttta tacattacta aaacaaggtg ccagagatat atctcaaaag
     9421 aggacttttg tatagtatac cttactggaa aatttttagc tcctattgtt aattatgaag
     9481 gaggaatatt tatatatttg aggagaaaaa gaagtttcta tatttatgat gtgaaggaaa
     9541 ttaatatgaa aaggaaagta gtagcccaaa aggataaggc tataaaaaat aatggaagtg
     9601 aaagacagtc caatattgag ttgttgagaa tagtatcaat gctaatgatt gtttcccatc
     9661 atttttcagt tcatggtgga tttgaattta cagctcaaga tataacactt aatagatttt
     9721 ggttgttatt tttagctatg tgggggaaaa taggtgttaa tctgtttatt tttatttcag
     9781 gttacttcct ttctgccaac aataaactta atttgaataa gatattgaaa acatggcttc
     9841 aagtattttc ttattcagta tcaatactta ttgttgttta ctttatagaa gggtcaaaac
     9901 taagtattaa agagggagta gcctttctat ttccaataac attttcactc tggtggtttg
     9961 ctagttctta tctattttta ctgcttttct caccatttat aaacagacta atattccaat
    10021 taactaaaat tgaacacaga aatataatct tggttttggg tgttttttgg agtatatgga
    10081 ctaccttgac aaatacaaat atgcaaagta actctcttct atggtttatt tatctttata
    10141 tcattgcagc ttatataaga acatatcaac aagatattct ccaatatggt aaaaaaattg
    10201 tgactgccag tatgttgatg cttgcgctaa cctatatgtc gggcattgtt ttagtgattg
    10261 ctggtctctg gataccactg tttgtaacaa gagcatatta tttgttcagg gggcagtcaa
    10321 tcctcattct gatagcatct gttttaatat ttctttgttt tttaagattg aatatgaaat
    10381 caataaaatg gattaacatc atagccactt caagttttgg aatttatcta attcatgatt
    10441 gtatatttat tagaatgtat ttatggcaaa aactttttag aaacgcgtca tactttagta
    10501 gtccatattt aataccgtac tctatatttg ctatttcagc tgtatttatt gggagttgtt
    10561 tatgtagttt attgatgaat aaaatgttag atggcccgat tgaaaaagtt aatagatggt
    10621 tattggcatg tacatttata aaaaacaagt aaaataaatc gaaacaagta tgaagca
//
LOCUS       DATUCG010000086        10525 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.088, whole genome shotgun sequence.
ACCESSION   DATUCG010000086 DATUCG010000000
VERSION     DATUCG010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10525
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.088"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(546..2627)
                     /gene="fusA"
                     /locus_tag="VCA20_001693"
     CDS             complement(546..2627)
                     /gene="fusA"
                     /locus_tag="VCA20_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEP1835478.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWNNHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVSTLHERLQANAH
                     PIQLPIGSEDEFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYLEQAEEYREKLVEAV
                     AETDEELMMKYLEGEEITNEELKAAIRKATINVEFFPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGVNPDTDAEEERHASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     NSGSYVLNTSKGKRERIGRILQMHANSRQEIETVYAGDIAAAVGLKDTTTGDSLTDEK
                     AKIILESIHVPEPVIQLMVEPKSKADQDKMGIALSKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMRREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPIVDIKAKLY
                     DGSYHDVDSSETAFKVAASLALKEAAKSAQPTILEPMMLVTITAPEDNLGDVMGHVTA
                     RRGRVDGMEARGNTQIVRAYVPLAEMFGYATVLRSATQGRGTFMMVFDHYEDVPKSVQ
                     DEIIKKNGGNA"
     gene            complement(3120..3590)
                     /gene="rpsG"
                     /locus_tag="VCA20_001694"
     CDS             complement(3120..3590)
                     /gene="rpsG"
                     /locus_tag="VCA20_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEP1835479.1"
                     /translation="MSRKNQAPKREVLPDPLYNSKLVTRLINRVMLDGKRGTAASIVY
                     GAFDQIKEATGNDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVTIARNRGEHTMINRLAKEIMDAANNTGAAVKKREDTHKMAEANRAFAHFRW"
     gene            complement(3607..4020)
                     /gene="rpsL"
                     /locus_tag="VCA20_001695"
     CDS             complement(3607..4020)
                     /gene="rpsL"
                     /locus_tag="VCA20_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEP1835480.1"
                     /translation="MPTINQLVRKPRKSKVEKSKSPALNVGYNSRKKVQTNVSSPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQGRSKYGTKRPKG"
     gene            complement(4214..4938)
                     /locus_tag="VCA20_001696"
                     /pseudo
     CDS             complement(4214..4938)
                     /locus_tag="VCA20_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841499.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            4997..6133
                     /locus_tag="VCA20_001697"
     CDS             4997..6133
                     /locus_tag="VCA20_001697"
                     /inference="COORDINATES: protein motif:HMM:NF018488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISLre2 family transposase"
                     /protein_id="HEP1835481.1"
                     /translation="MTIVAEICEILKSSVHLAEFDKSVMQLMSQLLTKSVRESLERLD
                     REIIQSYLSDGWEIDRLEERQLTFLFGTDSFKRRRLQKAGEKSFLPLDKVIGLNSRER
                     YSTSFNEKLSLLATGMTYRQASQSLHLLTDIEMSHQRIHNLVQRVGEKVVNSQVEPTE
                     TELKRPEFLFIEGDGVWIGSQDKGKHLELKRGYIHEGVRRFGKRGELINPVYFGCFGT
                     SQDLFKQMSDYLQMHYDLRHTVIVANSDGGSGYEAHKFEDMLGRHKSFYYCLDSYHVM
                     KYITGKLGFDKSLLQAVRMCVKAYDREQLVLILDTAESVLEDEKQLEKLLAVKSYLMS
                     HWKAIKPLKLRDLGVSDGVGTCESGHRFYSYRIKNRGETGKNKA"
     gene            6130..6351
                     /locus_tag="VCA20_001698"
     CDS             6130..6351
                     /locus_tag="VCA20_001698"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835482.1"
                     /translation="MSHLVTIMTAQKNGIFEALYRSSSPHQVFSDKVAISMRQVLKKL
                     PPEPHRVPQASIALNGATSTPIGQLKKWI"
     gene            complement(6731..7927)
                     /locus_tag="VCA20_001699"
     CDS             complement(6731..7927)
                     /locus_tag="VCA20_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEP1835483.1"
                     /translation="MYNFDSLPNRKLDKCRKWDESLLRQRFPKLPNEYIPMWIADMDY
                     ATAPEIISGLQELLLKGTLSYTYTYDEFYDVVVDWQHRRHGVKVTRDMITLVYGTVST
                     IHNIIQCFCTGSRKVIMNTPVYDPFALAAERNGVTVIANTLIVRDNRYFIDMESLEQQ
                     LQDHQPDVYLFCSPHNPSGRIWTREELVEVATICRKYGTLLVVDEVHSEHILFGNHIS
                     VGCLDVEVINQIILLTSPNKAFNIGGLKTSYALIWDEGLRCQFREQLAKNSITSPTPF
                     GITAVIAAYRRGEEWLNAVTEYIRENYLLVKERLKNDFPDWRLMDMEASYLCWVDVSR
                     GKIPASDLVPYLAEVSGVILEDGTHYVQDGENYVRINIATSRELLLTALDQIKCAYEA
                     LYPDKG"
     gene            complement(7937..9508)
                     /gene="malX"
                     /locus_tag="VCA20_001700"
     CDS             complement(7937..9508)
                     /gene="malX"
                     /locus_tag="VCA20_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HEP1835484.1"
                     /translation="MSEQSQSRRVRIFSFFQELGKTFMFPVALLAFLGILLGLGSSIT
                     SDPVIQRFPFLGHSWIQLFFQYLTMVGSLAFRYLPALFAMAIPLGLSKRNKGVGVFAG
                     LVGFIAMHMSINFYLQTTNTLAEADVMRINGQGMVLGIQSIEMGVLGGIIAGLIVYFL
                     HERFQDTVLPDAFSFFGGIRFVPIISSLVLSVVGLIIPIIWPLFSTVITSIGMLIAKA
                     GVFGSFIYGLGTNVLKPFGMHHILLAMVRFTDVGGTQVVDGETVSGALNIFYSQLKVG
                     EPISPQATAFLSQGFMPTFMFGLPAVALAIYHVAKKENRPAIKGLLMSGFLVSFVTGI
                     SEPIEFLFLFVAPVLYVFHSIMSGLSLMVMAMLGVTIGNTDGGVIDWIIFGILQGNYT
                     KWYLLIPVGAVWFVAYYFVFKWYFKKYDIATPGRKDEPMNEENGEIKVFTKGNGIHDP
                     AVILEALGGKENITNLDNCVTRLRLQVKDSSIIDKETLRKAGALGVVELDAHNIQVII
                     GAQVQSVKTGIEQLL"
     gene            complement(9539..10369)
                     /locus_tag="VCA20_001701"
     CDS             complement(9539..10369)
                     /locus_tag="VCA20_001701"
                     /inference="COORDINATES: protein
                     motif:HMM:NF013070.1,HMM:NF015107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEP1835485.1"
                     /translation="MKIVKVLNNSTVIAQDGEMELVVMGKGISFGKRPGDWITPSKIE
                     KIFWLDSGKNAVKLAKLLAEIPILYFEVVRDLIDIAEAELDISLNETIYLSLTEHIQF
                     AVQRYQEGAFTPNAMLLEIATVYQVEYQFALRCLSYLEQQLDVRLPNDEAGFIALHLI
                     LAQGNSRSRDKVTELTTTVYDLVRIVQEYYQLTFLETDPGYSRFVVHLKFFIMRLLQG
                     EQLPAEDIALYQALTTTYNRAKGVLPVITSYLERKYDYRLCDGEAFYLTLHIHRLLNR
                     "
BASE COUNT         3121 a         2223 c         2077 g         3104 t
ORIGIN      
        1 tcgcttcaaa ggttcgggga acctttgaag gctggagata gggcgaagca agttcgcctc
       61 aatagctgca ggttgctaga acagccttta ggctgttcta ggttagaaat aagacttgcc
      121 tcgcaaatca cctctgtaaa gtacagcaaa gcgagttcaa atactccagt gaagtgtttg
      181 aagttggaaa taaggaaacg aagtttcctc gctcgtcgaa gcaatcaaag ataaactact
      241 gatactcaat gaaaatcaaa agtagcctag gaaacgaagc cgaagatata gtggcttcaa
      301 ttaggaaaag gacggcttta agttatagtg ttaataaccc actcctaaaa taactttaga
      361 gttctcaaat tgacaaactt agatatgtcc cgtttctaat tcacttgact atagaactgg
      421 agttcatcaa ggcaagttga caacggataa ttttgatttt cgaagagtat gagtatcaca
      481 ctcagatgtg tgacatatcc atcgtaccaa aaaacaccta cccgaaggta ggtgtagata
      541 ggcaattatg cattgccacc gtttttctta atgatttcat cttgtacaga ttttggtaca
      601 tcttcgtagt ggtcaaatac catcatgaaa gtaccgcgac cttgtgttgc tgaacgaaga
      661 acagttgcgt aaccgaacat ttcagcaagt ggcacataag cacgaacgat ttgtgtgtta
      721 ccacgagctt ccataccatc aacgcgacca cgacgtgcag taacgtgacc cataacgtca
      781 ccaaggttat cttctggcgc agtgattgtt acaagcatca ttggctcaag gatagttggt
      841 tgagctgatt tagctgcttc tttaagggca agagatgctg ccactttgaa ggctgtttca
      901 gatgagtcga catcgtggta tgaaccatcg taaagcttag ctttaatgtc aacaattggg
      961 taaccagcaa gaacaccgtt agccatagat tctacgagac ctttttcaac cgctgggatg
     1021 aattcacgtg gaaccacacc accgacgata gcattctcga actcgaaacc tttaccttct
     1081 tcgtttggtg tgaattcgat ccaaacgtca ccgaattgac ctttaccacc agactggcgt
     1141 ttgaagaaac cacgagcttg tgtagaagcg cggaatgttt cacggtatga tacttgagga
     1201 gcacctacgt ttgcttcaac cttgaattca cgacgcatac ggtcaacaag gacatccaag
     1261 tgcaactcac ccatacctga gataactgtt tcaccagttt caacgtttgt ttcaacgcgg
     1321 aatgttggat cttcttcagc caatttagaa agggcaatac ccatcttgtc ttggtcagct
     1381 ttagacttag gctcaaccat caattggata actggttctg gaacgtggat tgactcaagg
     1441 atgatttttg ctttttcatc tgtcaatgag tcaccagttg tagtatcttt caaaccaacc
     1501 gcagcagcga tatcaccagc ataaacagtt tcaatttctt gacggctgtt tgcgtgcatt
     1561 tgaagaatac gtccgatacg ctcacgttta cctttagaag tgttcaatac gtatgaaccg
     1621 ctgttaagga cacctgaata tacacggaag aatgtcaaac gacctacgaa tgggtcagtc
     1681 atgatcttga aggcaagagc tgcaaatggc tcttcatctg atgcatgacg ctcttcttca
     1741 gcatctgtat ctgggttaac acctttaata gcagggatgt caagtgggct tggaaggtag
     1801 tcaataaccg catcaagcat caattgaaca cctttgtttt tgaaggctga accgcacaat
     1861 actgggaaga attcaacgtt gatagttgct ttacggatag cagctttcaa ttcttcgttt
     1921 gtgatttctt caccttcaag gtatttcatc atcaattctt catcagtttc agcaactgct
     1981 tcaaccaatt tttcacggta ttcttcagct tgctcaaggt attcagctgg aatatcttct
     2041 tcaagaatat ctgtaccaag gtcgttagta tagatttcag ctttcatctt gatcaagtcg
     2101 atgataccac ggaactcatc ttcagaaccg attggcaatt ggattggatg agcatttgct
     2161 tgaagacgct cgtgaagtgt gcttactgag taaaggaagt cagcaccgat tttatccatt
     2221 ttgttagcga atacgatacg tggaacaccg tattcagttg cttgacgcca tactgtttca
     2281 gtttgtggct caacacctga ttgtgagtca agaacggtta ccgcaccatc aagaacgcga
     2341 agtgaacgtt gtacttcgat tgtgaagtcc acgtgtcctg gtgtgtcgat gatgtttaca
     2401 cggtggttgt tccattgagc tgttgtcgca gcagatgtga tagtgatacc acgttcttgc
     2461 tcttgctcca tccagtccat ttgcgatgca ccttcgtgag tttcaccgat tttgtggatt
     2521 ttaccagtgt agtaaagaat acgctcagtt gtcgttgttt tacccgcgtc aacgtgcgcc
     2581 atgataccga tattacgagt tttttctaat gaaaattcgc gtgccatgag gtttgttctc
     2641 ctatattttt agatttctat tctgattata ccatatttta agaaaagcga gtgggcaaat
     2701 cccacccgct taagttctta ctaatggtgt gaagtggacg aggcaagcct caatccgatt
     2761 tgtgatggtt gaacgataag ttgctaccat aatctcaatt gatttatgta gaactgactt
     2821 aacgtcctca catcctaatt gaactcgggc tacaactcgc tgacaaaaag agacgcagtt
     2881 gttttggctt tagtcaatta caaatggggc tacctttagg ggagaacctc attgattgca
     2941 cagcaatctg cttcgcttcc ctattttgtc taggagttgc ttgacgccct cgtatcttaa
     3001 tcttagttga attttagcta caaatgtctg ccaaaaagat aaaatctcct agaaactgaa
     3061 gtttctgcgt caatttccct attttgtcta gcatttgttt aacgctaaaa tatcctatct
     3121 taccagcgga agtgtgcgaa tgcgcggttt gcttctgcca ttttgtgagt atcttcacgt
     3181 ttcttaacag ctgcacctgt gttgtttgct gcatccatga tttctttcgc aaggcgattg
     3241 atcatagtgt gctcaccacg gttacgagcg attgttacca accaacgaag accaagtgtt
     3301 gtacgacgct ctggacgaac ttcaactggg acttggtagt tagaaccacc gacacgacgt
     3361 gcacgtactt caagtacagg catgatgttt tccattgctg tttcaaatac ttcaagtgca
     3421 tcgttaccag ttgcttcttt gatttgatca aaggcaccgt aaacgattga tgctgcagta
     3481 ccacgtttac cgtccaacat aacacggtta atcaaacgag ttaccaattt tgagttatac
     3541 aatggatctg gcaatacttc gcgcttaggc gcttgatttt tacgactcat ttatatttcc
     3601 cctttcttag ccttttggac gtttagtacc gtatttagaa cggccttgct tacgatcgtt
     3661 aacacctgca gtatcaagcg caccacgaac gatatggtaa cgtacccctg gaaggtcctt
     3721 tacacgtcca ccacgaagaa gaacaacgct gtgctcttgc aagttgtgac cgatacctgg
     3781 gatgtaagca gtaacctcga taaggttgct caaacgtaca cgagcaaatt tacgaagggc
     3841 tgagttaggt tttttaggtg tcattgttcc gacacgagtt gcaacaccgc gtttttgtgg
     3901 tgatgaaacg tttgtttgaa cttttttacg gctgttgtaa ccaacgttca aagctggtga
     3961 tttagatttt tctactttag acttacgtgg tttacgtacc aactggttaa ttgtaggcat
     4021 ctacattctc ctgtatatat tttttatttt tggtgataag gcattggtga caacccctat
     4081 ctgtgtgtac ttttgcaaca tttgtcagca cgtccctgta cactcttgag agaccaaaag
     4141 taaaaagtac cgtctaatat aataccacag ccaactgtct ttgtcaaacg gttatgattg
     4201 gatttttaca aaatcaacaa tcaataagtc gacccaaaag aaattcactc ggtgacttgt
     4261 aatccaagca agggtagttg ttcacccact tttagataaa agcgacttct ttgggagtcg
     4321 ttttttggtt cctttagata accatctccg aactaaaaga ttatgatttt catttgtccc
     4381 tctctcccat aaagagtagg gatgagcgca atagatattt tcattggaga atccttctgc
     4441 caatcgtttg aactccgaac cattattcgc agcaatagac agaatgcgat actcccctga
     4501 aattaacctg agcgcctgat tgacgattcg gcagttttat ttagttaatc ctaagtgtcc
     4561 attgtgaaag caacagtaaa tagttgaaat acccactgga atttatttct ttttaaccat
     4621 catttcgggt gaaaattttt gtttatgata gtgtaaagtc ttctctttta tttctttgct
     4681 taagattaat tttttaccga cctctttcga ttgttttggt aaacagtcgg tgcctaatcg
     4741 gctgagtaga cctttttata cccctttttg cacttgttgt aaggctgtac cccgattgac
     4801 ctcgttatta atggtctgag aagcctttcc aagtaagtcg gcaatttcac gattagactt
     4861 gccctcattc ttccaccgta caatcaggcg acgggttagt gtaaaataat tatcagaaaa
     4921 acgtttacaa gttttcaaaa taaaaaagtc ctatatccta ggaagtgcgt aaataaccta
     4981 gaaggatata gaacccatga ctattgtagc agagatttgt gaaattttaa agagtagtgt
     5041 tcatttggct gagtttgaca agagtgtcat gcagttgatg agtcagttat tgacaaaatc
     5101 agtgagagaa tccttggaac gtttggacag ggaaattatc cagtcttatc tctcggatgg
     5161 atgggagata gaccgtttag aagagcgtca actgactttc ttgtttggga cggattcttt
     5221 taagcgtcgg cggttacaaa aagctgggga gaagagtttt ttgcctttgg ataaggttat
     5281 agggttgaat tctagagaac gttatagtac tagtttcaat gaaaaactta gccttttagc
     5341 aacaggaatg acctatcgtc aggcctccca aagtttacat cttttgacag atatcgagat
     5401 gagtcaccaa aggattcata accttgtcca gcgtgttggt gagaaggtgg taaatagcca
     5461 agtagagcct acagaaactg agttaaaacg cccagaattt ctctttattg agggtgatgg
     5521 tgtttggatt ggtagtcaag ataaagggaa acacttggag ttgaaacgtg gctacattca
     5581 tgaaggtgtg agacgttttg gcaagcgtgg cgagttgatt aaccctgtct atttcggttg
     5641 ttttggtaca agtcaggact tgtttaagca aatgagtgac tacctccaaa tgcactatga
     5701 cctgaggcat acggttattg ttgccaatag tgacggcggt tctggctacg aagctcacaa
     5761 gttcgaagac atgcttggac gacacaagag cttttactac tgccttgatt cctaccacgt
     5821 tatgaaatat attactggaa aattaggctt tgataaaagc ttgttacaag cggtgcggat
     5881 gtgtgtaaaa gcctatgacc gtgagcaact ggtcttgatt ttagacacgg cagaatccgt
     5941 tttggaagat gagaaacaac ttgaaaagct attggcagtc aagtcttatc tgatgagcca
     6001 ttggaaagcc atcaagcccc taaaactacg ggatttgggc gttagtgatg gtgtaggaac
     6061 ctgtgaaagt gggcatcgat tttacagcta ccgcatcaag aacaggggcg aaactggaaa
     6121 aaacaaggcg tgagtcatct agttaccatc atgacggcac agaagaatgg aatttttgag
     6181 gctttatatc gtagttctag cccacatcaa gtctttagtg acaaggttgc aatcagtatg
     6241 cgccaagtac taaaaaaact accgcccgag cctcataggg tgcctcaagc aagcattgct
     6301 ttgaacggtg ctaccagcac tccgattggt caactcaaga aatggatata ggactttaaa
     6361 aactttccga gagaaacttg acacaaacag gcgacggtta tcaattgtca aatattttcc
     6421 ttttggtgtt taatattttt gcatctctgt acctctttct tgtgtttgtg acgaactaca
     6481 agtatggcac agaggtgttt tcttatacct taaaatggat ctcctttgac aacaggaacc
     6541 cttgaagctg ttatcaaatg aaatcaattg cttactccaa gagggtaaaa catctgattt
     6601 tgattaaggt aaggtggcta attttattat agaattttca atcaataagt cgaccattct
     6661 tacttgggat ctttgaataa gttaaggaag acggcctaaa atagatcgtc ttcttatttt
     6721 ggtttttcag ctacccctta tcaggataaa gagcctcgta ggcacactta atctggtcca
     6781 gagcagttag caagagctct cttgatgttg ctatattgat ccgcacgtag ttctctccat
     6841 cctgcacata gtgagtccca tcctctagga tgacacctga tacttctgct aaatagggga
     6901 ctagatcact tgccggaatt ttccctctcg acacatcaac ccagcaaaga taagaggctt
     6961 ccatatccat cagtcgccaa tccggaaaat cattttttaa tcgttctttc acaagtaagt
     7021 aattttctcg tatgtattcc gtaacggcat taagccattc ttctcctcgt ctataggctg
     7081 caataacagc agttatccca aacggcgtcg gagaggtgat gctattctta gctagctgct
     7141 ctctaaattg acaacgtaac ccttcatccc atataagagc ataagaagtc ttcagaccac
     7201 caatattaaa agctttgtta ggtgatgtta ataaaataat ctgatttatc acctccacat
     7261 ctaagcaacc aacggaaata tggtttccaa ataaaatatg ctccgaatgg acttcatcca
     7321 caactagtaa tgttccatat ttcctacaga tagtcgccac ttccaccagc tcctctctag
     7381 tccagatacg acccgaggga ttgtgaggag agcaaaacaa atatacatcc ggttggtggt
     7441 cttgtagttg ttgttctaaa ctttccatgt caataaaata gcgattatct cgaacaatca
     7501 gagtattagc tattactgtg actccatttc tttcagctgc tagagcaaat ggatcataga
     7561 ctggagtatt cataataact tttcgagagc cagtacaaaa acactgtata atattatgga
     7621 ttgtcgaaac agtcccataa acgagcgtta tcatgtccct agttacttta acgccatgtc
     7681 tgcgatgctg ccaatcaact acaacatcat agaattcgtc ataagtatat gtgtagctca
     7741 aggttccttt gagaagcaat tcttgtaagc cgctaataat ttcaggtgcc gtagcataat
     7801 ccatgtcagc aatccacata ggaatatatt catttggtaa cttgggaaaa cgttgccgta
     7861 aaagagactc atcccacttt ctacacttat ctagtttacg gttaggaaga ctgtcaaaat
     7921 tatacataat atcctcctat agcaactgtt ctatcccagt cttaacactc tgaacctgag
     7981 cccctataat cacttgaata ttatgagcat ctagctcaac tactcccaaa gcacctgcct
     8041 tacgaagcgt ttccttatca atgatactag aatctttgac ttgcaaacgt aaacgtgtaa
     8101 cgcaattgtc tagatttgtg atattttctt ttcctcccaa ggcttccaag atgaccgctg
     8161 gatcatggat gccattacct ttggtaaata ctttaatctc cccattctct tcattcatag
     8221 gctcatcttt acgtccaggc gtagcaatat catatttctt aaagtaccat ttaaagacaa
     8281 aataataagc tacaaaccat accgctccaa cggggataag tagataccat ttagtataat
     8341 ttccttgaag aatgccaaaa ataatccaat caatgacccc tccatcagta tttccgattg
     8401 tcacaccaag cattgccata accataaggg acaagcccga catgatggag tggaaaacat
     8461 acaaaactgg ggcaacaaat aagaatagaa actctatcgg ctctgaaata cctgttacaa
     8521 atgaaactag gaagcccgac atcaataaac cttttatagc aggtctattt tcttttttag
     8581 caacatgata aatggctaga gcgacagctg gaagaccaaa cataaacgtt ggcatgaagc
     8641 cttgagataa gaaagctgtt gcttgaggac taatcggctc cccaacctta agttgcgaat
     8701 aaaagatatt gagagctcca gaaacggtct ccccatcgac aacttgagtc cctcctacat
     8761 ctgtaaaacg aaccatagct aatagaatat gatgcatacc gaagggtttc aaaacattag
     8821 tacccagtcc gtaaataaaa gaaccaaata ctccagcttt agcaatcaac atgccgatgg
     8881 atgtgatgac tgttgaaaac aggggccaga taatcggaat aatcaaaccc acaacagaca
     8941 ataccagaga tgaaataatc ggtacaaatc ggatacctcc aaagaaagaa aaagcatctg
     9001 gcaataccgt atcttgaaaa cgctcatgta aaaaatagac aattaagccg gctatgatac
     9061 ctccaagcac ccccatctca atagattgaa taccaagcac catcccttgc ccgtttattc
     9121 gcattacatc tgcttctgcc aaggtattgg ttgtttgaag atagaaattt atagacatgt
     9181 gcatggctat aaaacctacc aaccctgcaa aaactcctac acctttattg cgcttagata
     9241 aacccagtgg tattgccatg gcaaaaagag caggtaaata tcgaaaagct aagctcccaa
     9301 ccatcgtaag gtattgaaag aagagttgaa tccatgaatg acctaaaaac ggaaatcgtt
     9361 gaataactgg atcggaggta atcgaactac ctagtcccaa tagtattccc aagaatgcga
     9421 gtaaggctac cgggaacatg aaggtcttcc ctagctcttg gaaaaagcta aatatgcgca
     9481 cacgacgtga ttgactttgt tctgacataa agtaaacctc ctttcaaaat gaaaataatt
     9541 aacgatttaa aagccgatgg atatgaagtg tcaaataaaa ggcttcccca tcacataaac
     9601 gatagtcata ctttctttct aagtaactag ttattacagg aagaactcct ttcgctctat
     9661 tataggttgt tgtcaaggcc tgataaaggg cgatatcttc ggccggaagt tgttctcctt
     9721 gtaacagacg cataataaaa aatttcaaat ggacaacaaa acgactatat cctgggtctg
     9781 tttctaaaaa agtcaattga tagtactctt ggactatgcg caccaaatcg tagactgttg
     9841 ttgtcagctc tgttaccttg tctcgcgatc gagaatttcc ttgggcaaga atcaaatgga
     9901 gggcaatgaa cccagcttca tcatttggca aacgcacatc caactgctgt tctaagtaag
     9961 ataagcaacg aagagcaaat tggtactcta cttgatagac agtggctatt tccagcaaca
    10021 tcgcattcgg agtgaaagcc ccctcctgat accgctgaac ggcaaactgg atgtgctccg
    10081 ttaaacttag ataaattgtc tcattcagtg atatatccag ttctgcctct gctatatcaa
    10141 ttaagtcacg taccacttca aagtataaaa ttggtatctc tgctaaaagc ttagctagtt
    10201 taacagcatt tttcccgcta tctagccaaa atattttttc aatcttacta ggagtaatcc
    10261 aatcaccagg acgtttccca aaagaaatcc ctttgcccat cacaactagc tccatctccc
    10321 catcttgggc aataacagtg ctattattta gtactttgac aattttcatc gccaaactcc
    10381 ttcctaaaac agacaaaaag acccacatca aaaaaactgt ctaaacaatt aatttgatat
    10441 aggtcttgct ctatcgataa caccctattt ctgtttttat tataacaaaa tatcatatct
    10501 tcgtcaacct attttcttat gattt
//
LOCUS       DATUCG010000087        10419 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.089, whole genome shotgun sequence.
ACCESSION   DATUCG010000087 DATUCG010000000
VERSION     DATUCG010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10419
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.089"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            71..1195
                     /locus_tag="VCA20_001702"
     CDS             71..1195
                     /locus_tag="VCA20_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HEP1835486.1"
                     /translation="MKLPRFGVEEWLNVHENSAIYDIAGVSISSLTLEELLALSGTNP
                     EDFYEKLQGTKLNYGWIEGSPAFKEAVSQLYEHVGPDQILQTNGATGANLLVLYGLIE
                     PGDHVISLYPTYQQLYDIPKSLGAEVDLWQIEEENGWLPDLEKLRQLIRPNTKMICIN
                     NANNPTGAVMDRAYLEELAEIAGEVGAYILSDEVYRSFSGLDVPSIIEVYDKGIAVNS
                     LSKTYSLPGIRVGWVAANHQVTDILRDYRDYTMICAGVFDDLVAQLALAHYQEILERN
                     RRILEENLAILDQWIEEEPLVSYIRPAVVSTSFVKIAVDMPMEDFCLQLLQEHGVLLV
                     PGNRFERDGYVRLGFACEQETLIKGLEKLSQFLRRFDKEN"
     gene            complement(1210..2379)
                     /locus_tag="VCA20_001703"
     CDS             complement(1210..2379)
                     /locus_tag="VCA20_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEP1835487.1"
                     /translation="MPNLLTYLEETQYDNFYDKPINKLDILALTELSYLPFDNLVPYS
                     FTENGVRLDRLAAAFEETYKNNFPPFSMVTKNRLALLGLLAKSKRFKSIRAFGFVDDY
                     QLEQEKQFSAISYRIDRKTIVTCFRGTDDTIIGWKEDFHMTYMDEIPAQRAASGYLEK
                     LMMQQGGHFYLAGHSKGGNLALYAACQQATDQQDRILAIYPFDSPGLHKKHLEAPSYQ
                     AIHQRIFPVIPQNSIVGMMLETPENAQIVQSNTLGILQHISFSWEVDGSDFKLAPALT
                     SDSLQTDQALKTWTASLTDDELRDFFDLFFGIFIKAGIERFSDITVNPLQKLQEIDRL
                     RKEIAPEEAEMVDKLIRLLFDTRYQIWRDNIPSPEISLPDWRKLFQRNTTENKEN"
     gene            2549..3232
                     /locus_tag="VCA20_001704"
     CDS             2549..3232
                     /locus_tag="VCA20_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEP1835488.1"
                     /translation="MQKILLIEDDKTISQLVAKNLINWGYQVQEVRDFQMVLEQMEEF
                     QPHLILLDIGLPFFNGYYWCQEIRKTSRVPIMFLSSHDQPMDIVMAINMGADDYVTKP
                     FEMPVLLAKIQGLLRRTYDFVGEQSVLWFEEICLDLKTMQVSYEEAVEELTRNEFQIL
                     RVLFEHGKEVVSREELMRELWNSDIFVDDNTLSVNIARLRKKLAELGLPDLIMTKKGV
                     GYGLVWTHE"
     gene            3225..4205
                     /locus_tag="VCA20_001705"
     CDS             3225..4205
                     /locus_tag="VCA20_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEP1835489.1"
                     /translation="MNKKMLTDFLTSWLRHRWLFLVSYCLFVTTILAYSSLFDIQRNL
                     VFYALTLLTICWLLALLWDFVREFSRFGNIWRGQKVTTGTASERLLQEKVERLEVQNK
                     LLIEKQRQEQTELDDYYTLWAHQMKTPIAASQLLVKEVDSSPVRHQLETELFKIEQYT
                     GLVLNYLRLQSFHDDLVIEAVNLEELVRSLVKKYSLFFIQANTSLDLGQLDRTVKTDK
                     RWLGLLIEQILSNALKYCQEGTISIVLDGDDLVIRDTGIGIAESDLERVFERGFSGFN
                     GRRTQQSSGLGLYLSRKIAGELGYSLKLKSKVGRGTEVRIGIKEVELIFD"
     gene            4301..5059
                     /locus_tag="VCA20_001706"
     CDS             4301..5059
                     /locus_tag="VCA20_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1835490.1"
                     /translation="MSVLDVQHVQKIYSSLFKAGQVEALKDIHFTVEKGEYVAIMGES
                     GSGKSTLLNILATLDKPTQGQVLLNGLDTQSIKSKEAAVFRREKLGFVFQDFNLLDTL
                     SVKDNVLLPLVLSRYPIDEMNKRLVHTLNSLGIATLQDKMPYEISGGQQQRVAVARAI
                     ITRPEILLADEPTGALDSKSTATLLDIFEKINQEGQTILMVTHSTVAASRAKRVLFIK
                     DGILYNQIYRGERTSQEMYQLISDTLTLMANRGE"
     gene            5061..7061
                     /locus_tag="VCA20_001707"
     CDS             5061..7061
                     /locus_tag="VCA20_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEP1835491.1"
                     /translation="MFGLTVRLALTNLKKNRRLYFPYALMTIVSTAIAYIFASLAFHP
                     DLGQVKGADGVTMVLGFGMIVVMLAVAIMVLYANSFVMKQRSKEFGVYSILGLEKKHL
                     LLMTFLENLVFSASTILSGLLLGLALDKLFYALLLKAMQMPVVLASTFQLKSLVTVLI
                     YLFGIFALVSLFNIGKLGLTDSLKLVQGKKRGDKKSGFLWLQTLLALILLGAGYAIAQ
                     LVTSPLTAIPSFFGATLLVILGTYLLFHAGVISLLNWLKNRQTYYYKPENFISVSNLI
                     FRMRKNALGLATITILSSMFLVTMVGGLNIYIGGKDYIANQNPNDFSIDVGMQPTTSK
                     GQTEEWMAAILEEKDIPVESKVVYPYQQAYFTEVTNQQVRFFTVEDGASIAFSVLGIG
                     IIIDQANYEKMTGEALQLASDQVAIYSKGVQYQAGQTLTFDEKEMEVVQVLPKNVTLG
                     HLPDYMSFNVNQYLVVVVQDLTIFENQAENRYYMGFESSLSEEEQVNSQEIFLSGTFE
                     SELWSSNILPDGTNSISLAYRAYATRNFFSFAGSLFFIGLLLSLIFLMAVVLVIYFKQ
                     ISEGYEDRSRFVIMTKVGLDEDQTRQSIRKQLLTVFFLPILLAFVHLAFAYKMLSSIL
                     LSIGVVNAVLMLQVTAGICGGYLLLYLVVYAITTRSYKQIIS"
     gene            7248..7412
                     /locus_tag="VCA20_001708"
     CDS             7248..7412
                     /locus_tag="VCA20_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835492.1"
                     /translation="MKLDKKSRENWKLAGITLVTAIITGWALDSILILGILLVSGLLF
                     GFFTDDEEGL"
     gene            7409..7726
                     /locus_tag="VCA20_001709"
     CDS             7409..7726
                     /locus_tag="VCA20_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEP1835493.1"
                     /translation="MKETQMLKGVLDGCVLQVIARQEIYGYELVQELRKLGFENMVGG
                     TVYPLLQKLEKNSLIFSQNKPSPDGPDRKYFYLTDQGKAYLEDFWSQWTELVQKVQRL
                     KGE"
     gene            7728..8522
                     /locus_tag="VCA20_001710"
     CDS             7728..8522
                     /locus_tag="VCA20_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835494.1"
                     /translation="MSKQMEYRKQIEVIESQLTKENKEYMGRINGYMMIASVFHRQEE
                     AVTAQLLSIYQDVLEAQKDGLSAEDFLGKDSKQMADDLLSYLPPIGFMEVANLSGLML
                     IIYLGSQWLMDFAGTGTISINWLGLICDAVLSLLLPSGIFLIIRGLIYQTSKIKVWAS
                     FLCIPLLFLLICGLRLWAIPKEPDLVLTGWGLLVPLTLLGLALLFFQKEKLVRYVFMP
                     SYLLMMVGGVVNMVMTVPVWLNLMLVILPAMAFWIGSAVLLVRKEK"
     gene            8599..9348
                     /locus_tag="VCA20_001711"
     CDS             8599..9348
                     /locus_tag="VCA20_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835495.1"
                     /translation="MRKLHAYLMLASAFHEQEERATELLLSIYQDVLDAQADGQSAED
                     FLGKDSKQMADELLSDLPPIRWYYGLRLTGLISLVYISWFFLDLFGVTGEMFLEWRGL
                     LCDLLLALILPSSAFFILKNLVYERSKIKSYLLMGMWGLSFVGLIVLRFWISRQFYEG
                     VPLPLWASILILIGIAVGIWYYRKKSPIVYVFVPAYLLTVLRGFSQVTASQFGYGPQD
                     WTGWLPLLFMVLAFFSTLAGTFFLLKKENMT"
     gene            9425..10165
                     /locus_tag="VCA20_001712"
     CDS             9425..10165
                     /locus_tag="VCA20_001712"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEP1835496.1"
                     /translation="MNEQQQRKELHQKIWAIADDVRGAVDGWDFKQYILGILFYRFIS
                     ENIADYFDQAEHAAGDKEFSYALLTDEEAEEDFRQGTVEEKGFFILPSQLFVNVVAKA
                     RTNENLNTDLATIFEQIEKSAMGHESENDIKGLFDDVDTRSNRLGGTVAEKNKRLADI
                     LLGISEIDFANFQDNEIDAFGDAYEYLISNYASNAGKSGGEFFTPQTVSKLLAHLVMV
                     GKDKINKVYEKKIKDWIHKPTLYSLKSA"
BASE COUNT         2832 a         2008 c         2461 g         3118 t
ORIGIN      
        1 tcgcatattt atacaattgt tgaaagaata tggtataata gggaaagatt aaattgaagt
       61 aggaagtctt atgaaattac cacgttttgg cgttgaggaa tggctaaatg tgcatgaaaa
      121 cagcgcgatt tatgatatag caggggtgtc tatttcttca ttgactttgg aggaactact
      181 tgccttgtcg ggcaccaatc ctgaggattt ttatgagaaa ttgcagggca ccaagctcaa
      241 ctatggctgg atagaaggtt cgccagcatt taaagaagct gtcagtcagc tctacgaaca
      301 tgtaggacca gaccaaatcc ttcaaaccaa tggagcgacc ggtgctaact tgttggttct
      361 ttatggtttg attgaaccag gagaccatgt catttccctt tacccaacct accaacaact
      421 ctacgatatt ccaaagtctt tgggagcaga agtggatttg tggcagattg aggaggaaaa
      481 tggctggctg ccagacttgg aaaaactgcg tcagctcatt cgtcctaaca ctaagatgat
      541 ttgcatcaac aatgccaata atccaaccgg cgctgtcatg gatagggctt atttggaaga
      601 attggcggaa attgctggtg aagttggtgc ttacatccta tcagatgaag tctatcgttc
      661 cttttctgga ctggatgtgc catctattat agaagtctat gataaaggaa ttgctgtaaa
      721 tagcttatcc aagacctatt ctctgccagg tattcgtgtc ggctgggtgg ctgccaatca
      781 tcaagtgacg gacattctga gagattatcg ggattacacc atgatttgtg caggtgtctt
      841 tgacgatctg gtggctcaat tagctttggc ccattatcaa gaaattctag aacgaaatcg
      901 gcgcatttta gaagaaaatt tagctatttt ggaccaatgg attgaggaag aacccctcgt
      961 ttcctatatc cgtccagcgg ttgtttctac cagctttgta aaaattgcag tggatatgcc
     1021 tatggaagat ttttgcttgc aacttctgca agaacatggt gttcttctag tgcctggcaa
     1081 tcggtttgaa cgtgacggct atgtccgtct aggttttgcc tgtgaacaag aaactctaat
     1141 caaaggatta gaaaaattat ctcaattttt aagaagattt gataaagaaa actagctcag
     1201 gtggttgagc tagttttctt tattttcagt tgtatttctt tggaaaagtt tccgccaatc
     1261 tggtagagag atttcagggc ttggaatatt gtccctccag atttgataac gggtatcaaa
     1321 gaggagccga atgagtttgt ccaccatttc ggcttcttct ggagctattt cttttctcag
     1381 tcgatcaatt tcctgtaatt tctgtagggg atttaccgtg atgtcgctaa aacgttcaat
     1441 tcctgctttg ataaatatgc cgaaaaacaa atcgaagaag tctcgtaatt catcgtctgt
     1501 tagactagcc gtccaagttt ttaaggcttg gtctgtttgt aggctatcgc tggtcagggc
     1561 aggggctagt ttgaagtcgc ttccgtcaac ctcccaagaa aaactgatat gttgtagaat
     1621 gccgagggta ttactctgga caatctgagc gttttctggt gtttctaaca tcataccgac
     1681 gattgaattc tggggaatga cagggaaaat ccgttggtga atagcttggt aactaggtgc
     1741 ttccaagtgt tttttgtgta agccaggtga atcaaaggga tagatagcta ggattctatc
     1801 ctgttggtct gtagcttgct ggcatgctgc atagagggct aaatttccgc ctttcgaatg
     1861 accggccaga taaaaatggc ccccctgctg catcataagt ttttctaagt agccactcgc
     1921 tgcacgttgg gcaggaattt catccatata ggtcatgtgg aaatcttcct tccaaccgat
     1981 aatagtatca tctgtccctc tgaagcaagt tacaatcgtt tttcggtcaa tgcgatagct
     2041 gattgctgaa aactgctttt cctgttccaa ctgataatcg tcaacgaagc caaatgccct
     2101 gatagatttg aagcgtttgg atttggctag aagtccaagt aaggctaggc ggtttttggt
     2161 caccatagaa aagggaggga aattattttt gtaggtctct tcaaaggccg ctgccaaccg
     2221 atcaagtcgt acaccatttt ctgtaaaaga gtaggggacc agattatcaa aagggaggta
     2281 ggaaagttcg gtcagagcca gaatatctag cttattgata ggtttatcat aaaaattatc
     2341 gtattgagtt tcttcaagat aagttaataa atttggcata gcagttcttt ccttttttat
     2401 ctagtataac gaaatatgat tggtctgaca aatctgcacc aatgatgacc tggttttata
     2461 gtctacttct tgtgtaaatg tataaaaata ctcaaatttg ttccaatgta agctataata
     2521 aatagtagtg atatgaaagg agatagttat gcagaaaatt cttcttatcg aggatgataa
     2581 aacgattagc caactagttg ctaaaaactt gataaactgg ggttaccagg tgcaagaggt
     2641 cagggatttt cagatggttt tggaacagat ggaagagttt cagccccatt tgattttgct
     2701 ggatattggt ctgccgtttt tcaatggcta ttactggtgt caggaaattc gcaagacatc
     2761 ccgtgtgcct attatgtttc tgtcttccca tgaccagcca atggatattg ttatggcgat
     2821 caatatgggg gcggatgact atgtgaccaa gccctttgaa atgccggttc tcctggccaa
     2881 aatacagggt cttctcagaa gaacctatga ctttgtcgga gaacaaagtg tgctctggtt
     2941 tgaagagatt tgcctagacc taaagaccat gcaggtgtcc tatgaagagg ctgtagagga
     3001 attgacccga aatgaatttc agattttacg tgttttattt gaacatggca aggaagtcgt
     3061 cagccgcgag gagttgatga gagaactttg gaatagtgat atttttgtgg atgacaatac
     3121 tttgtcggtt aacattgctc gcctgcgtaa aaaattggca gagctgggtt tgcctgactt
     3181 aatcatgacc aagaaaggag taggctacgg cctggtgtgg acacatgaat aagaaaatgt
     3241 tgactgattt tctaacttct tggctccgtc atcgctggct atttctggtc agctactgtc
     3301 tttttgtcac gaccattctg gcttatagta gcctctttga catccaaaga aacttggttt
     3361 tctacgccct caccctgctc actatctgct ggcttctcgc cctcctctgg gattttgtca
     3421 gggaattttc ccgctttgga aacatctggc gtggacagaa agtgactact gggacagcca
     3481 gtgagcgttt gttgcaggaa aaagtggagc gactagaagt ccagaacaag ctcttgattg
     3541 aaaaacagcg tcaggaacag acagaattag atgactacta caccctttgg gcccaccaga
     3601 tgaaaacgcc gattgcagcc agtcagcttt tggtcaagga agtagatagt agtccagtcc
     3661 gtcatcagct agaaacagaa ctcttcaaga ttgagcaata cacaggcttg gtcttgaact
     3721 atctccgttt acagtctttt catgatgatt tggttatcga agccgtcaat ttggaagagc
     3781 tggtcagaag tctagtcaag aaatattccc tatttttcat tcaggccaat accagcttgg
     3841 acttggggca gttggatagg acagtaaaga ccgacaaacg ctggctgggc ctgttgatag
     3901 aacaaatttt atccaatgcc ctcaagtatt gtcaggaagg aaccatttcg attgttttgg
     3961 atggggatga tttggttatt cgagatacgg gaattgggat tgcagagagt gacttggaac
     4021 gggtctttga gcgtggtttc tcaggtttca atggtcgcag aacccagcaa tcttcgggcc
     4081 tggggctcta tctctcacgg aaaattgctg gggagctggg ctattcccta aagctaaaat
     4141 caaaagtggg tcggggaaca gaggtgcgaa tcggtatcaa agaagtggag ttgatttttg
     4201 attaatctta caaaactgtc acgtaaatga aagctaaggc tatggtagtt tatcttggga
     4261 ccgtgtagaa tgaaggtaga aaaagaaaag gagtcaagtt atgtcagttt tagatgttca
     4321 acacgttcaa aagatataca gttctctctt taaggcggga caggttgaag ctttgaagga
     4381 tattcatttt acagtggaaa agggtgaata tgtcgccatt atgggtgagt ctggttctgg
     4441 aaaatccacc ttgctcaata ttttggcaac ccttgataag ccaacgcagg gccaggtctt
     4501 gcttaacgga ttggataccc aatctatcaa gagcaaggaa gcggcagtct ttcgtcggga
     4561 gaaattgggc tttgtcttcc aagatttcaa tctcttggac accctctctg tcaaggacaa
     4621 tgttctctta cctctggttt tatcccgcta tccgattgat gagatgaaca agcgactcgt
     4681 tcatacgctc aatagcctgg gaattgcgac cttgcaagac aagatgccct acgagatttc
     4741 gggtggtcag cagcagcggg ttgcggtggc gcgtgcgatt atcacacgac ctgaaattct
     4801 tttggcagat gagccgactg gagctcttga ctctaaatca acggcaactc ttctcgatat
     4861 ttttgaaaaa atcaaccaag aggggcaaac cattttgatg gtaacccact cgacagtagc
     4921 agctagtcgt gccaaacggg ttctcttcat caaggatggc atcctctaca atcaaatcta
     4981 ccgaggtgag agaaccagtc aggaaatgta tcagttgatt tcagatacct tgacacttat
     5041 ggcgaaccga ggtgagtagt atgtttggac taactgttcg attggctctg accaatctca
     5101 aaaagaaccg cagactctat tttccctatg ccctaatgac tatagtttca acagccattg
     5161 cctatatttt tgcatcgctt gcctttcatc ctgatttagg tcaggtcaag ggagccgatg
     5221 gggtaaccat ggtattgggc tttggtatga ttgtggttat gctggcagtt gccatcatgg
     5281 ttctctatgc caatagtttt gtcatgaagc aacggtcaaa agagtttggt gtctatagta
     5341 tcctaggctt ggagaaaaaa caccttctct tgatgacgtt tttggaaaat ctggtttttt
     5401 cagccagcac tattctgtct ggcttgctct tgggactggc tctagataaa ctcttctatg
     5461 cgctcttgct caaggccatg cagatgcctg tggtcctggc ttcaaccttc cagttgaaaa
     5521 gtctggttac tgtacttatc tatctctttg ggatttttgc tttggtcagc ctctttaata
     5581 ttggaaaact gggcttgact gactctctca aactggttca aggtaagaag cgtggggata
     5641 agaaatcagg cttcctctgg ctacaaaccc tcttagccct tatcctattg ggggcaggct
     5701 acgccattgc ccaactagtg accagtccgc tgacagctat tccaagtttc ttcggagcga
     5761 ctcttctggt tatccttggg acctacctgc tcttccacgc aggtgttatc agtctcttaa
     5821 actggctaaa gaaccgtcag acctattatt acaagccaga gaattttatt tctgtgtcta
     5881 acttgatttt ccgcatgcgg aaaaatgctc tgggccttgc gaccattacc atcctctcca
     5941 gtatgttctt ggtgaccatg gttggaggac tcaatattta catcggtggc aaagattata
     6001 ttgccaatca aaatccaaat gacttttcga ttgatgttgg aatgcaacca accacttcaa
     6061 aaggtcaaac ggaggagtgg atggcagcta tcttagaaga gaaggatatt cctgttgaaa
     6121 gtaaggtggt ctatccttac cagcaagcct attttactga ggtgacaaat cagcaagtaa
     6181 gattttttac ggttgaagat ggagctagta tagctttttc tgtcctgggt atcggtataa
     6241 tcattgatca agccaactat gaaaagatga ctggagaagc actccagttg gcaagtgatc
     6301 aagtagcaat ttatagtaag ggagtccagt atcaagctgg tcaaaccctc acctttgatg
     6361 aaaaggagat ggaagttgtc caagtcttgc caaagaatgt gaccttgggg catctgcctg
     6421 actatatgtc tttcaatgtc aatcagtact tggtcgttgt ggttcaagat ttgacgatat
     6481 ttgaaaatca agctgagaat cgctattata tgggctttga aagcagtcta tcagaagaag
     6541 aacaggtaaa tagccaggag atcttcttat ccggtacttt tgaaagcgaa ctgtggagct
     6601 ctaatattct tccagatggg accaactcga ttagtctggc ctaccgtgcc tacgcaacac
     6661 gtaacttctt tagttttgca ggttcacttt tcttcatcgg cctcttgctt tcacttatct
     6721 ttttgatggc ggtggtgctg gtgatttact tcaaacagat ttcagaaggc tatgaagacc
     6781 gtagtcgctt tgtcatcatg accaaggttg gtctggatga ggatcagacg agacagtcca
     6841 ttcgcaagca attgctgacc gtctttttcc tgcctatcct acttgccttt gtccatcttg
     6901 cctttgccta caagatgcta tcatccattc ttttgtctat cggcgtggtc aatgcagtgc
     6961 ttatgttgca ggtgacagca ggcatctgtg gtggctatct cctcctctac ctagtggtct
     7021 atgccatcac aacacggtct tacaaacaga ttatttccta aacttggggc aacccaagtt
     7081 tttaattttt tgaatagttt tcactatttt gttttgcata ctagccttct tgtgttatac
     7141 tatagataca aagctactag gtaaaacaaa ctagtgatag aagacaaaga aaatcacttt
     7201 tatttttatc aaactagtcg gtattacaaa ctaggtaggg aggcagtatg aaactggata
     7261 agaaaagtcg ggaaaattgg aaactagcag gcatcacgct tgtgacggcc attataactg
     7321 gctgggcctt ggattctatt ttgatcttag gcatcctgtt agtatcaggc ttactgtttg
     7381 gattttttac agatgatgag gagggcttat gaaagaaacc caaatgctca aaggcgtcct
     7441 agacggctgt gtcttgcagg tcattgcccg acaggaaatc tatggttatg agttggtaca
     7501 agagttgagg aagctgggct ttgaaaacat ggttggaggg acagtctatc ccctccttca
     7561 gaagttagag aaaaatagcc tgatttttag tcagaacaag ccctcgccag atggacccga
     7621 caggaagtat ttctatctta cagaccaggg aaaggcttat ctagaagatt tttggagcca
     7681 gtggacggaa ttggttcaaa aggttcagcg attgaaagga gaataagatg agcaaacaaa
     7741 tggaataccg caagcagatt gaagtgattg aaagccagct gacaaaggaa aacaaggaat
     7801 acatgggccg tatcaatggc tacatgatga ttgcctctgt ctttcacagg caggaagaag
     7861 cagtgactgc ccagctttta tccatttacc aagatgttct ggaagcccaa aaagacgggc
     7921 tttctgcgga ggattttctg ggcaaagaca gcaagcaaat ggcagacgac ctactcagct
     7981 acctccctcc gattggtttc atggaagtcg caaacctgag tggcctcatg ctcatcattt
     8041 acctcggcag ccaatggctg atggattttg cagggacagg caccatttcc ataaactggc
     8101 tgggtttgat ctgcgacgcg gtacttagcc tcctcctccc ctcggggatt tttctcatca
     8161 ttcgcggtct catttaccag accagcaaaa tcaaggtctg ggcaagtttt ctctgcatcc
     8221 cccttctttt tctcctgatt tgcggacttc gtctctgggc aatccccaag gaacctgacc
     8281 tagtcttgac tggatggggg cttctagtac ccctcacctt gctcggtctc gctctgctat
     8341 ttttccaaaa ggaaaagttg gttcgctatg tctttatgcc tagttatctg ttaatgatgg
     8401 ttggcggtgt ggtgaatatg gtcatgaccg ttccagtttg gctcaatctg atgttggtga
     8461 ttcttccagc catggccttt tggattggaa gtgcggtttt gttggtgaga aaggagaagt
     8521 aagatgaaca agcaacagaa aaaattgaac gctattagca gcttgcaggg gaggttgacc
     8581 aaggaaaaca aggcctatat gcgcaagctc catgcttact tgatgttggc atcagccttc
     8641 catgagcagg aggaaagggc gactgaactc ttgctttcca tctaccaaga tgtcctggat
     8701 gcccaagcag atggtcagtc agcagaggat tttttaggca aagacagtaa gcagatggcg
     8761 gatgaactct tgtctgacct accgcccatt cgctggtact atggtttgcg gttaacagga
     8821 ttgatttcct tggtctatat tagctggttt ttcttagatt tgtttggagt gactggtgag
     8881 atgttcctag aatggcgggg cttgctgtgc gatttgcttc tggcgctcat tctacccagc
     8941 tctgcttttt tcatcctaaa gaacctagtc tatgaacggt caaaaatcaa gtcctatttg
     9001 ctgatgggga tgtgggggct tagttttgtg gggctgattg tcttgcgttt ttggattagc
     9061 cgtcagtttt atgaaggagt tcccctgccc ttgtgggctt caatactaat actgataggg
     9121 attgcagttg gaatctggta ctaccgaaag aaatcaccga ttgtttatgt ttttgtacct
     9181 gcctatctac taactgttct gagaggtttt agtcaggtga ctgcgagtca gtttggctac
     9241 ggaccgcagg attggacagg ttggttgcct ctgctcttta tggttcttgc ctttttctca
     9301 acgcttgcag gtacattttt cctattgaaa aaggagaata tgacttagaa acaggatcag
     9361 actatgcttt agagtagtct gattttttgg tataatgaaa ggagcataat taggagaatg
     9421 tattgtgaac gaacaacaac aacgaaaaga attacaccag aaaatttggg ctattgctga
     9481 cgatgtccgt ggagctgtgg acggctggga ctttaaacag tatatacttg gtattttgtt
     9541 ttatcgcttc atttcagaaa atattgcgga ttattttgat caggcagagc atgcggctgg
     9601 ggataaggaa ttttcttatg ctttactgac tgatgaagaa gcagaagagg attttaggca
     9661 ggggactgtt gaagaaaaag gattctttat tttgccctcc caactatttg tcaatgttgt
     9721 agctaaggca agaaccaatg aaaatttgaa tacagatctt gcgactattt ttgaacaaat
     9781 tgaaaaatct gctatggggc acgaatctga aaatgatatt aagggcttgt ttgatgatgt
     9841 ggatacccgt tccaatcgtc taggtggaac cgtagctgaa aaaaataaac gtctggcaga
     9901 tattttgcta ggtatttcag aaattgattt cgcaaatttc caagacaatg aaattgatgc
     9961 ttttggagat gcctatgagt atcttatctc caattatgct tccaatgcag gtaaaagtgg
    10021 gggagaattt tttacaccgc aaacggtatc caaacttctg gctcatctgg taatggttgg
    10081 aaaggacaaa attaataagg tctacgagaa gaagataaag gattggatac acaaacctac
    10141 actttactct cttaaaagtg cttaaaacaa agcctttaag aaaaatcaaa aaccggataa
    10201 aaataatctt tgttctagct aataggaagg ctagattttt tattttaggt gtgtcaattt
    10261 caataataag ggggtcaata aaatcatttg gggtgtgtct tgatattact tgacttattt
    10321 ggcttaaaga gtgatgtata taagccaagg aggttattga agtgagagaa gtcaaacaaa
    10381 taatacaaga cgagatggat tatagctttt gtaaattgt
//
LOCUS       DATUCG010000088        10277 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.090, whole genome shotgun sequence.
ACCESSION   DATUCG010000088 DATUCG010000000
VERSION     DATUCG010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10277
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.090"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(<1..1323)
                     /locus_tag="VCA20_001713"
     CDS             complement(<1..1323)
                     /locus_tag="VCA20_001713"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012948.1,HMM:NF018196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEP1835497.1"
                     /translation="MVKTKAYGLIGAITLAGSFGLFNVSTVEAAEIKSGLQNTPISRT
                     AITSLTAEQQSQIIAGEITEVPMSWINAETGNVQWITSFTLVYKQNGEVVTVPVEPGT
                     VTPPTPTSSPLAKLLPNTGTAASTVITLAGVGLVGFAGWLAFRNKKSGKIFLVSLCVA
                     GGIATSLTALVAEVDFLSIVDTVSIELSSHFSHTAATHEDWEYVGYIPTIAEESVKET
                     TATGSVNVKYVDTEGNEIKDLYNLVVDGIVSTTTTTTTTTDGVASVTEDTVASGLTYD
                     ATTQKPASITYNGKVYELVQVKDGDVEQGLVSEGTTTVTYIYRHVPQVTTDVKTSTTT
                     GSVNVKYVDTEGNEIKDLYNLVVDGIVSTTTTTTTTTDGVASVTEETVASGLTYDATT
                     QKPASITYNGKVYELVQVKDGDVEQGSVTEGTTTVTYIYRYVPQVTTDV"
     gene            1589..1726
                     /locus_tag="VCA20_001714"
                     /pseudo
     CDS             1589..1726
                     /locus_tag="VCA20_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002906470.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1838..2914
                     /locus_tag="VCA20_001715"
     CDS             1838..2914
                     /locus_tag="VCA20_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835498.1"
                     /translation="MKKLLICIVLLSCLVFPFIGWKLYAYQHNAITLTSEADFYLVYP
                     GKVEAFQLNGQDPQLLHTQKMNSEGYFGSGENYILEDRYLVFGNDHQKFIHDNLISID
                     FTDGTVLRKPSKHSTSISGTDGNHFYTAGAYHTLAQFDKQFETRQTHVLNDDFIPNDP
                     VMVDQTSVYLTGFVKDFTQEGPEENVLIVFDKKDFSKQEVFKYDNTIATGEGLLVNGT
                     IYLPNLGKKTSEYTEIEQSSELLTFDTKTKTFSSVTLEHPSPSTLHALKDQKLLLIEH
                     QNGMFSPISFTIYNTQTGQESYHRLDELNPRPYYIDHVRQLDGNRLLLILAGKALIYD
                     WQAKKVLSQTILSEDYVSGVWVND"
     gene            complement(3036..3343)
                     /locus_tag="VCA20_001716"
                     /pseudo
     CDS             complement(3036..3343)
                     /locus_tag="VCA20_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910392.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3432..4895)
                     /locus_tag="VCA20_001717"
     CDS             complement(3432..4895)
                     /locus_tag="VCA20_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEP1835499.1"
                     /translation="MDFGFDILAIMIPIIVVVLFVLLFVFGYVSAKPNEAIVITGLGK
                     PRTLIGRSGFMIPFIEKRSYISIEQFSTDVQTTDFVPTLDFINVKADAVVKVKVGVSD
                     ELLNAAAQNFLNWKTADISASIQDVLEGNLREIIGQMELRDMVNNRQAFAEKVQSNAA
                     PDLAKMGLEIIAFTVQSFTDDNDVIKNLGIDNIVTIQKDAANARAKAEREQAEVRARE
                     DKAANDARVAADLEIAKKQNELAIEQANLKRQSDVQLAQANAAYGIEEQAQRKEIERA
                     TAEANIVKQQKEAEVKAEEVKVREQELSATIRKQAEAEKYARQQAAEADLIERQRKAE
                     AELYETQREAEAQKARAEAAKYAAEQEAAGIEAKGRAEAEAIRLKLEAEAEGLSKKAD
                     AMAKFNDAAVTEMVVNVLPEIAKNIAAPLGNVDKITMYGEGNASKMVGDLMTTMDKTT
                     EGLGLNVRDLISATLTGRAMSTGLLTAQETKEAEGDK"
     gene            complement(4907..5488)
                     /locus_tag="VCA20_001718"
     CDS             complement(4907..5488)
                     /locus_tag="VCA20_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEP1835500.1"
                     /translation="MKKRWLVILIPVLALLFLGMGAVGQYNGLVDSYAEVENAQANVD
                     TQLQRRYDLIPNVVAAVKGAMEHEEEIFTAIAEARAKIGSSQVGTSDYNQAQSQLDSA
                     VSRLLLVAENYPQLTANQQVSDLITELEGTENRILVARKDYNAVATTYNKKIKRFPTS
                     IYANLFGYEKVELFQASTDAATTVPSVDLSDKE"
     gene            complement(5561..6343)
                     /locus_tag="VCA20_001719"
     CDS             complement(5561..6343)
                     /locus_tag="VCA20_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEP1835501.1"
                     /translation="MRKWKKIIIGCFALLTVCLLFAPTLARVNQIPDRPTGTTVVDET
                     QLLSSETIAEIDQLNRSWASTEQALQVGVYVTESLSSDIESLANETFRHWQVGFAGTD
                     NGVLLVVAIADRAFRIETSDNAATVITDVEAKDILESSREFFRQEDYSGGISYIVNSI
                     GDRFYGTSIGKNQLAAIEEGTSEKDDGFWLFLIVILIAILFGIIDKSSRGGGGPGNLL
                     WMLVDDHHHYHNHHSSNSSSSSFGGGSWSGGGGGGGGASSGW"
     gene            complement(6413..6991)
                     /locus_tag="VCA20_001720"
     CDS             complement(6413..6991)
                     /locus_tag="VCA20_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEP1835502.1"
                     /translation="MELTDIRQAIIEDVANRDFTNKGIAPLFQAPSTAKILIIGQAPG
                     LKTQEKGRLFDDASGDNLRKWLGVGRQTFYESGHFAILPMDFYYPGKGKSGDLPPRKD
                     FAPKWHPLIMKQLPQIELTLLIGKYAQDYYLGESHRTVTERVENAGDYLPTYFPLPHP
                     SPRNNIWQAKHPWFVEQTLPILKNNINILLGK"
     gene            complement(6978..7436)
                     /locus_tag="VCA20_001721"
     CDS             complement(6978..7436)
                     /locus_tag="VCA20_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEP1835503.1"
                     /translation="MELKELCFSDMEMVKELFLSVFSQEPWNDDWSDEEQLDCYIGDL
                     LGHSRALCFGLFDQGKLIGLSLGYIRYWYEGTEYRIDELCISRPYQGRGIGQDFLKRI
                     EEQLDRRQIVHILLQTERNLPAYFFYKKCGFRALEEDVTMVKKVGHHGTY"
     gene            complement(7477..8877)
                     /gene="pepV"
                     /locus_tag="VCA20_001722"
     CDS             complement(7477..8877)
                     /gene="pepV"
                     /locus_tag="VCA20_001722"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEP1835504.1"
                     /translation="MTVDFRAEFDKRKDEFLVDLFDLLRINSERDDSQADAQHPFGPG
                     PVRALDKFLEIAQRDGYPTKNVDNYAGHFEFGEGDEVLGIFGHLDVVPAGSGWNTDPY
                     EPQIIDGKLFARGSSDDKGPTMACYYGLKIIKELGLPTSKKVRFIVGTDEESGWADMD
                     YYFEHVGLPLPDFGFSPDAEFPIINGEKGNITAYLHFAGENSGAAKLHSFTGGLRENM
                     VPESATAIISGDLADLDSKLADFTVAYGLNADAETLENGQVQVTVIGKSAHGSTPEEG
                     VNGATYLAKFLSQFVFDGAAKTYLDLAGQVLLEDHDAQKLGVAIYDEQMGALSMNAGV
                     FKFDETSSDNTIALNFRYPKNTNPETIKAGLEKLGVEAVSLSEHGHTPHYCPIDDPMV
                     ATLLSVYEKHTGLKGHEQVIGGGTFGRLLKRGVAYGAMFPGDVNTMHQANEFIEVEQL
                     YRAAAIYAEAIYELIK"
     gene            complement(8978..10183)
                     /gene="sstT"
                     /locus_tag="VCA20_001723"
     CDS             complement(8978..10183)
                     /gene="sstT"
                     /locus_tag="VCA20_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEP1835505.1"
                     /translation="MNKIISVWKKMNLIRKIGIGVVLGVLLGLIAPKITVIALFGSLF
                     VGALKAIAPLLVLTLVAHALSQAPAGQKSNMRTVICLYLFGTFAAAFIAVGASYLFPI
                     KLVLSTTTTTDITPPQGIAEVFQDLLLKIVDNPINALATANYIGVLTWAAVFGLAFRH
                     ASKTTKDLLQSAAEVTSKVVGWIIGLAPFGIMGLVFDTIANNGLTALKDYGLLLLLLV
                     GSMVFVALVVNPLIAFLVMKKNPYPLVFECLRVSGVTAFFTRSSAANIPVNIQLCKRL
                     GVDPDTYSISIPLGATINMAGAAITINILTMAAVHTLGISVDFSSALLLSVVASLSAA
                     GASGVAGGSLLLIPVACSLFGIPNEIAMQVVGVGFVVGVIQDSCETALNSSTDVLFTV
                     VAERSAWKK"
BASE COUNT         2889 a         2356 c         2020 g         3012 t
ORIGIN      
        1 cacatcagtt gtaacttgtg ggacataacg gtagatatag gttactgtcg tcgttccttc
       61 agtaacagag ccttgttcta catctccatc tttaacctga acaagttcat agaccttacc
      121 attgtaagtg atagatgctg gtttttgagt cgtcgcatcg taggtcaaac ctgaagcaac
      181 tgtctcttct gttacgctcg ctacaccatc tgtggtagtc gtagtcgttg tggtggttga
      241 aacgataccg tccactacca agttgtagag gtctttgatt tcattacctt ctgtgtccac
      301 atacttgaca ttgacactac cagtagtagt tgaagtcttc acatcagttg taacttgtgg
      361 gacatgacgg tagatatagg ttacggtcgt tgtaccttca ctaacaaggc cttgttctac
      421 atctccatct ttaacctgaa caagttcata gaccttacca ttgtaagtga tagatgctgg
      481 tttttgagtc gtcgcatcgt aggtcaaacc tgaagcaact gtgtcttctg ttacgctcgc
      541 tacaccatct gtggtagttg tagtcgttgt ggtggttgaa acgataccgt ccactaccaa
      601 gttgtagagg tctttgattt cattaccttc tgtgtccaca tacttgacgt tgacactacc
      661 tgtagcagtc gtctctttaa cggactcttc tgcaatggta ggaatataac caacgtattc
      721 ccaatcttcg tgggttgctg ctgtatgtga aaaatgtgaa gacagttcaa ttgaaactgt
      781 gtctacaatc gacaagaagt caacttctgc tactaatgcg gttagagaag ttgcaattcc
      841 tccagctaca cagagggaaa ctaagaaaat cttacctgac tttttgtttc taaatgctaa
      901 ccaacctgca aaaccaacta aaccaacacc ggctaaagtg atgactgttg aggctgctgt
      961 tcctgtattc ggaagaagct ttgccaatgg tgagctagtt ggagttggag gcgtcactgt
     1021 tccaggttca actggaactg tcacaacttc gccattctgt ttgtaaacaa gggtgaagct
     1081 agttatccac tggacatttc ctgtctcagc attgatccaa gacatcggta cttcagtaat
     1141 ctcccctgca ataatttggg attgttgttc tgctgttagt gaagtaatcg cagtacgact
     1201 gataggggta ttttgaagcc cagatttaat ttctgccgct tcaacagttg agacattaaa
     1261 taagccaaag gatccagcga gtgtgatggc tccaatcaac ccataggcct tcgtttttac
     1321 cattcgctat cttccttcag cttctgcaat ttttgaaaat atatattttc tattcattta
     1381 gggtttcctt ttttttagtt tttcacattg taccaaacta ttttgaaaaa gtaaactaat
     1441 ttgcccccat aagctacttg ttatcgtttt ctatttttcc cttcttcctc agtagcaata
     1501 gccaagctcc acaggtcatt tcgtagagga taaggaaaag gataaagtaa tgcaggaaga
     1561 attctgctgg caggataaaa atgacagttt ggaaatgggc atgcctgttg acttgttaca
     1621 ggttgacctg acccgctgtt ggcagatttt gggagaaatc acaggcgatg cagctccaga
     1681 cgaactcatc acccaactct ttagccaatt ctgtcttgga aaataagaac aaaagtcatg
     1741 taagaaaact tgcatgactt tatttgtata ttaacacaga atttcttgtc taagatgaca
     1801 aatttagata tactaaacag tgaaaggagg cattcccatg aaaaaacttc ttatttgtat
     1861 tgtccttcta tcctgtcttg tctttccatt tatcggctgg aaactatatg cctaccagca
     1921 caatgcaatc acattaacgt cggaagctga tttttacttg gtttatcctg gtaaggtcga
     1981 agcctttcag ctaaatgggc aagatcctca actccttcat acacagaaaa tgaattcaga
     2041 aggctatttt ggctctggtg aaaattacat cctcgaagac cgctacctag tctttggcaa
     2101 tgaccatcag aaatttatcc atgataatct catttccatt gacttcacgg acggaactgt
     2161 tttgcgtaaa cctagcaaac atagtacctc gatcagtggc acagatggaa accatttcta
     2221 tacagcaggt gcttatcata cgcttgcgca atttgataag caatttgaaa cacgtcagac
     2281 ccacgtttta aacgatgact tcatcccaaa tgatccagtc atggtagacc aaacgtcagt
     2341 ctatttaact ggttttgtga aagattttac ccaagaagga cccgaagaaa atgtcttgat
     2401 tgtatttgat aagaaggatt tttcgaaaca agaggtattt aagtacgaca ataccattgc
     2461 cacaggagaa gggcttttgg tcaatggaac catctatctc ccaaatttag gaaaaaaaac
     2521 aagtgagtat acggaaattg aacagtcatc cgaactccta acctttgata caaaaacaaa
     2581 aacattttcg tcagtaactt tagaacaccc ctcaccaagc acactacatg ctctcaaaga
     2641 ccagaagctt ctcctcatcg aacatcaaaa tggtatgttt agcccgatct catttaccat
     2701 ttacaatacc caaacaggtc aagagagcta ccatcgtcta gacgagctta atcctcgtcc
     2761 ttattatatt gaccatgtcc gtcaactaga tggtaacaga ttattattga ttctggctgg
     2821 taaggcactc atctatgact ggcaagcaaa aaaagtttta agccaaacca ttctatctga
     2881 ggattatgtc agcggagtgt gggtaaatga ctgataaaca aaaacgaatc tcaataaaga
     2941 ttgtaaaagt cccaagatga ctctttcttg ggactttcgc atattctatc gaagttgcac
     3001 agcataatca atgtacttac aaatcctcgg aaccactatc taatcgaagt aaacgccatt
     3061 cccaaagcag aaccaaaata atatttccac ctttcttgct ctaaatagcg cttaaacatc
     3121 tttaatttat ccgattgaat cctattcaaa gctagttctt ccaataccca tggataaatt
     3181 ttatatgaaa gatagctaat cttactacct ttttcacaca actccattgc ttccaacaac
     3241 tggaatttca atgctttgct caactttaat tctgaatcag ataatagttc atttattttt
     3301 tgtataaaca acttactttt ttactgtttc taaacatttt catcttcgta gagatttctg
     3361 aaacaatcat gcgatggaaa tgatggtgta aacctactca atcacccatc tcttccaact
     3421 gcttattatt tttatttgtc cccttccgct tcctttgttt cttgggcagt cagtaaacct
     3481 gttgacatgg cacgaccagt tagggttgcg ctgattaaat cacggacatt gagtcctaga
     3541 ccttctgttg tcttgtccat ggtcgtcatc aggtctccca ccatttttga ggcattccct
     3601 tcgccgtaca tggtaatctt atcaacattt ccaagtggtg cagcaatatt cttggcgatt
     3661 tctggaagaa cattaaccac catttctgtc accgctgcat cattaaactt agccattgcg
     3721 tcagctttct tgctgagacc ttctgcctct gcttcgagtt tcaaacggat ggcttctgct
     3781 tcagcacgtc ctttggcctc aatacctgcc gcttcttgct ccgcagcata cttggcagct
     3841 tctgcacgcg ctttttgagc ctccgcttca cgctgcgttt cgtagagttc tgcttctgcc
     3901 ttgcgttgac gttcaatcaa atctgcttct gcagcttgtt gacgagcgta tttttctgcc
     3961 tctgcctgtt tgcggatagt agctgagagc tcttgttcac gaaccttaac ttcctctgct
     4021 tttacttctg cttctttttg ttgcttgaca atattggctt cagcagttgc acgctcgatt
     4081 tctttccgtt gagcttgctc ctcaatgcca taggctgcgt ttgcctgtgc cagttggaca
     4141 tcggactgac gttttagatt agcttgctca atagctagtt cattctgttt cttggcgatt
     4201 tctaagtctg cagcaacacg cgcatcatta gccgccttat cctcacgggc acggacttct
     4261 gcttgttccc gctctgcttt ggcacgagca tttgcggcat ccttctggat ggtcacaata
     4321 ttgtcaatac ccaggttttt aatgacatcg ttgtcatctg tgaaggattg aaccgtaaat
     4381 gcaatgattt ccagccccat tttagccaag tcaggagccg cgttggattg gactttttct
     4441 gcaaaagcct ggcggttatt aaccatgtct cgcagttcca tctggccaat gatttcacgc
     4501 agatttcctt ccaagacatc ttgaatagag gcagaaatat ctgcagtttt ccagttaagg
     4561 aagttttgag ctgccgcatt gagcaattca tcggataccc caacctttac ttttacgacc
     4621 gcatcagctt ttacgttgat aaaatcaaga gttggtacaa aatctgtggt ctgcacatct
     4681 gttgaaaact gctcaataga gatataggag cgtttttcga taaagggaat cataaaacca
     4741 gaacgaccaa ttaaagtccg aggtttgccc aagccggtaa tgacaatcgc ttcattaggc
     4801 ttagcagata cataaccaaa tacaaacaat aaaacaaata gtacaacaac aataattggt
     4861 atcataattg ccaatatatc aaatccaaag tccatattta tctcctttat tccttatcac
     4921 tcaaatccac acttggtaca gtcgtcgctg catctgtgct agcttggaaa agctcgactt
     4981 tttcatagcc aaataagtta gcatagatgg aggtcgggaa ccgcttgatt tttttattgt
     5041 aggtagttgc caccgcatta taatctttac gggctaccag tatccgattt tcagtccctt
     5101 ctagctcggt aatgaggtca gatacctgtt ggttggctgt caattgcgga taattctcag
     5161 ccactagcaa caatctagat actgcagaat ccagctgact ttgtgcctga ttgtagtcgc
     5221 tggttcctac ttgactggaa ccgattttag cacgcgcttc tgcaatagca gtaaatattt
     5281 cttcctcgtg ttccatggcg cctttgacag ctgcgactac atttggtatc aagtcatagc
     5341 gacgttgcaa ctgtgtgtct acattggctt gcgcattttc aacctcagca taactgtcaa
     5401 ctaagccatt gtattgccca actgctccca ttcctaaaaa cagcagagct aggactggaa
     5461 tgaggataac caaccatctc tttttcataa gatttccttt ctaactacga ttaaacattc
     5521 gtaaaaaagc agacaactat cagaagcctt atacgatgcc ttaccatcca gaagaggctc
     5581 cgccaccgcc accgccacca cctgaccaac tacctccgcc aaaactagac gatgaggaat
     5641 tagacgagtg atgattgtga taatgatgat gatcatccac caacatccag agtagattac
     5701 ctggtccgcc accaccacga ctagatttgt caatgatacc aaataggata gcgatcagaa
     5761 taactatgag aaacagccag aagccgtcat ccttctcact tgtaccttct tctatagcag
     5821 ctaactgatt tttaccaatg cttgtaccat aaaacctatc cccaatggag ttgacgatat
     5881 aggaaattcc tccactgtag tcttcttgac ggaaaaactc cctagaactc tccaggatat
     5941 cctttgcttc cacatccgtg atgactgtgg cagcattgtc tgaggtttct atccgaaatg
     6001 ctctgtcagc tatggcaacc actaaaagaa ctccgttgtc cgttcctgca aaaccgacct
     6061 gccagtgacg aaaggtttca ttagccaaac tttcaatatc actggacaga ctttctgtta
     6121 cataaacacc aacctgtaaa gcttgctcgg tgcttgccca acttcgattg agttgatcaa
     6181 tttctgcaat ggtctcactc gagagtaact gcgtttcatc cactaccgtt gtacctgtgg
     6241 gtcggtctgg gatctgattt actctagcta gtgtgggagc aaatagcaag caaaccgtta
     6301 gaagagcgaa acagccgatg ataatttttt tccattttct catcagaaca cctccttcga
     6361 tgaacaaaga taagaaaaat ttattaggta tatatacaat tatatcatat tatcattttc
     6421 ccaacaatat atttatgttg tttttcagta tcggtagagt ctgttcaaca aaccaaggat
     6481 gtttagcttg ccagatattg ttccttggcg atggatgagg taggggaaaa taggtgggta
     6541 ggtaatcacc tgcattctct actctctcgg tcacagtcct gtgggattct cctaaatagt
     6601 agtcctgggc atacttacca atcaacaagg tcagctcaat ctggggtagt tgtttcataa
     6661 tgagtgggtg ccatttgggc gcaaaatcct tgcgaggcgg caaatcccca gacttccctt
     6721 ttcctggata atagaaatcc ataggaagaa tggcaaaatg ccccgattca taaaaagtct
     6781 gtcgacccac gcccaaccat ttacgaagat tgtctccact ggcgtcgtca aataaacgcc
     6841 ctttttcctg ggttttcaag ccaggtgcct gtccaataat gagaattttt gcggtcgaag
     6901 gggcttgaaa aaggggagct attcccttgt tcgtaaagtc tcggttagca acatcctcaa
     6961 tgatggcttg acgaatatca gtaagttcca tgatgtccta ctttcttgac catggtcaca
     7021 tcttcttcta aggctcggaa accgcatttt ttgtagaaaa aatatgctgg aaggttgcgt
     7081 tcggtttgca gaagaatatg aacaatctgt cttctatcca actgctcctc aattcttttt
     7141 aggaaatctt gtccgattcc tcgcccttgg tagggacgag aaatacatag ctcatcaatt
     7201 cgatactcag ttccctcata ccaatagcga atgtaaccaa gtgacagacc tatcaatttt
     7261 ccctggtcaa acaagccaaa acagagtgct cttgagtgtc ccagtaaatc gcctatgtag
     7321 caatctaatt gttcctcatc tgaccaatca tcattccaag gttcttggct gaaaacagac
     7381 aaaaataatt ctttaaccat ttccatgtct gaaaaacaaa gttctttcag ttccatattt
     7441 cctcctaaaa aagacgggca tccccgtctt catgtattac ttaatgagtt cgtagatagc
     7501 ttctgcataa atagcagctg cacggtagag ttgctcaact tcgataaatt cgttggcttg
     7561 gtgcatggtg ttgacatctc ctgggaacat agcaccataa gcgacaccac gtttgagcag
     7621 gcgtccaaat gttccgccac cgatcacttg ttcgtgacct ttcaagcctg tgtgtttttc
     7681 ataaacagac aagagggttg cgaccattgg gtcatcgatt gggcagtaat gtggggtatg
     7741 gccatgctca gacagactaa cagcttccac acccagtttt tccaaaccag ccttgatggt
     7801 ttctgggttg gtattttttg gataacggaa gttgagggca atggtgttat cagatgaggt
     7861 ttcatcaaac ttaaagacac cagcgttcat cgaaagagct cccatttgct catcgtagat
     7921 ggctacgccg agtttttggg catcgtggtc ttccagaagg acttgtcctg ccaagtcaag
     7981 ataggttttg gctgctccgt caaagacgaa ttggctgagg aattttgcca aataggttgc
     8041 tccgttgaca ccttcttctg gggttgaacc gtgggctgat tttccgatga cggtgacttg
     8101 aacttgacca ttttcaagag tttccgcgtc agcatttaag ccgtaagcga ctgtgaaatc
     8161 tgccaacttg ctgtccagat ctgctaggtc cccagagata atagctgtcg cagattcagg
     8221 caccatgttc tcacgcaaac caccagtgaa ggaatgcagt ttagcagctc cgctattttc
     8281 ccccgcaaaa tgcaaataag ctgtgatatt gcccttttcc ccgttgataa tcgggaactc
     8341 agcatctgga gaaaagccga aatccggcaa aggaaggccg acgtgctcga agtagtaatc
     8401 catgtccgcc cagcctgact cttcatcggt accgacgatg aaacggactt ttttagaggt
     8461 cggaaggccc aactccttga tgattttcaa gccgtagtaa caagccatgg tcggtccctt
     8521 atcgtcagag gagccgcggg caaagagctt accgtcgatg atttgcggct catatgggtc
     8581 agtgttccaa ccgcttcctg ctggcaccac gtccaagtga ccgaaaatac caaggacttc
     8641 gtcgccttca ccaaactcaa agtgacctgc gtagttgtca acgttcttgg tcggatagcc
     8701 atcacgctga gcaatttcta gaaacttgtc caaggcacgc actggtcctg gcccaaatgg
     8761 gtgttgggca tctgcctggc tgtcgtcacg ctcagaattg atgcggagga ggtcaaagag
     8821 gtccactaga aactcatctt tgcgtttgtc aaactctgct ctaaaatcta ctgtcatatg
     8881 ttctcctttt cagtatcaaa gtaagtctat tttatcacaa attgaaagag atttcagaag
     8941 taaagcgaag aaaaaaagtt ggtcacccaa cttctttcta ttttttccag gcagagcgtt
     9001 ctgctacaac tgtgaacaaa acatcggtcg atgaattgag ggctgtttca caagagtcct
     9061 gaatcactcc gaccacaaat ccgactccaa caacctgcat ggcgatttca tttggaatac
     9121 caaaaagact acatgctact gggatgagga gaagcgaacc acctgctaca cctgatgcac
     9181 ctgcagctga tagtgatgca acaacggata aaagaagagc tgagctgaaa tcaacactaa
     9241 tgccaagtgt atgaacagct gccattgtta aaatattaat cgtaatagcc gcgccagcca
     9301 tattaattgt ggcaccgagc ggaatggata ttgagtatgt atctggatcc actcctaaac
     9361 gtttgcagag ctgtatattg acagggatat tagctgctga acttcttgta aaaaatgccg
     9421 taaccccact aacccgaaga cattcaaata ccagcgggta cggatttttc ttcattacaa
     9481 gaaaagcaat caagggattg acaacaagcg cgacaaaaac catgcttcca actaagagta
     9541 agagcagtaa accatagtct tttaaagctg tcagaccatt atttgcaatg gtatcgaaaa
     9601 ccaagcccat gataccaaat ggtgccagac caataatcca gcctacaact ttagaagtca
     9661 cttctgccgc agattgcaat aaatctttgg ttgtcttact tgcatgacga aaggccaagc
     9721 caaacactgc cgcccatgtc aaaacaccaa tataattagc cgttgcaaga gcattgatcg
     9781 gattgtcgac tattttcaat aacaaatctt ggaatacttc agcaatccct tgtggaggag
     9841 taatgtccgt agtcgttgta gtagaaagaa ccagtttaat agggaataaa tagcttgctc
     9901 ctacagcaat gaaggccgcc gcaaaagtac caaatagata caagcagata actgtcctca
     9961 tattcgattt ttgaccagca ggagcttgtg atagagcatg tgcaactagt gtcagaacca
    10021 aaagaggggc tatagctttc aatgctccta caaatagact accaaagaga gcaatgaccg
    10081 ttatttttgg agcaattaag cctagtaaca ctcccaaaac aacaccaatt ccgatttttc
    10141 ggattaaatt catttttttc catacagata ttattttatt cactcattta cctccaaaca
    10201 aacatttttc aatcatagca gaaaaacgaa ggttggtcaa tagcataaaa aatgagatat
    10261 tagacttgtt cggtaac
//
LOCUS       DATUCG010000089        10238 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.091, whole genome shotgun sequence.
ACCESSION   DATUCG010000089 DATUCG010000000
VERSION     DATUCG010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10238
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.091"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(50..9592)
                     /locus_tag="VCA20_001724"
     CDS             complement(50..9592)
                     /locus_tag="VCA20_001724"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012906.1,HMM:NF012956.1,HMM:NF014850.1,
                     HMM:NF014851.1,HMM:NF019170.1,HMM:NF027673.1,
                     HMM:NF037850.1,HMM:TIGR01168.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylglucosaminidase domain-containing
                     protein"
                     /protein_id="HEP1835506.1"
                     /translation="MKRSSKDLFDKRYSYSIRRLSVGTASVVVGCMLFGSQMVQANTV
                     EASDPSLELVTDETELSVIPEEEAVEETPAVLSEGEEVGQDGSESVTEPAELEAVAAE
                     PMLAPATDLPQINNGEGGESREATVQSQEQVVAPASWTTIHHYSQVDQRVNFDQAWKF
                     SLGETAGASAKQFDDSSWQVLDLPHDFSLIQDYTQSGEAESGYKPGGTGWYRKAFTAS
                     EEVAAGRVELQFDGAYMETEVFINGTSLGVHPNGYSPFTFDLTGHVRANEENIIAVKV
                     VNPVPSSRWYSGSGIYRSVHLNMTPKVHLAEYGVVVTSPNLKESKTGQGTASRVEVVT
                     ALENAGSQSAAVIVRTSLFERLADGQVGTKVAEKTSSLTTISSQNSSKIRTEFDINNL
                     KLWSVSNPHLYIVRTEILDGETVVQSRDQETGFRYIEFDNVSGFKLNGEKIKLQGVSM
                     HHDQGGLGARAYYDAIERQFEILKGMGVNAVRVTHNPAARAMKDIANRMGMLLIDEAF
                     DTWERPKNSNVNDYSRFFNRTIGATATNLVGARAQDQQWSEFHVKQMVKSGINDPSII
                     MWSTGNEVLEGISGSASNYVSVINRLIGWINEVDPTRPATIGDNKLKHGDQIAVGMGD
                     ALNNATVRGIVGYNYADGRKYDESRASHPNWIIYGSETASSVNSRGVYNIKGNASRSD
                     KQRTSYDQSHVNWGHTASQAWYDVITRDFVAGEFVWTGFDYLGEPTPWNGVGPGTQHG
                     WPAPKSSYFGIVDTAGFPKDSYYFYRSQWKQDDTTLHVLPAWNDAVLQKDSNNRVEVV
                     VYSNAAKVQLVHIAADGTQRDLGTKEFTEVTTDTGYKYKIYNGADKSSTPHKNLYLTW
                     QVDYQPGTIKAIAYDAQGNVIEQTVGSSQHSTFGVASKLLAKVAKVDQAVTDHSLAYI
                     EIDVVDTNNNIVVNAANPVSISVSGPAKLVAMDNGNAMDHQSYQADNRKAFAGKVLAI
                     VQMTGASGTVSVTASADGFTASTVAFEVTGQADQTANKAKEYKLSKNIYIKRGAEVNL
                     PTSVIVRFQDGSEVEKAVSFDKAAIREKLATADTVTARARVADLDLDVSLLISVIDQV
                     AAIKNVATAVEIGSPLVLPQTVEAFLADGTLLSSQFPVTWTQPSDDQLRTEGVIEVRG
                     VANVLGDQLPVTAKVRVGNRAITIGGNVAPKATRIEQNVPQNLQSDTLMAIVDGGLQA
                     SANNDGGANETIWSNYDAAQADRKTATLSFVYDTAQNIAEVSIYYHRDNWSLRQPKSV
                     TFSYQTSSSAEATVAAHRVTETKPVNGLTKVTYRLDRSVSAEIFKVTVENSDEVLSGR
                     KPSVGIVELELKTALEAFVKSSDASINEIQIGNQVLRGTSITNNMTVSGRGDITARNT
                     ANNVGVTVLRTADNTVKIVTSSEDGSQSETYTLTFVPDSPTTEGRNYIERSEMTLTAG
                     SVENRASEAVDNAKDFNLNSHWHSTWSGTDISNLWLTVDTGRVRQLNGLAYRHRQDGS
                     PNGKVAEYEILVSSDNRTWTSVKTGRFDESSLDWQEVVFEPVNGQYIKLQAVSTLGNS
                     GRNRFMSASEIRVSEHLGQVETVALTSDHVRLASQEYAYTGQALIPKPIVRVNGVNLV
                     ENQDYRLDYSDNIGQVGTSSQASVRVTGLGRYTGVVSLNFTIRAGQVETISDGTVETA
                     DRFDATRPASAEVIAAALASEDYLNKNYQVFPTPQTVTYGDGLVALNGRVNLVIGQGV
                     DIYTRNRLKDTLQAHQISYATSTSAVEGATNIYIGIHGQAGLAQSHQANGGRTAQLFD
                     KIDAYRLVVKDNAISVIGKDTDAVFFGLTTLKHMLNDSPAPVLREVDVEDFADIKNRG
                     FIEGYYGNPWSTADRMELMRYGGDLKLTQYFFAPKDDEYHNSRWRELYPEDKLEEIRQ
                     MARVGNQNKTRYVWTLHPFMHNSVRFGDDNHYREDLEVIKRKFTQLMGVGVREFGILA
                     DDARHPAGGFASYNRLMADLTQWLESQQAQYSGLRKEMIFVPHEYWGDGDEAELRSLN
                     ENLPESASLTVTGGRIWGQVSNQFLTTLSTNLSQGGKTYRPVQLWINWPCTDNSKEHL
                     ILGGGEKFLQPNVDASLIGGIMLNPMQQSEPSKIALFSAAEYTWNIWESEEAAKRVND
                     IAFNFVETGRFTDSETSLAFRELGKHMINQNMDGRVVKLEESLELAPKLTAFRTKLEA
                     GEDLTEDRRQLRAEFAKLKAAAQLYKESGNELMRNQIHYWLDNTIDQMNALDALLTAT
                     EFVGSEQADQLWNNYYQGLKLYEQSKGYKFWYVSQYEKAELGVQHIRPFILHLLEYLA
                     GQVESELYPDRIKSRLITNRTGAEGSVANILDGDLSTQLLFKNPNTISTGDYVGLEFN
                     KTIDLDSLTFAMGAVSNLRDTFSAAAIQYQTEDGEWLPIPGHTYTGSEALLTFSNLGL
                     KARAVRMIATSDKTNTWFGIRDIAVNKPLEASRQTSVAITHSTNLVYKGQTSEAQIKD
                     GNDATEAMFANADTAGSQRDTTPADAWVRLDLGQVKPISKVRLVQGTGDKLNKGVLEY
                     SVNGTDWTNLTSLSGEQLKEVSQEFEAQYIRVRNTENLPKWWRIKDFSVETNEGHRDM
                     VETSIASLENSPVFETLGSYRLPLPATTQLAPGDFIGIKLDRLHELESLQATGSQLDL
                     VYSPNAVEWYQLDQLSEQPLARYIRLINKGQTSQNLGSSELLVETKEIHPDKLKSTTL
                     GINQVYGNNDVRRIKNLGQLFDGVYNNFVEFADVPRQGGEIILELGVQRPIRKIRAYI
                     QDGTKNYLRDGKIQVSQNGQDWVDIVTVGDGQANASRDDSLGDGWTHDSNLPGNRYIE
                     GSLETPVNAKYLRIYFTANYDYRFVGFTEIVINDGEFVQNINDPTVTGDGGEAAKNLY
                     TNIVDRKVLSSYKPAKDQGHLVYHMSEMTGVNHVKLISSLPAGSSVQLRARVIRQGQG
                     ADPEWVALGSVINSLQTFVLGQDVAHLLDLELSWTDGRPEFYELSTYRADIVQETPIE
                     DQPPTGGETSQPDQPPTGGEPSQPGSDEWVWTVKPESGLLSLTYEKPRLDILERERGY
                     SRQVLDNPDLPVGVTRLVQVGKVGKERVFVQLVTENGHVLSQRIIHTMVLEEAQDEIL
                     EVGRKPLAPQAASESATVQEVEVPVSQAQLPETGSQDQSYLLTGLSVLATLGLVARAK
                     RREE"
BASE COUNT         2550 a         2558 c         2256 g         2874 t
ORIGIN      
        1 ctagagtctg atcttctttt gcatcgggaa gaaattttgg gctgccccct tattcttctc
       61 tgcgttttgc acgcgcaacc aagccaagtg tcgcaaggac actgagtcct gttaagagat
      121 aggactggtc ttggctgcca gtttctggca actgagcttg ggaaacaggt acttcaactt
      181 cttgaacggt agctgattcg ctagcagctt gtggggctag cggttttcta ccaacttcta
      241 agatttcatc ctgtgcttct tccagtacca tggtatggat aatgcgttgg gaaagaacat
      301 gcccattctc agtgactagc tgaacaaaga cacgctcttt acctacttta cctacctgta
      361 ccaggcgcgt cactccgact ggcaagtcag gattgtcaag cacttgacga ctatagcctc
      421 gttctctttc aagaatatcc aggcgtggtt tttcataagt cagagatagg agaccacttt
      481 caggcttcac agtccatacc cactcatctg aacctggttg acttggctca ccacctgttg
      541 gtggttggtc tggctggctt gtctcaccac ctgttggtgg ttgatcttca attggagttt
      601 cttgaacaat atccgcacga taagtagata actcatagaa ctctggacgc ccatcagtcc
      661 atgacagctc aagatccagc aagtgagcca catcttgtcc cagaacaaag gtttgcagac
      721 tgttgattac agaccccaac gctacccatt caggatcagc cccttgacct tggcgaatca
      781 cacgagctcg taactggaca ctggatcctg ccggaagact agaaatcaat ttcacatggt
      841 tgacccctgt catttcagac atgtggtaga cgagatgtcc ctgatcctta gccggcttgt
      901 aggagctgag cactttgcga tccactatgt tggtgtagag atttttggct gcttcaccac
      961 catctcccgt cacagttggg tcattgatgt tttggacaaa ttcaccatcg ttgatgacaa
     1021 tttctgtgaa gcctacgaag cggtaatcgt agttggcagt gaaataaata cgtagatatt
     1081 tcgcattgac aggtgtttcc aggctacctt cgatataacg atttcctggc aggttggaat
     1141 catgtgtcca accgtctccc agagaatcat ccctcgaagc attggcctga ccatctccga
     1201 ctgtcacaat atctacccaa tcctgaccgt tttggctgac ttgaatcttg ccatcacgga
     1261 ggtagttctt ggtgccatct tggatatagg cacggatctt acgaattggt cgttgcacac
     1321 ctaattccag gataatttca ccaccctggc gtggaacatc tgcgaattcg acaaagttgt
     1381 tgtagactcc atcaaagagt tgacctaggt tcttgatccg acgaacatca ttgttaccat
     1441 aaacttggtt aatgcccaag gtagttgatt tcaacttatc tgggtggatt tccttagttt
     1501 caaccaacaa ttctgaacta ccaagatttt gactggtctg ccccttgttg atcagtcgga
     1561 tatagcgtgc caaaggttgc tcggacaact ggtcaagctg gtaccattca actgcgtttg
     1621 gtgaatatac caaatccaat tgactacctg ttgcttgcaa ggattccaac tcgtggaggc
     1681 ggtctaactt gataccaata aagtcgcctg gtgctagctg agtagttgca ggcaatggca
     1741 gacgatagga accaagtgtc tcaaagactg gactattttc cagactggcg atacttgttt
     1801 caaccatgtc tctgtggccc tcattggtct ctactgagaa gtccttgata cgccaccatt
     1861 ttggcaggtt ttccgtgttg cgtacacgga tgtattgggc ttcaaattct tgactgactt
     1921 ctttaagctg ctcaccagaa agggaagtaa gattggtcca gtcagttccg ttgacagagt
     1981 attccaagac acctttgttg agtttgtccc ctgttccctg taccaaacgg accttgctga
     2041 ttggcttcac ttggcctagg tcgagccgta cccaagcatc ggcaggtgtg gtatctcgtt
     2101 gagaaccagc agtatcagca ttggcaaaca ttgcttctgt cgcatcattg ccatctttga
     2161 tttgtgcttc actggtttgt cccttgtaaa ccaaattagt actgtgggta atggccacac
     2221 ttgtctggcg acttgcttct aatggcttgt tgacagcaat atcacgaata ccaaaccaag
     2281 tattggtctt atcgcttgtc gcaatcatcc gcaccgctct ggccttcaga cccaaatttg
     2341 agaaggtcaa gagagcttca ctacctgtgt aagtatgacc tgggattggc agccattctc
     2401 cgtcttctgt ctggtattgg atagctgcgg ctgagaaggt gtcacgcagg ttgctgacag
     2461 ctcccatagc aaaggtcaaa ctgtccaaat cgatggtctt gttgaattcc aagccaacat
     2521 agtcacctgt cgatatggtg tttggattct tgaagaggag ttgagtcgat aggtcaccgt
     2581 ctaggatgtt ggctacactt ccttctgctc ctgtgcggtt ggtgatcaag cgactcttga
     2641 tgcggtctgg atagagctct gattcgacct ggcccgccaa atactccaga aggtgcaaga
     2701 taaatgggcg aatatgctgt acaccaagtt ctgctttttc atattggctc acataccaga
     2761 atttataccc tttcgactgc tcatagagtt ttaatccttg gtagtaattg ttccacaatt
     2821 ggtctgcctg ctcgcttcct acaaactcgg tagctgtcaa aagagcatct aaggcattca
     2881 tctggtcgat ggtattatcc agccaataat gaatctggtt gcgcatcaac tcgttaccag
     2941 actccttgta gagttgggct gcggccttca acttagcaaa ttccgcccgt aattggcgac
     3001 ggtcttctgt caaatcctct cccgcttcca acttggtacg gaaggcagtc agtttcggtg
     3061 ccaattcaag ggattcttcc aatttcacca cacggccatc catattttgg ttgatcatgt
     3121 gtttgcccag ttctctaaag gcaagggagg tctctgaatc agtaaagcgg cctgtttcaa
     3181 caaagttaaa ggcaatgtca ttgacacgtt tggcagcttc ttctgactcc caaatattcc
     3241 atgtgtattc agcagcagag aagagggcga tctttgatgg ctctgattgc tgcatagggt
     3301 tgagcatgat accgccaatt aggctagcat cgacatttgg ttgtaggaat ttttcaccgc
     3361 caccaagaat caagtgctct ttggagttat ctgtacatgg ccagttgatc cagagttgga
     3421 cagggcgata ggtcttgcca ccctgactta aattggtgct caaggttgtc aggaactggt
     3481 tggacacttg tccccatata cggccacctg tcactgtcag gctagcactt tcaggaagat
     3541 tttcattgag cgaacgcaat tctgcctcgt ctccgtcccc ccagtactca tgcggtacaa
     3601 agatcatctc cttgcgcaaa ccagaatact gagcctgctg gctttccagc cattgagtca
     3661 aatctgccat gaggcggttg tagcttgcaa atccacctgc tggatgacgg gcatcgtctg
     3721 ccaggatacc gaattctctc actccaactc ccatcaattg ggtaaatttc cgcttgataa
     3781 cttcgagatc ttcacgatag tggttgtcgt caccaaaacg tacgctatta tgcatgaatg
     3841 gatggagggt ccagacatag cgggtcttgt tttgattccc cacacgcgcc atctgacgaa
     3901 tttcttctag cttgtcttct ggatagagtt cacgccagcg actattgtgg tattcatcat
     3961 cttttggtgc aaagaaatac tgggtcagct tgaggtcacc accgtagcgc atcaattcca
     4021 tgcggtcagc tgtactccaa gggttgccat aatagccttc gatgaagcca cggtttttaa
     4081 tgtctgcaaa gtcttctaca tcaacttcac gcagaactgg agccggactg tcattgagca
     4141 tgtgcttcaa ggttgtcaaa ccaaagaaga ctgcatctgt gtccttacca atgactgaaa
     4201 tagcattgtc tttgaccact aaacgatagg catcaatctt atcaaagagc tgagccgtac
     4261 gtcctccatt agcctgatgg ctttgggcca aaccagcctg accatgaata ccaatgtaga
     4321 tattggttgc accttccacc gcacttgtgc tagttgcata cgaaatctgg tgggcctgca
     4381 aggtatcttt gagacgatta cgggtataaa tatccactcc ttgaccaatg accaggttga
     4441 cacggccgtt tagggctacc agtccatcac cataggtcac cgtttgaggg gtaggaaaaa
     4501 cttggtagtt cttattgaga tagtcctcgc tcgccaaggc tgccgcaatc acttctgcac
     4561 tagccgggcg agtcgcatcg aaccgatccg cagtctcaac cgttccatca gaaatcgttt
     4621 ctacctgacc tgctcgaatg gtaaagttaa gggagacaac tccagtatag cggcccaaac
     4681 cggtcactcg gacacttgcc tgactgcttg tgccaacttg gccgatattg tcactataat
     4741 cgagacggta gtcttgattt tcaaccagat tcactccgtt gactcttact attggctttg
     4801 gtatcaaggc ttgacctgta taggcatatt cttgacttgc tagtcggaca tggtcacttg
     4861 tcagagcaac agtctccacc tgacccaagt gttcgctgac acggatctca ctcgcagaca
     4921 tgaagcgatt gcgaccacta tttcccaagg tactgactgc ctggagtttg atgtattgac
     4981 cattaaccgg ttcaaaaact acctcttgcc aatccagact tgattcatca aatcgacctg
     5041 tcttaacaga agtccaagtt cgattatccg aacttaccaa aatttcatac tcagctacct
     5101 tgccgtttgg acttccatcc tgacgatggc ggtaagccag accattgagc tggcgaactc
     5161 ggcctgtatc aaccgttaac cacaggttac tgatatctgt tcctgaccag gtagagtgcc
     5221 aatggctatt caagttaaag tctttggcat tgtccaccgc ttcgctggct ctattttcca
     5281 cagaacccgc agtaagggtc atctctgacc gctcaatgta gttacggcct tctgttgtcg
     5341 gactatctgg aacaaaggta agagtatagg tttctgactg gctaccgtcc tcagaagagg
     5401 taacaatttt caccgtatta tcagctgttc gcagaacggt cactccgaca ttgttggcag
     5461 tattacgagc tgtgatatcc ccacgacctg atactgtcat gttattggtg atggaggtac
     5521 cgcggaggac ttggttgcca atttgaatct cattgatgct ggcatcactc gacttgacga
     5581 atgcctcaag ggccgtcttc aattccaact caacaatacc gacagacggt ttgcgaccag
     5641 acaggacctc gtcagaattt tcaactgtca ccttaaagat ttcagctgaa actgaccgat
     5701 ccaagcgata ggttaccttg gtcaagccgt tgactggttt tgtctctgtc acacgatgag
     5761 cggcaactgt cgcttctgcg ctagaacttg tctgataaga gaaagtcact gacttaggct
     5821 gacgaagaga ccagttgtct cggtggtagt agatggacac ttctgcgatg ttttgcgcag
     5881 tatcatatac aaagctaagc gtcgctgttt tgcggtctgc ttgggcggca tcgtaattgg
     5941 accagatggt ttcgtttgct ccaccatcgt tgttggcaga tgcctgcaaa ccaccgtcca
     6001 caatagccat cagggtatcc gactgaagat tttgcggaac attctgctcg atgcgggttg
     6061 cttttggtgc aacattacca ccgatagtga tcgcacggtt gcccacacgt accttagcag
     6121 ttactggcag ttggtcgccc aaaacatttg caactccacg tacctcgatg accccttctg
     6181 ttcgcaattg gtcgtcactt ggttgggtcc aagtcaccgg gaattgcgat gagagtaagg
     6241 taccatctgc caagaaggct tcgaccgttt gtggtaaaac cagaggtgat ccgatttcaa
     6301 ccgcggtggc tacattttta attgccgcaa cctgatcgat gacagatatc agcaaagaaa
     6361 catccaaatc cagatctgca acacgagcac gagctgtcac cgtgtctgct gttgccaatt
     6421 tctctcgaat agcagcctta tcaaagctga cagccttctc gacctcgcta ccatcttgga
     6481 agcggactat cacgcttgtt ggcagattca cttctgcccc acgtttgata taaatatttt
     6541 tagataattt gtattcctta gccttgtttg ccgtttggtc tgcctgacct gtcacttcaa
     6601 atgcgaccgt tgaagcagta aaaccatctg cactcgcggt cacggaaacc gtaccgctcg
     6661 cacccgtcat ctggacaata gccaagacct taccagcaaa tgccttgcgg ttatctgcct
     6721 gataactttg atgatccata gcatttccgt tatccatggc taccaactta gctggtccgc
     6781 tgacagaaat ggatacaggg ttggcagcat tgacaacgat gttgttattg gtatcaacaa
     6841 catcaatctc gatataagct agagaatggt ctgtcacagc ttgatccacc ttggcaacct
     6901 tggcaaggag tttgctggct acaccaaagg ttgaatgctg actgctaccg acggtctgtt
     6961 caatcacatt tccctgagca tcataggcta tggccttgat ggtgcctggc tggtagtcaa
     7021 cctgccaggt cagatagaga ttcttatgag gggtgctact cttgtctgcc ccgttgtaaa
     7081 ttttgtactt gtaacccgta tctgtcgtaa cttctgtgaa ttccttggtt cctaaatcac
     7141 gctgtgtgcc atctgcagca atgtgaacca actgcacctt ggctgcattg gaatagacga
     7201 cgacttcaac acggttgttg ctatcttttt gtaaaacagc atcattccaa gcaggaagga
     7261 catggagcgt cgtgtcgtct tgtttccatt ggctccggta gaaataatag gaatctttag
     7321 ggaagcccgc cgtatcgaca ataccaaaat aagaactctt tggcgctggc caaccatgtt
     7381 gggtacctgg ccctactcca ttccacggag taggttctcc aaggtagtca aaaccagtcc
     7441 atacaaactc accggcgaca aagtcacgag tgataacatc ataccatgcc tgactagctg
     7501 tgtgccccca gttaacatga ctctggtcat aggacgtccg ctgcttgtct gaacgactgg
     7561 cgttgccttt gatattgtag accccacggc tattgacaga cgaagctgtt tctgaaccat
     7621 agataatcca gttagggtgg ctagctctac tctcgtcata ctttctacca tcagcatagt
     7681 tataaccgac aataccacgc accgttgcat tgtttagggc atcacccata ccaaccgcga
     7741 tctgatcacc atgcttaagt ttgttgtcac cgatagtggc aggacgggtt gggtcaacct
     7801 cattgatcca gccaatcagt cgattgatga ccgaaacata attgctggca ctaccgctaa
     7861 tcccttccag aacttcgttt cctgttgacc acatgatgat ggacggatcg ttgatcccac
     7921 tcttgaccat ctgtttcaca tggaattcag accattgctg atcttgtgca cgagctccga
     7981 ctagattggt tgctgttgcc ccaattgtac gattaaagaa acgagaatag tcattaacgt
     8041 tggagttttt tggacgttcc caggtatcaa acgcctcatc aatcaagagc atacccatgc
     8101 gattggcaat gtctttcatg gcacgagcgg ctgggttatg ggtcacccga accgcattga
     8161 ctcccatccc tttgagaatc tcgaattggc gttcaatggc atcgtaatag gcacgagctc
     8221 ctaaaccgcc ctggtcatgg tgcatggaaa ccccttgcag tttgattttc tcaccattta
     8281 acttaaagcc agaaacattg tcgaattcaa tataacggaa ccctgtctcc tgatctcgac
     8341 tctgtactac tgtttcacca tcaaggattt ccgtccgtac gatataaaga tgtgggttgc
     8401 tgacagacca aagtttgagg ttgttaatat caaactccgt acgaatcttg ctactgtttt
     8461 ggctagaaat agtcgttaga ctgctggttt tttcagctac ctttgttccg acctggccat
     8521 ctgccaatcg ttcaaagagg ctggttctca cgataacagc tgcagactga ctaccagcat
     8581 tttctaaagc ggtgacaact tctacccgac tagctgtccc ctgaccagtc ttcgactcct
     8641 tgaggttggg actggtcaca accactccgt actccgccaa atgaacctta ggtgtcatgt
     8701 tgagatggac agaacgatag atcccgcttc ctgaatacca acgagaactt ggaacagggt
     8761 taacaacctt gacagcaatg atattttctt catttgcccg aacatgacct gtcaaatcaa
     8821 aggtaaatgg actgtatcca tttggatgca ctcccaagga agtcccgtta atgaagacct
     8881 cagtctccat gtaggcacca tcaaactgca attcaacacg acctgccgca acctcttctg
     8941 atgcggtaaa tgccttgcgg taccagccag tcccgcctgg cttgtagcca ctctcagcct
     9001 cacctgattg ggtatagtcc tgaatcaaac taaagtcgtg gggcaaatcc aaaacctgcc
     9061 agcttgaatc atcaaactgc ttggcagatg cacctgcagt ctcacccaag ctaaatttcc
     9121 aggcttggtc gaaattgacc cgttggtcca cctggctata atgatggatg gtcgtccagc
     9181 tagctggcgc cacaacctgt tcttgggatt gaactgttgc ttctcgactc tcaccgccct
     9241 ctccattatt aatttgagga aggtccgttg ctggtgccaa cataggctct gcagccaccg
     9301 cctcaagttc tgctggctct gtcacagatt ctgagccatc ttgtccgacc tcttcccctt
     9361 cggatagaac tgctggtgtt tcttccacag cttcttcctc tggaatgacc gacaattctg
     9421 tctcatcggt caccaattct aaggatgggt cactcgcctc tactgtattg gcctgaacca
     9481 tttgactacc gaacaacata caacccacaa caacagaagc tgtaccaaca cttagcctcc
     9541 gaattgagta gctgtatcgc ttgtcaaata agtcttttga actacgtttc atctagtcct
     9601 cctttactat ttttcgctta tgtaagcgct tatatattgt atcaagcata ttataacgtt
     9661 ttattttatc tttttcaata tgttaaccta ttgaaaaaga actttcccca gatggtgaaa
     9721 caaaaaaact gacggacagg ctattataaa ttggactttg tctagaagac gtataaaagg
     9781 taaatcgaaa tacaaaaaaa gaacctgaaa caggttcttt ctgaattgtt gacacaaact
     9841 acgttcgctt catctccaac ctccaacagt ctatccccag actgttggag cgagttaaaa
     9901 atctggcaaa ctttagaagt ctgggaagag tcttctaaag gtcgttcctc aaaaattata
     9961 atggagcaaa caggctgctt gtgtgctaaa acacactgtg atgaattcct atttttgctg
    10021 gactttctta acgctctcac atcttgatta gtcgaagaca gcctaaaaac ttggaaaaag
    10081 agaatgaaaa aacaaaaaaa agaagctgtg acaaaagtcc tggcctctct gaattgaact
    10141 cgaacgaaaa tcctagtgaa aaagatagat tgccttgcga cgagtcgctg tgacaatctc
    10201 ctattttcat acggattttt ttgcgttctc gtatcttg
//
LOCUS       DATUCG010000090        10231 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.092, whole genome shotgun sequence.
ACCESSION   DATUCG010000090 DATUCG010000000
VERSION     DATUCG010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10231
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.092"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(20..1195)
                     /locus_tag="VCA20_001725"
                     /pseudo
     CDS             complement(20..1195)
                     /locus_tag="VCA20_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638844.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
     gene            complement(1318..1872)
                     /locus_tag="VCA20_001726"
     CDS             complement(1318..1872)
                     /locus_tag="VCA20_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009911037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEP1835507.1"
                     /translation="MNITLKKNLKTTIAGLVAGISLLTAGVVSAETYTVKSGDTLSEI
                     AETYNTTVEKLAEQNKITNLDFIHVGQVIELGDVTVKAVESSVEQTQSTSTSTATATT
                     STYSSNLSAEDAAAKEWIAMKESSGSYDARNGIYIGRYQLTNTYLNGDYSPENQERVA
                     DAYVASRYGSWSAAKAFWLANGWY"
     gene            complement(2004..2798)
                     /locus_tag="VCA20_001727"
     CDS             complement(2004..2798)
                     /locus_tag="VCA20_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEP1835508.1"
                     /translation="MDKLILGRFIPGDSIIHRLDPRSKLLAMFGFLLMIFWANNLVTN
                     ALLIAFVLGMVVLSRIRLSFFINGLKPMIGIILFTTFFQVFFTPGATILWEFWIFKLS
                     VEGLQQAAIIFVRFVLIIFFSTLLTLTTTPLSLADGIESGLAPLKKFKVPVHEIGLML
                     SMSLRFVPTLMDDTTRIMNAQRARGVDFNEGNLIQKVKSVIPILIPLFASSFKRADAL
                     ATAMEARGYQGGDGRTKYRILEWKLADTLAILVMLALAGLLFILKK"
     gene            complement(2791..3633)
                     /locus_tag="VCA20_001728"
     CDS             complement(2791..3633)
                     /locus_tag="VCA20_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEP1835509.1"
                     /translation="MGIDLQEVSYTYQAGTPFEGRALFGVDLEIVDGSYTALIGHTGS
                     GKSTILQLLNGLNVPTEGRVVIDDVVITATSENKDIKQVRKKVGLVFQFPESQVFDET
                     VLKDVAFGPQNFGVSQEEAETIAREKLALVGIDESLFERSPFELSGGQMRRVAIAGIL
                     AMEPSILVLDEPTAGLDPAGRQELMDIFKKLHESGMTIVLVTHLMDDVANYADFVYIM
                     EKGKLVRSGKPVDIFQEVDFLESIQLGVPKITKFAASLERRGFVFERLPITIEEFTGM
                     VTHG"
     gene            complement(3609..4445)
                     /locus_tag="VCA20_001729"
     CDS             complement(3609..4445)
                     /locus_tag="VCA20_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEP1835510.1"
                     /translation="MMNIIEVKNLKYKYNQEDEQYTLNDVSFHVKQGEWLSIIGHNGS
                     GKSTTVRLIDGLLEAESGDIYIDGDALTVDNVWDKRRLIGMVFQNPDNQFVGATVEDD
                     VAFGLENQGISLEKMRSRVDEALELVGMTDFKTREPARLSGGQKQRVAIAGVVALRPK
                     IIILDEATSMLDPEGRLDLIKIVREIKERHGMTVISITHDLDEVALSDRVIVMKNGQV
                     ESISTPNELFMREDLVDLDLDRPFTTELASSLRQTGLDLPLRYFTEEELEETLWELIS
                     KK"
     gene            complement(4442..4981)
                     /gene="pgsA"
                     /locus_tag="VCA20_001730"
     CDS             complement(4442..4981)
                     /gene="pgsA"
                     /locus_tag="VCA20_001730"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEP1835511.1"
                     /translation="MKKENIPNALTIGRILVIPIFILLLTVWDSTISHVLAALIFALA
                     SITDYLDGYLARKWQVVTNFGKFADPMADKILVMTAFIMLVELGFAPAWVVAIIICRE
                     LAVTGLRLLLVENGGTVLAAAMPGKIKTFSQMFAVIFLLLHWNVVGQITLYIALFFTL
                     YSGYDYFKGASFLFKDTFK"
     gene            complement(4991..5848)
                     /locus_tag="VCA20_001731"
     CDS             complement(4991..5848)
                     /locus_tag="VCA20_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEP1835512.1"
                     /translation="MRQKSIGEVLRIAREGRGWTFVDLQRITKIQAKYLQALEYNDFD
                     FIANSDDAQSILKAYADALELDADVLLDAYETNSLVKYYEDGEEELRSSELKRSYKVR
                     KRKKNSYLPLIYLLLATGLIIIFVTYIVHSRLQNRASITPTTTSYSIVEQSVSEASSG
                     TETSSTEQAQSNSTSSSSSATENVTISGSGSNIVATLKTVTYPVEVTVTAKNTTSWIS
                     LSDTLLEGGVTLTPDNPTVTTTVEEGVTSTTLVLGVVKGVEVTVGGQKLDLSALTADT
                     GSITINFEQ"
     gene            complement(5930..7213)
                     /locus_tag="VCA20_001732"
     CDS             complement(5930..7213)
                     /locus_tag="VCA20_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEP1835513.1"
                     /translation="MKLVEKKYPYMKESVFYGKVDNGLTVILLPKVDFHETYGILTTN
                     FGGIHTRFTLANGNSVVYPAGIAHFLEHKLFETENEEDIMNEFAKLGASANAYTSFRQ
                     TSHLFSTTQKVLESLSLLQSFVREPYFTEDNVEREQGIIEQEIEMYQDDADYRLFTGI
                     LSSLYPESPLAYDIAGTVESIAAITADDLHENFDVFYHPSNMNLFVIGNFDLEAVCKQ
                     ISSYQVAQMDNPAQSFELAGVQKLPIQEHLSEQFEVSTPKMAVGLRGNDEVDKETIQK
                     YRFSLQFLFAMLFGWTSKRYQQLYEQGKIDSSFQFQLEVTPEYHYLIISGDTQEPITL
                     SSILMKALRKFEDDADVTEDHLQLLKNEMYGDFIRSLNSLEFTASHFVSQYSEYENVF
                     DIPKMIQSLQLDDIREAGRRFIDHCDMTDFTIFPK"
     gene            complement(7210..8463)
                     /locus_tag="VCA20_001733"
     CDS             complement(7210..8463)
                     /locus_tag="VCA20_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEP1835514.1"
                     /translation="MKLQEGVDLHFIDTDQFTTNRIRIRFAAEMSEATVAGRVLVANI
                     FEMGNQEFQTAQAVRRRLAELYGAQFSTSVSKRGGVHCVDVTISYVSPRHLPENEDIT
                     VEILDFLYTCIFRPLKKGRGFDSQIFEVEKTNLINFLQSEIEDNFYHADVEMSKLFYK
                     DPSLQVPRVGRLDLVEKETAESTFQIYRNMLRMDKIDIFVLGKVDREQVKRKLEDFGF
                     TYRNPKLELEYHQEYSNITQEKIERKQARQSILELAYHLQVIYNDVNYPALMVFNGLL
                     GAFSHSKLFMNVREKESLAYTIGSQVSIFSGMLKVYAGISRENRLRVMKLISKQLLDL
                     KCGKFTEEELELTKNMLIHSATLAQDRQNNLIEQVYNQVTLGNRNLSWLDWIESIKSV
                     SIDDVIRVGQMINLQAVYFMEGTEE"
     gene            8537..8920
                     /gene="yaaA"
                     /locus_tag="VCA20_001734"
     CDS             8537..8920
                     /gene="yaaA"
                     /locus_tag="VCA20_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEP1835515.1"
                     /translation="MEFKLFDDYITLQALLKTTGILHSGGAIKGFLEENTILFNGEDE
                     KRRGKKIRVGDVITLPDHNTSITIVTPSAEEIKQYQEDQAEKERVAAIVKKLNQENKK
                     NKKQVKTPKKTTKNTERKPVRFPGM"
     gene            8923..10017
                     /gene="recF"
                     /locus_tag="VCA20_001735"
     CDS             8923..10017
                     /gene="recF"
                     /locus_tag="VCA20_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEP1835516.1"
                     /translation="MWLERLELQHFRNYNQLDIEFHKGLNVFLGENAQGKTNILESIY
                     VLALTRSHRTRTDKDLLQFQEKELSVSGLLHRTSGKVPLDIHLTDMGRVTKVNHLKQA
                     KLSNYIGHMNVVLFAPEDLQLIKGAPALRRKFIDVELGQIKPLYLSDLSNYNHVLKQR
                     NTYLKSTDKIDENFLSVLDQQLAEYGSRVIQHRIDFLKKLEEFGNRKVQEISGNREEL
                     TIEYQTSIKLTDDVNLIDKFLTELERCRKRDLFKKNTGVGPHRDDVAFFINGMNAHYG
                     SQGQHRSLVLSLKLAEIELMKEVTREYPILLLDDVMSELDNNRQIKLLETITDTIQTF
                     ITTTSLDHLHKLPDSLKIFHIESGKVTESE"
BASE COUNT         3314 a         2256 c         1592 g         3069 t
ORIGIN      
        1 ttttgtttca tttaactata tctgtaaaag tccaaacaac ttcttgctcc tgcatttgtc
       61 cagtaatact gattatcagg gattttacag cagcatattt tcgtgcattg tccaccaaat
      121 tatctagaat tctacgaaat tgaattggat caatctcaac tggcaatcca tccgatactt
      181 ccaaactaaa ccgaatgccc tcatctacta attcctcagt tttctctctt acataggact
      241 gtaaaaagat agccaaatca agctggactt tatcaaatgg cattttatct gtctgcaatt
      301 gtgaaaaagc aaataatttt tccagtaatt tctccatcac ctgagatttt tgatagataa
      361 cagtcaaata ctgattacgt ttttcatccg tattggcaat accatccaaa attcccttgg
      421 cataaccttt gatagaagtc aagggagtcc gcaaatcatg cgaaatatct gtgaccatct
      481 gggttcgatt ttgttcatac agttgatttt tctcacgggc atctaacaaa gaagtctgca
      541 tttgattgaa cccttgggtc aattcttcaa attccttatc accttgatag gtcagaggtg
      601 ctgtataatc tccctgctgg atacgttcta caccgctgtg caattcctcc agaggacgcg
      661 tgataacccc taggagacgg cgagtaaaga agaagttaag caccactacg accacgatac
      721 ctattccacc aactagcaaa agctgtatca ggaaggcttg aaattcttgg ctttcttgct
      781 ggtcttccaa atcttctaac aaggcatata aataataaat ttgcccatcc gtggcgagtt
      841 tacggctaat cacaatttct ccatctacat aggaggtgtg agtggttgat gatactgtca
      901 ctcccaatat ctcatccgca tcatctttca gatttgaaaa gacagcctca ccattacttt
      961 ctacatagag tcgtgcatca ttcgatgcta actcatttgc caatactgca aaatctgatc
     1021 ccgaaaactt ttccaatctc tgttgactac ttgctgtctt ctggctagtg gactgccatg
     1081 tttcttggtt acgataaata ctattaacca cataaacact tacccccagc atcgcaacca
     1141 aggaaaaaat aagcatgatc atatttaatc gaaaaagtcg tttttgtaat ttcatagttc
     1201 ttcttttcta gtcaattttt tcacaattat aaacaaaaca accctatctg taaagctaga
     1261 caactttcaa caatagaaaa aagtctagca tcgtgcttag acctcttcta atttatctta
     1321 gtaccaacca tttgccaacc agaaagcctt ggctgcagac catgaaccat agcgactagc
     1381 aacataagca tctgctacac gctcttggtt ttctggtgag taatcaccat tcaagtaagt
     1441 atttgtcaac tggtaacgac caatgtagat accgttacgt gcatcatagc taccgcttga
     1501 ttccttcatt gcaatccatt cttttgctgc tgcatcttca gcactcaagt ttgaagaata
     1561 ggtgctagtt gttgctgttg cagtagaagt tgatgttgat tgagtttgtt caactgaact
     1621 ctctactgcc ttcactgtca catcacccaa ttcgataact tgaccgacat ggataaaatc
     1681 taagttggta atcttattct gctctgctaa tttttcaaca gtagtattgt atgtttcagc
     1741 aatttcagac agggtatcac cagactttac agtataggtt tctgcactga ctacacccgc
     1801 agtcaaaagg gagattcccg ctaccaaacc agcaatagtt gtttttaagt ttttctttaa
     1861 tgtaatgttc ataatagtca cattcctttc tgtcatgtgt ctatcatacc ctctaactat
     1921 tacttatact ttgtgaaatt gttacaaata ttacagacaa attgagatta gacaataaaa
     1981 acgctatttc tagcgttttt tactcatttt ttcaatataa ataagagtcc agctaaagct
     2041 aacataacta aaattgctaa cgtatctgct aacttccatt ccaaaatccg gtacttggtt
     2101 ctcccatctc cgccttgata cccacgcgcc tccatcgccg ttgcaagagc atctgccctc
     2161 ttaaaacttg atgcgaataa cggaatcaaa ataggaataa ctgactttac cttctgaata
     2221 agatttccct cattaaagtc cactccacga gctctctgag cattcataat ccgagtggta
     2281 tcatccatca aggtcggtac aaaacgcaag ctcatggaca acatcaaacc aatttcatgc
     2341 acaggaactt taaatttctt caaaggtgca agtcctgact caataccatc agccaaactg
     2401 agcggtgttg tcgtcaaggt caatagggtt gagaagaaaa taatcaaaac aaagcgaaca
     2461 aaaataatcg ccgcctgttg caacccttct acagataatt taaaaatcca aaattcccat
     2521 agaatggtcg ctccaggtgt aaagaaaacc tggaagaaag ttgtaaatag aataattcct
     2581 atcatcggtt tcaaaccatt aataaaaaac gataagcgaa tacgagataa aacaaccatt
     2641 cctaaaacaa atgctatcaa caaggcattg gtgaccaaat tattggccca aaaaatcatc
     2701 aatagaaatc caaacattgc taaaagctta cttcttggat ccaaacgatg aataattgaa
     2761 tccccaggaa taaagcgacc taaaattaat ttatccatgt gtcaccatcc ccgtaaattc
     2821 ttcaattgta ataggaagac gttcaaatac aaaacctctt ctctccaaac tagctgcaaa
     2881 cttagtaatc ttaggcactc ccaactgaat actctctaaa aaatctacct cttgaaaaat
     2941 atcgactggt ttaccagaac ggaccagctt tcccttttcc ataatgtaaa caaaatcagc
     3001 atagttagcg acatcatcca tcaaatgagt taccaaaacg attgtcatgc ctgattcatg
     3061 aagctttttg aaaatatcca tcaattcctg acgacccgca ggatccaatc cggccgttgg
     3121 ttcatccaaa actaagatac ttggttccat tgccagaatc ccagcaatag ctacccgacg
     3181 catttgacca ccagatagtt caaaaggact acgctcaaac aagctttcat caattccaac
     3241 caaggccaac ttttcacgcg caatcgtttc agcctcttcc tgagaaactc cgaaattttg
     3301 tggaccaaaa gcaacatctt tcaaaactgt ctcatcaaac acttgacttt caggaaattg
     3361 gaacaccaac cccactttct tcctgacttg cttaatatcc ttattttcag acgtcgctgt
     3421 aatcacaaca tcatcaatga ctactcttcc ttctgttggt acatttaatc cattcaagag
     3481 ttgtaaaatc gttgatttac ccgaacccgt atgcccaata agggccgtgt aagagccatc
     3541 aacaatctcc aaatccacac caaaaagcgc acgcccctca aaaggagtgc cagcttgata
     3601 ggtatagctt acttcttgga gatcaattcc cataaagttt cctctaattc ttcctctgta
     3661 aaatagcgga gcggtaaatc aagtccagtc tggcgcaaag atgaagccag ttctgtcgta
     3721 aacggtctat ccaagtctaa atctaccaaa tcttctcgca taaatagttc atttggagtg
     3781 ctaattgatt caacctgtcc atttttcatc acgataactc ggtcactcaa tgcaacctca
     3841 tccaaatcat gtgttataga gataaccgtc ataccatgac gctccttaat ttcacggaca
     3901 atcttaatca agtcgagacg accttctgga tccaacatag acgtagcctc atccaaaata
     3961 ataatttttg gacgcaaggc aaccactcca gcaatcgcca ccctctgctt ctgaccacca
     4021 gataaacgag caggctcacg tgttttgaaa tcagtcattc caaccaattc caaagcttcg
     4081 tcaactcgac tacgcatctt ctccaaagag attccctggt tctctaaacc aaatgcaaca
     4141 tcatcttcaa ctgtagcacc cacaaattgg ttatctggat tttgaaaaac cataccaatc
     4201 aaacgccgtt tatcccagac attatcaacg gttaaggcat caccgtcaat ataaatatca
     4261 cctgattcag cttccaacaa accatcaatc aatcgaacag ttgtcgattt cccagaacca
     4321 ttatgaccaa taatagacag ccactctccc tgtttcacgt gaaacgaaac atcatttaga
     4381 gtatattgct catcctcttg gttgtattta tattttaaat tttttacttc aataatattc
     4441 atcatttaaa tgtatcttta aacaagaagg acgcaccctt gaaataatca taacctgaat
     4501 atagggtgaa gaaaagtgca atatagagtg ttatttgccc cacgacgttc cagtgtaaga
     4561 gcaggaaaat aactgcaaac atctgtgaaa atgttttgat ttttcctggc atagccgcag
     4621 ctagaactgt tcctccattt tccaccaaga gtagacgaag acctgtcact gccaattcac
     4681 gacaaataat aattgcaact acccaagctg gagcaaagcc aagctctaca agcatgataa
     4741 atgctgtcat caccaaaatc ttatctgcca tcggatccgc aaattttcca aagtttgtta
     4801 caacctgcca tttacgagcc aagtagccgt ctagataatc cgtaatacta gctaatgcaa
     4861 aaataagtgc tgctaagaca tgactaattg ttgaatccca aacggtcagc aacaagataa
     4921 aaattggaat taccaaaatc ctacctatcg tcaatgcatt gggaatattt tcttttttca
     4981 tataaaacct ttattgttca aaatttattg taattgagcc tgtatctgct gtcaaggctg
     5041 atagatctaa cttttggcca cctacagtta cctctactcc tttgacaaca ccaagaacaa
     5101 gtgtagtaga agtcactcct tcttcaacag tagtagttac tgttggatta tcaggtgtta
     5161 gtgtaacgcc cccctctaac aaggtgtcag aaaggctgat ccaactcgtt gtattttttg
     5221 ctgttacagt tacttctact ggataagtca ctgttttaag ggtcgcaaca atgttagaac
     5281 ctgatcctga aatagtcaca ttttctgtag cggaactact tgaactcgtt gaatttgact
     5341 gcgcctgctc agtagaactc gtctctgtgc cactacttgc ttcagataca gattgctcaa
     5401 ctatactata tgacgttgta gtaggcgtaa tagatgctct attttggagc ctgctatgaa
     5461 caatataagt cacaaaaata ataatcaaac ctgtcgcaag taataagtaa atcaacggaa
     5521 gataagagtt ctttttacgt ttacgaacct tatagcttct tttcaattct gaagagcgta
     5581 gttcttcttc tccatcttca taatatttca ctaaactatt ggtctcgtaa gcatccagta
     5641 gaacatctgc atctaattcc aaagcatcag catatgcttt taaaatagac tgagcatcat
     5701 ctgagtttgc aataaaatca aaatcattgt actcaagtgc ctgcaaatat ttagcttgga
     5761 tttttgttat ccgttgcaag tcaacaaatg tccaaccccg tccttctcta gctatcctaa
     5821 gtacttcccc tatactcttt tgtctcataa taccctctct ctatcgctca tcatgtacag
     5881 tatactattc taacaaaatt ccaaggaaat ttcgacgggt tgcgcccact tattttggaa
     5941 aaatcgtgaa atctgtcata tcacagtgat caataaagcg acgacctgcc tctctgatgt
     6001 catctaactg aagactttgt atcattttag gtatatcaaa aacattttca tattctgaat
     6061 actgagaaac aaagtgacta gctgtaaact caagagaatt taagctacga ataaaatctc
     6121 catacatctc gtttttcaac aattgcaaat gatcctctgt cacatctgca tcatcctcaa
     6181 atttcctaag tgctttcatc agtatactag aaagcgtaat gggttcttga gtatctcccg
     6241 agataatcaa atagtggtat tcaggtgtaa cctctaattg gaactggaaa gaagaatcaa
     6301 tttttccctg ctcgtataac tgttgatatc gttttgaagt ccaaccaaac aacatggcaa
     6361 ataaaaattg taaactaaac cgatatttct gaatagtctc cttatcaacc tcgtcatttc
     6421 cccgtaaacc aacagccatt tttggggtag atacttcaaa ttgttctgag agatgttcct
     6481 gtatcggcaa cttttggaca cctgccaatt caaacgactg agctggatta tccatctgag
     6541 ctacttgata acttgaaatt tgtttacaaa ctgcctctaa atcaaaatta ccaataacaa
     6601 ataaattcat gttacttgga tgataaaaca catcaaaatt ctcatgtaaa tcatccgcag
     6661 taattgcagc aattgattca actgtccctg ctatatcata ggccaaagga ctttctggat
     6721 aaagcgatga taaaattccc gtaaacaagc gataatctgc atcatcttga tacatctcaa
     6781 tttcttgttc aataatcccc tgttctcttt ctacattgtc ttctgtaaaa tacggttctc
     6841 gaacaaaaga ttgtaaaagt gatagagact ccaatacttt ctgagtcgtt gaaaataagt
     6901 gactagtctg tctaaaactg gtataagcat ttgcgcttgc tcccaattta gcaaactcat
     6961 tcataatatc ctcttcattc tcagtttcaa atagcttatg ttctaaaaaa tgtgcaatcc
     7021 ctgcaggata aacaacacta ttaccattag ccaatgtaaa tcgagtatga atacccccaa
     7081 aatttgtcgt tagaattcca tatgtctcat ggaaatctac tttaggaagc aaaataactg
     7141 tcaacccatt gtcaactttc ccataaaaga cagattcttt catgtaagga tacttcttct
     7201 caactaactt cattcttctg tcccctccat aaagtaaaca gcctgtaaat taatcatctg
     7261 tcctactcga atgacatcgt ctattgatac cgatttgata gactcaatcc aatctaacca
     7321 acttaaattt ctatttccta aggtaacttg attatatact tgttctatca aattattctg
     7381 cctatcttga gccaaggttg ctgaatgaat caacatgttt tttgtcaact ctaattcttc
     7441 ttctgtaaac ttaccacatt ttaaatcaag tagttgttta ctaattaact tcattaccct
     7501 gagtctattt tcacggctaa ttccagcata gacctttagc attcctgaaa aaatagagac
     7561 ctgactgcca attgtatagg ccaaactttc tttctcacga acattcataa ataacttcga
     7621 atgggagaaa gcacctagta gaccattaaa taccatcaaa gctggatagt ttacatcgtt
     7681 gtaaataact tgtaaatgat atgccaattc caaaatggac tgccttgcct gtttacgctc
     7741 gattttttct tgcgtgatat ttgagtattc ctgatgatat tctaactcta attttggatt
     7801 tctataagta aaaccaaaat cttcaagttt tcttttgact tgttctctgt caaccttccc
     7861 taagacaaat atatcaattt tatccatacg caacatattc cgataaatct gaaaggttga
     7921 ttctgctgtt tctttttcaa ccaaatcaag cctaccgacg cgtggaactt gaagagaggg
     7981 atctttataa aaaagcttac tcatttcaac atctgcatga taaaaattat cttctatctc
     8041 tgactgaaga aaattgatta aattcgtttt ttcaacctcg aaaatctggc tatcaaatcc
     8101 tcgccccttt ttcagtggtc taaatataca tgtgtataaa aaatcaagaa tctctactgt
     8161 aatatcctca ttttctggca agtgacgagg actgacatat gaaattgtca catctacaca
     8221 gtgcacccca ccacgtttcg aaactgaggt cgagaactga gcaccataca attctgccaa
     8281 tcttctccga acagcctgag cagtctgaaa ttcttggtta cccatttcaa aaatatttgc
     8341 aactaacaca cgaccagcaa ctgtagcctc actcatttca gctgcaaagc gaatacgtat
     8401 acgatttgtc gtaaactgat ctgtatcaat aaaatgaaga tctactccct cttgtaattt
     8461 catcgtcttc ctctttcaaa cattgtacct tccattatac cattttgtga tataatattc
     8521 tgtaacgagg tgacttatgg aatttaaatt atttgatgac tacatcacat tacaagcatt
     8581 gttaaaaacg actggtattc tccattcagg aggtgccatt aaaggtttct tagaagaaaa
     8641 taccattctt ttcaatggag aggatgaaaa aagacgcggt aaaaaaatcc gtgtcggaga
     8701 tgttatcaca ctcccagacc acaacaccag catcacaatc gtcactccta gtgcagagga
     8761 aatcaaacag taccaagagg atcaagctga aaaagaacgt gtggctgcaa tcgttaaaaa
     8821 gttaaatcaa gaaaataaaa agaataagaa acaggtaaaa actcctaaaa aaacaactaa
     8881 aaatactgaa agaaaacctg ttcgtttccc tggtatgtag acatgtggct agaacgatta
     8941 gagttacaac attttcgcaa ttacaaccaa ttagatattg agttccacaa agggctcaat
     9001 gtctttttag gcgaaaacgc ccaaggaaaa accaatattc tagaatctat ctatgtttta
     9061 gccttaacac gaagtcatcg aacacggaca gataaggacc tattacagtt tcaagaaaag
     9121 gaattatctg tttctggttt actccatcgc acaagcggta aagttccact tgacattcac
     9181 ttgacagaca tggggcgagt gaccaaggtc aaccatctca aacaagcaaa actttctaat
     9241 tacattggac atatgaacgt cgtcctattt gctccagaag atttacagct gataaaaggt
     9301 gcgcctgctt tgagaagaaa gttcattgat gtcgagctcg ggcaaatcaa gccactctat
     9361 ctctctgacc tatcgaatta taaccacgtt ttaaaacaaa gaaatactta cctcaaatct
     9421 acagataaga ttgatgagaa cttcctatcc gtgttagatc agcaacttgc tgaatatggt
     9481 agtcgtgtta tccaacatcg tattgacttt ctaaagaaat tagaggaatt cggaaataga
     9541 aaagttcaag aaatatctgg caatcgggaa gaattgacaa tcgaatacca aacctcaatc
     9601 aaattgacag atgatgtcaa tttaattgac aaattcttga cggaattgga aaggtgtcgc
     9661 aaacgtgatt tatttaagaa aaatacagga gttggaccac atcgagatga tgtagctttc
     9721 tttataaatg gaatgaatgc ccattatggt agtcaaggtc agcaccgcag tctcgttctg
     9781 tcactcaaac tggctgaaat tgagctgatg aaggaggtca cacgagaata cccaattcta
     9841 ctcttagatg atgttatgag tgagctggat aataatcgtc aaatcaaact actagaaact
     9901 attacagata cgattcaaac atttattaca acgacatctt tagaccattt acataagcta
     9961 cctgatagtt taaaaatctt ccatattgag tctggtaagg ttactgagtc agaataagat
    10021 tgtttacact gtcgtcaata aagtttacaa aaaagtaaag cccctgtcaa cacaatgtca
    10081 ttaagttaag catttaagca gctaaagaaa ctattcccct ctgagcaatt gatgacaaaa
    10141 cgggaatagt ttttgtattt agggcgcaca aaaaggaggt tttttaaccc tatttttcaa
    10201 ctattatgtt actctataag tgaagcttac a
//
LOCUS       DATUCG010000091        10181 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.093, whole genome shotgun sequence.
ACCESSION   DATUCG010000091 DATUCG010000000
VERSION     DATUCG010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10181
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.093"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            164..547
                     /locus_tag="VCA20_001736"
     CDS             164..547
                     /locus_tag="VCA20_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835517.1"
                     /translation="MKELEKIMEQIEKEADKIEKAQNRKKQLNQKMSKLKRSAETQRK
                     IRKGGVFEAFEREITGRQEDTNNDLIYAFLDYVLSDNRYREKLKELTAIHLKDRETNM
                     GETKNSDDENDSIDSQKVENSDEDM"
     gene            1001..2701
                     /locus_tag="VCA20_001737"
     CDS             1001..2701
                     /locus_tag="VCA20_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HEP1835518.1"
                     /translation="MEIKSLHTHVDIVSRSKGASVIAKAAYNARDKLRDEYYVKVHDY
                     SKKDDLVFSKIFLPEHIPKEFSNREFLWNSVEKIEKSKNSQLARNLLFTLPRELSEED
                     RIKLISEFIEENFTSKGMIADCNIHNPTASDNEEQPHAHILLTLREIDEKGNWKPKCR
                     KEYILDENGEKIKLKSGNYKSRKVNLNDWNELDKAKEWRENFSKKANEYLAKNNIDKR
                     IDPRTFEEQGREELPQIHLGTASYQMEKKGIQTERGNHNRKIIALNTEFKKLKEEIAE
                     IGSWILSLINMVKALLKGFSKEKQEEYNLTPDLFNVYSYLETYYKIQKELSKNLSYDS
                     RMRKEHFDSKKYVKALSYMSSNNLKTIIDIQCKKDEVLTKLKENKESIADCNKQIKNI
                     DTLIKQAKIMRENKTVYDRYKGADNSIFSKIAGASKEEYYNSHKNEIDKYIRAKSILK
                     KLSGIEKIETKKWEKEKRELQTDINHLTFNQKFIKEEITQINHIKYVVGEVNKDFGID
                     INIEIEIAYKKAIARGEKPSVKMALEEFQRQIKKEDRQKAWAKEHYKKKDHIHKETER
                     "
     gene            2847..3851
                     /locus_tag="VCA20_001738"
     CDS             2847..3851
                     /locus_tag="VCA20_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HEP1835519.1"
                     /translation="MAEQNKEIVVINEDTIKNRIYYIRNQKVMLDFELAEIYGYSTKR
                     FNEQVKRNNEKFDDDFMFQLTDEEVSELSRSQNATLNRGEGRGSNIKYNPYAFTEQGI
                     YMLMTVLRGELAVRQSKALIRMFKQMKDFIIENQDFIGSKELVQIAIQTNRNTNDIAE
                     IKSQMATKEDLKKVMDNFIDPETYKHFLLMNGDKIEADVAHTKIYKSAKKSIYVIDNY
                     IGLKTLELLRAARDKTQIIVFSDNVKNKDMLTKNILDDFRKDYPNIDLKLKIAGKKYH
                     DRYIAIDYGTENEAFYLCGASSKDAGNKVSSITKIEESSKDMYHDMFSKMLNNKDLKI
                     "
     gene            3939..4658
                     /locus_tag="VCA20_001739"
     CDS             3939..4658
                     /locus_tag="VCA20_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replisome organizer"
                     /protein_id="HEP1835520.1"
                     /translation="MADKRMFSLKIVDSDLFLDMPLSSQCLYFHLSMRADDDGFVDNP
                     KKIIKIIGANDDDLKILITKGFVIVFERGIIVITHWKINNFIRKDRYKPTMYIEQKQQ
                     LYQTENGTYISEENVGCHLVNQRLSSGQPSIDKGRLDKVSIDKGRIEQVAPILYGEYK
                     NIRLTDEEYQKLKNKLQNHTDTMIEKLLRYLKSKGTDYKDHYVTILNWYEQDKEKLTQ
                     KNIQNGSYKTYSTNYEDSDSL"
     gene            4785..5633
                     /locus_tag="VCA20_001740"
     CDS             4785..5633
                     /locus_tag="VCA20_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEP1835521.1"
                     /translation="MMNDKENMITTKIQGTDFTYNKVTHYEKDGHIYCKTCNERIDGK
                     AIPMLDKPMIIRTACKCDRDRQEQEKQREKRIEQDRLRQNCFISKNQKAYTFENADED
                     TDKEIIKKAKNYVKHFEEMRKDNVGLLLYGNVGSGKTYIACAISNAIITEYSYTVKMR
                     NFAQILNDLQKGGFNLDRNEYIEQITNPTLLILDDFGIERNTEYALEQIYNVINARYL
                     KARPTIITTNLNFKDIEQEQEDIMLGRIYSRIIEMCLPLRVIGVDRRKIQSKEKLKKA
                     QNLIDE"
     gene            5694..5855
                     /locus_tag="VCA20_001741"
     CDS             5694..5855
                     /locus_tag="VCA20_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019213949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecR"
                     /protein_id="HEP1835522.1"
                     /translation="MNNEKKKKNKKIKFEIKRVYVGEKPIGEVFEEVIEHIAMKNLEN
                     TKEEKQTQA"
     gene            5934..7553
                     /locus_tag="VCA20_001742"
     CDS             5934..7553
                     /locus_tag="VCA20_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEP1835523.1"
                     /translation="MNRTKKYIAALYCRLSKDDGSTNESMSIYSQKAMLKQYAEQNNI
                     AIYDYYVDDGYSGTNFERPAFKKMITDIENGKINCVITKDLSRLGRNYLESGAYIEMY
                     FPQKNVRYIAITDGIDTINSYEFDIMPFKNILNEMYAKDTSKKVKSALKSRMKEGTYI
                     GSKAPFGFKKDPDDKHRLIIDERVKPIIELVYELCLEGKGTQLISQEMMKRKIPRPSS
                     FLENADKLYGLTEENKYKWTHRMILSILRDPVYCGNMERNKRPTLSFKNRKRLYVPKE
                     DRIVVKDTHEGIVSEEVWTQVQQMLDKRKNTNKSGITYDNIFKGLVKCPDCNYALTPK
                     KDYRLNKKDTIDFVHFSCSGYKKYGVKACTSHRINARDLYDVVLEDIQYHGQMALSSR
                     EDFVMKIAEKIDKDKVDERKDKTEKLNSSKIKLKDLDKAFEKLYEDRLSESISERNFN
                     LMNEKLSRQQEKLIEEIDLLEEEIKEIADTEENCEQFVENISKFAKIKELNRYILNQV
                     IDKIYVYDKEEVDGEIKQKVEIHYKFIGKLD"
     gene            8171..9982
                     /gene="ltrA"
                     /locus_tag="VCA20_001743"
     CDS             8171..9982
                     /gene="ltrA"
                     /locus_tag="VCA20_001743"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006740542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEP1835524.1"
                     /translation="MRNPQNVLNNLTKHSKDKNYQFKRLYRLLYNEEMYLVAYQTIYA
                     NPGHMTPGVDELTIDGMSIARIDQLIDSLKDESYQPHPSRRTYIPKKNGKLRPLGIPT
                     FDDKLLQQVIKMILEAIYEGQFESSSHGFRPNKSCHTALTQIQKTYTGTKWFIEGDIK
                     SFFDNINHDVMIQILRERITDERFLRLIRKFLNAGYVEDWKFYKTYSGTPQGGIISPI
                     LANIYLDKFDKYMTDYVKNFCEGKYRKRIPEYRQNEIALGKARRTLECASTENQRQEA
                     IQRIRQLEKERVLIPHSDPMDSSFKRLTYTRYADDFICGVIGSKEDARRIKADIKDYL
                     KTVLKLELSEEKTLITNARDKARFLGYHLYIRQSNLAKRDSVGRLVRNYTGRLVLEVS
                     IETIRDRLLSYGAMKMTYHRGHEVWKPTARYFMKDCDDLEILERYNAEIRGFYNYYCI
                     ANNSSILHRFKYIMEYSMYKTYATKYRTTKSHIIRKYKKDGHFRIQYIGRKGDTMTRY
                     FYNGGFRRQKKSFLENDNLPNTAKYFSRTNLIDRLKANRCEYCQATNSLIEIHHVRKL
                     KDLKKKTFWERLMISRQRKTIALCKDCHKKLHYGKLD"
BASE COUNT         4205 a         1364 c         1979 g         2633 t
ORIGIN      
        1 acttttattg attaaaaaat ataaagttga agagtaatta aatactataa tcaaagtttt
       61 ttatcactac tttggctaca aattatagaa agaaaatagg ctacttatct attaaagata
      121 tttagccttt tgtatttata aaatttgaaa ggagaattta aagatgaaag aattagaaaa
      181 aataatggaa caaattgaga aagaagcaga caaaatagaa aaggcacaaa acagaaaaaa
      241 gcaacttaat caaaaaatga gtaaactcaa aagaagtgca gaaacacaaa gaaaaataag
      301 gaaaggtgga gtgtttgaag cctttgaaag agaaattaca ggtaggcagg aagatacaaa
      361 taacgattta atctatgcct ttttagacta tgtgctatca gataatagat atagagaaaa
      421 gctaaaggaa cttacagcaa ttcatttgaa agatagagaa acgaatatgg gagaaactaa
      481 aaattctgat gatgaaaatg acagcataga cagtcagaaa gtagaaaact cagatgaaga
      541 tatgtaagct gactgaacga aacgttctgt taggtagtaa agaataaaaa ataaagatta
      601 gataggtggc tcttatgaaa attaaatatg aaaattgatg gtgcaaagtg gaaagaaatt
      661 ttaacacttt gcttttttat tgattgttaa tcaagaaaaa acaaagtcca caggggttta
      721 agggggagta gcccccttga acaaataaaa atgctttagc gtttttgttt ccttagcgga
      781 gcgtatagct ttccgcagga acgcaaagca ccgtagtcaa acggtgtatc tttgcgccct
      841 ttgaaaaagg gagcgaagat acctaacatc ataaagatac taaaatataa aaattaaata
      901 tcggatagag attagcagat agaaaaattt taagaaaaat ctatctgctt ttcttatgcc
      961 taaaaatata aaaatcataa atggaaagga gctaaacaaa ttggagataa aaagtttgca
     1021 tacccatgta gacatagttt caaggtcaaa aggggcaagc gtaattgcaa aggctgcata
     1081 taatgcaaga gataaattaa gagatgaata ctatgtaaaa gtccatgatt attctaaaaa
     1141 agatgacctt gtcttttcaa aaatattctt gccggaacat atcccaaaag agttttctaa
     1201 cagagaattc ctatggaaca gcgttgagaa aatagagaaa agtaaaaact cacagcttgc
     1261 aagaaattta ctctttacac ttccaagaga attaagtgag gaggatagga taaaacttat
     1321 cagtgaattt attgaagaaa actttacctc taaaggtatg atagcggatt gtaatattca
     1381 taatcctaca gccagtgata atgaagaaca accacatgcc catatcctac ttacccttag
     1441 agaaattgat gaaaaaggaa attggaaacc caaatgcaga aaagaatata tccttgatga
     1501 aaatggagaa aagataaaac tgaaaagcgg taactataaa tcaagaaaag taaacttaaa
     1561 tgattggaac gaacttgaca aagcaaaaga atggagagag aatttttcaa agaaagcaaa
     1621 tgagtattta gcaaaaaata acatagataa aagaatagat cctcgtacct ttgaagaaca
     1681 aggcagagaa gaactaccac aaattcattt aggaacagca agctatcaga tggagaaaaa
     1741 aggcatacag acagaaagag gaaatcataa ccgaaaaatc atagccttaa atactgagtt
     1801 caaaaaatta aaagaagaaa ttgcagaaat aggcagttgg attttaagtt tgataaatat
     1861 ggtaaaggca ctgctgaaag gattttccaa agaaaaacag gaagaatata atctaactcc
     1921 agacctcttt aatgtctatt cctatcttga aacctactat aaaattcaaa aggaactatc
     1981 taaaaattta agttatgaca gtagaatgag aaaagaacat tttgactcta aaaagtatgt
     2041 caaagcccta tcctatatga gcagtaataa tctaaagaca atcatagata tacagtgtaa
     2101 aaaagatgaa gtattgacaa agctaaaaga aaataaggaa agcatagcgg actgtaacaa
     2161 gcaaattaaa aacatagaca ccttaatcaa acaggcaaaa ataatgagag aaaataaaac
     2221 cgtctatgat agatataagg gagcagataa ctctatcttc agcaaaatag caggagcaag
     2281 taaagaagaa tattacaatt cccataaaaa tgaaattgat aaatatatta gagcaaagtc
     2341 tatccttaaa aaattaagcg gaattgaaaa gatagaaaca aaaaaatggg agaaagaaaa
     2401 aagagagttg cagacagata taaatcatct tacttttaac caaaaattta taaaagagga
     2461 aattactcaa ataaaccata tcaaatatgt agtaggtgaa gtaaataaag actttggaat
     2521 agatataaac attgagatag aaatagccta taaaaaagct attgcaagag gagaaaagcc
     2581 aagtgtaaaa atggcacttg aggaatttca aaggcagatt aaaaaagagg ataggcaaaa
     2641 agcatgggca aaagagcatt ataaaaagaa agaccatatc cacaaggaaa ctgagagata
     2701 gaaaagaagt gatatttttg gtataattat ataaattaaa ctgaagatgt aatgatggag
     2761 aattagctta caagttgtaa gcaaaatgag ataaagagaa aatggctaca agttgtagcc
     2821 ccaaaaatat attggaggtg tattttgtgg cagagcaaaa taaagaaata gtagtaatca
     2881 atgaagatac gattaagaat aggatttact atatacgaaa tcaaaaggtt atgcttgatt
     2941 ttgaacttgc tgaaatctat ggatattcta caaaaagatt taatgaacaa gttaaaagaa
     3001 ataatgaaaa atttgatgat gattttatgt ttcaattaac tgatgaagaa gtatctgaac
     3061 tttcaaggtc gcaaaatgcg accttgaaca gaggagaagg tagaggaagc aatataaagt
     3121 acaatccata tgcctttacg gagcaaggga tttatatgct tatgactgta ttaaggggag
     3181 agcttgcagt tagacagagc aaggctttaa tacgaatgtt taagcagatg aaagacttta
     3241 ttattgaaaa tcaggatttt atcggctcaa aggaattagt acaaattgct attcaaacca
     3301 accgaaacac aaatgatatt gcagaaataa aatctcaaat ggctactaaa gaagatttga
     3361 aaaaagtaat ggataatttt atagacccgg aaacatataa acatttctta cttatgaatg
     3421 gagataagat agaagctgat gttgcccata cgaaaatata taaatcagca aagaaaagta
     3481 tttatgttat agataactat atagggctta aaacattgga acttttaagg gctgcaagag
     3541 ataaaactca aattatagtt tttagtgata atgttaaaaa taaggatatg cttacaaaaa
     3601 atatcttaga tgattttaga aaagactatc ctaatataga cctgaaactg aagatagcgg
     3661 gtaaaaaata ccacgataga tatattgcca tagactatgg aacggaaaat gaagcctttt
     3721 atctttgcgg agcttcatca aaggatgccg gaaataaggt atcaagcatt accaagatag
     3781 aagaatcttc taaagatatg taccatgata tgtttagcaa gatgttgaat aataaagact
     3841 taaaaattta atatataatt atgaaaaaat tatagcaata tgcaaggcgg tagaagtaga
     3901 aatctatcgt cttttttatt gaagaaaggt aggaataaat ggcagataaa agaatgtttt
     3961 cactaaagat agtggatagc gacttatttt tggatatgcc actaagtagt caatgcctat
     4021 attttcattt atcaatgaga gcggatgatg acggctttgt ggataatcca aagaagataa
     4081 taaaaatcat aggggcaaat gatgatgact taaaaatcct aattacgaaa ggttttgtaa
     4141 ttgtctttga aagaggaatt atagtcatta ctcattggaa gataaataat tttattagaa
     4201 aagacagata taagccaact atgtatatag agcaaaaaca gcagctttac caaacggaaa
     4261 acggaacata tatttcagaa gaaaatgttg gttgtcatct ggttaaccaa aggttgtcaa
     4321 gtggtcaacc cagtatagat aagggtagat tagataaggt tagtatagat aaggggagaa
     4381 tagagcaggt tgccccaatt ctttatggag aatataaaaa tatccgctta actgatgaag
     4441 aataccagaa gttaaagaat aagctgcaaa atcataccga tacaatgatt gaaaagctat
     4501 taaggtatct taaaagcaaa ggtacagact ataaagacca ctatgtaaca atccttaact
     4561 ggtatgaaca ggacaaagaa aaactaacac agaaaaatat tcaaaatgga agttataaaa
     4621 cctattcaac caactatgag gatagcgaca gcctataact atcaaatgag acggtggaaa
     4681 ggtgttttta agggtttaat cataaaagag gtatcagact aaggctaaga aaaaaataag
     4741 cattaaaacg cattttacga cctgatatgg aaaaggagat taagattatg aatgataaag
     4801 aaaatatgat tactacaaaa attcaaggta cggattttac ctacaataaa gttactcact
     4861 atgaaaaaga cggacatatc tattgcaaga cttgtaatga aaggatagac ggcaaagcaa
     4921 ttccaatgtt agacaagcct atgattatta gaacagcttg taaatgtgat agagacagac
     4981 aggagcaaga aaaacaaaga gaaaagcgga tagaacagga taggttaaga caaaactgct
     5041 ttatatccaa aaatcaaaaa gcatatacct ttgaaaatgc tgatgaagat accgataaag
     5101 aaatcatcaa aaaagctaaa aactatgtaa agcattttga agaaatgaga aaggacaatg
     5161 taggcttgct tttatatgga aatgtaggaa gcggtaagac ctatatagct tgtgctattt
     5221 ccaatgccat aatcacagag tattcttata cagttaagat gagaaatttc gcacagatac
     5281 taaatgactt acaaaaaggt ggatttaacc ttgatagaaa cgaatatata gagcaaataa
     5341 caaatcctac tctactaatc cttgatgatt ttggaataga aaggaataca gaatacgcct
     5401 tagagcaaat ctacaatgta atcaatgcaa gatacttaaa ggcaagaccg accattataa
     5461 ctaccaacct gaactttaag gatatagaac aagaacagga agatataatg cttggaagaa
     5521 tttattcaag gataatagag atgtgtttac cccttagagt tataggagta gatagaagaa
     5581 agatacaaag caaagaaaaa ctgaagaaag cacaaaactt aatagatgag taaaggcggt
     5641 tagagagtaa aaaatctaat cgctttttta ttacaaggaa agggcggatg tagatgaata
     5701 atgaaaaaaa gaagaaaaac aagaaaataa aatttgaaat taaaagggtt tatgtaggtg
     5761 aaaaacctat tggagaagtg tttgaggaag taattgaaca tatagctatg aaaaatttag
     5821 aaaatacaaa agaagaaaag caaacgcaag cgtgacgaat gattgaatat acaggagtat
     5881 tttggtataa taatcatatt gagatagtcc tgttaaaaga aaggagcgta aaattgaaca
     5941 ggactaaaaa atatatcgca gctttgtatt gtagattgtc aaaagacgac ggtagtacaa
     6001 atgagagcat gagtatatac tcacaaaaag caatgctaaa acagtatgcg gaacaaaaca
     6061 acatagctat atatgattat tatgttgatg atggctattc agggacaaac tttgaaagac
     6121 cggcattcaa aaagatgata accgatatag aaaacggaaa aataaattgt gttatcacaa
     6181 aagatttatc aagacttgga agaaactatc ttgaaagtgg tgcatatatt gagatgtatt
     6241 ttccacaaaa aaatgtaagg tatatcgcaa tcactgacgg aatagacacc ataaattcct
     6301 atgagtttga tataatgcca tttaagaata tcttaaatga aatgtatgcc aaagatactt
     6361 ctaagaaagt aaaaagtgcc ttgaaaagta gaatgaaaga gggaacatat ataggttcaa
     6421 aagctccatt tggctttaag aaagaccctg atgacaagca taggttaatc atagatgaaa
     6481 gagtaaaacc tattatagag cttgtctatg agctttgttt agagggaaaa ggaacacagc
     6541 ttataagtca ggaaatgatg aaaaggaaaa taccaagacc aagttccttt ttagaaaatg
     6601 cagataagct gtatggactg acagaagaaa ataagtacaa atggacacat agaatgattc
     6661 ttagtatctt aagagaccct gtctattgtg gaaatatgga aagaaacaaa agacctactc
     6721 tttcattcaa gaatagaaaa aggctatatg ttcccaaaga agatagaatt gttgtaaaag
     6781 atactcatga gggcattgta agtgaggagg tatggacaca agtacaacaa atgcttgata
     6841 aaagaaagaa tacaaataaa agtggtataa cctatgataa tatcttcaaa ggtttagtta
     6901 aatgccctga ttgtaattat gctttgacac caaagaaaga ttatagactg aataaaaaag
     6961 atacaataga ttttgtgcat ttctcatgtt caggttataa aaagtatgga gtaaaggctt
     7021 gtacatcaca taggataaat gcaagagatt tatacgatgt tgtacttgaa gatatacagt
     7081 atcatgggca aatggcacta tcaagcagag aagattttgt aatgaaaatt gctgagaaga
     7141 tagataaaga taaggttgat gaaagaaagg ataaaactga aaagctaaat agtagtaaaa
     7201 ttaaacttaa agacttagat aaagcctttg aaaaacttta tgaagataga ctgagtgaaa
     7261 gtatatcaga gcgtaatttt aaccttatga atgaaaaact atcaaggcag caagagaaac
     7321 taattgaaga aatagacttg cttgaagaag aaataaagga aatagcggat acagaagaaa
     7381 actgtgagca gtttgtagag aatataagca agtttgcaaa gataaaagag ttaaaccgtt
     7441 acattcttaa tcaagttata gacaagatat atgtatatga caaggaagaa gttgacggag
     7501 aaataaagca gaaagtagaa atccactata aatttatagg aaaattagat tgaccactta
     7561 ggacagttca actgacattg tccgtgcgtc cgtaaaggcg gtatagtaaa tctacttttt
     7621 ggcaaagtag taggcgtgac cttaatgcct atcatcaccc aactgacctg tgagggaaac
     7681 caaatatcag ggaacaaagc acgttgggaa actcataagt tgatgagtta tcacatcacg
     7741 actgaatgag gagatgacaa tgtaacagtt gttactgaga taattataag gataaaatct
     7801 aaactgattg aaggatagtc taaacggtct gtgtcgggac tatgaggtaa gataactaaa
     7861 accctcatac tatggaactt cactatcact ggataagata atgaagcaaa gggattccat
     7921 agcgtattca tagtaaatga aaacagatga acgtcctatc cgaagaaatt atcaagctat
     7981 gttactatac cagctaaacg gagattacct aagtcaaaac gcttgtaaaa gctattaaac
     8041 atcaggttta tgaatatttg ataaggtaac ggagttccca tagtagtccg agcgtgttaa
     8101 tggcacgtac atggcgaagg gaaacagttt ataacattaa cataatggaa aggaaggtgt
     8161 gtgagacacc atgagaaatc ctcaaaatgt gttaaacaat ctaacaaaac acagtaaaga
     8221 taaaaactat caatttaagc gactttatcg tttgctttat aacgaagaga tgtatttagt
     8281 tgcctatcaa acgatttacg ctaacccagg acacatgact cctggagtag acgagttaac
     8341 aattgatggc atgagtatcg ctagaattga ccaattgatt gattccctga aagatgaatc
     8401 ctatcagcca catccatcaa gacgaaccta cattccgaaa aagaatggta agctacgtcc
     8461 actaggtatt ccaacatttg atgataagct cttacaacag gttatcaaaa tgattctgga
     8521 agctatctac gagggacagt tcgaatcttc ttctcatggt tttcgtccga ataaaagttg
     8581 ccatacagca cttactcaaa ttcagaaaac ctatactgga acaaaatggt tcattgaagg
     8641 agacatcaaa tcattctttg ataatatcaa ccatgatgtg atgattcaga ttctgcgaga
     8701 aagaattaca gatgagcgat tcttacgcct gattcgtaag ttcctcaatg caggatatgt
     8761 ggaagactgg aaattctata aaacctactc aggaactcca caaggaggaa taatcagtcc
     8821 aattctagcc aatatctatt tggataagtt tgataaatat atgacagatt atgtgaagaa
     8881 cttctgtgaa gggaaatacc gtaaacgcat cccagaatat cgccaaaatg agattgcatt
     8941 ggggaaagca agaagaacac tagagtgtgc ttcgacggaa aaccaacgcc aagaagccat
     9001 tcaacgtatc cgtcaattag aaaaagaaag agtattgatt cctcatagcg acccgatgga
     9061 tagtagcttt aagcgtctaa cctatacccg atatgcagat gattttatct gtggtgtgat
     9121 tggaagtaaa gaggacgctc gtcgcattaa agcagacatt aaggactatt tgaaaacggt
     9181 tctcaaattg gaactctctg aagagaaaac cctgattacc aatgcaagag ataaagcgag
     9241 gtttctgggt tatcatctgt acattcgtca atccaattta gccaaacgtg attcggtagg
     9301 acgattggtc agaaattata caggtagatt ggttttagag gtttctattg aaacgattcg
     9361 agatagatta ctgtcctatg gggctatgaa aatgacttat catagaggtc acgaagtttg
     9421 gaaaccaaca gctcgttatt ttatgaaaga ctgtgatgat ttagagattt tagaacgcta
     9481 caatgcggaa ataagaggtt tttataacta ttattgtatc gctaacaaca gttctatcct
     9541 ccatcgcttt aagtatatta tggaatatag catgtataaa acctatgcga ccaagtatcg
     9601 tacgactaaa tctcacatca tccgtaaata taaaaaggat ggtcatttta ggatacagta
     9661 tattggacgt aaaggagata caatgacccg ttatttctat aatggaggtt ttagacgtca
     9721 aaagaaatct ttccttgaaa acgataattt gccaaatacg gcaaaatatt ttagccgaac
     9781 caatttaatt gatagattaa aagcaaaccg ttgtgagtac tgtcaagcta ccaatagtct
     9841 cattgaaatt catcatgtga gaaaactcaa ggatttaaag aaaaagactt tttgggaacg
     9901 tttgatgatt tctagacaac ggaaaacaat agcactgtgt aaagattgcc ataagaaatt
     9961 acattatggc aagttagatt gagtttataa atggagagcc gtatacattg aaaggtgtac
    10021 gtacggttcg ggggcgagta tttggaaacc tgccgtagta atacggtgag gcgctggata
    10081 cttagcctac agcgaaatgg tagattgatt cgtcagggaa acatgcacca ggaggtagat
    10141 atttatcact atattactaa agggagcttc gacaattatc t
//
LOCUS       DATUCG010000092         9976 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.094, whole genome shotgun sequence.
ACCESSION   DATUCG010000092 DATUCG010000000
VERSION     DATUCG010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9976
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.094"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            27..6095
                     /locus_tag="VCA20_001744"
     CDS             27..6095
                     /locus_tag="VCA20_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941479.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEP1835525.1"
                     /translation="MRVYSKELFSKKNRYSIRKLTVGAASVIVGISLFSGGTVLAEGT
                     ALADTPVETIENNDVSNTNEGVVDQGSSQTDEVVATDIEAVRTELNFAQSESQEVAAE
                     WTPASTPAGTVEVVEEEGIRYNRLSSVSDQDNGASTGTFTSPDLKMNEDGSAAISLDL
                     IERSEAEKGRFGVYLNHTNHTKHILVGYDKQGWFWEYKSPVATNGGTWYSGTRVDEPV
                     KDTAYRLNVNLTTGGQLSASIVSADNRETALFNAIQIPAEVLADLRSSNAAKLKVTSY
                     GADRTIVDVKATNQTGLNSDEANVDEQPVEETPTPADIQPDWRSTSAIKGIVAIKEEG
                     GVRYNALSSTTQNNNGDSIAVFEKAGLEGSPEGNVNLNLEFVERSENNQGRFGVFVKY
                     KDVNNFLFVGYDNLGWFWEYKVNGTGNWLRERTVTTSPVKDSKNTLSISYKSDGQLNA
                     TNNDVQLFDTLNLETAVKEALSAEKRVALKLGTFGQQLTKVDIKADDQTGVTPADEKE
                     ETPTTAFVVNDAAVAYDELNSGELTAKIDRAFPRVREYTFNGNKVFGQVHSLNSLRIN
                     NVKLTPTVTYNKVDDKTAHYVLDVVDEANKINASIKVQIKVEGKELHFDVLEVNNRNQ
                     VTYGAEIDDVAKLIQTIAFDENNLVSVASHQTDAKFDGSRMSTHTHRTGDEHIQLQDG
                     VNYRRQGYMYGFVSTDQFAAAVWSNSQNSYGGGASDFTRLTTNSHRYTVDGETGVHLG
                     IASSPWRWEGAHNGVVYPEYTLELPSAKVVFAADANNDNKVDWQDGAIAYRDIMNNPK
                     GHEYVPELVAYRIAMNFGSQAQNPFLMTLDGIKKIALHTDGLGQSILLKGYGSEGHDS
                     GHLNYADIGKRIGGTEDFKTLIEKSQPYGAKLGIHVNASETYPESQYFSEEILRRQAN
                     GDYAYGWNWIDQGINISGHYDLAHNRLKRWEDLKKELGDGLDFIYVDVWGNGQSGGEN
                     AWETHVLAKEINMQGWRNAFEWGYAGEYDSTFQHWAADLTYGGFTLKGINSNIARFIR
                     NHQKDSWVGDYPAYGGAANYPLLGGYDMKDFEGWQGRSDYNGYITNLFANNLMTKYIQ
                     HFQVTNWKNGDPVRMTDNGETYTWTPEMEITLKDKSDNTLVLTRKSNDVNNAGYRQRT
                     VTLNGRKIQDGAAYLVPWNWDANGNDLTSDKHKMYYFNEEAGATTWPLPSDWTGNKVY
                     LYKLTELGKTDVQELAVTNGQITIQADANVPYVLYKSPQTNPEMSWSDGMHIYDQGFN
                     SGKLDHWTIDGDTSKATLVKSQGANDMLRIQGNTSKVSLSQKLTGLKPNISYAAYVGV
                     DNRSTAKASITVNTGSSEVTNYTNESIALNYVKAYAHNTLPQNATVEDTSYFQNMYVF
                     FTTGDNVDNVHLTLSRDADAAATYFDEIRIFENDSTMFAGGHNTTAGTFKQNFESVPQ
                     GIFPFVIGGIEGVEDNRTHLAEKHAPYTQRGWNGKKVDDVIEGTWSLKTNGLTARNSI
                     VYQTIPQNFRFEAGKTYRVSFDYEAGSNGTYAFAIGEGRYNNDAGSLTLNPLNNSWED
                     SDKAKKASFLVTGADSGNTWVGIFSTRRGGDTKGDTGGNANFRGYNDFMLDNLVIEEV
                     TLTGHLLTTDYYNLNTPVLNENYTQASLVPYKEALLAVSAAPEDISIEDARTLIATAN
                     TARQNLQVKKTAITNEDIGLAQANAQEGEELAKAFDGNPSTIWHTPWNGRHINEPATV
                     NLRRPVDIQRFEYVPRQSGRNGILKALTLVITDDQNVEHTFTGADWSATSATKTIDFG
                     KTIKAKKIVITGTASYGDTADQFMSAAEFRFLTPVQEEATLDKAAYEAALAAARSAGK
                     TALVKEVEDFMASVEATNLLTANILDATVARLNASETPENPGNTTGTTTETPANPGTV
                     EQPVDEPVKQDEDVVTAKGESTKADPLQLGELPPLVIAKGDSVKAEPLPAFDGGLVPN
                     YAPTAAALPSIDPASLAKEAASPKPAKATSATLPQTGETTEQGLLLAAGLVGLMAMAA
                     RRRRFE"
     gene            6541..7608
                     /gene="dinB"
                     /locus_tag="VCA20_001745"
     CDS             6541..7608
                     /gene="dinB"
                     /locus_tag="VCA20_001745"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEP1835526.1"
                     /translation="MLIFPLINDLSRKIIHVDMDAFFAAIEVRDNPALKGKPVIIGAD
                     PRLSGGRGVVSTCNYEARAFGIHSAMSSKEAYERCPQAIFISGNYEKYQEVGRQVREI
                     FHRYTDLVEPMSIDEAYLDVTENKLGTSSAVKIAKLIQYDIWNELHLTASAGVSYNKF
                     LAKIASDMEKPHGLTLILPEDAVGILASLPVEKFHGVGKKTVERLHEMGVYTGQDLLD
                     VPEMVLIDRFGRFGFDLYRKARGISNSPVKVDRVRKSVGKERTYRKLLYREEDVLKEL
                     ISLCQRVAVSLKRNGKKGRTIVLKVRYGDFSTLTKRHSLEVYTDQMETIEKEVRQLIE
                     EIGKIEKGIRLLGVTVTNFQT"
     gene            7651..8070
                     /locus_tag="VCA20_001746"
     CDS             7651..8070
                     /locus_tag="VCA20_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEP1835527.1"
                     /translation="MQISSRFTIASHILVLLALEGEKEKQTSTSIAGSVGVNPVIIRN
                     ILSQLKEAGLVEVARGVGGARLAQGPDQITLLHVYQAVELFGEKGQLFAFHEQPNPSC
                     QVGRNIHTLLDSRLGNAQSALENELAKTRLADLLAEL"
     gene            8309..8934
                     /locus_tag="VCA20_001747"
                     /pseudo
     CDS             8309..8934
                     /locus_tag="VCA20_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646349.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
     gene            9089..9952
                     /locus_tag="VCA20_001748"
     CDS             9089..9952
                     /locus_tag="VCA20_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HEP1835528.1"
                     /translation="MSHLQYTAKSHHLQWNVCQLSQICHHFYQNYCPDSFKHRRNVGL
                     AKVSDESLLVLLLLQAELGMTSQRHFYKICHLFPCGRLLERSRFNRRSKQLIWLIQFI
                     RKAMSEMLPSDKLAIIDSFPLPLCQPVRNHRVTIFKGLADIGYNASKHLWFYGFKVHM
                     LVALSGYILNYVVTPASVHDIKVVYELLEGCKQSVILGDLGYLSSELKKDLEQQGYHL
                     WTPFRKNMTGSEEHNNWKVMAMRRTIETRFSELCRLFDIEHTLTRGIAGLQLRMEQII
                     LAHNLRYFEMN"
BASE COUNT         3030 a         1945 c         2268 g         2733 t
ORIGIN      
        1 aacaaaaggt aaaacggagg cagacaatga gagtttactc taaggaatta ttttctaaga
       61 agaatagata ttcaattaga aaattgacag taggtgcggc gtcagttatc gtaggaattt
      121 ctctattttc aggtggaaca gtattggcag agggaacagc gcttgccgat actcctgttg
      181 agacgataga aaacaacgat gttagcaata caaatgaagg agttgtagat caaggtagct
      241 cccaaacaga cgaggtagtt gctacggata tagaagcagt acggacggaa ttgaattttg
      301 cccagtcaga atctcaagaa gtcgctgcgg aatggacgcc agcctctaca ccggctggta
      361 cagttgaggt cgttgaagaa gaaggaattc gttacaaccg tttgtcttct gttagtgacc
      421 aagataatgg tgcttcaaca ggtacattta ctagcccaga tttgaagatg aacgaagatg
      481 gcagtgcagc gatttcccta gacctgatcg aacgttcaga agctgaaaaa ggtcgttttg
      541 gggtgtattt gaaccatacg aaccacacca aacatattct ggttggttat gataagcagg
      601 gatggttctg ggagtacaag tctcctgtgg ctaccaatgg tggaacttgg tatagtggca
      661 cacgtgtgga tgagccagtg aaagatacgg cttaccgtct caatgtcaat ctgactacag
      721 gtggccagtt gtctgcatcc attgtgtcag cagataaccg tgaaactgct ctcttcaatg
      781 ccattcaaat tccggcagaa gttttggcgg accttcgctc aagcaatgct gctaaattga
      841 aagtaacaag ctatggtgca gatcggacca tcgtagatgt taaggcaact aaccaaactg
      901 gtctcaacag tgatgaagca aatgttgatg aacaaccagt tgaagaaacg ccaacaccgg
      961 ctgatatcca accagactgg agatctactt cagctatcaa aggaatagtt gccattaagg
     1021 aagaaggtgg agttcgttac aatgcacttt cttcaacgac acaaaacaat aatggagata
     1081 gcattgccgt ctttgaaaaa gctggattgg aaggtagtcc agaaggcaat gtcaatctaa
     1141 acttggagtt tgtagagcgt tctgaaaaca accaaggccg ttttggagtc tttgtcaaat
     1201 acaaagatgt taacaacttc ctctttgttg ggtatgataa tttaggctgg ttctgggagt
     1261 acaaggttaa cggtacagga aactggctac gtgaacggac agtcactaca tctcctgtca
     1321 aagatagtaa aaatactcta tcgatttcat acaaatcaga tgggcagttg aatgctacaa
     1381 acaatgatgt acagttgttt gatacactaa acttggaaac agctgtgaaa gaagccttgt
     1441 cagctgaaaa acgagttgct ttgaaattag gtacatttgg tcagcaatta accaaggttg
     1501 atataaaagc agatgaccaa acaggtgtga cccctgcaga tgaaaaagaa gaaacaccta
     1561 caacagcttt tgtggtcaat gatgcggcag ttgcctatga tgaactcaat tcaggtgaat
     1621 tgactgccaa gattgaccgg gccttcccac gtgttagaga atataccttc aacgggaaca
     1681 aggtatttgg acaagttcat tctctcaatt cactacgcat caacaatgtg aaattgacgc
     1741 caaccgttac ctataacaaa gttgacgaca agaccgctca ctatgtcttg gatgttgtgg
     1801 atgaagctaa caaaatcaac gcttctatca aggttcaaat caaggtagaa ggtaaagagc
     1861 ttcactttga tgttttggaa gttaataacc gcaaccaagt gacctatgga gcagagattg
     1921 atgatgttgc caagttgatc caaaccattg cttttgatga aaataacttg gtttcagtcg
     1981 ctagccatca aaccgatgca aaatttgatg gaagccgcat gtcgacccac acccatcgta
     2041 caggtgatga gcatattcaa ttacaagatg gtgtgaatta ccgacgtcaa gggtacatgt
     2101 atggctttgt ttcaacagat caatttgcgg cagcagtttg gagtaattca caaaatagct
     2161 atggtggtgg ggcaagtgac tttacacgct tgacaacaaa tagccaccgt tatactgttg
     2221 atggtgaaac tggtgttcat ctaggaattg cttcctctcc atggagatgg gagggagccc
     2281 acaacggtgt cgtttatcca gaatatactc tcgaattgcc aagtgcaaaa gtagtttttg
     2341 cagctgatgc aaacaatgat aataaggtag actggcaaga tggggccata gcttaccgtg
     2401 acatcatgaa caatccaaaa gggcatgaat atgtaccgga actggtagct taccgtattg
     2461 ccatgaactt tggatcacag gctcaaaacc cattcttgat gaccttggat ggtattaaga
     2521 aaattgccct tcatacagat ggcttgggac aaagtatcct cttgaaaggt tatggtagtg
     2581 aaggtcatga ctctggacac ttgaattatg cagatattgg taaacgtata ggtggcacag
     2641 aagacttcaa gaccttgatt gaaaaatcac aaccatatgg tgcaaaacta ggtattcacg
     2701 tcaatgcatc tgaaacctat ccagaatccc aatacttctc tgaagagatt cttcgtcgtc
     2761 aggcaaatgg tgactacgcc tatggttgga actggattga tcaaggtatc aacatcagtg
     2821 gtcattatga tttggcccac aaccgtttga aacgttggga agatttgaag aaagaactgg
     2881 gtgacggatt agactttatc tatgtcgatg tgtggggtaa tggtcagtct ggtggcgaaa
     2941 atgcttggga aactcatgtt cttgccaaag aaatcaatat gcaaggttgg agaaatgcct
     3001 ttgaatgggg ttatgcaggt gagtatgact cgaccttcca acactgggca gcagacttaa
     3061 cctatggtgg ctttactctg aaaggtatca actctaatat cgcccgcttc atccgtaacc
     3121 accaaaaaga ttcatgggtt ggtgactatc ctgcatatgg tggtgctgca aactatccac
     3181 tgcttggcgg ctacgatatg aaagatttcg aaggatggca aggacgtagt gactataatg
     3241 gttacattac caacttgttt gccaacaacc tgatgaccaa gtatatccaa cacttccaag
     3301 taaccaactg gaagaatggt gaccctgttc gtatgacgga caacggagaa acctatactt
     3361 ggacgccaga gatggaaatc actttgaaag acaagtctga caataccttg gtcttgaccc
     3421 gtaagtcaaa tgatgttaac aatgctggct accgtcaacg gacagtaacc ctcaatggtc
     3481 gcaagatcca agatggcgca gcatacttag taccttggaa ttgggatgcg aatggtaatg
     3541 acctgaccag tgacaaacac aaaatgtact acttcaacga agaagcaggt gcaacaacct
     3601 ggcccctccc atcagattgg actggtaaca aggtttatct ctacaaactt actgagctag
     3661 gtaagacaga tgttcaagaa ttggcagtaa ccaatggtca aattaccatt caagctgatg
     3721 caaatgtgcc gtatgttcta tataaatctc cgcagacaaa tccagagatg tcctggagcg
     3781 acggcatgca tatctatgac caaggcttta actcaggtaa gctagaccat tggacaattg
     3841 atggtgatac tagcaaggct actttggtca aatcccaagg tgccaacgat atgctccgta
     3901 tccaaggcaa tacatccaaa gtaagtctta gtcaaaaatt gacaggtctg aaaccaaata
     3961 tcagctatgc agcttatgta ggggtagaca atcgcagtac agcaaaagct agcattacag
     4021 tcaatacagg cagttcagaa gttacaaact atacaaatga gtctattgca ctgaactatg
     4081 taaaagccta tgctcataat acattgccac aaaacgcaac ggttgaggat acaagttact
     4141 tccaaaacat gtatgtattc ttcacaactg gagacaatgt ggataatgta cacttgaccc
     4201 tatctcgcga tgcagatgct gcagcaacct attttgatga aattcgtatc tttgaaaatg
     4261 actcaacgat gtttgcagga ggtcacaata caactgctgg taccttcaag caaaactttg
     4321 agagtgttcc gcaaggtatt ttcccatttg ttatcggtgg tattgaagga gttgaggaca
     4381 accgtaccca ccttgcagaa aaacatgctc catacactca acgtggctgg aatggtaaga
     4441 aggtggacga tgtcattgaa ggcacttggt cactcaagac aaatggtctg acagctcgca
     4501 atagtattgt ttaccaaact attccgcaaa acttccgctt tgaagctggc aagacctatc
     4561 gtgtaagctt tgattatgaa gctggttcca atggaactta tgcctttgct atcggtgaag
     4621 gacgttataa caatgatgcc ggtagcctaa cgctgaatcc attgaacaat tcatgggaag
     4681 atagtgataa ggctaagaaa gcaagtttct tagttacagg tgcagattct ggcaatactt
     4741 gggttggtat cttctcaaca cgccgtggtg gtgatactaa gggagatact ggtggaaatg
     4801 ccaacttccg aggctacaat gactttatgt tagataatct ggtgattgaa gaagttacct
     4861 tgactggtca cttgttgaca acggattact ataacttgaa taccccagtc ttgaatgaaa
     4921 actatactca agcaagtttg gtgccgtaca aagaagcttt gttggcagtg tctgcagctc
     4981 ctgaggatat tagtatcgaa gacgctcgta ccttgattgc taccgccaat acagcccgtc
     5041 aaaatcttca agtgaagaaa acagcgatca caaacgaaga tattggcttg gctcaggcta
     5101 atgctcaaga aggtgaagaa ctagctaaag cctttgatgg caatccttct actatctggc
     5161 acacaccatg gaatggccgt catattaatg agccagcgac agttaacctt cgtagaccag
     5221 ttgacattca acgttttgaa tacgtgccga gacaatcagg ccgtaacggt atcttgaaag
     5281 cattgacatt ggtaattact gatgatcaaa acgtggaaca tacctttact ggtgcggatt
     5341 ggtcagcaac aagtgctact aagacaattg atttcggtaa gacaattaaa gctaagaaga
     5401 ttgtgattac aggtactgcc tcatacggtg atactgcaga tcaatttatg tctgctgctg
     5461 aattccgttt cttgacacct gttcaggaag aagccaccct tgataaggca gcatatgaag
     5521 cagcacttgc tgcagcacgt tcagcaggta agacagctct tgtcaaagaa gtggaagact
     5581 ttatggcttc tgtcgaagct acaaatcttt tgacagcaaa tatcttagat gctacagttg
     5641 ctcgcttgaa tgctagtgaa acacctgaaa atccaggcaa tacgacaggt actactaccg
     5701 aaacgcctgc aaatccaggc acagttgagc aaccagttga tgagccagtc aaacaggatg
     5761 aggacgttgt cactgctaaa ggagaaagta ccaaagcaga tcctcttcag ttaggggaat
     5821 tgccaccgct tgtgattgca aaaggtgata gtgttaaagc agaaccatta cctgctttcg
     5881 atggaggctt agttcctaac tatgctccta cagcagctgc tcttccaagt attgatcctg
     5941 caagcttggc gaaagaagct gctagtccaa aaccagcaaa agctacttca gctactttac
     6001 cgcaaacagg tgaaacaact gaacaaggct tgctcctagc agctggttta gtaggtctaa
     6061 tggctatggc ggctcgaaga agacgatttg agtagccatt ttagaaaaaa ataattagct
     6121 tgttctccaa atacattaga ggtaggtatc ggacctgcct cttttgtatt gtttatcgaa
     6181 ccttttcaca aacttatagc aaaatggaat tagagacaga gcacgtctaa gtttatacat
     6241 ttaaaaattc tatagtcgat tgaattagaa gcaggacata tataagtttg tcaatttgag
     6301 aactctaaag ttattttagg agtgggttat taacactata acttaaaatt gtctttttcc
     6361 taattcaatc cactataata tctaaaaatg cgaaagtaga agggttctat ctacatatga
     6421 ctctcctagt ctgattttga ttttcattga gtactacttg cacgttaatg ttcgctttat
     6481 gaaaaaattt gctagatagg ctataatatg tctagtagta aggagcagaa aggagtgtcc
     6541 atgttaatat ttcccctaat caacgattta tccagaaaaa ttattcatgt tgacatggat
     6601 gctttttttg ctgccattga ggtcagggat aatcctgctt tgaagggtaa acctgttatc
     6661 attggggctg acccaagact atctggcggt agaggagtgg tgtctacctg taattatgag
     6721 gcgcgtgcct ttgggattca ctcggctatg tcctcaaaag aggcctatga gcgttgtcct
     6781 caggcgattt tcatttctgg caattatgaa aaatatcagg aagttgggcg acaggttcga
     6841 gaaattttcc atcgctatac tgatttggtg gagcccatgt ctattgatga ggcttacttg
     6901 gatgtaacag aaaataagtt gggcaccagc tctgcggtta agattgccaa gcttatccaa
     6961 tacgatattt ggaatgaatt gcatctgaca gcttccgcag gtgtttccta taataaattt
     7021 ttggcaaaaa ttgcctctga tatggaaaaa ccgcacggtt tgaccttgat tctacctgaa
     7081 gatgctgttg gtattctcgc ctccctgcct gttgaaaaat ttcacggtgt ggggaagaag
     7141 acggtggaac gcctgcatga gatgggggtc tacacggggc aagatttgct agatgtacca
     7201 gaaatggttt tgatagatag atttgggcgt tttggcttcg acctttatcg gaaagcaagg
     7261 gggatttcaa attcgcccgt taaagtggac cgtgttcgta agtcagttgg aaaggaaaga
     7321 acttatcgca aacttcttta tcgggaggag gatgtcctga aagagctaat cagtctctgc
     7381 caacgggtcg cagtcagttt gaagcgaaat ggaaaaaagg ggcgaacaat tgttttaaag
     7441 gtacgttatg gtgacttttc taccttgacg aaacgtcata gtctagaggt gtatacggac
     7501 cagatggaaa cgattgagaa agaggttcgt caactaattg aagaaattgg gaagattgaa
     7561 aaagggattc gtttgctggg tgtgaccgtg actaattttc aaacttgagt tctgtttcag
     7621 tctatgatag actgaaaaaa aggagaagag atgcaaattt caagtagatt taccattgcc
     7681 agccatatct tggttctctt ggctctggaa ggagaaaaag aaaaacagac cagtactagc
     7741 attgcaggca gtgtcggggt taatcctgtc attattcgaa atattttgtc ccaactcaag
     7801 gaggctggtc tggtagaagt agcgcgtggt gttggaggtg ctcgcttggc acagggtcca
     7861 gaccagatta cacttttgca tgtctatcag gctgtggagt tatttggaga aaaaggtcaa
     7921 ttattcgctt ttcatgagca accaaatcct tcctgccaag taggtcgcaa tattcatact
     7981 ctgctagata gccgtttggg aaatgctcag tcggctttgg aaaatgaact tgctaagaca
     8041 aggcttgctg atctcttggc ggaactatag tcgttttatt aggtcaacgt aaggagaaac
     8101 tttgtttcct tatttcctac ttcaaacact ccactggact gttgaagtag tctgatgcgt
     8161 gttgctctag cactaaaaga ctagttagtg aatcatcctt gtgatgattt ttttaatttt
     8221 taggttgtaa taattgatgt tacaaccaaa atgatttata cttatgttgt aaacgaaacc
     8281 attacaatga aataagtgag gtatcaatat gaaaattgct attattgcag cgcatggtca
     8341 agcaggtcaa actattgcaa aggaagcagt aaaacgtggt catcaggtga cggctattgt
     8401 ccgttctgaa aacaagagcg tagcccagga agtgatccaa aaggatgctt ttgctcttac
     8461 caaagaagat ttggcaggct ttgatgtagt ggttaatgct tttggtgctt ggacaccaga
     8521 aaccttgccg gatcacgctc gtcttgctga gcatctagcg accatccttg ctggtagtcc
     8581 aacccgtctt ttagtagttg gtggtgcagg cagtctttat ttagacgaga gccagacagc
     8641 tatgctgaaa gatactccag atttccctgc tgaatacctg ccaatcgctg aggctatggg
     8701 tgctggtcta gacctctatc gtcaggcgac agcggtcaac tggacctata tagcccagca
     8761 gcagattttg atgcagaagg tcaaaaagca ggtgcctaca ctttggctgg cgaggttttc
     8821 caagtcaatg ctcaaggtga aagctatatt agttatgccg actatgctgt ggctttggtt
     8881 gacattgcgg aaaaaggtgg ctaccagcag gaacgaattt ccgtacttgc ataaaaattt
     8941 aaatggtcta aaagatagtt cccatcaagg aattatcttt ttatttttta cttgcatata
     9001 acccgtggtg ctaattcatt tcaaactaca ataaaaagcc caaatggact atactgtaag
     9061 cgacgaaaca tacaggaggt tagtccaaat gagccactta cagtatactg ctaaatctca
     9121 tcatttgcaa tggaatgtat gccaattatc acaaatttgt catcacttct accaaaatta
     9181 ttgcccagat tcctttaaac atcgacggaa tgttggctta gccaaagttt cagatgaatc
     9241 gctccttgtc ttgcttttgt tacaagctga actaggtatg acatcacaac gccatttcta
     9301 caagatctgc cacctctttc cttgtggtcg gttactagaa agaagtcgtt tcaatcgtcg
     9361 gtcaaaacaa ttgatttggc taatccaatt catccgaaaa gctatgagtg aaatgcttcc
     9421 atctgataaa ctagccatta tagatagttt tcctttgcca ctttgccaac ctgttcgcaa
     9481 tcacagagtt accattttta aaggattagc cgatattggc tacaacgcct ctaaacatct
     9541 ttggttctac ggcttcaaag ttcatatgct agtggcctta tcaggttata ttctgaacta
     9601 tgttgtcacg ccagcctctg ttcatgacat caaggtggtt tatgagctat tagaggggtg
     9661 taagcaatcg gttatcctag gtgatttagg ctacctgagc agtgaactca agaaggactt
     9721 agaacaacaa ggttaccatc tatggacgcc gtttcgtaaa aacatgactg ggtccgaaga
     9781 acataacaat tggaaagtaa tggccatgag acgaactatt gaaacacgtt tttctgaact
     9841 gtgtcgtctc tttgacatcg aacatacatt aactagaggg atagccggac tacagttacg
     9901 aatggaacaa attattctag ctcataactt acgctatttt gaaatgaact agcaccacgg
     9961 gtttgcatat agtata
//
LOCUS       DATUCG010000093         9900 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.095, whole genome shotgun sequence.
ACCESSION   DATUCG010000093 DATUCG010000000
VERSION     DATUCG010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9900
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.095"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            186..1412
                     /locus_tag="VCA20_001749"
     CDS             186..1412
                     /locus_tag="VCA20_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HEP1835529.1"
                     /translation="MFCTYKIGISAEEHDSFVKQSPQANLLQSSQWATVKSEWRNERL
                     GFFKDGKLVAVASLLIRRLIVPFGFTMIYIPRGPVMDYEDAELVSFVFKSIKEFALRE
                     RSVVIKFDPFIRLSSKTITGDREEELNVLAVKDRIVDLGGIWSGRVQTMSATIQPTCH
                     AVLYSESFSEESLNKRVRQNIRSARNKGIEIRIGREELLRDFSELLKKTEERKSIRLR
                     GREYYQKILNAYPENSYIVVAYLDLAKRYEQLSYLEEKLVKEAMTFTSTTKQSKIDNY
                     KKETRRINTELEMINSLIRNGSKLVPLAGTLTIDFAGTSEIIYAGMDEKFKHYQPALL
                     TWVETAKYAFERGVKWQNLGGIEPTLDGGLYHFKSHLNPVVEEYIGEFDLIVSPILYK
                     IFQVSLEIRKKLRNKK"
     gene            1412..2584
                     /locus_tag="VCA20_001750"
     CDS             1412..2584
                     /locus_tag="VCA20_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HEP1835530.1"
                     /translation="MTFCQITKEEFLKHCEQVNEKSFFQSVEMAGLLSKRGYDVRYAG
                     FKNSQSEIVISTLLFCKKMAGGLYMELNSGPLVTDDAYLEEFYQSLKNYAKREGVLEL
                     VVKPSQTYQLFDSVGNPISEADTQLIEKMVQVGFKHDGLQTGYPNGEPVWHYVKDLQG
                     YSTTSLLQSYNKNSKRNIKNSESYGIRIRSINHKELSAFKKIIEETGARQGFEDRDSG
                     YYNSLFESFGTQADFLVAELDPKASLKQIEKEIETSVRNSKQYAQQVQKLLKKQKVVQ
                     NILNQTQTAPIFLACALIIYDVNEATYLFGGSYSEFQSLSAPFLLQYEAMQRAIKRNL
                     KRYNFFGIQGIFDSSDGVLRFKQNFNGYILRKAGTFRYYPHPIKHKIIQIIKEIVK"
     gene            2614..2754
                     /locus_tag="VCA20_001751"
                     /pseudo
     CDS             2614..2754
                     /locus_tag="VCA20_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194216.1"
                     /note="cleaves off formyl group from N-terminal methionine
                     residues of newly synthesized proteins; binds iron(2+);
                     incomplete; partial in the middle of a contig; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
     gene            2913..3179
                     /locus_tag="VCA20_001752"
                     /pseudo
     CDS             2913..3179
                     /locus_tag="VCA20_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194216.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
     gene            3692..5791
                     /locus_tag="VCA20_001753"
     CDS             3692..5791
                     /locus_tag="VCA20_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEP1835531.1"
                     /translation="MNNNFNNFNNMDDIFNQLMGNMGGYSTERRRYSINGREVTPEEF
                     AMYRQTGRLPQTEEVAQTQAKGQIKSDGILAKLGRNLTQDAREGKLDPVIGRNKEIQE
                     TSEILARRTKNNPVLVGDAGVGKTAVVEGLAQAIVNGDVPAAIKNKEIISIDISGLEA
                     GTQYRGAFEENVQNLVDEVKKAGNIILFFDEIHQILGAGSTGGDSGSKGLADILKPAL
                     SRGELTVIGATTQDEYRNTIHKNAALARRFNEVKVNAPSAEDTYQILKGIKPLYEAHH
                     NIELPDEVLRAAVDYSVQYIPQRSLPDKAIDLIDVTAAHLAAQHPVTDIQTLEAEMAE
                     AKQLQLEAAEKEDYEKALNEKVRIDKLQKQIDNHTEQQKVVATVNDVAQAVERMTGIP
                     VSQMGASDIERLKELKNRLAANVIGQDDAVEAVSRAIRRNRAGFDDGNRPIGSFLFVG
                     PTGVGKTELAKQLALDLFGNKDAIIRLDMSEYSDRTAVSKLIGTTAGYIGYDDNSHTL
                     TERIRRNPYSIVLLDEIEKADPQVITLLLQVLDDGHLTDGQGNQVNFKNTIIIATSNA
                     GFGFGMAEGEENQDIMDRIAPFFRPEFLNRFNAVIEFKHLDKDDLKAIVELLLAQVNN
                     TLAKKGIQLMVTEAAKEFLMEEGYDQAMGARPLRRVIENQIRDKVTDYYLDHVDVKYL
                     EADVVDGSIQIKEQSFA"
     gene            5974..7482
                     /gene="malQ"
                     /locus_tag="VCA20_001754"
     CDS             5974..7482
                     /gene="malQ"
                     /locus_tag="VCA20_001754"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019312918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEP1835532.1"
                     /translation="MTNRTSGILMHITSLPGKFGIGTFGQSAYDFVDFLVETKQTYWQ
                     ILPLTTTSYGDSPYQSFSAIAGNTHLIDFDLLVEDGLLATEDFQDVNFGDNPEKVDYA
                     LIYQVRRPILEKAVQAFLQDDKKKAAFLEFEKANSSWLTDYAEFMAIKEYFGNKALQE
                     WEDKKVVARNEEALDKYRLELANQIDYYKVTQYFFFSQWKQLKEYANKHRIKIIGDMP
                     IYVSADSVEVWTKPQLFKLDSERKPLYVAGVPADNFSADGQLWGNPIYDWPEHEKTGY
                     NWWIYRIHESFKLYDVLRIDHFKGFSDFWQVDGKAEVAKVGTWEPGPGYNLFKAVKET
                     LGDLPIIAEDLGNIDAKARKLLVDCGYPGMKILEFGFFDVTGKSIDAPHRCIPNSVAY
                     TGTHDNEVVNGWYNNLDPEQQEYVDAYSNRKPIEKVSQAMLRMLFATVSDTAIATMQD
                     VLDLGEESRMNMPSTIGGNWEWRMKAEDLTQERKDFLTKMTLLYQRGNENHD"
     gene            7475..9739
                     /gene="glgP"
                     /locus_tag="VCA20_001755"
     CDS             7475..9739
                     /gene="glgP"
                     /locus_tag="VCA20_001755"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HEP1835533.1"
                     /translation="MTKFTTFAETNTSKKLADLTNEEIYLQLLNYVKDAAASKPKNTG
                     KRKVYYISAEFLIGKLLSNNLINLGVYKDVQAELAAAGKSLAQVEDVEPEPSLGNGGL
                     GRLASCFVDSMSTLAINGEGVGLNYHCGLFRQVFKKNEQEAEPNFWIENDSWLIPTTI
                     SYDVPFKNFTLKSKLDRLDILGYKKETKNYLNLFDIESVNYDLITDGISFDKTDIKEN
                     LTLFLYPDDSDKNGELLRIYQQYFMVSNAAQLLIDEAIERGSNLHDLADYAYVQINDT
                     HPSMVIPELIRLLTEKHGIEFAEAVAIVKNMTGYTNHTILAEALEKWPLEFLEEVVPH
                     LVDIIKELDALIRAEIKDPAVQIIDESGRVHMAHMDIHFSNSVNGVAALHTEILKNSE
                     LKAFYELYPEKFNNKTNGITFRRWLEFANQDLADYIKELIGDEYLTDATKLEKLLAFA
                     DDKEVHVKLAEIKHNNKLALKRYLKDNKGIELDENSIIDTQIKRFHEYKRQQMNALYV
                     IHKYLEIKNGNLPKRKITVIFGGKAAPAYIIAQDIIHLILCLSELINNDPEVNKYLNV
                     HLVENYNVTVAEKLIPATDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIA
                     ELAGMDNIYTFGKDSDTIIDLYDKAGYVSADYYNGDANIKRAVDFIVSDEVKALGNEE
                     RLGRLHHELISKDWFMTLIDLAEYIEVKEQVFADYEDQDSWNKKVVHNIAKAGFFSSD
                     RTIEQYNQDIWHSN"
BASE COUNT         3222 a         1837 c         2096 g         2745 t
ORIGIN      
        1 gtgaaaacta ggcttttttt actgaaaaca ggacaaaata cttgacaaaa ccgctgatat
       61 actaagcata tcaggacttg acctatcggg caagccctga tatactaagc atatcaggac
      121 ttgacctatc gggcaagccc ttcctaatta gcgttaaaag taattatgaa caaggggaat
      181 aatcaatgtt ttgcacttat aaaataggta tttctgctga ggaacatgat tcatttgtaa
      241 agcaatcacc acaggccaat cttttgcaga gtagtcagtg ggcaaccgta aaaagtgagt
      301 ggcgaaatga gagactaggc ttttttaaag atgggaaatt agtggcagtt gcttctcttc
      361 ttatcagaag gttgatagta ccatttggtt ttacgatgat ttatataccg agaggtcctg
      421 taatggatta tgaagatgct gaattagtat catttgtatt taagtccata aaagaatttg
      481 ccctgcgaga acgcagtgtt gttataaaat tcgatccatt tattcgacta tctagtaaaa
      541 ctataacagg agatagggaa gaagaattaa atgtacttgc cgtcaaagat aggattgttg
      601 atttgggagg tatttggtct ggtcgagtgc agaccatgtc ggctacaatc caacctactt
      661 gtcatgcagt attatatagc gaatccttct cagaggaatc actaaataaa cgagttcgac
      721 aaaatattcg gtcggcgcgt aataaaggta ttgagattcg aattggtcgt gaggaattgt
      781 taagagattt ttcagagcta ttaaaaaaga ctgaggagcg caaatctatt cgcttgcggg
      841 ggagagagta ttatcaaaaa atattgaatg cctatccaga aaattcttat attgtagttg
      901 cctatttgga tttagcaaag agatatgaac agttatctta tcttgaagaa aaattagtaa
      961 aagaagccat gactttcacc tccactacaa aacaatcgaa gattgacaat tataaaaaag
     1021 aaacccgaag aataaataca gaattagaaa tgataaattc tctgattcgt aacgggagta
     1081 agttagttcc attggctggt actttgacaa ttgattttgc aggaacatct gaaattatct
     1141 atgctggtat ggatgaaaag tttaaacatt atcagcctgc tttactaaca tgggtggaga
     1201 cagcaaagta tgctttcgaa cgaggtgtga aatggcaaaa tttaggagga atagagccaa
     1261 ctttagacgg aggcctttat cactttaaat cacatttgaa tccagtagta gaagagtata
     1321 ttggggaatt cgacctgatt gtttcgccaa tattatataa gatttttcaa gtttcactgg
     1381 aaattagaaa aaaattaagg aataaaaaat aatgactttt tgtcaaataa ctaaagaaga
     1441 atttttaaag cattgtgaac aagtaaatga aaaatcattt tttcaatctg ttgagatggc
     1501 tggattacta tccaagcgag gatatgatgt tcgctatgct ggttttaaaa attcacaatc
     1561 agaaatcgtt atttctactt tgcttttctg taagaagatg gctggtggtt tgtatatgga
     1621 attgaattct ggaccgctgg ttaccgatga tgcttattta gaggaatttt atcaatcctt
     1681 aaaaaactat gctaagcgtg aaggcgtact tgaattggtg gtcaagcctt cccaaaccta
     1741 tcaactcttt gatagtgtag ggaatccaat tagtgaagca gatactcaac taattgagaa
     1801 gatggtgcaa gttggattta aacatgatgg tttacaaaca ggttatccaa atggagagcc
     1861 tgtttggcat tatgtgaagg atttgcaagg gtattcaaca acgtcgttat tgcaatccta
     1921 caataaaaat agtaaacgaa acataaaaaa ttcagaaagt tacggtatca ggattcgtag
     1981 tatcaatcat aaggagcttt cagctttcaa aaaaataatt gaggaaactg gagctagaca
     2041 aggctttgaa gatagagata gtggttacta taacagtctt tttgaaagct ttggtacaca
     2101 ggcagatttt ttagttgcag aacttgaccc gaaagccagt ctaaagcaaa ttgaaaagga
     2161 aatagaaaca agtgttagaa attcaaaaca gtatgcacag caggtacaga aattgttgaa
     2221 aaaacagaag gtggttcaaa atattttaaa tcaaactcag acagctccta tttttttggc
     2281 atgtgcactg ataatttatg atgtgaacga ggcaacttat ctttttggtg gctcatattc
     2341 agagtttcaa agcctatctg cacctttctt acttcaatat gaggcgatgc aacgtgcaat
     2401 aaaacgaaat cttaagcgtt ataatttctt tggtattcaa gggatattcg acagctcaga
     2461 tggtgttctt cggtttaaac agaactttaa tggttatatt ttgagaaaag ctggtacttt
     2521 tcgttactat cctcatccga taaagcataa aataattcaa attatcaaag aaatagtaaa
     2581 ataatactat atactttatc gaaagaggtt cccatgattc aacctattat gaaagatatt
     2641 ttcttcttgc agcagaagtc tgagcctgcg actcagctag atgtacaggt cggtcaggat
     2701 ttgcaggata ctttgtcagc caacgctcac gcctgcgtgg gcatggcggg gagtgggaaa
     2761 gaactcgaca agtttaaaaa gagttcgtct tcccaccccc gcacagttga ttaggtcaga
     2821 tttggagtgt gaaacacgaa caaatctgcc aatcaaccac tgcgctgaga tgttgacacg
     2881 aactctgaga agcgaggctg gactttctgc ccagcctcgt caaaaagcgg attatcatcg
     2941 tcaacatggg ttttaccaac ctggtcatgt acaatcccgt cctcatcagc aaggccaagc
     3001 cctatcagac cgaggagggt tgcctgtcgc tggaaggtag ccgccccacc actcgctatc
     3061 aggagattga agtggagttt tttgatgctt cttggaaaaa gcagagccta aagctgatag
     3121 acttccaagc ccagattgtt cagcatgaac tagatcattt ggaagggatt atcatctagt
     3181 agctaccaaa tctaccgtca gatagatttt ttatcaaaaa gtcaaaaaag gtcaaacaaa
     3241 actgttgact tttactgact aatggtgtag aataagattc gtaaggtaaa gggccttaca
     3301 aattctttga acaaaaggtc agtttaggtc aaacgagaat ggtttgactt actcttgaac
     3361 ctattggtca gtaaaagaca aacttctgag gaggttgcta tgaaagacag gattataccc
     3421 tattagcgtt atttagttta tcttttatta ctgtggcgaa tggggagtgg gaaagaactc
     3481 gaccagttta aaaagagttc gtcaacaagt ccttatttcc agttgttgag ctgaaacagt
     3541 ctaaccccag actcccactc ccgcacagct caaaaggttc ctcaaacctt ttgagctagt
     3601 actaaaagca aactaaaaga ctaaatcaat ttttttaaaa caaaggtcag taaaagttaa
     3661 aaacaaataa aacttaaacg aggtaaaaac tatgaacaac aatttcaata actttaacaa
     3721 tatggacgac attttcaatc aactcatggg caacatggga ggctacagca cagaacgtcg
     3781 ccgttattct atcaatgggc gtgaagtgac accagaagaa tttgctatgt accgtcagac
     3841 tggtcgcttg ccacagacag aagaagtggc tcaaacacag gccaaaggtc agataaagtc
     3901 agacggaatt cttgcaaaac ttggtcgcaa tttgacacaa gatgcgcgtg aaggtaagtt
     3961 ggatccagtc attggacgaa acaaggaaat ccaagaaacc tctgaaatcc tagctcgccg
     4021 tacgaaaaat aatcctgttt tggtcggtga tgctggtgtt ggtaaaactg cggtagtaga
     4081 aggattggct caagccattg tcaatggtga tgtgccagca gccattaaga acaaggaaat
     4141 catctccatc gacatttcag gcttggaagc tgggactcag tatcgtggag cttttgagga
     4201 aaatgtgcaa aatcttgtgg atgaagtcaa aaaagctgga aatatcattc tcttctttga
     4261 tgaaattcat cagattttgg gagccggtag cactggcgga gactcaggtt ccaagggttt
     4321 agcggatatt ctcaaaccag ctttatcccg tggggaattg acggttatcg gtgccactac
     4381 gcaggacgaa taccgcaaca ccattcacaa gaacgccgct ctggctcgtc gtttcaatga
     4441 agtcaaggtt aatgctccgt ccgcagaaga cacctaccag attttgaaag ggattaagcc
     4501 gctttatgaa gcccaccaca atattgaatt gccagatgag gttttgagag ctgcggttga
     4561 ttattccgtc caatatattc cacaacgtag cttgcctgat aaggctattg acctgatcga
     4621 tgtgaccgct gcacacttgg ctgcacaaca tcctgtgaca gatattcaga ccttggaagc
     4681 agaaatggct gaggcaaaac agttgcagtt ggaagcagct gaaaaagagg attatgaaaa
     4741 agccttgaat gaaaaagtcc gtattgataa gctacaaaaa cagattgata atcatacgga
     4801 gcaacagaaa gtggtggcga ccgtcaatga tgtggctcag gcggttgagc gtatgacagg
     4861 aattccagtc tctcaaatgg gagcttctga tattgaacga ctcaaggaat taaaaaatcg
     4921 tctggcagct aacgttatcg gtcaagacga tgcggtggaa gctgtatctc gtgccattcg
     4981 tcggaatcgt gcaggttttg atgacggcaa ccgtccgatt ggttccttcc tctttgtcgg
     5041 tccgacaggt gtgggtaaga cagagctggc taagcaacta gcactagact tgtttggtaa
     5101 caaggatgcc attattcgtt tggatatgtc tgaatacagc gatcggacag ccgtttccaa
     5161 attaatcggt accacagctg gttacattgg ctacgatgac aattcccata ctcttaccga
     5221 gcgtatccgc cgtaatccgt actcaatcgt tcttctggat gagattgaaa aagctgatcc
     5281 acaagtgatt acccttctct tgcaagtact agatgacggg catttgactg atggacaggg
     5341 caaccaagtt aactttaaaa ataccattat tatcgcgact tctaatgctg gttttggttt
     5401 tggaatggca gaaggtgaag aaaatcaaga catcatggac cgcatcgcac cattcttccg
     5461 tccggagttc cttaatcgtt ttaacgcagt tatcgaattc aagcacttgg ataaagatga
     5521 tctcaaagcc attgtagaat tactcttggc tcaggtcaat aataccctgg cgaagaaagg
     5581 catccaatta atggtaacgg aagctgccaa agaattcttg atggaagaag gctatgacca
     5641 ggccatggga gcccgtcctc tgcgccgtgt gattgaaaat cagattcgag acaaggtaac
     5701 agattactat ttggatcatg ttgatgtcaa atatctggaa gcggatgtgg ttgatggaag
     5761 catccaaata aaagagcaaa gctttgctta aaaatagtga agaatttgaa aaatcggata
     5821 gaaatatctg attttttctt ttttgttatc cgctaacaaa tgctataaaa taggctttct
     5881 agagcacatt aaaacttttt tcataaaatc cttgacaagt gcaaacgttt gcgttaaact
     5941 atattcgtaa ccgacagaaa ggaaaacgtt tttatgacaa atcgtaccag tggaattttg
     6001 atgcatatta cctcacttcc aggtaagttt ggtattggta cttttggaca gtctgcctat
     6061 gattttgtag atttcttggt ggaaaccaag caaacctact ggcagatttt accactaaca
     6121 actacaagct atggagattc tccttaccag tctttctctg ctattgcagg aaatacacac
     6181 ttgatagact ttgacctctt ggttgaagat ggtttattgg caacagaaga tttccaagat
     6241 gtaaattttg gagataatcc tgaaaaagta gactatgctc ttatctatca agttcgtcga
     6301 ccaattttgg agaaggcagt tcaagcattt ttacaagatg acaagaagaa agctgctttt
     6361 ctagagtttg aaaaggctaa ctcatcttgg ttgacagatt atgctgagtt catggctatc
     6421 aaggaatact ttggtaataa agctctccaa gaatgggaag ataaaaaagt tgtagctcgc
     6481 aacgaggaag ctcttgataa gtaccgctta gaattggcaa atcaaatcga ctactacaaa
     6541 gtcacacagt atttcttctt cagccaatgg aaacaattga aagaatatgc caacaaacac
     6601 cgcatcaaaa tcatcggaga catgccaatc tatgtttctg ctgatagcgt tgaagtgtgg
     6661 acaaaacccc aactattcaa attggacagt gaacgcaagc cgctttatgt agcaggtgta
     6721 ccagctgata acttctcggc agatggtcaa ttatggggaa atcctattta cgattggcca
     6781 gaacatgaaa aaacaggcta taactggtgg atttatcgta tccatgaaag tttcaaactt
     6841 tatgatgtcc ttcggattga ccatttcaaa ggtttctcag acttctggca agttgatggt
     6901 aaggcagagg tggcaaaagt tggaacttgg gaaccaggtc ctggctacaa tctcttcaag
     6961 gctgtcaaag aaacacttgg agaccttcca atcattgcag aagaccttgg aaacattgac
     7021 gctaaagctc gcaaattgct tgtagattgt ggctatccag gcatgaaaat cttagaattt
     7081 ggtttctttg atgtaacagg caagagtatc gatgctccac accgttgcat tccaaattca
     7141 gttgcctaca ctggaactca cgataatgag gttgtcaatg gctggtacaa caatcttgat
     7201 ccagaacaac aagaatacgt ggacgcctac agcaaccgta agccgattga aaaagttagc
     7261 caagctatgc tccgtatgct gtttgcaaca gtgagtgata cagctattgc aaccatgcaa
     7321 gacgtcctag acttgggtga agaaagtcga atgaatatgc cgtcaactat cggtggaaac
     7381 tgggaatggc gcatgaaagc tgaagacttg acccaagaac gcaaagactt cttgacaaaa
     7441 atgacattac tataccaacg aggaaatgaa aaccatgact aaatttacaa catttgcaga
     7501 aacaaatact tcgaagaaat tagctgattt gacaaatgaa gaaatctatc ttcaactgtt
     7561 gaactacgta aaagatgcag cagcttcaaa accaaaaaac acaggaaaac gtaaagttta
     7621 ctatatctca gcggagttcc ttatcggtaa actcttgtca aacaacttga ttaacttggg
     7681 cgtgtacaaa gacgttcaag cagaattggc agcagctggt aaatcattgg ctcaagttga
     7741 agatgttgaa ccagaaccat cacttggtaa cggtggtttg ggacgtctag cttcatgttt
     7801 cgttgattct atgtcaacac ttgctatcaa cggtgaaggt gttggtctga actaccactg
     7861 tggtcttttc cgccaagtat ttaagaaaaa tgagcaagaa gcagagccaa acttctggat
     7921 cgaaaatgat tcttggttga ttccaacaac tatcagctat gatgttccat tcaaaaactt
     7981 cactttgaaa tcaaaattgg accgtcttga tattcttggc tataaaaaag agactaagaa
     8041 ctacctcaat ttgtttgata ttgagtcagt aaactatgac ttgattacag acggtatttc
     8101 atttgacaag actgatatta aagaaaactt gacactcttc ttgtacccag atgattctga
     8161 taagaatggc gaattgctcc gtatctacca acaatatttc atggtatcaa atgcggctca
     8221 acttttgatt gatgaagcaa ttgagcgcgg ttcaaacttg catgacttgg ctgactacgc
     8281 ttatgtgcaa atcaacgata ctcacccatc aatggtgatt cctgagttga tccgtctttt
     8341 gactgaaaag catggtattg aatttgcgga agcagttgcc atcgttaaga atatgactgg
     8401 ttacacaaac cacactatct tggcggaagc gcttgaaaaa tggccacttg agttccttga
     8461 agaagtggtg ccgcacttgg ttgatattat caaagaattg gatgccctta ttcgtgcaga
     8521 aattaaagac ccagcagtcc aaatcattga tgaatcaggt cgtgtacaca tggctcacat
     8581 ggatatccac ttctctaact cagtgaacgg tgtagctgcg cttcacacag aaatcctcaa
     8641 aaactctgaa ttgaaagctt tctacgagct ttatccagaa aaattcaaca ataaaacaaa
     8701 cggtatcact ttccgccgtt ggttggaatt tgccaaccaa gacctcgctg actacatcaa
     8761 agaattgatt ggtgatgagt acttgacaga tgcgactaaa cttgaaaaat tgctggcctt
     8821 tgctgatgac aaagaagttc atgttaagtt ggcggaaatc aaacacaaca acaaattggc
     8881 tctcaaacgt tatttgaaag acaataaggg tatcgagctg gatgaaaatt ctattattga
     8941 tacacaaatc aaacgtttcc acgagtacaa acgccaacaa atgaatgcct tgtatgttat
     9001 ccataaatac cttgaaatca agaatggtaa ccttccgaaa cgtaaaatca ctgttatctt
     9061 cggtggtaaa gcggcacctg cctacatcat tgcacaagac atcattcact tgattctttg
     9121 cttgtcagag ttgatcaaca atgacccaga agtaaataaa tacctcaatg ttcacttggt
     9181 tgaaaactac aatgtgacag ttgctgagaa actcatccct gcaacagata tttcagaaca
     9241 aatctcattg gcttctaaag aagcgtcagg tactggtaac atgaaattca tgttgaacgg
     9301 tgcgttgaca cttggtacaa tggacggtgc caacgttgag attgcagagt tggctggtat
     9361 ggacaacatc tatacatttg gtaaagattc agataccatc atcgacttgt acgataaggc
     9421 tggatatgta tctgcggact actacaatgg cgatgccaat atcaaacgtg cagttgactt
     9481 tatcgttagc gatgaggtaa aagcacttgg taacgaagaa cgtcttggcc gtctgcacca
     9541 tgaattgatt tcgaaagact ggttcatgac cttgattgac ttggctgaat acattgaagt
     9601 aaaagaacaa gtctttgcag attacgaaga tcaagattca tggaacaaga aagttgttca
     9661 caacattgcc aaagctggat tcttctcatc agaccgtacc attgaacagt acaatcaaga
     9721 catctggcac agtaactaat tgaattccaa caattagtag gaaagttgat tgaaccactc
     9781 caaaacttat cagatcaatt cttcctatgt gttgaagaat aaaaactacc agtttttatc
     9841 attattggtg ctctccttag tatataaatg caaagaatgt ctctgcaaaa aagcggagac
//
LOCUS       DATUCG010000094         9690 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.096, whole genome shotgun sequence.
ACCESSION   DATUCG010000094 DATUCG010000000
VERSION     DATUCG010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9690
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.096"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            126..223
                     /gene="ffs"
                     /locus_tag="VCA20_001756"
     ncRNA           126..223
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VCA20_001756"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            550..1092
                     /locus_tag="VCA20_001757"
     CDS             550..1092
                     /locus_tag="VCA20_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835534.1"
                     /translation="MFVFAFPGMGKTTLAKKYSSVVDLEMSHIKYDNSSVQHLSKEER
                     KSTKRPLKDKRYKTIYVDKAYSLHEDGKVVLVALNFLARMLVAMIVRGGVLFHIFIPH
                     PFLKEEYRQRYISRGNNQRFILEVMFIWYIALIPLYMLAKLFPYWITVTHAGETLEDY
                     YKCENFIEFSRKSKNAQTIS"
     gene            1174..2523
                     /locus_tag="VCA20_001758"
     CDS             1174..2523
                     /locus_tag="VCA20_001758"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEP1835535.1"
                     /translation="MTHITFDYSKVLGQFVGAQEVDYMQPQITLADELLRKGTGAGSD
                     YIGWLDLPENYDKEEFARIKEAAAKIQNESEVLVVIGIGGSYLGGKAAIDFLSSSFAN
                     LQTREERKAPQILYAGNSISSSYLADLVDYVADKDFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDKARGAVKVEADANGWETFVVPDDVGGRFSVLTAVGLL
                     PIAAAGADIDALMEGANAARKDYSSDKIAENAAYQYAAVRNILYRKGYVTEILANYEP
                     SLQYFSEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGNRNLFETVVRVDK
                     PRKNILIPEMAEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGGVPNMFVTLPQQDAF
                     TLGYTIYFFELAIAVSGYLNGINPFDQPGVEAYKANMFALLGKPGYEELSAELNARL"
     gene            complement(2728..3357)
                     /gene="eda"
                     /locus_tag="VCA20_001759"
     CDS             complement(2728..3357)
                     /gene="eda"
                     /locus_tag="VCA20_001759"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002381075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEP1835536.1"
                     /translation="MTSLEKQLQSEKLLPLYTVTDLALLPIAEKILLENQLHFIEVTY
                     RSSLASVAIRQLAESNKLIVGAGTVCDLETAKDAIANGAKFIVMPGLNTEVINYCIEQ
                     NVPVYPGAVTPSEIIQAMNLGLKTVKFFPANIYGGLNAIKNLIGPFPNIQFIPTGGIN
                     ENNFVEYTKNNSILAVGGSFILSENLIAKDMGLTASKNLQLLLEKLNKQ"
     gene            3495..4486
                     /locus_tag="VCA20_001760"
                     /pseudo
     CDS             3495..4486
                     /locus_tag="VCA20_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374640.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
     gene            4507..5328
                     /locus_tag="VCA20_001761"
     CDS             4507..5328
                     /locus_tag="VCA20_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEP1835537.1"
                     /translation="MQTLTVMQSLLIALWTAAVMSRWLGGGATLTLRFSPLMTGLIAG
                     IVMGNVGEAMVITAALQLIYMGVFSPGGSMPSEPAVAAAIAIPVALLGDMTPEAAIAV
                     AVPVGLLGSYLYQFRFFLNTFTGRAIDRAVEKMDDKAIARAIIWYPTITSFLLFVPLI
                     FVALYYGAPVIADVISQLQGTPAIHVLEVLGGGLAAIGIATTIYVIGRRDLLVFFFLA
                     YFLSIIFKSLEVTMVTYAVLGAIVAGIYVLLKTEPVVVNSSTRNIDLDDEDDEDF"
     gene            5356..6166
                     /locus_tag="VCA20_001762"
                     /pseudo
     CDS             5356..6166
                     /locus_tag="VCA20_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755486.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
     gene            6183..6686
                     /locus_tag="VCA20_001763"
     CDS             6183..6686
                     /locus_tag="VCA20_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEP1835538.1"
                     /translation="MGQINLARIDERLIHGQVMLTLSQREGVNSIFVVDDVVAKDKFM
                     KDLYKSAGSRTGQKTIVVSVNKAKEYWDEYAFKDYRPILICKTVDVIYELVTHGVPMK
                     ELNLGGIAQKSPSDKQVTKTVYLNENDARKLKELNENYGVENIYFQSTPSAEKTSLKS
                     VLAMFNL"
     gene            6701..7591
                     /locus_tag="VCA20_001764"
     CDS             6701..7591
                     /locus_tag="VCA20_001764"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835539.1"
                     /translation="MESDEYKADLFVRYNHFINKRVTFKQKTAFIRSLMTEFQMIDSE
                     IKVVELREKETRTSLSRNIYIGDVKNADVIIATYYDSPLYHFGPYYILDRIKQQNNTL
                     LFNGLVSSILLLSGVLFTLQITNKILPHGLVNPDWRVLLGIGFVYLLLTFLIGAFAKG
                     IGRRRNAIRNNSTIIYLIDKILQTGKLSPKIAYAFLDNGVTNERGLYGLREFVKEGSK
                     ILYLDSIGSSEELRYIYEASGEFEVEQSNLFARAGTYRIFPARYGKDGYFLTSKELNS
                     AIISIENSIAVDRIIKQSTK"
     gene            7603..8049
                     /locus_tag="VCA20_001765"
     CDS             7603..8049
                     /locus_tag="VCA20_001765"
                     /inference="COORDINATES: protein motif:HMM:NF015567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEP1835540.1"
                     /translation="MFKIIIATHGEMSTGIKDAVETIAGIGEDISTLNLISGKDVNDL
                     GREYLELLESPSYDKGVLIFTDLTSASPYNQALLAINSLEQSKQSDIFVIGGVNLPMV
                     LEAVNHNLLNSDLKDVVDLITSQGKSSIEIWNATHLENADDEDDDF"
     gene            8064..9173
                     /locus_tag="VCA20_001766"
     CDS             8064..9173
                     /locus_tag="VCA20_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003125575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEP1835541.1"
                     /translation="MDYKLQIKPGPQYYSYSEGAIDEIVTLLEEYQVKKVLILHGTIS
                     WEKAKKYFDTILNTNFEFEFEQYSGECSFNEAKRISEIIQSKNIDFVIGVGGGKLSDL
                     TLLATSYTMTPFGLVPTLASNCAPWAALSVMYKDDGMSEGFSEHQKHNAAFLVTDPRL
                     VVDSPIRYFKAGIADTIVKWYESDLVTSQVDIKDYPLVRMGRYAASMCKDDILKYSEK
                     AISDMEKKIPSFEFFVVSEIVIVISGLVGGLAGKYARNTAAHTVHDILSAKLPGLHKY
                     LHGEKVAYGTFYQLALEGKWDEIDKLVPYYRQWDLPLSLTEMGVYPFEDGKLEEISEL
                     MDKKDKIHLLPLEINSEVLGNVYIELEKYLEKYSN"
     gene            9189..>9690
                     /locus_tag="VCA20_001767"
     CDS             9189..>9690
                     /locus_tag="VCA20_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016181959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEP1835542.1"
                     /translation="MKWTFRWYGKDDPITLDKIRQIPNMHGVVGTLVGKLPGDVWELP
                     EIEELKKSVEAKGMVLAGIESVSIHDSIKAGTSERDQYIENYKETVRNLGKAGVGVIC
                     YSFKPIFGWAKTNLEYELPDGSTTLEYDDNVVQHIEPKQMFGLITGQTKGFRLSGWEP
                     ERLDKFY"
BASE COUNT         3118 a         1461 c         2118 g         2993 t
ORIGIN      
        1 ttgtaaaaag gtagatacta agtttaaagc agttatttct ggggacaaaa ggagagaaga
       61 aatttttcat gcgtatggcg tggtcctaat cgactcccac ttgcattcta gtcaaattgt
      121 gttataataa tattcggagc aacattcgtg cgtgaagcgg gtcaggggag gaatccagca
      181 gccctaagcg agttcgagtg tgtgctcttt ttgtgtatag tctaaaaacc ctttgaaatc
      241 aagctttctg agggttttct ttggcttatt tttagaaata ggggcaaaat aggggccgtc
      301 aaatcaaacc acgaacaaaa ttgtccaagc tacaataacc cagcccataa tgaaaagaat
      361 aaaagtcagg cattgtcagg gggggaggcg gtttgaagta tactttggcc tttattgttc
      421 gaattctttt gtcatggtcg cgtaacatgc tgtgtttgaa ttaataacag atggctcagt
      481 ttttcgaatt ttgttaagaa aacttttgga ctgctctgct attctattga aaaagaagga
      541 gggaagtcta tgtttgtatt tgcctttcca ggtatgggga aaacgactct tgctaagaaa
      601 tatagcagtg tggtggactt agaaatgtcc catattaaat atgataatag tagtgtgcag
      661 catttgagta aggaagaacg aaaatcaacg aagcgtcctt taaaagataa gcgctataaa
      721 acaatctatg tggataaagc atatagtctc catgaggatg gaaaagttgt tttagtggca
      781 ctgaattttt tagccagaat gttagttgca atgatagtac gtggtggggt cttgtttcat
      841 attttcattc cacatccgtt tttaaaagag gaatatcgcc aacgctatat ttctagagga
      901 aacaaccaac gctttatcct tgaggttatg tttatttggt acatcgctct gattccgctg
      961 tatatgctag ctaagctatt tccctactgg attactgtaa cacacgctgg agagacccta
     1021 gaagattatt ataaatgtga aaatttcata gaattttcaa gaaaatcaaa aaatgcgcaa
     1081 accatttcat gaaaattaat gaaaattgat aaattccttg aaaaaaccct gataaagcgt
     1141 tatcatatta tggtatacaa atggaggaaa aacatgacac atattacatt tgattattca
     1201 aaagtcttag gccaatttgt tggtgcacaa gaagttgatt acatgcaacc gcaaattact
     1261 cttgctgatg agttgttgcg caagggaact ggtgcgggta gcgactatat tggttggttg
     1321 gatttgccag aaaactatga caaagaagag tttgctcgta ttaaagaagc agcagcgaaa
     1381 atccaaaatg aaagcgaagt cttggttgtg attggtatcg gtggttctta ccttggtggc
     1441 aaagcagcga ttgatttctt gagtagctcg tttgcgaacc tgcaaacgcg tgaagaacgt
     1501 aaagcgccac aaatcttgta cgctggaaat tctatctcat caagctacct tgctgatttg
     1561 gtggattacg tggcagacaa ggatttctct gttaacgtga tttcaaaatc aggtacaaca
     1621 actgaaccag ccattgcttt ccgtgttttt aaagaattgt tggtgaaaaa atacggacaa
     1681 gaagaagcca acaagcgcat ctatgcgaca acagataagg ctcgtggagc agtcaaggtt
     1741 gaggctgatg ccaatggttg ggaaacattt gtcgtgccag atgatgtcgg cggacgtttc
     1801 tcagtcttga cagctgtggg tctcttgcca atcgcagcag ctggtgcaga cattgatgcc
     1861 cttatggaag gtgcaaatgc tgcccgcaaa gactactctt cagataaaat tgctgaaaat
     1921 gctgcttacc aatatgcggc agttcgcaat attctttaca gaaaaggcta tgtgactgaa
     1981 attttggcca actacgaacc gtcccttcaa tactttagcg aatggtggaa acagttggca
     2041 ggtgaatcag aaggaaaaga ccaaaaaggt atttacccaa cttcagccaa cttctcaaca
     2101 gacttgcact ctcttggtca atttatccaa gaaggcaacc gcaacctttt tgaaacagtt
     2161 gtccgtgtgg acaaaccaag aaaaaatatc ctcatccctg aaatggcaga ggatttggat
     2221 ggtcttggct acttgcaagg caaggacgtt gactttgtaa acaaaaaagc aaccgacggc
     2281 gtacttcttg cccacactga cggtggcgtg ccaaatatgt ttgtgacctt gccacaacaa
     2341 gacgcattca cgcttggcta caccatctac ttcttcgaat tggccatcgc tgtatctggt
     2401 tacctcaatg gtatcaatcc atttgaccaa ccaggtgtag aagcctacaa agcaaatatg
     2461 ttcgccctcc ttggcaaacc aggctacgaa gaattgagcg cagaactcaa cgcacgattg
     2521 taatagagta atatttatca acctaattcc taactgacat aggaattagg ttttttattt
     2581 ttgacttaat ctattgatat ttttaaaggg tatcaggaag tctatagtgc atatctaatg
     2641 cattataaag ataaaaagct tcattactct agcaacagta tactctgtgc tagtggcaag
     2701 aaagcttttt ataaacatat tgaatgatta ttgtttattt aatttttcaa gcaataattg
     2761 aagattttta cttgctgtta atcccatatc tttagcaata agattttcgg atagaataaa
     2821 cgaaccaccg acagctaaaa tgctattgtt ttttgtatat tctacaaaat tattttcatt
     2881 aataccgcca gttggaataa actggatatt aggaaatggg cctataaggt tcttaatagc
     2941 atttaaaccg ccatatatat ttgcagggaa aaacttaaca gtttttaagc ctagattcat
     3001 cgcctgaata atttctgagg gggtgacagc tccaggataa acagggacat tttgttcaat
     3061 acagtaattt attacttctg tatttagacc gggcattaca atgaatttag caccatttgc
     3121 aatggcatct ttggctgttt ctaaatcaca tacagtgcct gcacctacaa ttagtttatt
     3181 tgactcagct aattgacgga ttgctacaga agcgagtgag cttctataag tcacttcgat
     3241 aaagtgtagt tgattctcta gcaaaatttt ttctgcaata ggcaataatg ctaaatcggt
     3301 cactgtatac aaagggagta gcttttcgct ttgtaattgt ttttctaatg aggtcatgtg
     3361 acaccatcct tattaatttg ataaatccat tttagaatta ttttttctaa aatgcaagtt
     3421 ttttataaaa gcgcttgcaa aaaaaatatt ttttattata atgtaattat aataaaagaa
     3481 atcggagcaa cgcaatgaac aaaatagcaa tcgtaaattc aagtagtttc ggtaagattt
     3541 ttgttgagca ccttgagaaa ttacgtgaga taggagaagt tgagtttttt aattttgatc
     3601 aacatattcc aggaaaagaa cttgcagaag cattgaaggg ttataattat attattgcaa
     3661 gtgtgacgcc gttttttgat aaggaatttt ttgaaaacaa agacgagctg agactattat
     3721 ctcgtcacgg gatagggtat aacaatgtgg atttagaggc tgctaaggag cataatacct
     3781 tggttacgat tattcctgca cttgttgaaa gggaggctgt tgcggagcaa aatgttacaa
     3841 atttattgaa tttaatgaga agggtttctc aatcaaccaa gcgtgtccta tcggatagat
     3901 gggaggaacg atcacagttt gttggtaaca ccttatatgg aaaaacggta ggaataattg
     3961 gtatagggaa tacaggtagc tgcattgctg agacattacg atatggattt agatgtcgcg
     4021 taattagtta tgatcccaat aaatctgagt tagaaatgca acaatatggt gttgaaaaag
     4081 tagatttaga atatcttttg gaaaattcgg atgttatctg tttagcagcg aacttaactg
     4141 acgaatctta ccacatggta aatggagaaa ctataaaaaa atgaaggtag gagtatatat
     4201 ttcaaacagt gctagaggag ctttagttga tgaaacagca attattgaag gtttagagtc
     4261 tggtcagata gcaggctatg ctacagatgt gctagaagtt gaaccgggta gagcgagtca
     4321 tttgttttta caatttgaca atgttattat gacacctcat acaggagcat acacagcaga
     4381 atgtctggaa gagatggggc ataaatgtgt tcaagatatt gaggatgttg ttcaaggtaa
     4441 aatgcctgta cgggccattc aatcacatag tctatatata aaataacaaa ataaaggaga
     4501 tatattatgc aaactttaac agtgatgcaa agtttattga tcgcgctatg gacagcagct
     4561 gtaatgtcca gatggttggg tggaggagca actctaacgc ttcgattctc tccattgatg
     4621 acgggtctga tagccggaat tgttatgggg aatgttgggg aagcgatggt gattactgct
     4681 gcattacaat taatttatat gggcgttttc tctccgggtg gttccatgcc ctcagaacca
     4741 gcagttgctg cggccattgc gattccagtt gcactgttgg gagatatgac accagaggct
     4801 gctattgcgg ttgcagttcc ggttgggcta ttgggaagtt acttgtatca gtttagattt
     4861 ttcctaaata catttacggg acgcgcaatt gatagagctg tagaaaaaat ggatgataag
     4921 gctatagcaa gagctattat ttggtatccg accattactt ccttcttatt gtttgttccg
     4981 ttgatttttg tagctctata ttatggtgca ccagtaattg cagatgttat ctcacaactt
     5041 caaggaactc cggctattca tgtactagaa gtacttggtg gagggctggc tgcgattggt
     5101 attgcaacaa caatctatgt aattggacgc agagacttac ttgtattctt cttccttgct
     5161 tacttcttaa gtattatctt caaatcattg gaagtgacta tggtaacgta tgctgtttta
     5221 ggagcaattg tagcaggtat ttacgtattg ttgaaaacag agccggtagt ggtaaatagt
     5281 agcactcgca acattgattt agacgatgaa gatgatgaag atttctaatt tagcgtaaac
     5341 gaaggagaaa tgacaatggt tgaaacaaaa acaatacaag aaattactca aaaagatgtc
     5401 acaaaagcgt attcacggtg gcattttgcc aatgaaatcc cacacgcttt tgatagatat
     5461 atggcaccat cattactgta tgcgatgatg ccagttttga aaaaattata tccagatgta
     5521 gaaaagagga aagaagccta taaacgacaa ctgttattct tcaatacaca gctgagctgg
     5581 ggtggtggtg ttattacagg attgatgaca tcaatggaaa gtacacgagc tcaagaagaa
     5641 gctaataacg aacctattaa gatgactgac gatttgatgt ataataccaa agcaggtttg
     5701 atgggagctt tagcaggtat tggtgatgcg attgactcag gaactgtaca atatatcttt
     5761 atcgccattg ggtgctctat tcccgtttgt gtgctttgcg ctttatcagt atgcaatcgg
     5821 tttgttcttt gcaaaacaat cgttcaagat gggacgtgaa gcaaccagtt tgatggcgga
     5881 ctctagtgtt aaaaaagtca ttgaattctt atcagtgcta ggtctattta tgatgggaat
     5941 ccttgcgggt aattatatta aattgtcatc cagtttaaaa tttaccattt ctggcaggga
     6001 gtttgttatt caagagattc tagataagat tattcctgga attttaccgc tagcagctgt
     6061 gttaggaatt tattggttct atagaaaaca tggtcttaat gttatgagag ctatgatgtg
     6121 gttgacaggt atcttggttg tattagctgc attgggaatt ctataatttg taggagaaat
     6181 aaatgggaca aattaattta gcacgaattg acgaacgatt gatacatggt caagttatgc
     6241 tcacattatc acagcgagag ggtgtaaact caatctttgt agttgatgat gtagttgcaa
     6301 aggataagtt tatgaaagat ttgtataaga gtgcagggag ccgtacaggt caaaaaacaa
     6361 tcgttgtatc ggtaaataaa gctaaggaat attgggatga gtatgctttt aaagattaca
     6421 gaccgatttt gatttgtaaa acagtcgatg tgatttatga acttgttact catggtgtgc
     6481 caatgaagga attaaactta ggaggtattg ctcaaaaatc gccatcggat aaacaagtta
     6541 caaagactgt ttatttaaat gaaaatgatg cgagaaaatt aaaagagtta aacgaaaatt
     6601 atggagtgga aaatatttat tttcaatcta cgccgtctgc tgaaaaaaca agtctgaagt
     6661 ctgttttagc aatgtttaac ttataagagt gggataatgt atggaatcag atgaatataa
     6721 agcagatttg ttcgttaggt acaatcattt catcaataag cgtgtgacat tcaagcaaaa
     6781 aacagctttt attcgtagct taatgacaga atttcaaatg atagattcag agataaaagt
     6841 cgttgagttg agggaaaaag agacaaggac atcgttatcc agaaatattt atataggtga
     6901 tgtaaaaaac gcggatgtaa ttatagctac ctattatgac tctccactat atcattttgg
     6961 tccctactat attctggata gaataaagca gcagaataat acattattat ttaatggact
     7021 ggtatcaagt attctgttat tgtcaggtgt gctttttaca ttgcaaataa ccaataaaat
     7081 actcccacat ggacttgtta atcctgactg gagagtactt cttggtatag gatttgttta
     7141 tctacttttg acttttttga ttggtgcttt tgcgaaaggt attggtagaa gaaggaatgc
     7201 gattcggaat aattcaacaa ttatctattt gatagacaag attttacaaa ctggaaaatt
     7261 atccccaaaa atcgcttacg catttctaga taatggagtg acgaatgagc gtggtttgta
     7321 tggattaagg gagttcgtta aggaaggaag taaaattctt tatttagatt ctataggatc
     7381 ttcagaagaa ttacgttata tctatgaggc gagtggggaa tttgaagtgg agcagagtaa
     7441 tctatttgct cgagcaggta cttatagaat tttccctgcc cgatatggca aggatggata
     7501 ttttttgaca agcaaagaat taaattccgc tattatttca atagaaaaca gtattgctgt
     7561 tgatagaatt attaaacaat caaccaaata aaggagtaca gaatgtttaa gataatcatt
     7621 gctacacatg gtgagatgag cacgggaata aaagatgctg ttgaaactat cgctggtatc
     7681 ggtgaagata tttcgacgtt aaacttgatt tctggaaaag atgtcaatga tttagggaga
     7741 gagtacctag aacttttgga gagtcctagt tatgataaag gagtattaat ttttacagat
     7801 ttaacaagtg ctagtcctta taatcaggcg cttctagcga taaatagttt agagcaaagt
     7861 aagcaatcgg atatatttgt tattggtggt gtaaatttac caatggtgtt agaagctgtc
     7921 aatcataact tattaaatag tgatttgaaa gatgtagtag acttaataac ttcacagggg
     7981 aaaagttcga ttgaaatttg gaatgctaca catttggaaa atgctgatga cgaagatgat
     8041 gatttttaat ggagggatat ctcatggatt acaaattaca aattaaacct ggacctcaat
     8101 attattcata ttctgaaggg gctattgatg aaattgttac cttattagag gaatatcagg
     8161 tgaagaaagt tctgatatta catggcacta tctcgtggga aaaggcaaaa aaatattttg
     8221 atactatctt gaatactaat tttgagtttg aatttgagca gtatagtgga gaatgtagct
     8281 ttaacgaagc taagcgaatc tcagagataa tccagtccaa aaatattgat tttgtaattg
     8341 gtgttggagg agggaaatta tcggatttaa ccttactagc cacttcttat acgatgacgc
     8401 ctttcggtct tgtcccaaca ttagcaagta actgtgcacc ttgggcggcg ctgtctgtta
     8461 tgtataaaga tgacggaatg tctgaagggt tttcagagca tcaaaagcac aatgcagcat
     8521 ttttagtcac agatccgcga cttgtagtgg attctccaat tcgctatttc aaagcgggta
     8581 ttgcagatac aattgtgaaa tggtatgaat cagatttggt aacatctcaa gtagatatca
     8641 aagattaccc tttggtaaga atgggaagat atgctgctag tatgtgcaag gatgatattt
     8701 taaaatactc tgaaaaagct atttcagata tggagaaaaa aataccatca tttgaatttt
     8761 tcgttgtctc tgagattgta attgtaattt cgggcttggt tggtggatta gcaggaaaat
     8821 atgcaagaaa tacggctgct cacacggttc atgatatttt gtctgctaaa ttacctggtt
     8881 tacataagta tttacatggt gaaaaagttg cttatggtac cttctatcaa cttgccttag
     8941 aaggtaaatg ggatgaaatt gataaattag ttccgtatta tcgccaatgg gatttacctt
     9001 tatcattaac agaaatgggt gtctatccat ttgaagatgg taagttagaa gaaatttctg
     9061 agttgatgga taaaaaagac aaaattcatt tattgccatt agagattaat tcggaagttt
     9121 tagggaatgt ctatatagaa ttagagaaat atttagaaaa atacagtaat taagaatgga
     9181 gcgtgcttat gaagtggaca tttagatggt atgggaaaga tgatcccata acacttgata
     9241 aaatacgaca aatccctaat atgcatggag tagttggtac attggttggt aaattgccag
     9301 gagatgtttg ggaactacct gaaattgagg aattgaagaa atctgttgaa gcaaaaggta
     9361 tggttttggc tggaatcgag agtgtttcga ttcatgattc cataaaagcg ggaacatctg
     9421 agcgggatca atatatcgaa aactataaag agaccgttag aaatttaggt aaagcagggg
     9481 taggtgttat ttgctatagc tttaagccaa tttttggttg ggctaaaacg aacttggagt
     9541 atgaattacc agatggttcg acaacattag aatatgatga taatgttgtg cagcacattg
     9601 agcctaaaca gatgtttgga ttaatcacag gacagactaa aggttttagg ttatcaggct
     9661 gggaaccgga gcgtttggat aaattttata
//
LOCUS       DATUCG010000095         9602 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.097, whole genome shotgun sequence.
ACCESSION   DATUCG010000095 DATUCG010000000
VERSION     DATUCG010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9602
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.097"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(1..4166)
                     /locus_tag="VCA20_001768"
                     /pseudo
     CDS             complement(1..4166)
                     /locus_tag="VCA20_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071192.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
     gene            complement(4215..4766)
                     /locus_tag="VCA20_001769"
     CDS             complement(4215..4766)
                     /locus_tag="VCA20_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835543.1"
                     /translation="MAWNFDTMKEALSEMEKVDYQEFIKAFLSLELSISDRTILNHVY
                     QDYMDEDDLSLISDELRVKVDGYQDEVQADMTDILEKLYRTGEGSSFIMDLMSSNSLS
                     DTLEQYEVLDSDDYSPLSLETLQAMIQQDLAISSQDYFGDLVHLALQKDLLDQKSHFL
                     QYYVATVMEGIPQERDQRALVLD"
     gene            complement(4750..4941)
                     /locus_tag="VCA20_001770"
     CDS             complement(4750..4941)
                     /locus_tag="VCA20_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEP1835544.1"
                     /translation="MNPKTIYEKDSDQDGLTDAQELALGTNPQSVDTDGDGQADLEEL
                     QSGHSPLVPQKELCDGLEL"
     gene            complement(4942..>9602)
                     /locus_tag="VCA20_001771"
     CDS             complement(4942..>9602)
                     /locus_tag="VCA20_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEP1835545.1"
                     /translation="PISEQTESLALTGQSNGAIAVTVPHDTVTQAVEEAKVEGVSTVE
                     DSPMDLGNTTSATETNQQISKAEEDAQKQVEAINEVTKTYKADKATYESNKARIEQEN
                     KELSQAYEGANQTGKETNTWVDTKVNDLKTRYADADVTVKEQVVSSGNGTSVLDYTNY
                     GKAVETIQSTNEQAVADYLTKKTKADDIVAKNQAIQKENEAGLAKAKADNEAIERRNQ
                     AGQAAVDAENRAGQAAVDQANQEKQQLVSDRAAEIEAITKRNKEKEASARKENEAIDA
                     YNAKEMERYQRDLAEISKGEEGYISEALAQALNLNNGEPQAQHGAITRNPDKIISTGD
                     ALLGGYSRILDSTGFVVYDSFKTGETLSFNYQNLQHARFDGKKISRVTYDITNLVSPA
                     GTDAVKLVVPNDPTEGFIAYRNDGKGDWRTDKIEFRVVAKYFLEDSSQVTFSKEKPGV
                     FTHSSLNHNDIGLEYVKDTSGKFVPINGSTVQVTNEGLARSLGSNRASDLNLPEEWDT
                     TSSRYAYKGAIVSTVTSGNTYTVTFGQGDMPQNVGLSYWFALNTLPVARTVTPYSPKP
                     HVAVELEPVPEPITVTPDVFTPKTFTPEKPVTFTPKPLEEVVQPSLTLTKVILPVKPI
                     PKELPTPPQVPTVHYHAYHLTTTPEIMKEVVNSDQAKLHEKTVAQDSTVINPLTVDAL
                     SPNRAQTTSLIFEDYLPAGYLFDKETTQKENGNYVLSFDETKNFVTLTAKETLLQEVN
                     KDLTKVYQLTAPKLYGSVQNDGATYSNSYKLLLNKGTTNAYTVTSNVVTVRTPGDGET
                     TTLITPDKNNENADGVLINDTVVALGTTNHYRLIWDLDQYKGERSAKETIARGFFFVD
                     DYPEEVLDVVENGTAVTTLDGQNVSGITVKNYASLNEAPKDLKDKLARAKITPTGAFQ
                     VFLPDDNQAFYDQYVQTGTSLALLTKMTVKDSLYGQTKTYTNKAYQVDFGNGYETKEV
                     TNTLVSPEPKKQNLNKDKVDINGKPMLVGTQNHYTLSWDLDQYRGIKSDKSQIAQGFY
                     FVDDYPEEALLPDEAAIQFVTSDGKTVSRITVKSYAQLSEAPKTLQAALSKQKLQPKG
                     AFQVFMPEDPQDFYESYVTKGENITIVTPMTVLETMLNSGKSYVNVAYQVDFGQAYET
                     NTVTNFVPKLTPHKSNTNQEGISIDGKTVLPNTVNYYKIVLDYSQYKDMVVTDDVLAK
                     GFYLVDDYPEEALTLNPDGIQVLDKDGNRVSGISVSTYASLSEAPKVVQDAMAKRQFK
                     PKGAIQVLSSDDPKAFYDTYVKTGQTLVVTLPMTVKNELTKTGGQYENAAYQIDFGLA
                     YVTETVVNNVPKLDPQKDVVIDLSHKDESLDGKEVALHQTFNYRLVGALIPTKRATDL
                     FEYGFEDNYDEKHDEYNGVYRSYLMTDVILKDGSVLKEGTEVTKYTLQQVDTENGLVS
                     ISFDKSFLEYISDDSAFQADVYLQMKRIAAGQVENTYLHTVNGYVISSNTVVTHTPQP
                     EEPSPNQPTPPQPPIESLEPPVPASILPNTGEQESLLGLIGAGILLGTAYGLKKKEEK
                     "
BASE COUNT         2571 a         2096 c         1838 g         3097 t
ORIGIN      
        1 gttcttgtag gtgtatttcc ttggcacgca ggactgttgt tttctctaca tccgtcacat
       61 tctttttctt atccccttcg acaacttgtt ttgtgatggt tggttgattg gaattaagga
      121 gattttcgaa gatttttcga ccactatcat agcgtgaagc agggacacct aaactcctat
      181 ccattgcgtt ggaataggta aaggcaaact tagattggta agtgataacc ccgtcaatgg
      241 gactgatttc aaggactttt gctacctctt ggtctgacag ttcataggct ttccccataa
      301 aggtttcttg ggcaaatttt ccgtaaacag acaaaggaat ccagcgtgaa ccgattcgat
      361 aatcaatgtc tgccaaagta atacgggctg gtttgatagc ttctagaagt cctgcataat
      421 gcggccagtt aaagtcctga ttgtcttgtt tgacaaatag gtccaccact tctaacttgc
      481 taacgacatc acctgacaga aagtcttggc gagaaacata ggtcaattct ccattcaaat
      541 acttctcagg atcaggcata atgaggtcac ctaactcctc aatcaaggtc atctgcgatt
      601 caacctgata gatagacatc atataagcaa aatcaacacc tcgtccgtca gccaagctcg
      661 aatttaaggc atcaagggca gtatgtacct ttttaacctc tttttcagga cgcactagag
      721 ccttctcaaa ggcaagggat ttagtataga taacagactt tccacttgga tctaaacttt
      781 catcttccaa actagcaaga agcgaatact tgtcatcact atcgaaaaga ttgcgattca
      841 ctgcactatt caaataccca tagtgtttga caaagctatc ataggtacga ttgagtttgc
      901 ctaacaagtg gttaaaggtc tccttatcgt agtcataata gcgttgaatg gcaataactt
      961 cttggtaggc attgcgaata tcgaccattc ctttgattcg tgccacttct ttatcagaca
     1021 atggagcttc atagaaaact gtctttttat aatatccctt aaactgacca cgttttgctg
     1081 catcatcggt cacatagaca tctagagcag tgctatcagt cacctctaag ctattaaatc
     1141 tatcaatctg tttttgagaa cgcttggtgt cccatgcttt gaagttgccc tcttcatcga
     1201 catagtaact gatttcttcc gtcttggttc cgactcgaat gcctttatta tctcgatagt
     1261 aaactgtaga accctggtaa ccaaaactat actgaccaag attctctctc atttctgctg
     1321 gtatggaggt atcaatgact tgtttggtca acacatttgg attaatgatg acttcatttc
     1381 tatcaatctt tcttggggct tttacttgat gtagagctgt ttcaacactt gcaatcaagt
     1441 catcactagt ccccttaaca gaaagtgttc cgccattaaa atttctgacc tcgtaggttc
     1501 ctagcacctg gctattgtat tctccatcaa agtaaggatt gagccaaatg cgactatcct
     1561 tttcataacg aatggaacct gaaaaggcta agtcgtctgc tacatatccc ttgtctaagt
     1621 gtttctggaa gaataacata tccgttgtga cactcgttcc tgcaatggcc ttaaaggcag
     1681 aatcaggcag tcgaacccca ccaagaaatt cagttgtctc acgaatatct tgtaagatgt
     1741 tttctgttcg cttatccatg gttcctgttg aagagataat cgctacttgt ccaccatcat
     1801 ggaccaaata caagtgactt tttgacaaag taatcatgaa tcatgtaagg cctatcgtac
     1861 ctgttatccg caattcgtat attggcaaag ggcacatttg aaatcaccaa atcaaaacta
     1921 ttgtcgttaa aagccaccgt ctcaaagccc ttaatttcaa tatgactatt gggatgaagg
     1981 tgtatggcaa tagctcctgt aatagtatct aattccacac catataactc actcttttct
     2041 cttaagtgtt ttggcatagc cgcaaagaaa ttccctgttc ccatggaagg atctaggatt
     2101 ttgccacctg taaagccatc tctttccaac ttatcccaca tctgacggat cagggcaggg
     2161 tctgtataat aggctgtcag agaggactgt ttcatatccg aatactcttt atctgtgact
     2221 aggcttttca gttcttctcg ttccttagaa aattttggat tatagtcatc aaaaaattca
     2281 ttggctagtc caccccagcc tacatacttg gcaaggagtt cttgttcatt tttacttgcc
     2341 tgacggtgtc ccgtttcaag tgttttaacc aaacgaatgg ccgcaatgtt tgtctcaacc
     2401 ttatctctag cagtctttgg ataaaagtcc gtcatatctt ctggaaaata aaagtccatt
     2461 actggaatct ctggaattgt ttcgactact tcctcttcag gaatttgact atctgattct
     2521 tcaagatcag tatcgttatg acctttttct gaaacatctg gttccaatag tccaatactc
     2581 tgaactggct cctcttccag aaatgaaaaa agatttagtt cttggtccgc ttcttctaac
     2641 tctgtttgtg gttctgcctt tggctgatga agtgcctgac tgacttcttc ccaggtcctc
     2701 acataaagaa ctggattttg atcaatgata tccgaaaaat cattgactaa ctcaatccga
     2761 atcaaaccgt tcaatcgagc atcgctgacc gacacgacct caaagtcctg tcccttatag
     2821 gaaaccaatg accctagagg ataagtgtcc aagactctct gaaccagtgg atttcccgca
     2881 aacgcttcat caactacttc tttcgtctca gctttctcca acttcctttt ccaatctcta
     2941 agggttaaat ctggctgatt acctttatct ttttcaaatt gctccgcatc ttctctgctt
     3001 agttcttggt gtaaaaaggc gtaggcaact tgtcgcagct cttcaatgga aagctggcta
     3061 ccaaagaaaa attcaacctt ttcatgactc aatcccattt ggagtagttc tgtcgcaagg
     3121 gctagttgag ttaccgaggc tttgggatag ccacctagta aagctaggtc cgcatttttc
     3181 aaacgaaaac gacctgccgc atccaataaa ataccgactt gatctgattc aaactgagat
     3241 agctcaatcc ctagctgagt caatttttct tgattttcta tcctaatagc tcgtccaatt
     3301 ttttctaatt ccttttcttc gactgtagtc agcgttcgtt tcagctcata gggactacca
     3361 tcagcttcta gataggtaag taactcctcg ccctcaaaga ttggtgtcat ttccatttca
     3421 gtgtcaaaat cacctcgttt aaaacttaag acttttgaac gttcatttcg aatatggtca
     3481 aaatatgtta gaatctcaaa tttacgtcgt aatgacaact cttcataagc tggcagatga
     3541 cttgtttttc ctgtttgaac atctagttga gacaaatcta catccaaact agcaataatt
     3601 agcgacccct cttctttaaa tgatattgct tgatgaacat ttgttttgtg ctcatcaaca
     3661 gtttcttcat tttctagaac agtttcatca actcctgttt caagctcagg ctcgtaagtc
     3721 aatagaaact gctcaatatc tcggctgatt cgatccgcta aattattagc cacacgatac
     3781 acattgacca aattagctcc cctactcttt tgtaagagtg aatctggtga aatagtttgg
     3841 tagtgtccat ttgagtctaa tagttgaaaa cgagtggcca gattgaacaa ggccacttca
     3901 atcaccaagt ctttttcaga cggcgatagt ttgtcgagtt tttgaagacc agtttggccg
     3961 aaaacttgtg tagctgaaac atctgtctca aagtgaagta cctgaacaga tttcttaatt
     4021 tctttcctat tggttaaaaa ctgacgaata agactttccc actcctcatc aaaatgctct
     4081 gcttggtaat ataaaaatgc ctcaagcagg gctctatcac gaggaatggt ggctctcatc
     4141 atttgtagta agacttcttg attcattttc cctcccattt taaaagggct gagagttttt
     4201 ctcagccctt tgcgttaatc taggaccaag gctcgttggt ctctttcttg tggaatacct
     4261 tccattacag ttgccacata gtattgtaag aaatgacttt tctggtctag taaatctttt
     4321 tgcaaggcaa ggtgaaccaa atctccaaaa taatcctgac tggaaatagc caagtcctgt
     4381 tgaatcatgg cctgtaaggt ttcaaggcta agtggtgaat aatcgtcact atccaaaacc
     4441 tcatactgtt ccaaagtgtc tgagagacta tttgaggaca ttaaatccat aataaaacta
     4501 gagccttcac ctgttcgata caacttttcc agaatatcag tcatatctgc ctgtacttca
     4561 tcctgataac catcaacttt aactcgtagc tcatcactaa tcaaggacag gtcatcttca
     4621 tccatataat cttgataaac atgattgagg attgttctat cagaaatact taattccaaa
     4681 gagagaaagg ctttgataaa ctcttggtaa tcgaccttct ccatttcaga caaagcctct
     4741 ttcatggtgt caaagttcca agccatcaca caactccttt tgtgggacta gtggtgaatg
     4801 tccagattgt agctcctcta aatcagcttg accatcacca tctgtgtcaa cagactgcgg
     4861 attggttccc aaagccagtt cctgagcatc tgtcaaacca tcttggtctg aatccttttc
     4921 ataaatagtt tttggattca tctacttctc ctcctttttc ttcagtccat aagccgtacc
     4981 aagtagaata ccagctccaa tcaagcccaa aagggattcc tgttcccctg tatttggcaa
     5041 aatgcttgct ggaacaggtg gttcaagaga ctcaatcggt ggttgaggtg gagtgggttg
     5101 atttggactt ggttcttcag gttgaggtgt atgtgttaca actgtatttg aactgatgac
     5161 atagccattc actgtatgga gataggtatt ctccacctga ccagccgcaa tccgtttcat
     5221 ctgcaggtaa acatctgcct gaaaagctga atcatcagag atatactcta agaaggattt
     5281 atcaaatgaa attgacacta ggccattttc tgtatctacc tgttgcaagg tatatttcgt
     5341 aacctccgtt ccttctttta agacagaacc gtctttaagg atgacatccg tcatcagata
     5401 gctgcgataa acaccattat actcatcatg cttttcatca tagttatctt caaaaccata
     5461 ttcaaataaa tcagtcgcac gcttggttgg aatcaatgct ccaaccaagc gatagttaaa
     5521 ggtttgatgc aaggccactt cttttccatc aaggctctca tctttatgag acaagtcgat
     5581 caccacatct ttttgtgggt ctaatttggg aacattatta accactgttt ccgtgacata
     5641 ggccaagcca aaatcaatct gataggctgc attttcatac tgaccacctg ttttggtcaa
     5701 ctcatttttt accgtcatcg gaagcgtgac aactaaggtt tgaccagtct tcacataggt
     5761 atcataaaag gcttttggat catcgctact aagaacttga atggctcctt taggtttaaa
     5821 ctgacgttta gccatggcat cttgaacaac tttcggagct tctgacaaac tagcgtaggt
     5881 gctgacagag ataccagata cacgattgcc atccttatcc aaaacttgaa tgccatcagg
     5941 atttagggta agggcttctt ctgggtaatc gtctaccagg taaaatccct tggcaagaac
     6001 atcatccgtc acgaccatgt ccttgtattg actgtaatcc aagacaattt tgtaataatt
     6061 gaccgtattc ggaagaacag tctttccatc aattgaaata ccttcttgat tggtattaga
     6121 cttatgtgga gttagtttag ggacaaaatt ggtcaccgtg ttggtttcat aggcttgccc
     6181 aaagtccacc tgataagcca cgttgacata ggacttccct gaattaagca tggtttccaa
     6241 aaccgtcatc ggagtgacaa tggtaatatt ctcccccttg gtcacataag attcataaaa
     6301 gtcttgtggg tcttcaggca tgaaaacttg aaaagctcct ttaggctgaa gtttttgttt
     6361 agaaagggct gcttgtagcg ttttaggagc ttctgataat tgagcatagg acttcacagt
     6421 gattcttgaa actgttttgc catcagatgt gacaaactga atagctgctt catccggcaa
     6481 taaagcttct tctggataat catccacaaa gtaaaaacct tgtgcaatct gagacttgtc
     6541 tgatttaatc cctcggtatt ggtccaagtc ccatgagaga gtatagtgat tttgagttcc
     6601 cactagcatg ggcttcccat taatgtctac tttgtcttta ttgaggtttt gtttcttggg
     6661 ttctggagaa acaagggtgt tggtcacttc cttggtttca tagccatttc caaaatcaac
     6721 ttgataagcc ttgtttgtat aggtctttgt ctgaccgtag agactatctt taaccgtcat
     6781 tttggtcaaa agagctaaag aagttcctgt ttgaacatac tggtcataaa aggcttggtt
     6841 gtcatccggc aaaaagactt gaaaggcacc tgtcggtgta atcttagcac gagctaattt
     6901 atctttaagg tctttagggg cttcatttag ggaagcatag tttttaaccg ttattcctga
     6961 tacattctga ccgtcaaggg ttgtaacagc cgtgccattt tccaccacat caagcacttc
     7021 ctctgggtaa tcgtccacaa agaagaagcc tcgtgcaatt gtctctttag cagaacgctc
     7081 tcccttatac tggtccaaat cccaaatcaa tcggtagtgg ttggttgtgc caagggctac
     7141 gaccgtgtca ttaatgagga cgccatccgc attttcattg tttttatctg gtgtaatgag
     7201 tgtggttgtc tccccatcac ctggtgtacg aaccgttaca acatttgaag tgactgtgta
     7261 agcattggtt gtgcccttgt tcaaaaggag tttgtaacta ttggaatagg ttgccccatc
     7321 attttgaaca gaaccataga gttttggagc agtcagttga taaaccttgg ttaaatcttt
     7381 attaacctcc tgcaacaagg tttcctttgc agttaaggtc acaaaattct tagtctcatc
     7441 aaagctaagg acatagtttc cattctcttt ttgtgttgtt tccttatcaa ataggtaacc
     7501 agcaggcaag tagtcctcaa aaatgagact agtcgtttgg gcacgattgg gcgataaggc
     7561 atcaactgtt aagggattaa tcaccgttga atcttgtgcg acagttttct catgaagttt
     7621 agcttggtca ctattgacca cttctttcat aatctctgga gttgtcgtca aatgatacgc
     7681 atgataatgg acagtcggaa cttgtggtgg cgttggaagt tcttttggaa taggtttaac
     7741 aggtaagatt accttggtca aagttagact aggctgcacc acttcttcca aaggctttgg
     7801 cgtgaaggtt acaggctttt caggtgtaaa ggtttttgga gtaaagacat ctggtgttac
     7861 cgtaataggt tctggaacgg gttcaagttc caccgctaca tgaggtttgg gactataagg
     7921 tgtcactgta cgtgccactg gtagggtatt taaggcgaac cagtatgaca agcctacatt
     7981 ctgtggcata tcgccttgcc caaaggtgac tgtataggta ttgcctgatg taaccgttga
     8041 gacaatagca cctttataag cataacgact tgatgtggta tcccattctt caggtaaatt
     8101 caaatcactt gcacggttgg aacccaaaga acgtgctaga ccttcattag tcacttgaac
     8161 ggttgaacca ttaatcggca caaatttccc agaagtatct ttgacatatt ctagaccaat
     8221 gtcattatga ttgagggaag aatgcgtgaa gacacctggt ttttctttgg agaaggtaac
     8281 ttgtgaactg tcttccaaga aatacttggc aactacacga aactctatct tgtctgttcg
     8341 ccaatcaccc tttccgtcat ttcgataggc aatgaagccc tcggtaggat catttggtac
     8401 aaccaatttc acggcatcgg ttccagctgg tgatacaagg ttggtaatat cgtaagtcac
     8461 tcgactaatc tttttaccat caaatcgtgc atgttgaaga ttttgatagt taaaactaag
     8521 agtctcacct gttttgaagc tatcatagac aacgaatcct gttgaatcca atattcttga
     8581 gtagccaccc agcaaagcat cgccagttga aataatctta tcaggatttc gggtaatggc
     8641 cccatgttgt gcttgtggct caccgttatt gagattgagg gcttgagcaa gggcttcaga
     8701 aatataacct tcttcacctt tcgaaatctc ggccaagtca cgttgatagc gttccatttc
     8761 tttggcatta taggcatcaa tcgcttcatt ctcttttctg gctgaggctt ctttttcttt
     8821 attacgtttg gtaatagctt caatctcggc tgcacgatct gaaactaatt gttgtttctc
     8881 ctgattagct tgatctactg cggcttgacc tgcacggttt tcagcatcaa cagctgcttg
     8941 accagcctga ttacgccttt caatggcttc attatctgcc tttgccttag caagtccggc
     9001 ttcattttct ttttgaatgg cctgattttt cgcaacaata tcatctgcct ttgttttctt
     9061 tgttagataa tccgctacag cttgttcgtt agttgattga atggtttcaa ccgccttgcc
     9121 atagtttgta tagtctagta cagatgtccc atttcctgat gaaactactt gttctttcac
     9181 tgtcacatca gcatctgcat accgagtttt taggtcattg actttagtat caacccaagt
     9241 atttgtctct ttaccagttt ggttggcccc ctcgtaggcc tgtgacagct ccttattttc
     9301 ctgttcgatg cgagctttat tcgattcgta tgtcgcttta tcagctttgt aggttttagt
     9361 gacttcatta atagcctcaa cctgtttttg ggcatcttct tctgcttttg aaatttgttg
     9421 gttggtctct gtcgcagaag ttgtatttcc taaatccatt ggactatctt caaccgtaga
     9481 aacaccttca acctttgctt cttcaactgc ttgtgttacc gtatcatgtg gtacagtgac
     9541 agcaatcgct ccattagatt gtccagttaa cgctaaactt tcagtttgtt cagatatagg
     9601 tt
//
LOCUS       DATUCG010000096         9352 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.098, whole genome shotgun sequence.
ACCESSION   DATUCG010000096 DATUCG010000000
VERSION     DATUCG010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9352
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.098"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(251..964)
                     /gene="deoD"
                     /locus_tag="VCA20_001772"
     CDS             complement(251..964)
                     /gene="deoD"
                     /locus_tag="VCA20_001772"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEP1835546.1"
                     /translation="MSIHISAKPGEIADKILLPGDPLRAKFIAENFLEDAVCFNEVRN
                     MFGYTGTYKGHRVSVMGTGMGMPSISIYAHELINEYGVKKLIRVGTAGSLNEDVHVRE
                     LVLAQAAATNSRMINIDWPEYDLPQIADFDLLDKAYHIAKELNMTTHVGNVLSSDSFY
                     SPKLFSRNLELGQLGVKAVEMEAAALYYLGAKFGVQTLAIMTISDSLVNPEENTTAEE
                     RQNTFTDMMKVGLETLIAE"
     gene            complement(997..1806)
                     /locus_tag="VCA20_001773"
     CDS             complement(997..1806)
                     /locus_tag="VCA20_001773"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEP1835547.1"
                     /translation="MSLLAKINETKTFLEEKGLVKPEFGLILGSGLGELAQEVENAIV
                     IDYADIPNWGKSTVVGHAGKLVYGDLAGRKVLALQGRFHFYEGNPMEVVTFPVRVMKA
                     LGCEGVIVTNAAGGIGYGPGTLMAITDHINMTGQNPLIGENLEEFGPRFPDMSNAYTK
                     EYREKAHAIAEKLGIKLDDGVYLGVTGPTYETPAEILAFKTMGAHAVGMSTVPEVIVA
                     AHSGMKVLGISAITNFAAGFQSELNHEEVVEVTEQIKGNFKGLVKAILAEL"
     gene            complement(1818..2174)
                     /locus_tag="VCA20_001774"
     CDS             complement(1818..2174)
                     /locus_tag="VCA20_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family transcriptional regulator"
                     /protein_id="HEP1835548.1"
                     /translation="MEKLTVYYNPDCSKCKKLRILLSSQDMEVKWVNYLETPLRGAEL
                     TALLEKMGSQPSAVTRLKEEERLALSEEEIFERLVKEPALLNRPIIEREQTAFLCRPL
                     EIIKEKMPEYDWSDYL"
     gene            complement(2174..3385)
                     /locus_tag="VCA20_001775"
     CDS             complement(2174..3385)
                     /locus_tag="VCA20_001775"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEP1835549.1"
                     /translation="MPKFKRVHLVVMDSVGIGAAPDSDKFFNAGVADTESDTLGHISE
                     KAGLEVPNMTKIGLGNIPRDTALATVPAESHPTGYVTKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWDGFPEEILTKIEEFSGRKIIREANKPYSGTAVIDDFGPRQMETGELIV
                     YTSADPVLQIAAHEDIIPLDELYRICEYARSITLERPALLGRIIARPYVGEPGNFSRT
                     ANRHDYAVSPFEATVLNKLADAGVPTYSVGKISDIFNGSGITNDMGHTKSNMHGVDVL
                     IDTLKLSDFEEGFSFTNLVDFDAVYGHRRNIEGYRDCLQEFDARIPEIIDNMREDDLL
                     LITADHGNDPSYAGTDHTREYVPLLAYSKAFKGSGVLPVGHFADISATVAENFGVDTA
                     MIGESFLSQLV"
     gene            complement(3714..4388)
                     /gene="rpiA"
                     /locus_tag="VCA20_001776"
     CDS             complement(3714..4388)
                     /gene="rpiA"
                     /locus_tag="VCA20_001776"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEP1835550.1"
                     /translation="MTNLKEQVGIKAAEFVTDGMIVGLGTGSTAYYFVQEIGRRVAEE
                     GLHITGVTTSHATAEHAASLGIPLKNIDEVEYVDLTVDGADEVDGDFNGIKGGGAALL
                     MEKVVAVNSKDCIWIVDESKMVQTLGAFKLPVEVVQYGAKNLFRLFEQKGYRPSFRMR
                     NGEKHITDMQNFIIDLDLRRIEDTYALAEELDRTVGVVEHGLFIGLISKVIVGTPEGP
                     NIIEKK"
     gene            4502..4723
                     /locus_tag="VCA20_001777"
     CDS             4502..4723
                     /locus_tag="VCA20_001777"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835551.1"
                     /translation="MLKLFFNHKTWERGVNIMFYLVKIINKLFYFLHNLNDNFFFYWI
                     FDILNFSIFWNLKSANIFYLINGIKSFLP"
     gene            complement(4736..5044)
                     /locus_tag="VCA20_001778"
                     /pseudo
     CDS             complement(4736..5044)
                     /locus_tag="VCA20_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004183149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
     gene            5236..6609
                     /gene="mnmE"
                     /locus_tag="VCA20_001779"
     CDS             5236..6609
                     /gene="mnmE"
                     /locus_tag="VCA20_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002906470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEP1835552.1"
                     /translation="MITKEFDTITAISTPLGEGAIGIVRLSGTDAFAIANKVFKGKDL
                     ATVPSHSLNYGHIIDPTTGQVLDEVMIGAMRSPKTFTREDVIEINTHGGIAVTNEILH
                     LLIRQGARMAEPGEFTKRAFLNGRVDLTQAEAVMDVIRAKTDKAMHNAVRQLDGSLSQ
                     LINDTRQEILNTLAQVEVNIDYPEYDDVEEATTELVREKTLQFQALLENLLRTARRGK
                     ILREGIATAIIGRPNVGKSSLLNNLLREEKAIVTDIAGTTRDVIEEYVNIKGVPLKLI
                     DTAGIRETDDIVEKIGVERSKKALEEADLILLVLNASEPLTEQDRNLLAISDMANRIV
                     LLNKTDLEEQIEADQLPEDVIRISVLKNQNIDLIEEKINQLFFENAGLVEQDATYLSN
                     SRHISLIEQAVQSLQAVNDGLEMGMPVDLLQVDLTRCWQILGEITGDAAPDELITQLF
                     SQFCLGK"
     gene            complement(6970..8379)
                     /locus_tag="VCA20_001780"
     CDS             complement(6970..8379)
                     /locus_tag="VCA20_001780"
                     /inference="COORDINATES: protein motif:HMM:NF012744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEP1835553.1"
                     /translation="MTVYTVRRFLTNQVFGYETTQSYRHKTLSKYRIPNKFLVTTPFF
                     REQMEQRLWSMGFEPENVVILPSTYSDMDPKTFTYSFESFEQSLEVGTNLLNATQFHR
                     TYKVIDGYLDVNMNHLGKVVRILRRNGNLKVISQTFCTEGPYFTSYHDEVSSFEYYNL
                     DGSVAISGYYQNLKVSTAAVYILNGEEIREEDLVIRYLEEYGLRQDVLLNDQLQFKFP
                     KLMHYAETNGFIYREILHYNHFHMLEVSSKYKTIIPEKIITASPYLNQRLLDEGHDAT
                     FIHPVGVTVAQNYPTGLSSNKVLLVGNFHKLKRIEMALQAFQQVPELELHVYGGTTPE
                     ITEFKNSYQIPDNVVFEGSVDSSFIPRSTYAAYLSCSKTEMYANAMVECLGVGLIPIL
                     SKVDYGHNQVLEKLDYGTGFDTVEELIEVLRKFVAWPTDYRKQISEKVLNIAQEFSHE
                     EAESALLNWLKKVGSRDVE"
     gene            complement(8537..>9352)
                     /locus_tag="VCA20_001781"
     CDS             complement(8537..>9352)
                     /locus_tag="VCA20_001781"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835554.1"
                     /translation="QGLVSEGTTTVTYIYQVVPNKTETTSEAVTGTVITRYVDIDTGE
                     EIVSGSTIVDNGIVANTVTTTERNAAGEIVYQTVETIPTGLTYDTTSDKTVKNAEIAL
                     IQIPVTDIALADGPAVGTSVVNNGDGTFTISYTMISSASIYNTDLTTLGEAEVLNRIE
                     KELTRQATERFPGITWSFDSFIDYFETPIPIDGTPYSYVGNKAYTVHATYNISGRTTS
                     YAFVSVDTSEVGAVEEGTTIITYSYRKVPLTFETVAFPLPVESMTVATLPRDW"
BASE COUNT         2672 a         2176 c         1770 g         2734 t
ORIGIN      
        1 taccctattt ataaaaaaaa gacaagccaa cttccgtaca aatagaaatt agttcgtctt
       61 ttaaacgagg ctgggcaaca agcctaggac cacttctcag agttcgtttc aacatctcag
      121 cgcagtggtt gattggcaga tttgttcgtg ttttgcactc caaatctggc ctaatcaact
      181 gtgcgggggt gggaagacga actctttttg aactagtcga gttctttccc actcccagaa
      241 ttattcttaa ttattctgca atcagagttt ccaaaccaac tttcatcata tccgtgaaag
      301 tattttgacg ctcttctgct gtggtgtttt cctctggatt aaccaagcta tctgaaatcg
      361 tcataatggc aagtgtttga acgccaaact tagcacctag gtaataaagt gcagctgctt
      421 ccatttctac agccttgaca cctagttgac ctaattctaa gttgcgacta aacaattttg
      481 gagaatagaa actatctgat gataaaacat tgccaacatg cgttgtcatg ttgagttctt
      541 tggcgatatg ataagcctta tctaaaagat caaaatcagc gatttgtggc aaatcgtact
      601 ctggccagtc aatattgatc atacgagagt tggtcgcagc tgcctgagcc aagaccaatt
      661 cacggacatg gacatcctca ttcaaagaac ctgctgttcc cacacgaatt agttttttca
      721 caccatattc gttaatcaat tcatgggcat agatagagat ggatggcatt cccatacctg
      781 ttcccatgac agagacacga tgacccttgt aggtacctgt gtagccgaac atattgcgaa
      841 cttcgttgaa gcaaactgca tcttcaagga agttttcagc gataaattta gcacgaagcg
      901 gatcaccagg aagcaaaatc ttatcagcga tttcgcctgg ttttgcggaa atatggatag
      961 acatgtaatg tacctctttg ttctattttc gaatgcttat aactcagcca agatagcctt
     1021 caccaagccc ttgaagttgc ccttgatttg ctcggttact tctacaacct cttcgtgatt
     1081 gagttcagat tggaaaccag ccgcaaagtt tgtgatagca gaaataccca agactttcat
     1141 gcctgagtga gctgccacga taacttctgg tactgttgac ataccgactg cgtgggcacc
     1201 catagtcttg aaggccaaga tttcagcagg tgtttcatag gtagggcctg tcacaccgag
     1261 atagacacca tcgtccaact tgatacccaa tttttccgca atggcatggg ctttttcacg
     1321 gtattcttta gtgtaggcat tagacatatc tgggaaacgt ggaccaaatt cttccaagtt
     1381 ttcgccaatc aatggatttt gaccagtcat gttgatgtgg tcggtaatgg ccataagggt
     1441 acctggaccg tagccgatac cacctgcagc atttgttaca atcacgcctt cacaaccaag
     1501 agctttcatg acacggactg ggaaagtcac aacttccatt ggattgcctt cgtaaaaatg
     1561 gaaacgacct tgaagagcta aaaccttacg tcctgccaaa tcaccgtaaa ccaacttacc
     1621 agcatgacca acaactgttg atttacccca gtttggaata tctgcatagt cgatgacaat
     1681 ggcattttca acttcttggg ccaattcgcc caatcctgat cccaaaatca aaccgaattc
     1741 aggttttacc aaaccttttt cttccaagaa cgtttttgtt tcattgattt ttgcaagtaa
     1801 actcatattt tctcctttta taaataatcc gaccagtcat attctggcat tttttcttta
     1861 atgatttcaa gcggacgaca gaggaaggct gtctgctctc gctcgataat ggggcgattg
     1921 agcaaggcag gctctttaac taaacgctca aagatttctt cttctgacaa ggctagtcgt
     1981 tcttcttcct tcaatcgagt aactgctgaa ggttgactgc ccattttttc caagagggct
     2041 gtcaattcag ctccccttaa tggcgtttcc aaataattaa cccacttgac ttccatatcc
     2101 tgactggaca gcaatattct taatttttta cacttgctac agtcaggatt gtaatagaca
     2161 gttagcttct ccattagacc aactgtgata agaagctttc gccaatcatt gctgtgtcca
     2221 ctccgaagtt ttccgcaacg gttgctgaaa tgtccgcaaa atggcctact ggcaagacac
     2281 cgctaccttt gaaagccttg ctgtatgcta agagtggcac atattcgcgt gtgtgatctg
     2341 ttccagcata ggatggatca ttgccatggt ctgcagtaat caagagcagg tcatcttcac
     2401 gcatattgtc aataatctct ggaatacgtg catcaaactc ttgcaaacaa tcgcggtagc
     2461 cttcgatatt gcgacggtga ccatacacag catcaaagtc aactaagttt gtgaaggaga
     2521 aaccttcctc gaagtctgat aacttcagag tatcaatcaa cacatcgaca ccgtgcatgt
     2581 tggacttggt gtgacccata tcatttgtaa tacctgaacc attgaaaata tcactgattt
     2641 taccaactga gtaagttggt acaccagcat ctgccagttt gttgagtact gttgcttcaa
     2701 aaggtgatac tgcatagtcg tgacggttgg cagttcgtga gaaattgcct ggctcaccaa
     2761 cgtatggacg ggcgatgata cggcctagaa gagctgggcg ttcaagggta atggaacgag
     2821 cgtattcaca gatacgatac aactcatcaa gtggaataat atcttcgtgg gctgcaatct
     2881 gaagcacagg gtctgcggag gtatagacga ttaactcccc agtttccatt tggcgagggc
     2941 caaagtcatc aatgacagct gtacctgaat acggcttgtt tgcttcgcgg atgattttac
     3001 gacctgagaa ttcttcaatt tttgtcaaaa tctcttcagg gaaaccatcc cagaatgtat
     3061 caaatggctc agtaatattg agacccataa tttcccagtg tccagtcatg gtatccttac
     3121 caagcgatac ttcttccaac ttggtcacgt aaccagttgg atggctttca gcaggaactg
     3181 tcgcaagcgc tgtatctcga gggatatttc caagaccaat cttggtcata ttaggcacct
     3241 caagcccagc tttttcagaa atgtggccga gagtgtcaga ttctgtatct gctacacccg
     3301 cattgaaaaa tttatctgag tctggtgctg cgccgattcc gacagagtcc ataactacca
     3361 agtgaacacg tttaaattta ggcataattg tctcctttat ttgatatagt tagattcaac
     3421 caaaatcagc gtccaaccaa gttgctagac tggaaattat catcaccaca cctaaacatt
     3481 ttaatggggt gcaatactta tacagtcaaa gctctcggca tcaaacttcc taactatcaa
     3541 aactagatac tgttattcgc ttcaccgcct gctgttcagc tatcgctttt tcagtaccta
     3601 tcatctgact gctacgaggt aaggggatag atttttgaag agtctaaacg ttcgtgtttt
     3661 cattatacca taaaaacgcc tctcattgta ccaaaatgag aagcgttttc aatttatttc
     3721 ttttcgatga tgttcggtcc ttctggtgta ccaacaatta ccttggaaat caatccaata
     3781 aagagaccgt gttctacaac accgacggtc cgatccaatt cttccgccaa tgcatatgtg
     3841 tcttctattc tgcgcaaatc aaggtcaatg ataaagttct gcatatccgt gatatgtttt
     3901 tctccattac gcatacgaaa acttggacga tacccttttt gctcaaacag tcggaaaaga
     3961 tttttcgcac cgtactgtac aacttccacc ggcaacttga aggctcctag ggtttgaacc
     4021 atcttagact catccacaat ccagatgcag tccttactat tgactgctac cactttctcc
     4081 atgagaagcg ctgcaccacc gcctttaatc ccattgaagt ccccatcaac ctcatctgct
     4141 ccatccaccg tcaagtccac atattcgact tcgtcgatat tttttagggg aatcccaagg
     4201 gacgcagcat gttctgccgt ggcatgagag gtcgttacgc ctgttatatg tagcccttct
     4261 tctgcaaccc ggcggccaat ttcctgcacg aaatagtagg cagtcgaacc agttcccagc
     4321 ccaacaatca tcccgtccgt cacaaactcc gccgccttaa tcccaacctg ctcttttagg
     4381 tttgtcatct ttgtctcctt tgaagtcatt catgaaataa ttataccaca gctaagacag
     4441 cagttcaaaa taacaactca aacttcagac agcaaagact acaatatgtg catagtgact
     4501 aatgctgaaa ttatttttca atcataagac ttgggaaaga ggagtcaata tcatgtttta
     4561 tctggtcaag attatcaaca aactctttta ctttttgcat aatttgaatg acaatttctt
     4621 tttctactgg atttttgata ttctcaattt cagtatcttc tggaatttga agagtgctaa
     4681 tatcttctat ctgataaatg gcattaaatc ctttttacca taatattctc ctacattaca
     4741 atttacgtaa ttcttcttta acctcatcct tgcttaccac ctcattaaag aacggcaatt
     4801 cgacaatacg atagcggtca tcatcaacca gtgttctcgc ctcgtctcgc tcggaaatct
     4861 gatgcaagaa ctcttgtttc cagccttcct tgctattttt gaaagtatta tcagaatcca
     4921 agaactggct ccctttcggt tcaatgaaaa tctgccaaga aacatttcct gtatttttgt
     4981 cgttggcaaa catgagaaag tctggttcaa aggcttgccc cttatcaaaa ctataaattt
     5041 ttatgtttct attataacat agtcaaccta aatgaatagc aacacttttt gtatttctat
     5101 caaattatct tctcaaatta attactatat ttattgacct ctcaatacat ttctgcactc
     5161 ctcatccctc atttgtgctt ttttcatcat tccatgatac actaatagat aagacgattt
     5221 tagaaagaaa gatatatgat aactaaggaa tttgacacaa ttaccgctat atccactcct
     5281 ctcggtgagg gggccattgg gattgtccgc ctgtctggga cggacgcttt tgccattgcc
     5341 aacaaggttt tcaagggcaa ggacttggcg actgtaccca gccacagcct caactacggc
     5401 cacattatcg atcctacaac ggggcaagtg ctggacgaag tcatgattgg agccatgcgt
     5461 tctccaaaga ccttcacacg cgaagatgtc attgagatta acacccacgg ggggattgcc
     5521 gttaccaacg aaattctcca cctcctgatt cgccaagggg ctcgaatggc tgagccaggc
     5581 gagtttacca agcgagcctt cctcaacggt cgcgtagatt tgacccaggc cgaggccgtc
     5641 atggatgtca tccgtgccaa gactgacaag gccatgcaca atgccgtccg ccagcttgat
     5701 ggctccctct cccagctcat caacgacacc cgccaggaga ttctcaacac gttggcacag
     5761 gtcgaggtca acatcgacta ccctgagtat gacgacgtcg aggaggcgac aacagagctg
     5821 gtccgcgaga aaaccctcca gttccaagcg cttttggaaa atctcctccg tactgcccgc
     5881 cgtggaaaaa ttctgcgtga aggcatcgca actgccatta tcggtcgtcc caatgtggga
     5941 aaatccagcc tactcaataa cctcctccgc gaggaaaaag ccatcgttac agatatcgca
     6001 ggaacaaccc gcgacgttat cgaagaatac gtcaacatca aaggcgtccc cctcaaacta
     6061 attgataccg caggtatccg tgaaacagat gacatcgttg aaaaaatcgg cgttgaacgg
     6121 tccaaaaaag cccttgagga ggccgacctg atcctgctgg tcctcaacgc atccgagcca
     6181 ctgaccgagc aagatcgcaa cctcctagcc atttcagaca tggccaaccg cattgtcctg
     6241 ctcaacaaga ccgacctaga agaacagatc gaggcagacc aactgcctga ggatgtcatc
     6301 cgcatttccg tcttgaaaaa ccagaacatc gacctgattg aagaaaagat caaccaactc
     6361 ttctttgaaa atgcaggttt ggtcgaacaa gatgccacct atctctccaa ttcccgccac
     6421 atttccctca ttgaacaagc cgtccaaagc ctgcaagctg tcaatgacgg attggaaatg
     6481 ggtatgccag ttgacttgtt acaggttgac ctgacccgct gttggcagat tttgggagaa
     6541 atcacaggag atgcagctcc agacgaactc atcacccaac tctttagcca attctgtctt
     6601 ggaaaataat gtaagtactc tcgaccgtca tttaatctca aataaaaaag cgaacaaatg
     6661 gtaatctgat aatcagaatt tccttttgtt cgctttttta ttttatacat tacaattatt
     6721 acatagaagt agatgaaata gaccaattgt tcatttaggt attccctgct tgtgactaca
     6781 taactgccaa tgcagcctta aactgaaagg gtgtttcacc aaaaatgaaa tttcttaacg
     6841 gcattcctca tttatcatct atttttcatc acagttcctt tcttagaatt aggctagcaa
     6901 aaatcctata ttcaagaaaa gaacacatcg gaccgttaga gcctagatct tctcaaaaag
     6961 aaagagtctt cactctacat ctctagagcc gacttttttt agccagttga gcagggcact
     7021 ctcagcctct tcatgagaaa actcttgagc gatgtttagt accttttctg agatttgctt
     7081 ccgataatct gttggccatg cgacaaactt ccgcaacacc tctatcagct cctcgaccgt
     7141 atcaaaacct gtaccgtaat caagtttttc caagacttgg ttatgcccat aatcaacttt
     7201 tgataggatt ggaataagtc ctactcctag acactctacc atggcattgg catacatttc
     7261 tgtcttagaa caggataaat aggcagcata ggtactacga ggaataaagc ttgaatctac
     7321 agatccttca aatactacat tatcagggat ttgataactg ttcttaaatt ctgtaatctc
     7381 aggtgtagtc ccaccataca catgtaattc caactcaggt acctgctgaa aagcctgtaa
     7441 cgccatttca attcgcttca gtttatgaaa attacctact agcaatacct tatttgaact
     7501 taatcctgtc ggataatttt gggcaactgt aactcctaca ggatgaatga aggtcgcatc
     7561 gtgtccctca tcaagtaatc gttggttcag gtacggacta gctgtaataa ttttttctgg
     7621 aataatcgtt ttgtatttag aactaacttc cagcatatgg aaatgattgt aatgtaaaat
     7681 ctctctgtat ataaagccat tcgtctcagc atagtgcatc aactttggaa atttaaattg
     7741 aagctggtca ttcagaagga catcttgtct cagcccatat tcttctaaat agcgaataac
     7801 taagtcttct tctcttatct cttcaccgtt taatatatag acagctgctg tactaacttt
     7861 caaattttga taatagcccg atatggccac cgaaccatct aaattataat attcaaaaga
     7921 agaaacttca tcatgataac tagtgaaata cggtccttcc gtacaaaatg tttgagagat
     7981 tacttttaga ttcccattgc gacgcaagat ccgtacaacc ttacctaaat ggttcatatt
     8041 tacatcaagg taaccatcaa tcaccttata ggtgcgatga aactgtgttg catttagtaa
     8101 gttcgtccct acttccaacg actgttcgaa agattcgaaa gagtatgtga aggtcttggg
     8161 atccatatct gaatatgtac taggtaaaat cacgacattt tctggttcaa accccataga
     8221 ccaaagccgt tgttccattt gctcacgaaa gaaaggggtt gtgacaagga atttattcgg
     8281 aatcctatat ttacttaatg tcttatgacg ataagattgg gttgtttcat acccaaatac
     8341 ttgattggtg agaaagcgtc ttacggtata tactgtcatc attcctccta tttattaata
     8401 tttctccact acttctagga taagtagtta atactgttta tctactataa gaaaagcgaa
     8461 caaagcatgt ttctgaagca cagaagacac tcgtgttcgc tttactattt ctaatcagat
     8521 ttttcctaca gttctatcac caatctcgtg gcagggtagc aactgtcatg gattctacag
     8581 gtaatgggaa agcaactgtt tcaaatgtta gaggtacttt acgataagag taggtgataa
     8641 tagtcgttcc ttcctctacc gcccctactt cagaagtatc aacgcttaca aacgcatagc
     8701 tggttgtacg accggagata ttataagtag catgtaccgt gtacgcctta ttgccaacat
     8761 aagaataggg tgtaccatca ataggaatag gagtttcaaa gtagtctatg aaagaatcaa
     8821 agctccaagt gatacctggg aacctttcgg tagcttgtct agtcaattcc ttttcgattc
     8881 tattaagaac ttcagcctca ccaagtgtag ttaaatccgt attataaata cttgcagaag
     8941 aaatcattgt gtatgaaatt gtaaaagtac catctccatt attgaccact gaggtaccaa
     9001 cagcaggacc atcagccaat gcaatatcag tgactgggat ttgaatcaag gcaatttctg
     9061 catttttcac tgttttatcg ctcgtggtat cataggtcag tccagtagga attgtttcga
     9121 ctgtctgata aacgatttct ccagccgcat tgcgctcggt cgtagtgacg gtatttgcta
     9181 cgataccatt atccacaatc gtagagccag acacaatttc ttcaccggta tctatatcga
     9241 cataacgagt gatgactgta ccagttaccg cttcagatgt ggtttcagtt ttatttggaa
     9301 ctacttgata gatataggtg acggtcgttg taccttcact aacaaggcct tg
//
LOCUS       DATUCG010000097         9335 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.099, whole genome shotgun sequence.
ACCESSION   DATUCG010000097 DATUCG010000000
VERSION     DATUCG010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9335
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.099"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(57..1040)
                     /locus_tag="VCA20_001782"
     CDS             complement(57..1040)
                     /locus_tag="VCA20_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEP1835555.1"
                     /translation="MSKDIRVLLYYKYVPIENAEAYAAEHLAFCKSIGLKGRILIADE
                     GINGTVSGDYETTQKYMDYVHANPLFSDLWFKIDEENEQAFKKMFVRYKKEIVHLGLE
                     DNDFDNDIDPLVTTGAYLSPKEFKEALLDEDTVVLDTRNDYEYDLGHFRDAIRPDIRN
                     FRELPQWVRDNKEKFMDKRVVVYCTGGVRCEKFSGWMVREGYKDVGQLHGGIATYGKD
                     PEVRGELWDGKMYVFDERIAVDVNHVNPVVVGKDWFDGTPCERYVNCGNPFCNRRILT
                     SEENEHKYVRGCSAECRAHERNRYISENGLTRQEWAERLEVIGETLAPANA"
     gene            complement(1376..2296)
                     /locus_tag="VCA20_001783"
     CDS             complement(1376..2296)
                     /locus_tag="VCA20_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835556.1"
                     /translation="MVWTEEKLGPIIHSILNPDEEVLSLYQSKTTKSIYGCILWNNYY
                     YKVFRVSNHPSQSTYKQPTFYDFHGEFYMKGAIRTYLYHTNSWYELSKQSYYLLLFFQ
                     KLWDEKQEVEASWQNGRIQIRLVVEEEGWQVHYRFPDNQAREVERLLASGMLVAIRSS
                     RNFIKIRTSRFIAPYIALIKQRQLYRKKPSKAMLQWEKYQSQLIEIQRTYRLVEESAP
                     QTFFGHWLNKVQLYLCSAIASYWEEVIKGVEWQEAREIKDQRKIEKPHNPIEDKWKEN
                     IARRHKRKVDQLIGHDTLEKLRQLKTELDD"
     gene            complement(2404..2952)
                     /locus_tag="VCA20_001784"
     CDS             complement(2404..2952)
                     /locus_tag="VCA20_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HEP1835557.1"
                     /translation="MKNNSIKTVVATGIGAALFVVIGLLISIPTFVPNTYIQLQYAVQ
                     SLLSIVFGPIVGFFVGLIGHAFIDALRGGSPWWSWVLASAVFGLVLGFARNRLRIQEG
                     IFDGKDIFTFNIFQAVANLIAWGVIAPVLDILIYSEAANKVFAQGLVAGIANSITVAI
                     AGTLLLAVYAKSQTKTGSLSKD"
     gene            complement(2973..3821)
                     /locus_tag="VCA20_001785"
     CDS             complement(2973..3821)
                     /locus_tag="VCA20_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HEP1835558.1"
                     /translation="MSNNLLVLQSDFGLVDGAVSAMIGVALQESRDLVVHHLTHDITP
                     YNIFEGSYRLFQTVEYWPEGTTFVSVVDPGVGSKRKSVVALTEQNHYIVTPDNGTLSF
                     IKKYVGIKAVREISEVANRRANTEHSYTFHGRDVYAYTGAKLASGHISFEEVGPELSV
                     DKIVEIPTVPTEVGSDYVKGAIDILDVRFGSLWTSITREEFYTLQPQFEDRFEVTIYN
                     NDMLVYQNQVTYGKSFADVRIGQPLIYINSLYRVGVAINQGSFAKAYNVGVGQNWHIE
                     IKRISN"
     gene            complement(4316..5032)
                     /locus_tag="VCA20_001786"
     CDS             complement(4316..5032)
                     /locus_tag="VCA20_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEP1835559.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKKGDPDPETN
                     SALKFVIDRAKQAQVPKHIIDKAIDKAKGNTDETFVEGRYEGFGPNGSMIIVDTLTSN
                     VNRTAANVRSAFGKNGGNMGASGSVSFMFDKKGVVVFAGDDADAIFELLLEADVEVDD
                     VEAEDGTITVYTAPTDLHKAIVALKESGIQEFNVTDLEMIPQSEVSLEGDDLATFEKL
                     YDALEDDEDVQKIYTNVDGF"
     gene            5193..5504
                     /locus_tag="VCA20_001787"
     CDS             5193..5504
                     /locus_tag="VCA20_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000827864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEP1835560.1"
                     /translation="MKRNKHLPLTETTYYILLALLEPAHGYHIMQKVEDMSDGDVKIA
                     AGTMYGAIENLLKLKWICSVPSSDKRRKVYQVTATGQEIIKLETQRIQKLHHIAKELG
                     F"
     gene            5515..6141
                     /locus_tag="VCA20_001788"
     CDS             5515..6141
                     /locus_tag="VCA20_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HEP1835561.1"
                     /translation="MIRHKLFTSLQEEEHWINSIQSEGYQLIKVTPWTAAYHFEKCSR
                     PPHPVRLDFHEHIAKGEYSNYLSLFEDCGWQPIQGSRRCGIHYFQQTIEASSTEIFSD
                     TESKKAFYSRYQNYAYSYFGLFLTLFFIHYQVGLQNGYTLWNPKSWYLTPGLWERTGT
                     SFWFGFLFETPFALFRSGIIPLFFLAWSIYFLHIAEKGKKEAKKLEKY"
     gene            complement(6213..8381)
                     /locus_tag="VCA20_001789"
     CDS             complement(6213..8381)
                     /locus_tag="VCA20_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEP1835562.1"
                     /translation="MKKFLSFEFWQKFGKCLMVVIAVMPAAGLMVSIGNSIPLISPES
                     ELLIRIGNIIAQIGWGIIGNLHLLFALAIGGSWAKEKAGGAFSAGLAFILINLITGHF
                     FGVTTDMLADAAATVTTVFGTEIPVSGYFVNILGQPALNMGVFVGIIAGFVGATTYNK
                     YYNYRKLPDVLTFFNGKRFVPFVVIYRSVLVALGLAIFWPVVQTGINSFGKWIASSQD
                     TAPILAPFVYGTLERLLLPFGLHHMLTIPMNYTSLGGTYDILTGAQAGTQVFGQDPLW
                     LAWITDLINLKDAGDMAQYNDLLANITPARFKVGQMIGSSGILMGLTYAMYRNVDEDK
                     KHKYKGMFISSALAVFLTGVTEPIEFMFMFAAMPLYVVYAFVQGAAFAMADIVNLRMH
                     SFGNIEFLTRTPMAIKAGIGMDVINFIWVTALFAVGMYFIANFMIKKFNLATAGRNGN
                     YDTETTDVVSNSNVDTADANSQVVQIINLLGGRDNIADVDACMTRLRVSVKDVAQVGD
                     ENAWKQAGAMGLIIKDSGVQAVYGPKADVLKSDIQDLLESGVAIPRTEIVATEEVAPE
                     TQFKGVTETVYSVAEGQAIAITEVKDPVFSQKMMGNGYAVEPSSGNVYAPVSGIVTSV
                     FPTKHAVGILSDKGVEVLVHVGLDTVALSGAPFSAKVTDGQRVEAGDLLLVADLDAIR
                     SAGRETTIVVAFTNTAEIKSVSLGNLGQVSKDSQVATVEL"
     gene            complement(8415..9206)
                     /locus_tag="VCA20_001790"
     CDS             complement(8415..9206)
                     /locus_tag="VCA20_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEP1835563.1"
                     /translation="MKLLTINVHAWLEERQDEKLDVLAKTIAEKAYDVIALQEVNQLI
                     TADLVTKDLKADNYGLILLEKLRTLGQFDYSYYWSNSHIGYDKYEEGIAFLTKLPVYE
                     VDPFYCSQNKSIDSILSRKIMGLTVFYQDQLIDLYSCHINLPGSEEEDQLDNIRTIVE
                     RTGNRRFKILMGDFNTDAFSDCEAYEAITELGLYDSYNLAMEKDQGITVEKAIDGWAG
                     HSQEKRLDYIFLNQKKEVLSSLVIFNGENRPIISDHFGVEVEINV"
BASE COUNT         2787 a         2095 c         1676 g         2777 t
ORIGIN      
        1 agaattctga ttgcacaaaa aaattgcact gatttttcaa tgcaattttg gtattactaa
       61 gcattcgctg gagccaaagt ttccccaatc acttccaagc gttctgccca ttcttgacga
      121 gtcaagccgt tttcagaaat gtaacggttg cgctcgtgag cacgacattc agctgaacag
      181 ccacgaacat acttgtgctc attctcttct gatgtcaaaa tacgacggtt acagaatgga
      241 ttgccacagt tgacatagcg ctcacatggt gtgccatcga accagtcctt accaacgaca
      301 actgggttaa catggttaac atcgaccgcg atacgctcat caaagacata catcttccca
      361 tcccacaatt caccacgaac ttctgggtct ttaccatagg ttgcgatacc accgtggagc
      421 tgaccgacat ccttgtagcc ttcacgaacc atccagcctg agaatttctc acagcgaaca
      481 ccaccagtac agtaaacaac tacgcgcttg tccatgaatt tctctttgtt atcacggacc
      541 cattgtggca actcacggaa gttgcggatg tctggacgga tcgcatcacg gaagtgacca
      601 aggtcatact cgtagtcatt acgtgtatcc aaaacaactg tatcttcgtc caagagagct
      661 tctttgaact cttttggtga caagtaagca cctgttgtta caagtggatc aatatcgttg
      721 tcaaagtcat tgtcttccaa accaaggtga acaatttctt ttttataacg aacaaacatt
      781 ttcttgaaag cttgctcatt ttcttcatca atcttaaacc acaaatcgct gaacaatggg
      841 tttgcatgaa cgtagtccat gtacttttga gttgtttcat agtcaccaga aacagtaccg
      901 ttgattccct catcagcaat caaaatacgt ccttttaatc cgatagactt acagaaagct
      961 agatgctcag cagcataggc ttcagcattt tcaattggga catacttgta atagagtaga
     1021 acgcgaatat cttttgacat agtgtctcct tttcaatctt tgcaatagat gatatagtgg
     1081 cttcaattag gaaaaggacg gctttaagtt atagtgttaa taacccgctc ctaaaataac
     1141 tttagagttc tcaaattgac aaacttagat atgtcccgtt tctaattcac ttgactataa
     1201 aactctgtca tttctaataa atgatacctt acgattatat cgtttagcac ctcaaaagac
     1261 aaaaatctga actgaaaatt tagaacctgt attcgcaggt tcagtgctta tgtgtaaaaa
     1321 atacggtaac atggatgaca tgctaccgca tttattgaag gttcgctata acttcttagt
     1381 cgtctaattc tgttttcagt tgtcggagtt tttccaaggt atcatgtcca atcagttggt
     1441 ccacttttcg cttatggcgt ctagcaatat tttccttcca tttatcttca ataggattat
     1501 gtggcttctc gattttcctt tgatctttga tttctcttgc ctcctgccac tccactcctt
     1561 taataacttc ttcccaatag ctggcaatgg cagagcagag atagagttgg accttattca
     1621 accagtgtcc aaaaaaagtt tgcggagcac tttcctccac tagccgatag gtcctttgga
     1681 tttcaataag ttgactttgg tatttttccc actggagcat agctttactc ggcttttttc
     1741 gataaagttg acgctgtttg attagagcta tatacggtgc tataaaacga ctcgtccgta
     1801 tcttgataaa atttctggag gagcgaatgg ccaccagcat gcctgaggcc aataagcgtt
     1861 caacctccct tgcctgatta tctggaaaac ggtaatggac ttgccacccc tcttcttcaa
     1921 ccactaagcg aatctgtatc ctgccatttt gccaactcgc ctccacctct tgcttttcat
     1981 cccataactt ttgaaaaaag agaagaaggt agtagctttg cttactaagt tcataccaac
     2041 tgttcgtgtg gtaaaggtag gtccgaatcg ctcctttcat ataaaattcg ccatggaaat
     2101 cataaaaagt tggctgttta tatgtagatt gtgatgggtg gttactaact cggaaaacct
     2161 tataataata attattccaa agaatgcagc cataaattga tttcgtcgtt ttcgattgat
     2221 aaagacttaa aacttcctcg tcaggattga gtatagaatg aataatcggt cccaactttt
     2281 cttctgtcca gaccatactt ctccccttga acacatgttt ttgtttgttt tatattatta
     2341 tataatatat gtattttttg gtattaaaaa aaccctagaa ttttttctag ggaaaataaa
     2401 aacttaatct ttagacaaac tacctgtctt cgtttgagat tttgcataaa cagccaaaag
     2461 gagtgtccct gcaatagcaa ctgtaatact gtttgcaata cctgctacaa gtccctgtgc
     2521 aaagacctta ttcgccgcct cactgtagat aagaatatct aaaactgggg caatgactcc
     2581 ccaagcaatg aggttagcta ctgcttgaaa aatattgaaa gtaaaaatat cttttccgtc
     2641 aaaaattcct tcttgaatac gcagacgatt tctagcaaaa cctaacacca agccgaaaac
     2701 tgctgaggct agaacccatg accaccaagg cgacccacct cgcagtgcgt ctataaaggc
     2761 atgaccaata agccctacaa agaaaccaac aattggtcca aaaacaattg acaaaagcga
     2821 ctgaactgca tactgcaatt gaatgtaggt atttggtaca aaggttggaa tactaatcag
     2881 caaaccaata accacaaaga gggctgcgcc gataccagta gcaacgactg ttttgattga
     2941 attatttttc ataactatct cctaaaattt ctttaattac taatacgttt gatttcaata
     3001 tgccagtttt gacctacacc gacattataa gccttggcaa atgagccctg attaatcgca
     3061 acacctacac gatagaggga attgatataa atcaatggct gtccgatacg tacatccgca
     3121 aaggacttgc cataggttac ttggttttga tacacaagca tatcgttatt gtatatggtc
     3181 acttcaaagc gatcttcaaa ctgcggttgc aaggtgtaaa attcttccct cgtaatcgag
     3241 gtccaaagcg agccgaatcg aacatccaga atatcaattg caccttttac atagtccgaa
     3301 ccaacttccg ttgggactgt tggaatttcc acaatcttat ccacgcttaa ttctggacca
     3361 acttcttcaa agctaatgtg tccagaagcc aatttagctc ccgtgtaagc ataaacatct
     3421 cgaccatgaa aggtgtacga atgttctgta ttggcacggc gattggccac ctctgaaatt
     3481 tctcgaactg cctttatccc cacatatttt ttgatgaagg atagcgtccc attatctggc
     3541 gttacaatgt agtgattttg ttcagtcaaa gctactacac tcttacgttt tgaaccaact
     3601 cccggatcga caaccgaaac aaatgttgtc ccctccggcc agtattcgac cgtttgaaag
     3661 aggcgataag aaccctcaaa aatattgtac ggagtaatat catgagttag atggtgaaca
     3721 actaaatctc tcgattcttg gagagcaaca ccaatcatcg ctgaaactgc accatcaact
     3781 aaaccaaaat ctgattgtaa aacaagtaaa ttatttgaca tacattctcc taatcttatt
     3841 accgttctat gataacagaa aacccgtgac cattcaaata gatatttcca aagcctggct
     3901 atagtttttc cttatacctc atctatcaag tactctacta tcagccaccg aaaactccgc
     3961 aaatttttcc atcgtccctt cttcaaacgt aagctaaaat atccccactt ctctagttcc
     4021 aaccaactat cgtctttgat caattggata aaaagaaaac cagctgccgc tggttctcat
     4081 ttccaacctt ctaggacgtc ccacgtacaa tgagaatcgt ctaccgacga tttcaatctc
     4141 caacctttta ataaaaatga ctgtcccccg gacttttgag tataaagaaa atcgcctagc
     4201 gcagttccaa tttccaacct tctaggacgt cccacgtaca atgagaatcg tctaccgacg
     4261 attccaatct ccaacctttt aatataaaaa aagtgctact agcacttttt ttatattaaa
     4321 acccatctac atttgtgtag attttttgga cgtcttcgtc gtcttctagg gcatcgtaga
     4381 gtttttcaaa tgttgcgagg tcatcgcctt caagtgatac ttctgattgt gggatcattt
     4441 caaggtcagt gacgttgaac tcttggattc ctgattcttt aagtgccacg attgccttgt
     4501 gcaaatcagt tggagctgta tagacagtga ttgtgccgtc ttctgcttct acgtcatcca
     4561 cttcgacatc tgcttcaagg agcaattcga agatggcgtc tgcatcatca ccagcaaaga
     4621 caaccacacc ttttttatca aacatgaatg atacagaacc agatgcaccc atgttaccgc
     4681 cgtttttacc aaaagcagag cgcacgttag ctgccgtacg gtttacgttt gatgtcaaag
     4741 tatcaacgat aatcatagaa ccatttggtc caaagccttc gtaacgacct tccacgaagg
     4801 tttcgtctgt gtttcctttt gccttgtcaa tggccttgtc aatgatgtgt tttggaacct
     4861 gcgcttgctt agcacggtcg ataacaaatt tcagcgctga gtttgtttct ggatctgggt
     4921 cacccttttt cgctgctaca tagatttcaa caccaaattt ggcgtaaact tttgagttag
     4981 caccgtcttt tgcggttttc ttggctacaa tattggccca tttacgtccc atggggaacc
     5041 tcctacattt tataaaatcg ctttattata ccataaatgg caaggattga gaagattttt
     5101 aaaatcttct tcactataca gttgacatgt atagttttcc gatatataat atatagtaca
     5161 actgtacatt tgaactatat aggagaggcc aaatgaaaag gaacaagcat ctccccttaa
     5221 cggagacaac ctactatatt ctattagccc tcttggaacc tgcccatggc taccatatca
     5281 tgcaaaaagt agaagatatg agtgatggtg atgtgaaaat cgctgcagga accatgtatg
     5341 gagcgattga gaatcttttg aaactaaaat ggatttgttc cgttccaagt tccgataaaa
     5401 gaagaaaagt ctatcaagta acagcaacgg gacaagagat tattaaactg gaaacacagc
     5461 gtattcaaaa actacatcat attgccaaag aattaggttt ttaaggagga agccatgata
     5521 agacacaaat tattcaccag tttacaagag gaagaacatt ggattaattc gattcaatcc
     5581 gaaggttatc agcttatcaa agtaactccg tggacagcag cctatcattt tgaaaaatgt
     5641 agtcgacccc ctcatcccgt acgccttgat tttcatgaac atattgcaaa aggagaatat
     5701 tcaaattacc tctctctatt tgaagactgt ggttggcaac ctatacaagg tagtcgtaga
     5761 tgtggcatcc attattttca acaaacaatc gaagcttcaa gcacagaaat tttttctgat
     5821 accgaatcaa aaaaagcatt ctattcacgc taccaaaatt atgcctactc ctatttcgga
     5881 ttatttctga ctttattttt tatccattac caagttggac tccaaaatgg atacacactt
     5941 tggaatccta aatcctggta cctaactcca gggctatggg aacgaactgg taccagtttt
     6001 tggtttggtt ttctctttga aaccccattc gcattgttta gaagtggcat tattccctta
     6061 tttttccttg cttggtctat ttatttcctc catatcgctg aaaaaggtaa aaaagaagcg
     6121 aagaaattag aaaaatactg atggcatact atccaaaaga aaaagattga aaagagttga
     6181 ctgcaatcat cttttcaatc tttttttact ttttacaact caactgtcgc aacttgacta
     6241 tctttgctta cttgcccaag atttccaagg ctgacagatt tgatttcagc agtgtttgtg
     6301 aaggcaacaa cgatggttgt ttcacgtcct gctgagcgaa tggcatccag atctgcgaca
     6361 aggagcaaat cccctgcttc aacgcgttga ccatctgtta ccttagctga gaatggagca
     6421 ccgcttagtg caaccgtatc tagaccgacg tgcaccaaga cttctacacc cttgtcagac
     6481 aaaataccga cagcgtgctt ggttgggaag acactggtta cgatacctga aactggtgcg
     6541 taaacattgc cagagctagg ctcaactgca taaccgttgc ccatcatttt ttgtgagaag
     6601 accggatctt tcacttctgt gatggcaatg gcttgccctt cagcaacaga gtaaactgtc
     6661 tccgtcacac ctttgaactg agtctcagga gcaacttctt ctgtcgcaac aatttccgta
     6721 cgaggaatgg ccacgcctga ttctagcaag tcttgaatgt ctgatttgag aacatctgct
     6781 tttggtccat agactgcttg aacacctgag tctttgataa tcaagcccat tgcaccagcc
     6841 tgtttccaag cattttcatc tccaacttgt gccacatctt taacactaac gcgaagacga
     6901 gtcatacaag catccacatc tgcgatatta tcacgaccac caagcaagtt gatgatttga
     6961 accacttgtg aattggcatc cgcagtatct acgtttgagt ttgaaaccac atccgttgtt
     7021 tctgtatcat agttaccatt gcgtccagct gttgctaggt tgaatttctt aatcataaag
     7081 ttggcaatga agtacatacc aaccgcaaag agggctgtta cccagataaa gttaataaca
     7141 tccataccga taccagcttt gatcgccatc ggtgtacgtg taaggaattc gatattacca
     7201 aatgagtgca tacgcaagtt gacaatgtca gccatggcaa aagccgcacc ctgtacaaaa
     7261 gcataaacga catagagtgg cattgctgca aacatgaaca taaactcaat tggttctgtt
     7321 acacctgtca agaatacggc aagtgctgaa gaaataaaca tacctttgta cttatgtttt
     7381 ttatcttcat ccacattgcg gtacatagcg taagtcaatc ccatcaaaat ccctgatgaa
     7441 ccaatcattt gaccaacttt gaaacgggct ggagtaatgt ttgcaagaag atcattgtac
     7501 tgagccatat caccagcgtc tttgaggttg atcaagtctg taatccaagc caaccagagc
     7561 ggatcttgtc caaatacttg tgtacctgct tgtgcacctg tcaaaatgtc ataggtacca
     7621 ccaagtgacg tatagttcat tggaatagtc aacatgtgat gcaaaccaaa tggaagaagc
     7681 aaacgttcca aggtaccata aacaaaaggt gcaagaattg gtgctgtatc ctgtgagctt
     7741 gcaatccatt taccaaagct gttgattcct gtttgaacaa ctggccaaaa gatagctaat
     7801 cctagcgcta caagaactga gcgatagata accacaaatg gtacaaagcg tttaccgtta
     7861 aagaaggtca aaacatcagg taatttgcgg taattgtagt atttgttata ggttgttgcc
     7921 cctacaaaac cagcgatgat accaacgaag acacccatgt tcagagcagg ctgacccaag
     7981 atgttgacga agtagcctga aactggaatc tctgttccaa aaacagtggt tacggttgct
     8041 gctgcatctg caagcatgtc agtcgtcaca ccaaagaaat gacctgtaat caagttaata
     8101 aggatgaagg caagacctgc tgagaaggca ccaccagcct tttccttagc ccagctacca
     8161 ccgatagcca aggcaaagag caagtgaagg tttccgataa tcccccaacc gatttgggcg
     8221 ataatattcc cgatacgaat caagagttct gattctgggc tgattaacgg aatagagttt
     8281 ccgatagaaa ccatcagacc tgctgcaggc ataactgcaa tcacgaccat taaacactta
     8341 ccgaattttt gccaaaattc gaaactaaga aattttttca ttttttctcc tttatttttt
     8401 ggctctgtgc cattttatac attaatttca acttccacac caaaatggtc tgaaataatc
     8461 ggacgattct ctccattgaa aatcactaag ctggataaaa cttccttttt ctgatttaag
     8521 aaaatgtaat ccaatcgttt ttcctgactg tgaccagccc agccatcgat agccttttca
     8581 accgtaatcc cctggtcttt ttccatcgct aaattgtatg aatcataaag cccgagctct
     8641 gtaatcgctt cgtaggcctc gcaatctgag aaggcatcgg tattaaaatc tcccatgaga
     8701 atcttaaaac gtctattgcc agtccgttcc acaatcgtac gaatgttatc aagctggtct
     8761 tcctcttcac tccctggaag attgatgtga caagaataca ggtcgatcaa ctggtcttga
     8821 tagaagaccg tcaagcccat aatttttcgt gataggatgg aatcaataga tttattctga
     8881 ctacaataaa atggatccac ttcataaact ggtaacttag taagaaaggc aatcccttct
     8941 tcgtacttat cgtagccgat atgtgagttg ctccaataat atgaatagtc aaattgtccc
     9001 aaggttctta atttttctaa taaaatcaag ccataattat ccgccttcaa gtctttcgtt
     9061 accaaatcag cagttatgag ctgattgacc tcttgaagag cgatgacatc ataagccttt
     9121 tcagctattg ttttggctaa aacatctagt ttttcatcct gcctttcttc taaccaggca
     9181 tggacattaa ttgttagtaa tttcattgct ccccctctct ctgaaaatgc gtaaccgttt
     9241 tctttatatc catagtatag cgtaaacgtt tgcattagtc aataactttt ccgtaatatt
     9301 ttaaactttt acgtaaaagt tgtgtattct actaa
//
LOCUS       DATUCG010000098         9276 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.100, whole genome shotgun sequence.
ACCESSION   DATUCG010000098 DATUCG010000000
VERSION     DATUCG010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9276
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.100"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(<1..335)
                     /locus_tag="VCA20_001791"
     CDS             complement(<1..335)
                     /locus_tag="VCA20_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEP1835564.1"
                     /translation="MANLLIVKAHPLDAQKSYALRALEEFQTRYASLHPEDRIEIVDV
                     FEDQIPALDKPLLEAMGAAKRGEAISPEQAEQLGRYNALTQQFLAADKIVVVNPLWNL
                     NVPSQLVSW"
     gene            complement(468..875)
                     /locus_tag="VCA20_001792"
     CDS             complement(468..875)
                     /locus_tag="VCA20_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEP1835565.1"
                     /translation="MITYKQNPQLDFQAVLDLYASVGWTGYTSRPEMLEKALEHSLLV
                     LATFDGDRLVGLLRAVGDGHSIVFIQDILVLPTYQRQGIGRQLLEQAVTHFPGIYQLH
                     LLTDNTEKTRSFYEAIGFTAVDSLDCVAYTYLK"
     gene            complement(987..1589)
                     /locus_tag="VCA20_001793"
     CDS             complement(987..1589)
                     /locus_tag="VCA20_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HEP1835566.1"
                     /translation="MKKIDIKTLFATSGIFLLIALLTIYYYGALPDTMVTHFGVNGEP
                     NGSMAKYMVTILTPLSFFCVHLFICVLYDVKGIGKTPVIRVVKWFFPLLALIIQVSLL
                     WYNLGGELDYRRLVIGLLAVYYMVIGNYLPKEELDSKTNEIERKGRKQSGYLLMIGGL
                     LQLVSLLGSPTLSILVMILVGISVVSLSIYYAYLHFKTAS"
     gene            complement(1576..1854)
                     /locus_tag="VCA20_001794"
     CDS             complement(1576..1854)
                     /locus_tag="VCA20_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEP1835567.1"
                     /translation="MGLSETLKAISAPIRRQILDGLKSGPKSAGEIVEQFQLTGATVS
                     HHLSTLKKAGLILEEKQKNFIYYRLNYTVFEEVLVWIASFGGQQDEEN"
     gene            complement(1998..3578)
                     /locus_tag="VCA20_001795"
     CDS             complement(1998..3578)
                     /locus_tag="VCA20_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEP1835568.1"
                     /translation="MKFTDLQLSEDILLAVEKAGFETPSPIQEQTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLNKIDTKNQAIQALIIAPTRELAVQSQEELFKFGREKGVKVRS
                     VYGGSSIEKQIKALRSGAHIVVGTPGRLLDLIKRKALKLDGVETLILDEADEMLNMGF
                     LDDIEAIIERVPESRQTLLFSATMPEPIKRIGVKFMKEPEHVKIAAKELTNVNVDQYY
                     LRVKEHEKFDTMTRLMDVDQPELSIVFGRTKRRVDELTRGLKLRGFRAEGIHGDLDQN
                     KRLRVIRDFKNDQIDVLVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     QSGQSITFVSPNEMGYLAIIEDLTKKRMKGLKPATAEEAFEAKKKVALKKIEREMADE
                     TIRSNFDKFKKDAIQLAAEFTPEELALYVLTLTVQDPESLPEVEIAREKPLPFKFAPG
                     QAKGKGGRGGRDRDRGGRRDGDRNRDRGGRRGDRNDRNRRDDKFKRDNRRQDNKKPHQ
                     RTSSEKKTGFVIRNKGER"
     gene            complement(4000..4593)
                     /locus_tag="VCA20_001796"
     CDS             complement(4000..4593)
                     /locus_tag="VCA20_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEP1835569.1"
                     /translation="MTQIKWRDCLLICFGAILYALVLNMLIIPHEFGEGGITGVTLLL
                     FYTTGIETSLSSFVLNGILIVLAYRFLSKLTVFYTFLAVATMSLTMHYTTFLQIKWLP
                     LIPSALLAGLVTGLSMALVIYGNGSTAGSDIIALLCNKYFKWKISTVILIFDVFVVTP
                     LAFKIGLVKTFWTLVMVVIISKSLDWFLQALKKNNHK"
     gene            complement(4671..5054)
                     /locus_tag="VCA20_001797"
     CDS             complement(4671..5054)
                     /locus_tag="VCA20_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEP1835570.1"
                     /translation="MGLFSGLLGNASQMDNDKIEQELEHVLLDNEQVEMAFSLIRDLI
                     VFTEYRLILVDKQGMTGKKVSYKSIPYRSISRFTVETSGHFDLDAELKIWISSAVEPA
                     EILQFKSDKSVIAIQKALASAVLGK"
     gene            complement(5142..7718)
                     /locus_tag="VCA20_001798"
     CDS             complement(5142..7718)
                     /locus_tag="VCA20_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEP1835571.1"
                     /translation="MKKKTTLFALTGIILSCHLFLAGCQKTENTAINHQGVQSSNISK
                     VDENTIVVSEIKSNGYMRLYGEESYFFEGPIPYAAHFLKDTLPDSDYKLNKEDIQYQL
                     EKGYIIKVNEQYFYYPKDKDANVISISEAKKLTKNQKKPTSQAKKGVAGVDYPTDDGF
                     LFENEDQILSKTDEGLVLEHNGHSHFIFYKDLKESKWSYLVPKEHIENSQSSSHSNAK
                     QAKLSQTFDDGYVFDPKDIVAEDANGYTVRHGDHYHYIWKSSLQYGQGNTGETIGKKG
                     QITVLDNHSLHTPTTRNNSVPLKATTEKGHTSTQSNSKKAFPGIDYPTSDGFLFDGSG
                     VQGQTALGLLIGHGTHTHLLPYSHLIGSPWESYIPTQYLETARAEYHSSTSTQVDVEI
                     PLSSQEGGKPNSSVEEEREAKKSYLAENLHVSKEKIKVIETDAGPAFVYPHGDHSHTI
                     LIEKVEVGKPIEDPHGDPHAHDKIGMATLKQLGFDDEIIEDILHATADTPFPSNETDS
                     EKMREWLKTVKYLNIGQRKDPLKRAGLDLMPNVEVLGIGFTPIDDVKPILQFKKLKQL
                     WMTSTGVKDYQFLKEIPTLEGIDLSQNGVSDLGFLEEFPNLKVVSAAGNDIEDITILA
                     KLKALESLNLDHNKVTDLSPLADLSQLTAVSLDNNRITDLSALQNKKKLTRLYISQNP
                     QLDISTLKTENLEELTANESNVKDLQFVKNNPNLTSLTLKNNKITELQGIEENEKLVN
                     LDVEGNQIKTLEIEGKQESVVRLNVADNQLKNLEGVNDYKALEDLNASKNDIETLAIT
                     EPNKTLKTIDVSENHIPKEELNLNDQKIPSAIAEHFPAVEGGSIENNQPKEVDKEAKE
                     SE"
     gene            8106..8858
                     /locus_tag="VCA20_001799"
     CDS             8106..8858
                     /locus_tag="VCA20_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835572.1"
                     /translation="MENTATQAFEELVAEQKKKRNRILRIIITLFTAIIVFFACLALF
                     KYNSGNISGTWRNSQAEQDIVETSIKELQNQLPGINKTWIDATYTMTVVDKKAVFTAT
                     TSFDIPKIAKLTLDQAYKVMLEEAQKEASSLGISVEEYLTTALGADYEKQLKSFLPTQ
                     AEAEKEFHKYQDEYAKELGATYNQETGTISATFFEGDVDQLFHTVTITKINDAAIKQL
                     TTYDISVKDIISYSRKGNTTIINGNEEFTLEK"
     gene            complement(8969..>9276)
                     /locus_tag="VCA20_001800"
     CDS             complement(8969..>9276)
                     /locus_tag="VCA20_001800"
                     /inference="COORDINATES: protein motif:HMM:NF012235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEP1835573.1"
                     /translation="LKCLEQVGLLDWFERQEAGFDTLLSSSKQLSGGEERRFDLARAL
                     YRDAEVLLFDEPTTGLDSENEALIAETLSNIKEKLLIVVTHSQSESFLSVFDEKVEL"
BASE COUNT         2794 a         1972 c         1599 g         2911 t
ORIGIN      
        1 atccatgaca ctaattgact tggcacattg aggttccaaa ggggattgac cacgacaatc
       61 ttatctgctg caagaaattg ttgggtcaag gcattgtagc ggcccaactg ctctgcttgt
      121 tcagggctga tagcttcgcc tcttttagct gctcccatag cctctaacaa gggcttatcc
      181 aaagctggta tttggtcctc aaagacatct actatttcaa tccgatcttc tgggtgcaaa
      241 ctggcgtacc gtgtttgaaa ctcttccaaa gcccgcaagg cgtaggactt ttgtgcgtca
      301 agtgggtggg cttttacgat taatagattt gccattggtt ctctcctttg ttatccgtta
      361 ttactaatta ttgataaata tattctactc tttttgattt gaaattcaaa tcatttgctc
      421 gaagcaaaag gctggtctcc cagcctctgc ttttttagat tgttttatta ttttaaatag
      481 gtgtaagcga cacagtccag gctatcaacg gctgtaaagc caattgcctc atagaaagac
      541 cgtgttttct cagtattgtc cgtcaagaga tgaagctgat aaatacccgg aaagtgggta
      601 acggcctgct ccaaaagctg acgcccaatt ccttgtcgct gataggttgg taggaccaag
      661 atgtcctgaa taaaaacgat tgaatgcccg tcgccaactg ctcgcaagag acccactaga
      721 cggtcaccgt caaaggtagc aagaacgagc agactatgtt ccaaggcctt ctctagcatt
      781 tctggacggc tagtatatcc tgtccaacct acagaagcat agaggtcaag gacagcttga
      841 aaatcgagtt gaggattttg tttgtaagta atcatgtctt tctcctttgt attgtttttt
      901 agaatacaaa ggctttgtcc attgttaaaa cgcccatggt ctttcctcct gttttgctac
      961 atttagtata acaaaaactg ctccaattat gaagcagttt taaaatgcag ataagcataa
     1021 tagatactaa gacttacaac agaaatacca accaatatca ttacaaggat agatagggtt
     1081 ggtgaaccca acaaacttac taattgcaac aacccaccaa tcatcaagag atagcctgac
     1141 tgctttcttc cctttcgttc tatttcattg gtcttgctat ccaattcctc cttgggtaaa
     1201 taatttccta tcaccatata atagacagcc agcagtccaa tgacaagtct ccggtaatcc
     1261 aattctcctc ctaaattata ccagagtaaa gaaacttgaa tgatgagagc cagtagcgga
     1321 aaaaaccatt tgaccactcg aatgactggt gttttcccta tccctttcac atcataaagt
     1381 acacaaatga ataggtgaac acaaaagaat gatagcggtg tcaaaattgt caccatatac
     1441 ttagccatag agccatttgg ctcaccattt acgccaaaat gcgttaccat ggtatctggc
     1501 aatgctccat agtaataaat cgtcaataaa gcaatcagta ggaatatacc agaagttgca
     1561 aataaggttt ttatgtcaat tttcttcatc ttgttgccct ccaaaacttg caatccaaac
     1621 caagacttct tcaaagaccg tatagtttag acgataataa ataaaatttt tctgcttttc
     1681 ctccaaaatt aatccagctt ttttcaaagt ggataaatga tgagaaactg ttgctcctgt
     1741 taattgaaat tgttctacaa tttccccagc agacttcggc cccgacttaa gaccatctag
     1801 aatttgccta cgaataggag cagaaattgc cttcaaggtt tcagacaatc ccataataag
     1861 cctccttgtc catctattta gaaatatttc taaatagaat tatactctgt ttttattgtt
     1921 tgtcaagaac tatttcgatt attttctaaa tagatttcaa aaaaacagac cactctttat
     1981 ggtctgtcaa atccggctta tctctcccct ttattgcgga taacaaagcc tgttttcttt
     2041 tcacttgatg tgcgttggtg tggttttttg ttgtcctgac ggcggttgtc gcgtttaaac
     2101 ttgtcatcgc gacggttgcg gtcattacga tcaccacggc gtcctccgcg atctcggttg
     2161 cggtctccat cacgacgacc accgcgatca cggtcgcgac caccacgtcc acccttgccc
     2221 ttggcttgac caggtgcaaa cttgaatggc agtggttttt ctcgcgcaat ttctacctct
     2281 ggcagacttt ctggatcctg aacagtcaag gtcaatacat aaagcgctaa ctcttcaggg
     2341 gtaaattcag ccgctaactg aatagcatct ttcttgaact tatcaaaatt gctacggatg
     2401 gtttcatccg ccatttcacg ttcaatcttc ttcaaagcaa cttttttctt ggcttcaaag
     2461 gcctcttcag ctgtcgctgg cttgagcccc ttcatacgct tcttggtcaa atcttcgatg
     2521 atggccaagt aacccatctc atttggagat acaaaggtaa tagactgacc agactggcca
     2581 gcacgacctg tacgaccgat acggtgaaca tagctttctg ggtcttgtgg aatatcgtag
     2641 ttatagacgt gggttacacc tgagatatcc aaaccacggg ccgcaacgtc cgtcgcaaca
     2701 agaacatcaa tctggtcatt tttaaaatca cggatgacac ggaggcgctt gttctggtcc
     2761 aaatctccgt ggataccctc tgcacggaag ccacgcagtt tcaaaccacg agtcaattca
     2821 tcaacacggc gcttagtacg accgaagacg atagacaatt ctggctggtc aacgtccata
     2881 aggcgtgtca tggtgtcgaa tttttcatgt tctttgacac gaagataata ttggtcaaca
     2941 ttgacattag tcaattcctt tgctgcaatc ttaacgtgct caggttcttt catgaacttg
     3001 acaccaatac gcttgattgg ctctggcatg gttgcagaaa acagaagggt ttgacggctc
     3061 tctggcacac gttcgatgat cgcttcaata tcatccaaga agcccatgtt gagcatttca
     3121 tctgcctcgt caagaatgag agtttcaaca ccgtcaagtt tgagggcctt gcgtttgatc
     3181 aagtccaaga ggcgacctgg tgtaccaaca acgatgtgag cacctgaacg aagggccttg
     3241 atttgctttt caatgcttga gccaccgtaa accgagcgaa cttttacacc cttttcacga
     3301 ccaaatttga aaagctcttc ctgactctgc accgccaact cacgcgttgg agcaataatc
     3361 aaggcttgaa ttgcttgatt ttttgtatca atcttgttga gggttggcaa accaaaggct
     3421 gcggttttcc ctgtccccgt ctgcgcttga ccaatcacat cttttccttc cagagcaagc
     3481 ggaatggtct gctcctggat tggtgatggc gtttcaaaac cagccttctc aacggcaagc
     3541 aaaatatctt ctgataattg taaatctgta aatttcaatg tattctcttt tctaaaaggc
     3601 agtgcgaagc taccttatct aacttttttg taacaagata tcagggtcga accactgata
     3661 ttggatttgc aacttaccaa gtataacaca atctaacaat aatagttaat atttaccttt
     3721 aaaaagtcaa taatacgttt tcatgaggag tccatcattt tatagacatt tgaattagct
     3781 ttgatacatc gatactttcg gtttgcccta ctctagtacc agctccttgt ctgaaagtta
     3841 tactaaaatc agacattgtt gatttgattt gatgaacttc agttctatct gcatctgcgt
     3901 cgcctagtct gtttttgatt ttcattgagt attaattcat tcgactataa gaaaagagaa
     3961 acctagttat acatgcacaa ctaggtttct cttgtatttt tatttatgat tattcttttt
     4021 caatgcttgc aagaaccagt ccaaactttt actgataatg acaaccatta ccaatgtcca
     4081 aaatgttttt acaagcccaa ttttaaaagc caaaggagtt accacaaata catcaaaaat
     4141 gagaataacg gtggaaatct tccatttgaa atatttatta cacaacaatg caataatatc
     4201 acttcctgcc gtggacccgt ttccataaat gactagagcc attgataaac ctgtcaccaa
     4261 tcccgccaag agagcagagg gaatcagggg caaccatttt atttgcagaa aagtcgtata
     4321 atgcatggtt aaggacatag tagcaaccgc taagaaagta tagaaaaccg ttaacttact
     4381 taaaaagcga taggctaata caatcaaaat cccattcaaa acaaaactgg acagacttgt
     4441 ttcaatccct gtcgtataaa acaaaagtag ggtaacacca gtaatcccac cttcgccaaa
     4501 ttcatgtgga atgatgagca tattcaaaac aagggcatat aagattgcac caaaacatat
     4561 caataagcag tctctccatt ttatctgcgt catattctct cctcacaaac agtccacctt
     4621 agtatatcaa aaaaagcaga aagcggaaag ccactttctg tacttttttc ctattttcct
     4681 aagacagctg aagccagagc tttctgaata gcaatgacac tcttatcgct cttaaactga
     4741 agaatttcag caggttcaac agccgaggaa atccatattt tcagctcggc atctaagtca
     4801 aaatgaccag aggtttctac cgtaaagcgt gaaatcgagc ggtaaggaat cgatttataa
     4861 gatacctttt tcccagtcat tccttgctta tcgaccaaaa tcaatcgata ttctgtaaaa
     4921 acaatcaaat cacgaatcag actaaatgcc atctcaacct gttcattatc cagcagaaca
     4981 tgctccaatt cctgttcaat cttatcatta tccatttgcg aggcattacc tagtaaacct
     5041 gaaaatagtc ccataatatg actcctttgg tatttttcta tagtataaca caaaaagcta
     5101 ctagttgact atcttaggta gctctttacc aaacacgaga attattcgct ttcctttgct
     5161 tccttatcaa cttctttagg ttgattgttc tcaatactgc caccttcaac tgctggaaaa
     5221 tgttctgcaa tcgcagatgg gatcttctga tcatttagat ttaattcttc cttcggaatg
     5281 tgattttcac tcacatcaat tgttttcaaa gtcttatttg gctctgtgat ggcaagtgtt
     5341 tcaatatcat tttttgaagc attcaggtct tctaaggctt tgtaatcatt gactccttct
     5401 aaattcttca attgattatc tgcaacattt aatcgaacaa ccgattcttg tttaccctct
     5461 atttccaaag tcttaatttg attcccctct acatccagat ttactaattt ttcattttct
     5521 tctatccctt gtaattcagt aatcttatta ttcttaagag taagactagt taaatttgga
     5581 ttgtttttta caaactgaag atcttttaca ttactttcat tagctgtcag ttcttctaaa
     5641 ttctctgttt ttagagttga tatatccaat tgaggattct gtgatatata caaacgggtt
     5701 aacttcttct tgttttgaag cgcagataaa tctgtgatac gattattatc taaactaaca
     5761 gctgtcaatt gtgacaaatc tgctaatggg gataaatccg tcactttatt atgatctaaa
     5821 tttaaagatt ccaaagcttt taatttagct agaatagtga tatcttcaat gtcatttcct
     5881 gcagcagaaa ctacttttaa gtttggaaat tcttccaaaa agcccaaatc agaaacacca
     5941 ttttgtgata agtcaattcc ctccaatgtt ggaatttctt tcaaaaactg ataatcctta
     6001 acgcctgtgc tagtcatcca taattgtttc aattttttaa attgcaagat aggttttaca
     6061 tcatcaattg gcgtaaaacc aatccctaac acttcaacat ttggcatcaa gtccagaccg
     6121 gctctcttta gcggatcttt tcgctgacca atatttagat attttaccgt ttttaaccac
     6181 tctctcatct tctcggaatc tgtttcatta gatgggaaag gagtatcggc cgtagcatgc
     6241 aaaatatctt caataatctc atcatcaaaa cctaattgtt ttaaagtagc cattccaatt
     6301 ttatcatgag catgtgggtc accgtgaggg tcttcgattg gttttccaac ttcaactttc
     6361 tcaatcaaga ttgtatgaga atggtcacca tgagggtaaa cgaaagcagg tcctgcatct
     6421 gtttctatca ccttgatttt ttcttttgaa acatgcaggt tttcggctaa gtagctcttt
     6481 ttagcttctc gttcttcttc tacacttgag ttaggtttcc caccttcttg tgatgacaac
     6541 ggaatttcta catcaacttg agtggaagtt gacgaatggt attctgctct agcagtctct
     6601 aaatactgag taggaatata agactcccac ggacttccta tcaaatgact gtacggaagt
     6661 aaatgagtat gagtaccatg gccaatgagt aaccctaacg ctgtttgacc ttggactccg
     6721 ctaccatcaa aaaggaaacc gtcgcttgtt ggataatcaa tacctgggaa agctttcttt
     6781 gaattagact gagtggaagt atgccctttt tctgtggtag ccttgagtgg gacagaatta
     6841 tttcttgtgg tcggagtatg caacgaatga ttatcaagaa cagtaatctg tccctttttt
     6901 ccaatggtct ccccagtatt tccttgtcca tattgcaaac ttgatttcca aatataatga
     6961 taatgatctc catgacgtac cgtatagcca ttcgcatctt cagccactat atccttcgga
     7021 tcaaatacat aaccatcatc aaaagtttga gatagcttcg cttgctttgc gttggaatgc
     7081 gagctggatt ggctattctc aatgtgctct ttgggtacca gataggacca tttgctttct
     7141 tttaaatcct tatagaaaat aaaatgtgaa tgaccattat gttctaagac cagtccctca
     7201 tctgtttttg aaagaatttg atcttcattt tcaaaaagaa atccatcatc cgtaggataa
     7261 tcaacaccag ctaccccttt tttagcttga gaagtaggtt tcttttgatt tttggtgagt
     7321 ttcttagctt cagaaataga aattacatta gcatccttat cctttggata gtagaagtac
     7381 tgctcattta cttttataat ataacctttc tctagctgat actgaatatc ttctttattc
     7441 aatttataat cagaatctgg aagggtatct tttaaaaagt gtgccgcata cgggatagga
     7501 ccttcaaaaa agtatgactc ttctccatat agacgcatat agccattaga tttaatttca
     7561 gacactacaa tcgtattttc atctactttt gagatattgc tactttgaac accttgatga
     7621 tttattgcag tattctctgt tttttggcaa cccgccaaaa aaagatgaca agataggata
     7681 atgcctgtca aggcaaataa ggttgttttc tttttcattt ttgtcctcct aagcttatta
     7741 accagttaac aatttactag tatacatttt gtttttgatt tttgcaagaa aaaacaactg
     7801 acaatgattg ttcatgaata aattttccat tccatggcaa acgcatgaaa aaatttgttt
     7861 tataaaccat attttcacga ctgctgtcag tttttagtag aaaaaatgta atataatagc
     7921 cttggaaatg agggagtatc cttgttaaag tgcaggtttt acaaaaacct ttaaaaaaat
     7981 gcatgagaca aaaaatagtt acatgccttt ttcttcatac agatagcgtg tttccatccc
     8041 tacaaccatt gacaaagcaa acaaactata gtatattaaa aatagggaaa taaaaggaga
     8101 atattatgga aaacacagct actcaagctt tcgaagaact cgttgcggaa cagaaaaaga
     8161 aacgtaatcg catccttaga attattatca cactctttac cgctattatc gtattttttg
     8221 cctgtcttgc attattcaaa tataatagtg gcaacattag cggtacgtgg cgcaattccc
     8281 aagctgaaca agatattgtc gaaaccagta tcaaggaatt gcaaaatcaa cttccaggta
     8341 tcaataaaac gtggattgat gcaacttata cgatgacagt cgtagataag aaagctgtct
     8401 tcaccgctac aacaagcttt gatattccta aaatcgcaaa attaaccttg gatcaagcct
     8461 ataaggtgat gttagaggaa gcacaaaaag aagcaagttc tctgggaatt tccgtagaag
     8521 aatacttaac aaccgctctt ggagcagatt atgaaaagca attgaaatca tttctaccta
     8581 cacaagctga ggcggaaaaa gaatttcata agtaccaaga cgaatatgct aaagaactag
     8641 gtgccacata caatcaagaa actggtacta tctctgctac ctttttcgaa ggagacgtcg
     8701 atcagctctt ccataccgtg accattacaa aaattaatga cgcagcaatc aaacagctga
     8761 ctacatacga tatttccgta aaagatatta tttcctattc tcgcaaggga aataccacca
     8821 ttattaacgg aaatgaagaa tttactctag aaaaataaca aaacaaggac gagttgtaca
     8881 aagctcgtcc ttttataatt ttcatatcaa gatgtagcat atatactcat aagtttgtaa
     8941 tgcttaattg ctttaaggac tttaaacatc ataactcaac cttctcatca aaaacggata
     9001 agaatgattc actttgtgaa tgagtaacta ctatcaacaa tttctcttta atattagaaa
     9061 gagtctctgc aataagagcc tcattctcac tatctaatcc cgttgttggt tcatcaaata
     9121 gtaagacctc cgcatctcta taaagggccc gcgccaaatc aaatctccgc tcttcaccac
     9181 ccgatagttg tttagaggag gataaaagcg tatcgaatcc agcctcctgt ctctcaaacc
     9241 agtccagcaa ccctacttgt tctaaacatt tcaggg
//
LOCUS       DATUCG010000099         8808 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.101, whole genome shotgun sequence.
ACCESSION   DATUCG010000099 DATUCG010000000
VERSION     DATUCG010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8808
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.101"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(454..1335)
                     /locus_tag="VCA20_001801"
     CDS             complement(454..1335)
                     /locus_tag="VCA20_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEP1835574.1"
                     /translation="MPLVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKQAP
                     VLIQTSMGAAKYMGGYKVARNLIANLIESMNITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLAKAKEVVELAHAKGISVEAEVGTIGGEEDGIVGSGELAPIEDAV
                     AMVATGIDFLAAGIGNIHGPYPANWEGLDLDHLRKLTEAVPGFPIVLHGGSGIPDAQI
                     QEAIKLGVAKVNVNTECQIAFANATRKFAAAYEANEAEYDKKKLFDPRKFLADGVKAI
                     QASVEERIDVFGSANKA"
     gene            complement(1570..1655)
                     /locus_tag="VCA20_001802"
     tRNA            complement(1570..1655)
                     /locus_tag="VCA20_001802"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1619..1621),aa:Leu,seq:aag)
     gene            complement(1943..3547)
                     /locus_tag="VCA20_001803"
     CDS             complement(1943..3547)
                     /locus_tag="VCA20_001803"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEP1835575.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGFKVTIQKFDPYI
                     NIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITDALKDKIKRAARTTDADVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGSDNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEQPAG
                     QGIKNKLAQFCDVAPEAVIESLDVEHLYQIPLNMQAQNMDQIVCDHLKLDVPPADMTE
                     WTAMVNKVLNLQKKVKIALVGKYVELQDAYISVVEALKHSGYANDAAIELDWVNANDL
                     TAENVAERLGQAQGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGVCLGMQLTCVEFA
                     RHVLGLEGANSFELDPETKYPIIDIMRDQIGVEDLGGTLRLGLYPSKLKRGSKAAAAY
                     DNQEVVQRRHRHRYEFNNDFREQFEKAGFVFSGVSPDNRLVEIVEIPENKFFVACQYH
                     PELQSRPNRPEGLYTAFVSAAMENEK"
     gene            complement(3740..4321)
                     /locus_tag="VCA20_001804"
     CDS             complement(3740..4321)
                     /locus_tag="VCA20_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEP1835576.1"
                     /translation="MELNVFAGQEKSELSMIEVARAILEERGRDNEMYFNDLVNEIQN
                     YLEKSNSEIRATLPTFYSDLNVDGSFIPLGENKWGLRSWYAIDEIDEEVITLEEDDED
                     APKRKKKRVNAFMVGDEDAIDYGHDDPEDEDNYPGSASSEYDDENPDDEKDEVESYDS
                     EMNEIIPDDELDEEDVDLGEDDDEYSDEEVVDE"
     gene            complement(4448..7603)
                     /locus_tag="VCA20_001805"
     CDS             complement(4448..7603)
                     /locus_tag="VCA20_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEP1835577.1"
                     /translation="MKKKAVVGSVAALVLGMSLCSYQLGRFQAMEEQKNRVSYIEDSQ
                     SVQTTVAEQLAPDQVSAKENIDAEQIVVKITDQGYVTSHGDHFHYYNGKVPFDAIFSE
                     ELVMKDPNYVLQDSHIINEVQDGYVIKVDGKYYLYLKDPSKHKNVRSKEEVERQKGIS
                     SADSKNRAAGNSKDGRYRTDDGYVFNPTDVIEDTGDGFIVPHGDHFHFIPKKDLSVAE
                     LKAAQDYWNQKGSASSASGSQYGDRNNRAQQITISAGQGQDLASLLAQLDATPLSQRH
                     VEADGLVFDPRTITKKTAAGVIVPHGDHYHFIPYSQMSPLEEKISRMIGVNGAGVSSG
                     AQASHSQHTPTQPNRPVTPIGTVTTQPVSPTQPVLPTQPQQSTGKVVSYMGRQIPAYG
                     KGLDGKAYFTSDGYTFSKDSITSVDDQGLIASHGDHFHYVGFGELEDFEIKQVEEWVN
                     EKAGKQVPPKTSEQVGNDAKPTTPSQGNDSKPVKPIQEENRPAFEYKQVTAKRKLAGK
                     VVYEMEVGGKTYTYGRDELDLMKISFAELTLAEKDKQYIFDIAPLAEGDLKPAMLVGM
                     DQIPMKGANATYDTGQSFIIPHIDHIHVLPYTWLSKEQIATIRYIMQHPEIRPSAWTT
                     SGHGDGEATDLVPPILNATPKANRLGLKNWQIIHTAEEVMDARAKGKFATNDGYIFSA
                     EDVLDPASFVFSQAFSLPRATGGSLRSISKKDLSKEELEAVQTLLDKRDAEELAKNVT
                     PIEKRAGLKNWQIVHSAEELAEAKAAGKYTTKDGYIFDPADLLDPKVKIGTDNYRIPR
                     VITDGYRRINKSDLNYLSELIPAEAMVAQREKSDSSSPSTPAPTETGASAGETTMPEQ
                     PQVAKETAEEIYNRVEAKKVVPFEALTYNAGYATEVRNGTLVIPHQDHYHYVSFKWFD
                     QGSARSPEGYSLEDFLATVKYYMTNPQERPVSDDGWGVFTPNTPSESTEETETEESDE
                     EIISEETEEIDEFTEELKRRAEEFGMDFKTFEQSLVTLSDRYKVSFEAFEYDATSKVV
                     RLVDKDGVKRTISLPSLEEQV"
     gene            complement(7637..8557)
                     /locus_tag="VCA20_001806"
     CDS             complement(7637..8557)
                     /locus_tag="VCA20_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEP1835578.1"
                     /translation="MFKKIVGTCLLALFGFVLAACSAQKEATQVQPGMKIVTSFYPIY
                     SLVKEVSGNKNDVRMIGSRQGIHSYEPSAADIKAIYDADVFIYHSRILESWAGRLEPN
                     LQGSSVKVLEASTNLPLTKVPGLEDVEAGQGIDEASLYDPHTWLDPVLVGQEAVAIGE
                     LLAESDPKNADYYRQNAATLEEKAQKLADKYSPIFLKATSKTFVTQHTAFSYTAQRFG
                     LKQLGIAGVSEEEPSPRQLAEIKEFVDTYNVQTIFTEKGASDKLAKALASSTGVDLKV
                     LDPLEADPENNLTYLENLEQVLETLAQELK"
BASE COUNT         2295 a         1957 c         1634 g         2922 t
ORIGIN      
        1 aacaaatcgc tcatataaag cgagaacttg ggtaaagatt tttgaaaaaa taacttgtgt
       61 ttgattggga tttgcgagat aataatgttg gctttgcata tcattccctc atttcgtggt
      121 tgttttgagt ttatgatacg caaagtgttt ttatttagct agaattaact agcacaacgg
      181 gtatattcat aggtaaatat tttaacacta cagtttgcgc cccatccata attcttcgtt
      241 ctctaagtta ttacagtcat gaaaactttg gagcgtttta tattttaact gttctctgct
      301 cacttctatc gtagatttct tcatggcaaa gaactagatc aaacttaatc aactattata
      361 ataaaaagga ttctcgaaag aaaatccttt ttggtttaat ctaagaaact tatgctttgt
      421 ttgcttgaaa tctgagattt cttcgcagtt acattaagct ttgttcgctg aaccgaatac
      481 gtcgatacgc tcttcaacag atgcttggat agctttcacg ccgtctgcca agaatttacg
      541 tgggtcaaag agttttttct tatcgtattc tgcttcgttt gcttcataag ctgctgcaaa
      601 cttacgagtt gcgtttgcaa atgcgatttg gcactcagtg tttacgttaa ctttagcaac
      661 acccaatttg attgcttctt ggatttgtgc atctggaata cctgaaccac cgtgcaatac
      721 gattgggaaa cctggtacag cttcagtcaa tttacgcaag tggtcaaggt caagaccttc
      781 ccagtttgct gggtatggac cgtggatatt accgatacct gctgccaaga aatcgatacc
      841 agttgcaacc attgctactg cgtcttcgat tggagccaat tcaccgctac ctacgatacc
      901 atcttcttca ccaccgatag taccaacttc agcttcaact gagatacctt tagcgtgtgc
      961 caattctaca acttcttttg cttttgcaag gttttcttct actggaaggt gtgaaccgtc
     1021 aaacatgata gaagtgtaac caacttcgat acattcaagt gcatcttcgt agtgaccgtg
     1081 gtcaaggtga atagcaactg gaactgtaat gttcattgat tcaatcaagt ttgcaatcaa
     1141 gttacgagct actttgtaac cacccatgta cttagctgca cccatagaag tttggataag
     1201 aactggagcc tgttttgctt ctgctgcacg caagatagct tgagtccact caaggttgtt
     1261 tgtgttaaat ccaccaactg cataaccgtt gtcacgcgct gcttggacaa atttttctgc
     1321 tgaaactaat ggcatttcga tagcctccta atattttttg aggttgcacc tcatactctt
     1381 ctattctacc atttttattt tgaaaatgct agttgaaacc gtctagtttt gagaaaactt
     1441 tcacatatct tttcatgaaa gccataataa tgcctgtttc gtatcattct tgtaatttat
     1501 aaactagcat atatcgcgct ctgcacttat ctagacaaag caaaaagact agccttagct
     1561 agtccactat gcggagagtg ggacttgaac ccacacgacc taaagcggtc acaggatcct
     1621 tagtcctgcg cgtctgccaa ttccgccatc cccgcttaac acaagtacta gtatatcatt
     1681 aggaaccagc cttgtcaaca ctttttttga attttatttt ttattttcac cttttttgcc
     1741 aaaacgtatc gatttagctg attcttttgc aatattctac tatatataat gcttaaaacc
     1801 atctagacaa tgatgccata tgggggtggt atttcgtatt ttcttctata aatactgaca
     1861 aaatactgac atcttttcgt ttctttatat aaaatataga agaagattga atagtaccaa
     1921 tcttcttcta taacggttat acttattttt cattctccat agctgctgaa acaaaggctg
     1981 tatacaaacc ttcagggcga ttaggacgag attgtagttc tggatgatat tggcaggcaa
     2041 cgaagaattt attttctgga atttcaacaa tttctaccaa acggttgtct ggtgacacgc
     2101 ctgagaaaac aaaacctgct ttttcaaatt gttcacggaa gtcattgtta aactcataac
     2161 gatgacgatg gcgacgttgt acaacttctt gattatcata agcagctgca gccttggagc
     2221 cacgtttgag cttgcttgga taaagaccta aacggagagt tcctcctaaa tcttccactc
     2281 caatttgatc acgcatgatg tcaataattg gatatttagt ttctgggtct aattcaaagc
     2341 tgttagcacc ttctaaaccg agaacgtgac gagcaaattc tacacaggtc agctgcattc
     2401 ccaagcagac ccctagcatt ggcacgtcat tctcacgagc ataacgaatg gcttgtattt
     2461 tcccttctgt accacgttgt ccaaagccac caggaacgat aattccttgt gcttgtccta
     2521 gacgttcagc tacgttttct gctgtcaaat catttgcatt gacccagtcc aattcgatag
     2581 cagcatcatt tgcatatcct gagtgtttta gagcttcaac tacagaaata taggcatctt
     2641 gtaattctac atacttacca actaaagcga tttttacttt tttctggaga ttaagaacct
     2701 tgtttaccat cgctgtccac tctgtcatat ctgctggagg tacgtcgagc ttcaagtgat
     2761 cgcaaacaat ttgatccatg ttctgcgctt gcatatttaa tgggatttgg taaagatgtt
     2821 cgacatccaa ggattcaata actgcttctg gagcaacatc acagaattgt gccagtttat
     2881 ttttaattcc ttgtccagca ggctgctctg tccgaattac caacatgttg ggctgaatac
     2941 ctagaccacg caactctttt acagaatgtt gtgttggctt agttttcatt tcgcctgctg
     3001 ctttgagata aggcaagagt gttgtatgga tatacataac attatctgaa ccgacatctg
     3061 ccttcatctg acgaagcgct tcaaggaatg gcaagctttc aatatcacct actgtaccac
     3121 ccacttccgt gatgatgaca tctgcatctg tagtacgagc tgcacgcttg attttgtctt
     3181 tcaaagcatc tgtaatgtgt ggaataactt gaactgttgc ccctaagtat tcccctttac
     3241 gctctttacg gaggacttcg ctataaattt tacctgttgt aacatttgaa tatttgttca
     3301 ggttaatgtc gatgaaacgc tcataatgcc caaggtccaa gtcggtttct gcaccgtcat
     3361 ccgtcacaaa aacttcaccg tgttgatacg gactcattgt tcccgggtca atgttgatgt
     3421 atggatcaaa tttttggatt gttaccttga aacctcgatt cttcaataaa cgacctaggc
     3481 tggctgctac aatccctttg ccgatagagg aaacaacgcc acctgttaca aaaatatact
     3541 ttgtcatgca aactcctttt ctacgattca ggctaatagg aagcaatttg tggggatact
     3601 tcctgttaac ttgaagaaaa accaaaaata gctccctatc gctagggagc tccgacctca
     3661 aaagaggtgc ccgataatat cttatcgtaa ttcagtagtc ttgtcaaatt atttttaaga
     3721 atctatgggg atataattct tattcgtcga cgacctcttc atctgaatat tcgtcgtcat
     3781 cttcacctaa atcaacatcc tcttcatcca attcgtcatc tgggataatt tcgttcattt
     3841 ctgaatcata ggactcaact tcatcttttt cgtcatctgg attttcatca tcgtattctg
     3901 atgatgcact acccggatag ttgtcctcat cttctggatc gtcatgtccg tagtcaattg
     3961 catcctcatc acctaccatg aaggcattga cacgtttctt cttgcgtttt ggtgcatctt
     4021 catcatcttc ttcaagagtg atcacttctt cgtcgatctc atcgattgcg taccaagaac
     4081 gtaagcccca tttgttttcg ccaagtggaa tgaagcttcc atccacgttc aaatcagaat
     4141 agaaggttgg gagggttgcg cgaatctcgc tgtttgattt ttcaaggtag ttttgaattt
     4201 cattgaccaa gtcgttgaaa tacatttcgt tgtcacgtcc acgttcttcc aaaatagcac
     4261 gggctacttc aatcatggaa agttcgcttt tttcttgtcc tgcaaatacg ttaagttcca
     4321 aggctgttct ccttttttgt ctttcctttc tattttacgc taaaaaaggc aaatagtcaa
     4381 agataatctt tatcgttttt ctgtaaaaaa atacctgcag agctcagcgc tacaggtatt
     4441 ttgtttacta tacttgttct tctaaacttg gaagggagat ggtccgtttg accccatcct
     4501 tatctacaag tcgaacaact ttgcttgttg catcatattc gaaagcttca aatgagactt
     4561 tgtaacggtc tgagagcgtg accagacttt gttcaaaggt tttgaagtcc atgccgaact
     4621 cttctgctct tcgctttaat tcttctgtaa actcgtcaat ttcctcggtt tcttcagaaa
     4681 taatttcttc atcactttct tccgtctctg tttcttctgt tgattcagat ggtgtatttg
     4741 gtgtaaatac tccccagcca tcgtctgaaa cagggcgttc ttgtggatta gtcatgtagt
     4801 acttaaccgt tgctaggaaa tcttctaaac tatacccttc aggacttctt gcactacctt
     4861 ggtcaaacca tttaaaggat acatagtgat agtgatcttg atgcggaatc actagagtcc
     4921 cgttgcgtac ttcggttgca tagccagcat tgtaggtcag tgcttcgaaa ggaacgactt
     4981 tcttcgcttc cactcggtta taaatttcct ctgctgtttc tttggcaact tgtggctgtt
     5041 ctggcattgt tgtctcgcct gcacttgctc ctgtttcagt tggtgcaggt gttgacggtg
     5101 aactagaatc agatttctca cgttgagcca ccatagcctc tgctgggatc agctcgctta
     5161 agtaattcaa atcactcttg ttaatccgac ggtaaccatc tgtaatcaca cgtgggatgc
     5221 gatagttatc tgtacctatt ttcactttgg gatctaatag gtctgctggg tcaaaaatat
     5281 agccatcttt tgttgtatat tttcctgctg ccttagcttc tgccaactct tctgctgagt
     5341 gaacaatttg ccaatttttc aaacctgcac gtttctcaat cggtgtgaca tttttcgcca
     5401 attcttcagc gtctcgttta tcaagaagag tttgtacagc ttccaattct tctttagata
     5461 aatctttctt ggaaattgaa cgtagagaac ctcctgtcgc tcttggtaga ctgaacgctt
     5521 gactaaagac aaaacttgct ggatctagta cgtcttccgc cgagaagata tagccatcat
     5581 ttgtcgcaaa tttccctttt gcacgtgcat ccatgacttc ttctgcagtg tgaatgattt
     5641 gccaattttt caagcctaag cggttcgctt taggtgttgc attcagaatt ggtggaacaa
     5701 gatccgttgc ttctccgtca ccatgtccac ttgtagtcca agcagatgga cgaatttcag
     5761 gatgttgcat gatatagcgg atcgttgcaa tttgttcctt actcaaccat gtgtaaggta
     5821 ggacgtggat gtgatcaatg tgtggaataa taaaggattg tccagtatca taggtcgcat
     5881 tcgctccctt catcggaatt tgatccatgc cgaccaacat ggctggctta agatctcctt
     5941 ctgcaagcgg tgcaatatca aagatatact gcttatcttt ttctgccaag gtcaactcag
     6001 caaaagagat tttcatcaaa tctagttcgt cacgaccata cgtataagtt tttccaccta
     6061 cttccatttc ataaacaact tttccagcca atttacgttt agccgttact tgtttgtatt
     6121 caaatgcagg gcggttttct tcttgaattg gttttacagg ttttgaatca ttcccttgac
     6181 ttggtgttgt gggtttagca tcgtttccta cttgttcaga tgttttaggt ggcacttgtt
     6241 tgcccgcctt ctcattaacc cattcctcta cttgcttgat ttcaaaatct tcgagttcgc
     6301 caaaaccaac ataatggaaa tgatctccat gactagctat caacccttga tcatcaacag
     6361 aggtaataga gtctttgcta aaggtgtatc catcacttgt aaaatatgcc ttaccatcta
     6421 acccttttcc gtaagcagga atttgacgtc ccatatagga aaccactttc cctgttgact
     6481 gctgcggttg tgttggtaat actggttgtg taggcgatac cggttgcgtt gtaaccgtgc
     6541 cgattggtgt tactggacga tttggttgtg ttggtgtatg ttgggagtga cttgcttgtg
     6601 caccagaaga aacaccagct ccatttacac caatcattcg agaaatcttc tcttctagag
     6661 gagacatctg gctgtatgga ataaagtggt aatggtcgcc gtgtggaacg atgacacctg
     6721 ctgcggtttt cttcgtaatc gttcttgggt caaataccaa tccatctgct tctacatggc
     6781 gttgagatag gggtgttgca tccaattgag ccaataaact agccaaatcc tgaccttgcc
     6841 ccgcactaat tgttatctgt tgagctctat tgtttctatc gccatattga ctaccactag
     6901 ccgagctcgc acttcctttt tgattccagt aatcttgagc agccttcaat tcggcaactg
     6961 ataaatcttt tttaggaata aagtggaaat ggtcgccgtg cggaacgata aaaccatctc
     7021 ccgtatcttc aataacatct gtcggattaa agacatagcc atcgtctgtt ctgtagcggc
     7081 catctttact atttcctgct gcccgattct tactgtcagc agaagagatt cctttttgcc
     7141 gctccacttc ttctttgcta cgaacatttt tatgtttgct agggtctttt agatagagat
     7201 agtatttccc atcaactttt atgacgtagc catcctggac ttcattgatg atgtgactgt
     7261 cttgtaagac atagtttggg tccttcatga ccaactcttc actaaaaatc gcatcaaagg
     7321 ggactttacc attatagtag tggaaatggt caccatgtga tgtcacatag ccttgatcag
     7381 taattttaac gactatctgc tcagcgtcaa tattttcctt ggcagaaact tgatcaggtg
     7441 ctaattgttc agcaacggta gtttgtacac tttgactatc ttcaatatag gacacacgat
     7501 ttttttgctc ttccatcgct tggaaacgtc caagctgata gctacagaga ctcatcccta
     7561 aaacaagggc ggctacggag ccaacaactg cttttttctt cattttttct cctttcggtc
     7621 gatagaattc ggccttttat tttaactctt gagctaatgt ttctaagact tgctctagat
     7681 tttctagata agttaaatta ttttctggat ctgcttcaag aggatccaac acttttaaat
     7741 ctacacctgt cgaactcgcc aatgccttgg caagcttatc cgaggcacct ttttctgtaa
     7801 aaattgtttg cacattgtag gtgtcaacaa attctttaat ttccgccaac tgtctaggac
     7861 ttggttcttc ctctgaaact cctgcgatcc ccaactgctt caacccgaag cgttgcgcag
     7921 tgtaagagaa ggctgtgtgt tgagtgacaa atgtttttga tgtggctttt aaaaagattg
     7981 ggctatactt gtctgccaac ttttgcgcct tttcctctaa agttgcggca ttttgacgat
     8041 aataatccgc attcttagga tcactttctg ctaaaagttc accaattgca acagcttcct
     8101 gaccaaccaa aaccggatcc agccatgtgt gggggtcata taaactagct tcatcaatcc
     8161 cttgaccagc ttccacgtct tctaaccctg gaacctttgt cagcggtaaa tttgtcgaag
     8221 cctccaaaac cttgacagat gaaccttgca aattaggttc caagcgtcct gcccaggatt
     8281 ccaaaattcg cgaatgatag ataaaaacat ctgcatcgta tatggccttt atgtccgcag
     8341 ccgatggttc ataagagtgt atgccctgtc ttgagccaat cattcgaaca tcatttttat
     8401 tccctgacac ttccttaacc agtgaataaa tcgggtaaaa tgaggtgact attttcattc
     8461 ctggctgcac ttgagtcgct tccttctgag ccgagcaagc tgctaaaaca aaaccgaata
     8521 aggccaaaag acaggtccct actattttct taaacataat tacctccttc tttaaccagt
     8581 taattatata acaagaacat taactggtca agcatttttt tgagaaagtc atagctatcc
     8641 gcgaaaacgt gatttgtatc cctagccata aacttctcct ccagcgacat ctaaaaactt
     8701 tcaccgaact tcattcaagt taatactata acaacttggc taatatcctt aacaacttac
     8761 ttttatcttg ttctaaaact gccaattcat acaacttttt cttttgaa
//
LOCUS       DATUCG010000100         8742 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.102, whole genome shotgun sequence.
ACCESSION   DATUCG010000100 DATUCG010000000
VERSION     DATUCG010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8742
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.102"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(<1..159)
                     /locus_tag="VCA20_001807"
     CDS             complement(<1..159)
                     /locus_tag="VCA20_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEP1835579.1"
                     /translation="MKIDDLRKSSNIEDRRGQSSSNSSFSSGSSGGNVLLGLLFSRLG
                     WKGKLLVLV"
     gene            383..1726
                     /locus_tag="VCA20_001808"
     CDS             383..1726
                     /locus_tag="VCA20_001808"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEP1835580.1"
                     /translation="MVEYLSVSHLTKYLKLKFDRDPYLEKVYLTGQVSNFRKRPSHQY
                     FSLKDDKAVIQATMWAGVYKNLGFELEEGMKINVIGRIQLYEPNGSYSIVIEKAEPDG
                     IGALAIKFEQLKQALTQEGLFKEEFKQALPRFTKKIGVITSPSGAVIQDIITTVSRRF
                     PGVEIVLYPTKVQGDGAAQEVVTNIQRANERDDLDVLIVGRGGGSIEDLWAFNEEIVV
                     RAIFESRIPIISSVGHETDTTLADFVADRRAATPTAAAELATPVTKADLLGYLNQQEN
                     RAYQAMTNRLKFLRGQLEKISQSVMFRQPERLYDGYLQKLDRLTNQLQTRMKELYSQQ
                     KQASLLLQQRLQGVHPGRKVESFQERIIQQERLLKSNMANIYDNKMAKADKLIEALTM
                     LDTSRIVARGYAMIRQDGRVIDSVENVQMGENLTIQMKDGQLDVEVKHVQTDENI"
     gene            1701..1916
                     /locus_tag="VCA20_001809"
     CDS             1701..1916
                     /locus_tag="VCA20_001809"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002963102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEP1835581.1"
                     /translation="MSKQTKTFEENLAELEGIVTKLERGDVALEEALAEFQKGMVLSK
                     DLQKTLAEAEKTLVKVMQADGSEAEMD"
     gene            1916..2785
                     /locus_tag="VCA20_001810"
     CDS             1916..2785
                     /locus_tag="VCA20_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEP1835582.1"
                     /translation="MVASKLQRLEEAMADFYQPQVAERLNQSVLYSLQAGGKRIRPLL
                     LLTILESFGMELKQAHYQVAACVELIHTGSLIHDDLPAMDNDDYRRGRLTNHKVYDEA
                     TAILAGDSLFLDPFGFLAQVDLPAETILALVRELSQASGTFGMVAGQVLDMAGEGRKL
                     SLEQLQAIHANKTGKLLTFPFVAAGLIAGQSDVVISHLRQAGQLIGLAFQVRDDILDV
                     TATFEEIGKTPQKDVLAEKSTYPALMGLEASKVFLEDSLNQAINELDQVAKQVPFSAE
                     KIKEIIESLRING"
     gene            2778..3599
                     /locus_tag="VCA20_001811"
     CDS             2778..3599
                     /locus_tag="VCA20_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEP1835583.1"
                     /translation="MAKERVDVLAFKQGLFETREQAKRGVMAGLVISVINGERYDKPG
                     EKIDEGIELKLKGEKLKYVSRGGLKLEKALQVFQLSVEGQTTIDIGASTGGFTDVMLQ
                     AGAKQVYAVDVGTNQLAWKLRQDERVISMEQYNFRYAELGDFELGQPSFASIDVSFIS
                     LSLILPALHRILAEGGQVVALVKPQFEAGREQIGKNGIVKDKSVHLKVLEDVTKMAVE
                     TGFSVKALSFSPVQGGHGNIEFLAHLEKSTQPNQIDKEVITTTVYQAHEEFKKDE"
     gene            3592..4017
                     /locus_tag="VCA20_001812"
     CDS             3592..4017
                     /locus_tag="VCA20_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEP1835584.1"
                     /translation="MNKRERLEVIKDLVVRFPIDTQEEIVERLEAMGVNATQATVSRD
                     IKELGIIKVPSADKGYIYGLPKAGLVKVKSQNVFEVSVMDKMINLRLVPGSSAVVKRQ
                     ILEQFQEHVFSVIADDDSILLIVTNEGIISQIEQTVRGW"
     gene            4020..5681
                     /gene="recN"
                     /locus_tag="VCA20_001813"
     CDS             4020..5681
                     /gene="recN"
                     /locus_tag="VCA20_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEP1835585.1"
                     /translation="MLLEVSIKNFAIIEQVSLNFENGMTILSGETGAGKSIIIDAMNL
                     MLGARATTDVIRHGAAKAEIEGLFSFENSRALEQILLEQGIEVADELIIRREILQNGR
                     SVSRVNGQMVNLSVLKQIGQYLVDIHCQHDQEELMKSQHHIRLLDSFGEDEFWSLKDR
                     YQTTFDAYRSLRKRVLEKQKNEQEHKARIEMLEYQIAEIEAADLKFGEDIQLNQERDK
                     LLNHKQIADTLTNAYALLDNEDFSSLNNLRSAMSDLQSLEEFDPDYKQLSSSLTEAYY
                     VVEDVTKRLSDVVDNLDFDGNRLLQLESRLDLLNTITKKYGGTVDDVLDYFSKISEEY
                     NLLTGNDLSGDDLEVQLKNLEKELVERAGQLSQSRHELAVVLEDIIRQELQDLYMEKA
                     RFQVRFTKGKFNREGNETVEFYISTNPGEDFKPLVKVASGGELSRLMLAIKSAFARKE
                     GKTSIVFDEVDTGVSGRVAQAIAQKIYKIGQYGQVLAISHLPQVIAIADYQFFIEKIS
                     DEHSTVSRVRLLTKEERIEEIAKMLAGDKITDAARNQAKELVEKG"
     gene            5775..6608
                     /locus_tag="VCA20_001814"
     CDS             5775..6608
                     /locus_tag="VCA20_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEP1835586.1"
                     /translation="MSKIKIVTDSSTTIEPSLVEELNITVVPLSVMVDGVVYSDNDLK
                     EGEFLELMRGSKNLPKTSQPPVGLFAETFADLAKDGSQIIAILLSHALSGTVEAARQG
                     ATLSGADVTILDSSFTDQAEKFQVVEAARMAQAGASKEEILAAIETVKEKTELYIGVS
                     TLENLVKGGRIGRVQGMLSSLLNIRVIMEMKDHQLTPIVKGRGNKTFKKWLEEFTATL
                     ADKKVAEIGISYSGTADFANEMKSQLQEYVSKEISVLETGSIIQTHTGEDAWAILIRY
                     E"
     gene            6601..7434
                     /locus_tag="VCA20_001815"
     CDS             6601..7434
                     /locus_tag="VCA20_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEP1835587.1"
                     /translation="MNNRKLIQGFVFFFLAILGSIFLFHQLIPVASSRISRQELTVSE
                     KISFRYVALGDSLTEGVGDTTGQGGFVPLLAQSLTNDYGYEVDYKNFGVSGNTSNQIL
                     KRMKEDGELISYLKTADLLTLTVGGNDLRKAIIKNIANLKVSTFDKPAKDYGKRLDTI
                     IKIARKNNPNLPIYVVGIYNPLYLNFPELTEMQTIIDNWNKMTEQITEKYQHVYFVPI
                     NDLLYKGLEGEAGISQNGSQVTNNLLYEEDSFHPNNTGYEIMKKAISERMNETTESWE
                     P"
     gene            7406..8002
                     /locus_tag="VCA20_001816"
     CDS             7406..8002
                     /locus_tag="VCA20_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HEP1835588.1"
                     /translation="MRPRKAGNLNIWKWLFLAQLALLIGCGIVLYTRIQTDREDLTKL
                     VQTSTEDTKVGTFSTNREQLNQTLTNYLKEYQTEEFSYQLFVTSQQVVFEGSYQIFGV
                     TIPLYIYFQPSKMEDGSILLRITEISVGSLSLPKAEVLAYLQKNYKLPAFVKIDSEQA
                     QVQVQLTELKNKFGLYGKANTIDLYNDQFIVDIYRKRQ"
     gene            8092..8367
                     /locus_tag="VCA20_001817"
     CDS             8092..8367
                     /locus_tag="VCA20_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEP1835589.1"
                     /translation="MANKQDLIAKVAEATSLTKKDSAAAVDAVFGAVADYLAAGEKVQ
                     LIGFGNFEVRERSARTGRNPQTGAEIKIAASKVPAFKAGKALKDAVK"
BASE COUNT         2760 a         1609 c         2002 g         2371 t
ORIGIN      
        1 gacaagaaca agtagttttc ctttccaacc aagtcgtgaa aagagtagtc ccaatagaac
       61 atttccgcca gatgaaccag acgagaaaga tgagttagag ctggattgtc ccctgcggtc
      121 ctcaatgtta gaactttttc gtaaatcatc aattttcata taaatttcct tagaacctgc
      181 ataatgattc ttagaaccca tattcacaag taaagtgttt ttacgcattg gactgtgaat
      241 ctaggttctt atattctcac aaactattat atagcaaatt gttgaaaaaa cacaggaaag
      301 ctatcggaaa taataggcct agttctgtat aaaagtgcaa aatttggtat aatagtgggg
      361 atacaatata gaaggagtgg acatggttga atatttatcg gttagtcatc taactaagta
      421 tttaaaatta aagtttgacc gcgatccgta tttggagaag gtgtatttga caggtcaagt
      481 gtccaacttc cgtaaacgcc ctagccatca atatttttcg ctcaaggatg acaaggcagt
      541 catccaagcg accatgtggg caggcgtcta taagaatctg ggctttgagc ttgaagaggg
      601 aatgaaaatc aatgtcattg ggcgcattca gctctatgaa ccaaatggtt cttattccat
      661 tgtcattgaa aaagctgagc cagatggtat tggagccttg gcaatcaagt ttgaacagct
      721 caaacaagct ctgacacagg aaggtttatt taaagaagaa ttcaagcaag ccttgccacg
      781 gtttaccaag aaaatcggtg tcattactag tccaagtgga gctgttattc aggatattat
      841 tacaaccgtt agccgacgtt ttccaggcgt agagattgtt ctctatccga ccaaggtgca
      901 aggagatggt gctgctcagg aggttgttac taatattcaa agagccaatg agcgtgatga
      961 tttggatgtc ctcattgttg gtcgtggtgg tgggtctatc gaagacctgt gggctttcaa
     1021 tgaagaaata gtggttcggg ctatttttga atcccgtatt ccaatcattt ccagtgtggg
     1081 tcacgaaacg gatacaactc tggctgattt tgtggcagac cgcagggcag ccactccgac
     1141 agctgccgca gaattagcga ctcctgtgac caaggcagac ttactgggtt atttaaatca
     1201 gcaggaaaat cgagcctatc aggccatgac caatcgtttg aaattcctaa gaggtcagct
     1261 tgaaaaaatt agccagtcag tcatgttccg ccagcctgaa cgcttgtatg atggttattt
     1321 gcaaaaatta gaccgcttga ccaatcaatt gcagacacgg atgaaagaac tctacagcca
     1381 gcagaaacag gctagtctct tactacagca acgattacaa ggtgtgcatc caggtcgtaa
     1441 agtagagagt ttccaagaga gaattatcca gcaggaacga ctcttgaaga gcaatatggc
     1501 taatatctat gacaataaga tggccaaggc ggacaagctg attgaggcct tgactatgct
     1561 ggataccagt cgcatcgtag cccgtggcta cgccatgatt cgtcaagatg gtcgggtgat
     1621 cgatagtgta gaaaatgtac aaatgggtga aaacctaacc atccagatga aggatggtca
     1681 acttgatgtg gaggtaaaac atgtccaaac agacgaaaac atttgaagaa aacttagctg
     1741 aattagaagg aatcgtaacc aaattggagc gaggcgatgt ggctttggaa gaagcacttg
     1801 ccgagtttca aaaaggaatg gtcctatcga aagacctaca aaagacacta gcagaagctg
     1861 aaaagacctt ggtcaaggtc atgcaggcgg atggcagtga agcggagatg gattaatggt
     1921 tgccagtaaa ttgcagcgac ttgaggaagc catggcagac ttttaccaac cccaggtagc
     1981 tgaacgcctc aaccaatcgg ttctttattc gctacaggct ggtggtaaac gaatccgccc
     2041 cttgctttta ttgaccatct tagagtcctt tgggatggaa ttaaaacaag ctcattacca
     2101 agtggcagca tgtgtcgagt tgatccatac gggaagtttg attcacgatg acctgcctgc
     2161 tatggataat gatgattacc gtcgtggtcg cttgaccaac cacaaggttt acgatgaagc
     2221 aacagctatt ttagcagggg acagtctctt tctagatcca tttggattcc tagcccaagt
     2281 tgatttacca gctgagacca tccttgctct cgtgagagag ctttcccagg catctgggac
     2341 ctttggaatg gttgctggtc aagtattgga tatggcaggc gagggacgaa aattatctct
     2401 cgaacaatta caagccatcc atgccaataa aactgggaaa ttattgacct ttccctttgt
     2461 cgcagctggt ttgattgcag gacaatcaga tgtggtaatc agccatttgc gacaagcggg
     2521 tcaattaatt ggtctagcct ttcaagttcg ggatgatatt ttggatgtga cagccacttt
     2581 tgaggaaatc ggaaaaactc cccaaaaaga tgtattggct gagaagtcta cctaccctgc
     2641 cctaatggga ttggaagcct caaaagtctt tttagaagat agcttgaatc aagcaatcaa
     2701 cgagctagat caagtggcta aacaagtccc attttcagca gaaaaaataa aagaaatcat
     2761 agaaagttta cgaatcaatg gctaaggaaa gagtagatgt actagctttt aaacaggggc
     2821 ttttcgagac gcgggagcag gccaaacgcg gtgttatggc tggcttagtt atatccgtca
     2881 ttaacgggga acgctacgac aaaccaggtg agaagattga cgagggaatt gaactcaagc
     2941 tcaaggggga aaaactcaag tatgtcagcc gtggtggtct caaattggag aaagccttgc
     3001 aggttttcca attatcagtt gagggtcaaa caacgattga tattggggct tcgacaggcg
     3061 gatttacgga tgttatgttg caggctggtg ccaagcaggt ctatgctgtg gatgtaggga
     3121 ccaatcaact ggcttggaaa ttacgtcagg atgagcgtgt aatcagcatg gaacagtaca
     3181 atttccgcta tgctgagttg ggagatttcg aacttggtca accaagtttt gcgtccattg
     3241 acgtcagctt tatctcattg agtctgattt tgccagcact ccatcgtatt ttagcggagg
     3301 gcggtcaggt tgttgccctt gtcaaacccc aattcgaggc tggacgtgaa cagattggga
     3361 aaaacggcat tgtcaaggat aaatccgttc acttgaaagt cttggaagat gtgacaaaaa
     3421 tggccgtgga aacaggcttc tcagtcaaag cccttagctt ttctcctgtt caaggcggtc
     3481 atggcaatat cgaattttta gcgcacttag aaaaatcaac acaaccaaac cagattgata
     3541 aagaggtcat aacaactaca gtctatcaag cacatgagga atttaaaaag gatgaataaa
     3601 cgagaacgat tagaagtcat taaagatttg gtagtccgat ttcctattga tacacaggaa
     3661 gaaattgttg aacgtctaga agcaatgggt gtcaatgcga cacaagcaac ggtttcacgt
     3721 gatatcaagg aacttggcat tattaaggtt ccatcagcgg ataagggata tatttatggt
     3781 ttaccaaagg caggtcttgt taaggtaaaa tcgcagaatg tttttgaagt ctcagtgatg
     3841 gataaaatga ttaatctccg tctggtacct ggaagctctg ctgtggtcaa acgccagatt
     3901 ttggaacaat ttcaagaaca tgttttttca gtgattgctg atgatgacag tattctgctc
     3961 attgttacaa acgaaggtat tatttcgcaa atcgaacaga cggttagagg gtggtagcca
     4021 tgttactaga agtgtccatt aaaaacttcg ccattattga gcaggtatca ctgaattttg
     4081 aaaacgggat gaccatccta tctggtgaaa caggtgcagg taaatccatt atcattgatg
     4141 ccatgaacct gatgctaggg gcgcgtgcga cgacagatgt tatcagacat ggggccgcca
     4201 aggctgagat tgaagggctc ttttcctttg aaaatagcag agctctcgag cagattttgc
     4261 ttgaacaggg gattgaagta gctgacgagc tcattatccg ccgtgaaatc ctacaaaatg
     4321 gccgttcggt cagccgtgtc aatggacaaa tggtcaattt atctgtcttg aaacagattg
     4381 ggcaatattt agtagatatt cattgtcaac atgaccagga agaattgatg aaatcccagc
     4441 accatatccg tcttttggac agtttcggag aagatgaatt ttggagtctt aaagaccgtt
     4501 atcagacaac ctttgatgcc tatcgtagtc ttcgcaaacg agttcttgaa aagcaaaaaa
     4561 atgagcaaga acacaaggcg cggattgaga tgttggaata ccaaattgct gaaatcgaag
     4621 cagcggattt gaagtttggt gaagatattc aactcaatca ggaacgcgat aaactgctca
     4681 accacaaaca aattgcagat acactgacca atgcctatgc actgttagac aatgaagatt
     4741 tttcaagctt gaacaaccta cgctcagcta tgagtgactt gcaaagtctg gaagaatttg
     4801 atccagacta caaacagctc tcttctagcc tgacagaagc ttattatgtc gttgaagatg
     4861 tgaccaagcg tctcagcgat gtggtggaca atctagactt tgacggcaat cgtctcttgc
     4921 aattggaaag tcgtctggat ttgctcaata ccattacgaa gaaatatggc ggaactgtcg
     4981 atgatgtttt ggactatttt agtaaaatca gtgaagaata caatctattg acaggcaatg
     5041 atttatctgg tgatgatttg gaagttcagc ttaagaactt agagaaagaa ttggttgaac
     5101 gagcaggtca gctcagccaa tcacgccatg aattggctgt tgttttagaa gatattattc
     5161 gccaagaatt gcaagacttg tatatggaga aagctcgttt ccaagttcga tttactaaag
     5221 gaaaatttaa tcgagaagga aatgaaacag tagaatttta catctccacc aatcctggcg
     5281 aagacttcaa gcctctggtt aaggtggctt ctggaggaga attgtctcgc ttgatgttgg
     5341 ccattaagtc agcctttgcc cgtaaggaag gcaagacttc catcgtcttt gatgaggtgg
     5401 atacaggggt gtctggacgt gtggcccagg caattgccca gaaaatttat aaaatcggac
     5461 aatatggtca agtattggcg atttctcatc taccacaagt cattgctatt gcagattatc
     5521 agttctttat tgagaaaata tccgacgaac attcgaccgt ttctcgtgtc cgtctcttga
     5581 ccaaagaaga gcgtattgag gaaattgcca agatgttagc cggtgacaaa atcacagatg
     5641 cagcccgtaa tcaagcaaaa gaattagtag aaaaaggtta ggcgactagc ctttttgctt
     5701 atgtccaaag tttatttttt agttaaaatt tgttataata gatggcaaaa ttgaagagaa
     5761 ttgtaggatg aaatatgtca aagattaaga ttgttacgga ttcaagtacg actatcgaac
     5821 ccagtttggt cgaagaattg aatataacag ttgttccatt atctgtaatg gttgacggag
     5881 tcgtatactc tgacaacgat ttaaaagaag gagaattctt agagctcatg cgtgggtcga
     5941 aaaacttgcc aaaaacaagc cagcccccag taggactctt tgcggaaaca tttgctgact
     6001 tggctaaaga tggcagtcag attattgcca ttctcttgtc acatgccttg tcagggacag
     6061 tagaagctgc tagacaagga gccacccttt ctggtgctga tgtgacaatc ctcgattcaa
     6121 gctttacaga tcaggctgaa aaattccaag ttgtagaagc agcacgcatg gcacaagctg
     6181 gtgccagcaa agaagaaatc cttgcagcta ttgaaacagt caaagaaaaa acggaattat
     6241 acatcggtgt atcaacccta gaaaatctag tcaagggtgg tcgtattgga cgtgttcaag
     6301 ggatgctgag tagcctactc aatatccgtg tcatcatgga aatgaaagac catcagctga
     6361 ctccgattgt caaaggacga ggtaataaga cctttaaaaa atggctggaa gagttcacgg
     6421 caactctagc agataaaaaa gtagccgaga ttggtatttc ctattcagga acggctgatt
     6481 ttgcaaatga aatgaagagt caattacaag agtacgtctc aaaagaaatt tctgttttag
     6541 agacaggttc catcattcaa actcatactg gtgaggatgc ctgggctatt ttgattcgtt
     6601 atgaataaca ggaagttgat tcaaggcttt gttttctttt ttctggcaat tctagggtcg
     6661 atttttctat ttcatcaact cattcctgtt gcctcatctc gcatctccag acaagagctt
     6721 accgtatcag aaaaaatcag ttttcgatat gtggcacttg gtgattcttt gacagaaggt
     6781 gttggagata caactggaca ggggggcttt gttcccttgt tggctcaatc tctaactaac
     6841 gattatggtt atgaagtaga ttataaaaat ttcggagttt ctgggaatac cagtaatcag
     6901 attcttaagc gaatgaagga agatggagag ctgataagct acttaaaaac agcagatctg
     6961 ttaactctga cagttggtgg caatgatttg cgcaaggcta ttattaaaaa cattgccaat
     7021 ctcaaagttt caacttttga taagcccgca aaagattacg gaaaacgatt ggatacgatt
     7081 attaagatag cgcgtaagaa taatccaaat cttcccatct atgttgtcgg gatttacaat
     7141 ccactctacc tcaattttcc tgaattgaca gaaatgcaga caattattga taattggaat
     7201 aagatgacag aacagataac tgaaaaatat caacatgtct actttgttcc gattaatgat
     7261 ttgctctata aaggattgga aggcgaggct ggtattagtc aaaatggtag ccaagttacc
     7321 aataatttac tgtatgagga agacagtttc catcccaata atactggata tgagattatg
     7381 aaaaaagcga tatcggagag aatgaatgag accacggaaa gctgggaacc ttaatatttg
     7441 gaaatggcta tttttagccc agttggcttt attgattggt tgcggaatag tcctctatac
     7501 acggattcaa acagatagag aggatttaac aaagcttgtt cagactagta cagaagatac
     7561 caaggttggg accttctcca ctaacaggga acagctcaac caaaccctca ctaactatct
     7621 aaaagagtat cagacggaag agttttccta tcaattattc gtaacgagtc agcaagttgt
     7681 ttttgaaggc agctatcaga tttttggtgt gactatccca ctctatattt attttcaacc
     7741 tagcaaaatg gaagatggca gtattttgtt acggataaca gaaatatcag ttggaagttt
     7801 atccttgccg aaagcagagg tattagctta tttacaaaaa aattataaac tacctgcctt
     7861 tgtaaaaata gactcagaac aagcgcaagt acaggttcaa ctgacagaac tgaagaacaa
     7921 atttggtctg tatggaaagg cgaatacgat tgatttgtac aacgaccaat tcattgttga
     7981 tatctacaga aaaagacagt aaaatagcat taaaaacttg accaaacgcc taaaattcgg
     8041 tactatatag aagtgagtaa taactcagaa aattattgga ggacttagca aatggctaat
     8101 aaacaagatt tgattgcaaa agttgcagaa gcgacttcat tgactaaaaa agactcagca
     8161 gcagctgttg atgcagtatt cggagcagta gcagactacc ttgcagctgg tgaaaaagta
     8221 caattgatcg gttttggtaa ctttgaagtt cgtgagcgtt cagcacgtac aggtcgcaac
     8281 cctcaaacag gtgcagaaat caaaatcgca gcatcaaaag ttccagcatt caaagctggt
     8341 aaagctctta aagacgctgt aaaataataa aaaggtccag tagacctttt tattatttgc
     8401 ttgaaaactg gggatagact gttttatccc tcgcctaaaa acaaaagagc gaggctaagc
     8461 ataaaaaagc ctaccaaggg tttaaaacaa aaataatttg aggacagtag ccaactatca
     8521 gttattgtcc tcttttcttg tgctaagcag cgataaaagt gcacacaaaa ggaacacctc
     8581 atctagcctt tttaaaaata aggtgttaga atagtatggc gtaaccaact ttattgattt
     8641 tgagttaaag tgtaatcgta aagtttgtta tgcgttatga ggtaatacat tgtcctgatg
     8701 aggcgatgta tagaggcaat cgtgtgtggc ttcgtagaag tc
//
LOCUS       DATUCG010000101         8713 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.103, whole genome shotgun sequence.
ACCESSION   DATUCG010000101 DATUCG010000000
VERSION     DATUCG010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8713
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.103"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(90..1256)
                     /locus_tag="VCA20_001818"
     CDS             complement(90..1256)
                     /locus_tag="VCA20_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEP1835590.1"
                     /translation="MKKQSPFIIAGIVMLGIVMRAPFTALPAILTDVAAGLGVEVSSL
                     GILTSIPLIMFALCSSLAPRLAAKFGMEKLMALVLLVMVLGSGMRVLNLPALYIGTML
                     VGATIAFINVLLPSLVAANFPKKIGLYTTIYITLMGVAATVASMIAVPIVSSSSWEFF
                     ILLITGLVFMAFLIWLPNVKNNHRFASENQGNQKSSIWKNKAAIAFLIFGGLQSVLYY
                     TEITWLPTISQSVGFSKAEAGLMAGFFNMTAIPMSMIIPAVLSRQTKEMRRNIMLAIS
                     SVTLLGLVMMALIPTNLILWSALHIILSFSNAALFPYMMLSFTLKTSNSQATAQLSGM
                     VQTGGYLIAAFGPGLLGYSYPIFGNWMPLILALAIVTLAMMWTIVLIEREDIIL"
     gene            1459..2607
                     /locus_tag="VCA20_001819"
     CDS             1459..2607
                     /locus_tag="VCA20_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HEP1835591.1"
                     /translation="MTTSRFQLVGSLLRPANLGEFKRQIEAREDIKYPFYDDFDGYKE
                     TEASLIQKIVAEQKENGLDIVTDGEFGRSMWHLDFLWGFDGIERYIAEHGYPFKDHDG
                     QIFETRKDIGLRITAPLSAKNHHFIDIFKQVKELAGETVTKQTIFGPAHAFNELTIFN
                     GQVGPNQVYKTRDELKAGLIAVYKEFLDQYKEAGGQIVQFDDCLWELFDPANPVPFLP
                     QDDSEALAALADEFVAINNAVIDYGHEIGLTVWTHNCRGNYESRSAAGGTYEAIAEKF
                     LRDQKYDRFFLEWDDERAGDLKALESLRDKNVEVVLGLLSSKTSDWDDEERVYKLLEE
                     ASKIIPKERLYLSHQCGFASCDSGNELAIPQQWAKIKQGQEIAEKFWS"
     gene            complement(2765..3628)
                     /gene="rsmI"
                     /locus_tag="VCA20_001820"
     CDS             complement(2765..3628)
                     /gene="rsmI"
                     /locus_tag="VCA20_001820"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEP1835592.1"
                     /translation="MKVQKSFKGQTSFGTLYLVPTPIGNLQDMTFRAVQILKEVDIIA
                     AEDTRNTGLLLKHFEVETKQISFHEHNAHEKIPVLIDWLKSGQSIAQVSDAGLPSISD
                     PGHDLVGAAIAEDIPVVALPGASAGITALIASGLAPQPHIFYGFLPRKSGQQKEIFQE
                     KKTYPETQIFYESPYRVADTLENMLTVYGDRQVTVVRELTKLYEEYQRGSISELLEYL
                     EENPLKGECLIIVEGAGEEVYPSADEVDLKAEVEREIASGIKPNQAIKEVAKRYQLKK
                     QEVYDIYHGLG"
     gene            complement(3630..3947)
                     /gene="yabA"
                     /locus_tag="VCA20_001821"
     CDS             complement(3630..3947)
                     /gene="yabA"
                     /locus_tag="VCA20_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEP1835593.1"
                     /translation="MDKKEIFDALDDFSQNLLTTLAEVDAIKKHLQGVIDENTTLRLE
                     NSKLRERLEKEDKTGHKSSNFGKENLENIYEDGFHICTFSYGQRRDNDEPCMFCVELL
                     NRD"
     gene            complement(3967..4851)
                     /locus_tag="VCA20_001822"
     CDS             complement(3967..4851)
                     /locus_tag="VCA20_001822"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEP1835594.1"
                     /translation="MKIEELRNLQPNVFQRFVTILEQRRLAHAYLFSGDFASFDMAIF
                     LSQSLFCQEKEGVLPCQKCRSCRLIEANEFSDLTVIAPQGNVIKTDTIRELVKNFSQS
                     GFESTKQVFIIRDAEKMHANAANSLLKVIEEPQSAIHIFLLTNQEEAVLPTIKSRTQI
                     IGFPKNIPAMERMLEEEGLLKTQANLLAQLVSSQEEAKKLAENKNFLDLMGQTKKFTD
                     LLLVEPTRAYLQVGILVSLAVEKAEQGRLFDLLSLILSEKMMESANVREKMDAVLKAK
                     KMWQANVSLQNSLEYITL"
     gene            complement(4848..5486)
                     /locus_tag="VCA20_001823"
     CDS             complement(4848..5486)
                     /locus_tag="VCA20_001823"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEP1835595.1"
                     /translation="MRNGFFITFEGPDGAGKTTVLQQLLPALQELGPEVVTTREPGGV
                     AIAEEIRNIILNPANTAMDDKTELLLFIAARRQHLKEKILPPLAQGKLLLIDRFIDSS
                     IAYQGFGRGLDIDDIHWLNQFATDGLKPDLTLYFDIDTEEGLARIARNADRDVDRLDM
                     EKADMHRRVRQGYLNILEQEPGRFVKIDASKPLEAVVADALAVIKKRFAERI"
     gene            5661..6527
                     /locus_tag="VCA20_001824"
     CDS             5661..6527
                     /locus_tag="VCA20_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEP1835596.1"
                     /translation="MVRLKNILLILLGAGLFAFGLNYLIMPNRLFEGGATGLTLIIYY
                     LFHIQPWIMNIVINIPLFILGWKILGKKTLYLSILGTFSVTFWLAIFEKIHFSINLQQ
                     DLILVSILGGILMGLGLGTIFRAGGTTGGSDIIARIGHKFLPYSIGEIILAVDILILT
                     LIVIVFKDLRTVLYTLMMVAIASKVIDFVTEGGYGSKGVMIVSQKSDQLAQAIDCEIE
                     RGVTFIKAQGFYSKADVNMIYSVIYKSQLQEMKELIHRIDPHAFITITDAHEVLGEGF
                     TLDRDKKPIERH"
     gene            complement(6564..7835)
                     /locus_tag="VCA20_001825"
     CDS             complement(6564..7835)
                     /locus_tag="VCA20_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HEP1835597.1"
                     /translation="MSGFNFDIDQIANNSLSTKDKTTELIQATPTGDNSKVSFLEQLT
                     LEQQTGIRAKAPQLVDNFLANQNALLDFGKEAVEEVNATVNHILSEQKKIKIPQVDEL
                     LANTNRELNGFVAKYKDISTTAELEKKPGFFQRLFKQTKNDLQEFYFDSQTIEKKMDS
                     MAASVVKQEEVLARNIVSAELLIENNTKSIENLVGVIAFIEATGQEAAERAKLAQENL
                     ATLVPTTVDYQVASEKLARVTEVANILEQQHSEYMSRLYVAWTTTPQMRNLVKVSSDM
                     KQRLGMLRRNTIPTMKLSIAQLGILQQSIKSSQTADAIVNANNAALQMLADTSKEAIP
                     MMERTAQNPTLSVASVTKLAESLVAQNNGIIAAIDEGRQKRRELEVAIVRSAETINDS
                     VKLRDQKIIAALLEQGKEAQFEAEQPLETNQ"
     gene            complement(7841..8668)
                     /locus_tag="VCA20_001826"
     CDS             complement(7841..8668)
                     /locus_tag="VCA20_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835598.1"
                     /translation="MGHKYKKYKKYGKNNSGLVALLVLLAIGFVMSFWYILLPIGLLI
                     GYFYKREAIQRFLNRDSIKRLQVLALSIRSGYEKHQELLVEKGEEKGVLQLRNRLLGQ
                     LFELDQLYQKVGKYLNGYESKEVVDTLQLKYHLKLPEAKIERNTDTQVSTGGNAVMDE
                     QGIIADLAPEILETYCNIQRDNLVILEKLEKATDKREELTAIHEANMNRFNDILQGYL
                     KIKKSPKDYFNAEERLVQAKSAMEMFDKDLDDTIKQFNEADMKDFEVSLRMMKREEE"
BASE COUNT         2524 a         1883 c         1591 g         2715 t
ORIGIN      
        1 aaatgtccag ctttttttgt ttgcaaaaaa tcccttagaa gctctgccat tttcacagaa
       61 aaatgccagc cttcttaagg aattatactt tataagataa tatcttctct ttcaatgaga
      121 acaatagtcc acatcatggc aagtgtgacg atagccagag caagaatcaa tggcatccag
      181 tttccgaaga ttggatagct gtaaccgaga aggcctggtc caaatgctgc aatgagatag
      241 ccacctgtct gcaccattcc tgataactgg gcagtagcct ggctgttgct tgttttcaaa
      301 gtaaagctca acatcatata agggaagagg gctgcatttg aaaaacttaa aataatgtga
      361 agtgctgacc aaagaatgag attggtcgga atcaatgcca tcatgaccaa gccaagcaag
      421 gtaacagatg aaatagccaa catgatattt cgacgcattt cttttgtctg acgggaaaga
      481 acagctggga taatcataga catgggaata gctgtcatat tgaagaagcc agccattagc
      541 cctgcttctg ccttactaaa gccaactgat tgagaaatag ttggcaacca ggtaatctct
      601 gtatagtaga ggactgattg tagcccacca aatatcaaaa aggcaatcgc tgctttattt
      661 ttccagatag atgatttctg gtttccttgg ttctcacttg caaatcgatg gttatttttt
      721 acattaggca accagattag gaaagccata aacaccaatc ctgtaattag tagaataaaa
      781 aattcccaag aactagagga tacaattgga acagcaatca tggatgcaac tgttgccgcc
      841 acccccatga gagtgatata aatcgtcgta tacagaccaa ttttctttgg aaaattagcc
      901 gcaaccaaac ttggtagcaa aacattaata aacgctattg tagcaccaac aagcattgta
      961 ccaatgtaaa gggctggcaa gttgagaact cgcattcctg aacccagcac cataaccagt
     1021 aagactaggg ccattagttt ttccatgcca aacttagccg caagacgcgg tgctaaggat
     1081 gaacagagag caaacatgat gagcggaata gaggttaaaa ttcctaatga actcacttct
     1141 actcccagtc ctgctgccac atctgtcaaa atagcaggca aagctgtaaa gggggcacgc
     1201 atcacgatcc ctagcatgac gattcctgca attataaagg gtgattgttt tttcattttt
     1261 ctcctaagtc tcctaatgtt cggatttcag cactttcgat tataccatag ttgacaaatg
     1321 atagaaagtt atttccgtct attttttcta gttatagttt ttaactatgg atttgccatg
     1381 aaaataacta tttttcaaat gatagcttat gagttagaat aggttcatca ctaaaacgaa
     1441 agaaagagag atacatatat gacaacttca agattccaat tagtcggttc acttcttcga
     1501 ccagcaaact taggagaatt taaacgacaa atcgaagctc gtgaagacat caaataccca
     1561 ttctatgatg acttcgatgg ttacaaagaa actgaagctt cactcatcca aaaaattgtt
     1621 gcagaacaaa aagaaaacgg tttggatatt gtgacagatg gagaatttgg tcgttcaatg
     1681 tggcacctag attttctctg gggcttcgat ggtatcgagc gttacattgc tgagcatggc
     1741 tatccattta aagaccatga tggacagatt tttgaaacac gtaaggatat tggattgcgt
     1801 attactgctc cactttctgc aaaaaatcac catttcattg atattttcaa acaagtgaaa
     1861 gaattagcag gagaaacggt aaccaaacag actattttcg gtccagccca tgcctttaat
     1921 gaattgacaa ttttcaatgg acaagttgga ccaaatcagg tctacaagac tcgtgatgaa
     1981 ttaaaagccg ggctcatcgc tgtctataag gaatttttag accagtacaa ggaagctggt
     2041 ggacaaatcg ttcaatttga tgactgtctt tgggaattat tcgaccctgc taaccctgta
     2101 cctttcttac cacaagacga ttctgaagca ttggctgctt tggctgacga gtttgttgct
     2161 atcaataatg ctgttattga ttacgggcat gaaattggct tgaccgtctg gacgcacaac
     2221 tgccgtggta actatgaaag tcgctctgca gctggcggaa cttacgaagc tattgctgaa
     2281 aaattcctgc gtgaccaaaa atacgatcga ttcttccttg agtgggatga tgaacgtgca
     2341 ggtgatttaa aagctttaga aagcttgcgt gacaagaatg tcgaagtcgt tcttggtcta
     2401 ctttcaagta aaacttctga ttgggatgat gaagaacgtg tttacaaatt actcgaggaa
     2461 gcaagcaaga ttattccaaa agagcgtctc tacttgtcac atcaatgtgg attcgcttca
     2521 tgtgattcag gtaatgaatt ggccatcccg caacaatggg caaaaattaa acaaggtcaa
     2581 gaaattgcag agaagttttg gtcttaagcc acttcttgtt attcgtgcat aaaagcccgc
     2641 catttggcag ggctttttag actggtcgag ttctttccca ctcccactca tcaaggctct
     2701 tgacaacgga taattttgat tttcgaagag tataagcttg cataaacata caaaaaacta
     2761 ggccttaacc tagtccatga tatatatcgt atacttcttg ttttttgagt tggtagcgtt
     2821 tggccacttc tttgatagcc tgattaggtt ttatgccact tgcaatttcc ctttctacct
     2881 cagccttcaa gtccacttcg tcagcagaag gatagacttc ctcaccagca ccctcgacaa
     2941 taatcaagca ttccccctta agtggatttt cttccaaata ctctagtaat tcggaaatgc
     3001 ttcctctctg gtattcttcg tataacttgg tcaattctcg aacaaccgtt acctgacgat
     3061 caccataaac tgtcaacata ttttctaagg tatctgccac acgatatgga gattcataga
     3121 aaatttgcgt ctcaggatag gtcttttttt cttggaaaat ttctttttgc tgccccgatt
     3181 ttctcggtaa aaatccataa aaaatatgtg gctggggtgc caaaccagat gcaatcaagg
     3241 ctgtaattcc cgccgatgct cctggaagtg ctacaacagg aatatcttcc gcaatcgctg
     3301 cccctaccag gtcatgacca ggatccgaaa tggaaggcaa gcctgcatca gaaacttgcg
     3361 caatggactg accagatttg agccaatcaa tcaaaactgg aattttttca tgggcattgt
     3421 gttcatgaaa ggatatttgc ttggtttcta cttcaaaatg tttgagtagg aggcctgtat
     3481 ttctcgtatc ttccgctgca attatatcca cctctttcaa gatctgaaca gctcggaatg
     3541 tcatatcctg taagtttcca ataggcgttg gcaccagata taacgtccca aaactcgtct
     3601 gtcccttaaa tgatttttgt actttcatcc taatctcgat tcaataattc tacacagaac
     3661 atacaaggtt catcattatc cctacgttga ccataagaga aggtacaaat atggaaacca
     3721 tcttcgtaaa tattctccaa attctcctta ccaaagttgg aagatttatg gcctgtctta
     3781 tcttcttttt ccaaacgttc gcgcaacttg gaattttcca agcggagggt cgtattttca
     3841 tcaataactc cctgtaaatg ctttttgata gcatccactt ctgccaacgt ggttagaaga
     3901 ttctgcgaaa aatcatctag ggcatcaaaa atttcttttt tatccattat ctccctttct
     3961 tttattttac agagttatat attctagact attttgtaga cttacgtttg cctgccacat
     4021 cttcttagcc ttaagaacag catccatttt ttcacgtacg tttgccgatt ccatcatttt
     4081 ctcagataat atcagactca gaaggtcaaa caaacgtccc tgctcagctt tttccacagc
     4141 taaagacact aggattccaa cttgcaaata agcacgtgta ggctccacca agagcaagtc
     4201 tgtaaatttc tttgtctgtc ccatcaaatc taaaaagttt ttattttctg ctaacttttt
     4261 cgcttcttct tgacttgaaa ccaattgagc taagagattc gcttgtgttt ttaacaagcc
     4321 ctcctcttct aacattcgct ccatagctgg aatattttta ggaaaaccta taatctgtgt
     4381 ccgactcttg atagtaggaa ggacagcttc ttcttgattg gtcaataaga agatgtgaat
     4441 ggctgactga ggttcttcaa tcacttttag caaagagttg gctgcgttag catgcatttt
     4501 ctcagcatca cgaataatga aaacctgctt agtggattca aatccagatt gtgaaaaatt
     4561 cttaactaat tcacgtatcg tatctgtttt gataacattt ccctgcggtg caataacagt
     4621 aagatctgaa aactcatttg cttcaattag acgacaagac cgacacttct gacaaggaag
     4681 gacgccttct ttctcctgac aaaataacga ttgactgaga aaaattgcca tatcaaaact
     4741 agcaaagtca cctgaaaata gataggcatg tgccaaacgt ctctgctcta aaattgtcac
     4801 aaaacgttga aatacattgg gttgtaagtt tctaagttct tcaatcttca tattctttct
     4861 gcaaaccgtt tcttaatgac cgccagagca tctgcaacaa cagcctctag tggcttactg
     4921 gcatcaatct ttacaaaccg ccctggttcc tgctctaaaa tattaagata gccttgacgc
     4981 acccgtctat gcatatctgc tttttccata tccaagcgat ccacatcacg gtcagcattg
     5041 cgggcaattc gagccagccc ctcttctgta tcaatatcaa aatagagggt taaatctggc
     5101 tttaaaccat ctgttgcaaa ctggttgagc caatgaatgt catctatatc caaaccccgt
     5161 ccaaatcctt gataggcaat agaggaatcg ataaaacgat caatcaatag caatttcccc
     5221 tgtgccaagg gtggaagaat cttctctttc aaatgctgac gtcgtgctgc aatgaacaat
     5281 aacagctccg tcttatcatc catcgctgta tttgcaggat tgagaattat attgcgaatt
     5341 tcttctgcaa tagccacccc acctggctct ctagttgtga ctacttctgg ccccaattcc
     5401 tgcaaggctg gcaataactg ttgcaatacc gtcgttttcc ctgccccatc tggaccttca
     5461 aatgtaataa aaaaaccgtt tctcatcttt ccctcgctct tagtaccagt atgcaccgtt
     5521 cagcacttca cttgtgaaga ctggttctta gatattcatt ccattttacc aaaaattagt
     5581 caaaaaatca cttgaaaatt gacttgtttt taaggagaaa atcttttaaa attgatatat
     5641 acacatattt ggagatgctt atggttcgtt tgaaaaatat tttacttatt ttgcttggtg
     5701 cagggctatt tgcctttggg ctaaattatt taatcatgcc caatcgtcta ttcgagggtg
     5761 gcgcaactgg attgacgctg attatttact acctttttca tattcaacct tggataatga
     5821 acattgtgat taatattcca ttgttcatcc tcgggtggaa aatactcgga aagaagactc
     5881 tctatctcag tatcttagga acctttagcg ttaccttttg gttggccatt tttgaaaaaa
     5941 tccacttttc aatcaatcta caacaagatt tgatactcgt tagtatcctt ggtgggattc
     6001 ttatgggact aggattagga accatcttcc gtgctggcgg aacgactggg ggaagcgata
     6061 ttattgctcg tataggtcat aaattcctac cttactccat tggagaaatc attcttgcag
     6121 ttgacatcct catcctaact ctgattgtta ttgtttttaa agacctccga acagttttat
     6181 atactttaat gatggttgct attgcttcca aggttattga ttttgtcacg gagggcggat
     6241 atggtagcaa gggtgttatg attgtttctc aaaaatctga ccaactggct caggctattg
     6301 attgtgaaat cgagcgtggt gtcaccttta tcaaagcaca aggtttctac agcaaagccg
     6361 atgtcaacat gatttactcg gtcatatata agagtcaact ccaagagatg aaggagctta
     6421 ttcatcgtat tgacccccac gcatttatca ccattacaga cgcacatgaa gtactcggtg
     6481 agggatttac tttagatcga gataaaaaac caatagaacg ccattaaaca aacctgccgt
     6541 ttcgctgaac tgcaggtttt atattattga ttggtttcaa gtggttgctc tgcctcaaac
     6601 tgtgcttctt tgccttgctc cagcaaagca gcaatgattt tttgatcgcg tagcttaaca
     6661 gaatcattga tagtctctgc tgaccgtaca atagctactt ccaactcccg acgtttctga
     6721 cgcccttcat ctatagctgc aataatgcca ttattttggg caaccaaact ttctgccaat
     6781 ttggtaacag atgctactga aagggtcgga ttctgagctg tgcgttccat catcgggatg
     6841 gcttcttttg atgtatccgc aagcatttga agggcagcat tatttgcatt gacaatcgca
     6901 tctgccgtct ggctagactt gatagattgt tgtaagatgc ccaactgtgc aatggacaat
     6961 ttcattgttg gaatagtatt gcgacgtaac atcccaaggc gctgtttcat atccgaagaa
     7021 accttgacca gattacgcat ctgcggtgtc gttgtccacg caacatacaa gcgactcata
     7081 tactcagaat gttgttgttc gaggatatta gcaacctctg ttacacgtgc taacttctca
     7141 gaagctactt gataatcgac tgtagtcggt actaaagttg cgaggttttc ttgtgccaat
     7201 ttcgcacgct ctgccgcttc ttgaccagtc gcttcaataa aggcgatgac ccccaccaaa
     7261 ttctcgatag acttggtatt attttcaatc aaaagctctg cagaaacaat attgcgcgcc
     7321 aacacctctt cctgcttgac cacactggct gccatgctat ccattttctt ttcaatggtc
     7381 tgcgaatcga aataaaattc ctgtaaatca tttttggtct gcttgaacag gcgttggaag
     7441 aaacctggtt tcttttctaa ttcagcagtt gtggaaatgt ccttatactt agccacaaaa
     7501 ccattcaatt cacggttggt attggccaaa agctcatcca cctgtggaat tttaatcttc
     7561 ttctgttctg acaaaatgtg attgaccgtg gcattgactt cttctaccgc ttccttacca
     7621 aaatccaaga gggcattttg attagctaag aaattatcca ccaattgggg agccttggca
     7681 cgaatacctg tttgttgttc cagtgtcaac tgttccaaaa aagacacttt tgaattatct
     7741 ccagtcggag tcgcttgaat aagctctgtt gtcttatctt tggttgataa actattatta
     7801 gcaatctgat caatatcgaa attaaatcct gacatcttct ctattcctct tctcttttca
     7861 tcatacgcag actgacttca aaatctttca tatccgcttc gttaaattgt ttgatggtat
     7921 catccaaatc cttatcaaac atttccatgg ctgattttgc ctgcaccaat cgttcttctg
     7981 cgttaaaata atctttcggt gattttttga ttttcaaata accctgtaaa atatcgttaa
     8041 accgattcat attggcctca tggatagctg tcaactcttc ccgcttatct gtcgcctttt
     8101 ccagcttttc aagaataaca agattgtctc tttgaatatt acaataggtt tctagaattt
     8161 caggagctaa atccgctatt ataccttgtt catccatcac agcattacca ccagtagaaa
     8221 cttgtgtgtc agtatttctt tctattttag cttctggcag ttttaaatga tattttagtt
     8281 gcaaggtatc tacaacttct ttactttcat atccattcag atattttcca actttttgat
     8341 agagctgatc taattcaaat aattgtccta acagtctgtt tctcaactgt aacacaccct
     8401 tttcctcacc cttttccacc aagagctctt gatgcttttc atagcccgaa cgaatagata
     8461 gcgccaatac ctgcaagcgt tttatagaat ctctattcaa aaaacgctgg atagcctctc
     8521 tcttgtaaaa atagccaatt aagagcccaa tgggcaataa gatataccag aaactcatga
     8581 caaaaccgat tgctaataaa acaaggaggg ccacaagtcc tgaattattt ttaccatatt
     8641 tcttatactt tttatatttg tgccccatag agcctcctca cacatgatat actttattat
     8701 atcacattcc cct
//
LOCUS       DATUCG010000102         8664 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.104, whole genome shotgun sequence.
ACCESSION   DATUCG010000102 DATUCG010000000
VERSION     DATUCG010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8664
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.104"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(<1..618)
                     /locus_tag="VCA20_001827"
     CDS             complement(<1..618)
                     /locus_tag="VCA20_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEP1835599.1"
                     /translation="MDYTDISILHTNDMHSYMENFPKKAQLIADIRARNEQKGIPTFV
                     FDSGDLFSGNIFFNMYRGVKEIELMNQIGCQAMTLGNHEFDHGDELLSRLDDYAQFPI
                     VSSNLRYQDVEAADELVPLEDVLEFDMNGKNLYVLGLTTLETQEVASPSENVIFEDHR
                     QALRRLVDQILAKDSQAHLVLLSHLGYDEDLALAKDFPEVNVILGG"
     gene            complement(848..3019)
                     /gene="nrdD"
                     /locus_tag="VCA20_001828"
     CDS             complement(848..3019)
                     /gene="nrdD"
                     /locus_tag="VCA20_001828"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEP1835600.1"
                     /translation="MNVQENVLPSIELLVLKRDGRTVSFDQDKIFSALRRANQELEHP
                     VSEAGLKIVLEAVLAEIGRRFEKDVQIYEIQTVVEQELLKAHLYDLAELYIQYRTRRD
                     FRRHQATDINFEIHKLLSKDQSVVNENANKDADVFNTQRDLTAGIVGKSIGLKLLPAH
                     VANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLAQGFKIGNADVESPKSIQTATAQ
                     ISQIIANVASSQYGGCSADRIDEVLAPYAELNYQKHLKDAKDWVLPDKQEEYARAKTQ
                     KDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSWFEREIQKAILNIRIKGLGREG
                     RTAIFPKLIFTVKRGLNLEPDSPNYDIKQLAIECATKRMYPDMLSYDKIVELTGSFKV
                     PMGCRSFLQGWKDENGQDVTSGRMNLGVVTVNLPRIAMESAGDKDKFWEIFAERMAIA
                     KDALVYRVERVKEATPANAPILYQYGAFGKRLAKTDAVDEVFKNRRATVSLGYIGLYE
                     VATVFYGGEWEINPEAKDFTVDIIKKMKALVEDWSDEYGYHFSVYSTPSESLTDRFCR
                     MDTEKFGIVKDITDKEYYTNSFHYDVRKNPTPFEKLDFEKIYPAVGASGGFIHYCEYP
                     VLQQNPKALEAVWDYAYDRVGYLGTNTPIDHCYACDFEGDFEPTERGFKCPNCGNSDP
                     KSVDVVKRTCGYLGNPQARPMVKGRHKEISARVKHMNGSSL"
     gene            complement(3163..3336)
                     /locus_tag="VCA20_001829"
     CDS             complement(3163..3336)
                     /locus_tag="VCA20_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835601.1"
                     /translation="MKIILTIVYSLGVVWNILFLAVNVGRIPVINLLWPAVILVACAI
                     NLYFLWTRKQESE"
     gene            complement(3370..3810)
                     /locus_tag="VCA20_001830"
     CDS             complement(3370..3810)
                     /locus_tag="VCA20_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HEP1835602.1"
                     /translation="MKKYLLSASLGIAIGTIISIIASAVFGQGIYLPLNPFSTMGAYY
                     VEHFNQPTVMLICVAIWAAIGILFQLADKIFEQDWSLLRMVATHFCITALGFTPLGIL
                     AGWFPVKLVALLFFWFIFVLIYASLFFLNYRKMERQIKDINGRL"
     gene            complement(3807..4250)
                     /locus_tag="VCA20_001831"
     CDS             complement(3807..4250)
                     /locus_tag="VCA20_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator"
                     /protein_id="HEP1835603.1"
                     /translation="MKVKLAISSDIVEDLVTIEAQAMSEQVSHLVSYIQNLDKQRSSL
                     TVKKGEQVYLVEHDEIVRLYLEDKVLQVETVETTYTSSLRLYQVKEDLPANFLQISQS
                     EIIHIKQLDHLKLTANGLVKLVMKNGSVTYSSRRYLKVIKERLGL"
     gene            4451..4924
                     /locus_tag="VCA20_001832"
     CDS             4451..4924
                     /locus_tag="VCA20_001832"
                     /inference="COORDINATES: protein motif:HMM:NF024999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEP1835604.1"
                     /translation="MTLALCQACSFGLIDSFPCALCQPNHNLSASLLSEVADISYNAT
                     KKVRYYGTKFSVLINDRGFHIDCVVTPASVSDGMVAFELLENSPFSVIYGDKGYVDKQ
                     AKSHLSQSGIQPISQLRKNMVDYAWFDNYRHSVLGQRRHCLTILLCLKAVIMDLV"
     gene            complement(5038..5550)
                     /locus_tag="VCA20_001833"
     CDS             complement(5038..5550)
                     /locus_tag="VCA20_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter accessory protein"
                     /protein_id="HEP1835605.1"
                     /translation="MKQRLIILTLLTALAGWGLTIFAFHQANTSVYDKASPEFLQVDT
                     STTIENIKSNKAGLYYFGFESCPWCQDLLPQLQEELRNSSSKAYLVNTKSKAFTPEAK
                     KIVTSIYKEHLGGDKLYVPFLVAINRKGQVRVHMGTVKGHDASSEEMSSEQLKELKNI
                     LSSLNEFSRS"
     gene            complement(6154..7518)
                     /locus_tag="VCA20_001834"
     CDS             complement(6154..7518)
                     /locus_tag="VCA20_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835606.1"
                     /translation="MLKSKTFKNILFLVNIYIKDSLKGLAQHSLFKTKRNRWIITMSS
                     IVGYLFYFYLNVVEFAKLSKLGQNDSFDELKSTISISIASYNNLAVIAGILIFLLINA
                     TVHLQSSSLFLSKVLPYNEKEVFLSVKLFKLGLGTLCFELFFIILIPGLGILRSPLIA
                     ILLFLSCHLIFWASYLFANFLYILSLRHLPLSDKHIHNLIYVSYFLVGLSYLFLFRFK
                     VEFFLAQAIDSPQLLSLLILIGSMLAMSLLLRFDRVHYDVVFLQPRFTLLPFTIFNSL
                     LKGVPLAIIRTKLFLYSFLFIVFIGVYSTFVANLEVAAYNLLDFWPMLGLAFLNYADS
                     TLDHRKLYRHLRIDTRSEVIRLVLTAVLIHLPSLLFACYVQENYLVFLSGISLSITSL
                     MVGFLFPRSVSSSNETLAFMLLLLAVPIVYLLIKVPILLVPTILVLILTLSYIIKKET
                     EVHL"
     gene            complement(7463..8191)
                     /locus_tag="VCA20_001835"
     CDS             complement(7463..8191)
                     /locus_tag="VCA20_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1835607.1"
                     /translation="MLTIDYLYKKFGQQQVIINLNLQIEKQGVHIIVGTNGSGKTTLM
                     NTLTNIIKADSGSITCNGHSVESKQYKESIFYIPSDFYLPEYLTATEYLDFMLPHYPK
                     SDNKQLDILLDIFDLGDHRNKLLETFSFGMKKKIQLIAAIASNVDYVIADELFGGLDF
                     DTVLLVQELLQVLQKERSFIIVSHDLNTLLAFPDNIYIMANGQVNFFRDKVENINKYI
                     KEEGNLSAKIQNIQEYFVPSQYLH"
     gene            complement(8248..8469)
                     /locus_tag="VCA20_001836"
     CDS             complement(8248..8469)
                     /locus_tag="VCA20_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835608.1"
                     /translation="MSAVSQSLQLYLMNLELKYQKMIENRWVWFVFGLVLLGAIGLYA
                     WYCTSRGRSFAFNVKFNWPRSGQMGIACN"
BASE COUNT         2682 a         1782 c         1606 g         2594 t
ORIGIN      
        1 tccacccaag atgacattga cttctggaaa gtccttggcc aaggcaagat cttcatcgta
       61 tcctagatga ctgagcaaga ccagatgagc ctgtgaatcc ttggcaagaa tctgatccac
      121 caatcgccgc agagcttgtc tatggtcttc aaaaatcaca ttctccgacg gtgacgcaac
      181 ctcctgggtt tccaaggtag tcaaaccgag gacatagagg tttttcccat tcatatcaaa
      241 ttcaaggaca tcctcaagcg gcaccaattc atcagccgcc tccacatcct gatagcggag
      301 attggaggac acaattggaa actgagcata gtcatccaag cgactgagca actcatctcc
      361 gtggtcaaac tcatgattgc ctaaggtcat agcctgacaa ccaatttgat tcattagctc
      421 gatttccttg actcctcggt acatattgaa gaaaatattt cctgaaaaca aatccccgct
      481 atcaaagaca aaggtcggaa tacctttttg ctcattccta gcccgaatat ccgcaatcag
      541 ttgcgccttc tttggaaaat tctccatgta ggaatgcata tcattggtat ggagaatact
      601 aatatctgta tagtccatac tattttttct tgttttttgc ttgtttgagc aattcctgca
      661 cacgcgcttt tttatcctgc ttcacattag aattcttctc atgatagcgt gtcaccaggg
      721 ccttcccctt atcgtctaat tgatattttc ccattgtttt ctcctctttt ttatctctta
      781 tttgtttata ataaacttcc acagctaact ggaactgtgg aagctctaca tagtctctgt
      841 ttattactta taagctcgac ccattcatgt gtttcacacg cgccgaaatt tccttgtgac
      901 ggccttttac catcggacgg gcctgtggat tgcctagata accacaagtt cgtttaacaa
      961 catccaccga cttaggatcg ctattcccgc aatttggaca cttgaagcca cgctccgtcg
     1021 gctcaaaatc accctcaaaa tcacaagcgt aacaatggtc aatcggagta ttggtcccca
     1081 gatagcccac acggtcatag gcatagtccc aaacagcttc caaggccttt ggattttgtt
     1141 gcagcacagg gtattcacag taatggataa atccaccact ggcacctaca gcaggataaa
     1201 ttttctcaaa gtccagtttt tcaaatggtg ttggattttt gcgtacatca taatggaaac
     1261 tgttagtgta atattcttta tctgtaatgt ccttaacgat gccaaatttc tctgtatcca
     1321 tacggcagaa gcggtctgtc aggctttcag atggtgtcga ataaactgag aaatgataac
     1381 catattcatc cgaccaatct tcaaccaagg ctttcatttt cttgataata tccaccgtga
     1441 aatcttttgc ctctgggttg atctcccatt cgccaccgta aaatactgtt gcaacttcat
     1501 acaaaccaat gtaccccaaa gatacggttg ctcgacgatt tttaaagact tcatctactg
     1561 cgtctgtctt agccaagcgt ttgccgaatg ccccatactg atacaaaatt ggtgcattgg
     1621 caggtgtcgc ctccttgacc cgctccacgc ggtaaaccaa ggcatccttg gcaatggcca
     1681 tacgttctgc aaagatttcc cagaacttat ccttatctcc agcagactcc atggcaatcc
     1741 gtggcaaatt gacggttacg acacccaagt tcatacgacc agatgtcaca tcctgcccat
     1801 tttcatcctt ccaaccttga aggaaagagc ggcagcccat tggcaccttg aaggaacctg
     1861 tcaactcaac aatcttgtca taagaaagca tatctggata catacgcttg gtcgcacatt
     1921 caatcgccaa ttgcttgatg tcatagtttg gagaatctgg ctccaagttc aatccacgct
     1981 taacggtgaa aatcaactta ggaaagatgg ctgtccgacc ttcgcgccct agacccttga
     2041 tacgaatatt caaaatcgct ttttgaattt ctcgctcaaa ccacgatgtc cctagcccaa
     2101 aaccgagtga ggtaaagggt gtttgtccat tggaagtaaa aagggtgttg atttcatact
     2161 ccaaagactg catagcatca tagatgtctt tttgcgtttt agcacgcgca tattcttctt
     2221 gcttgtctgg caacacccaa tccttggcgt ctttcaaatg cttttggtaa ttgagttccg
     2281 catagggcgc caaaacttca tcgatacggt cagctgagca accaccatat tgactagagg
     2341 ccacattggc aatgatttgt gaaatctgag ccgtagctgt ttgaattgat tttggacttt
     2401 ctacatccgc atttcctatc ttgaatccct gtgccaacat ccctttaaag tcaatcaaac
     2461 aacagttggt cattggtgta taagggctgt aatccaagtc gtggtaatgg atatcccctt
     2521 tttgatgggc attggccaca tgagctggca ataatttgag accgatagac tttccgacaa
     2581 tacctgctgt caggtcccgt tgagtattga acacatccgc gtccttattg gcattttcat
     2641 tgaccacaga ctggtccttg ctcaacagct tatgaatctc aaaattaata tcggtagcct
     2701 gatgacggcg aaaatcacgg cgtgttcggt attgaatata aagctccgcc aagtcatata
     2761 aatgagcctt taacaattcc tgttcaacga ccgtttgaat ttcgtaaatt tgaacatctt
     2821 tctcaaatcg gcgaccaatt tcagccaaaa cagcttctaa tacaattttt aaacctgcct
     2881 ctgaaacagg atgttctaat tcttggtttg cccgccgaag agcagaaaaa atcttatcct
     2941 ggtcaaagga tactgtccgt ccatcacgtt tcaaaaccaa taattctata ctcggtaaga
     3001 cattttcttg cacattcatc tgacaaacct ccaaaattct tcacttctat tagtgtagca
     3061 cttcacaaaa caaaaatcaa tatcttgtgt aaaaaaatat aaaaatagaa aaattacact
     3121 aaatatagac ttcgtttctg aaaaacaaat gaaaacaaga gcctattctg actcttgttt
     3181 tctagtccat aaaaagtaca ggttaatagc acaggccact aaaatcacag caggccaaag
     3241 caagttgatg acaggaattc tgccaacatt caccgcaaga aagaggatat tccacaccac
     3301 tcccaagcta tagacaatcg tcaaaataat tttcataaga cactccaatt aaatgatagt
     3361 gctattatac tacaaacgac cattgatgtc ctttatctgc cgctccattt tccgataatt
     3421 taggaaaaag agagaagcgt aaatcaagac aaagataaac cagaaaaaga gaagagccac
     3481 aagtttcacg ggaaaccagc cagccaaaat gcccagcggt gtgaagccta aagctgtgat
     3541 gcaaaaatgc gttgctacca ttcgtagaag gctccaatcc tgttcaaaaa tcttgtctgc
     3601 cagctgaaat aagatgccga ttgccgccca aatggctaca caaatcaaca tgactgtagg
     3661 ctgattaaaa tgttctacat agtaagcacc catggttgaa aagggattga gagggagata
     3721 gattccttgc ccgaaaacag ctgatgcgat aatggagata atggttccga tggcaattcc
     3781 taaactggct gataagaggt attttttcat agtcctaacc tttctttgat aacttttaga
     3841 tagcgacggg atgagtaggt gaccgagcca ttcttcatga ccagtttgac cagaccgttg
     3901 gcagttagtt tgaggtggtc cagttgcttg atgtgaatga tttccgactg agagatttgt
     3961 aaaaagtttg ccggcaagtc ctccttaacc tgatacaagc gtaggctgga agtataggta
     4021 gtttctactg tttccacctg taaaacctta tcttccaagt agagtctgac aatctcatca
     4081 tgctccacaa gataaacctg ttcccctttt ttgacagtta gactggatct ctgcttatcc
     4141 aagttttgaa tataggatac cagatgacta acctgctctg acatagcctg agcttcaata
     4201 gttactaagt cttctacaat atctgatgaa atagctagtt ttactttcat cttctctcct
     4261 ttctctttct gaccctatta aacaccattt ctttactggt ttcaagggct tttgactatg
     4321 tggtcgtttt ccagcactaa acggcaaaaa aagcagaaca gctgtcctga cttagtcatt
     4381 ctttagaaat gttagccccg tcactatctg ccaaaatttt gccaaaagca ttcaacgtgt
     4441 gtgctatttt atgaccttag ctctgtgtca agcttgctct ttcggcttga ttgacagttt
     4501 tccttgtgct ttgtgccagc ccaatcacaa cctaagtgca tcattattat cagaagtggc
     4561 agatattagc tacaacgcta ctaagaaggt acgctactac ggaaccaagt tttcggtact
     4621 tataaatgat cgtggttttc atatagattg tgtcgttacc ccagcctctg tttcagatgg
     4681 catggttgct tttgaattgc ttgagaatag ccctttctct gtcatttatg gagataaggg
     4741 gtatgtagat aagcaagcga aatcacattt atctcaatct ggcattcagc cgatatctca
     4801 actacggaaa aacatggttg attatgcatg gtttgataat tacagacact cagttttagg
     4861 acagaggcga cattgcttaa ctattctctt gtgcttgaaa gcagtcataa tggatttggt
     4921 gtaactttga aatatttcct tatttatgct tgacgaacta cctcaacggg taaatatagt
     4981 gtctgcctta ttcatcgtgg agataatcaa ggtatagata ttaagctact tttattctta
     5041 agaacggcta aattcgttta aactactcag gatattttta agttccttca gctgttcaga
     5101 ggacatttct tcgctactcg catcatgtcc tttgacagtt cccatatgca ctcgtacctg
     5161 tcccttacga ttaatggcta ctaggaaagg aacatacaat ttgtcaccgc ctaaatgttc
     5221 tttataaatg gatgttacaa tttttttagc ttcaggtgta aaagctttag attttgtatt
     5281 taccaaatac gctttgctgg agctattgcg caattcttcc tgtaactgtg ggagaagatc
     5341 ctgacaccaa ggacaacttt caaagccaaa ataatacagc ccagctttgt tacttttgat
     5401 attttctata gtggtcgatg tatccacctg tagaaattct ggactagcct tatcatagac
     5461 agaggtattt gcttgatgaa aggcaaatat agttagaccc catcctgcta atgctgtaag
     5521 taaagtaaga ataattaatc tttgtttcat attttcctca caaaaatagt atatagtgaa
     5581 aggagaatga tttctccatc atttgtaata ggcatcaagt tgaagatatt cgataagaaa
     5641 cagattttca atgagaagaa aaagagagat tctccctgca tgaataaacc gactaagaat
     5701 agtaataagg gtgcggagca ggcaatgcaa agattatgcc actgattgtt tttattttca
     5761 tattggacag agggggcgaa caatttccaa tttattctcg gtcgataccc caatccaact
     5821 gctgtcaacc agtggatccc ttcgtggata aaaatagata aggccagact aagcatgata
     5881 gctatgcacc aaatcatggg ttcaccacat atttttttcc aagagaatag atagctaacc
     5941 aaacgaaaac aaaaccaaaa taaacaaggt aaaaccacgg agtgatcgaa aagtagtttg
     6001 ttagcgtctc gctcgtgtcg atgttttgaa tttgtaaccc catcaacgta atcttagtgg
     6061 cattatatag ttgaaaaagc tccgttgcag aattgatgaa aaaagtaata agccaagcta
     6121 tactagcata tgcccattgt tgtagatagt ttctcataaa tgaacctctg tttctttttt
     6181 tatgatgtaa gatagtgtta gaataagtac caatatggtt ggtactagca atattggtac
     6241 ctttatcaat agatagacaa ttggaacggc aagaagtaag agcatgaaag caagcgtttc
     6301 attcgaacta cttactgatc taggaaataa aaaaccgacc attaagctag tgatgctgag
     6361 cgatattccg ctaagaaata ctagatagtt ttcttgaacg tagcaagcaa aaagtaggct
     6421 agggagatgg ataaggacgg cagtaagaac caaccgaata acttcacttc tcgtatcaat
     6481 tctcaaatgt ctatagagtt ttctgtggtc tagggtagaa tcagcataat tcaggaatgc
     6541 taggccaagc ataggccaaa agtccaataa attgtaagca gcgacttcta ggttggctac
     6601 gaatgtggaa taaactccaa taaagacgat aaatagaaag ctatatagga aaagctttgt
     6661 acgaataata gccagaggaa ctccttttag gagactgtta aaaatagtga acggaagcaa
     6721 ggtgaatcgt ggttgtaaaa aaacaacatc atagtgaact cgatcaaatc gtaacaacag
     6781 ggacatggct agcatgctcc caattaaaat aagcaatgat aagagttggg gagagtcgat
     6841 agcctgtgct aggaagaatt cgactttaaa tcgaaataag aatagatagc tgagccccac
     6901 taagaagtaa ctaacataga taagattatg tatgtgtttg tcgctcaaag ggaggtgccg
     6961 tagtgatagt atatatagga aatttgcaaa gagataactg gcccaaaata tcaagtgaca
     7021 ggataaaaaa agcaatatcg ctatcagcgg tgagcgcaat atccccagtc caggaattag
     7081 aataataaaa aatagttcaa aacaaagagt tcccagtcct aacttaaata attttacaga
     7141 tagaaagacc tctttttcat tatacgggag cactttggat aaaaacaagg aactagattg
     7201 taagtgaacc gttgcattta ttaaaagaaa tataaggatt ccagcaataa cagccaagtt
     7261 attatagctt gctatggaga tagaaatagt cgattttaat tcatcaaagg agtcgttttg
     7321 ccccaatttt gacagtttgg caaattctac gacattcaaa taaaaataga ataaatatcc
     7381 aacaatgctg gacatcgtaa taatccagcg gtttcttttt gttttgaata aggaatgctg
     7441 tgccaatcct tttaaggagt ctttaatgta gatattgact aggaacaaaa tattcttgaa
     7501 tgttttggat tttagcactc aggtttccct cctccttgat gtatttattg atattttcta
     7561 ctttatctct gaaaaagttg acttgtccgt tggccataat gtagatatta tctggaaatg
     7621 ctagaagagt gtttaagtca tgagaaacaa taataaaact tcgctctttc tgcaatacct
     7681 gaagtaactc ttggactaat aagacggtat caaaatcaag ccctccaaac aattcatctg
     7741 caatgacgta atctacattt gaagcaatgg ctgctatcag ttgaattttt ttcttcatac
     7801 caaaggagaa ggtttcgagt agcttatttc gatggtcacc caaatcaaaa atgtctaata
     7861 aaatatccaa ttgtttgtta tctgactttg gatagtgagg tagcataaaa tctaaatatt
     7921 ctgtagccgt cagatattct ggtaaataaa aatcagaggg aatatagaaa atagattctt
     7981 tgtattgttt ggattcgacc gagtgaccgt tgcatgtaat gcttccgcta tctgccttta
     8041 taatattggt tagagtattc atcaaagttg tttttccact accatttgtc cctacaatga
     8101 tgtgaacacc ctgtttttca atttgtaaat tcaaatttat gatgacttgt tgttgtccaa
     8161 attttttgta taaataatct atcgttaaca tttagtacct caatcaatgg aagtagagag
     8221 ttttttgtct ctctacttct taaacaatta attacatgct atgcccattt ggcccgaacg
     8281 aggccaatta aatttcacat tgaaggcaaa acttcgacca cgactggtac aataccaagc
     8341 atataaacca attgctccca ataggaccaa accgaataca aaccacaccc atctgttttc
     8401 aatcattttc tgatatttca gttctagatt catgaggtag agctgtagtg attgtgatac
     8461 tgctgacata taatgtctcc tttaaaaata ttgaggaata tcctctcgta caatctattc
     8521 gcaaagtagt gtaaaaaatc aaaaaaattt gaaaaaatat ttttttcgtg ttagcttata
     8581 ctcactttat actcatttaa aatcaaaaat agccacctac cactcaaagt ggatatggag
     8641 cgttttgatt ttgttatttt ttga
//
LOCUS       DATUCG010000103         8647 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.105, whole genome shotgun sequence.
ACCESSION   DATUCG010000103 DATUCG010000000
VERSION     DATUCG010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8647
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.105"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            <1..230
                     /locus_tag="VCA20_001837"
     CDS             <1..230
                     /locus_tag="VCA20_001837"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835609.1"
                     /translation="VFFIDFENLSYTNIAFDIAALFETCNELNIANGYNLLEFLRLFK
                     REGLFFSVSDVLFILFEIFRFEFEGDYLWKS"
     gene            218..1162
                     /locus_tag="VCA20_001838"
     CDS             218..1162
                     /locus_tag="VCA20_001838"
                     /inference="COORDINATES: protein motif:HMM:NF012235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEP1835610.1"
                     /translation="MEIVRLESVSHAYQVDKTAFKSFFKRESTQKYVLKDVSLTLNQG
                     DCVSLIGLNGAGKSTLIKIMTGLIRPTNGTAYLFGQDSFLCRKQNVREIGVVFGQKTQ
                     LWWDLSLLESFEMLCRIYGNNPKNNQWLDYLIDLMEVRDFCSRPIRELSFGQRMRGEF
                     IACFLHKPKIVFLDEPTVGLDIVSKRIIVHFLREINQTEGTTILLASHELDDVEKITR
                     QMYLIDGQRLKYHGAISDFKEGRKLRKVIIPSFLAQDEAREFAVSIQQNASMTEMLIE
                     EEKLDLLQEFVKDQKIRDYHITDASLADILLLDKETSR"
     gene            1159..1935
                     /locus_tag="VCA20_001839"
     CDS             1159..1935
                     /locus_tag="VCA20_001839"
                     /inference="COORDINATES: protein motif:HMM:NF017946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEP1835611.1"
                     /translation="MNVLAYTIAEFRRRKSYRVSTILTILGAFLVVAIQISVWTSVVT
                     ENTSSQLVLYAVISRMMFILFPDHRTASIISEMIQKGDAAVYFLRPHSFMGMHFFRQV
                     GSFVYDLIFVVTPLMFALVFYSFVTGISLVPEMVLSFIMSALLGLLISFLFGYLLGLL
                     SIRFNQILGVLEFYDALLMLFGGSVLPISFFPEMLQKLIVLTPLYAMLSVPSSILSAL
                     LPAQYVLQQLCLQMFWVLLLALFISMYQKKLFKVMNYYGG"
     gene            1938..2723
                     /locus_tag="VCA20_001840"
     CDS             1938..2723
                     /locus_tag="VCA20_001840"
                     /inference="COORDINATES: protein motif:HMM:NF017946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEP1835612.1"
                     /translation="MTRYFYLYIHFMKLQWKRYIQYREDAVIGIIGYSVNTVISIYVL
                     KVFLDNLSNFPGWSTNELVLLFGFVTMARAGWNFIFINTLFIGDYVREGTFDVLLLRP
                     VNTLLLIIFSKIDFESIGEFCIGLIIFWMALLTEFETLSAWFILKIILLLIGSILCYA
                     AIHLFVNSLTFFIKDVYSINFIVWRLDETTRYPNSIYPGWLSKLLWFIPYAFIGYFPL
                     MSITKSLPLLADLGTISAGYIIFLLVYKIIWKFSISRYHSYRQ"
     gene            3385..3750
                     /locus_tag="VCA20_001841"
     CDS             3385..3750
                     /locus_tag="VCA20_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013851909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835613.1"
                     /translation="MTDHYRIIRKEITLTPGELKQIQELMKQEHADQFSPFVRQKLMN
                     LVERRQSVADWFALWQSQKIEQISRDILKVTTLVEHTQQVTAEHFHIILTCVQELMVE
                     VGKTIPLSSDFRDKYMGGQ"
     gene            3750..4115
                     /gene="mobC"
                     /locus_tag="VCA20_001842"
     CDS             3750..4115
                     /gene="mobC"
                     /locus_tag="VCA20_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HEP1835614.1"
                     /translation="MENRYRTNLKKVFLTDQELETLNNRIANSECQNFSVYARKVLLN
                     PNMTFLTIDTSNYQDLIFELRRIGNNINQIARAVNQTKLLTVEQRDHLRSGIGQLIIE
                     LDKDFNIKCQQLREFYGNH"
     gene            4102..5970
                     /locus_tag="VCA20_001843"
     CDS             4102..5970
                     /locus_tag="VCA20_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEP1835615.1"
                     /translation="MVITKHFAVHSNKYRKSLIRYILNPEKTNQLKLVSDFGMSNYLD
                     FPTYEELVEMYQANFVNNDRLYDSRNDRQQIKQTKIHAHHLIQSFSPDDNLTPEEINR
                     IGYETIKELTGGNFRFIVTTHTDKNHLHNHILINSIDLNSDKKLKWNYAQERNLRLIS
                     DCIAKEAGAKIITYNRYSHEKFVTYRKSNRKFELKQRLYFLMENSKSFDEILEKASAL
                     NLQIDFSRKHARFLMTDMEMKQVIRGNQLDKRRPYTEEYFREEFAKRAIKKRLDFLLP
                     RAQSLSHLLEMAEQINLVISLKKKYVTFHLTENDQTISLENKKISTKHLYDVAFFEDH
                     FEKRENTPELIRENLVTDFEDYCSEAEEEKIPYEKLWKAYEEFKEKRDQVQEFEVVLA
                     GHQIDKLVKDGLFIRVNYGVKKDGLVFISNRHLDIKDTEAGKSYHVFIRETAQFFIYH
                     KEDSMLNRYMRGRDLIRQLTNDSKTIPKRRRLKLETLKHKIDEINLLIELNIDNKSFQ
                     EVKDEIIKDIAQLDLAITNLQDHIAYLNRVAEVLINLNNSDPENRRLARYDYAKMNLT
                     AGATLSAVESELRTRQKELNNRIDNYEYQVKKLEKFVRIVNDSIDIKKEKVERFIE"
     gene            6062..6928
                     /locus_tag="VCA20_001844"
     CDS             6062..6928
                     /locus_tag="VCA20_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018373977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP1835616.1"
                     /translation="MVMLLASRIKSRRKELKLSQKELAEGICKQGQISRLENGEYTPG
                     SELLYQLAQKLNVSMDYFFDEQISDESTELIDFKKIAKNFVSQRNYESLKYIYELEKE
                     KAHRLSLSDKIYLEWIASLVDFYVYDRKETAIEKLEKVLTSLNKTDTNYLQISNTLFN
                     FYFETNNLSKFDFIKEELNLHLQVLTLNTIEELELSVKFSYNLSRYFWLQDDIESAIK
                     QVTATIKLCQDYRTTYLLADLFLLLGNLSENFSDKIMVRSYFEMAYFLYKNIDENKEM
                     ALTVEHYIAENF"
     gene            complement(7127..7552)
                     /locus_tag="VCA20_001845"
                     /pseudo
     CDS             complement(7127..7552)
                     /locus_tag="VCA20_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            8116..8253
                     /locus_tag="VCA20_001846"
     CDS             8116..8253
                     /locus_tag="VCA20_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835617.1"
                     /translation="MKQFLKKIIVASLLGGLLLAIGNSGINKPRPMGGIDPGPANTYV
                     K"
BASE COUNT         2866 a         1303 c         1672 g         2806 t
ORIGIN      
        1 aagttttttt tatagatttt gaaaatttat cttatacaaa tattgcgttt gatattgctg
       61 cactttttga gacatgtaat gagcttaata ttgcaaacgg ctataattta ctagaatttt
      121 taagactttt taaaagagaa ggattgtttt tttcagtgtc ggatgtttta tttattttat
      181 ttgagatatt ccgttttgag tttgaaggag attatttatg gaaatcgtga ggttagaaag
      241 tgtttcgcat gcttatcaag ttgataaaac tgcttttaaa agctttttca aaagagagag
      301 cactcaaaaa tacgtcctaa aggatgtatc tttaacgctt aaccaaggag attgtgtcag
      361 tctaattgga ctaaatggtg caggaaaatc gacactcatc aagataatga ctgggttaat
      421 tagaccaacg aatggcactg cttatctttt tggacaggat tctttcttgt gtcgcaaaca
      481 gaatgttcgt gagattggag ttgttttcgg ccaaaaaaca caattatggt gggatttgag
      541 tctgttagaa tcctttgaaa tgctctgccg tatctatggt aataatccta aaaataatca
      601 atggttggat tatttaatag atttgatgga agtaagggat ttttgtagtc gtccaattag
      661 agaactttct ttcggtcaga gaatgagagg cgaatttatt gcttgttttc tacataaacc
      721 aaagattgta tttctagatg agccaacagt aggtcttgat attgtttcta aacgaattat
      781 tgttcatttt ttaagagaaa tcaatcaaac agagggtaca actattcttc ttgcctcgca
      841 tgaacttgat gatgttgaaa aaataactcg ccaaatgtat ctaatagatg ggcagagatt
      901 gaaataccat ggggctattt ctgattttaa ggaggggaga aaacttagaa aagtgataat
      961 acctagtttt cttgcacaag atgaggcaag agagtttgct gtcagtatcc agcaaaatgc
     1021 ttcaatgact gaaatgttga ttgaagaaga gaaattagat ttacttcaag agtttgtcaa
     1081 agaccaaaaa atcagagatt atcatattac agatgcaagt ttagcggata tattattatt
     1141 ggataaggag acatcaaggt gaatgtatta gcttatacaa tagcagaatt taggagacga
     1201 aaatcatata gggtgagtac tattttgaca attcttggtg cctttttggt tgtagccatt
     1261 cagatttcgg tttggaccag tgtagtcact gaaaacacat catctcaact cgttctatat
     1321 gcagtaattt ctaggatgat gttcattctt tttccagatc atcgtaccgc ctccattatt
     1381 tcagagatga ttcaaaaagg tgatgcggca gtctattttt taagacccca tagttttatg
     1441 gggatgcatt tttttagaca agtaggttca tttgtttatg atttgatttt tgtagtcact
     1501 cccttgatgt ttgctcttgt cttttattca ttcgttacag gaatatctct tgttcctgaa
     1561 atggtgctat catttatcat gtcggctcta ttaggtctat tgatttcgtt tttatttggc
     1621 tatctcttag ggcttttatc tatccgtttt aatcagattt taggtgtttt agaattttac
     1681 gatgccttgt tgatgctttt tggtggatca gtattaccta ttagcttttt tccagaaatg
     1741 ttgcaaaaac ttattgtttt aactcctctc tatgctatgt tatcagttcc ttcatctatt
     1801 ttatcggctt tattaccagc ccaatatgtt ttacaacaat tgtgcttaca gatgttttgg
     1861 gttctccttc tggctttatt tatttctatg tatcagaaga agctatttaa agtgatgaac
     1921 tattatggag gttagtgatg acaagatatt tttacttgta tattcatttt atgaaacttc
     1981 aatggaagag gtatatccaa tatagagaag atgccgtcat tggaataata ggctatagcg
     2041 tgaacactgt tatctcaatc tatgttttaa aagtattttt agataatcta tcaaattttc
     2101 ctggatggtc tacaaatgag ttagtactct tgtttggatt tgtaacaatg gccagagctg
     2161 gctggaattt cattttcatc aacaccttat ttattggaga ttatgtcaga gaaggaacat
     2221 ttgatgtctt attacttagg ccagtaaaca ccctattact catcattttt tcaaagatag
     2281 attttgaaag tattggcgag ttttgtattg gtctgataat attttggatg gcactgctta
     2341 cagaatttga aacgttatct gcttggttta tactgaaaat catcctttta ctaataggta
     2401 gtattttatg ttacgcagca atccacctct tcgttaactc attaacattt tttatcaaag
     2461 atgtgtattc cattaacttt attgtttgga gactggatga aacaactcgc tatccaaata
     2521 gtatttatcc tggctggtta tcaaaattat tatggttcat tccatatgca tttattggat
     2581 atttcccact gatgtcaata acaaaatcgt tacctctctt agcagattta gggactattt
     2641 ctgctggcta tatcatcttt ttacttgttt ataagattat ttggaaattt agtattagtc
     2701 gttatcatag ttataggcag taaatttagg gatttatttt ggttaattcg gactattagg
     2761 taaaataaga atagtttatg ttaaaagttt gtaggatcta tataatttgg aactgtaatt
     2821 cagctaattc taagcaaaga attgttaaca atcaaacata acacttgact cagaaaagct
     2881 agtttcaatg tttcgacttt tagctaaaca ttagtctccg ctcgattata ctttgttatt
     2941 gggaggagat acccttttca aaggtcgtcg taaattaagt acttcgaaaa gacactaata
     3001 ttgattcaaa tgtcaaggat atcattaact aagtgaatgt gcttcgtcgt attcagggtc
     3061 aatatccacc attatttttc ctaatttttc tttttgtgat actgttttca aataatcttc
     3121 attattctta taatattctt aagaaaatca agtcttgatt ttcactgggg gaggtggggg
     3181 cggagccacc aagttatcat atcgttagct gtaaaaacta gaaagtttta gtcagctagc
     3241 gatatgattt ttgggatatt gtggacacaa tatctgagct cgcaaaagcc gaaaagcgaa
     3301 acgttgaggc ttttaggaag cacccgtatc tcatacggta tcggggctga cccgatagag
     3361 gcaagtagag aaggagtatc acaaatgaca gaccactatc gcattatccg caaggagatc
     3421 actctgacac caggggaact gaagcagatt caggagctga tgaagcaaga acatgctgat
     3481 cagttttcgc cctttgtccg tcaaaagctg atgaatttag tcgaaaggag gcagtcggtg
     3541 gctgactggt ttgccctctg gcagtcccag aagattgaac aaattagcag ggatattttg
     3601 aaagtcacta ccttggtcga acacactcaa caggtcacag cagaacattt tcacatcata
     3661 ttgacttgtg ttcaggaact gatggtagaa gttggaaaga cgattccact cagttcagac
     3721 ttccgagata agtatatggg aggtcaatga tggaaaaccg ctatcgtacc aatctcaaaa
     3781 aagtatttct aactgatcag gagctagaaa ccttgaataa tcggattgcc aatagcgagt
     3841 gtcagaattt ctccgtctac gccagaaagg ttctacttaa tcccaatatg accttcttga
     3901 cgattgatac cagcaattat caggacttga tttttgagtt gcgacgaatt ggaaacaata
     3961 tcaaccaaat tgctcgagca gttaatcaga ctaaactctt gacagtagag caacgtgacc
     4021 atcttcgatc tggtataggt caactaatta tcgaacttga taaagacttt aatattaagt
     4081 gtcagcaatt gagggagttt tatggtaatc actaagcact ttgcggtgca cagcaataaa
     4141 taccgtaaaa gtctgattag gtatatcctc aatccagaaa agacaaacca actcaaattg
     4201 gtttctgatt ttgggatgag caattacttg gactttccaa cctatgagga gttggttgag
     4261 atgtatcagg ctaattttgt caataatgac aggctttatg attcgagaaa tgatcgacaa
     4321 caaatcaagc aaactaaaat tcatgcccac catctgattc agtccttttc tccggatgat
     4381 aatctgacac ctgaggaaat caaccgtatt ggctatgaga caataaagga gctaacaggt
     4441 ggcaactttc gctttattgt gacgacccac acagataaaa accatctgca taatcatatc
     4501 ctcatcaatt ctattgacct caactctgat aagaaactca aatggaatta tgctcaggag
     4561 agaaacttac gtctgatttc cgactgtatt gccaaagaag cgggagccaa aattatcact
     4621 tataatcgtt actctcatga aaagtttgtg acatatcgga agagtaatcg taaatttgag
     4681 ctcaagcagc gtctttattt tttgatggaa aatagcaagt cctttgatga aattttagaa
     4741 aaagcatctg ccctcaatct tcagattgac ttttctagaa agcatgccag gtttctgatg
     4801 acggacatgg agatgaagca ggtgattcga ggcaatcaac tagacaaacg tcgcccttat
     4861 acagaggaat actttcgaga agaatttgcc aaacgagcga ttaagaaacg tttggatttt
     4921 cttctaccta gagctcagag cttatctcat cttttggaaa tggcagagca gataaatttg
     4981 gtcatttccc tgaagaaaaa gtatgtgact tttcatttga ctgaaaatga tcaaaccatt
     5041 tccctagaaa ataagaaaat cagcaccaaa catttatatg atgtcgcatt ctttgaagac
     5101 cactttgaaa agagagaaaa tactccagaa ctgattcgag agaatcttgt cacggacttt
     5161 gaggattatt gtagtgaagc tgaggaagag aaaatacctt atgaaaaatt gtggaaagcc
     5221 tatgaagagt tcaaggagaa gcgagaccag gtacaggagt ttgaagtggt tttagccggc
     5281 caccaaattg acaagttggt aaaggatgga cttttcatca gagtgaatta tggtgtcaaa
     5341 aaagatggac tagtctttat ctcaaaccgc catttggata ttaaagatac agaggcaggt
     5401 aagtcttatc acgtctttat tcgtgagacc gctcagtttt ttatctatca taaagaagat
     5461 tctatgctta accgctatat gcgagggaga gacttaattc gacagctaac caatgatagt
     5521 aagaccattc ctaagcggag acgactaaaa cttgaaactc tcaaacataa aattgacgag
     5581 attaatcttc ttattgaatt gaacatagac aataaatctt ttcaagaagt taaggatgag
     5641 attatcaaag atattgccca gcttgacctg gctatcacga acttacagga ccatatcgcc
     5701 tatctgaata gagtagcgga agttcttatc aatctaaata atagcgaccc agaaaatcgc
     5761 cgcttagcca gatatgacta tgctaagatg aatttgaccg caggtgcaac tttaagtgca
     5821 gttgaaagtg aacttagaac aaggcagaag gagttgaata atcgtataga taactacgaa
     5881 taccaggtca agaagttgga gaagtttgtt agaatagtaa acgattccat tgatatcaaa
     5941 aaggagaaag tagagaggtt tatagaataa tataaaatgc attcaaaaat atatattatt
     6001 tgaattattt aagaattatt gataaaatag tttgatataa attagtacga ggagaacaaa
     6061 aatggtaatg ttactagctt ctagaataaa gagccgaaga aaagaattaa aattgtctca
     6121 gaaagaatta gctgaaggga tttgtaaaca ggggcaaatt agtaggttag aaaatgggga
     6181 atatactcca gggtcagagt tattatatca gttagctcaa aaattaaatg taagtatgga
     6241 ctattttttt gatgaacaaa tttctgatga aagtactgaa ctgattgact ttaaaaaaat
     6301 agcgaagaac tttgtgtctc aaagaaacta cgagtctttg aaatacatct atgaattgga
     6361 aaaagaaaaa gctcatcgct tatcattatc ggataaaata tatttagaat ggattgcctc
     6421 tttggttgat ttttatgttt atgatagaaa agagacagcg atcgaaaaac tagaaaaggt
     6481 actgacatct ttaaataaaa cagataccaa ctatctccaa atttcaaata cactgtttaa
     6541 cttttatttt gaaacaaata acttatctaa attcgatttt attaaggaag aactgaatct
     6601 tcatctgcaa gtgttgacct taaatacgat agaggagttg gagttatctg ttaaattcag
     6661 ctataatcta agtcgctatt tttggttaca agatgatatt gaatcagcta taaagcaagt
     6721 aacagctact attaaactct gtcaggatta tcgaactact tatctattag cagatttatt
     6781 tttattatta ggcaatctaa gcgagaattt ttcagataaa attatggtga ggagctattt
     6841 tgagatggca tattttttat ataaaaatat agatgaaaat aaagagatgg ctttaacagt
     6901 agagcattat attgcagaaa atttttaaga gcaaattcta ctatagcaca tatggggcag
     6961 ttctaaagag tagacaaact tttagtggct tttttaactt aggtttgtct tttttcttta
     7021 cagttgtaaa gtgtgttctt agacaaatat ttcgtaaaca cgtaataata aggtatagtt
     7081 aactgaaaca agaacatgac aaaagtgctt cgagaaaagt attgcattat ggtgatactt
     7141 ttctgaggtg ttttttttga tgtgaactca tgttttctca atgggattgt actcaggtga
     7201 gtaaggagga agaggtaaaa gtctatgtcc ctgctcctcg cataaatctc ttagcttatt
     7261 catcctatga aaacgtgcat tatccattat gataacagct ggtttgaact aaagttggta
     7321 gcaagaatgt tttgaaccaa gcttcgaaaa agtcactcgt catagtatct tggtatgtca
     7381 tcggagctat aagctcacca tttatgagac ctgcaaccaa agatcgccgc agataccttc
     7441 ttccagagac ctgacctttt attaactgac ctttcaaaga gcgagcatat tctcgatgaa
     7501 aatatgtctc aaaccctgtc tcatcaatat aaacaggagt cagatggctt acggcagtaa
     7561 cttgaggctg ggattgaaaa agcgaaggta gttattctct caaagctttt ggggagacaa
     7621 gtcgacagct gtcattttga gtcttttaga aacagctaag cgacactgac ttgatgcaca
     7681 gaaatatttg ggctatcttc tagaacactc acctaatgag gagaggctcg caaaaaagaa
     7741 gatgctagag gcttagagcc atgggaggaa aaaattaaag agagtatgta aatagaaaat
     7801 ggttccagag tcgacaagac tttgaagcct ttttaataca ctttattatt gggcggttac
     7861 aatatttcta gtccaaggat tacaatcacg agaagagtct tcgtcttatt caatttaatc
     7921 tataattttg ttataaaatg ttatacagtt atgatgaaaa ttaaagcgga aaaattattt
     7981 tttatatatt agataatttt ttcaagaata caaaataggg ttaaaaaaaa aaagtataaa
     8041 aaaagtttat ataaaatatt gacaaagtgt ttattaagtg ttagaataag cttacaaaat
     8101 ataaaggaga tttatatgaa gcaatttttg aaaaagatta ttgttgcatc tttattggga
     8161 ggtttacttt tagcgattgg gaacagtgga ataaataagc ctagaccaat gggaggaatt
     8221 gacccaggcc cagctaatac ttacgtgaaa tagaattggt ttgagagagt tttgtgaatc
     8281 aattttgtaa acatgtttaa attttaacga aggaggtatt tgatatgagc aaacttgaag
     8341 taaataaaga gttagcggta aatgaattga actttatcaa aaaagtttca atttcaaaac
     8401 cagttgtggc ccagtcgaaa tgtagatatt ctactgcgac aacgagaatc taagaattac
     8461 tcggtttagc aacctacttt taggtagtta tttgttgatt ttagagataa gggctggaat
     8521 ttccagcctt ttttggtatt aataaaaatg agagaactag aagtaatatt tataaataat
     8581 aaaaataaat atttttgtaa tataggagaa caattctttt tgttatcaga gaaatatatc
     8641 aaagaag
//
LOCUS       DATUCG010000104         8640 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.106, whole genome shotgun sequence.
ACCESSION   DATUCG010000104 DATUCG010000000
VERSION     DATUCG010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8640
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.106"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(81..845)
                     /locus_tag="VCA20_001847"
     CDS             complement(81..845)
                     /locus_tag="VCA20_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEP1835618.1"
                     /translation="MIKDERVLELIEVIKKKKRIAVKELVEITFSSLSTLRRDLIFLE
                     NQGLIKRKHGYVTLSSINTMELSHQIREGESTRQKRFIANLAKDFIRSGMCIYLDSST
                     TVYELCPHLAELDNLIIFTNGLHTAQTLSETVRDNSKIFITSGEVKHQSCSVVNYDKE
                     NSLLDHFNIDLAFCSARGIDDQYVYEASLSQAISKKSIIDKAHETILLIDSSKFYKTG
                     FFKINPLSKYTTFISDSMPDQKLLDAVELFDGEWAY"
     gene            1022..2425
                     /locus_tag="VCA20_001848"
     CDS             1022..2425
                     /locus_tag="VCA20_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HEP1835619.1"
                     /translation="MEAVLAIDLGATSGRAIVGYLSENKLVMEEINRFSNLPIRVKGH
                     LSWDVDFLLAKIFESIRLANTSYKILSIGIDTWGVDFGLIDKEGQLLSQPVHYRDERT
                     KGVLKEISEITELEKLYSETGNQLMEINTLFQLFKVRQEFPNSFYSTNKILLMPDLFN
                     YLLTGKFATEKSIASTTQLFDPRSQKWNQNILKLFELDSSLLPEIVSEGNVLGRIKEE
                     YGLGGIPVVNVCSHDTASAIVSVPKTGDSLFISSGTWSLVGVELSSPILCTESFSYGF
                     TNEVGKDGVITFLKNCTGLWIIEELRRSFERRGKAYSFGDIKTMVEKDKGNLPLIDTE
                     STEFATESDMYKTLTEYLEHRYGTREWTDGQLFKIVYESLAETYKKTIELLEELTHKV
                     YKRIYVIGGGARASYFNQMIADRTGKEVLTGSTEATAVGNIVVQLIAIGQLKGMEEAH
                     HVIEEFLQLESYYSQKN"
     gene            2540..3178
                     /locus_tag="VCA20_001849"
     CDS             2540..3178
                     /locus_tag="VCA20_001849"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HEP1835620.1"
                     /translation="MSDVKQELIKYGKKLVETDLTKGTGGNLSVFDREKQLMAITPSG
                     IDFFEIKESDIVVMDINGNVVEGERLPSSEWYMHLIQYQTRDDIDAIIHAHTTYATVL
                     ACLREPLPASHYMIAVAGKDVRVAEYATYGTKELAVNAAKEMEGRRAVLLANHGILAG
                     AQNLLNAFNIVEEVEYCAKIYCLAKNFGEPVVLPDEEMELMAEKFKTYGQRK"
     gene            3190..3630
                     /locus_tag="VCA20_001850"
     CDS             3190..3630
                     /locus_tag="VCA20_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU family protein"
                     /protein_id="HEP1835621.1"
                     /translation="MLKHIPKNISPDLLKTLMEMGHGDEIVLADANYPSASCANKLIR
                     CDGVNIPDLLDSILYLIPLDGYVNSSIQFMNVVSGDDIPKIWGTYRQMIEGHGTDLKT
                     ITYLRREDFYERSKKAYAIVATGETSLYANIILKKGVVVERENV"
     gene            3623..4054
                     /locus_tag="VCA20_001851"
     CDS             3623..4054
                     /locus_tag="VCA20_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEP1835622.1"
                     /translation="MFNRGILVASHGNFASGSLMTAEMFVGETTNDRVRTLGLMPGEN
                     IVEFEHYFKNQVDELLDSNQEVIVLTDLIGGSPNNVALSQFLNLDSVDIVTGFNIPLL
                     VELISSYDSKINLEEIVHNAQNSLFNVKQQLNVEEEEDLCL"
     gene            4045..4515
                     /locus_tag="VCA20_001852"
     CDS             4045..4515
                     /locus_tag="VCA20_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEP1835623.1"
                     /translation="MSIEFVRIDDRLVHGQVVTTWLKKYDIEQVIIVNDRISEDKTRQ
                     SILKISAPVGLKIVFFSVKRFVEVLNSVPIKKRTMLIYTNPKDVYDSIVGNLKLEYLN
                     VGQMSKTEKNEKVTGGVALGEEDKYYFKKIVDKGTRVEIQMVPNDKVTMLEKFL"
     gene            4538..5311
                     /locus_tag="VCA20_001853"
     CDS             4538..5311
                     /locus_tag="VCA20_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEP1835624.1"
                     /translation="MPFTQALLVTLVGIIATIDYNGPLFMIHRPLVTSAMVGLVLGDF
                     TQGVLIGSALELTWLGVTGIGGYTPPDTISGAIIGTAFGILSGQGETAGIAIAVPIAV
                     ATQQLDVLAKTLDVYFVKKADNDAKNGDYSKIGFYHYSSLVLITLFKIVPIFLAIMLG
                     GEYVADLFAKVPPIVMQGLNSAGALLPSIGFGMLLNMMLKKNMWVFLLIGFICSVYGG
                     MSTIGISLVGIAVAYFYDMIGSKPQETTSNSDVEEDLDL"
     gene            5308..6108
                     /locus_tag="VCA20_001854"
     CDS             5308..6108
                     /locus_tag="VCA20_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEP1835625.1"
                     /translation="MMNNKVTKVELKKVFKRSFMYGSSWNYERMQNLGFLYTILPVLK
                     KLYPDKDLASPAMKRHLEFFNTHQTAAPFILGVTSAMEEQEGNEGADSITGIKVGLMG
                     PLAGLGDSLFWLTLVPICFSIGASYSKDGGALGIFIALMLFNVINIPVKYFGLKYGYT
                     KGSSLIQENNTKGTLNRITSMATALGLVLVGGLIPSMVGINFGLEFKQGELVISVQEM
                     ITTLIPGFIPMALTLLMCKLIRKGKNPVVLIFSVMAIGVILVVLGILK"
     gene            6166..8457
                     /locus_tag="VCA20_001855"
     CDS             6166..8457
                     /locus_tag="VCA20_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEP1835626.1"
                     /translation="MKLWYKKAASNWNEALPIGNGHLGGMIYGSAVKECIQLNDETIW
                     YRGKSDRNNPDSLLHLKKVREYLLDGEIQKAEELIKLTMFATPRDQSHYELLGELYIE
                     HIDIQPSALSLYERELDLDTAISNVIFEPNSCNLQIKREYFTSFNKNILCCRIVSSVQ
                     NTLNLNINLGRNKRFNDEVSKLDSSTILMSASAGGRKGVRFKVVCHSKVTDGEVNVLG
                     ETIVIRNATEVFLYLKSMTDYWGNLDISSLQGEFSSIDYFTEKDEHVKKYQEQFNRVD
                     FKLDYSKDCLSIPTNLLLEDTKKYSNYLTNLLFHYGRYLLISSSQPNGLPANLQGIWC
                     DELNPIWGSKYTININTQMNYWMVGPCDLPEVEYPLFDMLERMREPGRLTAKKMYGAR
                     GFTAHHNTDGFGDTAPQSHAMGAAIWVLTIPWLCTHIWEHYLYFQDERILREHFEMIK
                     EAFLFFEDYLFEVDGYLMTGPSVSPENKYRLKNGIEGNACLSSTIDNQILRYFCDSCI
                     GIAKQLVDNSDFISRVKELKKKLPKTKIGSNGQIQEWLEDYEEVEPGHRHISPLFGLY
                     PYNEIDIHKTPELAEAAKITINRRLSNANFLSSQDREQAINNWLVSGLHASTQTGWSA
                     VWLIHFFARLYQGEPAYNQINGLLNNATLGNLFLDHPPFQIDGNLGLVSGICELLVQS
                     HHNWLSLIPALPSAWSAGEVKGLRVRGGYKVSFAWKNRDITFLRLEGGDSGKAVKIKI
                     GNKTNERCIETIFNSEQVIELNL"
BASE COUNT         2876 a         1156 c         1787 g         2821 t
ORIGIN      
        1 tttggtaata gattccagtt atttaagatt agtaaatgaa gtttttatga atgaatatag
       61 tagaaattga gtaatttgac ttaatatgcc cattctccat cgaataattc tactgcatct
      121 aataattttt ggtctggcat gctatcagaa ataaaggttg tatatttaga aagtggatta
      181 attttgaaaa atccagtttt gtagaatttt gaactatcaa tcaacaaaat ggtttcatgt
      241 gctttatcaa taatactctt ctttgaaatt gcttgactta aagatgcttc ataaacatat
      301 tggtcatcaa tgcctcttgc tgaacaaaaa gccaaatcta tattaaagtg atctaataaa
      361 gaattttctt tatcatagtt gactacagaa caagattgat gctttacttc tccagacgta
      421 ataaaaattt tagaattatc tctaacagtc tcagataagg tttgtgctgt atgtagacca
      481 tttgtaaaaa taattagatt atctagttcg gcgagatgag gacagagttc gtagacagtc
      541 gtactggaat caagataaat acacatacca gagcgaataa aatctttggc cagattagct
      601 atgaatcttt tttgacgagt actttctcct tcacgtattt gatgagaaag ttccattgtg
      661 ttaatagagg aaagagttac ataaccatgt tttcgcttga taaggccttg attttctaaa
      721 aaaattaaat cacgacgtag agtactaagg ctggagaaag tgatttctac aagttctttt
      781 acagcaattc tttttttctt tttaataact tcaatcagtt caagtacacg ttcatctttt
      841 atcatagtat cctccaatat aaaacttcaa ctatattata gcataatttg gaggaatttg
      901 aattattttt gtcaatttca ggtttaatat atgaatttta ttcaagtaag cgttcacata
      961 ttgaaaaata atcctatgat attataattg tgttaacaag ggtattggag agggttaata
     1021 aatggaagcg gttttagcaa tagatttagg tgcaacttct ggaagagcaa tcgttggtta
     1081 cctttctgaa aataaactag taatggaaga aataaatcgc ttttctaatc tacctattag
     1141 agtaaaaggg catttatctt gggatgttga ctttctacta gctaaaattt ttgaaagcat
     1201 ccgcttggct aatactagtt acaagatttt atctatcggt attgatacat ggggtgttga
     1261 ttttggactt attgataagg aaggtcagct gttatcacaa cctgttcatt atcgtgatga
     1321 aagaacaaag ggagtgttaa aggaaatatc tgaaattact gaattagaaa aactgtattc
     1381 agagacagga aatcagctta tggagattaa caccttgttc caactcttta aggtacgaca
     1441 agaatttcct aattcgtttt atagtacgaa taagattctt ttaatgccag atttgtttaa
     1501 ttatctgttg acaggtaagt ttgctacaga aaaaagtatt gcttcaacaa ctcaactatt
     1561 tgatcctagg agtcaaaaat ggaatcagaa tattttaaaa ctatttgaat tagattcatc
     1621 tttacttcct gaaattgttt cagaaggaaa tgtccttggt aggattaaag aggaatatgg
     1681 tttaggcggt attcctgttg tgaatgtttg tagtcatgat acagctagtg cgattgtatc
     1741 agttcctaag acgggagata gtttgtttat ttcatcagga acttggtctt tggttggagt
     1801 ggagctttct tcacctattc tttgtaccga atcgtttagt tatggattta caaatgaagt
     1861 cgggaaagat ggagtgatta catttctgaa gaattgtaca gggttgtgga tcatagagga
     1921 actaagacgt tcatttgaac gaagagggaa agcctattct tttggtgata taaaaacaat
     1981 ggtggagaaa gacaaaggaa atcttcctct gattgatact gaatcaactg aatttgcaac
     2041 agaatctgac atgtacaaga ctttgacaga atatctagag catcgttatg gtactagaga
     2101 gtggacagat ggacaactat ttaagatagt ttatgaaagt ctagctgaaa cgtataagaa
     2161 aacgatagag ttactagaag aactaactca taaggtttat aagagaatat atgtgattgg
     2221 aggaggtgct agagcaagtt actttaatca aatgattgct gatagaactg gtaaagaggt
     2281 tcttacaggt tcaactgagg ctacagcggt agggaatatt gttgtgcagc tcatagcaat
     2341 aggacaatta aaagggatgg aagaagctca ccatgttatt gaagagtttc tacaattaga
     2401 gagttattac tcccaaaaga attagaaaga ttgagagttt gtaaatttgc ctccctccct
     2461 cttcttagct tttgtgcaag aagaggggat aattggtgaa ttgaaaaaac tttagtgttt
     2521 tgatatgagg aggacaagga tgtcagatgt aaaacaagaa ttaattaaat atggtaagaa
     2581 gttagtagaa acagatttga cgaaaggaac aggtggaaat ctcagcgttt tcgatcgtga
     2641 aaaacaattg atggcaatta ccccgtcggg tattgatttc tttgaaatca aagaatccga
     2701 tattgtagtg atggacatta atggaaatgt tgtagaggga gaacgcttac catctagcga
     2761 gtggtatatg cacttgattc aatatcaaac tcgtgatgat atcgatgcaa ttatccatgc
     2821 tcatacaact tatgcaacag tattagcttg tctcagagaa ccacttccag cgagtcatta
     2881 tatgattgca gtggcaggga aagatgttcg ggtagctgag tatgcaacat atggtacgaa
     2941 agaattagct gttaatgcag ctaaagaaat ggaaggtcgt agagcagttt tactagcgaa
     3001 tcatggaatt ttagcaggtg cacaaaattt attgaatgca tttaatattg ttgaagaagt
     3061 tgaatattgt gcaaaaattt attgtttagc taagaatttt ggagagccag tagttcttcc
     3121 tgatgaggag atggagttaa tggcagaaaa atttaaaaca tacggtcaga gaaaataggg
     3181 aggatattaa tgttaaaaca tataccgaaa aatatttctc cagatttatt gaagacttta
     3241 atggaaatgg gacatggaga tgaaatagta ttagctgacg cgaattatcc ttctgcctca
     3301 tgtgcaaata agctaattcg ttgtgatggt gtaaatattc ctgatttatt agattctatt
     3361 ctgtatttaa taccattaga tggttacgtc aatagttcaa ttcaatttat gaacgttgtt
     3421 tcgggggatg atattcctaa gatatggggt acctatagac agatgattga aggtcatggt
     3481 acagatctta aaacgattac ttatcttaga agagaagatt tttatgagcg tagtaagaaa
     3541 gcttatgcta ttgttgctac aggagaaact tcactttatg ctaatattat ccttaagaaa
     3601 ggagtagttg ttgaaagaga aaatgtttaa tagaggaatt ttagttgcta gtcatggtaa
     3661 ttttgctagc ggatctctca tgaccgcaga aatgtttgtt ggtgagacaa caaatgatag
     3721 agttaggaca ttaggtttga tgcctggaga gaatattgta gagtttgagc attattttaa
     3781 aaatcaagtg gatgaactgt tagactcaaa tcaagaggtt atcgttttga ctgacttgat
     3841 tggaggaagc cctaataatg tggctttgtc acagttttta aatttggatt cagttgatat
     3901 tgtaacaggt tttaatattc ctctcctagt ggaattaata tcaagttatg attcaaaaat
     3961 caatttagaa gaaattgttc acaatgctca aaatagtttg tttaatgtta aacaacaact
     4021 taacgtagag gaggaagagg atttatgtct atagagtttg ttcgtattga tgaccgtctg
     4081 gtacatggtc aagttgtaac tacgtggcta aaaaagtatg atattgagca agttatcatt
     4141 gttaatgatc gcatctcaga agataaaaca cgacaatcta ttttaaagat ttctgcaccg
     4201 gtaggtttaa aaattgtttt ctttagtgta aaacggtttg tggaagtttt aaactctgtg
     4261 ccaataaaaa agagaacaat gttgatatat accaatccaa aagatgtgta tgactctatt
     4321 gtgggaaatt taaaattgga gtacctcaat gtaggacaga tgagtaaaac ggagaaaaat
     4381 gaaaaggtaa ctggaggtgt agctctaggt gaagaagaca aatattattt taagaaaata
     4441 gttgataagg gaacgagagt tgaaattcaa atggttccca atgataaagt tacaatgttg
     4501 gaaaaatttt tataaataat ttaaggaggt acagtatatg ccattcacac aagcattact
     4561 ggtgacatta gttgggatta ttgccactat tgactataat ggaccgttat ttatgattca
     4621 ccgtccgtta gttacaagtg caatggttgg cttagtctta ggagatttca cccaaggtgt
     4681 tcttattggt tcagctcttg aattaacttg gcttggtgta acaggtattg gaggctacac
     4741 tccaccagat actatttcag gtgcgattat tggtactgca tttggtattt tatctggaca
     4801 aggagaaact gctggtatcg ctatagcggt tccaattgca gttgctaccc aacagttgga
     4861 tgttcttgca aaaactttag atgtttattt tgtgaaaaaa gctgataatg atgctaaaaa
     4921 cggagattac tcaaagattg gtttttatca ttattcaagt ttggttttaa tcacgttgtt
     4981 taaaattgtg ccaattttcc tagctattat gcttggaggg gaatatgtgg cagacttgtt
     5041 tgctaaggtt ccgccaatcg ttatgcaggg acttaactct gcaggtgctt tactaccttc
     5101 aattggtttt ggtatgcttt taaatatgat gctcaagaaa aatatgtggg tattcttgtt
     5161 gattggattc atttgttctg tgtatggagg aatgtcaacc attgggatct cactagttgg
     5221 tattgcggta gcatacttct acgatatgat tggaagtaaa ccacaagaaa caacttcaaa
     5281 tagtgatgtt gaggaggatc ttgatctatg atgaataata aagtaactaa agttgaactt
     5341 aaaaaagttt tcaaacgaag ttttatgtat ggttcttcat ggaactatga gagaatgcag
     5401 aacctaggtt ttctatatac aattcttcca gtattgaaaa aactatatcc agacaaagat
     5461 ttggcttctc ctgcaatgaa acgtcacctt gagtttttca atactcatca aacagcggca
     5521 ccatttattc ttggagttac ttccgctatg gaagaacaag aaggaaatga aggtgcagat
     5581 tcaattactg gtattaaagt tggcttgatg gggccactag ctggtctagg agatagtttg
     5641 ttctggttga cactagttcc tatctgtttt agtattggtg cgtcttattc taaagacggc
     5701 ggtgctttag gtatctttat cgccttaatg ttgtttaatg ttattaatat tcctgttaaa
     5761 tatttcggtt tgaaatatgg ctatactaag ggttctagtc ttatccaaga aaataataca
     5821 aaagggacat tgaatcgcat tacgagtatg gcaacagcat taggtctagt actagtgggt
     5881 ggtttgattc catcaatggt tggtattaat tttggattag aatttaagca gggagaactt
     5941 gttatttctg ttcaagaaat gattacaaca ttaatcccag gatttatccc tatggcttta
     6001 actttattaa tgtgtaaatt aattagaaaa ggaaagaatc cggttgtact aatctttagt
     6061 gttatggcta ttggagttat cctagttgtt ttaggaattt tgaagtagta gaaagtgtgg
     6121 aggtggtatt tgggatatca ccccattttg gaagagaggt aaagagtgaa attatggtat
     6181 aagaaagctg cctcaaattg gaatgaagcc ttgccgattg ggaacggtca tttaggtggt
     6241 atgatttatg gttcagctgt aaaagaatgt attcaactaa atgatgagac tatttggtat
     6301 agaggaaagt cagatagaaa taatccagac tcactattgc atcttaaaaa agttcgggaa
     6361 taccttttag atggagaaat tcagaaagcc gaagaattga taaaattaac aatgtttgct
     6421 actccaagag atcaaagcca ctatgaatta ctgggagaac tttacattga gcatatagat
     6481 attcagcctt ctgctctttc attgtatgaa agagagctag atttagatac agctatttcg
     6541 aacgttatat ttgaaccaaa tagttgtaat ttacaaataa aaagagaata ttttacgagt
     6601 tttaataaga atattttatg ttgccgtata gtgtcatcag tccaaaacac attaaattta
     6661 aacattaatc tgggtagaaa taaacggttt aatgatgaag tatctaaact ggattcaagt
     6721 acaattttaa tgtcggcctc tgctggaggt agaaaaggtg ttcggtttaa agtagtatgt
     6781 cattctaaag ttacggatgg tgaagtaaat gtattgggag agacaatagt tattcggaat
     6841 gctacagagg tatttcttta cctcaaatca atgacggatt attggggaaa tctagatatt
     6901 tcttctcttc agggagaatt tagtagtatt gattacttta cagaaaaaga tgaacatgta
     6961 aaaaaatatc aggaacaatt taatagagtt gattttaaac tagactatag taaagattgt
     7021 cttagcattc caacgaatct acttcttgaa gacactaaaa agtatagtaa ctacttgact
     7081 aacttgttat ttcattatgg aagatatctg ttaatatcgt ctagtcaacc aaatggttta
     7141 cctgccaatc ttcaaggaat atggtgtgat gaattaaatc caatttgggg ttctaaatat
     7201 acgattaata tcaatactca aatgaattat tggatggtag gtccatgtga tttaccagaa
     7261 gtagaatatc cattatttga tatgctcgaa agaatgagag aaccgggaag actaaccgct
     7321 aagaaaatgt atggagctag aggttttaca gcacatcata atacggatgg ttttggcgat
     7381 acggctcccc aatctcatgc catgggggct gcaatttggg tattaactat tccatggtta
     7441 tgtactcata tttgggaaca ctatttatat ttccaagatg agcgtatcct tagggaacat
     7501 tttgaaatga taaaagaagc atttcttttc tttgaagatt atttatttga ggtggatggc
     7561 tacttgatga caggtccaag tgtctcaccg gaaaataaat atcgcttaaa aaatggtatt
     7621 gaaggaaatg cttgtctatc atctacaatt gataatcaaa ttctaagata tttttgtgat
     7681 tcatgtattg gtattgcaaa acaattagta gacaattcgg attttattag tcgtgtgaag
     7741 gagttaaaaa agaaattacc taaaacaaaa ataggtagta atgggcaaat acaagaatgg
     7801 ctagaggatt atgaagaagt agagccaggt cacagacaca tttcacctct atttgggctt
     7861 tatccttata atgagattga tattcataaa actcctgaat tagcagaagc agctaaaatc
     7921 actataaata ggagattatc aaatgctaac tttttatctt cacaggatag ggaacaagcg
     7981 attaataatt ggttagtaag tggtttgcat gctagtacac aaacaggttg gagcgctgta
     8041 tggttgattc atttttttgc gagactatat caaggtgaac ctgcttataa ccagattaat
     8101 ggtttgttaa ataatgcgac tcttgggaat ttatttcttg accatccacc atttcaaatt
     8161 gatggtaatt taggtttggt gagcggaatt tgtgaattat tagtacagag ccatcataat
     8221 tggttatcac ttattccagc tttaccttct gcttggtcag caggagaagt gaaagggctc
     8281 agagtaagag ggggatataa ggtatcgttt gcttggaaaa atagggatat aacttttctg
     8341 agattagagg ggggagattc aggtaaagca gtaaaaataa aaattggtaa taaaacgaat
     8401 gagcgatgta ttgagacaat atttaattca gaacaagtta tcgaattaaa tttgtaggta
     8461 taagcaaata atactcgtgg tgctagttca tttcaaacta caataaaaag cccaaatgga
     8521 ctatactgta agtgacgaaa catacaggag gttagtccaa atgagctact cacagtatac
     8581 cgctaaatct catcatttgc aatggagtgt acgccaatta tcacaaattt gtcatcactt
//
LOCUS       DATUCG010000105         8633 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.107, whole genome shotgun sequence.
ACCESSION   DATUCG010000105 DATUCG010000000
VERSION     DATUCG010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8633
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.107"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(32..1702)
                     /locus_tag="VCA20_001856"
     CDS             complement(32..1702)
                     /locus_tag="VCA20_001856"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEP1835627.1"
                     /translation="MKTDIDIAQSITLKPITEIVEKVGISFDDIELYGKYKAKLSFDK
                     INAVKGNPLGKLILVTAINPTPAGEGKSTITIGLADALSKIGKKTMIALREPSLGPIM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHIHQGNVIGIDQRR
                     IIWKRVVDLNDRALRKVTVGLGGPLNGIPREDGFDITVASEIMAILCLATDINDLKER
                     LANIVIGYRFDRSPVYVRDLAVEGALTLILKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSVLATTTALRLSDYTVTEAGFGADLGAEKFLDIKVPNLPKAPDAVVIVATLRAL
                     KMHGDVAKTELSAENVEAVKAGFSNLKRHVENIQKYGIPAVVAINEFVSDTADEIAAL
                     KELCAEIGVPVELASVWANGADGGVELAETVVATIDNQAANYKRLYKAEDSLEEKVTK
                     IVTQIYGGTGVVFEKKARNQLAEFAKNGWDKLPVCMAKTQYSFSDDQFALGAPTDFDI
                     TVREFVPKLGAGFIVALTGDVMTMPGLPKAPAALNMDVAADGTAIGLF"
     gene            2103..2798
                     /locus_tag="VCA20_001857"
     CDS             2103..2798
                     /locus_tag="VCA20_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEP1835628.1"
                     /translation="MKLLITSGGTSEAIDQVRAITNHASGNLGKIIAEQALKRGHEVT
                     LVTTRQAVKPDSQKNLTIIEITNVDSLKETLEPLVKSHQALIHSMAVSDYTPVYMAGL
                     DEIKATEDISSLLKKQNTESKISSKDDYQVLFLKKTPKVISYVKKWNPAITLFGFKLL
                     VNVPKEELFAVARQSIERNGADYILANDLKDIGENQHIAYLVDKTREIQAQTKDEIAQ
                     LILNTLEKGEQNG"
     gene            2791..3339
                     /gene="coaC"
                     /locus_tag="VCA20_001858"
     CDS             2791..3339
                     /gene="coaC"
                     /locus_tag="VCA20_001858"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEP1835629.1"
                     /translation="MANITLAVTGSISAYKAADLTSQLTKLGHQVTVLMSRSAMDFIT
                     PLTLQSLSKNLVHTDVMIEENPTLIKHIDIAKETDVFLVAPASANTIAKLANGMADNI
                     ITATALALPIGTKKLLAPAMNTNMYLNPATQKNIKTLMEYSFEEIKPREALLACGDFG
                     TGALAEIDVILNKVMEVLNENK"
     gene            3326..3895
                     /locus_tag="VCA20_001859"
     CDS             3326..3895
                     /locus_tag="VCA20_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEP1835630.1"
                     /translation="MKTNKSRDVATLAIFLTIMIVIEVVSQMIFANFILPIKPTITHI
                     PIIIASILYGPRLGAQLGGFMGIMSIIRNSIIISPMSYVFSPFVEHGNFNSILIALVP
                     RILIGVTPYFVYKLMKNKKGLLLAGLTGTLTNTIFVLTGIFFLFSNVYAGDIKLLLAS
                     IVSFNSIAELTISGLLTIAIVPVLQKVQK"
     gene            3986..5704
                     /locus_tag="VCA20_001860"
     CDS             3986..5704
                     /locus_tag="VCA20_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEP1835631.1"
                     /translation="MTYQETYQTWLDFADLPDYLREELVAMDEKTKEDAFYTNLEFGT
                     AGMRGYIGAGTNRINVFVVRQATEGLAKLVESKGEEAKKRGVAIAYDSRHFSPEFAFE
                     SAQVLAAHGIKSYVFESLRPTPELSFAVRHYNTIAGIMVTASHNPKEFNGYKVYGEDG
                     GQMPPADADALTNFIRAIDNPFVVELADLEASKENGLITVLGEETDVKYLEELKDLNI
                     NPELIAEYGKDMKIVYTPLHGTGEMLARRALAQAGFESVQVVEAQATADPDFSTVASP
                     NPESQAAFALAEELGRQVGADVLLATDPDADRVGVEVRQADGSYWNLSGNQIGAIIAK
                     YILEANKQAGTLPANAALAKSIVSTELVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMFGFEESFGYLIKPFVRDKDAIQAVLMVAEIAAYYRSRGMTLADGIDEIFKE
                     YGYFAEKTISVTLSGKDGAEQIKAIMAKFRDNSPAQFNATDIAVFEDFALQTKTDKDG
                     NVEKLTTPPSDVLKYTLADDSWFAVRPSGTEPKIKFYIATVGETLAEAEEKIANIEAE
                     INAFVG"
     gene            complement(5955..6401)
                     /locus_tag="VCA20_001861"
     CDS             complement(5955..6401)
                     /locus_tag="VCA20_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013085824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEP1835632.1"
                     /translation="METTVYIRQYLQEDFERLCQIHDPARQEKLDLANLSEAFIPLSI
                     ATDREGLFDYQILVAEMEGFVVGFVAFTDDELAWLYVDREYSRHGIGTALVNVVLKKM
                     HEPISIEVLTGNTPALNFYRKFGFEIVKTESGQMQGMKSFLLPFIH"
     gene            6427..6615
                     /locus_tag="VCA20_001862"
     CDS             6427..6615
                     /locus_tag="VCA20_001862"
                     /inference="COORDINATES: protein motif:HMM:NF023227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835633.1"
                     /translation="MKKSVNAQKKIDPANLPKIMVSHVLELFQKKYSSGAVRQIGVSY
                     SGFVDENYTILYYHSLIM"
     gene            6782..7149
                     /locus_tag="VCA20_001863"
                     /pseudo
     CDS             6782..7149
                     /locus_tag="VCA20_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7136..7435
                     /locus_tag="VCA20_001864"
     CDS             7136..7435
                     /locus_tag="VCA20_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835634.1"
                     /translation="MNQLEFQRNHLQMDYYSDSYQDFERDFYRYSNMNIPLTFLTDDI
                     LKTMATSCKNYFVLNKEKSKDNRDHFFIFEVSTVDENPLIYRYSYKKTTTNLTEK"
     gene            complement(7591..8406)
                     /locus_tag="VCA20_001865"
     CDS             complement(7591..8406)
                     /locus_tag="VCA20_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEP1835635.1"
                     /translation="MKRKQWLVHLILSFGALSMILPFLWMLLTSGKTLQEATAIPPTI
                     FPKTVQVSNYTEVLNLLPFMAFYFNTFLMVVGRVAGSVLFSAMAAYATARLEFPGKRI
                     FFGIVVVQMMIPGSIFITPQYLLVQKIGLLNTVPALVVPGIVSAFGTFLLRQFFLGLP
                     KELEEAAYLDGANIWTIFFKIMLPLARSGLIALSIFTALFAFKDLMWPLIVNTSIDKM
                     NLAAGLANLQGQYSTNYPQLMAGSLLAIWPMIVLFIIFQKQFIEGIATSGGKL"
BASE COUNT         2721 a         1830 c         1585 g         2497 t
ORIGIN      
        1 tccgcctttg agcggatttt ttatattctt gttagaacaa gccgatagct gtcccatctg
       61 ccgccacatc catgttgaga gctgcgggtg cttttggcaa accaggcatg gtcatgacat
      121 cacctgttaa ggcaacgata aagcctgctc ctaattttgg aacaaattca cggactgtaa
      181 tgtcaaagtc tgtcggcgct ccaagggcaa actgatcatc tgagaagctg tattgggtct
      241 tggccatgca gactggcaac ttatcccagc cattcttagc aaattcagcc agctggttac
      301 gagccttctt ctcaaagaca acgcctgtac caccgtagat ttgcgtaaca atcttggtca
      361 ctttctcttc taagctatcc tcagccttgt aaagacgttt gtagtttgct gcttggttat
      421 caatagtagc aacaactgtc tcagccaatt caacaccacc atcagcgcca tttgcccaaa
      481 cacttgccaa ctcgacaggt acgccaattt cagcgcaaag ttccttcaaa gcggcaattt
      541 cgtcagctgt gtcagataca aattcattga tagcaacgac tgctggaata ccgtatttct
      601 gaatattttc aacgtgacgc ttcaagttag aaaaaccagc tttcactgcc tctacatttt
      661 cagcagaaag ctctgtcttg gctacatcac cgtgcatttt aagcgcacgg agggtggcaa
      721 caatgactac tgcatccgga gccttaggca agtttggcac cttaatgtca aggaacttct
      781 ctgctccaag gtcagccccg aaacctgctt ctgtgacggt atagtcggac aatcgcagag
      841 cggttgtggt agcgaggact gagttacagc catgagcaat attagcaaat ggacctccgt
      901 ggacaaaggc tggcgtgcca tagatagttt ggacaaggtt tggtttaata gcgtccttca
      961 aaataagagt caaagcacct tccactgcca aatcacgcac gtagacagga ctgcgatcga
     1021 aacgataacc gataacaata tttgccaagc gttctttcaa gtcattgatg tctgtcgcca
     1081 agcacaaaat cgccatgatt tcagaagcga ccgtgatatc aaaaccatct tcacgtggaa
     1141 taccattgag cgggcctccc aagcccactg ttaccttacg aagggcacga tcgttgaggt
     1201 ccacaacacg tttccagata atacgacgtt gatcaatccc aatcacattc ccctgatgga
     1261 tatggttatc aatcaaggct gaaagggcgt tgtttgctgt ggtaatggca tgcatgtcgc
     1321 ctgtaaaatg caaattgatg tcttccatag gcaagacctg agcatagcca ccacctgcag
     1381 ctccaccttt gatccccatg ataggtccca aagaaggctc acggagggca atcatagttt
     1441 tcttgccaat tttagacaag gcatctgcca aaccgatagt aatagtcgac ttgccttcac
     1501 cagctggcgt tgggttgata gctgttacta aaatcaactt acccagtgga tttcccttga
     1561 ccgcattgat tttatcaaaa gacaattttg ccttgtattt tccatatagt tcaatatcat
     1621 caaaactgat accaactttt tcaacgatct ctgtgatagg tttcaaggtt atactttgtg
     1681 caatgtcaat atctgttttc atgagagtac ctaatttcct tattttttat ggatattata
     1741 ccatactaca acttattttc gcatattttt tactatttat tcactaacgt acggaatttt
     1801 cagaaaaact acaggtttct ttacttaaac agatacaaat ttaaaaaaac tgcattctca
     1861 tcatagctct aaacttccat ctaagactgg ttttagttag tttttttaaa agagagtcag
     1921 tattccttta aaaattgcct tgatgaacaa accaacgctt tcttgcacaa aaacacttca
     1981 ctatataata caggccctta tattttttgt gttagcagca tcattttcca gtgtcacaga
     2041 ttgattaaca aatagattag tgcaaacttt acgaaaaata gaaattctag taaaatatga
     2101 acatgaaact attgattaca tcaggcggaa caagcgaagc tatcgatcaa gtccgcgcta
     2161 ttacaaacca tgcttctgga aatcttggaa aaatcattgc cgaacaagcc ttgaaacgcg
     2221 gtcatgaagt tactcttgtg actacaagac aggctgttaa accagattct cagaagaatt
     2281 taaccattat cgaaatcaca aatgttgata gtttgaaaga aacattagaa cctttggtca
     2341 aatcacatca ggccctgatt catagcatgg ctgtatccga ttatacacct gtttatatgg
     2401 ctggtttaga tgaaataaaa gcaactgaag acatttctag tctactaaaa aaacagaata
     2461 cagaaagtaa gatttcatcc aaggatgact accaagttct tttcttgaaa aaaactccca
     2521 aggtcatttc ctatgtaaaa aaatggaatc cagctatcac actctttggc ttcaaactat
     2581 tggttaatgt tcctaaggag gaattatttg ccgtagctcg ccaaagcatt gaacgaaatg
     2641 gcgctgatta tatacttgct aatgacttga aggatattgg agaaaatcaa cacattgcct
     2701 atttagttga taaaacaagg gagattcaag cacagacaaa agatgaaatt gctcagctca
     2761 ttttaaatac cctagaaaaa ggagaacaaa atggctaata tcacacttgc cgttaccggc
     2821 tctatttcag cctacaaagc agctgaccta actagtcaat tgaccaagct cggtcatcag
     2881 gtcacagtcc tcatgagtcg ctctgctatg gacttcatca cacctttgac tctccaatcc
     2941 ctgtcaaaaa atctggtcca cacagatgtc atgattgaag aaaatcctac tcttatcaag
     3001 catatcgata ttgccaaaga aacagacgta tttttagttg cccctgcttc tgccaatacg
     3061 atagctaaat tagctaatgg tatggctgac aacattatta ctgcaacagc tctggctctg
     3121 ccaattggta ctaaaaaact gctggcacct gctatgaata ctaacatgta cctcaatcct
     3181 gctacccaga aaaatatcaa aaccctcatg gagtatagtt ttgaggaaat caaaccgcgt
     3241 gaagccctgc ttgcttgcgg cgactttggt acaggtgctc tggccgagat tgatgtgatt
     3301 ctaaacaaag taatggaggt tctaaatgaa aacaaataaa tccagggacg ttgcgactct
     3361 tgcaatattt ctaacaatca tgattgtcat tgaagtggtc agtcagatga tttttgctaa
     3421 ttttatcttg ccaataaaac cgactattac ccatattccc atcatcatcg cttccattct
     3481 ttacggtcca cgcttagggg cacaactggg tggttttatg ggaataatga gtatcattcg
     3541 caatagcata atcatttcac cgatgagtta tgttttctct cctttcgtag aacatggaaa
     3601 cttcaattct atcctgattg ccttagttcc acgtatttta atcggagtta caccatattt
     3661 tgtttacaaa ttgatgaaaa acaaaaaggg gctcctacta gcaggtctta ctggtacctt
     3721 gacaaataca atttttgtct tgactggtat cttcttccta ttttctaatg tatatgctgg
     3781 ggatataaaa ttgctactag ctagtatcgt ctcattcaat tctattgcag aattaaccat
     3841 ctccggtctc ttaaccatag ctatcgttcc agttttacag aaagttcaaa aataaatatc
     3901 agaaaatcat gtgaaagcgt gatttttctt ttcaaacatg gtataatgaa agcgtttaat
     3961 agaaaaattt ttaaggagat atgcgatgac ttatcaagaa acctatcaaa catggctcga
     4021 ctttgcggac cttccagatt acttgcgtga agaattagtc gcaatggatg aaaaaacaaa
     4081 agaagatgcc ttctatacaa atcttgaatt tggtacagct ggtatgcgtg gttatattgg
     4141 cgctggtacc aaccgcatca acgtattcgt agtccgtcaa gctactgaag gtttggcaaa
     4201 attggtagaa tcaaaaggtg aagaagctaa aaaacgtggt gttgccatcg cttacgactc
     4261 acgtcacttc tcgccagaat ttgcttttga atcagctcaa gttttggcag cgcatggtat
     4321 caaatcttat gtatttgaaa gccttcgtcc aactcctgag ttgtcattcg ctgtgcgcca
     4381 ttacaatacc atcgcaggta tcatggtgac tgcaagccac aacccgaaag aatttaacgg
     4441 ctacaaggtt tacggtgaag acggcggaca aatgccacca gctgatgcag atgcattgac
     4501 caacttcatc cgtgccatcg acaatccatt tgtagttgaa ttggctgacc ttgaagccag
     4561 caaggaaaat ggcttgatta cagttcttgg cgaagaaact gacgttaaat atcttgaaga
     4621 actcaaagac ctcaacatca accctgaatt gattgctgaa tacggtaaag acatgaagat
     4681 tgtctacaca ccacttcacg gtacgggtga aatgttggcg cgtcgtgccc ttgcacaggc
     4741 tggttttgaa tctgttcaag ttgtggaagc acaagcaact gctgaccctg acttctctac
     4801 tgtcgcttca ccaaacccag aaagccaagc agcctttgcc cttgcggaag aattgggtcg
     4861 ccaagtcggg gcagatgtcc ttcttgcaac tgaccctgac gctgaccgtg ttggtgttga
     4921 agttcgtcaa gctgacggtt catactggaa cctttctggt aaccaaatcg gtgctatcat
     4981 tgctaagtac atccttgaag ccaacaagca agcaggtaca cttccagcaa acgctgccct
     5041 tgctaagtca attgtatcta ctgagttggt aactaagatt gctgaaagct atggcgctac
     5101 catgttcaac gtcttgactg gtttcaaatt catcgctgag aaaatccaag agttcgaaga
     5161 aaagcacaac catacttaca tgtttggttt tgaagaaagc tttggttatc tcatcaagcc
     5221 attcgtacgt gacaaggatg ctatccaggc tgtgcttatg gttgctgaaa ttgctgccta
     5281 ctaccgttca cgtggcatga ccttagctga cggtatcgat gaaatcttca aggaatacgg
     5341 ctacttcgct gagaaaacaa tttctgttac actttctggt aaggatggtg cagagcaaat
     5401 caaggcaatc atggctaaat tccgcgataa ctcaccagca caattcaacg cgacggacat
     5461 cgcggtcttt gaagactttg cccttcaaac taagacagat aaagatggaa acgttgaaaa
     5521 gctcactact cctccttcag atgtcttgaa atacaccttg gcagatgatt cttggtttgc
     5581 agtccgccct tcaggaacag agccaaaaat caaattctac attgcaacag tcggcgaaac
     5641 ccttgctgaa gcagaagaaa agatcgccaa tatcgaagca gaaatcaacg catttgttgg
     5701 ataatcattt aaaaccaaaa agagtcgctt tttttcgcgg ctcttttttt gtttctattt
     5761 ttccgttagt acctatttat aggacaatat tttatagttg aatgaatttc tttaaatcaa
     5821 agaactgagg ggcattacac ttttttagac cacgaaaatc cgaaagtgac gatgaatcat
     5881 aaccaattca aacgacaaaa tttttcagct ctaaagttac atactaggcg ttcaatcatt
     5941 gctacttcac tttctcaatg tatgaacggt aacagaaaac ttttcattcc ctgcatttgt
     6001 ccactttctg tctttactat ttcaaaacca aattttctat aaaaattaag tgctggggta
     6061 tttccagtca atacttcaat tgaaataggt tcatgcatct ttttgagaac cacattcact
     6121 aaagccgttc caatcccgtg gcggctatat tccctatcta catataacca tgctagttca
     6181 tcatctgtaa atgcaacaaa tccgactaca aatccttcca tctctgccac aagaatctgg
     6241 taatcaaaca atccttccct atcagtagca atactaagcg gaataaatgc ttcagataag
     6301 ttagctaaat ccaatttttc ttgtcgtgct gggtcatgaa tttgacaaag cctttcaaag
     6361 tcttcttgaa gatactgtct aatataaact gtcgtttcaa tccatatcgg ctattctagg
     6421 actaagatga aaaaatctgt aaatgcacag aaaaaaattg accccgcaaa tctcccaaaa
     6481 ataatggtga gccatgtact tgaattattc caaaaaaaat actcctctgg agcagtgaga
     6541 caaattggtg tgtcttatag tggtttcgta gatgaaaact atactatact ctactatcac
     6601 tctttgataa tgtagaacaa attgaaaaag agaatagact tcagacagct attgatgtgg
     6661 tccgagaaca gtttggtttt ttagccatac aaaaaggaac cgtcctaact gaaggttcca
     6721 gaaatattga acgcagtaaa cttatcggcg gtcattccgc tgttggattg gagggattaa
     6781 aatgattgac cgttcatact taccatttca gtcagcaaga gagtaccagg acactaagat
     6841 gcaaaaatgg atgggctttt tcctatctga gcatacatca ccactctccg atgatacaaa
     6901 caaagtaacg tacatatctg atttatcact agagaagaaa ttattactcc tcagtcaagt
     6961 atacactggg cagctacgca cactcattca agtgattgaa aaaacaagcg tgtttcctac
     7021 attggagcaa taccaagtct gaataaggat ttcattttga caaaaactac aacaggtcac
     7081 atcaatttaa aattgaaaga tattatgagt attgaacttg ttgaggagga gctttatgaa
     7141 tcagcttgag tttcagcgta atcacctgca aatggactat tacagcgata gttaccaaga
     7201 ttttgaacgt gacttctacc gctattccaa catgaatatt ccattgacct tcctgaccga
     7261 cgatatcctc aaaacaatgg cgacttcatg taagaattac tttgtcctca ataaggaaaa
     7321 gtccaaagat aaccgcgatc acttcttcat atttgaagta agtaccgtag atgagaatcc
     7381 tctaatctac cgctattctt ataagaaaac tacaacgaat ttaacagaaa aataggagca
     7441 gttcaattga ctgttcctat ttttaatctt cataaaatct aagctattct attttgaaaa
     7501 atttaccatc ttcgaatatt tttgccaaag gaccagatgg tttaaaaaag aattccgtat
     7561 caatttatct ctatgagtaa tccattttcc ttacagttta ccaccgcttg ttgcaatccc
     7621 ttcaataaat tgtttttgga aaatgataaa taaaacaatc atcggccaaa tagctaataa
     7681 agaccctgcc atcagctgcg gatagttggt tgaatactgt ccttgtaaat tggctaaacc
     7741 agcagctaaa ttcatcttat caatagacgt attcactatc aatggccaca ttaaatcttt
     7801 aaatgcaaat aatgctgtaa aaatggataa agcaatcaac cctgatcgtg ctagtggaag
     7861 catgatttta aagaagattg tccagatatt tgcaccatcc aagtaggccg cttcttcaag
     7921 ttcttttggt aagccaagaa aaaattgtct aagtaaaaac gtaccaaatg cactgacgat
     7981 tcctggaaca acaagtgccg gaactgtatt tagtaatcct attttttgaa caagtagata
     8041 ttgaggagtt ataaaaattg aacctggaat catcatttgg accacgacta ttccaaaaaa
     8101 tattcttttt ccggggaact ccaaacgcgc tgttgcatat gcagccattg ccgaaaaaag
     8161 aaccgatcct gccacacgac caacaaccat caaaaatgta ttgaagtaaa aggccataaa
     8221 agggagtaaa tttaatactt cagtatagtt cgaaacttga acagttttag gaaagattgt
     8281 tggcggaata gctgtagctt cttgtagtgt tttccctgaa gttagtagca tccataggaa
     8341 gggtaagatc atactaagtg ctccaaaaga aagaataaga tgtactaacc attgctttct
     8401 cttcatcata ttattccttt caataatgta cccacatttt ttgaagtttc atctgtacag
     8461 cagtaatcat aagtataatc aacaagagaa aaactactat cgctgaacca tatcctttat
     8521 catttaaaat aaaggaatgt ttataaaata agtaagcaag tgattgggtt ttatacattg
     8581 cctcgctaga cggaggcatc atcataaaaa tcacatcgaa tacttggaaa gaa
//
LOCUS       DATUCG010000106         8557 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.108, whole genome shotgun sequence.
ACCESSION   DATUCG010000106 DATUCG010000000
VERSION     DATUCG010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8557
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.108"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            62..135
                     /locus_tag="VCA20_001866"
     tRNA            62..135
                     /locus_tag="VCA20_001866"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:96..98,aa:Pro,seq:tgg)
     gene            148..221
                     /locus_tag="VCA20_001867"
     tRNA            148..221
                     /locus_tag="VCA20_001867"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:182..184,aa:Met,seq:cat)
     gene            238..311
                     /locus_tag="VCA20_001868"
     tRNA            238..311
                     /locus_tag="VCA20_001868"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:272..274,aa:Ile,seq:cat)
     gene            318..407
                     /locus_tag="VCA20_001869"
     tRNA            318..407
                     /locus_tag="VCA20_001869"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:354..356,aa:Ser,seq:tga)
     gene            418..491
                     /locus_tag="VCA20_001870"
     tRNA            418..491
                     /locus_tag="VCA20_001870"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:452..454,aa:Met,seq:cat)
     gene            507..579
                     /locus_tag="VCA20_001871"
     tRNA            507..579
                     /locus_tag="VCA20_001871"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:540..542,aa:Phe,seq:gaa)
     gene            611..684
                     /locus_tag="VCA20_001872"
     tRNA            611..684
                     /locus_tag="VCA20_001872"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:645..647,aa:Ile,seq:gat)
     gene            697..784
                     /locus_tag="VCA20_001873"
     tRNA            697..784
                     /locus_tag="VCA20_001873"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:731..733,aa:Ser,seq:gct)
     gene            844..1680
                     /gene="mreC"
                     /locus_tag="VCA20_001874"
     CDS             844..1680
                     /gene="mreC"
                     /locus_tag="VCA20_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEP1835636.1"
                     /translation="MNKFSKLVVVVSIFLLLSFSLLFVTFSKGLQVPYLNNIVRVVVT
                     PIQSVISVPTRFFSEQKDVLTDLMNAYEENKQLKEAIMSLEEMAAENTSLKEENASLR
                     SSLGVVSDFPEKQLIPGSVLVRTPSSWSEHISINIGETSGVTSNALVVANGGLVGIVS
                     SLSSDSAVVTLFTNSDEFTKLPVKISVDSKEIYGILSGYDADTNSFIINQLNSADEIA
                     VGSNVVTSDLAGATPANVQIGKVLSVKSNSNSLNREVYVEPTASFSNIYSVLVVGQTN
                     AQ"
     gene            1670..2185
                     /gene="mreD"
                     /locus_tag="VCA20_001875"
     CDS             1670..2185
                     /gene="mreD"
                     /locus_tag="VCA20_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEP1835637.1"
                     /translation="MRNKMIEIFMFPILFFILLLDGQISTLVTNWSVGLFTISSHLVL
                     MLAIFYANYVSLGFSLFIFTLLGLVYDISYLNLIGIATTTLPLVLYCIYFFFQGAVSK
                     RGINILILLVAIFQFEFISYSFARIFHITNLSVFIFVFNKLLPSLLFNLALFFVLQPL
                     FERLFGITNKT"
     gene            2270..3526
                     /locus_tag="VCA20_001876"
     CDS             2270..3526
                     /locus_tag="VCA20_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEP1835638.1"
                     /translation="MKKKILATIMLSTVVLSNANYVAVISANDVDSQIAAKNQQISEL
                     TAQQAEAQQQVDTIQGQVDAIVSEQAKLTEENTRLEAESQTLAADIERLSADIVSRDG
                     ALKEQARSAQVDGSASSYINTILDSKSIVDAVSRVNAMREIVSANNRMLEQQKADKEA
                     IVEKQKANQEAITTLAANRQKLEDDAQVLQVRQAELEAAKLNLAVQKATAEDEKNSLL
                     AQKAAAEEAARQAAARQAEYQAQQAALAQQQVASVSAPVVSTPVETTATETVAAPTQT
                     VSQSTPTVSTPTTSTSSGSGSSAAANNARYDASSYPVGECTWGVKSQLSWVGPYWGDA
                     KQWLASARAEGFSTGSTPQVGAIAVWTGGYYGHVAVVTAVQSSTSIQVVESNYMGRRY
                     IGNHRGGYFNPTTTSEGAVYYIYPPY"
     gene            3629..4597
                     /locus_tag="VCA20_001877"
     CDS             3629..4597
                     /locus_tag="VCA20_001877"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEP1835639.1"
                     /translation="MAFTDLKLFALSSNQKLAEQVSKKIGIPLGKSSVRQFSDGEIQV
                     NIEESIRGTHVYILQSTSSPVNDNLMEILIMVDALKRASAESVNVVMPYYGYARQDRK
                     ARAREPITSKLVANMLQTAGVNRLLTIDLHAAQIQGFFDIPVDHLMGAPLIADYFERR
                     GMVGDGYVVVSPDHGGVTRARKLAQFLKTPIAIIDKRRSVDKMNTSEVMNIIGDIKDK
                     TCILIDDMIDTAGTICHAADALADAGATAVYASCTHPVLSGPAMDNISKSAIKKLVVL
                     DTIEIAEDRLIDKIEHISTAELLAEAIIRIHEKRPLSPLFEASRVK"
     gene            4684..5862
                     /locus_tag="VCA20_001878"
     CDS             4684..5862
                     /locus_tag="VCA20_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEP1835640.1"
                     /translation="MDLLNRFNKNLNRIEVSMIRQFDQSISDVPGILKLTLGEPDFTT
                     PAHVKEAAKAAIDADQSYYTGMAGLLELRQAAAEFVAEKYNLHYNPDTEILSTIGATE
                     ALSASLVAILEAGDTVLLPAPAYPGYEPIVNMVGADIVEIDTTANDFVLTPEMLEEAI
                     IEQGDKLKAVILNYPANPTGVTYSREQIQAFADVLRNYPVFVLSDEVYAELTYTGQPH
                     TSIAEFLPEQTILIQGLSKSHAMTGWRIGLIMSQAPIIAQIIKSHQYLVTAASTAMQY
                     GAVEALKNGKDDALPMRAEYVKRRDYIIEKMTDLGFKIIKPDGAFYIFAKIPAGYNQD
                     SFSFLQDFARKKAVALIPGAAFGQYGEGYVRISYAASMEKIQTAMARLKEYLEENGTN
                     "
     gene            5849..6631
                     /gene="recO"
                     /locus_tag="VCA20_001879"
     CDS             5849..6631
                     /gene="recO"
                     /locus_tag="VCA20_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEP1835641.1"
                     /translation="MERIETRGLVLYNRNFREDDKLVKIFTEKAGKRMFFVKHASKSK
                     LVASIQPLTYADFIVKINDDGLSYIEDFHQVQPFKSINGDIFKLSYATYILALADAAL
                     QDKVYDPALFAFLVKTLDLMESGLDYEVLTNIFEIQLLGRFGISLNFHECAFCHRVGL
                     PFDYSYKYSGVLCPQHYQQDERRAYLDPNVPYLLDQFQAISFDELETISIKPEMKRKL
                     RFFIDQLYEEYVGIHLKSKKFIDDLSSWGQIMKPRTENEETE"
     gene            6628..7635
                     /gene="plsX"
                     /locus_tag="VCA20_001880"
     CDS             6628..7635
                     /gene="plsX"
                     /locus_tag="VCA20_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEP1835642.1"
                     /translation="MKRIAVDAMGGDHAPQAVVEGVNQALAAFPDIEIQLYGDEAKIK
                     QYLTVTERVSIVHTTEKINSDDEPVKAIRRKKDASMVLATKAVKDGQADAVLSAGNTG
                     ALLAAGVFVVGRIKNIDRPGLMSTLPTMDGKGFDMMDLGANAENTAHHLYQYGILGSF
                     YAEHVRGVKQPRVGLLNNGTEDTKGTPVHQETYKLLAEDKSINFIGNVEARELLNSVA
                     DVVVTDGFTGNAVLKTIEGTAKSIVGQLTGSIKNGGLRAKLGGLLVKPTLKKALGAMD
                     YKTAGGAVLLGLKAPVIKAHGSSDAQSIFYTIKQTRSILEAGIVEKSVAKFSVVEESH
                     D"
     gene            7628..7876
                     /locus_tag="VCA20_001881"
     CDS             7628..7876
                     /locus_tag="VCA20_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEP1835643.1"
                     /translation="MTREQVYQRVVELIQDEKGEDFQVQPESTLADNIAADSVEIMEF
                     VLNLEDEFHVDVPDAAIEHFEVLSDIVDFIYEEAKKRS"
     gene            7994..>8557
                     /locus_tag="VCA20_001882"
     CDS             7994..>8557
                     /locus_tag="VCA20_001882"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEP1835644.1"
                     /translation="MKTDLLYSGKAKDIYATADSDQIVAVYKDQATAFNGGKKEQIVG
                     KGRLNNLISSLIFEKLNEAGVPTHFIKRLSDSEQLNKKVEIIPLEVVLRNVTAGSFSK
                     RFGVEEGITLPTPIVEFYYKKDELDDPFINDEHIVFLELASQDQIAYIKEETRRINGF
                     LKDLFTQIGLTLVDFKLEFGVDSSGQIL"
BASE COUNT         2362 a         1507 c         2045 g         2643 t
ORIGIN      
        1 tcctctaggg tgcatcactc gcttaatgga gacgttaggg gagctttatt tttaatagta
       61 tcgggaagta gctcagcttg gtagagtact tggtttggga ccaaggtgtc gcaggttcga
      121 atcctgtctt cccgattata ttattttggc ggtgtagctc agctggctag agcgtccggt
      181 tcatacccgg gaggtcgggg gttcgatccc cttcgccgct atatactctt gtttgctgga
      241 cctttagctc agctggttag agctctcggc tcataaccga gcggtcgtag gttcaagtcc
      301 tacaaggtcc atatattgga ggattaccca agtccggctg aagggaacgg tcttgaaaac
      361 cgtcaggcgt gtaaaagcgt gcgtgggttc gaatcccaca tcctcctttt ctactaccgc
      421 gggatggagc agctaggtag ctcgtcgggc tcataacccg aaggtcgtag gttcaaatcc
      481 tgctcccgca atttctttaa gaacttggct cggtagctca gttggtagag caatggattg
      541 aagctccatg tgtcggcggt tcgattccgt ctcgcgccat taatttaact ttgtccaagt
      601 ttattatctt gggcgcgtag ctcagatggt tagagcgcac gcctgataag cgtgaggtcg
      661 gtggttcgat tccactcgtg cccatattat attgatggag aattactcaa gaggctgaag
      721 aggacggttt gctaaatcgt taggtcgggt aactggcgca agggttcgaa tcccttattc
      781 tccgtagtga acgagattat cgcttgatat tctcgtttta ttgtatctta gtgaggatat
      841 attatgaata aattttcaaa attagtagta gttgtttcta tctttttatt actgtcattt
      901 tctcttttgt ttgtgacttt ctctaagggg ttacaggtac catatttaaa taatatcgtt
      961 agggttgttg taacgccaat tcaatcggtt atttcagtac ctactagatt tttttctgag
     1021 cagaaagatg tcttgacaga tttgatgaac gcttacgagg aaaataaaca attaaaggaa
     1081 gctattatga gccttgaaga gatggctgct gaaaatacta gcttgaaaga agagaatgca
     1141 tcgcttcgta gcagtttagg tgtggtatct gattttcctg aaaaacaact tattccggga
     1201 tcagttttag tgaggactcc ttcatcgtgg tcggagcata tttcaattaa tattggtgag
     1261 actagtggtg tgacatctaa tgcacttgtg gttgctaatg gtggattagt tggcatcgtg
     1321 agttcattga gttcagattc ggcggttgtt accttattta cgaattcgga tgaattcacg
     1381 aagttgcctg tgaaaatttc tgttgattct aaagaaattt atggtatttt atcgggctat
     1441 gatgcagata caaatagttt tatcattaat cagttgaact cagctgatga aattgcagtg
     1501 ggtagcaatg ttgtaacgag tgatttggct ggtgcgactc cggcaaatgt ccaaattggt
     1561 aaagttttat cggttaaatc aaatagtaac agtttaaata gagaagtata tgttgagccg
     1621 acagctagtt tttcaaatat ttattcggtt ttagtggtag gtcaaacaaa tgcgcaataa
     1681 aatgatagaa atattcatgt ttcccatctt gttctttatt ttattacttg atggacagat
     1741 ttcaacgttg gtaacaaatt ggtcggtcgg attatttact atttcaagtc atttggtact
     1801 tatgttagct attttttatg ctaactatgt atctcttggt ttttcattat tcatatttac
     1861 tttactaggt ttggtatatg atattagtta tcttaatctg attggtattg ctactacaac
     1921 tcttccttta gttttgtatt gcatctattt cttctttcaa ggtgctgtca gcaagcgagg
     1981 aattaatatt ttgattttac tagtagctat ttttcaattt gaatttatta gttactcatt
     2041 tgctcgcatt tttcatataa caaacttgtc tgtgtttatc tttgttttta ataaattact
     2101 tccaagtcta ctattcaatt tggccttatt tttcgtacta caaccgttat ttgagcgttt
     2161 atttggaata acaaacaaga catagaaatg taataattgc gtaatatact tacgcaattt
     2221 ttttgataaa atgagagagt cagaaagtat agaggagtta tgttcggata tgaagaaaaa
     2281 aatcttggct acaattatgt taagtacagt cgttctatct aatgctaatt atgtagctgt
     2341 gattagtgcg aatgatgtag atagtcagat tgcagcaaaa aatcaacaga ttagtgagtt
     2401 gacagcacaa caagctgaag ctcaacaaca agttgatact attcaaggac aagttgatgc
     2461 aattgttagt gaacaggcga aattaacaga agaaaatact cgtttggaag cagaatcgca
     2521 gacattggcg gcagatattg agcgtttgtc agctgatatt gtgtcacgtg atggtgcttt
     2581 aaaggagcag gctcgtagtg ctcaagttga tggctctgct tcaagttata ttaatacaat
     2641 tttagattca aaatcaatcg ttgatgctgt ttctcgtgtt aatgcaatgc gtgagattgt
     2701 ttcagctaat aaccgtatgt tggaacaaca aaaagctgat aaggaagcaa ttgttgaaaa
     2761 acaaaaggca aaccaagagg caatcactac tttggcagct aatcgccaaa aattggaaga
     2821 tgacgcccaa gtattgcaag tgcgtcaggc tgaattggaa gctgctaagt taaatttggc
     2881 tgtgcagaaa gctactgctg aagatgagaa aaattcattg ttggcgcaaa aggcagctgc
     2941 agaagaagca gctcgtcaag cggcagctcg tcaagcggag tatcaagcac aacaggcagc
     3001 tctagcacag caacaagtag cttcagtatc agcaccagtc gtgtcaacgc cagtagaaac
     3061 tacagcgact gaaacggttg cagcccctac tcagacagta tcacaatcta caccaacggt
     3121 aagcacacct acaacgtcta catcatcagg ttcaggtagt tcagcggcag caaataatgc
     3181 gcgttatgac gcttcatctt acccagttgg tgagtgtact tggggagtta aatctcaact
     3241 ttcatgggtt ggtccttact ggggagatgc taaacagtgg ttagcatcag cacgtgctga
     3301 aggttttagt acaggttcta ctccacaagt aggtgcgatt gctgtttgga caggtggtta
     3361 ttatgggcac gtagcggttg ttacggctgt tcaatcttca acaagcatcc aagttgttga
     3421 atcaaactac atgggtcgtc gttatattgg taaccatcgt ggtggttatt tcaatccaac
     3481 aacgacttct gagggagcgg tttactacat ttaccctcca tattaagaaa ttttcaaata
     3541 gagcgtgtgg atttgcgctc tatttttgtt tgaaaaatga aggaaaaagg gttaaaatag
     3601 taaaggtaga aatatatagg aggctgtcat ggcgtttact gacttaaaat tgttcgctct
     3661 ttcgtcaaat cagaagttag ctgaacaggt gtcgaaaaaa ataggaattc ctctgggaaa
     3721 atcaagtgtt cgtcaatttt cagatggtga gattcaggta aatattgagg aatccattcg
     3781 tggaactcat gtgtatattc ttcaatccac tagctcacca gttaatgata atttaatgga
     3841 aattttgatt atggtcgatg ctcttaaacg tgcatctgct gaatcagtaa acgtagtaat
     3901 gccttattat ggttatgcac gccaagaccg gaaggctcgt gcacgggaac caattacatc
     3961 aaaattggtg gctaatatgt tgcagacagc gggggttaat cgtttgttga caattgattt
     4021 gcatgctgcg cagattcagg gattcttcga tattcctgtt gatcatttga tgggtgctcc
     4081 attgattgcg gattattttg agcgtcgtgg tatggtaggt gatggctatg ttgttgtatc
     4141 ccctgaccat ggtggtgtga cacgtgcccg taagttagca cagttcttga agacaccgat
     4201 tgctattatt gataaacgcc gtagtgttga taagatgaat acatcagaag tgatgaacat
     4261 tattggcgat attaaggata aaacttgtat cttgattgat gatatgattg atactgcggg
     4321 taccatctgt catgcagcgg atgctttggc agatgcaggg gcaacagctg tttatgcttc
     4381 atgtacgcat ccagtattgt cagggcctgc tatggataac attagtaagt cagcaattaa
     4441 gaaattggtt gttcttgata caattgaaat tgcagaagat cgcttaatcg ataaaatcga
     4501 acatatttca acggcagaat tattagcgga agcgattatt cgtattcatg aaaaacgtcc
     4561 tttgtcacct ttgtttgagg caagtcgtgt taaataggta tgagaagagt caaatgactc
     4621 ttctttttta catttatttt caaaatgtat tataatagtt ttatatgttt ttgaggtagc
     4681 agaatggatt tattaaatcg ttttaataag aatttgaatc ggattgaggt ttcgatgatt
     4741 cgtcagtttg atcagtctat ttcggatgtt ccagggattt tgaaactgac cttgggagag
     4801 cctgatttta caacgcctgc tcatgttaaa gaagcagcta aggctgctat cgatgctgat
     4861 cagagttatt atactggaat ggctggtctt ttggaattgc gtcaggctgc agctgagttt
     4921 gtggctgaaa aatataattt acactacaat ccagatacag aaattctatc tacaatcggt
     4981 gcgacagagg ctttatcagc gagtttagtt gctattttgg aggctggaga tacggttctc
     5041 ttgcctgccc ctgcctatcc tggttatgag ccgattgtca atatggtggg agcagacatc
     5101 gttgaaatcg atacgacggc aaatgatttt gtattgacac ctgagatgtt ggaagaggcg
     5161 attattgagc agggggataa gctaaaagct gtcattctca actatcctgc caatccaacg
     5221 ggagtgacct actcacgtga gcagattcag gcctttgcgg atgtcttgcg taactatcct
     5281 gtctttgtcc tatcagatga agtttatgcg gagttgactt acactggtca gcctcatact
     5341 tcgattgcgg agtttttgcc agaacagacc attttgattc agggcttgtc caagtcccat
     5401 gctatgacag gttggaggat tggcttgatt atgagtcagg ctcctattat cgctcaaatc
     5461 atcaagagcc atcagtatct ggtaacagcg gcgtcaactg ctatgcagta tggtgcggtt
     5521 gaggccttga aaaacggtaa ggatgacgct cttccgatgc gtgcggagta cgtcaagcgt
     5581 cgggattata tcatcgagaa gatgacggac ttgggcttta aaattatcaa gcctgatgga
     5641 gcattttata tctttgcaaa aatccctgcg ggttacaatc aagattcctt tagcttcttg
     5701 caagactttg cgagaaagaa agcggtggcc ttaattccgg gtgcggcctt tggtcagtat
     5761 ggtgaaggct atgtgcgaat ttcctacgca gcaagtatgg agaagattca aacagccatg
     5821 gctcgtttga aggaatatct agaggaaaat ggaacgaatt gaaaccaggg gattagtcct
     5881 atataatcgg aattttcgag aagatgacaa gctggtcaag atttttacag agaaggctgg
     5941 caagcgaatg tttttcgtga aacatgcctc taaatccaag ctggtagctt ctatccagcc
     6001 tttgacctat gcggatttta tcgttaaaat caatgatgat ggtctgtctt atatcgaaga
     6061 ttttcatcag gtacagccct ttaagagtat taacggtgat attttcaagc ttagctatgc
     6121 tacctacatc ttggccttgg cagatgcggc cttgcaggac aaggtctatg acccagctct
     6181 ctttgctttt ttggtcaaga ccttggactt gatggagtca ggtttggact acgaagttct
     6241 gaccaatatc tttgaaattc agctcttggg tcgatttggg atcagtctga attttcacga
     6301 gtgtgctttt tgtcatcggg ttggcttgcc ttttgactat tcctacaagt acagcggtgt
     6361 cttgtgtccg caacactatc aacaagatga gcgacgggct tatctggatc ccaatgttcc
     6421 ctatctactt gatcaatttc aagctatttc ctttgatgag ctggaaacca tttccatcaa
     6481 gcctgagatg aagcgaaaat tacggttttt tattgaccag ctgtacgagg aatatgtggg
     6541 gattcacttg aaatccaaga aatttataga tgatttgtct tcttgggggc agattatgaa
     6601 accaagaaca gaaaatgagg aaacagaatg aaacgtattg cagtagatgc tatgggtggg
     6661 gaccacgccc ctcaggcagt ggtagaaggt gtcaatcaag ccttggctgc ctttccagac
     6721 attgaaattc aactttatgg tgatgaggct aaaatcaagc agtatttgac agtgacagag
     6781 cgtgtcagca tcgtccatac tacggagaaa atcaattcag atgatgagcc tgtcaaggct
     6841 attcgtcgta agaaagatgc ttcgatggtc ctagcgacca aggctgtcaa agatggtcag
     6901 gcagatgcgg tcttgtcagc tggaaataca ggtgctcttt tggcggcagg cgtctttgtc
     6961 gtcggtcgca tcaagaacat cgaccgtcct ggtcttatgt ctaccttgcc gactatggac
     7021 ggtaagggat ttgatatgat ggatttgggg gcaaatgctg aaaatacagc tcatcacctc
     7081 tatcagtatg gtattcttgg ctcattttac gcggagcacg tgcgtggtgt caaacaacct
     7141 cgtgtgggac ttttgaacaa cggtacggaa gataccaagg gcacgccagt tcaccaagaa
     7201 acctataaac tcttggcgga agacaagtca atcaacttta tcggtaatgt ggaggcgcgt
     7261 gagttgctca acagtgtggc agatgtggtc gtgacggatg gtttcacggg aaacgctgtg
     7321 ctgaaaacca ttgagggaac tgcaaaatcc atcgttggtc agttgactgg ctcaatcaag
     7381 aatggcggtc tgcgtgctaa attgggaggc ttgttggtca aaccaacctt gaaaaaggcc
     7441 ttgggggcta tggactataa aactgcaggt ggtgctgtct tgcttggtct gaaagcacct
     7501 gtcatcaagg cgcacggatc cagcgatgcc cagtcgattt tctatacgat taagcagacg
     7561 cgttcgattt tggaggctgg cattgttgaa aaatcagtgg ccaaattttc agtagtggag
     7621 gagagtcatg actagagagc aggtctatca gcgggttgtt gaattaattc aggatgaaaa
     7681 gggagaagat tttcaagttc aacctgaatc tactttggca gataatattg cagcagattc
     7741 agtagaaatc atggaatttg ttcttaattt ggaggatgaa tttcatgtag atgttccaga
     7801 tgctgctatt gagcattttg aggtcctatc tgatattgtt gattttattt atgaagaagc
     7861 aaagaaacgt tcgtagttta cgggcgtttt tctttgtttt ttcctaaaaa accgtaaatt
     7921 tgttgtgaat gcgaatattg tgtgttagaa taaacgaaaa gactagaata ttttacgaaa
     7981 ggcgaacatt ctcatgaaaa cagaccttct ctattcagga aaagccaaag acatctacgc
     8041 tacggctgat agcgaccaga ttgttgcggt ttataaggat caggcaacgg cttttaatgg
     8101 tggtaagaaa gaacagattg tgggcaaggg ccggctcaat aatctgattt catctttgat
     8161 ttttgaaaaa ttgaatgaag ctggtgtccc aacgcatttt atcaagcgtt tgtcggattc
     8221 ggagcagttg aataagaagg tggagattat tcctcttgag gttgttttgc gaaatgtgac
     8281 agctgggtct ttctcaaaac gcttcggtgt ggaagaaggg ataaccttac ctactcctat
     8341 cgtggaattt tactataaaa aagatgagtt ggacgatcct tttatcaacg atgaacacat
     8401 tgtctttcta gaactggcta gtcaggacca aatcgcctat atcaaggaag aaacaagacg
     8461 aatcaatggg ttcttgaagg acttgtttac tcagattggg ttgactttgg tggacttcaa
     8521 gctagaattt ggggttgact cgtctggtca gatttta
//
LOCUS       DATUCG010000107         8407 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.109, whole genome shotgun sequence.
ACCESSION   DATUCG010000107 DATUCG010000000
VERSION     DATUCG010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8407
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.109"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            12..296
                     /locus_tag="VCA20_001883"
     CDS             12..296
                     /locus_tag="VCA20_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEP1835645.1"
                     /translation="MKKFAKLVFAVGIACSLMASTVAFADFVDGGEWHYGVGWTGTYG
                     YSNYHHPTRSHTATVRNGQHENRQRQGAGIWAKASITKIPPTGMEYFYGF"
     gene            518..2626
                     /locus_tag="VCA20_001884"
     CDS             518..2626
                     /locus_tag="VCA20_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEP1835646.1"
                     /translation="MKRLNHFCMILLLCCVSFIVVNLHYTKEINRIRELGTTDYSFQF
                     YLRDSAVPSQDLLHFFEKIADKYDVSIVKTDSNTEVIKAGVFSHETFPYKDFGIQQLT
                     FNEDGSGSYTNIQSPDKLGHIPTFLQAKTIRLITLKNYFQDTSHTLNGQYTVTTTKSI
                     NDTEIVQELSHFFNIDSSTLLTPTFETAVELINRDLLLVALICCIAFLLLSLTSVYQP
                     ILDIKHIGVEKILGYSNLEIFYRYVKQNILIMGIGSIILNSLPFLFFDYIPQGFSAMM
                     LLAHFILLQLYLLINILVITIIQRINAASIIKGFVSFKTGFYINNFFKCCLTLLITIL
                     MIGVGNSMYEEHQELNYQEQWEKQGDFLTLETVKSGNQLWQDSLSGSDKAHQYYYSLY
                     KNLTENLPTYYIRSSIITPGNFSHLKEFEHSTLANDIPVLYANKTYLESIGFPIPHTS
                     KQLTVLFPASMKENEESNKNLAKLIGLLSLKYEEQVIKTISEVDVECIYYESDWTFFP
                     YNETLSNNFTNPIISLVNDNDMSWEDKAHLSNTGLSSPIKIANTPQNQQKIEQILQKL
                     PDDSYFKFSSIKSIQQGRVDSFRDASRNFITLFAIICLLSIVASYFIVRSMFLWRKSH
                     IFTMKFLGWSLLDRYKPILIGLALLYLLPLPVILIAGKSLFPLVLFLLFAIADGGIFL
                     FTSLSMEQKNLVQYLKGENL"
     gene            2623..3252
                     /locus_tag="VCA20_001885"
     CDS             2623..3252
                     /locus_tag="VCA20_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1835647.1"
                     /translation="MIHLEHIFKKIGQKHILHDFSLHIEQGELVAIVGKSGSGKTTLL
                     NIIGLLDGEYEGNYLLFGHSNVPVNTRTSQKIIREQISYLFQNFALIENESVEYNLLM
                     ALKYTRLSKKEKEESIKNILEKIGLANTLKQKISELSGGEQQRIAIARALLKPSNLIL
                     ADEPTGSLDQENRDLILQFLIEMNNRGKTVIIVTHDPYIADKCQRIVRL"
     gene            3413..3628
                     /locus_tag="VCA20_001886"
     CDS             3413..3628
                     /locus_tag="VCA20_001886"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835648.1"
                     /translation="MTVAFIIFLIIKVLALILFLACLFLGYKLVKTLNKKQVSPAKKW
                     GIIGLYSLFLLIVAYLATFVVVLGINT"
     gene            3665..3955
                     /locus_tag="VCA20_001887"
     CDS             3665..3955
                     /locus_tag="VCA20_001887"
                     /inference="COORDINATES: protein motif:HMM:NF020523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEP1835649.1"
                     /translation="MIDIKTNKTLLRQIETILNTQLLSVQELAILKHAQTALQHDEYL
                     LKVIAYLKQELSPLAISNSLTPEVSELFLEINRQHPATRAAAIWNFLVPKTK"
     gene            4221..4409
                     /locus_tag="VCA20_001888"
     CDS             4221..4409
                     /locus_tag="VCA20_001888"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835650.1"
                     /translation="MKPTNLFHKTSTVFTLIGLHLVVFLLSGLRSWMLHQPFKWWITL
                     GISVGFSVAYLLVTKNDS"
     gene            complement(4808..5167)
                     /locus_tag="VCA20_001889"
     CDS             complement(4808..5167)
                     /locus_tag="VCA20_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEP1835651.1"
                     /translation="MQRIFYLVFGFVSLVVGVIGIVLPIVPTTPLLLLAGFCFARSSK
                     RFEKWLRNTKIYQFYVADYVETKSISRKRKKQIIWQIYLLMAISIWLAPILLVKIGLG
                     LLTIFITYYLFWVIPEK"
     gene            5294..5896
                     /locus_tag="VCA20_001890"
     CDS             5294..5896
                     /locus_tag="VCA20_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007892530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEP1835652.1"
                     /translation="MKLLGLVGTNSARSTNRKLLQYIEQHFADKADIELVEIKELPIF
                     NKPANRELPEIVKELVAKIEAADGVIIGTPEYDHSIPAVLMNALAWVSYGVYPLLNKP
                     VMITGASYGTLGSSRAQLQLRQILNAPELKATVLPDEFLLSHSLQAFDANGELIDLET
                     SQKLDAIFDDFRLFVTMTSKLSNAKKLLQKEAENFDWENL"
     gene            5911..7146
                     /locus_tag="VCA20_001891"
     CDS             5911..7146
                     /locus_tag="VCA20_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003107773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEP1835653.1"
                     /translation="MKFVGIVGSNADQSYNRMLLQFIQRHFKVKFELELLEIKDVPMF
                     NQDEDQSDCFAIQYLYNKITRADGVIIATPEHNHTITPALKSTLEWLSFNLHPFENKP
                     VMIIGASYYDQGTSRTQVHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDDKGNIINEG
                     TVKFLESCLDNFIKYVGVVSKLKKPKPIAPEDLDCTKPIATTITGVDPDDPDWLEKAS
                     KLVNAVEGDTYVKLDNGLLTVNQLNMFLNAMPFELTYADDNNQFLYYNNSHQEPDTML
                     GKRVLAQVGNRLSTVHGTLPPARTKNVEWVVGTLRNGNQEYVRTIVPGTPAEIINTHN
                     YQAMYYPDGSYAGINEIIFNFKPWLDWYLEATGQRLVGGSGTAAAPAHVDATSGASDA
                     GSAPATSTPTDANSGASVH"
     gene            7430..8224
                     /locus_tag="VCA20_001892"
                     /pseudo
     CDS             7430..8224
                     /locus_tag="VCA20_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647491.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
BASE COUNT         2666 a         1750 c         1485 g         2506 t
ORIGIN      
        1 ggaaaattat catgaaaaag tttgcaaaat tagtttttgc ggtgggaatc gcttgtagtt
       61 taatggcatc cacagtcgcc tttgcagact ttgtcgatgg tggtgaatgg cattatggag
      121 ttggatggac aggaacctac ggatattcca actaccatca tccaacacgt tctcatactg
      181 ctactgtaag aaatgggcaa catgaaaacc gtcaacgcca aggtgcgggt atatgggcta
      241 aagcatcgat taccaaaatc ccaccaactg gtatggaata tttctatggt ttttgataaa
      301 cactaggtgt acactaaaaa gaaatcagga atccacaaat gatggaattg tgaaacaaac
      361 gattgccatg ctcagaaatg tttgatagta gggtaatcgg gaacaactgt tactcatcaa
      421 ttgctaaatg actgatagtc aaattcaaaa agatagttag tttccgacca gctctattgg
      481 tgtgctggtc ggaaaacgtt tataaggagg tgttcagatg aaaaggctaa accatttctg
      541 tatgattcta cttctgtgtt gtgtcagttt tatcgttgta aatctacact atactaagga
      601 aatcaatcgc atacgcgaat taggaacgac tgactattca tttcaatttt atttacgcga
      661 tagtgccgtt ccttcacagg atttactgca tttttttgaa aaaattgctg ataaatacga
      721 cgtttcaatt gtcaaaacag atagcaacac cgaggtaata aaagcaggtg ttttttctca
      781 tgagaccttt ccttataaag attttggaat acagcaactt acttttaacg aagacggaag
      841 tggatcttat acaaatatac aatcccccga caaactaggg catatcccaa catttttgca
      901 ggctaagacg atacggctca tcactttaaa aaactatttt caagatacat cccatacact
      961 aaatggtcaa tataccgtca caacaacaaa atcaataaac gatactgaaa tagtacagga
     1021 attgagtcat tttttcaata ttgattcttc tacactattg actccaacct ttgaaacagc
     1081 agtagaatta ataaatagag acttgctact agtagcactc atctgctgta tcgcattttt
     1141 actcctttca ttaacttctg tctatcaacc aattttagat atcaaacata tcggcgttga
     1201 aaaaatactt ggatacagta acttagagat tttctatcgc tatgtaaaac aaaacatact
     1261 aatcatgggg atcggtagta tcattctaaa cagtttacca tttctgtttt ttgactacat
     1321 tcctcaaggt ttttcagcta tgatgctgct agcacatttt atcctccttc aactctatct
     1381 actcatcaac attttagtca tcacaattat ccaacgcatt aatgcagcat ccatcataaa
     1441 aggatttgtt tccttcaaaa cagggttcta tatcaataat ttcttcaaat gctgtctcac
     1501 acttctcatc actattttga tgatcggtgt cggaaatagc atgtatgagg aacatcaaga
     1561 gttgaactat caagaacagt gggaaaaaca aggagatttc ctaacactcg aaaccgtaaa
     1621 atcaggaaat caactctggc aagatagcct gtctggttcc gacaaagctc atcaatatta
     1681 ttactcctta tataagaatc tgacagaaaa tcttccaact tactatattc gttctagtat
     1741 cataacccct ggcaatttct cacatcttaa agaatttgaa cattcaacat tggctaatga
     1801 catccctgtt ctatatgcca acaaaaccta tctagaaagc ataggatttc cgattccaca
     1861 tacaagtaaa caattgaccg tactctttcc agcatcaatg aaagagaatg aagagtctaa
     1921 caaaaatcta gcaaaattga ttggtttgct gtcactgaaa tatgaagaac aagtgataaa
     1981 aacaatcagc gaagtagatg ttgaatgcat ttattatgaa agtgattgga cattcttccc
     2041 ttacaatgaa accttatcca acaatttcac taatccaatc attagtttgg tcaatgacaa
     2101 cgacatgtcg tgggaagata aagcacacct gtcaaatact ggattaagta gcccaattaa
     2161 aattgcaaat acccctcaaa atcaacaaaa aatcgaacaa attcttcaga aattgcctga
     2221 tgatagctat ttcaaattct catcaataaa atctattcaa caaggacgtg tagatagttt
     2281 ccgcgatgct agtcgtaact tcatcacctt gtttgcgatt atctgcttac tgagcattgt
     2341 tgcttcctat tttatcgtga ggagtatgtt cctatggaga aaatctcata tcttcacaat
     2401 gaaattttta ggatggtcac tccttgaccg ctataaacca attctaattg gactagcatt
     2461 actttatttg ctacctcttc cagttatttt aatagctggt aagtcgctat ttcctcttgt
     2521 acttttttta ctatttgcca ttgctgatgg aggtatcttc ctatttacat cgctgagcat
     2581 ggagcaaaaa aatcttgtac aatacttgaa aggagagaac ttatgattca tttagaacat
     2641 atctttaaaa agattggtca aaaacatatc ctacatgatt tttccctcca cattgaacaa
     2701 ggtgaattag tcgcaattgt tggaaaaagt ggcagcggaa aaacaacatt actaaacatc
     2761 attggcttgc ttgacggtga atacgaagga aattatctcc tatttggtca ttcaaacgta
     2821 cctgtaaaca ctaggacatc tcaaaaaata attcgggaac aaatttccta tctattccaa
     2881 aattttgctc taattgagaa cgaaagcgtc gagtataatc tcctgatggc attaaaatat
     2941 accagattat caaaaaaaga aaaggaagag tctatcaaaa atatactaga aaaaatcgga
     3001 ctagcaaaca cgctaaaaca aaaaatttcc gagctgtctg gaggagaaca gcaacgaatt
     3061 gctatcgctc gagctctact caaaccaagc aatcttatct tggcagatga gccaacggga
     3121 tcattagatc aggaaaatag ggacttaatc ctacaatttc tcatagaaat gaataatcgt
     3181 ggtaagacag tgattatcgt cacacacgat ccatatattg ccgataagtg tcagcggatt
     3241 gttcgtttat gatttagata gtttctatat ccttggcgaa tcaggcattc cactacaact
     3301 gcaaaaatca atgctctaga acgtatgcca tgcattactt gacccaagtt tacaataaat
     3361 aaacacaaca cttatctagt tctttcataa aacatagcca ggaggtacaa ccatgactgt
     3421 tgcattcatc atttttttaa tcataaaagt attagctctc attctattcc ttgcttgttt
     3481 atttctaggt tataaactag ttaaaacatt aaataaaaag caagtatctc ctgcaaaaaa
     3541 atggggaatc attggtctct atagcctatt tttgcttatc gtagcctatc ttgcaacatt
     3601 tgttgtcgta ctgggaatca atacttaggc ttcgaatttt gtgatatata tgtaaggaga
     3661 gacaataata gatatcaaaa cgaacaaaac cttattaagg caaattgaga ctatccttaa
     3721 cactcagctc ttatcggtcc aagagcttgc gatattaaaa catgcacaaa ctgcccttca
     3781 acatgacgaa taccttttaa aggtcatcgc ttacttaaaa caagaacttt cgcctcttgc
     3841 aataagcaac tcattgactc cagaagtttc agagttattt cttgaaataa acagacagca
     3901 tccagcaaca cgagctgccg cgatttggaa ttttcttgtt ccaaaaacta aataaacggg
     3961 aatggacata atcaacatag atatcttgat aatctactaa tctcagggat aaatccaacc
     4021 attttcttag gaggttttcg ccatagttta cttgaaatta atcattctct ggatttcgat
     4081 aacccttgca ttgtacgggg acaggttcat tcagaaagat ttcctaattt ggaaagggat
     4141 tttcctactt attgctctgg caagttcact ttcttttcta ttttaatccc acatagagta
     4201 ttttggaaag gaaaacagtt atgaaaccaa ccaatctttt tcataaaaca tcgaccgttt
     4261 tcactctgat tggcctccac ttagttgtct tcctacttag tggacttcgc tcttggatgt
     4321 tgcaccaacc cttcaaatgg tggattaccc tagggattag tgtcggtttt tcagttgctt
     4381 atctcttagt aacaaaaaat gactcctagt catcaggaaa ttgctgataa actacatcag
     4441 atgaatatga aaacattcca agaaaatcta tacaagactg aatgtgtcaa gttatctagc
     4501 ttaatcttga agtcttgtag aacaaatact taaaaaccag tcaatcacag aaatagattg
     4561 actggttttt ctgttcacat tctcatacca aatgacattc aaaatcagac tgtacgacac
     4621 agatagaact ctgtccctcc tattattcaa tggagtaggt tgtgattttt gaagtgtgct
     4681 attcaaacac aaactggttg tggtaaagtt cagcatagaa gccgttgagc ttgaggagct
     4741 ggctgtggtt gccttgctca atcacttttc cgtctttgag gacgatgttc aaccctcaaa
     4801 tctgacttta tttctccggt atgacccaaa acaaatagta agtgatgaag attgttaaca
     4861 gtcccagtcc tattttgacc aataaaatag gagccagcca gatagaaatg gccatgagta
     4921 agtaaatttg ccagatgatt tgcttttttc gtttgcggga aatggatttg gtctcaacgt
     4981 aatcagctac gtaaaattga taaatttttg tattgcgtag ccatttttca aacctttttg
     5041 aacttctcgc aaagcaaaaa ccagctaaaa gtaaaagagg ggttgtcgga actatcggta
     5101 aaacaatccc aatgacacca accaccaaac taacaaaacc aaatactaga tagaatattc
     5161 tctgcatact aatattatac caaaaaactc gtgaattttt gaacaatttt atcacatttt
     5221 cagaaagcgc ttgcatttta ttggataaaa gggtatgata aaggtaccaa atttaggaaa
     5281 gagagaagac gctatgaaac ttctaggact ggtcggaaca aactccgctc gctcaaccaa
     5341 ccgcaaactc ttgcagtaca ttgaacagca cttcgctgat aaggcagata tcgaactggt
     5401 tgaaatcaag gaactcccaa tcttcaataa accagccaat cgagaattac cagaaattgt
     5461 caaagaattg gtcgcaaaaa ttgaggctgc tgacggagtc atcatcggaa ctcccgaata
     5521 tgaccactct attccagctg tccttatgaa tgcccttgct tgggtatcat acggtgtcta
     5581 tccactatta aacaaaccag ttatgattac tggtgcctct tatggaacac ttggttcctc
     5641 tcgtgcccaa ctacagctac gtcaaattct caacgctcct gaactcaagg ccactgtctt
     5701 gccagatgaa tttttattgt cacattcctt gcaagccttt gatgccaatg gggagctcat
     5761 tgatttagaa accagtcaaa aactggatgc catctttgat gacttccgcc tctttgtcac
     5821 catgactagc aagctgtcta acgcaaaaaa attactccaa aaagaagctg aaaacttcga
     5881 ttgggaaaac ctgtaagaga ggaatatact atgaaatttg tcggaattgt tggatcaaat
     5941 gcagatcaat cttataaccg catgctattg caattcattc aacgccactt taaagtgaag
     6001 tttgaactag aacttttgga aatcaaagat gttccaatgt tcaatcagga cgaagaccaa
     6061 tctgattgtt ttgcaattca atacctttac aacaaaatca cccgtgctga cggggtcatt
     6121 attgctaccc ctgagcacaa tcataccatt acccctgccc ttaagagtac tctggaatgg
     6181 ctcagcttta atttgcaccc attcgaaaat aaaccagtta tgattatcgg tgcatcttac
     6241 tacgatcaag gaacttctcg tacccaagtt cacctacgga aaattttaga tgcaccaggt
     6301 gtcaatgctt acacactacc aggtaatgaa ttcctccttg gtaaggccaa agaagctttt
     6361 gacgataagg gcaatatcat caatgaaggc accgtgaaat tcttagaaag ctgtctagac
     6421 aactttatca agtatgtcgg tgttgtatcc aaattgaaaa aaccaaaacc aatcgcacca
     6481 gaagatttag actgtaccaa accaatcgca acgaccatca ctggtgtgga tcccgatgat
     6541 ccagattggc tagaaaaggc ttctaaattg gttaacgcag ttgagggaga cacctatgtt
     6601 aaactggaca atggtctgtt gaccgttaac caactcaata tgttcctcaa tgcgatgcca
     6661 tttgaattga cctatgcaga tgataataac cagttcctct actacaacaa tagtcaccaa
     6721 gaacctgata ccatgttggg taaacgggta cttgctcaag taggaaatcg cctgtcaact
     6781 gtccacggta cgctacctcc tgcccgcacg aagaatgttg aatgggttgt cggtaccttg
     6841 cgtaatggta accaggaata tgttcgtacc attgtacccg gtacccctgc tgaaattatc
     6901 aacacccaca actaccaagc tatgtactat cctgacggat cctacgctgg tatcaatgaa
     6961 attatcttta atttcaaacc atggctagat tggtatcttg aagctactgg tcaacgcttg
     7021 gttggtggtt cgggaactgc cgcagctcct gcacatgttg acgcaacctc tggtgcatca
     7081 gatgctggta gtgcccctgc tacttccaca ccgacagatg caaattctgg tgcatccgtt
     7141 cactaatcgt tcacatatag aggctgggca gaaagcccag cacctcttct cagagttcgt
     7201 gtcaacatct cagcgcagtg gttgattggc tttaacagtc tgggagactg ttaaaggtag
     7261 gagataagat ttgcaaagca aatctcagca gttcgttttt tacactccaa atctggccta
     7321 atcaactgtg cggaggtggg aagacgaact ctttttagac tggtcgagtt ctttcccact
     7381 cccatatttt tcaaacaaaa cattgataat gatttcacaa ggagttccta tgtcaccatt
     7441 tcaagaaaaa atttcagctg cagtttcaaa aaaagaagcc ctttttgacg aaagtctagg
     7501 ccgctatgcc ttgcgttcta tgtatgcagg agcctacctg actatgtcaa cagctgtcgg
     7561 tattatcgga gcagacgtta ttgccagtca atttccaagt ttgtcgcgct ttgtctttac
     7621 cttcatcttt gctattgggc tggtttttgt tcttattttt ggtggtgaat tagccacctc
     7681 taacatgatg tatctcacaa ctggtgtcta ctataagcaa attacctgga aaaaagtgac
     7741 acagatgctg ctatactgta ctttctttaa ctttgtaggc gctactattc tagcctggct
     7801 cttcaattag tccttctcct acctcaatct ctctgataag agctttgtcg tcaatgccgt
     7861 taccatcaaa cttggaaaat ctgactggag caattttatt gaagggatca cggctaatat
     7921 gtttgtaaat atggcattcc ttggcttcat gctcatcaaa gaacagtctg ctaagttctt
     7981 tgtcatcatt tcctccattt ttatgttcgt cttcctcatc aatgagcatc tggtcgccaa
     8041 tttcgcatct ttcatgctcc tagcttttaa cgctactcga agccatgtcg aagccttcaa
     8101 tatgagcaat atccttcgtc aatggattgt ggtctttttg ggaaattggc tcggagccgg
     8161 tctctttatc ggtatcgcct acgcttggct caatcaaacc aaaaccaacc atattgagca
     8221 gtaagcctac cacaaaaaca gccaagataa accacgtcct aaacgtttaa ggattgtggt
     8281 ctgcttatct tggctgttta tttatgtgac ttttagtccg tcacgcctcg taagtaaggt
     8341 gcgtgacaaa taaaccaccc gctatgcggg tgcgcgacga aggttatacc aaaaaaaact
     8401 ccaaacg
//
LOCUS       DATUCG010000108         8325 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.110, whole genome shotgun sequence.
ACCESSION   DATUCG010000108 DATUCG010000000
VERSION     DATUCG010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8325
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.110"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            101..1051
                     /locus_tag="VCA20_001893"
     CDS             101..1051
                     /locus_tag="VCA20_001893"
                     /inference="COORDINATES: protein motif:HMM:TIGR01167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEP1835654.1"
                     /translation="MSTKKTYLLRKTSIGLVSLAALAVVGTTVSANEIGVATAHGTGA
                     YADPIPTFNINTGELTAKGSSVLNSDYKPALDPSTVDITKHGEGVTAELPEVALGFDN
                     GEPYVYAHGTGVTASVELPALDPSTVDITKHGEGLTAPTLNEVAVALDENGLYFTNTP
                     VEAPTAEALPEVQVGGKDGEFYLYAKGEGATVELPTIDPATLGAPIETAKGESVTAEE
                     LPTVEVAKDEAGEYLFSKGASVTAAELPVIDPATLTVAPAQAPVEAPKAEVAAAVPTA
                     KAATSSAKLPETGEASTAVYFATALGLLGAAAVLAKKQEN"
     gene            complement(1497..2154)
                     /locus_tag="VCA20_001894"
                     /pseudo
     CDS             complement(1497..2154)
                     /locus_tag="VCA20_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2262..4754)
                     /locus_tag="VCA20_001895"
     CDS             complement(2262..4754)
                     /locus_tag="VCA20_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEP1835655.1"
                     /translation="MNEVYFTGTIDRIIFENPSNFYKILLLEIEETDADYDDYEIIVT
                     GTIADVIEGEDYRFYGNLVTHPKYGQQLQISRYERSKPTSAGLVKYFSSEHFKGIGRK
                     TAEKIVELYGEDTIDKILAEPEKLTQITGLSSKNMQAFVEKLRLNYGTELILAKLAEY
                     GIPNKLAFQIQDQYKEKTLQIIEENPYQLVEDVQGLGFTIADRIAENLGIASDSPQRF
                     RAGMLFSLIHRSMETGDTYVEARDLLEATLELLEKSRHTELDPAAVAKELTGLIADDK
                     VQQEGTKIFDNSLYFAEHGIHKNLTRLMGKNGFKPFPRADVEAAIAELETMSSLTYDD
                     IQKEAIVQAITNPLFILTGGPGTGKTTVINGIIAVYAILHKIDLTRNREECPVLLAAP
                     TGRAARRMNELTGLPSATIHRHLGLVEGQEESYRDDYLDADFIIVDEFSMVDTWLANQ
                     LFQNISSQTQVLIVGDAEQLPSVSPGQVLADLLKIDKLPSITLERIYRQSDDSTIVTL
                     ASQIRQGALPSDFREKKADRSYFEAQNEQIPALIERIVGAAIKSGIPANEVQILAPMY
                     RGAAGIDQLNTMTQALLNPLKDGELEFLHNEQAFRQGDRVIHLVNDAEANVFNGDLGY
                     ITDLLPAKYTDSKQDEITINFDGSEVTYPRNEWYKIALAYAMSIHKSQGSEFQVVILP
                     ITRTSHRMLQRNLVYTAITRSKSKLILLGEISAFDYAVKNAGTVRKTYLVPRFQGEMA
                     EQDSKESLIKKDESKTAATTQTQPPTQPDRKEQVKVVKENNQQLSLLDQDQPEKTSPQ
                     PREYILTVDNLLTIDPMIGIQEADIEEFFKNK"
     gene            complement(4818..5447)
                     /gene="lepB"
                     /locus_tag="VCA20_001896"
     CDS             complement(4818..5447)
                     /gene="lepB"
                     /locus_tag="VCA20_001896"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEP1835656.1"
                     /translation="MGAIQKTKRSSLVAFLAEWGIFLLFMAAFFASRYFIWDPVSVDG
                     HSMDPTLQHQEKLIMLKTTSIDRFDIVVASETDSDGKEKLIVKRVIGMPGDTIRYEND
                     VLYVNDQKVDEPYLDEYLAAFQKDKLQEVYSYNKQFQAVAQSAEAFTQDANGYVDFTV
                     TVPEGQYYLMGDDRLVSLDSRSVGTFSRENIKGEVVFRMWPLNRIGTVD"
     gene            complement(5457..6347)
                     /locus_tag="VCA20_001897"
     CDS             complement(5457..6347)
                     /locus_tag="VCA20_001897"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEP1835657.1"
                     /translation="MNTVVLKASAQQKQAMMAHYDRYRQTSKNPYIEAFFKLTGASLS
                     IYTSGKVVFQGEMAEQEAGLWGYEPESSGQTTIPGQNLPMIGTDEVGNGSYFGGLAVV
                     ASFVRPEDHAFLKSLGVDDSKKMTDQKICQIAPLLKEKIPHQALLLSPKKYNEVIEQG
                     YNAVSVKVALHNQAIFLLLQKGVQPEKIVIDAFTSSQNYQKYLKKEANQFANPVTLEE
                     KAEGRYLAVAVSSIIARAMFLENLVQLGQLVGMNLPSGAGSKSDQVAASILKDYGMAG
                     LNQTAKLHFANTQKAQKLLK"
     gene            6432..7415
                     /locus_tag="VCA20_001898"
     CDS             6432..7415
                     /locus_tag="VCA20_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEP1835658.1"
                     /translation="MEAQMKYKWATLGTGVIANELVQALQAMGGNLYSVANRTYDKGV
                     EFAQKYGIEKVYREIDEVFTDPEVDIIYISTPHNTHINYLRKALKAEKHVLCEKSITL
                     NSEELAEAIQLAEENQVVLAEAMTIFHMPIYRQLSEVIASGKLGDLKMIQMNFGSYKE
                     YDMTNRFFNKNLAGGALLDIGVYALSFVRWFMAEKPNQVLSQVKLAPTGVDEQIGILL
                     SNDAGEMATIALTLHAKQPKRGTIAYDKGYIELYEYPRGQKAVITYTEDGSQEVIEAG
                     ETARVLSYEVADMEKAVAGIENTMHLAFTQDVMEIMTQLRKEWGLVYPEEM"
     gene            7764..>8325
                     /locus_tag="VCA20_001899"
     CDS             7764..>8325
                     /locus_tag="VCA20_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEP1835659.1"
                     /translation="MKRKRTNKPQHMRRKRKTPIMKNNKKMLYTSSLALSLFSTGMIS
                     TNVLAIEWAPRTVSEISPEIVQEEGKMTYTVQYGDTLSAIALAMNIDMDLLAKINQIA
                     DVNLIFPDTVLTTTVDQNNQVTQVEIEAPVQGNTNETVQATVDLTTNQVTVEDTVVPL
                     DQISSVTDSAPVEEVVEQPVVEAPVEE"
BASE COUNT         2242 a         1673 c         2005 g         2405 t
ORIGIN      
        1 catttatagg gtgatggtta ggataagagc cacggataat ggtgaaacat tatctcagct
       61 ttatttcaaa tgtagattat atataaggga aggtgacatt atgtcaacaa agaaaacata
      121 tcttttgaga aaaacctcta ttggtttggt atcattggca gctcttgcgg ttgtaggaac
      181 aactgtatca gctaatgaga ttggtgtagc aactgcacat ggtacaggtg cttatgcaga
      241 tccaatccca actttcaata tcaatacagg tgaattgaca gcaaaaggta gcagcgtatt
      301 gaactctgac tacaaaccag ccttggatcc aagtacggtg gacatcacaa aacatggcga
      361 gggtgtgact gcggaacttc cagaagtagc acttggtttt gacaatggtg agccatatgt
      421 ttatgcgcac ggtacaggtg tgactgcatc agtagaattg ccagccttgg acccaagtac
      481 tgtagatatc acaaaacatg gtgagggcct taccgcacca acattaaatg aagtagcggt
      541 tgccttggat gaaaatggtt tgtattttac aaacacacca gttgaggctc caacagcaga
      601 agcgcttcca gaagtgcaag taggtggtaa agatggagaa ttctatcttt atgctaaggg
      661 tgaaggtgcg acagtagagc ttccaacaat tgacccagca acacttggtg ctcctattga
      721 aacagctaag ggcgagagtg taacagctga agaattacca actgtagaag ttgctaaaga
      781 tgaagcaggt gagtatctct tctctaaagg tgctagtgta acagcagcag agctgccagt
      841 aattgaccca gcaacactta cagttgcacc agcacaagcg ccagttgaag ctcctaaagc
      901 agaagtggct gcagcagtac caacagctaa agcagcaaca tcatcagcaa aacttccaga
      961 aacaggagaa gcatctacag cagtttattt tgctacggct ctaggacttc ttggtgcagc
     1021 agcagtgctt gccaaaaaac aagaaaacta atcaatcatc agtaactaat cacacttaag
     1081 gagggttccc tccttatttt tgtgcagata gaagggtttg ggaaggatga tgacactctt
     1141 tgttccaagt ctaccttagt tggtaatact ttttgaaatt aaacatatct gtagtcaact
     1201 taccttgctg aactcaggtc gaccctaggc tctcattgat tttcattgag cataacatag
     1261 ccgatttcct cacaagtgcg ctactctttg aaagaatagg tagctattgt tgacttgctc
     1321 tttatttcta tggagagcga aatagagaac aatagaaaaa gacaaacact gtaaataaga
     1381 tgtttgtctt cttctcggaa agggatgcac gaatattcct ttaagtttgt tagagaatag
     1441 gggaataaac cttgtggtta ctcgaccttg tccaaggttg catataccta aggttcttat
     1501 actagatcgt acacggattc tacagatggg ttaaaaggat ttccccacat aatgaccttg
     1561 tattgtccgt cctcgaggat aaaacctggt gcaatatgtc cgccgaggcc agttactcct
     1621 ggaagaaggg cagaaccttc gccatcaact ccacgaatca ggtagtggtt tagagcatat
     1681 tcaaccagat ctgtaactcg agctgtgtag gtctggatga gctgttctcc ttcctcgtct
     1741 tcactaagtc gaaaatcttc ataagtagta aagttaccgt ctgcgctgat agttacttca
     1801 acattgcgtg cttgctggct gcttccttgc catttgccga ctagattatc tggaatagta
     1861 gtagctggca aagttagtag ttgagtgtga gttgtttcgg tacttaggac actttcttgg
     1921 gcttgagagt tctgagtttc ctggttttgt tgttcttgtt gactattctc tgctgatgaa
     1981 ctggcttcag ttgtattttc ttgggaacta gcaggctggg aggcgctagt ctgattggta
     2041 cttgcttggg tcgttttact agtggctgct tcggtctggc ttttttgctg aacaagctgt
     2101 caatagacag gttacaggga gcaacaatac tgagtagttg aatatctttt tcatttgttc
     2161 tcacctctac ttataaacag aggtaagaac aaataaaaac aagtttgtca acataaaaat
     2221 gtaatatttg caacattttt gtaataaaag aaatagtttt gttacttatt cttgaaaaat
     2281 tcctctatat ctgcctcttg gatgccaatc atggggtcga tggttagcag gttgtccaca
     2341 gtcagaatat attctctggg ttggggactt gttttttctg gctggtcttg atcaagaagg
     2401 gagagttgtt ggttgttttc tttgactact ttaacctgtt ctttcctgtc tggctgagta
     2461 gggggctggg tttgtgtggt tgcagctgtt ttactttcgt cttttttaat caggctttcc
     2521 ttgctatcct gctctgccat ttccccttgg aaacgtggga ccagataggt ttttcggacg
     2581 gtgccggcgt ttttgacggc gtagtcgaag gcggagattt cgcccaggag gatgagctta
     2641 ctcttggagc gggtgatggc ggtgtagacc agattgcgct ggagcatgcg gtggctggtg
     2701 cgggtgatgg gaaggataac gacctggaac tcactacctt gggacttgtg gatggacatg
     2761 gcataggcta gggcaatctt gtaccactca ttgcggggat aggtgacctc gctaccatcg
     2821 aagttgatgg taatctcatc ctgcttggag tctgtgtact tggcaggtag aaggtcggta
     2881 atgtagccca aatcaccatt gaagacattg gcctcggcat cgttgaccaa atggatgacc
     2941 cggtcgcctt ggcggaaggc ctgctcattg tggaggaact caagttctcc gtcttttaga
     3001 ggattgagca gggcctgggt catggtgttg agttggtcaa tgcctgcggc cccacggtac
     3061 atgggagcga ggatttgtac ttcgtttgca ggaatgcccg acttgatggc tgcaccgaca
     3121 atgcgctcaa tcagggctgg aatttgttcg ttttgcgctt caaaatagga gcggtcagcc
     3181 tttttctcac gaaaatcgct cggcagggcc ccttggcgaa tctggctggc tagggtaaca
     3241 atggtcgaat catcggattg acgatagata cgttctagag tgatgctggg tagcttgtca
     3301 attttcaaga gatcagctag aacctggccg ggactgacag aaggtaattg ctccgcatcg
     3361 ccgacaatca gaacttgggt ctgggaggag atgttctgga agagttggtt tgccaaccaa
     3421 gtatctacca tagaaaactc atccacgatg ataaagtctg catccaagta atcatcacgg
     3481 taggattctt cctgtccttc aacaagcccc agatggcggt ggatggtggc ggaaggcaga
     3541 cctgtcaatt cattcatccg tctggctgct cgtccagttg gagcggctag gaggactggg
     3601 cattcctctc ggttgcgagt caggtcaatc ttatgcaaga tggcatagac tgcgataata
     3661 ccgttgataa ccgttgtttt tccagtccct ggtccgcctg tcagaatgaa aagcggattg
     3721 gtaatggctt ggacgatggc ttctttttga atgtcatcgt aggtcaggga agacatagtt
     3781 tccagctctg cgatggcagc ctcaacgtct gcgcgtggga agggtttgaa accatttttt
     3841 cccatcaggc gggtcaggtt tttatggata ccatgttcgg caaagtagag gctgttgtca
     3901 aaaatctttg tgccttcttg ctgaaccttg tcgtctgcaa tcagtcccgt caattctttg
     3961 gcaacagcag cggggtccag ttctgtatgg cgggattttt ccagcagttc aagggttgct
     4021 tccagcaagt ctctggcctc cacataggta tcgcctgttt ccatggaacg atgaatgagg
     4081 ctaaagagca tgccagcccg aaaacgttgg ggtgaatcac tggcaattcc caggttttcc
     4141 gcaatcctat cggcaatggt aaagccgagc ccctggacat cttcgaccag ttgataggga
     4201 ttttcttcaa taatctgaag tgttttttcc ttatactggt cttggatttg aaaggccaat
     4261 ttattgggaa tgccatactc ggctagtttt gccaagatca actcggttcc ataattgagg
     4321 cggagtttct ctacaaatgc ctgcatgttc ttgctggata ggcctgtgat ttgggttagt
     4381 ttttcaggtt cagctaaaat cttatcaatg gtatcttcgc catagagctc aacgattttt
     4441 tcagctgttt tacgtccaat tcccttgaaa tgctcgctgg agaaatactt gactagacca
     4501 gctgaggtag gcttgctgcg ttcgtaacgc gaaatttgca gctgctgacc atacttggga
     4561 tgggtgacca gattgccata gaaacgatag tcttctcctt caatgacatc agcaatcgtt
     4621 ccggtcacaa taatttcgta gtcatcatag tctgcgtctg tctcctcgat ttcaaggaga
     4681 aggattttat aaaaattact aggattttca aaaataatcc ggtcaatagt accggtaaaa
     4741 tacacttcgt tcatagagtt tatttccaat aataatgaaa aagaagtttg gaacggattg
     4801 ctccaaactt gtttgtatta atcaacagtc ccaatacggt tgagaggcca catgcgaaag
     4861 acaacttctc ctttgatgtt ttcacgggaa aatgttccta cactacgact atctagtgag
     4921 acaaggcggt catctcccat caggtagtat tgtccttctg ggactgtcac ggtaaagtct
     4981 acgtaaccat tggcatcttg tgtgaaagct tcagcagatt gggccacagc ttggaattgt
     5041 ttgttgtaag aatagacttc ttgaagttta tctttctgga atgcagctag atattcatct
     5101 aagtaaggct cgtccacttt ttggtcgtta acatagagga catcgttttc gtagcgaatg
     5161 gtgtcacctg gcataccaat cacacgtttg acaatcagtt tttccttgcc gtcgctgtct
     5221 gtctcactgg ctacaacaat atcaaagcgg tcgatagagg tcgtttttag catgatgagt
     5281 ttttcttggt gttgaagcgt tgggtccatg gaatggccat cgacagatac aggatcccag
     5341 ataaagtaac gactggcaaa aaaagctgcc ataaatagta aaaagatgcc ccattcagct
     5401 aagaaggcga ctagtgatga gcgttttgtt ttttggattg ctcccatatt ttttcctcat
     5461 ttcaataatt tttgtgcttt ttgcgtattg gcaaagtgaa gttttgcagt ttggttgaga
     5521 ccagccatgc catagtcttt taagatgctt gctgcgactt ggtcggattt actgcctgca
     5581 ccagacggca gattcatccc aactagttga ccaagctgga caagattttc taaaaacata
     5641 gctctagcaa taatagaaga tactgccaca gccagatacc tgccctcagc cttttcttct
     5701 agagtgacgg ggttggcaaa ttggttggct tcctttttca agtatttttg ataattttga
     5761 ctggaagtga aggcgtcaat gacaatcttc tcaggctgga cacccttttg caagagtagg
     5821 aaaatagcct gattgtgcag ggcgaccttg acagacaccg cattgtagcc ttgctcgatg
     5881 acttcgttgt attttttcgg tgacaatagc agagcttggt gggggatttt ctcttttaag
     5941 agaggagcga tttggcagat tttttggtct gtcattttct tggaatcatc cacaccaagc
     6001 gacttgagaa aggcatggtc ttctgggcgg acaaaactag caacgactgc taaacctcca
     6061 aaataagacc cgtttccaac ctcatcggta ccaatcatgg gcaggttttg accaggtatg
     6121 gtcgtctgtc cagagctttc tggctcatag ccccagagcc ctgcttcctg ctctgccatt
     6181 tccccctgaa aaacgacttt tccagaagtg tagatggaca ggctggcacc ggtcagtttg
     6241 aaaaatgctt ctatgtaagg atttttgctg gtctgccgat agcggtcata gtgagccatc
     6301 atggcttgtt tttgctgggc agatgccttt agtacaacgg tattcatgtt cttattgtaa
     6361 cacaaatgct agttagagtg tgcagaaaag ggtggaattt actataatgg tagaagaaag
     6421 tgaggtacac tatggaagcg caaatgaagt ataaatgggc gactctggga acaggtgtca
     6481 ttgccaacga attggttcag gccttgcagg ctatgggtgg aaatctttat tcggtagcca
     6541 accgtaccta tgacaagggt gtggaatttg cgcaaaaata tggcatcgag aaagtttatc
     6601 gagaaatcga tgaggtgttt acggatcctg aagtagacat tatctacatt tcaacgcctc
     6661 acaataccca tatcaattat ttgagaaaag ctttgaaggc tgagaaacat gtcctctgtg
     6721 aaaagtctat tacgcttaat tcagaagaat tggcagaagc tattcaatta gctgaggaaa
     6781 atcaggtcgt cttggcggag gccatgacca ttttccacat gccaatctat cgtcagttaa
     6841 gtgaggtcat tgccagtggc aagcttggag atttgaagat gattcagatg aactttggca
     6901 gctacaagga atacgacatg accaaccgct ttttcaataa gaacttggcc ggcggtgccc
     6961 tcttggatat cggtgtctac gctctgtcct ttgtccgttg gtttatggct gaaaagccaa
     7021 atcaagtttt atctcaggtt aaattagctc cgacaggtgt ggacgagcag atagggattt
     7081 tgctcagcaa tgatgcaggg gaaatggcga ccatcgccct gaccctccat gccaaacagc
     7141 caaaacgggg tactattgcc tacgataaag gctacatcga gctttacgag tacccacgtg
     7201 gtcagaaagc agtcattacc tatactgaag acggtagcca agaggtaatc gaagcgggcg
     7261 agacagccag ggtcctttct tatgaagtgg cggatatgga aaaagctgtc gcaggcatcg
     7321 aaaacaccat gcatctagca tttacccaag atgttatgga gattatgacc caactccgta
     7381 aagaatgggg cttggtttat cctgaagaaa tgtaggatct tgactaatcc gaaaatagaa
     7441 atgagagtga gttagtagcc aattgttgga ttactgcctt actcttattt tttacttata
     7501 gtcttcgaaa atccagacgt tgttgacttg atttggtgaa cgttagttct atctgcatct
     7561 gcgtctccga gtctgttttt gattttcatt gagtattatc taataatatg acagtcagat
     7621 gacatttata ttgattgtgt gacagaaaag tcctaagatt acatagtgga gacagtttat
     7681 ttaaaagaac ggttgaaggc ttgtttttca gatttaattt aggtaagata gaaccatctc
     7741 aaaaataaag aaggaataag attatgaaac gtaagagaac aaataaacca caacatatgc
     7801 gtcgcaagag aaaaacacct atcatgaaaa acaataagaa gatgttatac acatcttcat
     7861 tggctctttc cctctttagt acagggatga tttcgacaaa tgttttagct atcgaatggg
     7921 ctccacgtac tgtttctgaa attagcccag aaattgtaca agaagaagga aagatgacct
     7981 atactgttca gtatggagat accttatctg ccatcgcctt agctatgaat attgatatgg
     8041 acttgctggc gaaaataaat caaattgcag atgtcaactt gattttccct gatacggtac
     8101 tgacgacgac tgttgaccaa aacaatcaag tgactcaggt tgagattgaa gctcctgttc
     8161 agggaaacac aaatgagacc gttcaggcaa ctgttgacct aacaaccaat caagtaacgg
     8221 ttgaggatac ggttgttccc ttggatcaaa tttcatcagt taccgactca gcgcccgtag
     8281 aggaagttgt agagcagcct gtagtagaag cacccgtaga ggaag
//
LOCUS       DATUCG010000109         8275 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.111, whole genome shotgun sequence.
ACCESSION   DATUCG010000109 DATUCG010000000
VERSION     DATUCG010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8275
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.111"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            153..1346
                     /locus_tag="VCA20_001900"
     CDS             153..1346
                     /locus_tag="VCA20_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEP1835660.1"
                     /translation="MNQNVTNLKLAERGALLSIGAYIVLSGIKLVAGQLFHSDALRAD
                     AFNNISDIIGNIAVLVGLKMAQKPADTDHKFGHWKMEDLASLITSFIMFVVGFQVLYD
                     TLQKLISNSSIEVDIMGAIVGIFSALVMLAVYLYNNRLAKKVRSKALEAAAKDNLSDA
                     VTSIGTSIAIFAAAFNFPIVDKIAAIIITFFILKTAYDIFMESFFTLSDGFDENLLKK
                     YEEDILKLPKIVSVKSQRGRTYGANIYLDVVLEMNPDLSVYESHEVTEQVEQLLTLKH
                     GVFDVDIHVEPSEIPHDEMYEHVYDKLFRFETEIQAHANGYEELIDNQYLLIDAKGHY
                     RNKTQILADHPIQTTYLSNYQMTSISHKSKLVTFEIGEYVHTSLWRRHENWTVIFHQI
                     SKKQA"
     gene            complement(1421..2251)
                     /locus_tag="VCA20_001901"
     CDS             complement(1421..2251)
                     /locus_tag="VCA20_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEP1835661.1"
                     /translation="MKKMMLGALALVTSLTLAACGSNDSATKSDNWTAYESEKSVTIG
                     FDKTFVPMGFEQTDGSYTGFDIDLANAVFEKYGITVKWQPIDWDLKETELNNGNIDLI
                     WNGYSITDERKEKVLFTNPYMDNQQVLVTKKSSNISQVSDMKDKILGAQAGSSGYSVF
                     ESQPAILKDIVQNNDASQYATFNEALIDLKNDRIDGLLIDRVYANYYLQQERIIADYN
                     IIDAGFENEAFAVGARKSDTTLVENINKAFAELYKEGKFQEISQKWFGEDVATDTVKK
                     "
     gene            complement(2278..2910)
                     /locus_tag="VCA20_001902"
     CDS             complement(2278..2910)
                     /locus_tag="VCA20_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEP1835662.1"
                     /translation="MLELRNLSKRFEDKQIFSNYDLVIPEGKIVAIVGQSGGGKTTLL
                     RMLAGLETIDSGTLMYNGQELPLEELGKRHLLGFVFQDFQLFPHLSVLENLVLSPMKT
                     QNMSRSEAEDKALKLLDTLGLANHATAYPFSLSGGQKQRVALARAMMIDPEIIGYDEP
                     TSALDPELRKEVEKLILENRATGITQIVVTHDMQFAENIADEIIKIEPKH"
     gene            complement(2920..3561)
                     /locus_tag="VCA20_001903"
     CDS             complement(2920..3561)
                     /locus_tag="VCA20_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004228788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEP1835663.1"
                     /translation="MDYVLEVLPSLLNGAAVSLQVFFLVLILSLPLGAVFAFLMQIKF
                     KPLQWLLHFYVLIMRGTPLLLQLIFVYYVLPSVGITFDRMPAVILAFTLNYAAYFSEI
                     FRGGIEAIPKGQYEAAKVLKFTPVQTIRYIVLPQVVKIVLPSVFNEVMTLVKDTSLVY
                     ALGVSDLLLASRTAANRDASLAPMFIAGAIYLLMIGAVTLISKQVEKKFDYYR"
     gene            complement(3715..5526)
                     /gene="glmS"
                     /locus_tag="VCA20_001904"
     CDS             complement(3715..5526)
                     /gene="glmS"
                     /locus_tag="VCA20_001904"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEP1835664.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVTGGEQAHL
                     VKAVGRIAELSAKVGDKTEGTTGIGHTRWATHGKPTENNAHPHTSQTVGHILVHNGVI
                     ENYVEIKEEYLAGHDLKGQTDTEIAVHLIGQFAEEGLSTLEAFKKALKIIQGSYAFAL
                     IDATDADTIYVAKNKSPLLIGLGDGYNMVCSDAMAMIRETSEYMEIHDKELVIVKKDS
                     VEVMDYDGNEIERGSYTAELDLSDIGKGTYPYYMLKEIDEQPTVMRKLISAYTNEAGQ
                     VTVDADIIKAVQEADRIYILAAGTSYHAGFASKDFLEKLTDTPVELGISSEWGYNMPL
                     LSKKPLFVMISQSGETADSRQVLVKANEMGIPSLTVTNVPGSTLSREATYTMLLHAGP
                     EIAVASTKAYTAQIATLAILAKAVGDANGNAYAKEFDLVHELSIVAQSIEASLSEKDV
                     IAEKVEKLLSTTRNAFYIGRGSDYYVSMEASLKLKEISYIQCEGFAAGELKHGTISLI
                     EDGVPVLALISNHPHLASHTRGNIQEVVARGANVLTIVDEAVAKEEDDITVTTVHPFL
                     SAIAMVVPTQLIAYYATLQRGLDVDKPRNLAKSVTVE"
     gene            complement(5716..6258)
                     /gene="lepB"
                     /locus_tag="VCA20_001905"
     CDS             complement(5716..6258)
                     /gene="lepB"
                     /locus_tag="VCA20_001905"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEP1835665.1"
                     /translation="MVKRDLIKQISLLVLLILGIIGLRFWLLEPVTITPEMANSYLKE
                     NDFIMTVRNVRPIHGDFILYNHEGKEYVSRVIALENETVTYMDDVLYRNDIIVTENYL
                     KTPHSQESYTDDFTLETLTNGKYNIIPEGHYLVLNDVRTNQQDSRSFGLISSEAIVGR
                     LTFRISPLSEFGFIKTGLVQ"
     gene            complement(6301..6918)
                     /locus_tag="VCA20_001906"
     CDS             complement(6301..6918)
                     /locus_tag="VCA20_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HEP1835666.1"
                     /translation="MSDIMYPEVLTVGSGAVKVATVGDSLTYGYGLENREKDAYPCIL
                     AEKLGHHYQVSNYGLSGRSLQSTADFPYFKENNAQLSLESEADIVIIMIGSNDSRGPY
                     WNRERFIREYREMAEQYMDLPSQPDVYLVIPPYVPTSRFGLNNQIIEDELQKIIPAIG
                     NELGLPVINLYTVTEGHLEYYSDGLHLTPLGNQVIAEEIYQHLRH"
     gene            7003..8112
                     /locus_tag="VCA20_001907"
     CDS             7003..8112
                     /locus_tag="VCA20_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEP1835667.1"
                     /translation="MKKQVIIIGGGIVGSTAAFYLSQEEQVNLTLIDSGVGTATRAAA
                     GIICPWMAQKKNKDWYKLTSDGAVFYRQLVADLEKSGATEIPFKQTGTIGLKSKPELL
                     DKIQKIAEDRRVDTPTIGEITPLIGSEIKQYLPLLKPDYFGIHLEGGGRIDGGRLIDI
                     LQEQFLKNGGTRIYGQAKLLDNHTVEVDNQVLSADHIILATGAWLPHILEPLGYQVDV
                     RPQKGQLLELGTEFDTDNWPVCMPYGEIDILPFENGKIIIGATHEDDMGYDLELDPEK
                     IQAMHDKIAEFMPDLANYPVARTRIGTRAYTSTYSPFYGNIEDKPNVWVASGLGSTGL
                     TNGPIIGWQIAKEILNQETNFDRTPYSPNRYILKK"
BASE COUNT         2573 a         1759 c         1457 g         2486 t
ORIGIN      
        1 ttgtaaaaag gtagatacta agtttaaagc agttatttct ggggacaaaa ggagagaaga
       61 aatttttcat gcgtatggcg tgaaaaagca gcctaacccc ttgaaaataa ggggcctttt
      121 tgttaaaatg aaaacttatc aaaggagatg ctatgaacca aaacgtaacc aatctaaaac
      181 ttgctgaacg tggtgccttg ctatctatcg gtgcctatat tgtcttatca ggtatcaaac
      241 tcgttgcagg acaactcttt cattcagatg ccttacgagc agatgccttc aataatattt
      301 ccgatattat cggtaatatc gctgtacttg ttggactaaa aatggcacag aaaccagctg
      361 ataccgatca taaatttgga cactggaaaa tggaagactt ggcaagcttg attacatcct
      421 tcatcatgtt tgtcgttggt ttccaagtac tctatgatac cctccaaaaa ctaatcagca
      481 attcttctat cgaagtagac attatggggg ccattgtcgg aattttttct gcactagtta
      541 tgttagctgt ttatttatat aataaccgct tggcaaaaaa agtacgttcc aaggccttag
      601 aagcagctgc caaggataat ctatccgatg cagtcacttc cataggaact tctatcgcaa
      661 tctttgctgc ggctttcaac ttccctattg tcgataaaat tgcagccatc atcattacct
      721 tctttatcct aaaaacagcc tacgatattt ttatggaaag cttctttacc ctatctgatg
      781 gatttgatga aaacttattg aaaaagtacg aggaagatat tctcaaactc ccaaaaatcg
      841 tttctgtcaa atcacaacgt ggtcggactt atggagccaa tatctacctt gatgttgttt
      901 tagaaatgaa tccagactta tccgtttatg aaagtcatga agtgactgag caggttgagc
      961 aactcctaac actcaaacat ggagtttttg atgtggatat tcacgtagaa ccatctgaaa
     1021 ttccacacga tgagatgtat gagcatgtgt acgacaaact cttccgtttt gaaacagaga
     1081 tacaagcgca tgctaatgga tatgaagaat tgattgacaa ccagtatctc ttaattgatg
     1141 ccaaaggcca ctaccgaaat aaaactcaga tacttgccga ccaccccatc caaacgacgt
     1201 atttatcaaa ttaccaaatg acatctatta gccataaatc aaaattagtt acgtttgaaa
     1261 ttggcgaata tgttcatacg tcactttggc gccgtcatga aaattggact gttattttcc
     1321 accagatttc aaagaaacaa gcctagtcga atgactggct tttcctttac cttaaatttg
     1381 gtttttaaaa aaagagcact gggctctttt tcttacatta ttattttttt actgtgtcgg
     1441 ttgcaacatc ctcaccaaac catttttggg agatttcttg gaatttacct tctttatata
     1501 attcagcaaa cgccttatta atgttttcta cgagtgtcgt atctgattta cgagcaccaa
     1561 ctgcgaaggc ttcattttca aaacctgcat caatgatgtt gtaatctgcg ataattctct
     1621 cttgttgcaa gtaataattt gcatacacac ggtcaatcaa aaggccatca atacgatcgt
     1681 ttttcaagtc aatcaaggct tcattgaagg tcgcatactg acttgcatcg ttattttgaa
     1741 caatatcttt caaaatagct ggttgtgatt caaatactga atagccagat gaacctgctt
     1801 gcgcacctaa aatcttatcc ttcatatcag atacttggct aatattggaa gattttttag
     1861 ttacaagaac ttgttggtta tccatgtagg gatttgtgaa aagaactttt tccttacgct
     1921 catctgtaat agaataacca ttccaaatta aatcaatatt cccgttattc aattcagttt
     1981 ctttcaagtc ccagtcaatt ggttgccatt ttacagtaat tccatatttt tcaaagactg
     2041 cattagccaa gtcaatatcg aaacctgtgt aagaaccatc tgtctgttca aatcccattg
     2101 gaacaaaggt cttatcaaag ccaatcgtaa cggatttttc agactcgtag gctgtccaat
     2161 tatcagactt agtagccgaa tcattggagc cacaagcagc aagagtcaaa gaagttacaa
     2221 gagcaagagc tccaagcatc atttttttaa tattcatcat catatttgtt ccttttctta
     2281 gtgtttcggt tcaattttga tgatttcatc tgcgatattc tcagcaaact gcatatcatg
     2341 ggtgaccaca atttgcgtga ttccagtagc cctattttca agaatcagtt tctcaacttc
     2401 ttttcttaat tcagggtcta gggccgaagt tggttcatca tagccgataa tctcaggatc
     2461 aatcatcatc gcacgcgcca aggccacacg ttgtttctgt ccacccgata atgaaaatgg
     2521 gtaggcagtt gcgtggttag caagtccaag agtatccaag agtttaaggg ccttgtcttc
     2581 cgcctctgaa cgcgacatat tttgcgtttt cataggagag agaaccaagt tttccaatac
     2641 ggataggtgt gggaaaagct ggaagtcctg gaagacaaag cccagcaaat gtcgttttcc
     2701 caactcctct aatggcagtt cttgaccatt atacatcaaa gttcctgaat caatagtctc
     2761 taaaccagcc aacatccgta aaagagttgt cttaccaccg ccagattgac caacaatggc
     2821 aacaattttc ccttctggaa tgaccaaatc atagttggag aaaatctgtt tatcttcaaa
     2881 gcgttttgac aaattgcgta actctaacat gacttcctcc tatctataat aatcaaattt
     2941 cttttctact tgtttggaaa tcagtgttac cgcaccgatc atcaataggt agattgcacc
     3001 tgcgataaac ataggcgcca agctggcatc acgattggcc gcagtacgac tagccaacaa
     3061 caaatcgcta accccaagtg catagaccaa agaagtatcc tttaccaaag tcatgacttc
     3121 attgaaaaca cttggcagaa caattttcac aacctgaggt aagacaatgt agcgaatggt
     3181 ctgaacaggg gtaaatttca ataccttagc agcttcgtac tgaccttttg gaatagcttc
     3241 aataccacca cggaaaattt cagagaaata ggccgcatag ttgagcgtaa aggccagaat
     3301 cacagcaggc attcgatcaa atgtaatacc cacacttggt aagacgtaat aaacaaaaat
     3361 taattgtaaa agcaaggggg ttcctcgcat aattaataca tagaaatgca ataaccattg
     3421 taagggttta aatttaatct gcattaaaaa agcgaaaaca gctcctaatg gcaaggataa
     3481 tatcaatact agaaaaaata cttgcaagga gactgcagca ccatttagta gactgggcag
     3541 aacttccaaa acataatcca tagagctctc ctaaaagaat tattagattc cagtatatca
     3601 aaaaatatcc ttaatttcaa agattttttc agaattttta ataaatttaa caaaattttg
     3661 tatatttttt caacagtgca aaaagaaggc tggaaatcca cgccttcctt cttcttattc
     3721 aacagtaaca gacttagcca agttacgtgg tttatcaaca tccaaaccgc gttgcaatgt
     3781 cgcatagtaa gcgatcaact gtgtcggaac caccatagca atcgctgaca agaatggatg
     3841 aacagttgta acagtgatgt catcctcttc cttagctact gcttcatcta caattgtcaa
     3901 gacattggca ccacgcgcca ctacttcctg aatattgcct cgagtatggc ttgcaaggtg
     3961 tggatgattt gaaatcaagg ccaagacagg aacaccgtct tcaatcaatg aaatggtacc
     4021 gtgtttcaat tcacctgccg caaagccttc acattggatg tatgaaattt ctttcaattt
     4081 caagctggct tccatagaaa cgtagtagtc actaccacga ccgatataga atgcgttgcg
     4141 agtggttgat aagagttttt caactttttc agcaatcaca tctttttctg acaagctggc
     4201 ctcgatagat tgagctacga tggacaattc atgcaccaag tcaaattctt tcgcgtaggc
     4261 attgccattg gcatcaccga cagccttagc caagattgcc aaagtcgcaa tctgtgcagt
     4321 gtaggctttt gtagaagcta ccgcaatttc tggtcctgca tggagcaaca tggtgtaagt
     4381 tgcctcacgt gatagggtag aaccaggaac gttggttaca gtcaaacttg gaatgcccat
     4441 ttcattagct ttaaccaata cctgacggct atcagcagtt tcacctgact ggctaatcat
     4501 tacaaagagt ggctttttgc tcaaaagtgg catattgtag ccccactcag aagagatacc
     4561 caattctact ggtgtatctg tcaatttttc caagaagtct tttgaggcaa atcctgcgtg
     4621 ataggaagtt ccagcagcca agatatagat acggtcagct tcttgtactg ccttaataat
     4681 atccgcgtca actgttacct gaccagcttc atttgtatag gcgctaatca atttacgcat
     4741 aacagttggc tgctcatcaa tttccttgag catgtagtat ggataagttc ccttaccaat
     4801 atctgacaaa tcaagctcag ctgtatagct accacgttca atctcatttc catcatagtc
     4861 catgacttca acactatctt ttttcacgat aaccaattcc ttatcgtgaa tttccatgta
     4921 ttcacttgtc tcacgaatca tagccatggc atctgagcag accatgttat agccatcacc
     4981 aagaccaatc aaaagtggag atttattttt ggcaacgtag attgtatctg catccgtagc
     5041 atcaatcaag gcaaaggcgt atgaaccttg aataatcttc aatgcttttt tgaaggcttc
     5101 tagagttgac aaaccttctt ctgcaaattg accaatcaag tgtacagcaa tttctgtatc
     5161 tgtctgccct ttcaaatcat gacctgcaag atactcttcc ttaatttcta catagttttc
     5221 aatcacaccg ttgtgaacta ggatatgtcc aacagtttga gaagtgtgag ggtgagcgtt
     5281 gttctcagtt ggcttaccat gagtcgccca acgagtatga ccgatccctg ttgtcccttc
     5341 agtcttatcg ccaacttttg cagacaattc tgcaatgcga ccaacagctt tcacaagatg
     5401 agcttgctca ccacctgtca caaaaatacc tgctgaatca taaccacggt attctaattt
     5461 ttcaagtcct tgaatcaaaa tatcagttgc gtttgtattt cctacaacac caacgattcc
     5521 acacatacat atcacctacg atgtttgtta acatcggctt tcatttcttt aaaaattggt
     5581 ctagtctatt ttatatctaa aaagaccagt gaaatcagta tataacaagt ttttaacctt
     5641 gtcaacattt taattggtct agtctaattt caaaatacag ttccagtcat ctcaatacaa
     5701 taaaaaaagc tgattctact gaaccagccc tgtcttaata aaaccaaatt ctgaaagtgg
     5761 actaatccga aaagtcagtc taccaacaat agcctcacta gaaatcaaac caaaactacg
     5821 actatcctgt tgattcgttc gtacatcatt caacaccaaa tagtgcccct ctggaatgat
     5881 gttgtacttt ccatttgtca aggtttccaa tgtaaagtca tctgtataac tttcttgtga
     5941 atgaggcgtt tttaaatagt tctctgtaac aataatatca ttacgataga gaacatcgtc
     6001 catataggta accgtctcgt tttccaatgc aatcacacga ctcacatact cttttccctc
     6061 atggttatac aaaataaaat ctccatgaat cgggcggaca tttcgcactg tcatgataaa
     6121 gtcgttttcc ttcaaataac tatttgccat ctcaggggta atcgtgactg gctcaagtaa
     6181 ccaaaatcgt aggccaatga ttcccaaaat caataagacc aataaactga tttgttttat
     6241 taaatctctt tttaccataa tctctccatt tcactagtat tataccacaa tttagagcca
     6301 ttagtgtctg agatgctggt aaatctcttc agcaataact tgatttccta gcggtgtcaa
     6361 atgaagacca tcgctatagt attcaagatg tccctccgtc acagtgtaaa ggttaataac
     6421 tggtaaacca agttcgtttc cgatagctgg gataattttt tgtaattcat cttcaataat
     6481 ttgattattc aatccaaaac gactggttgg tacgtaaggc gggatgacta aataaacatc
     6541 cggctggctt ggtaaatcca tatactgctc tgccatttcc ctatactctc gaataaacct
     6601 ctctctattc caatatggtc ctcggctatc gttactacca atcatgatga tgacaatatc
     6661 tgcttcactc tcaagtgaaa gttgggcatt attctcttta aagtaaggaa aatctgcagt
     6721 tgactgaagt gaacgcccac tcaatccgta atttgaaact tgatagtgat gcccaagttt
     6781 ctcagctaga atacaaggat aggcatcttt ttcccgattc tccaagccat atccataagt
     6841 caaactatct ccgactgttg caacttttac ggccccactt ccaacggtta ggacctcagg
     6901 atacattata tctgacacac gaactcctca atttttctta ttttctattg taccatgttg
     6961 aaaaaaataa ataaacttcc gaatttgtga tataattaac ttatgaaaaa acaagttatt
     7021 attataggcg gaggaattgt tggctctact gcagcctttt atctctccca agaagaacaa
     7081 gtcaatctta ctctcattga ttcaggtgtc ggaacagcca cacgagctgc cgcaggtatc
     7141 atctgcccat ggatggctca gaaaaaaaat aaagactggt ataagctgac ttccgatggt
     7201 gctgttttct accgtcaatt ggtagctgat ttggaaaaat cgggtgctac tgaaattccc
     7261 ttcaaacaga ctggaactat cggcctaaaa agtaagcctg aactacttga taaaatccaa
     7321 aaaatagcgg aggatcgtcg cgtagatact ccgactattg gtgaaattac tccgcttata
     7381 ggctcggaaa ttaaacagta cctgccatta cttaaaccag actattttgg tatccattta
     7441 gaaggtggcg gtcgtattga tggtgggcgt ttgattgata ttcttcagga acaatttttg
     7501 aaaaatggtg gtacacgtat ctacggacaa gcaaaactgc tcgacaacca tactgtcgaa
     7561 gtagataatc aagtgcttag tgctgaccat ataatcttag ccacaggggc ctggttgcca
     7621 catattcttg aacctcttgg ttatcaagtg gatgttcgac cacaaaaagg acaattgtta
     7681 gaattaggta cagaattcga tacagataat tggccagttt gtatgcctta cggagagatt
     7741 gacatcctac catttgaaaa tggaaaaatc attattgggg caactcatga agatgacatg
     7801 ggctacgatt tagaactaga ccctgaaaaa atccaagcca tgcatgataa aatagctgaa
     7861 ttcatgcctg atttagcaaa ctatcctgta gccagaactc gcattggcac gcgcgcctat
     7921 acttcaactt attcaccatt ctacggaaat attgaggata agcctaatgt ctgggtagct
     7981 agtggtctag gctccaccgg tctgacaaac ggtccaatta tcggttggca aattgctaaa
     8041 gaaattctca atcaagaaac aaactttgat cgtacaccat attctccaaa tagatatatt
     8101 ctcaaaaaat aagatacgat aagtttagat tctactaacc ctttaaatgt agatactaag
     8161 actagttgtt atctaaccaa taaatacaat aagcaacggg gaacctcgtt gcttatttct
     8221 atctagaata ctaccattct agtctcaaac tattaaagga ttatagtcaa ctgaa
//
LOCUS       DATUCG010000110         8073 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.112, whole genome shotgun sequence.
ACCESSION   DATUCG010000110 DATUCG010000000
VERSION     DATUCG010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8073
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.112"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            11..1945
                     /locus_tag="VCA20_001908"
     CDS             11..1945
                     /locus_tag="VCA20_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012961885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEP1835668.1"
                     /translation="MSFFDYQRLDDIVNLLLDKKEAVHIDELSSYCNVTDRTIRSDIN
                     TINDYIKSHGAHIILIRKKGYVIQYLDKNSFDEFWSNQDTGTFLFTTSEARLTFLIRL
                     FLTTDDYINQDYLQSILFVSQNTLYNDLRTLKQYLSRYQLKIQNKSNLGYKVIGQEQD
                     FRSAIVDLIFKSNFTEYLTASTSVEKDICNNINYDKFNSIYSHIFSKEIFIDSDYFHR
                     NIFTSLLLAISRAKSGHTLSKFDQDIKLFQHFTSVVNEFVETIETAFNLKLSILERNY
                     INFCIAENSPRLIDDTIMNENQELGERILVLIHNTLKKYTDSNWIYDSTLENNLLEHI
                     KLFLKVQTIEGNRINPLLETVKTNFPYAFDLAINCTREIVRCFNVHFSEDEISYIALH
                     LANAIERSNVKELKQLSLAIICGTGHTFSSIIEAKIKRRFPEVFSTIEKFSYTNFSAN
                     DNKFLNFDLIVSTIPIRKNTVDIVFIDINDLDYSMTKISQKINELSVNAINAPEGLLS
                     PKHFHVLHKKSTKEETLKYLYEELLSQGYVKENFLAEIYERENISSTIISDSIAIPHP
                     LGEAVTKSTIFPIIAPKGITWDNKLVKFVFLFAIKAKEPEKMEFIYERLLDFIGSEKL
                     QAKLLKEPTFNSLLSIFEEQ"
     gene            complement(2415..4223)
                     /locus_tag="VCA20_001909"
     CDS             complement(2415..4223)
                     /locus_tag="VCA20_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEP1835669.1"
                     /translation="MKTNNVEIIKADSLVRRRGVNVERHLKRVAAYCRVSSDSEDQKN
                     SYESQVRHYKDYISQRSDWELADIYADEGISGTQVGKRQDFQRLINDCVNGEIDYIVT
                     KAIARFARNTLDTLKYVRMLKDMQIGVYFEEENIDTLTMDGELLLTILSSVAQQEVEN
                     TSAHVKKGLKMKMQRGELIGFQGCLGYDYDVETKQLSINKKEAKIVRYIFERYLEGIG
                     GKVIARELDELGYKSPRGLDHWNDTTVLGIIKNEKYKGDILMGKTFTVDPISKRRLSN
                     FGEEDKYYIKDNHEPIISKEDFEKAQEIRLRRAGNKKTVANVNGKRERYSKMYAFSSM
                     LECGFCGSILSRRSWHCRSDYRKVVWHCVTSIKKGKKFCKHSKGLEELAIEGAFMEAY
                     RQLYHSNENLMTDLLETIESELNDNSLNKELKRITNKLRTLLKKEENLVNLRLEGKIS
                     DTIYNEKYNEISSEKEFLAEEKVNIETTLKSEIDVKKRLTEFKHLLSSQKMLTEFDRA
                     VFESIVEKIIVGGVNSDGEIDPAMLTIIFKTGETQNKDGKQFKNKRKNAKLETDKLCP
                     QNSDEDKKMYSQGPDNTRRVCCSARNSLETFERKGY"
     gene            complement(4684..4908)
                     /locus_tag="VCA20_001910"
     CDS             complement(4684..4908)
                     /locus_tag="VCA20_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP1835670.1"
                     /translation="MYRRVRDLREDCDFTQEYLANYLSCSQSAYSKIEAGRRQLSIDY
                     LIKLSDLYQVSTDYLLGLTDCPNRIKRKLK"
     gene            complement(5327..6382)
                     /locus_tag="VCA20_001911"
     CDS             complement(5327..6382)
                     /locus_tag="VCA20_001911"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835671.1"
                     /translation="MKAKNRRRIGYSVIEQYNSLVFLKDFLEDKKLEGYLATLNKSID
                     EVHRLSISEQQKIINLFPVNIPQIGGDFPKGQNNQYVYLNNTKLNKFLFQNHFDYNVW
                     REENGLRTIKEIDCLAYRYTKFESNDIFENLSIENILFLIKNIDLKLFSYVKRTVENN
                     IIYADIDTLRCVHLPLSETSFLVFPNLKKEAFSEIYILHEISHVILNYYQTKYDIILS
                     LEAEEGLAHFIESAICNNLIKEELDEYKRLLTNLLIENSCKLNFQIELFKNFKKYRTK
                     SSKEELYRSIFSTFGISVTDSFTTIFQELPPFYSASYIVGQEEAIKNIGNIDFFELIK
                     ILKIKLSSRDSNTITFY"
     gene            complement(6366..7556)
                     /locus_tag="VCA20_001912"
     CDS             complement(6366..7556)
                     /locus_tag="VCA20_001912"
                     /inference="COORDINATES: protein motif:HMM:NF019310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEP1835672.1"
                     /translation="MKSIVTKYFIILSGVSIGTALFNPILYIFLLTQGFSHYQLGIYL
                     ALFFVFSFLTEVPCGAITDTIGVRNTLVLSFCLRALGLLLLLSNIFQLLLLSSFISAV
                     AESFQSGTVQSWVVNSLDEIGEKEKIPLLFSRSSIISSILTMLFGFVSSKVLFNYDIK
                     LPIIVSVLTFVFLCLFVLLLFSSSTDKKKIDFSNIFIDSFQTLKSTLAELVLIKELKL
                     ILVLMLLPAVLDVGPANQWQEVFHDSIFTDLSGYIWIFISLFGMLGGFISEKIQKITN
                     NRIFITTLLFINILLIIFIGVLQKSMLTLLLFLLYILTFTILSIRINITLHNEIVVNN
                     EHRTTIVSLYYAIESFIMSLFIFINGLLSDLIPILKVWLIFAIAIACILIYLGARFAN
                     ESKK"
     gene            complement(7537..7821)
                     /locus_tag="VCA20_001913"
     CDS             complement(7537..7821)
                     /locus_tag="VCA20_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HEP1835673.1"
                     /translation="MSDVNNFFSNGKLITIPKKKRSKEKVFCFLYEQIVNRGNYFTEK
                     EINSILKMYYDDYAILRRYLVDYGFLARDRYGLEYVTVNKDWSDYEKHCN"
     gene            complement(7814..>8073)
                     /locus_tag="VCA20_001914"
     CDS             complement(7814..>8073)
                     /locus_tag="VCA20_001914"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835674.1"
                     /translation="LKDFIVGKEDRRKSDKVTRIMESDLGFINRFPIIENYGDLDHMS
                     LIHMSNIRLVGNIPTLENEIYKYLALYYKKIIFNNEGDSRE"
BASE COUNT         2771 a         1464 c         1032 g         2806 t
ORIGIN      
        1 ggaggttcat atgtcttttt ttgattacca aagacttgat gatatcgtaa atctattatt
       61 ggataaaaaa gaggctgtcc atattgatga actcagttca tactgtaatg ttaccgatag
      121 aaccatccgt tctgatatta ataccatcaa tgactatatt aaaagtcatg gggcacacat
      181 cattttaatt agaaaaaaag ggtatgttat acaatattta gataaaaatt ctttcgatga
      241 attttggtct aaccaagata caggaacatt cctgtttacg acttctgaag cacgattaac
      301 atttcttatt cgcttattct tgaccacaga tgactatatt aaccaagact accttcagtc
      361 catactattt gtcagtcaaa acacactata taacgactta cgtacactaa agcaatattt
      421 atctagatac cagttgaaaa ttcaaaacaa aagtaaccta ggctataaag tgattggcca
      481 agaacaagat tttagaagcg caattgttga cctcattttt aaaagtaatt ttacagaata
      541 tctcactgct tcaacttctg ttgagaaaga tatttgtaac aatataaact acgataaatt
      601 taacagtata tactcgcaca tattttctaa agaaattttt attgattccg attactttca
      661 tagaaatatt tttaccagct tactcctagc tattagtaga gctaaatcag gtcatacatt
      721 atctaaattt gatcaggata ttaaactttt ccaacatttt acttcagttg tcaatgagtt
      781 cgttgaaaca attgagactg cttttaattt aaaattatca attttagaaa gaaattatat
      841 aaacttttgt attgctgaaa actctccaag gcttatcgat gacactataa tgaatgagaa
      901 tcaagaatta ggtgaacgaa ttctcgtact cattcacaat actttaaaaa aatatactga
      961 ttcaaattgg atttatgatt ctaccttaga aaataactta ctagaacata taaaactctt
     1021 tcttaaagtc caaactatcg aagggaacag gatcaatcct ttacttgaaa cagtaaaaac
     1081 caatttccca tatgcatttg atttagctat taactgtact agagaaattg ttagatgttt
     1141 taacgttcat ttctcagaag atgagatttc ttatattgct ttacatctag ccaatgctat
     1201 agaacgtagt aacgttaagg aactcaaaca attatcattg gcaattattt gtggaacagg
     1261 acatacattt agttcaatta ttgaagcaaa aataaaaaga agatttcctg aagtgttctc
     1321 tacgatagaa aaattttctt atactaattt ttcagctaat gataataaat tccttaattt
     1381 tgacctcatt gtatccacta ttcctattag gaaaaataca gttgatatcg tttttattga
     1441 tatcaacgat ttggattact cgatgactaa aattagtcaa aaaataaatg aattatcagt
     1501 taatgctata aatgctccag agggactgct ttcacctaaa catttccatg ttttacacaa
     1561 aaaatccaca aaagaagaga ctttaaaata tttgtatgaa gagctattgt ctcagggata
     1621 tgttaaagaa aattttttag ctgaaatata tgaaagagaa aacatttcct ctactatcat
     1681 ttctgactca attgcaattc ctcacccact tggtgaggct gtcacaaaaa gtactatttt
     1741 cccaatcatc gcccctaaag gaatcacatg ggacaataaa ctagttaaat ttgtttttct
     1801 atttgccatt aaagcaaaag aaccagagaa aatggaattt atctacgaaa gacttcttga
     1861 tttcataggt tcagagaagc ttcaagcaaa attgttaaaa gagcctacct ttaattcttt
     1921 attatccata ttcgaagaac aataaaaaaa tcattaagtt aaaaaacagc agttttttta
     1981 aaaactcaat taaacacttt aatgatttca atcagtacaa actattaccc cgtctcttta
     2041 agttaccctc aaaatagtag gtaaaaccaa caaatcgaat ttcaacccac aagaaaagcc
     2101 cttgaaacca agcagtccca agggctttta atcgttattt tttcccaaag agaaggttaa
     2161 caaattttta ttcaactaga atcaaaaaat tgtatcaaaa actccactca cacttcaatc
     2221 ggccattcaa gcgatattag aggtggtacg tttctattcc cacaaactcc ttgtcaatgg
     2281 aaacaaacac atacccatag ggtaaatgga aatagaaaca gataatttct agctatcact
     2341 tctactcact ccaaaaattt acttactctg atacttaccc accccaaagc aaaaagcctt
     2401 ggtttcaagg cttttcagta tccctttcgt tcaaaggttt ctaggctatt acgagcagag
     2461 caacacactc gacgtgtgtt gtctggtcct tgagaataca tttttttatc ctcgtcactg
     2521 ttttgaggac acaatttatc tgtttctagt ttagcatttt tacgtttatt tttgaattgt
     2581 tttccatcct tgttttgagt ttcacctgtt ttgaatatta tggttaacat tgcaggatca
     2641 atttctccgt cgctattaac tccaccaaca ataattttct caacaatact ttcaaatact
     2701 gcacgatcaa actctgtaag cattttctgt gaagatagca agtgcttaaa ctcagttagt
     2761 cttttcttta catcgatttc tgattttaag gttgtttcaa tatttacctt ctcttctgct
     2821 agaaactctt tctctgagga aatttcgttg tacttttcat tgtatatggt atcgctaatt
     2881 ttcccttcga gccttagatt tacaagattt tcctcttttt ttagtaatgt tcgaagtttg
     2941 tttgtaatcc tttttagttc cttgttaagg ctattgtcat tcaattcaga ttcaatcgtt
     3001 tcaagtaagt ctgtcattaa gttttcattt gaatgataga gttgtctgta agcttccatg
     3061 aaagccccct caatagcgag ctcttctaat cctttactat gcttacaaaa tttctttcct
     3121 ttctttatgg atgtaacgca gtgccacaca actttgcgat aatctgaacg gcagtgccaa
     3181 cttcttctag ataatattga accacaaaaa ccacattcta acatgctact aaaagcatac
     3241 atttttgaat agcgttcacg ttttccattt acattcgcaa ctgtcttttt atttcctgcc
     3301 ctacgcaacc taatctcctg agctttctca aagtcttctt tagatattat aggttcatga
     3361 ttatctttga tgtagtactt atcttcttca ccaaaattac ttaaacgtct cttgcttatt
     3421 ggatccactg taaaagtttt gcccattaga atatcgcctt tatatttttc attctttatg
     3481 attcctaaga ctgttgtatc attccaatga tctaatcctc tcggcgattt gtaacctagt
     3541 tcatcaagtt cccttgcaat tacttttcct ccaatacctt ctaagtacct ttcaaagata
     3601 tagcgaacta ttttggcttc ttttttattg attgaaagtt gcttggtttc tacatcatag
     3661 tcatacccca agcatccttg aaaccctatc aattcaccac gttgcatctt catttttaat
     3721 ccctttttca catgtgcaga tgtattttct acttcttgct gtgcaacaga gctaagaatg
     3781 gttagaagta attctccatc catagtaagg gtatcaatgt tctcttcctc aaaatatact
     3841 ccaatttgca tatcctttaa cattctgaca tacttcaaag tatctaaggt gtttcttgca
     3901 aatctagcaa ttgcttttgt tacaatataa tcaatttctc catttacaca atcattgatt
     3961 aagcgttgaa aatcttgtct ctttcctact tgagttcctg aaatcccctc atctgcatag
     4021 atatcagcga gttcccaatc tgaacgctga gaaatgtaat ctttataatg tctcacctga
     4081 gattcataac tgttcttctg atcctcacta tcagaactca ctcggcaata agcagcgacc
     4141 ctttttaaat gtctttcaac attgacacct ctacgtctaa ctaaagaatc agcttttatt
     4201 atttcaacat tatttgtttt cataactgaa attcctcctt ttgattctat attaccgcgt
     4261 tcaaataaaa aggtcagcta ttttgtgtac ttaatacgca gtttgtttct gattttttca
     4321 tactcatttt tagatagcaa ttctttagag tataaaaaat taagcattga taactgcaga
     4381 ctatatttta caactgtttg ttccaaaatt tttcctcctg ataacgtgat actcaattat
     4441 ttcctccgtg ggtatgattg gttaaatcat tcatgggcat ctcacccgct gtccttttac
     4501 gctagcagcc tgaacgtgca gaagtatcat tatccttaat ttgtgtcttg gcaaatagag
     4561 taccacccta ttttctagct gactaatatc gctcatatcc tcgcgaatca aactaaactg
     4621 ctcaacaaat aagaaagtcc cacctcggtt tattcagttg tcaatgaaca aatcaaggag
     4681 agactatttt aatttacgtt taattctatt aggacaatct gttagaccta acaaataatc
     4741 tgtactaacc tggtacaggt ccgaaagttt tataagataa tcaatggata gctgacgtct
     4801 accagcttca atcttagaat aggcagactg actgcaagat aaataattag caagatattc
     4861 ttgcgtaaag tcacaatcct ctctcagatc ccgcacacgc ctatacattc gttcactcct
     4921 aaagccatta tagtcaactt ttgaacgaat cttaaaattt atgccaactt ggcataaacc
     4981 acttacttaa aaagcaatat cagtttttta gtacttttta tagtagtacg gacacagata
     5041 acctccttcg tatattcata caatactttg agcagaaaat ctccaaattt ccattattca
     5101 caatagcaaa accattatgg ataattctac tctggctaaa acaatattcg atatatacag
     5161 tgggagtcac gtctcacctg gaatgattaa aaagttttat tttataatag gttagataac
     5221 aagtatattg aataagcatt aatacttaga acgtatctga attattgtga acatatttct
     5281 agcatattat gaagcaaaaa atttagtaaa acaacaagcc ttctaatcaa tagaaagtaa
     5341 tagtatttga atcacgtgaa gataatttga tcttaagaat ttttattaat tcaaaaaaat
     5401 caatattccc aatattcttt atagcttcct cttgcccaac tatatatgaa gccgagtaaa
     5461 acggtggtaa ctcttgaaat attgtagtaa aactgtctgt tactgatatt ccaaatgtgc
     5521 taaaaataga acgatacagt tcctcttttg aggacttagt cctatatttt ttgaaatttt
     5581 taaatagttc gatttgaaaa tttagcttac agctattttc tatcaataaa ttagtcaata
     5641 atcttttata ttcatcaagt tcttccttta ttaaattgtt acaaatggca ctctcaataa
     5701 aatgtgcaag cccctcttct gcttcaagag atagaataat atcgtatttt gtctgataat
     5761 agttcaaaat tacatgtgaa atctcatgaa gaatatagat ttcactaaat gcttcttttt
     5821 ttaaattcgg aaacaccaaa aaacttgtct cacttaatgg caaatgtaca catctaagtg
     5881 tatctatatc agcataaata atattatttt cgactgtacg cttcacatat gaaaaaagtt
     5941 tcaaatcaat attttttatc aaaaataaaa tattttcaat tgatagattt tcaaaaatat
     6001 cattagattc aaattttgta tagcgatacg ccaaacaatc tatttctttt attgttctta
     6061 acccattttc ttcacgccac acattataat caaaatgatt ttgaaaaaga aatttattta
     6121 atttggtatt atttagatat acatattgat tgttttgtcc ttttggaaaa tcaccgccaa
     6181 tttgtggaat attcactgga aataagttaa taattttttg ttgctcagaa atagataacc
     6241 ggtgcacctc gtctatactt ttattcaacg ttgctaaata gccttccagt tttttatcct
     6301 ccaagaaatc ttttaaaaat actaatgagt tatactgctc tataactgag tatccgattc
     6361 ttctcctatt ttttgctttc atttgcaaat ctcgcaccca ggtatattaa tatgcaagcg
     6421 atagctatag caaaaattag ccaaaccttc aaaataggaa ttaaatcaga cagtaatcca
     6481 ttgataaata taaataagct cattatgaat gactcaatcg cataatatag tgatacaatt
     6541 gtagttctat gttcattatt aaccacaatt tcattgtgaa gagtaatatt aatcctgatt
     6601 gataaaattg taaacgttaa tatgtaaagc aaaaataaca aaagggtcag catggacttt
     6661 tgtagcactc caataaatat gattaacaaa atattaataa acaataaagt agtaataaat
     6721 attctgttat tcgtaatttt ctggattttt tctgaaataa atcctccaag cattccgaat
     6781 aaggaaataa aaatccaaat atagccagaa agatctgtga aaatcgaatc atgaaatacc
     6841 tcttgccact gatttgcagg accaacatct agtaccgctg gcaacaacat tagtactaaa
     6901 ataagcttaa gctcttttat caatactaat tccgctaatg tggacttaag agtttgaaat
     6961 gaatcaataa aaatatttga aaaatcaatt tttttcttat ctgttgaaga agaaaatagt
     7021 agaagtacaa ataaacatag aaaaacaaaa gttaacacgc ttactataat aggtaatttt
     7081 atatcataat tgaataaaac tttcgaacta acaaaaccga agagcattgt taaaatcgaa
     7141 gatatgattg agcttctcga aaaaagtaga ggaattttct ccttttcgcc tatttcatcc
     7201 aaagaattaa ctacccaact ttgaacagtc ccagattgga aagattcagc tactgcagaa
     7261 ataaatgaag ataataacaa taattgaaaa atattagata acaacaaaag caatcctaat
     7321 gctctcaagc aaaagcttaa aaccaatgta ttcctaactc ctatcgtatc tgtaatagca
     7381 ccacatggta cttctgtcaa gaatgagaat acaaaaaaca aagctaaata tatacctaat
     7441 tgataatgag agaaaccttg tgttaataaa aaaatgtata aaattggatt aaatagtgct
     7501 gtaccaatag aaacaccaga taaaattata aaatatttag ttacaatgct tttcatagtc
     7561 actccaatct ttgttaactg ttacatattc aagaccatat ctatctctag ctaaaaaacc
     7621 ataatctacc aaatatctcc tcaatattgc atagtcgtca tagtacattt ttaaaatact
     7681 atttatttct ttttcagtaa aataattccc tctattgact atctgctcat ataaaaaaca
     7741 aaacactttc tcctttgaac gtttcttttt tggaatagtt atcaatttac cattagaaaa
     7801 aaaattgtta acatcactca cgactatctc cttcgttatt aaaaattatt tttttataat
     7861 aaagtgctaa atatttataa atctcatttt ctaatgttgg aatattacca actaatctaa
     7921 tattactcat atgaattaat gacatatggt ccaaatcgcc ataattttca attattggaa
     7981 atctattaat aaatcctaaa tctgactcca ttattctggt aactttatcg ctctttctcc
     8041 tatcctcttt tcctactata aaatctttta aaa
//
LOCUS       DATUCG010000111         7827 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.113, whole genome shotgun sequence.
ACCESSION   DATUCG010000111 DATUCG010000000
VERSION     DATUCG010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7827
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.113"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            <1..1011
                     /locus_tag="VCA20_001915"
     CDS             <1..1011
                     /locus_tag="VCA20_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEP1835675.1"
                     /translation="VTEQVYFLITTAGTDTHSICYETHQKAKDILEGRKVDPTFYPVI
                     YGADEEDDWTDPKVWKKANPSLGITVGIDKVKAACESAKQNPGEENSFRQLRLNQWVK
                     QAVRWMPMDKWDACSFAINEDELEGRVCYGGLDLSSTTDITAFVLVFPPIDEEDKFIV
                     LPYFWLPEDTLDLRVKRDHVPYDIWERQGFIQTTEGNVVHYGYIESFIEKLGERFNIR
                     EIAFDRWGAVQMVQNLENMGFTVVPFGQGFKDMSPPTKELMKLTLEQKIAHGGHPVLR
                     WNMDNIFIRTDPAGNIKADKEKSTEKIDGAIATIMALDRAIRCGNQNTESVYDSRGIL
                     FM"
     gene            1309..2628
                     /locus_tag="VCA20_001916"
     CDS             1309..2628
                     /locus_tag="VCA20_001916"
                     /inference="COORDINATES: protein
                     motif:HMM:NF024571.1,HMM:NF025019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEP1835676.1"
                     /translation="MLHYERKLKTRNIYLNQLIAFKDKEPVKVITGIRRCGKSSLLKL
                     MQEYLLNSGVKQDQIIAINFESLEFQEMNYKELYEYVKKKIPTTKRAYLFFDELQRIE
                     RWEDAINSFRVDFDCDIYITGSNSYLLSSEYSTYLSGRYVEIKMYPLSFKEFIDFHGY
                     KLKEYKTPIGEKRKRAVNENDEIVEIRDLFDAYMRYGGMPGIADVGLEQDKAMTLLDG
                     VYSTVVVRDILEREKRRGLRQITDAELLRKIILFLADNIGNNTSLNSVSNTLVSENMI
                     QNRERQGKPATQTIASYVGALKESYMFYDVKRFDIKGKEYLRTLGKYYIVDIGLRNYL
                     LGFRDRDRGHSLENIVYFELLRRGFDVAIGKIDNLEVDFIATSANEKMYIQVTESMKN
                     ELVRERELKSLQKIHNNYKKMVITLDKALDDEYDGIQSIDVIEWLLK"
     gene            2706..4031
                     /locus_tag="VCA20_001917"
     CDS             2706..4031
                     /locus_tag="VCA20_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001933576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEP1835677.1"
                     /translation="MGILSGLFKSRDKPTNRTNGSAYSFLMGGTPSGRRVNERSAMQM
                     TAVYSCVRILSEAVASLPLHVYLRTDIGTEKAIDHPLYKVLHDEPNPEMTSFIFRETM
                     MTHLLLWGNAYAQIIRNGKGEVLGLYPLMPDRMKVDRDERGQIYYEYLVSDGDVSSTK
                     QGAVKLKPSDVLHIPGLGFDGLVGYSPIAMAKNAIGMAIATEEYGAKFFANGATPSGI
                     LEYPGTVKDPQAMRDSWSKGFSGSNSHKVAILEEGMKYTPISISPNEAQFLETRKFQI
                     NEIARIFRVPPHMVGDLEKSSFSNIEQQSLEFVKYTLDPWVARWEQSIVRRLFTEEEK
                     KKYYVKFNVDGLLRGDYQSRMNGYAIGRQNGWMSANDIRELENLDRIPSEEGGDLYLI
                     NGNMLPLNRAGAFAGDEGKEEEPNEEVLEVEERSEKPKRRGSDRTHPVP"
     gene            3952..4689
                     /locus_tag="VCA20_001918"
     CDS             3952..4689
                     /locus_tag="VCA20_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001933578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HEP1835678.1"
                     /translation="MKKFWKWKNGVKNQNEEEVTERILFLNGTIAEESWFDDDVTPQL
                     FKEELNSGSGNITVWINSPGGDCIAAVQIYNMLMEYKGDVTVKIDGIAASAASVIAMA
                     GTKVLMSPVSMLMIHNPMTLAFGNAGEMEKAIAMLDEVKESIINAYEIKTGLSRAKLS
                     HLMDSETWMDANKAVELGFADDIIKRSETTDTQVPQVSMMYQEAQVVNSLMYKLASKC
                     KIENKETNIGVKADELMDRLFLMKNWR"
     gene            4696..5871
                     /locus_tag="VCA20_001919"
     CDS             4696..5871
                     /locus_tag="VCA20_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006268656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEP1835679.1"
                     /translation="MSKILEMIEKRNKAWEGAKAFLESKRDKDGLISEEDALSYDEME
                     KKVHNYSLEIERLQKIEEMDKELSKPMSDAIVSRPMKVEDKPEKKGRARDEYKESMLT
                     ALRTNFKKVSDILQEGVDADGGFLVPVEYDKRLIETLEEENIMRGLATKITTSGQHKI
                     NVAMSDPAAAWIEEGGALNFGDSKFAQVLLDAHKLHVAVKVTEELLYDNAFKLEDHIL
                     GAFGKALANAEEDAFLNGDGNGKPTGIFNKKDGGTFLKEITAIKTDDLIDLVHSLKRS
                     YRKKTAFIMNDKTIAQVRKLKDNNGAYIWQPSYQEGEPDRILDYPVKTSAFAPENAIA
                     FGDFSYYNIGDRGARSFKELTELFAGNGMIGFVAKERVDGKLVLKEAVQILPIKATA"
     gene            5894..6172
                     /locus_tag="VCA20_001920"
     CDS             5894..6172
                     /locus_tag="VCA20_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001933584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HEP1835680.1"
                     /translation="MVVSLDEMKNYLRVDTSEDDDLISTLIKSSEKMCLAIVRKEEDE
                     IIKDSFEEYKVAVLYGTAYLYEHREEADHHELTITLRSMLFGVRKADF"
     gene            6172..6504
                     /locus_tag="VCA20_001921"
     CDS             6172..6504
                     /locus_tag="VCA20_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001933586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEP1835681.1"
                     /translation="MKISLLNERILIEKSKVIIDAIGNHRNEWSKYYSCYATISNESP
                     QEETSSGATWDESKIDFTIRYSKEVSILSSVGYRVIFHNDIYEIEGIDHMNYKKKSMK
                     LHCRRVER"
     gene            6501..6880
                     /locus_tag="VCA20_001922"
                     /pseudo
     CDS             6501..6880
                     /locus_tag="VCA20_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019116073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
     gene            6873..7205
                     /locus_tag="VCA20_001923"
     CDS             6873..7205
                     /locus_tag="VCA20_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001933591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835682.1"
                     /translation="MDKLLEIIEKIGFPSAYHHFAEGESPNPPFLIYILPVSDNFSAD
                     GRVYFKANEVHIEVYTDYKNLDIEKKVEVVLDEYDIFYNKSEVFIESEKLYEVLYIFE
                     MEVKTNGK"
     gene            7195..7683
                     /locus_tag="VCA20_001924"
                     /pseudo
     CDS             7195..7683
                     /locus_tag="VCA20_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008817782.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
BASE COUNT         2795 a         1104 c         1757 g         2171 t
ORIGIN      
        1 gttaccgaac aagtctattt tcttatcaca acagcaggaa ctgatacgca ttctatctgc
       61 tatgaaaccc atcaaaaggc taaggatatt ttggaaggga gaaaagtcga ccctactttt
      121 tatcctgtaa tttatggggc agatgaagaa gatgactgga cagatcctaa agtttggaag
      181 aaagcaaatc catcacttgg aattactgta ggaattgata aggtaaaggc tgcctgtgaa
      241 tctgcaaaac aaaatccggg tgaggagaat tcctttagac agcttagact taatcaatgg
      301 gtaaaacaag cggtaagatg gatgccaatg gataagtggg atgcctgttc ctttgctata
      361 aatgaagatg aacttgaagg aagagtatgt tatggaggac ttgacctttc ctctacaaca
      421 gatatcactg catttgtact ggtgtttcca ccaatagatg aggaagataa gtttatagtg
      481 cttccttatt tttggttacc ggaagataca cttgatttaa gggtgaagcg tgaccatgta
      541 ccctatgata tttgggaaag gcaaggtttt attcaaacca cagaaggaaa tgtagttcac
      601 tatggatata tagaaagttt tatagaaaaa cttggagaaa gatttaatat aagagaaatt
      661 gcctttgata gatggggtgc tgttcagatg gttcagaacc tcgagaatat gggatttacc
      721 gtagttccat ttggacaggg ttttaaggat atgagtccgc caactaagga acttatgaaa
      781 ttaacactag agcaaaaaat agctcatggt ggacatcctg ttcttagatg gaatatggat
      841 aacatattta ttaggacaga ccctgcggga aacatcaaag cagataaaga aaaatccaca
      901 gaaaagattg atggtgcaat tgcaacaatt atggcacttg atagggcgat taggtgtggg
      961 aatcaaaata cagaaagtgt atatgacagc agaggtattt tgtttatgta gtttcaaaac
     1021 ttgcttttgt gcgttatttt tgcttttata atgcacagaa gaaagtacta aatataaaat
     1081 aattgcctaa actcttaaca atcgcctaaa aatatgctgt aattaggcga aagaaagagg
     1141 gagtgtattt atgtaggtat ctgtttttga aatatagata gggaagagtc atccttattt
     1201 cagaaaaatt gattttctga aataagatta agtttaggga attgatacct caaacttgat
     1261 aaaaataaaa gtttagggta taatgtaatc aaaaaggagg gatatggtat gttgcactat
     1321 gagagaaagc taaaaactag aaatatatac ctgaatcaat tgattgcatt taaagacaaa
     1381 gaacccgtaa aggttataac aggaattaga agatgtggaa aatcaagttt attaaaacta
     1441 atgcaggaat atcttttaaa ttctggagtt aaacaagatc agattattgc aataaatttt
     1501 gaatcccttg aatttcaaga gatgaattac aaagaattat atgaatatgt aaagaaaaaa
     1561 attccaacta caaaaagggc ttatttattt tttgatgaac ttcaaaggat agaaagatgg
     1621 gaagatgcta taaattcatt tcgagtcgat tttgactgcg acatatatat aactggatcc
     1681 aattcatatc tattgtcatc ggaatactct acatatttat ctggcaggta tgtagaaata
     1741 aaaatgtatc cattatcatt taaggaattt atagattttc atggatataa attaaaggaa
     1801 tataaaacac caattgggga aaagagaaaa agagccgtaa atgagaatga tgaaattgtt
     1861 gaaatcagag acttatttga tgcctatatg cgatatggtg gtatgcctgg gatagcagat
     1921 gttgggcttg aacaagacaa agcgatgact ttactcgatg gggtatattc aactgtagtt
     1981 gttagagata ttcttgaacg tgaaaaaaga agaggattaa gacaaattac tgatgcagaa
     2041 ttacttagga aaatcatatt atttttagca gataatattg gaaataatac atctcttaat
     2101 tcggtaagca atacgcttgt atctgaaaac atgatccaaa atcgtgaacg acaagggaaa
     2161 ccagcaacgc aaactattgc atcatatgta ggagcattaa aagaatcata tatgttttat
     2221 gatgttaaga gatttgatat aaagggaaaa gagtatctaa gaacacttgg caagtattat
     2281 atagtggata ttggactaag aaattattta cttgggttta gagatagaga cagaggacat
     2341 tctttagaga atatagttta ctttgaattg ctaagacgcg ggtttgatgt ggcaattgga
     2401 aaaattgata atttagaggt agattttatt gctacaagtg caaatgaaaa aatgtatatt
     2461 caagtaactg aaagcatgaa aaatgaattg gtaagagaaa gagaattaaa atcgttacaa
     2521 aaaattcata ataattataa aaaaatggtt attactttag ataaagcact tgacgatgaa
     2581 tatgatggaa ttcaatctat tgatgtaatt gagtggctat taaaataaaa caatgagaga
     2641 acataacgat aggcatctac ttcagcagta ggtgcttttc ctatgtgaaa aattaggagg
     2701 aacatatggg aatattaagt ggtttattta aatccagaga taagcctact aacagaacaa
     2761 acggaagtgc atacagcttt ttgatgggtg gaacaccatc cggaagaaga gtaaatgaac
     2821 gctcggctat gcagatgaca gctgtatata gctgtgtccg aatactctct gaagcagtcg
     2881 caagccttcc acttcacgtc tatttacgga cagatatagg aactgaaaaa gcaatagacc
     2941 atccgctata taaggtgctt catgatgagc cgaatcctga aatgacaagt ttcattttta
     3001 gagaaactat gatgacacat ttacttttgt ggggtaacgc ctatgctcaa ataatcagaa
     3061 acgggaaagg tgaggtatta ggtctttatc cacttatgcc tgacagaatg aaggtggata
     3121 gagatgaaag aggccagata tattatgaat atcttgtcag tgatggggat gtatcctcta
     3181 ctaaacaagg ggcagtaaaa cttaagcctt ctgatgtgct tcatattccg ggacttggtt
     3241 ttgatggact tgttggatat agtccaattg caatggctaa aaatgctata ggcatggcaa
     3301 tcgcaacaga agaatatggc gctaagtttt ttgctaatgg ggcaacacca agtggaattc
     3361 tagaatatcc tggaacggtg aaagacccgc aggcaatgag ggatagctgg tcaaaaggct
     3421 tttcaggaag taactcacac aaggtagcaa ttttggaaga aggaatgaag tatacaccaa
     3481 tttccatttc tcccaatgaa gcacagtttt tagaaacgag aaaatttcaa attaatgaga
     3541 tagctaggat tttcagagta ccacctcata tggtaggtga ccttgagaag tctagctttt
     3601 ctaatatcga gcagcaatct ctagagtttg tgaagtacac tcttgatcct tgggtagcaa
     3661 gatgggagca atccattgtt cgaaggcttt ttactgagga ggaaaagaaa aagtactatg
     3721 tcaaattcaa tgttgatgga cttcttcgtg gagattacca atcaagaatg aatggctatg
     3781 ctatcggcag acaaaacgga tggatgtctg ccaatgatat tagagaatta gaaaaccttg
     3841 accgtattcc tagtgaagaa ggcggggatt tgtatctcat aaatggaaat atgctcccac
     3901 taaaccgtgc cggagcattt gcaggtgatg aagggaagga ggaagaacct aatgaagaag
     3961 ttttggaagt ggaagaacgg agtgaaaaac caaaacgaag aggaagtgac agaacgcatc
     4021 ctgttcctta atggaacgat agcagaagaa tcatggtttg atgatgatgt gactccacag
     4081 ctatttaagg aagaattgaa ctcgggtagt ggaaatatca cagtctggat taactctccg
     4141 ggaggtgact gcatagcagc agttcaaata tataatatgc tgatggagta taaaggcgat
     4201 gttacagtaa aaattgatgg cattgcagca agtgcggcat cggttattgc tatggcaggt
     4261 acaaaggtac ttatgagtcc tgtatctatg cttatgattc acaatcctat gaccttagca
     4321 tttggcaatg caggagagat ggaaaaagcc atagcaatgc ttgatgaagt aaaagaatcc
     4381 attataaatg cctatgaaat taagacaggg ctgtcaagag cgaaattatc tcaccttatg
     4441 gattcagaaa cttggatgga tgccaataaa gcagtggagc ttggctttgc agatgacatc
     4501 ataaaaagaa gtgaaactac agacacacaa gtaccacaag tttctatgat gtatcaagag
     4561 gcgcaggtgg tcaattcgct gatgtataaa ttggcctcaa aatgcaagat agaaaacaaa
     4621 gaaacaaata taggtgttaa ggcggacgaa ttaatggacc gtcttttttt aatgaaaaat
     4681 tggaggtaga acactatgag taagatttta gaaatgattg aaaaacgtaa taaggcatgg
     4741 gaaggtgcta aggcttttct tgagagtaaa cgtgataaag atggccttat ttctgaagaa
     4801 gatgctttaa gctatgatga aatggaaaag aaagtccata actacagttt ggagattgag
     4861 agactgcaaa agattgagga gatggataaa gaattatcta aacctatgtc tgatgctatc
     4921 gtaagtagac caatgaaggt tgaagataaa ccagagaaga agggcagagc aagagatgaa
     4981 tacaaggaat ctatgctcac tgcacttaga actaacttca agaaagttag tgatattttg
     5041 caggaaggtg tggatgctga tggtggtttt cttgttccag ttgaatacga taagagactg
     5101 attgaaaccc ttgaagaaga aaatatcatg cgtggattag ccacaaaaat aactacaagt
     5161 ggtcagcata agattaatgt agccatgtca gaccctgctg cagcttggat tgaagaaggt
     5221 ggtgcattaa actttggcga ttctaaattt gcacaggtac ttcttgatgc acataaactt
     5281 catgttgcag taaaggtaac agaggaactg ttatatgata atgcctttaa gttagaggat
     5341 catattttag gtgcctttgg taaggctctt gcaaatgcag aagaagatgc cttccttaat
     5401 ggtgatggaa atgggaaacc gaccggtatc tttaacaaga aagacggtgg aactttccta
     5461 aaagaaatta ctgctatcaa gaccgatgat ttaatcgacc ttgttcattc cttgaaacgt
     5521 tcctaccgta aaaagactgc attcattatg aatgataaga caatcgctca ggtgagaaag
     5581 cttaaggata acaacggtgc atatatttgg cagccatctt atcaggaagg tgagcctgat
     5641 agaattctag attatccagt taagacttca gcatttgcac cagaaaatgc tatcgcattt
     5701 ggtgacttta gctattacaa cattggcgat agaggggctc gttctttcaa ggaacttact
     5761 gaactatttg ccggtaatgg catgattggt tttgtggcaa aagaaagagt tgatggcaag
     5821 cttgtattaa aagaggctgt tcagattctt ccaattaagg caacagcgta attaatgaaa
     5881 gggggtgctg attatggttg taagtcttga tgaaatgaaa aactatctaa gggtggatac
     5941 aagtgaagat gatgatttaa tcagcaccct tataaagtct tctgagaaga tgtgccttgc
     6001 tatagtaaga aaagaggaag atgaaatcat aaaagatagt tttgaagaat ataaggtggc
     6061 agttctatat gggactgcct atctttatga acatagggaa gaagctgacc accatgaact
     6121 gacgattact cttaggtcca tgctatttgg agtaagaaag gcagacttct aatgaaaata
     6181 tcactattaa atgaacgcat tcttatagaa aaaagcaaag taatcattga tgcgattggc
     6241 aatcacagaa atgaatggag taaatactat tcctgctatg caacaattag taatgaaagt
     6301 ccacaagaag agactagtag tggcgctaca tgggatgaaa gcaagattga ttttactatt
     6361 cgttacagta aggaggtatc gatcttatct tctgttggat atagggtcat ttttcataat
     6421 gatatttatg aaattgaggg gattgaccat atgaactata agaagaaaag tatgaaactg
     6481 cactgcagga gagtggaaag atgagcaagg taaaaattga tagtctttca tctgaagtga
     6541 tgaaagaact agaaaaatat gctgatgtta caactgaaaa agtaaaaaag gcagttaaaa
     6601 atgcaggaaa gactatgcgt gatgaaataa gtagcgctgc tccaagtgat acaggtaagt
     6661 atggtaaaag ctggacggta aaaactgtga gagaaacatc aagcagtctg gaactggtcg
     6721 ttcattctaa aatagatatc agcttaccca tcttcttgag tttggtcatg caaagcgtgg
     6781 tggtggcaga gtatctgcaa gacctcatat ttcagcagct gaagaaaagg cgataaaggt
     6841 atttgaagaa gaaataaagg aggctatttc aaatggataa gctactagaa attatagaaa
     6901 aaattggatt tccgagtgct tatcaccatt ttgcagaagg tgaatcacct aatccacctt
     6961 ttcttattta catcttgcca gtaagcgata acttttcagc ggatggaagg gtgtatttta
     7021 aagcaaatga agttcatatt gaagtttata cagattacaa aaatctagat atagaaaaga
     7081 aggtagaggt cgtactagat gagtacgaca ttttttataa caaatcagaa gtctttatag
     7141 agtctgaaaa actctatgaa gtcctatata tttttgaaat ggaggtaaag acaaatggga
     7201 aataaggtaa agtataacct taaaaatgtt catgctgcaa agctgaaaaa agatacaagt
     7261 ggtgcattta cttacgaaaa tccaaaggca ataccgggtg ctgtaagtat tagccttgat
     7321 gcagaaggtg aatccagtcc tttttatgca gatgggattg tttactttag gtctactgcc
     7381 aataatggat atagtggtga ccttgagatt gcactgattc cggaatggtt taggacggaa
     7441 attctaaagg aagaactgga taaaaatggg gtgcttgttg aaaaggcaaa tgtatctgaa
     7501 acagctaaat ttgcactgct atttgagttt gatggagatg tgaatgcaat aagacatgtc
     7561 ttatataact gctcagcatc tcgtccatca attgaatctg aaaccaaaga agatacgatt
     7621 gaaccaggaa cagaaacatt atcccttaca gcagatccaa gagaagatgg acttgtgaaa
     7681 aatccccata aacttatttt acgaggagaa agagagggag aatatgctct ggatattgac
     7741 ggacgtagag gctcttacag actaatcgtc tgctttgatg aggagaaaga cacagtattc
     7801 agaacgtaga caaacatcgt ttcggtg
//
LOCUS       DATUCG010000112         7801 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.114, whole genome shotgun sequence.
ACCESSION   DATUCG010000112 DATUCG010000000
VERSION     DATUCG010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7801
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.114"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            854..2764
                     /gene="ltrA"
                     /locus_tag="VCA20_001925"
     CDS             854..2764
                     /gene="ltrA"
                     /locus_tag="VCA20_001925"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001129116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEP1835683.1"
                     /translation="MAKRIVNKAERNAERYDAKEIGYQLYEDSLNGKRFDRLMPMIVS
                     DQNIILAYRNICKNNGSKTPGTDGKTIVEIQKLPIEMVIKTIRNKLNYYQPKNVRRVE
                     IPKENGKARPLGIPSIWDRLIQQCVLQILEPICEAKFHERNNGFRPYRSTQNAIAQCY
                     KMAQIQNLHFVVDVDITGFFDNIDHSKLIRQLWGLGVQDRKLIIIIKQMLKADILFND
                     IVITPETGTPQGGILSPLLANVVLNELDWWVANQWEMFKIKEGSTGYEFTKVDNEGNI
                     LTIDRTQKWNKLRAKTGLKEMYITRYADDFKIFCRDYATAVKVMKATKLWLAENLHLR
                     TSDEKSGITNLRKNYTTFLGIKFKVVPKGNKWIIRSHMADKSKVKVISKLGSVWKDIK
                     NPSKQSELDKNISLYNAMVMGMHNYYCMATLVSSDFAEIAFKVTGKSNGMNHNKRCFP
                     IEKQGEITSKYILDKYGKSKQCRWINGRMIVPVGYVSYEYPKYKRREVNKYVRKYSDA
                     ENCISFEVMKYMMENAHLYPTLEMADNALSRYIAQKGKCAVTHNALTIEYMVCEHIKP
                     CKGERNDTYRNLIILSKEVSDLVGEENSNKVSKLLKNLPLTKEMQDKINKLREHRELE
                     MIQFEDYIGTKK"
     gene            2787..3410
                     /locus_tag="VCA20_001926"
     CDS             2787..3410
                     /locus_tag="VCA20_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001253103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HEP1835684.1"
                     /translation="MMGNYHARCGAGEKPEVVTPEAYLSLFGEIPDFAEQTSTVRSRN
                     MSLVPILQNIAQLQGLYKEKEAWKTILGNCDSLLYLGGNDEETFKFMSGLLGKQTIDV
                     RSTSRSYGQTGSGSTSHQKIARDLMTPDEVGNMKRDECLVRIAGVPVFKEKKFFPLKH
                     KNWQYLADKESDERWWHYHIDPLKTEEVPFEPSDHKVRDLSKESTLH"
     gene            3428..3670
                     /locus_tag="VCA20_001927"
     CDS             3428..3670
                     /locus_tag="VCA20_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835685.1"
                     /translation="MNQKLTGFVYGVDASSMFAQAMALLQKGMVATGAFLVVMGIINL
                     STNIKDGGPGVRNAILEIVGGVMVGAAGAFITQITI"
     gene            3687..4541
                     /locus_tag="VCA20_001928"
     CDS             3687..4541
                     /locus_tag="VCA20_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbL"
                     /protein_id="HEP1835686.1"
                     /translation="MNNTLPSAFAFLASEKISSDSLFEGFQVDLESTANLVKSLADFN
                     PTVWSYMTAITKGIMQPLGVAILAVVLVLEFSKMAKKIANSGGAMTFEAIAPMIVSYI
                     MVAVVITNTTVIVEAIVAIASYVIEQVASLVTNGGANYDTISGIKGSGIVGKMIIGFF
                     AILIWLVRMTSIMVVNILITIRFIQLYLMIPFAPVTIPTFLSDDWRSVGIGYLKNIMV
                     YAVQGILIFLIVSLVPLFESAGKIAVSNGAGVMESLAIMFGGLVQAILLIIALVGSQR
                     TARSILGM"
     gene            4603..4956
                     /locus_tag="VCA20_001929"
     CDS             4603..4956
                     /locus_tag="VCA20_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HEP1835687.1"
                     /translation="MNTRVFKDISKVQHRAWLGFTTRQVIFVFPAVALTILVLGLNLF
                     YWQFGDWFVYGFVFSFTIPLMLFGVYRPNDLPFETYLKYRFHYELTVPDRTFTGRKGD
                     QREKIKTLNETKDLF"
     gene            4913..6406
                     /locus_tag="VCA20_001930"
     CDS             4913..6406
                     /locus_tag="VCA20_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000734148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HEP1835688.1"
                     /translation="MKKLKHSMKPKTSSNDKNQKTKIQKQEIRPSTVNTLAYQGLFQN
                     GLMQVSPSYFSQTYLLGDVNYQTVGLDDKGAIVEKYSDLINSLDDQTNFQLTIFNQKV
                     NLEKFRKSILYSLQKDGFDAYRDELNRMMDANLEAGENNFSAIKFLSFGKSDQTPKLA
                     FRSLSQIGEYFKSGFSEIDVSLGLLGGEERVNVLADMLRGENHLPFSYKDLTLSGQST
                     KHFIAPTYLSFKHKNHIELDERLLQIVYVRDYGMELGDKFIRDLMQSDLEVMISLHAK
                     GSTKSETMTKLRTKKTLMESQKIGEQQKMARTGIYLEKVGHVLENNINEAEALLQTMT
                     QTGDKLFDTVFLIGVLADTEDQLKQSLDIIKQVAGSNDMIIDNLTYMQEAAFNSLLPF
                     GKNYLEGVSRSLLTSNIAVNAPWTSVDIQDKGGKFYGINQISSNIISIDRGKLNTPSG
                     LILGTSGAGKGMATKHEIISTKLKESDSDTEIIIVDPENEVRQEVVL"
     gene            6936..>7801
                     /locus_tag="VCA20_001931"
     CDS             6936..>7801
                     /locus_tag="VCA20_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017284794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase N-terminal
                     domain-containing protein"
                     /protein_id="HEP1835689.1"
                     /translation="MNDKHSTTAPAEKLSHKQLLAKQWKSIDWKRAEQEVNRLQIRIV
                     KATQAKHTNTVKRLQYLLTHSFYAKALAVRRVTTNKGKKTAGIDGELWTAPAQKMEAL
                     LSLTDKGYKASPLRRVYIDKKGKKKKRPLGIPTMYDRAMQALYALALEPVAETTADTK
                     SFGFRKGRSCQDACEYIFTALSRKASPQWILEGDIRGCFDNISHDWLLENIPMDKSIL
                     KQFLKAGFVFKGELFPTEDGTPQGGIISPILANMALDGLQQVLSDRFHTNRLGKIDLR
                     FKNSHKVNLVRYA"
BASE COUNT         2544 a         1437 c         1743 g         2077 t
ORIGIN      
        1 caactgccga ttcatgttag aagctatctg gatgagtttg cgaatggtgt gacacgttct
       61 acgcccaaag cggtaggaat aaccgacaaa agcgtagcct aaagtcacaa ataatttaat
      121 aatgtggttt tagagaactg cataactaaa atttcaaatg aaggggtaac gccctgaagg
      181 gaattcgcta atactccgaa tagcgttagt tgaatacaga tgactaaggt tataagctcg
      241 gagaagtcgt gcgaagcaca ccctaaagta gaaatactag gaaagatacc tagaggtagg
      301 tgttgtgtgt gacagtgcgg gcggggttgc catatggtta gaatgaccaa ataggcggtc
      361 tgacaaaatt ccgaatgtac ggctctaaac gaatggctgg tagaaatgcc agtgtaccaa
      421 tgggtacgca agtggggtaa agtaaaagtt gttagtatga aataccctct gtgtttacag
      481 gacacaggta tcttgagaaa actcttgaag ataatacttg cagggctaaa ggaatgacct
      541 aagtggtaac aatcctaagt tatgtggaac gtggaaagca accaatacgg gagctgactg
      601 tatcagcgac aaagtaccgt agttgtatcg aaaatgaaaa ggctgaaaga accttttcta
      661 caataagtgc atttatggtt gtgagagcag tggcacagta ccgttgaagc cgtgataata
      721 agcggtggag ggatagccac aagccgatac taaattttct ttcatgtcta tatagtaatc
      781 accaaaagga gattttcata gctgtcgtgg tgcgactaag aaaaaatcat tctccgataa
      841 ggagttggtt actatggcaa aacgaattgt aaataaagca gaacgaaatg cagaaaggta
      901 tgacgctaaa gaaataggct atcagcttta tgaagatagc ctaaacggta agagatttga
      961 taggcttatg ccaatgattg tctctgacca aaacatcata cttgcatatc gaaatatctg
     1021 caaaaataac gggagtaaaa ctcctggtac agatggtaaa acgattgtag aaatacagaa
     1081 attacctatc gaaatggtca taaaaaccat cagaaacaag ttgaactact atcaaccaaa
     1141 gaatgtcaga cgagtagaaa ttccgaagga aaacggaaaa gcaagacctc ttggtattcc
     1201 gagcatttgg gacagactaa tacaacagtg tgtcttacag atactagagc caatctgtga
     1261 agctaagttt cacgaacgaa ataatggttt cagaccatat aggtctacgc aaaacgcaat
     1321 agcacagtgc tataaaatgg cacagataca aaatctgcac tttgttgtag atgtagacat
     1381 tacaggattc tttgataata ttgaccactc aaaacttatc cgtcagttat ggggattggg
     1441 agtacaggac agaaagctga ttataataat caagcaaatg ttaaaagcgg acattctgtt
     1501 taatgacata gttattacac cagaaacagg tacacctcaa ggtggtattc tatctccgct
     1561 actagctaat gtagtgttga atgaactgga ctggtgggtt gctaatcagt gggaaatgtt
     1621 caaaatcaaa gagggaagta caggctacga gtttacaaag gttgataatg agggaaacat
     1681 actcacaata gaccgaacgc agaaatggaa caagctcaga gcaaaaacag gattgaaaga
     1741 aatgtatatt actagatatg cagatgactt caagattttt tgcagagatt atgcgacagc
     1801 tgtcaaggta atgaaggcta cgaagctatg gttggctgaa aatctgcatt tgcggacaag
     1861 tgatgaaaaa tcaggaatta ccaaccttag aaagaactac acgacttttc ttggaataaa
     1921 attcaaagta gtaccaaaag ggaataaatg gattattcgt tcccatatgg cagataagag
     1981 taaagtcaag gttatcagta aactgggttc tgtttggaaa gatattaaaa acccaagcaa
     2041 acaaagtgaa ttagataaaa atataagtct ttacaacgct atggtcatgg gaatgcacaa
     2101 ttattactgt atggcaacgc ttgtgtcatc cgactttgcg gaaatagcct ttaaggttac
     2161 agggaaaagc aacggaatga atcataataa acgatgcttt cccatagaga aacaaggtga
     2221 aattacaagc aaatacatct tggataagta cggaaagtcc aaacaatgta gatggataaa
     2281 tggtcgtatg attgttcctg tcggatatgt atcttatgaa tatccaaaat ataaaagacg
     2341 tgaagtcaac aaatatgtga ggaaatactc agatgctgaa aactgtatca gctttgaagt
     2401 tatgaagtat atgatggaaa atgctcatct ctatcctaca ttagagatgg cagataatgc
     2461 tctttcaagg tatatagcac aaaaaggcaa atgtgctgtt acgcataatg ctttgaccat
     2521 cgagtatatg gtctgtgaac acatcaagcc ttgcaaagga gagcgtaacg atacctacag
     2581 gaatttaatt attctctcaa aagaagtatc ggatttggta ggagaagaaa atagcaataa
     2641 agtaagtaag ctactcaaaa acctaccatt gaccaaagaa atgcaagata aaatcaataa
     2701 gcttcgtgaa catagggagc tagaaatgat acaattcgag gactatatag gcactaaaaa
     2761 gtaagattta gtcggtggaa cgccgtgtga tgggaaacta tcacgcacgg tgtggagcgg
     2821 gggaaaagcc cgaggtggta acaccagagg cttacctatc gctattcggt gaaattccag
     2881 actttgcgga acaaacctca acggttcgct cacggaacat gagtctcgta cccattcttc
     2941 aaaacattgc ccaacttcaa ggactttata aggagaagga ggcttggaaa accattctag
     3001 gaaactgtga cagtttgctt tatcttggag gcaatgatga agagaccttc aagttcatga
     3061 gtggtctctt aggcaaacaa accattgatg tgagaagtac cagtcggtcg tatggccaaa
     3121 cagggtctgg gtcaacgtct catcagaaaa tcgcaaggga tctcatgacc cctgatgaag
     3181 tcggaaacat gaaacgtgat gagtgcttgg tgagaattgc aggggttcca gtctttaagg
     3241 agaaaaagtt cttccccttg aaacacaaga actggcaata cctggcggat aaggagtcag
     3301 atgaacgttg gtggcattac cacattgacc ccctcaaaac agaggaagtt ccgtttgaac
     3361 cctcagacca caaggttagg gatttaagca aagaaagcac actacactaa tagaaaagag
     3421 gaatcgtatg aaccaaaaat taacaggctt tgtttatggc gtggacgcta gttccatgtt
     3481 tgcccaagcc atggcgctct tacaaaaagg aatggttgcg acaggcgcct ttcttgtcgt
     3541 catgggcatt attaacctat caaccaatat caaagatggt ggacctggtg tccgcaatgc
     3601 cattttagaa attgtgggtg gtgttatggt aggtgccgca ggtgccttta tcactcaaat
     3661 taccatttag gagggctagg tatgacatga acaacacatt accttcagct tttgcctttc
     3721 tagcctcgga gaagatttca agcgatagcc tttttgaagg gttccaggtc gatttagaat
     3781 caaccgccaa cttggttaag tcactagcgg actttaatcc gacggtttgg tcttacatga
     3841 cagccattac caagggtatt atgcagccct tgggagtagc tattcttgcg gtagttctcg
     3901 tactcgaatt ttcaaaaatg gccaagaaaa tcgcaaactc aggtggtgca atgacctttg
     3961 aagccatagc acctatgatt gtcagctaca tcatggtcgc ggtcgtgatt accaatacga
     4021 cggttattgt ggaagccatt gttgccattg cctcttatgt tattgaacaa gtggcaagtc
     4081 ttgtcacgaa tggtggtgct aattatgata ccatctcagg cattaaggga tctggaattg
     4141 taggaaaaat gattattggc ttttttgcga ttctcatttg gttggtacga atgaccagta
     4201 tcatggttgt caatatcttg attaccattc gctttatcca actctatctg atgataccct
     4261 ttgcccctgt taccattccg acctttctta gtgatgactg gagaagtgtt gggattggct
     4321 accttaaaaa catcatggtc tatgccgttc aaggtatttt gatttttcta attgtatctc
     4381 ttgttccctt gtttgaatcg gctggaaaga ttgccgtatc aaatggagct ggagtgatgg
     4441 aatcactcgc cattatgttt ggtggcttgg tacaggctat attgttaatc attgccttag
     4501 ttggcagtca acgaaccgcc cgaagtattt tggggatgta ggaggaaggg agtcattatc
     4561 tcgcctcctc tttttatagc ttacttagat tggagaacat ttatgaacac tcgtgtcttt
     4621 aaggacatct caaaggttca acacagggca tggctaggat ttacaactag acaggttatt
     4681 tttgtatttc cagctgtcgc cctaaccatt ttggtactgg gcttgaacct attttactgg
     4741 caatttgggg attggtttgt ctatggtttt gtttttagct ttaccattcc actcatgtta
     4801 ttcggggtct atcgccccaa cgatttacca tttgaaacct acctcaagta ccgttttcat
     4861 tatgaactaa cagtaccaga tcgtacattt actggaagaa aaggagacca acgtgaaaaa
     4921 attaaaacac tcaatgaaac caaagacctc ttctaatgac aaaaatcaaa agacgaaaat
     4981 acagaagcag gagatcagac cttctaccgt aaatacgtta gcctatcaag ggctttttca
     5041 gaatggcctt atgcaggtca gtccaagcta tttttcacaa acctatcttt taggagatgt
     5101 taattatcaa acagttggct tagatgataa gggagctatc gtggagaaat attccgattt
     5161 aatcaattca ctggatgatc agaccaattt ccaactgact attttcaatc aaaaagttaa
     5221 tttggaaaaa ttccgcaaga gtattctcta ttccttacag aaagatgggt ttgatgctta
     5281 tcgtgatgaa ttgaatcgta tgatggatgc caacttagag gcgggggaga acaacttttc
     5341 agccatcaag tttctttcat ttggcaagag cgaccaaaca ccaaaactag cttttcgttc
     5401 tctgtcacaa attggagaat atttcaagag tggcttttca gagattgatg tatctcttgg
     5461 tcttcttggt ggagaggagc gagtgaatgt ccttgcggat atgttacgag gtgaaaatca
     5521 tttaccgttt tcttacaagg atttgaccct ctcaggtcaa tccaccaaac actttattgc
     5581 cccaacctac ctttccttta aacacaagaa tcatattgaa ttggacgaac gattattaca
     5641 gattgtttat gttcgtgact atggcatgga gttaggggat aaatttattc gagacctcat
     5701 gcagtcagat ttggaagtga tgattagcct acatgctaaa ggctctacca agtctgaaac
     5761 catgaccaag ctgcgaacta agaaaacctt gatggaatca caaaagattg gggaacaaca
     5821 aaagatggct cggactggaa tctatttgga gaaggtcggg catgttcttg aaaacaatat
     5881 caatgaagct gaggctcttc ttcaaaccat gacccaaaca ggagataaac tgtttgacac
     5941 cgtatttcta attggcgtgc tggcggatac tgaagaccaa ctcaaacaat ctcttgatat
     6001 tatcaagcaa gtggcagggt ctaatgatat gattattgat aatttgacct atatgcaaga
     6061 agctgctttt aatagtctct tgccatttgg gaagaactat ctcgaaggtg tttctcggtc
     6121 tctattgact tcaaatattg ccgtgaatgc accttggact tccgtagata ttcaggacaa
     6181 gggtggaaaa ttttatggca tcaatcaaat ctcaagtaat atcatcagta ttgaccgagg
     6241 caagttaaat actccgtcag gtttgatttt agggacttct ggagcgggga aagggatggc
     6301 gacaaaacat gaaatcatct ctactaagct caaggaatca gatagtgata ctgaaattat
     6361 tattgttgac ccagaaaacg aggtgcgaca agaagtcgtg ttataaattg ctagtttagc
     6421 tactctatcc atttggagta atcctaccac agcctgcttg actggtaacg gttatgttgg
     6481 gaagcctgtg ggacaacatg gaaagtctga tagcctaaaa tcggatggac tgttaggata
     6541 agaatactat ggtgaacaga cgtgaaccat cgcaagagtc gttacaggga ctaagtgaaa
     6601 taaggctgat acacttacac caaaaaggta cggaggaggt cattggggct ttcataacct
     6661 gatgacaaat ggacacattc ttcccggctc aaagaaggca cctaaggtat tcacaattat
     6721 ttataacacg gaacttggta agccctatgt attcctttta atcgatagaa ggtattgaac
     6781 tcgtaagagg gatagacgat acagagggta gaggaaagga taaaaagcga atgctccaac
     6841 tgtaatggta ggatataggg gttcaaactt tgccctgacc tgaaagggtg cagacttatc
     6901 ctatggtatt tcatgacaaa aatggaggta aacctatgaa tgataaacat tcaacgacag
     6961 caccagctga gaagttatca cacaagcaac tgcttgcgaa acaatggaaa agcattgatt
     7021 ggaagagagc agaacaagaa gtgaataggc tacaaatcag gattgtcaag gctactcaag
     7081 ccaaacacac taacacagtg aagagacttc aatatttact aacccattcg ttctatgcca
     7141 aggcacttgc cgttcgtcga gtaacgacta acaaaggcaa gaaaacagct ggtatagatg
     7201 gtgaactatg gacggcacct gctcaaaaga tggaagctct cctgtcccta acggacaaag
     7261 gctacaaagc cagtccgtta agacgagttt acatcgacaa aaagggaaaa aagaagaaac
     7321 gtccattagg gattccgact atgtatgaca gagccatgca ggcactctat gctttagcac
     7381 tagagcctgt cgcagagaca actgcggata ctaaatcatt cggcttccga aaaggaagaa
     7441 gctgtcaaga tgcttgtgaa tatatcttca cagcactatc acgaaaagct tctcctcagt
     7501 ggattttaga aggcgacatc agaggctgtt ttgacaacat cagtcatgac tggctcttag
     7561 agaacattcc gatggataaa tctatcttaa aacaattcct taaagcaggt tttgtgttca
     7621 agggtgagtt attcccaacg gaagacggca cgccacaagg cggtatcatt tcgcctatcc
     7681 ttgccaatat ggcactcgat ggattacaac aagtcttatc agatagattt cataccaatc
     7741 gtttaggaaa aatcgacctt cgattcaaaa atagccataa ggtcaatctt gtccgttatg
     7801 c
//
LOCUS       DATUCG010000113         7525 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.115, whole genome shotgun sequence.
ACCESSION   DATUCG010000113 DATUCG010000000
VERSION     DATUCG010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7525
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.115"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            167..1315
                     /locus_tag="VCA20_001932"
     CDS             167..1315
                     /locus_tag="VCA20_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HEP1835690.1"
                     /translation="MKHVFIIGSRGLPAKYGGFETFVQQLVSHQQSERIHYHVACLSD
                     TSHHQHSTYVSADCFTINPPKLGPARVIAYDMMAITYALKIIKEQQIERPIFYVLGNT
                     IGGFIVPFAKKIHSVGGTLFVNPDGLEWKRAKWAKPVQRYLKFSEKMMVKYADLIIAD
                     NEGIEDYIQSEYSATNTRFIAYGTDLSPTKLTKESEQVRQYFTNWNIKEKEFYLIIGR
                     FVPENNYVTAIKEFMASNTRRDLVIVANHEGSAYFQKLKDETGFISDKRIKFVGTVYD
                     QELLKYLRQECRAYIHGHEVGGTNPSLLEALAQTNENLVLGVDFNRKVALEGARYWSK
                     ESGSLSQLINQIDENAEAMELGEIAKKHMKDEYTWEKIVQEYEELFLQ"
     gene            1312..2244
                     /locus_tag="VCA20_001933"
     CDS             1312..2244
                     /locus_tag="VCA20_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEP1835691.1"
                     /translation="MKVNILMSTYNGQQFLAEQIRSIQEQTYTDWTLFIRDDGSSDRT
                     REIIKDFVEQDQRIRFIDVETDENLGVIKSFHRLVHYDTADFYFFSDQDDVWLPNKLE
                     VSLKKAQAYPTNLPLMVYMDLKVVNQNLEVMAESMVKSQSHHANTELVQELTENTVTG
                     GVAMINHHLAEMWQVTEDILMHDWYLALLASAFGNLVFIDQPGELYRQHSDNVLGART
                     LSKRFKKWIRPHILFAVYWDLIKNSQKQARHLLQMPLSQSNRELIEAFVTIMDEPMLE
                     RFRILRKYGLRKNKTFHTLVFTTLIITKFAYNKE"
     gene            2247..3050
                     /locus_tag="VCA20_001934"
     CDS             2247..3050
                     /locus_tag="VCA20_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019802672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP1835692.1"
                     /translation="MDLFSTKNRILLKELIKTDFKLRYQGSVIGYLWSILKPLMLFAI
                     MYVVFIHFLRLGGDVPHFPVALLLGNVIWSFFSEATNMGMVSIVNRGDLLRKLNFPKE
                     IIVLSSIFGAAINFSINLVVVLIFSIFNGVQFSWTILLAIPLFLELFLLATGIAFILS
                     TFFVRFRDLSQIWEVIMQAGMYATPIIYSLSFVMNQSELAAKLIMLNPMAQIIQDMRY
                     FVIDKANLPVWQVISNKAIVLIPYLIPIIVFVIGVNVFNKHSKKFAEIL"
     gene            3050..4285
                     /locus_tag="VCA20_001935"
     CDS             3050..4285
                     /locus_tag="VCA20_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006532551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1835693.1"
                     /translation="MTEKNIAVKVDHVSKFFKLPTEATQSLRTTMVNRLRGIKGYREH
                     HVLKDVSFEVEKGDFFGILGRNGSGKSTLLKIISQIYVPEKGSVIVDGKLVSFIELGV
                     GFNPELTGRENVYLNGALLGFSQEEIDAMYDDIVDFAELRDFMHQKLKNYSSGMQVRL
                     AFSVAIKAQGDILVLDEVLAVGDEAFQRKCNDYFLERKKSGKTTILVTHDMGAVKKYC
                     NKAVLIENGCVKAFGDPDDVSNQYSLDNATTSIQKASVSKNNVVVPKEPEKSSPIKNL
                     KVSLLSSPQTTPIEKIRFRITYDVLENISTYVAFSLTEIDKHIWIYNDNSLDSLTHGV
                     GKKTIEYSCSLESVNNVTLKLQVAVRNAQHEIVAFATSEETPIIMVTRNDISDEMKSE
                     KIASSGLIRRNGKWVTLDE"
     gene            4278..5576
                     /locus_tag="VCA20_001936"
     CDS             4278..5576
                     /locus_tag="VCA20_001936"
                     /inference="COORDINATES: protein motif:HMM:NF012745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEP1835694.1"
                     /translation="MNNLVSVVITCYNHEEYIRQCIESVFNQSYRNIELIIINDGSSD
                     RSEQIIEDALKENPFKETTYIYQKNQGICATRNRAIELISGDFVLFVDSDNYLESNYI
                     EEMLKTAIEENADIIYTSLIDLNTRDVIIPIQKFNLESYFIGNHIDNCSLIRVSKIEN
                     QRYDLNLNRKKLVDYDFFLGLILYKSAKPAPCDSTFLNYRKLVDSISEHGNVKKYSDA
                     YVYIMLKYSAVQPEFSKKGLATYIDNLSTPQWLQQECLLIYLSEDSSFKNFTVQDLSL
                     NKSGIELIHIPKTCKKFRLDFSELPIIVKFISVKLVGKDTHLLPEFTNGIAHLSGYIF
                     GKNDPQLLYDISSYEEDLQVEVKYELEIETMDIIRSVGQKLDDEAKALKSLKNRQEQT
                     LLQNQELENRLSNLQHEYNNVVTSRRWIIATKLINFFRRK"
     gene            5576..7321
                     /locus_tag="VCA20_001937"
     CDS             5576..7321
                     /locus_tag="VCA20_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002267628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-Rha alpha-1,3-L-rhamnosyltransferase"
                     /protein_id="HEP1835695.1"
                     /translation="MKRLLLYVHYNKYDNLSSHVVYQLEKMRPLFEEVIFISNSKLSK
                     LDQERLETSGLITDFLQRKNSGFDFVAWKDGFQKLGFEHVSSYDSVTIMNDTCYGPIW
                     DIKEVFERFENDLSTDFWGMTNHRSTERFKEHLQSYFVTFKKQVVQSPEFKNFWSNVR
                     EYTIVQDVIDYLESPATNIFVDAGFNYKAVFDTTTEELHHMPLPDFSYYNVAKIIEKR
                     VPFVKVKALEGNPQQTSFVIDELAQISDYPTELIVEHMSNMFTPAAYNLLKYKYLDKN
                     IQPEFLDKKVAIHLHTYYVDLLESFLDIFSTFSFEYDLYLTTDKGEKADQIREILLKR
                     EAIASVHITGNRGRDVLPMLKLKEQLSNYDFIGHFHTKKSLEAEFWAGESWRRELLEM
                     LVKPSDAIVSQLAQNDKLGLVIADIPTYFRYISLDPFYEGALSTDMNVLWERMGMKKE
                     LDFQEFSTYVMSYGTYVWFRYDALKQLFDIDIQDEEVPEEPLPLATILHSIERMLVYI
                     AWGNDYDFLISKNPVTLPPFVDVTLLNKREGIRFNPVAPVDFTYFGGLTGALKYFFFI
                     NKSIAKYVVKRLLRR"
BASE COUNT         2496 a         1153 c         1464 g         2412 t
ORIGIN      
        1 aaatggtatt taacttgaat gtgaaatata agtaattgaa gtatatatca aagcaatata
       61 aaatgaatag aacattctgg ttttcgtcag caaactagga tagaattagg aaaaaaaact
      121 aaaaaatggt ataatgaaaa gaactgcaat ttgatggaga ggattcatga aacacgtttt
      181 tattatcggt agtcgtggac tgccagcaaa atatggtggc tttgagacct ttgtccaaca
      241 attggtcagt caccaacaat ctgaaaggat tcattaccat gtggcgtgct tgtctgatac
      301 aagtcatcac caacattcga cctacgtgag tgcagattgc ttcactatta atccaccaaa
      361 acttggacca gctcgtgtga ttgcttatga tatgatggca atcacttatg cccttaaaat
      421 cattaaggaa cagcagatag agcgaccgat tttctatgtc ttgggaaata ccataggtgg
      481 gttcatagta ccttttgcga aaaagattca ctctgttgga ggtaccttgt ttgtcaatcc
      541 agatggtttg gaatggaaac gggccaagtg ggctaagcca gtccaaaggt atctaaaatt
      601 ttctgaaaaa atgatggtca aatatgctga tttgattatt gctgataatg agggaattga
      661 ggattatatt caatctgaat attcggctac caatacacgc tttattgcct atggtacaga
      721 tttaagtccg actaagttaa caaaagaatc tgaacaagtt cgccaatatt ttacaaattg
      781 gaacattaaa gagaaagaat tttatctgat tatcggtcgt tttgtacctg agaataacta
      841 tgtgacagca attaaggaat ttatggcaag caacacgaga cgtgatttgg tcatcgttgc
      901 aaaccatgaa ggctctgcct attttcaaaa attgaaagat gagacaggct ttatatccga
      961 taagcggatt aagttcgtag gaacagtata tgaccaagaa ctacttaagt atctgcgcca
     1021 agaatgtcga gcttatattc atggacatga agttggagga accaatccta gtctcctaga
     1081 agcgctggcg caaaccaatg aaaatttggt tttaggtgtt gattttaacc gcaaagttgc
     1141 cctagaaggt gctcggtatt ggagtaagga gtcaggtagc ctgagtcaat taattaacca
     1201 aattgatgag aatgctgaag cgatggaatt aggagaaatt gcgaaaaaac acatgaagga
     1261 tgaatacacc tgggaaaaaa tcgtccaaga gtacgaggaa ttatttttac aatgaaagta
     1321 aacatactca tgtcgaccta caatggtcaa cagttcttag ctgagcaaat tcgctctatt
     1381 caagaacaga cttacacaga ctggacactg ttcattcgtg atgatggttc cagtgatcga
     1441 actagagaga ttattaaaga ttttgttgaa caagatcagc gaattcgttt catagatgtt
     1501 gagacagacg aaaatctcgg tgtcatcaaa agttttcata ggctagttca ttatgatact
     1561 gctgatttct attttttcag tgaccaagat gatgtttggc taccaaataa actggaagtt
     1621 agcttgaaaa aagcacaagc ctatccaaca aatttgccat tgatggttta tatggacctc
     1681 aaagttgtca atcaaaatct agaagttatg gcagaaagca tggtaaaatc tcaatctcat
     1741 catgccaata cggaattggt gcaagagttg actgagaata ctgtaactgg cggtgtcgcc
     1801 atgattaatc atcatcttgc tgaaatgtgg caagtaactg aggatatttt gatgcacgat
     1861 tggtacttag ccttacttgc ttcagccttt ggaaatttgg tgtttattga tcaaccaggg
     1921 gaactgtacc gccagcattc tgataatgtt ttaggggctc gaacactctc aaaacgcttt
     1981 aaaaaatgga ttcgccctca tattttattt gctgtatatt gggatttgat aaaaaatagt
     2041 caaaaacaag cccgacactt actacaaatg cctctatctc agtcaaatag agaattaata
     2101 gaagcctttg taaccattat ggatgaacca atgcttgaac gattcaggat tttgagaaaa
     2161 tatggtttaa gaaaaaacaa aacctttcat acattggtat ttacaacact cattattaca
     2221 aaatttgcct acaacaagga gtaaatatgg atttattttc cacaaaaaat cgaattcttt
     2281 tgaaagaatt gattaaaact gattttaaac tcaggtatca ggggtcagtc attggctacc
     2341 tttggtctat tttaaaacca ctcatgttat ttgcaattat gtatgtggtg ttcattcatt
     2401 tcttacgctt aggtggagat gtaccacact ttccagtcgc actattacta ggaaatgtca
     2461 tttggtcatt tttctcagaa gcgacaaata tgggcatggt ttccattgta aatcgtggtg
     2521 acttgcttcg aaagttaaat tttcctaaag aaattattgt tttgtcatct atttttggag
     2581 cagccattaa tttttctatt aacttagttg ttgtattgat tttttcaatc tttaatggcg
     2641 ttcaattttc atggacaatt cttttggcta ttccattgtt tttagaacta tttcttttag
     2701 ccacgggtat tgcctttatc ttgtcaacat tttttgttag atttcgtgat ttatcacaaa
     2761 tttgggaagt cattatgcag gcaggcatgt atgcaacacc gattatctac tcattatctt
     2821 tcgtgatgaa tcagagtgag ttggctgcca agttgattat gctaaaccca atggcacaga
     2881 ttattcaaga catgcgttat ttcgttattg ataaggccaa ccttccagta tggcaggtca
     2941 ttagtaataa ggcaattgtt ttgattcctt atttgattcc gattatcgtt tttgtgattg
     3001 gtgtcaatgt ttttaacaag cattctaaga aatttgcgga gattctctaa tgacagaaaa
     3061 gaatattgca gtaaaagtag atcatgtcag caagtttttt aagttaccaa ctgaggcaac
     3121 acagagcttg cgaacaacaa tggtcaatcg gctgagaggt ataaaaggat atagggagca
     3181 tcatgtccta aaagatgtct cttttgaagt ggaaaagggt gatttttttg gtattcttgg
     3241 tcgaaatggg tcggggaaat cgacactttt gaagatcatt tctcaaatct atgtccctga
     3301 aaaaggttct gtcattgtcg atggaaaact ggtttccttc attgagttgg gagttggttt
     3361 taaccctgaa ttgacgggtc gtgaaaatgt atatctgaat ggtgctcttc ttggtttttc
     3421 tcaggaagaa atcgatgcca tgtatgatga catcgttgat tttgcggaac tccgtgactt
     3481 catgcatcaa aaactaaaaa actactctag tggtatgcag gtgcgtctag ccttttcagt
     3541 tgctatcaag gcccaaggtg acatcttggt tctcgacgaa gttttggcag ttggggatga
     3601 agcttttcaa cggaaatgta atgactattt cttggaacgc aagaaaagtg gtaagacaac
     3661 aattctcgta acacatgata tgggagcagt gaagaagtat tgtaataagg cagtactgat
     3721 tgagaatgga tgcgttaagg catttgggga cccggatgat gtatctaatc aatatagttt
     3781 agataatgcg acaacttcca tacaaaaagc ttcagtctca aaaaataatg tagtagtgcc
     3841 taaagaacca gaaaagagtt cacctattaa aaatttaaaa gttagcttat tgagctcccc
     3901 tcagactacg ccaattgaga aaatacgatt tagaattaca tacgatgtgt tggaaaacat
     3961 ttcaacatac gtagcatttt cattgacaga aattgataaa catatttgga tttataatga
     4021 taattcatta gatagtttaa ctcatggagt tggtaaaaaa actattgagt acagttgtag
     4081 tttggaaagc gtcaataatg tcacattgaa attacaagta gcagtaagaa atgctcaaca
     4141 tgaaatagtt gcatttgcaa catcagagga aactcctatt atcatggtga caagaaatga
     4201 tatttctgac gaaatgaagt ctgagaaaat agcatcatct ggactaatta gaagaaatgg
     4261 gaaatgggta acattggatg aataatttag tatctgttgt aattacttgt tataaccatg
     4321 aagaatatat tagacagtgt attgaaagtg tattcaatca gtcatatcgg aatatagaac
     4381 ttatcattat taatgatggc tcatcagatc ggtctgaaca aatcattgaa gatgcactca
     4441 aagaaaatcc ttttaaagaa acgacctata tataccagaa aaatcaaggg atatgcgcaa
     4501 ctagaaaccg tgcgattgaa ttaatcagtg gggattttgt actttttgtt gatagtgata
     4561 actatttaga atcaaactat atcgaagaaa tgttgaaaac agcaattgaa gagaatgctg
     4621 atattattta tacaagtttg atagatctta atactcgcga tgtgattatt cctatccaaa
     4681 aatttaattt agaaagctac ttcattggga atcatattga taattgctca ttaattagag
     4741 tttcgaaaat agaaaatcag cgttatgatc ttaacctaaa cagaaaaaag ttagttgatt
     4801 atgatttttt tctaggattg attttataca aaagtgcaaa gccagccccg tgtgattcca
     4861 cgttccttaa ctacagaaaa ttggtagatt cgattagtga gcatgggaat gtaaaaaaat
     4921 attcagatgc atacgtatat ataatgttga aatattctgc tgtacaacca gaattttcaa
     4981 aaaaagggtt agctacctat atagataatt tatctactcc acagtggtta cagcaagaat
     5041 gtcttttgat atatctatct gaggacagtt catttaaaaa cttcactgtg caagatttat
     5101 cgctgaataa atctggtata gaactaattc atattccaaa gacctgtaag aaatttcgtt
     5161 tagatttttc agaacttcct atcattgtaa aatttatttc agtaaaacta gttggaaaag
     5221 atactcattt actgccagaa tttacgaacg ggattgcaca tttatccgga tacatatttg
     5281 gaaaaaacga tccacaactt ctttatgata tttcttctta tgaagaggac ttgcaggttg
     5341 aagtaaagta tgaattagaa atagaaacaa tggatatcat tagaagcgtt ggccaaaagt
     5401 tagatgatga ggctaaagca ctaaagtctt tgaaaaacag acaggaacaa actttattgc
     5461 aaaatcaaga gctagaaaat agactatcta atttacaaca tgagtacaat aacgtagtta
     5521 catctcgccg atggattatt gcaacgaaac taattaattt ttttaggaga aaatgatgaa
     5581 acgattacta ttgtatgtcc attacaataa gtatgacaat ttaagttccc atgttgttta
     5641 ccaactggaa aagatgcgtc ctttatttga agaagttata ttcatttcaa atagtaaatt
     5701 atcaaagtta gaccaagaac gattagaaac atctggttta attactgatt ttttgcaaag
     5761 aaaaaatagt ggttttgact ttgtcgcatg gaaagatgga tttcaaaagt taggttttga
     5821 gcatgtatcg agctatgatt ctgtgaccat tatgaacgat acttgctatg gtccaatttg
     5881 ggatatcaag gaagtttttg aacgctttga aaatgacttg tctacagatt tttggggcat
     5941 gacaaatcat cgttctacgg aacgttttaa agaacacctc cagagttact ttgtcacttt
     6001 taaaaagcaa gttgttcaat cgccggaatt taaaaacttc tggtcgaatg tacgggagta
     6061 tacaattgta caggatgtta ttgattattt agaatcgcca gcaaccaata tttttgtgga
     6121 tgctggattt aactataagg cagtctttga tacaacaacc gaagagctac atcatatgcc
     6181 tttaccagat ttttcatatt ataatgttgc taagattatt gaaaaaagag taccttttgt
     6241 caaagtaaaa gccttggaag gaaatcccca gcaaacttca tttgtaatcg atgagttggc
     6301 acagatttca gactatccta ccgagcttat tgtcgaacat atgtcaaata tgttcactcc
     6361 ggcagcctat aacttattaa agtacaagta cttagataag aatattcaac ctgaattttt
     6421 ggataagaag gtagcgattc acctacatac ttattatgtt gatcttttgg aaagttttct
     6481 agatattttt tcaaccttta gttttgaata tgatttatat ttgacgactg ataagggaga
     6541 aaaagctgat caaattcgag agattttatt aaaaagagaa gcgattgcct ctgtccacat
     6601 cactggaaac cgtggaagag atgttcttcc aatgctaaaa ttgaaagaac agctatcgaa
     6661 ttatgatttt atcgggcatt ttcatacaaa aaaatcactt gaagctgaat tttgggctgg
     6721 agaatcatgg agaagagagt tgctagaaat gttagtgaaa ccatcggacg cgattgtttc
     6781 ccaacttgca caaaatgata aattgggctt ggttattgca gacattccaa cttatttccg
     6841 ttatatctct ttggatccat tttatgaagg tgccttatct acggatatga atgtgctatg
     6901 ggaaagaatg ggaatgaaga aagagttgga ttttcaggaa ttttctacct atgtcatgag
     6961 ttatggtacc tatgtatggt ttaggtatga tgccctaaaa caattatttg atattgatat
     7021 tcaagacgaa gaagtgccag aagagcctct gcctcttgca acaattcttc attctattga
     7081 gaggatgctg gtgtatattg cttggggcaa tgactatgat ttcctcattt caaaaaatcc
     7141 agttaccttg ccaccttttg tagatgtcac tttattgaat aagagagaag ggattcgctt
     7201 taaccctgtt gcaccagttg attttactta tttcggaggc ttaacaggtg ccttgaaata
     7261 tttcttcttt atcaataagt caattgcaaa atatgtagtg aaacggttgt tgagacgatg
     7321 ataggaaaga ttttagagtt tcttaaagag tatgtgaaaa atattgattt taagaaaagt
     7381 aagtataaac ttttttcaaa tgttccgttt atatttttga ctttaataat tattctttta
     7441 caatggtact atatcggaac tgatattatt aagtctcata gttcggacta tctttttgta
     7501 ctattattgt tattaacaag tttga
//
LOCUS       DATUCG010000114         7505 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.116, whole genome shotgun sequence.
ACCESSION   DATUCG010000114 DATUCG010000000
VERSION     DATUCG010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7505
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.116"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(19..654)
                     /gene="serB"
                     /locus_tag="VCA20_001938"
     CDS             complement(19..654)
                     /gene="serB"
                     /locus_tag="VCA20_001938"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEP1835696.1"
                     /translation="MTTGLLVMDVDSTLILEEGIDLLGEEAGLGAQVAAITERAMRGE
                     LDFEEALRERVALLKGLPVSVFDRIIKKIHFTPGAAELVSELKMRGYKVAVVSGGFHE
                     TVDRLAAQLELDYVRANRLEAVDGVLTGQVLGEIVTKDTKKACLEEWATENGLSLSQT
                     IAMGDGANDLPMIQRAGIGVAFCAKPIVQEQAPYQINEKNLYKLIEILDSK"
     gene            complement(706..2430)
                     /gene="ezrA"
                     /locus_tag="VCA20_001939"
     CDS             complement(706..2430)
                     /gene="ezrA"
                     /locus_tag="VCA20_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEP1835697.1"
                     /translation="MPTGTIILIVSIVIILIIAYVACLIVRKRNDNLLVALEERKEEL
                     FNLPVNEEVEAVKALHLIGQSQVSFREWNQKWVDLSLNSFADIENHIFEAEGYNNAFR
                     FVSAKNAIDSIDSQIDLIEEDIASIRQGLMELKEQEEKNSGRVKHALNLFDSLQEAVR
                     ENPDSYGETLPELEKQLKNIEVEFSEFVMLNSSGDPIEASEILDKTEEHMIALNQIMD
                     RIPSLIERVTKDFPEQLEDLESGYRKLVEQNYLFTEANIESQFQNIRVSIRENTALIV
                     SFDLDAAEAENEGIQAKIDHLYKVFNREIEANKEAVKISKNLPKFLEHVVQNTQLLDE
                     ESQRLNATYLLADSKLSRINQLKARLESIEIVVTESVEDIENPQVAYSILEERLDHSL
                     ASLKEIEEEQLVLADYLKSQELSENTARKKATLYINKLHTLKRYMEKRNLPGIPAEFL
                     TNFFRTSDHVEALIAELDYKRINIEVVNRLLENATYDMNQLEELAYLIVQNATLTEQL
                     LQYSNRYRSFDESVQKAFNRSLSIFEKDFDYQVAFEEISFALETVEPGVTERFVRSYE
                     KTREAIRY"
     gene            complement(2519..4471)
                     /gene="gyrB"
                     /locus_tag="VCA20_001940"
     CDS             complement(2519..4471)
                     /gene="gyrB"
                     /locus_tag="VCA20_001940"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEP1835698.1"
                     /translation="MTEEIKDLQEKAQEYDASQIQVLEGLEAVRMRPGMYIGSTSKEG
                     LHHLVWEIVDNSIDEALAGFADKIEVYIEPDNSITVIDNGRGIPVDIQEKTGRPAVET
                     VFTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVHVYKNGQVYFQEYKRGEVV
                     ADLEVVGETDRSGTTVHFTPDPEIFTETTEFDFAKLNKRIQELAFLNRGLNLSITDKR
                     EGVEQTKEYHYEGGIASYVEYLNENKDVIFETPIYTEGEMDDITVEVAMQYTTSYHEN
                     VVSFANNIHTHEGGTHEQGFRTALTRVINDYAKKNKILKENEDNLTGEDVREGLTAVI
                     SVKHPGPQFEGQTKTKLGNSEVVKITNRLFSDAFAEFLLENPQIARRIVEKGILASKA
                     RIAAKRAREVTRKKSGLEISNLPGKLADCSSNDATKTELFIVEGDSAGGSAKSGRDRE
                     FQAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGADFDVSKARYQKLVIMTD
                     ADVDGAHIRTLLLTLFYRYMRPVVEAGYVYIAQPPIYGIKVGSEIKEYIQPGVNQEQE
                     LQDALERHSTGRSKPTIQRYKGLGEMDDHQLWETTMNPENRLMARVSVDDAAEADKIF
                     DMLMGDKVEPRREFIEENAVYSTLDV"
     gene            complement(4653..5192)
                     /locus_tag="VCA20_001941"
     CDS             complement(4653..5192)
                     /locus_tag="VCA20_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013990578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEP1835699.1"
                     /translation="MTRVAVVLADGFEEIEALAPVDVFRRAHFDCQIVGLDSHEVEGS
                     HGIRVQTDQVFDGDLSSFDLIVLPGGMPGSVHLRDSESLITELQRAVASGKSVAAICA
                     APIVLDKAGLLDSRHYTCFPGKEKDIPSGIHLEEDVVVDGPIITSRGAGTSLDFAYKL
                     VDLFGGDGESLAQTMVYHR"
     gene            complement(5206..6435)
                     /locus_tag="VCA20_001942"
     CDS             complement(5206..6435)
                     /locus_tag="VCA20_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEP1835700.1"
                     /translation="MRKLMKNRGTIDSRVDYSLILPVFFLLVVGIISLYIAVSQDYPD
                     NVVQMVSQQIAWIGIGTVAAFLVMFFSTKFLWQITPYLYVFGLGLMVLPLFFYSPDLV
                     ASTGAKNWVTIGGMTLFQPSEFMKIAYIIMLSRVIVTFHKYYPNRKIREDFMLIGYMT
                     LFTIPVLILLALQKDLGTSLVFVAIFSGMLLLSGVSWKILLPTALTGIVLVGGFMLIF
                     ISPGGTTFLHNLGMDTYKINRIAAWLDPFKNAQSTTYQQAQSLIAIGSGGLKGLGFNK
                     TNLLIPVRESDMIFTVIGEDFGFIGGTVLIVLYLLLIYRMLRVTLKSNNRYYTYISTG
                     YIMMLLFHVFENVGAATGLLPLTGIPLPFISQGGSSMVSNLIGVGLVLSMGYQSRLAD
                     EKETNRSRRYKKITIKR"
     gene            complement(6846..7415)
                     /locus_tag="VCA20_001943"
     CDS             complement(6846..7415)
                     /locus_tag="VCA20_001943"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835701.1"
                     /translation="MILVFYFLLQVMNILFICLVISYRIWNYLLIIPLYLILRFINYE
                     ICRLLGYYALDEMGRFGYGTKEVLYPRFRKIEQVYRKKYNQRSRKHQLIYYAGFVMIY
                     SVSFPFLIIVAMFVTEVVKSMIGENSGEAIIGVFIMSILLLFTLVYGKLQSYKRNYAK
                     WFNLEIIMWEHGHPVFREKKRKRTVFSRA"
BASE COUNT         2294 a         1687 c         1242 g         2282 t
ORIGIN      
        1 gcactcagct acttctcatt atttactatc taaaatctct attagtttat agagattttt
       61 ttcatttatt tggtagggcg cctgttcttg aacaatcggt ttagcacaga aagctactcc
      121 aattcctgct cgttgaatca ttggtagatc atttgctcca tcccccatgg caatcgtttg
      181 agatagactc agcccatttt ctgtcgccca ttcttctaaa catgcttttt tagtatcttt
      241 cgttacaatt tcacccaata cttgtccagt caatactcca tctactgctt ctaatcgatt
      301 agcccgaaca tagtctaact ctaactgtgc agctagtcga tcgactgttt catgaaaccc
      361 gcctgaaact actgctacct tatagcctcg catttttaat tctgagacta attctgctgc
      421 tccaggcgta aagtggattt ttttgataat tcgatcaaac acagaaacag gcaatccctt
      481 taacaacgcc acacgttcac gtaaggcttc ttcaaaatcc aattccccac gcatagcacg
      541 ttcggtaatt gctgcaactt gcgctcctaa accggcctct tctccaagta aatctatccc
      601 ttcttccaaa attaatgttg aatcaacatc cataactaat aatccagttg tcatctccaa
      661 tattcctttc aaagtataaa aataaaaagc caaatggctt tttatctagt agcgaatggc
      721 ttcgcgagtt ttttcgtatg aacgcacaaa acgctctgtt acaccaggtt caactgtttc
      781 cagagcaaat gaaatttctt caaatgctac ttggtaatca aaatcctttt caaaaattga
      841 taatgaacgg ttaaatgctt tttgcacact ttcatcgaat gaacgataac ggttagagta
      901 ttgtagcaac tgctctgtta aagtcgcatt ttgcacaatc aaatacgcca actcttctaa
      961 ttgattcata tcatacgttg cattttccaa caaacggtta acaacttcga tattgattcg
     1021 tttatagtct aactcggcaa tcaaggcttc cacatgatcg ctcgttctaa agaagtttgt
     1081 taaaaattca gctggaattc ctggaaggtt gcgtttttcc atataacgct taagggtatg
     1141 caatttgttg atataaagtg tagctttctt acgcgctgtg ttctctgaca attcttgtga
     1201 tttaagatag tcagctaaaa caagctgctc ctcttcgatt tctttcaagc ttgccaatga
     1261 atgatccaaa cgctcttcta aaatagaata tgcaacttgt ggattttcaa tgtcttccac
     1321 actttctgta acaacaattt caatcgattc caaacgagct ttaagctgat tgatacgtga
     1381 aagtttgcta tctgccagca agtaagttgc atttaggcgt tggctttcct catccaagag
     1441 ctgtgtattt tggacaacat gctccaagaa ttttggcaag tttttactaa tcttaacagc
     1501 ttccttattc gcttcgatct cacgattgaa gactttatac aaatgatcaa tttttgcttg
     1561 aattccctca ttttcagctt ctgcggcatc caaatcaaag gaaacaatca aagcagtatt
     1621 ttcacgaatt gaaacgcgaa tattttggaa ctgtgactca atgttagctt ctgtgaacaa
     1681 ataattctgc tcaaccagtt tacgataacc cgactccaaa tcttccaatt gctctgggaa
     1741 atcttttgta actcgttcaa tcaaacttgg aatgcgatcc atgatttgat tgagggcaat
     1801 catatgctct tcagtcttat caaggatttc agaagcttca atcgggtcac ctgaggaatt
     1861 aagcatcaca aattcagaaa actcaacttc aatatttttc aattgtttct caagttctgg
     1921 tagtgtctca ccgtagctat ctggattttc acgaacagct tcctgtaaac tatcgaacag
     1981 gtttaacgca tgttttacgc gaccagagtt tttctcttct tgctccttca actccatcaa
     2041 accttgacgg atagaggcaa tatcttcttc aattaaatca atttggctgt cgatactgtc
     2101 gatagcgttc tttgcactca caaagcgaaa ggcattgtta tagccttctg cctcaaagat
     2161 atgattttca atatcggcaa atgaattgag cgacaaatca acccattttt gattccattc
     2221 acggaatgac acctgacttt gaccaatcaa atgaagtgct ttgacagctt cgacttcttc
     2281 attaactgga aggttaaata gctcttcctt acgttcttcc aatgcaacca aaaggttgtc
     2341 atttcgttta cgaacaatca ggcaagctac ataggcaatg attaaaataa taacaatcga
     2401 aacgattaag atgattgttc ctgtaggcat gtaaatctcc ttagctcata gtaattacta
     2461 gaaaatatcg cttaattata ccataaatct ctagtatatg cacttgtttt tttagttttt
     2521 agacatccaa tgttgaataa acagcatttt cctcgataaa ctctcgacga ggttcaacct
     2581 tatcccccat tagcatgtca aaaatcttat cggcttctgc cgcatcatca actgaaacgc
     2641 gtgccatgag acgattttca ggattcatcg tagtttccca caactgatga tcatccattt
     2701 ctcccaaacc tttgtaacgt tgaatcgttg gcttggaccg tcccgtacta tgtctttcta
     2761 aggcatcttg caattcttgc tcttgattaa ccccaggttg aatatattct tttatctcac
     2821 ttccaacctt gataccgtaa ataggtggtt gagcgatata tacgtatccc gcttctacaa
     2881 caggacgcat gtagcggtaa aataatgtca gaaggagtgt ccgaatatgg gcgccatcga
     2941 catcggcatc tgtcataatg actaattttt gataacgtgc cttgctaacg tcgaaatcag
     3001 caccaaagcc agttcccata gctgtaaata gactacggat ttcttcgttg gccaaaatct
     3061 tgtccatgct ggctttttct acgttcaaaa tcttaccacg aatcggtaaa atagcctgaa
     3121 attcacggtc gcgaccggat ttagctgaac caccggctga gtccccctca acgatgaaaa
     3181 gctctgtctt cgttgcatca tttgaggaac agtctgctaa cttacctggg aggtttgaaa
     3241 tctcaagacc cgatttctta cgagttacct cacgcgcacg cttagcagca atacgagcct
     3301 tgcttgccaa gatccctttt tcaacaatcc gtctagcaat ctgtggattt tccaaaagaa
     3361 attctgcaaa ggcatcagag aaaaggcggt tggtaatttt gaccacttca ctattcccaa
     3421 gttttgtctt agtttgtccc tcaaattgag gccctgggtg cttaacagaa atgaccgctg
     3481 tcaagccttc acgcacatcc tcaccagtca aattatcctc attttctttg agaattttat
     3541 ttttcttggc gtagtcgttg ataacacgtg tcagagctgt acggaatcct tgctcatggg
     3601 taccaccttc atgtgtgtga atattgttgg caaaactaac tacgttttca tgataactcg
     3661 tcgtatattg catggcaacc tcaacggtaa tatcatccat ttcaccttct gtatagatag
     3721 gggtctcaaa aatgacatcc ttgttctcat taagatactc aacgtaggaa gcaataccac
     3781 cttcgtagtg atattccttt gtttgctcca ctccctcacg tttgtccgta atagagagat
     3841 ttaaaccacg atttaggaag gccaattctt ggatccgctt atttaatttt gcaaagtcga
     3901 actcagtcgt ctcagtaaaa atttctgggt caggtgtaaa atgtacagtt gtaccagaac
     3961 ggtctgtttc tccaacaacc tccaaatctg caacgacttc acctcgttta tactcttgga
     4021 aatagacctg accatttttg tagacatgga cgtctaactg agtagatagg gcgtttacga
     4081 ctgatgaacc tacgccgtgc aatccacctg agaccttata accgcctccg ccaaatttac
     4141 cacctgcatg gagcactgta aagacagttt ctaccgctgg acgaccagtt ttttcctgaa
     4201 tatctaccgg aatcccacgt ccattatcaa taaccgtgat agagttatct ggctcaatat
     4261 aaacttcaat tttatccgca aaaccagcca gtgcctcatc gattgagttg tcgacaattt
     4321 cccaaaccaa gtgatggaga ccttctttag atgttgagcc gatatacatc cctggacgca
     4381 ttcgaacagc ttcaagacct tccaagactt gaatctgact ggcatcatat tcttgagcct
     4441 tctcttgtaa atctttgatt tcttctgtca tttcttttcc ttattttcta tttgttgctc
     4501 cttaaaaata gaaaagccaa tccatctaga taaaagcgag acatattttt agagcaaaaa
     4561 tcattctaac gaataccctt cattatacca tattttctaa tattttgata gaaaaaagaa
     4621 gaggaaacat tagctccatc ttcttcatta ctttaacgat gataaaccat tgtttgagct
     4681 aggctttccc catctccacc aaataaatca accaatttat aggcgaaatc caaacttgtt
     4741 ccagcacctc ggctagtgat aattggacca tcaacgacca catcctcttc cagatgaata
     4801 cctgacggaa tgtctttttc tttccctggg aaacatgtat agtgacgact atctaacaaa
     4861 ccagccttat ccagaacaat tggagccgca cagattgctg ctactgattt tcctgatgca
     4921 accgctcttt gcaattccgt aattagtgac tctgaatctc taaggtgaac agaacctggc
     4981 attcctcctg gtaaaacaat cagatcaaag gaagacaggt ctccatcaaa gacttggtca
     5041 gtttgaacac gaataccgtg cgatccctcc acttcatgac tatctaaacc aacaatctga
     5101 cagtcaaaat gagctcgacg gaatacatct acaggggcca atgcttcgat ttcttcaaaa
     5161 ccatcggcta aaacaaccgc aactcttgtc atataaacca actttctaac gttttatcgt
     5221 aattttttta tagcgacgtg atcgatttgt ttccttctca tcagctagtc gtgactgata
     5281 ccccattgac agcactagac caacaccaat caaatttgaa accatggacg agccaccttg
     5341 ggagataaac ggcaaaggaa tacctgttaa aggcaacaaa cccgttgcag cgccaacatt
     5401 ttcaaacaca tggaacaaga gcatcatgat atagccagtt gatatgtagg tgtagtatcg
     5461 attgtttgac ttcaacgtta cccgcagcat tcgataaatg agcaacaagt atagtacaat
     5521 caataccgtc ccaccaataa aaccaaagtc ctctccaatc accgtaaaaa tcatatcact
     5581 ttctcgaaca ggtataagaa gatttgtttt attgaaacct agtcctttta ggccaccact
     5641 tccgatagct atcaaacttt gagcttgctg gtaggtcgtt gattgggcat ttttaaaggg
     5701 atcaagccag gctgcaatac gattgatttt ataagtatcc atccccaaat tgtggaggaa
     5761 tgttgttcca ccaggagaaa taaaaatcag cataaatcca ccaaccaaaa cgatccccgt
     5821 taaggcagtc ggtaatagaa ttttccaaga aactcctgat aatagaagca ttccgctaaa
     5881 gattgctaca aatactaagg aggttcctaa atctttctgt aaggccaaca aaatcaggac
     5941 agggatagta aacagtgtca tataaccaat caacataaaa tcctcacgaa tttttcgatt
     6001 tggataatat ttatgaaagg tgacaatcac ccttgacaac ataatgatgt aggctatttt
     6061 cataaattcg gatggttgga aaagagtcat tccaccaatc gttacccagt tttttgctcc
     6121 cgttgacgct actaagtctg gactgtagaa aaacaaaggc aataccataa gtcctagccc
     6181 aaatacgtac aagtaaggtg tgatttgcca cagaaactta gtggaaaaga acatgaccaa
     6241 aaaagctgcc actgttccaa ttcctatcca agcaatttgt tgactaacca tctgtacaac
     6301 attatccgga taatcttgac taaccgcaat gtagagagaa ataatcccca caactaataa
     6361 aaaaaagacc ggtaaaataa gtgagtagtc tactcgacta tcgattgttc ccctattttt
     6421 catcaatttc ctcatacttt cacttcaaat ttttactcgt tccattatag caaaaatcat
     6481 ctctcattga aactaaaaat ctagttacat cagtggggat acgatttata ctcaacgaaa
     6541 atcaaaagta gcctaggaaa cgaagtcgaa gatagaactg gagttcatca aggcaagttg
     6601 acaacggata attttgattt tcgaagagta ttactagtga acaaatcata aaatacgtat
     6661 atcttcatac cttcgaacag gctgaaatat aatgctttac cttcacacac cccataagtt
     6721 tgctcaattc atgagtatgt aaaccaatgg caatcatttt ggcaatcacg cacgaaaaaa
     6781 gccctctctt atttctagag ttaaagaaaa aatggtccac tggaccataa ctcgctcttt
     6841 tattttcaag ctcgtgaaaa aacagtccgc tttcgcttct tttctctaaa gactggatgt
     6901 ccatgttccc acatgataat ctctaaatta aaccacttag cataatttct tttataactt
     6961 tgtagcttgc catacactag agtaaataaa agaagaatgc tcatgataaa tactcctatg
     7021 attgcttctc ctgaattctc accaatcatt gatttgacca cctcggtaac aaacattgcg
     7081 acgataatca gaaaaggaaa actaaccgaa taaatcataa caaaacccgc gtagtaaatt
     7141 agctggtgct ttctgcttcg ctgattgtat ttcttcctat agacttgctc tatctttcta
     7201 aaacgtggat agaggacttc cttggtaccg tatccaaagc gccccatttc atccaaggca
     7261 taatatccta acaggcggca aatttcataa tttatgaacc gtaagatgag atagagagga
     7321 ataattaata gataattcca tatccgatag gatattacta gacagataaa cagtatgttc
     7381 atgacttgca aaagaaaata aaaaactaaa atcatgtttt catagtcaac tgaaacaaga
     7441 gtaggataaa gtacctcaag aaaagtatcg caatttggca atactcttct gaggtgtttt
     7501 ttgat
//
LOCUS       DATUCG010000115         7482 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.117, whole genome shotgun sequence.
ACCESSION   DATUCG010000115 DATUCG010000000
VERSION     DATUCG010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7482
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.117"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            35..1408
                     /locus_tag="VCA20_001944"
     CDS             35..1408
                     /locus_tag="VCA20_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017769517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEP1835702.1"
                     /translation="MENHMSYKGTNKLLVGIVLAVLSFWLFAQSILNMAPDVQADLGV
                     PSSVMETGISLTALFSGLFIVVAGGLADKLGRVRFTYFGLLLNIVGSFCLIFSSGAIL
                     FILGRICQGLAAAFIMPATMSLVKTYYDGKERQRAVSFWSIGSWGGSGLCSFLGGAIA
                     STLGWRYVFVFSILASLMSFICLWGTPESKVETKKSRFDLVGLVLFIVSMLALNIGIA
                     SAQERGLFSVVTIGLFLLMLVGLCLFYKVEHSKENAFIDFQLFQNRKYLGATLSNFLL
                     NAVAGTLIVVNTYMQLGRGLSAQVAGELSLGYLACILVTIRIGERLLQQFGARKPMVM
                     GTIMTFIGISLMMFVNISGPMYLVMVFLGYAIYGIGLGIYATPSTDAAIATIPNEKVG
                     SASGIYKMASSLGGAIGVAISIALFYAFSGKNHYDIGLLWGLAVNLVFCILAFVSIMF
                     VMPRKNN"
     gene            1620..2849
                     /locus_tag="VCA20_001945"
     CDS             1620..2849
                     /locus_tag="VCA20_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017769518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HEP1835703.1"
                     /translation="MDKQTYINMLQDVIQIKSENGNEEAVAKYYQNILKNHGIDSKLI
                     PYSEGRSSLVAEITNGEGRVLALSGHMDVVSSGDESQWTHPPFSGDIEDGLIWGRGAS
                     DMKSGLTALVIAFINLKESGQFKGTIRLLATVGEEVGELGSAQLTNLGYLDDVDAVLI
                     GEPCNIGVVHAHKGSLNYKVVSRGVAAHSSTPDLGENAIDHLLTAMTMISERITQQAE
                     EIENPVLGKTFHNITLLSGGSQINSIPDQAMFEANVRTIPEYDNAIVMATVEAVLDEL
                     NQTKGVDLEAVITANQPPVEAAPDSQLVLTILQVANSHDSLKPQFLIQQMNDVLGKNM
                     LSEDMSSSNLDSVKPMVVSGTTDAAQFVRANANLEVAVYGPGMPSLNHKIDERLPLSQ
                     YLDFIEVYQEVIHQYLQ"
     gene            2898..4115
                     /locus_tag="VCA20_001946"
     CDS             2898..4115
                     /locus_tag="VCA20_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006740329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEP1835704.1"
                     /translation="MSIQNILSSIETIKDWQEETYKYLHSHPELSMQETHTAQFIYDT
                     LVSYGYETQKIGGGVVGVLKNGEGKKVLFRADIDALPVKEETGLPYASTVTMKDLDGN
                     LVPVMHACGHDVHIVAGLGAAWAMAQHQTEWTGTYIALFQPGEEIAAGAKSMIEDGLF
                     SKIPKPDLALAQHVLTEPCSGHVGVKEEAFLSSAASVKVTVYGKGAHGSMPHLSIDPV
                     VIASSIVLKLQTIVSREIDPFQFSVVSVGSFQSGLKANIIPDTATLLLNIRTYDEGIQ
                     EHIINAIKRIVTAECMAGGCEKDPEVEVYDTYPLTDNDPQISQKVKQAFMTNLGEDRV
                     ATHYHAMTASEDFSYIPRALGIPYLYWGFGGFVEGQEVYANHNPKFAPAIHPTLETGT
                     QAALIAVLTYLGK"
     gene            4165..5421
                     /locus_tag="VCA20_001947"
     CDS             4165..5421
                     /locus_tag="VCA20_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017769520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEP1835705.1"
                     /translation="MKQKYINANIYGHDKATDFIVENGRFSAIGTDLGHADEIIDVKG
                     KLILPPYVDPHLHLDYYFTGQTDKAENKTGTLFEAIDIWNDFKKGTTKEEMKARMRQA
                     IKDVMSYGTQYIRAQTDCTDPNLTGIKAALELREELKDKVTIQVVAFPQNGMYSYEAE
                     GKTGRDLVEEALQLGADCVGGIPHNEWARSIGEDSIKEIVRLALKYDKYIDVHCDETD
                     DTQARFLELLNAEVLKQSYGARTTASHTCSFGSVNDAYAFRMMGLFKKSGIHFISCPT
                     ENAFLQGRQDSYPKRRGLTRVLEFVDQKINVAFAQDSIVDLWYPAGNGNMMNILDNGI
                     HLAQLMRKDDFDKNFDLVTYNGAKLMGLEKEYGLEIGKPANFIVLDAPSVFEAQRNRV
                     ECLASIRNGFYLFKKAERSHEIGLDI"
     gene            5682..6200
                     /locus_tag="VCA20_001948"
     CDS             5682..6200
                     /locus_tag="VCA20_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEP1835706.1"
                     /translation="MRTYEDKEELKAEIEKTFEKYISEFTNIPESLKDKRIAEVDRTP
                     AENLSYQVGWTSLLLKWEGDERKGLQVKTPSDEFKWNQLGELYQWFTDTYAHLSLQEL
                     KAKLNENINSIYAMIDSLSEEELFEPHMREWADEATKTATWEVYKFIHVNTVAPFGTF
                     RTKIRKWKKIVL"
     gene            6347..7375
                     /locus_tag="VCA20_001949"
     CDS             6347..7375
                     /locus_tag="VCA20_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017769523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEP1835707.1"
                     /translation="MQNIFRTKEIEDAQLRVITITIIAFIIMLLGYWSGNFKISSFAT
                     LGLFTLFYYRNIPMYSLVVRLSIIGLTLLLAFLLGSISSFLPWTTAIVVAVVAFFSRI
                     LTRLYDISKPGVLFYAMLTAMGGSMTVSVEKLPLLILFFLMGVAFSIVGGIIIKSFDS
                     RPETPLMLKSLKRRFYNDPIVIIDGVFYSGALFISVYLSQGLKLQNPYWVTLACASIL
                     LGENLRAIRERHLQYTFGAIAGLILSSLIFYFPFNRLQIVILVTLFYALSQYFVVRNY
                     AVANIFTNPLAILLSMIAKGGQLSSLIQNRLLGILIGGFLGLAVAYIMTVALDHYITL
                     IQEKRDEG"
BASE COUNT         2307 a         1210 c         1593 g         2372 t
ORIGIN      
        1 aattattatt aaattaagaa ggagatgtat tatcatggaa aaccatatga gttacaaagg
       61 gaccaataaa ttattggttg gtattgtttt agcggtttta tcgttttggt tatttgcaca
      121 atctatttta aatatggcac ctgatgtaca agcggatttg ggagtgcctt cctcagtgat
      181 ggagacaggg atatcactaa cagctttatt ttcgggttta tttattgtcg ttgctggagg
      241 cttggcggat aaattagggc gggtgagatt tacctatttt ggtcttttat taaacattgt
      301 gggttcgttt tgtctaatct tttcatcagg agccatttta tttatcttag gaagaatttg
      361 tcaaggatta gcagctgctt ttattatgcc cgctacgatg tcattagtga aaacttatta
      421 cgatggcaaa gaaagacaac gagccgttag tttttggtca attggttcat ggggtggttc
      481 agggctctgc tcttttcttg gaggtgctat tgccagcact ttaggttgga gatatgtttt
      541 tgtattttca atccttgctt cattaatgag ctttatctgc ttgtggggga ctccagaaag
      601 caaagtggaa actaagaaaa gtcggtttga tttagtggga ctggttcttt tcattgtaag
      661 tatgcttgct ttaaatatcg gaattgctag tgctcaggaa agaggcctgt tctctgttgt
      721 tacgataggt ttatttttat tgatgcttgt gggcttatgt cttttttata aagtagaaca
      781 ttcaaaagag aatgcattta ttgatttcca gttgtttcaa aatcgcaaat accttggagc
      841 aacactttca aactttttat taaatgcagt agctggaacc cttatcgtgg ttaacaccta
      901 tatgcaacta ggaagaggct tatcagctca agtagcagga gagttatcat taggttactt
      961 ggcttgtatt ttggtgacca ttcgtatcgg tgagagatta ctccaacaat tcggagcaag
     1021 aaaaccgatg gttatgggaa cgattatgac atttataggg atttcattga tgatgttcgt
     1081 caacatatca gggccaatgt atcttgttat ggtatttcta ggctatgcta tttatggtat
     1141 cggactagga atttatgcga caccatctac agatgctgct atcgcaacta tccctaatga
     1201 aaaagttggt tctgcttctg ggatttataa aatggctagc tctctaggtg gtgctatagg
     1261 tgtcgctatt tcaattgctc tattctatgc tttctcaggg aaaaatcatt acgatattgg
     1321 cttgctatgg gggttagctg taaaccttgt tttctgtata ctggcgtttg tctcaattat
     1381 gtttgttatg ccaagaaaaa ataattagta gaagacctaa ctaatagaat agtgatataa
     1441 taaaattatt gagctactat gattacggtc ggatacttta tgctcaagga tcgttgtatt
     1501 ctagtctttg ctagtatttt taggaatctt gtctgattag taaagaactg tctgttgtag
     1561 taaagtattt cgattataaa gatagatgct cagaacaagt gggaggagga ccctatttaa
     1621 tggataaaca aacttatatc aatatgttgc aagatgtcat tcagattaaa tctgaaaatg
     1681 gaaatgaaga agcagttgcc aagtactatc agaatatcct aaaaaaccat ggaattgact
     1741 ccaaattgat tccttatagt gaaggaagaa gtagcttagt tgctgaaata acgaatggag
     1801 aagggagagt attagcccta tctggtcata tggatgtcgt tagttctggt gatgaatccc
     1861 aatggacgca cccgcctttt tcgggagata ttgaggatgg gcttatctgg ggacgtggtg
     1921 cttcggatat gaaatcagga ttgacagctc ttgtaattgc ttttattaat ttgaaagaga
     1981 gcggtcagtt taaagggaca attagactat tggctacggt tggtgaggaa gtgggggaac
     2041 taggctcggc ccagttaacc aatcttggat acttagacga tgttgatgca gtcttgattg
     2101 gagaaccctg caatattggg gttgttcatg cacataaagg ctctctaaat tataaggtag
     2161 tctctagagg tgttgcagcc cactcctcta ctccagattt aggagaaaat gctattgatc
     2221 atctgcttac agcaatgacg atgatctcag aacgcattac tcaacaagca gaggaaatcg
     2281 aaaatcctgt tcttggaaaa acatttcata atatcacctt gctatcagga ggtagtcaaa
     2341 ttaattcgat tcctgatcaa gcaatgtttg aggctaatgt cagaactatt ccagaatatg
     2401 ataatgcgat tgtcatggca acagtagagg ccgtattaga tgaactcaat caaacaaaag
     2461 gcgtggattt ggaggctgtg ataacagcta atcaaccgcc agtagaagca gcaccagact
     2521 cacagttggt tctgaccatt ctccaagtgg cgaatagcca tgattccctc aaacctcagt
     2581 ttctgattca acaaatgaat gatgttcttg gaaagaacat gctttcagaa gatatgtctt
     2641 cttctaatct tgatagtgtg aaaccaatgg ttgtatccgg tacaactgat gccgcccagt
     2701 ttgtacgcgc aaatgccaat ttagaagtgg ctgtttatgg acctggaatg ccgagcttaa
     2761 accataagat tgacgagcga ctcccactat cacaatattt agactttatt gaagtctatc
     2821 aagaagtgat tcatcaatat ctgcaatgag tcatcaccta tagattttta taagatgaat
     2881 aagtaaagga gaaatacatg agtattcaaa acattttatc atctatcgaa accattaaag
     2941 attggcaaga agaaacctat aaatatctac atagtcatcc cgaactatcc atgcaagaaa
     3001 cacacaccgc tcagtttatc tatgatacct tagtgtctta tggttatgaa acgcaaaaaa
     3061 ttggtggagg ggttgttggt gttttaaaaa atggtgaagg taagaaagtc ctctttcgag
     3121 cagatattga tgctcttcct gtcaaggaag aaacgggatt gccctatgct tcgacagtga
     3181 cgatgaagga tttagatgga aatcttgttc ctgtgatgca tgcctgtggt catgatgtcc
     3241 acattgtagc tggattagga gccgcttggg ctatggcaca acatcaaaca gaatggacag
     3301 gaacttacat tgctttgttc caaccaggag aagagattgc agctggagct aagtcgatga
     3361 ttgaggatgg tctcttttcc aaaattccta aaccagatct ggcactagca cagcatgtgt
     3421 taacagaacc gtgttcaggt catgtgggtg tcaaagaaga ggccttttta tccagtgcag
     3481 cttctgttaa agtaaccgtt tatggaaaag gtgcacatgg ttcaatgccc caccttagca
     3541 ttgacccggt agtgattgcc tcttctatcg tcttgaagtt gcaaactatt gtatccagag
     3601 aaatcgatcc cttccaattt tcagtggtgt cggtgggttc tttccagtct ggcttaaaag
     3661 cgaatatcat cccagataca gctaccttgc tgttgaatat tcgtacctat gatgagggca
     3721 ttcaagaaca tattattaat gccattaaac gtatagtaac cgcagaatgt atggctggag
     3781 gttgtgaaaa agatccagaa gtagaggttt atgacaccta tccgttaaca gataacgatc
     3841 cacaaatcag tcaaaaagtt aaacaagctt ttatgacaaa ccttggtgaa gatcgtgttg
     3901 caactcacta ccatgcgatg acagcttctg aagatttttc ttatatccca cgtgctctag
     3961 ggatccctta tctatactgg ggatttggcg gttttgtgga gggacaagaa gtctatgcca
     4021 atcataatcc aaaatttgct cctgctatcc atccaactct tgagacaggt acgcaagcag
     4081 cgttgatagc tgtattaact tatcttggaa agtaattgct ctactctatt cttaacaata
     4141 ataaaaaatg gagattatta atcaatgaaa caaaaatata taaatgctaa tatttatggg
     4201 catgacaaag caacagactt tattgtcgag aacggtcgtt tttcagctat cgggacagac
     4261 ttaggtcatg ctgatgaaat cattgatgtt aaaggaaagc ttatcctacc accctatgtt
     4321 gatcctcact tacacttgga ttattatttt accggtcaga cagataaggc tgaaaacaaa
     4381 actggcactt tattcgaagc tattgatatt tggaatgact ttaaaaaagg cacaaccaaa
     4441 gaggaaatga aagccagaat gcgtcaagct attaaggatg ttatgagtta tggtacgcaa
     4501 tatatccgtg ctcagacgga ctgtacagat cctaatctaa caggaatcaa agcagcactt
     4561 gaattacgag aagaactaaa agacaaagtg acgattcagg ttgtcgcctt tccacaaaat
     4621 gggatgtatt cttatgaagc tgaaggaaaa acaggtcgcg atttagtaga agaagcactt
     4681 cagcttggag cagactgtgt tggtgggatt ccccataatg agtgggcaag aagtattgga
     4741 gaagattcca taaaagaaat cgttcgtttg gctctaaaat atgataaata cattgatgtt
     4801 cattgtgatg aaacggatga tacacaagcg agatttttag aattattaaa tgcagaagtg
     4861 ttgaaacaaa gttacggtgc tcgtacaaca gcgagtcata cttgctcttt tggttcagtt
     4921 aatgatgcct atgcctttcg aatgatggga ctctttaaga agtctggtat tcattttatc
     4981 tcttgtccaa ccgaaaatgc tttcttacaa ggccgtcaag atagctatcc aaaacgtcga
     5041 gggctaactc gagtcttaga gtttgtagac caaaagataa acgttgcatt cgcacaagat
     5101 tcgattgtcg atttatggta tccagctgga aatgggaata tgatgaatat cttggacaat
     5161 ggtattcatt tagctcaact catgcgaaaa gatgattttg ataaaaactt tgatctcgtg
     5221 acctataatg gagcgaagtt aatggggctc gaaaaagaat atgggctaga gattggaaag
     5281 ccagctaatt ttatcgtttt agatgcccca agtgtttttg aggcgcaacg caatcgtgtg
     5341 gagtgtttag cttctatccg caatggtttt tatttgttta aaaaagcaga acgtagtcac
     5401 gagataggat tagatatcta gtcaataact gtttgttaag tatacattat aatattacgg
     5461 agacatcctt tagactggat accttcgttt ctgtattcat aagtatgatt gaatccgaga
     5521 gaccatcgtt ttaaggagag catctatatc tcaagtaaag ctcaaagtag agatatgcaa
     5581 taagtagtga attcaggatt tgtatatcag ttgaagagat aaaggagaaa aaaagatctg
     5641 tttaatttag agtactgatg aatcagaatg aaggtgattg tttgcgaaca tacgaagata
     5701 aagaagaact aaaagctgaa atagagaaaa catttgagaa atatatttca gaatttacta
     5761 atatcccaga aagcctgaaa gataagagaa ttgctgaagt tgatagaact ccagcagaaa
     5821 acctttcata tcaggttgga tggacaagtt tgcttcttaa gtgggaagga gatgaaagaa
     5881 aagggcttca agtaaaaaca ccatcggatg aatttaaatg gaatcaactg ggtgaattat
     5941 atcagtggtt cacggatacc tacgctcatt tatctctgca agagttgaaa gcaaaattaa
     6001 atgaaaatat taattctata tatgcaatga ttgattcgtt gagtgaggaa gaattatttg
     6061 aaccacatat gagagaatgg gctgatgaag cgactaaaac agcaacttgg gaagtgtata
     6121 agtttattca tgtaaatact gttgcaccct ttggaacttt tcgaactaaa atcagaaaat
     6181 ggaaaaaaat agtattataa agtatattgt ttaactttaa aaatttcata ttataatatt
     6241 agacagatgt tcggtaagta atgaattcag gatttttaca gtagttgaag aggttagata
     6301 gacaatctaa cctccctttt aataaagaaa ggagaatgag attaatttgc aaaatatttt
     6361 tagaacaaaa gaaatagagg atgctcaact cagagttatc accatcacca ttattgcttt
     6421 tattataatg ttattgggtt attggagtgg caattttaaa atttcttctt ttgccacttt
     6481 gggattattt acgctcttct attatcgaaa tattccgatg tattctttgg tagtgcgtct
     6541 gtccattatc ggattgaccc tattgttagc cttcttactt ggttctatat cgtctttttt
     6601 gccctggacg actgctatag tcgttgcagt agttgctttt tttagtagaa tattgacgag
     6661 gttatacgac atttcaaaac caggagttct cttttatgct atgttgaccg ctatgggagg
     6721 tagtatgacg gtttcagttg agaaactacc tcttttaatt ctatttttct tgatgggtgt
     6781 cgcatttagt attgtcggcg gaatcatcat taaatctttt gactcacgac ctgaaacccc
     6841 gctaatgctt aagtcattaa aaagacgctt ttataatgat ccaattgtca ttatagatgg
     6901 cgttttctat agtggagcct tatttatttc tgtttatttg agtcaagggt taaagttgca
     6961 aaatccctat tgggtcacat tagcatgtgc ctctatcctg ctaggagaaa acttgagagc
     7021 cattcgtgaa agacacttac aatatacatt tggtgcgata gcaggattaa ttttgtccag
     7081 tttgatcttt tattttcctt ttaatcgatt acagatagta attcttgtta cactctttta
     7141 tgccttatca cagtattttg ttgtcagaaa ttatgccgtc gccaatatat ttacaaatcc
     7201 cttagcaata ttgctttcta tgatagctaa gggaggtcaa ttatctagtc tcattcaaaa
     7261 ccgattacta ggaattctga taggcggctt tcttggttta gcggtggctt atataatgac
     7321 ggtagctttg gaccattata tcaccttaat tcaagagaaa agagatgaag gatagaagtc
     7381 gtattattgt cttgtacagg aaactgttgt catttcaata gttgttaaat caattgctag
     7441 agtaggttaa ttatgctata atgatttaaa agacatgtag ga
//
LOCUS       DATUCG010000116         7416 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.118, whole genome shotgun sequence.
ACCESSION   DATUCG010000116 DATUCG010000000
VERSION     DATUCG010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7416
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.118"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            406..1179
                     /gene="sufC"
                     /locus_tag="VCA20_001950"
     CDS             406..1179
                     /gene="sufC"
                     /locus_tag="VCA20_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEP1835708.1"
                     /translation="MSVLEIKDLHVEIEGKEILKGVNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPSYEVTQGEILFDGVNILELEVDERARMGLFLAMQYPSEIPGITNAEFLR
                     AAMNAGKEDEDKISVRDFITKLDEKMELLNMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFALLDEIDSGLDIDALKVVSKGINAMRGEGFCAMIITHYQRLLNYITPDVVHV
                     MMEGRVVLSGGPELAQRLEKEGYVQVAAELGIDYKEDDI"
     gene            1196..2458
                     /gene="sufD"
                     /locus_tag="VCA20_001951"
     CDS             1196..2458
                     /gene="sufD"
                     /locus_tag="VCA20_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013989976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEP1835709.1"
                     /translation="MTKEKIQEFSALQAEPTWLTDLRLKAFDKIAELDLPVIERVKFH
                     RWNLGDGSLATNDEIGAVPDFTAIGDNPMLVQVGSQTILEQLPVDLVEKGVVFTDFAS
                     AMDSIPDVLEKYIGTAVAYDEHKLAAYNTTYFNATAVLYVPDNVEIDQPVEALFYQDS
                     TSPVAFNKRVLIIAGKNAKLNYLERFESLGDGAATTSANIVVEVIALAGSQIKFAAID
                     RLGKHLDTYLNRRGYLGNDATIDWAIGLLNEGNVVADLDSELKGNGSHANLKVVGLSS
                     GRQVQGVDTRVTNYGHNSVGHILQHGVILESGTLTFNGIGHIVRGTKGADAQQESRVL
                     MLSDKARSDANPILLIDENEVTAGHAASIGQVDPEDMYYLMSRGLDRATAERLVVRGF
                     LGAVITEIPVKEVRDQLIAVIEEKLTKR"
     gene            2462..3685
                     /locus_tag="VCA20_001952"
     CDS             2462..3685
                     /locus_tag="VCA20_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEP1835710.1"
                     /translation="MDLERIRKDFSILDQVVNDEPLVYLDNAATTQKPQQVLDVLTDY
                     YQKDNANVHRGVHTLSERATARYEAARQKVADFIQAKSSKEILFTRGTTTSLNWVAQF
                     AREILQPDQEVIISVQEHHSNIIPWQQACQQIGAKLRYVPLKDGELDVDSLKSMLSEK
                     TKFVSLVHVSNVLGGVVPIGEIAELVHQVGAYLVVDGAQSVPHMAVNVQELDVDFFAF
                     SGHKMVGPTGIGILYGKEELLNLMSPVEFGGEMIDFVYEQSATWKELPWKFEAGTPNI
                     AGAIGLGAAVDYLTEIGMDAIQAHEAELVDYVFPKLQAIPGLTIYGSQDLSKRTGVIA
                     FNLDDLHPHDVATALDYEGVAVRAGHHCAQPLLRYLQVPATVRASFYIYNTKADCDKL
                     VEAIIKTKEFFNGPI"
     gene            3672..4112
                     /locus_tag="VCA20_001953"
     CDS             3672..4112
                     /locus_tag="VCA20_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEP1835711.1"
                     /translation="MALSRLDSLYMAVVSEHSKSPRHRGSLDGVEKLELHNPTCGDVI
                     ELSVKIEKDVITDIAFDGVGCTISTASASMMTEAVLGKEISQIQELAEIFSQMVQGQE
                     DTRQKELGDASLLAGVAKFPQRIKCATLPWNALKKALERSSCHW"
     gene            4391..5806
                     /gene="sufB"
                     /locus_tag="VCA20_001954"
     CDS             4391..5806
                     /gene="sufB"
                     /locus_tag="VCA20_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEP1835712.1"
                     /translation="MSEERIEPTPIDLGEYKFGFHDENVELVASTGKGLNEEVIREMS
                     RIKGEPEWMLEFRLKSYQTFKKMPMQTWGADLSELDFDDIVYYQKPSDKPARSWDDVP
                     DKIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEYDKHGIIFTDTDTALKEQP
                     ELFKKYFGKLVPPSDNKLAALNSAVWSGGTFIYVPKGVKLDIPLQTYFRINNEGTGQF
                     ERTLIIVDEGASVHYVEGCTAPTYSTASLHAAIVEIIAHEGAYMRYTTIQNWSDNVYN
                     LVTKRARAEKNATVEWIDGNLGAKTTMKYPAVYLEGEGARGTMLSIAFANKGQVQDTG
                     AKMIHNAPRTSSSIVSKSIARCGGEVNYRGQVTFAKNSAKSISHIECDTIIMDDISKS
                     DTIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKEL
                     PMEYAVELNRLIAYEMEGSVG"
     gene            complement(6077..7318)
                     /locus_tag="VCA20_001955"
     CDS             complement(6077..7318)
                     /locus_tag="VCA20_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEP1835713.1"
                     /translation="MRKLLLTLTTILAVFLTSTPILAEDFSVAAKHAIAVEVGSGKIL
                     YEQDAETKASIASISKTLSIYLVYEALAKGEITLDTMVDISDYPYSLTSNTELSNVNL
                     DARSYSVRDLLNASLITSSNSAIIALAEKIAGSEAAFVDRMKAKVQEWGITDEKIVNV
                     SGLDNTDLGENIYPGSSPEDVNMFSALDVAIIARRLILDYPQVLDITSLNAYDFGGYT
                     YYSTNQMLSDGTHARGGVDGLKTGTSNSAGSSFLATTQQAGMRIITVVLNATDGLTSP
                     ENRFVATNDLMNYVYSNFSLVTLVKKGEAFEDSKIKVFNGEKETSPVVAAADLTVVQR
                     NQTDNVPTATFTTDIKEIDAPLKAGTLVGKLQLKDQDLIGKGYVSEQASVDMIIPSDM
                     KEASWPFSWWNQFVRYVNEKL"
BASE COUNT         2143 a         1573 c         1725 g         1975 t
ORIGIN      
        1 gaatctttct aaacaagata agagccgtca ggctcttttt ttgatataat atagtggata
       61 tggttaatta aaattgtcag aaaagactat tttatagatt aacactctct gaaaatcatc
      121 attaacaaga aaagaggctg ggctcaagcc ccgcaccact tctcgaagtt agcgtcacca
      181 tctcagagca gtagtggttg attgggttta acagtccagt ggagtgaaac agtctgggaa
      241 tagactgttt cagctcaaca acttaaaact aggacttgtt gacgaactct tctatgaatg
      301 gtcgatttct tccccactgc caaagttgag tcttatcggt gtatctagtt agcttggtca
      361 gttaatgtgt tttttgaaga gggcaaaaat gaaaaggagc acagcatgtc agtattagaa
      421 atcaaagacc ttcatgtgga aattgaaggc aaagaaatct tgaagggtgt gaatttgacc
      481 cttaaaacag gtgaaattgc agccatcatg ggaccgaacg gaacagggaa atccaccctt
      541 tcagcagcca tcatgggcaa tccaagttat gaagttaccc aaggtgagat tctgtttgac
      601 ggtgtcaata ttttagaatt agaagtcgat gaacgtgccc gtatgggact cttcttggct
      661 atgcaatacc cgtcagaaat tccagggatt actaatgcgg aatttcttcg tgccgcaatg
      721 aacgctggta aggaagacga agacaagatt tctgttcgtg atttcattac taagttggat
      781 gaaaagatgg agctccttaa catgaaagag gaaatggcag agcgctacct caacgaaggt
      841 ttctcaggtg gtgagaaaaa acgcaatgag attctgcaat tactcatgtt ggagccgact
      901 tttgcccttc ttgatgagat tgactcagga ttggatattg atgcccttaa agtcgtgtca
      961 aaagggatca atgctatgcg tggtgaaggc ttttgtgcca tgattattac tcactaccaa
     1021 cgcctgctca actacatcac tcctgatgtt gtccatgtca tgatggaagg ccgtgtcgtg
     1081 ctttcaggtg gaccagaatt ggcgcaacgt ttggaaaaag aaggatatgt ccaagtcgca
     1141 gctgagcttg gcattgacta caaagaagat gacatttaag tctttggagg aagctatgac
     1201 caaagaaaag attcaagaat tttcagcctt gcaggcagaa ccgacttggt tgacagacct
     1261 gcgtcttaag gcttttgaca aaatagcaga gctggacttg ccggttatcg agcgggtcaa
     1321 gtttcaccgt tggaatctag gtgacggaag cctagctaca aatgatgaaa tcggggcagt
     1381 tccagatttt acagctatcg gtgacaatcc tatgttggtt caagttggca gccaaacgat
     1441 tctagaacag ttgccggttg accttgtaga gaaaggtgta gtctttactg acttcgcatc
     1501 ggctatggac agcattccag atgtacttga aaagtacatc ggaactgccg tagcctacga
     1561 cgagcacaaa ttagcagcct acaacacaac ctattttaac gcaacagctg tcctctacgt
     1621 accagataat gttgaaattg accagccggt tgaagctctt ttctaccaag acagcaccag
     1681 tccagttgcc tttaacaaac gtgtcctcat catcgctgga aaaaatgcca agcttaatta
     1741 tctagaacgc tttgaaagtc tgggcgacgg cgcagcaacg acttcagcca atattgtcgt
     1801 ggaagtaatc gcccttgctg gtagccaaat caagtttgcg gctattgacc gtcttggcaa
     1861 gcatttggat acctatctca accgtcgcgg ctatctcgga aacgatgcaa cgattgactg
     1921 ggcgattggt ctgctcaacg aaggaaatgt ggttgccgat ttggacagtg aattaaaagg
     1981 aaacggtagc catgccaacc tcaaggttgt cggactgtca tcaggtcgtc aggtccaagg
     2041 tgtggatacg cgcgtgacca actatggaca taattcggtc ggtcacatcc tgcaacatgg
     2101 ggttatctta gaaagtggaa ccttgacttt taatggtatc ggacatattg tccgtggtac
     2161 taaaggtgcg gatgcccagc aggaaagccg tgtcctcatg ctgtctgaca aggctcgctc
     2221 cgatgctaac ccaattctcc ttatcgatga gaatgaagtc acagctggac acgcagcctc
     2281 aatcggtcag gtggatccag aagacatgta ctaccttatg agtcgtggct tggaccgagc
     2341 aactgctgaa cgcttggttg ttcgtggttt ccttggtgca gtcattacag aaattcctgt
     2401 caaggaagtt cgtgaccaac tgattgccgt gattgaagaa aaactaacaa agagataaga
     2461 tatggatttg gaacgcattc gcaaggattt ttcaatccta gaccaagttg ttaacgatga
     2521 gccgctggtc tatttggaca atgcggcgac cactcaaaaa ccgcagcagg tactagatgt
     2581 gctgacggat tattaccaga aggacaatgc caatgtccac cgtggcgttc acaccctttc
     2641 ggaacgggcg acggctcgct atgaagcggc tcggcagaag gtggctgatt ttattcaggc
     2701 caagtctagc aaggaaattc tcttcaccag aggaacgacc accagtctta actgggtggc
     2761 tcagtttgcc agagaaattc tccagccaga ccaggaagtg ataatctcgg tccaagagca
     2821 ccactccaat atcatccctt ggcagcaagc ctgtcagcaa ataggtgcca agctccgcta
     2881 tgttccacta aaagacggcg agttggatgt ggatagcctc aagtccatgc tctccgaaaa
     2941 gaccaagttc gtctccctgg tccacgtatc caatgtccta ggtggcgtgg ttcctattgg
     3001 ggaaatagca gagctggtgc atcaagtcgg tgcctacttg gtggtggacg gagcccagtc
     3061 agttccccac atggctgtca acgtgcaaga attagacgtg gatttctttg ccttttcagg
     3121 tcataaaatg gtaggaccga caggaatcgg cattctctac ggcaaggaag aattgctcaa
     3181 tctcatgtcg ccagttgaat tcggcggtga aatgattgac tttgtctatg agcagtcagc
     3241 tacttggaag gaattgcctt ggaagtttga ggcaggaacg cccaatattg caggtgcgat
     3301 tggtctggga gcagccgttg attacctgac cgaaattggt atggacgcta tccaggccca
     3361 cgaggcagaa ctggttgact atgtctttcc aaaattgcag gctattccag gcttgaccat
     3421 ttatggcagt caggacctgt ccaagcggac gggagttatt gcctttaact tggacgactt
     3481 gcatccacac gatgtggcaa ctgctctgga ctatgaaggt gttgctgtgc gggcaggcca
     3541 ccattgtgcc caaccgcttc tcagatattt gcaggtgcct gctactgtac gagcaagttt
     3601 ttacatttac aataccaagg ctgactgtga taagttggtc gaggcaatca tcaagacaaa
     3661 ggagtttttc aatggcccta tctagattag actctcttta tatggcagtt gtgtctgaac
     3721 actccaagtc gcctcgtcac cgcggaagtt tggacggagt ggaaaagtta gaactccaca
     3781 acccaacctg tggcgatgtg attgaactat cagtcaagat tgaaaaagat gtgattaccg
     3841 atattgcttt tgacggagtt gggtgcacca tttcaaccgc atcagcttct atgatgactg
     3901 aagcagtgct tggtaaagaa atcagtcaga ttcaagagct ggcggaaatc ttctcacaaa
     3961 tggtccaagg ccaagaagac acacgccaga aggaactggg agatgcctcc cttcttgcag
     4021 gcgttgccaa attcccccaa cgtatcaaat gtgccacatt accgtggaat gccctgaaga
     4081 aagcactgga acgaagttcg tgtcattggt agaaattgat ttcgttttgc gaaatatgaa
     4141 tatttagttc caaccaagtt tagtgtcttg aagaaagcac tggaacgaag tgaaggtgct
     4201 tactgagcat caacaaacaa ataaataaaa atgaaaatca tacagaaagg attgttcagt
     4261 ttctgattgg aaatgaaatt gaactcgccc taaaagcttt tgaaaagaga taaatctcct
     4321 cgtgtgtttg cacacgaagt cgattcccta tttttcattt gctttcttaa ggggcttagt
     4381 atcttatatt atgtcagaag aaagaattga accaaccccg attgaccttg gggaatacaa
     4441 attcggtttc catgacgaga acgtggaact agtggcttcg actggtaagg gtttgaacga
     4501 agaagtcatt cgtgaaatgt cccgtatcaa aggcgagcct gaatggatgt tggaattccg
     4561 gttgaaatcc taccaaacct tcaagaaaat gccgatgcag acttggggag ccgacttgtc
     4621 tgagctggat tttgatgata ttgtttacta tcaaaaaccg tcagataagc cagcgcgtag
     4681 ctgggatgat gtaccagaca aaatcaagga aacctttgaa cgcatcggta tcccagaagc
     4741 tgagcgtgct tacctggcag gtgcttctgc ccagtacgaa tcagaagtgg tctaccacaa
     4801 catgaaggaa gagtacgaca agcacggtat tatctttaca gatactgaca cggctctcaa
     4861 ggaacaacca gagctcttca aaaaatactt tggaaaactc gttccgccgt cagacaataa
     4921 actagctgcc ctcaactcag cggtttggtc aggtgggacc ttcatctatg taccaaaagg
     4981 cgttaaattg gatattccac tccaaaccta tttccgaatc aacaatgagg ggacgggtca
     5041 gtttgaacgc acccttatca tcgtagatga aggggcaagt gtccactatg tagaaggctg
     5101 taccgctccg acttattcaa cggccagcct ccacgctgct attgtggaaa tcatcgccca
     5161 tgaaggtgcc tatatgcgtt ataccaccat tcaaaactgg tcagacaatg tctataactt
     5221 ggtaaccaag cgtgcccgtg ctgagaaaaa tgctacagtt gagtggattg acggaaacct
     5281 tggtgctaag acgaccatga aataccctgc ggtttacttg gaaggtgaag gagcacgtgg
     5341 aactatgcta tccattgcct ttgccaataa aggtcaagtc caggataccg gtgccaagat
     5401 gattcacaac gccccacgta cctcatcttc tatcgtatcc aaatctattg cccgttgcgg
     5461 tggagaggtc aactaccgcg gtcaagtgac ttttgctaaa aactcagcca agtcaatcag
     5521 ccacattgaa tgtgatacca ttatcatgga tgatatttcc aaatcagaca ccattccatt
     5581 taatgaaatc cataattcac aagtggccct tgaacacgaa gcaaaagtat caaaaatctc
     5641 agaagaacag ctctactacc tcatgagccg tggcctatcc gaatccgaag ctacagagat
     5701 gattgtcatg ggctttgtcg aaccattcac aaaagaactc ccaatggaat acgcagtcga
     5761 actcaacaga ctgattgcct atgaaatgga ggggtcggtt ggttagattt ttggcaattt
     5821 agcaaacatg agtttgctaa aagccaaaaa atctccaacc gaggttggag ataaggagcg
     5881 acggaagtcg ctcttcttta taaataacat agtctgggag actagattac cctactgaca
     5941 tcgcttcctg taaggaagtg atatgtaata aagagttccg caggaactct caccaaccga
     6001 tgctggagat aaggagtagc ggatgtcact ctgcgataaa aaatagtatg tcgaaaatcg
     6061 ttcgacatac tattttttat aatttttcat tgacatagcg gacaaattga ttccaccaag
     6121 agaatggcca ggatgcttct ttcatatcgc tagggataat catatcgaca ctagcttgct
     6181 cggaaacata accttttcca atgagatctt gatctttcag ttgcagtttg ccaaccaggg
     6241 tccctgcttt gaggggagca tcaatttctt ttatatccgt tgtgaaagtt gcggtgggga
     6301 cattatcagt ttgatttcgt tgaacaacag ttaaatctgc tgccgcaaca acaggacttg
     6361 tttctttttc gccattgaaa acctttatct tactatcttc aaaagcttca ccttttttta
     6421 ctagcgtaac aagcgaaaag ttagaataaa catagttcat taaatcattt gtcgcgacaa
     6481 atcgattttc tggagaggtc agcccatcag tggcattcaa tactactgtg ataatgcgca
     6541 taccagcttg ttgagttgtt gcaagaaaac ttgaacctgc tgagtttgag gtccctgttt
     6601 ttaagccgtc aacccctcca cgagcgtgag taccatcgct tagcatttgg ttggtgctat
     6661 agtaagtgta gccaccaaaa tcgtaggcgt ttagtgaggt aatgtcgagt acctgaggat
     6721 agtcgagtat taatcgtctt gcaatgatag caacatctag ggctgaaaac atatttacat
     6781 cttcaggact ggaacctgga taaatatttt cacccaagtc ggtattgtct agtccggata
     6841 cattgacaat cttttcgtcg gtaattcccc attcttggac ttttgctttc attcgatcca
     6901 cgaatgcagc ttcgctaccg gctatttttt ctgccagagc aataatagca ctattggatg
     6961 atgttattaa agaagcgttt aataggtctc ttactgaata ggaccgtgca tctaggttga
     7021 cgttgcttaa ttctgtatta gaagtgaggc tatagggata atcagaaata tctaccatag
     7081 tatctaaagt gatctcaccc tttgccagtg cttcgtagac caggtaaatg ctcaatgtct
     7141 tactgataga agcgatgctt gctttggttt cggcatcttg ctcatacaag attttgccgc
     7201 tacctacttc aaccgcgata gcatgcttag ctgcaacaga aaaatcttct gccaaaatag
     7261 gtgtggaagt taaaaaaaca gcaaggattg tagtgagtgt tagtaaaagt ttacgcataa
     7321 tcattccttt ttggggtatt ttattctatt atatcacaag aaatgtgaga aaaatttgga
     7381 aaaaacatgt aaaaaatgag aaaatgatga aaaaat
//
LOCUS       DATUCG010000117         6864 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.119, whole genome shotgun sequence.
ACCESSION   DATUCG010000117 DATUCG010000000
VERSION     DATUCG010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6864
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.119"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            20..247
                     /locus_tag="VCA20_001956"
     CDS             20..247
                     /locus_tag="VCA20_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835714.1"
                     /translation="MSVIERLAEKVARQEEKVSRETEKLEHYRDQLQTAMYSTFIKRQ
                     QSSQLSFHEALEQAFGKETTLQPNYRNEDIE"
     gene            244..633
                     /locus_tag="VCA20_001957"
     CDS             244..633
                     /locus_tag="VCA20_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HEP1835715.1"
                     /translation="MSKTWNFDQPLDDVKSTSSHEERAKIAALFHKQDEKPIEEVDYV
                     AAFEQQQKESESKDVQTLASTVKQNQPKKVNITSDYKQHLADTIAQNNNDISACQKQI
                     EELHQLIDEKKIQNKKLQAISVAIDDL"
     gene            689..976
                     /locus_tag="VCA20_001958"
     CDS             689..976
                     /locus_tag="VCA20_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835716.1"
                     /translation="MKFFQKKTQNQDRFKRLIHRLSEMSDSELTKVEKIQDVVFDSSF
                     KSSERVEISRDIITDEEESLDKSIQEAKNKLNMEQLEKNIERFRQGRRESN"
     gene            1369..2064
                     /locus_tag="VCA20_001959"
     CDS             1369..2064
                     /locus_tag="VCA20_001959"
                     /inference="COORDINATES: protein motif:HMM:NF025007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HEP1835717.1"
                     /translation="MDLSKPFIESDTTATWAALNKWDFDFFNNNYGETKVTVSNADFT
                     ELYEVKISDYINYIQNSSKVDEDKLYLRDWSFEEDCPEISNDYVEPDYCEDIFEGFDE
                     RIFPKSKWLYLGAEGTGTGLHQDLLNTHSWNSLLVGEKEWIFYSPTDTDFLYNGQVDA
                     FNMDFEKYPLLSRANPIRIVQKEGDTVFIPSGWWHQVRNNTATIAITKNFVSRDTLEG
                     FLLENLNLKGGER"
     gene            2457..3042
                     /locus_tag="VCA20_001960"
                     /pseudo
     CDS             2457..3042
                     /locus_tag="VCA20_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000018962.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
     gene            3161..4213
                     /locus_tag="VCA20_001961"
     CDS             3161..4213
                     /locus_tag="VCA20_001961"
                     /inference="COORDINATES: protein motif:HMM:NF012703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEP1835718.1"
                     /translation="MKDYYWDVESGIMYPKEWYQENILNKDNSENIIFDDSLNNLEVI
                     IERGLSNLPSVKISALNLFEIDSVILGLPYDLGSMTVPGARFAPEVIRLYSQPFIFNE
                     SNLSISGGRHQLTGLSMFDNHKVIDIGNIGDVKTINLSREETQSLIKMYSTILTDRTK
                     FSVYLGGDHSVTYSIVQGILEIEETISLIIFDAHSDFQEDLDTRESQVLNNNFMNFII
                     NNSNVEEIIYIGYRDVFDIENPKIKLIPPIALYSSVDKLDDFFDFTRKYYISMDVDCI
                     DPFYFGQTGALIPNGLSPRDLQMVLHYICSNLSVVGMDIVEYSPEHSEEDVVYSMYLS
                     NLILHCISDAINKDLN"
     gene            4225..5184
                     /locus_tag="VCA20_001962"
     CDS             4225..5184
                     /locus_tag="VCA20_001962"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835719.1"
                     /translation="MSNILYNEIMKLEHLIDKKIDLDKIEPLRELIEYYYEFGDCKKA
                     FDYSKELDEIAWGNSTLSVEDQVMIRLRLIGLTYDYDLRSNWKTIMELIQSIEKLSNE
                     LSLPNFLIDRAQLYLLEFSLYRRDFRLFNETKLLLEAKENLDKENKAYFHYILSIDAS
                     FSDIDKAFININEAISYSSLHSLSWFQYNIEFARLLAENDRKGAIEKLNHLIQVLNEY
                     SKENERIYFSKDEVLIEIFFALSYYEESFKGKYDALEKAILLSRKTNSKEIYIESLVR
                     RVLLIRNNNRESKYNFKKMFSQVYELCDIFDYEDLVEDLDGIR"
     gene            5171..5728
                     /locus_tag="VCA20_001963"
     CDS             5171..5728
                     /locus_tag="VCA20_001963"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835720.1"
                     /translation="MVLDNECVEKYVDAVLKEIDKKFFEPICKVIHNNNVEFIYSEEK
                     IDSISAVTKVSSGKITVKLFPTAFNGNDDYFIDSYLNLQHVICHEIAHVLDILSEYKI
                     SNQFLYLTGGLYDNNMNYFPKGKGSYQGTTKLGHYNHFEDFADSFGAVVFGDCYDGGK
                     GVEIDTNRKLLVLRIVQEWIKKLEN"
     gene            5704..6684
                     /locus_tag="VCA20_001964"
     CDS             5704..6684
                     /locus_tag="VCA20_001964"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835721.1"
                     /translation="MDKEIRELEVTDYSEFIELCRNFIFQGKVVVDVLDDICLFDYAN
                     IKFEELRESDLLQSVRGLIIDTANNKMLNKSILRFKAIDELIPNIDKIVGIYEKIDGC
                     LCFSYNYKGEVCWTVRRFLRSEIAERVDSIIREKYQELRNYILENEIVLFCEYVGELS
                     KIVVPYKKEEVVLLAAYDLNQDKKLNRKNLEEISQKYNIKLVKQFDVTIYDVLDSLDS
                     LTTQLEGYIIEFEDGEYMKLSSRFYNLLYDLLEYPSYKRFSYCWAKMKLIGRLGEFYS
                     FISSERNIEHLFNELGDIYQSNFFVTSTVNKFLESHNTEELLFERLSEGL"
BASE COUNT         2561 a          818 c         1235 g         2250 t
ORIGIN      
        1 gtgacaagga ggacacccta tgagtgtgat tgaacgtctg gctgaaaaag tagctaggca
       61 agaagaaaag gtctcacgtg agacggagaa attggaacac tatcgtgacc aactacaaac
      121 agctatgtac agtaccttta tcaaacggca acaatctagt cagttgtcat ttcatgaagc
      181 actagagcaa gcctttggta aagaaaccac actacaacca aattacagaa atgaggatat
      241 agaatgagta aaacatggaa ttttgatcag ccactagacg atgtgaaatc aacatcgtcc
      301 catgaagaac gagctaaaat cgcagcactt ttccataaac aggatgaaaa accaattgaa
      361 gaagtagatt atgtggctgc ttttgaacaa caacaaaagg agtcagaatc taaagatgta
      421 caaacgctcg cttcaaccgt taaacaaaat cagccgaaga aggtaaatat cacaagtgac
      481 tataaacaac acttggctga taccattgca caaaacaaca atgatatttc cgcttgtcaa
      541 aaacaaattg aagaacttca tcaattgatt gatgaaaaga aaatccaaaa taaaaagtta
      601 caggctattt cagtagccat tgatgattta taagtcaggt agtcctatgc gggctacctt
      661 tttacaattt aaggagaagg tgaaggtaat gaaattcttt caaaagaaaa cacagaatca
      721 agaccgattt aaacgactga ttcatcgact atcagagatg tcagatagtg aactaactaa
      781 agtagagaaa atccaagatg tggtctttga ttctagtttc aagtctagtg agagagtgga
      841 aatatctagg gacattataa ccgatgagga agaaagtctt gataagtcga tccaagaagc
      901 gaaaaataaa cttaacatgg aacaattgga aaaaaacatt gagagattta gacaaggtag
      961 gcgagagagc aattaaaaag aagaatataa atttgcgata ctggtgacag ttgaaacgtt
     1021 agaaaagagt ttaacaaagt tatttgctaa tattgaaagt cttaaaaaat aagtattgga
     1081 taactgattg aatttaaaaa tgctattatc gaatattttt gcatttgtta tacaaaaaca
     1141 atcaaatttt aagttcagaa aatacaaaaa aaataacatt aaatacgaat tttttaaaaa
     1201 aaaataaaaa aagtagataa aaacgattga ctaaaagatg tttgaatgat aaaattgtgt
     1261 catgtgaaag gagattgtga tatgagtacg taacttgtgt tctaaaaact tgaaatatgt
     1321 tttttcaagc tttgctaagg attagtagat aaaatgagga gagtgttaat ggatttatca
     1381 aaacctttca ttgagagtga tacaactgct acatgggcag cattgaataa atgggatttt
     1441 gactttttta ataataacta tggtgaaacc aaggttactg tttcaaatgc cgattttaca
     1501 gaattatatg aagtaaaaat atcggattat ataaattata ttcaaaattc atctaaagtg
     1561 gatgaagaca agctttattt acgggactgg agttttgagg aagattgccc ggagatttct
     1621 aatgattatg ttgaaccaga ttattgcgaa gatatttttg aaggatttga tgagcgtatt
     1681 tttccaaaat caaagtggtt atatttaggt gctgaaggaa ctgggacagg cttacatcag
     1741 gatttattga atacccactc ttggaattcc ttattggtgg gtgaaaaaga atggatattt
     1801 tattcaccaa ctgatactga ctttttatat aatggtcaag tagatgcatt caatatggat
     1861 tttgaaaaat atcctctttt gtctagagca aatccaatta gaattgtaca aaaagaaggt
     1921 gacacagttt tcattccttc tggttggtgg catcaagttc gtaataatac tgctacaatt
     1981 gcaattacta aaaattttgt aagtagagat acattagaag gttttttgct agagaaccta
     2041 aacttgaagg gaggtgagag ataatgtcaa aggaattgaa ggctactgaa aaagtagaaa
     2101 ttaaaaaaac aattacacct gaagatttac gaaaaaaagt aggattgaca aaaactcata
     2161 ctcgtagaca taccaataca aattaattgt tttttatatt caaaaggaga tgcttaaaat
     2221 tgttttgttt taggcatttt ctttttatct gtcttataga ttaattaata cttgatgaga
     2281 ataataatct aaccaaattc aatccttgtt aatttgactt ttggagattg caatttggat
     2341 aatcagtctt tttcattttt tcaacaatag ttagatactg tcgagattat cactcttcaa
     2401 atagacttaa caaggtcaga atttgaattt ccccaaaata gaaatataca aattgatttc
     2461 cttacggtca attactagaa agaagccgtt tcaatcgtcg ctctaggcaa ttgatttggc
     2521 tagtctaatt aatccgaaaa gctatgagtg acatgcttct atctgataga gtcgccattg
     2581 tagataattt tcttttgcca ctttgccaac ctgtacgcaa ccacagagct accattttta
     2641 aaggattagc cgatattggc tacaagacct ataaatatct ttgattctat ggtttcaagt
     2701 ttatatgctg gtgatcttat ctggttaatt tctgaaccat tagagtggtg taagcaatca
     2761 gtcatcctag gtgatttagg cttcttaaaa agtgaactta aaaaggactt aaaacaagaa
     2821 ggctactttc tatggatgcc acttcgacaa aacttggcaa gggctgaaga acataactat
     2881 tggaaagtga tggtcaggag acgaactatt gaaacccgtt tttctgaact gtatcacctt
     2941 tttgacatcg tacatacatt agcaagagat ctagttggac tacagttacg aattgaacaa
     3001 aatattctgt ctgaaaattt tagttttttt aaaattaatt agtactatag gtaaaatgat
     3061 ttatataaac catgtcatat aaatataaat tatatatcaa ctaataaaca tagatatatc
     3121 tgagagagga gtaaacatta ttaatccatc agtttttaat atgaaagatt attattggga
     3181 tgttgaatcc ggaattatgt atcctaagga gtggtatcaa gaaaatatct tgaataaaga
     3241 taattctgaa aatattattt ttgatgattc gcttaataat ctagaagtaa ttatagaaag
     3301 aggtttgtct aatttgccca gtgtcaaaat ctcggctctc aatttgtttg agatagattc
     3361 agtaattctg gggcttccat atgatttagg tagcatgact gtcccagggg caaggtttgc
     3421 accagaagtt attcgcctat atagtcagcc ctttattttt aacgaatcca atttaagtat
     3481 ttcaggaggg agacatcaac ttacaggatt atcaatgttt gataatcata aggttattga
     3541 tattggaaac attggtgatg ttaaaacgat aaatctatcc agagaagaaa cacaaagttt
     3601 aataaagatg tattcgacaa tacttacaga tagaacaaaa ttttcagttt acttgggagg
     3661 agatcacagt gtgacttatt caattgttca aggtatatta gagattgaag aaacaataag
     3721 cttaattatt tttgatgctc acagtgattt tcaagaagat ttggatacaa gggaaagtca
     3781 agtcttaaat aataatttta tgaacttcat tatcaataat tctaacgttg aagaaattat
     3841 atatattggc tacagggatg tatttgatat tgaaaatcct aaaataaaat tgatacctcc
     3901 gatagcatta tattcttcag ttgacaagtt agatgacttt tttgatttta cgagaaaata
     3961 ttacatttct atggatgttg attgtattga tccattctat tttggtcaaa caggagcatt
     4021 aattcctaac ggtctttctc caagagattt acaaatggtt ttgcactata tatgttcaaa
     4081 tttatctgta gtaggtatgg atattgtaga gtatagtcca gagcatagtg aagaagatgt
     4141 tgtctattca atgtatttat caaatttaat tttacactgc atttctgatg caattaataa
     4201 agatttaaat taaggagaac catcgtgagt aatatattat ataatgaaat aatgaaactg
     4261 gagcatctaa ttgataaaaa aatagattta gataaaattg aacctttaag agaacttatt
     4321 gaatattatt atgagttcgg agattgtaaa aaagcttttg attattcaaa agaacttgat
     4381 gaaattgcat gggggaatag tactttatct gtagaagatc aagttatgat acgcttaagg
     4441 ttaataggac taacatacga ttatgattta cgttctaatt ggaaaactat tatggaattg
     4501 attcaatcta ttgaaaagct atctaatgaa ttgtcacttc ctaatttttt aattgacaga
     4561 gctcaattat atttacttga attctcgtta tatagaagag attttaggct attcaatgaa
     4621 acaaaattat tattagaagc aaaggaaaat ttagataaag aaaataaggc atatttccac
     4681 tacatattat caattgatgc gtcgttttca gatatagata aagcatttat aaatataaat
     4741 gaggccattt catattcaag cttacatagt ctgtcatggt ttcaatataa cattgaattc
     4801 gcacgattat tagcagaaaa tgatagaaaa ggagcgattg aaaaactaaa tcatttgata
     4861 caagttttaa atgaatattc taaggagaat gagagaatct atttttcaaa ggatgaagtg
     4921 ttaattgaaa tattctttgc actttcttac tatgaggagt ccttcaaagg taagtatgat
     4981 gctttagaga aggcaatttt gttatctaga aaaactaact ccaaagaaat atacatagaa
     5041 tcattggtga gacgagttct tttaatacgt aataataata gagaaagtaa atataatttt
     5101 aaaaaaatgt tttcacaagt ttatgaatta tgtgatatat ttgactatga agatttagta
     5161 gaggatttag atggtattag ataacgaatg tgtagaaaaa tatgttgatg ctgtgttgaa
     5221 ggaaatagat aaaaaatttt ttgaaccaat ttgtaaagta attcacaata ataatgtaga
     5281 gttcatttat tctgaggaaa aaattgatag catttctgcg gtgactaagg taagttctgg
     5341 taaaataact gtcaagcttt ttcctacagc atttaatgga aacgatgact attttattga
     5401 ctcttattta aacttacaac atgtaatttg tcatgaaatt gcacatgtat tagatatttt
     5461 aagtgagtac aaaatttcaa atcagttttt atatttaact ggtggattat atgacaataa
     5521 tatgaactat tttccaaaag gtaaaggtag ttaccaaggc actacaaaat tgggtcatta
     5581 taatcacttt gaagattttg ctgatagttt tggtgctgta gtttttggag attgttatga
     5641 tggagggaaa ggagtagaaa tcgatactaa tcgtaagctg ttagtattga gaattgtaca
     5701 agagtggata aagaaattag agaattagaa gttacagatt attctgagtt tatcgagtta
     5761 tgtagaaatt ttatttttca agggaaagtg gtagtagatg ttttagatga tatttgttta
     5821 tttgattatg ctaatataaa attcgaagaa ttacgagagt cagatttgct tcagtcggtt
     5881 agaggactaa taattgatac tgcaaataat aaaatgttaa ataaatctat tcttagattt
     5941 aaagcaattg atgaattaat tccaaatata gataaaatag ttggtattta tgaaaaaatc
     6001 gatggttgtt tatgcttttc ttataattat aaaggtgaag tttgttggac tgttagaagg
     6061 ttcttgagga gcgaaattgc tgaaagagtt gatagcataa taagagaaaa atatcaggag
     6121 ttgagaaatt atattctaga aaatgaaatt gttctttttt gtgaatatgt tggtgaatta
     6181 tctaaaattg tagtgcctta taaaaaagag gaagttgttt tgctagctgc ttatgattta
     6241 aatcaagata aaaaattaaa tagaaaaaat ttagaagaga tatcccaaaa atataatatc
     6301 aagttagtaa aacaatttga tgttacgata tatgatgtct tggactcttt agatagtttg
     6361 acgacccaac tcgaaggata cataatcgag tttgaagatg gcgaatatat gaaattatca
     6421 agtagatttt acaatttatt atatgatttg ttagaatatc caagctataa acgattttcg
     6481 tactgttggg caaaaatgaa attgattggc agattaggtg aattttatag tttcatatca
     6541 tctgagagga atattgagca tctatttaat gaattgggag atatttatca atcaaatttt
     6601 tttgttacct caactgttaa taagtttctg gagagtcata atactgaaga attattgttt
     6661 gaaagattgt cggaaggctt atgaaaattg aagttctaaa aaacattatt gatagatata
     6721 attgttttat taatgtatcc tatgatatat tagttgataa gaattcaaaa aaagttttgt
     6781 tactaagttc gcaaaataag tactacatat taaaaatcta caaagtaaat aataaactca
     6841 tacaatataa aaatgagtta gaag
//
LOCUS       DATUCG010000118         6647 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.120, whole genome shotgun sequence.
ACCESSION   DATUCG010000118 DATUCG010000000
VERSION     DATUCG010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.120"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(110..733)
                     /gene="nth"
                     /locus_tag="VCA20_001965"
     CDS             complement(110..733)
                     /gene="nth"
                     /locus_tag="VCA20_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEP1835722.1"
                     /translation="MVLSKKRARKVIEEIIALYPDAKPSLDFRNHFELVCAVLLSAQT
                     TDAAVNKVTPGLFAAFPTPQAMAAAEVKDIEPYISRLGLYRNKAKFLKDCAQQLMERH
                     NGIVPQTREELEALAGVGRKTANVVLSVGFGIPAFAVDTHVGRICKHHDIVKKSATPL
                     ETEKRVMEVLPPELWLPAHQAMIYFGREVCHPKNPECEKFPQLYEFD"
     gene            831..1694
                     /locus_tag="VCA20_001966"
     CDS             831..1694
                     /locus_tag="VCA20_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEP1835723.1"
                     /translation="MDLQELCRKVIQARPRNSHKGSYGRVLLIGGLYPYGGAIIMAAL
                     ASVNSGAGLVTVATEKDNIPALHSHLPEAMAFSFDDQALLISSLENADLILIGPGLGE
                     NSRAEQLLDFVFEHLSDQQILVLDGSALTLVAKQNRKNFPCKQVILTPHQKEWERLSA
                     IPVADQTSQRNLEALQSFQENTILVAKSSATQILKTQPNQVTPIGTGGPYQATGGMGD
                     TLAGMIAGFAVQFPHVQLYERILVATYLHSYIADQLAQDYYLVKPTDVSRNIQKTMAS
                     ISNTNNRLIND"
     gene            complement(1865..6622)
                     /locus_tag="VCA20_001967"
     CDS             complement(1865..6622)
                     /locus_tag="VCA20_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEP1835724.1"
                     /translation="MKKKETFSLRKYKIGTVSVLLGAVFLFAGAPSVAADELTSLVET
                     KVEATVPDAIVSESASESPVVEELVDTSVEATPTDVTTTDNVEETLGSEALENITNTE
                     VEATQPAVETPAISEKNVEEEEKLSVADETTAITNQEEAKPQNIDSNTIITAPKVWDS
                     GYKGEGTVVAIIDSGLDVDHDVLHISDLSTAKYKSEKEIEAAKEAAGITYGEWFNDKV
                     VFGYNYVDVNTVLKEEDKRSHGMHVTSIATGNPTQPVAGQLMYGVAPEAQVMFMRVFS
                     DLKATTGAALYVKAIEDAVKLGADSINLSLGGANGSVVNMNENVTAAIEAARRAGVSV
                     VIAAGNDGTFGSDHSNPSADYPDYGLVGAPSTARDAISVASYNNTTVGSKVINIIGLE
                     NNADLNYGKSSFDNPEKSPVPFEIGKEYEYVYAGIGQASDFDGLDLTGKLALIKRGTI
                     SFSEKIANATAAGAVGVVIFNSRPGEANVSMQLDDTAIAIPSIFIPLEFGEALAAKPY
                     KIAFNNETDIHPNPKAGLLSDFSSWGLSADGELKPDLAAPGGAIYAAINDNDYANMQG
                     TSMASPHVAGAAVLVKQYLQATYPTKSPQTIEALVKHLLMSTAKAHVNKETTAYTSPR
                     QQGAGIIDTAAAISTGLYLTGEDGYGSITLGNVEDTFSFTVTLHNITNEDKTLNYSTQ
                     LTTDTVQNGLITLAPRLLAEIPGGKVTVQANSSTTVTINVDAASFAEELTGLMKNGYY
                     LEGFVRFTDVADGGDIVSIPYVGFRGEFQNLAVLEEPIYNLIADGKGGFYFEPVTAQP
                     DTVDISHHYTGLVTGSTELIYSTDKRSDFAIKTLGTFKNEAGYFVLELDESGKPHLAI
                     SPNGDDNQDSLAFKGVFLRNYTDLVASVYAADDTERTNPLWESQPQSGNKNFYSGDPK
                     NPKSSIIYPTEWNGTDSEGNALADGKYQYVLTYSSEVPGAAVQTMIFDVIIDRESPVI
                     TTATYDETNFTFNPRPAIEKGESGLYREQVFYLIADASGVTTIPSLLENGDVTVSDNK
                     VFVAQNDDGSFTLPLDLADISKFYYTVEDYAGNISYEKVENLISIGNEKGLVTVNILD
                     KDTNSPVPILFSYSVTDETGKIVAELPRYAGDTSVLKLPFGTYTFDLFLYDTEWSSLA
                     GETKAVVTISEENSTAEVNFYVTLKDKANLLVDIDALLPSGSTIQLVTADGQTIQLPN
                     AKYSKTDYGKFVPVGTYTILPILPEGYEFLEELDVAVLANQSNVKKLTLINKVALKEL
                     IAELAGLEETARYYNASPELQTAYAKALEDANAVYANKHNQAQVDSAVASLVAAREQL
                     NGQATDKEKLIAEVSNYTPTQANFIYYNAENTKQIAYDTAVRSAQLVLNQENVTQAVV
                     NQALADLLAAKANLDGQKTDISALRSAVSVSSVLKATDAKYLNASENVKQAYDQAVEA
                     AKAILADESASQASVEQALAILTSAQAKLDGVATSTNDAKEPANTATDKKDEGTVTPP
                     PIDSEKVDVQAPPVKATGNSGYVPIGQKPNPQPTLPRPVTLQASLSSSNQEKQVTQLP
                     NTGDNDTRYYLVLGVIIGLGTLLVSKRRHKEEV"
BASE COUNT         1855 a         1407 c         1327 g         2058 t
ORIGIN      
        1 tataattctt ttctgagtta ctattcttat aaaatttaaa aaaatgttca gaatttactc
       61 aaacaatttt acataaaaac taaaaaaatc ttcaaaaaca gaagattttt tagtcaaatt
      121 catataattg tgggaatttt tcgcattcag ggttcttagg atggcaaacc tcacgaccaa
      181 aataaatcat agcttgatga gcagggagcc agagttcagg aggtaagact tccatgactc
      241 gtttttctgt ttccaggggt gtagccgatt ttttgacaat atcgtggtgt ttacaaatcc
      301 gtcccacgtg ggtatcaact gcgaaggcgg gaattccgaa accgacactt aaaacaacat
      361 tggccgtttt tcttcccact cctgccaagg cttctaattc ctcacgagtt tgaggaacaa
      421 tgccattatg ccgttccatc aactgctggg cacagtcttt taaaaatttt gccttgtttc
      481 ggtacaaacc caaacgagaa atataaggct caatatcctt cacttctgct gccgccatgg
      541 cttggggcgt agggaaagct gcgaacaaac caggtgttac cttattcacc gcagcatctg
      601 tagtttgagc agataagagc actgcacata ccaactcaaa gtgatttcga aagtccaagc
      661 taggcttagc atcaggatat agagcgatga tttcttcaat aacttttctt gcacgttttt
      721 ttgataatac catgaattca ttatagcatg aaaacttgga tttatctaaa atctggattg
      781 tcataaacct cattcctcgt ggtataataa aaagaaaagg gggaatatgt atggacttac
      841 aagaactctg tagaaaggtt attcaagcaa gaccacgcaa cagccataag ggaagctatg
      901 gtcgagtctt gctgattggt ggactgtatc cctatggtgg agcgattatc atggccgcac
      961 tagcaagcgt caacagtgga gcaggcttgg taactgttgc tactgaaaaa gataacatac
     1021 cagccttaca tagtcatttg cctgaagcta tggcattttc atttgacgat caagccttgc
     1081 tgataagtag cttagaaaat gctgacttga tattgattgg tccaggtttg ggggaaaata
     1141 gcagagctga acagttattg gactttgttt ttgaacattt atctgaccag cagattttag
     1201 ttcttgacgg ctcagccctt accttagtcg caaagcaaaa cagaaagaac ttcccgtgta
     1261 aacaagtcat cctgacacct caccagaaag aatgggaacg gttatcagcc attccggtgg
     1321 ctgaccaaac aagccaaaga aatcttgaag ccttgcaatc ttttcaagaa aatacaatcc
     1381 ttgtagctaa aagttctgct actcaaatcc taaaaaccca gccaaaccaa gtcaccccaa
     1441 taggaactgg tggtccctac caagcaacag gaggtatggg agatactctt gcaggtatga
     1501 ttgcaggatt tgctgtacaa tttccccatg ttcaactata tgaaagaatc cttgtagcaa
     1561 cctacctcca ctcctatatt gctgaccaac tggctcaaga ttattatctt gtaaaaccaa
     1621 ctgatgtctc aagaaacatc caaaagacaa tggcttcaat ttcaaacaca aacaaccgcc
     1681 taatcaatga ttaagcggtc ttttatataa caaactcgtg attaaagcct gacaaatggt
     1741 agaaaatgat actcaatgca aatcaaaagt tgcctaggaa acgaagctga agatagaact
     1801 ggagttcatc gaggcaagtt gacaacggat aattttgatt ttcgaagagt atgactatat
     1861 tttcttagac ttcttcttta tgacgtcgtt tgcttaccaa cagagtccct agcccaataa
     1921 tgacaccaag aacaagatag tatctcgtat cattgtctcc agtatttggt agttgagtta
     1981 cctgtttttc ttgattagag ctagatagac tagcttgcaa agtgactgga cgaggtaaag
     2041 ttggttgagg gtttggcttc tgacctatcg gtacatatcc tgaattccca gtagctttta
     2101 caggaggtgc ttgtacatca actttttctg agtctatagg tggaggcgtt acagtgcctt
     2161 catctttttt gtcagtggca gtatttgctg gctctttggc atcatttgtt gaagtagcaa
     2221 caccatccag ttttgcctga gcgcttgtca gaatggctag agcttgttcg acacttgctt
     2281 ggcttgcaga ttcatcagct agaatcgctt tcgctgcttc aactgcctgg tcataagctt
     2341 gtttcacgtt ttcagatgca ttgagatact tagcatctgt cgcttttaat acggaagaaa
     2401 cggatactgc gctacgaagg gctgaaatat cagtcttttg accatctaag ttggctttcg
     2461 ccgctaacaa gtccgccaac gcttggttga caactgcctg tgttacattc tcttggttca
     2521 atacaagttg tgctgaacga acagctgtat cataggcaat ttgtttggta ttttcagcat
     2581 tgtaataaat aaagtttgcc tgagtcggtg tgtagtttga tacttcagca attagttttt
     2641 ccttatcggt agcctgaccg tttagctgtt ctctcgccgc cacaagactg gcaaccgctg
     2701 aatctacttg tgcctgattg tgtttgttgg catatactgc attggcatct tctaatgctt
     2761 tagcataggc agtttgaagt tctggactag cattgtaata acgcgctgtt tcttcaagtc
     2821 cagcaagttc agcaatcagt tctttcaaag caactttatt aatcaaggtt aatttcttaa
     2881 catttgactg gtttgcaagt actgctacgt ctaattcttc caaaaattca tagccttctg
     2941 ggaggattgg aaggatagtg taggtaccaa ctggtacaaa tttaccataa tcggtcttag
     3001 aatatttagc atttggtagc tgaatagtct ggccatcagc agttaccagt tggatggttg
     3061 aaccagaagg tagtaatgca tcaatatcta ccagcaagtt ggccttatct ttcaaagtca
     3121 catagaaatt cacctcggca gtgctatttt cttccgaaat cgtcacgact gcttttgttt
     3181 cacctgctag gcttgaccat tctgtatcat ataagaataa atcaaaggtg taagtaccaa
     3241 atggtagctt aagaacgcta gtatcgccgg catatcgtgg taattctgca acaatcttgc
     3301 ctgtttcatc ggtgactgag taagagaaaa gtattggtac aggactattt gtatctttat
     3361 caagaatatt gactgttacc aacccttttt cattgccgat actgatcaga ttctctactt
     3421 tttcatagct gatgttacca gcataatcct ctactgtgta gtagaatttt gaaatatctg
     3481 caagatcaag aggcaatgtg aaggagccat cgtcgttttg tgccacaaat accttgttat
     3541 cagaaacggt tacatcacca ttttctaata aggaaggaat agttgtcaca ccgcttgcat
     3601 ccgctataag gtagaatact tgctcgcgat atagaccgga ttctcctttt tcaatggctg
     3661 gacgagggtt aaatgtaaag tttgtttcat cataggtagc tgtggtgata actggtgatt
     3721 ctctatcgat gataacgtcg aaaatcatag tttgtactgc tgcacctgga acttcagatg
     3781 agtaggtcaa aacgtattga tacttaccat ctgctaaagc atttccttcg ctgtctgtcc
     3841 cattccattc agtagggtaa ataatgcttg attttggatt tttaggatca ccactataga
     3901 agttcttatt gcctgactgt ggttgacttt cccaaagtgg atttgttcgc tcagtatcat
     3961 ctgcagcata aacgcttgcg actaaatccg tataatttct caagaagaca cctttgaaag
     4021 cgagcgaatc ttggttgtca tccccatttg gcgagatagc taaatgaggt ttaccagact
     4081 catcaagctc taaaacaaag tatcctgctt catttttaaa tgtacctaga gtcttgatcg
     4141 caaaatcaga tcgtttgtct gtagaataga ttaactccgt acttcctgta acaagacctg
     4201 tgtagtgatg gctgatgtca acagtatctg gttgtgctgt aacaggttca aagtagaagc
     4261 ctcccttacc atcggcaata agattgtaaa tcggctcttc tagaacggct agattttgga
     4321 attccccacg gaaaccaacg tatggaatgc tgacaatatc accgccatcg gctacatctg
     4381 taaatcgaac aaaaccttca agatagtaac cgtttttcat taaacctgtc aattcttctg
     4441 caaagcttgc tgcatcgaca ttaattgtaa cagttgtact tgaattggct tgcacagtca
     4501 ccttaccgcc aggaatctct gctaatagac gcggagccaa ggtgatcaat ccgttttgga
     4561 cagtatccgt tgttaattgc gttgagtagt ttaaagtctt atcttcgttt gtaatgttat
     4621 gaagtgtgac cgtaaagctg aatgtatcct caacatttcc caaggtaatg ctgccataac
     4681 cgtcttcgcc agtcaaatat aaacctgtag aaatagctgc cgcagtatcg atgatacctg
     4741 caccttgttg gcgaggggaa gtgtaggctg ttgtttcttt gttcacatgt gctttagcag
     4801 tagacataag caagtgtttt actaaggctt cgattgtttg aggggactta gtagggtaag
     4861 ttgcctgtaa atattgtttt actagtacgg ctgctcctgc tacgtgtggt gaagccatac
     4921 ttgttccctg catgttggca tagtcattat cattgattgc tgcataaata gcaccacctg
     4981 gagcagctaa gtctggtttt agctcgccat ccgccgatag accccaactt gaaaaatctg
     5041 aaagaagacc tgctttgggg ttaggatgaa tgtctgtttc gttattgaac gcaatcttat
     5101 atggcttagc cgctaaagct tcaccgaatt ccaatggaat gaagatagat ggaattgcga
     5161 tagctgtatc atccagttgc atgctcacat tggcttcacc tgggcggcta ttgaaaataa
     5221 cgacccctac tgcccctgca gctgttgcat tggctatttt ttctgagaaa ctgatagttc
     5281 ctcgtttaat aagcgcaagt tttccagtca aatctaaacc atcaaaatcc gaagcctgac
     5341 cgattcctgc atagacatat tcatattcct tcccaatttc aaatggtacg ggacttttct
     5401 ctggattatc aaacgaactc ttaccgtaat tcaggtcagc attgttttct aagccaatga
     5461 tattgattac tttactacca accgttgtgt tattataaga agcgacagaa attgcatcgc
     5521 gagctgttga aggtgcaccg accaagccat aatctggata atcggctgac ggattagaat
     5581 gatctgaacc aaatgttcca tcattaccag ctgcaataac aacagaaacc cctgcacgac
     5641 gagcagcctc gattgcagca gtcacatttt cattcatgtt aacaacagag ccattagctc
     5701 ctcccaggct gaggttgatg ctatctgcac ctaattttac agcatcttca atcgccttta
     5761 catacaatgc tgcgccagtt gtagctttga ggtctgagaa tacacgcata aacatgactt
     5821 gcgcttcagg agctacacca tacattaatt gtccagcgac tggttgtgtc ggatttcctg
     5881 tggcaatact cgttacgtgc ataccgtgtg agcgtttgtc ttcttctttc aagacagtat
     5941 tcacatcaac atagttataa ccaaatacaa ccttatcgtt aaaccattca ccgtaggtaa
     6001 ttcctgctgc ttctttagct gcttctatct ctttttctga tttatatttt gcggttgaga
     6061 gatctgaaat atgcaataca tcgtgatcaa cgtcaagacc tgaatctatg attgcaacta
     6121 ctgttccttc gcccttataa ccactatccc aaactttagg tgctgtaatg attgtattac
     6181 tatcaatgtt ttgtggtttc gcttcttcct gattagtaat agcagtagtc tcatctgcta
     6241 cggaaagctt ctcctcttct tctacatttt tctctgaaat agctggagtt tctacagctg
     6301 gttgagtcgc ttctacttct gtatttgtga tgttttcaag agcttctgag ccaagtgttt
     6361 cctctacatt atctgtagtg gttacatcag ttggggtagc ctccacagaa gtgtcaacta
     6421 actcctcgac aacgggactt tctgaggctg attcgctgac gattgcgtca ggaacagttg
     6481 cttccacctt agtctctaca aggcttgtca attcatctgc agctaccgat ggtgcacctg
     6541 caaacaaaaa aactgcaccc aaaagaacag acacagttcc aattttatac ttccgaagtg
     6601 agaaagtttc tttctttttc ataacttttc ctcctagttt ttctcct
//
LOCUS       DATUCG010000119         6640 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.121, whole genome shotgun sequence.
ACCESSION   DATUCG010000119 DATUCG010000000
VERSION     DATUCG010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6640
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.121"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            27..164
                     /locus_tag="VCA20_001968"
     CDS             27..164
                     /locus_tag="VCA20_001968"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835725.1"
                     /translation="MEVNNILYFSSDLVAPNAHVSKDKLEAAGFGWVFDCDGVRVVEV
                     E"
     gene            164..514
                     /locus_tag="VCA20_001969"
     CDS             164..514
                     /locus_tag="VCA20_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018165951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEP1835726.1"
                     /translation="MAGRKSISKKMRQQVLSKYNNHCAYCGKVLDLKSLRVDHLHPHY
                     RGGGDHIDNYMPACYQCNFYKSTHTLDDFREKLLSLHERVAQPFIARLGMQYGIVVIK
                     QFDGKFYFEKVEVE"
     gene            769..1203
                     /locus_tag="VCA20_001970"
     CDS             769..1203
                     /locus_tag="VCA20_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEP1835727.1"
                     /translation="MNKAKELLDELQNLDEEIQNRIDELANLEASLLSSPKMNVNKVQ
                     GGQKIQLDERYIDIFSMQDSLKEYMKDATEEAIKRRIELSRLIDKMPKPASRTILRMV
                     YIQKASVYDMIEFLQCSKTTFYKKKKDAIRELGAVIDKSELK"
     gene            1290..1379
                     /locus_tag="VCA20_001971"
     tRNA            1290..1379
                     /locus_tag="VCA20_001971"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1325..1327,aa:Ser,seq:gct)
     gene            1654..2100
                     /locus_tag="VCA20_001972"
     CDS             1654..2100
                     /locus_tag="VCA20_001972"
                     /inference="COORDINATES: protein motif:HMM:NF015550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HEP1835728.1"
                     /translation="MNSRERRFADEYIANGNNATRAYKVVYPTSGDNTARTKGSNWVA
                     KDNILNYIKIKTKERLDASGLKANDIIDRLIDIAFGRPIIGYSKQTNKITGEVIKHIE
                     YENTAPIDEQIKALELLGKYLKLFTDKVEAEVNGTVVFANEADIPD"
     gene            2078..3382
                     /locus_tag="VCA20_001973"
     CDS             2078..3382
                     /locus_tag="VCA20_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HEP1835729.1"
                     /translation="MKQTSQISVDLPKIVGRGYGQFWRSRNFYRVVKGSRGSKKSKTT
                     ALNFVVNLLKCPWANLLVVRRYSNTNKQSTYTDFKWACNQLKVSHKFKFNESLPEITV
                     KATGQKILFRGLDDELKITSITVDVGSLCWAWFEEAYQIETEDKFSTVVESIRGSLQD
                     TSAFAFNRLDGTYGNVPFYKQITVTFNPWNERHWLKKVFFDEDTKRSDTLALTTTFRC
                     NEWLDEVDIKRYEDLYITNPRRARIVCDGEWGVAEGLVYENVQVADFDKESLLQSGNY
                     KLCVGLDFGFTHDPTALVAYLVDDKNKNIYVFDEHYQVGLFTKDIAEMIKNKGYASSQ
                     IVADCAEARLIEELKSEHGIRRIKASRKGKDSIMAGVSKLQGYKIMVHPSCTHIMDEF
                     YSYCYQQDKEGKWLNKPEDKNNHLMDALRYGLQCAERKEWLY"
     gene            3393..4892
                     /locus_tag="VCA20_001974"
     CDS             3393..4892
                     /locus_tag="VCA20_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000894655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEP1835730.1"
                     /translation="MLDVKDISQVERGIKKLIDNDLTDQSKEKMRTALDYYRGKHDIL
                     DYRLYYIDDTGQPKEEKYRSNTKIAHGFFTELVDQKVQYLLSNPIEIQTEQQELQEYL
                     AEYVNEEFQLVLQELVEGASQKAIEYVFWRIDKDGRLVFEVADALKVIPIYNHDNQME
                     QVLYYVKDEIVVDGKTKEVKKIQLWTKDAIHYFVYADNKVKLDDTVPVNPAPHLLVKD
                     KKNEEVLGKGYGRLPFMVLHNNREKLTDLEPIKDLIDDYDLMACALSNNLIDFDHPIY
                     AVRGFEGDSFDKLVTNLKAKKTVGVGPDGGLDVKTVDIPIEARKEKLRIDKEAIYKFG
                     MGFDSSQAGDGNITNVVIKSRYSLLDLKCNKIEVRLRRIIKQMLELIIENINQRYSKA
                     YNTSDIEIIITRDIMANEVDNATIAKTEADTKQVAIQTILDAAPRLDSRTVLELLAGI
                     LEIDPDEVEKALDEEEYQGDLEQDTEVEDGVEPVSEGDATSTTTTGPEG"
     gene            4828..6414
                     /locus_tag="VCA20_001975"
     CDS             4828..6414
                     /locus_tag="VCA20_001975"
                     /inference="COORDINATES: protein motif:HMM:NF019926.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835731.1"
                     /translation="MELNQYQKEMQHLLQLQDQRVNRQLYNLYVDIVKELKKSLLAEY
                     QSYENLSRSKKMDLKRMSAMLEAIDSQSQKLKTGLNGTIPDHLEKVGKMAYNELFYEY
                     EQTRSAIQFALLPEEELKTIIETPVAGLRLSQRINDGVVSDLRKNIKSELTRSFVHGL
                     SYQRTAKRLSELGSSSYRRALNITRTEAGRVSAIARQRSQKEAVDLGIEFKKMWVATL
                     DKRTRNTHQYLDGQQVGPEEYFEHNGLRTLQPHMFGIASEDCNCRCRTISRLKDDDTP
                     LLRRDSETGEVVEYRNYREWEKNRIQVESSIAKVYNQDMFTIDLMDKQRSFTVGSDIR
                     VKTKKLTGIDFDFWAQDNTKKIRDTVFNVQSNLQELKDYTIPTVVFLKKSRLPGFAGY
                     DYKQDILFVSDTLHSEKEFAKVLSDNYFAAQNIKDTMIHELTHKKHWDSAKAFYKAHK
                     KRYNSVEQAMSELNSPLVSYVKEQLKHDFNYLVNISPNADEAFILGDVNELVAEVSVL
                     GERTSDQILYQKVQEVLSWK"
     gene            6405..6593
                     /locus_tag="VCA20_001976"
     CDS             6405..6593
                     /locus_tag="VCA20_001976"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835732.1"
                     /translation="MEVMAMPSKATVMFYNQVRPWIVSGEMKNGIMNYKFADDTPNKV
                     LELFSELKDKLSYPSVAL"
BASE COUNT         2185 a         1107 c         1556 g         1792 t
ORIGIN      
        1 tggagatatt caatgggcaa ccactcgtgg aagtaaataa tattttgtat ttcagttcgg
       61 acttggttgc tccaaatgcc catgtgagta aagataaact tgaagcagct ggctttggtt
      121 gggtgtttga ttgcgacggt gtgagggtcg tggaggtgga gtgatggccg gtagaaaatc
      181 tatctcaaaa aaaatgcgac aacaagtctt atcaaaatac aataatcatt gtgcgtattg
      241 tggaaaggta ctggatttaa agtcattgag agtggaccac ttgcatccgc attatcgagg
      301 cggaggtgac catattgaca attatatgcc agcttgctac caatgcaatt tctataagtc
      361 gacccataca ctagatgatt ttcgtgagaa gttattatct ctacacgaga gggttgccca
      421 accgtttatt gcaagattag ggatgcagta tggcattgta gtgattaagc aatttgatgg
      481 taaattttat ttcgaaaaag tggaggtgga gtgatgaaac cgataaagta cccatattca
      541 ggaaagacta agattgtcag gaaagaaatg ccaaggtttg tcatgctgag ctatacagct
      601 ttcgatagta ggttggttga tcacatatga agaaaaacaa atcagagtgc cgttaccgct
      661 aattaaagtt gtaaagatcc ttaatcagta ctaaaaaaag ccaaggcact ctctgccctg
      721 gctgtggttt aacgctatca atattatacc acaaaaagga gacagagagt gaacaaggct
      781 aaagagttac ttgatgaact gcaaaatttg gatgaagaaa tacagaatcg aattgatgag
      841 cttgctaatc ttgaagctag tttactttct agtcctaaaa tgaacgtgaa taaggttcaa
      901 ggtggtcaga agattcaatt agatgaacgt tacattgata tttttagcat gcaagattcc
      961 ttgaaagagt atatgaagga tgccactgaa gaagctatca aacgcagaat tgaattgagt
     1021 aggctgattg ataaaatgcc aaaacctgca agtcgaacaa ttctaaggat ggtgtatatt
     1081 cagaaagcaa gcgtatatga tatgattgag tttttacaat gcagtaagac tactttctat
     1141 aaaaagaaga aagatgcaat ccgtgaatta ggtgctgtga ttgacaaaag tgaactaaag
     1201 tgaactagtc tgtagcgcac tggtcaaaca atcgtgctat tatagtatca tcaagaaata
     1261 agggtaaggt agtaagtctt gcctgttatg gagagttggc agagttggtc gaatgcgctc
     1321 gtttgctaga cgggtggccg cctacgtgcg gtccgtgggt tcgaatccca cactctcctt
     1381 tgagtgtttg tgtcccagaa tggggtaagt cgttggacga gaattcatat atcactcatc
     1441 aactttgaaa tggttgcgga tgcgactagg ccctgcatgg ttgcacagct acttatatcc
     1501 taggtaagtt ataagctggg cggtttgatt ccgctagggg tctttctcct atatttttcc
     1561 ccacacaatg aagtgtggtt ttttgattgg aggtgatgga aaatcgcaaa gaaaaataaa
     1621 agcaaaagaa gagaacagat tgatgagttt ggtttgaata gccgtgaacg tcgatttgcc
     1681 gatgagtaca tcgcgaacgg aaacaatgct actcgagcat ataaagttgt atatcccaca
     1741 tctggcgata atacagctag aacgaaaggc tctaattggg ttgcaaaaga taacatttta
     1801 aactacatca aaatcaaaac caaagaacgc ctagatgcat caggtctgaa agcaaatgat
     1861 attatcgatc gcctaatcga tattgcattt ggtcgtccaa ttatcggata cagtaagcag
     1921 acgaataaaa taacgggaga agttataaaa catattgaat atgagaatac agcgcctatt
     1981 gacgagcaga taaaagctct ggaattgtta ggcaagtatc tcaagttatt tacagacaaa
     2041 gtcgaagctg aggtaaacgg aacggtggtg tttgctaatg aagcagacat cccagattag
     2101 cgttgatttg cccaagattg ttggcagggg ctatggtcaa ttctggcggt ctaggaattt
     2161 ttacagagtc gtcaaaggca gccgcgggag taaaaagtcg aaaacaaccg ctttgaactt
     2221 cgtagtcaat ctgctgaagt gcccatgggc taatctactt gtcgtgcgtc gctactcgaa
     2281 taccaataag caatcaactt atacggattt taaatgggcc tgtaaccagc ttaaagttag
     2341 tcataagttt aagtttaacg agagtctgcc agagataaca gttaaggcta caggtcagaa
     2401 aatcctcttt cgaggtttgg acgatgagtt gaagattaca tctatcacgg ttgatgtagg
     2461 ctctctttgc tgggcttggt ttgaagaagc ctaccagatt gagactgaag ataaattctc
     2521 gacggtcgtt gaatcaatcc gtggtagctt acaagatact tcggcatttg ctttcaatcg
     2581 actagacggt acatatggaa acgtgccgtt ttacaaacaa atcacggtca cgttcaaccc
     2641 gtggaacgaa cgtcattggc ttaaaaaggt attttttgat gaagatacca aacggtcaga
     2701 tactctggct ttgacaacta cttttagatg caacgaatgg ctagatgagg ttgatattaa
     2761 gcgatatgag gacctttata tcactaatcc acggcgggct cgcattgtct gtgatggtga
     2821 gtggggagtt gccgaaggtt tagtctatga aaatgtccag gttgccgatt ttgacaaaga
     2881 gtcgttattg caatctggga attacaagct gtgcgtcgga cttgactttg gtttcacgca
     2941 cgacccgacc gctcttgtag cttatctcgt agatgataaa aataagaaca tctatgtctt
     3001 cgatgagcat tatcaagtcg gtttatttac taaggatatt gcagagatga taaagaacaa
     3061 aggatatgcc agtagtcaga ttgtcgcaga ttgtgcggaa gctcgactta tcgaagaatt
     3121 gaaatcggag cacggaattc gcaggattaa ggcaagtcgc aaaggtaagg atagtatcat
     3181 ggcaggagtg tcaaaattgc aaggatacaa gataatggtg catccatctt gcacgcatat
     3241 catggatgaa ttttactcat attgctacca gcaagacaag gaaggtaagt ggttgaataa
     3301 gccagaggat aagaataacc atcttatgga cgcactgcga tatggcctgc aatgtgcaga
     3361 gcgcaaagaa tggctctatt agaaagggaa gtatgttaga tgtgaaagat atatcccaag
     3421 ttgaacgagg gataaaaaag ttaatcgaca atgaccttac tgaccaaagc aaagagaaga
     3481 tgcggacagc tttggattac tacagaggta agcatgatat tttggattat agactgtact
     3541 atattgacga tactggtcaa ccaaaagaag aaaaatacag aagtaataca aaaatagctc
     3601 atggtttttt cacggagcta gttgaccaga aagtccagta tttattatca aatcctatcg
     3661 agattcagac ggaacaacaa gagctacagg aatacttagc tgaatatgtc aatgaagaat
     3721 ttcagctagt cttgcaggaa ttggtagaag gggctagcca aaaggctatt gagtatgttt
     3781 tttggcgaat agacaaagat gggcgtttgg tttttgaggt agctgatgcc ttgaaagtga
     3841 ttcctatcta caatcacgac aatcagatgg aacaggtcct gtactatgtt aaagacgaaa
     3901 tagttgtaga tggaaaaacc aaagaagtaa agaaaattca gctttggaca aaagatgcaa
     3961 tccattattt cgtttatgcg gataacaaag tcaaattgga tgatacagtg cccgtaaatc
     4021 ctgcccctca tttattggta aaggacaaga aaaacgaaga ggtgctaggc aaggggtatg
     4081 gtcgtttgcc ttttatggtc ttgcataata atcgcgagaa gttgacagac cttgagccaa
     4141 tcaaagacct aatagacgat tatgacttga tggcttgtgc gctatcaaat aacttgattg
     4201 atttcgacca tcctatctat gccgtaagag gattcgaagg ggatagtttc gataaacttg
     4261 tgaccaattt aaaagcaaag aaaacggtcg gcgtcggtcc tgacggagga cttgacgtaa
     4321 aaaccgtaga catccccatc gaagctcgta aagagaaatt gcggattgac aaagaagcca
     4381 tctataagtt cggcatgggt tttgatagtt cgcaggctgg cgatggtaac atcacgaacg
     4441 ttgttataaa gtcgcgttat agtctgttag acctcaaatg caataaaatt gaagtgcgtt
     4501 tgcgcaggat tattaagcaa atgttagagc tgattattga aaatatcaat cagaggtact
     4561 ctaaagccta caatacctca gatatcgaaa ttattatcac tcgagacata atggcaaacg
     4621 aagtagataa tgcaactata gcaaaaacag aagcagatac gaaacaagtt gcaattcaaa
     4681 ctatcttaga tgctgcgcct cgcttagata gtcgtactgt tttagaacta ttagctggca
     4741 tactcgaaat tgatcctgat gaggttgaaa aagccttaga cgaagaggag tatcaagggg
     4801 atttagagca agatacggag gtagaggatg gagttgaacc agtatcagaa ggagatgcaa
     4861 catctactac aactacagga ccagagggtt aatcgccagt tatacaacct ttacgtggac
     4921 atcgttaagg aattaaaaaa atctctttta gctgagtatc aaagctacga aaatctatcg
     4981 cgtagcaaaa agatggactt gaagcgcatg tcggctatgc tagaagcgat tgacagccag
     5041 tctcagaagc ttaaaacagg tctgaatggt acaataccag accacttgga aaaagtcggt
     5101 aaaatggctt ataatgagct attttacgaa tatgagcaga cgagatcagc aatccaattc
     5161 gctttgttgc cagaagaaga attgaagacg attatagaga ctcctgtagc aggtctgcgc
     5221 ttgtctcaaa gaataaatga tggagttgtg tcggacttac gaaagaacat caaaagcgag
     5281 ctaacacggt cgtttgtgca tggattgagt tatcaaagaa cagctaaaag actatcagag
     5341 ttgggcagtt cgtcttatcg cagagcgttg aatatcactc ggacagaagc tggcagagta
     5401 tcagctatcg ctcgtcagag gtcgcaaaaa gaagcggttg acctaggcat agagttcaaa
     5461 aaaatgtggg tagcaacact ggataaacgg acgcgaaata ctcatcaata cttagacggt
     5521 caacaagttg gtccagagga gtattttgaa cataatggtt tgagaacttt acagccccac
     5581 atgtttggga tagccagcga agattgtaac tgccgctgtc ggactatatc tcgcttaaaa
     5641 gatgacgata caccgctgtt gcgtagggat agcgaaacag gagaggttgt tgagtatcgg
     5701 aattatcggg agtgggagaa aaatcgcatt caggttgaaa gttcgattgc taaagtgtat
     5761 aatcaggata tgtttacaat tgacttaatg gataagcaac gttcctttac agtaggtagt
     5821 gatatacgag tcaaaacaaa gaaattgact ggtatagatt ttgatttttg ggcacaagac
     5881 aacacaaaga aaatcaggga tactgttttc aatgttcagt ccaatcttca ggagttaaag
     5941 gattatacga taccaaccgt tgtttttctt aaaaagtcaa ggttacccgg ttttgcaggc
     6001 tatgattaca agcaagatat tttatttgtg agcgatacac ttcattcaga aaaggaattt
     6061 gctaaagtct tatctgataa ttattttgcc gctcaaaaca ttaaagacac gatgattcat
     6121 gagttgacac acaaaaagca ttgggactct gctaaagcat tttacaaagc gcacaaaaag
     6181 cgttataata gtgtcgaaca agcaatgtct gaattaaatt cccctttggt gtcgtatgtc
     6241 aaagaacaat tgaagcatga ttttaattat ttggtaaata tcagcccaaa tgcggatgaa
     6301 gcttttattt taggggatgt aaatgaattg gtagctgaag tttcagtctt gggagaacgt
     6361 acttcagacc agatattgta ccaaaaagta caggaggtat tatcatggaa gtaatggcta
     6421 tgcctagtaa ggcgactgtg atgttttata atcaagtgcg tccttggatt gtttcagggg
     6481 aaatgaagaa tggtatcatg aattacaagt tcgcagatga tacccctaac aaagttttag
     6541 aattgttttc tgaactcaaa gacaaactta gttatccaag tgtcgctttg tgattggatt
     6601 caaatatttt aaataatcaa gcacctagag aaatctaagt
//
LOCUS       DATUCG010000120         6548 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.122, whole genome shotgun sequence.
ACCESSION   DATUCG010000120 DATUCG010000000
VERSION     DATUCG010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6548
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.122"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            <1..863
                     /locus_tag="VCA20_001977"
     CDS             <1..863
                     /locus_tag="VCA20_001977"
                     /inference="COORDINATES: protein motif:HMM:NF027528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SWF/SNF helicase family protein"
                     /protein_id="HEP1835733.1"
                     /translation="INMLNPALANKLESRYHSSKDLKIKLSTIYLRRKRKEVLDELPE
                     LETIELWSQFTPIQQQFYDESLLDGPGGIMRMRRAAFFGNDSKKILQILEICKEAKET
                     GQKVIIFSFFKEGVLYRLQDELKHLAHFIISGDISPKQRQETIDEFSKDPNQTVLLSQ
                     IDAGGVGLNIQAANIVILCEPQWKPSTEQQAISRVYRMGQSRKVIVYRLLTEESIDES
                     IVKLLSEKIELFNKYANDSSIAEAFEKSEVFEISNTEEKKLKQEVFELEKKRLALRKS
                     NTINSSSPNN"
     gene            948..1904
                     /gene="birA"
                     /locus_tag="VCA20_001978"
     CDS             948..1904
                     /gene="birA"
                     /locus_tag="VCA20_001978"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HEP1835734.1"
                     /translation="MKTYQKIYLLLKEREDYVSGEDLAQELGISRTSIWKAIRQLETH
                     GLTIEAARNRGYRLAEGDLLLPELISQELQLPVHLNMDSDSTQLDAKQGIESGHTSPA
                     LYLAPYQNKAKGRFGRPFYASKSGGIYMSLRLSPNVPFLEFKPYTILAAAAVVKAIQS
                     LCDLDVQIKWVNDIYLGHKKVAGILTEAISSMENQRVTDVIIGVGINVRIDDFPKGLQ
                     QSAGNLFEEQPPFTRNQLITAIWKAFLETDEKELIALYKEKSLVVGQQVSFVENQIEF
                     KGTAIAVTDTGNLVIQLDNGKAKIISSGEISLTSWSASPPNE"
     gene            complement(1870..2058)
                     /locus_tag="VCA20_001979"
     CDS             complement(1870..2058)
                     /locus_tag="VCA20_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HEP1835735.1"
                     /translation="MKLPQFLFLAITTILAVYFMNASILTGDFLIAGIYAFIAYRNLH
                     FAYKVTKFIRLVEKQTKK"
     gene            complement(2253..3920)
                     /gene="dnaX"
                     /locus_tag="VCA20_001980"
     CDS             complement(2253..3920)
                     /gene="dnaX"
                     /locus_tag="VCA20_001980"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEP1835736.1"
                     /translation="MYQALYRKYRSQTFGEMVGQEVVATTLKQAIEQGKISHAYLFSG
                     PRGTGKTSAAKIFAKAMNCPNQVGGEPCNDCYICQAITEGSLEDVIEIDAASNNGVDE
                     IRDIRDKSTYAPSLATYKVYIIDEVHMLSTGAFNALLKTLEEPTENVVFILATTELHK
                     IPATILSRVQRFEFKSIKVTDIQEHLAAILTKEGLIFDEQALTIIARRAEGGMRDALS
                     ILDQALSLSQDQQVTLEIAEEITGSISLCALDDYVASLRQGDSVTALQHLQTLFDQGK
                     SMSRFATDLLHYLRDVLIVQTGGEDTHLTTGFSENLALEQARIFTMIEMVTRGLADIK
                     SSPQPKIYAEMMTIRLAEDSSPSGAMAELPADLLGQLASLQQQVADLQKQLGQLSSQP
                     SAVKPVSRKPQAPKKYRLDTSKVHAILQEAMENPTLARENLTRLQNAWGEIIESLSGA
                     DRALLVGSQPVAANENHAILAFDSPLNAEQTMKRDNLNTMFGNILSKAAGFSPQILAI
                     AQEDWVSIRAEFSAKAKGQKTQEIEKEASPVPEEFGFLADTVEIRND"
     gene            complement(3920..4417)
                     /locus_tag="VCA20_001981"
     CDS             complement(3920..4417)
                     /locus_tag="VCA20_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEP1835737.1"
                     /translation="MTKEEKISNYQLLLAQLEALLDGETNALANLSNASALLNQALPN
                     SVFTGFYLYDGSELILGPFQGGVSCVHIALGKGVCGEAAAKRQTILVDDVRLHDNYIS
                     CDATALSEIVVPMVKNDQLLGVLDLDSRLVADYDTIDQDYLEKFVAFLVEKTDWNFAM
                     FGEKR"
     gene            complement(4515..6020)
                     /locus_tag="VCA20_001982"
     CDS             complement(4515..6020)
                     /locus_tag="VCA20_001982"
                     /inference="COORDINATES: protein motif:HMM:NF012948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEP1835738.1"
                     /translation="MKIKRSSSKNQLHKWTKLGVGLAAVTVLGGLVEPVGLADFGSTK
                     VYAEESKSITYRVHYFLNDTKENGGLPSYTDIAPYKEGTIIQGETITEIAPQIDGYVL
                     TKETWNSDKDVVTYDKMTAIEAWSDAEYVKSIMFFYDKVESSVVEKPAETPTEQPPQP
                     ITFPVRYVSVDYAADKGVVITGVLYETTATVEPGGTVIIENKTFDGYSLDTMEGPWKT
                     EHSYEDVVAAGWSDGIDLRYSANTPTIDNTTDTANSSVDVAFQEASIRLYNLLMTASR
                     LNPEDYTAESYNKYYDSHDEFGLYGTIISVLDEYKEVVNGNIKLEDRWDNGASPKAWL
                     AVVYNANSTVLEQALAGLVAVQQETPSDSSSAGTDEGTTGKTSQTPTDKPVTPPAETP
                     SVEPPATPPADSKPTETSPVTNQSTQNQPAQTGDLGSQTDKKPVSQPAQESQLAQLQV
                     KQATVKPASTSQSQAKSLPATGEASSVLHLTGLGLLGLAGLVVKRRPRKSS"
     gene            complement(6192..6461)
                     /locus_tag="VCA20_001983"
     CDS             complement(6192..6461)
                     /locus_tag="VCA20_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEP1835739.1"
                     /translation="MSIKQMVSMALVVWNLLVFITYGMDKGKARKNAYRISEKTLLLM
                     SYFGGGLGAWAGGTHFRHKTQKKYFQLAWAIGVLIDAVIMYWMWK"
BASE COUNT         1963 a         1447 c         1258 g         1880 t
ORIGIN      
        1 taattaatat gttgaatcca gctctagcaa acaaattaga atcaagatat cattcttcta
       61 aggacttaaa aataaagctt tctactatct acttaagaag aaaacgaaaa gaagttcttg
      121 atgaattacc tgaactagaa acgatagaac tgtggtctca atttacacca atccagcaac
      181 aattctatga tgaatcatta ctagatggac caggaggtat catgagaatg agaagggctg
      241 catttttcgg taatgattca aagaaaatac ttcaaatact tgaaatctgc aaggaggcta
      301 aagaaactgg tcaaaaggta attatctttt cattttttaa agaaggtgtc ctttatagat
      361 tacaagatga attaaaacac ttagctcatt tcataatttc aggtgatatt tcaccaaaac
      421 aaagacagga aaccatcgat gaatttagca aagatccaaa tcagacggtt ttactcagcc
      481 aaattgatgc tggaggagtc ggtttaaata ttcaagcggc caacattgtt atcctctgcg
      541 aacctcaatg gaaaccctcg accgaacaac aagcaataag ccgtgtctat agaatgggac
      601 aatccagaaa agttatcgta tatcggttgt taactgagga aagcatcgat gaatccatag
      661 taaaactact aagtgaaaaa attgaattat tcaataaata cgctaatgat tctagtattg
      721 ctgaagcatt tgaaaaaagt gaagtttttg aaatatcaaa taccgaggag aaaaaactaa
      781 aacaagaagt atttgaatta gagaaaaaaa gattggcatt acgaaaaagc aacacaataa
      841 atagtagttc gcctaataat tagttctaag tgtcaacaaa tatgattatc tctaatttct
      901 aattatatca tgtgatagag ccacaaatac tgtgctataa tggaggtatg aaaacctatc
      961 agaaaatata tctcctttta aaagagagag aggattatgt cagcggagaa gatttggcac
     1021 aggaattggg catttcacgg acatccattt ggaaggccat tcgccagtta gaaacgcatg
     1081 gtttaactat tgaggctgcc cgtaatcgtg gctacagatt agccgaaggc gatttactcc
     1141 tacccgaatt aatctctcag gaacttcaac tacctgtcca tctgaatatg gatagcgact
     1201 ccacccagct agatgccaaa caaggtattg aatccggtca tactagtcct gccctatatc
     1261 tagctcccta tcagaataaa gccaaggggc gatttgggag acctttttat gcatccaagt
     1321 caggtggcat ttatatgtcg cttcgtctct ctcccaatgt tccctttcta gaatttaagc
     1381 cctacaccat tttagctgca gctgctgttg tgaaggccat tcaatctctt tgcgacttgg
     1441 acgtccaaat caagtgggtc aatgacatct atctcggaca caaaaaggtc gctggcatcc
     1501 tcactgaggc tatttcttct atggaaaacc aacgggtaac cgatgtcatt atcggtgtcg
     1561 gaatcaatgt ccgcatagac gatttcccta agggattaca acaatctgca ggaaatcttt
     1621 tcgaggaaca accaccattt acccgcaatc aactcattac tgccatctgg aaagccttct
     1681 tagaaaccga tgagaaggaa ctaattgccc tttacaaaga aaaatcactt gtcgttggcc
     1741 aacaagtgag ctttgtagaa aatcaaattg aatttaaggg tactgccatc gctgttactg
     1801 atacaggaaa cctggttatc cagttagata acggcaaagc aaaaattatc tccagcggag
     1861 aaatcagcct tacttcttgg tctgcttctc caccaaacga ataaatttag ttaccttgta
     1921 agcaaaatga agattccgat aggcgataaa ggcgtagatc cctgcaatca agaaatctcc
     1981 tgtcaaaatc gatgcattca taaaataaac agctaaaata gttgtaatgg ctaaaaaaag
     2041 aaattgtgga agtttcataa aatagattat accaaaaatt cagtcaactg gctgaatttt
     2101 tatatgcttt cttgataggt ttcgattctt ttctaaacaa gaaaacacca atctaattgg
     2161 tgctttctta aggagatatg aaaaatattt aggattgaat atagtataaa atatctatca
     2221 aaactattca tcagtcttag gactgatatt ttctaatcat ttctaatctc aactgtatct
     2281 gcgagaaagc caaattcttc tgggactgga ctggcttctt tttcgatttc ctgtgtcttt
     2341 tgcccttttg ctttcgccga aaattcagca cgaatactta cccaatcttc ctgagcaata
     2401 gctaaaatct gtggagaaaa acctgcggcc ttgctgagaa tatttccaaa catggtattg
     2461 agattgtcac gcttcatcgt ctgttcagca ttaagcggag aatcaaaggc tagaatcgca
     2521 tgattttcat tggcagcaac tggttgagaa ccaacaagca aggctcgatc tgctcctgac
     2581 agactctcga taatttctcc ccaagcattc tgcaagcgag tcaaattttc acgcgccaag
     2641 gttggatttt ccatagcttc ctgcaaaata gcatgaacct tgctggtatc caatcggtac
     2701 ttcttcggag cctgtggctt gcgtgaaact ggcttaacag cacttggttg actagataat
     2761 tgacctagtt gtttctgtaa atccgcaact tgttgttgca gactggctag ttgacccagt
     2821 aaatcagctg gcagctctgc catggccccc gaaggagaac tatcttcagc caaacgaatg
     2881 gtcatcattt cagcgtaaat cttcggttga ggactggact tgatgtccgc caaacccctg
     2941 gtgaccatct ctatcatggt aaaaatccgt gcctgctcaa gagctagatt ttctgaaaaa
     3001 cctgttgtta aatgcgtatc ctcaccaccc gtctgcacga taaggacatc tctcaaatag
     3061 tgcaaaagat ctgtcgcaaa acggctcata ctctttcctt ggtcaaacaa ggtttgcaga
     3121 tgctggagag ctgtgacact atccccttga cgcagacttg ccacatagtc atccaaggca
     3181 cacaagctga tagaccccgt aatttcctca gcaatttcca aggtcacttg ctggtcctga
     3241 ctaaggctga gagcctgatc caaaatggat aaggcatccc gcatccctcc ttctgcccga
     3301 cgcgcaatga tcgtcagagc ctgctcgtca aagattagcc cttccttggt caaaattgca
     3361 gccaagtgct cctgaatgtc cgtcaccttg atggacttaa attcaaatcg ttgcaccctt
     3421 gacagaatgg tagcaggaat cttgtgcaat tccgtcgttg ctagaataaa gaccacattt
     3481 tcagtcggtt cttccaaggt cttcaagagg gcgttgaagg ctccagtaga cagcatgtgg
     3541 acctcgtcga tgatatagac cttataggtc gcaagactag gtgcataggt cgatttatca
     3601 cgaatatcac gaatctcatc cacaccattg ttggaagccg catcaatctc aatcacatct
     3661 tccaggctac cttctgtaat agcctgacag atataacaat cgttacaagg ctcgcctcca
     3721 acctgattag gacagttcat ggccttggca aaaatcttag ctgccgatgt cttacccgta
     3781 ccacgaggac ctgaaaagag ataggcatga ctgattttcc cttgttcaat ggcctgtttg
     3841 agcgtggtcg caaccacctc ctgccccacc atctccccaa aggtctggct tcggtacttc
     3901 ctatataaag cttggtacat tagcgttttt ctccaaacat agcaaaattc cagtccgtct
     3961 tctcaaccaa gaaagcaacg aacttttcta gatagtcttg gtcaatcgta tcataatcag
     4021 ctaccaatct cgaatccaag tccaaaacgc ccagcaactg gtcgtttttc accattggca
     4081 caacgatttc tgatagggct gtcgcatcgc aggaaatata gttatcatgt agacgaacat
     4141 cgtctacgag aatagtctgg cgcttagctg cagcctcacc gcagacaccc tttccaagtg
     4201 caatatgaac acaggaaacg ccaccctgaa aaggccccaa aatcaattca ctaccatcat
     4261 acaagtaaaa tcctgtaaag acagagtttg gtagagcttg attgagcaaa gcagaagcat
     4321 ttgatagatt ggctaaagca ttggtttcac catcaagaag agcttccaat tgagccaata
     4381 atagttgata atttgaaatt ttttcttctt tagtcataaa aacattatag catagaaaga
     4441 cggagcttga cagagccgac tgttagaaat cagcaaaaaa tgagacctgt cgatctcgtt
     4501 tttaggatag ctaattaaga cgacttgcgt gggcgtcttt tcacgactag acctgctagt
     4561 cccagtaagc ccagacctgt caggtgtagg acggagctgg cttcaccagt agctggtaaa
     4621 ctttttgcct gtgactgact agttgatgca ggtttgacag ttgcctgttt gacttgaagc
     4681 tgtgccaact gactttcttg agcaggttga ctgactggtt tcttatcagt ttgggaacca
     4741 agatcacctg tttgggctgg ttgattttgt gttgactgat tggtaactgg acttgtctct
     4801 gtcggcttgc tgtcagctgg aggggttgct ggtggctcga ctgaaggagt ttcagctgga
     4861 ggggtgactg gtttgtctgt cggagtttgc gaagtttttc cagtcgtccc ctcgtctgta
     4921 cctgcagaac tgctgtcact tggtgtctct tgctgaacag ctactaatcc tgccaaagcc
     4981 tgctctaaaa cggtactatt agcattgtat actaccgcca accatgcttt tggtgaagct
     5041 ccattatccc atctatcctc tagtttaata ttcccattga ctacttcttt atattcgtcc
     5101 agaacagata taattgttcc atataatcca aactcatcgt gtgaatcata gtatttatta
     5161 taggattctg ctgtatagtc ttctggattt aagcgactag ctgtcatcag caagttgtag
     5221 agtcggatac tagcttcttg gaaagcgaca tccactgaac tattagccgt gtcggtggta
     5281 ttatcaatag ttggtgtatt agcagaatac cgaaggtcaa ttccatctga ccagcctgca
     5341 gctacaacat cttcatagct atgctcggtt ttccaaggtc cttccattgt atctagacta
     5401 taaccatcga atgttttatt ctcaataatg acagttccgc caggctcaac agtagccgtt
     5461 gtttcataga gaacacctgt aatgaccaca cctttatcag cagcatagtc aacggaaaca
     5521 tagcgaactg ggaaagtgat tggctgaggc ggttgctccg ttggtgtttc agctggcttt
     5581 tcaacaacgg aagactcaac cttatcgtag aagaacataa tggacttaac atattctgca
     5641 tcactccatg cctcaatagc cgtcatttta tcgtaagtaa caacgtcttt atcactattc
     5701 caagtttcct ttgttagaac atatccatct atctgtggtg caatttcagt tattgtttct
     5761 ccttgtatga tagttccttc tttatatgga gcgatatctg tataggatgg taagcctcca
     5821 ttttctttag tgtcatttag gaaatagtga acacggtaag ttattgactt actttcctca
     5881 gcataaacct tggtcgaacc aaaatctgcc aaccccaccg gctcaaccaa gcctcccaaa
     5941 acagtgaccg ctgctaatcc aactcctagc ttggtccact tatgaagttg gttcttgcta
     6001 gaacttcttt tgattttcat actaaaaacc tccatttata taaaactttt atgccaaaag
     6061 gcacgcttac cagtatactt aaaaaaaaaa gtaatctgtc aagaaattat gcgcttaata
     6121 ttcaaatatt cggttctttc atggttttca gtcgattttt catattttcg gaaaaagaaa
     6181 aaggcttgcg cctatttcca catccagtac ataatcactg catcaatcaa tacccctata
     6241 gcccaggcta gttgaaaata ctttttctgt gtcttatgcc gaaaatgggt tccacctgcc
     6301 caggcaccca gcccgccacc aaaataggac atgagtaata aggtcttttc tgaaatccga
     6361 taggcgttct ttcttgcctt acctttatcc ataccatagg tgatgaagac aagtaaattc
     6421 cacactacta gcgccatact aaccatctgt ttgattgaca tacattcctc caattaagtc
     6481 acatttcttc ttactctaac ataatccaaa acaggacaca agaaaaaacg attcggaaat
     6541 ccgaatcg
//
LOCUS       DATUCG010000121         6453 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.123, whole genome shotgun sequence.
ACCESSION   DATUCG010000121 DATUCG010000000
VERSION     DATUCG010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6453
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.123"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            <1..485
                     /locus_tag="VCA20_001984"
     CDS             <1..485
                     /locus_tag="VCA20_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HEP1835740.1"
                     /translation="VVEAPVEEVVEQPAVEAPVEEVVEQPVVETPQVTALSTTTTSTS
                     AYDVGLQPQVAAFRAEVANAFGITSFSGYRPGDSGDHGKGLAIDFMVPQSSALGDQVA
                     EYAIANMGAKNISYIIWKQRFYAPYASIYGPAYTWNLMPDRGSITENHYDHVHVSFNP
                     "
     gene            complement(724..1056)
                     /locus_tag="VCA20_001985"
     CDS             complement(724..1056)
                     /locus_tag="VCA20_001985"
                     /inference="COORDINATES: protein motif:HMM:NF020397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEP1835741.1"
                     /translation="MRQEFLNKVKNPVLKDRLEAIFAQIQQEFPSLTTEIKWNQPMFI
                     MDGTFIIGFSSAKAHISIAPETVTMETFAKDIKEAGSEATSNLFKIKEDQEVNWDLLR
                     NIIAFIVD"
     gene            complement(1114..1866)
                     /locus_tag="VCA20_001986"
     CDS             complement(1114..1866)
                     /locus_tag="VCA20_001986"
                     /inference="COORDINATES: protein motif:HMM:NF014566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEP1835742.1"
                     /translation="MKPLTKLTCIGFCTLAFWAMMTSGLIAFLPQLALGDNIVTTVLR
                     AGLYIFLYYLFAKWLSRRLGMSLIQFRITLVKPSQFWLVVAIFLPLTVLLVLYLLGGK
                     VTLTDDLVNSIFMGAIAAPIVEETCFRGIMMHLLEQSFSRKVAILGPSVFFGLVHLLN
                     GSVSSLTALQLLIAGTLVGSLFSLATLQTGSILSSILVHSLWNLSTSILLFDNQTSLF
                     GGEYGVDVSPIASLAYLIVILLSMIAHRKKAS"
     gene            complement(1850..2500)
                     /locus_tag="VCA20_001987"
     CDS             complement(1850..2500)
                     /locus_tag="VCA20_001987"
                     /inference="COORDINATES: protein motif:HMM:NF012655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEP1835743.1"
                     /translation="MAKSKSPDENRQLILDVARTLFLEKGYDQTSLTDIISQLGGLTK
                     GVVYHHFPSKGAILDALIGDADENLLSKEWPGNCAIEKIQYALITSLSDVEKLSFLQA
                     SQITLKSPNILAQQYELSYSLLIPKFQEVVRQGVTDGSISTDFPEEIAELLVVYFNLI
                     IGLRITELSPDDFLRKILFLKKLFESMKTPIISDKILALASDLSTKIGGQINETTH"
     gene            complement(2582..4207)
                     /gene="treC"
                     /locus_tag="VCA20_001988"
     CDS             complement(2582..4207)
                     /gene="treC"
                     /locus_tag="VCA20_001988"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEP1835744.1"
                     /translation="MTIDKRKVVYQIYPKSYKDTTGNGVGDLRGIIEKLPYLKELGID
                     MIWLNPFYPSPQRDNGYDISDYTAVNPDFGTMADFEEMVAVGKELGIEFMLDMVLNHC
                     STNHEWFQKALAGDKYYQDFFILRDQPTDWVSKFGGNAWAPFGDTGKYYLHLFDVTQA
                     DLNWRNPHVREELFKVVNFWKDKGVKGFRFDVINLIGKDEVLEDCPINDGKPAYTDRP
                     ITHDYLKMMNNATFGAEKSFMTVGEMSATTIENCILYTAPEREELSMAFNFHHLKVDY
                     KDGQKWTIMDFDFEELKRLFHTWGEEMSVGNGWNALFYNNHDQPRSLNRFVDVENFRK
                     EGATMLAASIHLSRGTPYIYMGEEIGMIDPDYDSMDDYVDVESINAYQMLLDQGHAPE
                     QAFKIIQAKSRDNSRTPMQWDASDNAGFSTGTPWLKAGKSYQTINVKQEKTGPIFTFY
                     QELIRLRKELPLISEGDYKAAYKDSQKVYAFERLLNGEKLLVLNNFFAEEVELDLVDD
                     YAHGQVLISNYPDNKLGKKITLKPYQALAILVK"
     gene            complement(4288..5388)
                     /locus_tag="VCA20_001989"
                     /pseudo
     CDS             complement(4288..5388)
                     /locus_tag="VCA20_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636547.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
     gene            5548..5919
                     /locus_tag="VCA20_001990"
     CDS             5548..5919
                     /locus_tag="VCA20_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000591175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 family protein"
                     /protein_id="HEP1835745.1"
                     /translation="MKYIIDAIIRGIIPFVIMSGISLIMVYQKLNSFQIKSTFLVGII
                     ITLVSAASVIYEIDNLTLTQQSIIHFFVMLITVFPCLIISGWFPLSNIFDFLKLLAIF
                     LVVGVVLWIISFFIFGKLLNK"
     gene            5983..>6453
                     /locus_tag="VCA20_001991"
     CDS             5983..>6453
                     /locus_tag="VCA20_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEP1835746.1"
                     /translation="MTKIGYARVSSKEQNLDRQLQALQGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVVVTELDRLGRNNKELTELMNAIQQKGATLEVLNLPSMNGIEDENL
                     RRLINNLVIELYKYQAESERKRIKERQAQGIEIAKKKGKFKGRQLKFKKNDPRLK"
BASE COUNT         1859 a         1221 c         1457 g         1916 t
ORIGIN      
        1 ctgtggttga ggcacctgta gaggaagttg tagaacagcc tgcagtagaa gcgcctgtag
       61 aggaagttgt agaacagcct gtagttgaaa ctccacaagt gacagcccta tcaaccacta
      121 caacaagtac aagtgcttat gatgtcggtt tgcaacctca ggtagcagcc ttccgcgcag
      181 aagtagctaa tgccttcggt attacttctt tctcaggtta ccgtcctggt gattctggcg
      241 accatggtaa gggattggca attgacttca tggtaccaca aagttcagca cttggggatc
      301 aagtggcaga atatgctatt gccaatatgg gtgcgaaaaa tatttcctat attatctgga
      361 aacaacgttt ttatgctcca tatgcaagta tttatggacc tgcatatact tggaacttga
      421 tgccagaccg tggaagtatt actgaaaacc attatgacca cgtacacgtg tcatttaatc
      481 cataaaataa ttatagccaa actcacggtg agcttggcta tttttgcgtg cttattaatt
      541 ggaaggtgtg gcatacaagt ttcttatttt atctccagaa cttttcgtaa cctttctttt
      601 cctccatatt atagtcaagt gaattagaaa caggacatat ctaagtttgt caatttgaga
      661 actctaaagt tattttagga gtgggttatt aacactataa cttaaggctg tccttttcct
      721 aattcaatcc actataaagg cgataatatt gcggagcagg tcccagttga cctcttgatc
      781 ttccttgatt ttgaagaggt tggaagttgc ttcggagccc gcctccttga tgtccttggc
      841 aaaggtttcc atagtaaccg tttcaggtgc aatggaaata tgggccttgg cagaagagaa
      901 tccaatgata aaagttccgt ccatgataaa catgggctgg ttccacttga tttctgtggt
      961 tagacttggg aattcctgct ggatttgggc gaagattgct tcgagcctgt ccttcaagac
     1021 aggattcttg accttgttca aaaattcttg gcgcatgatg tttcctttct tttttacagg
     1081 ataacataga agagtaaaat ggtgtagaaa caatcagctt gcttttttcc gatgagcaat
     1141 catactaaga aggatgacga tgagataggc tagacttgca attggagaaa cgtcaactcc
     1201 gtattcacct ccgaacaagc tggtttgatt gtcgaaaaga aggatggaag tggagagatt
     1261 ccaaagggag tggactaaaa tactggatag aatacttcct gtttgcaaag tagcaagcga
     1321 aaataaactt ccaactaagg tgccagcaat gagtaattgt agggcggtga gtgagctaac
     1381 ggatccgttt aataggtgaa caagtccgaa aaatacggac ggtcccagaa tagcgacttt
     1441 tcttgaaaaa gattgttcta gtagatgcat catgatacca cggaagcacg tctcttctac
     1501 aatcggagca gcgatagcgc ccataaagat agagtttact aaatcgtccg tcaaagtcac
     1561 ttttccacct agtaaataca aaacgagcaa gacagttagt gggagaaaga ttgcaacgac
     1621 taaccaaaat tgacttggtt taacgagtgt tatccgaaat tgtattaggc tcattcctaa
     1681 tcgacgagat agccatttgg caaataaata atacaagaag atgtagagcc ctgccctgag
     1741 tacagttgta acaatattgt caccaagtgc cagttgcgga agaaaggcaa tcaggccact
     1801 tgtcatcata gcccaaaacg caagtgtaca aaatccgata catgttagtt tagtgagtgg
     1861 tttcatttat ttgtcctcca atttttgtag aaaggtcaga tgcgagagca aggattttat
     1921 cgctgataat aggggtcttc atgctttcaa aaagtttttt taagaagaga atttttcgta
     1981 gaaagtcatc gggagagagt tccgtaattc gtaggccaat aatgaggttg aaatagacaa
     2041 ccagaagttc ggcaatttct tcaggaaaat cagttgaaat acttccgtct gttacacctt
     2101 gtctcacaac ttcttgaaat ttcggaataa gcagtgaata ggatagttcg tactgttgtg
     2161 ctaaaatgtt aggacttttc agggtaatct gcgatgcctg caagaaggat agtttttcaa
     2221 catcagaaag cgaggtgatg agagcgtatt ggattttctc aatcgcacaa tttcctggcc
     2281 actcttttga taataaattc tcatcagcat ctccaatcag agcatccaga atggctcctt
     2341 tggaggggaa gtgatgatac acaactccct tggtcaggcc acctagttga gaaataatat
     2401 cagtaagact ggtttggtca tagccttttt ctaaaaataa tgttcttgca acatcgagga
     2461 tgagctgtct gttttcgtca ggggatttac tttttgccat tccgttctcc atttacatac
     2521 cgtcggtatg taaagagtat atcaaaaatt tccctacttt acaataggga atatacagtg
     2581 attatttaac caaaatcgcc agtgcttgat aaggcttgag ggtgattttc ttacctagtt
     2641 tgttgtcagg atagttgctg ataaggactt ggccgtgggc gtaatcgtct accaggtcta
     2701 gttcgacctc ttctgcgaag aaattgttga ggactagcag tttttcaccg tttagcaagc
     2761 gttcaaaggc gtagactttc tggctatctt tgtaggctgc cttgtaatct ccttctgaga
     2821 tgagggggag ttcttttcgg agtcgaatca actcttgata gaaggtaaag attgggccgg
     2881 ttttttcctg tttaacgtta attgtttggt aggatttgcc agctttcagc caaggagttc
     2941 ctgttgagaa gcctgcattg tcagaagcgt cccattgcat cggggtgcgg gaattgtcac
     3001 gggacttggc ctggataatc ttgaaggcct gctcaggtgc gtgaccttgg tccaagagca
     3061 tctgataggc attgatagat tccacgtcca cgtagtcgtc catactgtcg taatcggggt
     3121 caatcatacc gatttcctcg cccatgtaaa tgtaaggcgt gccacgtgac aggtggatag
     3181 aggcagccag catggttgct ccttccttgc ggaaattctc cacatcaaca aagcggttga
     3241 gggaacgagg ttggtcgtgg ttgttgtaga aaagggcatt ccagccattt ccaacggaca
     3301 tctcttcgcc ccaagtgtga aagaggcgtt tgagttcttc aaagtcaaag tccatgatgg
     3361 tccatttttg cccgtccttg tagtccactt tcaagtgatg gaagttgaag gccatggaca
     3421 attcttcccg ctcaggagct gtgtagagaa tgcagttttc aatggtggtt gcagacattt
     3481 ccccgacggt catgaagctt ttttctgccc caaaagtggc attgttcatc atcttgaggt
     3541 agtcgtgggt aattgggcgg tcggtataag ctggcttgcc atcattgatt gggcaatctt
     3601 ctaggacttc gtccttacca atcaggttaa tgacatcgaa gcggaagcct ttgacaccct
     3661 tgtctttcca gaagttgacc accttgaaca gttcttctcg gacatgaggg tttcgccagt
     3721 tgaggtcagc ttgggtcaca tcaaataggt ggaggtagta tttcccagta tctccaaaag
     3781 gagcccaggc attgccgcca aatttagaca cccagtcagt tggctggtca cgcaggataa
     3841 agaaatcctg gtagtatttg tcgccagcta gggctttttg gaaccattcg tggttggttg
     3901 agcagtgatt gaggaccatg tccagcatga attcaatacc cagctctttg ccgacagcca
     3961 ccatttcttc aaagtcagcc atggtgccaa aatcaggatt gacggctgta tagtctgaaa
     4021 tgtcatagcc gttgtcccgt tgaggacttg ggtagaaagg gttgagccaa atcatatcaa
     4081 tacctagctc ttttaaatat gggagttttt caataattcc tcgtaaatcc cctacaccat
     4141 ttccagttgt atccttgtaa gattttgggt agatttgata aacaaccttt cgtttgtcta
     4201 ttgtcatttt gctctctctt tcttgatttt tccttcttgc ataagcaatg ataggaggtg
     4261 caagttacct tgcgccctcc tagaacatta attctttgta actgtcagaa tagcttcgcc
     4321 ttgcgagatg gcacgaggaa gatttccgat aacttgagtg tcaaagtcag tttggttggt
     4381 cacgataact ggtgtttcag cgatgagacc agcagcctta atgacatcga tgtcaaagct
     4441 aatcaattta tcaccagctt ttaccttgtc gccttgtttg acgtgagctg tgaagccttt
     4501 gccttccaat ccaacagtat ccataccaat gtgcataagg agctcaactc cgtttgtagc
     4561 agtgatacca acggcatggt tagttgggaa gaggacagat acttcaccat cgactggagc
     4621 aaccaagata ccttcgctcg ggtcaataag gacaccttgg cccatgacac ctgatgcaaa
     4681 tacagggtca gtagcttgtg acaattcttt ggtttgacca gtaagtgggc taatcaattc
     4741 gatagcagcg ccagtttcaa caactgcttc agttggagct acaacttctt caacagtttc
     4801 ttcttttttt gaaaatgcac ctgtacgttt gaaaacagct gtcaaaatca ttgggacaac
     4861 aatcgcaaca atcattgtca taaagaatgc acaccagtat tcagccttga tagacaagat
     4921 acctggaaga ccaccgatac cgatagaagc tgcttggatg ttgaaggtta cagagagtaa
     4981 gcctgcgata cttgaaccaa tcatagcagc tacgaatggg taaatgtatt ttacgttgac
     5041 accaaagagg gctggttcag taacgcctag gtaggcagaa atagttgcag gaagtgaaac
     5101 ttgcgcttct ttttcattgt gacggttcat gaggaagtag gcaaatactg ctgaaccttg
     5161 agcaatgtta gaaagagcaa tcattggcca gagaccagtt ccaccagcat ccgcaatcaa
     5221 ttgtgtatct atggcgtttg tcatgtggtg aagaccagtg ataacgaatg gcgcatagag
     5281 ggcaccgaat accgcaccga agagccattt caatgggcct gtcaaacctg ccaatacaac
     5341 tgttgacaac cattgcccga ttgtccaacc gataggacca aggacagtga gagcgaagcg
     5401 aacacttgat tttttaagtt gctatcattt ataggtcaat agagtatact tgattgtcct
     5461 ttttagtaag tattgatttt ttgtggtata ctgggattag caaataggac atttaagttg
     5521 ggcaaaatta gagtggaggt gtcaaaaatg aaatatatta tagatgcaat cataagggga
     5581 attattccct ttgtaattat gagcggaata tctttaataa tggtttatca gaagttgaac
     5641 tcatttcaaa taaaaagtac ttttctagta ggaataatta ttacattagt ttcagctgct
     5701 agcgtgattt atgagattga taatttaact ttaacacagc aatctattat tcactttttt
     5761 gtaatgctga taacagtttt tccttgtttg ataattagtg ggtggtttcc attgtcaaat
     5821 atctttgatt tcttaaagtt attggcgatc ttcttggtag ttggcgttgt tttatggata
     5881 atttcatttt ttatttttgg aaagctacta aataaataag tttagcttat atatttgctt
     5941 gtggaattta aaaaaacttt ttttaaaaaa ggaaagatgt taatgactaa aatagggtat
     6001 gcacgagtaa gtagcaaaga acagaactta gatcggcaat tacaagcgtt acagggcgtt
     6061 tctaaggtct tttcagacaa attaagcggt caatcggtcg aacgtccaca attacaagct
     6121 atgcttaact atattcgtga aggggatatt gttgtagtta ctgaattaga tcgattagga
     6181 cggaataata aagaattaac agaattgatg aatgccattc aacaaaaagg agcaacgctt
     6241 gaagttttga atttgccctc tatgaatggg attgaagatg aaaatttaag gcgtttgatt
     6301 aataatttag tgattgaatt atataagtat caagcggaat cagaacgcaa acgtattaag
     6361 gaacgtcagg cacaaggaat cgaaattgct aagaaaaaag gcaaattcaa aggtcggcaa
     6421 ctgaaattta aaaaaaatga tccacgttta aag
//
LOCUS       DATUCG010000122         6444 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.124, whole genome shotgun sequence.
ACCESSION   DATUCG010000122 DATUCG010000000
VERSION     DATUCG010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6444
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.124"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            64..1854
                     /locus_tag="VCA20_001992"
     CDS             64..1854
                     /locus_tag="VCA20_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEP1835747.1"
                     /translation="MKKTTKLFALAGVTLLSASVLAACGSKQSGAAKQELSFPAEVKQ
                     DGTAVSDAQLKYAWVSATTSSGLLIDELTENTTDSTFGGFVDISLFGYDGDRKLDDSG
                     LAKAEFDVEGKKITVSLTGKDYKWSDGQPFTINDYIFTIKSMASKDYTGVRFDDKFLN
                     IVGMDEFVAGTASDISGLKKIDDYTVELTVKEMSPSMMYAGGDVPAYIQPEHIYKDIP
                     VADWEKSEYSRTAKLVGMGPWKIKEIVNGESITYVPNEYFFKGTTPKTSSLKIDIVSP
                     DTIVSEMKAGNYDIAEMPVDQLDSYKDVSNLNIVGQLESSYEYISFNLGKYDDSAKKN
                     VMDENAKMNDVKLRQAIAYAIDTKTAGESLYNGLYHPAKSLIISFFGDIHDSELEGYS
                     YDPEKAKKLLDEAGYKDVDGDGIREGKDGKEFKITFAARKRTEANEALVQQYIAWWKE
                     VGLNVELYTGRTVEGNTFYEAVQANDPAIDMYAGGWSTGYDPNPTGLWGPIAAFNMSR
                     FVSDENTKLLNAISSKESFDDKKNVENYKAWQKYAYEQAFAIPTFESEKVTALNKRVK
                     NYDTNYGSASGNGIAYEGIELTADKGVVAE"
     gene            2230..3228
                     /locus_tag="VCA20_001993"
     CDS             2230..3228
                     /locus_tag="VCA20_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1835748.1"
                     /translation="MATEKPLLDIKDLHVGFRIGDDYFDAVDGVDISLQKNEILAIVG
                     ESGCGKSTLATTIMGLHNPLNTKITGSIQYNDMELIGMDEAKYNTVRGNDIGMIFQDP
                     LASLNPLMTIGAQIDEALFYHTDLDANARTERVLELLAQVGIPNPKRTFKQYPHELSG
                     GMRQRIIIAMALSCKPPIIIADEPTTALDVTIQAQILDLLNDIQAETGSGIILITHDL
                     GVVAETADRVAVMYGGQFVEVAPVEELFTNPKHPYTRSLLKSNPQSGSEGGDLHVIEG
                     IVPPITKMVRKGCRFAPRIPWIGAEAHEENPSMHEVGPNHFVRCTCHETFYFEGEA"
     gene            3231..4172
                     /locus_tag="VCA20_001994"
     CDS             3231..4172
                     /locus_tag="VCA20_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1835749.1"
                     /translation="MGFIEVKDLKVHYPIRSGFFNRVTDHVYAVDGVNIEFEEGKTYG
                     LVGESGSGKSTIGKAIIGLERATSGQIIYEGQNVTNKSRGKKGNFNRDVQMIFQDSLS
                     SFNPKKRILDIIAEPIRNFDRLSPDEEKKKVLHLLDTIGLNEEALIKYPHEFSGGQRQ
                     RIGVARALASNPRLIIADEPVSALDLSVQAQVLNYMKCIQDEFKLSYLFISHDLGVVQ
                     HMCDELFIMYRGRFVETGNKNDIYNNPQHIYTKRLLSAIPTIDPVNRLKNKEKRLAAE
                     KEYQEMQGQYYDENGRVYDLRSFSETHQVALPEGGQN"
     gene            4174..5130
                     /locus_tag="VCA20_001995"
     CDS             4174..5130
                     /locus_tag="VCA20_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP1835750.1"
                     /translation="MWKTVLRRLLMMIPQIIILSIIAFFVAKMMPGDPFTGLIDPNID
                     PAIIEQKRIAAGYYDPIPVQYFRWIGNLLQGDFGQSILFKQPVVDVIMQRLNNTIWLS
                     LLTMVLTYLIALPLGMIAGRYQNSLADKIIGVYNFLTFSTPTFVFAILMLWMFGFSLG
                     WFPTRGSIDGGVEGFAAILSRLHHMILPAITMAILSTTVTIQYLRTGIIDAKSQDYVR
                     TARAKGVPERVVYNRHIFRNSILPIASFLGYELTGLIGGSIFIENIFTYPGIGQLFFN
                     SISSRDYSVILALLLIFGMGTLLGTLISDIIMSIVDPRIRVK"
     gene            5144..6046
                     /locus_tag="VCA20_001996"
     CDS             5144..6046
                     /locus_tag="VCA20_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP1835751.1"
                     /translation="MSKENKKQVQSASTPPGGFSVIAREFMKDRLALTSLIILVTVLL
                     AVFIGALLLDQEQVMKVNLLGRYLEPGVNGYILGTDEGGKDIFGQLIIGARNSIIIGF
                     TITIITSIVGISVGLISGYYGGRLDGFLMRIVDFIMILPVTMLIIVFVTVIKNYTIYH
                     FILIMSAFYWTAKARLFRTRTLSEASLDYVNASKTLGTSDFMIMFREILPNLSSLIIV
                     NLTLNFAGNIGIETSLTYLGFGLPSTVPSLGTLIANARNADILENKTWIWLPAAIFIL
                     VMMLCINYVGQAFQRAADAKQRLG"
     gene            complement(6202..>6444)
                     /locus_tag="VCA20_001997"
     CDS             complement(6202..>6444)
                     /locus_tag="VCA20_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HEP1835752.1"
                     /translation="FNVCKWLTSHVTIKTSKLKQTYKICFSDAVYACRKFLREELTSF
                     QLETYIAKHLSIIRPNRTFQRKIKSQAPVSFTYRIS"
BASE COUNT         1891 a         1143 c         1422 g         1988 t
ORIGIN      
        1 gatataatta tttttataag acatagtaat aagtgtcaaa caaaaagaaa acagaggtgt
       61 tttatgaaaa agacaacgaa actttttgca cttgcaggtg tgactctcct ttcagcatct
      121 gttcttgcag catgtggttc aaaacaatca ggtgcagcaa aacaggaatt gtcattcccg
      181 gcagaagtaa aacaagacgg aacagcagtg tctgatgctc agttgaaata cgcatgggta
      241 tcagcaacta cttcatcagg tctcttgatt gacgagttga ctgaaaatac aactgactca
      301 acatttggtg gttttgttga tatctcactg ttcggttacg atggagatcg taagttggat
      361 gattctggtc ttgctaaagc tgaatttgat gtagaaggta agaaaatcac agttagcttg
      421 acaggtaagg actacaagtg gtcagatggt caaccgttca caatcaacga ctacatcttt
      481 actatcaagt caatggcaag caaagactat acaggtgttc gttttgatga taaattcttg
      541 aacattgtgg gtatggatga atttgttgct ggaactgcat cagacatctc tggtcttaag
      601 aaaattgatg attacacagt ggaattgact gtaaaagaaa tgtcaccttc aatgatgtat
      661 gccggtggtg atgtacctgc ctatatccaa ccagaacata tctataaaga catccctgtt
      721 gcagattggg aaaagagcga gtactcacgt acagctaaac ttgttggtat gggaccttgg
      781 aaaatcaagg aaatcgtaaa cggtgagtct atcacttacg taccaaacga atacttcttc
      841 aaaggtacaa cgccaaaaac aagttcattg aagattgaca tcgtatcacc agatacaatc
      901 gtttctgaaa tgaaagctgg taactatgac attgctgaaa tgccggttga tcagttagat
      961 tcatataagg atgtttctaa cttgaatatc gttggtcagt tagagtcgtc ttatgagtat
     1021 atctcgttta atcttggtaa atatgacgac tctgctaaga aaaacgtcat ggatgaaaat
     1081 gctaagatga atgatgtgaa acttcgtcaa gcgattgcct atgcgattga taccaagaca
     1141 gctggtgagt cattgtacaa tggtctttac caccctgcaa aatcattgat tatctcattc
     1201 ttcggcgata ttcatgattc agagttggaa ggttattcat acgatcctga aaaagcgaaa
     1261 aaactcttgg atgaagctgg ctacaaagac gtagatggcg atggtatccg tgaaggcaaa
     1321 gacggtaagg aattcaagat tacctttgct gctcgtaaac gtactgaagc caacgaagcg
     1381 cttgtacaac agtacattgc ttggtggaaa gaagttggct tgaacgttga actttacaca
     1441 ggccgtacag ttgaaggcaa tacattctac gaagctgttc aagcaaatga tcctgctatc
     1501 gatatgtacg ctggtggttg gtcaacaggt tatgatccaa acccaactgg tctttggggt
     1561 cctatcgctg ccttcaatat gtcacgtttc gtgtctgatg aaaacactaa attgttgaat
     1621 gcaatcagct caaaagaatc atttgatgat aagaaaaatg ttgagaacta taaggcttgg
     1681 caaaaatatg cttacgaaca agcatttgct attccaacat ttgaatctga aaaggttaca
     1741 gcacttaaca aacgtgtgaa aaactatgac actaactatg gttcagcttc aggaaacggt
     1801 attgcttatg agggtatcga acttacagct gataaaggtg tagtagcaga atagtctgca
     1861 agaagacaat agaatttgaa aaagccaact tggttggctt ttttcagatt gctattggaa
     1921 ttttcagata attgtggtat agtagactca gtacacaaaa atgagtcccc actaacaaaa
     1981 actcaagatg aaacacatta acctgctcaa tttctaattg agacagtaat agggatggtc
     2041 ttgatttatg attggatata ggtacatttt ttacttctgg gtaaccgcta tcaaacgata
     2101 attgatagaa atatgttatt ctagagtagt tacataaagt cagttaatca aacattatat
     2161 gaacttgata atcgtaagga tattgaattg attgagcttg attagctggc acataaagga
     2221 ggaaaaagcg tggctaccga aaaaccgctt ttagacatta aagatttaca cgtcggtttc
     2281 cgtattggag atgattattt tgacgctgtt gacggtgttg acatctcatt gcaaaaaaat
     2341 gaaattttgg caattgtagg ggaatctggt tgtggtaaat caactttggc aacgaccatc
     2401 atgggcttgc ataacccttt gaataccaaa atcacaggaa gcatccagta caatgatatg
     2461 gaattgattg gtatggatga agctaagtac aatacggtac gtggtaatga tattggtatg
     2521 attttccaag atcccttggc ttctctgaat ccattgatga cgattggtgc acagattgat
     2581 gaggcgctgt tctatcacac tgatttggat gctaatgctc gtacagagcg agttttggaa
     2641 ctcttggctc aggtaggcat tccaaatcct aagcggacat ttaaacagta tccacatgag
     2701 ctttcaggtg gtatgcgtca gcgtatcatt attgcgatgg ccttgtcttg taaaccgcca
     2761 attattattg cggacgaacc tacaacagcc ttggatgtta caattcaagc acaaatcttg
     2821 gatctcttaa atgatattca agcggagaca ggttctggta ttattttgat tacccatgac
     2881 ttgggagtag ttgcagaaac tgctgatcgt gtggctgtca tgtacggtgg tcagtttgtc
     2941 gaagtggcgc cagtagaaga actctttacc aatccaaaac atccatatac acgttcgctc
     3001 ttgaagtcaa atcctcaatc aggtagcgaa ggaggcgatc ttcatgttat tgaaggtatt
     3061 gtgccgccta ttacaaaaat ggttcgtaag ggttgccgtt ttgctccacg tattccgtgg
     3121 attggagcag aggctcatga agagaatccg agcatgcacg aggtaggtcc aaatcacttt
     3181 gttcgttgta cctgccatga gacattctat tttgaagggg aggcctaata gtgggattta
     3241 ttgaagtaaa agatttaaaa gtccattatc caattcgtag tggcttcttt aaccgtgtga
     3301 cggatcatgt ctatgctgtc gatggcgtta atattgagtt tgaagaagga aaaacctatg
     3361 gtttggtagg tgagtcaggt tctgggaaat cgaccattgg taaggcaatt atcggtttag
     3421 agcgtgccac ttctggtcag attatctatg aggggcaaaa tgtaaccaac aaatcacgtg
     3481 gtaaaaaagg aaactttaat cgtgatgttc agatgatttt ccaagattct ctttcaagtt
     3541 ttaacccgaa aaaacgtatt ttagacatca ttgctgaacc aatccgtaac tttgaccgct
     3601 tatctccaga tgaagaaaag aagaaggttc ttcatctctt ggatacaatc ggtctaaatg
     3661 aagaagccct gattaaatat cctcatgaat tctcaggtgg tcagcgccaa cgtatcggtg
     3721 ttgctcgtgc cttggctagt aaccctcgtt tgattattgc tgacgaaccg gtttctgcct
     3781 tggatttgtc tgtgcaggca caggtcttga actatatgaa atgtatccaa gatgagttca
     3841 agcttagcta tctctttatt tcccatgacc tcggtgttgt acaacacatg tgtgatgaat
     3901 tgttcatcat gtatcgcgga cgtttcgtag agacaggaaa taagaacgat atttataata
     3961 atccacaaca tatttacact aaacgtttgt tgtctgcgat tccaacgatt gacccagtca
     4021 accgtttgaa gaataaagaa aaacgtttgg ctgctgaaaa agagtatcaa gaaatgcaag
     4081 gtcaatacta tgatgaaaac ggtcgtgtgt acgatttgag atccttctca gaaactcatc
     4141 aagtagccct tccagaagga ggtcagaact aagatgtgga aaacagtatt acgtcgttta
     4201 ctcatgatga ttccgcagat tattatcttg agcatcattg ctttctttgt tgcgaagatg
     4261 atgccggggg atccctttac aggattgatt gacccaaata tcgatccggc aatcatcgaa
     4321 caaaaacgga ttgcggcagg ttactatgat ccaatccctg ttcagtattt ccgttggatt
     4381 ggtaatcttt tgcaggggga ttttggtcaa agtatcctct tcaagcaacc tgttgttgat
     4441 gttatcatgc aacgtttaaa caacactatt tggttgtctt tattgacgat ggtgttgact
     4501 tatttgattg cccttccatt ggggatgatt gcaggtcgtt accaaaactc gcttgcagat
     4561 aaaatcattg gcgtgtataa cttcttgaca ttttcaaccc caactttcgt ttttgccatc
     4621 cttatgcttt ggatgtttgg ctttagtttg ggttggttcc caacgcgtgg gtcgattgac
     4681 ggtggtgtag aaggatttgc agccattctc agtcgcttac accacatgat tctgccagcg
     4741 attacaatgg caattttgtc aacaacagta actattcaat atcttcgtac ggggattatc
     4801 gatgcgaaga gtcaggacta tgttcgtaca gctcgtgcca aaggtgttcc tgagagagtt
     4861 gtttataacc gtcatatctt ccgaaattct atcttgccaa ttgcatcttt cttgggctat
     4921 gaattgacag gattgattgg tggttctatc ttcattgaaa atattttcac ttatccaggt
     4981 atcggtcagt tattctttaa ctctatttct agtcgtgact atagtgtcat cttggctttg
     5041 ttgttgatat ttggtatggg taccttgttg gggacattga tttcagatat tatcatgagt
     5101 atcgtggatc cacgtatccg tgtgaaatag aaggaggagt aaggtgagta aagaaaataa
     5161 aaaacaagta caatctgctt caactcctcc aggtggcttc agtgtcatcg cgcgggagtt
     5221 tatgaaggat cgacttgcat tgacctcatt aattatctta gtgacggttc tcttggcagt
     5281 ctttatcgga gcgcttctgt tggatcaaga acaagttatg aaagtcaacc ttttgggacg
     5341 ttacttggaa ccaggtgtta acggctatat cttggggaca gatgaaggtg gtaaagatat
     5401 ctttggtcag ctgattatcg gcgctcgtaa ctcgatcatt atcggtttta ccattacgat
     5461 tattacaagt attgtcggga tctctgtcgg tctgatttct ggttattatg gcggtcgttt
     5521 ggacggattc ctgatgcgta ttgtcgattt catcatgatt ttgccggtaa caatgttgat
     5581 cattgtgttc gtgacagtta tcaagaacta tacgatctat catttcattc tcatcatgag
     5641 tgccttttat tggacggcca aggctcgtct attccgtaca aggactttgt cagaggcaag
     5701 cttggattat gtcaatgcat caaaaacatt gggaacaagc gactttatga ttatgttccg
     5761 tgaaatttta ccaaacttaa gttcattgat tatcgttaac ttgaccttga actttgcggg
     5821 aaatatcggt attgagactt cgctgactta tcttggcttc ggtctaccat caacagtacc
     5881 aagtttgggt accttgattg ccaatgcccg caatgcagat attttggaaa acaagacatg
     5941 gatttggttg ccagcagcca tcttcattct tgtgatgatg ctctgtatca actatgtcgg
     6001 acaagctttc caacgtgcag cagatgctaa gcagcgttta ggttaattgt aaaaagggtt
     6061 cctttgggaa caatgtcatt aagttaagca actaagcagc taaagaaact attcccgtct
     6121 gagcaattga tgacaaaacg ggaatagttt ttgtatttag ggcgcacaaa aaggaaggtt
     6181 tttaacccaa ttttttaact attatgatat tctataagtg aaacttacgg gagcctggct
     6241 ttttatcttt ctttggaagg ttcgattggg tcgaataatg gataaatgtt tggcaatgta
     6301 ggtttctaat tggaaggaag taagttcttc tctaagaaat tttcgacagg cataaacagc
     6361 gtctgaaaaa caaattttat aagtctgttt taactttgat gttttaattg taacgtgtga
     6421 ggttagccat ttacaaacat taaa
//
LOCUS       DATUCG010000123         6306 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.125, whole genome shotgun sequence.
ACCESSION   DATUCG010000123 DATUCG010000000
VERSION     DATUCG010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6306
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.125"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(152..844)
                     /locus_tag="VCA20_001998"
     CDS             complement(152..844)
                     /locus_tag="VCA20_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HEP1835753.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIEFDLAFTSVLKRAIKTTNLALEAADQLWVPVEKSWRLNERHYGGLTGLNKAEAA
                     AEFGDEQVHIWRRSYDTLPPEMAKDHEHSAHTDRRYAHLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKQLSDDEIMDVEIPNFPPLVFELDE
                     NLNIVKEYYLEA"
     gene            1070..2254
                     /locus_tag="VCA20_001999"
     CDS             1070..2254
                     /locus_tag="VCA20_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEP1835754.1"
                     /translation="MKKVAIVSAYRSAIGSFGGSLKDIAIADLGAQVLETALASKNIP
                     ADSVDEVIFGNVLSAGQGQNIARQIAIRAGIPQTASAYAVNKVCGSGLKSVLLAAQSI
                     MLGDNDVVVAGGIEIMSQAPYLSKTSRFGSKFGHITLEDSMLTDGLTDAFNDYHMGIT
                     AENVAEHYQVSRAEQDAFTYSSQEKAAKAIAEGRFVDEIVPIRLKNRKGETIFATDEY
                     PRLTPIEKLATLRPSFKKDGTVTAANASGINDGCAVLVLMSDEKASELNIQPLAYIEA
                     YATSGLDPAYMGLGPITASQKALQKLNKTVEDIDLFELNEAFAAQSIPVVKQLGIDPA
                     KVNVNGGAIALGHPIGASGSRILVTLIHELIKQEKELGLCSLCIGGGQGISLIVSNAQ
                     TS"
     gene            2283..3452
                     /locus_tag="VCA20_002000"
     CDS             2283..3452
                     /locus_tag="VCA20_002000"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEP1835755.1"
                     /translation="MNIGIDKIGFAAPDYVLDLADLAQARNVDPNKFKIGLLQSEMAV
                     APVTQDIISLGAKAAEAILTEEDKQTIDMVIVGTESSVDQSKAAAVTIHGLLGIQPFA
                     RSFEMKEACYGATAGLSLAKSHIAQFPESKVLVIASDIAKYGVASGGEPTQGAGAVAM
                     LVTANPRILVLNNDNVCQTCDIYDFWRPNYDKYPRVDGKFSTEQYTDCLTTTFDYYQQ
                     KTGKTLNDFAAMCLHIPFSKQGLKGLQAIAQDEETLSRLTERFQEAIVYNKVVGNIYT
                     GSIFLSLLSLLENSRALETEDQILFYSYGSGAVCEIFSGQLVEGYRNHLQENRLEQLN
                     QRTKLSVKEYEQVFFEEITLDETGSSLDLPEDQSPFALIKVDNHKRIYRKYSRMN"
     gene            4045..5304
                     /locus_tag="VCA20_002001"
     CDS             4045..5304
                     /locus_tag="VCA20_002001"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEP1835756.1"
                     /translation="MSTFSGFYKKSRQERIDILRQNRSLSEDSLDVLYKDENLPEAIA
                     GKMAENHLGTFSLPFSVLPELLVDGQTYSVPMVTEEPSVVAAASFGAKIIAKSGGFTT
                     TIHNRIMIGQVALYDISDHSRATQAILDHKESILETANQAHPSIVKRGGGARELTVES
                     KDEFLIVYLQVDVQEAMGANILNNMLEAVKDDLEELSKGQALMGILSNYATESLVTAQ
                     CHIAIPNLATSSAIAQETAQKIALASKLAQVDPYRAATHNKGIFNGVDAVVIATGNDW
                     RAVEAGSHAYASRDGQYKGLSTWSIDGEHLVGSIILPLPIASVGGSIGLNPKVAVAFD
                     LLQQSKARQLASIIASVGLCQNFAALRALVTSGIQAGHMKLHAKSLALLAGAEEHEVD
                     QLAQLLRKEKHSNLETAQKLLEKMREH"
     gene            complement(5325..5792)
                     /locus_tag="VCA20_002002"
     CDS             complement(5325..5792)
                     /locus_tag="VCA20_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEP1835757.1"
                     /translation="MTIRKARQSDIPVLNELLQDILQVHHQVRPDIFKSAGQKFSEAE
                     LVALLENPDKPIFVFELEGQVVGHLFCELSTATGDVLEPVKTLFIDDLCVANSARGQR
                     IGEQLYEFALSYAKEQGCHNLTLDAWADNAGAVRFYERQGMKPQKFRMEQIIN"
BASE COUNT         1857 a         1425 c         1276 g         1748 t
ORIGIN      
        1 agtcctcaac acgcaaacat gcatgtagca tttttttact tgccataata aattcctcct
       61 accatttaat accaccagta taccatactc tctatgaaaa atccgatatt caaaactaaa
      121 aaagcctgag cgaactcagg ccttaaatga attatgcttc caagtagtac tctttcacaa
      181 tgttcaagtt ttcatccaat tcgaaaacaa gtggtgggaa gtttggaatt tccacatcca
      241 tgatttcgtc atcagacaat tgtttgatgt gttttacaag agcacggatt gagttaccgt
      301 gtgcacctac gaatacgttt ttaccgtctt tcaatgctgg agcaatttta tcttcccaga
      361 atggaagggc acgctcaagc gttactttca agttttctgc atctggaata actgaatcat
      421 caaggtgtgc atagcgacgg tcagtgtgtg ctgaatgctc atgatcttta gccatttctg
      481 gtggcaaagt atcataagaa cgacgccaga tgtgaacttg ttcatcacca aattcagcag
      541 ctgcttcagc tttgttcaag ccagtcaacc caccgtagtg acgttcgttc aaacgccatg
      601 atttttcaac tggtacccac aattgatctg cagcttcaag agccaagttt gtagtcttga
      661 tagcacgttt caatacagat gtgaaagcaa ggtcaaattc gatacctgct tctttgatca
      721 atttacctgc atcgattgct tgttgtgtac ctttttcaga caaatcaaca tcagcccaac
      781 cagtgaaaag gttagctttg ttccattcag actcaccatg gcgagcaaaa accaatttta
      841 ccatttatgg attctccttt aaattttgag gttcccctct tatacttctc tattttacac
      901 gaaaagataa aaaaatgcca gcctttccac aatctgtgta cgatttcatt actctttcat
      961 gtaaattcag tctctagacc tatctttctt cttagaaaaa atacaaaata atggtataat
     1021 agtaaggctg aacacgacag ttcaaaacga taccactata ggagttatca tgaaaaaagt
     1081 agctatcgtg tctgcctatc gttctgccat cggcagtttc ggaggtagtc tgaaagatat
     1141 tgcaatcgct gatttagggg cgcaagtcct cgaaacagcc ttagctagta aaaacatccc
     1201 tgcagacagc gttgatgaag ttatcttcgg aaatgttctt tcagcaggac aaggacaaaa
     1261 tattgcacgt caaattgcta ttcgtgcagg cattcctcag acagcctctg cctatgcagt
     1321 caataaagtc tgtggttccg gtctcaaatc agtcctcctg gctgcccagt caatcatgct
     1381 aggtgacaat gatgtagttg tggctggggg cattgaaatc atgagccagg caccttatct
     1441 atccaaaact agccggtttg gtagtaaatt tggtcatatt actctagaag attctatgct
     1501 gacagacggt ttaacagatg ccttcaacga ctatcacatg ggcattactg cagaaaatgt
     1561 tgctgaacac tatcaagtca gtcgcgcaga gcaagatgct tttacataca gtagccaaga
     1621 gaaggctgca aaagctattg ctgagggaag atttgttgac gaaattgttc ctattagact
     1681 aaaaaatcgg aaaggcgaaa ctatttttgc cacagatgag taccctcgtc tgacgccaat
     1741 tgaaaaattg gcaacacttc ggccatcttt taaaaaggat ggaaccgtca ccgcagccaa
     1801 tgcttctggc atcaacgacg gctgtgctgt cttggtactc atgtctgatg aaaaagccag
     1861 tgagctaaat atccagccac tcgcctatat tgaggcctat gcaacaagcg gtttagatcc
     1921 tgcctatatg gggttaggtc ctattactgc tagccaaaaa gcgctacaaa aactgaataa
     1981 aacagtcgag gatatcgatc tattcgaact caacgaggca tttgcagccc aatcaattcc
     2041 agttgtcaaa caactcggca ttgatcctgc aaaagtcaat gtcaatggtg gcgctatcgc
     2101 ccttggacat ccaatcggag ctagtggtag tcgaatccta gtcactctga tccacgaatt
     2161 aatcaagcaa gaaaaagagc ttggtctttg ctcactttgt atcggaggcg gtcaaggcat
     2221 ctccctaatc gtttccaatg cgcaaacaag ttaacctatc atttgtacac aaaggatata
     2281 ttatgaacat cggtattgat aaaatcggat ttgcggcacc cgattatgtt ttagatttgg
     2341 ctgatttagc ccaagcccgc aatgtggacc caaataaatt taagattggt cttcttcagt
     2401 ctgaaatggc tgtagctcct gtcactcaag atattatcag tcttggggca aaggctgccg
     2461 aagccattct gacagaagaa gataaacaaa caattgacat ggttattgtc ggtacagaat
     2521 ctagcgtaga tcaaagtaag gctgccgccg tcaccattca tggattactc ggtattcagc
     2581 ccttcgctcg ctccttcgag atgaaggagg cttgttacgg tgcaacggct ggactaagtc
     2641 ttgccaaaag ccatatcgct caattcccag aatcaaaagt cctagtcatt gctagtgata
     2701 ttgcaaaata tggagtcgct tctggcggtg aacctaccca aggtgctggt gcagttgcca
     2761 tgttggtaac cgcaaaccca cgaattttgg tcctcaacaa tgataatgtc tgccagacat
     2821 gtgacatcta cgacttctgg cgtcctaact acgacaaata tccacgtgtt gatggtaaat
     2881 tttcaactga acagtacacc gattgcttga cgacaacttt cgactactat caacaaaaaa
     2941 caggcaaaac cctcaacgat tttgctgcca tgtgcttaca tatccctttc tccaaacaag
     3001 gtcttaaagg attgcaagct attgctcagg atgaagaaac tcttagtcgc ttgacagaac
     3061 gtttccagga agctatcgtc tataataaag tcgtcggcaa catctacact ggctctatct
     3121 tcctctctct tctttccctt ttggaaaata gcagagcgtt agagacagag gatcaaatcc
     3181 tcttttatag ctatggtagc ggcgctgtct gtgaaatttt cagcggtcaa ctcgttgaag
     3241 gttatcgcaa ccacttacaa gaaaatcgtt tagaacaact caatcaacgt acaaaactca
     3301 gcgttaagga atacgaacaa gtcttctttg aagaaattac ccttgatgaa accggttcat
     3361 cccttgacct accagaagac caaagtccct ttgcccttat caaagtggat aatcacaaac
     3421 gtatctatcg taaatatagt cgaatgaatt aactttcaga caaggaactg aggtgtaggt
     3481 agctagaaca gcctaaaggc tgttctaggt tagaaataag tgagcgggaa agaactcgac
     3541 tggtcaaaaa aagagttcgt caacaagtct ttatttctag ttgttgagct gaaacagtct
     3601 attcccagac tgtttcactc ccacccccgc atagctccaa aggttcgggg aacctttgga
     3661 ggttggagat atagcgaacg aagttcgcaa cagtcgttaa gtcagagtaa tgactgttgg
     3721 aggttggaaa tgaagcgtac tctgttcgca tcagtcgtaa tggtcagatt tggggtgtaa
     3781 aacacgaatt gctgagattt gcttcgcaaa tcctatctcc aacctttaac agtccactgg
     3841 actgttaaac ccaatcaacc actgcgctga gatgttgaca cgaaccctga gaagcgaggt
     3901 tggtcttttt gcccagcctc actttcgcag agtacggcaa gccgaaagtg acgatgtatc
     3961 aaagctaatt caaatgacta taaaagccaa aacggcgccc tttggctctc accaaggggc
     4021 gctttcctat agaaagagga acctatgtca actttttccg gattttataa aaaatcacgc
     4081 caagagcgca ttgatattct ccgtcagaat cgttccctat ccgaagatag cttagacgtt
     4141 ctatacaaag acgaaaacct gccagaagca attgcgggaa agatggcaga aaatcatcta
     4201 ggaacgtttt ccttaccttt ttctgtactt ccagaacttc tagtggatgg acaaacttac
     4261 tctgtaccga tggttactga agaaccttct gtggttgcag ctgcatcttt cggagccaaa
     4321 atcatcgcta aatctggcgg ttttacaacg actattcata accgtatcat gattggacaa
     4381 gttgctctct acgatatttc ggaccatagc agagcgacac aggcgattct tgaccataag
     4441 gagagtattt tagaaactgc caatcaagca cacccttcca ttgtcaaacg agggggcggt
     4501 gcaagagaat tgactgttga gagtaaggat gagtttttga ttgtctatct tcaggttgat
     4561 gtacaagaag ccatgggagc caatatcctc aataacatgt tagaagctgt taaggatgac
     4621 ttagaagagc tcagcaaggg acaggctttg atgggaatcc tctccaacta tgcaacggag
     4681 tcactggtta ccgcccaatg ccatattgcc atccctaatc tagctaccag ctctgccatt
     4741 gctcaagaaa ctgctcaaaa aatagccctg gcaagcaaac tggcgcaagt ggacccttat
     4801 cgtgctgcga cacataacaa agggattttt aatggtgtag atgctgtcgt tatcgctaca
     4861 ggaaatgact ggcgggcagt cgaagccggg tcccatgctt atgccagccg tgatggacaa
     4921 tacaaggggc tttctacttg gtctattgat ggcgagcact tggtcggctc catcatccta
     4981 cctcttccga ttgcctccgt cggcggctcg atcgggctca atccaaaggt agcagtcgct
     5041 tttgaccttc tccaacaatc aaaggcacgc caattagctt ctatcatcgc ttctgtcggt
     5101 ctctgtcaga actttgctgc cctgcgtgct cttgttactt cagggattca ggctggtcac
     5161 atgaagctcc acgcaaaatc cctagcgcta cttgctggcg ccgaggagca tgaagtagac
     5221 caactagccc aactcctgcg gaaagaaaag cacagcaacc tagaaactgc tcaaaaatta
     5281 ttggaaaaaa tgagagaaca ttaaaaagag ggactccctc ttttttaatt tattatttgc
     5341 tccatacgga acttttgtgg tttcattcct tggcgttcat aaaaacggac agctcctgca
     5401 ttgtcagccc atgcatccaa ggtaagattg tggcaaccct gctccttagc ataggaaaga
     5461 gcaaattcat aaagctgctc gccaattctt tgaccacgcg cagaattggc tacacacaaa
     5521 tcatcaataa acaaggtttt aactggttct aaaacatcgc ctgttgcggt agataattca
     5581 caaaaaagat gcccaaccac ttgtccctct aactcaaaga caaaaatagg cttatctgga
     5641 ttttccaaaa gtgctactaa ttcagcctca gaaaattttt gacctgcact tttaaaaata
     5701 tctggtcgta cctggtggtg cacctgtaaa atatcttgaa gcaattcatt taataccgga
     5761 atatcagact gtctagcttt acggattgtc ataatactct ccttttgagt ttttattatt
     5821 atagcacatg ttcatttttt aacaaaatag gtctacagac cgatgaagaa tttgaaaatt
     5881 cgagtatact ataggtgtat cctaaaattt ttcataaatc tcctaaaaaa tcagctggcc
     5941 aaggctggtt ttttgttgtc ttaaaacgag aatgtgtagt gtagaaaact actaaagttt
     6001 ctctctcttt gacgcaaaat gcttttgctt catttccaac ctcccagagt cactcccctg
     6061 actctttgac gcaaaatgct tttgcttcat ttccaacctc ccagagtcac tcccctgact
     6121 ctgggagccc caaaacacat aaaacatact aagattagta gtgaagaaat ctccgacggg
     6181 agagagtact cactactttt tctttatgtt aaagtaaagg tgtcttgtta agtcgaaggg
     6241 cttttaggcg ctagacgtcg caataaaaac tggcaagaca cctgttttag gtagaatttt
     6301 atcaaa
//
LOCUS       DATUCG010000124         6224 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.126, whole genome shotgun sequence.
ACCESSION   DATUCG010000124 DATUCG010000000
VERSION     DATUCG010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6224
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.126"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            66..248
                     /locus_tag="VCA20_002003"
     CDS             66..248
                     /locus_tag="VCA20_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019320686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEP1835758.1"
                     /translation="MPFVRIDLFEGRTEEQKIALAREVTEVVSRNTNAPKEAIHVFIN
                     DMPEGTYYPQGEMKRK"
     gene            complement(665..952)
                     /locus_tag="VCA20_002004"
     CDS             complement(665..952)
                     /locus_tag="VCA20_002004"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835759.1"
                     /translation="MKAEEFWWIYEKCFRRYEGPGFSIHQTPLGGDCGYFMKGVYQDE
                     GGVWKVDETIERSSYPHTRSFDSEEACFLFLYQEAKSNLSYELAFGRLKKR"
     gene            complement(1006..2838)
                     /gene="lepA"
                     /locus_tag="VCA20_002005"
     CDS             complement(1006..2838)
                     /gene="lepA"
                     /locus_tag="VCA20_002005"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEP1835760.1"
                     /translation="MNLEDLKKRQEKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMDLERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEILPIINKIDLLAADPERVRQEIEDVI
                     GLDASEAVPTSAKAGIGIEEILEQIVEKVPAPTGDVAAPLQALIFDSVYDPYRGVILQ
                     VRIVNGVVKPGDTIQMMSNGKTFDVTEVGIFTPKAIGRDYLATGDVGYIAASIKTVAD
                     TRVGDTVTLAENPASEPLAGYKQMNPMVFAGIYPIDSNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERIEREFNIDLIMTAPSVVYHVNMTDGEMID
                     VANPSEFPDPTKIANIEEPYVKAQIMVPQEYVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYEISEYRSSKLVKMDILLNGDKVDALSFI
                     VHKEFAYERGKIIVDKLKKIIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKAIGSVEVPQEAFLSVLSMDEDEKK"
     gene            complement(2934..3347)
                     /gene="ndk"
                     /locus_tag="VCA20_002006"
     CDS             complement(2934..3347)
                     /gene="ndk"
                     /locus_tag="VCA20_002006"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002934976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEP1835761.1"
                     /translation="MERTFFIIKPDALKRGLAGQILSRIERRGFQIRDLKMVTATEEL
                     ISQHYEHLTDKPFFPQLVQYMTSGPMIAGIIEGPEVIKSWRDMMGATNPVNALPGTIR
                     GDFATAPVEGIVANVVHGSDSAEAAEREIGLWLGK"
     gene            complement(3370..3957)
                     /locus_tag="VCA20_002007"
     CDS             complement(3370..3957)
                     /locus_tag="VCA20_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002915299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEP1835762.1"
                     /translation="MEINTHNAEILLSAVSKAQYPGDDIPEIALAGRSNVGKSSFINT
                     LLGRKNLARTSGKPGKTQQLNFYNIDDKIRFVDVPGYGYAKVSKTERAKWGKMIEEYL
                     TTRENLRVAVSLVDMRHEPSADDVQMYDFLKYYEIPVIIVATKADKIPRGKWNKHESM
                     IKKKLDFDKNDQFVIFSSETRHGYDQAWDAILSNI"
     gene            complement(4013..5239)
                     /gene="clpX"
                     /locus_tag="VCA20_002008"
     CDS             complement(4013..5239)
                     /gene="clpX"
                     /locus_tag="VCA20_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEP1835763.1"
                     /translation="MAVKHTHELIYCSFCGKNQEEVKKIIAGNNVFICNECVELAQEI
                     IREELAEEVLTDLADTPKPQELLNILNNYVIGQDRAKRALAVAVYNHYKRINFQDSRD
                     ENDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATALTEAGYVGEDVENIL
                     LKLLQAADFNIDRAERGIIYVDEIDKIAKKGENVSITRDVSGEGVQQALLKIIEGTVA
                     SVPPQGGRKHPNQEMIHVDTKNILFIVGGAFDGIEEIVKQRLGEKIIGFGHNNRAIDE
                     KESYMQHIIADDIQKFGIIPELIGRLPVFAALDQLTTEDLVRILTEPKNALVKQYQTL
                     LSYDDVELDFDEDALLAIAEKAIERKTGARGLRSIIEETMLDVMFEVPGQDNVKRVRV
                     TKAAVDGKEKPLLETA"
     gene            complement(5261..5434)
                     /locus_tag="VCA20_002009"
     CDS             complement(5261..5434)
                     /locus_tag="VCA20_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835764.1"
                     /translation="MERSLFGLFTAFLCGICVLCAIQAFRKKRFGLAILFLLNAFTNL
                     VNSIHAIYGFLFR"
     gene            complement(5437..5949)
                     /locus_tag="VCA20_002010"
     CDS             complement(5437..5949)
                     /locus_tag="VCA20_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEP1835765.1"
                     /translation="MTKKIVAIWAQDENGLIGKGDRLPWSLPADLAHFKETTTGHTMV
                     MGRITFDGMGKRALPNRHTIVLTTDKTYQLESEHVTILHSVEDVLDWYNKQEQTLFVI
                     GGGQIFTAFAPYIETLIVTDIHGQFNGDVYFPKAFPMEKFQLQSANLRPKDEKNPYDF
                     TIKTYERRDG"
BASE COUNT         1779 a         1382 c         1170 g         1893 t
ORIGIN      
        1 tgaaaatatc cttggagaat cataaaaatt atggtacaat aaacgaaaaa taaaggagaa
       61 actatatgcc atttgtacgt attgatttat ttgaaggtcg cacagaggag caaaaaatcg
      121 ctcttgcccg tgaagtaact gaggtggtat cacgcaatac caatgcacca aaagaagcca
      181 ttcatgtctt tatcaacgat atgccagaag gaacctacta cccacaaggc gaaatgaaac
      241 gaaaataagc agaaacccac tcttgcacag tgatatactc tccctatagt ggacattgaa
      301 ataataaaag attaaacaac ggccttgtcc ctcgttcaaa gaggggcaag gccgttgttg
      361 tgttcttgaa aatgttcatt attgtaaaaa tagatgtagc catcaatatc tgaaaccaac
      421 tcctcaaagg tccctttata gtttctagtg aaaatttttg ttttttcact agaaactata
      481 aggggagtat atcaggatag tcgagaggtt ttttgcataa aaataatgtt cattttcgaa
      541 aactttttat atagaataga caaataacag aagactaaaa agagtggaaa gattttgcaa
      601 atcaaaaaag gaagctcgtc caaaatcagt cggttggaag tgagcgctcc cttctcaaaa
      661 cagtttatct tttctttagc cgtccaaatg ccaattcata gctcaaattt gatttagctt
      721 cttgataaag aaagagaaaa cacgcttcct cactatcaaa agaacgagta tgaggataac
      781 tgctacgttc tatggtctca tcaactttcc atacgccgcc ttcgtcttgg taaacaccct
      841 tcataaaata tccgcaatct ccacctaatg gagtttgatg aatactaaat cctgggccct
      901 catatcgacg aaaacacttc tcataaatcc accaaaactc ttctgctttc attagtttct
      961 acctcctaag tgcaagctca tgataaaaag gtacactgga cctttttatt tcttctcatc
     1021 ttcatccatg ctgagaacgc tgaggaaggc ttcttgtggg acttcgacag atccgatggc
     1081 cttcatccgt tttttaccag ctttttgctt ttctaggagt ttgcgtttac gggaaacgtc
     1141 accaccataa cacttggcca aaacgttctt ccgaagagcc ttgatgtcgc tacgggcaac
     1201 aattttctga ccgatagctg cttgtattgg tacttcaaac tgttgacgcg gaatgatttt
     1261 cttgagtttg tccacgataa ttttcccacg ttcgtaggca aattccttgt ggacaataaa
     1321 gctgagggca tcgaccttgt caccattgag gagaatatcc attttcacca acttggatga
     1381 acggtattca gaaatctcat agtcaaagct ggcataacca cgagtggacg acttaagctt
     1441 atcaaagaag tcaaagacaa tctcagccag tggaatttgg tagataacat tgacacggtt
     1501 atcgtcgatg taatccatgg ttacaaaatc accacgttta cgctgagcca attccataac
     1561 tgcaccaaca tactcctgcg gtaccatgat ttgtgcttta acgtaaggtt cttcaatgtt
     1621 ggcaatcttg gttgggtctg ggaactctga cggattggct acatcaatca tttctccgtc
     1681 tgtcatattg acatggtaaa ctaccgatgg agctgtcatg atgaggtcaa tgttaaactc
     1741 acgctcaatc cgttcctgaa taacatccat gtgcaggaga cctaagaagc cacaacggaa
     1801 accaaagccg agggcctgcg atgtttcagg ttcgaactgc aagctggcat cgttaagctg
     1861 gagtttttct aaagcttcac gcaagtcatt atacttattg gaatcaatcg gatagatacc
     1921 ggcaaagacc attgggttca tctgcttgta gcccgctagt ggctcactgg caggattttc
     1981 agccaaggtc accgtatcac cgacacgggt atctgcaacc gtcttgatag aagctgcgat
     2041 ataaccaaca tcacctgtcg ccaagtaatc acgaccgatt gcttttggtg tgaaaatccc
     2101 tacttccgtc acatcgaaag tcttaccatt gctcatcatc tgaatggtat caccaggttt
     2161 tactacacca ttgacgatac gaacttgcaa gataacgcca cgatagggat cgtaaaccga
     2221 gtcaaaaatc aaggcttgca gaggtgcagc aacatcacca gtcggtgctg gaaccttctc
     2281 cacgatctgc tccaagattt cctcaatacc aataccagcc ttagccgatg ttggcactgc
     2341 ttctgaggca tccaaaccaa tcacatcttc gatttcctga cgaacacgtt ctgggtctgc
     2401 tgctagcagg tcaatcttgt taatgattgg caagatttcc aaatcattat ccaaggctag
     2461 gtaaacgtta gccaaggtct gtgcttcaat cccttgagcc gcatccacca ctaaaatagc
     2521 cccctcacag gccgcaagag aacgcgatac ttcataggta aagtcaacgt gtcctggtgt
     2581 gtcaatcaag tggaaaatgt atgtctctcc atctttagct gtatagttga gttcgattgc
     2641 gttgagttta attgtgatac cacgctcgcg ttccaagtcc atactatcta gcaactgggc
     2701 ttgcatttca cgactggaaa ctgtttctgt cttctcaaga atacggtcgg ccaaggttga
     2761 tttaccatgg tcgatgtggg caataatgga gaaattacga attttctcct gtcgtttttt
     2821 caaatcttct aaattcatgt ttcttcctct acaagggtat tactcctcat tatatcagat
     2881 ttagtgataa ttttccataa gaaaaacacc tagaaaacta ggtgtctgct ctgctatttt
     2941 cccagccaaa gaccgatctc gcgttcggct gcttctgcac tatctgatcc atgaacgaca
     3001 tttgccacaa ttccctcaac gggcgctgtc gcaaaatctc cacgaatagt cccaggtagg
     3061 gcgtttactg gattggtagc tcccatcata tctcgccaag atttgataac ttctggccct
     3121 tctataatgc cagctatcat cggtccgctc gtcatatatt gtactaattg cgggaagaaa
     3181 ggtttatctg tcaaatgttc ataatgttga ctgattagct cttctgtcgc tgtaaccatt
     3241 tttaaatctc ggatttgaaa tcccctgcgt tcgatacgtg aaagaatttg tcctgcaagt
     3301 ccacgcttaa gagcatctgg tttgatgatg aagaaagtac gttccatgat ttttctcttg
     3361 aactgtttat tagatgttac ttaagatagc atcccatgcc tgatcgtatc cgtgacgagt
     3421 ctcagaagaa aagataacga attgatcatt tttatcaaaa tccaatttct ttttaatcat
     3481 cgattcgtgc ttattccact ttcctcgtgg gattttatca gccttcgttg ccacaataat
     3541 aacaggaatt tcataatatt tcaagaaatc atacatttga acatcatctg ctgatggctc
     3601 atgacgcatg tctactaaac tcaccgcaac acgtagattt tctcgtgtcg tcaaatactc
     3661 ttcgatcatt tttccccact tagcacgctc agttttagat actttagcat agccataacc
     3721 tggaacatct acaaatcgaa ttttatcatc tatattataa aaattgagtt gttgagtctt
     3781 tcctggtttt cctgacgtac gtgcaagatt ttttcttcct agaagagtat tgataaatga
     3841 ggactttcca acgttagatc gcccggccag ggcaatttct ggaatgtcat cgcccggata
     3901 ctgggctttt gaaacagcac tgagcaaaat ctcagcatta tgtgtgttga tttccatttt
     3961 atacctttca taaataaggg tagaaaacaa tcagtttcca ccctttattt ttttatgcag
     4021 tttctagtaa aggcttttcc ttaccgtcta ctgctgcttt tgttacacga acacgtttaa
     4081 cgttatcttg gcctggcacc tcaaacatga catccaacat cgtttcttca atgatggatc
     4141 gaagtccacg agcaccagtc ttacgttcaa ttgctttttc tgcaattgct aacaaggcgt
     4201 cctcatcaaa gtcaagctcc acgtcatcat aagataagag tgtctggtat tgttttacaa
     4261 gagcattctt cggttccgtc aaaatgcgta ccaaatcctc tgttgtcaat tggtccaaag
     4321 ctgcaaatac aggcaaacga ccaatcaact ctggaatgat accgaatttt tgaatatcgt
     4381 ctgcaatgat atgttgcatg tatgattctt tttcatcaat agcacgatta ttatgaccaa
     4441 aaccgatgat tttttcaccg agacgctgtt tgacgatttc ttcgatacca tcaaatgcac
     4501 cgccaacgat aaacaggata tttttggtgt ccacgtgaat catttcttgg tttggatgtt
     4561 tgcgtccgcc ttgtggtggt acgctggcca cagttccctc gataattttt aggagagctt
     4621 gctggacacc ttcaccagat acatcgcggg taatggacac attttcacct tttttggcaa
     4681 ttttatcaat ttcatccaca tagataattc ctctttctgc acggtcgatg ttaaagtctg
     4741 cagcctgcaa aagtttgagg aggatatttt ctacgtcttc accaacataa cctgcctcag
     4801 taagagcggt cgcatcagca atagcaaacg ggacattcaa acttttagcc aaggtctgag
     4861 ctaggaaggt ttttcctgaa ccagtcgggc caatcatgag gatatttgat ttttgcaaat
     4921 caacatcgtt ctcatcacgg ctatcttgga aattgatacg tttgtagtgg ttgtaaacgg
     4981 ctactgctaa agcacgtttt gcacgatcct gtccgatgac gtagttgttc aggatattga
     5041 gtaattcttg tggctttggc gtatcggcca agtctgtcaa aacttcttct gccagttcct
     5101 cacgaatgat ttcctgtgct agttccacgc actcattaca aatgaagaca ttattccctg
     5161 cgataatttt tttaacttct tcttgatttt ttccgcaaaa tgagcaatag attagctcat
     5221 gtgtatgctt aacagccatg tgtttcctct ttcatatctc ttatctaaat aaaaatccat
     5281 aaatggcgtg aatactattg accagattgg taaaagcatt cagtaaaaat aaaattgcta
     5341 aaccaaaacg tttcttccga aaggcttgaa tcgcacaaag aacacaaatc ccacagagaa
     5401 atgctgtaaa taaaccgaat aaacttctct ccatgcctaa ccatctcttc tttcataggt
     5461 ttttattgta aaatcatacg ggtttttctc gtcttttgga cgtaaattgg cgctttgtag
     5521 ctgaaatttc tccataggaa atgctttcgg aaaatacaca tcgccattaa attgaccgtg
     5581 aatatctgtc actattaatg tttcaatgta aggtgcaaag gcagtaaaaa tttgtccccc
     5641 accaatcaca aatagtgtct gctcctgttt gttataccaa tctaggacat cttccacact
     5701 gtgtaaaatg gtaacatgtt cactctctag ttgataggtt ttatccgttg tcaaaacgat
     5761 ggtgtgacga ttgggaagag cgcgtttacc catgccgtcg aaggttatac gtcccatcac
     5821 catcgtgtga cctgttgtcg tttctttgaa atgagctaag tcagctggca atgaccaagg
     5881 aagtctatca ccttttccaa tcaatccatt ttcatcttga gcccaaatgg caacaatctt
     5941 tttagtcatt ttctctcact ttccgtccta tcattctatc aaaaaaacat gaaaaatgca
     6001 cttaccaagt aggccaagcg caaattttta tcatgttgaa actatcaccg actccttcct
     6061 cagagatagg gaagattccg agattcgtag aatgtgaatc tcgctggtac tggaaagccc
     6121 ccctcatctt aaatcgctaa gtcaaatttg agttgcggtt taactggctt cgaaagtctg
     6181 agtatagact gttgaaggtt ggaaatagaa agaacgaagt tctt
//
LOCUS       DATUCG010000125         6219 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.127, whole genome shotgun sequence.
ACCESSION   DATUCG010000125 DATUCG010000000
VERSION     DATUCG010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6219
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.127"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(20..307)
                     /locus_tag="VCA20_002011"
                     /pseudo
     CDS             complement(20..307)
                     /locus_tag="VCA20_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000110.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
     gene            complement(321..2150)
                     /gene="glpO"
                     /locus_tag="VCA20_002012"
     CDS             complement(321..2150)
                     /gene="glpO"
                     /locus_tag="VCA20_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEP1835766.1"
                     /translation="MEFSKETRRLAIERMQDRQLDLLIIGGGITGAGVALQAAASGME
                     TALIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQNIAPHIPKPDP
                     MLLPVYDEPGSTFSLFRLKVAMDLYDLLAGVNNTDMANKVLTKEEVLEREPNLKQEGL
                     IGGGVYLDFRNNDARLVIENIKRAAKDGALIASRIKAEKFIKDDAGKVVGIVARDLLT
                     DCTFEIRARLVINTTGPWSDEVRNLGGEGSGVLQMRPTKGVHLVVDSSRLSVPQPTYF
                     DTGHADGRMVFVLPRENKTYFGTTDTDYTGDLANPMVTQEDVDYLLDIVNNRFPEANL
                     TLDDIESGWAGLRPLLSGNGASDYNGGNNGKLSGDSFNSLIETVKGYLNNEKTRDDVE
                     HNLPHLEGSVSEKHLDPSAVSRGSALDRDDNGLLTLAGGKITDFRKMAEGAMEKVADI
                     LKEEHGRSFKLINSKTYPVSGGELNPANVAEEIEHLAQLGVKKGLVYDDALYLANLYG
                     SNAPKVFALNHKVEAVSGLNKRDLLSLHYAMKEEMTLTAVDYLLRRTNYMLFMREQLD
                     AVAPDILKEMAAYYAWSEEEVAQQEQLLADTLVKNDLTYLKEK"
     gene            complement(2226..3740)
                     /gene="glpK"
                     /locus_tag="VCA20_002013"
     CDS             complement(2226..3740)
                     /gene="glpK"
                     /locus_tag="VCA20_002013"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEP1835767.1"
                     /translation="MSTEKYIMAIDQGTTSSRAIIFNKKGEKIGSYQKEFTQIFPKPG
                     WVEHNANEIWNSVQSVIAGSFIESGVRPDQIEGIGITNQRETTVVWDKETGLPIYNAV
                     VWQSRQTAHIADQLKADGHADMIHQKTGLVVDAYFSATKVRWILDQVPGAQERAEKGE
                     ILFGTIDTWLVWKLTDGKCHVTDYSNAARTMLYNIEELKWDDEILSLLNIPKAMLPEV
                     RSNSEVYGTTAPFHFYGAEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIVMNT
                     GEEMQLSQNNLLTTIGYGIGGKVYYALEGSIFIAGSAVQWLRDSMRMVTTSPESEELA
                     RKSTSNDEVYVVPAFTGLGAPYWNSDARGSVFGLTRGTTKEDFVKATLQSIAYQVRDV
                     IDTMQLDTGIDISVLKVDGGAAMNSLLMQFQADILGIEIARAQNLETTALGAAFLAGL
                     AVGFWKDLDELKELNAVGQSFQPTMNEARKEQLYKGWKKAVAATQLFAEVDEEQ"
     gene            complement(3968..5431)
                     /locus_tag="VCA20_002014"
                     /pseudo
     CDS             complement(3968..5431)
                     /locus_tag="VCA20_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775047.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(5421..6219)
                     /locus_tag="VCA20_002015"
                     /pseudo
     CDS             complement(5421..6219)
                     /locus_tag="VCA20_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735942.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
BASE COUNT         1769 a         1433 c         1211 g         1806 t
ORIGIN      
        1 tactggtttt gtttcattta actatacttc cgataaattg agcaaggata taagggacta
       61 cagaccccca tgggagattt cctgcaaggg ccatggcaat tgatacagct ggattgaggt
      121 gggcaggtcc caataaacca ctcacaaaag ctgccatggc tactgctaga ccccaaccaa
      181 ttgtgatgac aatccaacca gcatccttgg ccttactctt atctagtact actcctgcta
      241 ccacaccatt acctagtaag acaagtaagg aggttcccaa tacttcaccg attaattcat
      301 ttgtcatcat gcacctcttt ctatttttct ttcaaatagg tcaaatcatt cttaacaagt
      361 gtatctgcca acaattgttc ctgttgagca acttcttctt ctgaccatgc atagtaagcc
      421 gccatttctt tcaaaatatc tggtgctacc gcatccaact gctcacgcat aaagagcatg
      481 tagttggtac gacggagaag atagtcaact gcagtcaagg tcatctcttc cttcatagca
      541 taatgaaggg aaagcaaatc acgcttgttc aagcctgaaa cggcttctac tttatggttc
      601 aaggcaaaga cttttggcgc atttgagccg tacaagtttg ccaagtaaag cgcatcatca
      661 tagacaagac ctttcttcac acctagttga gccagatgtt cgatttcttc tgcaacgtta
      721 gccgggttta attcaccacc agagactggg taagtcttag aattgatgag tttgaagcta
      781 cgaccatgct cttctttgag gatgtctgca accttttcca ttgctccttc ggccatttta
      841 cggaagtccg tgatttttcc accagcaaga gtcagcaaac cattgtcatc acggtcaagg
      901 gcagagccac gtgatacagc tgaaggatcc aagtgttttt cagaaacgct gccttccaga
      961 tggggcaaat tgtgttccac atcgtcacga gtcttttcat tgttcaaata acctttgacc
     1021 gtttcaatca agctattaaa gctgtcacca ctgagcttac cgttgtttcc accattgtag
     1081 tcagaagcac catttccaga caagaggggg cgaagacctg cccaaccgct ctcaatgtca
     1141 tccaaggtca agtttgcttc tgggaagcga ttgttgacaa tatcaagcag gtagtcaaca
     1201 tcttcttggg ttaccattgg atttgccaaa tcaccagtat agtctgtatc tgtcgtacca
     1261 aaataggttt tattttcacg tggaaggaca aagaccattc gaccatccgc atggccagta
     1321 tcaaagtaag tcggctgtgg tacagacaag cgagagctat cgacaactag gtgcacaccc
     1381 ttggtcggac gcatctggag aacgcctgaa ccttctcccc ccagattacg cacttcatca
     1441 ctccatggtc ctgttgtatt gatgaccaaa cgcgcacgaa tttcaaatgt gcagtctgtc
     1501 agcaaatcac gagcgacgat accgacaact tttccagcat catctttgat gaatttctca
     1561 gctttgatac ggctggcaat caaggcgccg tctttggctg cacgtttgat attttcaata
     1621 accaagcggg catcattgtt acggaagtca agataaactc ctccaccaat caaaccttct
     1681 tgcttcaagt ttggttcgcg ttcaagtact tcttctttag tcaagacctt attggccata
     1741 tcggtattat tgacgccagc caagagatca tagaggtcca tagccacttt caagcggaag
     1801 aggctaaagg tagaacctgg ctcgtcgtag actggcaaga gcatcgggtc tggttttgga
     1861 atgtgagggg caatattttg tactacagca cgttcagata cggtatcgga taccacttcc
     1921 acgtcaaatt gtttgaggta gcgaagacca ccgtggacca atttggttga gcgacttgaa
     1981 gtgccttctg caaagtcttg catttcaatc aaggctgttt ccataccgct tgctgccgcc
     2041 tgtaaagcca cacctgcacc agtaatgcca cccccgataa tcaagaggtc aagttgacga
     2101 tcctgcattc gctcaatagc taagcgtctt gtttcttttg aaaattccat acgattcact
     2161 tcctaacttt cgaataggac cgtctagcaa gaggaaagcc tgctttcccc tcgctaacaa
     2221 gtcttttatt gctcttcatc cacttctgca aatagttgag ttgctgcgac agcttttttc
     2281 catcccttgt aaagttgttc tttacgagct tcgttcatgg ttggttggaa ggattggccg
     2341 acagcgttga gttctttcaa ttcatccaaa tctttccaga aaccaactgc taaaccagcc
     2401 aagaatgctg ctccaagagc tgtagtttca aggttttgag cgcgtgcgat ttcaataccc
     2461 aaaatatctg cttggaattg catgaggaga ctgttcattg ctgcaccacc gtcaaccttc
     2521 aagacagaaa tatcaatacc tgtatccaat tgcatggtat caataacatc acgaacttgg
     2581 taggcaatgg attgaagagt tgctttgacg aagtcttctt tagttgttcc acgagttaag
     2641 ccgaatactg agccacgcgc atctgagttc caatacggag ctccaagtcc tgtaaaggct
     2701 ggaacaacat agacttcatc attgctggta gacttacgag caagctcttc tgactcaggt
     2761 gatgtagtca ccattcgcat actgtcacgg agccattgaa ccgccgaacc agcgataaag
     2821 atagatcctt caagcgcata atagactttg ccgccaatac catagccaat tgttgtgaga
     2881 aggttgttct gtgacaactg catctcttca cctgtattca tcacgatgaa ggaaccagtt
     2941 ccgtaggtat ttttcaccat acctggctca aatgccaatt gtccaaatag agctgcctgt
     3001 tggtcacctg ccatgccaga gattggcact tctgcgccat agaaatggaa tggagctgtt
     3061 gtgccataaa cttcagagtt tgaacgaact tccggcaaca tggcttttgg aatattgaga
     3121 agagataaaa tttcatcatc ccatttcaac tcttcaatgt tgtagagcat ggtacgagct
     3181 gcgtttgaat aatccgtcac atggcatttc ccatctgtca atttccaaac caaccaagta
     3241 tcaatggtac cgaagagaat ctcacctttt tcagcacgtt cctgcgctcc agggacttga
     3301 tccaaaatcc aacgaacttt ggttgctgag aagtaggcgt cgactactag acctgtcttt
     3361 tgatgaatca tatctgcatg accatccgcc ttcagctggt cagcaatatg agctgtttga
     3421 cgagattgcc agacaacggc attgtagatt ggaagacctg tttccttatc ccagacaacg
     3481 gttgtttcac gctggttggt aataccaatt ccttcaatct gatcaggacg aacaccgctt
     3541 tcgataaagg accctgcaat cacagactga acagaattcc aaatttcatt ggcgttgtgt
     3601 tcaacccaac ctggtttagg gaaaatttgc gtaaactctt tttggtaact gccaatcttt
     3661 tcaccttttt tattgaagat aatggcacgt gaacttgtag ttccttggtc aatggccatg
     3721 atgtattttt cagtagacat aggtctccct cctgtttgtt tactttcgtt gaaaacgctt
     3781 ccgttttctt ggtaattcta ttctaaccca ttcttgtgaa tttttcctat cattttttca
     3841 aaaaaataaa aaaatctgat aaacagtggc tagaaagatg attctatcaa ctgttatcag
     3901 agtttataaa ataaattttt caaggttaaa tgcacttaca tttttcacaa agactatctg
     3961 acatcatcta gcgatgctcg ataggaaaag aagttttggt cgccataatc tctgattgac
     4021 aggacttttg atgatatata tcctgtatcc atgcctttaa ttgtacgatg tcttgtattt
     4081 tcaatcgatt tcctaagcaa taaactggaa tgcttgtatt ttcgaggtaa tctgtaatga
     4141 tcaaatcata gtcatatcct gcttgataag gctcaataag aacctgacga ttgccttcta
     4201 acttctccct tagttgcaac aagagaggat aggatagaac ctcatgtagg gaagaagcaa
     4261 aagcaacctt gacctgtata ggctggacct ggctaaaata aatatacaaa tccgcaatat
     4321 ttatcagata agagttcctt atttctttat cgacttttct atgaaaaact gatttataga
     4381 cgtcttttag caagtcttct gcttctgaat agaaactaga agcttctacc tcttccagtt
     4441 caacggaaga ataaaagaaa tatagctggc tcatgatttg gttcaaatga tagagcgctt
     4501 cttctgagac tggtaaattt ccctcaaaat ataaccgtaa tttctgaaga gccaaagagg
     4561 tagcttccat aatcggtcca ccgaaaccaa gaacctgctc taattgatgc ggcgctaaaa
     4621 tgaaatggct aaacaggaaa gcaaacaacg ccataatctc cccttcttag aaagaggtgg
     4681 attgttgctg acttagtgta aaatacatct ttctcaaacg tcggtaaaac ttatgttctt
     4741 gataaggttc catcaatttg taaaggtgct taaaatctag ctgtcgcaat cgagagcgtt
     4801 gctgtgaaat cattaaccac aacaaaatac gatgggcttg cttaggattg agcggcgatt
     4861 gatagaagcg ttcgaaaacc ggcaataagg cctctatttt ccctagcata aactcttctt
     4921 gtaattccgc tctcgtagta gtcaaactca acatttgatg taaaaagtac cgtatttgaa
     4981 gctcactacc ttttagacga ccacttttga ttccaatacc aaattctgcc aatatctggt
     5041 tcaaagaggc aagatggcga ttcagagtcg cttcgcttat cagcaattct tgagccagct
     5101 gttgaataga aacctcttcc ttatcagata aataaacaat aatttgatat ttggtactgg
     5161 tttgacagag ataggctagg atacgatgaa gactcaaact gaccttttta ttcaaaaaga
     5221 ccttttcatc ttcgagttga aaagagagtc ccaagtcggt tacttctgct tcttcattga
     5281 aagatacaat gtagcgaaga agagttgatt ttgacaattc taaatagtga caaatatcct
     5341 ttagcgctgg cgcctccttc ctatcttcta agtagaggag aagctgataa atggcgcgct
     5401 cacgcttttc caataaggaa tcaatccgca tagacaccct ccccatcaac ctgataaaat
     5461 tgccgaatat actcttgagc ggcctctacg atgaagggga ccagaggagt atttccagga
     5521 agacagagcc aagaatgttc taccaagtca aagacggtca aatcctttga aatcgctaga
     5581 gacagtacat ccatctgttc aatataatct gcagtagaaa cgagttgccc ccctatgatg
     5641 cgcccactcg tttcttcgac aatcagcttg aaatctacta aagttggttc ccactggcta
     5701 tacgaccttc taatccgaat cgatttactc tccaaccaca agctcgtctc actctcggtc
     5761 aagccaaggc tagttatata atagccaaat acatgactag agacaacccg ctgggctctc
     5821 ttaataggag caatcctctt ttgcaaattt cgagcagcca tcctccctgt cataatcgca
     5881 tgatgaatca tcggaagata tgcctgtcca aaataatcta ctggagactg accaaatcac
     5941 ctacagcaaa aatattctct tgacttgttt caagaaactc atccacccat acagtccctt
     6001 ctgcagttac ttttagcctg tctttcacta gctgaatatt gggcgtcaaa ttggtggatg
     6061 gcaagactaa atcagctgga taattacctg tgactgtatg acaaaccagc tgttcttcct
     6121 gagtatcaat cgttatctga ttgactgttt cggataaatg aagttggatt cctctttttt
     6181 ccatctcttg acgaatcgac gcaatcatat cctcatcaa
//
LOCUS       DATUCG010000126         6104 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.128, whole genome shotgun sequence.
ACCESSION   DATUCG010000126 DATUCG010000000
VERSION     DATUCG010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6104
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.128"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            <1..1714
                     /locus_tag="VCA20_002016"
     CDS             <1..1714
                     /locus_tag="VCA20_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEP1835768.1"
                     /translation="YPEVQQMIEDTYNRLYNRTVSKTYDGSHLTIDGLAQNISLRPHQ
                     KNAIQRIVEEKRALLAHEVGSGKTLTMLGAGFKLKELGMVHKPLYVVPSSLTAQFGQE
                     IMKFFPTKKVYVTTKKDFAKAKRKQFVSRIITGDYDAIVIGDSQFEKIPMSREKQVTY
                     INDKLEQLREIKLGSDSDYTVKEAERSIKGLEHQLEELQKLERDTFIEFENLGIDFLF
                     VDEAHHFKNIRPITGLGNVAGITNTTSKKNVDMEMKVRQVQAEHGDRNVVFATGTPVS
                     NSISELFTMMNYIQPDVLERYQVSNFDSWVGAFGNIENSMELAPTGDKYQPKKRFKKF
                     VNLPELMRIYKETADIQTSDMLDLPVPEAKIIAVESELTQAQKYYLEELVERSDAIKS
                     GSVDPSRDNMLKITGEARKLAIDMRLIDPAYTLSDNQKILQVVDNVERIYRDGAEDKA
                     TQMIFSDIGTPKSKEEGFDVYNELKDLLVDRGIPKEGIAFVHDANTDEKKNSLSRKVN
                     SGEVRILMASTEKGGTGLNVQSRMKAVHHLDVPWRPSDVGRFEVVRRKALKTGLSQKI
                     LMQA"
     gene            1795..2004
                     /locus_tag="VCA20_002017"
     CDS             1795..2004
                     /locus_tag="VCA20_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007156672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP1835769.1"
                     /translation="MALSYKPLWHLLVEKKMNKEDLKRAANITSNIVSRMSKDTYVNL
                     DSLEKICLALDCTLNDVVEIVKDTE"
     gene            complement(2041..2847)
                     /locus_tag="VCA20_002018"
     CDS             complement(2041..2847)
                     /locus_tag="VCA20_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012858488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP1835770.1"
                     /translation="MKCKITLQERLKDLRVAHNLNLEELAKLTGISKSALGNYENDDY
                     KEINHGNLVILAQFYKVSTDYLLCLTENKNHPNTDLTTLHLSDDMIDLLVSGSINNRL
                     LCEIATHDKFKELMADTEIFVDGVATKRFNDLNSSLETVRAEILNQNPNVSNDHTLRT
                     LSAAQVCEEDFFCHITHKTWDSIIKDIRKNHEQDIDSIPEDDNTLSLQKIRQIMISSG
                     SNIDKFIEIFCNSFQLKYKRLSEDEKEFLRKLFKKSPIIKNSGINFRRKK"
     gene            3292..3675
                     /locus_tag="VCA20_002019"
     CDS             3292..3675
                     /locus_tag="VCA20_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEP1835771.1"
                     /translation="MELSKGELTVMETLWDGECLDENGEIQALELYKLLNEKYGFAKT
                     SCYTFFGRLLEKGAITRRYPKYTLKPIVSREEALKEQQEEAIQRLFQGSIINVCRAFL
                     SEKQVTKKELQEMRDLIDSFDIEEK"
     gene            3680..5050
                     /locus_tag="VCA20_002020"
     CDS             3680..5050
                     /locus_tag="VCA20_002020"
                     /inference="COORDINATES: protein motif:HMM:NF017390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HEP1835772.1"
                     /translation="MIELVAVSALERSIKSIFIIALILLVRRKLNIKSIKCANIILWS
                     ILFIYLIFPYSFLIKIENPSRYGILQYILEPVLIISEYIRAFVKDFGYLLSKVNRVFI
                     ASLLLIYVITQIIQRNKAMKNSIPIENDKRVIEVINLFKLKRKIDVLISDKVKVPITY
                     GVARPKIILQSHILEDDELLRYVLIHELTHIKKFDIVFNHIKNLIACVYWYNIFILIA
                     SRYMEDDIEVLCDKLVIQRVGDTVNNRKEYCISMLKLIEQKEYTKRVVLKLHPTKERM
                     VIMKKWKRTFSGVCAFALVTALSFTAFADVKAVEKEQVLSSEISSNVKLNDNNRVVEI
                     TDEEYNRLTLGEIPFNELRVADVDSKVTLDGLEHKSYKFNMESWTEPNHDGFTVKMSE
                     MSCSGGLNYALIIKENGNEIYNSNFTKATTLTVKAYHNSRYEVIIQNRSTNSLKYRVK
                     INSYIR"
     gene            5219..5818
                     /locus_tag="VCA20_002021"
     CDS             5219..5818
                     /locus_tag="VCA20_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003778694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835773.1"
                     /translation="MMKRKRSIRLASILMLGLIVSTSVPVNAATLKPAVGGTSALAEE
                     NQYDGIALRTAQGKKLTIPGGKGTLTSNAWRSTSATSSGNTYQWDYQVSAVYSGNKTV
                     ESIKTSWYGGASLRNSGSISLGVSNSGATVGASSSWQYITTPVKYWENTNGAKSSDYR
                     SNLVVSPSRDYRSGTISLTNTARVKLKGDPKPYEVSAGV"
     gene            5885..>6104
                     /locus_tag="VCA20_002022"
     CDS             5885..>6104
                     /locus_tag="VCA20_002022"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835774.1"
                     /translation="MIKKIGFIFICLVLVAFSGCNKKIIGNNEFFYNPYTQKMIKYNS
                     ELKNAELWSSTRNQFQYAVGNENIYVDGN"
BASE COUNT         2204 a          871 c         1205 g         1824 t
ORIGIN      
        1 gtatccagaa gtccaacaga tgattgaaga cacctataat aggctctaca atcgtacggt
       61 atcaaagacc tatgatggta gccatttaac cattgatgga cttgctcaga atatctcctt
      121 acgtcctcac caaaagaatg ccattcaacg aattgtggag gaaaaacgtg ctctactagc
      181 ccacgaggta gggtctggaa aaaccttgac catgcttggg gctggcttca agttgaaaga
      241 attagggatg gtgcataaac ctctctatgt ggttccttct agtttgactg cccagtttgg
      301 tcaagaaatc atgaaatttt tccctaccaa gaaagtctat gtgactacta agaaagactt
      361 tgccaaagcc aaacgcaagc agtttgtgtc ccgtattatt acaggggact atgatgccat
      421 tgtcattggg gattcacaat ttgagaagat accgatgagt cgtgaaaaac aggtcaccta
      481 tatcaatgac aaacttgagc aactccgaga gatcaagcta ggaagtgaca gtgattatac
      541 agtgaaagaa gcggagcgtt cgattaaggg actagaacac cagttggaag aactccaaaa
      601 actagagcga gataccttta tcgaatttga aaatcttggc attgattttc tctttgtgga
      661 tgaggctcat cacttcaaga atatccgtcc aattactgga cttggaaatg tcgctggcat
      721 taccaacacc acttcaaaaa agaacgtaga tatggagatg aaggtgagac aggttcaggc
      781 agagcatgga gatagaaatg ttgtttttgc gacaggaaca ccagtttcta actctattag
      841 tgaacttttc accatgatga attacattca acctgatgtt ttagagcgat accaggtatc
      901 caattttgat tcgtgggttg gggcttttgg gaatattgaa aactctatgg aactagcccc
      961 gacaggagat aagtaccaac ccaagaagcg gttcaagaaa tttgtcaacc ttcctgaact
     1021 catgcgaatc tacaaggaaa ctgccgatat tcagacctca gatatgctcg acttacctgt
     1081 acctgaagct aagattattg cggtggaaag cgagttaacg caagctcaga aatactattt
     1141 agaagagctg gtagagcgtt cggacgctat caagtcaggt agtgttgatc caagtagaga
     1201 taacatgctt aaaatcacag gagaagccag aaaactagct attgatatgc ggttgattga
     1261 ccctgcctat actttatcgg ataatcagaa aatccttcaa gtagtcgata atgtcgaacg
     1321 aatctaccgt gatggagctg aagacaaagc cactcagatg attttctcag atattggaac
     1381 ccctaaaagt aaggaagaag ggtttgatgt ctacaatgaa cttaaggact tgttggtcga
     1441 tcgagggata ccaaaagaag gaattgcctt tgtccatgat gccaatactg atgagaagaa
     1501 aaactctctc tcacgaaagg tcaatagcgg agaagtacgg attctcatgg cttcgactga
     1561 aaaaggtgga acgggtctaa acgtgcaatc acgcatgaaa gctgttcacc atttagatgt
     1621 tccatggcgt ccctcagatg ttggacggtt tgaagtggta aggcgaaaag cattgaaaac
     1681 aggactatct caaaaaatac taatgcaggc ataactaata agcccataca gaggattttt
     1741 acagtggttt gtgttataat aacaaatata aaactactgt aaaggaggta aactatggct
     1801 cttagctata aacccttatg gcacttatta gttgaaaaaa agatgaataa agaggacttg
     1861 aaaagggctg ccaatataac aagtaatata gtttcaagaa tgagcaagga tacttatgtc
     1921 aatcttgatt ctttagaaaa aatatgttta gcgttagact gtaccctcaa tgatgtagtt
     1981 gaaatcgtaa aggatacaga ataacaactg taaaatcact taattatata ttaggtggtt
     2041 ttattttttt cgcctaaagt taataccgga gtttttaatg ataggagatt tcttaaatag
     2101 tttccttaaa aattcctttt catcttcaga taatctttta tattttagtt gaaaagaatt
     2161 acagaaaatc tcaataaatt tgtctatatt acttccggaa gaaatcataa tttgacgaat
     2221 tttctgtaat gataaggtgt tatcgtcttc aggaatactg tcaatatcct gttcatgatt
     2281 tttgcgaata tcttttataa tgctatccca tgttttatgt gtaatatgac aaaagaaatc
     2341 ttcttcgcat acttgagcag ctgatagagt tctcaaagta tggtcattag atacattggg
     2401 attctgattt aggatttcag ctcttactgt ttcaagagaa gaattcaagt cattaaatcg
     2461 tttagtagca acaccgtcaa caaaaatctc cgtatcagcc ataagttcct taaacttatc
     2521 atgtgtagca atttcacaaa gtagacggtt attaatagaa ccgcttacta atagatcaat
     2581 catatcatca ctcaagtgta gagtagttaa atctgtattt gggtgatttt tattttccgt
     2641 aaggcaaagt aaatagtcgg tagaaacctt ataaaactgt gcaagtataa ccaagttccc
     2701 atgattgatt tctttgtaat catcattctc atagtttcct aaagcagatt ttgaaatacc
     2761 tgttagttta gctagttctt ctaaatttag attatgtgca actcttaaat cctttaagcg
     2821 ttcctgtaag gttattttac acttcataac agataacctc cttgatacaa aatattctta
     2881 taaatattat accataccat ttccacaatc gtggaaaagt gggaacaaaa acgaatattc
     2941 caagatttag gacatacggg aataggaaca aaaatacaat attatacaga cataaagaaa
     3001 attgttcttt gacaattgaa tgagcggtct gtaaggatat gtacctatgg ggaagtaggc
     3061 gatgactttc taagtatcag aaaagagctt gccaagtaga acaaaacgtc gctgtgagat
     3121 tcgggggtag gaactatatc aggcgtagcc gacaaaaata tagaaagtaa gaaatagggc
     3181 ttgcaaagta ataaaatata tgatatactc ataatgaaaa tataaataac aaagaaaata
     3241 aaatgctttg taagaataca acaaataaag tgaactagtg aggtggttaa aatggaatta
     3301 tcgaagggag agcttacagt gatggaaacg ctgtgggacg gagaatgttt agatgaaaat
     3361 ggagaaatac aagcattaga attgtacaaa ctgctaaacg aaaagtatgg ttttgcaaag
     3421 acttcttgtt atactttttt cggaagattg cttgaaaaag gagcaattac aagacgttat
     3481 cccaaatata ctttaaaacc gattgtttca agagaagaag cattaaagga acaacaggaa
     3541 gaagctatac aaagattgtt tcaaggatca ataatcaatg tatgtagagc gtttttaagt
     3601 gaaaagcaag taacgaaaaa agaattacaa gaaatgcgag atttaattga tagcttcgat
     3661 atagaggaaa agtaatggga tgatagaatt agttgcagtt tcagcattag aaagaagcat
     3721 taaaagtatt ttcataatcg cccttatact tttggttaga agaaaactaa atatcaaaag
     3781 tataaagtgt gcaaatatta ttttatggtc aatactattt atctatttga tttttccata
     3841 ttcatttttg ataaagattg aaaatccgag tcggtatggg atactacaat atattttaga
     3901 acctgtattg ataataagtg aatatattcg agcatttgta aaagattttg gatatctttt
     3961 atcaaaagtt aatagagtgt ttattgcaag cttattgctc atttatgtga taacacaaat
     4021 tattcagaga aataaggcaa tgaaaaattc tattccgata gaaaatgata agagagtaat
     4081 agaagttatt aatttattca agttgaaaag gaaaatagat gtacttataa gtgacaaggt
     4141 aaaagttcca ataacctatg gagttgcccg acctaagata atactacaaa gccatatatt
     4201 agaagatgat gaattattga gatatgtgct aatccatgag cttacacaca ttaaaaaatt
     4261 tgatattgtg tttaatcaca ttaaaaattt aattgcttgt gtgtattggt ataacatatt
     4321 tatccttatt gcctcaagat atatggaaga tgatattgaa gtcctttgtg ataaattagt
     4381 tattcaaaga gttggagata ctgttaataa tagaaaagaa tattgtatat ctatgctgaa
     4441 attgatagaa caaaaagaat atacaaagcg tgtagtatta aagttacacc ctacgaaaga
     4501 aaggatggtt attatgaaaa aatggaaaag aacattttca ggagtgtgtg cttttgcatt
     4561 agtaacagct ttatctttta cagcatttgc tgatgtaaaa gctgttgaga aagaacaagt
     4621 gctttcaagt gaaatttctt caaatgtgaa acttaatgat aataatagag ttgtagaaat
     4681 aactgatgaa gagtacaacc gtttaacact tggagaaata ccgtttaatg aattaagagt
     4741 tgctgatgta gatagcaaag taacattaga tggtttagaa cataagtctt ataaatttaa
     4801 catggagtca tggacagaac cgaatcatga cggatttaca gttaagatga gtgaaatgtc
     4861 atgtagtgga ggattaaatt acgctttgat tataaaagag aatggaaatg aaatttataa
     4921 ttcaaatttc actaaagcta ctactttaac tgtcaaagca tatcacaata gtagatatga
     4981 ggttattatc cagaaccgtt caacaaattc attgaaatat agagttaaga taaatagcta
     5041 tataagatga ttttgatata cattttttgg agcgaaagga gggaaatagt atgaaatata
     5101 tgaatcctca gaggtataag aatggagata gtgctttttt aaagaatata ttttttattt
     5161 gatgaaagct ggatgttgag gtactaacca ctacaattta tatattagga ggagaataat
     5221 gatgaaaaga aaaagatcta taagattggc atcgatttta atgttaggat tgattgtatc
     5281 aactagtgta cctgtgaatg cagcaacatt gaaacctgct gttgggggta catctgcgtt
     5341 ggcagaagaa aatcaatatg atggaatagc acttagaact gcacaaggaa aaaagcttac
     5401 tataccagga ggaaaaggaa ccttaacttc caatgcatgg cgttcaacga gtgctacatc
     5461 ttctggaaat acttatcagt gggactatca ggtttcagct gtatatagcg gaaataaaac
     5521 agtcgaaagc ataaaaactt cttggtatgg aggagcttct ttgagaaatt caggttctat
     5581 aagtttaggt gtaagtaatt ctggagcaac agttggagca agttcaagtt ggcaatatat
     5641 aactactcct gttaaatact gggaaaatac taatggtgca aagagttcgg attatagaag
     5701 taatttagtt gtaagcccaa gtagagatta tcgttccggt acaataagtt taactaatac
     5761 tgctagagtg aaattgaagg gtgatccgaa accatatgaa gtttcagctg gagtataaat
     5821 ccaattttgt ataggactcg attaatcgag tcctatactt attttataac gaaaggtggt
     5881 tattatgata aaaaaaatag gatttatatt tatctgttta gtattagttg cattctcagg
     5941 gtgtaataaa aaaataatag gaaataatga atttttttat aatccatata ctcagaaaat
     6001 gataaaatac aattctgaat tgaaaaatgc tgaattatgg agttctacta gaaatcagtt
     6061 tcagtatgct gttggaaacg aaaatatata cgttgatgga aatt
//
LOCUS       DATUCG010000127         6075 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.129, whole genome shotgun sequence.
ACCESSION   DATUCG010000127 DATUCG010000000
VERSION     DATUCG010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6075
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.129"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            <1..503
                     /locus_tag="VCA20_002023"
     CDS             <1..503
                     /locus_tag="VCA20_002023"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835775.1"
                     /translation="KKIQKKFERTLKNDLKIWETAETVSVAKTKAKVFNFSDLEKAYK
                     LNENYLAKILELDVIGLQEQRQYNDWIAEQKTIVSKKQFDKMNETIKYANKQYNQKYK
                     VSLEEQNNVMLKALFERFFTSLDVKRWENDRIIVANTTDVTDLNYLSAQQRLNQQDGR
                     AYFQEK"
     gene            complement(697..1005)
                     /locus_tag="VCA20_002024"
     CDS             complement(697..1005)
                     /locus_tag="VCA20_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000217346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835776.1"
                     /translation="MTVLNIRISSEEKEEIKTRAEKEGLSVSSYARNLLFLEYNAKDI
                     QDNTVVIQEKDERISDLKNQIDDYKKQIEQLHTIILATQKDNQLLIELKSKKWWQFWK
                     "
     gene            complement(1127..1423)
                     /locus_tag="VCA20_002025"
     CDS             complement(1127..1423)
                     /locus_tag="VCA20_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835777.1"
                     /translation="MIRNKKKAFIQKTNEILSDPELKLSKELKALLVESIDLCEKGEK
                     ISYLSYKIYPWVLEELTLNQGQSDKLKMFKRYLEQQRWKYYFGSALGMAFVSIR"
     gene            complement(1438..1842)
                     /locus_tag="VCA20_002026"
     CDS             complement(1438..1842)
                     /locus_tag="VCA20_002026"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835778.1"
                     /translation="MKKIILLFVALVSLFVFQNNVSADTLDDNIPQMTLSEALKSGEK
                     EVIITEIDIPEAAKVLNSIRMRMPRSAGGDWVYMYTAEPYAFYRHSKTKKWKMVQVTP
                     TWTHVQNTVVNGWISSMAGGYDYATSFGQRKP"
     gene            complement(1977..3308)
                     /locus_tag="VCA20_002027"
     CDS             complement(1977..3308)
                     /locus_tag="VCA20_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partition protein"
                     /protein_id="HEP1835779.1"
                     /translation="MAITYKKNARNIFVIPFYARFWFWWWLIVVFSALCSTICYFVPS
                     KLCQIGIIGLWSLVAILTIWRAYSLTKSVSKAETIGKYITQRKAEKAVTRSLLATMSV
                     NRLQDTPYISVPNVAVCDSRPSHISVEVEKLAGMYDIEKMTEDINASFRGKLGGYAVT
                     SGIITTDGLTYKFVLEDVATDKTWRPATIKDVTAEKYAIKLQEGLTVRLDERAHIAVW
                     GKTGSKKTTVLLGMILQLFSMGADIRFIDGKDEFSSFQGFYPAEKIVSDPDDVFEQLE
                     DVLAIIKQRQQIMADEVQKRGKMGLKASEVGLCPVVIVADEIGSIVALMDSKQAKRFV
                     ADLVAIIQRGRSVGVSVIASTQDPSTDTLPQKIRQQFSTKILLGSANSDIQRMAFGEV
                     ATDGDVEDFRGFFTCDGLTNQPMKFFVCDLYSHGLNDLEVFRKAYKIGFFV"
     gene            complement(3310..4458)
                     /locus_tag="VCA20_002028"
     CDS             complement(3310..4458)
                     /locus_tag="VCA20_002028"
                     /inference="COORDINATES: protein motif:HMM:NF014537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEP1835780.1"
                     /translation="MNDFSYKIDWLSFSLGEQYDMLDCHRFQVLKLLGYEIDDFQSIS
                     GRFFYNAGLTLGNYVNVYYNEHDIEKIKGSTDTVTYVFTGQGCTDLFEKIGGDWAGLF
                     TSLLSINANITRLDVALDDFVGLLKFPTILSKLNLGHYKSPKKAHNIVRSSDVNRNEK
                     GLTIYIGSSRSSSSKGNYYCRMYDKKAQYLEKHQQIPEEIENADFWQRYEISYTKKKS
                     QKIAELLAAGEPVDKIYKQSMGKLVTFLVPTKNKDGQLYENKSDWKVCAWWSKFLETN
                     EKIGFENPERDIDLGGLLRWLQVSVMPNIKNLERIGYRFGFDIYKMMKEFPIDEFSKK
                     QERLYNNLNTKKVSDDDNSNVAQALRKQNQRLTKEVERAFQRFKDKGY"
     gene            complement(4565..4738)
                     /locus_tag="VCA20_002029"
     CDS             complement(4565..4738)
                     /locus_tag="VCA20_002029"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835781.1"
                     /translation="MYIIDLYSEMLSEHKKICEGRSDICKENSLECLKFVFEKNKDIL
                     EKHFDYPSISRPV"
     gene            complement(4740..5114)
                     /locus_tag="VCA20_002030"
     CDS             complement(4740..5114)
                     /locus_tag="VCA20_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835782.1"
                     /translation="MSQLQKLQALKSQAKAQAKVSNMTNFENIVGIYLGTEPTLHFPK
                     LFDENGNKVKDEKGNDKRSKTSDGWTYTFAEFQTCKKIKIVLQKQFDFKLLSAYNISG
                     EGYDIKSADMYFIEKDITVANY"
     gene            complement(5621..5827)
                     /locus_tag="VCA20_002031"
     CDS             complement(5621..5827)
                     /locus_tag="VCA20_002031"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835783.1"
                     /translation="MIKFIDELILQDLSFDEIIHEFNLFARSNDSDVYSNFTFEDLNV
                     RDEFESYLNNSSNIDDLRKKWLSR"
BASE COUNT         1978 a         1192 c          823 g         2082 t
ORIGIN      
        1 aaaaaaaaat tcaaaaaaaa tttgaacgta cactcaaaaa tgatttaaaa atttgggaaa
       61 ctgctgaaac tgtatctgtt gcaaaaacga aagctaaagt tttcaatttc tcagacctag
      121 aaaaagcgta taaactcaac gagaattatt tagccaaaat tttagagtta gacgtgattg
      181 gattacaaga acagcgtcaa tataacgact ggattgctga acaaaaaaca atcgtctcaa
      241 aaaaacaatt tgataaaatg aatgaaacta tcaaatatgc taataaacaa tacaatcaaa
      301 aatataaagt tagtttagaa gaacaaaata acgtaatgtt gaaagcgttg tttgaaagat
      361 ttttcacttc attagatgtt aaaagatggg aaaatgatag aattatagta gcaaatacaa
      421 cagatgttac agacctaaat tatttatcag ctcaacaacg attaaatcaa caagacggaa
      481 gggcatattt tcaagaaaaa taaaaaagcg gtcatttttt gatcgctttt ttatttcatc
      541 tgaaaatcat cttctaattt gtctaatacc aactcatgaa ttttttcaag aaaatcatca
      601 gcttgtctaa ttgtctttgt ttgacatgcg acatattcag caatttcttc aactaatttt
      661 gtcatgtgat ttcctcctca catatctatt cgcaaatcat ttccaaaact gccaccattt
      721 tttagacttt aattctatca atagttgatt atctttctga gttgctagaa taattgtatg
      781 caactgctca atctgtttct tataatcatc aatttgattt tttaaatctg aaatacgttc
      841 atctttttct tgtattacaa ctgtattatc ttgtatatct tttgcattat attctaaaaa
      901 tagcagatta cgagcatacg agctaacaga gagaccttct ttttcagctc tagtctttat
      961 ttcttctttt tcttccgaac tgatacgaat atttaaaaca gtcatttttt cactcatagc
     1021 acaccctcta taatacattt tataacacaa tgatatcaca aaataatata gaaattagaa
     1081 taaaagccct agacagggct tttattctaa atgcaattaa aattcactac ctaatagaaa
     1141 caaacgccat tcctaaagca gaaccaaaat aatatttcca cctctgctgt tctaaataac
     1201 gtttgaacat ttttaattta tcagattgac cttgattcaa agttaattct tccaataccc
     1261 atggataaat tttgtaggaa agataactta ttttctctcc tttttcacac aaatcaatcg
     1321 attctactag caaagctttc aattctttgc ttagctttaa ttctggatca gataatattt
     1381 catttgtttt ttgtataaat gctttttttt tattcctaat cattacattt accattttta
     1441 aggttttctt tgaccaaaac tagttgcata gtcataacct ccagccatag aagatatcca
     1501 accatttaca acagtatttt gaacatgtgt ccatgttgga gttacttgaa ccatcttcca
     1561 ctttttagtc tttgaatgac gataaaacgc atatggttcc gcagtataca tatataccca
     1621 atctccgcct gctgaacgtg gcattctcat tcgaattgaa ttcaacactt ttgcagcttc
     1681 tggaatatct atttctgtaa ttataacttc cttttctcct gatttcaaag cttctgacaa
     1741 agtcatttga ggaatattat cgtctaacgt atcagctgat acgttattct gaaaaacaaa
     1801 taaactaacc aaagcaacaa ataacaatat aatttttttc ataaaaaacc tcctttattg
     1861 atattattat atgatatagt tacaacaata tcaacccttt cctgactcac ctccaaaatg
     1921 tccaccatct cttagatttt tcctctatca ataattgatt ctttatgtca acttatttat
     1981 acaaaaaagc ctattttata agcttttcta aacacttcta aatcattgag tccatggcta
     2041 tataaatcac aaacaaaaaa cttcattggt tgattagtta aaccatcaca agtaaaaaaa
     2101 cctcgaaagt cttccacatc gccatccgtc gcaacctctc cgaaagccat tctctgaatg
     2161 tcactatttg cagagcctag cagaatttta gtgctgaatt gttgtcggat tttctgcggt
     2221 aatgtatcag ttgaggggtc ttgtgtgcta gctatcacgg acacacccac agaccgacca
     2281 cgttggataa tagctacaag gtcagctaca aacctcttag cttgcttact gtccatgaga
     2341 gcaacgatag agcctatttc atcagccaca atcacaacag ggcagagacc cacttcagag
     2401 gctttcagac ccattttacc acgtttctgc acttcatcag ccattatctg ctgacgttgc
     2461 ttgatgattg ccaaaacgtc ctctaattgc tcgaaaacgt catcagggtc agaaacaatc
     2521 ttttcagctg gatagaaacc ttgaaacgaa ctgaattcat cttttccgtc aataaatcga
     2581 atatctgccc ccatgctgaa caactgcaat atcatgccga gaagtaccgt ggtcttcttg
     2641 ctcccagttt tgccccaaac tgcaatatgt gcccgttcgt ctaatcttac tgttagcccc
     2701 tcttgtagct ttatagcgta tttttcagcg gttacgtcct ttatagtcgc tggtcgccac
     2761 gtcttgtcag ttgccacgtc ctcaagtaca aatttgtagg tcaagccgtc agttgttatt
     2821 attccagacg tgactgcata accgcctagc ttcccacgaa agctggcatt gatgtcttct
     2881 gtcatcttct cgatgtcata cataccagct aatttctcga cttctacgct gatgtgtgac
     2941 ggtctgctat cgcataccgc cacgtttgga acagagatat aaggggtatc ttgcaatcga
     3001 ttgacggaca tagtcgcaag caggctccta gtaactgcct tttctgcttt ccgttgtgtg
     3061 atatattttc cgattgtctc cgctttgctc actgactttg tcagtgaata cgctcgccaa
     3121 atcgtcaaaa tagcgaccag cgaccacaag ccaattatgc caatttggca taatttagac
     3181 ggtacaaaat agcaaatagt tgaacataat gcagaaaata caacaatcaa ccaccaccaa
     3241 aaccaaaatc tagcgtaaaa aggaattaca aagatatttc tagcgttctt tttataggtt
     3301 attgccattt taatacccct tatctttgaa acgctgaaat gctcgttcta cttctttagt
     3361 gagtcgctga ttttgttttc ttaacgcttg tgcaacatta ctattatcat catctgaaac
     3421 ctttttagta ttcagattat tataaagtct ttcctgtttc tttgaaaatt catcaatagg
     3481 aaattctttc atcatcttgt aaatatcaaa accgaaacga taaccaatac gctctaaatt
     3541 tttaatattt ggcattactg aaacttgtag ccacctaagc aaaccgccta aatcaatatc
     3601 acgttctgga ttttcaaatc cgattttctc atttgtttct agaaattttg accaccaagc
     3661 acaaactttc caatcgcttt tattttcgta aagctgacca tctttgtttt tggtcggcac
     3721 tagaaaagtg actaacttac ccatcgattg cttataaatt ttatccactg gctctccagc
     3781 tgctaacaat tcagcaatct tttgcgattt tttcttagtg taactaattt cataacgttg
     3841 ccaaaaatca gcgttttcaa tttcttcagg tatttgttga tgtttctcta aatactgcgc
     3901 tttcttatcg tacatacgac aataataatt gcctttcgat gatgaactgc gagaagaacc
     3961 gatgtaaatt gttagacctt tttcattacg attaacatca gaagacctca caatattatg
     4021 tgcctttttc ggtgatttat aatgccctaa attcaatttt gatagaattg taggaaattt
     4081 caataaccca acaaaatcat ctaatgcgac atctagacgt gtaatgttag catttatact
     4141 caacaatgaa gtaaataatc cagcccaatc tccacctatt ttttcaaaca aatcagtaca
     4201 accttggcca gtaaaaacat aagtaactgt atctgttgaa ccttttattt tttctatatc
     4261 atgctcatta taataaacat taacataatt ccccaaagtc aaaccagcat tataaaaaaa
     4321 tcgtccacta atagattgaa aatcatcaat ttcataccct aataacttca acacttgaaa
     4381 tctatgacaa tctaacatat catattgttc cccaagagag aaagaaagcc agtctatttt
     4441 atagctaaaa tcattcaatt tacgttaaac ctcacaatcc ctttaacacc aagcctttta
     4501 gtctagtttt tcatatttct agacccccta attagtgtag ggggtcttcc tgtttgagct
     4561 acgctcaaac aggtctagaa attgaaggat agtcaaaatg cttttctaaa atatctttat
     4621 ttttttcaaa aacaaattta agacattcta aactattttc tttgcaaata tcagaacgac
     4681 cctcacatat ttttttgtgt tcagacaaca tttctgaata taaatcaatg atatacatat
     4741 caataatttg caacagttat atctttttca ataaaataca tgtctgccga cttaatatca
     4801 tatccttctc cgctgatgtt gtatgcggaa agtagcttaa aatcaaattg tttttgtaaa
     4861 acaattttga tttttttaca tgtttgaaat tcagcaaatg tatatgtcca gccatcagac
     4921 gtttttgaac gtttatcgtt tcctttttcg tccttgactt tatttccgtt ttcatcaaat
     4981 aatttaggga aatgcaaagt tggttctgtt ccaagataaa ttccaacaat attttcaaag
     5041 tttgtcatat ttgaaacttt tgcttgtgct ttagcttgtg atttcaaagc ttgaagtttt
     5101 tgaagttggc tcatcccaac tgattgattt tgtgtatttg acataataaa aatgcccttt
     5161 ctaaaaatgt tttcattgct taccacaacc tacaaaacat cttaagaaaa gacaccaact
     5221 ttttatataa ccatacctct tgacccactc agggataatt tggtataatt gtcttgttcg
     5281 aaaaaagatg ggtcttacga gtagcttgca ggcgttagta agactttttt tatttatctg
     5341 ttagttgttc gaagactaac agatattttt attttacatc aattaaaaga agatgtaaac
     5401 ccttaatttt tcatgggctc tgcccaaacc cgccagacgg atggccgtct ggactcccag
     5461 cctctgaaag aaaagatttt caatttttct ttgtcgactt actcaatcta gcagaatatc
     5521 aatctcatca agagtcaaga tgaacgaact tcgttcgctc ttgattctcg attgatattc
     5581 tgctctttag attgagtcga cgaaagaaaa attttaaaaa tcatcgacta agccattttt
     5641 ttcgcaaatc atcaatattt gacgaattat ttagataact ttcaaattca tcacgaacat
     5701 ttaaatcttc aaatgtaaaa tttgaataaa catcagaatc atttgaacga gcaaataaat
     5761 taaattcatg aataatttca tcaaatgata aatcttgcaa aatcaattca tcaataaatt
     5821 ttatcatata gatattcctt tctttatttt ttgtatctta ataatatcag atttcgaaat
     5881 acatgtcaaa cgttttttat gtttttttct tatctgtaca gcttatttct gttgcttttg
     5941 tttgtgtttc gtgatacaat aacgagaaaa acaaaataca aaaaaaggag taagaaatgg
     6001 aaaatcaaat cacacttaaa aattatatag aacatctaac tacattattg gatttgccga
     6061 ctacagacaa tgttc
//
LOCUS       DATUCG010000128         6056 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.130, whole genome shotgun sequence.
ACCESSION   DATUCG010000128 DATUCG010000000
VERSION     DATUCG010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6056
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.130"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(<1..261)
                     /locus_tag="VCA20_002032"
     CDS             complement(<1..261)
                     /locus_tag="VCA20_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEP1835784.1"
                     /translation="MKKILVLHTGGTISMQANHQGEVAASKTNPMTQIDSPLEETQVT
                     SLDFLNVPSPHIRLEHMMTLYQKIKEEQMNFDGFVITHGTDTL"
     gene            331..1176
                     /locus_tag="VCA20_002033"
     CDS             331..1176
                     /locus_tag="VCA20_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEP1835785.1"
                     /translation="MTIKAVFFDIDGTLLTDNRMVSSSTILAINALKEKGILVGLATG
                     RDPRFVLQYMASLGLDLAIAYNGQYIFSREEVIYSQSLEPKQIEQIMEYAQTHHKDLS
                     FGTAKGIFGSKIMSAGTGNFAYRVTRMIPESWAGIINFIFNRLVRWISPQQETNLKGF
                     LFQPIYQLMLLTTERETQSLEMLFPDLSFTRSSPYATDIISKGNSKLSGIVKVVDRYG
                     FELDEVMVFGDSNNDFEMLNEIQHSVAMGNGTKKVKQAASFVTDTNNRDGIYKALIHF
                     GVIEG"
     gene            1178..1711
                     /locus_tag="VCA20_002034"
     CDS             1178..1711
                     /locus_tag="VCA20_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEP1835786.1"
                     /translation="MFQSKDDNFNRVKDFHFLMDGETQELPSVYDGQMALHRAGFKLE
                     ELVEFLHAASESEVEFHDFIQQLHQDLDTAAAKVSGKRGFGVSMQDQVDALLDILYFT
                     YGSFVLMGVDPEPIFQIVHTANMGKTFPDGKAYFDPITHKILKPDDWEERFAPEEKIQ
                     EELKRQMKREWERTRPR"
     gene            complement(1860..2312)
                     /locus_tag="VCA20_002035"
     CDS             complement(1860..2312)
                     /locus_tag="VCA20_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015445117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEP1835787.1"
                     /translation="MTQSYKTILVAVDGSKGAELALHKAIHVALRNQARLIIAHVIDT
                     RALHNVVAFDASVYESLEREAELLLEEYKQEALNAGLTDVQIRIEFGNPKTLLAINIP
                     KETGADLMLLGATGLNAFERLLIGSSSEYIMRHATIDLLIVRDGEKSL"
     gene            2467..3681
                     /locus_tag="VCA20_002036"
     CDS             2467..3681
                     /locus_tag="VCA20_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEP1835788.1"
                     /translation="MKQFNKSTKLDDVAYDIRGPVLEEAMRMRANGEQILRLNTGNPA
                     EFGFTAPDEVIRDLIHNARKSEGYSNSKGIFSARKAIMQYCQLKKFPNVDIEDIYLGN
                     GVSELIVMSMQGLLDNGDEVLVPMPDYPLWTAAVSLAGGKAVHYVCDEAAEWYPDLAD
                     MESKVTSRTKAIVLINPNNPTGALYPKEILEGIIDIARRHELIIFSDEIYDRMVFDGA
                     VHIPIATLAPDLFVVTMNGLSKSHRICGFRVGWMILSGPKKHVKGYIEGLNMLSNMRL
                     CSNVLAQQVVQTSLGGYQSVDELLMPGGRLYEQREFITNAINDIPGLSAVKPKAGLYV
                     FPKIDREMYRVEDDEQFVLDFLKQEKVLLVHGRGFNWKDPDHFRIVYLPRVDELAEIQ
                     EKMSRFLRQYRR"
     gene            3833..4621
                     /gene="codY"
                     /locus_tag="VCA20_002037"
     CDS             3833..4621
                     /gene="codY"
                     /locus_tag="VCA20_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEP1835789.1"
                     /translation="MTTLLEKTRNITSILKRSEEQLAEELPYNAIAEHLSAIIDCNSC
                     IINSEGEVLGYHMNYETNNDRVEEFFQNKQFPEGYVKAVAQVYDTQVNLPVESELTAI
                     PVESRSTYPNGLTTIAPIHVTGIRFGSLIIWRNDEQFHDDDLILVEIAATVVGIQLLN
                     FQREEDEKNIRRRAAVNMAVNTLSYSEMKAVAAILGELDGNEGQLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVLIPAIFDEIKKRDY"
     gene            4623..5174
                     /locus_tag="VCA20_002038"
     CDS             4623..5174
                     /locus_tag="VCA20_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002268947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEP1835790.1"
                     /translation="MTKALISIDYTIDFVADEGKLTAGKSAQAISERIAQVTQEAFEN
                     GDYIFFAIDGHEEGDEFHPEAQLFPSHNIIGTQGRDLYGPLADFYQKHKGYARVRWMD
                     KRHYSAFSGTDLDVRLRERGVDTVVLTGVLSDICVLHTAIDAYNKGYRIEVVSSAIAA
                     LTEENHQFALNHLRHVLGATIID"
     gene            5456..5803
                     /gene="rplS"
                     /locus_tag="VCA20_002039"
     CDS             5456..5803
                     /gene="rplS"
                     /locus_tag="VCA20_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEP1835791.1"
                     /translation="MNPLIQSLTEGQLRTDIPSFRPGDTVRVHAKVVEGNRERIQIFE
                     GVVISRKGQGISEMYTVRKISGGVGVERTFPIHTPRVDKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            complement(5920..>6056)
                     /locus_tag="VCA20_002040"
     CDS             complement(5920..>6056)
                     /locus_tag="VCA20_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEP1835792.1"
                     /translation="KLEATNKLIKDIKRQAFGFRNFKTKIYIALNIKNERTNFVLSRC
                     "
BASE COUNT         1715 a          977 c         1439 g         1925 t
ORIGIN      
        1 taaagtatct gtgccatgtg taatgacaaa accatcgaag ttcatttgtt cttctttgat
       61 tttttggtat agagtcatca tgtgttctaa gcgaatgtgt ggacttggaa cattgagaaa
      121 atccaaggat gtcacttgag tttcttctag tgggctatca atttgggtca ttggattggt
      181 tttactcgct gcaacttcac cctgatgatt ggcttgcata gaaatggttc cacctgtatg
      241 cagtactaga atttttttca taaattttca gtttctttca aagtgtgata tactaattgt
      301 aacataattc ttagaaagta ggtgagttgg ttgacgataa aggctgtatt tttcgatatt
      361 gatggtacct tattgaccga caatcgtatg gttagtagtt caactattct cgccattaat
      421 gctttgaaag agaaggggat tttagttggg ctagctacag gacgtgaccc tcgttttgtt
      481 ttgcagtata tggctagttt agggctagat ttggccattg cttataatgg tcaatatatt
      541 ttttcaagag aagaagttat ttatagtcag agtttagagc caaagcaaat tgaacaaatt
      601 atggagtatg cgcaaactca ccataaggat ttatcttttg gaactgctaa aggcatattt
      661 ggtagtaaga ttatgagtgc tggaacagga aattttgcat atcgtgtgac gcgtatgatt
      721 ccggagtcct gggctggaat cataaatttt attttcaatc gtttggtacg ttggattagt
      781 cctcaacagg aaacgaattt gaaaggtttt ctctttcaac caatctatca gctgatgttg
      841 ctgacgacgg aaagggagac acaatcattg gagatgctct ttccggactt gtcttttacg
      901 cgttcgagtc cctatgcgac tgatattatc agtaagggaa attcaaaatt atcgggtatt
      961 gtaaaggttg tagatcgata tggttttgag ttagatgaag ttatggtatt cggggattct
     1021 aataatgatt ttgaaatgtt gaatgaaatt cagcactcgg tagcgatggg aaatgggacg
     1081 aaaaaggtaa aacaagcagc ttcctttgtt acggatacaa ataatcgtga tgggatttat
     1141 aaggcattaa ttcattttgg tgtgattgag ggataagatg tttcagagca aggacgataa
     1201 ttttaatagg gtaaaggatt ttcatttttt aatggatggt gaaacccaag aacttcccag
     1261 tgtgtatgat ggacagatgg ctttgcatcg cgctggtttt aaattggagg agttggtaga
     1321 atttctacat gcagcttcag aatctgaagt tgagtttcat gattttattc aacaacttca
     1381 tcaggattta gatactgctg ctgctaaggt atctggcaag agaggtttcg gggtttcgat
     1441 gcaggatcag gtagatgctt tgctggatat tctttatttt acatatggtt cgtttgtttt
     1501 gatgggggtt gatccagaac caatttttca gatagtccat actgcaaata tggggaaaac
     1561 atttccagat gggaaggcct attttgatcc aattacccat aaaattttaa aacctgatga
     1621 ttgggaagaa cggtttgcgc cggaggaaaa gattcaggaa gagctgaaaa gacagatgaa
     1681 aagggagtgg gaaagaactc gaccaagata aaaagagttc gtcttctcac ccccgcacag
     1741 ttgattaggc cagatttgga gtgcaaaaca cgaacaaatc tgccaatcaa ccactgcgcg
     1801 gagatgttga cacgaactct gagtagtggt cctgggcttt ttgcccagcc tcttttggtt
     1861 tataaacttt tttccccatc gcgaacaatg agtaaatcga tagttgcgtg gcgcatgata
     1921 tattcggatg aggatccgat taggagacgt tcaaaggcat ttagaccagt tgctccaagt
     1981 aacataagat ccgcaccggt ttcctttgga atattgatag ccagtagggt tttagggtta
     2041 ccgaattcaa tgcgaatctg tacatcggtc agaccggcgt tgagagcttc ttgtttgtat
     2101 tcttcgagga ggagctccgc ctctctttct aaagattcat agacagaggc gtcaaatgca
     2161 acaacattgt ggagagcacg ggtatcaatc acatgggcga tgatcagacg tgcttggttg
     2221 cgtagtgcga catgaatggc tttatgaaga gctaattctg ctcctttaga gccgtctaca
     2281 gcgacgagta ttgttttgta agattgtgtc ataataatca ttcctttcag aagagctttg
     2341 tcccgaaata ggtagagtag ttccttacat ttttattata agccttttga gagaaaatgt
     2401 aaaggattct tgtacttttt tagcttttac agtacaatag aaaagataga aagttgaggt
     2461 gacgacatga agcaatttaa caagtcaaca aaattggatg atgtagcata tgatattcgt
     2521 gggcctgttt tggaagaggc catgcgcatg cgtgccaatg gagagcagat tttacgtttg
     2581 aatacgggga atcctgcaga atttggtttt actgctccag atgaagttat tcgtgacttg
     2641 attcataacg cccgtaaatc agagggatat tcgaacagta agggaatttt ttctgcacgt
     2701 aaggctatca tgcagtattg tcagctaaag aaatttccaa atgttgacat tgaagatatt
     2761 tatcttggaa acggcgtgag tgagctgatt gttatgtcta tgcaaggttt actagataat
     2821 ggtgatgagg tattggtgcc aatgccagac tatcctctct ggactgccgc agttagtttg
     2881 gctggtggta aagctgttca ctatgtttgt gatgaagcag ctgaatggta tccagatttg
     2941 gcagatatgg agtccaaggt gacttctcga accaaggcaa ttgtccttat caatccaaat
     3001 aatccaactg gtgctctcta tccgaaagaa attttagaag gaatcattga tattgctcgt
     3061 agacatgaat tgatcatttt ctctgatgag atttatgatc gtatggtctt tgacggagct
     3121 gttcatatcc ctattgctac cctggctcca gatttatttg ttgtgacgat gaatggactg
     3181 tctaaatcgc atcgtatttg tggtttccga gtaggctgga tgatcttatc tggtccgaaa
     3241 aagcatgtaa aaggttacat tgaaggcttg aatatgttgt ccaatatgcg tttgtgttca
     3301 aacgttttgg ctcaacaggt tgttcaaact tctttgggag gttaccaatc ggttgatgaa
     3361 ctcttgatgc ctggcggacg cttatatgaa caaagggagt tcattaccaa tgcgattaat
     3421 gatattccag gcctttcggc tgtaaaacct aaggctggtc tgtatgtctt cccaaaaatt
     3481 gatcgtgaga tgtacagagt tgaggatgat gaacaatttg tattggattt cctcaagcaa
     3541 gaaaaagtac ttttggtcca tggtcgaggt tttaactgga aggatccaga tcatttccgt
     3601 attgtgtatt taccacgtgt ggatgagttg gcagaaattc aagaaaaaat gtcacgattc
     3661 ttgcggcaat atcgtaggta atggaatttt gggctgaatg ttaatcagcc ttttcttttt
     3721 acttgaatga aattgtttgt atttttctaa caacggtaaa tattctgata attgttgaaa
     3781 ttttatgtat tttttgctat actgaaagca attacagagt aaagaggaaa atatgacaac
     3841 attattagag aagacacgga atattacttc tattttgaag cgttccgaag agcaattggc
     3901 agaagaattg ccttacaatg ccattgctga gcatttatca gctattattg actgcaactc
     3961 gtgcatcatt aatagtgaag gtgaagtttt gggataccac atgaactatg agacgaacaa
     4021 tgatcgtgtg gaagaatttt ttcaaaataa acaattccca gaaggatatg taaaagcagt
     4081 tgcgcaggtt tacgatacgc aggttaattt gcctgtcgag agcgagttga ctgccatccc
     4141 tgtcgaatca agatcgactt atccaaatgg gctgacaacg atagcgccta tccacgtaac
     4201 ggggattcgt tttggttcgc ttattatttg gcggaatgat gagcagtttc acgatgatga
     4261 tttgattttg gttgagattg cggcaacagt agttggtatt cagttgctta atttccaacg
     4321 ggaagaagac gagaagaata tccgtcgtcg tgcggcagtt aatatggcgg taaatacgct
     4381 atcttactca gaaatgaagg cagttgcagc tattttgggt gaattggatg gcaatgaggg
     4441 gcaattgact gcttctgtga ttgcagatcg tatcggtatt acacgctcgg tgattgtgaa
     4501 tgcactgcgt aagttggaga gtgcagggat tattgaaagt cgttctttgg gaatgaaggg
     4561 gacttatttg aaagttctta tcccagctat ttttgatgaa attaagaaac gtgactacta
     4621 aaatgacaaa agcattgatt tcgattgatt atacaataga ttttgtggca gatgaaggaa
     4681 agttgactgc tggaaaatca gctcaggcta tttctgaacg aattgctcag gttacgcaag
     4741 aagctttcga aaatggagac tatattttct ttgcgattga tggtcatgag gagggggatg
     4801 aatttcatcc tgaagctcag cttttcccaa gtcataatat cattgggacg caagggcgtg
     4861 acttgtatgg tcctttagca gatttttatc aaaaacataa agggtatgcg cgtgttcgtt
     4921 ggatggataa acgtcattat tctgcttttt ctgggacgga tttggatgtt cgactaagag
     4981 agcgtggtgt agatacggtt gtcttgacgg gtgttttgtc tgatatttgt gttcttcata
     5041 cagctattga tgcttataat aaggggtatc gtattgaggt ggtctcatca gccattgctg
     5101 cattgacgga ggagaatcat cagtttgctc ttaaccattt gcgtcatgta ctcggtgcga
     5161 caatcattga ttaataggta tagtgaccag tctgtggact ggtttttcgt ttttctccaa
     5221 caggaatctg tatcattaac actcttcgaa aatcaaactc agaccttgtt gacttaactt
     5281 gtaaaagaag cggtgtgatt gacggtgtga ttgaccatga taacaaattt atcaaaatgc
     5341 tttacatagg tgattacttg gtgtataata tagtctgtgt gtaatggacg cacaaaaata
     5401 cagtttatcc gctgggttta aaagcaccta tgattgacaa agataggaga ataaaatgaa
     5461 tccattgatc caaagtttga cagaaggtca acttcgtact gacatccctt cattccgtcc
     5521 tggtgacact gtgcgtgttc acgctaaggt tgtcgaaggt aaccgcgaac gtatccagat
     5581 tttcgagggt gttgttatct ctcgtaaagg tcaaggcatc tcagaaatgt acactgtacg
     5641 taaaatctct ggtggtgtag gtgttgaacg tacattccca atccacactc cacgtgttga
     5701 taagattgaa gtagtacgtt acggtaaagt acgtcgtgct aaattgtact acctacgtgc
     5761 attgcaaggt aaagcagcac gtatcaaaga aatccgtcgt taagatagtc ctacagacta
     5821 ttttggtcgg agaatgggag gcagcgaaag ctgtctcttt ttttaaaaaa aatctgttga
     5881 aagagggctc tttgtcaact gtagtgggtg gacgaaaagt taacacctag agaggacgaa
     5941 attcgttctc tcatttttga tgtttaaagc aatatatatc tttgttttaa agttcctaaa
     6001 gccgaacgct tgcctcttga tgtctttgat gagtttattg gtcgcttcca gcttag
//
LOCUS       DATUCG010000129         6019 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.131, whole genome shotgun sequence.
ACCESSION   DATUCG010000129 DATUCG010000000
VERSION     DATUCG010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6019
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.131"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            <1..578
                     /locus_tag="VCA20_002041"
     CDS             <1..578
                     /locus_tag="VCA20_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEP1835793.1"
                     /translation="VLKKAVLDKILANCKEHNILPSYAITVLHFEGLWGTSAVGKADN
                     NWGGMTWTGQGNRPSGVTVTQGSARPSNEGGHYMHYASVDDFLTDWFYLLRSSGSYKV
                     SGAKTFSEAVKGMFKVGGAVYDYAASGFDSYIVGASSRLKAIEDENGSLAKYDTATVT
                     DVGKTDKIEVAIEGIEVTINGTRYKLTKEPI"
     gene            complement(720..944)
                     /locus_tag="VCA20_002042"
     CDS             complement(720..944)
                     /locus_tag="VCA20_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP1835794.1"
                     /translation="MIQVLSKEEMMNFFQTHSRYQIAQATGISEQTLANYVKGRTDIG
                     GMSFDKVIKITEYYNSLHGKKYGYRDPKSK"
     gene            1541..2569
                     /locus_tag="VCA20_002043"
     CDS             1541..2569
                     /locus_tag="VCA20_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid lyase"
                     /protein_id="HEP1835795.1"
                     /translation="MLHFENDYNKGAHPALLEALVASNQEGLSGYGTDKYTESAIEKI
                     RLATDCPHAQVTFLAGGTQTNQIVISSMLASYEGVIAADTGHIAVHEAGAIEFTGHKV
                     LALPHTDGKLVASEVEKYISDFYADANHAHMVHPGMVYISHPTEYGTLYSKTELEDLS
                     KVCQQHNIPLFLDGARLGYGLAARETDLDLKTIAELTDVFYIGGTKLGTLMGEALVFT
                     KNNQPKNFVSIVKHHGALLAKGRVTGVQFDCLFTDDLYLKLGRHAIAMAEQLTQILEE
                     KGFQFYLKSPTNQQFVIVKNEELARLTEAGIAYGFWAKYDDSHSIIRFATSWSTSQAD
                     IDALRNRL"
     gene            complement(2588..2968)
                     /locus_tag="VCA20_002044"
                     /pseudo
     CDS             complement(2588..2968)
                     /locus_tag="VCA20_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735908.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3028..3438)
                     /locus_tag="VCA20_002045"
     CDS             complement(3028..3438)
                     /locus_tag="VCA20_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835796.1"
                     /translation="MNTNKKPAGLTNFQKYLTFIGSILGIITACITIYTFTAKPATSS
                     TSSTSPSTTISSSSATSPSQSSSDNPLTEVQDLTSSSSETTSSTQTETSSSVESTASS
                     SSAETSATQASETRTETSTDTEVTSGASETTSNQ"
     gene            3551..4693
                     /locus_tag="VCA20_002046"
     CDS             3551..4693
                     /locus_tag="VCA20_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018543459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEP1835797.1"
                     /translation="MIYFDNAATTQVYPEVLKTYTEVATKIWGNPSSLHSLGSQATRI
                     LEASRKQIAELLGKDSKEIFFTSGGTEGDNWVIKGVAFEKAHLGKHIIVSAIEHPAVK
                     ESALWLKTQGFEVDLAPVNAQGFVDVSALESLIRPDTTLVSIMAVNNEIGAIQPIQEI
                     SQLLADKPTISFHVDAVQAIGKVPTERYLTERVDFASFSGHKFHSVRGVGFVYVKAGK
                     KIAPLLTGGGQESDKRSTTENVSGIAATSKALRLTLDKAVDSQKRLVAMKQILVDELG
                     KYDDVTVFSGLEDFVPSIVTFGIKNIRGEVIVHAFEDHQIYISTTSACSSKAGKPAGT
                     LIAMGVPQKLAQTAVRISLDDDNDMGQIEQFLTIFKQIYQNTQKVR"
     gene            4695..5912
                     /gene="thiI"
                     /locus_tag="VCA20_002047"
     CDS             4695..5912
                     /gene="thiI"
                     /locus_tag="VCA20_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEP1835798.1"
                     /translation="MNYSEIMIRYGELSTKGKNKMRFVNKLRNNIKHVLSVYPEVTVY
                     FDRDRGHVYLNGADYQEVSASLKKIFGIQNFAPSYKIEKSIPALKEAVVEIMQSIYKE
                     GMTFKIAARRSDHSFELDSRELNQVLGDAVFTAIPNVQVQMKSPDITLRVEIRPDAAY
                     ISHEEIKGAGGLPVGTSGKGTLMLSGGIDSPVAGYLALKRGVEIEALHFASPPYTSPG
                     ALKKAHDLTRKLTAFGGNITFIEVPFTEIQEEIKEKAPEAYLMTLTRRFMMRITDRVR
                     EEGGAMVIINGESLGQVASQTLESMQAINAVTNTPVIRPVVTMDKLEIIDIAQEIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNVEQYEARMDVEALVERAVAGIIVTEITPKEE
                     VKDEVYSLIEDLL"
BASE COUNT         1761 a         1144 c         1353 g         1761 t
ORIGIN      
        1 ccgtcctgaa gaaagctgtg ctagataaga ttcttgctaa ctgtaaggaa cacaatatcc
       61 tacctagcta cgctattacc gttctacact ttgaggggct ttggggaact tcagccgtag
      121 gcaaggctga caacaactgg ggcggtatga cctggacagg tcaaggcaac cgtccaagcg
      181 gtgttacagt cacacaaggt tctgcccgtc catcaaacga aggtggtcac tatatgcact
      241 atgcctctgt agatgacttt cttacagatt ggttctacct gctacgttca agcggttctt
      301 acaaggtcag cggagccaag acattctctg aagccgtgaa gggaatgttt aaggttggtg
      361 gtgcagtcta tgactacgct gccagtggtt ttgatagcta tattgtcgga gcttcaagtc
      421 gtctgaaagc tattgaggat gaaaatggat cactagccaa gtatgatact gctaccgtca
      481 cagatgtcgg taagactgat aaaatagaag tagcaattga aggtattgaa gtcacaatca
      541 acggcacacg ctataaactt acaaaagagc ctatttgatt ttaacccagc gtttgctggg
      601 ctttttttgt tgcctgaatt tcaaaaaaat agcgtttttt caaaattcga tagggagata
      661 gttgccatct tgctggacat tttccaaaat ttccgttttt gcggacaaaa taaaagaccc
      721 tattttgatt tagggtctct gtaaccatat ttctttccat gcaaactatt gtagtattca
      781 gttattttga taactttatc aaaagacatg ccgccaatgt cagttcgacc tttgacatag
      841 tttgccaagg tttgctctga tatgcctgtg gcttgtgcaa tttgatagcg cgaatgtgtc
      901 tggaagaagt tcatcatttc ttccttggat aatacttgaa tcatagatac tccttatttg
      961 aaaaccaacc agagtagcaa tgcaatcagc aatgaccacg ctaggaacgc tttccagtca
     1021 aaaccatgtt tgtttacttt gtattttact ttcatagcat tttttgataa tatttaagta
     1081 cacccccgaa ggggtggata gtgattactc actatccaat tcgatgtgcc actcaatggt
     1141 taggatgata aggtttagtt tgatgaccag cttatcagtt ctaatcttga ttggcttttt
     1201 taagtaccta aacatttatt actcctttct gttagtttcc ttgtctaagg taacctcccc
     1261 ttaccttatg tatctattat actattattt ataatagtag tcaagtgttt ttataaactt
     1321 ttttgaaatt tgttaaaaaa gttcttgtcc aaaatttgtc caaaatgtcc aaaaaatgtt
     1381 agaaaaaaat aaaaatggta taataaaaaa aggctaattt atggtatttt ttatcactat
     1441 tattttctgc cagtccccca aatattccct cagggtggat taaagtgaaa agcctgcatt
     1501 aacaggcttt tttgttatat tatatcaagg aggctatgat atgttacatt ttgaaaatga
     1561 ctataacaaa ggtgcccatc ccgccttgct agaagcctta gttgcaagca atcaagaagg
     1621 tttatctgga tatggcactg ataaatatac tgaatctgct atcgaaaaaa tcagacttgc
     1681 tacggactgt cctcatgcac aagttacgtt tttagcaggg ggcacgcaga ccaatcaaat
     1741 tgtcatttcg tctatgttag cttcctatga aggagtcatt gcagcggata ctggtcatat
     1801 tgcagttcat gaagcggggg caattgaatt tacaggccat aaggtgttgg ccttaccgca
     1861 cacggatggc aagcttgtcg caagtgaagt tgagaagtat atttccgatt tctacgcaga
     1921 tgctaatcat gctcacatgg ttcatccagg aatggtttat atttcccacc cgactgaata
     1981 tggtacactt tatagcaaaa cagagctaga agatttgtcc aaagtttgcc aacagcacaa
     2041 tattccgctc tttttagacg gtgctcgctt gggttatggg cttgctgctc gtgaaacgga
     2101 tcttgatttg aagacaattg cagagctgac agatgttttc tacattggtg gtacaaaact
     2161 cggtaccttg atgggggaag ctttggtatt tacaaagaac aaccagccta agaatttcgt
     2221 atcgattgtc aaacaccatg gtgccttgtt ggcgaaagga agagtgacag gtgtccagtt
     2281 tgattgctta tttactgatg acttgtatct taaattgggg cgtcatgcta tcgcaatggc
     2341 agagcaactg acccagattt tggaagagaa aggctttcaa ttctatctca aatccccgac
     2401 caatcagcaa tttgttattg ttaagaatga agaattggct agattgacag aagctggaat
     2461 tgcctatggc ttctgggcga aatacgacga tagccacagt atcatccgct ttgcgaccag
     2521 ctggtcaact agtcaagcgg atattgatgc gttaaggaat agattataat taataaaaaa
     2581 gaaagggtta gcctttcttt ttagtttgta aataatgata gcgataaact gcataacgtt
     2641 caataaggcg gtagcctatt cctcctgcga ttgcatgtac aatcagggca atgtgcaatt
     2701 ctagtggtgc ttttatagtg ataccaaata gaataccgat tacagtccat cctagtatcc
     2761 ccaccataag tacttaaatg gagcatactg gccatcctgg tctttaactt cactggaaac
     2821 cctgtgaaca accggtcaat actcccatgg ggagcaatga acgagtatca aatggttctg
     2881 gtaaaaatga cgataaggcg aagacttgta taagtgcaaa aatcagtagc caaacgaaaa
     2941 ttggcgtttt tagaatgacg tatttcatat tgcctcctcc tgaaattagt atatcaaatc
     3001 caagaaagta aaacaaatat ttttgtgcta ttgattagaa gtcgtttcgg atgccccact
     3061 agttacctct gtatcagtac tggtttctgt ccttgtctcc gaagcttgag tagcagacgt
     3121 ttctgcagac gaggaagagg cagtagactc tactgaagag ctggtctcgg tttgggttga
     3181 ggatgttgtt tcggagcttg aacttgttaa atcttgtact tctgttaacg gattgtcgct
     3241 ggatgattgg ctggggctag tggcgctgga tgatgaaatg gtagtacttg gagatgttga
     3301 ggatgttgag gatgttgcag gttttgctgt aaaggtgtag atagtgatgc aggcagtgat
     3361 aatccctaaa atagatccga taaaggttaa atatttttga aagttagtta aaccagccgg
     3421 ttttttattt gtattcatga tgtacctcca cctttagttt aagataggaa gtttaaaact
     3481 atcttaaaga ttgccgaaag gcatgaaaac atgataaaat aggactattg caataaaagg
     3541 aatataaaaa atgatctatt ttgataatgc ggcaacgaca caggtctatc cagaagttct
     3601 caagacctat acggaagtgg cgacaaaaat ctggggaaat ccctctagcc tccatagtct
     3661 aggcagtcag gcaacccgta ttttggaagc atctcgcaag caaatcgcag aattactagg
     3721 caaggatagc aaagaaatct ttttcacctc aggtggtacg gaaggtgaca actgggtcat
     3781 caagggagtg gcatttgaaa aggcacatct gggcaagcac atcatcgtgt cagctattga
     3841 gcatccagcg gtaaaagagt ctgcactttg gctcaaaacg caaggttttg aggtggattt
     3901 ggctccggtc aatgcccaag gttttgttga tgtttcagcc ttagaaagct tgattcgtcc
     3961 agataccaca ctggtttcta tcatggccgt caataatgaa attggtgcta ttcagcccat
     4021 tcaggaaatc tctcagctct tagctgataa gccaaccatc tccttccatg tggatgcggt
     4081 gcaggctatc ggcaaggtgc cgacggaacg gtatttgaca gaacgagttg attttgctag
     4141 tttttcaggt cacaaattcc actctgtcag aggagtaggt ttcgtctatg tcaaagctgg
     4201 taagaaaatc gctccgctat tgacaggagg aggacaggaa agcgacaaac gctcaacaac
     4261 tgaaaatgtg tctgggattg cagcaacgtc taaggcgctc cgcttgacct tggacaaggc
     4321 ggtagatagc cagaagcgac tggtagccat gaagcagatc cttgtggatg aattgggcaa
     4381 atacgacgat gtgacagttt tttctggact ggaggacttt gtgccaagca tcgtaacttt
     4441 cgggattaaa aatatccgtg gcgaggtcat tgtccatgcc tttgaagacc accagattta
     4501 catttcaaca acctcagcct gctcgtccaa ggctggaaaa ccagctggaa cactgattgc
     4561 tatgggtgtt ccgcagaagt tggctcaaac tgccgtccgt atcagcttgg atgatgataa
     4621 tgatatggga caaatcgagc aattcctcac gattttcaaa caaatttatc aaaacacaca
     4681 gaaagtaagg tagaatgaac tattcagaaa ttatgattcg ctatggcgaa ttgtcaacta
     4741 aagggaaaaa caagatgcgg tttgtgaaca aactccgcaa caatatcaag catgtccttt
     4801 ctgtttaccc agaagtgacc gtttattttg accgtgaccg tggtcatgtc tatttgaatg
     4861 gggcggatta tcaggaagtg tcagcttctt tgaagaagat ttttgggatc caaaatttcg
     4921 caccatctta taagattgaa aagtctattc cagctttgaa agaagcggtt gtggagatta
     4981 tgcagtccat ttacaaggaa gggatgactt tcaagattgc ggcacgtcgt agcgaccaca
     5041 gttttgaatt ggatagccgt gagcttaacc aagtccttgg agatgcagtt tttacagcta
     5101 ttccaaatgt acaggtacag atgaaatcac cagatattac cttgcgagtg gaaatccgtc
     5161 ctgatgctgc ttatatttcc catgaagaaa ttaagggggc aggcggtctg ccagttggta
     5221 catctggcaa gggtacgctg atgctatcag gtggtattga ttcacctgtt gcgggctatt
     5281 tagcactgaa acgtggggtt gaaattgaag cccttcactt tgcaagtccg ccttatacta
     5341 gcccgggtgc acttaaaaag gcccatgatt tgactcgtaa attgactgcc tttggtggta
     5401 atatcacctt tatcgaagtg ccgtttacag aaattcagga agaaatcaag gaaaaggcac
     5461 ctgaagctta tttgatgacc ttgacacgcc gcttcatgat gcggattaca gaccgagtac
     5521 gggaagaggg tggtgccatg gttattatca atggtgaaag tctaggacaa gtggcaagtc
     5581 agaccttgga atccatgcaa gctatcaatg cggtgacaaa tacacctgtt atccgtccag
     5641 tcgttactat ggacaagttg gaaatcattg atattgctca agaaattgat acctttgata
     5701 tttccatcca gccatttgag gattgctgta ccatctttgc ccctgaccgt ccaaaaacta
     5761 accctaaaat caaaaatgtc gagcaatacg aggctcgaat ggatgtagaa gccttagtgg
     5821 aacgagctgt ggctgggatt atcgtaactg aaatcacacc gaaagaggaa gtgaaagacg
     5881 aagtgtatag tttgattgaa gacttactgt agaattcaat tcttttggca tacatttaaa
     5941 tccagctatt gctggatttt ttagttcgtt aaaatcattt gctagagtag gttaattatg
     6001 ctataatgat ttaaaagac
//
LOCUS       DATUCG010000130         6003 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.132, whole genome shotgun sequence.
ACCESSION   DATUCG010000130 DATUCG010000000
VERSION     DATUCG010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6003
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.132"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(517..1521)
                     /gene="ccpA"
                     /locus_tag="VCA20_002048"
     CDS             complement(517..1521)
                     /gene="ccpA"
                     /locus_tag="VCA20_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEP1835799.1"
                     /translation="MLNTDDTVTIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLE
                     VIDRLDYRPNAVARGLASKKTTTVGVVIPNIANAYFATLAKGIDDIADMYKYNIVLAN
                     SDENDEKEINVVNTLFSKQVDGIIFMGYHLTDKIRAEFSRSRTPIVLAGTVDLEHQLP
                     SVNIDYAAASVDAVNLLAKNNKKIAFVSGPLVDDINGKVRFAGYKQGLKDNGIEFNEG
                     LVFESKYKYEEGYALAERILNAGATAAYVAEDEIAAGLLNGVSDMGVKVPEDFEIITS
                     DDSLVTKFTRPNLTSINQPLYDIGAIAMRMLTKIMHKEELENREVVLNHGIKVRKSTK
                     "
     gene            1731..2816
                     /locus_tag="VCA20_002049"
     CDS             1731..2816
                     /locus_tag="VCA20_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEP1835800.1"
                     /translation="MSKLHHVKSYLEANKMDLAIFSDPVSIYYLTGYHSDPHERHMML
                     FVMPDHDSLLFLPALDVERAVATVDFPVAGYMDSENPWQIIKSKLPQKSFSAISAEFD
                     NLNLTRYHGLQSIFSQPFSDITPLINTMKLIKSRDEIEKMLVAGEFADKAMQVGFNNI
                     SLDVTETDIIAQIEFEMKKQGISKMSFETMVLTGDNAANPHGIPSTNKIENNALLLFD
                     LGVETLGYTSDMTRTVAVGKPDQFKKDIYNLTLEAHMAAVNMIKPGVTAGEIDYAARS
                     VIEKSGYGEYFNHRLGHGLGMSVHEFPSIMEGNDLVIEEGMCFSVEPGIYIPGKVGVR
                     IEDCGYVTKNGFEVFTKTPKELLYFEG"
     gene            complement(2855..3997)
                     /gene="tgt"
                     /locus_tag="VCA20_002050"
     CDS             complement(2855..3997)
                     /gene="tgt"
                     /locus_tag="VCA20_002050"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEP1835801.1"
                     /translation="MTDVPIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTMSPEELKAMGSGIILSNTYHLWLRPGDELVARAGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNISEEGVTFKNHLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPLDQGLFGIVQGAGFEDLRRQSAHDLVSMDFPGYSI
                     GGLAVGETHDEMNAVLDFTTPLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSRGRLVVKNAQFAEDFTPLDPECDCYTCKNYTRAYLRHLLKADETFGIR
                     LTSYHNLYFLINLMKNVRQAIMDDNLLEFRQDFMEKYGYGKNGRNF"
     gene            4601..5442
                     /locus_tag="VCA20_002051"
                     /pseudo
     CDS             4601..5442
                     /locus_tag="VCA20_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646614.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
BASE COUNT         1705 a         1286 c         1148 g         1864 t
ORIGIN      
        1 agcagaagga cagtttatgg ttattgacaa gtggtattat cgggcttgca actgcgttgt
       61 ttacacgtaa acgtaaatta taataatcac ctaactgata cagttctaaa ggatgagaac
      121 aagcccagta ctacttctct agattcgcat caacatctcg cacaacttac agagtgtgac
      181 agacttaaaa tcaaccacaa agccctcgct aagcttatac atctagcgag ggctttatat
      241 ttatttttta taatctttta gttttcgatt agtatagcca tttcgtttgc ttttagtact
      301 cgcttcaaag gttcggagaa cctttgaagg ctggagataa gtcgaagcaa gtttgcctca
      361 atagctacag gttgctagaa cagcctaaag gctgttctag gttggaaata tttcctcgct
      421 cgtcgaagta ataaaagata aactaaatga ttataatgtt tgatagagtg agggtgcgga
      481 gtaataagat atcctatcca gctactccca tatttatcac ttagttgatt tccgtacttt
      541 gattccgtgg ttaagaacta cctcacggtt ttccaactct tccttgtgca tgattttggt
      601 aagcatgcgc atggcaattg caccaatatc atatagtggc tgattgatag aggtcaggtt
      661 tggacgggta aacttagtca ctagggaatc atcacttgta atgatttcaa agtcttctgg
      721 aaccttgacg cccatatcac tgacaccatt caataaacca gcagcaatct catcttctgc
      781 aacataagct gcagttgctc cggcattcaa aatacgttct gctagagcgt agccttcctc
      841 gtatttatac ttggattcaa aaaccaatcc ttcgttaaac tcgattccgt tgtccttcaa
      901 gccttgtttg tagcctgcaa aacgaacttt accgttgatg tcatctacaa gcggccctga
      961 tacaaaggca attttcttgt tgttcttagc taatagattg actgcatcaa cactagcggc
     1021 agcgtagtca atattgacgc taggtaattg gtgctccaag tccacggtac cagctaaaac
     1081 aatcggtgta cgtgagcgtg aaaactccgc acgaatcttg tctgtcaaat gatagcccat
     1141 gaaaatgatt ccgtccacct gttttgagaa tagggtattc accacattga tttctttctc
     1201 atcattttca tcactgtttg ctaagacgat attgtatttg tacatatcgg caatatcgtc
     1261 gatacctttg gccaaggttg caaaataagc attggcaata tttggaatca caacccccac
     1321 agtggtagtc tttttgctgg ccaatccacg tgcaacagca tttggacgat aatccaaacg
     1381 gtcgatgact tctaatactt ttttacgagt attttccttt acgtttttat tcccatttac
     1441 cacgcgcgat actgtcgcca tggatacacc tgcttcgcgg gcaacgtcat aaatcgttac
     1501 cgtatcgtca gtgtttaaca tacgttatgt actccctttc aatagttctg gttctatgaa
     1561 aattacgctt tcactcaagt atagtgtatc actttttgaa accactttca agagataaac
     1621 tctatttttt tgataatact tgcaattttt ttcatttttt gaaacaatag tctatataat
     1681 aaacgaaaat ttcacttttt ttaccactac atgctagaaa ggtctccact atgtcaaaat
     1741 tacatcatgt taaatcctac ttagaagcca acaaaatgga cttggccatc ttctctgatc
     1801 cagttagcat ctattatctg actggctatc atagtgatcc tcatgaacgc cacatgatgc
     1861 tctttgttat gcctgaccac gactcactac tctttctccc tgccttggat gtagagcgtg
     1921 cggttgcgac tgttgatttt cctgtagccg gctatatgga ctcagaaaat ccatggcaga
     1981 ttatcaagag caaattgcca caaaaatctt tctcagcaat cagtgcagaa tttgataatt
     2041 tgaatctaac ccgctaccat ggtcttcagt ctatctttag ccaaccattt tcagacatta
     2101 cacctctgat taatactatg aaattgatca agtctcgtga tgaaattgaa aaaatgctgg
     2161 ttgctggtga atttgcagat aaggctatgc aggttggttt caataacatc tcactcgatg
     2221 taaccgaaac tgatattatt gctcagattg aatttgaaat gaagaaacaa ggcatctcaa
     2281 aaatgagctt tgagaccatg gtcttaacag gcgacaatgc cgcaaatcca cacggtattc
     2341 cctcaacaaa caaaatcgag aataacgctc tgctattgtt cgacctaggc gtggaaactt
     2401 tgggttatac gtctgatatg acacggactg tcgctgtcgg taaacctgat cagtttaaaa
     2461 aagatattta caacttgact ctggaggctc acatggccgc tgtcaatatg attaagcctg
     2521 gtgtgactgc tggtgagatt gactatgccg ctcgctctgt gattgaaaaa tcaggttatg
     2581 gcgagtactt caaccaccga ctcggtcacg gtttgggaat gagtgtccat gaattcccat
     2641 cgattatgga aggcaatgat ttggtcatcg aagaaggtat gtgtttctct gtcgaaccag
     2701 gtatttacat ccctggaaaa gttggtgttc gtatcgagga ttgtggttac gttaccaaaa
     2761 atggctttga agtctttaca aaaacaccaa aagaattact gtattttgaa ggataaaaaa
     2821 tagcctccta caaatctgta ggaggttgtt tattctagaa atttctgccg tttttaccgt
     2881 aaccgtattt ttccataaag tcttgacgga attccaaaag gttgtcatcc atgattgctt
     2941 gacggacatt tttcatcaaa ttaatcaaga agtagaggtt gtgataactt gtcaaacgaa
     3001 taccaaacgt ctcgtcggcc ttaagtaaat ggcggagata cgcacgtgta tagtttttac
     3061 atgtgtagca atcgcactct ggatctaacg gtgtaaagtc ctcagcgaac tgagcatttt
     3121 tcaccacaag acgtccacga ctggtcatac aggttccgtt acgcgcaatg cgtgttggta
     3181 atacacagtc aaacatatca accccacgaa ttacgccatc aatcaagcta tctggtgctc
     3241 caacccccat gagatagcgt ggtttattct ccggtaaaag aggggtcgtg aagtccagta
     3301 cagcattcat ttcatcgtgt gtttcaccaa cagccaatcc tccaatagag tatcctggaa
     3361 aatccatgct aaccaaatca tgggctgatt ggcgacgaag atcttcaaaa ccagcacctt
     3421 gtacaatacc aaataagcct tggtcaagcg gacggcggtg agccttgaga ccacgttctg
     3481 cccaacgact tgttctttcg atggattttt ttacatagtc ataaggctgg tagaactgcg
     3541 gacactcatc aaagctcatc ataatgtcag agccaagatt gttctgaata gagatagctt
     3601 tttcaggtga caggaacatc ttactcccat tgagatggtt tttgaaggtc acaccttctt
     3661 ctgagatatt gcgactatcc gccaaagaat aaacctggaa accacctgag tcagtcaaaa
     3721 tcggttgatc ccaattcata aatttgtgca aaccacctgc acgcgccacc aattcgtcac
     3781 ctggacgaag ccacaaatga taggtattcg ataggataat tcctgaaccc atcgccttca
     3841 attcttcagg cgacatggtt tttaccgttg cctgagttcc aactggcata aacatggggg
     3901 ttggaaaggt cccgtgtggg gtaatgattt cgcccaaacg tgcacctgtg tgcttttctt
     3961 tcttaatcaa gcgatacttg attggaacat ctgtcatgtt ttatttacct cccgctagga
     4021 aaaacagtcc taggatatta gttctggatt cacagttcag ctctttcata tcatccataa
     4081 aagtacttta cctggaaata cagtttctca atcttgcatg gtctaatcca tcagaatgtc
     4141 tttaatgttc tcgcttattt cagcgatatt tgtcttttct tctacttatt taatagtaaa
     4201 tagacaggcg catcacaaca aactgacaaa ttatagtcat ttagtttatc tttgattgct
     4261 tcgacgagcg aaaggttggg ctcaagacca acctcgcttc tcaggggtcg tgtcaacatc
     4321 tcagcgcagt ggttgattgg ctgaaacagt ctggggttag actgtttcag ctcaacaact
     4381 taaaactagg acttgttgac gaactttttt tgaccagtct agttctttcc cactccctat
     4441 ttccaacttc aaacactcca ctagagtatt tgaactcgct ctgccgtact tcataaaggc
     4501 aaactaaacg actataccac agcaccttcc agtctatcaa aaaaagccta gcttgtcatc
     4561 tagaactatg ttacaatgtt aagataagga ggttatgtag atgtttacaa tttctaatat
     4621 acgtgagcgg actcgccaaa cgctaaacga gacacctggt atctatcaag tggccgtcat
     4681 cccgtcacta tttctgttat cgttcagtca atttctttct ctcgcaatag ccttactgga
     4741 gtaacagctg aggaacttgc cagtcctagc tactttattt ctacctcctt gtttccacta
     4801 ttttacggat tgctattagg actactgcac ttatccatta tatgcactat atttcatctt
     4861 atcaaagggg taaaaacttc cacatcattt aaagatgtgc tgatgatttt caatcataaa
     4921 gatttctgga aaatctttcg aacctacatt ttaaaaagtt tcttgctctt cctttgggga
     4981 ctactgtttt accttggtat cggtctactt atcggtgctg gaacgatgat tacgattata
     5041 gctgcatcca gtcaatcctt agaccctgat gcacttccta tggaaatcct tgccatacta
     5101 gggctaatgg ccatgggagg tatcctactt atggttttag gaattgctct ttatattcca
     5161 caaatctatg cttactcaca ggtagaatat atcttgtttg aacaattgga acgcgaggaa
     5221 tatacagacg ccttttcaat cattaaatct agccgaaagt tgatgaaagg ttacaagttt
     5281 aaacgcttta cacttgattt atccttcatt ggctggttcc ttcttgtcat tattacattt
     5341 ggcctggctg gtttatatgt atggccgtat cactatgctg cacaaatgca cttccatgaa
     5401 gaaattttag atgatcaggc taagaaaatg agttacgtat aaaataagag cgatatttac
     5461 tcttattttt atatacactt ttatgtctat atttaagaaa aaaatatagc aataattggt
     5521 acaaacattt ggattgtgtt atactatgac tgaggagcga caaaggacta ttttagcctg
     5581 cttggtattg gagcagattt cttcctttct attttttacg ccatgtgtag taatttattc
     5641 caattctaag cgctcctcac ttagcgtcat ccagcagttc aatggttcat tgagctgctt
     5701 tttcgatgaa aaagggagtg ggaaagaact cgaccagtct aaaaagagtt cgtcaacaag
     5761 tctttatttc tagttgttga gctgaaacag tctattccca gactgtttca ctcccacccc
     5821 cgcacagctc aaactgtctg ggagacagtt tgaggttgga aatgaagcga actctgttcg
     5881 catcagtcgt agaggtcaga tttggagtgt gaaacacgaa caaatctgcc aatcaaccac
     5941 tgcgctgaga tgttgacacg aactctgagg agcgaggatg gactttctgc ccagcctcta
     6001 tta
//
LOCUS       DATUCG010000131         5953 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.133, whole genome shotgun sequence.
ACCESSION   DATUCG010000131 DATUCG010000000
VERSION     DATUCG010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5953
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.133"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            <1..169
                     /locus_tag="VCA20_002052"
     CDS             <1..169
                     /locus_tag="VCA20_002052"
                     /inference="COORDINATES: protein motif:HMM:TIGR01167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEP1835802.1"
                     /translation="GKSAIDAINTTTSAKKAHKENKVLPKTGDNVGLSSVLGIAGLAL
                     LGTAIRKKRED"
     gene            448..1584
                     /locus_tag="VCA20_002053"
     CDS             448..1584
                     /locus_tag="VCA20_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003092450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEP1835803.1"
                     /translation="MKKRPTSAYIHIPFCTQICYYCDFSKVFIKNQPVDDYLVALMEE
                     VKFYDLPALRTLYIGGGTPSALSADQLDYLLTNLEDLLDLSQVEEFTIEANPGDLTAD
                     KIAVLKKSKCNRVSLGVQTFDDRMLKKIGRSHNQAQIYETIAALKEAGFHNISIDLIY
                     ALPGQTMEQVVDNVAKALELDIPHMSLYSLILENHTVFMNRQRRGNLHLPNEDVESDM
                     FDYILQELEKNGFEHYEISNFTKPGFESRHNLMYWDNSEYYGLGAGASGYIDGMRYRN
                     RGPIQHYLKSIREKGHSRLHEEFLSQTEQMEEEMFLGLRKKTGVSIERFEEKFGISFE
                     DRYGQVVRDLKNEGLLQEEDRWLRMTKKGLFLGDTVAERFIIED"
     gene            1597..2337
                     /locus_tag="VCA20_002054"
     CDS             1597..2337
                     /locus_tag="VCA20_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEP1835804.1"
                     /translation="MGLSYQEEFTIPFDMVDVKQEIKLPDFISYCLGVSGRQSEELGR
                     SDLYVFQEFGLIWVVTDYELTIQGLPKYNETITIKTEAVAYNKFFCHRMFYIYDAAGN
                     LLLDILCYFVLIDFETRKVAPVPEDLIAPYQSEQVKKLPRAPKYQLLENPSVQEFPVR
                     YFDLDVNGHVNNGKYLEWMYEALGYDFLLCHVPQKIQLKYLKEVEATSLVSSRMVAVD
                     GISQHEIVVDGHIHAQAVIEWRERHVAG"
     gene            2324..2941
                     /locus_tag="VCA20_002055"
     CDS             2324..2941
                     /locus_tag="VCA20_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEP1835805.1"
                     /translation="MSQDKWLEWAVRLQALAQTGLAYGKDVYDIERFEEIRQIAAEML
                     VEPSGQPLEVVKDLFCNETGYQTPKLDTRAAIFQEDKILLVQENDGLWSLPGGWCDVD
                     QSVKDNVVKEVKEEAGLDVEALRVVAILDKHKNNPAKSAHRVTKVFILCRLLGGEFQP
                     NSETVASGFFSLDDLPPLYLGKNTAEQLALCLEASRSEHWETRFD"
     gene            2951..3724
                     /locus_tag="VCA20_002056"
     CDS             2951..3724
                     /locus_tag="VCA20_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEP1835806.1"
                     /translation="MTYTGYLIDLDGTIYEGKKRIPAGERFIHRLQERQIPYLFVTNN
                     TTRRPEMVQAMLAENFNIETPLETIYTASLATVDYMNDLGKEKTVYVIGEDGLKSAIF
                     EAGYVEDTENPAYVVVGLDTQLTYEKLTIATLAIQKGATFIGTNPDLNIPTERGHLPG
                     AGSLIALLKAATRVEPTFIGKPEAIIMDKALEILGTERSQTVMVGDNYLTDICAGIDN
                     GFPTLLVLTGFTKPEEVADLPLAPTHVLNSLDEWSFDEN"
     gene            3714..4343
                     /locus_tag="VCA20_002057"
     CDS             3714..4343
                     /locus_tag="VCA20_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEP1835807.1"
                     /translation="MRTKLQIIGTILFVLSAAVLGTIYLAWLVYPLEISFLGLEKVVY
                     MKAADISYNFNILMNYLTNPFASVLDMPNFSSSADGLKHFADVKHLFHLTQGIFILTL
                     PAFVLFVKNILLKSYGDLVKKVIFWTMLTPMIIGLLGVLVGFDQFFVLFHTVLFPGDS
                     TWLFDPAKDPVIYILPQEFFLHCFVLFFVLYEFFFGTILAWTGKKNRIG"
     gene            4441..4617
                     /locus_tag="VCA20_002058"
     CDS             4441..4617
                     /locus_tag="VCA20_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEP1835808.1"
                     /translation="MSKTVVRKNESLDDALRRFKRAVTKAGTLQETRKREFYEKPSVK
                     RKRKSEAARKRKKF"
     gene            4721..5734
                     /gene="galE"
                     /locus_tag="VCA20_002059"
     CDS             4721..5734
                     /gene="galE"
                     /locus_tag="VCA20_002059"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEP1835809.1"
                     /translation="MSILVTGGAGYIGSHTVVELLKLGKEVVIVDNLSNSSILVLDRI
                     ETITGKRPTFYELDVADKEALRQVFENENIEAAIHFAGYKAVGESVAKPIMYYENNIM
                     STLALVEVMTEFSVKKIVFSSSATVYGLNNPSPLVETMPTSATNPYGYTKVMLEQILR
                     DVEVADKEWSIALLRYFNPIGAHESGLIGENPAGIPNNLMPFIAQVAVGKREELSVFG
                     NDYDTVDGTGVRDYIHVIDLALGHIKALEKISTTAGVHTYNLGSGQGTSVLELVQAFE
                     KVNGVPVPYKIVDRRPGDVATCYANADKALAELNWKTEKTIEDMCRDTWNWQSKNPNG
                     YEG"
BASE COUNT         1698 a         1156 c         1414 g         1685 t
ORIGIN      
        1 tggcaaatca gcgattgacg caatcaatac aactacaagt gctaagaagg cgcataagga
       61 aaacaaggtt cttcctaaga ctggagataa tgttggttta tcaagcgtct tgggcatagc
      121 aggtttagct ttgttgggga cagcaattcg taaaaaaaga gaagattaac tatttcctta
      181 agaagttctt cagtagcatg gagttctaaa cgtgagatta taacggaact taaatactct
      241 aaagttgtgt ttagaaacct tgatttaaag gctctttgtc aactgtagtg ggttgttcca
      301 tttaataccg agagaggaca ggaattgtct tctcttgttt tatttttact attttctagt
      361 ggtagatcag aggtgtatgg aaagaagtca gtattgatta ttttgctaaa aacataaaaa
      421 gtctaacagt gtgctataat agttggtatg aaaaaacgac cgacatctgc ctatatccac
      481 attccattct gtacccagat ttgttactac tgtgactttt ccaaggtttt tattaagaac
      541 cagcctgttg atgactattt ggtagccctc atggaagagg tcaagtttta cgaccttcca
      601 gccctccgta cactttatat tggaggcgga acaccgtcag ctctctcggc agaccagcta
      661 gattatctcc tgaccaatct ggaggacctg ctggacttgt cacaggtgga agaattcact
      721 atcgaggcca atccaggtga cttgacggcg gataagatag cagttctgaa aaagtccaag
      781 tgcaatcggg tgtcgcttgg cgttcaaaca tttgatgacc gtatgttgaa aaaaattggt
      841 cgcagccaca atcaggccca gatttatgaa accattgcgg ccctcaagga agcgggcttc
      901 cacaacattt ccattgacct gatttatgcc ctgcctggtc agaccatgga gcaggtggtg
      961 gacaatgtcg ccaaggcctt ggagctagat attccccaca tgagcttgta tagtttgatt
     1021 ttggagaatc acacggtctt catgaaccgc cagcgtcgcg gcaatcttca tctgcccaac
     1081 gaagatgtgg agtccgatat gtttgactac atcctgcaag agttggagaa aaacggtttc
     1141 gaacactacg aaatctccaa cttcaccaag cccggctttg aaagtcgcca caacctcatg
     1201 tactgggaca actccgagta ttacggcttg ggagctggag catctggcta catcgacggc
     1261 atgcgctacc gcaatcgcgg ccccatccag cactacctca aatccatccg cgaaaaaggc
     1321 cattcccgcc tgcacgaaga gtttctcagc cagactgagc agatggaaga agagatgttt
     1381 ttagggctac ggaaaaaaac tggcgtttct attgagcgat tcgaagaaaa gtttggtatt
     1441 tcctttgaag accgctacgg tcaggtggtc agagacttga aaaatgaagg tcttttacaa
     1501 gaagaagacc gttggcttcg gatgaccaaa aaaggattgt ttcttggcga tacagttgcc
     1561 gagcgtttca ttatagaaga ttagaaagag gacacaatgg gactaagcta ccaagaagaa
     1621 ttcaccattc cctttgatat ggtggatgtt aaacaagaaa ttaaactgcc tgactttatt
     1681 tcctactgtc tcggtgtgtc aggtcggcag tcggaagaac tggggcgcag tgacctctat
     1741 gtgtttcagg aatttggctt gatttgggtg gtgacagatt acgaactgac cattcaaggc
     1801 ctacctaaat acaatgaaac cattactatt aaaacagaag cggttgccta caataagttt
     1861 ttctgtcatc ggatgttcta catctacgat gcagcaggca atcttttgtt ggacattctc
     1921 tgctattttg ttttgattga ttttgaaact cgcaaggtgg cgcctgtgcc agaagatttg
     1981 attgctcctt atcagtctga gcaggtcaag aaattgcctc gggctcccaa gtatcagctc
     2041 ttggaaaatc catctgttca agaatttcct gttcgctatt ttgacttgga tgtgaatggc
     2101 catgtcaata atggcaagta cctggaatgg atgtatgaag cactgggcta tgattttctg
     2161 ctctgccatg tccctcaaaa aatccaactc aagtatctta aggaagtaga ggcgactagc
     2221 ttggtgagtt cacgcatggt ggcagtagat ggcatcagcc agcatgagat tgtggtagat
     2281 gggcatatcc atgctcaagc tgtcatagaa tggagggagc gtcatgtcgc aggataagtg
     2341 gttggaatgg gccgtacgtt tgcaagcctt ggctcagaca ggtttagcct atggaaaaga
     2401 tgtttatgac atagagcgtt tcgaagaaat caggcagatt gcagcagaga tgttagtaga
     2461 accgtctggg cagcccttgg aagtagtgaa agacttgttt tgcaatgaaa caggctatca
     2521 gacacctaag ttggatactc gcgcagctat tttccaagag gataagatcc tactggttca
     2581 ggaaaacgat gggctctggt ccttaccagg tggttggtgc gatgtggacc agtccgtcaa
     2641 ggacaatgtg gtgaaagagg tcaaggaaga agcggggctg gatgtggagg ctctgcgagt
     2701 cgttgcgatt ttggacaagc acaagaacaa tcctgccaag tcagcccacc gcgtgaccaa
     2761 ggtcttcatt ctatgtcgtc tccttggcgg ggagtttcag cccaattcgg aaacagttgc
     2821 cagtggcttt tttagtctag atgacttacc accgctctac cttgggaaaa atacagctga
     2881 gcagttagca ctttgcttgg aagctagtcg gtctgagcat tgggaaacac gatttgatta
     2941 aggaggaaaa atgacgtata caggttattt gattgatttg gatggtacga tttatgaagg
     3001 gaaaaagcgg attcctgctg gtgaacgttt tattcatcgc ttgcaagaac gtcagattcc
     3061 ctatttattc gtgaccaaca ataccacccg ccgtcctgag atggtgcagg ctatgttggc
     3121 tgaaaacttt aacattgaga caccgcttga aaccatctat acagccagtc tggcgacagt
     3181 agattatatg aatgacttgg gcaaggaaaa gacagtctat gtcattggag aagacgggct
     3241 caagtctgcc atttttgagg caggttatgt ggaagacacg gaaaatccag cctatgttgt
     3301 tgtcggtttg gatactcagt tgacctatga aaaactgacc attgccacct tggctattca
     3361 aaagggagcg acctttatcg gtaccaatcc tgatttgaac attccgactg aaagaggtca
     3421 tttgccagga gcaggctcac ttattgcgct tttgaaagct gctacccgag tagagccgac
     3481 ctttatcggt aagcctgaag ccattatcat ggacaaggct ctggagattt taggcacaga
     3541 acgcagccag acagttatgg taggagataa ctacctgaca gatatttgtg cagggattga
     3601 caatggattt ccaactcttt tagtcctgac aggctttacc aagccagaag aagtagcaga
     3661 cttacccctg gctccaaccc atgtcctcaa cagcctagat gaatggagtt ttgatgagaa
     3721 ctaagttaca gattatcgga acgattttat ttgtcttgtc ggcagcagtt ctaggaacta
     3781 tctacctagc ttggctagtt tatccgcttg agatttcttt tttggggttg gaaaaagtgg
     3841 tctacatgaa ggcggcagat atttcctaca atttcaatat cctgatgaac tatctaacaa
     3901 atccttttgc aagtgtttta gacatgccaa atttttcatc ctcagctgat ggtctgaaac
     3961 attttgcgga tgtgaaacat ttattccatc taactcaggg gatttttatc ctcacactcc
     4021 ctgcctttgt gctttttgta aaaaacattc tgctaaaaag ttatggagac ttggttaaaa
     4081 aggtcatttt ctggaccatg cttactccaa tgataatcgg tttattaggt gttcttgttg
     4141 gctttgatca gttttttgta ttgtttcata cggtgctatt tccaggagat tctacatggc
     4201 tatttgaccc tgcaaaggat cctgtcatat atattttgcc acaagagttt ttcttgcatt
     4261 gctttgtatt attttttgtc ctttacgaat tcttctttgg gacaatactg gcgtggacag
     4321 gaaaaaagaa taggatagga taagagcgta aaattcatat tttagaatga aaaaatattg
     4381 acaaatgagt tgaatcatag taaaatgata aagactttga aattgagggg agtgaaaaaa
     4441 atgtcaaaaa cagtagtacg caagaacgaa tcacttgatg atgctcttcg tcgtttcaaa
     4501 cgtgcggtta ctaaagctgg tactcttcaa gaaacacgca aacgcgaatt ctacgaaaaa
     4561 ccatctgtaa aacgtaaacg taaatcagaa gcagctcgca agcgtaaaaa attctaattg
     4621 aatttgaaaa cggccgaaag gctgtttttt gtttactttt tacaacaggt aacatataat
     4681 agtgatatag aattttgtcc aggtgaaaag gagtaaatag atgagtatct tagttacagg
     4741 tggtgcaggt tatattggta gtcatacggt tgtcgaattg ttgaagttgg ggaaagaagt
     4801 ggttatcgtg gataatcttt caaactcaag tattttggtt ttagatcgca ttgaaaccat
     4861 cactggcaag cgcccaactt tctatgagtt ggatgtggca gataaggaag ccttgcgcca
     4921 agtttttgaa aatgaaaata ttgaagcagc cattcacttt gctggctaca aggcagtagg
     4981 agaatcagta gctaagccaa ttatgtacta tgaaaacaat atcatgtcaa ccctagcttt
     5041 ggtggaagtc atgacagaat ttagtgttaa gaaaattgtc ttctcctcaa gtgcgactgt
     5101 ttatggcctc aataatccat cgcctttggt tgaaactatg ccaactagcg caacgaatcc
     5161 gtacggctat accaaagtta tgttggaaca aatcttgcgt gatgtggaag tagcagataa
     5221 ggagtggagt attgcattgc tccgttattt caatccaatc ggtgcccatg aatctggctt
     5281 gattggggaa aatccagctg gtattcctaa taatttgatg ccgtttattg cacaggtagc
     5341 cgttggtaaa cgtgaggaac tcagcgtttt tggtaacgat tatgatacag tggatggaac
     5401 aggtgttcgt gactatatcc acgtcattga cttggctctt ggtcacatca aggcgttgga
     5461 aaaaatctca acgacagcag gtgtccatac ctataacctt ggttctggtc aaggaaccag
     5521 tgttttagaa ctggttcagg cttttgaaaa agtgaatggt gtaccggtac cttataaaat
     5581 cgttgaccgt cgtccgggtg atgtagcgac ctgctatgcg aatgcagaca aggctttagc
     5641 agaactcaac tggaaaacag agaaaacgat tgaagatatg tgccgcgaca catggaactg
     5701 gcaatcgaaa aatccaaatg gatacgaagg ataagaaaag atgtgagtgg aggctcacat
     5761 cttatttttg taattatgga agagaggctg ggcagaaagt ccatcctcgc ttctcagagt
     5821 tcgtgtcaac atctcagcgc agtggttgat tggcagattt gttcgtgttt catactccaa
     5881 atctgaccta atcaactgtg cgggggtggg aagacgaact ctttttagac tggtcgagtt
     5941 ctttcccact ccc
//
LOCUS       DATUCG010000132         5827 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.134, whole genome shotgun sequence.
ACCESSION   DATUCG010000132 DATUCG010000000
VERSION     DATUCG010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5827
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.134"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(241..534)
                     /locus_tag="VCA20_002060"
                     /pseudo
     CDS             complement(241..534)
                     /locus_tag="VCA20_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637363.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
     gene            complement(531..1472)
                     /locus_tag="VCA20_002061"
     CDS             complement(531..1472)
                     /locus_tag="VCA20_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEP1835810.1"
                     /translation="MRQVILDKVSEQIEQGVYPGASLAFFSEGQWQEYYLGTQDGHMP
                     VEAGLTYDLASVSKVVGVGTLATFLVNSGALELDRTLQSYYPAFADCQVTIRQLLTHT
                     SGIDPFIPNRDSLDADQLKEAILKIAVTDKKAFLYTDINFLLLGFLLEELGGASLDQL
                     IRRDVLEPFGMFQTSFGPVSQAVPTVRGVKAGVVHDPKARVLGVHAGSAGLFSTVKDL
                     EIFLEHYLTADFAANLTQDYGFDDKRKRSLAWDKKGAWLSHTGYTGTFIMYNRPLQKA
                     AIFLSNRTFDKDERAQWKLDRNQVMPLIRQVLEEEGQ"
     gene            complement(1469..2203)
                     /locus_tag="VCA20_002062"
     CDS             complement(1469..2203)
                     /locus_tag="VCA20_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HEP1835811.1"
                     /translation="MLRKNEAIVPVLRINNRAINQGFLEKNLGMRTKLEDGPFADFGD
                     RTSPETKLVLTESPGNRTRAVEGLKKLNKIVIKVDNASEIEALLAQGSQYSKLYQGKN
                     GYGFEALSPEGDTFLLHAEASVDDLKEILPPVPFKVQDDFSGLTAFTVESVWINTPQA
                     SISQDYYEAILPQQAFLRFVGAEGKDLLTPAEQVWDLEGLRFPVEQDFDWAHLESQLE
                     GPFFKDKKASFIQTVDPSGIELWFEK"
     gene            2385..4652
                     /locus_tag="VCA20_002063"
     CDS             2385..4652
                     /locus_tag="VCA20_002063"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HEP1835812.1"
                     /translation="MRFNQFSFIKKETSIYLQELDALGFQLIPNASSKTNLETFVRKC
                     HFLTANTDFALSNMIADWETDLLTFFQSDRELTDLIFYQVTFQLLGFVPGVDYTDVMV
                     FVEKSNFPVIYGDVIDNLYQLLATRTKSGNTLIDQLVSDDLIPEDNRYHFFNGKSLAT
                     FSTKNLIREVVYVETPVNTAGPGQTDIVKLSILRPQFDGKIPAVITNSPYHQGVNEVA
                     SDKALHKMEGELAEKPAGTIEVNQTNITKLDLDQRELPISAATEKLGHMTSYSLNDYF
                     LARGFASLHVSGVGTLGSTGYMTSGDYQQVEGYKAVIDWLNGRTKAYTDHTRSIEVKA
                     DWANGKVATTGLSYLGTMSNALATTGVDGLEVIIAEAGISSWYDYYRENGLVTSPGGY
                     PGEDLDSLTALTYSKSLQAGDFLRNKAAYEKGLAAERAALDRTSGDYNQYWHDRNYLL
                     QADKVKCEAVFTHGSQDWNVKPIHVWNMFHALPSHIKKHLFFHNGAHVYMNNWQSIDF
                     RESMNALLSQKLLGYENNYQLPTVIWQDNSGEQTWTTLDTFGGENETVLPLGTGSQTV
                     ANQYTQEDFERYGKSYSAFHQDLYAGKANQISIELPVTEDLLLNGQVTLKLRVASSVA
                     KGLLSAQLLDKGNKKRLAPIPAPKARLSLDNGRYHAQENLLELPYVDMPQRLITKGFM
                     NLQNRTDLMTVEEVVPDQWMDMSWKLQPTIYQLKKGDVLELILYTTDFECTVRDNSQW
                     QIHLDLSQSQLILPH"
     gene            complement(5037..5753)
                     /locus_tag="VCA20_002064"
                     /pseudo
     CDS             complement(5037..5753)
                     /locus_tag="VCA20_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922585.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
BASE COUNT         1592 a         1404 c         1207 g         1624 t
ORIGIN      
        1 gtcaagtgaa ttagaaacgg gacatatcta agtttgtcaa tttgagaact ctaaagttat
       61 tttaggagtg ggttattaac actataactt aaggatgtca ttttcctaat tcaagccgct
      121 atatcaagta agtgagcttt aatctgatct aatatgactt ttcccctttt atttttagtg
      181 taacataaaa ataacccacc acaaaatgtg ataggttagt taacttaatg acattgcgtt
      241 tctcgccaat tcttcttgcc gccaagctgg aagctgatca atttctgctt gataatcttg
      301 ggcgtctagt agagcaaatt gctccacaaa ggggtatctt tctgtgaaaa aagtttgaaa
      361 ttcagcaaca atgtcccctc tattcttggt tgaatcagag aagagattac cgctattaat
      421 gtaggtttca accttgtcat agcccaaatc cgccaccgct tgacgcaatt cagccatgac
      481 aattttgtta cgaccaccga cattgatccc tctcagcaga agaacatatc tcattgcccc
      541 tcttcctcca agacctgacg aatcaagggc ataacctggt ttctatccaa cttccactgg
      601 gcccgctcat ccttgtcaaa agtacgattc gagagaaaaa tcgcagcttt ctgcaaggga
      661 cggttataca tgataaaggt ccccgtatag cctgtgtgcg acaaccaggc ccccttctta
      721 tcccaagcca gagaccgctt gcgcttatca tcaaaaccgt aatcctgcgt taaattcgcc
      781 gcaaagtcag ccgtaagata gtgttccaaa aagatttcca gatccttgac cgtcgaaaac
      841 aggcccgcac tacccgcgtg aacacccaag acacgcgcct tgggatcatg caccacacct
      901 gctttgacgc cacgcaccgt cggtaccgcc tgactgactg gtccaaagga ggtttggaac
      961 ataccaaagg gctctagaac atctcgtctg attagctggt ctagacttgc tccacccagt
     1021 tcctccaaca aaaagcccag caagagaaaa ttaatgtctg tgtagaggaa agctttctta
     1081 tccgttacag caatcttcaa aatcgcttcc ttgagctggt cggcatccaa actatcccga
     1141 ttgggaatga aggggtcaat accactagta tgggtcaata attgccgaat ggtgacctga
     1201 caatccgcaa aagctggata ataagactgc aaggtcctat ctagctccaa agctccgcta
     1261 ttaaccaaaa aagtcgcaag agtccctact ccaaccacct tcgaaacaga cgccaaatca
     1321 taggtcaaac ccgcctcaac tggcatatgc ccatcctgag tccccagata gtactcctgc
     1381 cactgaccct cagaaaaaaa agccagactg gcaccaggat atactccttg ctcaatctgc
     1441 tctgaaacct tgtctaaaat tacttgtctc atttttcgaa ccacaattca ataccacttg
     1501 gatcaaccgt ttgaataaag cttgcttttt tatctttaaa gaatggtcct tccaactggc
     1561 tttccaaatg cgcccaatca aagtcctgct caacgggaaa acgcaagcct tctaaatccc
     1621 aaacttgttc tgcaggagtt aataaatcct taccttctgc tccaacaaaa cgcaaaaagg
     1681 cttgttgagg taggatagcc tcataatagt cttggctaat actagcttgt ggggtattaa
     1741 tccagacaga ctctactgta aaagcggtca gaccagaaaa gtcatcctgc accttaaatg
     1801 gaacaggtgg aaggatttct tttaggtcat caacagatgc ttctgcatgt aaaagaaaag
     1861 tgtctccttc tggcgaaaga gcttcaaaac catagccatt ttttccttgg tagagcttac
     1921 tatactgtga cccttgcgcc agtaaagctt caatttcaga ggcattgtct acttttatca
     1981 caattttatt caatttttta agcccttcga cagcacgggt gcgattacca ggagattctg
     2041 tcaaaacgag cttcgtttct gggcttgttc tatctccaaa atcagcaaaa ggaccatctt
     2101 ctagcttggt tctcattcct agattttttt ctaaaaatcc ttgattaata gccctattat
     2161 taatacgtaa aacagggact atagcttcat tttttctcaa cacgatacat ttcactcctc
     2221 agtcattcta caatattgta taaaaaattt cgtcatttta caactaatta tatcgtcgtt
     2281 ttatacatta atatctctat atatactgat aatttttaga caaacattga cttatccgat
     2341 ataatagcta ttatagacta ttatcctaaa aaagaggaga atttatgcgc tttaatcaat
     2401 tttctttcat aaaaaaagag acctctatct acttgcaaga acttgatgct ctaggatttc
     2461 aactgattcc taatgcaagt agtaagacaa atttagaaac ctttgtcaga aagtgccatt
     2521 tcctgacagc aaacaccgac tttgccctgt ccaacatgat tgcggattgg gagacagatc
     2581 ttctgacctt cttccagtct gaccgtgagc tgactgacct gattttctat caagtgacct
     2641 ttcagttgct gggcttcgtt cctggtgtag attatacaga tgtcatggtt ttcgtggaga
     2701 aaagcaattt ccctgtcatc tatggagatg tgattgacaa cctctatcaa ctgctggcta
     2761 ctcgtaccaa gtctggaaac accttgattg accagctggt cagcgatgac ctgattccag
     2821 aggacaatcg ctaccacttc ttcaacggta aatcgctagc gaccttttca accaaaaacc
     2881 tcattcgaga ggtggtctat gtcgaaacgc ctgtcaatac ggctgggccc gggcagacag
     2941 atattgttaa gctgtccatt ctccgtccgc agtttgacgg gaaaatccct gcggtgatta
     3001 ccaatagtcc ttatcaccaa ggtgtcaatg aagttgccag cgacaaggcc ctgcacaaga
     3061 tggaagggga gttagcagaa aaaccagctg gcaccattga ggtcaatcag accaacatca
     3121 ccaaactgga cttagaccaa cgcgagctac ctatcagcgc tgctaccgaa aaactggggc
     3181 acatgacttc atactccctc aatgactact tcctcgctcg tggcttcgcc agcctccatg
     3241 tgtctggtgt cggtacgctg ggatcgacgg gttacatgac atctggcgac taccagcagg
     3301 tggagggcta taaagcggtg attgactggc tgaacggtcg caccaaggcc tacacagacc
     3361 acactcgctc gattgaagtc aaggccgatt gggccaatgg taaggtagcg acgacgggac
     3421 tgtcttatct cggtaccatg tccaatgcct tggcaacaac tggcgtggac ggattggaag
     3481 tcatcatcgc agaagcaggg atttcctctt ggtatgacta ctaccgtgaa aacgggctgg
     3541 tgaccagccc tggtggctat ccaggcgaag atttggacag cttgaccgcc ctgacctact
     3601 ccaagagttt gcaagcaggt gacttcctcc gcaataaagc agcctacgaa aaaggactgg
     3661 cggcagaacg tgctgccctg gaccgcacca gtggcgacta caaccagtat tggcatgacc
     3721 gcaactacct gctccaggct gataaggtca agtgtgaggc ggtctttacc cacggctcac
     3781 aggactggaa tgtcaaacca atccacgtct ggaatatgtt ccacgccctg ccaagtcaca
     3841 tcaaaaagca cctcttcttc cacaacggtg cccacgttta tatgaacaac tggcagtcca
     3901 tcgacttccg tgagtctatg aacgccctgc tcagccaaaa actgctgggc tacgagaata
     3961 actatcaact accaactgtc atctggcagg acaacagcgg tgaacaaact tggacgacct
     4021 tggacacctt tggcggtgaa aacgagactg tcttgccact aggtactgga agccagaccg
     4081 ttgccaatca gtatacccaa gaagattttg aacgctacgg caaatcctac tctgccttcc
     4141 accaagacct ctatgcaggc aaagccaatc aaatcagcat tgaactgcct gtaacagagg
     4201 acctcttgct taacggacag gtcacgctga aactccgtgt cgcttctagt gtagctaaag
     4261 gtctcttatc cgctcagcta ttggacaagg gaaataaaaa acgcctagcc ccaatccctg
     4321 cgcctaaagc aagattgagt ctagacaacg gtcgttacca tgcccaagaa aatctgctgg
     4381 agttgcctta tgtggatatg ccacaacgct tgataaccaa gggctttatg aacctgcaga
     4441 accgcaccga cctaatgact gttgaggaag tggtgccaga ccagtggatg gatatgagct
     4501 ggaagctgca gccgaccatt tatcagctga aaaaaggtga tgtcttagag ctgattctct
     4561 atacaaccga ctttgagtgt actgttcggg ataattctca atggcaaatc caccttgatt
     4621 taagccaatc acaattaatt ttaccgcact aatactcaat gaaaatcaaa atcagacaga
     4681 ctagcttcaa aggtttggga aacctttgaa ggttggagat agagtgaacg aagttcgttt
     4741 ctccaagagc agatataccc ctgctacttt cttattttat ggtagcaggc tgtcaagctc
     4801 cactggagct tgacactcat caaatcaagt caacaacgtc tgattttgag tttcgaagag
     4861 tatagtcaaa tgaattaact ttcagacaag gaactgagac gcggacagta cttgagtaca
     4921 gcaagtcgaa agtgacgatg tatcaaagtt aattcaaaag actatataat attgattttc
     4981 agttctaaaa agaggctggg cgtttccgcc ctgcctcttt attatttcat aatggtttag
     5041 atttgatctt tagatttttt gtttatagct gtagcgacta ctgccaagct aattccagct
     5101 aaaccaagac ctactaaggc tacgttattg cgttgttcac ctgttgcagg tagggttgct
     5161 ttgtttgcag aaggtgcgcc cttatgactc agtgtcggat ttgattgtac tgaaggagtt
     5221 gattttactt cttctttctt catttccaag ctacctttta caagaatacg attttgacct
     5281 tcaacaacag tctctgacaa taggttggtt gcgacaactt taccatcaac tatgacatct
     5341 tgataaacag atactttttt acctgctttt ccttcttgaa gaaggtatga tttgccaagc
     5401 gctaggcttg catcatatct gatttcttct ttgataggga gagcttcttc taccttgcgt
     5461 tcttttgttg tgaagtctag ctttggtttt tccgctgttt ttggtgcgac ttctggaact
     5521 gctgtcttag gtgcagagtc ttccgctttt ggttgctcag cctttggcat ttcctccttc
     5581 ggcatgtctt ctgctttcgg ttgctccatt tctgtttgct tagcctctgg catctgtttg
     5641 tcagactcta ctttatcagt tttcggtttg tccattggag gttctggcat tggagttggg
     5701 gctggaactt cttcctctgc tttcggcgct ggagcatctg gcattggagc tggtgctgcg
     5761 tccggagttg ggactggggt ttcttcctct tttggagccg gtgcctctgg cattggagtc
     5821 ggaattt
//
LOCUS       DATUCG010000133         5772 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.135, whole genome shotgun sequence.
ACCESSION   DATUCG010000133 DATUCG010000000
VERSION     DATUCG010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5772
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.135"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            164..619
                     /locus_tag="VCA20_002065"
     CDS             164..619
                     /locus_tag="VCA20_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003090333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEP1835813.1"
                     /translation="MEFTKDNILVNVNVKDKIELFHYIAKYAKEKSIVSNESELVEAF
                     LEREKEVSTGLQETFAIPHAKADFIKKPTVLFLKLDHSIEWETFDGQPVSNVFALLVP
                     SENKGTLHLEMISKIATSLLEEEFINSVKNSSDNDELEKQIKKAMEGAY"
     gene            621..935
                     /locus_tag="VCA20_002066"
     CDS             621..935
                     /locus_tag="VCA20_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HEP1835814.1"
                     /translation="MKIVGITSCPAGLAHTPMAAKALEKAGPNLGHEVRIEQQGSMGQ
                     VNKLTPQEIAEADFVLLATVQKIVQEERFEGKKKIRVNISTCIKAPEAVLTKCVEAVS
                     KK"
     gene            979..2073
                     /locus_tag="VCA20_002067"
     CDS             979..2073
                     /locus_tag="VCA20_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HEP1835815.1"
                     /translation="MKKFLNTSKGHLMTGISYALPMIIGASLVVAIPKMIALAMGITS
                     LDPYAETAGFTHYLYQIEQVGWSGIGLINTVLAGFIAYSIADKAALGAGLIGGAIASK
                     TNAGFLGAMIAGFVAGYVVVWCKNNVKVPEKYNSILPLIVFPLFATMSVALVMGVLLA
                     GPLGAINTGLVAWIKDMIENDVNKVLLALIMGAMIGSDLGGPINKASWMAGNVLLAEG
                     IYLPAIIVNVAICAVPMGYALASLFHKNKMSDELLDASRNNYVMGFIGITEGAIPFTM
                     VNPLKLVPINMIGGAIASALGIALGMYDKMPPVGGVYGFFTVGNGWAYLIGLFAGAAF
                     IGFVAPMLVNFKESASNEDVSVDDVEISFE"
     gene            2135..4804
                     /gene="mngB"
                     /locus_tag="VCA20_002068"
     CDS             2135..4804
                     /gene="mngB"
                     /locus_tag="VCA20_002068"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012961889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HEP1835816.1"
                     /translation="MKKTVHIVPHSHWDREWYFTTSRSKIYLMHNLKKVITLLKEENG
                     YDTYTLDGQASLLDDYLAWCPEDREVIQELVQEGKLIIGPWYTQTDQMVISGESIVRN
                     MLYGMNISKEFGDYMNVGYVPDSFGQSAAMPQIYKEFGINDTLFWRGVSDDDVKQSEF
                     KWRGEDGTTVNAYQIQAGYYIGGDIPEDDERLSEYLHKEPYSRIWSRSSTDQVVFPNG
                     FDQAPARENLVDLVERMKNFYEGEFEFKISTYADYIQSIKDRHPELEEIAGELLNGKL
                     MRIHKSIFSSRSDLKKLNTQVQNYLVNVLEPLLSFSQSLGFEYPVEVVKEIWKLMFEN
                     AAHDSIGSCVSDTTNEDVYLRYKKARDLAENLVELKMREIVMRIDTDQDITATVFNLS
                     GREKSGVFETIFYVPQHQFAIKDVEGNSYEYTIISSEDQTDYILGQGNVLDSTKSTYK
                     PSKVFKVQLAIKFENLPSFGYKNFYLDLGGDTHQEQKSSTINTIENQYYRITVNAENT
                     IDILDKVSGKLYRNQAVLQNNGDDGDSFNYSPPREDLVINSNEFIPEISTNISSLIST
                     MSLQYDFKIPKNLNERAEGLRTSDLPIKLEITLKDSSPVIEFNFEVDNRFVDSHRMCV
                     LFNSEIASKFSKADHQFGAIKRPVITDDMALWKKEPEKWNEVPVAIETCQSYVTLDNE
                     ERGIAVIPKGVREYEIVGDDFDTIRLTLFRTYGNMGRENLLYRPGRASGETVIETPDA
                     QCHKVMSYQFSAIYYKGDMNSYQLANVVSEYLKDAQIYQYADYLNTRLRFTQFEVEKS
                     LNTISDMFNLNNDGILSVVKKAEERPGYILRLYNGQYLQSISPEITFSKHPSVVEFVN
                     LKEETKESLDIANEKILLGDIGHNKFITLYVEF"
     gene            4820..5706
                     /gene="manA"
                     /locus_tag="VCA20_002069"
                     /pseudo
     CDS             4820..5706
                     /gene="manA"
                     /locus_tag="VCA20_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062439.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
BASE COUNT         1928 a          820 c         1185 g         1839 t
ORIGIN      
        1 accataaaag tatttttgaa aattttgata tatttcaaaa tataagttga aaaaaataaa
       61 aaatattttt tactctttgt ggaaataaat tggaaaaagg tattagtgac tttgtttaat
      121 tttgatgtta agatgtatct gtaaaacgaa gggaggacaa gtaatggaat ttacaaaaga
      181 taatatttta gtaaatgtta atgtaaaaga taagattgaa ctatttcatt atattgccaa
      241 atatgccaag gagaagagta tcgttagtaa tgaatctgag ttagtagaag catttctgga
      301 gagggaaaaa gaagtttcaa caggattaca agagacattt gctattcctc atgctaaggc
      361 agattttata aaaaaaccta ctgtactttt tttaaaatta gatcattcca ttgaatggga
      421 aacatttgat ggtcagccag tatcaaatgt ttttgcttta ctagtaccga gtgaaaacaa
      481 aggaacactt catttggaga tgattagtaa aattgctaca tcattgctgg aagaagaatt
      541 tatcaattca gtaaaaaatt catctgataa tgatgagtta gaaaaacaaa tcaagaaagc
      601 tatggaagga gcttactaaa atgaaaatcg taggaattac atcgtgccca gcaggtttag
      661 cccatacacc tatggcagct aaagcattgg aaaaagctgg accaaattta ggtcatgaag
      721 tacgtattga gcaacaaggt tctatgggac aagttaataa gttgactcca caagagattg
      781 ctgaagctga ttttgtatta ttagcgactg ttcagaaaat tgtccaagaa gaacgatttg
      841 aaggtaagaa aaaaattcgt gttaatattt caacttgtat taaagcacct gaagctgtat
      901 tgactaaatg tgttgaagcg gtgtctaaaa agtaaataaa gttaaataaa taaacaaaaa
      961 aataaaggag taattgagat gaaaaaattt cttaatacat cgaaaggtca tttgatgacc
     1021 ggtattagtt atgctcttcc aatgattatt ggtgcatcac tagttgttgc aattcctaag
     1081 atgattgctt tagcaatggg gattacaagt ttagatccat atgctgaaac tgctggattc
     1141 actcattatt tatatcaaat tgaacaagtt ggttggtcag gtattgggct gataaataca
     1201 gttcttgcag gttttattgc ttattcgatt gctgataaag cagctcttgg tgctggttta
     1261 attggaggtg caatagcatc aaaaacaaac gcaggtttcc ttggtgctat gattgcgggt
     1321 ttcgtagcag gttacgttgt tgtatggtgt aaaaataatg taaaagtacc agaaaaatac
     1381 aatagtattc tgccattgat agttttcccg ctttttgcaa caatgtctgt tgcgttagta
     1441 atgggtgttt tgcttgcagg tccgcttggt gcgattaata caggtcttgt tgcgtggatt
     1501 aaagacatga ttgaaaatga tgttaataaa gttttacttg cacttattat gggagctatg
     1561 attggttctg atcttggcgg tcctatcaat aaagcatcat ggatggctgg taatgttttg
     1621 ttagctgaag gtatatatct tccagcaatt attgtcaatg tcgctatttg tgctgtacca
     1681 atggggtatg cattagcatc actcttccac aaaaataaaa tgagtgacga attgttggac
     1741 gcaagccgta ataattatgt tatgggattt atcggaatta ccgaaggagc aattcctttt
     1801 actatggtaa atccacttaa actcgttcca attaatatga ttggaggagc tattgcatct
     1861 gcacttggta ttgcattagg tatgtatgat aaaatgcctc cagtaggtgg tgtgtatggt
     1921 ttcttcacag ttggaaatgg ctgggcttat cttatcggtt tgtttgctgg agcagcattt
     1981 attggatttg tagctcctat gcttgtaaac tttaaggaaa gtgcttcaaa tgaagacgtt
     2041 agtgttgatg atgtcgagat tagtttcgag taggtgaagt attattagca catatcaaat
     2101 tggatatgtg cttttttaac agaaaggtta aattatgaaa aaaacagtcc atatagtacc
     2161 tcattctcac tgggatagag agtggtactt tacaacaagt cgttcaaaaa tttatttgat
     2221 gcataactta aaaaaagtta ttaccttact gaaagaagaa aacggctatg atacatatac
     2281 attagatggg caagcatcat tattggatga ttacctagca tggtgtccag aagacaggga
     2341 agttatccaa gagttagtcc aagaaggtaa attgattatt ggaccgtggt atacacaaac
     2401 tgaccaaatg gttatttctg gtgagagtat tgttagaaat atgctttatg gtatgaatat
     2461 ctcaaaagag tttggtgatt acatgaatgt aggctatgta ccagattctt ttggtcagtc
     2521 agcagcgatg ccacaaattt ataaagagtt tgggattaac gatactctat tttggcgtgg
     2581 cgttagtgat gatgacgtaa aacaatcaga atttaaatgg cgtggagaag atggtactac
     2641 tgtaaatgcc tatcaaatcc aagctggtta ctatatcggt ggcgatattc cagaggatga
     2701 tgagcggtta agtgaatatc ttcataagga accctatagt agaatttggt cgagaagtag
     2761 cactgaccaa gttgtctttc ctaacggatt tgatcaagca ccagctagag aaaacttagt
     2821 tgatttagtt gagcgtatga agaattttta cgaaggagaa tttgagttca aaatttcaac
     2881 ttatgcagac tatatccaat ccattaaaga tcgacatcca gagttggaag agattgctgg
     2941 agaacttcta aatggtaagt tgatgcgtat tcataaatca attttttcat cacgttcaga
     3001 cttgaagaag ttgaatacgc aagtacaaaa ttaccttgtt aatgtacttg aacctttatt
     3061 atcattctct caatccttag ggtttgaata cccagtcgag gtggtgaaag aaatttggaa
     3121 gttgatgttt gaaaatgcag ctcatgattc aattggttca tgtgtttcag atactacaaa
     3181 tgaagatgta tatttgagat ataagaaagc tagggacttg gctgaaaact tagtagaact
     3241 gaaaatgcgt gagattgtca tgaggattga tacagaccaa gatatcacag ctactgtatt
     3301 caatttatca ggtagagaaa aatctggagt atttgagaca atattctatg ttccccaaca
     3361 tcaatttgct attaaagatg ttgaaggaaa tagttatgag tacacgatta tttcttcaga
     3421 agatcaaaca gactatattt taggacaagg aaatgtttta gattcaacta aatctaccta
     3481 taagccttcg aaagtcttta aagttcagct tgccattaag tttgagaact taccatcttt
     3541 tggttataag aatttttatc ttgatttagg tggagataca caccaagaac aaaaatcgtc
     3601 tacaataaat accattgaaa atcagtatta tcgtattaca gtgaatgctg aaaatacaat
     3661 tgatatttta gataaggtat caggcaaact ttatcgtaat caagctgttt tacaaaataa
     3721 cggtgatgat ggcgattcat ttaattattc tccccctaga gaagatttgg ttattaactc
     3781 caacgagttt atcccagaaa tatcgacaaa catttctagt ttaatcagta caatgagttt
     3841 gcaatatgat tttaaaatac caaaaaatct taatgaacgt gcagaagggc ttagaacaag
     3901 tgatttgcca attaaattag agattacatt gaaagactcg tcgccagtaa tagaattcaa
     3961 ttttgaagta gataatcgtt ttgtggatag ccatcgcatg tgtgtattgt ttaacagtga
     4021 aattgcatct aaattctcta aagcagatca tcaatttggt gcaattaaac gtccagtaat
     4081 tacagatgac atggcactct ggaaaaaaga accagagaaa tggaatgagg ttcctgttgc
     4141 aattgaaacg tgccagtcat atgttaccct agataatgaa gaacgtggta ttgctgttat
     4201 ccctaaaggt gtccgtgaat atgaaatcgt tggcgatgat tttgatacaa ttcgcttaac
     4261 tttgtttaga acatatggca atatgggaag agaaaacctt ttgtatagac ctggacgagc
     4321 ctctggtgaa acagttattg agacgccaga tgctcaatgt cacaaggtca tgagttatca
     4381 attctcagca atctactata aaggtgatat gaatagctat cagttggcaa atgtagttag
     4441 tgagtatctt aaagatgctc agatttacca atacgcagat tatttaaata cccgtctgcg
     4501 ctttactcaa tttgaagttg aaaagtcttt gaacacaata agtgacatgt ttaatcttaa
     4561 taatgatggg atactaagtg tcgtgaaaaa ggcagaagaa cggcctggat atattcttcg
     4621 tctttataat ggtcaatatt tacaaagtat ttctccagag attacgttta gcaagcatcc
     4681 ttctgtagtg gagtttgtaa atttaaaaga ggagacgaaa gaaagtttag atattgctaa
     4741 tgaaaagatt ttattaggag atataggaca taataaattt attaccttgt atgtggagtt
     4801 ctaacagggg gagaaatcat tgaatgactt attatttcta gattctccaa tgcatgagaa
     4861 gatttggggt ggcaatcgtc tcaattctga atttggatat gatattccca gtgataagac
     4921 tggggaattt tgggctattt cagcccatcc taatggggtg tcgattgttt ctaatggtgt
     4981 atttaaagga caaagtttag ctaacatata tgcgaatcac gaagaattat ttggtgagag
     5041 tgaaagcaac gtttttcctc tcctcaccaa aattcttgat gcgaatgaat agctaagtgt
     5101 tcaagtacac ccagatgatg cctatgcttt gtctcacgaa ggtgaacttg ggaaaacgga
     5161 gtgctggtat attctgtctg ctgaagaaaa ttcagaaatt atttacggtc ataatgctcg
     5221 gagtagagaa gaacttcaac agatgattga aaataaatcc tgggataagc tgctacaacg
     5281 agttaaagtt aaggcaggag atttcttcta tgttccaagt ggtacaatgc atgccattgg
     5341 tcctggaatc atgattcttg aaacgcaaca atcgagtgat acaacgtata gaatctatga
     5401 ctttgataga gccgattcta gtggtcaaca acgtgacttg catattcacc agtctctaga
     5461 cgttttaagt ttcggaactc cgaataattc tcatcctgat aaaattgttg ttgagagctt
     5521 tgagaatacc attctcgttt cagctgaatt tttcacagtc tatcattgga aaattagtgg
     5581 tagagttcac tttaaaaatt tgctccgtac ttgctagtat ctgttatatc tggtgaagga
     5641 aaaattattc ttgattctgt tcaatataat ttagacttgt tcggtaactc aaaagtggta
     5701 tactaaagct atggaaacaa catacgaaaa agccttaaaa ttaaactcag agcatttcaa
     5761 attgttgata gg
//
LOCUS       DATUCG010000134         5724 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.136, whole genome shotgun sequence.
ACCESSION   DATUCG010000134 DATUCG010000000
VERSION     DATUCG010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5724
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.136"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(50..2668)
                     /gene="alaS"
                     /locus_tag="VCA20_002070"
     CDS             complement(50..2668)
                     /gene="alaS"
                     /locus_tag="VCA20_002070"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEP1835817.1"
                     /translation="MKNLSSAQIRQMWLDFWKSKGHSVEPSANLVPVNDPTLLWINSG
                     VATLKKYFDGSVIPDNPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAIEWGFELLTSPEWFALPKDKLYMTYYPDDVDSYNRWIALGVEPSHLIPLEDNFWEI
                     GAGPSGPDTEIFFDRGTDFDPEKIGIRLLEEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPNKNIDTGAGLERLAAIFQGAKTNFETDLFLPIIREVEKISGKTYDQDGDNMS
                     FKVIADHIRALSFAIGDGALPGNEGRGYVLRRLLRRASMHGQRLGITEPFLYKLVETV
                     GNIMESYYPEVLEKRAFIEKIVKSEEESFARTIHTGSQFAEQLMDKLAAEGKSEIDGR
                     DIFKLYDTYGFQVELTEELAEHRGMTLDIAGFEAAMKEQQDRARASVVKGGSMGMQNE
                     TLAAITEGSTFVYGQTALEASLSVIVADNARTEAVSEGQALLVFDQTPFYAEMGGQVA
                     DHGFVKNAAGDIVATVIDVQKAPNGQPLHTVELSASISVGQTYTLEIETKRRKGVEKN
                     HTATHLLHAALHNIIGEHATQAGSLNEQDFLRFDFTHFEAVTTEELRRIEEEVNEQIW
                     NAIPVVTVETDIDTAKEMGAMALFGEKYGKEVRVVTIGDYSVELCGGTHVSNTAEIGI
                     FKILKEEGIGSGTRRIIAVTGREAFLAYRDQEDALKEVAATIKSPQIKEVPNKVESLA
                     QQVRDLQKENAALKEKAAAAAAGDVFKEVKDANGIRYIASQVQVSDAGALRTFADNWK
                     QKDYSDVLVLVAAIGDKVNVLVASKSSDVHAGSLIKVLAPIVAGRGGGKSDMAMAGGS
                     DSSAIQTLLNSVADNL"
     gene            complement(3057..3542)
                     /locus_tag="VCA20_002071"
     CDS             complement(3057..3542)
                     /locus_tag="VCA20_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HEP1835818.1"
                     /translation="MKQFQVKQRFTFGGEKFDIRDSFGNLTYQVEGSFLEIPKRFIVT
                     NSRGGEVCQITKKFLTFLPQFTIDMAEGHSFYLQKEFTFFKDRYTVENLGLILDGNIW
                     DLDFCLKRPDGMLVAEISKELFHLTSHYSVTVLEDSYADLVISLVVAIDYIEMMEDAS
                     T"
     gene            complement(3808..4809)
                     /gene="prsA"
                     /locus_tag="VCA20_002072"
     CDS             complement(3808..4809)
                     /gene="prsA"
                     /locus_tag="VCA20_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEP1835819.1"
                     /translation="MKQTKKILAGAVTLFAAVTLAACSNAADKDIITMKGNTITVSEF
                     YEKVKTNSQAQQVLLSMVISNVFENQYGDKVSAEEVNKEYDKKAEQLGASFNAALSSA
                     GLTEESYKEQIRTNKLVEYAVKQAAEKELTDENYKAAYDAYTPEVTARVIKLADEAKA
                     KEVLAAAQAEGADFAQLAKDNSMDTTTKDNGGEVKFDSTSTTVPAEVQKAVFALDAGQ
                     VGASVISSVDMKTYTTSYYVVKLDAKSEKSAKWEDYKDKLKEIILAQKQSDSSFVATV
                     LKEALQKANVKVKDSAFQNLLSQYVTTEESSSSSTKSSSSSTESSSSTTESSSSSGQ"
     gene            complement(4870..5589)
                     /locus_tag="VCA20_002073"
     CDS             complement(4870..5589)
                     /locus_tag="VCA20_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEP1835820.1"
                     /translation="MVESYSKNANHNMRRPVVKEEIVDFMRTRQAQNTGFLKELEEFA
                     HQENIPVIPHETVAYFRLLMQTLQPKRILEVGTAIGFSALLMADNSPHSKITTIDRNE
                     EMIGFAKENLAKYDYRQQIELLEGEAMDILPTLPDNTYDFVFMDSAKSKYIVFLPEVL
                     KKVKVGGLIILDDIFQGGDVAKDIKDIRRGQRTIYKGLQRLFDATLDNPDVTASLVSM
                     SDGLLMLRKNVENVDLKHIEI"
BASE COUNT         1645 a         1273 c         1092 g         1714 t
ORIGIN      
        1 taccgaaaaa ctcttctacc caatggataa aagagttttt cggtatagat tataagttgt
       61 cagctacgct gttcaagagg gtttggatag cagaggaatc gctaccaccg gccatggcca
      121 tgtctgactt accaccacca cgacctgcta cgattggcgc aaggactttg atgaggctac
      181 ctgcatgaac atcgcttgac ttgcttgcta caaggacatt gaccttgtca ccgatagccg
      241 caaccaacac caagacatct gagtagtctt tctgcttcca attgtcagcg aaggtacgga
      301 gggcacctgc atctgaaact tggacttggc tagcaatgta gcgaatgccg ttagcatctt
      361 tgacctcttt gaagacatca ccagcagcgg ctgccgctgc tttttctttc agagctgcat
      421 tttctttttg caggtcacga acttgttgtg caagactttc aaccttgttt ggcacttcct
      481 tgatctgtgg tgacttgatg gttgccgcta cttctttgag ggcatcttct tggtcacgat
      541 aggcaaggaa ggcttcacgg cctgttaccg caatgatacg gcgagtacct gaaccgatac
      601 cttcttcttt caaaatcttg aagataccga tttcagcggt gttgctaacg tgggtaccac
      661 cacagagctc cacagagtag tcaccgatag taacaacgcg gacttcttta ccgtattttt
      721 caccgaagag agccatggca cccatttcct tggctgtgtc aatatcggtt tcaactgtca
      781 caactggaat ggcattccaa atttgctcat tgacttcttc ttcaatacga cgaagttctt
      841 ccgtagtaac tgcctcaaaa tgcgtaaagt caaagcggag gaagtcttgc tcgttcaagg
      901 aaccagcctg tgttgcatgc tcgccgataa tattgtgaag agctgcatgg agcaagtgag
      961 tggctgtgtg gttcttctca acacccttac gacgttttgt ttcgatttca agtgtgtagg
     1021 tttgaccaac tgaaatactt gcggacagtt caactgtgtg caatggttga ccatttggtg
     1081 ctttctgcac atcgatgaca gtcgcaacaa tgtctccagc agcatttttc acaaaaccat
     1141 ggtctgcaac ctgaccaccc atttctgcat agaatggtgt ttggtcaaat actaggagag
     1201 cttgaccttc tgagactgct tctgtacgcg cattatcagc cacaatgaca gataagcttg
     1261 cttcaagagc tgtttggccg taaacaaagg tcgacccttc tgtgatagca gccagagttt
     1321 cattttgcat acccattgaa ccacctttga caactgaggc acgggcacgg tcttgttgct
     1381 ctttcatagc cgcttcaaag ccagcaatgt caagggtcat accacggtgc tcagctaatt
     1441 cttctgtcaa ttctacttgg aaaccatagg tatcatagag tttgaaaatg tcacgaccat
     1501 caatctcaga cttaccttct gctgccaatt tatccatcaa ttgctcagca aattgtgaac
     1561 ctgtatggat agtacgagca aatgactctt cttcactctt aacaatcttc tcaatgaagg
     1621 cacgtttttc aagcacttct ggatagtagc tttccatgat attgccaact gtttccacca
     1681 atttgtacaa gaatggctcc gtaattccta aacgttggcc atgcatagaa gcacgacgaa
     1741 ggagacgacg aaggacataa ccacgacctt catttccagg aagggcaccg tcaccaatag
     1801 caaaagaaag ggcacggata tggtctgcaa taaccttgaa gctcatgttg tcgccatctt
     1861 ggtcataggt cttaccagaa atcttttcta cttcacgaat aattggcaag aagaggtctg
     1921 tttcaaagtt ggtcttagcc ccttggaaaa tagctgccaa acgctccaaa ccagcgcccg
     1981 tatcaatgtt tttatttggc aattccttgt attcagaacg cggaacagct gggtctgcgt
     2041 tgaactggga caatacgata ttccaaatct caatgtaacg gtcattttca atatcttctt
     2101 ccaaaagacg gataccaatt ttttcaggat caaagtcagt accacggtcg aagaaaatct
     2161 ccgtatctgg accagaagga cctgcaccaa tttcccagaa gttatcttca agcggaatca
     2221 agtgacttgg ctcaacacca agggcaatcc agcggttgta tgaatccaca tcatctggat
     2281 agtaagtcat gtagagcttg tcttttggca aggcaaacca ttctgggctt gtcaagagct
     2341 caaagcccca ctcaatcgct tcatcacgga aataatcacc aatagagaag ttgccaagca
     2401 tttcaaacat ggtgtggtgg cgggctgtct taccaacgtt ttcaatatcg ttggtacgga
     2461 tggatttttg ggcgtttgta atccgtgggt tatcagggat gactgagcca tcgaaatatt
     2521 ttttaagagt tgccacacca gagttgatcc acaaaagagt tggatcgtta acagggacta
     2581 gatttgctga aggctcaaca gagtgaccct ttgatttcca gaaatccaac cacatttgac
     2641 gaatttgagc agaagataaa tttttcataa actaagatac caagcccgat tagaaggcga
     2701 ctgaaaaata ggaaactgac aggaaatctt catttcctag gcagtttatc ttttttccga
     2761 gccttccggg cgggttcaat ttaacgaaac agttaaatta aacatttcct ttcatatttg
     2821 ataatttagg ggaaagtccg tagcccgagt gcaactaaga tactaagggc gttaagcgga
     2881 caccgtcaaa ataggaaacc tgacgaagac gcttgcgtct aggaagtgtt tctctttttg
     2941 acaaagtccg tagcccgagt tcatttcaga ataaactcaa taccctttcc tttcataagt
     3001 gatagttagg cgggttcaat ttaacggacc agttaaactg aatgattatt tcacgattaa
     3061 gttgatgcat cttccatcat ttcgatgtag tcaattgcga ccactaagga aatgaccaaa
     3121 tctgcatagg agtcctccaa tacagtaacg gagtaatgag aagttaaatg aaagagttct
     3181 ttggagattt cagctacgag cataccatct ggccttttca agcaaaaatc caaatcccag
     3241 atatttccgt ctaggattaa acctagattt tccacagtat agcgatcctt gaaaaaagta
     3301 aattcctttt gcaagtagaa agaatgaccc tctgccatat caatggtaaa ctggggcagg
     3361 aaggtcaaaa acttcttagt aatctgacaa acttccccac ctctggaatt ggttacgata
     3421 aatcgttttg gaatttcgag aaaagatccc tccacttgat aagtaagatt cccaaaacta
     3481 tcacgaatat caaatttttc accaccaaag gtaaagcgct gtttcacttg aaattgcttc
     3541 atgataacct ccaaaaaaaa taacaagaaa tcaaacgaag ggcgaaaaac cgcggtacca
     3601 ccttcattca atgacttgtc attctcattt aagaaactgt atccacaagc aaaaaacttt
     3661 tatatataag ccacaaactg tgaatacagg ctctactatt caattgtaat attaagtagc
     3721 aaaatacttg gcttagatgg ctcgcaacaa ccgccaattt tctgaactaa ggtgtcaagt
     3781 accaattctt aataagtaaa tcgtcaatta ctgacctgat gaactagaag attctgttgt
     3841 agaagaagaa ctttcagtcg acgaagagct gctcttcgtt gaggaagaac tgctttcttc
     3901 tgttgtcaca tactgtgaaa gaagattttg gaaagcactg tctttaacct taacgtttgc
     3961 tttttggagt gcttctttca gcacagtagc aacaaatgat gaatcacttt gcttttgtgc
     4021 caaaataatt tctttcagct tatctttata atcttcccat ttagcagatt tttctgattt
     4081 agcatccaat tttacaacgt aatagctcgt tgtatacgtc ttcatatcta ccgaagaaat
     4141 caccgacgct ccgacttgac ctgcatccaa ggcaaatact gctttctgta cctctgctgg
     4201 aacagttgtt gaagttgaat cgaatttaac ttcgcctcca ttgtcttttg tagtagtatc
     4261 catggaattg tctttcgcca attgagcaaa atctgcacct tcagcttgtg cagcagctaa
     4321 aacttctttt gctttagctt cgtctgccaa cttgatgaca cgtgctgtta cttcaggtgt
     4381 ataggcgtca taagctgcct tgtagttttc atctgtcaat tctttctcag cagcttgttt
     4441 tacagcatac tcaaccaatt tattggtacg aatttgttcc ttataggatt cttctgtcaa
     4501 tcctgccgaa gaaagtgccg cattaaaaga agcaccaagt tgttctgcct tcttatcata
     4561 ttccttattt acttcttctg cagagacttt gtcaccatac tgattttcaa atacattact
     4621 gataaccatt gacagcaaaa cttgttgagc ttgtgagttt gtttttactt tctcataaaa
     4681 ctcagacaca gtaatcgtgt tacctttcat cgtaatgata tctttatcag ctgcattgga
     4741 acaagcagcg agtgttactg cagcaaagag tgttactgca cctgcgagaa tttttttagt
     4801 ttgtttcatt aaagactctc tccttttttc attaacccct ttattataac acaaaagctt
     4861 aaaaacttct taaatttcta tatgttttaa gtcaacattc tctacatttt tcctcaacat
     4921 aaggagaccg tcactcatgg aaacaagact agcagtcaca tctggattat ccaaagtcgc
     4981 atcgaataaa cgttgcaatc ccttataaat cgttcgctgc cctcttctga tatccttgat
     5041 gtccttggca acatctcctc cttggaagat gtcatccaaa atgattaaac cacctacttt
     5101 gacctttttc aagacttctg gtaaaaatac aatatatttt gatttagcag aatccatgaa
     5161 aacaaaatca tatgtgttat ctggtaaagt aggaagaata tccatagctt ctccttccag
     5221 caattcaatc tgctggcgat aatcatactt ggcaaggttt tccttagcaa atccaatcat
     5281 ttcttcattt cgatcaatcg tcgtaatctt cgagtgcggg ctattgtcag ccatcagtaa
     5341 cgcagagaag ccaatagcag ttccaacttc taaaatacgt ttaggttgga gtgtctgcat
     5401 cagcaagcgg aaataagcta ccgtttcatg agggataact ggaatgtttt cttgatgagc
     5461 aaactcttcc agttctttca aaaaccctgt attttgcgcc tgacgtgttc gcataaaatc
     5521 cacaatttct tccttgacaa ccggacggcg catattgtga tttgcgtttt tactatatga
     5581 ttcgaccata gaatccatta tatcataagc cgacaagatt ttcttcctca acacactaaa
     5641 aggtaggttt cccccaatgt cattaagtta actaacctat cacattttgt ggtgggttat
     5701 ttttatgtta cactaaaaat aaaa
//
LOCUS       DATUCG010000135         5667 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.137, whole genome shotgun sequence.
ACCESSION   DATUCG010000135 DATUCG010000000
VERSION     DATUCG010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5667
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.137"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            263..>5667
                     /locus_tag="VCA20_002074"
     CDS             263..>5667
                     /locus_tag="VCA20_002074"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019202.1,HMM:TIGR01168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HEP1835821.1"
                     /translation="MSYKDMFRKEQRFSFRKFSFGLASAVIANVILGGAIANSPVVHA
                     NTVTEAQATAVSERIASIPYTVKIEDQTGKVVETKVKMVSVYTVETIATATDYLTADL
                     VPEGYAIVSGLGEVTVTEKVDNIFTVKVDKIAPEAVATTTTAESATSETSTLIAPSDA
                     TTVASDVTKPATPEPASLTATQPIPVTSEIAEAEVAPTTSEESAKPAERTVYLSYITQ
                     YVNEDRQVVDSKGHLVAVKTTDETAKTQVTVSVSKDVPAGYELVQAKPEITLQLVEKQ
                     INVLAFVVTKKSEEEEIAESQLSNRDVLMRLSLEANLLADEALRQVAKEQAGNTELAT
                     AANKAKEIATNDVLGSETKTEEELGEQVKTVRASIENLAAEMLKVDEDGILTAQLNTT
                     QVSRPANYQEIGTEAGAFHPGRPTLTRYLFVFGTTEGTTESDASGTLNAAPVANKDDA
                     TRSLVPAGTITNIVYNDGISNLANGTLGSTLTMDTDGFLDGTFNHRAGGTYTRKLLVT
                     TDTGEQRSNDFTVYAYTDTASSDHPVAKLEGETLTQDEVFAKMAIVTSTSGEVTAGGK
                     AFPNTGKNIPTVPADEYTRTILGYRTVTEDAFGEVTPGAYVEQTDLAQFPTNTDYQVK
                     VKTTNVYGQTIYNWIPVSIYKLAEISRDTVDQYTDNPVPIHTIVELGSTGTIGTVKLA
                     GTLPEGFDIKNFSLKVGEAEKLAQRNLEFVKATNVTDGLLGTIQAKNGGKVEYTGSGN
                     IDYRFVYSYMEGTETKTVDLTYTILFTDRTAPVMTPAAEYVRVVGENYTISVPGSDNA
                     FLSPNKINGTLSVLKSDEVGKVSPSLGTNTDITSELDPSGKDISGGSDDQGGNSTKFN
                     IKITGTAPATAGTGSYKLRVGEDNFTASTRYKEKLKQEGKVPENVSYTDIKVTFVDKV
                     KPITAIPVADQSHLTPTEKKAVIDAVKEANPNNAKLLALPESAFTVNSDGSVSVNYSG
                     DNVDGATDTIKNATTKLADTQNKAKAEIDAKLAENKKAIEAKRDAAIAEIDDNIDLTD
                     VQKKVAKNAIAAVADNALKDLDTKATEAKENIDKATTVSEIDDAKVKGEFDLDGAKEA
                     GEKAINWEKEKEEAKAAVEDQSTDALEIINNNPNLSYEEKKAYFDAIDASKKAAIEKI
                     DEAKDSVNITAAIDKAAKANETEVLKAAKQDALNKLEKDAAKAKEDVADNSNLTKEQL
                     EKALEDIQNDLDKAKATVNKAATQAAVQKGEDKGVAAIAKDVLDATKQNVLNQLEKDA
                     ARAKEDVAKNPNLTKEQLEKALEGIQNDLDKAKTAINEATTSAAVQEKMNKGVTALAK
                     DVLDAAKQDAKNKIAKEAELAKTAIDASPNLTSEEKDVAKKAVEEAAKVATDAIDKAT
                     NLSDVQTAEDNGVKAIDAEELKAAQKDAKNKIAKEAESAKKAIVNLTPEEKESAKKAI
                     DTDAQAATAAIDKASTPDAVQVEEDKGVAAINLITAKADEIKKLEDKQAEAEKAIDAS
                     TMTNEEKAIAKKALQDVVDKGKAELEDVARVATNEIHKATTAEDAKAAALAGEKSLTD
                     AGKKVRDAVELAKDKELAKKAIRTEVEEATKAVEKLAEDTRKAIKENPNLSDEDKQAE
                     IKKLTDVVAKTLETIRDNATTAMQEAEKTQALAALKKVKETQKIADKAAIDRLTILVK
                     EGELEAAKRDVLNQLEKDAARAKEDIAKNPNLTKEQVDKALEDVQKDFDKAKTAINEA
                     ATPDAVQAEMDKGVAALAKDVLDAAKQDAKNKIAKDAAAAKEAIATNPNLTDAEKESA
                     KKAVDADAKVATDAIDKA"
BASE COUNT         2068 a         1035 c         1258 g         1306 t
ORIGIN      
        1 gatagatttt cagtaaattt ttcaaaaaaa cctcaaaaat aacagatttt tccttgtatc
       61 tttgaggcat aagtagtata atggtgacgg tatttaagta gaaattttat atactctgga
      121 tgaaaacatt ctgtctactt taaaataaat aatctactga gtatccttct gctaagtttt
      181 taaagcagga ggtgtgtttt tgtacatggt gttacaagaa tcagaaatga tggtgtttgc
      241 cagtaaaata taggaggata tcatgtctta taaagatatg ttcagaaaag aacaacgttt
      301 ttcttttcgt aaatttagct ttggtctagc ttcggcagtc attgcaaacg ttattttggg
      361 aggagcaatc gcaaacagcc cagttgttca tgctaacaca gtgacagaag cacaggcaac
      421 tgcagtgagt gagcggatag catccatacc atacacagtt aaaatcgagg atcaaacagg
      481 taaggttgtt gaaacgaaag tgaaaatggt ttcagtatat acagttgaga ctattgcaac
      541 agcaacagat tacttgactg ctgaccttgt gccagaaggc tatgccattg tatcaggatt
      601 aggtgaagtg accgttactg aaaaagtaga caatatcttc acagttaaag tagacaaaat
      661 tgcaccagaa gcagtagcaa caactacgac agctgagtca gctacatcag aaacatcaac
      721 attgattgcc ccgtctgacg caacaacagt agcatcagat gtgactaagc cagcaactcc
      781 tgagccagca agtttaacag ctacgcaacc aataccagtg acgtcagaaa tagctgaggc
      841 tgaggtagca ccaactacat cagaagaatc agcgaaacca gcagagcgta cagtttatct
      901 tagttacatc acacagtatg tcaatgaaga cagacaagtt gttgatagca agggtcacct
      961 tgtagctgta aaaacaactg atgaaacagc aaaaacacaa gtaaccgttt ctgtatctaa
     1021 agacgtacca gcaggctacg aattggtaca agccaagcct gaaattactc ttcaattggt
     1081 tgaaaaacaa atcaacgttt tggcttttgt tgtaactaaa aaatctgaag aagaggaaat
     1141 tgctgagtca caattgtcaa accgtgatgt cttgatgcga ttgtctttag aagcaaactt
     1201 gcttgcggac gaggcactac gccaagtagc gaaagagcaa gctggtaaca ctgaacttgc
     1261 aacagcagct aacaaagcca aagaaattgc aaccaatgac gtcttaggaa gtgagactaa
     1321 aacagaagaa gaactcgggg aacaagtcaa aacagttcgc gcaagcatcg aaaaccttgc
     1381 agctgaaatg ttgaaagtcg acgaagatgg aatcttgacg gcacagttga atacaactca
     1441 agtgtcacgc ccagctaact atcaagaaat cggtactgag gcaggtgctt tccacccagg
     1501 tagaccgact cttacgagat atctctttgt cttcggtact acagaaggta ccactgaaag
     1561 tgatgctagt ggaactctaa atgcggctcc agttgcgaat aaggacgatg caacacgatc
     1621 gcttgtccca gctggaacaa tcacaaatat tgtctataat gacggtatta gtaaccttgc
     1681 caatggtact ttgggaagta ccttgacaat ggatacggat ggcttccttg acggtacctt
     1741 taaccataga gcaggtggaa catacactcg taagttactt gtgactactg atacaggtga
     1801 acaaagaagt aatgatttca cagtctatgc ctacactgat acggcctcta gcgatcatcc
     1861 ggttgcaaaa ttggaggggg aaacattaac tcaggatgaa gtatttgcta agatggcaat
     1921 tgttacgagt acttcaggag aggtcactgc aggagggaaa gctttcccaa atactggaaa
     1981 aaatattcca acagttccag ctgacgagta cactcgtact atcctaggct accgtactgt
     2041 aactgaagac gccttcggcg aagttacacc aggtgcctat gttgaacaaa ctgaccttgc
     2101 tcaattccca actaacacag actaccaagt taaagttaaa acaactaatg tctatggtca
     2161 aacaatctat aactggattc ctgttagcat ttataagttg gcagagattt ccagagatac
     2221 tgtcgaccaa tacacggata accctgttcc aattcacact attgttgagc ttggttctac
     2281 aggtactatc gggacagtta aactggcggg aactctccca gaaggctttg atattaaaaa
     2341 tttcagcctt aaagtaggtg aggcagaaaa actcgctcag cgtaatctgg agtttgtaaa
     2401 agcaactaat gtcactgatg gtcttttagg tactattcag gctaaaaatg gtggtaaagt
     2461 agagtataca ggttcaggta acatagacta tagatttgtt tactcttaca tggaaggaac
     2521 ggagactaag actgtcgatc ttacttatac tatcttgttt acagatagaa ctgctccggt
     2581 gatgacacct gccgctgaat atgttcgcgt tgttggtgaa aactatacta tcagtgtccc
     2641 tggctcagac aacgccttcc tcagtccaaa caagattaat ggtactttgt cggttcttaa
     2701 atcggatgag gttggtaaag tttctccaag tttaggaaca aacacggata ttacctctga
     2761 gttggatcct agtggaaaag atatttctgg aggaagtgat gatcaaggcg gtaactctac
     2821 taaatttaat ataaaaatta caggaactgc accagctaca gcaggcacag gaagctataa
     2881 acttcgtgta ggtgaagata actttacagc gagtacacgc tataaagaaa aattgaaaca
     2941 agaaggtaag gttccggaaa atgtaagtta tacagatata aaagttacct tcgttgacaa
     3001 ggtgaaacca atcacagcaa ttccagtcgc tgaccaatca cacttgacac ctactgaaaa
     3061 gaaagcagtt attgatgcag ttaaagaagc taatccaaat aatgctaagt tactggcact
     3121 tcctgagtca gctttcacag tgaattctga tggtagtgta tcggttaact atagtggtga
     3181 taatgttgat ggtgcgacag acactattaa gaatgctacc acaaaattgg cagatacaca
     3241 gaataaagca aaagctgaaa tcgacgctaa attagctgaa aataaaaaag ctatcgaagc
     3301 aaaacgggat gcagcaattg ctgaaattga tgataacatt gacttgacag atgttcagaa
     3361 gaaagttgct aagaatgcca ttgctgcagt tgctgataat gctttgaaag acttagacac
     3421 caaggcgaca gaagcaaaag aaaacattga taaagcaaca actgtttcgg aaatcgatga
     3481 cgctaaggtt aaaggtgaat ttgatttgga tggtgccaaa gaagcaggcg aaaaagctat
     3541 taactgggag aaggaaaagg aagaagcaaa agcagcagtt gaagatcaat cgacggatgc
     3601 attggaaatc attaacaata atccaaactt gtcatacgaa gagaaaaaag catattttga
     3661 tgctattgac gcatctaaga aagcagcaat tgaaaaaatt gatgaagcta aagactctgt
     3721 caacattacg gcagcaattg ataaagcagc caaagcaaac gaaacagaag ttcttaaagc
     3781 cgctaagcaa gatgccttga acaagcttga gaaagatgca gccaaagcta aagaagatgt
     3841 tgcagacaat tcaaacttga ctaaagaaca attagaaaaa gcacttgaag atattcaaaa
     3901 cgatcttgac aaggctaaag ctactgttaa taaagctgca acccaagctg cagttcagaa
     3961 aggagaggat aagggagtag cggccatcgc caaagatgtt cttgatgcca ctaaacaaaa
     4021 tgtcttaaac cagcttgaga aagatgcagc cagagccaaa gaggatgttg caaaaaatcc
     4081 aaacttaact aaagaacagt tagaaaaagc acttgaaggc attcaaaacg atcttgacaa
     4141 ggctaaaact gctattaacg aagccacaac ttcagccgca gttcaggaaa aaatgaataa
     4201 aggtgtaact gcacttgcta aagatgttct tgacgcagcg aaacaagatg ctaagaacaa
     4261 gattgccaaa gaagctgaat tagctaagac tgcgattgat gccagtccaa acttgacttc
     4321 agaagagaaa gatgtagcta agaaagcggt agaggaagca gctaaggtag cgacagatgc
     4381 tattgataag gcaactaatc tatcagacgt tcaaacagcg gaagacaatg gagtgaaggc
     4441 tattgatgca gaagagctta aagctgctca aaaagatgct aagaacaaga ttgccaaaga
     4501 agcagaatca gctaagaaag cgattgtaaa cttgactcca gaagagaagg aatcagctaa
     4561 gaaagcaata gataccgatg ctcaagccgc gacagccgct attgataaag catcaactcc
     4621 agatgcagtt caagtagaag aggacaaagg tgtagcagct atcaatttga ttactgccaa
     4681 ggcagatgaa atcaaaaagc tcgaagataa gcaagcagaa gcagaaaaag ctatcgatgc
     4741 gtcaactatg actaatgagg agaaagcaat cgctaagaag gctcttcaag atgttgtaga
     4801 taaaggaaaa gcagagcttg aagacgtagc tagggtagca acaaatgaga ttcataaagc
     4861 tactactgca gaagacgcga aagcggctgc acttgctggc gaaaagagct tgaccgatgc
     4921 gggtaaaaaa gttagagatg cagttgaatt ggctaaggat aaagaattag ctaagaaagc
     4981 aatccggacg gaagtagaag aagctactaa agcagtagag aagcttgcag aagatacgcg
     5041 caaagctatc aaggaaaatc caaacttgtc agatgaagat aagcaagcgg aaattaaaaa
     5101 gctaactgac gttgtggcaa aaactttaga aaccattcgt gacaatgcaa ctacggctat
     5161 gcaagaagca gaaaaaacac aagccctagc agctcttaaa aaagttaaag aaacacagaa
     5221 aattgcagat aaagctgcga ttgataggtt gactatactt gtaaaagagg gtgagcttga
     5281 agctgctaaa cgagatgttt taaatcagct cgagaaagat gcagccagag ctaaagagga
     5341 tattgcaaaa aatccaaact tgactaaaga acaagtagac aaagcacttg aagatgttca
     5401 aaaagatttt gacaaggcta aaactgcaat taatgaagct gcaaccccag acgcagttca
     5461 ggcagaaatg gataaagggg tagctgcact tgctaaagat gttcttgatg cagcgaaaca
     5521 agatgcgaag aataagattg ctaaagatgc agccgcagct aaagaagcca ttgcaaccaa
     5581 tccaaacttg acagatgcag agaaggaatc agctaagaaa gcggtagatg ctgatgctaa
     5641 agttgcgaca gatgcaattg ataaggc
//
LOCUS       DATUCG010000136         5636 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.138, whole genome shotgun sequence.
ACCESSION   DATUCG010000136 DATUCG010000000
VERSION     DATUCG010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5636
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.138"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(10..903)
                     /locus_tag="VCA20_002075"
     CDS             complement(10..903)
                     /locus_tag="VCA20_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEP1835822.1"
                     /translation="MEVRVEVGGIRLDKALSDLTDLSRSVANEQIKAGQVLVNGQPKK
                     AKYSVQVGDVLTYQIPEVEEIDYVAEDIPLEIVFQDEDVAVVNKTQGMVVHPSAGHTS
                     GTLVNALLYHVKDLSGINGVLRPGIVHRIDKDTSGLLMIAKNDDAHTKLAAELKDKKS
                     LRKYWAIVHGNLPNDRGVIEAPIGRSEKDRKKQAVTAKGKEAVTRFQVLERFGDYTLV
                     ELTLETGRTHQIRVHMAYIGHPVAGDEAYGPRKTLKGHGQFLHARTLGFTHPRTGEVV
                     EFTVEAPAIFQEALEKLRKAE"
     gene            complement(887..1351)
                     /locus_tag="VCA20_002076"
     CDS             complement(887..1351)
                     /locus_tag="VCA20_002076"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEP1835823.1"
                     /translation="MRKIGFPFLMIVLIGLDQFVKAWTVANIELDTVTEFIPGLMSLA
                     YLRNYGAAWSILQNQQWFFTIMTIVAVTGLVWYYIKQIKGNIWTLFSLSLMIAGALGN
                     FIDRLRLGYVVDMFHLDFISFPVFNVADVCLTVGVGILFICIMKEESNGSKS"
     gene            complement(1344..2258)
                     /locus_tag="VCA20_002077"
     CDS             complement(1344..2258)
                     /locus_tag="VCA20_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEP1835824.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISIRDLEKELG
                     FQIFSRTSSGTFLTQKGMEFYEKAQALVKGFDQFEHLYLQPEEGEKTFSISSQHYDFL
                     PPLITEFSRSHPQYPHFRIFESTTVQILDEVAQGYSELGIIYLNERNTKGIMQKLDKL
                     HLQAVDLSAFQTHIYLREDHPLTKKVEIDSADLVGLPTVRFTQEKEAYLYYSENLIDT
                     SDSSVVFDVTDRATLNGILERTDAYATGSGYLDDESVNGITVIPLKGKVDNRMVYVKR
                     ANDELSSCAKDFIETMQAYFDKKKDENA"
     gene            complement(2341..2481)
                     /locus_tag="VCA20_002078"
     CDS             complement(2341..2481)
                     /locus_tag="VCA20_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEP1835825.1"
                     /translation="MGPNLAKLVIDDLANRNSKAFQIAVEEEKLGTWKLYKKLVLKNR
                     TG"
     gene            complement(2691..2984)
                     /gene="rpmA"
                     /locus_tag="VCA20_002079"
     CDS             complement(2691..2984)
                     /gene="rpmA"
                     /locus_tag="VCA20_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEP1835826.1"
                     /translation="MLNLNLANLQFMAHKKGGGSTSNGRDSQAKRLGAKAADGQTVSG
                     GSILYRQRGTKIYPGANVGRGGDDTLYAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            complement(3031..3345)
                     /gene="rplU"
                     /locus_tag="VCA20_002080"
     CDS             complement(3031..3345)
                     /gene="rplU"
                     /locus_tag="VCA20_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEP1835827.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAIYVEKLNVEAGQEVTFEEVVLVG
                     GEKTVVGTPLVAGATVVGTVEKQGKQKKVVTFKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     misc_feature    complement(3381..3469)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            complement(3536..4861)
                     /gene="brnQ"
                     /locus_tag="VCA20_002081"
     CDS             complement(3536..4861)
                     /gene="brnQ"
                     /locus_tag="VCA20_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEP1835828.1"
                     /translation="MKKGALTGLLLFGMFFGAGNLIFPPALGVLAGENFWPAILGFVV
                     SGVGIAVVALIVGTLNPKGYVHDISRKISPTFATIYLVALYLAIGPFFAIPRTATTSF
                     EIGIAPLLSDANLGLWLLGFTALYFVAAYLIALNPSQILNSIGRILTPVFAVLIVILV
                     VLGIFKYGSTSPLPASEAYSAGQAFGTGFIEGYNTLDALAAVAFSVVAVNTLKQLGFS
                     SKKEYVSTIWSVGLIVALAFSALYVGLAFLGNHFPVPADVLASDTNKGVYILSQATQA
                     IFGPGAQIFLAAMVIVTCFTTTAGLIVSSGEFFAERFPRFSYKVYATIFTLIGFGIAN
                     LGLNNIITFSVPVLLVLYPITICIVLITIVNKFVPLSKYGMQLTVGLVTALSLAEVLA
                     GQFGWAAVSKIISALPLAGQSLAWLLPALVGIVLSLFLPNKQESEVFEM"
     gene            complement(5146..>5636)
                     /locus_tag="VCA20_002082"
     CDS             complement(5146..>5636)
                     /locus_tag="VCA20_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEP1835829.1"
                     /translation="GKSYDTTDNKPTEIVTEDGSRYVLIPSKTIGTENGTVEGGKTTE
                     VTYVYRKVTPAKKVVTNHVDEDGNPIAPQEEGTTPNKSIPGYEFTGKTITDKDGNTTH
                     IYRKLSNKTTTPEKKTPAKPQTGKAQLPNTGEASSVAGALGTAMLVATLAFARKRRRN
                     ED"
BASE COUNT         1730 a         1282 c         1023 g         1601 t
ORIGIN      
        1 ttcatactat tattcagcct tgcgcagttt ttcaagtgcc tcctgaaaaa tagctggcgc
       61 ttctaccgta aattcaacaa cctcaccagt tctcggatgg gtaaatccaa gcgttctggc
      121 atgaagaaat tggccatgcc cttttagtgt ttttcttggg ccgtaagctt catcacccgc
      181 aaccggatga ccaatataag ccatgtggac acgaatttgg tgagttcgtc ctgtttccaa
      241 ggtcaattct accaaagtgt agtcaccaaa acgctccaaa acctgaaaac gtgtcaccgc
      301 ttctttccct ttggcagtca ccgcttgttt cttgcggtct ttttcagaac gaccaatcgg
      361 cgcctcaatt actccgcgat cgtttggtag attgccgtgt acaatggccc aatacttacg
      421 aagggatttc ttatctttca attcagcagc aagctttgta tgggcatcat cgtttttagc
      481 aatcatgagc aagccagatg tgtccttatc aatccgatgg acaatacctg gtcgcaagac
      541 accattgata cctgacaaat cttttacgtg ataaaggaga gcattaacta gcgttccaga
      601 cgtgtgacca gccgacggat gaacgaccat cccttgagtt ttatttacaa cagcaacatc
      661 ctcgtcctga aagacaatct ctaaaggaat atcctcagca acatagtcga tttcttctac
      721 ttctggaatt tgataggtta ggacatcccc aacctgcaca ctatatttag cttttttagg
      781 ctgtccattg accaaaacct gaccagcctt aatctgctca tttgcaaccg aacgagacaa
      841 atccgtcaaa tccgacaatg ccttatccaa acgaatacca ccaacctcaa ctcttacttc
      901 cattactctc ttctttcata atacaaataa ataaaatacc tacaccaacc gtcagacaaa
      961 catctgctac attaaataca gggaaactaa taaaatccaa gtgaaacatg tccaccacat
     1021 atcccaatct aagacgatca ataaaattcc caagagcacc agcaatcatc aaagacagac
     1081 tgaataaagt ccagatattg cccttgatct gtttgatata gtaccacact aggcctgtca
     1141 cagcaacaat tgtcattatc gtgaaaaacc actgctgatt ttgtaaaatc gaccaagctg
     1201 caccataatt tcttagataa gctaagctca tcaatcctgg tataaattcc gttaccgtat
     1261 ctaattcaat attggcaact gtccaagctt ttacaaactg gtccaaacca attaacacta
     1321 tcatcaaaaa tggaaatcca attttacgca ttttcatcct tcttcttatc aaaatatgcc
     1381 tgcatggttt caataaagtc tttggcacaa gaactaagtt catcattagc tcgtttaacg
     1441 tagaccatgc gattgtctac ctttcctttg agaggaatta cggtaatgcc attgacactc
     1501 tcgtcatcaa gatagcccga accagttgca taggcatccg tacgctccaa aattccattc
     1561 aaagtcgcac ggtctgtcac atcaaacaca accgaggaat ccgacgtatc aatcaagttc
     1621 tcggaataat aaagataagc ctctttttct tgagtgaacc gtacggtagg tagacctacc
     1681 aaatctgccg aatcaatctc aactttcttt gtcaaaggat gatcttcacg aaggtagata
     1741 tgagtctgaa atgcagacaa atctacagct tgcaagtgaa gtttatccaa tttctgcatg
     1801 attccttttg tatttctctc gttcaaataa ataataccaa gctcgctata accttgagca
     1861 acctcatcta aaatctgtac tgtagtcgat tcaaaaatgc gaaaatgagg gtactgaggg
     1921 tggctacgag agaactccgt aatcaatggg ggaagaaaat cataatgctg acttgaaatt
     1981 gagaaagtct tctcaccttc ctcaggttgc aaatagagat gctcaaactg atcaaatccc
     2041 ttaactaaag cctgagcttt ctcatagaat tccatccctt tttgtgtcaa aaaagtccct
     2101 gaactagttc ggctaaaaat ttgaaaacct aactcttttt ccaaatcacg aatggaaatg
     2161 gacaaactag gctgggaaac gtacattttc tctgctgctt cccgaaaagt accactgttg
     2221 gcaatggcta caacgtagcg taattgttga atattcatac ctgatacctc ttaaaactgc
     2281 ttctaccatt atatcaaaaa aagcaagaat ttgctgaact cttgcgtttt cgcaaataga
     2341 ctagcccgtc ctgttcttca aaactaattt cttgtataac ttccaggttc ccaatttctc
     2401 ttcttcaact gcaatttgaa aggctttgga atttctgttc gccaaatcat cgatgactaa
     2461 ttttgctaag ttgggaccca taccacttcc tctgaacatt tcatctacta ctgaacgtag
     2521 aacttatagg cagcgcaaca gtgcagatgg caaaatattt ttatcctata gagaagatat
     2581 tgccgtctta aatcacttct atcgtacact tttaaaaata gcaaaatcaa attacaaaaa
     2641 agactctccg aaaacaactc ggaaagccta ttgtaaagcc attttcagac ttattttgcg
     2701 attgggtaaa cagatacttg tttcttatcg cgacctttac gttcgaagcg tacaacgcct
     2761 tctactttag cgtaaagagt gtcatctcca ccacgaccta cgttagctcc tgggtagatt
     2821 tttgtaccac gttggcggta aaggattgaa ccacctgata cagtttggcc gtcagcagct
     2881 ttcgcaccaa ggcgtttcgc ttgtgaatca cgaccgtttg acgttgaacc tccacctttt
     2941 ttgtgggcca taaattgcaa gttagcaaga ttcaagttta acatatgtta ttcctccgaa
     3001 tttctgtaat gattgcttca agctacgcga ttaagcgttg atagcgttga taacaacttt
     3061 tgtgtaaggt tgacggtgac cttgtttgcg gtggctacct tttttaggtt tgtacttgaa
     3121 ggtaacaact tttttctgtt taccttgttt ttcaacagta ccaacaacag tagcgcctgc
     3181 tacgagtgga gtacccacaa cagttttctc accaccaaca agaactactt cttcgaaagt
     3241 aacttcttga cctgcttcaa cgttcaattt ttcaacgtag atagcttgac cgacttcaac
     3301 tttaacttgt ttgccgccag ttttaatgat tgcatatgtg ctcattatgc acctcctatg
     3361 atttttgggt cttgcccgaa tttgatgtga agactcgcct agtaccgtgg gacgaaccac
     3421 ttactctact ctcaacgaaa gaacggcatt cgtgcggttg cacaggatat gtgcatagtc
     3481 aacagtccta ttataacaaa aacacctgca aatgcaagtg ttttgtatca ttgttttaca
     3541 tttcaaaaac ttcgctctcc tgcttgtttg gcaagaaaag tgaaagaacg attccgacaa
     3601 gagctggtaa taaccatgct aatgattgtc ctgccaatgg caaagctgag ataatttttg
     3661 aaacagctgc ccaaccaaat tgaccagcca aaacttctgc caatgacaaa gctgtcacta
     3721 gtcccacagt taattgcata ccatattttg aaagcggtac aaatttgttg acaatggtaa
     3781 ttaaaacaat acagatggtg attggataga gaaccaaaag aactggaact gagaaagtaa
     3841 tgatattatt caaaccaagg ttggcaatcc caaaaccaat caaagtaaag atagtcgcat
     3901 atactttgta gctaaaacgt gggaaacgtt cagcgaaaaa ttcacctgaa gaaacaatca
     3961 agccagccgt tgtcgtaaag caagtaacga taaccatggc agctaagaaa atttgtgcac
     4021 ctggtccaaa aatagcctgt gttgcttgcg aaagaatata aacaccctta ttggtatcag
     4081 aagccaaaac atcagcagga actgggaaat gatttccaag gaaggccaaa ccaacataga
     4141 gcgccgagaa agccaaggct actatcagac caactgacca aatggtagat acatattcct
     4201 ttttgcttga gaaaccaagt tgtttcaagg tattgacagc taccacacta aaagcaaccg
     4261 ccgcaagagc atcaagggtg ttgtatcctt caataaatcc tgtaccaaat gcctgtcctg
     4321 ctgagtaagc ttctgaagct ggtaacggac tggtcgaacc atacttgaag atacccagca
     4381 ccaccaaaat cacaatcaga acggcaaaga ctggtgtcag aatacgtccg atactattca
     4441 agatttgtga cggattgagg gcaatcagat aagctgccac aaagtaaaga gctgtaaagc
     4501 ctaacaacca gagaccaaga ttcgcatcgc tcaacaatgg cgcaatacca atttcaaagg
     4561 acgttgtagc tgtacgcgga atagcaaaga aaggaccgat agccaaatac aaggcaacaa
     4621 gataaatcgt tgcaaaagtc ggtgaaatct tacgagaaat atcatgtaca taaccttttg
     4681 ggtttaaggt accgacaatc aaggcaacga cagcaatacc gacacccgat acaacaaatc
     4741 ctaaaatagc tggccagaaa ttctcaccag ctaagacacc aagtgcaggt gggaaaatca
     4801 agtttcctgc accaaaaaac ataccgaaca gaagtaaacc tgttagcgcc ccttttttca
     4861 taataactcc tttgtatttt tctgaagtct tattatatca tacaaattcg atttttccga
     4921 acgattcgaa ctcgaccgaa taaaaaaaga gctcgtctcc cacccccaaa cagctccaac
     4981 agtcagagta gtgacagttg gaggttggaa atggagcgaa ctctaagatg atgtagctct
     5041 ggactttttt cccagcctca ttttttccga aaggtaatcc tgcatgttac gtacaattct
     5101 tatactttct atatggggga ataaagtctg tataaagaat tttgactaat cttcgttacg
     5161 acgacgtttt cttgcaaacg caagtgttgc gacaagcatt gctgtaccaa gcgcacctgc
     5221 cacagatgaa gcctcaccag tatttggcaa ttgagcttta cctgtttgag gttttgcagg
     5281 agttttcttc tctggtgtcg ttgttttatt agaaagttta cgataaatat gcgttgtgtt
     5341 gccatcttta tcggtaattg tcttaccagt gaattcataa cccggaattg acttgttcgg
     5401 tgttgttcct tcttcttgcg gtgcaattgg gttaccgtct tcgtcaacgt ggtttgtgac
     5461 aactttctta gctggagtca ctttacggta aacgtaggtt acttcagttg tcttaccacc
     5521 ttcaactgta ccgttttctg taccaattgt cttagacgga atcaagacat aacgtgaacc
     5581 atcttctgta acaatctctg ttggcttgtt atcagttgtg tcgtatgatt taccag
//
LOCUS       DATUCG010000137         5563 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.139, whole genome shotgun sequence.
ACCESSION   DATUCG010000137 DATUCG010000000
VERSION     DATUCG010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5563
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.139"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            <1..166
                     /locus_tag="VCA20_002083"
     CDS             <1..166
                     /locus_tag="VCA20_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEP1835830.1"
                     /translation="AEQGHRLLPLPPYSPEYNPIKKTWAHIKKHLRRVLPNCDTFLEA
                     LLSCSCFSRL"
     gene            165..719
                     /locus_tag="VCA20_002084"
                     /pseudo
     CDS             165..719
                     /locus_tag="VCA20_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735765.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
     gene            706..1382
                     /locus_tag="VCA20_002085"
                     /pseudo
     CDS             706..1382
                     /locus_tag="VCA20_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637824.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
     gene            complement(1431..1913)
                     /locus_tag="VCA20_002086"
     CDS             complement(1431..1913)
                     /locus_tag="VCA20_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEP1835831.1"
                     /translation="MTKKPVLLATICYIDNGKEFLLLHRNKKENDVHQGKWIGVGGKL
                     EPGETPQACAIREIFEETGLTVTKHALKGVITFPDFTPNTDWYTYVFKITGFEGSLID
                     CNEGDLEWVPYDQVLSKPTWEGDRHFQEWLLEDKPFFSACFRYDGDKLLDYSVDFYEE
                     "
     gene            2081..2644
                     /gene="folE"
                     /locus_tag="VCA20_002087"
     CDS             2081..2644
                     /gene="folE"
                     /locus_tag="VCA20_002087"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEP1835832.1"
                     /translation="MSKQEQIEQTIYQLLELLGEDPNREGLLDTPKRVAKMYLEMFNG
                     LEEDPKDQFTAVFSEGHEEVVLVKDIPFHSMCEHHLVPFYGIAHVAYIPSKGRVTGLS
                     KLARSVEVASRRPQLQERLTHQVAHALQDALEPEGVFVMVEAEHMCMSMRGIRKPGSK
                     TVTTVALGKYKEDAILRRELLSMIHNK"
     gene            2653..3465
                     /gene="folP"
                     /locus_tag="VCA20_002088"
     CDS             2653..3465
                     /gene="folP"
                     /locus_tag="VCA20_002088"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEP1835833.1"
                     /translation="MSIEQLANQVTIMGILNVTPDSFSDGGHYNEVESALVQVEKLLA
                     EGATVIDVGGESTRPGATFVSEEDEITRVVPIIRAIKENYDCLVSVDTYKTGTARAAL
                     EAGADILNDVWAGLYDGDMLALAVEYKVPIILMHNQKDESYADVVGEVKNFLAERAQA
                     ALDAGVSADQIWLDPGFGFSKNVQHNLDLLQGLEQITALGYPVLFGISRKRVVDYLLG
                     GNSLPTDRDQATAALSAWAVQKGCKMIRVHNVAANRDIVKVWDQLTSGGQHG"
     gene            3458..3820
                     /gene="folB"
                     /locus_tag="VCA20_002089"
     CDS             3458..3820
                     /gene="folB"
                     /locus_tag="VCA20_002089"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEP1835834.1"
                     /translation="MDKISLNKCRFYGYHGAFKEEQVLGQIFTVDCDLFVDLTAASQT
                     DNLEDTVHYGMVFETIKAVVEGKPYILIEKVAGVICQEIFDQFPKVEKIRLAIYKENP
                     PIAGHYDSVGIELERERP"
     gene            3817..4305
                     /gene="folK"
                     /locus_tag="VCA20_002090"
     CDS             3817..4305
                     /gene="folK"
                     /locus_tag="VCA20_002090"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEP1835835.1"
                     /translation="MKHLAYLSIGGNMGNRQAYLQSALDKLASHPGCQLGLVSNIYET
                     PAWGKTDQADFLNLACQVYTDLSAQDFLSVCQNIEQELDRVRIEKWGQRTIDLDIIFW
                     DEEKIQEENLIVPHPYAHERAFVLKPLAEIASDYCHPILKKVVSDLLAELGEQKDIKI
                     FK"
     gene            4356..5563
                     /locus_tag="VCA20_002091"
                     /pseudo
     CDS             4356..5563
                     /locus_tag="VCA20_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
BASE COUNT         1592 a         1079 c         1224 g         1668 t
ORIGIN      
        1 cgcggaacag ggacatagac tgttacccct tcctccttac tcaccagagt ataatcctat
       61 caagaaaaca tgggctcaca tcaaaaaaca cctcagaaga gtattgccga attgcgatac
      121 gtttcttgag gcacttttat cctgttcttg tttcagtcga ctataaaaag aatggtcgag
      181 aaaaaattcg gatgcgtgtc tacgattcta aaccttctga atcaagtcaa gcttttcttg
      241 aaatcaagaa aaaagaaaat aaaattggct ataaataccg tttaacctca aaccctgttt
      301 ctgtaacaaa ttatatcgaa aatggagtga ttgattccac aatcaaaaac gataaagtca
      361 catcagaact agagcaattg agagaacgct acgggataat taagcctaaa atgtatatct
      421 actacgatcg tgtatcctac aaaggaatcg aagacaaaaa agttcgtcac acaattgata
      481 agaacttact ttattgagat tacgatgtag atgccatgga gggcaaattt ggtaaggacc
      541 ttttggatcc aacaaaagtc atcatggaag taaaagttcc agaagaacga ccagactggt
      601 tggtagcact actcgaaaaa taccagattg aaaaacaatc attttcaaag tatggcaatg
      661 cctacaagct tgcccacaac atcactggag aggaagtaag tcaacatgca gttgtttaac
      721 agtatctttt caactgccaa tagcagtatc accttaacac agatggcaat ggtctttgca
      781 gtcagtctgt ctatgggggt gctcttagca gctgtttata aatataaatc caattataca
      841 aaggaatttg tcatcacact aagtttaatg ccagctttga ttgctgttat cattgcatta
      901 gttaatggta acttgggagc gggtgttgcg gtagcaagga ctttcagtct aattaaaatc
      961 tgctcagcag caggttcttc aaaggaaatg cttgcagtcc tcttagcgat ggctatcggt
     1021 ctagcgacag gaatgggctt cctaggtttg gctgtcttta tgacggtcat cctatctgct
     1081 tgtatcttga tttttgagga catcagcttt gctcaagtca atcaaaatcg tcgtcatctc
     1141 ttagtgacag tgccaaggaa ttttgactac gatcaatttt ttgaaaatca atttggtaaa
     1201 tcttgcaagc aagccgactt aatttccctc aaatacaaga agaataaaga agcgctgatc
     1261 ttagaatatc aagttctcct cgataaaaaa ttacagataa acgcttggta gatacagtat
     1321 tgtcagccgg tcctttggat gtcgtattga acaagcaaat gccgaagaag aagtatttgt
     1381 aatatcaaag aagccctgga tttccaaggc ttttttcgtt ttttattagc ttattcttca
     1441 taaaaatcaa cgctataatc aagcagtttg tccccatcat agcggaagca agcggaaaaa
     1501 aatggtttgt cctctaatag ccattcttga aaatgccggt cgccctccca ggttggtttg
     1561 gacaagactt ggtcataggg aacccactct aagtcacctt cattgcagtc aataagactt
     1621 ccttcaaaac cagttatctt aaagacatag gtgtaccaat ctgtgtttgg agtaaagtct
     1681 gggaaggtta tcaccccttt tagagcatgc tttgttacag taagcccagt ttcctcaaag
     1741 atttctcgaa tggcacaagc ctgtggcgtt tcgccaggtt ctagtttgcc accaactcca
     1801 atccattttc cttgatggac atcgttttct ttcttgtttc gatgaaggag aagaaattcc
     1861 ttaccattat caatgtagca aatcgtagcg agtaggacag gtttcttggt catagtagct
     1921 ccttgtagtt agtttgttcc tattatatca caaaggtgag agtagggaca cctgcttggt
     1981 gctctggtag gggacagttt tggtattggc atactgaaag cccttttctg aaaaatgttt
     2041 ttagggtata ataagaacag caaactattt aggaagcatt atgtcaaaac aagaacaaat
     2101 cgaacaaacc atttatcaat tattggaatt attgggtgag gacccgaatc gtgaaggttt
     2161 attagatacg cctaaacggg ttgctaagat gtatttagaa atgtttaacg gcctagaaga
     2221 ggatcccaaa gaccaattta cagctgtctt ttcagaaggt catgaagagg ttgttttggt
     2281 caaggatatt ccttttcatt ctatgtgtga acaccatttg gtaccctttt atggcattgc
     2341 ccatgtagcc tatattccga gtaaggggcg tgtgacaggt cttagcaaat tggcgcgttc
     2401 agtggaggtg gcaagtcgtc gccctcaatt gcaggagcgt ttaactcatc aagttgctca
     2461 tgcgcttcaa gatgcccttg aaccagaagg cgtttttgtc atggtcgaag cagagcatat
     2521 gtgcatgagt atgcgtggta ttcgcaagcc aggtagtaag acagtgacca cagttgccct
     2581 tggaaaatac aaggaagatg ccatcttgcg ccgtgaactc ttgtccatga ttcacaataa
     2641 ataggaggtt ctatgtcaat cgaacagtta gcgaatcaag taaccattat ggggattctc
     2701 aatgtgacgc cagattcatt ttcagatggt ggtcactata atgaggtgga gtctgccttg
     2761 gtacaagtag aaaagttgtt ggctgagggt gctaccgtca ttgatgttgg tggtgagtcc
     2821 actcgtccag gtgcaacatt tgtgtccgaa gaagacgaga ttactcgcgt ggttccgatt
     2881 atccgcgcta tcaaagaaaa ctatgactgt cttgtcagcg tcgataccta taaaacagga
     2941 actgcacgtg cagccttgga agcaggcgca gatattttaa atgatgtctg ggcaggactt
     3001 tatgatggag atatgttggc cctggctgtg gagtacaagg taccaattat tctcatgcac
     3061 aaccagaaag acgaaagcta tgcggatgtt gttggagagg tcaaaaactt tctagccgag
     3121 cgtgcccaag cagcacttga tgctggtgtt tccgcagacc agatttggtt ggatccagga
     3181 tttggctttt caaagaatgt ccaacataat ctggatctct tacaaggttt ggaacagatt
     3241 actgcccttg gttatcctgt tctttttgga atttcccgta agcgtgtggt agattatctg
     3301 ctcgggggaa atagtcttcc gactgaccgc gatcaagcaa cggcagcctt gtctgcctgg
     3361 gcagtccaaa aaggctgtaa aatgatccgt gttcacaatg tcgctgccaa ccgtgatatc
     3421 gttaaggttt gggaccaatt gacttctgga ggtcaacatg gataagattt ctctcaataa
     3481 atgccgtttt tacggctacc atggtgcctt caaagaagaa caagttcttg gtcaaatttt
     3541 cacagttgac tgtgatttgt ttgttgacct gacagcagct tcacaaactg acaatttaga
     3601 agacacagtt cactatggta tggtctttga aaccattaaa gcagttgtgg aaggcaagcc
     3661 ctacatcctt atagaaaagg tagcaggtgt tatttgtcag gagatttttg atcaatttcc
     3721 aaaggtggag aagattcgct tggccattta caaggaaaat ccacccattg ctggacacta
     3781 tgattctgtc ggaattgaat tggagcgtga acgaccatga agcatctagc ctatctcagt
     3841 atcggaggga acatgggcaa tcggcaagcc tatttacagt cagctctaga caaattagcc
     3901 agtcatccag gttgccagct tggcttagtt tccaacattt atgaaactcc agcttggggc
     3961 aagaccgacc aggctgattt cctcaatcta gcctgtcaag tgtatactga tttgtctgct
     4021 caagattttc taagcgtttg ccagaacatc gaacaagagt tggaccgagt acggattgaa
     4081 aaatggggcc aacgcaccat tgatttggat attatttttt gggacgagga gaagattcaa
     4141 gaagagaatc tgattgttcc ccatccgtac gcccatgaaa gagcatttgt tctcaaacct
     4201 cttgctgaga ttgcttctga ttactgtcac ccaattttga aaaaagtggt atcggacttg
     4261 cttgcagaac taggggaaca aaaagatata aaaattttca aatagttgcc tctgatacta
     4321 ggtttattag aggatagatt agataaagga gttttatgac aattttagca attttaggag
     4381 ttttactggc cgttgtggcc attatttatt ggacatcaaa aaatttacat gttatcattg
     4441 ctgcaccact agctagttta atcatcattc tgaccaatca gatgaatgtt ttggaagtta
     4501 tgttgggcaa agagcagtct tatatggcgg gcttagcaga ttttttgatt aataattttg
     4561 ccatctttat gttggggtct atcttggcac gttatatgga ggcaagtgga gctacgcaga
     4621 cgattgccaa tagcattttg aaagtaatgg gaaaagatag tccctataag ggcttgttag
     4681 ccataacttt gattgcttcc attatgacct atggtggtgt gagtattttt gtagctattt
     4741 ttaccttgct tccctttcac gccccgtttt taaagagttg aatatcaact gggcactttt
     4801 tccgattcct gtctttttgg gagtagggac ttatacgatg acaactcttc ctggggcacc
     4861 ttccatccag aatgttattc cgacaaaagt tttgggaact agcttgaccg cggcacccgt
     4921 tatcagtttg gcagccagct tgacgttatt agtatttggc ttgctctaca tggcctactg
     4981 cctcaagaaa agtctagcaa atggagagac gtatacagaa gaagaggatg aaactgcagt
     5041 agtggtagct gataagacac caaatctatt tttatcagtc ttgcctctat tcagtcttat
     5101 tgggacgatt ttcctaatga gtaaaactcc gaatgtccta gcaatcggat tgttggtctc
     5161 tatccttcta ttagccttga ttttttatcc atatctgcca aatcaaaaag aaattttgaa
     5221 tgctgcgaca acagcatcta tagtgcctgc ttttgcaact tctagtacag tagcatttgg
     5281 gacggtgtta accttgtcag caggttttgc agtcattcaa gaatggattc aacaaatacc
     5341 aggatcgccg ctgattagtt tatctgtttc gacagcattg gttagtggta ttatcggttc
     5401 atcttctggt gcagtaggta ttgcttcaag taatttcctt cctgcctatt tagaaatggg
     5461 gattaatcca gagttgctgc atcgtgtagt agttgttgca tcagctatct tgacagttgt
     5521 gcctcagtct ggcgtgatga ttaccttcca caatctttcg aaa
//
LOCUS       DATUCG010000138         5416 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.140, whole genome shotgun sequence.
ACCESSION   DATUCG010000138 DATUCG010000000
VERSION     DATUCG010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5416
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.140"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(200..2185)
                     /gene="uvrB"
                     /locus_tag="VCA20_002092"
     CDS             complement(200..2185)
                     /gene="uvrB"
                     /locus_tag="VCA20_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEP1835836.1"
                     /translation="MINRNTENQFKLVSKYAPSGDQPQAIETLVDNIEGGEKAQILMG
                     ATGTGKTYTMSQVIARVNKPTLVIAHNKTLAGQLYSEFKEFFPENAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSALLERNDVIVVASVSCIYGLGSPKEYS
                     DSVVSLRPGQEISRDQLLNALVDIQFERNDIDFQRGRFRVRGDVVEIFPASRDEHAFR
                     VEFFGDEIDRIREIESLTGKVLGDVDHLAIFPATHFVTNDDHMETAIAKIQAELEEQL
                     KVFEAEGKLLEAQRLKQRTDYDIEMLREMGYTNGVENYSRHMDGRSEGEPPYTLLDFF
                     PEDYLIMIDESHMTMGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYEMEQTETVVEQIIRPTGLLDPEVEVRPTMGQMDDLLGEINARVEKG
                     ERTFITTLTKKMAEDLTDYLKEMGVKVKYMHSDIKTLERTEIIRDLRLGVFDVLIGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSEGHVIMYADKVTESMR
                     KAMDETARRRQIQMAYNEEHGIIPQTIKKEIRDLISVTKTVNQDKEEVVDFNALNKDE
                     RKAMIKKLEGQMQEAAEVLDFELAAQIRDMVIELKNM"
     gene            2383..4581
                     /locus_tag="VCA20_002093"
     CDS             2383..4581
                     /locus_tag="VCA20_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEP1835837.1"
                     /translation="MKKKLLMLLASILPVFFIFTGVKADDTIDIVFDNAYAPFEFKDS
                     DQIYKGLDVDIINEVAKRSGWTMNQSFPGFDAAVNAVQAGSADALMAGTTITEARKKV
                     FTFSDPYFDTKIVIATTKANTISSYKDLKGKTVGVKNGTAAQNFLKENKDKYGYNVKT
                     FDTGDLMYNSLSAGAVDAVMDDEAVIQYAIQQGQDLSINIKGEAIGSFGFSVKKGSQY
                     EYLVEDFNKALAEMKKDGTYETIMNKWLGASTTSAESTDYSSRLSLTGNASAKATPVK
                     SSYTIVADSSFAPFEYQDESGNYVGIDMELIKAIAEQQGFTITIQNPGFDAALNAVQA
                     GQADAVIAGMSITDARKEIFDFSNAYYTSNILLAVKNGSDIASYEDLKGKTVGAKNGT
                     ASYSFLEENKAKYDYTLKAFDEASSMYDSLNSGSIDALMDDEAVLLYAIQQGRNFATP
                     IPGEKSGEYGFAVKKGANPELIEMFNNGLAALVESGKYDEILNKYFNSTEEASTTTST
                     VDETTIVGLLKNNYGQLLSGLGITIGLALLSFAIAIVIGIIFGMFAVSPVKALRVTSS
                     VFVDVVRGIPLMIVAAFIFWGIPNLIESITGQQSPINDFVAGTIALSLNSGAYIAEIV
                     RGGIQAVPAGQMEASRSLGISYGTTMRKIILPQAGKIMLPNFINQFVITLKDTTIISA
                     IGLVELFQAGKIIIARNYQSFRMYAILAIIYLVVITLLTRLARKLEKGGK"
     gene            4581..5321
                     /locus_tag="VCA20_002094"
     CDS             4581..5321
                     /locus_tag="VCA20_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEP1835838.1"
                     /translation="MAQLKINVHDLHKSYGKNEVLKGITAKFYEGDVVCIIGPSGSGK
                     STFLRTMNLLETITSGQVTVDGYDLTDPKTDVDAFRSNVGMVFQHFNLFPHMSVLDNI
                     TFAPIEHKLMDKSEAEKVGMELLEKVGLADKRDAMPDSLSGGQKQRVAIARALAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKDLAEQGMTMLIVTHEMGFARKVANRVIFTDGGEF
                     LEDGTPEQIFDNPQHPRLQDFLDKVLNV"
BASE COUNT         1528 a         1249 c         1089 g         1550 t
ORIGIN      
        1 aaatattttt cataaaaact ccccctattc cctaaatagt atgaaaacca ttataacctt
       61 ttatagggac agggacaaat tttttgtccc taagtcccca agtaaaaatt ttaagtatta
      121 taaaaggtgg caggtcaatt tctacctgcc gcctcttgta atttcattct acttaaatca
      181 ctgaacctag caactcttac tacatattct tcaactcaat aaccatatca cgaatctggg
      241 ctgccagttc aaagtctagc acttctgctg cttcctgcat ttgaccttcc agtttcttga
      301 tcatagcctt gcgttcgtct ttgttgaggg cattgaagtc caccacttct tccttgtcct
      361 gattgacagt cttggtcacg ctaatcaggt cacggatttc ctttttaata gtctgtggaa
      421 tgataccatg ttcttcatta taagccattt ggatttgacg gcggcgggct gtttcgtcca
      481 tagccttgcg catggactca gtgaccttgt ccgcatacat aatcacatga ccctcagagt
      541 tacgagctgc ccgaccaatt gtctggatga gcccccgttc attacggagg aaaccttcct
      601 tgtcagcatc tagaatagca accagactga cttctggcac gtcaatccct tcacgcaaga
      661 ggttaatccc tatcaagaca tcaaagacgc ccaaacgcaa atcacggata atctccgtac
      721 gctccaaggt cttgatgtcc gagtgcatat acttgacctt gacacccatt tctttcaggt
      781 agtcggtcaa gtcctctgcc attttcttgg tcagggtagt gataaaggtt cgctcgcctt
      841 tttcaacacg tgcattgatt tcgcccaaaa ggtcgtccat ttggcccatg gttggacgga
      901 cctcaacttc agggtccaaa agccctgtcg gccgaataat ctgctcgaca acggtctctg
      961 tctgctccat ttcataatca cccggcgtcg cagatacata gacaatctgg tggacatggc
     1021 tctcaaattc ttccctgcgc agcggacggt tgtccaatgc actcgggagg cggaaaccat
     1081 agttgaccaa catctccttg cgtgaacggt caccattgta catgccctta atctgcccca
     1141 tggtcatgtg gctctcgtca atcatgatga gataatcttc agggaaaaag tccagcaggg
     1201 tataaggagg ctcgccctcg cttcgcccgt ccatgtggcg agaatagttc tcgactccgt
     1261 tggtgtagcc catttcccga agcatctcga tgtcgtagtc tgttcgttgt ttcaatcgct
     1321 gagcttctaa gagttttcct tctgcctcaa agaccttgag ctgctcttcc agttccgcct
     1381 gaatcttggc aatagccgtt tccatatggt catcgttggt cacgaagtgg gtggcaggga
     1441 aaatcgccaa gtgatccaca tcgcccaaaa ccttcccagt caggctttca atctcacgaa
     1501 tgcggtcgat ttcatcgccg aaaaactcca cacgaaaggc gtgttcatct cgagaagccg
     1561 ggaaaatctc caccacatct ccacgtacac ggaaacgccc ccgttggaag tcgatgtcgt
     1621 tgcgctcaaa ctggatatcc accaaggcat tgagcaactg atcacgggaa atctcctgac
     1681 ctggtcgtag gctgaccacg ctatctgaat attccttagg tgaacccaaa ccgtagatac
     1741 aagaaacaga agccaccaca atgacatcat tgcgttctag cagagctgaa gtcgcagagt
     1801 gacggagttt gtcaatttca tcattgaccg aactgtcctt ctcaatatag gtatcgctag
     1861 acggtacata agcttctggc tggtagtaat cgtagtaaga cacaaagtat tcgaccgcat
     1921 tttctgggaa gaactcctta aactcactat aaagctgacc agccaaggtc ttattgtggg
     1981 cgatgactag ggtcggctta ttgacacggg cgatgacctg actcatagtg taggtcttac
     2041 cagtacccgt cgcccccatg agaatctggg ctttttcgcc cccctcgatg ttatcaacca
     2101 aggtttcgat agcttgaggc tggtccccag acggtgcata tttggaaaca agtttaaatt
     2161 ggttttcagt atttcgattg atcatactcc tattttatca taagttcgac taatggagaa
     2221 acattctaaa ccattcacaa ctatgtccaa aatattaaat tttctcacat tttaatgact
     2281 ttgtatgtca gaaattctaa cattataaaa acctcgattt tgcattaatt ttcagaatat
     2341 gatatactag gacagttatt ttataataga aggagtatgg aaatgaagaa aaaattactc
     2401 atgttattgg caagcattct gccagtattc tttattttta ctggcgttaa agctgacgat
     2461 actattgata tcgtatttga caatgcttac gccccattcg agtttaaaga ctcagatcaa
     2521 atttataaag gattagatgt tgacatcatc aacgaagtag ccaagcgctc aggttggacc
     2581 atgaatcaaa gcttcccagg atttgatgca gctgtaaacg ctgtacaggc tggatctgcg
     2641 gacgccctaa tggcaggtac aactatcacc gaagcgcgta agaaagtatt tactttctca
     2701 gacccttact tcgacaccaa aatcgttatc gctaccacaa aagcaaatac catctcttca
     2761 tataaggacc ttaaaggcaa gactgtcggt gtcaaaaacg gtaccgcagc ccaaaacttc
     2821 ctaaaagaaa acaaagacaa gtacggatac aacgtaaaaa cttttgatac tggcgatttg
     2881 atgtataata gtctttctgc tggtgctgtt gatgcagtca tggacgacga agcggttatt
     2941 caatacgcta tccaacaagg tcaagatcta agcattaaca tcaagggcga agccatcggt
     3001 tcatttggtt tctctgtaaa aaaaggtagc caatatgaat atcttgttga agactttaac
     3061 aaggctttag ctgagatgaa gaaagatggc acctatgaaa caatcatgaa caaatggttg
     3121 ggagcttcta ccacttctgc cgaaagtaca gattactcat ctcgtttaag cttaacagga
     3181 aacgcatcag ctaaagcaac acctgtcaaa tcaagctata caattgtggc agattcttct
     3241 tttgctccat ttgaatacca agatgaatca ggtaactatg tcgggattga catggaatta
     3301 atcaaggcaa tcgctgaaca acaaggattt actattacca ttcaaaaccc tggtttcgac
     3361 gctgcactaa atgctgttca agctggacaa gctgatgcag ttattgctgg tatgtctatc
     3421 acagatgctc gcaaggaaat ctttgacttc tcaaacgcct actatacttc aaacatccta
     3481 ttagctgtta aaaatggtag tgacatcgct tcttacgaag acttaaaagg gaaaactgtt
     3541 ggagctaaaa atggtactgc ttcatatagt ttcttagaag aaaacaaggc aaaatatgat
     3601 tacaccctaa aagccttcga cgaagcttca agtatgtatg acagtttaaa ctctggttct
     3661 attgatgctc ttatggatga cgaagcggtt cttctatatg ccatccaaca aggtcgcaac
     3721 tttgcaacac caattcctgg cgaaaaatca ggtgagtacg gatttgccgt taaaaaaggt
     3781 gccaacccag aattaattga aatgtttaac aacggcttag ctgccctagt tgaatcaggc
     3841 aagtatgatg aaatccttaa taagtatttc aactcaactg aagaagcaag cacaacaact
     3901 tcaactgttg atgagacaac gattgttggt cttttgaaaa acaactacgg tcaattgctc
     3961 tcaggtcttg gtatcacaat tggacttgcc cttctatcat ttgctattgc aattgtgatt
     4021 ggtattatct tcggtatgtt tgctgtcagc ccagttaaag cactccgtgt tacttcatct
     4081 gtctttgtag atgttgttcg tggtattccg ctcatgattg tcgctgcatt catcttctgg
     4141 ggtattccaa acttaatcga gtcgattact ggccaacagt caccgatcaa tgactttgtc
     4201 gctggtacca ttgccctatc cctcaactct ggtgcctata ttgctgagat tgttcgtggc
     4261 ggtattcagg cagttccagc tggtcagatg gaagcatctc gcagtttggg tatttcctac
     4321 ggaacaacca tgcgtaagat tatcctacct caagctggta aaattatgtt gcctaacttt
     4381 atcaaccagt ttgttattac tctgaaagat actactatca tttctgcgat tggtttggtt
     4441 gaactcttcc aagcaggaaa aatcattatt gcacgtaact accaatcctt ccgtatgtat
     4501 gcaattcttg ccatcattta cttggtagtt atcacactct tgactcgctt agcacgcaaa
     4561 ctcgaaaaag gaggcaaata atggctcaat taaaaatcaa cgtccatgac ttacacaagt
     4621 cttatggtaa aaacgaggtc ctcaaaggta ttactgctaa gttctatgaa ggcgatgttg
     4681 tttgtatcat cggtccttct ggttcaggta aatcgacctt ccttcgtaca atgaacttac
     4741 ttgagaccat tacaagtggt caagtaacag tcgatggata tgacttaacc gatcctaaga
     4801 cagatgttga cgccttccgt tcaaatgtcg gaatggtatt ccaacacttc aacctcttcc
     4861 ctcatatgtc tgtacttgac aatatcacat ttgcgccaat cgaacacaaa ttgatggaca
     4921 agtctgaagc tgaaaaggtc ggcatggaat tattggaaaa agttggattg gcagacaaac
     4981 gcgacgctat gccagacagc ctttcaggtg ggcagaagca acgtgttgcc atcgctcgtg
     5041 ccctcgctat gaacccagac atcatgctct tcgacgaacc aacttctgcc cttgaccctg
     5101 aaatggttgg cgatgtactc aacgttatga aagacttggc tgagcaaggt atgaccatgt
     5161 taatcgtcac acacgagatg ggctttgccc gcaaggttgc taatcgcgtt atcttcactg
     5221 atggcggtga gttccttgaa gatggtacac cagagcaaat ctttgataac ccacaacatc
     5281 cacgtttaca agacttcctt gataaggtct tgaacgtcta agctaaacga aaagccttga
     5341 gttgcatcaa ggtttcagat tgaagaaaaa gtccattttg gacgattttc ttcaatcttt
     5401 tttctttatg ttgaaa
//
LOCUS       DATUCG010000139         5376 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.141, whole genome shotgun sequence.
ACCESSION   DATUCG010000139 DATUCG010000000
VERSION     DATUCG010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5376
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.141"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(93..944)
                     /locus_tag="VCA20_002095"
     CDS             complement(93..944)
                     /locus_tag="VCA20_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP1835839.1"
                     /translation="MTEKFRLKRKELGLSQQTLAKGICEQSQISKIERGHFIPSADLL
                     FRLSQRLEVPLDFFNEQIEVKSNLSNFKHLSARLLDDRNYDDLEYLYKIEVERSTFLT
                     LEDRTYLEWIKAIIDFYQYELQFEAISHLENILSKVASTTLIYLKVLNTLSNFYSLIG
                     REEDYEANYSHLIELYQTKNLDHQEVLFGYIRVRYNYAHYLVSKEKYNEAMQEALETI
                     ELCKERQTSYQLAPLLVLVGNCGTQFLGKEQVKNYYIEARELCKIYNNPLMLMKIENY
                     LKELDTF"
     gene            1133..1303
                     /locus_tag="VCA20_002096"
     CDS             1133..1303
                     /locus_tag="VCA20_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835840.1"
                     /translation="MKKSRGIQKLAILALLGVFTFSNTIPYQQFIQKNKRLEIRVDSQ
                     KKSDGLDVGKAD"
     gene            1619..1915
                     /locus_tag="VCA20_002097"
     CDS             1619..1915
                     /locus_tag="VCA20_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000911560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEP1835841.1"
                     /translation="MLKIRKVVASSLAVLAFSLALPVLAVSHLGGEWTYGGHHDPNNW
                     GAFSNYYHGSQYHWAYVGSNGRNNQKTAYAGAHSAVYAFVNTNFGEQVTFDAGW"
     gene            2034..4034
                     /locus_tag="VCA20_002098"
     CDS             2034..4034
                     /locus_tag="VCA20_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEP1835842.1"
                     /translation="MLISNLLASLFFVWVSTIWTDTYVSYYYPNVVVRDSSPETTFQH
                     VATRLEKLAEETDSFIAIQHQDPNSEGTTVFSYTTFGVGKLPDGLQERKLEDAQSSSV
                     ETNYFVFDGHLDIHLLREELSQLGLTNMHLTIPSKLSTLMAIFSNGFQLISLLIFILT
                     FGALTLISQISQLRSSGIRLISGEKRWSIFLRPVGEDLKGIAVGFSLAGVFAILMQKI
                     LSLPTESLMTIGAGLLSYNLILLLISLFFAQLFAVGIKKVHLMQIIKGQVPVRGIISL
                     ILIGQLLAIIIVTLGIGSSLKYSQAWQQHRIGQEVWSQERQLIILSISREGTSPGFDE
                     QAQRQLRTWYQLMDLAVSEQKAFLSRHQLIDRTLQNGMASSKNLTTSTEWHDYSPNGN
                     ILIVTPQYLERQNIPVDTTIEQKMNHLDVGEFVLLLPEHLRSEEEHYKSVFEDDLTSR
                     MSSQDERQQMTATVGYLESGQDRFVYNTTPISYQQFLKDPIIIVITPQSTGPQSIVFW
                     VDAVQNYVLFNQLSDAQELIQRQGIENWVSEMPSGYNNYITLSNNIQRERWVMLAGAV
                     LGIATSILLFNTMNRLYFEEFRRAIFIKRIAGLRFLEIHRTYLLAQLGVFLLGFVASV
                     FLQVEVVVAFLVLLLFIGVSLLQLHVQMQKENKMSMLVLKGG"
     gene            4036..4668
                     /locus_tag="VCA20_002099"
     CDS             4036..4668
                     /locus_tag="VCA20_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1835843.1"
                     /translation="MIELKRVSKSFGERELFSNLSMAFEAGKVYALIGSSGSGKTTLM
                     NMMGKLEPYDGTIFYRGKDLASYKSSDFFRHELGYLFQNFGLIENQTIEENLKLGLVG
                     QKLSRSEQRLKQKQALEQVGLAYLDLDKRIFELSGGESQRVALAKVILKNPPFILADE
                     PTASIDPATSQLIMEILLSLRDDNRLIIIATHNPAIWEMADEVVAMDSLK"
     gene            4981..5337
                     /locus_tag="VCA20_002100"
                     /pseudo
     CDS             4981..5337
                     /locus_tag="VCA20_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636572.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
BASE COUNT         1643 a          917 c         1107 g         1709 t
ORIGIN      
        1 tcgtgatgtg ggcttatagc ccttgttcaa accacccgtt tgacgggtgg tcatgattta
       61 acatttaagt agaactccaa tatcaagata aattaaaaag tatctaattc cttcaaataa
      121 ttctctatct tcatcagcat taaaggattg ttataaatct tgcatagctc tcttgcttct
      181 atataataat ttttgacttg ttctttgcca aggaattgtg ttccacaatt tccaacgaga
      241 acgagcaggg gagctagttg gtaacttgtt tgcctctctt tacagagttc gatagtctca
      301 agggcttctt gcatggcttc gttgtatttt tcttttgaga ctaaatagtg agcatagttg
      361 taacgaactc tgatataacc aaacaaaacc tcttgatggt ccaaattttt tgtctgatac
      421 aactctatta aatgagagta gtttgcttca taatcttctt cacgaccaat aagggaatag
      481 aagttagaca gtgtgtttaa tacttttaag taaatcagag tagttgaggc tacttttgat
      541 aatatatttt ccaagtgaga gatagcctca aattgaagtt catattgata gaagtcaata
      601 atagctttaa tccattcaag gtaagttcga tcttctagtg ttagaaaggt gcttcgctca
      661 acctctattt tataaagata ttctaaatcg tcataatttc tatcatccaa tagacgagca
      721 gataaatgct tgaaattaga gaggttagac ttaacttcaa tttgttcatt aaaaaagtct
      781 aatgggactt caagtcgttg agagagtctg aatagaaggt ctgcggaggg aataaaatgt
      841 cccctctcaa ttttactaat ctggctctgt tcacaaattc cttttgcaag agtttgttgg
      901 gagagtccca actcctttct ttttaatctg aatttttcag tcataaaaac aaaaaacctt
      961 cctaatttat ctttgtcaat tatatatcat ttagttaaag acggcaaata ttttctcgat
     1021 aaaattacaa ataggaataa taataaaaaa agccaaaaat tgcataaaaa tatttgacta
     1081 aataaaatat atctgttaaa ataaagacaa ggaaacagaa ggggtttcac aaatgaagaa
     1141 aagtcgtgga attcaaaaat tagctatatt agcattacta ggggttttta cgtttagtaa
     1201 tacaattcct taccaacagt ttattcagaa gaataaacga ttggagattc gagtagactc
     1261 acaaaagaag tctgatggtc ttgatgttgg gaaggctgat tgatttcgta ttactttaaa
     1321 attcaactat ttagagaaat gtattctgat gagagcttgt aacaatacca gatagatata
     1381 agaatggaga tgtttgataa aatgctttca tagctaacca actgttgaaa attatgagtt
     1441 acgaaatcta aagcatttaa gattggttta taaaattagg tctctgtctg tagaaaagaa
     1501 cgtgcagtta gaaaggagga aaaaatagga tactataatt cagactgaga tttctaatta
     1561 aaatatttag ttgcacttcg ctcattttca taatattccg ttttagaggc gtaatactat
     1621 gttaaaaatt agaaaagtag ttgcatcatc ccttgccgtt ttggcattta gtttggctct
     1681 tcctgttttg gcagtttctc accttggtgg cgagtggacc tatggaggcc atcatgatcc
     1741 aaataattgg ggagcatttt cgaattatta tcatggttca cagtatcact gggcctatgt
     1801 tggaagtaat ggacgtaaca atcaaaaaac agcttatgca ggagcacata gtgcggtcta
     1861 tgcgtttgtt aatacaaatt ttggggagca agttacattt gatgcgggat ggtaaagaat
     1921 aattgaagaa gattctttta tcaatagttt agtaagcttg ttagtttata gagggcagtg
     1981 atatttctgc cctctatttt agaataagga atgcactatg aaaaaaacta tttatactca
     2041 tctccaacct gcttgctagt ttgttctttg tttgggtttc taccatttgg acggacacat
     2101 atgtatccta ttattacccc aacgttgttg tacgtgattc ttcccctgaa acaacttttc
     2161 agcatgttgc tacacgcttg gaaaaactag cagaagaaac ggatagcttt attgcgattc
     2221 aacaccaaga tcctaactca gaaggtacta cagttttttc ttatacgacc ttcggggtcg
     2281 gaaagttgcc tgatgggctt caggaaagaa agctagaaga tgctcaaagt agtagtgttg
     2341 aaacaaacta ttttgtattc gatggacacc tagatattca cttgctaagg gaggagctaa
     2401 gtcaacttgg cttgactaac atgcacctga caattccatc taaactatct acgttaatgg
     2461 ctatctttag taacggattt cagttaatca gtctattgat tttcatctta acctttggag
     2521 cattgacttt aattagtcaa attagccaat tacggtcgtc gggcattcgc ctcatttcag
     2581 gagagaaacg gtggtccatt ttccttagac cagttggtga ggatttgaaa gggatagctg
     2641 ttggtttcag tttagctggc gtgttcgcca ttcttatgca aaaaattctg tcgcttccaa
     2701 cggaatccct gatgaccata ggagcaggat tgctcagtta taatctcatc ttgttgttga
     2761 tctccctgtt tttcgctcaa ctcttcgcag ttgggataaa aaaggtacac ctgatgcaga
     2821 ttataaaagg gcaagtgcct gtcagaggaa tcattagttt gattttaatc ggtcaactat
     2881 tagcgattat tattgtaacg ctgggaatag ggagtagctt aaagtattcc caagcctggc
     2941 aacagcatcg gattggacaa gaggtttgga gtcaggaaag gcaactgatt atcctatcaa
     3001 tcagccgtga aggaacgagt cctggttttg atgaacaagc tcaaaggcaa ctcagaactt
     3061 ggtatcaatt gatggatctg gctgtttcag aacaaaaggc tttcttatct agacaccagt
     3121 taattgaccg tactttgcaa aatggcatgg cttcctccaa aaacttgaca acctctacag
     3181 aatggcacga ttacagcccg aatgggaata ttcttatcgt cacaccgcaa tacttggagc
     3241 gtcaaaacat tcctgtagat acaactattg agcaaaagat gaatcacctt gatgtagggg
     3301 agtttgtctt attgctgcct gaacacctcc gttcagagga agaacattat aaatctgttt
     3361 ttgaagacga cttaaccagt cgcatgtcta gtcaagatga acgacagcaa atgactgcta
     3421 cggtaggtta tttggaatca ggtcaggatc gttttgtcta taatacgacc cctatttctt
     3481 accagcagtt tttgaaagat ccaatcatca ttgttataac accccaatca actggtccac
     3541 agtccattgt gttttgggta gacgcagtac agaactatgt tctctttaat caattgtctg
     3601 atgcccagga gcttatccag agacaaggca ttgaaaattg ggtctcagaa atgccatcgg
     3661 gatataacaa ctacatcaca ttatcgaata atatccagag ggaacgttgg gtaatgttgg
     3721 ctggagcggt gcttgggatt gcaacttcga ttttgctgtt taacaccatg aataggctct
     3781 actttgaaga atttagacgt gccatcttta tcaaacggat tgcaggtctt agattcttag
     3841 aaatccatcg gacttacctc ttggctcaac tgggtgtgtt tttactggga tttgtggcga
     3901 gtgtattcct tcaggtagag gtagtggtcg cttttttagt cttattgctc tttattggtg
     3961 tatctctttt acaactacat gtccaaatgc agaaagaaaa caagatgtcc atgcttgttt
     4021 tgaagggagg ttaatatgat tgaacttaaa cgggtaagta agtcttttgg agaaagagag
     4081 ttgttttcga atctttcaat ggcatttgag gctgggaaag tctatgcctt gatcggttca
     4141 agcggtagtg ggaaaacaac cttaatgaac atgatgggga aattagaacc ttatgatggg
     4201 acgatttttt accgtggtaa agacttggcc agttataagt cgagtgattt tttccgtcac
     4261 gaattgggtt acctcttcca gaactttggc ttgattgaaa atcaaaccat agaagaaaac
     4321 cttaaactag gtctcgttgg tcaaaagttg agtcggtcgg agcaacgatt gaaacagaag
     4381 caggccttag aacaggtcgg cctggcttat cttgacctag ataagcgcat ctttgagtta
     4441 tcgggcggag aatcgcaacg ggttgccttg gcaaaagtta tcttaaagaa tccacccttt
     4501 attctggcag atgagccaac agcttcaata gacccagcaa cctctcagtt gattatggag
     4561 attttgctat ctcttcgaga tgataatagg ctaatcatta tcgcaacaca taatccggca
     4621 atctgggaaa tggctgatga agtggttgct atggatagcc tgaaataaaa atcccctgtt
     4681 tcaatagcat tttaagttac cgaaatatta tcttgacttg atacttgaag ttaatttagg
     4741 attgaagttt attaattcta ggggaacttc ggtcaaataa gtgaaagcca gcgtctttag
     4801 gcgctggctg gtgatgtggg cttatagccc ttgttcaaac cacccgtttg acgggtggtc
     4861 atgattgtct gtgaatgata caactgaaaa caaatgaaag ttcaggtttt atggtaaaat
     4921 tcttgttttt cttcaaaaat atgatagtta acaatatacc agacggaggt tgtgttaata
     4981 gtgttgactg taaataaatt ggtcaaaaca gagttgcatt gtcatttggc tggctcattg
     5041 tccctcggag tcattcgaca attggcacag atggctaaaa tcactattcc ggcagaagat
     5101 gaagcattaa gaaaattagt tagtgttcat ggaaaagttg atagtttaat ggcttattta
     5161 aagttatttg attttgttcg cccagtctta caaactgcaa cagctttaga attggcagcc
     5221 tatgatcttg tacgacaagc tgcaagtgat aaggtcattt acattgaagt tcgttttgca
     5281 cctgaactgt cgacggatca agacttgact attttagaag cggtctcagc agttttatag
     5341 ttaaatgaaa caaaaacagt acatttatga tataat
//
LOCUS       DATUCG010000140         5330 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.142, whole genome shotgun sequence.
ACCESSION   DATUCG010000140 DATUCG010000000
VERSION     DATUCG010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5330
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.142"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(203..1642)
                     /gene="gatB"
                     /locus_tag="VCA20_002101"
     CDS             complement(203..1642)
                     /gene="gatB"
                     /locus_tag="VCA20_002101"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEP1835844.1"
                     /translation="MNFETIIGLEVHVELNTNSKIFSPSSAHFGEDPNANTNVIDWSF
                     PGVLPVLNKGVVDAGIKAALALNMDIHKDMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEIELEDGATKKIRIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEIIQYTGISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNS
                     FNYVRKGLQYEVERQAKILRSGGQIQQETRRYDESTGETILMRVKEGSADYRYFPEPD
                     LPLYEIDDSWIEEVRAELPVFPKARRAHYVENLGLTAYDAGQLTSTKALSDFFEVAVA
                     AGGDAKQVSNWLQGEVAQFLNAEGKTIEQIALTPENLVEMIALIADGTISSKIAKKVF
                     VHLAKEGGSAKAYVEKAGLVQISDPAVLIPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVAQQLLAQELAKLLD"
     gene            complement(1642..3108)
                     /gene="gatA"
                     /locus_tag="VCA20_002102"
     CDS             complement(1642..3108)
                     /gene="gatA"
                     /locus_tag="VCA20_002102"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEP1835845.1"
                     /translation="MTFNNKTIDELHDLLVKKGISAVELTKATLEDIKSREGAVDAFL
                     TITEDAALAQAAALDEKGIDADNVMAGIPLAVKDNISTKGILTTAASKMLYNYEPIFD
                     ATSVSQAYAKDMIVVGKTNMDEFAMGGSNENSAFKPTKNAWDQTKVPGGSSGGSAAAV
                     AAGQVRLSLGSDTGGSIRQPAAFNGIVGMKPTYGTVSRFGLIAFGSSLDQIGPFSQTV
                     KENAQLLNVISGHDVKDATSTINEIADFTSKIGQDIKGMKIALPKEYMGEGIDPQVKE
                     TILKAAKHLESLGAIIEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGFRAEDA
                     TNLEEIYVKTRSQGFGEEVKRRIMLGTFSLSSGYYDAYFKKAGQVRTLIIQDFEKVFA
                     DYDLILGPTAPTVAFGLDTLNHDPVAMYLADLLTIPVNLAGLPGLSIPAGFVEGLPVG
                     LQLIGPKYSEETIYQVAAAFEATTDYHKQQPVIFGGAN"
     gene            complement(3108..3410)
                     /gene="gatC"
                     /locus_tag="VCA20_002103"
     CDS             complement(3108..3410)
                     /gene="gatC"
                     /locus_tag="VCA20_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEP1835846.1"
                     /translation="MKISEAEVRHVAKLSKLEFSDQETAEFATSLSKIVDMVELLNEV
                     DTTGVAVTTTMADRKNVLRADIAQKGESREELFKNVPESQDNFIKVPAILDGGGDA"
     gene            complement(3627..4301)
                     /locus_tag="VCA20_002104"
     CDS             complement(3627..4301)
                     /locus_tag="VCA20_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEP1835847.1"
                     /translation="MSEQFIPSVLSDLRQDIVQMPEVIKECSGIRIYGRRIRSVLFTT
                     DVSIIANHNADAILAVYPFTPSPAIIKSIMLVASVPVLAGVGGGLTTGMRSANMSLLS
                     ESEGAYAVVVNGPTDVKTIEAINKVVDIPIIYTVVSEKSDLTSRIKAGVDILNVSCGM
                     ETPRVVKKIREAFPDFPIIATGGPTEDSIRRVIEAGANAVSYTAPSNGELFKGKMEKY
                     RKHAKD"
     gene            4538..>5330
                     /locus_tag="VCA20_002105"
     CDS             4538..>5330
                     /locus_tag="VCA20_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEP1835848.1"
                     /translation="MEQLYHTTELIGIKDKNIKILFILKHQTHLEIRAKLDYDSPGCP
                     HCQRKCIKYDFQKSSKIPILDCQGFPTLLLLKKRRFQCKSCQKVTVAETALVKKNCQI
                     SLPIWEKVTQLHTENMTNIAIARRLHISVSVLQKKLAQFQLEHNFTKLPKVLSWDEFS
                     RNKGKLAFIAQDFETRRIVTILENNRQTTIKNYFYKYPRAVRETVEVVTVDMSGSYIP
                     IIKKLFPRAKIVLDRFHIIQHLSRAMMTTRIDIMKTFDTRSLPYRS"
BASE COUNT         1580 a         1286 c          996 g         1468 t
ORIGIN      
        1 tcatcaaatc aagtcaacaa cgtctgattt tgattttcga agagtattag ctaactatcc
       61 tttaacagtc aatacccgcg ctaccttcta atactttata ccgtttttaa aatatagcat
      121 aaaaataaga tagaagtacc tagtttacaa gtaatagtaa aaaccgctaa gattcaatcc
      181 tagcggtttt cttctattca ttttaatcca acaacttagc caattcctgt gccaaaagtt
      241 gttgtgcaac ttgtgggtta gcttgtccct tagtcgcttt catcaagaaa cctgtaaagg
      301 ccttatcagc attgcgtttg ccagacttga agtcagctac agccgcttca ttatccgcaa
      361 agacttggtg gataatcgga atgaggacag ctgggtctga aatctgtacc aagccagctt
      421 tctcaacgta agccttagca gagccacctt ctttcgccaa gtgaacaaag actttcttgg
      481 caatcttaga tgaaatagtg ccatccgcaa tcaaggctat catctcaacc aagttttcag
      541 gtgttaaggc gatttgctca atagtcttgc cttcagcatt gaggaattga gccacttcac
      601 cctgcaacca gttagacact tgtttggcat cgccacctgc tgccactgct acttcaaaga
      661 agtcagacaa ggccttggta gacgtcaact gaccagcatc ataggcagtc aagcccaagt
      721 tctccacata gtgagcacgg cgagcctttg gaaagactgg caattctgca cgaacttcct
      781 caatccagct atcatcaatc tcatagagtg gcaggtctgg ctctgggaaa taacggtagt
      841 ccgctgaacc ttctttgaca cgcatgagaa tagtttcccc agtagattca tcgtaacggc
      901 gagtttcctg ctggatttga ccacctgaac gcaagatttt cgcctgacgt tcaacttcgt
      961 actgcaagcc cttgcgcaca tagttgaagg agttgaggtt tttcaactca gtcttggtac
     1021 caaatttttc ttgaccatag ggacgaagag agatattggc atccacgcgc atagacccct
     1081 cttccatctt cacatctgaa ataccagtgt actggataat ttccttgaga gctgtcaagt
     1141 aggcataggc ctcttcaggc gagcgcatat ctgcttctga tacaatctca atcaaaggca
     1201 caccctgacg gttgaggtcc acataagaat agccatctgt cccgtgggta ttcttaccag
     1261 catcctcttc caaatgggca cgctcgatac ggattttctt ggttgctccg tcttctagct
     1321 caatctcaat ccaaccattg tagccaatcg gctcatcaaa ctgggaaatc tgataggctt
     1381 tagggttatc aggatagaaa tagttcttac ggtcaaagtg catatccttg tgaatatcca
     1441 tgttcaaggc caaggcagcc ttgattcctg cgtccacaac gcccttgtta agaacaggca
     1501 gaactccagg gaaagaccag tcaatcacgt tggtattagc atttggatcc tcaccaaaat
     1561 gagcagatga aggtgagaaa attttcgagt tggtattcaa ctccacatgg acttctagac
     1621 caataatcgt ttcaaagttc attagttagc acctccaaaa atcactggtt gttgcttatg
     1681 gtagtctgtc gtcgcctcaa aagcagcagc cacttggtaa atggtctctt ctgaatattt
     1741 tggaccaatc aactgcaaac caactggcaa gccttctaca aaaccagcag gaatagaaag
     1801 accaggaaga cctgccaagt ttacaggaat tgttaacaag tccgccaagt acatagccac
     1861 agggtcatgg ttgagcgtat ccaaaccaaa ggcaacggtc ggagcagttg gccccaaaat
     1921 caagtcatag tccgcaaaga ccttttcaaa atcttggata atcaaggtcc gcacctgacc
     1981 agccttcttg aagtaggcat cgtagtaacc tgatgataag ctaaatgtcc ctaacataat
     2041 acggcgtttg acttcctcac caaagccttg gctacgggtt ttcacgtaaa tctcttccaa
     2101 gttggtcgca tcttctgcac ggaaaccgta acggattccg tcaaaacgct gcaagttaga
     2161 agatgcctct gatgaagcaa ttatgtagta aacagcaacc ccatacttag aatgtggcag
     2221 gctaacttct tcaatgattg ctcccaagct ttccaagtgc ttagccgcct tgagaatagt
     2281 ttccttgacc tgcggatcaa tcccttcgcc catgtattct ttcggcaaag caattttcat
     2341 gcctttaatg tcttgaccaa tcttgctagt gaagtcagca atttcattga tcgttgatgt
     2401 tgcatccttg acatcatgac cagagatgac attgagcaac tgggcatttt ccttaaccgt
     2461 ctgtgaaaat ggaccaatct ggtcaagaga tgaaccaaag gcaatcagac caaaacgtga
     2521 caccgttcca taggtcggtt tcataccaac gataccatta aaggctgctg gttgacgaat
     2581 ggaaccacct gtgtcagacc ccagtgacaa acggacctga ccagcagcaa ctgcagctgc
     2641 cgaaccacct gaagaaccac caggaacttt tgtctggtcc caagcatttt tagtcggttt
     2701 gaaggcagag ttctcattag aaccacccat agcaaattcg tccatgttgg tcttaccaac
     2761 aacaatcata tccttggcat aggcttggga aactgatgtc gcatcgaaaa tcggctcgta
     2821 attgtagagc atttttgaag ctgccgtggt caaaatcccc ttagtagaga tattgtcctt
     2881 aactgccaga ggaatccccg ccataacatt gtccgcatca attccctttt catcaagggc
     2941 agcagcctgc gccaaagctg catcttctgt aatagtcaag aaagcatcca ctgctccctc
     3001 acggctcttg atgtcttcca aggttgcctt ggtcaactca accgcagaaa tccccttttt
     3061 gacaaggagg tcatgcaatt catcaatggt tttattgtta aaagtcatta ggcatctcct
     3121 cccccgtcta gaatagctgg taccttgata aagttatctt gtgattcagg cacattttta
     3181 aagagctcct cacggctctc acctttttgg gcaatatctg ctcgcaagac attcttacgg
     3241 tcagccatag tggttgttac cgcaacacct gtcgtatcta cttcattgag caactcaacc
     3301 atatcaacaa tcttgctcaa acttgtcgca aattccgctg tttcttgatc cgaaaattcc
     3361 agcttagaca gcttggcaac gtgacggact tcagcttcag aaatcttcat tcttactttc
     3421 ctcctaatgg aaatctatct tctcattata ccatatttcc agccccagac aaaggtagaa
     3481 atgacctaat atgttaccaa tttcctccta aaaatccgtt ctttaaattg cgaagtattc
     3541 ctaatctcac accgtaaaac gataaaaaac agcaagtttc atctagcaat aaaacttact
     3601 gtttgcttag cctatctttt tatgagctaa tcctttgcat gtttgcggta tttttccatt
     3661 ttccctttaa aaagctcccc attacttggt gctgtatagg agacagcatt ggctccagct
     3721 tcgataaccc gacgaataga gtcctcagtc ggaccaccag tcgcaataat tggaaagtca
     3781 gggaaagctt ctcgaatctt cttcacaacc cttggcgttt ccataccgca agaaacattt
     3841 aaaatatcta ctccagcttt aatccgtgag gttaaatcag acttttcaga aacaacggta
     3901 taaatgatag gaatatctac taccttattg attgcctcaa ttgtttttac atccgttggt
     3961 ccatttacca caacagcgta tgctccctcc gactcagaca agagactcat attagctgaa
     4021 cgcatacctg tcgtcaagcc tccaccaaca ccagccaaga ctggaactga cgcaaccaac
     4081 ataatacttt tgataatagc tggactgggc gtaaatggat aaacagccaa aatagcatct
     4141 gcattgtgat tggcaataat agacacatcc gttgtaaata agactgaacg aatgcgacga
     4201 ccataaatac gaattccact acattccttg ataacttctg gcatctgaac aatatcctgc
     4261 cgtaaatctg ataaaacaga tggaataaac tgttcgctca tccgcaacct cctctatact
     4321 agctacaata atatatagta taacatatta tttcatggaa gatactcaaa aacaatccat
     4381 tttaccactg ctttcacaat ttggtactct ttcaggctct ataatttctg tagtgggtaa
     4441 atccactgta gagattatgg agcctttttg agtatataca aaaagtccca tatgacctat
     4501 aatgaaaagc aaccacactc acattagaaa gactcatatg gaacaacttt atcataccac
     4561 agaactaatt ggaatcaaag acaaaaacat caaaattctc tttattttga aacatcagac
     4621 ccatctagaa atcagggcaa agctagatta cgacagccct ggttgtcctc attgccaaag
     4681 aaagtgtatc aagtacgact ttcaaaagtc gtcaaagatt ccgattttgg attgtcaggg
     4741 ctttccgacc ctcctcttgc ttaagaaacg gcggtttcaa tgtaaatctt gccaaaaagt
     4801 gactgtagct gagacagctc tagtcaagaa aaactgtcag atttccctac ctatttggga
     4861 gaaagtgaca caactccata cagaaaacat gaccaatata gccatcgcta ggagattaca
     4921 tatctccgta tcggtcctcc agaagaaact agctcagttt caattggagc ataattttac
     4981 gaaattacca aaagtcttga gctgggatga atttagtcgg aacaaaggaa aacttgcctt
     5041 tattgctcag gattttgaga cgagacgcat tgtgaccatt ctagagaata accgccaaac
     5101 caccatcaag aactatttct acaagtatcc tagggcagtc agagaaactg tcgaggtcgt
     5161 aacagtggac atgtcgggta gctatattcc catcatcaaa aaactatttc caagagctaa
     5221 aattgtcctt gatagatttc acattatcca acacctgagc cgtgctatga tgacgactag
     5281 aattgatatt atgaagactt tcgatacgcg ttccctacct tatcgatcca
//
LOCUS       DATUCG010000141         5241 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.143, whole genome shotgun sequence.
ACCESSION   DATUCG010000141 DATUCG010000000
VERSION     DATUCG010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5241
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.143"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(72..896)
                     /gene="nadE"
                     /locus_tag="VCA20_002106"
     CDS             complement(72..896)
                     /gene="nadE"
                     /locus_tag="VCA20_002106"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEP1835849.1"
                     /translation="MTLQETIIKELGVKPSIDPKEEIRRSIDFLKDYLKKHPFLKTYV
                     LGISGGQDSTLAGRLAQLTMEEMRAETGDDSYQFIAIRLPYGVQADESDAQAALAFIQ
                     PDVSLTINIKESTDAMVAAVNANGLAMSDFNKGNAKARMRMIAQYAIAGERKGAVIGT
                     DHAAENITGFFTKHGDGGADILPLFRLNKRQGKQLLAELGADEKLYLKVPTADLEEDK
                     PGLADEVALGVTYNQIDDYLEGKTIDPQSQTIIEGWWNKTAHKRHLPITIFDDFWK"
     gene            complement(909..2369)
                     /locus_tag="VCA20_002107"
     CDS             complement(909..2369)
                     /locus_tag="VCA20_002107"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEP1835850.1"
                     /translation="MTLFKDDSLTLHTDLYQINMMQVYFDQNIHNKHAVFEVYFRSQP
                     FKNGYAVFAGLERVVNYLNNLSFSQTDIDYLTELGYPQDFLDYLSQLEMTLTVRSAKE
                     GDLVYANEPILQIEGPLAQCQLIETALLNIVNYQTLIATKARRIKAVIEDEPLMEFGT
                     RRAQEMDAAIWGTRAAFIGGASATSNVRAGKIFGIPVAGTLAHALVQTYGDDYKAFKA
                     YAETHHDCVFLVDTYDTLRIGVPAAIKVAKEMGDKINFKGVRIDSGDMAYISKKVRQQ
                     LDDAGFTDAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVS
                     IEDEDGNMRDTIKLSSNAEKVSTPGKKQVWRITSKEKGKSEGDYITFADTDVTQMDNI
                     YMFHPTYTYINKTIENFDARPLLVPIFEKGKQVYELPSLAEIQTYANQEFDKLWDEYK
                     RVLNPQLYPVDLAQDVWDNKMNLINRIRKQTQTKSI"
     gene            complement(2477..3121)
                     /locus_tag="VCA20_002108"
     CDS             complement(2477..3121)
                     /locus_tag="VCA20_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEP1835851.1"
                     /translation="MKTIILFFLGASIGSFLGLVIDRFPEQSIIASSSHCNACKRRLK
                     AWDLIPILSQLSTKSKCRYCKAKIPYWYLGLEFLAGLVVLLCHFQVLNLTETILILAG
                     LVLTIYDIKHQEYPFAVWLVFTFIALVLSQLNWLFCGFLLLAYLTEKWQINIGSGDFL
                     YLASLALICGFTELLWIIQISSLLGLLVFAIFKPKSIPYVPLLFLSSIPIILCI"
     gene            complement(3171..3989)
                     /locus_tag="VCA20_002109"
     CDS             complement(3171..3989)
                     /locus_tag="VCA20_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEP1835852.1"
                     /translation="MSSSKNTFIAFLLNISFSIIEFIFGWLFGSSAILADAVHDLGDA
                     LAIGSSAIMQTYANKPADRVYPFGYKRWNILAALLTSSILIIGSSLILVENIPNLFHP
                     QPINQAGMLALGIIAVIINFLASRIVKNGHSHNESILSLHFLEDILGWLAVILVSIIL
                     QFTDWYFLDPLLSLCIAIFILSKALPKAWNNVMILLETSPQGVNIEEVKGVLLALENV
                     HEITQLNVWTMDGYEHCATMTVKLTNPAVTDKTKAKIRNSLSTFGIIQNTIEIL"
     gene            4111..4629
                     /locus_tag="VCA20_002110"
     CDS             4111..4629
                     /locus_tag="VCA20_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEP1835853.1"
                     /translation="MDRRIAKTKRAIFQAFLTLLNDKGYDHMRVQDVIDLADVGRSTF
                     YAHYASKEALLEELCHDLFHHLFTGREDADVKTLLVHIFKHFRTNQDRVASLLLSRNA
                     YFLRELEAELKHDIFPKVAEDYMQDKRNLPEDLLVHFVVTTFVETVSWWLQQRKKVDE
                     ATLTDYFIRLLT"
     gene            4639..5184
                     /locus_tag="VCA20_002111"
     CDS             4639..5184
                     /locus_tag="VCA20_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002899762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEP1835854.1"
                     /translation="MLRPLHMAHAFLDEILTDQDLAVDATMGNGHDTLFLAQRAGKVV
                     AFDIQEQALTTTAEKLEKAGLTNAKLVLTGHEKLDQYVEECKAAIFNLGYLPSADKSV
                     ITLPATTLQAIEKVLDRLVVGGRLAIMIYYGHEGGVLEKDAVLDFISQLDQTVFTAML
                     YKPLNQVNTPPFLVMVERLKK"
BASE COUNT         1477 a         1007 c         1270 g         1487 t
ORIGIN      
        1 tctttaggcg ctggctcgtg atgtgggctt atagcccttg ttcaaaccac ccgtttgacg
       61 ggtggtcatg attatttcca aaagtcatca aaaatagtaa ttggcaggtg gcgtttatgg
      121 gctgttttgt tccaccatcc ttcgataatg gtttgggatt gagggtcgat ggttttgcct
      181 tcgagatagt cgtcaatttg gttataggtt acaccgaggg cgacttcgtc agcaagacct
      241 ggcttgtctt cttccaagtc cgcagtaggg acttttaggt agagtttttc atctgctccc
      301 agttcagcta gaagctgttt tccttggcgt ttgttaaggc ggaagagggg gagaatgtct
      361 gcaccgccgt cgccgtgctt ggtaaagaaa ccagtgatat tttcagcggc atggtcggtt
      421 ccaataacag cccccttgcg ttctccagcg atagcgtatt gggcaatcat gcgcatgcgg
      481 gctttagcat ttcccttatt aaaatcagac atagcaagcc catttgcatt gactgcagcc
      541 accatggcat ctgttgattc cttgatgttg atggttaagc tgacatctgg ttgaataaag
      601 gctagggctg cttgggcgtc ggactcgtca gcttgcacgc cgtaaggtag gcggatagcg
      661 ataaattgat aagaatcgtc acctgtttcc gcccgcattt cttccatagt cagctgggcc
      721 aaacgaccag ccaaggtcga gtcttgtcca cccgaaattc ccaagacata ggttttcaaa
      781 aatgggtgtt ttttcaggta atctttgaga aagtcgatgg agcgacggat ttcttctttg
      841 gggtcaatgc ttggcttgac acctaattct ttgatgattg tttcctgtag ggtcataaca
      901 tacctttctt agatggattt ggtctgagtt tgtttgcgaa tacgattgat gaggttcatt
      961 ttattatccc aaacatcttg tgccaagtcg actggataga gctgagggtt gagcacgcgt
     1021 ttgtattcat cccagagttt atcaaattct tgattggcat aggtttgaat ttccgctagg
     1081 cttggaagtt catagacctg ttttcctttt tcgaaaatag gaactaggag aggacgtgca
     1141 tcaaagttct cgatggtttt attgatgtag gtgtaagttg gatggaacat atagatatta
     1201 tccatctgtg tgacgtcagt gtccgcaaag gtgatgtagt ccccttctga ttttcctttt
     1261 tctttggagg tgatgcgcca aacctgtttc ttaccaggag tggagacttt ttccgcatta
     1321 gaagacagct tgatggtgtc acgcatattt ccatcttcat cttcgatgga tacaatcttg
     1381 tagacagcgc ccaaggcagg ctggtcgtag gcggtgatga gtttggtacc aactccccag
     1441 acatcaattt ttgccttttg cattttgagg ttgaggatgg tattttcatc caggtcatta
     1501 gatgcgtaaa tcttcgcatc tgtaaaacct gcatcatcta attgctgacg aactttcttg
     1561 gaaatatagg ccatatcgcc tgaatcaatc cgaacccctt tgaagttaat cttatctccc
     1621 atttctttgg caaccttgat ggctgctgga acaccaatac gaagtgtatc ataggtatca
     1681 acgaggaaga cacagtcatg atgggtttca gcgtaggctt tgaaagcctt gtaatcatct
     1741 ccgtatgttt gaaccaaggc gtgggcaagg gtaccagcga cagggatgcc gaagattttg
     1801 ccagctcgaa cattggaagt cgcagatgct ccaccaataa aggctgctcg agtcccccag
     1861 atagcagcat ccatttcttg ggcacggcga gtgccaaatt ccatcaaggg ctcatcttca
     1921 atgacagcct tgatacggcg agcttttgtg gcaattaggg tttggtagtt gacaatgttt
     1981 aagagggcag tttcgattag ttggcattgt gccaatggtc cttcgatttg aagaataggt
     2041 tcgttagcgt agactaggtc gccctcctta gccgaacgga cagtgagagt catttccaat
     2101 tgggacaagt aatcaaggaa atcctgcgga taacctagtt ctgtcagata gtcaatgtct
     2161 gtctggctaa agctgagatt gttgaggtag tttacaacgc gttccaaacc ggcaaataca
     2221 gcgtagccat ttttaaaagg ttgtgaacgg aaatagactt cgaatacagc gtgtttattg
     2281 tggatgtttt ggtcaaaata gacctgcatc atgttgattt gataaaggtc ggtatgcagg
     2341 gttaaactgt catccttaaa tagtgtcata acgtttctcc ttttgccttt caatcaagaa
     2401 ggcactccaa ttattttgat ggagttgaac tctaatatgc cttctattat aacaaaaaaa
     2461 tcgagaaaaa ctcgatttag atgcacagaa tgataggaat acttgaaaga aaaaggagtg
     2521 gtacgtaggg aatagacttg ggtttgaaaa tagcgaagac aagaagccct aggagggaac
     2581 taatctggat aatccagagg agttctgtaa atccacatat taatgccaaa cttgccagat
     2641 agagaaagtc accagaacca atattgattt gccatttttc agtcagatag gccaagagta
     2701 aaaagccaca gaaaagccag ttgagctggg agagtaccag agctataaaa gtaaaaacaa
     2761 gccagacagc aaaaggatat tcctgatgtt tgatgtcgta aatggtcaaa actagtcctg
     2821 ccaggatgag aatggtttcg gttaggttta ggacttgaaa atggcagagc aggacaacta
     2881 gaccagctaa gaattctagt cccagatacc aataaggtat cttcgccttg cagtaacggc
     2941 atttggattt tgtcgaaagt tgggacagga tgggaattaa gtcccaggcc ttgagccgtc
     3001 gcttgcaggc attgcagtga ctagaggagg cgataatgga ctgttcaggg aaacggtcga
     3061 tgaccaatcc caagaaggag ccgatagaag ctcccaggaa aaataggata attgtcttca
     3121 tacctattta ttcgcaaatg gatggaggaa tatgaagctt tttaaaattt ttacaaaatt
     3181 tcgatggtgt tttggataat gccaaaggtg ctaagagaat tccgtatttt tgctttggtc
     3241 ttgtctgtta cagcaggatt tgtcagtttg accgtcatag tcgcacaatg ttcatatcca
     3301 tccatggtcc agacattcag ttgagtgatt tcgtgaacat tttcaagagc caggaggact
     3361 cctttcactt cttcaatatt tactccttgc ggacttgttt caagtagaat cataacattg
     3421 ttccaggctt ttggcagggc tttggagagg atgaagatag caatacagag cgagagaagt
     3481 gggtctagaa agtaccaatc agtaaattga agaatgatgg aaacaaggat gaccgccagc
     3541 catcccaaaa tatcctctaa aaagtgcaag ctgagaatgg attcattatg gctatggcca
     3601 ttttttacaa tacgggaagc aaggaaattg atgatgacag ctataatccc aagtgccagc
     3661 atcccagctt gattgatagg ctggggatgg aaaagattgg gaatgttttc cacaaggatt
     3721 aagctggagc cgataatcag aatgctagaa gtcaagagag cggccaaaat attccatctc
     3781 ttgtagccaa agggataaac cctatcagca ggcttgttgg cataggtttg catgatggca
     3841 gagcttccga tggcgagggc gtcacccaaa tcatgaacag catctgctaa gatggcgctg
     3901 gaaccaaaga gccagccaaa gataaactcg atgatagaaa agctgatatt gaggagaaaa
     3961 gcgataaatg tatttttaga agatgacatg agattccctt tctgtgttat actgtttata
     4021 ggtttattat gcactgtaat gagccgaaaa actagaaaca agagaaacgg tttgtctttt
     4081 tctgaacaaa ttgtttgaaa tgtacgaaaa atggatagac gaattgcaaa aacaaaaaga
     4141 gctatttttc aggctttttt gaccttatta aatgataaag gctatgacca tatgcgcgtg
     4201 caggatgtca ttgacctggc agatgtgggg cgttccactt tctatgctca ctatgccagc
     4261 aaggaagcac tgttagaaga actttgtcac gacctctttc atcacttgtt tacaggtcgt
     4321 gaagatgcgg atgtgaagac cttgttggtt catattttca aacactttcg gaccaatcaa
     4381 gatcgtgttg ccagcctttt gttgtcccgc aatgcttatt ttctaagaga actggaagcg
     4441 gagttgaaac acgacatttt tccaaaagtg gctgaagatt acatgcagga taagaggaat
     4501 cttccagagg atttgctggt gcattttgtt gtgacgactt ttgtggagac ggtcagctgg
     4561 tggctccagc aacgcaaaaa ggtggacgag gccaccttga ccgattattt tataagatta
     4621 ttaacttgag gagaaagtat gcttagacct ttacacatgg cccatgcctt tttagatgaa
     4681 atattaacag accaggacct agcggtcgat gcgactatgg gaaatggcca tgacacgcta
     4741 tttttagccc agcgagcagg aaaggttgtg gcttttgata ttcaggaaca agccctgacc
     4801 acaactgctg aaaaactaga aaaagctggc ttgaccaatg ccaaattggt tttgactggg
     4861 cacgaaaaac tagaccagta tgtggaggaa tgcaaggcag cgattttcaa tctgggctat
     4921 ctaccttcgg cggataaatc agtcatcacc ctgcctgcaa ctacccttca ggccattgag
     4981 aaagtcctgg atagattggt ggttggaggt cgcttggcta tcatgattta ctatggtcac
     5041 gagggtggag ttcttgagaa ggatgcagtt ttagacttta tcagccagct agaccaaacc
     5101 gtctttacag ccatgcttta caaaccgctt aaccaagtca ataccccacc atttttggtg
     5161 atggtggaaa gattgaaaaa gtaggaaaaa aattatataa ttgttgaaat attacttgac
     5221 ataggagaac gaaatggtac a
//
LOCUS       DATUCG010000142         5197 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.144, whole genome shotgun sequence.
ACCESSION   DATUCG010000142 DATUCG010000000
VERSION     DATUCG010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5197
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.144"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            123..569
                     /locus_tag="VCA20_002112"
     CDS             123..569
                     /locus_tag="VCA20_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEP1835855.1"
                     /translation="MEQTISAAEWQVMRVLWAHPGATSQEIIQALQEGFDWQATTIKT
                     LLGRLRKKNYLRMAKETSKYHYYPLISEEEHLQWQVELLLATVCSTRQGQLVEKLLDT
                     GIFSQKSLENLASKILQLQRTASEQIACRCLAGQCTCGHHAKGEKT"
     gene            566..775
                     /locus_tag="VCA20_002113"
     CDS             566..775
                     /locus_tag="VCA20_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEP1835856.1"
                     /translation="MKQTLKLKNLSCQNCVKHVTNHFLDLDGVEEVKIQLEEQLAEVE
                     TSVAYDLEQYQEVLDDTIYEVEELV"
     gene            complement(803..1180)
                     /locus_tag="VCA20_002114"
     CDS             complement(803..1180)
                     /locus_tag="VCA20_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEP1835857.1"
                     /translation="MTCIFCHQLKEEDILYQTEHFKVVWDVDPVQTGHLLIISKEHYD
                     TLSQVPPTVRYELSDLEVFLTEKLWQTLAIDGVTTACNDRLFDAGTHFHVHLIPRFRS
                     DGFWDQISLAQAQLDLTHFLKAL"
     gene            complement(1237..2493)
                     /locus_tag="VCA20_002115"
     CDS             complement(1237..2493)
                     /locus_tag="VCA20_002115"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEP1835858.1"
                     /translation="MNIFEELKARGLVFQTTDEEALVKALTEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSFQTKDTVDGWVTKIQ
                     GQLSRFLDFENGDNKAEMVNNYDWFSDISFIDFLRDVGKYYTVNYMMSKDSVKKRIET
                     GISYTEFAYQIMQGYDFYELNDKHNVTLQIGGSDQWGNMTAGTELLRRKADKSGHVMT
                     VPLITDSTGKKFGKSEGNAVWLDADKTSPYEMYQFWLNVMDDDAVRFLKIFTFLSLDE
                     IAKIEEEFDAARHERLAQKILAKEVVTLVHGEEAYNQALNITEQLFAGNIKNLSAKEL
                     KQGLSNVPNYAVQAEDNLNIVELLVTSGIVTSKRQAREDVANGAIYVNGERVQDLDYT
                     LSDSDKIDGELTVIRRGKKKYSVLTY"
     gene            2647..5049
                     /locus_tag="VCA20_002116"
     CDS             2647..5049
                     /locus_tag="VCA20_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEP1835859.1"
                     /translation="MATKSDKQDFKKKISALGLGDVVGVFLRTLKLLFNSVAVLVFLF
                     GLFGAGIGIGFVVSLFDDVKIPKTEELVAKVSEVSRISTVTYSDGSLVSEVNSDLLRV
                     PITSEEVSNYLKQAVIATEDETFENHNGVVPKAVLRAALGSVGLGSSSGGSTLTQQLI
                     KQQLVGDAPTFTRKANEIVSALALERNMTKEEILTIYLNVSPFGRNNQGRNIAGVEAA
                     AQGIFGKPAKDLTVPQAAFIAGLPQSPIVYSPYASDGTRKSDEDMVYGIERYQDVLFN
                     MYRASFLTKEEYETYKAYDIKQDFIAPAPVMADTKDYLYYEVMEEAQEVMFDYLVKRD
                     KVSKNDLKNDETKASYEELAKQELSQGGYTIKSTVDQKIYAAMQSVVANYGSVLDDGN
                     EYVETGSVLIDNATGAILGFVGGRDYATNQNNHAFDTLRSPASTIKPLLAYGIAIDQG
                     LIGSASILSDYPTNFSSGQPIMYGSGRGTGMMNLQTAIDRSVNIPAFWTYKMMRNAGV
                     DAKAYMDKMNYHIPMYDIESVPLGGGVEISVLTNTNAYQTLANGGVYNKHYIVESITA
                     SDGTVVYQHEAAPVQVYSKATASIMNQLLRQVVNSGYTTTFKSRLSGLNPQAASSDFV
                     GKTGTSNEVNDVWLMLSTPRVTLGTWAGNDDNSEMYVWTGYHNNSQYVAHMVDALYNV
                     NADMFAGKFELDSSVIASSVVASTGQRAGTTQVNGRQVTVGGAMTTSYWVKNGAPVTS
                     YNFMVGGTDSDRAQAWNTIIGSQTPTSSSSTQRSSSTRSSTSSSANNTNQSSETQTAT
                     SE"
BASE COUNT         1503 a         1015 c         1242 g         1437 t
ORIGIN      
        1 aataaattct aaggactttg tcttcagtct gaggctgaac ggaatgttca gtctttcttc
       61 ttgacaattt ttgactacaa atgtaaacta aagttagaaa attattgttg ggaggtgaat
      121 gtgtggagca gacaatttct gctgcggagt ggcaggtcat gcgggttctg tgggcccatc
      181 ctggtgcgac ttctcaggaa attattcagg ccttgcagga aggctttgat tggcaggcga
      241 cgaccattaa gacactcttg gggcggttgc ggaagaaaaa ctatttgaga atggccaagg
      301 aaaccagcaa ataccactat tatccgctga tcagtgaaga ggaacatttg cagtggcagg
      361 tggagctctt actagctacc gtatgttcca caagacaggg gcaattggtt gaaaaactgc
      421 tagacacagg gatattttcc caaaaaagcc tagaaaatct agccagcaaa atcttgcagt
      481 tacaaaggac tgcatccgag caaatcgctt gtcggtgctt ggcggggcaa tgtacctgtg
      541 ggcatcatgc aaaaggagaa aaaacatgaa gcaaactttg aaattgaaaa acttatcgtg
      601 ccaaaactgt gtcaagcatg tgaccaatca cttcttggac ttggatgggg tggaagaggt
      661 gaaaatccag ctggaagagc agttggcgga ggtggaaacc tctgtagcct atgacttgga
      721 acagtaccaa gaggtgctgg atgatactat ctatgaagtg gaagagttgg tataagcgaa
      781 aaagcagccg tcgggctgct ttttataatg ctttgagaaa atgagtcagg tctagttgcg
      841 cttgcgcaag tgaaatttgg tcccagaagc cgtctgatcg aaagcgtgga attaggtgga
      901 catggaaatg ggtgccagca tcaaacaagc gatcgttgca ggctgtggtc acaccgtcaa
      961 ttgccaatgt ttgccagagt ttctcagtca aaaaaacttc caagtccgat agttcatagc
     1021 gaacagtagg tgggacttga ctgagcgtgt catagtgttc cttgctgata atcagtagat
     1081 gccctgtttg gacagggtca acatcccaga cgaccttgaa atgttccgtc tggtagagaa
     1141 tatcttcttc tttgagttgg tggcaaaaaa tgcaggtcat gtcttttctc ctaggttagc
     1201 agaaaaggaa ctcacatagg atttccttga aaaatcttaa taagtcaaca cagaatactt
     1261 cttcttccca cgacggataa cggtcagctc gccgtcaatc ttgtcactgt cagaaagggt
     1321 atagtccaag tcctgcacac gctcaccgtt gacataaatg gcaccatttg ctacgtcttc
     1381 acgagcttgg cgttttgaag tgacaatacc agaggtaact agaagttcca cgatgttaag
     1441 gttgtcttca gcctgtacag cgtagtttgg aacgttgctg aggccctgct tgagctcttt
     1501 tgcggacagg tttttgatat tgcctgcgaa caattgctcg gtaatgttaa gggcttggtt
     1561 ataggcttct tcaccgtgaa ccaatgtcac gacttcctta gccaaaattt tctgagccag
     1621 gcgttcatga cgggcagcgt caaattcttc ttcaatctta gcaatctcat ctaatgacaa
     1681 gaaagtaaag attttcaaga aacgaacagc atcgtcatcc atgacattga gccagaattg
     1741 gtacatttca tacggagaag tcttgtcggc atccaaccaa acagcgttgc cttctgactt
     1801 accaaatttc ttgcctgtag agtcggtgat aaggggaaca gtcatgacat gaccagactt
     1861 gtcagccttg cggcgaagca actcggtacc tgcagtcatg ttgccccact ggtcagaacc
     1921 accgatttgt agggttacat tgtgtttgtc attgagttcg tagaagtcat agccctgcat
     1981 gatttggtag gcaaactcgg tgtaggaaat ccctgtttca atccgttttt tcacagagtc
     2041 cttgctcatc atgtagttga cagtgtagta tttaccaaca tcacgaagga agtcgataaa
     2101 actgatgtct gagaaccagt cgtagttgtt gaccatttca gccttgttgt caccattttc
     2161 aaaatccaag aagcgagaga gctgaccttg gatttttgtt acccaaccgt ccacggtatc
     2221 tttggtttga aagctccgct ccgcatcctt gaaggaaggg tcgccaatca gaccagttgc
     2281 accgccaact agggcgtaag gcttgtggcc ggccaactgc aagcgacggc tagttaagat
     2341 agcaaccaag tgtcccaagt gtaaactatc tgctgttgga tcgtatcctg tgtaataaga
     2401 aacctgtcct tctgtcaatg cttttaccaa ggcttcttcg tctgtcgttt gaaagaccaa
     2461 gccacgagct tttagttctt caaaaatgtt catgtgggct gtctccttta ggtttaatat
     2521 acaggaccat aaaccatttt atatgatttt tggaccgaac ttcattttaa aaaattcctc
     2581 cctattatac cacgaaattg cggaaaataa acaaaatttg gtataataga acgataagga
     2641 gttaatatgg cgactaaatc agataagcaa gattttaaaa agaaaatcag tgccctaggc
     2701 ctaggggatg tagtaggagt tttccttcgg acgctgaaat tattattcaa ttcagtagct
     2761 gtcttggtgt tcctattcgg tctttttggt gcgggcatag gaattggttt cgtggttagt
     2821 ctttttgatg acgttaagat tcccaaaaca gaggaactgg tagcgaaggt ttcagaagtt
     2881 agtcggattt ccaccgttac ctattcggat ggtagcttgg tgtcggaagt taattcagat
     2941 ctgcttcgtg tacctattac ttcggaagag gtctcgaatt atctaaaaca ggctgtaatt
     3001 gcgacagagg atgaaacatt tgaaaaccac aatggggttg tgcctaaggc tgttttgcgt
     3061 gcggcactgg gatcagttgg tctaggttca tctagcggtg gttcaacctt gacgcagcaa
     3121 ttgataaaac agcaacttgt cggagatgct ccaaccttta cgcgtaaggc taatgagatt
     3181 gtttcggctt tggcattaga gcggaatatg accaaggaag agattttgac catttactta
     3241 aatgtttctc catttggtcg aaataatcag ggacgaaata ttgcaggggt agaagctgcg
     3301 gctcagggaa tttttggtaa accagcgaaa gacttgactg ttccgcaggc tgcttttatt
     3361 gcaggcttgc ctcaaagtcc gattgtttat tctccctatg catcagatgg aacacgtaaa
     3421 tcagatgaag atatggttta cggtattgaa cgctatcaag atgttctctt taatatgtat
     3481 cgggcgtcat tcttgaccaa ggaagaatac gaaacctaca aagcttacga tattaaacaa
     3541 gattttattg ctccagctcc tgtaatggcg gatacgaaag attatctcta ctacgaggtc
     3601 atggaagaag cgcaagaggt tatgtttgat tacctggtaa aacgcgataa ggtttctaaa
     3661 aatgatttga aaaatgatga aactaaggct tcctatgagg agttggccaa gcaagaattg
     3721 agccaaggtg gctacacgat taagagtacc gttgatcaaa aaatctatgc ggcgatgcag
     3781 tctgttgtgg caaactatgg atctgttttg gatgatggaa acgaatatgt cgagacaggt
     3841 agtgtgttaa ttgataatgc cacaggggcc attttaggat ttgtgggtgg tcgcgactat
     3901 gcaaccaacc aaaataacca tgcctttgat actcttcgtt cgccagcgtc aaccatcaag
     3961 cctctgttag cttacggtat tgcgattgat caaggtttga taggttctgc cagcatcctt
     4021 tccgattatc cgacgaactt ttcaagtgga caaccgatta tgtatggttc aggacgtggt
     4081 actggtatga tgaacttaca gacggctatt gaccgttctg ttaacattcc tgctttttgg
     4141 acctataaga tgatgagaaa tgcaggtgta gatgccaaag cgtacatgga taaaatgaat
     4201 tatcatattc ctatgtacga tattgaaagc gtaccgttgg gcggtggtgt tgaaatttca
     4261 gtattgacaa ataccaatgc ctatcaaact ttggcaaatg gtggcgttta taacaaacat
     4321 tacatcgttg agagtattac tgcctctgat ggaacagtag tgtatcagca tgaagcggct
     4381 ccggttcaag tctattctaa agcaactgca agtattatga accagctttt gagacaggtt
     4441 gtcaactctg gttatactac gacgttcaag agtcgattga gtggtttaaa cccacaggca
     4501 gcatcatctg atttcgtcgg taagacgggg acaagtaatg aagttaatga cgtgtggctc
     4561 atgctatcaa cccctagagt aactttaggt acctgggctg ggaatgatga taactcagaa
     4621 atgtatgtct ggacaggcta ccacaataat tcacaatatg tggcccatat ggttgatgcg
     4681 ctttacaatg ttaatgcgga tatgttcgca ggaaaatttg aattagacag tagtgttatt
     4741 gcttctagtg tcgttgcttc tacaggtcaa cgtgcaggga cgacacaggt aaatgggcgc
     4801 caagtaacag ttggtggtgc gatgacaact agttattggg ttaaaaatgg tgcaccagta
     4861 acgagctata acttcatggt tggggggaca gatagtgacc gagcacaggc ttggaataca
     4921 attatcggtt cccaaacgcc aacatcaagt agctcaaccc aacgaagcag ttcgactcgg
     4981 agtagcacga gttcttcagc taataacacg aaccagtcat cagaaacgca aacagcaact
     5041 agtgagtaag caaataacac gccaaacgca tgaagaaaaa aatcgttttc agaaaacgaa
     5101 aatcataacc aaacatcaat caaagtagta cactagaata gaggtgaact acaatgattg
     5161 attttctttt atccatagaa gagcataaag aagagta
//
LOCUS       DATUCG010000143         5039 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.145, whole genome shotgun sequence.
ACCESSION   DATUCG010000143 DATUCG010000000
VERSION     DATUCG010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5039
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.145"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            64..744
                     /locus_tag="VCA20_002117"
     CDS             64..744
                     /locus_tag="VCA20_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEP1835860.1"
                     /translation="MAKEKARYFTFLLYPESIPSDWELKLELLGVPIAISPLHDRDKS
                     EVEGQKYKKPHYHVIYVSKNPVTADSVRMKIKRSLGDNSVALVQIIRTSIENTYLYLT
                     HESKDAIEKKKYVYDKADITLLSNFDIDRYLTLDVEEKDDMLNEVCDLIDEYDIANMR
                     ELRRFVKLHGAEHGLPSIKIINSVLRSHTGLIRLYFDAVYQERKYGSNIPNVNQETGE
                     ILSEGSDD"
     gene            737..1144
                     /locus_tag="VCA20_002118"
     CDS             737..1144
                     /locus_tag="VCA20_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835861.1"
                     /translation="MTDRNEMINDIVELKAKRDRLLAQVKEAEQWESLAWDSYHAVAD
                     HVKALEKKQEIGQNYWESSQRAISHQFDFVADQANKVKKALVKKRYDLLDEEIDKLMN
                     EVRELADVLGIEINELPFDFPFFALTAERGLDE"
     gene            1137..1742
                     /locus_tag="VCA20_002119"
     CDS             1137..1742
                     /locus_tag="VCA20_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835862.1"
                     /translation="MSKTVSELAQELGLSKQYINRILAQNNLGRKKGNKKVVSGIDEK
                     KLISILEGNVRNKKSETDFIVSDESSIASDKWERRNVGNKKTETEIENSFQYIREQLE
                     IKGKEVEQLHQLLDQSQQLLLNEQKKNQLLLESKNDEFDGWIKEKESFQDRVNRLASD
                     NDYYIRRYYENKSSLKSYKLALLTVSIILVLLVIMYFFFLI"
     gene            2108..3592
                     /locus_tag="VCA20_002120"
     CDS             2108..3592
                     /locus_tag="VCA20_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HEP1835863.1"
                     /translation="MSYMVARMEKMKIGNLGGAYRHNERIFKKHSNKDIDVSRSHLNY
                     ELTDRDRSVSYERQINDYVNENKISNRAIRKDAVLCDEWIITSDKPFFERLSEEKTRD
                     FFETAKNYFAENYGEENIAYASVHLDESTPHMHLGVVPFRDGKLSSKAMFNKEELKKI
                     QDELPKYMGDYGFDLERGELNSEAKHRTVAEFKQEMASKELEKQLVKEYGAPEYINET
                     TGEFYDRIEYHNFTEFPREENLGQVARETTYQEKLGWVKWQQKEQLQTLELSKTPLED
                     EIRALNEVLREKYEELDKIELRSSESLSELSKAEGYINTLEEHSNALEGKIRGLENEY
                     LRLAKQNVRLSDLKIMSEKELAEIQTKKGMFGKEYVELTKEQFDELKGLIYRSRTVIH
                     QKELENNQLKRLVPLRASKNSFEASLERAKEKTKGESIDSLKSEIRGLKNENSILRQQ
                     NDKMFSKLKELMPDKVLNNFISELKSIKPIVKIVKRVIEKGLGL"
     gene            complement(3829..4017)
                     /locus_tag="VCA20_002121"
     CDS             complement(3829..4017)
                     /locus_tag="VCA20_002121"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835864.1"
                     /translation="MKIKSKTIITLTTLGILAFLSSNTLLYQYTGNKISLLGTLLSIP
                     TFFLYLFIHTSVFNKRKK"
     gene            4094..4636
                     /locus_tag="VCA20_002122"
     CDS             4094..4636
                     /locus_tag="VCA20_002122"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835865.1"
                     /translation="MKKKFIFSALGIILLTPVLSTGVVLADALTNQESVVVNNNDISL
                     NVREGETVTLQQIVDGLNVTPQEKEEIIAETLKYVNANNINPENRNMRAYSSARMSTV
                     IKRLSPSQVKKAANSNDWIGYLAGLVPKVGIPLGAIYMLNGQTLRTAANNGWGLELVF
                     TADPNNPTSTGMSFSWRYVK"
     gene            4904..>5039
                     /locus_tag="VCA20_002123"
     CDS             4904..>5039
                     /locus_tag="VCA20_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010889903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEP1835866.1"
                     /translation="MKKRLTITLSESVLENLEKMAKEMGLSKSAMISVALENYKKGQV
                     K"
BASE COUNT         1822 a          691 c         1107 g         1419 t
ORIGIN      
        1 aaaaaaaagc cgagctggca ggcgggcttt taaaagtatt ataacttggg ttaattataa
       61 cttatggcta aagaaaaagc aagatacttc acttttttac tctaccctga gtcaatccct
      121 agtgattggg aattgaaatt agaattgctg ggtgtaccta ttgcaatcag ccctttgcat
      181 gatagagaca agagtgaggt tgagggacaa aaatataaaa aaccgcacta tcatgtgatt
      241 tatgtttcaa aaaatccagt cacggcagat agtgtgagaa tgaaaatcaa gaggtcactt
      301 ggagataata gcgttgctct agttcagata attagaacta gtattgagaa cacgtattta
      361 tatcttacgc atgaatcaaa ggacgctatc gaaaagaaaa agtacgtcta tgataaggct
      421 gatataactt tgcttagtaa ctttgatata gaccgttatt taacgcttga cgtcgaagag
      481 aaagacgata tgctcaatga ggtttgtgat cttatcgatg agtatgatat tgcaaatatg
      541 cgtgaattga gacggtttgt taaacttcat ggggctgaac atggtttacc cagtattaaa
      601 attataaatt cagttttacg ttcgcacact gggttaatac gtctgtattt tgatgctgtc
      661 tatcaagaac gtaagtatgg cagtaatatc cctaatgtta atcaagagac aggtgagatt
      721 ttaagtgagg gtagcgatga ctgatagaaa tgagatgatt aacgatattg tagagttgaa
      781 agctaaacga gacagactac tggctcaagt gaaagaagct gaacaatggg aaagtttagc
      841 gtgggatagt taccatgcgg tcgcagacca tgtgaaagct cttgagaaaa agcaggagat
      901 aggtcaaaac tattgggaaa gttctcaaag agctattagc catcaatttg actttgtggc
      961 tgaccaagct aacaaagtca aaaaggcgtt ggttaaaaag agatatgatt tgctagatga
     1021 ggaaatcgac aagctgatga acgaagttcg agagctggca gatgtccttg ggattgagat
     1081 taacgagctc ccttttgatt ttcctttctt cgctctgact gcagagaggg gtcttgatga
     1141 gtaaaacagt atcagaatta gctcaagaac taggattaag taaacagtat attaatcgga
     1201 ttttagctca aaataatcta ggtcggaaaa agggaaataa aaaagtagtt tccggtatag
     1261 atgagaaaaa acttatatca atacttgagg gcaatgtcag aaacaagaaa tcggaaactg
     1321 attttatagt ttccgatgag tcgtcaattg caagtgataa atgggaaaga aggaatgttg
     1381 gaaacaaaaa aacggaaact gaaatagaaa atagtttcca atatatccgt gaacaattgg
     1441 aaataaaagg aaaagaggtt gaacaacttc atcagttgct tgaccaatct cagcaattgc
     1501 ttttgaacga acagaagaaa aatcagcttt tactggaatc aaaaaacgat gaatttgatg
     1561 gttggataaa agaaaaagag agttttcaag atagagttaa tagattggca agcgacaatg
     1621 attactatat tcgcaggtat tatgaaaata agtcatcttt gaaaagttat aagttagcac
     1681 tattgactgt ctcgataata ttggtcctgc tagtaattat gtattttttc tttttgatat
     1741 aggatgagtc gaccttttgg gttcggcttt tttgtcggac aatagcggag cggtccgact
     1801 agtcattttg aaaaaatgac tttggtcgac ttgaggaagt cgtccaacat gataaaaatg
     1861 tccttgtgtt tcgaaggtgt ttttttatct cgccagcttt cttttgtggc gagcgagctg
     1921 tctgcaagac ccttcggaga acgcacattt acgaagtaaa gtataatgcg ttatacttta
     1981 cttggaagtg gcaccgataa ttgtgctaga atacaagtta agttatttcg tattttagac
     2041 gagcaataga cttgtcggct cgtctgtcag tcgttagtcg actgacacag gtaagggggc
     2101 taagataatg agttacatgg ttgcaagaat ggagaaaatg aaaattggta atttaggagg
     2161 agcttataga cacaatgaac gtatttttaa gaaacactca aataaagata tagacgtgag
     2221 caggtcacac ctcaactatg agttgacgga tcgtgaccgc tcggtgtcgt atgaacggca
     2281 aattaatgac tatgtgaacg aaaataaaat atctaatcga gctattcgga aagatgctgt
     2341 tttgtgcgat gaatggatta tcacgtctga taagccattc tttgagagac tgagtgagga
     2401 aaaaacgaga gatttttttg agacggctaa aaattatttt gctgaaaact atggcgaaga
     2461 aaatatagct tatgcgagtg tacaccttga tgagagtact ccgcatatgc acctgggagt
     2521 agtaccgttt cgagacggaa aattatcgtc aaaggcaatg tttaacaaag aggaactcaa
     2581 gaaaatacaa gatgagttac caaaatacat gggcgattat ggctttgatt tggagcgtgg
     2641 tgagctaaat agcgaagcta aacacaggac ggttgctgaa tttaagcaag aaatggctag
     2701 taaagaactt gaaaaacagt tggttaaaga atatggagca ccagagtaca ttaacgaaac
     2761 gacaggtgaa ttttatgacc gtattgaata ccataatttc actgaatttc cgagagaaga
     2821 aaacttagga caagtagcta gagaaacaac gtatcaagaa aagttgggtt gggtaaaatg
     2881 gcaacagaaa gaacagctac aaacgttaga attgtctaaa acgcccctag aagacgaaat
     2941 aagagctttg aatgaagtgc ttagggaaaa atacgaagaa ttggataaaa tcgagctacg
     3001 atcttctgag agcctctcag agctatctaa agccgaaggg tatataaata ccctagaaga
     3061 acattcaaat gccttagagg gtaaaataag gggtttggaa aacgaatatt taagactagc
     3121 taaacaaaat gttcgattaa gtgacttgaa aatcatgtct gaaaaagaat tggctgaaat
     3181 tcagacgaaa aaaggcatgt ttggaaaaga gtatgttgaa ttgactaagg aacaatttga
     3241 tgaattgaaa gggttgatat atcgaagtag gactgtaatt catcaaaaag agttagagaa
     3301 taatcaatta aaacgcttag tgcctttgag agcctccaaa aatagctttg aagctagttt
     3361 agaacgagct aaggaaaaaa ctaaaggtga aagtatagat agcctaaaaa gcgaaattag
     3421 aggtcttaaa aacgaaaatt ctattttaag gcaacaaaat gacaagatgt ttagtaagtt
     3481 aaaggaatta atgcctgaca aagtgttaaa taattttatt tctgaactta aatcaatcaa
     3541 acctatcgtg aaaatagtta aaagagttat tgaaaaaggt ttaggacttt aagcgattta
     3601 tgccgagaaa actcttgcta ggaagctatg cgaaatagac tagtaggggt cgagtcgaca
     3661 ggctgaaagc ttgacggccg aacacgacag tctgatttca gcataggtta ctttctagca
     3721 agaggaaatt ggaataaagt aagcgaaaat atggaactga agaagtaaag tcgatttttt
     3781 ggtcataatt gaaaaatctg agaaaataaa aagcccaata acggcttttt atttttttct
     3841 tttgttaaat actgaagtgt gaataaaaag atatagaaaa aatgttggaa tcgagagtaa
     3901 agtacctagt aatgaaattt tattacctgt atattgatat aatagtgtat tactacttaa
     3961 aaaggctaaa atccctaatg ttgttaaagt tattatagtt ttagatttta ttttcataag
     4021 aacctcctta acatttggat acgctttcat tttattgtat aatagttaaa aaatcaaatt
     4081 ggaggtgtat atcatgaaga aaaaatttat cttcagtgct ttagggatca ttttattgac
     4141 tcctgtattg tcgacaggtg ttgttcttgc tgatgcttta actaatcaag aatcagtagt
     4201 tgtaaacaat aatgatattt cgttaaatgt tcgagaaggt gaaacggtca cacttcagca
     4261 aatagtagac ggtttgaatg taactcctca ggaaaaagaa gaaattattg cagaaactct
     4321 aaaatatgta aatgcaaata atattaatcc agaaaataga aatatgaggg cttatagtag
     4381 tgcaagaatg tcaacggtta ttaaaagatt aagcccttca caagttaaaa aagcagctaa
     4441 tagcaatgat tggattggtt atcttgctgg tcttgttcca aaagttggga tacctttagg
     4501 agcaatatat atgcttaatg ggcaaacttt aagaacagca gccaataatg gttggggatt
     4561 ggaacttgtt tttacggcgg acccgaataa tccaactagt acaggtatgt ctttctcttg
     4621 gagatatgtt aaataatata aaaaaataaa tctaggaaga ttgagagggt tttctagttt
     4681 tagggaaaaa ggggggtctt actacgaccc cccttttaag tgccgagtgc caaattgcga
     4741 caaaaagtgc catgaaacta gatatatcaa ggttttagtt catgttacac ttcggcgaca
     4801 tttcggcgac atttttggcg acaaaaagta aaaaatacga aaaatgttgc aatgaatgca
     4861 cagttatgct actatggttt tataaattgt tgagaggtgg aacatgaaaa aaagattgac
     4921 gataacactc agtgaatcgg tgcttgaaaa tcttgaaaaa atggcaaaag agatggggtt
     4981 gtcaaaatct gcaatgattt cagttgcctt ggaaaactac aagaaaggtc aagtaaaat
//
LOCUS       DATUCG010000144         5034 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.146, whole genome shotgun sequence.
ACCESSION   DATUCG010000144 DATUCG010000000
VERSION     DATUCG010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5034
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.146"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            402..1399
                     /locus_tag="VCA20_002124"
                     /pseudo
     CDS             402..1399
                     /locus_tag="VCA20_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009660198.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1425..1640
                     /locus_tag="VCA20_002125"
                     /pseudo
     CDS             1425..1640
                     /locus_tag="VCA20_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002959562.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AIR carboxylase family protein"
     gene            1674..2759
                     /gene="purK"
                     /locus_tag="VCA20_002126"
     CDS             1674..2759
                     /gene="purK"
                     /locus_tag="VCA20_002126"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEP1835867.1"
                     /translation="MTSSKTIGIIGGGQLGQMMAISAIYMCHKVITLDPAADCPASKV
                     SEVIVAPYHDVVALKQLADRCDVLTYEFENVDADGLDAVIKDGQLPQGTDLLRISQNR
                     IFEKDFLAKKAGVQVAPYKVVTSSLDLEGLDLSKNYVLKTATGGYDGHGQKVIREEAD
                     LVEASQLANSAECVLEEFVNFDLEISVLVSGNGSDYTVFPVQENIHRNNILYKTIVPA
                     RISDELAEKAKTMALQIADKLQLAGTLCVEMFVAGQEILVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHIRGILGEPLPPIRLLSPAVMINVLGQDMEAAQTFLQENPTAHPHFYGKLE
                     AKHNRKMGHVTVLTDDVEVGFRQDEIR"
     gene            2746..3669
                     /locus_tag="VCA20_002127"
     CDS             2746..3669
                     /locus_tag="VCA20_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4268 domain-containing protein"
                     /protein_id="HEP1835868.1"
                     /translation="MKLDRLKEVDVREIWKHEQYDFSEWLSRTENIELLSDEIGLTLT
                     EIDKEVFVGSYRCDIVAIDETTGIKVIIENQLELTDHDHLGKIITYASGLDAEVVIWI
                     VKKAREEHRSAIEWLNNNTTKNISFFLIEIHAYQIGSSMPAPKFVLVEKPNDFTKLAK
                     GNNHGGDLSKAQAERLYFWTQFNNVVATKNKPFNIRKATTDHWYDVAIGTSEAHISIT
                     LVNKSKSVGIEIYINNNKELFDKLSSHADEIHEELGFEMEWKRLDNRKVSRIIYYIKG
                     LDFENHDNYNDLIDEVINKVIILRDVVKKYI"
     gene            3704..4996
                     /gene="purB"
                     /locus_tag="VCA20_002128"
     CDS             3704..4996
                     /gene="purB"
                     /locus_tag="VCA20_002128"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEP1835869.1"
                     /translation="MIERYTRPEMAAIWSEENKYKAWLEVEILADEAWAELGEIPKED
                     VALIREKATFDIDRILEIEEETRHDVVAFTRAVSESLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIIADKAREHKYTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNMERFDVAAKGVEAGKISGAVGNFANIPPFVEEYVCGKLGIRPQEISTQVLPRDL
                     HAGYFSALALIATSIERMATEIRGLQKSEQREVEEYFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVVRGHLVTAFENVALWHERDISHSSAERIITPDTTILINYMLNRFGNIVKNL
                     TVFPENMKRNMESTFGLIYSQRVMLSLIEKGMTREEAYDLVQPKTAHSWDNQVDFKPL
                     LEADERVTAKLSQEEIDELFNPDYYAKRVDDIFERLGL"
BASE COUNT         1464 a         1033 c         1216 g         1321 t
ORIGIN      
        1 agagattacc attttatgtt tattttcttc gagtccagtc aatgaaaata ttaacataat
       61 tacggctacc acattcatga taaaaaaaca atatcccatt tattactgta tgagtatagt
      121 cgttggctag caataatgcg attagttctt gttttatcaa gagtttcaat tttatgttga
      181 atttcttcct taatatttct tgcgctcatt tataacaatc cttataaaaa tttttaatgt
      241 aattataaca cactaataat attttgcaaa tacaatgtgg ttttacaaaa agttagtcat
      301 tttaataagt tccattcccg aacgttttcc tgttttttct atcaaaatcc gtctgttttt
      361 actatactgt ttatataaaa tccgtttttg aggtgtgaaa aatgaaaatt ttggtcgttg
      421 ggtctggtgg ccgtgaacat gctatcgcaa agaaattgtt agaatctaag caggtagagc
      481 aggtctttgt cgctcctgga aatgacggaa tgactttaga tggaatcgag ttagtcaata
      541 tcggaatttc cgaacattct gctctgatta actttgctaa ggaacatgat attgcttgga
      601 cttttgtggg tccagatgac gctctggcag caggtatcgt cgatgatttt gaagaggctg
      661 gactaaaagc ttttggtccc agtcgtctag ccgcggagct ggagtggtca aaagactttg
      721 tcaaacaaat catggtcaaa tacggcattc caacagcagc ctttggcaca ttttccaact
      781 ttgaagaagc caaagcctac atcgaagagc agggggctcc aatcgtggtc aaggcagacg
      841 gcttggcact ggacaagggt gtggtcgtgg cggaaaccgt cgagcaggcg gtcgaagcgg
      901 cacgggagat gctcttggac aacaagttcg gcgattcggg tacccgcgtg gtcatcgagg
      961 agtttttggc ggggaggagt tctcgctatt tgccctggtc aatggcgacc cagaaaccca
     1021 gattatcctg cctcgtctga cctctgactt tgctcagaac atcgacgaca tcctccacaa
     1081 acgtccgacc cagctgactt ggctagatag tggcgtaacg ctgggcgtag tcgtggcatc
     1141 aaatggctac cctctggact acgaaaaagg tgtagagttg ccagcgaaga ccgagggcga
     1201 catcacgacc tactatgcag gggctcgttt tgcggaaaat agcagagcac tgctctcaaa
     1261 cggcggtcgg gtcgatatgc tggtcaccac agcagacacc gtccaagaag cccaggaaaa
     1321 aatttactcg gagctgaaaa atcaggatac gacaggcctc ttttatcgga cagatattgg
     1381 aagcaaggcc gtgaaataag ggataaagaa aaggaaagac aagtatgaac attccaattt
     1441 ccgtcatcat gggctctagt tccgactgga aaaccatgaa aaaagcagcc gaagtgctgg
     1501 acaaatttgg cgtagcctat gaaaagaaag tagtctctgc ccaccgcacg ccagacctca
     1561 tgttccgtca cgccgaagaa gctcgtggtc gtggcatcaa ggtcattatc gcaggtgcgg
     1621 gcggcgcagc tcatttgcca aggaacagga aaaaattgcg gaggcgatga cagctgacct
     1681 catctaagac aatcggaatt atcggcggcg gtcagctggg gcagatgatg gctatttctg
     1741 ctatttacat gtgccacaag gtcatcacgc tggatccagc tgcggactgc ccagcctcca
     1801 aggtcagcga ggtcatcgtc gctccctatc acgatgtggt ggccctcaaa cagttggcgg
     1861 atcggtgcga tgtcctgacc tacgagtttg aaaatgtcga tgccgatgga ctggacgcgg
     1921 tcatcaagga cggccagctc cctcaaggga cagacctcct ccgcatttcc cagaaccgca
     1981 tttttgagaa ggattttttg gcaaaaaaag ctggcgtgca agtcgccccc tacaaagttg
     2041 ttacatctag cctagaccta gaaggtctgg accttagtaa aaactacgtc ttgaaaacag
     2101 ccactggggg ctatgacgga catggtcaga aggtcattcg agaagaggcg gacttggtag
     2161 aggctagtca gttagctaac tctgccgagt gtgttttgga agagtttgtg aatttcgacc
     2221 tggaaatctc cgtccttgta tctggcaacg gctccgacta cacagtcttc cctgtgcagg
     2281 aaaatatcca ccgcaataat attctttaca aaaccatcgt gcctgcccgt atttcagacg
     2341 aacttgctga aaaagccaaa accatggccc tgcaaatcgc agacaagctt caattagcag
     2401 gcaccctctg cgttgaaatg tttgtggcag gacaggaaat tctggtcaat gaaatcgctc
     2461 ctcgcccgca caattcagga cattattcga ttgaagcctg tgatttttca cagtttgaca
     2521 cccatatccg aggcattcta ggggagcctc tgccccctat ccgcctgctc tcgccagctg
     2581 tcatgattaa cgttttggga caagatatgg aagcagctca aacctttctt caagaaaacc
     2641 ctaccgctca tccccacttt tatggtaaac tagaagcgaa gcacaatcgc aaaatgggac
     2701 atgtgacggt gttgacggat gatgttgagg tgggatttag acaagatgaa attagatagg
     2761 ctaaaggaag tagatgttag agaaatctgg aaacatgaac agtatgattt ttcggagtgg
     2821 ctgtctagga cagaaaatat tgaattgttg agtgatgaga taggattgac tcttaccgag
     2881 attgacaagg aagttttcgt tggctcatat agatgcgata tagtggctat tgatgaaaca
     2941 acgggaataa aagtgattat agaaaaccaa ttagaattaa cggatcatga tcatcttggt
     3001 aaaatcataa cctacgcatc gggtttagat gctgaggttg tcatttggat tgtaaaaaaa
     3061 gctagagaag agcataggag tgctatcgag tggcttaaca ataatacaac gaaaaatata
     3121 tcttttttcc taattgaaat ccatgcttat caaataggta gttcaatgcc tgctcctaaa
     3181 tttgttcttg tcgagaagcc gaatgatttt acaaaactag ctaaaggaaa taatcacggt
     3241 ggtgatttaa gtaaagcaca ggcggaacgt ttgtattttt ggactcaatt taataatgtt
     3301 gtggcaacta aaaataaacc gttcaacatt agaaaagcta ctacagacca ctggtatgat
     3361 gttgctattg gaacaagtga ggctcatatt tctattaccc tagtcaataa atcaaaaagt
     3421 gtagggattg aaatctatat aaacaataat aaggaattat ttgataagct atcatcacat
     3481 gctgatgaaa tccacgaaga acttggattt gaaatggaat ggaagaggtt agataataga
     3541 aaggtttcca ggattatata ttatataaaa ggtctggatt ttgagaacca tgacaattac
     3601 aacgatttaa ttgacgaagt aattaacaaa gttatcattt tgagagatgt agttaaaaag
     3661 tatatctaaa aaaataaata gatagggaaa aaggaaacac aacatgatcg aacgttacac
     3721 acgcccagag atggcggcta tttggagtga agagaacaag tacaaggctt ggttggaggt
     3781 ggaaatcctc gctgatgagg cttgggctga gttgggtgag attcccaagg aagatgtggc
     3841 tttgattcgt gagaaggcga cttttgacat cgaccgtatt ttagagattg aagaggagac
     3901 ccgtcacgat gtggtggctt tcacccgtgc ggtttcggaa agtcttggcg aggaacgcaa
     3961 gtgggtccac tatggtctga cttcgaccga tgtggtggat acggcttacg gctacctcta
     4021 caagcaggcc aacgacatca tccgtcgtga ccttgaaaac tttaccaaca ttatcgccga
     4081 caaggcgcgt gagcataagt acaccatcat gatggggcgg acccacggtg ttcatgcgga
     4141 gccaacgact ttcggtctta aattggcaac ttggtacagc gaaatgaaac gcaatatgga
     4201 gcgttttgat gtggcggcca agggtgtcga agctggtaaa atttcaggtg cagttggaaa
     4261 ctttgccaat attcctccct ttgtggaaga gtatgtttgt ggcaaattag gcattcgtcc
     4321 gcaggaaatt tctacccagg tccttcctcg tgacctccac gcaggatatt tctcagccct
     4381 agccttgatt gcaacgtcta tcgagcgtat ggcgacagaa atccgtgggc tacaaaaatc
     4441 tgaacaacgt gaagtcgaag agtatttcgc caaaggccaa aagggtagct ctgctatgcc
     4501 tcataaacgc aacccgatcg gctctgaaaa tatgaccggt ctggcacgtg tggtgcgtgg
     4561 tcacttggtg acggcttttg agaatgtggc tctctggcac gaacgcgata tttcccactc
     4621 gtcagcggag cggattatca cgccggatac gaccattctt atcaactata tgcttaatcg
     4681 ttttggcaat atcgtcaaga acttgacggt cttccctgag aatatgaaac gcaatatgga
     4741 gtcgactttt ggcttgattt acagccagcg tgtcatgctc agtttgattg aaaagggtat
     4801 gacccgtgag gaagcctatg acttggtgca accaaaaacc gcacattctt gggacaatca
     4861 agtggacttc aagccccttc ttgaagcgga tgagcgtgtg actgccaaac ttagccagga
     4921 agaaatcgat gagctcttca acccagacta ctatgctaag cgggtggatg acatctttga
     4981 acgacttgga ttataaaaaa tcaaaattct ttggattttc cagagaattt tttt
//
LOCUS       DATUCG010000145         5012 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.147, whole genome shotgun sequence.
ACCESSION   DATUCG010000145 DATUCG010000000
VERSION     DATUCG010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5012
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.147"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            11..460
                     /locus_tag="VCA20_002129"
     CDS             11..460
                     /locus_tag="VCA20_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEP1835870.1"
                     /translation="MQISNKFTIAIHALAFIHLFQDQERVTSKVLANSIQANPVIIRS
                     VLSGLKEAGIVDAKQGSGGFRLAKPLDDISLYDIFVTVDNIDKKGLFNFHENPHPDCI
                     VGGNIHAAVDDKLVQVQTAMETELKTMYMSDVLVDLEKAIEEKVKRT"
     gene            complement(570..1079)
                     /locus_tag="VCA20_002130"
     CDS             complement(570..1079)
                     /locus_tag="VCA20_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEP1835871.1"
                     /translation="MEKVKRKESLFVESVVEALWELLEDKSFEKISVSELVERAGIGR
                     VTFYRNFSNKEEVLERSFNLKLQVWLTERQIDLSDWSDAHLLLALRQFFDFWYEQQDK
                     IRLLTANHLDYLLEEVLDRYFKERLGDLTDDFHLQFIVGGFFRVLKTWVARGCKESPD
                     DIMRLMGDQ"
     gene            1220..2142
                     /locus_tag="VCA20_002131"
                     /pseudo
     CDS             1220..2142
                     /locus_tag="VCA20_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638889.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
     gene            2249..3268
                     /locus_tag="VCA20_002132"
     CDS             2249..3268
                     /locus_tag="VCA20_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEP1835872.1"
                     /translation="MKKFKKIIVWVLGIVASLGIIAYVSVTNHPQLMVGVIQSFMYKE
                     SSPNSYSPLYEPIDGLKENGQYLKSEIAYSKDYPNSFLDITYPDQNLEADRPTLFYFH
                     GGGFFGGSKNMGDPLAASQATYLIDDIVSKGYNVVNVDYALVPDHHFPTPVIQMNQAI
                     AYIKDHAEEYHLNMDNVVLMGSSAGAIMVAQYGSILANQEYAQLLNIEPSIAKEHVKA
                     LVIDDAPLDYANFSLGTKILVGNYVSETIYLSEEEINAYNPISHVNKDYPASFLLGSE
                     YRADMVSLNKALKQAGVENELVDPLAEEGLEKPHGFVANLRNDPVSAKAFERLITFID
                     DRTKE"
     gene            3490..4773
                     /locus_tag="VCA20_002133"
     CDS             3490..4773
                     /locus_tag="VCA20_002133"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEP1835873.1"
                     /translation="MSVSFEAKETNRGVLTFTIGQDAIKPELDRVFNKVKKDINLPGF
                     RKGHLPRAVFNQKFGEEALYQDVVNALLPAAYEAAVAEAGLEVVAQPKIDVVSMEKGQ
                     DWTITAEVVTKPEVKLGDYKNLAVSVEATKEVTDEEVDAKIEAARNNLAELVIKEGPA
                     AEGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFEAQLVGHAAGEEVNVEVT
                     FPEDYQAADLAGKPALFVTKIHEVKAKEVPALDDELAKDIDEEVETLDELKAKYRKEL
                     EAAKEVAFDDAVESAALELAVENAEIVELPEEMIHEEVHRAINEFLGGMQQQGISPDM
                     YFQITGTTREDLHKQYEADAEKRTKTNLVVEAVAKAEGFEATEEEINKEIEDLAATYN
                     MEVAQVRSLLSPEMLKHDIAVKKAVEVITSTATVK"
BASE COUNT         1571 a          978 c         1076 g         1387 t
ORIGIN      
        1 aaaggttgtt atgcagattt caaataaatt taccattgcc attcacgcct tggcttttat
       61 ccatctcttt caagaccagg aacgtgtcac cagcaaggtt ttggcaaaca gcattcaagc
      121 caatcccgtt atcattcgct cggttctatc tggtttgaag gaagcgggga ttgtagatgc
      181 caagcagggt agtggcggtt ttcgtttagc aaagccactt gatgacatta gcctttacga
      241 catctttgtt acggttgaca atatcgataa gaaaggcttg tttaattttc atgaaaatcc
      301 ccatcctgac tgtatcgtag ggggaaatat ccatgccgct gtggacgaca aactcgtcca
      361 agtccagaca gctatggaga cagaattgaa aaccatgtat atgtctgatg tcctggttga
      421 cctagaaaaa gccattgaag aaaaagtgaa aaggacttag attgtcatct tttcacaatg
      481 accacataca aaaaagactt agagtgactg aaaaacagtt gtctaagtct tttttgcttg
      541 gtctggtcaa gaacgttgac aagctttccc tattgatccc ccatcaatcg catgatgtca
      601 tcagggcttt ccttacaacc tctagcaacc caggttttaa gcactcggaa aaatcctccc
      661 acaatgaatt gcaggtgaaa atcatcggtc aagtccccca gtctttcttt gaaatagcga
      721 tccaagacct cctcgagcaa gtagtccaaa tggttggcag tcaataagcg aatcttatcc
      781 tgctgttcat accaaaagtc aaaaaattgc cgtaaagcta gtaagagatg ggcatctgac
      841 caatccgaca aatcaatctg ccgctcggtc aaccagactt gcaatttcaa gttgaaggac
      901 cgttcgagaa cctcttcctt attggaaaag tttcggtaaa aggtcacccg accgatgcca
      961 gctcgctcca ccaactcaga cacactaatc ttttcgaagg atttgtcttc cagcaactcc
     1021 cacaaagcct ccacgacact ctccacaaat aacgactctt tccgcttcac tttttccatg
     1081 cgaaacactt cctttcattt gtttcctttt gggcaaatag cagagctggg actagttttt
     1141 ccactgttca tctgctatac tgataaaaac gaaacacttg tttctaaaaa tataccataa
     1201 aggacagaaa aaagaaagta tgaaaaaagt tttaatcggc attgggattt taatagcttg
     1261 tttgagtatt ggtttcctgt atttagccag caagccatca gtggcttcta actacacaga
     1321 agtagttgaa acggaggagc tgttgagaaa aagtatcttg ggctaggaaa ctatgacgtt
     1381 tcctatctcg aaatcaatgc tttacaaaat ttcaaaaaat atgaactgta ctatcctaca
     1441 aatattgaaa cagaaaccag aaaatttcca gttgtgatat tttctaatgg taccggtgtt
     1501 agagcctcta agtatgcggc tgttttgaag catttggctt cctggggctt tattgtgatt
     1561 ggtactgagg aagaatattc ttggaatgga ttttcatccg aaatgagctt gcgacttgct
     1621 atcaagctaa atgataataa ggctgttgaa ggtctaaaat caaatccttt ctttagtaaa
     1681 gtcgatttag ataaggtcgg cctatcgggt cattctcaag gaggagttgg ggtctttaat
     1741 gctgttacgg aacaaaagca tgggcatatg atcaaaacca tctacgctgc aagtcctgca
     1801 aatttagaac tttctagtaa tttagagtgg gactatgacc cgagtttgat ctctgttccg
     1861 acctttcttg tctcaggtac gggtgctgga gatgaggatt tggtagtgtc tggcaagcag
     1921 ttgacacaga tttatgagac attaccagct accatcacaa agataatggc tcggagaaaa
     1981 gaagctggac atggtgatat gcttagtctt ccaaacggct atatggtagc ttggtttatg
     2041 tggcaattac aaggggaccc tgaagcagag acggcatttc aaggaaactc cccagaaatt
     2101 gttcaaaaca aactctatca agaccaaaaa cgaaacgact agaatgtatt atcgcttttc
     2161 aaaagccagt tctgtctttc tagtatttgt ccttgtttta tgttagtagc ttatttcaaa
     2221 aaatgtgtaa aaagagagga aattcttcat gaaaaaattt aaaaaaatca tcgtatgggt
     2281 ccttgggatt gtagccagtc ttggtatcat tgcttatgtg tctgttacca atcatccgca
     2341 gttgatggtc ggtgtcattc aatcgtttat gtacaaggaa agttcaccga actcttacag
     2401 tcccctatat gaacctatcg acgggttgaa agaaaatggt cagtatctga aaagtgagat
     2461 tgcctatagc aaggactacc ctaacagctt tttagatatt acctatccag accaaaatct
     2521 ggaagctgac cgtccaaccc tcttctattt ccacggtggt ggtttctttg gggggagtaa
     2581 aaatatgggg gaccctctgg cagctagcca agccacctat ctcattgacg acatcgtgtc
     2641 aaaagggtat aatgtggtca atgtggacta tgctcttgta ccagaccacc atttcccaac
     2701 gcctgtgatt cagatgaatc aggccattgc ctatataaag gaccacgctg aagaatacca
     2761 tttgaacatg gacaatgtcg tcttaatggg ttcatcagca ggtgccatca tggtagcaca
     2821 atatggtagt attttagcca atcaggagta cgctcagcta ttaaacattg aaccaagtat
     2881 cgcaaaagaa catgtcaagg ctctggtcat cgacgacgct cccttagatt atgccaattt
     2941 tagcttaggt acgaagattt tggttggaaa ttatgtttca gaaaccattt atttaagtga
     3001 ggaagaaatc aatgcctata acccgatttc ccatgtgaac aaggactacc cagcaagttt
     3061 cctcctggga agcgagtatc gagctgatat ggtgtcgctc aataaagcct tgaagcaagc
     3121 gggagttgag aatgaattgg tcgatccttt agcagaagaa gggcttgaaa agccccatgg
     3181 ttttgtggcg aatctacgaa atgacccggt gtctgctaaa gcattcgagc ggttgattac
     3241 ttttattgac gatagaacca aagaatagtc ggagagttga taagttggaa actgatgggg
     3301 atttgtccaa tcaatctgcc gctctgacaa ccatgtttgt actcagaaag tctacgccga
     3361 aaaattccaa gtgagaacaa taagtcccag ctctgccatt tcccaaaagt acgttataaa
     3421 atcgttgaaa tccctgtcaa ttgtgttata atgaagagta tgcaataaaa ttttaaggag
     3481 aatgacagaa tgtctgtatc atttgaagca aaagaaacaa accgcggcgt tttgactttc
     3541 acaatcggtc aagatgctat caaaccagaa ttggaccgcg ttttcaacaa agttaagaaa
     3601 gatatcaatc ttccaggttt ccgtaaaggt cacttgccac gtgccgtttt caaccaaaaa
     3661 ttcggtgaag aagcacttta tcaagatgtt gtaaatgcat tgttaccagc agcttatgaa
     3721 gcagcggttg cagaagctgg tcttgaagta gttgcacaac caaaaattga cgttgtgtct
     3781 atggaaaaag gtcaagactg gacaatcact gcggaagttg tgacaaaacc tgaagtaaaa
     3841 ttgggtgact acaaaaactt ggcagtttca gtagaagcta ctaaagaagt aacagatgaa
     3901 gaagttgatg caaaaatcga agctgcacgc aacaatttgg ctgaattggt aatcaaagaa
     3961 ggaccagcag ctgaaggcga tacagttgtt atcgactttg taggttcaat cgacggtgta
     4021 gaatttgacg gcggtaaagg tgagaacttc tcacttggac ttggttcagg tcaattcatc
     4081 ccaggtttcg aagcacaatt ggtaggtcac gcagctggtg aagaagtaaa cgttgaagta
     4141 actttcccag aagactatca agcagctgac cttgctggta aaccagccct ctttgtaaca
     4201 aaaatccacg aagtaaaagc aaaagaggtt ccagctttgg atgacgaatt ggcaaaagac
     4261 atcgacgaag aagtagaaac acttgatgag ttgaaagcta aataccgcaa agaattggaa
     4321 gcagcaaaag aagttgcctt tgacgatgca gttgaatcag cagctcttga attggctgta
     4381 gaaaacgctg aaatcgttga attgccagaa gaaatgatcc acgaagaagt tcaccgtgca
     4441 atcaatgagt tcttgggtgg tatgcaacaa caaggtatct caccagatat gtacttccaa
     4501 atcactggta caactcgtga agaccttcat aaacaatacg aagcagatgc tgaaaaacgt
     4561 actaagacaa acttggttgt tgaagcagta gctaaagcag aaggtttcga agcaactgaa
     4621 gaagaaatca acaaagaaat cgaagacttg gcagcaactt acaacatgga agtggcacaa
     4681 gtacgtagct tactttcacc agaaatgctt aaacacgaca tcgctgtaaa gaaagctgta
     4741 gaagtgatca caagcactgc aactgtaaaa taattatttg gaaaactcga ctctggtcgg
     4801 gttttttgtt taatcatctt tttaatgcga tgtgctgaga tgttgacacg aactctgaga
     4861 agcgaagccg gactttttgc ctaaccttgt tgtaatcgtt ttcttttgtt tgtttttgct
     4921 ttgagaaggg tcaagtaatg gtttgtaata tgtaacattt gacaaatttc aatttttcag
     4981 ttatcgttaa aaggttgttt tagtgaaatt ta
//
LOCUS       DATUCG010000146         4925 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.148, whole genome shotgun sequence.
ACCESSION   DATUCG010000146 DATUCG010000000
VERSION     DATUCG010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4925
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.148"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(67..237)
                     /locus_tag="VCA20_002134"
     CDS             complement(67..237)
                     /locus_tag="VCA20_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003002781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEP1835874.1"
                     /translation="MAKGFGKGFLTGVLSTVALAAGAVFTVHKTIIEPEEKKEAFIEE
                     NRKKAARRRVAH"
     gene            306..1190
                     /gene="miaA"
                     /locus_tag="VCA20_002135"
     CDS             306..1190
                     /gene="miaA"
                     /locus_tag="VCA20_002135"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEP1835875.1"
                     /translation="MKTKVIVVIGPTAVGKTALGIDLAQCYHGEIISGDSQQVYRKLD
                     IGTAKASPEEQAAAVHHLIDVRDVTEGYSAYEFVAEAKALIADLTSRGKLPVIVGGTG
                     LYIQSLLEGYHLGGLVDQEQVLAYRAELDCLSDEDLETMAEQAGLMVEGNSRRRIIRG
                     LELKKFGENLENTESGYEPLYICLTDDRRVLYDRINQRVDKMMAAGLLDEVSWLYQEH
                     PEAQAAMGIGYKEFFPYFAGELSLEEAIDKVKQNSRRFAKRQLTWFRNRMQVPFYSVG
                     EPDCKSQIFTAVEEFLND"
     gene            1183..2433
                     /gene="hflX"
                     /locus_tag="VCA20_002136"
     CDS             1183..2433
                     /gene="hflX"
                     /locus_tag="VCA20_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEP1835876.1"
                     /translation="MIETQKEQERALLVGVELQQTDNFDMSMEELASLAKTAGALVKG
                     VYTQKREKYDSKTFVGSGKLEEIAQMVEADEIDTVIVNNRLTPRQNVNLEEILGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYMLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSIRNQIHDIERQLKTVEKNRATVRERRLQSGVFKIGLIGYTNAGKSTIMNAM
                     TDKRQYEADELFATLDATTKQINLADKFNVTLTDTVGFIQYLPTELISAFKSTLEESM
                     NVDLLLHVIDASDPNHSEQEQVVLDILKDLDMLDIPRLALYNKLDKTDDSFTPSQFPH
                     VMLSAKDENAKGHIQMMVLAKIKTMFERFEVRVPLAEAYKLHDLATLALIENRTYEED
                     VEVVSGYIASSNKWKLEEFYDGLS"
     gene            2417..3046
                     /locus_tag="VCA20_002137"
     CDS             2417..3046
                     /locus_tag="VCA20_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEP1835877.1"
                     /translation="MTDYLDLAMKYGGFTSLDKVYLAKKLADLTDQQKLDFITPPPSV
                     INAYFAEIYQKQGAEEATDYYLTLSQQLCLFPKEPSFAEDKPFVRLNLSGKSFGFAYV
                     SADGLAQVFSEGVEEVTGSLLFEIAHVFPHYVVFVEEGKIFMKVNPFEEAELEEVETD
                     YLLTQVATAKGLVKISGFNQEEVLEVADGYAGQYYYVWSGRSAILYIKH"
     gene            3057..3986
                     /locus_tag="VCA20_002138"
     CDS             3057..3986
                     /locus_tag="VCA20_002138"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEP1835878.1"
                     /translation="MQIQFLGTGAGQPSKARNVSSLALKLLDEINQVWLFDCGEGTQN
                     QILETTIRPRKVAKIFITHLHGDHIFGLPGFLSSRSFQSSEEQTDIDIYGPVGIRSFV
                     LASLKVSGTRLPYRIHFHEFDVDTVGQVLETDKFTVFAEKLDHTIPCVGYRVIQKDLE
                     GTLDAEALRAAGVPFGPLFGKVKNGQNVMLEDGTEIIATDYISPPRPGKVVTILGDTR
                     KCHASVRLAVNADVLVHEATYGKGDEKIARKHGHSTNMEAAQVAKDAGVKQLLLNHIS
                     PRFLSKDISQLRKDASTIFEQVHIVKDLEEIEL"
     gene            3983..4738
                     /locus_tag="VCA20_002139"
     CDS             3983..4738
                     /locus_tag="VCA20_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEP1835879.1"
                     /translation="MRTILITGASGGLVQEMVPLLKDDFLILLGRDVAKIEQLYAYHE
                     KKAVFDVDIRDEIALTSFFDELDSQFSQIDILVNNAGYAIYDDFENFSSQQVQAMFDI
                     NTFALMTMCRLVGKRMKTRRSGQIINIISMSGLIASAKSSVYSATKFAAMGFSNTIRL
                     ELAQYGVTVTTVNPGPIATGFFDQADPDGSYQESVKAFLLQPDYVAKKIVSAMGTKKR
                     DINLPWSLAAAHKLYTLFPRIADYLASTVFNLK"
BASE COUNT         1364 a         1028 c         1202 g         1331 t
ORIGIN      
        1 gacgggaata gtttctttag ctgcttaaat gcttaactta atgacattgg gacatttgcc
       61 agctttttaa tgtgccacac ggcgacgtgc agctttttta cgattttcct caatgaaggc
      121 ttctttcttt tcttctggtt caatgattgt tttgtgaaca gtgaagactg cgcctgctgc
      181 aagagcaact gtagaaagaa cacctgttaa gaaaccttta ccaaaacctt tagccatagt
      241 tatttctcct ttacttgtgt tataatagat tttagcgaaa aaacaaaata ttttcaagga
      301 aaaagatgaa aactaaagtt attgtggtca ttggaccaac agcggtagga aaaactgccc
      361 ttggcataga cttggcccag tgctaccatg gagaaattat cagcggtgat agccagcaag
      421 tctatcgcaa acttgatatt ggtacggcga aagctagtcc tgaagagcag gctgctgcgg
      481 ttcatcactt gattgatgtc cgagatgtga ccgagggcta ttcggcttat gaatttgtag
      541 cagaagccaa ggccttaatt gcagacctta ctagtcgtgg aaagttgccc gttatcgttg
      601 gaggaactgg gctttacatt cagagcttgc ttgaaggcta ccatctgggt gggctggttg
      661 accaggagca agttcttgct tatcgggcgg aactcgactg cttgtctgac gaggatttgg
      721 aaacaatggc agagcaggca ggtttgatgg tcgagggaaa cagccgacgg agaatcattc
      781 gtggactgga attgaaaaaa tttggcgaaa acttagaaaa tacagagtca ggatatgagc
      841 cactttacat ctgtttgacg gatgacagac gggttcttta cgaccgcatc aatcagcgcg
      901 tggataagat gatggcggcg ggcttactgg atgaagttag ctggctctac caagaacacc
      961 cagaagctca agccgctatg ggaattggct acaaggagtt tttcccttat tttgcaggtg
     1021 agctttcgct agaagaagca attgataaag tcaagcaaaa tagccgccgc tttgccaaac
     1081 gccaattgac ttggttcaga aatcgcatgc aggttccctt ctattcagta ggagagccag
     1141 attgcaagtc gcagattttc accgccgtgg aggaattttt aaatgattga aacgcagaaa
     1201 gaacaagaac gcgctctcct ggttggagtg gaattacagc agacagataa ttttgacatg
     1261 tccatggagg agttggcaag tcttgctaag acggctggcg ctctggtcaa gggagtctac
     1321 actcaaaaac gagaaaaata cgacagcaag acctttgtcg gctcagggaa actagaagaa
     1381 attgcccaga tggtcgaagc tgatgagatt gataccgtca ttgtcaataa ccgactgaca
     1441 ccccgccaga atgtcaatct ggaagagatt ttaggtgtca aagtcatcga ccgtatgcag
     1501 ctgattttgg atatttttgc catgcgggca cggagtcacg aaggaaaact acaagtccac
     1561 ctggcccagc tcaagtatat gttgcctcgt ttggttggac agggtatcat gctcagccgt
     1621 caggcaggtg gtatcggctc tcgtggacca ggtgaaagcc agttggagct taaccgccgg
     1681 agcattcgca accagattca tgacattgag cgccaactca aaacagtgga gaaaaaccga
     1741 gcgactgtcc gtgaacgccg cctgcaatca ggcgtcttca aaatcggctt gattggctat
     1801 accaatgctg gtaagtccac tatcatgaat gccatgaccg acaagcgtca gtacgaggct
     1861 gatgaacttt ttgccacact agatgccaca accaagcaga ttaacttggc ggacaagttc
     1921 aacgtgaccc tgactgacac ggttggtttc attcagtacc tgccgaccga gttgatttct
     1981 gccttcaagt ccaccttgga ggagtccatg aatgtggacc tcttgctcca tgtcatcgac
     2041 gcctctgacc ccaatcacag tgagcaggaa caggtggtct tggacattct aaaagacctg
     2101 gatatgctgg acattccacg tctagctctc tacaacaagc tggacaagac cgatgacagc
     2161 tttaccccca gtcagtttcc tcatgtcatg ctgtccgcta aggatgaaaa tgccaaaggc
     2221 catattcaga tgatggtctt ggccaagatt aagaccatgt ttgagcgatt tgaggtcagg
     2281 gtgccactgg ccgaggctta caagttgcat gatttggcaa ccctggcact gattgaaaat
     2341 cggacctacg aagaggatgt ggaagtggta tcaggctata tcgcttccag caataaatgg
     2401 aagttggaag aattttatga cggattatct tgatttggca atgaaatacg gtggctttac
     2461 cagtcttgac aaggtctatc tggctaagaa attagctgat ttgactgacc aacaaaaact
     2521 ggattttatt acgccaccgc cttcggttat caatgcctat tttgcggaaa tctaccagaa
     2581 acaaggggca gaagaggcga cggactatta tctcactcta tctcagcagc tctgcctttt
     2641 tccaaaagaa cccagctttg ctgaagacaa gccctttgtc cgcctgaatt tatctggtaa
     2701 gtctttcgga tttgcctatg tgtcggctga cggtctggcc caggtttttt cggaaggtgt
     2761 agaggaagtg acaggaagtt tgctctttga aatcgcccac gtctttcccc actatgtcgt
     2821 ctttgtggag gagggcaaga ttttcatgaa agtcaatccc tttgaggaag ccgagttaga
     2881 ggaagttgaa acagactacc ttttgaccca agtggcgaca gcgaaaggtt tggtcaagat
     2941 ttcaggcttt aatcaggaag aagtgctgga agtggcggac ggctatgctg gtcagtatta
     3001 ctatgtctgg tcgggtcgct ctgcaatttt atatataaaa cattagaaag taaacaatgc
     3061 aaattcaatt tttaggtacg ggggctggtc agccctccaa ggctcgaaat gtatccagtt
     3121 tagccttgaa actcttggat gaaatcaatc aggtttggct atttgactgt ggtgaaggaa
     3181 ctcaaaatca aattttagaa acgactatcc gcccacgtaa ggtcgcaaaa atctttatca
     3241 ctcacctgca cggagaccat atttttggac tgccaggttt tttatccagt cgttctttcc
     3301 agtcaagtga ggaacagacg gatattgata tttacggacc tgttggcatc cgttcttttg
     3361 ttttggcaag tcttaaagta tcagggacac gcctacctta tcgcattcat tttcatgagt
     3421 ttgatgttga tacggtaggt caggtcctag aaacggacaa attcacggta ttcgctgaaa
     3481 agctggacca taccattcct tgtgtcggct atcgtgtcat ccagaaagat ttggaaggga
     3541 cattggatgc agaggctctg cgcgcggcag gtgtaccatt tggtccccta ttcggtaaag
     3601 tcaaaaatgg tcaaaacgtg atgctagaag atgggactga aattatcgcg actgactata
     3661 tttctcctcc tcggcctggt aaggtagtga ccattcttgg tgatacgcgc aagtgtcatg
     3721 ccagtgtccg tctagcggtt aatgctgatg tgctggtgca tgaggcgact tacggaaaag
     3781 gcgatgagaa gattgctcgc aagcatggtc attcgaccaa tatggaagcc gctcaggttg
     3841 ccaaggatgc tggtgtcaag cagctcttac tcaatcatat cagtccacga tttttatcta
     3901 aggatattag tcaattacgg aaagatgcaa gtactatttt cgagcaggtt catattgtca
     3961 aggatttaga ggaaattgag ctatgagaac cattctaatc acaggtgcct ctggtggttt
     4021 ggtacaggaa atggtgccat tgctgaaaga tgattttttg attttactag gtcgtgatgt
     4081 ggcaaaaatt gagcagctct atgcttacca tgaaaaaaag gctgtctttg atgtagacat
     4141 ccgagatgaa atagctctga cgtcattttt cgatgaactg gatagtcagt ttagtcagat
     4201 tgatattcta gtcaataatg ctggctatgc catttatgat gattttgaga atttctccag
     4261 ccagcaggtg caagctatgt ttgacatcaa tacttttgct ctgatgacca tgtgccgttt
     4321 agtaggtaaa cgaatgaaga caagacggag cgggcagatt atcaatatca tttccatgtc
     4381 aggcttgatt gcttctgcca agtcttcggt ctattcggca accaagtttg cagctatggg
     4441 tttttcaaat accattcgct tggaattagc ccagtatggt gtgacagtta caactgttaa
     4501 cccaggacct attgcgactg gctttttcga ccaagcagat ccagacggaa gctatcaaga
     4561 gagcgtcaag gctttcttgc tgcaaccaga ctatgttgcc aagaagattg tttcagctat
     4621 ggggacgaag aaacgagaca tcaatctgcc ttggtcacta gcagctgctc ataaactcta
     4681 tactctcttt ccaagaatag cagactactt agccagtaca gtatttaatt taaaataagc
     4741 atagaaaaac gccgaaataa aactcggcgt tttcgtgtag actagctacg ctcttttcta
     4801 cgtaaggcga tactgcctag aagtaggcta cctaatgcga tagcgatata tagtcaactg
     4861 aaacaagagc aggactaaag tacctcaaga aacgtatcgc aattcggcaa tactcttctg
     4921 aggtg
//
LOCUS       DATUCG010000147         4894 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.149, whole genome shotgun sequence.
ACCESSION   DATUCG010000147 DATUCG010000000
VERSION     DATUCG010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4894
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.149"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            29..1577
                     /locus_tag="VCA20_002140"
     rRNA            29..1577
                     /locus_tag="VCA20_002140"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1631..1703
                     /locus_tag="VCA20_002141"
     tRNA            1631..1703
                     /locus_tag="VCA20_002141"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1664..1666,aa:Ala,seq:tgc)
     gene            1869..4772
                     /locus_tag="VCA20_002142"
     rRNA            1869..4772
                     /locus_tag="VCA20_002142"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         1356 a         1004 c         1433 g         1101 t
ORIGIN      
        1 agcaatagct tgagtttgaa tcaaaacttt ttatgagagt ttgatcctgg ctcaggacga
       61 acgctggcgg cgtgcctaat acatgcaagt agaacgctga agtctggtgc ttgcactaga
      121 cggatgagtt gcgaacgggt gagtaacgcg taggtaacct gcctcatagc gggggataac
      181 tattggaaac gatagctaat accgcataac agtatttacc gcatggtaga tgcttgaaag
      241 gagcaactgc ttcactatga gatggacctg cgttgtatta gctagttggt gaggtaacgg
      301 cttaccaagg cttcgataca tagccgacct gagagggtga tcggccacac tgggactgag
      361 acacggccca gactcctacg ggaggcagca gtagggaatc ttcggcaatg ggggcaaccc
      421 tgaccgagca acgccgcgtg agtgaagaag gttttcggat cgtaaagctc tgttgtaaga
      481 gaagaactgt gagaagagtg gaaagtttct cacttgacgg tatcttacca gaaagggacg
      541 gctaactacg tgccagcagc cgcggtaata cgtaggtccc gagcgttgtc cggatttatt
      601 gggcgtaaag cgagcgcagg cggtttgata agtctgaagt aaaaggctgt ggcttaacca
      661 tagtacgctt tggaaactgt caaacttgag tgcagaaggg gagagtggaa ttccatgtgt
      721 agcggtgaaa tgcgtagata tatggaggaa caccggtggc gaaagcggct ctctggtctg
      781 caactgacgc tgaggctcga aagcgtgggg agcgaacagg attagatacc ctggtagtcc
      841 acgccgtaaa cgatgagtgc taggtgttgg gtcctttccg ggactcagtg ccgcagctaa
      901 cgcattaagc actccgcctg gggagtacga ccgcaaggtt gaaactcaaa ggaattgacg
      961 ggggcccgca caagcggtgg agcatgtggt ttaattcgaa gcaacgcgaa gaaccttacc
     1021 aggtcttgac atcccagtga ccgtcctaga gataggattt ttcttcggaa cactggtgac
     1081 aggtggtgca tggttgtcgt cagctcgtgt cgtgagatgt tgggttaagt cccgcaacga
     1141 gcgcaacccc tattgttagt tgccatcatt cagttgggca ctctagcgag actgccggta
     1201 ataaaccgga ggaaggtggg gatgacgtca aatcatcatg ccccttatga cctgggctac
     1261 acacgtgcta caatggctgg tacaacgagt cgcaagtcgg tgacggcaag ctaatctctt
     1321 aaagccagtc tcagttcgga ttgtaggctg caactcgcct acatgaagtc ggaatcgcta
     1381 gtaatcgcgg atcagcacgc cgcggtgaat acgttcccgg gccttgtaca caccgcccgt
     1441 cacaccacga gagtttgtaa cacccgaagt cggtgaggta accttttagg agccagccgc
     1501 ctaaggtggg atagatgatt ggggtgaagt cgtaacaagg tagccgtatc ggaaggtgcg
     1561 gctggatcac ctcctttcta aggaaatgga aacctgtacg tcagtcttct ttaattttga
     1621 gaggtcttgt ggggccttag ctcagctggg agagcgcctg ctttgcacgc aggaggtcag
     1681 cggttcgatc ccgctaggct ccattaacaa cggaagttgt taagattttg tccattgaaa
     1741 attgaatatc tatcaaacat tcctaaacgt atgtaaaagt acgtatagaa atagtaacaa
     1801 gaaaataaac cgaaaacgct gtgaatattt aatgagtttt ctaatttttg aaaaaattaa
     1861 gttaataagg ttaagttaat aagggcgcac ggtggatgcc ttggcactag aagccgatga
     1921 aggacgtgac taacgacgaa atgccttggg gagctgtaag taagcaatga tccagggatg
     1981 tccgaatggg ggaacccggc aggtaatgcc tgtcactcac tactgttaag gtagtgaaga
     2041 ggaagacgca gtgaactgaa acatctaagt agctgcagga agagaaagca aaagcgattg
     2101 ccttagtagc ggcgagcgaa acggcaggag ggcaaaccga ggagtttact cctcggggtt
     2161 gtaggactgc aatgtggact taaagagtat agaagaacta cctgggaagg taggccaaag
     2221 agagtaatag cctcgtattt gaaatattct ttatacctag cagtatcctg agtacggcga
     2281 gacacgcgaa atctcgtcgg aatctgggag gaccatctcc caaccctaaa tactctctag
     2341 tgaccgatag tgaaccagta ccgtgaggga aaggtgaaaa gcaccccgga aggggagtga
     2401 aatagaacct gaaaccgtgt gcctacaaca agttcgagcc cgttaatggg tgagagcgtg
     2461 ccttttgtag aatgaaccgg cgagttacga tatgatgcga ggttaagttg aagagacgga
     2521 gccgtaggga aaccgagtct taatagggcg gattagtatt atgtcgtaga cccgaaacca
     2581 tgtgacctac ccatgagcag gttgaaggtg cggtaagacg cactggagga ccgaaccagg
     2641 gcacgttgaa aagtgcttgg atgacttgtg ggtagcggag aaattccaaa cgaacttgga
     2701 gatagctggt tctctccgaa atagctttag ggctagcgtc gacatttgag aatcttggag
     2761 gtagagcact gtttggatga gggggccatc tcggtttact gatttcagat aaactccgaa
     2821 tgccaatgat ttatggtcgg cagtcagact gcgagtgcta agatccgtag tcgaaaggga
     2881 aacagcccag accaccagct aaggtcccaa aataattgtt aagtggaaaa ggatgtgggg
     2941 ttgcacagac aactaggatg ttagcttaga agcagctatt cattcaaaga gtgcgtaata
     3001 gctcactagt cgagtgaccc tgcgccgaaa atgtaccggg gctaaaacaa tttaccgaag
     3061 ctgtggataa cacattagtg ttatggtagg agagcgttct atgtgtgaag aaggtatacc
     3121 gtgaggagtg ctggaacgca tagaagtgag aatgccggta tgagtagcga aagatgggtg
     3181 agaatcccat ccaccgtaag actaaggttt ccaggggaag gctcgtccgc cctgggttag
     3241 tcgggaccta aggagagacc gaaaggtgta tccgatggac aacaggttga tattcctgta
     3301 ctagagtatg aagtgatgga gggacgcagt aggctaacta aagcgggcga ttggaagtgc
     3361 ccgtctaagc agtgaggtgt gatatgagtc aaatgcttgt atctataaca ttgagctgtg
     3421 atggggagcg aagttaagta gcgaagttag tgacgtcaca ctgccgagaa aaacttctag
     3481 cgatgtatca tactctaccc gtaccgcaaa ccgacacagg tagtcgaggc gagtagcctc
     3541 aggtgagcga gagaactctc gttaaggaac tcggcaaaat gaccccgtaa cttcgggaga
     3601 aggggtgctg actgaaggtc agccgcagtg aataggccca agcaactgtt tatcaaaaac
     3661 acagctctct gctaaatcgt aagatgatgt atagggggtg acgcctgccc ggtgctggaa
     3721 ggttaagagg agggtttagc gcaagcgaag atctgaattg aagccccagt aaacggcggc
     3781 cgtaactata acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg
     3841 aaaggcgtaa tgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt
     3901 gaagatgcag gttacccgcg acaggacgga aagaccccat ggagctttac tgcagtttga
     3961 tattgagtat ctgtaccaca tgtacaggat aggtaggagc ctacgaagtc gggacgccag
     4021 tttcgacgga ggcgctgttg ggatactacc cttgtgttat ggctactcta acccggatag
     4081 gttatcccta tcggagacag tgtctgacgg gcagtttgac tggggcggtc gcctcctaaa
     4141 aggtaacgga ggcgcccaaa ggttccctca gaatggttgg aaatcattcg cagagtgtaa
     4201 aggtataagg gagcttgact gcgagagcta caactcgagc agggacgaaa gtcgggctta
     4261 gtgatccggt ggttccgtat ggaagggcca tcgctcaacg gataaaagct accctgggga
     4321 taacaggctt atctccccca agagttcaca tcgacgggga ggtttggcac ctcgatgtcg
     4381 gctcgtcgca tcctggggct gtagtcggtc ccaagggttg ggctgttcgc ccattaaagc
     4441 ggcacgcgag ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcgggcgt
     4501 aggaaatttg agaggatctg ctcctagtac gagaggacca gagtggactt accgctggtg
     4561 taccagttgt cttgccaaag gcatcgctgg gtagctatgt agggacggga taaacgctga
     4621 aagcatctaa gtgtgaaacc cacctcaaga tgagatttcc cataacttta tgttagtaag
     4681 agccctgaga gatgatcagg tagataggtt ggaagtggaa gtgtggcgac acatgtagcg
     4741 gaccaatact aatcgctcga ggacttatcc aaagaaataa actagagtca atattgacag
     4801 cgttggtaat tcttgttaga atatagatat tcaattttga gttgacaaga ctcagtagtt
     4861 aagtgacgat agcctaggag atacacctgt accc
//
LOCUS       DATUCG010000148         4858 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.150, whole genome shotgun sequence.
ACCESSION   DATUCG010000148 DATUCG010000000
VERSION     DATUCG010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4858
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.150"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(<1..268)
                     /locus_tag="VCA20_002143"
     CDS             complement(<1..268)
                     /locus_tag="VCA20_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HEP1835880.1"
                     /translation="MLDQIKAHLLDSINYIVSSANQFVLHPEKDFSRKSQLTMETMIQ
                     AILTMGGNTLAKELLDLDLPVTQSAFVQRRYQIKHQAFKTLFRDI"
     gene            457..822
                     /locus_tag="VCA20_002144"
     CDS             457..822
                     /locus_tag="VCA20_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEP1835881.1"
                     /translation="MTVKINTKDGQIELTDDVIATVVGGATTEIFGVVGMASKSAIKD
                     NFQALLRKENYSKGVVIKTLEDGRIAVDVYTVMSYGVKISEVSRNIQERVKFNLENQL
                     GISVDAVNVFIQNIKVVGE"
     gene            825..2489
                     /locus_tag="VCA20_002145"
     CDS             825..2489
                     /locus_tag="VCA20_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004193336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEP1835882.1"
                     /translation="MSKITTSLFQEMVQAASTRLNKQAEYVNSLNVFPVPDGDTGTNM
                     GMTITNGAKEVADNSASTVGEVAQILSKGLLMGARGNSGVITSQLFRGFGQSVKGKVE
                     LDGKDLAQAFQHGVEVAYKAVMKPVEGTILTVSRGAATAALKKAEETDDAVEVMRATL
                     DGANRALKKTPEMLPVLKEVGVVDSGGQGLVYIYEGFLSALTGEYIASEDFEATPAVM
                     SEMINAEHHKSVAGHVATEDITFGYCTEIMVALRQGPTYVKEFDYEEFRNYLNDLGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQAGLQYGSLVKVKVDNMREQHEAQVEKEETFQKPT
                     EQKEYAIIAVAAGEGLTEIFKSQGVDYVISGGQTMNPSTEDFVNAIELVNARNVILLP
                     NNKNILMAAQTAAEVAEVAVKVVETKTLPQGFTSLLAFDPSRDLESNFEAMTASLAEV
                     KSGSVTTAVRDTTIDGLEIHENDNLGMVDGKIVVSNPDMMETLVATFEKMLDEDSEIV
                     TIYIGEDGDEELAQTLAEQLEEQFEDVEVEIHQGNQPVYPYLFSVE"
     gene            2554..3030
                     /gene="tsaE"
                     /locus_tag="VCA20_002146"
     CDS             2554..3030
                     /gene="tsaE"
                     /locus_tag="VCA20_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEP1835883.1"
                     /translation="MTNYWGYGIIKDMYSQNENELIAIGERIGKACKPNQVLVLSGDL
                     GAGKTTLTKGLAKGLKIEQMIKSPTYTIVREYEGTMPLYHLDVYRIGDDPDSIDLDDF
                     LYGGGLTVIEWGELLDVSLFDDYLLIRIEKEGDGRRLTVESHGAQSSDLAKEFQNV"
     gene            3023..3550
                     /locus_tag="VCA20_002147"
     CDS             3023..3550
                     /locus_tag="VCA20_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEP1835884.1"
                     /translation="MSEKEVYFSEAEPADAAAFIDFMNQVARETDYLVMDETGFRFSV
                     DEIETIFEAGIENPRELCLLAKVGSEVVGAISVKSSKQFRISHIGNIFIAVKKAYWGH
                     GLGTILLEEVLHWAEEMDVLKRLELTVQVRNQAAVHLYQKLGFNIEGTQIRGARTDEG
                     EWLDLYYMGKLIGEV"
     gene            3547..4740
                     /locus_tag="VCA20_002148"
     CDS             3547..4740
                     /locus_tag="VCA20_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEP1835885.1"
                     /translation="MTIGKKIFLMSLAIVGLTLGAGLIYGASLLNFSTNAISKTFKQL
                     DGEEKVTPIDATEPLTILLMGVDMDQATRGGDWEGGRSDSMILVTVNPKTKETNMMSL
                     TRDIMVEIAEANGESSGTVEKLNHSYSYGQAPMAIATIEKMMDINIDHYIEINMDGLV
                     ELVDAVGGIEVNNTLGFPISISEHEPAYTSIVQPGKQLVNGDQALVYARMRYDDPEGD
                     IGRQRRQREVITAIIKKLLQLDGFTQYKKILTAISNNMRTNIEISPATIPNLLGYKDS
                     VSKLNSYQLRGVDQMVDAIYYQLPTSTHLLEMQNVLKKSIGLEEKTDLITNVKVYEGQ
                     MGLPSPINVYDVYTNLLLLEASPWAESLDPEIGSPSATTTVTTEEQVTDFSTEPVLGD
                     ENGSQ"
BASE COUNT         1462 a          827 c         1170 g         1399 t
ORIGIN      
        1 taatatccct aaaaagtgtt ttaaaagctt ggtgtttaat ctgataccgt cgttgaacaa
       61 aggcagattg agtgacaggc aaatctaaat caagtagctc tttggctaag gtattacctc
      121 ccatggtcag tatagcttga atcatggttt ccatagttag ctgacttttc cgactaaaat
      181 ctttttcagg atgaagcaca aattgattgg cactagaaac gatgtagtta atactatcaa
      241 gtaaatgagc cttaatctga tctagcatga cttttcccct tttattttta gtgtaacata
      301 aaaataaccc accacaaaat gtgataggtt agttaactta atgacattgc ctttgggaac
      361 tctttttttg taaaatccga tgtaacaagc ttgtttttta cagaaagcag aaaatgtggt
      421 aaaatggagt gataaaacta tttgaggtaa cgaattatga ctgttaaaat caatactaaa
      481 gacggccaaa tcgaattgac tgatgacgtg attgccacag ttgttggcgg tgctacgact
      541 gagattttcg gtgttgttgg gatggctagt aagtctgcga tcaaggataa ttttcaagca
      601 cttttgagaa aagaaaatta ttctaaaggt gttgtgatta agaccttgga agatggtcgc
      661 attgcagtcg atgtttatac tgtgatgagc tacggtgtaa aaatcagtga agtatctcgc
      721 aacatccaag aacgcgttaa gttcaacttg gaaaatcaac taggtatctc tgtagatgct
      781 gtcaatgtct ttatccaaaa tatcaaagtc gtaggagaat aattgtgtct aaaattacaa
      841 ctagtttatt tcaagaaatg gtgcaggcag catcaactcg tctgaataaa caagccgaat
      901 atgtgaactc actcaatgtt ttcccagttc cagatgggga tactgggacc aacatgggga
      961 tgactattac caatggtgct aaagaagtag cagataattc tgcttctact gttggagaag
     1021 ttgcgcaaat tctttctaag ggcttgttga tgggagctcg tggaaactca ggtgttatta
     1081 cgtcgcaatt gttccgcggt tttggtcaat ctgttaaagg caaggttgaa ttggacggta
     1141 aggatttggc gcaagccttt caacatggtg tagaagtggc ttataaagcc gttatgaagc
     1201 cagttgaagg tacaattctt actgtttcgc gtggggcagc cactgccgct ttgaaaaagg
     1261 cagaagagac tgatgatgcg gttgaggtca tgcgtgccac tcttgacggt gctaatcgtg
     1321 ctcttaagaa aacgccagag atgcttccag tattgaagga agttggtgtt gtcgattcag
     1381 gtggacaagg tcttgtttac atttatgaag gcttcttgtc agctttgacg ggtgagtata
     1441 ttgcttcgga agattttgag gcgacacctg ctgttatgtc tgaaatgatc aatgctgagc
     1501 accacaagtc agttgcaggt catgtggcga ctgaagatat tacctttggt tactgtacag
     1561 aaatcatggt agctcttcgc caaggtccta cttatgtgaa ggaatttgat tatgaagaat
     1621 tccgtaacta cctcaacgac cttggggatt ccctcctagt tgttaacgac gatgaaatcg
     1681 tcaaagtcca tgtccataca gaagatccag gtttggtcat gcaagcgggt cttcaatatg
     1741 gtagcttggt caaggtaaaa gtggacaata tgcgtgagca acatgaagct caggtcgaaa
     1801 aagaagaaac tttccaaaaa ccgactgaac aaaaagagta tgctattatt gcggttgctg
     1861 ctggtgaagg tttgacagaa atcttcaaat cacaaggcgt tgactatgtg atttcaggtg
     1921 gtcagaccat gaacccatca acagaagatt ttgtcaatgc tattgaattg gtcaatgccc
     1981 gcaatgtcat cttgttgcca aacaacaaaa atatcttgat ggctgctcag acggctgctg
     2041 aggttgcaga agttgcggtt aaggttgtgg aaactaagac cctaccacaa ggttttacta
     2101 gcttgcttgc ttttgatcca agtcgtgatt tggaaagcaa ctttgaagcg atgacggcat
     2161 ctctcgcaga ggtgaaaagt ggtagcgtga caactgcggt ccgcgataca accattgacg
     2221 gtcttgaaat ccatgagaat gacaaccttg gtatggttga tggcaagatt gtggtttcta
     2281 atccagatat gatggaaaca cttgtcgcta catttgagaa aatgctggat gaagatagtg
     2341 aaatcgtaac aatctatatc ggtgaagatg gtgatgagga actcgctcaa actcttgctg
     2401 aacagctaga agaacaattt gaagatgttg aggtcgaaat ccatcaagga aatcaaccag
     2461 tctatccata tcttttcagc gtagaataaa caaaatcgca ggctctggct tgcgattttt
     2521 tctgtcgact tgtctacaat ttccagtaga cctatgacaa actactgggg ctatggtata
     2581 attaaagaca tgtatagtca gaatgaaaat gaattgattg ccattggtga gagaattgga
     2641 aaagcctgta agccaaatca agttctagta ttatcagggg atttgggtgc tgggaaaaca
     2701 actctgacca agggcttggc caaggggtta aaaattgaac agatgattaa gagccctact
     2761 tatacgatcg ttcgagagta tgaggggacc atgccactct atcacttaga tgtttatcga
     2821 attggagatg accctgactc gattgatttg gatgattttc tctatggagg aggtctaacg
     2881 gttatcgagt ggggagaatt actggatgtc agtctatttg atgactattt gctcattcgt
     2941 atagagaaag agggagatgg tcgacgattg acagtcgaat ctcatggagc tcaaagtagc
     3001 gatttagcga aggagttcca aaatgtctga aaaagaagtg tattttagtg aagctgaacc
     3061 agctgatgca gcagctttta ttgattttat gaatcaagtt gctagagaaa cggattatct
     3121 ggtcatggat gagactggtt ttcgatttag tgttgatgaa atagagacca tcttcgaagc
     3181 agggatcgaa aatcctcgtg aattgtgcct gttggctaag gttggttcgg aagtcgtcgg
     3241 cgcaatttct gttaagtcct ctaagcagtt tcgcattagc catatcggaa atatttttat
     3301 tgcagtaaaa aaagcctatt ggggtcacgg tctagggacg attttactgg aggaagtatt
     3361 gcattgggca gaggaaatgg atgtattgaa gcgtttggaa ttaacggttc aggttcgcaa
     3421 tcaggcggca gtacatttat atcaaaaact aggatttaac attgaaggaa cccagatacg
     3481 tggggctaga acagatgaag gagaatggct tgatctttat tatatgggca agttaattgg
     3541 tgaagtatga caattggaaa aaaaatattc ttaatgtctt tggctattgt tggtttaaca
     3601 ctaggtgccg gcttaatata tggggccagc ttgttaaatt tttctacaaa tgctatttcg
     3661 aaaacattta agcagctaga tggagaagaa aaagtcactc cgattgatgc gacagagccc
     3721 ctaactatcc tactgatggg ggtggatatg gaccaagcga cccgtggagg agattgggag
     3781 ggggggcgta gtgattcgat gattttggta actgttaatc ccaagactaa ggaaaccaat
     3841 atgatgagct tgactcgtga tattatggtt gagattgcag aagcgaatgg ggagtcttca
     3901 ggaacggttg aaaaactaaa tcattcttat agctacggac aggcaccgat ggccattgcc
     3961 acgatcgaaa agatgatgga tattaacatt gaccactata tcgaaataaa tatggatggt
     4021 ctggttgagc ttgttgatgc ggttggaggt attgaggtaa acaatactct tggcttccct
     4081 atatccattt cggaacatga gcctgcctac acctctattg ttcagccggg gaaacagttg
     4141 gtaaatgggg atcaggcgct ggtttatgct cggatgcgct atgatgatcc tgagggggat
     4201 attggtcgtc agagacgtca acgtgaagtt ataacggcta ttataaaaaa actccttcag
     4261 ttagacggtt ttacccagta taagaagatt ctgactgcca tttctaataa tatgcgaacc
     4321 aacattgaga ttagtcctgc gacaattcca aatctcttgg ggtacaagga ctctgtctcc
     4381 aagttgaatt cctatcagtt gcgcggtgtg gatcaaatgg ttgatgcaat ctattatcaa
     4441 ttaccaacca gtacacatct attggaaatg caaaatgttc tgaaaaaatc tatcggctta
     4501 gaagaaaaga ccgacttaat cacaaacgtg aaggtctacg aaggacagat gggattgcca
     4561 agtccaataa acgtttatga tgtatataca aatctcttgc tgttagaagc aagtccatgg
     4621 gcagaaagct tggatccaga aatagggagt ccatcggcta ctacgactgt gacaacagaa
     4681 gagcaggtaa ccgatttttc aacagagcca gtactcggtg atgaaaacgg gtcacaataa
     4741 tgtagtcatt cagtttttct tttattactt tgatgagcga gtactaaaag cctataaaaa
     4801 tagaggttgt ttgcaacctc tatttttata tattatgtga cttttagtcc gtcacgcc
//
LOCUS       DATUCG010000149         4828 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.151, whole genome shotgun sequence.
ACCESSION   DATUCG010000149 DATUCG010000000
VERSION     DATUCG010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4828
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.151"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(92..1357)
                     /gene="dltD"
                     /locus_tag="VCA20_002149"
     CDS             complement(92..1357)
                     /gene="dltD"
                     /locus_tag="VCA20_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEP1835886.1"
                     /translation="MRKQLWQIFGPVICATFLVILTLLFFPSSSRSNFKEEKQDAVAL
                     SYISFKSKYKKNRALGDKNHRFVPFFGSSEWSRFDSMHPSVLADAYQREYTPYLLGQR
                     GAASLTQYFGMQQIGAHLKNSQAIYVVSPQWFSPKGASSKAFEQYFSSDQATHFLQTS
                     AEDDYDRYAAKRFLELKSDCSLTGMFQKVASGKELSKWDREQLTWQAYLLEKQDALFS
                     RFADSGSNLGLVETEAMKLPEPFSYDRLSKVANEDGSRSTQSNDFQIDDRFYRNRLQS
                     KIKKLKQSQTKISYLQSPEYNDFQLVLNEFAKHQTDVLFVIPPVNECWMNYTGLNKDM
                     YRATVAKIKYQLTSQGFNHIADLSEQGGIDYFMQDTIHIGWQGWLELDKYIHPFLTEQ
                     QAPVRYQLKKEFLTSEWATYKGDLNLFLK"
     gene            complement(1350..1589)
                     /gene="dltC"
                     /locus_tag="VCA20_002150"
     CDS             complement(1350..1589)
                     /gene="dltC"
                     /locus_tag="VCA20_002150"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEP1835887.1"
                     /translation="MDVKETILNIIEELFMEDVSEMMDEDLFDAGVLDSMGTVELIVE
                     LESRFNITVPVSEFGREDWNTANKIISGVVELMHA"
     gene            complement(1632..2873)
                     /gene="dltB"
                     /locus_tag="VCA20_002151"
     CDS             complement(1632..2873)
                     /gene="dltB"
                     /locus_tag="VCA20_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009659556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEP1835888.1"
                     /translation="MMEFFKQLPQLEPYGNPLYFFYLVLALVPIFIGLFFKKRFPLYE
                     TGVSLAFIVLMFTGTKTMQLLSLLAYIIWQTILIFFYKHYRQRANQSWVFYLIVCLAV
                     FPLTWVKLAPTFSQHGAIFGFLGISYLTFRSVGMVIEMRDGLLTEFSLGSFLRFLLFM
                     PTISSGPIDRYRRFTEDYKQIPERTELLNMLDQTVHYIMMGFLYKFILAYFIGHTLLE
                     PLKAVALDQGGWFNLPTIGVMYLYGFELFFDFAGYSMFAIGISNLMGIRCPINFDQPF
                     KSRELKEFWNRWHISLSFWFRDFVFMRLVKTLLKHKVFKNRNTVSNVAYLLNMLLMGL
                     WHGVTWYYIAYGLFHALGLIINDAWIRKKKSINLARKKAGQEPLPDNRWTSFAGMFVT
                     FHTVMFSFLIFSGFLDKLWFK"
     gene            complement(2870..4405)
                     /gene="dltA"
                     /locus_tag="VCA20_002152"
     CDS             complement(2870..4405)
                     /gene="dltA"
                     /locus_tag="VCA20_002152"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEP1835889.1"
                     /translation="MISSMLEQVEHFATENSDYPVYHYLGQLYSYGDLKADSDSLAAH
                     IDSLDLPANSPVLVFGGQEYQMLAIFVALTKAGHPYIPVDSHSALNRIEAILEIAEPS
                     LVFAVADFPLETSLPVLGLSEVEAIFEKKSTYQLMHPVSGDDTYYIIFTSGTTGKPKG
                     VQISHDNLLSFTNWMIRDKEFALPERPQMLAQPSYSFDLSVMYWAPTLALGGTLFALP
                     KELTLDFKTLFAAIQTLPFKIWTSTPSFVDMALLSREFDGEHLPDLTHFYFDGEELTV
                     KTAQKLRERFPKAVIVNAYGPTEATVALSAVAITDEMLVNYKRLPIGYTKPDSATFIL
                     DEDGQVVPNGQQGEIVVSGPAVSKGYLNNPERTEAAFFQFAGLQAYHTGDLGVMTDKG
                     LLLYGGRMDFQIKYNGYRIELEEVAQHLNKSTYIEAAVAVPRYNADHKVQNLLAYVVP
                     KAGIMEQFDRELELTKVIKQELKERMMDYMMPSKFIYRDSLPLTANGKIDIKGLIAEV
                     NAQ"
     gene            complement(4422..4547)
                     /gene="dltX"
                     /locus_tag="VCA20_002153"
     CDS             complement(4422..4547)
                     /gene="dltX"
                     /locus_tag="VCA20_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEP1835890.1"
                     /translation="MQHPVVRFICRTLFYFFILLLLLYLYSYLDIGQGGFIYNEF"
BASE COUNT         1590 a         1067 c          779 g         1392 t
ORIGIN      
        1 taatcattca gtttattaca gtaaaagaac aaaaaagcaa ggagacttcc taattcaaag
       61 ccttcttgct ttttattata actgtatcta actatttcaa aaataggttt aaatctccct
      121 tatatgtagc ccattcggaa gttagaaatt ctttttttag ctgataacga acaggtgctt
      181 gttgttctgt caagaatggg tggatatact tatcaagctc cagccagcct tgccaaccga
      241 tgtggatcgt atcttgcata aaataatcta tccccccctg ctccgacaaa tcagcaatat
      301 gattgaaacc ttgactggtc aactggtact taatctttgc tactgttgcc cgatacatat
      361 ctttattgag accagtgtag ttcatccaac attcattaac tgggggaatt acaaatagca
      421 catcggtctg atgtttagca aattcattca aaaccagctg aaaatcattg tactccggtg
      481 attgcagata agatattttt gtctgggatt gttttagttt tttgattttt gattgaagac
      541 gatttctata aaacctatca tcaatttgga aatcatttga ttgagtagaa cgactcccgt
      601 cctcattagc cactttactc aagcggtcat aggaaaaagg ctctggaagt ttcatcgctt
      661 ccgtttctac caatcctaaa ttactaccag aatcagcaaa gcgactaaat aaggcatctt
      721 gcttttctaa taaataggct tgccaggtca actgctctct atcccatttc gacaattcct
      781 taccactagc tactttttga aacattcctg tcaaactaca gtctgattta agctctaaga
      841 aacgctttgc tgcatatcta tcatagtcat cctcagcact tgtttgcaga aaatgagtag
      901 cttgatcact actaaaatat tgttcaaatg ccttagatga tgcccctttg ggtgaaaacc
      961 attgaggaga aacaacatag atagcctgac tattttttaa atgtgcccca atctgttgca
     1021 tgccaaaata ttgtgtcagc gaagctgcac cacgttgtcc taacaaatag ggagtatact
     1081 ctctctgata ggcatctgcc aacactgacg gatgcatact atcaaaacgt gaccattcac
     1141 ttgacccaaa gaaaggaaca aagcgatgat ttttgtctcc cagagcccta tttttcttat
     1201 atttgctctt aaaactaata taagacaatg ctactgcatc ttgtttttct tctttgaaat
     1261 ttgaccgaga agaactcggg aaaaacaaca aggtgagaat aactaaaaac gttgcacaaa
     1321 tcacaggtcc aaaaatctgc cacaattgct tacgcatgca tcaactccac aacaccagaa
     1381 atgattttgt tagctgtatt ccaatcttca cgaccaaact ctgaaactgg aactgtaata
     1441 ttaaaacgac tttctaactc cacaataagt tctacggtcc ccatactatc caaaacaccc
     1501 gcatcaaata ggtcttcatc catcatttca gatacatctt ccataaataa ttcttcgata
     1561 atattcaata ttgtttcttt tacatccatt ttatattcct ttctacgatt catacaatcg
     1621 ttacctattg tctacttgaa ccacaattta tctaaaaatc ctgaaaatat caagaaagag
     1681 aacatgaccg tatggaaggt cacaaacata ccagcgaaac tcgtccaacg attatccggc
     1741 agtggttctt gcccagcttt ttttcgagct aaattaattg atttcttctt acgaatccaa
     1801 gcatcattga tgatcaaccc caaagcatga aagagaccat atgcaatata ataccatgtt
     1861 acaccatgcc acaagcccat caagagcata tttagcagat aggcaacatt agataccgta
     1921 tttcgatttt tgaaaacctt atgtttcaac aaagtcttaa ccagacgcat gaagacgaaa
     1981 tcacggaacc aaaaagataa gctgatatgc catcgattcc aaaattcctt aagttcacgc
     2041 gatttgaacg gttgatcaaa gttaattggg cagcgaatac ccataagatt ggaaatacct
     2101 atggcaaaca tggaatagcc tgcaaagtca aagaataatt caaaaccata aagatacata
     2161 actccaatag taggtaagtt aaaccatcca ccttgatcca aggctactgc ctttaaaggc
     2221 tccagtaaag tatgcccgat aaaataggct agaataaact tatataagaa gcccatcata
     2281 atataatgca cagtctgatc gagcatattt aataactcag ttcgctcagg aatttgtttg
     2341 taatcttccg taaaccgtct ataacgatca ataggaccac tcgaaatagt tggcataaat
     2401 agcaaaaatc gtaaaaaact ccctaaacta aattccgtta gtaaaccgtc ccgcatttca
     2461 atcaccattc cgaccgaccg aaacgttaaa taagaaatac ctaaaaaacc gaaaattgcc
     2521 ccatgctgac taaaagtcgg tgccaacttt acccaagtca acggaaaaac agctaaacac
     2581 acgattagat agaagaccca gctttgattt gcccgttgac gataatgttt gtagaaaaaa
     2641 atcaatattg tctgccaaat aatataggcc aataaagaca gcaattgcat tgtctttgta
     2701 cctgtaaaca tcaagacaat gaaggctaaa ctaacacctg tttcatacaa ggggaaacgt
     2761 tttttaaaaa agagtcctat aaaaattggt actagcgcca agactagata gaaaaagtac
     2821 aatggatttc cataaggctc taactgaggt agctgtttga aaaactccat cattgagcat
     2881 tcacctcagc gatcaacccc ttaatatcaa ttttcccatt tgcagtcaaa ggaagactgt
     2941 ctcgataaat aaacttcgaa ggcatcatat aatccatcat ccgttctttt aactcctgct
     3001 tgatgacctt ggttaactcc aactctcgat cgaactgctc cataatccct gctttaggga
     3061 caacataagc taacagattt tgcactttat ggtcagcatt ataacgtgga acagctaccg
     3121 cagcctcaat ataggttgat ttatttaagt gttgggcaac ttcttccaac tcgatacgat
     3181 aaccattata tttaatttgg aaatccatac gcccaccata gagtaacaga cctttatctg
     3241 tcattacacc caaatcccca gtatgatagg cctgtaagcc ggcaaactgg aaaaatgccg
     3301 cctcggttct ctcaggatta ttcaaatatc ctttagagac agcaggacct gatacaacaa
     3361 tttctccctg ttgtccattc ggtactactt gaccatcctc atccaaaata aaagttgccg
     3421 agtctggttt ggtatagcca atcggcaaac gtttataatt caccagcatc tcatcagtga
     3481 tggcaactgc tgataaggcg actgtagctt cggtcggtcc ataagcgtta acaatcacag
     3541 cttttggaaa acgttcgcgg agcttctgag ctgtttttac agttaactcc tctccgtcaa
     3601 aatagaaatg cgtcaaatct ggtaaatgtt ccccatcaaa ctcacgagat agcaaagcca
     3661 tatctacaaa tgatggtgtc gaagtccaaa tcttaaatgg caaagtttga atagctgcaa
     3721 ataaggtctt aaaatccaaa gtcaactctt tcggtagggc aaataaggtc ccaccaaggg
     3781 caagcgtcgg agcccaatac ataaccgaca agtcaaacga ataggaaggt tgagccaaca
     3841 tttgcggtcg ctctggtagc gcaaactcct tatcccttat catccagtta gtgaagctga
     3901 gcaaattgtc atgcgaaatt tgcacccctt ttggtttacc cgttgtccca gaagtgaaga
     3961 taatatagta ggtatcatct ccacttacag gatgcatcaa ctgataagtt gacttcttct
     4021 caaaaatggc ttcaacttca gacaaaccaa gcactggcaa acttgtctct agcggaaagt
     4081 ctgctaccgc aaacaccaaa ctcggctctg ctatttccag aatagcttca atccgattca
     4141 aagcagaatg actatctaca gggatatacg gatggccagc cttggttaag gcaacaaaga
     4201 ttgccaacat ctggtactcc tgtccgccaa aaacaagaac aggagagttt gctggtaaat
     4261 ccaaactatc aatatgggct gctaaagaat ctgaatctgc ctttaaatcc ccataactat
     4321 acaactgtcc tagataatgg taaacagggt agtcagagtt ttctgtcgca aaatgctcta
     4381 cctgctctaa catactgcta atcattttta tactctcctc tttaaaactc attatagata
     4441 aatcctccct gtccaatgtc taaataacta tacagataca aaagtagtaa caatataaaa
     4501 aaatagaaca gggttctaca gataaatcgt acaactggat gttgcatgtt ctatctcctt
     4561 ccatctctta ttcctgcgaa ttgctttaaa tacattgtct agtcaatctt gacttacttc
     4621 gaactcattc cattcattag atgattaatt ttaaatcaac ctatatttta ccataatttt
     4681 gtcaatttat ttcttttttc actaaaaaat acaagaatat acctgttaca caaagactac
     4741 ccgtggtgct agttcatttc aaaatagcgt aagttatgag ctagaataat ttgttcaatt
     4801 cgtaactgta gtccggctag gcttctag
//
LOCUS       DATUCG010000150         4785 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.152, whole genome shotgun sequence.
ACCESSION   DATUCG010000150 DATUCG010000000
VERSION     DATUCG010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4785
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.152"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(14..688)
                     /locus_tag="VCA20_002154"
     CDS             complement(14..688)
                     /locus_tag="VCA20_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEP1835891.1"
                     /translation="MLKFIKHVALLFIYFVAYQIASGFLMVGPTLQSIQDIPTQLIDS
                     TIWICAIIGLVLSIALIILLWKYIYPRHSVDYRVTALWFHKIQWPILLYIAFFIFQFI
                     VPVPESENQKLVIEFVSAYPLIAFSSVVVLAPILEELIFRGFFATYFFPKMADMKAVG
                     IYLLVTGSLFSLVHMPATLPQFLIYFTMGLNLGWLYLIRRDIRYPIALHMLNNGISYL
                     MILFLV"
     gene            complement(698..1015)
                     /locus_tag="VCA20_002155"
     CDS             complement(698..1015)
                     /locus_tag="VCA20_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835892.1"
                     /translation="MELFLWILVGFLFFNSLSDRKKVKQLQARVKKLQKTTKGENQMS
                     VLLKELVESKTKIRFDEEFSIAYEYTILAVDEEWVKISRELANGEIETKLVRVDNIVD
                     LSF"
     gene            complement(1025..1714)
                     /locus_tag="VCA20_002156"
     CDS             complement(1025..1714)
                     /locus_tag="VCA20_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEP1835893.1"
                     /translation="MNRLGSVKDWNVVKNWKFLLVGLAVVALNVLTQMAMFILPNFDG
                     NNLLIAAAFLLVAVVFGLILTGKLGLWKGEQKWGMVANIGFVVLAFIVMFGLKIIGGQ
                     LIMLEEGYGQTTANQEIINNSGLPSLVLFLFAVFFAPVLEELLFRGILMGRVFGKDSI
                     VGLLLSSFLFGLIHNPTNIGSWVVYGGMGLVLGLAYRISGKYTNALILHSLNNLLGFL
                     LMLLMQSLGLI"
     gene            complement(1740..1946)
                     /locus_tag="VCA20_002157"
     CDS             complement(1740..1946)
                     /locus_tag="VCA20_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835894.1"
                     /translation="MKENKKFISVLKIVYFVGLIGLGLYAVGYIFGEWLATGQIDVST
                     LNILLPMVLGLPALLLIEKESNEN"
     gene            complement(1968..2726)
                     /locus_tag="VCA20_002158"
     CDS             complement(1968..2726)
                     /locus_tag="VCA20_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HEP1835895.1"
                     /translation="MKQGQQISTKQRWMRYSAYMLISAILGGFIGFFGVLIFKFNPPS
                     YLNLDNFLICLRIVTFVIFAGTVYLGLKANQSYKLYHSISDEDEERIDELYKKMYRNL
                     EYATISFNVAVSLTLLNLVLGFGVTFFEDGAIMYGSIFDVVFYVILLISQIFIMKLTQ
                     KMRDYKLSAFATVKEMKDFAEAMDEGEKQANYEMSFQIVFTLNQIVLPGLYLFLFVLS
                     MLLQERQITAFLVVAFLHIYINVMQVRMIRRYFK"
     gene            complement(2723..2938)
                     /locus_tag="VCA20_002159"
     CDS             complement(2723..2938)
                     /locus_tag="VCA20_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP1835896.1"
                     /translation="MEYVLKNRLKELRARDSLNQTELAKLAEVSRQTISLIERGEYTP
                     SVVIAMRIAQIFNENVENVFQLVEVGE"
     gene            complement(2951..3517)
                     /locus_tag="VCA20_002160"
     CDS             complement(2951..3517)
                     /locus_tag="VCA20_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HEP1835897.1"
                     /translation="MEAKKKLFEVNIMENKKFVWGLNILAVVLIFPFAFVFGMIGSWL
                     IANGSQYLELTVSEVWLLFPLYILLIVVHEAIHGIFFKVFCPKNPVKYGFKWKSGMAY
                     ATSPGSLYNRMQMLVISLAPFVVISLGLTMLAGFGMMNTTLYFMLATMHAASCAGDFY
                     YAYLLLVKFTKENIAVEDTETGLIIYQA"
     gene            4016..4785
                     /locus_tag="VCA20_002161"
                     /pseudo
     CDS             4016..4785
                     /locus_tag="VCA20_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397514.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT         1580 a         1070 c          791 g         1344 t
ORIGIN      
        1 ctttacatat tgtctatacc aaaaatagaa tcatcaaata agaaataccg ttgttgagca
       61 tatgtagcgc aatcggataa cgaatatcac gtctaatcag atagagccaa ccaagattga
      121 gtcccatcgt gaaataaatc aaaaattgag gaagagttgc tggcatatgg accagactaa
      181 ataaagaccc cgttacaagt agatagattc caacagcctt catatctgcc attttaggga
      241 agaaataggt cgcaaaaaat ccacgaaaaa tcaattcttc taaaattgga gctaaaacca
      301 ctactgagct aaatgcaatc aagggataag ctgatacgaa ttcaatcaca agcttttggt
      361 tttcactttc tgggactggt acaataaatt ggaagataaa aaatgcaata tagagtaata
      421 tcggccattg aatcttatgg aaccataaag ctgtcacacg atagtcaaca gaatgacgtg
      481 gatagatata cttccataac agaataatga gcgcaatgga gagaacaaga ccaattatcg
      541 cacaaatcca aattgtactg tcaatcaact gggtaggtat gtcttgtatg ctttgtaaag
      601 ttggtcctac catcagaaaa cctgaagcaa tttgataggc aacaaagtat ataaatagta
      661 atgcgacgtg tttgataaat ttcaacataa tccctcccta aaagctcaag tcaacaatat
      721 tatcgactcg taccaacttg gtttcaatct ccccattcgc taattcacga gaaattttaa
      781 cccattcctc atcaactgcc aaaatggtgt attcataagc aatagaaaat tcctcatcaa
      841 agcggatttt ggtcttactt tcgaccaatt ctttcaataa aacggacatc tgattttctc
      901 cttttgtagt tttctgcaac ttttttaccc tagcttgcaa ctgcttcact tttttcctat
      961 ctgatagaga attgaaaaat agaaatccta ctagaatcca taaaaatagt tccatcctat
     1021 cctcctatat caagcccaga ctctgcatca gtagcatcaa caaaaatcca agaagattat
     1081 tcagactgtg tagaataagt gcattggtat atttgccaga aatacgatag gcgagtccca
     1141 ggaccagtcc catacctccg tagacaaccc aagatccgat attggtcgga ttgtgaataa
     1201 gtccaaacaa aaaactcgat agcaataatc ccacaatcga atccttacca aatactctcc
     1261 ccataagaat ccctctgaaa agcagttcct ccaaaactgg agcaaagaat actgcaaaga
     1321 gaaacaaaac taagcttggt aagcctgaat tattgataat ttcctgattg gcagttgttt
     1381 gaccgtaccc ctcttccagc ataatcaatt gcccaccaat tattttcaga ccgaacataa
     1441 cgataaaagc gaggactaca aaaccaatat tcgcaaccat cccccatttt tgctcacctt
     1501 tccacagacc taatttacca gtcaagataa gaccaaacac tacagcaacc aacaagaagg
     1561 cggcagcaat cagaaggtta ttcccatcaa aatttggtag gataaacata gccatctggg
     1621 tcaatacatt aagggcaacg acagctagtc ccactaagag aaacttccaa tttttaacca
     1681 cattccaatc tttgacactt ccaagtctat tcatagcaat ctccttcaca tacactaagt
     1741 cagttttcat tactttcttt ttcgatcaat agcagagcag gcaagcccaa aaccatcggc
     1801 aagaggatat tcagagttga cacatcaatt tgaccagtag ccagccattc tccgaaaata
     1861 tagccaactg cgtacagacc aagaccgatt aaccccacga agtaaacaat ttttagaacc
     1921 gatataaact ttttattctc tttcataatc actaccaaaa cctttcctta tttaaaatac
     1981 cgacggatca tgcgaacctg catcacattg atataaatgt gcaaaaacgc cacaactaga
     2041 aatgccgtaa tttgtcgttc ctgcaatagc atgctaagta caaataggaa tagatacaaa
     2101 cctggtaaca cgatttgatt caaagtgaag acgatttgga aactcatttc gtagtttgct
     2161 tgtttctcac cttcgtccat cgcctcagca aagtccttca tttcttttac cgtagcaaaa
     2221 gcggataact tatagtcacg cattttctga gttaatttca tgataaaaat ctgagaaatg
     2281 agaagaatga catagaaaac tacatcaaaa attgacccgt acatgatcgc cccgtcttcg
     2341 aagaaggtaa caccaaatcc tagaaccaaa tttaacagag tcaatgatac ggcaacatta
     2401 aatgatatgg tcgcgtattc taggttacga tacattttct tatatagttc atcaatacgt
     2461 tcttcatcct catctgaaat ggaatgataa agtttatacg attgattggc cttaagccca
     2521 agatatacag ttccagcgaa aattacaaat gtaacgatac gcaaacaaat tagaaagtta
     2581 tctaaattga gataagaagg tgggttgaat ttaaagatta atactccaaa gaaaccgata
     2641 aaaccaccca aaattgctga aatcagcata tacgctgaat atctcatcca tctttgtttc
     2701 gttgaaattt gttgcccctg tttcattctc ctacctccac taattgaaaa acattctcca
     2761 cattttcgtt aaaaatttga gcaattcgca tggcaatgac caccgagggc gtatattctc
     2821 ctcgctcaat caagctaata gtctgccgtg atacttctgc taactttgcc aactctgtct
     2881 ggttgaggct atcccgtgcc ctcaactcct tgagccgatt ttttaagaca tattccataa
     2941 attttccttt ctaggcttgg tatataatca agcctgtttc tgtgtcttcg acagctatat
     3001 tttccttggt aaacttgacc aataagagat aggcatagta gaaatcgcct gcacaagagg
     3061 ccgcatgcat ggttgccagc atgaagtaaa gagtggtatt catcatccca aatcctgcta
     3121 acatggtcaa gcccaaacta atcacaacaa agggagccaa gctgattact agcatttgca
     3181 tccgattgta gagcgaacct ggactggtcg cataggccat ccccgatttc catttaaacc
     3241 cgtatttgac aggatttttg ggacaaaaaa ctttgaagaa gatgccgtgg atagcctcgt
     3301 gcacaactat taaaagaata tagagaggga aaagcaacca tacctccgac acggtcagtt
     3361 ccaagtattg cgaaccgtta gctatcaacc aactgcctat cattccaaat acgaaggcaa
     3421 atggaaagat gagtaccaca gcaagaatat tcaatcccca cacaaacttc ttattttcca
     3481 tgatattgac ttcaaataat ttctttttcg cttccatggt tcttctcctt cattttccta
     3541 caagctagac taaactatat agtcctagag taactccaat cagtaagccg ataataattg
     3601 cccctgtaat cataatcgct gccctatctg ctgaaatgta tcgtttcatg gtctatctcc
     3661 tcgtaaaata agtttatggg gatttttcaa ccccaaattt ttaataattg taataacgtt
     3721 aacttgacta ttgcaaggtt ttccagaaca atagagtcat ggaatggact catggttgtt
     3781 tctttttctc cttgttgctt agacttcatt tttaagtctg ttacccaagc gttgttccta
     3841 cttccaacac actagctgtt tccattacgc tcacttctgt acgtagtagc gtacaggcgg
     3901 caggtgagct agtgattaga attctcttat gtgaggctgt tggtccaacg tgtttctggg
     3961 gaaccttaca atagtcaaga aactttccaa atacaaatga aaggagacct tccaaatgaa
     4021 atgttttgtc ggtttagacg ttagctctac caaattagat gtctgtatca tgctgagtga
     4081 tacaacaact ccctttacag cttctcttcc taatgaccta gtaggtgctt ctgaaatcaa
     4141 gaaacaaatt cttgaactca atgactccta ttcatttgaa cgcatcgtca ttggaatgga
     4201 agctaccagc ctttatagct ttcaccctgc catgttcttt catgaagaca gcgacttgaa
     4261 agctctaaag gttgaagtca tggtcgaaca acccaataaa attaagaaat atcggaagcc
     4321 tttgaagaaa gtaaaaatga taccattgat gccttctaca ttgccgatta tttccgtatc
     4381 aagcgattct cacctgcttt tctcaaggaa gagaaataca tggcgctcca gcacttgacc
     4441 agaaccaggc ttcaactcat tgagcagttg accaagacca agcagcactt tattgagaac
     4501 atctactaca agtgcaatac cttatcaact gaaatcaaga atgagaacct ctcaaccaat
     4561 ctctggtcta gcactatcat ctctctaatg acagaagact actccctaga cgaattagcc
     4621 actattgctc tcgaagatct ggcagaattt ctccagaaac taggaagagg acgattcaaa
     4681 gcacctgaaa agctagctaa agccattcaa gcggccattc gtggttctta tcgtctctct
     4741 aaactccaac aggattctgt caatgtcatt cttgggctat tggct
//
LOCUS       DATUCG010000151         4772 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.153, whole genome shotgun sequence.
ACCESSION   DATUCG010000151 DATUCG010000000
VERSION     DATUCG010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4772
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.153"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(222..1772)
                     /gene="guaA"
                     /locus_tag="VCA20_002162"
     CDS             complement(222..1772)
                     /gene="guaA"
                     /locus_tag="VCA20_002162"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEP1835898.1"
                     /translation="MTKQDVQKIIVLDYGSQYNQLISRRIREFGVFSELKNHKITAEE
                     VRAINPIGIVLSGGPNSVYAENAFDIDPEIFELGIPILGICYGMQLITHKLGGKVLPA
                     GEAGNREYGQSNLQLKAESALFANTPEEQLVLMSHGDAVTEIPEGFHLVGLSADCPYA
                     AIENTERRIYGIQFHPEVRHSVYGNDILKNFAFGICGAKGDWTMENFIETEIEKIRQT
                     VGDKKVLLGLSGGVDSSVVGVLLQRAIGDQLTCIFVDHGLLRKNEGDQVMEMLGGKFS
                     LNIIRVDAAKRFLDLLAGVSDPEKKRKIIGNEFVYVFDDEASKLTDVKFLAQGTLYTD
                     IIESGTDTAETIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRALGTALGMPDEVVWRQ
                     PFPGPGLAIRVMGEITEEKLQTVRESDAILREEIAKAGLDRDVWQYFTVNTGVRSVGV
                     MGDGRTYDYTIAIRAITSVDGMTADFAKLPWDVLQKISVRIVNEVDHVNRIVYDITSK
                     PPATVEWE"
     gene            2048..2746
                     /locus_tag="VCA20_002163"
     CDS             2048..2746
                     /locus_tag="VCA20_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003076949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEP1835899.1"
                     /translation="MKAAYITIHDKIKEQIDNGIWKIGQRLPSERDLADEFGVSRMTL
                     RQGITLLVEEGILQRKIGSGTYVASTRVQEKMRGTTSFTELVQLQGKTPSSKLLSYTR
                     TKPNEKEVEQLGLSRGEYVIRMERVRYADNVPVVYEVASIPERLIKNVPKEDVTNHFF
                     KTLMDNGYRIGTSKQTIFARLANEKVAKYLQISKNQAILALKQVSYLEDGQAFEFVNS
                     QYVGERFEFYLENN"
     gene            2849..3181
                     /locus_tag="VCA20_002164"
     CDS             2849..3181
                     /locus_tag="VCA20_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEP1835900.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     FQVSRQAVYDNIKRTEKLLEDYEMKLHMYSDYVVRSQIFDEILNKYPEDAYLKEKITV
                     LTSIDNRE"
     gene            3194..4768
                     /gene="ffh"
                     /locus_tag="VCA20_002165"
     CDS             3194..4768
                     /gene="ffh"
                     /locus_tag="VCA20_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEP1835901.1"
                     /translation="MAFESLTERLQSVFKNLRRKGKISESDIQEATKEIRLALLEADV
                     ALPVVKDFIKKVRERAIGHEVIETLNPAQQIIKIVDEELTAVLGSETSEIIKSPKIPT
                     IIMMAGLQGAGKTTFTGKLANKLKQEENARPLLIAADIYRPAAIDQLKTLGQQIDVPV
                     FELGNQVPALEIVHQGLEQAKANHNDYVLIDTAGRLQIDQALMAELREIKEFAQPNEI
                     LLVVDAMIGQEAANVAREFNEQLAITGVILTKIDGDTRGGAALSVRHITGQPIKFTGT
                     GEKITDIETFHPDRMSSRILGMGDMLTLIEKASKEYDEKKSLELAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMIPGMAGNPALANLKVDEREIARKRAIVSSMTPAERENPD
                     LLTPSRRRRIANGSGNSFVEVNKFIKDFNQAKTMMQGVMSGDMNKMMKQMGINPNNLP
                     KNMPNLNGMDMSALEDMMGGAGLPDMSQLMGGAGMPDMSQMFGGGLKGRIGEFAMKQA
                     MKRQANKIKKAKKKRK"
BASE COUNT         1486 a          953 c         1004 g         1329 t
ORIGIN      
        1 tttcttgagg catttttgtc ctgctcttgt ttcagttgac tatagttaga gctttaagct
       61 agaaaacaaa aaaacaatca aagtagtagc actaaactac tctttgattg ttttttataa
      121 gactctttcc tattttggag cgtctggtta attgatttcc cagatcagcg acgctttttt
      181 atttttaggc gtcgggtaaa acagtctatc cccagactgt tttactccca ctcaactgtt
      241 gctggtggtt tacttgttat atcatagacg atgcggttga cgtggtcgac ttcgtttaca
      301 atgcggacgg agattttttg aagaacatcc catggtagtt tggcaaagtc agctgtcatt
      361 ccatcgacag aagtgatagc acggatggcg atggtgtagt cgtaggtacg accatctccc
      421 atgacaccta cggagcgaac gccagtatta accgtgaaat attgccacac atcgcggtca
      481 agacctgctt ttgcaatttc ctcacgcaag atagcgtcag attcgcgaac agtttgaagt
      541 ttttcctcag tgatttcacc catgacacga atggcaaggc ctggtcctgg gaatggttgg
      601 cgccatacaa cttcatccgg cataccaaga gcagtaccga gggcacgaac ttcatcctta
      661 aagagagtat tgagcggttc aatcaacttg aactgcatat cttctggcag accaccaacg
      721 ttgtggtgag atttgattgt ctcagcagta tccgttcctg actcgataat atcggtataa
      781 agcgttcctt gcgctaggaa tttaacatca gtcaacttgc tcgcttcatc gtcaaatacg
      841 taaacaaatt cattaccgat gattttacgt ttcttctcag ggtcagatac gccagcaagt
      901 aggtctaaga aacgcttagc agcatcaacg cgaataatat tgaggctaaa tttaccgccc
      961 aacatttcca taacttgatc gccttcattc ttacgaagaa gaccgtggtc aacgaagata
     1021 caggtcaatt ggtcaccaat agcacgttga agaagcacac caacaacaga tgaatccaca
     1081 ccgcctgata aaccgagcaa gactttcttg tccccaacag tctggcggat tttttcaatt
     1141 tctgtttcga tgaagttttc cattgtccag tcgcctttgg ctccacagat accaaacgca
     1201 aagtttttca aaatatcgtt tccatatacg gagtgacgaa cttctgggtg gaactggata
     1261 ccgtagatac ggcgttccgt gttttcaatg gccgcgtaag ggcagtcagc agaaagacca
     1321 accaagtgga aaccttcagg gatttccgtt acggcatcac catggctcat cagaaccaat
     1381 tgctcttcag gtgtgtttgc aaataaggct gattcagctt tcaattgcaa gtttgattga
     1441 ccatattcac ggtttccagc ctcgcctgca ggtaatacct taccacccaa tttatgggta
     1501 attaactgca taccatagca aatacccaag attggaatac ctaattcaaa gatttctggg
     1561 tcaatatcaa aggcattttc agcgtagaca gagtttggac caccagagag aacgatacca
     1621 atcggattga tggcacgaac ttcttcagca gtaatcttgt gatttttcaa ttcagaaaag
     1681 acaccaaact cacgaatgcg gcgtgagatg agctgattgt actggctacc ataatccaat
     1741 acaataattt tttggacatc ttgttttgtc atgtttttcc ctttcgtttt gaaaataatt
     1801 caaatactag tagttagttt aacataaaag cgaacatttt ccaaatttta tttgttaaat
     1861 atctgttttg caaactatga gtgataataa aaatgttagt gttcgtattt ttattgtttt
     1921 tactcacttt attttaggta atctgacttc attgtttgcc taggagaatt ttaccaatag
     1981 agtgtggcct gaattagcca aacgccttta tttttggtaa actagaaaga agagaaaggc
     2041 tagataaatg aaagcagcat atatcacaat tcacgataaa attaaggaac aaatcgataa
     2101 cggaatctgg aaaattggtc aacgtttacc aagtgaacgc gatttagcag atgaatttgg
     2161 tgtttctcgt atgacgctcc gtcaaggaat cacactttta gtggaagaag gtattctaca
     2221 acgcaagatt ggttccggta cttatgtcgc cagtacacgg gttcaagaaa agatgcgagg
     2281 cacgacttcc tttactgaat tagtacaact ccaaggaaag acgccaagca gcaaattatt
     2341 gtcctataca cgaaccaaac ctaatgaaaa agaagttgaa caattaggtt tatcccgtgg
     2401 cgagtatgtt attcgaatgg agcgggtccg ctatgcagat aatgttcccg tcgtgtatga
     2461 ggttgccagc attcctgaac gcttgataaa aaatgtcccc aaagaagatg ttaccaacca
     2521 tttctttaaa acacttatgg acaatggtta tcgaattgga acgagcaaac aaaccatttt
     2581 tgcgcgttta gccaatgaaa aagtggctaa atatttacaa atttctaaaa accaggctat
     2641 tctcgcacta aaacaggtat cttatctcga ggatggtcaa gcatttgaat ttgtaaatag
     2701 tcagtatgtt ggagaacgtt tcgaattcta tttggaaaac aattaacata aaaataatta
     2761 tgtaaaacat ttttaaaatc cgtaatcttt ctagtgatga cggattttct tatttataaa
     2821 atatggaatt tttgatataa taaagtttat ggaaattgaa aaaaccaacc gaatgaatgc
     2881 cttatttgaa ttctatgcgg ccttgctaac agacaagcaa atgaactaca tcgagctcta
     2941 ttatgccgat gattatagtt tggctgagat tgctgaagaa tttcaagtca gccgtcaggc
     3001 ggtctatgat aacattaaac gaacagaaaa attactggaa gactacgaga tgaaactaca
     3061 tatgtattct gactatgtgg tgcgtagtca gattttcgat gaaatactga acaagtatcc
     3121 agaagatgct tatttgaagg agaaaatcac tgttttaacc agtattgata atagagaata
     3181 agaggagaag tatatggctt ttgaaagctt aacagagcgt ttgcagagtg tctttaaaaa
     3241 cctacgtcgt aaggggaaaa tttctgagag tgatatccaa gaagcaacca aggaaatccg
     3301 tctggcctta ctagaagccg atgttgctct tccagttgta aaagacttta tcaagaaggt
     3361 gcgtgaacgt gccattggtc acgaagttat tgaaaccctg aaccccgctc aacaaatcat
     3421 caaaatcgtt gatgaagaat tgacggctgt attaggttct gaaacttcag aaattatcaa
     3481 atcaccaaaa attccaacta tcattatgat ggctggattg cagggtgctg gtaaaacgac
     3541 cttcacaggt aagttggcta ataaactcaa gcaggaagaa aatgcccgtc cactcttaat
     3601 tgctgccgat atttatcgtc cagctgccat tgaccagttg aagacccttg gtcaacaaat
     3661 tgatgtacct gtattcgagt tgggcaatca agtccctgcc ctggagattg tacaccaagg
     3721 tttagaacaa gccaaagcta atcataatga ctacgttctg atcgatacag caggtcgttt
     3781 gcagattgac caagctctta tggccgaatt gagagaaatc aaagagtttg ctcagccaaa
     3841 cgaaatcctc ttggtcgtcg atgccatgat tggtcaggaa gctgccaacg ttgcgcgtga
     3901 gttcaatgaa caattggcta ttactggtgt gattttgacc aagattgacg gcgatacacg
     3961 tggtggtgca gccttatctg tccgtcacat tactggtcag ccaatcaaat ttaccggtac
     4021 tggtgaaaaa atcaccgaca tcgaaacctt ccacccagac cgtatgtcct cgcgtatttt
     4081 gggcatgggt gacatgctga ccttgattga gaaggcaagc aaagagtacg acgagaagaa
     4141 atcactagaa ctcgctgaaa aaatgcgtga gaataccttt gatttcaacg atttcattga
     4201 tcagctggac caagttcaga atatgggacc aatggaagac ctgctgaaga tgattccagg
     4261 tatggctggt aacccagcac ttgccaactt gaaagttgac gagcgtgaaa ttgcacgaaa
     4321 acgagctatc gtatcttcta tgacaccagc cgaacgagaa aaccctgatt tgttgacacc
     4381 gagccgtcgt cgtcgtattg ctaacggttc tggaaatagc tttgtcgaag tcaataaatt
     4441 tatcaaggat ttcaaccaag cgaaaaccat gatgcagggt gtcatgtcag gcgacatgaa
     4501 caaaatgatg aaacaaatgg gaatcaaccc aaataatctc cctaaaaaca tgccaaacct
     4561 gaacggtatg gatatgtctg ctcttgaaga tatgatgggc ggtgcaggtc tgcctgatat
     4621 gagccaactg atgggaggag ctggtatgcc agacatgtcc caaatgtttg gaggcggttt
     4681 gaaaggtaga atcggagagt ttgctatgaa acaagccatg aaacgccaag ccaataaaat
     4741 caagaaagcc aaaaagaaga gaaaataatc tc
//
LOCUS       DATUCG010000152         4724 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.154, whole genome shotgun sequence.
ACCESSION   DATUCG010000152 DATUCG010000000
VERSION     DATUCG010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4724
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.154"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            62..135
                     /locus_tag="VCA20_002166"
     tRNA            62..135
                     /locus_tag="VCA20_002166"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:96..98,aa:Pro,seq:tgg)
     gene            complement(462..1814)
                     /gene="rlmD"
                     /locus_tag="VCA20_002167"
     CDS             complement(462..1814)
                     /gene="rlmD"
                     /locus_tag="VCA20_002167"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEP1835902.1"
                     /translation="MNLQVNQRIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEEIFC
                     QITLVKRNFVEAKLLKINKESKFRVKPTCDIYQACGGCQIMHLRYDKQLDFKQDLLQQ
                     ALKKFKPAGYENYTIYPTIGMDKPSHYRAKLQFQTRSFKDQVKAGLYAENSHRLIGIT
                     NCYVQDAETQEIINGIAKLLTKHRIPIYNERKQHGIRTVMVRKAQKTGQVQVIFITSC
                     QVNLIKLIDELTSQFPSIITIALNWNRQKTSDIYGEKTEILWGEQAIDEAVLDYEFSL
                     SPRAFYQLNPQQTEILYGEAVKALDIAGDEDLIDAYCGVGTIGFAFAKKVKSVRGMDI
                     IPEAIEDAKANAKRMELSNTHYEAGKAEEIIPKWYQEGYRAEALVVDPPRTGLDEKLL
                     KTILDYKPAKMVYVSCNVSTLARDLVELSKAYKVEYIQSVDMFPHTARTEAVVKLNKK
                     "
     gene            1852..2628
                     /gene="recX"
                     /locus_tag="VCA20_002168"
     CDS             1852..2628
                     /gene="recX"
                     /locus_tag="VCA20_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEP1835903.1"
                     /translation="MRIKKIEKKKSLYLLEVDGQDSLYITEDTIVRFMLSKGKEITEQ
                     EFRELRDFAQFSYGKNLALYYLSFKQRTKKEVSDYLKKYEIEENNIVKIVTVLEEEKW
                     LVDENYVDSYVRQNALNGDKGPAMIRQKLMQKGIPKLLIDKRLAEEDFSELARKIGEK
                     LVGRYQRKLPLRALQDKVVQGLMGKGFSYETAKQSLGQLELVADEENEDDLIAKELDK
                     QYRKYSRKYEGYELKQRLIQALARKGYDFDRIQAVLRDYL"
     gene            2704..3237
                     /locus_tag="VCA20_002169"
     CDS             2704..3237
                     /locus_tag="VCA20_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEP1835904.1"
                     /translation="MKLPKEGDFITIQSYKHDGEIHRTWRDTMVLKTTENAIIGVNNH
                     TLVTENDGRRWVTREPAIVYFHKKYWFNIIAMIRENGVSYYCNLASPYVLDNEALKYI
                     DYDLDVKVFADGEKRLLDVDEYERHRKAMKYSDDIDFILKENVKILVDWINNQRGPFS
                     PAYVNIWYKRYLELRSR"
     gene            3295..3612
                     /locus_tag="VCA20_002170"
     CDS             3295..3612
                     /locus_tag="VCA20_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HEP1835905.1"
                     /translation="MAFEQTNGRYASFGVVTSLPGEVIDSFWYVIDHYLKGVIPLKSV
                     IRFSIKNRRGKITLVFSQEGYKNVLAVDLSSRFDPVYPSTILVMDKQGKETITLPDEV
                     TLL"
     gene            3712..4443
                     /locus_tag="VCA20_002171"
     CDS             3712..4443
                     /locus_tag="VCA20_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEP1835906.1"
                     /translation="MVSQTVEKPLYLQLVDELEVAIRERMAPNDKLFSERELTQVYGV
                     SRITVRLALQELEKRGLVYKKHGKGTYVSEISDTAVDLSQAYSFTEQMKKIGKVPRTS
                     ILSFELVKASDYIAQHLQLSQGEEVFEVERLRLADEIPMMLERTYVPASLFPGLAAQR
                     MKSTPLYEIFSEDYHQVIRLAEEEFYASIALDNEAKILGIPNNSPVLHLVRKTYNDKN
                     RIIEFTFSIARADQFRYKITHQRGY"
BASE COUNT         1380 a          748 c         1072 g         1524 t
ORIGIN      
        1 tcctctaggg tgcatcactc gcttaatgga gacgttaggg gagctttatt tttaatagta
       61 tcgggaagta gctcagcttg gtagagtact tggtttggga ccaaggtgtc gcaggttcga
      121 atcctgtctt cccgattact gtgagagagc agcaatgttc ttttttagct ctttgtcaac
      181 tgtagtgggt agatgaaaag ctaacaccta gagaggacga acttcgttct ctcttttttg
      241 gtgtctaagg caatcaaaat ccgtttttct agttttcatc ttcctgaata caaaggcatt
      301 tttttaatga ctttgatgag attgttagtt gtttttgatt tggtgttgga aaatgtgagt
      361 tttgttgttt ttcagtgtag gtcatagggg actttttttc tacactcaaa aaggctctat
      421 atgaccatag tggttttacc cgtcatagtc ttataggcct tttatttttt gtttagttta
      481 acaaccgcct cggtccgtgc tgtgtgggga aacatgtcga cagattgaat gtattctacc
      541 ttgtaggctt tgcttagctc aacaaggtcc cttgcaagag tggagacgtt gcaggataca
      601 taaaccattt tagctggttt gtaatccaaa atggttttga ggagcttttc gtcgagtcca
      661 gttcgtggag gatcgactac caaggcctcg gccctgtagc cttcttggta ccactttgga
      721 atgatttctt ctgctttacc tgcctcatag tgggtattgg atagttccat tcgcttagcg
      781 tttgctttag catcctcgat ggcttctgga ataatgtcca tacctcgaac agatttgacc
      841 tttttagcaa aggcgaagcc gattgtgccc acgccacagt aggcatcaat caagtcttca
      901 tctcctgcaa tatccaaagc cttgacagcc tcgccgtaga gaatttctgt ttgttgggga
      961 tttagttggt aaaatgcccg tggagatagt gagaattcgt aatctaaaac agcttcatcg
     1021 atagcttgtt ctccccataa aatttctgtt ttttcaccat aaatatcact cgttttctgt
     1081 ctattccagt ttagagcaat agtgattata cttggaaatt gactggtgag ttcatcaatt
     1141 aatttaataa gattaacctg acaagaggtg ataaagatca cttgaacttg acctgttttt
     1201 tgagccttgc ggaccataac cgtgcggatt ccatgttgct tgcgctcatt ataaatagga
     1261 atacggtgct tggtgagtag ctttgcgatt ccgttgataa tctcttgtgt ttcggcatct
     1321 tggacgtaac aattcgtaat tccaatcaag cgatgggaat tttccgcata gagaccggcc
     1381 ttgacttgat ctttgaaaga tcgagtctgg aactgtaatt tggcacgata gtggctaggc
     1441 ttatccatac cgattgttgg ataaatagtg tagttttcgt agccagctgg tttgaacttt
     1501 tttagggcct gctgcaagag atcttgtttg aagtctaatt gtttatcata gcggaggtgc
     1561 atgatttgac aaccaccaca ggcttgatag atgtcacagg taggcttgac acggaatttt
     1621 gattccttgt tgatttttaa caattttgct tcaacgaaat ttcgtttgac taaggtaatt
     1681 tgacaaaaaa tttcttcccc cttaagggca ccagggacaa agactagcgt tttttgataa
     1741 aatccgatgc cttctccgtt gatgcccatt cgtttgattt ttaaggggat tcgttggttt
     1801 acttgtagat tcatattatt attttaccga aaaatcctgt ataatgaaag aatgagaatt
     1861 aaaaaaattg aaaagaagaa aagtctttat ttacttgaag tggatggtca ggatagttta
     1921 tatattacgg aagacacaat tgttcgcttc atgcttagta agggcaagga aatcacggag
     1981 caggagtttc gtgagcttcg cgattttgct cagttttctt atgggaaaaa tctagcactt
     2041 tattaccttt ccttcaagca acgtaccaaa aaagaagtta gtgattatct aaaaaaatat
     2101 gaaattgaag aaaacaatat tgtcaagata gtgacggtat tagaagagga aaaatggctg
     2161 gtcgatgaga attatgttga tagctatgtt cgtcagaatg ctctaaatgg agataaggga
     2221 ccagctatga ttcgtcagaa gttgatgcag aaaggaattc ctaaactgct gattgataaa
     2281 aggctggcag aggaagattt ttcagagctt gctaggaaga ttggggagaa attggtgggt
     2341 agataccaaa gaaaattgcc tcttcgagct ttgcaagata aagttgtaca gggattgatg
     2401 gggaagggtt tttcttatga gacggccaaa caaagcctgg gtcagttgga acttgtggct
     2461 gatgaggaaa atgaagatga tttaatcgcc aaggaactgg ataagcagta tagaaaatat
     2521 agtcgtaaat atgaaggcta cgaattgaag caaaggttga tacaggcttt agctcgaaag
     2581 ggttatgact ttgatcggat tcaggctgtc cttcgtgatt atctgtgaaa aaatttagcc
     2641 taggttagaa aaatatgata taatagtcaa gataaattgt aattgtagaa agttggtaac
     2701 aacgtgaaat taccaaagga aggcgacttt attacaattc aaagttataa gcatgacggt
     2761 gaaattcatc gcacttggcg tgacaccatg gtattaaaaa caacggaaaa tgctattatt
     2821 ggagtcaata atcatacctt agtgactgaa aatgatggta ggcgttgggt aacaagagaa
     2881 ccagcaatcg tttattttca taaaaaatac tggttcaata tcatagcaat gattcgtgaa
     2941 aacggagttt cctattattg taaccttgct agtccatatg ttctcgacaa tgaggcactc
     3001 aaatacattg attatgacct agatgttaag gtctttgctg atggtgaaaa gagattgctg
     3061 gatgtggatg aatatgagcg tcatcgtaag gcgatgaagt attcggatga tattgatttt
     3121 attttgaagg aaaatgtcaa gatactggtt gattggatta ataaccagcg cggaccattc
     3181 tctccagcct acgttaatat ctggtacaaa cgctatttag aactgagaag tagataaagt
     3241 tagaaaagaa aatcctgttt tacaggcttt tttcttattg taaaggaggg gcttatggcc
     3301 tttgaacaaa caaatgggcg ctatgcaagt tttggagttg tgacctcgct tcctggggaa
     3361 gtgattgata gtttctggta tgttattgat cactacttaa aaggggttat tccattaaaa
     3421 agtgtaatcc gtttttctat caaaaatcgt cgtggtaaga ttacgctggt cttttcgcag
     3481 gaaggttata aaaatgtact ggctgtagat ctaagtagtc gttttgatcc tgtttatccc
     3541 tcaacaatct tggtaatgga caagcaggga aaagagacca ttactttgcc agatgaggtg
     3601 accttgcttt agcttgaaga gctggtattt tatcacctaa gatattgtta atatatgaaa
     3661 tttggtgtaa aatagatttg taagcttatt tgggttagaa aggaggagtc tatggttagt
     3721 caaactgttg aaaaacctct ctatttgcag ttggttgatg aattggaagt tgcaattcgt
     3781 gagaggatgg ctccgaatga caagttattt tcagagcgtg agttgacaca ggtttatggt
     3841 gtcagtcgta ttacagttcg tttggctttg caagaattgg aaaaacgtgg cttagtctat
     3901 aagaagcatg gtaagggaac ctatgtatct gagatttcag atactgcagt tgatttgtct
     3961 caggcctata gttttactga gcaaatgaaa aaaattggga aagtaccaag gacatctatt
     4021 ttatcctttg aattggttaa ggcgtcagac tatattgcgc agcatttaca acttagtcaa
     4081 ggtgaggaag tgtttgaagt tgaacgattg agacttgccg atgagattcc gatgatgctg
     4141 gaaaggacat atgttcctgc ttcgctattt cctggattag ctgctcagcg aatgaagtca
     4201 actcccttgt atgaaatttt ttcagaggat tatcatcagg tgattcgttt ggcagaagaa
     4261 gagttctatg caagtattgc tttggataat gaagctaaga tactcggaat tccaaacaat
     4321 agtccagtgc ttcatttggt aagaaaaacg tataatgata agaatagaat tatcgaattt
     4381 acgtttagta ttgctagagc ggaccagttt cgttacaaga ttacccatca acggggatat
     4441 tagaattaat actcttcgaa aatcaaaatt attcgttgtc aagagccttg atgaaatcca
     4501 gttctatctt cggtttcgtt tcctaggcta cttttgattg ttattgggta tgaaaaaaac
     4561 gcggacgtta gtcaatgtca ttaagttaag catttaagca gctaaagaaa ctattcccct
     4621 ctgagcaatt gatgacaaaa cgggaatagt ttttgtattt atgccgcaca aaaaggaagg
     4681 tttttaaccc aattttttaa ctattatgat gttctataag tgaa
//
LOCUS       DATUCG010000153         4711 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.155, whole genome shotgun sequence.
ACCESSION   DATUCG010000153 DATUCG010000000
VERSION     DATUCG010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4711
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.155"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(29..1540)
                     /locus_tag="VCA20_002172"
     CDS             complement(29..1540)
                     /locus_tag="VCA20_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HEP1835907.1"
                     /translation="MKKVGLLFLSVSALLLGACGNSKASEDGKLNIVTTFYPVYEFTK
                     QVAGDEANVDLLVKAGTEVHGYEPSAKDIARIQEADAFVYENENMETWVHDVEKSLDT
                     TKVNVISATAGVLLLPGGEEEHEGHDHSGEGHSHAYDPHVWLSPERAITLVENIRDSL
                     VAKYPEKKDAFETNAAAYIEKLDALDAKYSETLSAAKQKYFVTQHTAFAYLALDYGLK
                     QVSITGVAADEDPTPSRLAELTEYINKYGIKYIYFEENASKSVAETLAKETGVQLDVL
                     NPLESLTDEDMKNGKDYISVMEDNLTALEKTTSQEGSEILPEEGAETAQTVYNGYFED
                     SAVKDRTLSDYAGEWRSVYPYLLDGTLDQVWDYKAKIKGGMTAEEYKAYYDTGYKTDV
                     DQINITDNTMEFVVGDKKEKFTYKYVGYKILTYKKGNRGVRFLFEATDANAGNYKYVQ
                     FSDHNIAPVKTGHFHIYFGGESQEKLLEELENWPTYYPVGLTGLEIGQEMLAH"
     gene            complement(1550..2362)
                     /locus_tag="VCA20_002173"
     CDS             complement(1550..2362)
                     /locus_tag="VCA20_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEP1835908.1"
                     /translation="MFDLSVFHYDFMQRAFLAIIAMSLFSPILGVFLILRRQSLMSDT
                     LSHVSLAGVAFGLVLGISPTLSTVLIVIVAAVFLEYLRTIYKNFMEIGTAILMSTGLA
                     ISLIVMNKSGGKSGLSLEQYLFGSIVTISQEQVIALFTIAVIVIVLTLLFLRPMYILT
                     FDEDTAFVDGLPVRAMSIAFNVVTGVAIALMIPAAGALLVSTIMVLPASIALRLGKSF
                     KAVIFTGMGIGFFGMVMGLLTSYYAETPASASITLIFISIFLLVNVVQKFKK"
     gene            complement(2355..3059)
                     /locus_tag="VCA20_002174"
     CDS             complement(2355..3059)
                     /locus_tag="VCA20_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEP1835909.1"
                     /translation="MRYITVEDLSFYYDKEPVLEHIHYYLDSGEFVTLTGENGAAKTT
                     LIKATLGILKPKQGKVSIAEKSIKGKKLRMAYLPQQIASFNAGFPSTVYEFVKSGRYP
                     RQGWFRRLTAHDEEHVRISLESVGMWEHRDKRLGALSGGQKQRAVIARMFASDPDIFI
                     LDEPTTGMDAGTKDAFYQLMHHSAKKHGKSVLMITHDPDELNKYADRNIHLVRDQQSP
                     WRCFNVHEADEEVAHV"
     gene            complement(3060..3503)
                     /locus_tag="VCA20_002175"
     CDS             complement(3060..3503)
                     /locus_tag="VCA20_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent MarR family transcriptional
                     regulator"
                     /protein_id="HEP1835910.1"
                     /translation="MNRIALEIEKYLHEIVLSSENQLEILVGSCQSTVKLTNTQEHIL
                     MLIEKAAYTNTEIAKELNVSQAAITKATKSLVAQGLLVAVRDDKDARIVRFSLTEAAK
                     PIAAEHAHHHAHTLEAYEELLEKYSLEEQESIARFLSELVEKIRK"
     gene            3703..3894
                     /locus_tag="VCA20_002176"
                     /pseudo
     CDS             3703..3894
                     /locus_tag="VCA20_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            4173..>4711
                     /locus_tag="VCA20_002177"
     CDS             4173..>4711
                     /locus_tag="VCA20_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HEP1835911.1"
                     /translation="MSYLQYTAKSHHLQWNVCQLSQICHHFYQNYCPDSFKHRRNVGL
                     AKVSDESLLVLLLLQAELGITSQRHFYSICHLFPCGQLLERSRFNRRSKQLIWLVQFI
                     RKAMSEMLPSDKLAIIDSFPLSLCQPVRNHRASIFKGLADIGYNASKHLWFYGFKVHM
                     LVTLSGYILNYVITPASVH"
BASE COUNT         1487 a         1145 c          762 g         1317 t
ORIGIN      
        1 actgaacatt ccgttcagcc ttattttctt agtgagccag catttcctga ccaatttcca
       61 aaccagtcaa accaacaggg tagtatgtcg gccagttttc caactcttcc aagagttttt
      121 cttggctctc tccaccaaaa tagatatgga agtgacctgt tttaacaggg gcgatattgt
      181 ggtcgctaaa ttgaacatac ttatagttac cagcatttgc atccgttgct tcaaacaaga
      241 aacgaacacc acgatttcct tttttgtagg tcaaaatctt gtagcccaca tacttatacg
      301 tgaatttttc tttcttatct cccactacaa attccatggt attatctgta atgttaattt
      361 gatcaacatc tgttttgtag ccagtatcat aataagcctt gtactcttca gctgtcatac
      421 cacctttgat cttagccttg taatcccaga cctgatctag cgtcccatca agcaagtatg
      481 gataaacaga tcgccactca cctgcataat ctgaaagagt acgatctttt acggcactat
      541 cttcaaaata accgttataa acagtttgcg cagtctcagc accttcttct ggcaaaattt
      601 cagaaccttc ttgggaagtt gttttttcaa gggcagtcag attatcttcc ataacagaaa
      661 tataatcctt accatttttc atatcctcat ctgtcaaact ttcaagagga ttaagaacgt
      721 ccaactgaac cccagtctct ttggcaagtg tttctgcaac agattttgac gcattttctt
      781 caaaatagat atacttaata ccatatttat taatatactc tgtcaattct gctagacgtg
      841 atggagtcgg atcctcgtct gcagcaacac ctgtaataga aacttgtttc aaaccatagt
      901 ccaaagccaa atatgcaaat gcagtatgtt gtgtaacaaa gtatttctgt ttcgcagcag
      961 ataatgtttc agaatattta gcatccaaag catctaattt ctcaatatag gctgccgcat
     1021 ttgtttcaaa agcatctttc ttttctgggt atttagctac caaactatca cgaatatttt
     1081 ctacaagtgt gatggcacgc tcaggagaca accatacatg cggatcataa gcatggctat
     1141 gcccctcccc actatggtcg tgaccttcat gttcctcctc accgccaggc aaaagcaaca
     1201 cccccgccgt cgcactaatc acgttaacct ttgttgtatc aagtgatttt tcaacatcat
     1261 ggacccatgt ttccatattt tcattttcat aaacaaaagc atctgcctct tggatacgag
     1321 caatatcttt agctgacggc tcataaccat gaacctctgt cccagcctta accaacagat
     1381 caacatttgc ctcatcacct gccacctgct tggtgaactc ataaacagga taaaaggtcg
     1441 ttacaatatt caacttccca tcttcagaag ctttgctatt gccacaagcc cccaaaagca
     1501 aagctgagac agacaagaat aataaaccaa cttttttcat cttaactcct tattttttaa
     1561 atttttgaac aacattcacc agtaagaaaa tactaatgaa aatcaaggta atacttgcac
     1621 tcgctggggt ttctgcatag taagaggtca gcaaccccat gaccattcca aaaaagccaa
     1681 tgcccatccc agtaaagata acagctttaa aactcttacc aagtcgcaat gcaatagaag
     1741 caggcaaaac cataattgtc gaaaccagca aagcacctgc cgcggggatc attaaggcaa
     1801 tggcaacacc agtcaccaca ttaaaggcaa tggacatagc tcgcacaggc aatccatcta
     1861 caaatgccgt atcctcgtca aaggtaagaa tatacatcgg acgtaaaaat agtaatgtca
     1921 gcacaatgac aatcacagca atcgtaaaca aagcaattac ctgctcctga ctaatggtca
     1981 ctatcgaacc aaacagatat tgttccaggc taagccctga tttcccacca gatttattca
     2041 tgacaatgag cgaaatagcc aaaccagtcg acataagaat agctgtcccg atttccataa
     2101 agtttttgta aatggtccgc aggtattcca gaaacacagc tgccacaatg acaatcagaa
     2161 ctgtcgaaag tgttggtgaa atccccaata ccaaaccaaa tgcaaccccc gccaacgaaa
     2221 catgactaag agtatccgac atcaaactct gtcgtctaag tatcaaaaag acccccaaaa
     2281 tgggcgagaa cagactcatc gcaatgattg ctaggaaagc acgttgcata aagtcataat
     2341 gaaatacaga tagatcaaac atgggcaacc tcctcatccg cttcatgaac attgaaacaa
     2401 cgccaaggag actgctgatc acgaaccaag tgaatgtttc gatcagcata cttattcagc
     2461 tcatcaggat catgagttat catcaaaacc gactttccat gcttcttagc cgaatggtgc
     2521 atgagctggt aaaaagcatc cttggtccct gcatccatcc ctgttgtcgg ttcatccaga
     2581 ataaaaatat ctggatcaga ggcaaacata cgcgcaatga ccgcacgctg cttctgccca
     2641 ccactcaaag cacctaagcg cttatcccga tgttcccaca ttccaactga ttccaaacta
     2701 atccgaacat gttcctcatc atgggctgtc aaacgacgaa accaaccttg ccgaggatat
     2761 cgccctgatt ttacaaattc gtaaaccgtg ctcggaaaac cagcattaaa acttgcaatt
     2821 tgctgaggta aataggccat tctcaacttc ttccccttta tactcttctc agcaatagaa
     2881 accttccctt gtttgggctt caatatcccc aaagtcgcct taatgagggt tgtcttggct
     2941 gcgccattct caccagtcaa agtcacaaat tccccactat caagataata atgaatatgt
     3001 tctaaaaccg gttctttgtc gtagtaaaac gacaagtctt ccacagtaat atatctcatc
     3061 tattttctaa tcttctccac taactcactt aagaatcgag caattgactc ctgctcttca
     3121 agactatatt tttccaaaag ctcttcataa gcttccaagg tatgcgcgtg atgatgagca
     3181 tgctctgcag caataggctt agcagcctca gtcaaactaa agcgaacaat acgagcatcc
     3241 ttatcatctc taaccgccac caataacccc tgagcaacta atgactttgt agccttcgtg
     3301 atcgcagcct gactaacatt caactcctta gcaatctcag tattagtata tgcggccttt
     3361 tcaataagca tcaaaatatg ctcctgcgta ttcgtcaact tcaccgtgct ttgacaagag
     3421 ccaaccaaaa tctctaattg attctccgaa ctcaaaacaa tctcgtgcag gtacttctca
     3481 atttctaatg caatacgatt cataggcatc cccttaacct tttaattaac ttgttaagta
     3541 taccacatag aaaacaaaaa aacaaggaaa atatttaccc tcaaagtaag tactagtctg
     3601 actcaaaaac tagaaactag actgagacga aaaaataagg tagagtttga gaacaataaa
     3661 gtacgtttag aattgaagag tttagttgaa aatggtaaat aaaaattact ctttacagtc
     3721 ctattcttta caggtctaag acttagagaa gcactcgcac taacatggaa agacatcgac
     3781 ctttcgagca atacaattca tatcacaaag tctgtatacg ttaataaagg tataagccat
     3841 atcagtgcaa ctgaaactaa agcaggaact agaagaaata tcatcaataa aaaatcatga
     3901 ccacccgtca aacgggtggt ttgaacaagg gctataagcc cacatcgcca gccagcgcct
     3961 aaagacgctg gctttcactt tgttcaagct tcactgcttt tgactcgtca ctagcctctt
     4021 aaagaggcat tcgtactact tgccattatc cctaaaggga tcttcatact cttttacact
     4081 caatacccgt ggtgctagtt catttcaaaa tacaataaaa agcccaaatg gactatactg
     4141 taagtgacga aacatacagg aggttagtcc aaatgagcta cttacagtat accgctaaat
     4201 ctcatcattt gcaatggaat gtatgccaat tatcacaaat ttgtcatcac ttctaccaaa
     4261 actattgtcc agattccttt aaacatcggc gtaatgttgg cttagccaaa gtttcagatg
     4321 aatcactcct tgtcttgctc ttgttacagg ccgaactagg cataacgtca cagcgtcatt
     4381 tctatagcat ctgccacctc tttccttgtg gtcagttact agaaagaagt cgtttcaatc
     4441 gtcggtcaaa acaattgatt tggctagttc aattcatccg aaaagctatg agtgaaatgc
     4501 ttccatctga taaactagcc attatagata gttttccttt gtcactttgc caacccgttc
     4561 gcaatcacag agctagcatt tttaaaggat tagccgatat tggctataac gcctctaaac
     4621 atctttggtt ctacggcttc aaggttcata tgctagtgac cttatctggt tatattctga
     4681 actatgttat cacaccagcc tctgttcacg a
//
LOCUS       DATUCG010000154         4703 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.156, whole genome shotgun sequence.
ACCESSION   DATUCG010000154 DATUCG010000000
VERSION     DATUCG010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4703
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.156"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            <1..1122
                     /locus_tag="VCA20_002178"
     CDS             <1..1122
                     /locus_tag="VCA20_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEP1835912.1"
                     /translation="ATALIARSIGAGDRESLTFHVRQALVLSVGVGMLFGLLSLVFGR
                     QMLVLMGAEMESLAGAQAFFYWVGGLTIFQALMTILGTILRASGDTVSPMKMSLLTNG
                     FNVVLDYFLIFGIGSWSGLEIVGTALGTVLARLLGTVLLYRKVQQTDLAVVLKQMFHL
                     GSPKEMVVLTLPAAAERLVMRLGQVFYFSLIVGLGTTVYASHMIAGNIESFTYMPAYG
                     LATAAAVLIGQALGKGDILTVRRVAFLSSAYGVAIMSLLGTILFFGTPSFALLFTKDL
                     EAVRQVVTALRIDAFNQPGLAVSLIMAGALQGLGDTKSPLYSTMIGMWGLRVSGVIVL
                     GQMLGLGIAGVWLSILIDLLLRAIFLTWRFHVKTRKLAE"
     gene            1301..1678
                     /locus_tag="VCA20_002179"
     CDS             1301..1678
                     /locus_tag="VCA20_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEP1835913.1"
                     /translation="MKTIHTDKAPAAIGPYVQGKVVGNFLFASGQVPLSPETGEVVGE
                     TIQEQTEQVLKNIAAILSEAGTDFDHVVKTTCFLKDMNDFVAFNEVYKTAFSADFPAR
                     SAVEVARLPRDVKVEIEVIAVID"
     gene            1700..2587
                     /gene="rapZ"
                     /locus_tag="VCA20_002180"
     CDS             1700..2587
                     /gene="rapZ"
                     /locus_tag="VCA20_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002933011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEP1835914.1"
                     /translation="MSDKLHLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPTLLPKF
                     LELIRHSQDNNKIALVVDMRSRSFFSEIREVLDEIEGAEDLDFKVLFLDATDSELVAR
                     YKETRRSHPLAADGRVLDGIQLERELLAPLKNMSQNVIDTTELTPRNLRKAISEQFAS
                     QDNQPSFRIEVMSFGFKYGLPLDADLVFDVRFLPNPYYKLELRNLTGLDAPVFDYVME
                     HQESEEFYSHLLGLIEPILPGYQKEGKSVLTIAVGCTGGQHRSVAFAKRLADDLEKNW
                     NVNRSHRDKDRRKETVNRS"
     gene            2584..3558
                     /locus_tag="VCA20_002181"
     CDS             2584..3558
                     /locus_tag="VCA20_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEP1835915.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLRDKDVEITAIVTVADDGGSSGE
                     IRQALQVTPPGDLRNVLLAMSDMPKLYEQIFQYRFADSDGPLAGHPLGNLIIAGISEM
                     QGSTYNAMRLLTRFFHTTGRIYPSSEQALTLHAIFTDGTEVAGESKISKHNGMIDHVY
                     VTNSYNDDEPKASRQVVETIMESDMIVLGPGSLFTSILPNLMISDIGKALKETKAEVT
                     YVCNIMTQRGETEFFSDADHVAVLNAHLAEQFIDTVLVNIEPVPQEYMNSNQFDEYLV
                     QVKHDFAGLQKQANRVISSKFLRLENGGAFHDGDLVVEELLKILQVRS"
     gene            3555..4472
                     /gene="whiA"
                     /locus_tag="VCA20_002182"
     CDS             3555..4472
                     /gene="whiA"
                     /locus_tag="VCA20_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEP1835916.1"
                     /translation="MSFTVQVKEELLLQSSQNKSELSAIIKLSGSLGLASSGLTLSIS
                     TENAKIARHIYELLLHFYQIKAEIRHHQKPNLKKNRVYAVLIEDGVNEILNDLHLADS
                     FFGLETGISPLVLENDSWSQAYLRGAFLAAGSVKDPEKGKYQLEIASVYSDHANDLAN
                     LMQKFLLDAKVIERSKGTITYLQRAEDIMDFLLVIGAEETKTEFENVKLLREARNDLN
                     RATNAEAANIAKTVNASMKTINNIIKIMDTIGLDQLSGDLQEIAQLRIQHPDYSIQQL
                     ADSLTVPITKSGVNHRLRKINKIADELTD"
BASE COUNT         1261 a          871 c         1160 g         1411 t
ORIGIN      
        1 gcaacagctt tgattgctcg gtctattggc gcaggagata gggaatctct tacctttcat
       61 gtccgtcaag ccttagtgct ttcagtaggt gtaggtatgc tatttggttt actatccctt
      121 gttttcggac ggcagatgct ggtcttaatg ggagcagaaa tggagagttt ggctggcgcc
      181 caggcatttt tttactgggt tggtggtttg acgatttttc aggccttgat gaccattttg
      241 ggaacaatcc tgcgggcatc tggggataca gtatctccta tgaaaatgag cttactgacc
      301 aatggtttta atgtggtctt ggactatttc ttgatttttg gtattgggtc ttggtcaggt
      361 ttggagattg tagggacagc cttgggaacc gtcttagctc gcttgcttgg tacagtcttg
      421 ctttaccgaa aggtgcaaca gactgacttg gcagttgttc tcaaacaaat gttccacttg
      481 ggaagtccta aggaaatggt ggttttgacc ctacctgcag cagccgaacg cttggttatg
      541 cggttgggac aggtgttcta tttcagtttg attgtggggc tggggacgac tgtctatgct
      601 tctcatatga ttgcaggtaa tatcgagagt tttacctata tgcctgccta tggtcttgcg
      661 acagcggcgg ccgttttgat cggtcaagcc ttaggaaagg gggacatcct cacagttaga
      721 cgggtcgcat ttctcagctc tgcctatgga gtagccatca tgtccctgct cggtaccatc
      781 ttatttttcg gaactccaag ttttgctctg ctatttacca aagacctaga agccgtccgt
      841 caagtcgtca cagccttacg tatcgatgct ttcaaccagc caggccttgc ggtttctttg
      901 atcatggctg gcgctctgca gggcttgggg gataccaagt cgccactcta ctccacaatg
      961 ataggaatgt gggggctgcg cgtctcaggt gttatcgtct taggtcagat gctcggttta
     1021 ggaattgccg gcgtctggct gtcgattttg attgacctgc tcttgcgagc gatttttctg
     1081 acttggaggt ttcatgtaaa aacaagaaaa ctggctgaat aggatagttt tttggtataa
     1141 gcaggtagtt ttagaagtag attatggatt gccttatttc atgttggtga tactatctca
     1201 attccttttt tataaaatag gtgtaggata gtagtaggtg agctgtttta tacagaactt
     1261 gtgctataat agatataaaa aattaaaagg agatagagat atgaaaacca ttcacacaga
     1321 taaggcacct gcagcaattg gcccatacgt tcaagggaag gttgttggaa atttcctatt
     1381 tgcctctggt caagttcctt tgtcacctga aactggtgaa gtggttggtg aaaccattca
     1441 ggagcagact gagcaagtct tgaaaaatat cgcagcaatt ttatcagaag caggaacaga
     1501 ctttgaccat gtggtgaaga cgacttgttt cctaaaagat atgaatgatt ttgtagcctt
     1561 taatgaagtt tataaaacag ctttctcagc agattttcca gctcgttcag cggtggaagt
     1621 tgcacgcttg ccacgtgatg ttaaggttga gattgaagtt attgcagtta ttgattagaa
     1681 gagaaaagga gggaatgtca tgtcggacaa actccattta gtaattgtga cagggatgtc
     1741 tggagctggt aagacggtag ccattcagtc ttttgaagac ttgggctatt ttaccattga
     1801 caatatgcca ccgaccctct tgccaaaatt tttagaattg attcgccaca gccaggataa
     1861 caataagatt gctcttgttg tagatatgcg gagtcgctcc ttcttttcgg agattcgaga
     1921 ggtgttggat gagattgagg gggcagagga tttagatttt aaagtcctat tcttggatgc
     1981 gacggatagt gaattggttg ctcgctataa agaaactcgt cgttcccatc ctttggcggc
     2041 tgatggtcgt gttttggacg ggattcagct agagcgtgaa cttttggctc ccttgaaaaa
     2101 tatgagtcaa aatgttattg atacgacaga gctgactcct cgcaatctgc gtaaggcaat
     2161 ctcggagcag tttgctagtc aagataatca gccgtcgttt cgcatagaag tgatgtcctt
     2221 tggtttcaaa tatggactac ctctggatgc agacttggtt tttgatgttc ggttcttacc
     2281 aaatccctac tacaagttgg agttgcgtaa tttgactggt ctggatgctc ctgtctttga
     2341 ctatgtcatg gagcatcaag aatcggaaga gttttatagt catttgctcg gcttgataga
     2401 accgattctt cctggttatc agaaggaagg aaaatcagtt ctaacgattg ccgttggatg
     2461 tacgggtggt cagcatagaa gcgtagcctt tgctaaacgt ttggcagacg atttagagaa
     2521 aaattggaat gttaatcgta gtcatcgtga taaggacaga cggaaggaga cggtcaaccg
     2581 ctcatgagaa aaccaaagat tacagttatc gggggcggga ctggtattcc agtcattttg
     2641 aagagtttgc gggataagga tgtagagatt accgctatcg tgacggtagc tgatgacgga
     2701 ggatcttcgg gggaaatccg tcaggctttg caggtgaccc ctccaggtga tttgcgtaac
     2761 gtcctattgg ctatgtcgga tatgcccaaa ctttatgaac aaattttcca gtatcgtttt
     2821 gcggattcag atggaccctt ggctggtcat ccactaggga atctcatcat tgctggtatt
     2881 tcagaaatgc agggttcaac ctacaatgcg atgaggttgt tgacgcgctt tttccatacg
     2941 acagggcgga tttatccatc tagcgaacag gccttgaccc ttcatgctat ttttacagat
     3001 gggaccgagg tagcaggtga gagcaagatt tccaagcata atgggatgat tgatcatgtc
     3061 tatgtgacaa attcttataa tgatgatgaa ccgaaagcca gtcgccaggt agttgaaacg
     3121 attatggaaa gtgacatgat tgtacttgga ccgggttctc tttttacctc aatcttgccg
     3181 aatctgatga tttctgacat tggcaaagcc ctaaaagaaa ccaaggctga agtgacctat
     3241 gtgtgcaaca ttatgaccca acggggagag actgagtttt tctcagatgc ggaccatgtg
     3301 gcggtcctca atgcacacct agctgaacaa ttcattgata cagttttggt caatattgag
     3361 ccagttccac aagagtatat gaatagcaat cagtttgatg aatacttggt acaggtaaaa
     3421 cacgattttg caggtttgca aaaacaggct aatcgtgtga tttcttcgaa atttcttcgt
     3481 ttggaaaatg gcggagcttt ccatgatggt gacttggtgg tagaagagtt gctcaagatt
     3541 ttgcaggtgc ggtcatgagt tttacagtac aagtaaagga agaattgctg cttcagtcta
     3601 gccaaaataa gagcgagcta tctgctatta tcaaattatc aggtagtttg ggcttagctt
     3661 catcaggttt gactctttct atcagcacgg aaaatgcaaa gattgcccgt cacatttatg
     3721 aactgttact ccatttctac cagattaagg ctgagattcg ccatcatcaa aaacctaatc
     3781 tcaaaaagaa tcgtgtgtat gcagttttga tagaagacgg cgtgaatgag atattgaacg
     3841 atttgcattt ggctgatagc ttctttggat tagagacggg gatttctccc ttggttttgg
     3901 aaaatgattc gtggagtcaa gcctatcttc gtggagcatt tttagcggca ggctctgtta
     3961 aggatcctga aaaaggaaaa taccagcttg aaatagcttc tgtctatagt gaccatgcca
     4021 atgacctggc caatctgatg cagaaatttc ttttggatgc caaagtcatt gagcgaagta
     4081 agggaaccat tacctacctg caacgtgcag aggacattat ggattttctt ttggtgattg
     4141 gagcagaaga gacaaagaca gaatttgaga atgttaaatt acttcgtgag gctcgcaatg
     4201 acctaaatcg agctacaaat gctgaagccg ctaatattgc aaagacggtc aatgctagta
     4261 tgaaaacaat caataatatt atcaaaatta tggatactat cggtctagat cagttgtcag
     4321 gtgatttgca ggagattgcc cagttgcgaa ttcagcatcc agactattcc attcagcaat
     4381 tggcggatag tctaacggta ccgatcacta aaagtggtgt caaccatcgt ttgcggaaaa
     4441 tcaataagat tgcggatgaa ttgactgact agataggtat acacagaatg ttatagacaa
     4501 tatgtaaaga cccccagttg agagttcgtg gatttacctg tacactagaa taaaaaaaca
     4561 atcaacttct aacaggaatt accacactca attggaggtc ttatatgttt cattttacca
     4621 cacttttcat cggaatggat gttcacaaag aaagtttttc actctgctat tatgatatga
     4681 tggcgaatca attcaaacat agc
//
LOCUS       DATUCG010000155         4699 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.157, whole genome shotgun sequence.
ACCESSION   DATUCG010000155 DATUCG010000000
VERSION     DATUCG010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4699
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.157"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(589..2133)
                     /locus_tag="VCA20_002183"
     CDS             complement(589..2133)
                     /locus_tag="VCA20_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEP1835917.1"
                     /translation="MSLQEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKTGNFAKSDWMDIEKQRGISVTSSVMQFDYAGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRNIPVFTFINKLDRDGREPLDLLQELEEVLG
                     IASYPMNWPIGMGKSFEGLYDLHNKRLELYRGDERFASLDEGDKLFGSNPFYAQVLDD
                     IELLAEAGNEFSEQAILDGDLTPVFFGSALTNFGVQTFLDTFLEFAPEPHGHKTTTGD
                     VIDPLNKDFSGFVFKIQANMDPRHRDRIAFVRVVSGEFERGMAVNLPRTGKGAKLSNV
                     TQFMAESRENVENAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAIQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMTPMGKKTVRWIKPEDLDERMSSSRNILAKDRFDQPVFLFENGFALRWFADKYPDV
                     ELEEKM"
     gene            2370..>4699
                     /locus_tag="VCA20_002184"
     CDS             2370..>4699
                     /locus_tag="VCA20_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEP1835918.1"
                     /translation="MNKQIKGLSTKEVQERIQNQQTNHFKTKTSASNWEIFRRNVFTS
                     FNALNFAIFLALLAVQAWSNLFFFGVIVLNAVSGMLTEWRARRMIDKLNLMNKDFIRV
                     IRDSETISIAPEDIVLDDVLLLSTGEQVPSDAIVLEGIAEANEAMLTGESDLIVKNTG
                     TELLSGSYLVSGQIYAKVIHVGAENYANKLMLEAKTHKPIVSRILYNMDKIAKFTGKI
                     IIPFGLALFLEAFFIKLLPLKDSVITSSTALLGMLPKGIALLTITSLLTAVIKLGMKN
                     VLVQEMYSVETLARVDVLCLDKTGTITQGKMTVENLLPLTNHYSLDTIQQILAAYIQT
                     SEDTNSTAQAIRKEYGDLEHHYKASHIIPFSSDRKWGAMTIENIGHIFLGAPEMLLTQ
                     NPPSVSEAQARGSRVLILALSQQSLPSSQNQLPENIEPLALLEIADPIREDAAETLAY
                     LRSQEVTLKIISGDNPVTVSHIAREAGFADYDSYIDCSKVDDEELIARAETTAIFGRV
                     SPHQKKLLIQTLKAQGHTTAMTGDGVNDILALREADCSIVMAEGDPATRQIANLVLLD
                     SEFRDIPEILFEGRRVVNNISHIAPIFLIKTIYSFLLGLICIASIALGKAEYLLVFPF
                     IQVQMTLIGQFVEGFPPFILTFERNIRPVEKHFLRKSLLLALPNALMVVLSVLIFHLM
                     QVFGYLNLHDMQTLSYYVLGSTGLLAVIRACLPLTKARLALIIYSVFGFFISSHFLQG
                     LIEIHPLNSHTLPIYSGLMLIFIPVFFWISYKQGAF"
BASE COUNT         1312 a         1103 c          909 g         1375 t
ORIGIN      
        1 ttccaaagaa agataaaaag caaggcacct gtaagcttca cttatagagt aacataatag
       61 ttgaaaaata gggttaaaaa acctcctttt tgtgcgccat aaatacaaaa actattcccg
      121 ttttgtcatc aattgctcag acgggaatag tttctttagc tgcttaaatg cttaacttaa
      181 tgacattggg attaaccgag ccttattcct ctattatttc gagtagatag tgattttcaa
      241 agctgattac tgactttacg caaagaaact caagaataaa actaggcaag gagctgaaga
      301 ttgaactaga ggttcatcga agcaacttaa cgaagtttta gcttgatttt caaagctgaa
      361 cagctgtaag ctgataccat cagctctcat gtcccacctt tcacctcgct tgacatctct
      421 aggctcaggc tgaaacagtt ccccgaactg tttcactcca ctgaacctcg atgcagtagg
      481 cgtcacaaca aatcactctt cgcattgctc gagttctttg gacgcccttg cgagggaaag
      541 acgacgaaat caaatcaact ttgttgatta ctgatttctg tctttccgct acatcttctc
      601 ttccaactcc acatcaggat atttgtccgc gaaccagcgg agggcgaagc cgttttcaaa
      661 aaggaaaact ggttggtcaa agcggtcttt ggctaggatg ttacggcttg aggacatgcg
      721 ttcatccagg tcttctggct tgatccagcg gactgtcttt ttacccattg gagtcatgac
      781 tacttcggca ttgtactcgc cttccatgcg gtgtttgaaa acttcaaact ggagctgacc
      841 tacggccccc agcatatact cgcctgtctg gtagttcttg tagagctgga tagctccttc
      901 ttgcaccagt tgctcaattc ccttgtggaa ggatttttgt ttcatgacgt ttttagcaga
      961 aactttcatg aaaatttcag gggtgaaggt cggtagcggt tcgaattcaa acttgttctt
     1021 acctacggtc aaggtatctc ctacctgata ggtccctgta tcgtaaaccc cgataatatc
     1081 acccgctact gcattttcca cattctcacg ggactcggcc atgaactgcg tcacgttgga
     1141 caacttggct cccttacctg tacgaggcag gttaaccgcc ataccgcgtt caaattcgcc
     1201 cgaaaccaca cggacaaagg cgatacggtc gcggtgacga gggtccatgt tggcttggat
     1261 tttgaagaca aagcctgaaa agtctttgtt gagcgggtca atgacatccc ctgtcgttgt
     1321 cttgtgacca tgtggctctg gagcaaattc caagaaggtg tcaaggaagg tctgcacacc
     1381 aaagtttgtc aaggctgagc cgaagaaaac aggtgtcaaa tcgccgtcca aaatcgcctg
     1441 ctctgaaaat tcattcccag cttccgccag aagttcaata tcatccaaaa cctgtgcata
     1501 aaaggggtta gaaccaaaga gcttgtcgcc ttcgtccaag ctggcaaaac gctcatcacc
     1561 tcgatagagt tctaaacgtt tgttatggag gtcatagagt ccctcgaagg atttccccat
     1621 accaatcggc cagttcattg gatagctggc aatgcccaag acttcttcca actcttgcag
     1681 caaatcaagt ggctcacggc cgtcacggtc caacttgttg ataaaggtga aaacagggat
     1741 gttgcggtgc ttaacaacct caaagagctt tttggtctga gcctcgatac ccttggcaga
     1801 gtccaccacc atgacagccg catcgaccgc catcaaggtc cgataagtgt cttctgagaa
     1861 gtcctcgtgc cctggtgtat ctaggatatt gacccgcttg cctgcatagt caaactgcat
     1921 gacagatgag gtaacagaga taccacgttg tttctcaata tccatccagt cagacttagc
     1981 aaagttacca gtctttttcc ccttgactgt acctgcttca cgaatctcac ccccaaagta
     2041 gagcaactgc tcggtaatag tggtcttacc cgcgtccggg tgagagataa tcgcaaaggt
     2101 gcggcgtttc ttgatttctt cttgtagtga cataatgttt ccttttcatt ctttgtattt
     2161 tgattcatag cagagctgag ctcttatcct attcgattta cattggattc atccttattc
     2221 tttactagac aactctctta ttatatcgga aataagggaa tttttcaaac tattgaagaa
     2281 taggtctaca gacctatact attgtattat aaatatgtta caataagtat gagatgtatt
     2341 ctatatacta gaaaaagaaa gataaaataa tgaacaaaca aataaaaggt ttatcaacca
     2401 aggaagtaca agaacgtatt caaaaccagc aaaccaatca ttttaagaca aagaccagtg
     2461 ccagtaattg ggaaatcttc agacgaaatg tgtttacatc ttttaacgca ctaaattttg
     2521 ccattttctt agccttacta gctgttcagg catggtccaa ccttttcttc ttcggagtta
     2581 ttgtcctcaa cgctgtttca ggaatgctga cagaatggcg agcacgacga atgattgaca
     2641 aactcaacct gatgaacaag gattttatcc gagttattcg cgacagcgag acaatctcta
     2701 ttgcaccaga agatattgtc ttagatgatg ttctcttgct atctactggc gaacaagttc
     2761 ctagtgatgc tattgtttta gaaggtatag ccgaggcaaa cgaggccatg ttaaccggtg
     2821 aaagcgactt gattgtgaaa aataccggga ctgaattatt gtctggtagc tatctagtta
     2881 gcggacagat ttacgcaaaa gtcatccatg ttggcgcaga aaactatgcc aataaactca
     2941 tgcttgaggc gaaaactcac aaacctatcg tctctcgtat tctttacaat atggataaaa
     3001 ttgctaaatt tacaggaaaa attatcattc cattcggact tgctcttttt ctagaagctt
     3061 ttttcatcaa acttcttccc ttaaaagact cagttattac cagctctact gccctactag
     3121 gtatgttacc aaagggaatt gctctgctga ccatcacttc tctcttgact gccgttatca
     3181 aactgggaat gaaaaatgtc ctcgtacaag aaatgtattc cgtcgaaaca cttgcccgag
     3241 ttgatgttct ttgtttggat aagaccggaa cgattactca ggggaaaatg accgtcgaaa
     3301 acctcctccc cttaaccaac cattattcac ttgatactat ccaacagata ctcgctgcct
     3361 atatccagac aagtgaggat actaattcaa cagctcaggc cattcgaaaa gaatatggtg
     3421 atttagagca ccactataaa gctagtcata tcatcccgtt ttctagtgat agaaaatggg
     3481 gagcaatgac aatagaaaac attggtcaca tttttctagg ggcaccagaa atgttactaa
     3541 cacaaaatcc tccttctgtt tctgaggcac aggctcgtgg ttcccgtgtc ctgattcttg
     3601 cgcttagcca acagtccctt ccttcttctc aaaatcagct acctgagaat attgaacctc
     3661 tagctctatt agaaattgct gaccctatcc gagaagacgc cgcagaaaca ctggcctatc
     3721 ttcgctcgca agaagtgaca ctaaaaataa tctctggtga caatcctgtg acggtctcac
     3781 acattgccag ggaagctgga tttgctgact acgatagcta tatcgattgt tccaaagttg
     3841 acgacgaaga actaattgcc agagctgaaa caaccgccat tttcggtcgc gtatccccac
     3901 atcagaaaaa attgctcatc caaaccctca aagcacaggg acatactact gcaatgaccg
     3961 gtgatggggt caatgatatt cttgcactac gcgaagccga ttgttccatt gttatggcag
     4021 aaggtgatcc cgctaccaga caaatcgcta atctagtcct cctagattct gaatttcgcg
     4081 atattcctga gattctcttt gagggacgac gcgttgttaa taacatctcc cacattgctc
     4141 ccatctttct tatcaaaaca atctactctt tcttacttgg ccttatctgt atcgctagta
     4201 ttgccctagg taaagcagaa tatttgttag ttttcccatt tattcaagta caaatgaccc
     4261 tcattggtca atttgtcgaa ggttttcctc cttttatcct aacttttgaa cgaaatatcc
     4321 gccctgtaga aaaacatttt ctcagaaaat ctcttctctt agccctacca aatgctctca
     4381 tggttgttct cagtgtcctt attttccatt taatgcaagt atttggatac ttaaacttgc
     4441 acgacatgca aaccctctct tattatgttc ttggttcaac aggtctactg gctgtcattc
     4501 gtgcctgttt gccactgaca aaagctcgac tggctcttat tatttattcc gttttcggtt
     4561 tcttcatcag ctcccatttc ctacaaggtc tgattgaaat ccacccccta aacagtcata
     4621 cattgccaat ctattctgga ttaatgctca tttttatccc tgtctttttc tggattagct
     4681 acaaacaagg cgcatttaa
//
LOCUS       DATUCG010000156         4688 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.158, whole genome shotgun sequence.
ACCESSION   DATUCG010000156 DATUCG010000000
VERSION     DATUCG010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4688
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.158"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            357..1292
                     /gene="mvk"
                     /locus_tag="VCA20_002185"
     CDS             357..1292
                     /gene="mvk"
                     /locus_tag="VCA20_002185"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEP1835919.1"
                     /translation="MSVVGKANGKIILMGEHAVVYGQPAIAMPFSAVEIIAQVTAQGE
                     ALTVACDFYKGLVHKMPKIWESLKHAIRFSLYRIGAPTDPAIHIEISSTIPAERGMGS
                     SAAVAVAVARALFAYYKKELTDSELWDIVQSSEKIAHGNPSGIDAATTSGKSPVFFIK
                     HQPIEPFELKLHAHLVVADTGVTGNTLEAISDVAALLEKKPEAIKLVEELGNLTRQAK
                     EDLATDQAELLGSRMDQAHALLQKLGVSDPSLDKLVSLAQENGALGAKLTGGGRGGCM
                     IALARTAQDAQKLTHILDQAGARQTWIQYLGETND"
     gene            1285..2310
                     /gene="mvaD"
                     /locus_tag="VCA20_002186"
     CDS             1285..2310
                     /gene="mvaD"
                     /locus_tag="VCA20_002186"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEP1835920.1"
                     /translation="MTKQTGTARAHTNIALIKYWGKRDKELFLPMNSSLSLTLDAFYT
                     DTKVVFDPELTADEFYLNGILQKEKEILKISRFLDLFCEYIGERAFARVESLNFVPTA
                     AGLASSASAFAALALATATALDLDLSPATLSTLARRGSGSSTRSLFGGFVEWDMGTGS
                     EDSMAHPIDDADWDIGMVVLAVNTGPKKIASREGMDHTVATSPFYTAWVETAKQDLVD
                     IKAAIASRDFEKLGQITEHNGMKMHATTLSANPPFTYWSADSLVAQEAVRQVREATGL
                     SAYMTMDAGPNVKVLCRASQMDELVEELSAVFPREKIITSKPGPAAYVLSEKDWQASQ
                     TAFEKGI"
     gene            2312..3391
                     /locus_tag="VCA20_002187"
     CDS             2312..3391
                     /locus_tag="VCA20_002187"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEP1835921.1"
                     /translation="MKVQVKIPGKLFLAGEYAVVEAGYPAVIAAVGQYLTVTIETSDQ
                     GSLHSSQKADLYLTWERKEGAVRIQGSHPYALIESAMQVTEAYLTAKGYACHGTYSLS
                     VQSDLDDQASGAKYGLGSSGAVTVATVKALLTYYGHRPDALLTYKLAALAQTKLGMTG
                     SFGDLAASSFGGLIAYHSLDRSWLLGKMAELSLLDLVESDWKGLSISPIQLPQGLDLL
                     VGWTGSAASTDRLVSQMESQKSQAEKEQIHSQFLADSKTCVEQLIVACQTNDIVSARQ
                     AITQNRKLLQDFARGMGLVIETPQLSQLCDLAQTYGAVAKSSGAGGGDCGICLVDSKE
                     QKVAIEKAWQQAGILPLQLKITSRE"
     gene            3402..4499
                     /locus_tag="VCA20_002188"
     CDS             3402..4499
                     /locus_tag="VCA20_002188"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEP1835922.1"
                     /translation="MFYLGEFGLDRKDQHVGLANQQYSATPAKDFTETLFVHHSLPQT
                     KVDEVDISTSVAGLDFAFPFFINAMTGGSKKTCEINRLLGIMGHFGKIALASGSVSAA
                     IKDPSVAETFSVMRRENPYGIIFANLGAHHSVENAKRAVDLLEANAIQIHVNAPQEIV
                     MPEGDRDFTMWLKNIETLVREMEVPVIVKEVGFGMSRETVAQLASVGVKTIDVSGTGG
                     TDFAKIENARRTFNDYTYLEGWGQSTVTSLVEAMSVSEEVRPSLIASGGIKTPLDIVK
                     SLALGADLVGMSNHFLQYIKDGKGHRFDDGLQAINTYQWQMAEIMTMLGAKNIAELRQ
                     KDLILAPNVQNWCEARGIDWKAYARRSANVS"
BASE COUNT         1273 a         1045 c         1168 g         1202 t
ORIGIN      
        1 tatactatat tcaagctaga aatagcgaat ttctttttca tataatctcc agagaacagt
       61 cgaccgcggt cggctgtttt cgtgtcaagt tttaagaaaa taagtatgag aaattgcttt
      121 agaaaaatct aagaaatctt tcagtttcgc ttaagtttgt ggatgtatac tatattcaag
      181 ctagaaatag cgaatttctt ttcacctaaa ggtgtcccta tctctaagtc gcaagcgaca
      241 aagagctagg tatcatataa tctcctagag aacagtcggc cgcggccggc tgttttcgtg
      301 tgttctattc ttatgtaaaa atggtataat agtagtgatt gtataaggag aaaagtatgt
      361 cggtagtagg aaaagcaaat ggaaaaatca ttttgatggg agagcatgcg gtggtctatg
      421 ggcaaccagc aattgctatg cctttttctg ctgttgaaat tattgctcag gtaactgctc
      481 aaggggaagc gctgactgtt gcttgtgact tctataaagg cttagtgcac aagatgccaa
      541 aaatctggga aagcctcaag catgccatcc gtttctcgct ctatcgtatc ggagcaccga
      601 cagacccagc tattcatatc gagattagtt ccaccattcc tgccgagcgc ggcatgggct
      661 ccagtgcagc cgtggcagtt gcagtagcac gcgccctctt tgcctactat aaaaaagaac
      721 tgaccgacag cgaactctgg gacatcgtcc agtcttcaga aaaaattgcc cacggcaatc
      781 catcaggcat agatgcggcg accaccagcg gcaagtcccc cgtcttcttt atcaagcacc
      841 agcccatcga accttttgag ctgaaactcc atgcccattt ggtagtggct gacacaggcg
      901 tgacgggaaa caccttggag gccatttcag acgtggcggc tttattggag aaaaagccag
      961 aggctatcaa gctagtagaa gaactgggga acttgacccg acaggccaag gaagatttag
     1021 ccacagacca agcagagctt ctaggtagca gaatggacca agctcatgcc ctcctgcaaa
     1081 aattaggcgt gtctgatcct agcctagata aactcgtcag ccttgcccaa gaaaatggag
     1141 ccctcggagc caaattaact ggtggcggcc gaggaggctg catgattgcc ctggcaagaa
     1201 cagctcagga tgcccaaaaa cttacacaca tccttgacca agcaggtgct cgccaaacct
     1261 ggatacagta cttaggagaa accaatgact aaacagacag gcactgcccg tgcccatacc
     1321 aatattgcct tgattaaata ctggggaaaa cgcgataagg aattattcct acctatgaat
     1381 tctagcctat ccttgaccct tgacgcattt tatacggata ccaaggttgt ttttgaccca
     1441 gaattaactg ccgatgagtt ctatctcaac ggaattttac aaaaagaaaa agaaatttta
     1501 aaaatttctc gatttttgga tttgttttgc gaatatatag gtgaaagggc atttgcccga
     1561 gtggaaagtc taaattttgt tccgactgcg gctggtttgg caagctcagc atcagctttc
     1621 gcagcccttg cgcttgcaac cgcaactgcc ttggatttag atttgtcacc agctacccta
     1681 tcaacccttg ctcgcagggg ttctggctct agcacccgta gcctattcgg tggatttgtt
     1741 gagtgggata tgggaactgg ttcggaagat tctatggctc accccattga cgatgcggat
     1801 tgggatatcg gcatggtcgt cttggcggtc aatactggac cgaaaaagat tgctagtcga
     1861 gagggaatgg accatacggt tgccacctcg ccattttaca cagcctgggt agaaaccgct
     1921 aagcaagact tggtggacat caaggcagct attgccagcc gtgattttga aaaacttggt
     1981 cagattacag agcacaatgg catgaaaatg catgcaacaa cgctctctgc caatccaccc
     2041 tttacctatt ggtcagccga tagcctagtg gctcaagagg ctgttcgtca ggttcgtgaa
     2101 gcaactggct tatcagccta catgaccatg gatgcgggac caaatgtcaa ggtcctctgc
     2161 cgagcaagtc agatggatga attggttgag gagctatcag cagttttccc aagagaaaaa
     2221 atcatcacta gtaagcctgg ccctgccgct tatgttctca gtgagaagga ttggcaggca
     2281 tcacaaacag catttgaaaa gggaatctag catgaaagtg caagtaaaga taccaggaaa
     2341 actctttctg gcaggggaat atgcagttgt tgaagctggc tatcctgcgg tgattgcggc
     2401 ggttggccag tacctaaccg tcaccattga aacaagtgac caaggcagtc ttcattctag
     2461 tcaaaaggca gacctttatc tgacctggga gcgtaaggaa ggtgccgttc gtattcaagg
     2521 tagccatccc tatgccttga ttgaatcagc tatgcaggtg acagaagcct atctgacggc
     2581 caagggctat gcttgtcatg gaacctatag cctatctgta cagtccgacc tagatgacca
     2641 agcctctggt gccaagtacg gtctggggtc gtcaggggca gtaacggttg cgacggtcaa
     2701 ggccctgttg acctactacg gtcaccgacc agatgccctc ctgacctaca agctggcagc
     2761 cttggcccag accaagctag gcatgacagg ttccttcgga gatttggcgg cttccagttt
     2821 tggtggcttg atcgcctatc attccctgga ccgttcttgg cttttaggga aaatggcaga
     2881 gctgtccctg ctggatttgg tggaaagtga ttggaaaggt ttgtctatca gccctatcca
     2941 gttaccccaa ggtttagacc tcttggtggg atggacagga tcggcggctt cgacggacag
     3001 gctagtttct cagatggaaa gccaaaaaag tcaggcagaa aaagagcaaa ttcacagcca
     3061 gtttctagcc gactccaaga cctgtgtaga acagttgata gtagcctgtc agacaaatga
     3121 catagtatca gctagacagg ctattactca aaaccgcaag ctcttacaag actttgcgag
     3181 aggaatgggt ttggtcattg aaacgcctca actgagtcaa ctctgtgact tggcccagac
     3241 ctatggggcg gtggccaaat catctggtgc aggcggtggt gactgcggca tttgcttggt
     3301 agacagtaag gaacaaaaag tagcgattga gaaggcttgg caacaagccg gcattcttcc
     3361 actccaacta aagatcacaa gtagagaata aggagaaccc tatgttttat ctaggagaat
     3421 ttggtttaga ccgcaaggat caacacgttg gcttggccaa ccagcaatat agtgccacac
     3481 cggccaagga ctttacggag accttgtttg tccatcattc cctgcctcaa accaaggtgg
     3541 atgaagtgga tatttcgacc agcgtagctg gtttggactt tgcctttcct ttcttcatca
     3601 atgccatgac tggtggaagc aagaagacct gcgaaatcaa ccgtctgttg ggaattatgg
     3661 gacattttgg gaagattgct ctggcttctg gttcggtcag tgcggcgatc aaggatccat
     3721 ctgttgcgga aaccttctcc gtgatgcgtc gtgaaaatcc ttacgggatt atctttgcca
     3781 acctaggtgc ccatcacagt gttgagaatg ccaagcgggc agtggatttg ttggaggcaa
     3841 atgccattca gatccatgtc aatgcccccc aggagattgt catgcctgag ggagaccgtg
     3901 actttactat gtggttgaaa aatattgaaa cccttgtacg tgagatggaa gtgcctgtca
     3961 tcgtcaagga agttggtttt ggcatgagcc gtgaaactgt cgcccagttg gcttctgtcg
     4021 gtgtcaagac cattgatgtg tctggtactg gggggacgga tttcgccaag attgaaaatg
     4081 cccgccgtac cttcaatgat tatacttatc tagaaggttg ggggcagtct accgtgactt
     4141 ccttggtaga agccatgtct gtttctgaag aagttcgccc aagcctgatt gcatctggtg
     4201 gtattaagac gccactagat attgttaaat cattggcttt gggtgcagac cttgtcggta
     4261 tgtccaacca tttcctccag tatatcaagg atggaaaagg gcatcgtttt gacgacggct
     4321 tgcaggctat taatacttat caatggcaga tggctgaaat catgaccatg ctgggtgcta
     4381 agaatattgc ggaactacgt cagaaagatt tgatcttggc tccaaatgta caaaactggt
     4441 gcgaggcgcg tgggattgac tggaaagcct atgcccgtcg gtcagcgaat gtgagttaaa
     4501 gagagatata gtggattgaa ttaggaaaaa gacaatttta agttatagtg ttaataaccc
     4561 actcctaaaa taactttaga gttctcaaat tgacaaactt atatatgtcc tgcttctaat
     4621 tcactcgact atagattgat gccttcggtt ctcaacaatg tagaaaaata aaaaagccag
     4681 gtccattt
//
LOCUS       DATUCG010000157         4669 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.159, whole genome shotgun sequence.
ACCESSION   DATUCG010000157 DATUCG010000000
VERSION     DATUCG010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4669
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.159"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            26..1636
                     /locus_tag="VCA20_002189"
                     /pseudo
     CDS             26..1636
                     /locus_tag="VCA20_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            1670..4296
                     /locus_tag="VCA20_002190"
                     /pseudo
     CDS             1670..4296
                     /locus_tag="VCA20_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386153.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase/lanthionine synthetase C family
                     protein"
     gene            4323..>4669
                     /locus_tag="VCA20_002191"
     CDS             4323..>4669
                     /locus_tag="VCA20_002191"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835923.1"
                     /translation="MIDRNKEFLKTVNGRGEYKNNILDALNSSADSNSYIFRRGNHWY
                     RLENWDLPNSGIIREWKASGDDCIPKADYSDSFNIKNSKTILDVKKINYQHMLGDISQ
                     ITFTDDGKIIMVL"
BASE COUNT         1723 a          565 c          821 g         1560 t
ORIGIN      
        1 attataaagc gcaaaaggag aaaatatgac atttagagaa ttatggagta ttgtaccaaa
       61 agttaagcta tttgcattaa taatttttac tataatagct ggttttgatg gagtggttat
      121 gtcacaagtt atttcgtcag ttacaaaatt tggatcaaac tctacaccaa aagatatatt
      181 ttcattgctt atttacggat tattctcata tcttgtagtc caaattgcaa atacattttc
      241 attattttta aataacaaca taattaagta cttgaatata atttttaaga agaaggtgat
      301 agatacaatt tactcaatca gaggaaatag cacagacatt gaagattcta tcgctacact
      361 aactgtagat tttaaaataa ttgaggaaca atatttttcg gtcatattta aaatcatcta
      421 ttttatatta atgggaataa tttctcttat atatttagtt tatcttagtc cattaatatc
      481 agttttattc ataggatgtt catttttacc agtgttgcca tcaattattt ttggtaaaaa
      541 attggcgaaa gtaacagaag aatatactgg aaaaacttcg attttcattg aaaatttaaa
      601 agatatgtca gaaggattcc aagaaatttt tagctataac gcatttgata tatttcagaa
      661 ttgtattaat ttttttacaa atgaaatgga agaaagtgca gagcgtttaa aaaataaaca
      721 tgcttacgta agtttggtct cggctctctt atcctggttg ggttatttaa taccaattag
      781 tgtagcactt tatttggtga ttaaggggag tatcgatgca ggaattgtaa tcgcactttt
      841 tttagcaagt gattgagtta tttcaccatt aagaaatatt tcagaatatc ttcgtatgct
      901 ccaatctact gaaagtgtaa gaagaaaaat taagaagata atttcctacg ataaaaatat
      961 aaatattact tttcataatg tggacaaacc taacataatt tttaatgatg tagtatttgg
     1021 atttgatcat attattatta ataacgaaag ctttattatc ccatatggaa gtaaagtatt
     1081 ggtcaaaggt ccgtcaggaa gtggtaagac tacattatta aatctaattc aaggaagcat
     1141 agttccacag aaaggaaaag tattagtaaa taatattagg acttttgaag tattgtcaaa
     1201 tccaagtatt ataacaagaa tttatcaaaa tattcactat tttaacatga ctttgcgtga
     1261 aaatatcaca atgggaaaat ctgatatatc cgataaacag ctatattttc tacttaataa
     1321 gtttggttta ttaaaggaat taggttcaga ttgtttgaat aaaaatttaa aaggtcaatt
     1381 atctggtggg caaattcaac gtattgcaat tatcagagct cttattcata acggaaaaat
     1441 actattgata gatgaagcga ctaattcagt agatttagaa acttcttcta tgattagaac
     1501 atttttttct gaatttgagg ggactgttat agaggtagct cacaattatc gaaaggaatt
     1561 ggatggatac tatacacata taatagaatt caaaggtagt actatccgct tatcggaatt
     1621 aaatacgaaa aaataacaaa tagttaataa tattatgagg tgaaatatta tgcaatatga
     1681 gtttaattta gataatccct tgttttttat taattcttca gattcaattg atacatctaa
     1741 aaagtattct gtggaattgc cgtatgactg gcacgaagtt gtcaataacg ccgagtggac
     1801 cactcagtat ccacttaata aaagtattga aaggcaagga tggaaaatcc atattagttc
     1861 aaactttgtg gattctcatg aaatacttga gattgttgct caaatttgtc acgatttttc
     1921 agtaactttt aaatatttaa gtacggaaaa aagatttgta ttaagaaata gtaaaatttc
     1981 aaatagagga cattcaggta agttcataac ttgctatcct agtatggaaa tacttgaaaa
     2041 tttcttaaat actttggagc aaaaactgaa aaacttttct ggcccttata ttttaagtga
     2101 taaaagatgg aaaaaggctc caatttacct aaggtatggt gtttttagag atacgatatc
     2161 tgaacaagag gaaaatctta aaatagatga gttattaatt tcaggtaaga taatcaaaga
     2221 tctgagagaa gcacgatttg ttgttccaga gggaatagaa ttacctattt tcttaaaaaa
     2281 atggttagaa aatactgaac agtctgactc acaaagatta ccttttgaaa taaaatctgt
     2341 cattcgcttt tcaaatagtg gtgggattta tagagcttat ttaaagaata tagataaaga
     2401 tgtgatttta agagaagtta gaccatatac tggtttggat tttgatggaa agtattcgtc
     2461 agataggcta attacagaag agacagcttt aagaaagcta aaagaaataa agaaaattcc
     2521 tgatgtttat tggagaggaa tagtctggga acatgaatat ttagccgtgg agagaatgaa
     2581 agggatacca cttaatagat gggtaacaaa aaactatcca atatacgatt ttgtagcaac
     2641 agataaaagt tatttatcta gggttaataa aattttgtct gagttgattt ctttggtaaa
     2701 gtgctcacat tcaaaaaatg tatttcatca agacattcat cttgcgaata tattaataga
     2761 tgagaaagat aatgtatcaa taatagattg ggaacaagct cgatttgaca atactcaaaa
     2821 gattgaacag caagcagctg ctttaggatt tggttcatgg attaaagatt atccaagtca
     2881 aatagattgg tttggagtaa aacaagttgc tcattatctc tattttccat tattagaaca
     2941 atctgcattt gtttatggat atgaacagca attattgaaa catgcaaagg aattatttac
     3001 ccgtttatct tttaaaaaag gagatattga agctgtagaa aagatactta aatacttgaa
     3061 taataaagtt gctccagtga aaaatctaac tgcaaaaaaa ttgtgaaacc attcaatgag
     3121 tctatagaaa tatttgataa atcagacttg ttagactatt ataataaact tatccgtgga
     3181 ttatacgttg cgtataatga atggaaacag actaccaaag taaataggag gtttcctgtt
     3241 cattactatg gtttagatat aaaccaaggg attgcatttt ctgatttagg tatcctttgg
     3301 agttatgaag aattagctaa attacttcag actgagactg ttactgatga atttagccag
     3361 ttaaaaaaag cagtaattca aagtgcaatc actagtttcg atgaacgcaa aatttcaccg
     3421 ggattatttg acggagttat aggttcaatt tggattattt ataaattagg agagacagat
     3481 ttagcaaaaa aattatttgc ttcacatttt gattcattat tcaaaatgtg tggtagtaaa
     3541 aatttatata gtggagaagc aggcatactt ttggtaggaa tttacttgct ttcaaatggt
     3601 cttgaagatg aatttatagc caataaatta actgaaaggc ttgagttatt ttcaaaagat
     3661 tataagcaga atccaaagtc cttttgtttt gtaggaaaaa gtcaaacaca aagtaatgat
     3721 ccttaccaaa atacatcagg tttattatat ggtcatttag gattatcctg gttattcgga
     3781 gaagcatata gatttacaaa aaatgaactt tacaaggact gtttgaatat ttcaattcta
     3841 acagaactta aggcatatga tttagataaa atgggctcat tacagtatca tcaaggaaat
     3901 aggctacttc catatttatc tactggaagt gcagggatgc ttttattaat ttctagaaat
     3961 aaacaattcc taaacaatga cattatggag gttaaagcga agcttgaaaa ggctgtaagt
     4021 tcaaattttt gtgtatttcc agggcttttt aatggattat gtggtctcga attgtcgagg
     4081 agactctacg atgaaaacaa tgattcaatc atgaatcaag tggagcttct aaaaaaactg
     4141 tcaatatact ttagttcgat tgaacgtggt ctagtcattg caggtgatag cggactaaaa
     4201 attacaacag atattgcctc ggggtttggt gggatagcac tatctttaat ctcattacaa
     4261 aataatcgat tagaattatt accgcatatt acttaataaa aatatatagg caggtgacaa
     4321 gtatgattga tagaaataaa gaatttttaa aaaccgttaa tggtagaggt gagtataaaa
     4381 ataatatttt agacgcattg aactcttcag cagattcgaa ttcatacatt tttagaagag
     4441 ggaatcattg gtatcgtttg gaaaattggg atttaccaaa ctcggggatt atcagagagt
     4501 ggaaggcaag tggagatgat tgtattccaa aagctgatta ttcagactct ttcaatataa
     4561 agaattcaaa aactatttta gatgttaaaa aaataaatta tcaacatatg ttaggtgaca
     4621 ttagtcagat tacatttacc gatgatggta aaataataat ggtgttatt
//
LOCUS       DATUCG010000158         4584 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.160, whole genome shotgun sequence.
ACCESSION   DATUCG010000158 DATUCG010000000
VERSION     DATUCG010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4584
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.160"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            275..970
                     /locus_tag="VCA20_002192"
     CDS             275..970
                     /locus_tag="VCA20_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEP1835924.1"
                     /translation="MRKVQFFLLIESMIFTMAAFDVVANEVSRTLLLFSALLLVIWYF
                     LGRKVNSVLLVSSLSLLFLVFVLNPFFIIGIMLLVVYMLINFFSRYEKRNQYTHIVFS
                     DYSLQVQKEKNRWFGNHDHSQDRFGFEDINIVRLFGNDVVDLDKTVLVGRDNIVVIRK
                     TFGRTKIIVPIDVEVALSATSIYGRVSFLGESYWDLRNESIAVNSPDYQESHKRVKVV
                     TNNILGDVEVVRV"
     gene            967..1983
                     /locus_tag="VCA20_002193"
     CDS             967..1983
                     /locus_tag="VCA20_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEP1835925.1"
                     /translation="MRKRTYLLLAWFACLIVFVVIASTLPLLNYSLLDVTLWISTEQL
                     VFTLILLVIVLTLFLAVMVQTVSLASTQVMRTKLQAILKNKSIRTDTESDQLLLQLSD
                     KVRNLTRQVQLVDNQDLVKKEEIVEGERKRIARDLHDTVSQELFATSMILSGLSSNLS
                     TLPQETLQEQLMLVKDMIESAQRDLRILLLHLRPSELESKTLVEGFELILREVSDKSN
                     IIVHFQHEVEELPKLIEEHLFRIAQEIISNTLRHAKAKHLDVYLLQKETELQLKMTDD
                     GIGFQQGADDELSYGLQNIRERVEDMAGTIKIRTAPNKGVAIDIRIPLLKGKEDEYDS
                     SDAG"
     gene            1955..2596
                     /locus_tag="VCA20_002194"
     CDS             1955..2596
                     /locus_tag="VCA20_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEP1835926.1"
                     /translation="MNTIRVMLVDDHEMVRLGLKSYLNLQPDVEVVAEASDGEEGLAK
                     ALEVKPDVVVMDLVMPKMTGVEATLALLKEWPQAQIVILTSYLDNEKIYPVLEAGARG
                     YMLKTSSADEILVAIRKVALGEYAIETEVEKKVEHHKRHPDLHDDLTAREREILTLLA
                     KGYDNQRIADESFISLKTVKTHVSNILSKLAVSDRTQAVIYAFQHGLVAQEDQ"
     gene            2646..4046
                     /locus_tag="VCA20_002195"
     CDS             2646..4046
                     /locus_tag="VCA20_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEP1835927.1"
                     /translation="MDAKLRYKAKKLKIVFFDIDDTLRVKNTGYIPESIQQVFKSLKE
                     KGILTGIASGRTPYGLVPEIKALKPDFFAMINGSYVEDAKGQVVYHQPMPQNLVESVL
                     NWAKEIGIEYGMLGSQKGTLSARTDRISQVIDLIYEGLETNPTFYKENDIYQLLTFEK
                     DGHEVELPEELQAELRSVRWDAISSDIVLKGSSKATGVAKVVEKLGLKPENVLVFGDG
                     LNDIELFDYAGISIAMGHSHPELQKHADYITKKVEEDGIFDALEKLGMVEKEKYFPQL
                     DLENVTGPVAHIKTNHGKLTVKLFPEIAPKTVANFVALSKDGYYDGIIFHRIIKDFMI
                     QGGDPTGTGMGGESIYGTAFEDEFSMEAFNLRGALSMANAGPNTNGSQFFIVQNQNFP
                     YNAKELERGGWPKEVAEAYVKNGGTPHLDQRHTVFGHLVDEESFVVLDAIAAVATDSA
                     DRPHEDVVIETIEIED"
     gene            4048..4419
                     /locus_tag="VCA20_002196"
     CDS             4048..4419
                     /locus_tag="VCA20_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEP1835928.1"
                     /translation="MKIGDKLKGTVTGIQPYGAFVQLENGTTGLIHISEIKTGFVDNI
                     YDHLKLGQEVLVQVVDFDEYTEKASLSIRTLEEEQQKIPRHHRFTNERNKIGFSPLAK
                     QLPKWIEEAKGYLKEHKEKRV"
     gene            complement(4445..>4584)
                     /locus_tag="VCA20_002197"
     CDS             complement(4445..>4584)
                     /locus_tag="VCA20_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEP1835929.1"
                     /translation="YISSGAAIHAAIEVAKELGTGKKVLAILPDNGERYLSTALYEFN
                     D"
BASE COUNT         1424 a          825 c         1012 g         1323 t
ORIGIN      
        1 gttagttaac ttaatgacat tggggcagat gccgaacctt tttctcttga ttagagagcg
       61 tagcttgtga gcgatccctt atgccaaaat tgtttccttc tgtccatcaa gagtgatggc
      121 ctaggagtga tttgcatagt ttatagtcat gtttagttta ttttgccgta cccaagtata
      181 gcctgcatca cgttgctttg aactaatatt tcctactttg gatggagttc ttttacttat
      241 tttttgttac aatagtatag aaaggatgga gaagatgcgg aaagttcaat tttttctact
      301 aatagaaagt atgattttta cgatggcagc ctttgacgtg gtagccaatg aggtcagtcg
      361 gaccctgcta cttttctctg ccttattact cgtcatttgg tacttcctag gaagaaaagt
      421 caacagtgtt ttactggtca gctccttatc tctattattt ttggtctttg ttttaaatcc
      481 cttcttcatt attgggatta tgctactagt cgtgtacatg ctgattaatt tcttttcacg
      541 ttatgaaaag agaaaccaat atacccacat tgtttttagt gactattctc tacaggttca
      601 aaaagagaaa aatagatggt ttggcaacca cgaccactct caagataggt ttggttttga
      661 agatattaac attgtacgct tgtttggcaa tgatgtagtt gacttggata agactgtatt
      721 agttggtaga gataatattg ttgttattcg taaaacgttt ggacggacaa aaattattgt
      781 tccaattgat gttgaggttg cactttcagc aactagtatc tatggacgag taagtttcct
      841 gggtgaatca tactgggatt tgcggaatga aagtatagcg gtcaatagtc ccgattatca
      901 agaatcacac aaacgtgtaa aagtcgttac caacaatata ctgggagatg tggaggtggt
      961 tcgcgtatga gaaaacgtac ctacctactc ctcgcctggt ttgcctgtct cattgttttt
     1021 gtggtgattg cttccacact cccgctttta aattattcat tgctagatgt tacgctctgg
     1081 atttcaacgg agcagctcgt gtttactctc attctactag ttatcgtttt aacccttttt
     1141 ctagctgtaa tggtacaaac tgtcagccta gcctccactc aggttatgcg gacgaagtta
     1201 caagctatct tgaagaataa aagtattcga acagataccg aaagtgatca gctcttgctc
     1261 cagttgtctg ataaggtcag aaacctcacg cgtcaggtgc agctggttga caatcaagat
     1321 ttagtcaaga aagaagagat tgttgaggga gaacggaaac ggattgccag ggatttgcat
     1381 gatacggtta gtcaagagtt atttgcaacg agcatgattt tgtcaggatt atcgtccaat
     1441 ctatccactc ttccgcagga aactttgcag gaacagctga tgctcgtgaa agacatgatt
     1501 gagtctgccc aaagagacct acgcatccta ttattacatc tacgcccaag tgaacttgaa
     1561 agtaaaacct tggttgaagg atttgagctg attttgcgtg aagttagcga caaaagtaat
     1621 attatcgttc atttccaaca tgaagtagag gagctaccga aactaattga agaacatctc
     1681 ttccgcattg cgcaagaaat tatcagtaat accttgcgac atgccaaagc caagcatttg
     1741 gatgtttacc tcttgcagaa ggaaacagaa cttcagttga aaatgacaga cgatggtatc
     1801 ggcttccaac aaggtgcaga tgatgaatta agttacggtc ttcaaaacat acgtgagcga
     1861 gttgaagaca tggctggaac gataaaaatt cgcacagctc caaataaggg agtggcgatc
     1921 gatattcgta tcccactatt gaaaggaaaa gaagatgaat acgattcgag tgatgctggt
     1981 tgacgaccat gagatggttc gtctaggatt gaaaagttac ttaaatttac agccagatgt
     2041 tgaagtagtt gcagaagcta gtgatggcga ggaaggtttg gctaaagcat tagaggttaa
     2101 acccgatgtt gtagtgatgg acttggttat gcccaagatg actggcgtag aagcaaccct
     2161 ggccttgctg aaggaatggc cgcaagcgca gattgtcatc ctgacctcct acctagataa
     2221 tgaaaaaatc tatcctgtgc tggaagcagg agctcgtggc tatatgttga aaacgtcaag
     2281 tgctgacgaa atcttagtag ccatccgcaa ggtagctctt ggagaatatg ctattgagac
     2341 agaagtagag aaaaaagtag agcaccataa acgccaccca gatttacatg atgacttgac
     2401 agctcgtgaa cgcgaaatct tgacgctgtt agccaaggga tatgataatc aacgaattgc
     2461 agatgagtcc ttcatttccc taaaaaccgt taagacccat gtttccaata tcttatccaa
     2521 actagctgtt agcgatcgta ctcaagcagt tatttatgct ttccagcatg gtttggtggc
     2581 gcaagaggac caatagtggt ataatgaaaa ttagttaatt ttcaaccaaa ggatagtgga
     2641 attatatgga tgcaaaactc agatataagg caaaaaaact aaaaatagtc ttttttgaca
     2701 ttgacgacac gctacgtgtg aagaatacag gctacattcc tgaatcaatt cagcaggttt
     2761 tcaaatcact gaaagaaaag ggaattttga caggaattgc gtcaggacga acgccttatg
     2821 gtttggtgcc agaaatcaag gccttaaagc cagacttttt cgctatgatt aacggctcct
     2881 acgtagaaga tgccaaaggt caggtagtgt accatcagcc gatgccccaa aatttagtag
     2941 aatctgtatt aaattgggct aaagaaattg gaatcgaata tggtatgctt ggtagtcaaa
     3001 aagggacact ttctgcacga actgaccgca ttagtcaagt aattgacttg atttatgaag
     3061 gtttggaaac aaacccaact ttttataaag aaaatgacat ctatcaattg ttaactttcg
     3121 agaaagatgg tcacgaagta gaacttccag aagagttgca agcggaattg cgtagtgttc
     3181 gttgggatgc tatttcatcg gacattgtgc taaaaggctc ttccaaagca actggtgttg
     3241 ctaaggttgt tgaaaaacta ggcctgaaac ctgaaaatgt cctagtattc ggagatggac
     3301 tcaatgatat tgagttattc gattatgcag gaattagtat cgctatgggg cattcgcatc
     3361 cagaactgca aaagcatgca gattatatca caaaaaaagt agaagaagat ggcatttttg
     3421 atgccttgga gaaattaggt atggtagaaa aagaaaaata tttcccgcaa ttagatttgg
     3481 aaaatgttac aggaccagtt gcgcatatca agactaacca tggtaagtta acagtaaaac
     3541 tttttcctga aattgcaccc aaaactgtag caaatttcgt agctctttct aaagatggct
     3601 attatgacgg gattatcttc catcgtatta tcaaagactt tatgattcaa ggtggcgatc
     3661 caaccggaac aggtatgggt ggtgaatcta tttatggtac tgcgtttgaa gatgaatttt
     3721 caatggaagc ttttaatctc agaggagcct tgtcaatggc taatgcagga ccaaatacaa
     3781 atggaagcca attctttatc gttcaaaacc aaaacttccc atacaatgcg aaagaattag
     3841 aacgaggcgg ttggcctaaa gaagttgcag aagcctatgt caaaaatggc gggacaccac
     3901 atctagacca acgccacaca gtgtttggtc atctagttga tgaagaatcc tttgtcgtct
     3961 tggatgctat cgcagcggtt gcaacagatt cagcagatcg tccacatgaa gatgttgtaa
     4021 ttgagaccat cgaaatagag gattaagatg aaaatcggag ataaactcaa ggggacggta
     4081 acgggcattc agccgtatgg agcctttgta caattagaga atgggacgac gggcctaatc
     4141 catatctcgg aaatcaaaac aggttttgtt gataatattt atgatcattt aaaattgggg
     4201 caagaggttt tggtacaggt agttgatttt gatgaatata ctgaaaaggc tagtttatca
     4261 atccggacct tagaagaaga acaacaaaaa attccgcgcc atcatcgttt tacaaatgaa
     4321 cggaataaaa ttggtttttc ccccttagca aaacaacttc cgaaatggat tgaggaagct
     4381 aaaggctatc tcaaagaaca taaagaaaag agggtatagg atgcattcct aaccttcttt
     4441 ctttttaatc attgaactca taaagagctg ttgacaagta acgttctccg ttatcaggca
     4501 agattgccaa cacttttttt ccagttccca actccttagc gacttcgata gcagcgtgaa
     4561 ttgctgcacc tgaagaaata tagt
//
LOCUS       DATUCG010000159         4574 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.161, whole genome shotgun sequence.
ACCESSION   DATUCG010000159 DATUCG010000000
VERSION     DATUCG010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4574
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.161"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(328..468)
                     /locus_tag="VCA20_002198"
     CDS             complement(328..468)
                     /locus_tag="VCA20_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835930.1"
                     /translation="MIGHAINGIEGAMFAMLYEADRTYYRCQHCGYRFSRKENRFISN
                     IE"
     gene            828..2108
                     /locus_tag="VCA20_002199"
     CDS             828..2108
                     /locus_tag="VCA20_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HEP1835931.1"
                     /translation="MTREFSFETLQLHAGQEVDSASKSRAVPIYQTTSYVFEDAQEAE
                     DLFALRKSGNIYTRITNPTVSTFENRVAALEGGIGALATSSGMAAITYTILALAHAGD
                     HIVAATTLYGGTFNLFKETLPRYGITTSFVDIDDFEAVEAAIKDNTKLVFIETLGNPV
                     INIPDLEKLAEIAHTHHLPLVSDNTFATPYLINVFSHGVDIAVHSATKFIGGHGTTIG
                     GVIVDSGKFDWAASGKFPQFVEEDPSYHNISYTRDIGAAAFIVAVRVQLLRDTGAALS
                     PFNAFLLLQGLETLSLRVERHVSNAEKIVDFLLSHPQVESVNYPSLPDSPYKALADKY
                     LPKGVGSIFSFQAKGGAEEARKVIDSLEIFSNLANVADAKSLVVHPATTTHAQLAPED
                     LLADGVTPNQIRLSVGLENINDLIEDLQLALDKL"
     gene            complement(2186..3718)
                     /locus_tag="VCA20_002200"
     CDS             complement(2186..3718)
                     /locus_tag="VCA20_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEP1835932.1"
                     /translation="MLLATQHLTLDHQKDLRNLVHDLNLIVNPGDKIAIIGEEGNGKS
                     SLLLTLMDSTLVADYLLISGSIHRYFHSPVYIPQGLPLEMAELTLNDYFFGDMDSDID
                     YHLLYLYAEQLQFNSERFASQQLIGSLSGGEKLKIQLIKSLATPSDIIFLDEPSNDLD
                     LDTLLWLENYIITSPKTILFVSHDEDFLSKTATKIIHLESVKKKTLAQTKVEELDYQD
                     YALRRHQAYQKQVQQARNDKKEFDKAMNKHLRQKSQVRHTLLNTHDATLGRLIAKKMK
                     NVLSREKRYERMKENLIQVPFHEDTISLFFSDISSLPARKQVLHLENFQLNIGGRQLV
                     RNIHFNLNGQEKIGIIGQNGIGKSSFLKIIYQKLRQRADINLGYMPQHYTEVLDESNT
                     PLDFLLENGNREQEQLILTHLASLQFTRQEVHHKISELSRGQKAKLLLLKMVLEQANF
                     LLLDEPSRNFSPTSQPYIRQLFADYPGGLVCVSHDRRFLKEVCQRIYRLTENGLEELE
                     QI"
     gene            4019..4510
                     /locus_tag="VCA20_002201"
     CDS             4019..4510
                     /locus_tag="VCA20_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEP1835933.1"
                     /translation="MAITGIEQADILEIDKQVRLRRYDGQHDFAFEWHQDLELVWLID
                     GDQERYSLDLLNRMYDYLSKNGECYFIEIFEDGQFIPIGDVTLFADDFAIAIGDKRYW
                     EKGIGTKVLHRMIERAREVGLEEILVEEIYDWNTGSRKLFEKCGFEAVEKTKKGWSYK
                     MIL"
BASE COUNT         1340 a          782 c          946 g         1506 t
ORIGIN      
        1 aattgcgata cgtttcttga gatactttag tcctgctctt gtttcagttg actataacca
       61 taacgcattg gtttcctcct ggataaataa aactattatc ccaagagaat agacaaaaaa
      121 gaagagccaa tcagctcttc taaatcattc aattttagct atcaatcatt aagtattttg
      181 gttcaaattg gttcaactga tatggagagt tatccatttt agtaaatatt tatacaggtt
      241 tataagcaat tattctgtat attcctacat agttacggat aagattgcat aaaatatcgg
      301 tgtatatttc tattaaaatt agggaggtta ttcgatgtta gaaataaaac gattctcctt
      361 tctagaaaat cggtaaccgc aatgttgaca acgataataa gtccgatcag cttcatagag
      421 cattgcaaac atagctccct ctatgccatt gattgcatga ccaatcatac cggctttacg
      481 agaatatctt ttttgtgttg gaacgtaagt ttgcagtttt attgacttac attttggaca
      541 tttgagcgct tttttagtgt ccatattatt aggatggcat ctgcgataga tgctataaga
      601 gattattagt actatgagga taatcggacc gatatattct attaaggtat tcatattctt
      661 attgtaaatc ttgtccttat tttattcaag gaaaatgaat attatttttt ctaaaataac
      721 tgatcctatg gctatagttg ttaactatac atatctctaa tgtttaagta ttagtcaaga
      781 tggtaaaaat gaggtagaat acataaaaag accaaaggag tacctttatg actagagaat
      841 tttcatttga aacccttcaa ctccatgctg gacaagaagt tgattctgct tcaaaatcac
      901 gtgctgtacc catttatcaa accacttcct atgtttttga agatgctcaa gaagcagaag
      961 atttatttgc attgcgtaaa tcagggaata tctatactcg tatcactaat ccgacggtct
     1021 ctacttttga aaatcgtgtg gcagctttag aaggtggtat tggtgccttg gccacttctt
     1081 caggaatggc agccatcaca tacactattc tagcattagc gcatgctggt gatcacattg
     1141 tggcggcaac gacactctat ggtgggactt ttaacctatt caaagaaacc cttcctcgct
     1201 atggtattac aacaagcttt gtagatattg atgattttga agcggtagaa gctgcgatta
     1261 aagataatac aaaactggta tttattgaaa ccttgggaaa tcctgttatc aatattcctg
     1321 atcttgaaaa attggcagaa attgcacaca cccaccacct cccgcttgtt tcagataata
     1381 ccttcgcaac tccatatttg attaatgtat tttctcatgg tgttgatatt gccgtccatt
     1441 cagcaaccaa gtttatcggt gggcatggta cgaccattgg cggtgtgatt gtcgatagcg
     1501 gcaagtttga ttgggcggca tctggtaagt ttccgcaatt tgttgaagaa gatccaagct
     1561 atcataacat tagttatact cgtgatattg gtgcagctgc atttattgtt gcagttcgtg
     1621 tacaattact tcgcgataca ggtgctgccc tttctccatt caacgccttt ttgcttttgc
     1681 aagggttaga aaccttgtct ctccgtgtag agcgtcatgt atctaatgct gaaaaaattg
     1741 ttgacttttt acttagccat cctcaggtag agtcggtcaa ttatccatca cttccagata
     1801 gtccttataa ggctttggcg gataagtacc tgcctaaagg tgttggatct attttctctt
     1861 tccaagcaaa aggcggagca gaagaagccc gtaaggttat tgatagtttg gaaatctttt
     1921 ccaatttagc aaacgttgcc gatgctaaat cactagtagt tcatccagct acaactacgc
     1981 atgctcagtt agctccagaa gatttacttg ctgatggggt cacaccaaat caaattcgcc
     2041 tatctgttgg ccttgaaaat atcaatgatt tgattgagga tttgcaactt gctcttgata
     2101 aattataggt tgtgaatcga atagcatgac ttcattcttt atgaataaat aaaaggaaat
     2161 ttggcgtgtg tgccatttta cttttttata tctgttctag ttcttccagt ccattttcag
     2221 ttagtcggta gattctttga caaacctctt tcaaaaagcg tctgtcgtgt gatacgcata
     2281 ccaagccacc tggataatcg gcaaatagtt gacgaatata tggctgagaa gtaggagaga
     2341 agtttcgact gggttcatct aataagagaa aattggcttg ttcaagaacc atttttaaca
     2401 agagtaactt ggctttttgt cccctcgaaa gttcactgat cttgtgatga acttcttgtc
     2461 gcgtaaactg cagactggct agatgcgtta agataagttg ttcctgctca cgattgccat
     2521 tttctaataa aaaatcaagt ggagtgttag actcatcaag aacttctgta tagtgctggg
     2581 gcatgtagcc caagtttata tccgctcgtt gcctgagttt ctgatagatg atctttagaa
     2641 agctggattt accaattcca ttttgtccaa tgatgccaat tttctcttgt ccatttaagt
     2701 tgaaatggat atttcgaact aattgtcgcc ctccaatgtt caattggaag ttttctaagt
     2761 gaagcacttg ttttctagct ggtagtgatg aaatgtctga gaagaagagg gaaatggtat
     2821 cttcatgaaa aggaacttgt ataagatttt ccttcattct ttcatagcgt ttttcacgtg
     2881 agagaacatt tttcattttc ttagcaatga gccgtcctag tgtggcatca tgggtgttga
     2941 gcaaggtatg acgaacctgt gatttttgac gtaggtgctt gttcatagct ttgtcaaatt
     3001 cttttttgtc gttccgagct tgctggacct gcttctgata agcttgatgg cgtctcaatg
     3061 cgtaatcctg gtagtccagt tcttcaacct tggtttgagc aagtgttttc ttcttaacag
     3121 attctaggtg aatgattttt gtagctgttt tactgaggaa gtcttcatcg tgggaaacaa
     3181 atagaatggt tttaggacta gtaatgatat agttttcaag ccaaagtaag gtatctaaat
     3241 ccaagtcatt ggacggctca tccagaaaaa taatatcaga tggagttgct agagatttga
     3301 ttaactgaat ttttaatttt tcgccacctg acaggcttcc aatcaattgt tgactggcaa
     3361 atcgctcgct gttaaactgg agttgctcag catagagata gagtaggtgg taatcaatgt
     3421 cactgtccat atctccgaag aagtagtcgt tcaaggttag ctctgccatt tccaatggaa
     3481 gaccttgggg aatgtagact ggcgagtgaa aatatcggtg aatgctgcct gatatgagta
     3541 ggtagtcagc gacaagagtt gaatccatca aggtgagtaa tagactggat tttccatttc
     3601 cttcttcgcc gatgatagct attttatcgc cagggttgac aatgaggttt aggtcatgaa
     3661 ccagatttct caaatctttt tgatggtcga gggttaggtg ttgagtggct agtagcataa
     3721 ggcctccttt accagaaaaa aacacactac ctatttagta gtgtgtcgaa atctgctcaa
     3781 aaagacccta ttcaaggaat agagggaaca gaaaaatgga cgcaagaaaa ctactaaata
     3841 agtatagaac ttagattgag tttacttgtt attgtccatt tctccttacc tcacatactt
     3901 tatttgcttt agtatagcat atcgataaaa tcttgtcaat cagcatgcta taaaatttat
     3961 tgatattgat agagatgtat gttaaaatga acgtattgta aatgagagag gatacgaaat
     4021 ggcaatcact ggaattgagc aagcagatat tttagaaata gacaaacaag tccgtttacg
     4081 tcggtatgat ggtcagcatg attttgcttt tgaatggcac caagatctag aattggtctg
     4141 gctgattgat ggtgatcaag aacgttatag tcttgacttg ctcaatcgta tgtatgatta
     4201 tttgtctaaa aatggcgaat gttactttat tgaaattttt gaagacggtc agtttatccc
     4261 aattggtgat gtgacgctgt ttgcagatga ttttgccatt gccattggag ataagcggta
     4321 ttgggaaaag ggaatcggga ctaaggtgtt acatcgaatg atcgagcgtg caagggaagt
     4381 gggtcttgag gaaatacttg tagaagaaat ttatgactgg aatacaggct cccgtaaact
     4441 atttgagaaa tgtggctttg aagctgttga aaagacaaag aaaggttggt cctataagat
     4501 gatattgtaa attaatttgg agataccaaa aaaacttgac gaaagtcaag gcttcagaac
     4561 gtagataaac tctt
//
LOCUS       DATUCG010000160         4487 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.162, whole genome shotgun sequence.
ACCESSION   DATUCG010000160 DATUCG010000000
VERSION     DATUCG010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4487
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.162"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(94..2427)
                     /locus_tag="VCA20_002202"
     CDS             complement(94..2427)
                     /locus_tag="VCA20_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009659728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEP1835934.1"
                     /translation="MNNKIIETLEFHKVRQKIEPYLLTEQGFEELRQLEPMMEVHRIQ
                     QAFDELTDIAQIFVENPYFSLAATSDIGPAMRRLELDTDLNIAELLAVKKVLEVSKSL
                     LDFYGNLENVSLSQLDKLFEKIELFPHLQGSLQSINDAGFVEDFASEKLARIRRKIRE
                     AEDQVRQVMQDILKTKGDMLSDSILASRNGRNVLPVKNTYRNKIAGVVHDISASGSTV
                     YIEPRAVVTLNEEISHLRAEELHELNRILQELSDMLRPHSGVIRNNAWLIGHIDFVRA
                     KHLFAREHQAVVPKLSEKQDIALLNVRHPLIAKPVPNDLYFGSRLTAIVITGPNTGGK
                     TIMLKTLGLTHLMAQSGLPILADKGSRVAIFKEIFADIGDEQSIEQSLSTFSSHMTHT
                     VEILRAADQDSLILFDELGAGTDPQEGASLAMAILDDLRLRGIKTMATTHYPELKAYG
                     IETSGIENASMEFDSNSLRPTYKFMQGVPGRSNAFEIARRLGLSDVIIQSAQSWTDTD
                     SDVNRIIEKLESQTVESRQRLDKIRDVEQENYKMNRALRKLYDELNRERENEFNKARL
                     EAKEIVDMALAESEDILKNLHAAASLKPHQIIEAKAELKKLAPEVVDLSKNKVLKKAK
                     IQREAKVGDDIIVTAYGQRGTLTNQLKDGRWEAQVGLIKMTLAKDEFELVKAEKAEQP
                     KKRQVHTVKRANVRGPKARLDLRGKRYEEAMMELDEFIDQALLNNLAQVDIVHGIGTG
                     VIREGVTKYLRRNKQVKEFGYAPQNAGGSGCTIVTFK"
     gene            complement(2770..3318)
                     /locus_tag="VCA20_002203"
     CDS             complement(2770..3318)
                     /locus_tag="VCA20_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEP1835935.1"
                     /translation="MISLAIILILAWSFYIGYSRGLVLQAFYSMGSIIALVVATATYK
                     KLASFLYLWVPFANATQGSSNYYFDEKYLFDLDMVFYAGLAFLLIYVLVYAIVRFIGI
                     FVHLLEAFNPDTKTTNLISGALAVLVTFISLQIVMVLLSTIPLAMIQDKLHSSFLANI
                     MIQYTPFTSSFLKSLWLSNIAG"
     gene            complement(3315..3626)
                     /locus_tag="VCA20_002204"
     CDS             complement(3315..3626)
                     /locus_tag="VCA20_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835936.1"
                     /translation="MANLNRYKFTFGEKQLTLTTEHDNLFMEEIERIAQEKYQAIKEK
                     MPTADPETLALLLAINALSVQLTREMVFEQKEQELASVKEKVLKKNVTLIDLDELEDK
                     V"
     gene            complement(3684..4397)
                     /gene="treR"
                     /locus_tag="VCA20_002205"
     CDS             complement(3684..4397)
                     /gene="treR"
                     /locus_tag="VCA20_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEP1835937.1"
                     /translation="MKKYQEIYNDLKEKIRTNVYPAESSLPTEQQLQEIYGVSRDTVR
                     KALAILTEGGLIQKVQGRGSMVLKQEILNFPVSGLTSYQELTNVLQLSTKTDVVSLDM
                     ITVNSSLSHLTGFEPYSKVWEVVRTRSIDGKVSVVDTDYLAVDVVPELTTAIAEKSIY
                     EYLENKLGLDIAYAQKEITVEPTNREERELMQSKDDYLVLIKSRVYLGDTQQFQYTES
                     KHKIDEFRFVDFARRKHSL"
BASE COUNT         1276 a         1034 c          868 g         1309 t
ORIGIN      
        1 gttgttttga gtttatgata cgcaaagtgt ttttatttag ctagaattaa ctagcacaac
       61 gggtaatcat aagaatctcc catcggttga ttgctattta aacgtcacaa tagtacagcc
      121 tgagccacct gcattttgtg gggcatagcc gaactccttg acttgcttgt ttcggcgtag
      181 atacttggtc accccttctc ggataactcc agtaccaata ccgtggacaa tatctacttg
      241 tgctaggttg ttgagcaggg cctggtcgat aaattcatcc agctccatca tagcctcttc
      301 gtagcgtttg ccacggagat ctaagcgggc ttttggtcct cgtacattgg cacgtttgac
      361 tgtgtgaacc tggcgtttct taggctgctc tgccttttcc gccttgacta actcaaactc
      421 atctttggcc aaggtcatct taatcaagcc aacctgagct tcccagcgcc cgtccttgag
      481 ctgattggtc aaggtcccac gttgtccata agctgtaacg ataatgtcat cgcccacctt
      541 ggcttccctc tgaattttag ctttcttcag aaccttgttt ttagacaaat ccaccacttc
      601 aggcgccaac tttttcagct ctgccttggc ttcaataatc tggtggggct taagactagc
      661 tgcagcatgg agatttttga gaatatcctc gctttctgcc aatgccatat ctacaatttc
      721 cttggcttcc aagcgtgcct tgttgaactc attttcccgc tcacgattga gctcgtcata
      781 gagcttgcgg agggctcggt tcatcttgta attttcttgc tccacatcac gaatcttatc
      841 cagacgctga cgactttcaa ccgtctgact ttccaatttc tcgataatgc ggttcacatc
      901 gctatctgta tccgtccaag attgggctga ctggataata acatcagata gaccaagacg
      961 gcgggcaatt tcaaaggcat tggagcgacc aggaacaccc tgcataaact tatatgtcgg
     1021 acgcaggcta ttgctatcaa attccatgct ggcattttca ataccagagg tttctatccc
     1081 gtaggcctta agctcagggt agtgggtggt cgccatggtc ttgatccccc gcagacgaag
     1141 gtcatccaaa atagccattg ccaaagacgc accctcctgc ggatctgtcc cagctcccaa
     1201 ctcgtcaaat agaatgagag aatcttgatc agctgctctt aaaatttcca ccgtgtgggt
     1261 catgtgactg gagaaggttg ataaactctg ctcaatcgac tgttcgtccc caatatccgc
     1321 aaaaatttct ttgaagatag ctacccgact gcccttatca gccaagatag gcaaaccaga
     1381 ctgagccatc agatgggtca atcccaaggt cttgagcatg atagtcttac cacctgtgtt
     1441 gggacctgtt atcacgatag ccgtcaaccg actaccgaaa taaaggtcat ttggtacagg
     1501 cttagcaatg agcgggtgtc gaacgttgag aagggcaata tcctgcttct cagatagttt
     1561 gggtacaact gcttgatgtt cacgcgcaaa gagatgctta gcacggacga aatcaatatg
     1621 cccgataagc caggcattgt tacggatcac accactatgc ggacggagca tatccgacaa
     1681 ttcctgcaag atacggttga gctcatggag ttcttctgca cgtaggtgac tgatttcttc
     1741 gttcaaggtc accactgccc gcggctcaat ataaacggtc gaacccgaag cagaaatgtc
     1801 atggacaacc cctgcaattt tattgcgata ggtattttta acaggaagga cattgcgtcc
     1861 attacggctt gccaaaatgc tatcggacag catgtccccc ttagtcttca aaatatcctg
     1921 cataacctga cgaacttgat cttcagcttc acggattttt cttcgaatac gggctagctt
     1981 ttctgaggca aaatcctcta caaaaccagc atcattgatg gactggaggg agccctgtaa
     2041 atgtggaaaa agctcaatct tctcaaagag cttatccagc tgactaagac tgacattttc
     2101 cagatttcca taaaaatcca agagagattt agaaacttcc aagacctttt taacagctaa
     2161 taattcagcg atattgaggt ctgtatccaa ttccaaacga cgcatggctg gaccgatgtc
     2221 actagtagca gccagactga aatagggatt ttccacaaaa atctgcgcta tgtctgtcaa
     2281 ctcgtcgaag gcctgttgga tacgatggac ttccatcatg ggctccaact gtcgtaattc
     2341 ttcaaagccc tgttccgtca agagataggg ctcaattttt tgtcttacct tgtgaaattc
     2401 aagggtttca ataattttat tattcatata ttcctactta gtcttttcct tagctttatc
     2461 tcattatcaa aacaataaag ataaaaatat agtctttccc aatctcttac attaaaggta
     2521 tagcaatcaa ctaatactca atgaaaatca aaatcagact tgctccaagg gttcggggga
     2581 acctttagag gttggaaata ggcgaacgta gttcgtttcg ctctgatgca gatagaactc
     2641 tgttcctttt atatttcaag ggagcaggct gaaaagatcc actggatctt ttcactcatc
     2701 aaatcaacaa cgtctggttt tgattttcga agagtattat aaatgacgat aaaaaggata
     2761 aggtctccct taccctgcta tattactcaa ccagagagat tttaaaaagc tgctagtgaa
     2821 tggtgtgtac tgaatcatga tgttggcaag aaaactactg tgcaatttat cttgaatcat
     2881 cgctaacgga atagttgata gcaagaccat cacaatctga agagaaataa aagtcaccaa
     2941 gacagccaag gcgccgctta tgagattggt cgtttttgta tcaggattaa aagcctctaa
     3001 tagatggaca aatattccta tgaaacgcac aatagcataa accaagacgt atatcaaaag
     3061 aaaggccaat cccgcataaa ataccatatc taggtcaaag agatatttct catcaaaata
     3121 atagttggag ctcccctgag tagcattggc aaatggcacc cagagataaa ggaaggatgc
     3181 caatttttta taagtagccg ttgcaaccac cagagcaata atacttccca tgctataaaa
     3241 ggcctgtaga actagaccac gactgtagcc aatgtagaaa ctccatgcca aaatgagaat
     3301 aattgctaat gaaatcatac cttgtcctca agctcatcta agtcaatcaa ggtcacattc
     3361 tttttcaaga ccttttcttt gacgcttgca agttcttgtt ccttttgttc aaataccatc
     3421 tcgcgcgtta gttgaacgga tagagcattg attgccaaca aaagcgcgag ggtttcaggg
     3481 tccgccgtcg gcatcttttc tttgatggct tggtattttt cctgtgcaat acgctcaatc
     3541 tcttccatga ataaattatc atgctcagtt gtcaaggtta attgcttctc gccaaatgta
     3601 aatttatatc gatttaagtt tgccatatct tacctcttct tctatctatt atagcgtaaa
     3661 aaacttgatt tggctaatct ctcctataaa gaatgctttc tacgagcaaa atctacaaag
     3721 cggaattcat caattttatg cttgctttct gtatattgga attgctgggt atcaccgaga
     3781 tagacacgag atttaatcaa gaccaaataa tcatccttcg attgcattag ctcacgctct
     3841 tctcgattgg tcggctctac cgtaatctct ttttgtgcat aagcaatatc aaggcctaac
     3901 ttattttcta ggtattcgta gatggatttt tcagcaatag ctgttgtcaa ctctggcacg
     3961 acatcgacag caagataatc tgtatccaca acggagacct taccgtcaat cgaacgtgta
     4021 cggacaactt cccacacctt gctatacggc tcaaagcctg tcaaatgcga aaggctacta
     4081 ttaacggtaa tcatatctaa gctgacaaca tctgtcttgg tagaaagctg gagaacattt
     4141 gtcaattcct gataggaagt taaacctgaa actgggaaat tgagaatttc ttgcttaagg
     4201 accattgaac cacgcccttg cactttttga atcaaacctc cctcagtcaa aatcgccaac
     4261 gccttacgaa ccgtatcacg actaacacca tagatttcct gaagctgctg ttctgtcggt
     4321 agggagcttt ctgccggata aacatttgtc cgtatttttt cttttaagtc attataaatt
     4381 tcttggtatt ttttcatcat ttcctcgcaa attcatctgt caaataacat acaactatca
     4441 acattataac aaaattcatt tcaaaattaa aaaaactaag caaaaac
//
LOCUS       DATUCG010000161         4473 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.163, whole genome shotgun sequence.
ACCESSION   DATUCG010000161 DATUCG010000000
VERSION     DATUCG010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4473
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.163"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(212..2908)
                     /gene="ppc"
                     /locus_tag="VCA20_002206"
     CDS             complement(212..2908)
                     /gene="ppc"
                     /locus_tag="VCA20_002206"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEP1835938.1"
                     /translation="MAIQKLENYNNKEAIREEVTILTSILEEVAAQMIPAETFAKIVE
                     LSQLSRQDDYQALIAIISQLNNDELAVISRYFAVLPLLINISEDVDLAYEINHQNNIV
                     QDYLGKISTTIDMVSKQENAAEILEKLNVVPVLTAHPTQVQRQSMLDLTKHIHELLRR
                     YRDVKLGLLNKNKWEDELRCYIEIIMQTDMIREKKLKVTNEITNVMEYYNSSFIKAVT
                     KLQREYKRLAAEKGIVLNNPRPITMGMWIGGDRDGNPFVTAETLKLSALTQCEVIMNY
                     YDDQLYKLYRNFSLSTSIVNVSTAVKMLADLSEDSSGYRENEPYRRAFHYIQMKLANT
                     RDYLVHNKPSDVRYSNVAEFKADLLAIKQSLIENKSMALLKGDFTELLEAVEAFGFYL
                     ASIDMRQDSSIHEASVAELLASARIVEDYSSLSEEAKCHVLLKQLETDPRILSATHVP
                     KSEQLEKELAIFAAARELKDKLGEEIIKQHIISHSESVSDLLELAVLLKEVGLVDVDK
                     ARVQIVPLFETIEDLDNAPDTMRQYLQLDLAKRWIAGNKYYQEIMLGYSDSNKDGGYL
                     SSGWTLYKAQNELTQIGQAYGVNITFFHGRGGTVGRGGGPSYDAITSQPFGSIKDRLR
                     LTEQGEVIGNKYGNKDAAYYNLEMLVSATLDRMVTQMITDPNEIDGYRETMDEIVSDS
                     YRIYRDLVFNNPHFYDYFFAASPIREVSSLNIGSRPAARKTITEIGGLRAIPWVFSWS
                     QNRVMLPGWYGVGSSFKRFIDKHPDNLSKLQKMYESWPFFRSLLSNVDMVLSKSNMNI
                     AFEYAKMCESEEVRNIFHVILDEWQLTKEIILSIEQNDELLAELPFLKASLDYRMPYF
                     NVLNYIQIELIHRLRRGELSKEQENLIHITINGVATGLRNSG"
     gene            complement(2929..4143)
                     /locus_tag="VCA20_002207"
     CDS             complement(2929..4143)
                     /locus_tag="VCA20_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEP1835939.1"
                     /translation="MKIDKHQLLNYSILIPYLILSVIGLVMVYSTTSATQIINGGNPF
                     RTVINQAGFWIVSLVAIYTIYRMKLSFLRKKAVIYSVILVEVFLLAISRLFTPINGAH
                     GWIPLPIAGTLQPAEYLKLIIIWYLAHEFAKQQADIRTYDYVSLIKGSWIPKEFTDWR
                     VVSLLLLGLVATLPDLGNATIIVLIMVVMISVSGIAYRWFSTAVLGIVAASTVILGTI
                     RILGVETVEKIPLFGYIARRFAAYFDPFGNATNSGLQLTHSYYAMSNGGWLGRGLGNS
                     IEKKGYLPEAHTDFAFSIVIEELGFVGASLILALLFFLIIRIIIVGVRARSPFNAMMA
                     LGMAGMLLIQTFVNIGGISGIIPATGVTFPFLSQGGSSLLIISIGIGFVLNIDASEKR
                     RLIEEEIERTLI"
     gene            complement(4280..4390)
                     /locus_tag="VCA20_002208"
                     /pseudo
     CDS             complement(4280..4390)
                     /locus_tag="VCA20_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646570.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
BASE COUNT         1381 a          979 c          787 g         1326 t
ORIGIN      
        1 atttttgtcc tgctcttgtt tcagttgact atagtaggaa atctcctcgg caatgccgat
       61 gacttctttt tcatattcat ccaatttgat gctcatctcc tcctttcact aatataacga
      121 atagaattct atttggttac acatttttta aaaatattat atcctttacc gtattaaaaa
      181 agcaacctcc gaagaagttg cttaaataag attaaccaga atttcgtaaa cctgtcgcca
      241 caccattgat ggtaatatga atgagattct cttgttcttt gctcaattct ccacgacgaa
      301 gacgatgaat taattcaatc tgaatatagt tgagcacatt gaaataaggc atacgataat
      361 caagactagc ttttagaaac ggtagttctg ctaaaagctc atcattttgc tcgattgata
      421 aaatgatttc tttggtcaac tgccattcat ctaaaatgac atggaagata tttcgtacct
      481 cttcgctttc acacatttta gcatattcaa atgcgatatt catattggat ttagacaata
      541 ccatatccac gtttgataga agtgaacgga agaatggcca agattcgtac attttttgta
      601 acttagacaa attatcaggg tgcttatcaa taaagcgttt gaaacttgaa ccaactccat
      661 accaccctgg caacataaca cggttttgtg accaagagaa tacccatgga atcgcacgca
      721 aaccaccgat ttctgtgatg gtttttcgag ccgcgggacg tgagccaatg ttcagacttg
      781 atacttcacg aattggacta gcggcaaaga aataatcata aaaatgtgga ttgttgaaca
      841 caagatctct ataaatacga tagctatctg atacaatttc atccatggtt tcgcgatagc
      901 catcgatttc atttggatcg gtaatcattt gtgtgaccat acggtcaagc gttgcagata
      961 ccagcatttc taaattatag taggctgcgt ctttgttacc gtatttattg cctatcactt
     1021 cgccttgttc tgtcaaacga agccggtcct tgatggaacc aaatggctga gatgtaatcg
     1081 catcgtagga aggacctcca ccacgaccaa cagtacctcc gcgaccatgg aagaaggtga
     1141 tgtttactcc gtaggcttga ccaatttgcg tcaattcatt ctgtgctttg tagagtgtcc
     1201 agccggagga taggtagcca ccatccttgt tggaatctga gtagccaagc atgatttctt
     1261 ggtagtattt attgcctgca atccaacgtt tcgctaaatc taattgcaga tattgacgca
     1321 tggtatcagg tgcattatct aagtcctcga ttgtctcaaa aagtggaaca atctgaacac
     1381 gggccttatc gacatctact agaccaactt cttttaatag gacagctaat tccagtaaat
     1441 cagaaacact ctccgagtgt gaaataatat gttgcttgat gatttcctcg cctagcttat
     1501 ctttcaattc acgcgcagca gcaaagatag ccaattcttt ttctaattgt tctgactttg
     1561 gtacgtgcgt tgcagaaaga atgcgtggat ctgtttccaa ctgtttcaaa agaacatggc
     1621 atttagcttc ctcagataaa ctggagtagt cttctacgat acgggcagaa gccaatagct
     1681 ccgcaacact tgcttcatga atagaagagt cttggcgcat atcgatacta gctaggtaga
     1741 aaccaaacgc ttcaaccgct tcaagcaatt ctgtaaaatc acctttcaat agcgccatag
     1801 atttattttc aatcaaagat tgtttgatgg ctaataaatc agctttaaac tcggctacat
     1861 tactatagcg aacatctgac ggcttattgt gaactagata atctcttgta tttgcaagtt
     1921 tcatctgaat atagtgaaaa gcacgacgat aaggctcatt ttcacggtag ccagaactat
     1981 cttcagataa atccgccagc atcttaaccg ctgtagatac atttacaatg gaagttgaga
     2041 gcgaaaaatt ccgatagagt ttatagagtt gatcatcgta ataattcata atgacttcac
     2101 actgagtcaa ggctgacagc ttcaaggtct ctgcagtaac aaatggattt ccatcacggt
     2161 ctcccccaat ccacataccc attgtaattg gacgaggatt atttaagacg attcctttct
     2221 cagcggccaa acgtttgtat tcccgctgca acttagtgac agccttaata aaggaagaat
     2281 tgtaatactc cataacgttt gtgatttcgt tggttacctt caacttcttc tcacgaatca
     2341 tatccgtctg catgataatt tcaatgtaac aacggagctc atcttcccat ttgtttttat
     2401 tgagcaagcc caattttaca tcgcgataac gacgaagcaa ctcatgaata tgcttggtta
     2461 aatcaagcat agactggcgt tgaacctgag taggatgggc tgtcaagaca ggaacaacat
     2521 tcaacttttc aaggatttca gctgcatttt cttgtttgga aaccatatca attgttgttg
     2581 aaatcttacc aagataatct tgaacaatat tgttttgatg attgatttca taggccaagt
     2641 caacatcttc tgatatatta atcaaaagtg gcaaaacagc aaagtatcta gaaataactg
     2701 ctagttcatc attattcaac tgactgataa tagcaatcaa tgcctgataa tcatcctgac
     2761 gagacaattg agacaattct acaattttag caaatgtctc tgcaggtatc atttgggctg
     2821 ctacttcttc caaaatagat gttagaatcg ttacttcttc acggattgct tccttgttgt
     2881 tatagttttc taatttttga atagccatct cctacctctt tcctatctct aaattagcgt
     2941 acgctctatt tcttcctcaa ttaatcgacg tttttcacta gcatcaatat tcaaaacaaa
     3001 ccctatcccg atggaaataa tcaaaagact cgatcccccc tgtgacaaga aggggaaagt
     3061 tacccccgtt gcaggaataa ttcctgaaat tcctccgata ttgacaaatg tttgaatcag
     3121 aagcatacct gccattccaa gtgccatcat cgcattaaat ggactacgag cacggacgcc
     3181 tacaataata attcgaataa tcaaaaagaa caataaggct agaattaaac tagctccgac
     3241 aaaaccgagc tcttcaatga caatcgaaaa cgcaaagtcg gtgtgggcct cgggtagata
     3301 cccctttttc tcaatcgaat ttcccaagcc acgtccaagc catccaccgt tactcatcgc
     3361 atagtaagaa tgagtcaatt gaagacctga attggtggca ttaccaaatg gatcaaaata
     3421 ggccgcgaaa cggcgagcaa tataaccaaa gagcggaatt ttttccaccg tttctacacc
     3481 caaaatacga atagtcccta gaataactgt tgaggcggcc acaattccca aaaccgctgt
     3541 tgaaaaccag cgataggcaa tcccactcac actaatcatc acgaccataa tcagcacaat
     3601 aatcgtcgca ttccccaagt ctggcagtgt tgcaactaga ccaaggagga gcagactgac
     3661 cacccgccaa tctgtaaact ctttcggaat ccaacttcct ttaatcaacg atacatagtc
     3721 ataagtccga atatccgcct gttgttttgc aaactcatgt gccagatacc aaatgataat
     3781 taatttcaaa tattctgccg gctgcaaggt tcctgcgatt ggcagaggaa tccagccatg
     3841 agccccgtta atcggagtaa acaaccgaga aatagctaac agaaatactt ctaccaaaat
     3901 aaccgaatag ataacggctt tcttgcgtaa aaaacttaat ttcatacgat aaatcgtata
     3961 gattgctacc aagctgacaa tccagaatcc tgcctggtta atcactgttc taaatggatt
     4021 tccaccatta ataatttgcg tagcactggt tgtcgaatag accatgacca acccaatcac
     4081 cgataagatg agatagggaa tcagtatgga ataattcaac aactggtgtt tgtcaatctt
     4141 cattgcttat ccaaccttct tcgagtatag ctcttattat agcacacttt gtataaaaat
     4201 acttatcact ttctaagttg ctctgcaaac attcggaact aacttttgtc gacaagaaaa
     4261 aggccgaagc ctttcatatc tattcaaatg tttcaagata aaattcaatt tcatccccat
     4321 tcgcaactgt aaggtctttc gcccctttat cagccatttg gccattaact ttatacatcc
     4381 agtacccgtg gtgctagttc atttcaaaat agcgtaagtt atgagctaga ataatttgtt
     4441 ccattcgtaa ctgtagtccg gctaggcttc tag
//
LOCUS       DATUCG010000162         4443 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.164, whole genome shotgun sequence.
ACCESSION   DATUCG010000162 DATUCG010000000
VERSION     DATUCG010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4443
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.164"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            183..1082
                     /locus_tag="VCA20_002209"
     CDS             183..1082
                     /locus_tag="VCA20_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000715517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEP1835940.1"
                     /translation="MRQKKTGQVLQDLISRSDYLVVQGNDLAKAFGNLKAFEHKLLDY
                     CFSYVTKDSVPNERFRVDSHSILKHFSLNASGQNYRRVAEGFKSLNENTALYFSNVRP
                     DGKKSIIMTQLFSFIEFAEDGVVEFKFSEIAQPYVFDLKKNFYSFHLRELARIKGKYA
                     LILMKLWEAHRFGDSRVTLINGTLDEWQEWFLGEEKRLTAGKFMQNVIKRAAEELEDK
                     FNIEIVLDTKKNKRNVIGYEMEIIDKRQPNMAYFEQQQKELDKQREEEMRMNRIFDTN
                     EDGGIFGVSETQMDIYDILKDLE"
     gene            complement(1177..1476)
                     /locus_tag="VCA20_002210"
     CDS             complement(1177..1476)
                     /locus_tag="VCA20_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835941.1"
                     /translation="MTEEEEYKQYIYNNLIIQICESLNICSREFFIIKYNLSLDEINH
                     INDVFKEIVTKNILDYTIFKNKIQEGIPRFNSDNVFQTLLESYKFHQPISQKIKK"
     gene            complement(1476..1991)
                     /locus_tag="VCA20_002211"
     CDS             complement(1476..1991)
                     /locus_tag="VCA20_002211"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835942.1"
                     /translation="MKKFLRKVFVSFLLLITLGGISTPSVSALTEQNNYDIHFKQEEI
                     DIINKGLTPENIQKIENMLEHVTPPTYRIEPRSPQTQAVKKAIKAVIKNRNKIVNLVG
                     KYIGRDAAVSVGASLNTIMPVLRNLQYWSDLAYGTVEGQIKNALVDSGMAETTASTIA
                     YWIRLALEWVI"
     gene            2383..3885
                     /locus_tag="VCA20_002212"
     CDS             2383..3885
                     /locus_tag="VCA20_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HEP1835943.1"
                     /translation="MSYLVARMQKMKVGNLGGAYRHNERIFENHSNKDIDTSRSHLNY
                     ELTDRDRSLSYEKQIKDYVNETKVSSRAIRKDAVLCDEWIITSDKRFFDGLSEEKTRY
                     FFETAKDYFAENYGKSNIAYASVHLDESTPHMHLGVVPMADGKLSSKAMFGREELKKI
                     QEDFPKYINEHGFELSRGQLGSERKHLSVADYKAKIGEETLNKELLSLGAPRYWHEKE
                     NRPGTEEEVAGYESIASLFSEEEYHIREATLEERFKWLDSHRNDLKSDLSRLENILDK
                     KVTEYDEIDSKASERLSELSELESSIKYREKELKGLESDSERLSARVKELTEEKVKQK
                     GVITKRVEQLAAMSVSDLDRRQVAEELQEELTAANPKLFGGGFHFTVDFVNRLKVFMS
                     DVVEKLEETMSQNEMLRRAFENVKVSKERLEQRLTQSDREKSNLEKENYLLREENKEL
                     KGSEKLLEDIQEVLTESEIKSIGNRLDDLQASRRARNRYQERERNQGPSL"
BASE COUNT         1466 a          647 c          957 g         1373 t
ORIGIN      
        1 tgagagttat aaatacaaaa aagtacgaga taatgaccaa aaaagtacga gataatgacc
       61 aaaaaagtac gagataatga ccaaaaaagt acgagataat actcaagttt gcaaaaaaac
      121 gacatttatt gtatagttgc atttagataa ataaatacaa aaaaagtacg tgggatttta
      181 agatgagaca aaagaaaaca ggacaggttt tacaagattt aatatcaaga agtgattatc
      241 tggtcgtaca agggaatgac cttgcgaaag cttttggtaa tttaaaagct tttgagcata
      301 agttattaga ttattgtttt tcttatgtta ctaaagacag tgtgcctaat gaacgattca
      361 gagtagatag tcattcgatt ttgaaacatt ttagtttgaa tgctagtggt caaaattata
      421 ggagagtggc agaaggattt aagtcactga atgagaatac agcgttgtat ttctctaatg
      481 ttcgtccaga tggcaagaaa tcgattataa tgacccaatt atttagcttt attgaatttg
      541 ctgaagatgg agtagttgag tttaaatttt ctgaaatagc tcaaccttac gtttttgacc
      601 ttaaaaagaa tttctattcg tttcatttac gagagcttgc tagaataaaa gggaagtatg
      661 ctttgatact gatgaaacta tgggaagcac atcgatttgg agatagtcga gtaactttaa
      721 tcaatggcac attagacgaa tggcaagagt ggtttttagg agaggaaaag cgactaactg
      781 ctggaaaatt catgcagaac gtgattaaaa gagccgcaga agagttagaa gataaattca
      841 atattgaaat cgtgctggac actaagaaaa acaagcgtaa tgttatcggt tatgaaatgg
      901 aaatcataga taaacggcaa ccgaacatgg cttattttga acagcaacaa aaggaacttg
      961 ataagcaacg tgaagaagaa atgagaatga accgtatttt tgacactaat gaagacggtg
     1021 gaatatttgg agttagtgag acacaaatgg acatctatga cattttaaaa gatttggaat
     1081 aaagccaatt atgacaaaat gcacgtgata tgctagcttt ttgctgtaat agcaaattgt
     1141 tctgattgtt attaaaaaat gagttgaata ttcaactcat tttttaattt tttgtgaaat
     1201 aggttggtgg aatttatatg attctaataa tgtttggaat acgttatcgg aattaaatct
     1261 tggaatacct tcttggattt tgtttttaaa tatggtgtag tctaagatat tttttgtaac
     1321 tatttctttg aaaacatcat tgatgtgatt gatttcgtct agagataaat tatatttgat
     1381 gatgaaaaat tctctggaac aaatatttaa actttcacat atttgaatta ttaaattatt
     1441 gtaaatatac tgtttatatt cctcttcttc tgtcattaaa tcacccactc taaagcaaga
     1501 cgaatccaat aagctatggt tgaagctgtt gtttctgcca ttccggaatc taccaaagcg
     1561 ttttttattt gtccttcaac tgttccgtag gctaaatcgc tccagtattg taaatttctc
     1621 aagaccggca taatggtgtt gagagatgct ccgacagata cagctgcatc tcgaccgata
     1681 tatttaccga ctaaattgac gattttgttt ctatttttta ttactgcttt gatagctttt
     1741 tttacagctt gcgtttgagg tgaacgtggt tctattctat atgttggagg tgttacatgt
     1801 tccaacatat tttcgatttt ttggatattt tcaggagtta atcctttgtt tatgatatct
     1861 atttcttcct gtttgaaatg aatatcataa ttattttgtt cagttagagc tgagacagaa
     1921 ggagttgaaa ttccacctaa tgttattaat aataaaaagc taacgaaaac ttttcttaga
     1981 aacttcttca tgattattct tcctttctat ttttcatttt gctatctatg ttttttatct
     2041 aggatttggc agcatctcct ttctaaccta aataaacttc aaatgaaatg tttccgattt
     2101 cattttatca ttttgtgctt agaaaatcaa aaaagttcct tgttattcga gggctttttt
     2161 gttatctcgc cacctttctt ttgtggcgag caagctgtct gcaagaccct tcggagaacg
     2221 cacatttacg aagtaaagta taatgtgtta tactttactt ggaagttgta ccgaaaatgt
     2281 gctaaactac tagctgatat gttaggtatt taggcaagca atagcctggg cggcttgcct
     2341 gtcattcgtt gttcgaatga ccacctggag aggagctgaa ggatgagtta tcttgtagca
     2401 agaatgcaaa aaatgaaggt tggtaatctt gggggagcct atcgacataa tgaacgtatt
     2461 tttgagaatc attcgaataa agacattgat acgagcaggt cgcatctcaa ctatgagttg
     2521 acggatcgtg accgctcact gtcgtatgaa aaacagatta aggattatgt gaacgaaaca
     2581 aaggtttcta gtcgagcgat tcggaaagat gctgttttat gtgatgaatg gattattacg
     2641 tcagacaaga gattttttga tggtctaagt gaagaaaaaa ccaggtattt ttttgagact
     2701 gctaaagatt attttgcaga aaattatggt aaatctaata ttgcttatgc tagtgttcat
     2761 ttggacgaga gtactccgca tatgcacctg ggagttgttc ctatggcaga tgggaaatta
     2821 tcttcaaagg cgatgtttgg tcgtgaagaa ctgaagaaaa tccaagaaga ttttccgaaa
     2881 tacataaatg aacatgggtt tgagttatcg cgtgggcagt tagggagtga acgaaaacat
     2941 ttgagtgtag ctgattataa ggctaaaatt ggcgaggaaa ctctgaataa agaattgtta
     3001 agcctgggag cacctcggta ttggcatgaa aaagagaata gacctggaac tgaagaggaa
     3061 gttgcagggt atgagagtat agcttcgcta ttctctgaag aggaatatca tattcgagag
     3121 gcaacgttgg aagaacgctt taaatggctg gatagccata gaaacgattt aaagagcgat
     3181 ttaagccgtt tagagaatat cttggataaa aaggttacgg aatatgatga aatcgattct
     3241 aaggcctctg agagactctc agagttatct gaattagaat ctagtataaa atatcgagaa
     3301 aaggaattaa aaggcttaga aagcgattct gagcgtctga gtgctagagt taaagagtta
     3361 accgaagaga aagttaaaca aaaaggggtt attactaaaa gagttgagca gttagcagca
     3421 atgtcggtat ccgatctaga caggagacaa gttgcggaag aactccagga agaattaact
     3481 gcagcgaatc ctaaattgtt tggtggaggt tttcatttca cggttgattt tgtcaatcgt
     3541 ttaaaagtgt ttatgtctga tgtcgttgaa aaattagagg agacaatgag tcaaaatgaa
     3601 atgcttagaa gagctttcga gaatgtcaaa gtgtcaaaag agagattgga acaacggctg
     3661 acacaatcag accgggagaa aagcaaccta gaaaaagaaa actatttgtt gcgagaagaa
     3721 aacaaggaat taaaaggctc tgaaaagctg ttagaagaca ttcaagaggt tttaactgaa
     3781 tctgagataa aaagtatagg gaaccgattg gatgacttgc aggcgtcaag aagagctaga
     3841 aatagatacc aggaaagaga aaggaatcaa ggaccgagtc tctgagcgtt ttattccatg
     3901 gtctatttga caacaagtaa cctaggtcgt tagtctttga tgtgtcgttc ggtcgaactt
     3961 cgttctgacc aaccgcccct ccagtctaac ttgacctagc ttattgtcaa atagaccatg
     4021 gaataaagta agcggtcgtg atataatagc acagtcgcaa cacccagtta gcactgctaa
     4081 ggaggtgaga ctttatgcta aactgcttca tcgagcttgt tctgctacca ctgctggtcg
     4141 gtgtcagcac agaactgatt gcggaatggc taatccgcaa gtggcatgac aaatgcgaca
     4201 aataacttag tctgagtgcc ttgagcaagc acaaaaaaac acctagggtt gcccccctag
     4261 gtgtttcttt atgcttagct tcatcgaact tttaagcaag gtaattatat catgttattt
     4321 tgtttggtgt caaaatttgt gctaaaatat aataaaggct gaggctgagt ttggtttggc
     4381 gacaaatcat tcaaaggttg cgatacgttc gcttcaggta gaggtgctgc ctcctgattt
     4441 tca
//
LOCUS       DATUCG010000163         4428 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.165, whole genome shotgun sequence.
ACCESSION   DATUCG010000163 DATUCG010000000
VERSION     DATUCG010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4428
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.165"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(57..515)
                     /locus_tag="VCA20_002213"
     CDS             complement(57..515)
                     /locus_tag="VCA20_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEP1835944.1"
                     /translation="MRTVEQSLNENKHALHSLVVFRRAANTITKSELETIKKYGLTVC
                     QFGVMEALYNKGNLRIQDLIDKLLSTSGNMTVVIKNMIRDGYIYKTIDTNDRRASLIA
                     LTPLGRETIEKILPEHYDHVGEIFSVLSSEEQDQLAEILKKFKNPHTPNA"
     gene            1007..1504
                     /locus_tag="VCA20_002214"
     CDS             1007..1504
                     /locus_tag="VCA20_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HEP1835945.1"
                     /translation="MEKKIFQLLEWMASKTGQLVLGSFILLSVVTFSIFTIWDIAAAP
                     FNNARSHAVAVATEYADLQTVNDFSIYNGTETYFCVFGVTSQGEEVAVLIPEASSTVY
                     VYPLAQGISQEEAQAIAKKNGASQVERTILGLRDGKPIWEVKSGTAYYLVEFETGNFI
                     KREGL"
     gene            1501..2682
                     /locus_tag="VCA20_002215"
     CDS             1501..2682
                     /locus_tag="VCA20_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002267262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEP1835946.1"
                     /translation="MSKLSRRVLEMEESVTLAAGARAKALKAEGRDILELTLGEPDFV
                     TPKNIQEAAIKSIEDGKASFYTVASGLPELKDAVNTYFENFYGYSIERNQVVLATGAK
                     FVLYAFFVAVINPGDEVLIPTPYWVSYADQIKMNEGVPVFVTATEEHNFKVTVDQLEA
                     ARTDKTKVFLLNSPSNPTGMIYSREELEAIGNWAVEHDILILADDIYGRLVYNGNTFT
                     PISSISESIRQQTIVVNGVAKVYAMTGWRVGFAVGNPEIIAAMSKIVGQTTSNLTTAA
                     QYAAIEAFTGPQDTVETMRQAFEERLNTIYPLLAEVPGFEVIKPKGAFYLFPNVKKAM
                     EMKGYTDVTEFTTAILEEVGVAMVTGAGFGAPENIRLSYATDMDTLKEAVARLHTFMK
                     K"
     gene            2697..4043
                     /gene="asnS"
                     /locus_tag="VCA20_002216"
     CDS             2697..4043
                     /gene="asnS"
                     /locus_tag="VCA20_002216"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEP1835947.1"
                     /translation="MSRKLITINQVKDYVGQEVTIGAWVANKSGKGKLAFLQLRDGTA
                     FFQAVAFKPNFIEKFGEEAGTEKFDVAKRLSQETSVYVTGIVKEDERSKFGYELDVTD
                     LEVIGESQDYPITPKEHGTDFLMDNRHLWLRSRKQVAIQQIRNAIIYATYEFFEKNGF
                     IKFDSPILSGNAAEDSTELFETDYFGQPAYLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSFLSHEESLDLQEAYVKALIQGVIDRAPQALETLERDVD
                     ALKRYIAEPFKRVAYDDAITLLQEHEADEDTDYEHIEHGDDFGSPHETWISNYFGVPT
                     FVVNYPASFKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREDDYDALVAKMDEL
                     GMDKSEYDFYLDLRKYGSVPHGGFGIGIERMVTFVAGTKHIREAIPFPRMLHRLHP"
     gene            4251..>4428
                     /locus_tag="VCA20_002217"
     CDS             4251..>4428
                     /locus_tag="VCA20_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEP1835948.1"
                     /translation="MKQESLGKRIIKLALPATVENIFQTLVGFVDTLLIAQLGLVAVT
                     AVGLANTILNVYLAV"
BASE COUNT         1270 a          837 c         1004 g         1317 t
ORIGIN      
        1 actactttga ttgatgtttg gttatgattt tcgttttctg aaaacgattt ttttcttcat
       61 gcgtttggcg tgtgcggatt cttgaatttt ttaagtatct cggctaattg gtcttgttct
      121 tctgaactaa ggacagaaaa aatctctcca acgtgatcat aatgctctgg aaggattttt
      181 tcaatggttt cgcgtccaag tggagtgaga gcaatcaagg aagcacgacg gtcatttgta
      241 tcgatagtct tgtagatata accatctcga atcatgttct ttataacgac agtcatgttg
      301 ccagaagtac ttaataattt atcaattaag tcttgaatcc gcagatttcc tttgttgtag
      361 agtgcctcca tcactccaaa ctgacaaact gtcagtccgt attttttgat tgtttctaat
      421 tctgattttg tgatagtatt agctgctctg cgaaagacga ctagactgtg aagtgcatgc
      481 ttgttctcgt ttaaagattg ttcaacagtt ctcatgagaa tattttagca ataaatagtt
      541 cggttatcaa gcaaaaaaag ttagatccta tattcatagg taaagtactt tatggaaaag
      601 atagttttta gttaatcaag ttcgtttttt gaggtaatac taactgtact ggaaaaaacg
      661 agcaatgagc atttgagaaa tagtcttaga tggaagtgca aaactgtgaa tgtaggtttt
      721 tatatccttt tctttgtgtg aaggaaaagg aaatggtaaa ttctcaaagc aagttctagt
      781 tcccgtcctt ccagaagtta ctttgagaaa actgcataac atcaacagaa tttagtctca
      841 gactaagttc tttttttgac taaaaatgtt gataatagtg agttttaggg ttgaaaatgt
      901 taaaaaaaaa agactacact aaagttactg gctggaaaag gaaattttat ttacaattac
      961 agaaagaatg gaaatatgct ttaatttgta gtataatggg actactatgg aaaaaaagat
     1021 ttttcaactg ttggagtgga tggcttccaa gactggtcag ttagtgttgg gctcttttat
     1081 ccttctcagt gttgtgactt tttcaatttt tactatttgg gatattgctg ctgccccttt
     1141 caacaatgcg cgtagtcatg cagttgcagt agctacagaa tatgctgatt tgcaaactgt
     1201 caatgatttt tctatctaca atggtactga aacctatttt tgtgtatttg gtgtcaccag
     1261 tcagggagaa gaggttgcag ttttgattcc agaggcttct agcacagtat atgtctatcc
     1321 tttagctcag ggaatttcgc aagaagaagc gcaagctatt gccaagaaaa acggtgcaag
     1381 ccaagtagag cgaactattc taggtttaag ggatggcaaa cctatttggg aagtcaagtc
     1441 agggactgcc tactatttag ttgaatttga aacagggaac tttatcaaac gggagggctt
     1501 atgagcaagt tatcaagacg agtcttagag atggaagaaa gtgtcacgct ggcagctggt
     1561 gcgcgtgcaa aagccttgaa ggcagaagga cgtgatattt tagagctgac cttgggtgag
     1621 ccagacttcg tcacaccgaa aaatattcaa gaagctgcta ttaagtctat cgaagatggt
     1681 aaggcgagtt tttatacggt agcttcaggt cttccagaat tgaaggacgc agtcaatacc
     1741 tactttgaga acttctacgg ctactctatc gaacgcaatc aagtcgttct tgccacaggt
     1801 gctaagtttg ttctctatgc cttctttgtg gcggtcatca atcctggcga tgaggttctg
     1861 attccaacgc cttactgggt atcctatgct gaccagatta agatgaatga aggtgtgcct
     1921 gtttttgtca ctgcgacgga agaacacaat tttaaggtaa cggttgacca gctggaagcg
     1981 gctcgtacgg ataagaccaa ggtattccta ctcaatagtc cttctaatcc gactggcatg
     2041 atttacagtc gtgaggaatt ggaagctatt ggaaactggg ctgtagagca cgatattctc
     2101 atcttggcag acgatattta tggtcgtttg gtctataatg gaaatacctt tacaccgatt
     2161 tcaagcattt cagaaagcat tcgccagcag actattgttg tcaatggggt ggccaaggtc
     2221 tatgccatga caggttggcg ggttggattt gctgtcggta atcctgaaat catagcggct
     2281 atgagcaaaa ttgtcggaca aacgacgtca aacttaacaa ccgctgctca gtatgcggcc
     2341 attgaagcct ttacaggtcc gcaagataca gtagaaacca tgcgtcaggc ctttgaagag
     2401 cgactcaata ccatttatcc cttgctggca gaagtaccag gttttgaagt gattaagcca
     2461 aaaggagcct tctatctctt cccgaatgtt aaaaaagcta tggaaatgaa aggctacaca
     2521 gatgtaactg aatttaccac agccattttg gaagaagtgg gagttgcaat ggtgacaggt
     2581 gctggttttg gagctcctga gaatatccgc ctcagctatg cgacagatat ggacacgctc
     2641 aaagaagcag tggctcgact acacacattt atgaaaaaat aaaaagagga aaaataatgt
     2701 ctagaaaatt gattaccatc aaccaagtaa aagattatgt cggtcaagaa gtgaccattg
     2761 gtgcctgggt tgccaacaaa tcaggcaagg gcaagttagc cttcctgcaa ttgcgtgacg
     2821 gaacagcctt cttccaagca gtggctttta agccaaactt catagaaaaa ttcggtgaag
     2881 aagctggtac tgagaagttc gatgtagcaa aacgtctcag tcaagaaacg tctgtctatg
     2941 tgacagggat tgtcaaggaa gatgagcgtt ctaagtttgg ttatgagttg gatgtgacag
     3001 accttgaagt gattggtgag tcacaagatt acccaatcac accaaaagaa cacggtacgg
     3061 acttccttat ggacaaccgt cacctctggt tgcgttctcg taaacaggtc gctatccaac
     3121 aaatccgtaa cgccatcatt tatgcgacct atgaattctt tgaaaagaat ggctttatca
     3181 aatttgatag cccaatcttg tctggaaatg cggcagaaga ttcaacagaa ttgttcgaaa
     3241 cagactactt tggtcaacca gcttatctca gtcaatcagg tcagctttac ctggaagctg
     3301 gtgctatggc tcttggtcgt gttttcgact tcggtcctgt tttccgtgcg gaaaaatcaa
     3361 aaactcgccg ccacttgacg gagttctgga tgatggatgc tgagtattca ttcctcagtc
     3421 atgaagagtc acttgacttg caagaagctt atgtcaaagc cttgattcaa ggtgttatcg
     3481 accgtgctcc acaagccttg gaaactcttg agcgtgatgt ggatgctctc aaacgctaca
     3541 ttgcggaacc attcaaacgt gtggcttatg atgatgccat tacccttctg caagaacatg
     3601 aagctgatga agatacagac tacgaacaca tcgagcatgg tgatgacttt ggatcaccac
     3661 atgaaacttg gatttcaaac tactttggtg taccgacttt cgttgttaac tatccagcaa
     3721 gcttcaaggc cttctacatg aagccagttc caggtaatcc agagcgcgtg ctttgtgcag
     3781 acctcttggc tccagaaggc tatggtgaaa tcatcggtgg ctctatgcgt gaggatgact
     3841 acgatgcttt ggtggctaag atggacgagc tcggcatgga taagtcagaa tacgacttct
     3901 accttgacct tcgtaaatac ggttcagtac cacacggtgg ttttggtatc ggtattgagc
     3961 gtatggtaac cttcgttgct ggtacaaaac acatccgtga agccattccg ttcccgagaa
     4021 tgttgcaccg cttgcatccg taatacagtc attcagtgtg tcttttatta cgttgttaac
     4081 tcgctttgcc gtacctcagt acagcctgca gctccttgcc tagtactaaa agtaaactga
     4141 aagactgcga aataaatatt tactgacaga ctacaaatgt ggtctgtttt cttgcttttt
     4201 cactgagttt tatttataat tattctaaat aagttgcaca gagagaaaaa atgaagcaag
     4261 aaagtttagg aaaacgtatt atcaaactag ccttgcctgc tacggtggaa aatatctttc
     4321 agaccttagt tggttttgtg gataccctct taatagccca gctagggctg gtagcggtga
     4381 cggcagttgg tcttgccaat acgattttga atgtctattt ggcggtct
//
LOCUS       DATUCG010000164         4262 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.166, whole genome shotgun sequence.
ACCESSION   DATUCG010000164 DATUCG010000000
VERSION     DATUCG010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4262
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.166"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(23..646)
                     /locus_tag="VCA20_002218"
     CDS             complement(23..646)
                     /locus_tag="VCA20_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HEP1835949.1"
                     /translation="MIFTYNKEHVGDVLMVFVAEDKGQPVQFERKGQVARVFQAETGQ
                     TVAWNIFEASSLVELTGNGQVFMTDEQVATLNAVLAEEGFAESLVNDNSPKFVVGQIA
                     ELVPHPDSDHLNICQVNVGDKTVQIVAGAPNAAQGLKTIVALPGAMMPSGSLIFPGKL
                     RGEDSFGMMCSPRELALPNAPQVRGIIELDDSTEVGEAFEPAKHWKG"
     gene            complement(666..1610)
                     /locus_tag="VCA20_002219"
     CDS             complement(666..1610)
                     /locus_tag="VCA20_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HEP1835950.1"
                     /translation="MKKSLGKILLAPVVLGLSLSLAPLVQADVINEKWGKPTLVYGGG
                     LSDAQATEVNSLFGILDVNNVSRQVVVGTDMDQYLGTSGADTASLYSSVLVQKQDAGK
                     GVVVDIKTPQNITLITETQYANAAITAGATDVLIDVAAPIQVTGESALTGVYKALATN
                     GETVDTARTEVAQQELTAVNEVATAHAGDEQFDSSALDKAVAEIKTALADYKKSNGQV
                     ASEADINTIINDVLAQNGLESVITADEISKLVTFAKAYQETSAIDSAEVAAQLNQFKQ
                     QAEQQISEAYKNLQDSGILEKIGAFFENLWKGLTGLFA"
     gene            complement(1655..1975)
                     /locus_tag="VCA20_002220"
     CDS             complement(1655..1975)
                     /locus_tag="VCA20_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEP1835951.1"
                     /translation="MIFPTNLEEVAALIEDGKPTVFLFVTTWCGDCHYIKPHLPAIEE
                     AFPDLRFVQLDRDDFMPLAQEWAILGIPSLVVLENGQEIGRFVDKNRKTKEEIMNFLS
                     GLGR"
     gene            complement(1972..2257)
                     /locus_tag="VCA20_002221"
                     /pseudo
     CDS             complement(1972..2257)
                     /locus_tag="VCA20_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637323.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
     gene            2404..3465
                     /gene="pepA"
                     /locus_tag="VCA20_002222"
     CDS             2404..3465
                     /gene="pepA"
                     /locus_tag="VCA20_002222"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEP1835952.1"
                     /translation="MTLFEKIKEVTELQSLPGFEGQVRNHIRQKITPHVDRIETDGLG
                     GIFGIKDTAVENAPRILVAAHMDEVGFMISQIKPDGTFRVVELGGWNPLVVSSQAFTL
                     QLQDGRTIPAISGSVPPHLSRGANAPGLPAIADIIFDAGFANYDEAWTFGVRPGDVLV
                     PKNETILTANGRNVISKAWDNRFGVLMVTELLESLSGQSLLNQLIAGANVQEEVGLRG
                     AHASTTKFNPDIFLAVDCSPAGDIYGDQGKIGDGTLLRFYDPGHIMLKNMKDFLLTTA
                     EEAGVKFQYYCGKGGTDAGAAHLKNHGVPSTTIGVCARYIHSHQTLYNMDDFLEAQAF
                     LQAIVKKLDRSTVDLIKNY"
     gene            complement(3786..3926)
                     /locus_tag="VCA20_002223"
     CDS             complement(3786..3926)
                     /locus_tag="VCA20_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HEP1835953.1"
                     /translation="MHQNLTERRSVLLTAFEVVQTILSFGGFTVALIGLCYKIFKDND
                     KK"
     gene            complement(4109..4243)
                     /locus_tag="VCA20_002224"
     CDS             complement(4109..4243)
                     /locus_tag="VCA20_002224"
                     /inference="COORDINATES: protein motif:HMM:NF012948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEP1835954.1"
                     /translation="MTYSRVERAKTLPNTGEQTSLLALVGVAVLSSLGLASARRRKQG
                     "
BASE COUNT         1153 a         1042 c          843 g         1224 t
ORIGIN      
        1 cacctgacca attcttcttt ttctatccct tccagtgttt agctggttca aaggcttctc
       61 cgacttctgt ggagtcgtcc aattcgataa tgccacggac ttgtggtgcg tttggtaatg
      121 ccaattcacg tgggctacac atcataccaa agctatcttc accacgcaac ttccctggga
      181 agattaggct accgctcggc atcattgccc ccggaagagc cacaatggtt ttcaatcctt
      241 gggcagcatt tggcgcacca gctacgattt gaacggtttt gtcaccgaca ttgacctgac
      301 agatattgag gtggtcgctg tctggatgag gaaccaattc cgcaatctga ccgactacga
      361 atttcggact attgtcgtta accaagcttt cagcaaagcc ttcttctgct aaaaccgcat
      421 tcaaggtcgc aacttgttcg tctgtcatga aaacctgacc attgccagta agctcaacca
      481 aacttgatgc ttcaaagatg ttccaagcga ctgtttgtcc cgtttctgct tggaagacac
      541 gtgctacctg gcccttgcgt tcaaactgga caggctgacc cttgtcttca gccacaaaca
      601 ccataagaac atcgccgacg tgttccttat tatatgtaaa aatcatttct tttttcattt
      661 ctcctttatg caaatagacc ggtcaaacct ttccaaagat tttcaaagaa ggctccgatt
      721 ttttctaaaa tccctgaatc ttgtagattc ttataggctt ctgaaatttg ttgctccgcc
      781 tgttgcttga actggttgag ttgggcagca acctcagctg aatcaatagc agaggtttcc
      841 tgataagctt tagcaaatgt gactagctta gaaatttcat ctgctgtgat gacactttcc
      901 aaaccgtttt gggccaaaac atcattgatg atggtgttga tgtcagcctc agaagccacc
      961 tgaccattgg actttttgta gtcggcaaga gcagtcttga tttctgctac tgccttatcc
     1021 aaagcagagc tgtcaaattg ttcatcgcca gcatgagctg tcgctacttc attaacagcc
     1081 gttaattctt gttgggctac ctctgtccga gctgtatcaa ctgtttcacc attggtcgca
     1141 agagccttgt aaacacctgt cagagcggac tctcccgtca cctgaatcgg tgccgcaacg
     1201 tcaatcaaga cgtctgtcgc accggcagta atggccgcat tggcatactg cgtttcagta
     1261 atcaaggtga tgttctgcgg tgtcttgatg tccaccacaa ccccttttcc agcgtcctgc
     1321 ttttgcacca agacagatga atagagggag gctgtgtccg ctccagatgt gcctaaatat
     1381 tggtccatat ccgttccaac aaccacctga cgactaacat tattgacatc caaaatacca
     1441 aataaactgt tgacctctgt cgcctgcgca tctgacaagc ctccaccata aaccagtgtt
     1501 ggcttacccc atttttcatt gatgacatcc gcttgaacta gcggtgccaa acttaaactc
     1561 aagcccagca ccacaggtgc taaaaggatt tttcctagtg acttcttcat gttctttcct
     1621 ttcttttggg aaatggcaga gcttatctgc taatttatct acccagtcca gataagaagt
     1681 tcatgatttc ttccttggtc ttgcgattct tatcgacaaa acgaccgatt tcctgaccat
     1741 tttccaaaac aaccagactt ggaatgccta aaatagccca ttcctgtgcc aaaggcataa
     1801 agtcatctcg atccagttgc acaaaacgga gatctggaaa agcctcttcg atagctggca
     1861 aatgcggttt gatatagtgg caatctccac accaagtcgt cacaaataga aaaacagttg
     1921 gcttgccatc ttcaatcaag gcagctactt cttcaagatt tgttggaaaa atcatctttc
     1981 ttcctctctg tattgaatag aaacctttgg tattttcaaa caaaaatacc tgtcctgcct
     2041 ccatcacaac ccctccgata agagtggtct gcgtagaagc cgtttcatcc acatagaccg
     2101 tggcaatctc tcctaaatca gcgaaaaagt cacgcacctc ctgcaaggcc tgtgcttttt
     2161 tacggtcttc ttggatttta cggtaaaatt gagcactagc ggtgagcaca cctgccccca
     2221 ataagcctgc tagcaaggct gtctttttct ttttcatgtt gactatttta tcatatttct
     2281 ccggattttt cccaaaaatg ccattggact tgtgacagct gagaagatcc ttttgacata
     2341 ttagtaggac aagtctagct cttcgtgata aaatagtagt aatcatgtca tggaggaaga
     2401 agaatgactc tttttgaaaa aattaaagaa gttaccgaat tacaaagttt accgggattt
     2461 gaaggacagg ttcgcaacca catccgccaa aaaatcacgc cacatgtaga ccgcatcgaa
     2521 acagatggac taggtggaat ttttggcatc aaggatacgg ctgttgaaaa tgctcctcgt
     2581 atcctagtgg ccgcccacat ggatgaggtt ggctttatga ttagccaaat caagccagac
     2641 ggtactttcc gtgttgttga gctaggtggc tggaatcctc tggttgtttc tagccaagcc
     2701 ttcactcttc agttgcagga cggtcgaacc attcctgcca tttcaggttc agtgccacct
     2761 cacctttctc gtggggctaa cgctcctggc ttgcctgcca ttgctgatat tattttcgat
     2821 gctggttttg ccaattatga cgaagcttgg acgttcggcg ttcgtccggg ggatgtcctt
     2881 gttcccaaaa atgaaacaat tctaacagcc aacggaagaa atgttatttc taaggcttgg
     2941 gacaaccgct tcggtgtcct tatggtgacg gaattgctgg aaagcctatc cggtcaatca
     3001 ttgctaaatc agttgattgc tggagccaat gtgcaagagg aggttggtct acgtggagct
     3061 catgcttcta caaccaaatt caatccagac attttccttg ccgttgattg ctcgccagct
     3121 ggagatattt atggcgacca aggaaaaatt ggtgacggaa ctctgcttcg tttctatgat
     3181 ccaggtcata tcatgttaaa aaacatgaaa gacttcctcc tcacaactgc cgaagaagca
     3241 ggtgtcaaat tccaatacta ctgtggtaaa ggtggaacag atgccggtgc agcccacttg
     3301 aaaaaccatg gcgttccatc gacaaccatc ggcgtctgcg ctcgttacat ccactctcac
     3361 caaaccctct acaatatgga tgacttctta gaagcccaag ccttcttgca ggctatcgtt
     3421 aaaaaactag accgctcaac ggttgatttg atcaagaatt attagacagt tatgcaaagc
     3481 gaagaagtcc cctatgatcg ctgagtctac ccaactagta aaaaataaca tagcagtaaa
     3541 cgctctctaa cctatatcgt catattctgg cattcaccga taagtctgag cctactataa
     3601 gaaacaaacc tatagcaaac gaatcaccaa agttaatata gcgtttgcat tttacaagca
     3661 atctgatata atgaaaatag aaaaagagct ggacggcaat ccagctccga atgtagaacc
     3721 gttaaaaaga cggtggctcg tattacagat ttaatccgtc ctactcgcgc aaaagtgggg
     3781 acgggttatt ttttgtcatt atctttgaag attttatagc acaaaccaat caaagcaact
     3841 gtgaaaccac cgaaacttaa aatcgtctgc acaacttcaa aagctgtcaa aagaacgctc
     3901 ctcctttccg tcagattttg atgaattgcc cataggcatc accttacttt cagaaaatca
     3961 agagctaccg tcttcacttt tctactcatc tagtacatca tatttcattc ctaaaaattg
     4021 ttggaactag cgtagtcttt ccgaaacttt taggaatcca aaaaatcttc ctactgtcat
     4081 agtaagaaga ttttttcatt ttagccatct atccttgttt gcgtcttctt gcacttgcaa
     4141 gaccaaggct agacaataca gccacaccta cgagtgccaa caaacttgtt tgctcccctg
     4201 tgtttggcaa ggtcttagca cgttctacac gtgagtaagt cactttttct ccagtagctg
     4261 tt
//
LOCUS       DATUCG010000165         4250 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.167, whole genome shotgun sequence.
ACCESSION   DATUCG010000165 DATUCG010000000
VERSION     DATUCG010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4250
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.167"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            594..2585
                     /locus_tag="VCA20_002225"
     CDS             594..2585
                     /locus_tag="VCA20_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HEP1835955.1"
                     /translation="MGKKLKFSLVAVACATFLTACGTSNSSAPKAGEATPQTDFHSYI
                     NAQWLKDTKLGVGESQIDNLSQLSEKTQEAIQEIIDQLDMDYSKLQAGSDEKKLIDFY
                     RLAADFDTRNKLGLEPIKPYLEEIKKASTMEELNKILVALYGKNIRNLLHINVTQDIK
                     DSNKNTIYVDPHELSFPKENYDGTDEFSLKNQKAFKAYLQEVFSLSGETSKEASSKAD
                     LLFNLEKEMAAVQRPKEEANNFEAMYNEKTWDEVKALVPNLPIAELVSELGLQNAPTT
                     VVSDPDALKKLNELYQEKNLDALKALLQYRVIAHYNAYLSSNLIEAKAKYDGVATGAV
                     DTPSQKEVAQAAIDANFSDMIGKVYVKHHFSEESKANILSMVQEIKATYAERIKAVQW
                     MSEETKMRALKKLDTLVVKIGYPDQWKDYSQLAIQSKEEGGSLLSNFDMVKTLELREE
                     MGQFGKPFDKSQFGMPSHTVNAYYSPTNNEIVFPAGILQAPLYDPKASPEENLGGIGA
                     VIGHEISHAFDKAGSQFDEKGNLVNWWKPEDLEKFNKKVQQAADIYSKLEVAPGYYVN
                     GEISTGEIMADLGGLTVAIDIAQKKGYDTKKVFESYGKAWREVTTKEFAIANISDEHP
                     PAKYRVNNIVNQIDQFYTDFNVKEGDPMYVKPEDRLRVW"
     gene            2952..3962
                     /gene="gap"
                     /locus_tag="VCA20_002226"
     CDS             2952..3962
                     /gene="gap"
                     /locus_tag="VCA20_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEP1835956.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPVMLAHL
                     LKYDTTQGRFDGTVEVKDGGFEVNGKFVKVSAEREPGNIDWATDGVDIVLEATGFFAS
                     KEKAEQHIHANGAKKVVITAPGGNDVKTVVFNTNHDILDGTETVISGASCTTNCLAPM
                     AKALHDAFGVQKGLMTTIHGYTGDQMVLDGPHRGGDLRRARAAAANIVPNSTGAAKAI
                     GLVIPELNGKLDGAAQRVPVPTGSVTELVATLDKKVTAEEVNAAMKAAATESFGYTED
                     QLVSSDIVGISFGSLFDATQTKVIEVDGEQLVKVVSWYDNEMSYTAQLVRTLEYFAKI
                     AK"
     gene            complement(4115..>4250)
                     /locus_tag="VCA20_002227"
     CDS             complement(4115..>4250)
                     /locus_tag="VCA20_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEP1835957.1"
                     /translation="EQGHRLLPLPPYSPEYNPIEKTWAHIKKHLRRVLPNCDTFLEVL
                     "
BASE COUNT         1372 a          783 c          909 g         1186 t
ORIGIN      
        1 gtagcttaga cttgctattt ttgattttta ttgagtataa aaacaaacta aacgactaaa
       61 aaataggtat tcgtcagatc ctttttggaa atatacaatg agaaagctac aatttataaa
      121 aaattcccct aatttaggtg tggatggcta acaaaagatc agggtttgct attttgggat
      181 ttcattgggt ataagttcct gttgtcgatg aactcaacag tgactcccag gttcgactac
      241 tgtgaaataa acggattaaa acagtctagg gttagactat ttcagctcaa caactagaaa
      301 ttaggaattg ttgccgaact cttttgatcg ttcgagttct cgcctactca cacttaatca
      361 gctaaaataa atgttttaaa ttatttagaa ttactttgtt tgggaatggt tataaaaaat
      421 aagagaatta atagataggc tacagaagaa aagcagatat aaatgttaga atccttatga
      481 ggtagatcga tgaaagagta gtttaggacc gctaattact aaaggaggat gcttgtatgg
      541 tgatgaaata gtggtactaa gcacattaac aataaaaaga aaggacagaa accatgggaa
      601 aaaaactaaa attttcatta gtagcagtcg cctgtgcgac atttttgaca gcctgtggca
      661 caagtaattc ctcggctccc aaagcaggtg aagcaacacc acagacggat tttcattctt
      721 atatcaatgc gcaatggttg aaagatacaa aattaggtgt cggtgaatcg caaatagata
      781 atctgagtca actctctgag aaaacgcaag aagcgataca ggagataatt gaccagctag
      841 atatggacta ttccaagctg caagctggta gtgatgagaa aaaacttatt gatttttacc
      901 gattggcagc cgattttgat actcgcaaca aattggggtt agagcctatt aagccttatc
      961 tagaggagat taaaaaagcc tctaccatgg aagaactcaa caagattctg gtggccctat
     1021 acggcaaaaa catccgcaat ttgcttcata taaacgttac tcaagatata aaagacagta
     1081 acaaaaacac catctacgtt gaccctcacg agttaagttt tccaaaagaa aattatgatg
     1141 gcacagatga attttcattg aaaaatcaaa aagcctttaa agcctatctg caggaagtgt
     1201 ttagcttgtc aggagaaacc tcaaaagaag cctcttctaa ggcagatctc ctctttaatt
     1261 tggaaaaaga gatggctgca gtgcaacggc caaaagaaga agccaataac tttgaagcca
     1321 tgtataacga gaagacttgg gatgaggtca aagcactggt tcccaaccta ccgattgctg
     1381 agttggtttc tgagttagga cttcagaatg ccccaacgac agtcgtatca gatccagatg
     1441 ctctaaaaaa attaaatgaa ctctatcaag aaaagaattt agacgctctt aaggctctgc
     1501 tccaatatcg tgtgattgcc cattacaatg cttatctgtc aagcaattta atcgaggcaa
     1561 aagcgaaata cgacggcgta gcaaccggtg cggtagatac tccgagtcag aaagaagttg
     1621 ctcaagcggc cattgatgca aatttctccg atatgattgg caaagtttac gttaaacacc
     1681 atttttcaga agaatccaag gctaatatcc tatctatggt tcaagagatt aaagcgactt
     1741 atgcagagcg aatcaaagca gtgcagtgga tgtctgaaga aaccaagatg cgggccttga
     1801 aaaaactaga tacattggtt gttaaaatcg gctatccaga ccagtggaag gattactcac
     1861 aacttgctat ccaatccaag gaagaagggg ggagtcttct ttctaacttc gatatggtaa
     1921 aaacacttga attgcgtgaa gagatggggc aatttggcaa gccttttgat aagtcgcagt
     1981 tcggcatgcc ttctcacacg gtgaatgcct attacagccc aactaacaac gaaattgtct
     2041 ttccagcggg gattttacag gctcctctct acgatccaaa agctagtccc gaagaaaatc
     2101 taggtggaat cggggctgtt atcggtcatg aaatttccca tgcctttgat aaggctggtt
     2161 ctcaatttga tgaaaagggg aaccttgtca actggtggaa accagaagac cttgaaaaat
     2221 ttaataaaaa ggttcaacag gcagcagata tctactctaa attggaagtg gctccgggct
     2281 actatgtcaa tggcgaaatt tcaaccggtg aaatcatggc ggatctcggt ggtttaacgg
     2341 ttgccattga tattgctcag aaaaaaggct acgatacgaa gaaagtcttc gagtcttatg
     2401 gtaaagcatg gcgtgaagtc accaccaaag aatttgctat tgccaacatc agcgacgaac
     2461 atccaccagc taaatatcgg gttaacaaca ttgtcaacca aattgatcaa ttctatacag
     2521 acttcaatgt caaagaaggg gatccaatgt atgtgaaacc agaagaccgc ctacgagtgt
     2581 ggtaaaagat tatagaggtg tctgcggtcc aaaatgggtg gtagacacct ttttaggtaa
     2641 aaaatagaag tagtagtaaa ccaagaatcg gtaaaaaatg tgtcgaggta tggcaatgcc
     2701 attaagttaa gtatttaacc agtcgtaacc acccgagaaa acgcatggct atgattttta
     2761 gcttttattt tttaatcttc tattttttac ataagatgaa ccggtaagca gttgcaattt
     2821 tcggtaaata gttatataat aaaaacgttg aaaagatgct tgtaggctta caagttaata
     2881 ttttcacaaa aggattaaaa ggcttgcctt ttaaaataaa gaactcgatt ttcataagga
     2941 ggaaatcatt catggtagtt aaagttggta ttaacggttt cggacgtatc ggtcgtcttg
     3001 ctttccgtcg tatccaaaac gtagaaggtg ttgaagttac tcgtatcaac gaccttacag
     3061 atccagtaat gcttgcacac ttgttgaaat atgacacaac tcaaggtcgt ttcgacggta
     3121 ctgttgaagt taaagacggt ggtttcgaag ttaacggtaa attcgttaaa gtttctgctg
     3181 agcgtgagcc aggaaacatc gactgggcta ctgatggcgt agatatcgtt ttggaagcta
     3241 caggtttctt tgcttctaaa gaaaaagctg agcaacacat ccacgctaac ggtgctaaaa
     3301 aagttgttat cactgctcct ggtggtaacg atgttaagac tgttgttttc aacactaacc
     3361 acgatatcct tgatggtact gaaacagtta tctcaggtgc ttcatgtact acaaactgtt
     3421 tggcaccaat ggctaaagct cttcacgatg catttggcgt tcaaaaaggt ttgatgacta
     3481 caatccatgg ttacactggt gaccaaatgg ttcttgacgg accacaccgt ggtggtgacc
     3541 ttcgtcgtgc acgtgctgct gctgcaaaca tcgttcctaa ctcaactggt gctgctaaag
     3601 ctatcggctt ggtaatccca gaattgaacg gtaaacttga cggtgccgca caacgtgttc
     3661 cagttccaac aggttctgta actgaattgg ttgcaactct tgacaagaaa gtaactgctg
     3721 aagaagtaaa cgctgctatg aaagctgctg ctactgaatc atttggttac actgaagacc
     3781 aactcgtatc ttcagatatc gtaggtatct cattcggttc attgtttgat gcaactcaaa
     3841 ctaaagttat cgaagttgat ggcgagcaat tggttaaagt tgtttcatgg tacgacaacg
     3901 aaatgtctta cactgcacaa cttgttcgta ctcttgagta cttcgcaaaa atcgctaaat
     3961 aatccttagt gaaaatcgaa gaggcttcgg cctctttttt gttttgattg ctgaaatata
     4021 gctgtttcct tatgctaaga agcaaggata gcataagttg tttctgattt ggcgatttta
     4081 tactcattta tagtcaactg aaacaagagc aggactaaag tacctcaaga aacgtatcgc
     4141 aattcggcaa tactcttctg aggtgttttt tgatatgagc ccatgttttc tcgataggat
     4201 tatactctgg cgagtaagga ggaagtggta acagtctatg tccctgctcc
//
LOCUS       DATUCG010000166         4239 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.168, whole genome shotgun sequence.
ACCESSION   DATUCG010000166 DATUCG010000000
VERSION     DATUCG010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4239
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.168"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            116..1906
                     /gene="dnaG"
                     /locus_tag="VCA20_002228"
     CDS             116..1906
                     /gene="dnaG"
                     /locus_tag="VCA20_002228"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEP1835958.1"
                     /translation="MLSKDKITEIKQALNIVDVIGETVALTKAGRNYVGLCPFHGEKT
                     PSFNVIEDKQFYHCFGCGKSGDVFKFVEEVRGVSFADAAAILAEKAGFQVDVTPSYHQ
                     EEKRVSPHQVLYDIHLDAAKFYHALLMTTKMGEEARAYLHQRGLTDDVIKTFQLGLAP
                     AEQNILYQKLSGQYDEESLLNSGLFNPSENNIIYDAFQARIIFPLADEYGRIVAFSGR
                     IWTEEDRNNKQLAKYKNSRSTAIFNKSYELYHLDKAKAVIKKQRETYLMEGFLDVIAA
                     HRVGIDNAVASMGTALTREHVAHLAKFCKKIVLTYDGDKAGQAATMKALDELQDFQVE
                     IVSLPDNMDPDEFLQKNSEEALQQVLTKSRISDVEFLIQYLKPENPDNLQMQIEFVDK
                     IAPIIAKVSSITAQNSYIYKVADLLSDFDYNQVEQAVNAVRLNQRQERTQQVFQSQTG
                     QSYLSPVQTIARITSLIRTENHLLYRMVEHPYILNEFRLREDFYFATPELQAIYDMLK
                     KYGEITSFELSQLNDSAQQAWYRMQEERLPNEVSPHEIEELEIRRDKELLKKENQHLA
                     RKIREHSHVGNADIALGELQKLLDRRRQME"
     gene            1909..3027
                     /gene="rpoD"
                     /locus_tag="VCA20_002229"
     CDS             1909..3027
                     /gene="rpoD"
                     /locus_tag="VCA20_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEP1835959.1"
                     /translation="MTNKKDKKTEVTTFDVQVAEFIRNHKKQGSATDDEINDQLVIPF
                     TLDADGIDDLLQRIQDAGISIVDKEGNPSARAMQVEEEPELSDEELLGSTSAKVNDPV
                     RMYLKEIGVVPLLTNEEEQELALAVEAGDPEAKQRLAEANLRLVVSIAKRYVGRGMQF
                     LDLIQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVET
                     INKLVREQRNLLQELGQDPTPEQIAERMDMTPEKVREILKIAQEPVSLETPIGEEDDS
                     HLGDFIEDEVIENPVDYTTRVVLREQLDEVLDTLTDREENVLRLRFGLDDGKMRTLED
                     VGKVFNVTRERIRQIEAKALRKLRHPSRSKPLRDFIED"
     gene            3610..3933
                     /locus_tag="VCA20_002230"
     CDS             3610..3933
                     /locus_tag="VCA20_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEP1835960.1"
                     /translation="MTYTEDQIKDIQERIFHALEDVIDPELGIDIINLGLIYEIRFIE
                     GKAEIDMTLTTMGCPLADLITDQIHDVLKDVSEVTEVDVRLVWSPAWTVQKMSRYARI
                     ALGIK"
BASE COUNT         1327 a          788 c          946 g         1178 t
ORIGIN      
        1 agcaatttgt tttagaaatt ttacattttc ccgtatttag tatacaatag agtatgttaa
       61 actaagggaa gagtgttcac tttttaaatt agctagtatg atatgaggag ggaatgtgct
      121 ttcaaaggat aaaattactg aaataaaaca agctctcaat atcgttgatg ttattggtga
      181 aacggttgct ttgactaagg ccggacgtaa ttatgtcggt ctttgccctt ttcatgggga
      241 gaaaaccccg tctttcaatg tgattgaaga taagcagttc taccattgtt ttggatgtgg
      301 taagtccgga gatgtattca agtttgtcga agaagttcgt ggtgtttctt ttgcagatgc
      361 ggcagctatc ttagctgaga aggcagggtt tcaagtagat gtaacccctt cctatcatca
      421 agaggagaaa agggttagtc cgcatcaggt actgtatgat attcatctgg atgctgcaaa
      481 gttttatcat gcccttttga tgacaaccaa aatgggtgag gaagctagag cttatctaca
      541 ccaacgtggc ttgacagatg atgtcataaa aacattccag ctaggcttag ctcctgccga
      601 acagaatatt ctttaccaaa aactatcagg tcaatatgac gaagaaagtc tattgaattc
      661 gggtttgttc aatccaagtg agaataatat tatttatgac gcctttcaag cgaggattat
      721 tttccccttg gcggatgaat atggtcgaat tgtagctttt tcaggtcgga tttggaccga
      781 agaagatcga aacaataaac aattagctaa gtataaaaac tctcgtagta ctgcgatttt
      841 caataaaagc tatgaattgt atcatttgga caaggctaag gcggttatta agaagcaacg
      901 tgaaacctat ctcatggaag gttttctaga tgttattgct gctcatcgtg tagggattga
      961 taatgcagtg gcttccatgg gaacagcttt gacaagagag catgttgcac acctagctaa
     1021 gttttgtaag aaaattgttc tgacttatga tggggataag gctggacagg cagctaccat
     1081 gaaggcgttg gacgaactac aagattttca ggttgaaatt gtgagtttgc cagataatat
     1141 ggacccagat gaatttttgc agaaaaattc agaagaagcc ttgcaacagg ttttaactaa
     1201 gtcacgaatc agcgatgtag aatttttgat tcaatacttg aaacctgaaa atcctgacaa
     1261 tctacaaatg cagattgagt ttgtagataa gattgcacca attattgcca aagtatcatc
     1321 cattacagct caaaattctt atatttataa ggtggcggat ttactttctg actttgatta
     1381 taatcaggtg gaacaggcag taaatgctgt tcgtctgaac cagcgccagg agagaacaca
     1441 acaggtattt caatcacaga ctggtcagtc ttatttgtca ccagtccaaa cgattgctcg
     1501 tataacgagt cttattcgaa cagaaaacca tttactttat cggatggttg aacatcctta
     1561 tattctcaat gagttccgtt tacgagaaga tttttatttt gcgacgccag aattacaagc
     1621 tatttatgat atgttaaaaa aatatgggga aattaccagt tttgaactat cacagttgaa
     1681 cgattccgct caacaggctt ggtatcgtat gcaggaagaa cgtttaccta atgaagtatc
     1741 tccacacgaa atcgaagaat tagaaattcg gcgagacaag gaattgttga agaaggagaa
     1801 ccagcacctg gcacggaaaa tccgtgaaca ttcccatgtg ggaaatgcag atattgccct
     1861 cggtgagttg caaaaactcc tcgatagaag aagacaaatg gagtaaatat gacaaataaa
     1921 aaagataaaa aaacagaagt aactactttt gatgtacaag ttgccgaatt tatccgtaac
     1981 cataaaaaac aaggttcagc tacagacgat gaaatcaatg accaactggt tattccattt
     2041 acgcttgatg ctgacggaat cgatgatttg ctccaacgta ttcaggacgc agggatttcg
     2101 attgttgata aagaagggaa cccttctgct cgtgctatgc aggtagagga agaaccagaa
     2161 ttatctgatg aagaattgct cggaagtact tctgccaaag ttaatgaccc tgtccgcatg
     2221 tacttgaaag aaattggtgt tgtacctctt ttgaccaacg aggaagagca agaattggca
     2281 ttggcggttg aggcaggtga cccagaagcg aaacaacgtt tggctgaagc caacttgcgt
     2341 ttggttgtat ccattgccaa acgctatgtc ggccgtggta tgcagttcct tgatcttatc
     2401 caagaaggaa acatgggctt gatgaaggcc gttgacaagt tcgactactc taaaggtttc
     2461 aaattttcaa cctatgcaac ctggtggatt cgtcaggcta tcactcgtgc tattgcggac
     2521 caagcgcgta caatccgtat ccctgttcac atggtggaga ctattaataa attggtccgt
     2581 gagcaacgca atctcttgca ggaattgggc caagatccaa ctccagagca aatcgctgag
     2641 cgtatggata tgacgcctga gaaagttcgt gaaatcctca agattgccca agagcctgtt
     2701 tcactcgaga ccccaattgg tgaagaagat gatagtcatc ttggagattt tatcgaagac
     2761 gaagtcattg aaaatccagt tgattacaca actcgtgtcg ttcttcgtga acagttagat
     2821 gaggtattgg atactcttac tgaccgtgag gaaaacgtat tgcgtcttcg ttttggttta
     2881 gatgatggta aaatgcgtac gcttgaagat gttggtaaag tctttaacgt aacccgtgaa
     2941 cgcatccgac aaatcgaagc caaagccctc cgtaaactcc gccacccatc ccgtagcaaa
     3001 ccactcagag attttattga ggattagaaa ggtactgcat acctttctaa cgcctgccca
     3061 gaaataagaa agcaggctaa cccagtggtg ttttaacccg agcctagaaa taagaaagcg
     3121 aggttaacct ccgagggagg tttttaaccg ctcgctgaaa actgggaacg agcgttctca
     3181 acgtccgcct aaaattccga aggcggacta agtccagtgg actacaagga tcctgacttc
     3241 cgtcagtcct atttcccacc ttcaacagtc tcccagactg ttgaagcctg cccagaaata
     3301 agaaagcagg ttaacccagt ggtgttttaa cccgagccta gaaataagaa agcgaggcta
     3361 atcttcgggg gaggtgatcg acgtttctca tatcattcga ccataatgat gtgagaaact
     3421 agtaagtcgt ctaggcaatt ctattgggaa ttgccgttag ttgagtagct tttcaaagca
     3481 ctcaactttg gcgagttgaa ccgctcccct tgaaattaga aagggagcgt aacgtccagt
     3541 ggactttttg acacaaactt ggtttgtttc ttttctagta ttgaacttgt gaacaagaaa
     3601 gagagaaaaa tgacgtacac agaagatcaa attaaagaca ttcaggagcg tatttttcat
     3661 gctcttgaag atgtgattga ccctgagcta ggtattgata ttatcaattt agggctaatt
     3721 tatgaaattc gatttattga gggcaaggct gaaattgata tgaccttgac gaccatgggt
     3781 tgtccattag ctgatctaat taccgatcaa attcatgatg tactgaagga tgtttctgaa
     3841 gtaacagaag tagatgtgag actggtttgg tcaccagctt ggaccgtgca aaaaatgagt
     3901 cgttatgctc gaattgcatt aggaattaaa taaaaaacaa aatggtttcg aacgtttgaa
     3961 gccatttttt atgtgacttt tagtccagtc cgtcacgctc cgtaaggtgc gtgacaaata
     4021 aaccacccgc tatgtgggtg cgcgacgcgc gatacaataa aggtgttcaa gccaattgta
     4081 aagcgagagg agaaaagtat ggcacaaaag gcacatagtt tatcacatac caaatggatg
     4141 tgcaaatatc acatagtttt cacaccaaag taccacagaa aatccattta ctacaaaatt
     4201 agacaggact tatacttaac aaaaatcata aatagcgat
//
LOCUS       DATUCG010000167         4216 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.169, whole genome shotgun sequence.
ACCESSION   DATUCG010000167 DATUCG010000000
VERSION     DATUCG010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4216
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.169"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(254..418)
                     /locus_tag="VCA20_002231"
     CDS             complement(254..418)
                     /locus_tag="VCA20_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835961.1"
                     /translation="MGDKPISFKDKDGNFVSAADVWNAEKLEELFNKLNPNRKLRLER
                     ECLARKSENE"
     gene            complement(553..1866)
                     /gene="obgE"
                     /locus_tag="VCA20_002232"
     CDS             complement(553..1866)
                     /gene="obgE"
                     /locus_tag="VCA20_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEP1835962.1"
                     /translation="MSMFLDTAKIKVKAGKGGDGMVAFRREKYVPNGGPWGGDGGHGG
                     KVVFVVDEGLRTLMDFRYNRRFKADDGEKGMTKGMHGRGAEDLIVRVPQGTTVRDADT
                     GKIITDLVENGQEFVIAHGGRGGRGNIRFATPKNPAPEISENGEPGEERNLELELKVL
                     ADVGLVGFPSVGKSTLLSVITAAKPKIGAYHFTTIVPNLGMVRTKSGESFAVADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVLDMSASEGRDPYEDYVAINNELETYNLRLME
                     RPQIIVANKMDMPEAAENLEEFKKKLAANYDEFEELPQIFPISGIAHQGLENLLDATA
                     ELLEKTPEFLLYDESDFQEEEAYYGFNPDEPEFAISRADDASWILSGDKLEKLFTMTN
                     FDRDESVMKFARQLRGMGVDEALRARGAKDGDIVRIDKFEFEFVD"
     gene            complement(2071..2199)
                     /locus_tag="VCA20_002233"
     CDS             complement(2071..2199)
                     /locus_tag="VCA20_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEP1835963.1"
                     /translation="MAFGEEKHKKTTFEKVTLVIVVVMVVATLAGLILPAINALMN"
     gene            complement(2256..4040)
                     /gene="uvrC"
                     /locus_tag="VCA20_002234"
     CDS             complement(2256..4040)
                     /gene="uvrC"
                     /locus_tag="VCA20_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEP1835964.1"
                     /translation="MNDLIKHKLELLPDSPGCYLHKNKYGKIIYVGKAKKLRNRVRSY
                     FRGAHDTKTEALVSEIADFEFIVTDSNIEALLLEINLIQENKPRYNIMLKDDKSYPFI
                     KITKERHPRLLITRQIKKDGGQYFGPYPDVGAANQTLKLLERLYPFRKCKLPENKYCL
                     YYHLGQCLAHGENLPSLSDYDKMAKEVAQFLTGHDDKIVKEVQDKMKAAAGNLEFEKA
                     AEYRDVLQSISTLRTKQRVMAHDLQNRDVFGYYVDKGWMCVQVFFVRQGKLIERNVNL
                     FPYYNDADEDFLTYIGQFYRDQQHLIPSEVLIPQDIDQEAVEALVDTKVIKPQRGEKK
                     QLINLATKNARVSLEQKFNLLEKNLEKTYGAVENIGQLLGIPTPVRIEAFDNSNIMGT
                     NPVSAMVVFENGVPNKKEYRKYKIKTVEGPDDYASMREVLQRRYSRVLRDGLTPPDLI
                     IIDGGQGQVNIAKQVIEQELGMSIPIAGLQKNDKHQTHELLFGTPLEVVELPRNSQEF
                     FLLHRIQDEVHRFAIEFHRQVRSKNSFSSKLDGIEGLGPKRKQALMKHFKSIGNIQSA
                     SLQEIAEAGVPYKVAELVKESLSSKDEI"
BASE COUNT         1169 a         1044 c          781 g         1222 t
ORIGIN      
        1 aatcatccat tttccgcaac atacgcaaaa aaaacgcatg taactttttt ctagttgcat
       61 gcccattctg atattgtgga ttggttacct acaggtcaat ggttctgacg cgagtcctat
      121 cttactctct ataggcgtgg atcacaattg tgccacttgg tcattgagta taagattgct
      181 acagcacaaa caatcatatt tttaaaattc ttaataaaaa cctgctagtc ttttgaaact
      241 aacaggtctc taattattca ttttccgatt ttcttgccaa acactcgcgt tccaaacgga
      301 gcttgcggtt gggattgagc ttattaaaaa gttcttctaa tttctcagca ttccatacat
      361 cggctgctga gacaaaattt ccatccttat ccttgaagga tattggttta tcgcccatat
      421 aaacctccaa ctattttata gactatatac aaacgatcat taactgaatg aaattagtaa
      481 ggtagctagc caagatccca taggacttgg cgtagtttga ctagcaagtt aacttgcagt
      541 caaactgcta cgttaatcaa caaactcaaa ttcgaacttg tcaatacgga cgatgtctcc
      601 gtctttggca ccacgcgcac ggagggcttc atcgacaccc attccacgca actggcgagc
      661 aaatttcatg acagattcgt cgcgatcgaa gttggtcatg gtgaagagtt tttccagctt
      721 gtcaccagat agaatccaac ttgcatcgtc tgcacgactg attgcaaatt ctggctcatc
      781 tgggttaaag ccatagtaag cctcttcttc ttggaaatct gattcgtcgt agagcaagaa
      841 ttctggcgtt ttctctagca actcagctgt ggcatccaaa agattttcca aaccttggtg
      901 agcaatccct gagattggga aaatctgtgg caattcttca aactcatcat agttggctgc
      961 taatttcttc ttgaactctt ccagattttc tgccgcttct ggcatatcca ttttgttggc
     1021 aacgataatt tgagggcgct ccattaaacg aagattatag gtttccaatt cattgttgat
     1081 ggctacatag tcctcataag gatcacgacc ttcacttgct gacatatcca aaacatgcaa
     1141 gataacacgt gtccgctcaa tatgacgtag gaattgcgtt cccaatccaa caccttgact
     1201 agcaccctca atcaaacctg gtaagtctgc cacagcaaat gactcgccag atttggtcct
     1261 caccattcct aagtttggaa caatggttgt aaagtgatag gcaccaatct taggtttagc
     1321 agctgtaatc acgctaagaa gcgttgactt accaactgac ggaaaaccaa caagacctac
     1381 atcagccaac actttcaatt ccaattcaag attgcgctcc tcacctggtt ctccattttc
     1441 agaaatctca ggcgctggat tcttaggtgt cgcaaaacga atatttccac gaccaccacg
     1501 accaccgtgg gcaatgacaa attcctgccc attctcaact aggtctgtaa tgatttttcc
     1561 tgtatcagcg tcacgcacag ttgttccctg cggtacacga acaattaaat cttccgctcc
     1621 tcgaccgtgc atacctttag tcatcccttt ttcaccatca tcagccttaa agcgacgatt
     1681 gtaacggaag tccatcaagg tccgcaagcc ttcatctaca acgaagacaa cttttccacc
     1741 atgaccacca tcaccgcccc aaggaccgcc gtttggtaca tacttttcac gacggaaggc
     1801 aaccatccca tcgcctccct tacctgcctt gaccttaatc ttggcggtat ctaaaaacat
     1861 actcataatt taccctattc tttcctagaa aaaaacgctt aaaagcgttt taaacctcta
     1921 ttcacaagcg aagtgcttat atataagaga agttttttag ctaatcaagg tcgttctttg
     1981 agagaatact gactgtgttt tgaaaaaact aacgatgaac agcttaaaaa ccaaccttag
     2041 ataaaagtgc cgaactgtaa atacaggttc ttaattcatc aatgcattaa tagctggtaa
     2101 aatcaagcca gccaatgtcg cgacaaccat cacaacaacg attactaaag tcactttctc
     2161 aaatgttgtt tttttgtgtt tttcttcacc aaaagccatt gctttctcct tatttagtca
     2221 ttgatagttt attctaacac attatgggaa tgttttcaaa tctcatcctt tgaagaaagg
     2281 ctttccttga ccaactccgc caccttatac ggcactcctg cttctgcgat ttcttgtaga
     2341 ctggccgact ggatattgcc aatagacttg aaatgcttca tcaaagcctg cttgcgtttt
     2401 ggtcccaagc cttctatgcc gtccaatttt gatgagaagg aatttttaga acgaacctga
     2461 cggtgaaact caatagcaaa gcggtgcact tcatcttgaa tccgatgcag gaggaaaaat
     2521 tcctgcgaat tacggggtaa ctccaccact tccagtggag taccaaaaag caattcgtgg
     2581 gtctggtgct tgtcattctt ctgcaaacct gcaattggta tagacatgcc taactcctgc
     2641 tcgatgacct gcttggcaat gttaacctgc ccctgaccac cgtcaatgat aatcaaatca
     2701 ggcggtgtca aaccgtcccg cagtacccga ctgtaccgcc gctgcaaaac ttcccgcata
     2761 gaagcatagt catccggtcc ctccaccgtc ttaatcttat acttgcggta ttctttttta
     2821 ttaggcaccc cattttcaaa gaccaccatg gccgatacag gattggtccc cataatgttg
     2881 gagttatcga aagcctcaat ccgcacaggc gtcggaatcc ccaaaagctg accgatattt
     2941 tctacagcac cgtaggtctt ctccagattt ttctccaaaa gattaaactt ctgctccaga
     3001 ctaacacgcg cattcttggt agccagattg atgagttgct tcttctcgcc acgctggggc
     3061 ttgatgacct tggtatctac cagagcttct acggcttctt ggtcaatatc ctgcggtatc
     3121 aagacctctg agggaatcaa atgttgctgg tccctgtaaa actgaccgat gtaggtcaaa
     3181 aaatcttcgt ccgcatcatt atagtatggg aagagattga cattccgctc aatcagtttc
     3241 ccctgccgaa caaagaaaac ctgcacacac atccagccct tgtccacata gtagccaaag
     3301 acatcgcggt tttgcaagtc atgagccatc actcgttgct tggtccgtag ggttgaaatg
     3361 gattgcagga catctcggta ttccgcagcc ttttcaaatt ccaaattgcc agctgccgcc
     3421 ttcatcttgt cctgaacttc cttgacaatc ttatcatcat gccccgtcag aaattgggca
     3481 acttcttttg ccatcttgtc atagtcagac aggctgggca ggttttcccc gtgtgccaaa
     3541 cattgcccca aatgataata aagacaatac ttgttctcag gcaatttaca cttgcggaaa
     3601 ggatagaggc gctccaatag tttgagggtc tggttggccg cacccacatc tggataggga
     3661 ccaaaatact gaccgccatc cttcttaatc tgccgtgtaa tgagcagccg tgggtgacgt
     3721 tctttagtaa ttttgataaa aggataagac ttgtcatcct tgagcatgat gttatagcga
     3781 ggcttatttt cctgaatcag attgatttcc aacaagagcg cctcgatatt agaatccgtt
     3841 acgatgaatt caaagtcagc aatctctgac accaaggcct ctgtcttggt atcatgggca
     3901 ccacgaaaat aagaccgcac ccgattgcgc agttttttgg ccttgcccac atagataatc
     3961 ttaccgtact tgtttttatg aaggtagcaa cccggactgt ccggcagcaa ctccaactta
     4021 tgcttgatta aatcgttcat aggtctatta tagcaaattt tctgataatc gagcttattg
     4081 cattcctttt tacactcgac ttcttggaat atatccgcat ccatactaca aaaatatagt
     4141 ggcttgaatt aggaaaagga cagccttaag ttatagtgtt aataacccac tcctaaaata
     4201 actttagagt tctcaa
//
LOCUS       DATUCG010000168         4121 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.170, whole genome shotgun sequence.
ACCESSION   DATUCG010000168 DATUCG010000000
VERSION     DATUCG010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4121
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.170"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            26..1117
                     /gene="serC"
                     /locus_tag="VCA20_002235"
     CDS             26..1117
                     /gene="serC"
                     /locus_tag="VCA20_002235"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HEP1835965.1"
                     /translation="MTIYNFSAGPAVLPKPVLERAQAEFLDYNGSGMSVLEMSHRSKD
                     FDDIIKGAEATLRELMAIPDNYKVLFLQGGASLEFTMIPLNFAQGKKAYYLVGGSWGK
                     KAYTEAVKLSKTIAFEPILLGSTEDITYAELPTFDKNYIDPTAAYVHLTTNNTIEGTA
                     VYDIPDTNGVPVIADMSSNILAARYNVEDFAMIYAGAQKNIGPAGVTVVIVREDFLND
                     QPMLSSMLDYRIQAENDSLYNTPPAYSIYISKLVFEWVKEIGGVDEMEKINHEKSGLL
                     YDYIDQSNFYTNPVRKKEERSVANIPFVSPSEELDAKFVKEATAAGFKNIKGHRSVGG
                     MRASLYNAFPRQGVVELIEFMKKFAVENA"
     gene            1117..1674
                     /locus_tag="VCA20_002236"
     CDS             1117..1674
                     /locus_tag="VCA20_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEP1835966.1"
                     /translation="MEIRLAHPNEMEAIVDILEDARAFLATSGINQWQGEYPNKENIF
                     DDILSGRGYVGLVDGKVAAYAAVHRGQEAEYEAIYEGKWQHNHPVYTTFHRIAAADEF
                     RAQGIIQTFLQGLIEGQKGPDFRCDTHEQNKTMQHILKKLGYVYCGKVPIDGERLAYQ
                     KIKHKSERSLYQEISEDDRWLLGNN"
     gene            2048..3229
                     /locus_tag="VCA20_002237"
     CDS             2048..3229
                     /locus_tag="VCA20_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEP1835967.1"
                     /translation="MVFSVRTFNNINQIGLKELGNKFQIDGDHAANPDAFIIRSENLH
                     GFDFPKNLKAIARAGAGTNNIPIEEATEKGIVVFNTPGANANAVKEAVIASILLSARD
                     YIGATAWANTLSGDDVPKQVEAGKKQFAGTEISGKTLGVIGLGAIGARIANDARRLGM
                     NVLGYDPYVSVETAWTISSHVKRVDDLKEIFTNADYITVHVPLTDKTRGLFNADSFGQ
                     MRKGTTLINFARGELVNNADLFEAIEAGVIKNYITDFGTEEVLNKDNIIVFPHVGGST
                     EEAELNCAIAAGQTIRRFMETGEIINSVNFPNVKQFLDAPYRITLINKNIPNMVATIT
                     TAVSELGINIDNIINKSKGDYAYTLLDLDEADKVKIEQLVAKFEATDAIVKVRVIQNK
                     N"
     gene            3232..3726
                     /locus_tag="VCA20_002238"
     CDS             3232..3726
                     /locus_tag="VCA20_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEP1835968.1"
                     /translation="MLYKQIYHSPLGPMSLMASDKGLRGAWFEGQKYFERGLNEKAIL
                     ASHPILDQTARLLDKYFSGEQVDFSNLPLEAVGTNFQEHVWQLLKTIPIGQTTTYGQL
                     AQQLGLRSGQAVGGAVGRNPYSIIVPCHRVLNQKGQLTGYAGGLDKKIWLLRHENPQF
                     EVKK"
     gene            3723..4076
                     /locus_tag="VCA20_002239"
     CDS             3723..4076
                     /locus_tag="VCA20_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEP1835969.1"
                     /translation="MILFYEYPKCSTCRAAKAELKSLGLEFEAIDIKLTPPSAEELKS
                     WMGATGLELKKYFNTSGNSYRELGLKDKFDSLTVDQALDLLANDGMLIKRPLLIQDGK
                     ILQIGYRTKYENLGL"
BASE COUNT         1259 a          758 c          942 g         1162 t
ORIGIN      
        1 cagactaact taatggaggg attttatgac aatctacaac ttctctgcag gtcctgcagt
       61 attgccaaaa ccagtacttg aacgcgccca agctgaattc ttggactaca atggttcggg
      121 aatgagtgtt ttggagatgt cccatcgctc caaggacttc gatgatatta ttaaaggtgc
      181 tgaagcgacc ctgcgtgaac ttatggctat tcctgataac tacaaggtgt tgttcttaca
      241 aggtggagca tctttggaat tcacaatgat tcccctcaac ttcgctcaag gcaagaaagc
      301 ctactatctt gtaggtggat catggggtaa aaaagcctat actgaggctg tgaagctgtc
      361 taaaacaatt gcctttgaac caattttgct tggatcaacg gaagatatta cctatgcaga
      421 attgccaacc ttcgataaaa attatattga tccaactgct gcttacgtgc atttgacaac
      481 aaataacact attgaaggaa cagcagttta tgatattcct gacaccaatg gtgtgccagt
      541 cattgctgat atgtcttcaa acatcttagc ggctcgctat aatgttgaag attttgctat
      601 gatttatgct ggtgctcaga aaaatattgg ccctgctggt gtgacggtgg tgattgttcg
      661 tgaggacttc ctcaatgatc aaccaatgct ttcaagcatg ttggactacc gtattcaagc
      721 agaaaatgac tctctttata atacgccgcc agcttattct atctatattt ctaaactcgt
      781 ctttgaatgg gtaaaagaaa ttggtggtgt cgatgagatg gagaaaatca accatgaaaa
      841 atctggcttg ctttatgact atatcgacca gtctaatttc tatacaaacc ctgttcgtaa
      901 aaaagaagag cgttcagtag cgaacattcc ttttgtctca ccaagtgaag aattagatgc
      961 taaatttgtc aaagaagcaa cagcagctgg ttttaaaaac atcaaaggtc accgttcagt
     1021 aggtgggatg cgggcatcgc tctataatgc tttcccacgt caaggtgtgg ttgaattgat
     1081 tgaattcatg aaaaaatttg cagtggagaa tgcttaatgg aaattagatt agcacatcca
     1141 aatgagatgg aggctattgt cgatattctt gaagatgctc gtgcatttct agcaacctcc
     1201 ggtatcaatc aatggcaggg agagtatcct aataaagaga atatctttga tgacattctt
     1261 tctggcagag gttacgttgg tctagttgac ggtaaggtgg cagcttatgc cgccgttcat
     1321 cgtggtcaag aagcagaata tgaggccata tatgaaggaa aatggcagca caatcatcct
     1381 gtatatacaa catttcatag aattgccgca gctgacgaat ttcgtgctca aggaatcata
     1441 caaacttttt tgcaaggttt aattgaaggt caaaaaggac cggattttcg ctgtgatacc
     1501 cacgagcaga ataagacaat gcagcacatt ctaaaaaaat taggttatgt ctattgtggt
     1561 aaagtcccaa tagatggaga acgtctagcc tatcaaaaaa tcaagcacaa gagcgagcgg
     1621 agtctttacc aggaaatcag tgaagacgac cgttggttgc tcggaaataa ttaatgaaat
     1681 aaagaaagaa aaggaccggg caaaagcttc atatcacttc tcaaggttcg ggtcaacatc
     1741 tcagcgcagt ggttgattgg gtttaacagt ccagtggact gttaaaggtt ggagataaga
     1801 tttgcgaagc aaatctcagc agttcgtgtt ttacactcca aatctgacca ctacaactga
     1861 tgcgaacaga gttcgcctta tctccaagct caaactgtct cccagacagt ttgagctgtg
     1921 cgggggtggg agtgaaacag tctgggaata gactgtttca gctcaacaaa tagaaataaa
     1981 gacttgttga cgaactcttc taggagtggt tgagttcttt cccacttccc tttttaggta
     2041 aaaataaatg gtatttagcg tacgtacttt taacaatatc aatcaaattg gtcttaagga
     2101 attgggcaat aaattccaga ttgacggaga tcatgcagca aatccagatg cctttattat
     2161 tcgatctgaa aatttgcatg gatttgattt tcctaaaaat ctaaaagcta ttgcccgtgc
     2221 tggtgcaggg actaataata ttccaatcga agaggcaaca gagaaaggta ttgttgtctt
     2281 taacactcca ggtgccaatg ccaatgccgt aaaagaggca gtcattgctt ccatcctctt
     2341 gtcagcacgt gactatatcg gagcgactgc atgggcaaat acattgtcag gagatgatgt
     2401 acctaagcag gttgaagcag gtaagaagca gtttgctggg acagaaattt caggtaagac
     2461 tcttggtgtt atcggacttg gtgctatcgg tgcgcgtatt gccaacgatg cccgccgttt
     2521 gggaatgaat gttctcggtt acgaccctta tgtatcagta gaaactgcat ggaccatttc
     2581 aagccatgtc aaacgtgtgg atgacttgaa agaaatcttt accaatgcgg attacattac
     2641 tgttcatgtt cctttgacag ataagacacg aggacttttc aatgcggata gctttggtca
     2701 aatgagaaaa ggaacaacct tgataaactt tgcccgcggg gaactggtta ataatgctga
     2761 tttgtttgaa gcaattgagg caggagttat caagaactat atcacggatt ttgggacaga
     2821 agaggtgctc aacaaggaca acattatcgt cttcccgcac gttggtgggt caacagagga
     2881 agcagagctc aactgtgcca ttgctgcagg tcagaccatt cgtcgtttta tggagacggg
     2941 tgaaattatc aactcagtta acttcccaaa tgtgaaacaa ttcctagatg caccgtatcg
     3001 tattaccttg attaataaga atattccgaa tatggtggca acgatcacaa cggctgtatc
     3061 tgagttggga atcaatatcg ataatatcat caataagtct aaaggtgact atgcctatac
     3121 tttacttgat ttggatgaag cggataaagt gaaaattgag cagttggttg ctaaatttga
     3181 agcaacggat gcaattgtca aagtgcgtgt cattcagaat aaaaactaat catgctgtat
     3241 aaacaaatct atcacagtcc acttggcccc atgtcattaa tggctagtga taagggattg
     3301 agaggggcct ggtttgaggg acaaaaatat tttgaacgag gcttgaatga gaaagctatt
     3361 ttagcctccc atcccatttt agaccagaca gctcgattgc ttgacaagta tttttcaggg
     3421 gaacaggtgg acttttcaaa ccttccatta gaagctgtgg gaacaaattt ccaagagcat
     3481 gtctggcaat tattgaagac gattccgatt ggtcagacga ctacctatgg acaactggct
     3541 cagcaactgg gccttcgttc tggtcaggca gtaggaggag cagttgggcg taatccttat
     3601 tctattattg ttccttgtca ccgagttctc aatcagaaag gacagttgac aggttatgca
     3661 ggtggactgg ataagaaaat ctggctcttg cgacatgaaa atccacagtt tgaggtgaag
     3721 aaatgatctt attttatgaa tatcctaaat gttcgacttg ccgagcagca aaggcggagc
     3781 tgaagagttt gggattggaa tttgaagcta ttgacattaa gttaactccg cctagtgctg
     3841 aagagctcaa atcttggatg ggggcgacag gactagaatt gaaaaagtat ttcaatactt
     3901 caggaaatag ttaccgcgaa cttggtctga aggataaatt tgacagtttg acggtggatc
     3961 aagcattaga cttattagct aatgatggta tgctgattaa gcgtccccta ctaattcagg
     4021 atggtaaaat cttacagatt ggctatcgaa caaaatacga aaatctcggt ttataaggtc
     4081 gggatttttt attgagtata aaagtctaaa taaaaggtaa t
//
LOCUS       DATUCG010000169         4103 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.171, whole genome shotgun sequence.
ACCESSION   DATUCG010000169 DATUCG010000000
VERSION     DATUCG010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4103
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.171"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            128..1078
                     /locus_tag="VCA20_002240"
     CDS             128..1078
                     /locus_tag="VCA20_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEP1835970.1"
                     /translation="MNNTGIHHISSLVGNIHQAYHFYHHILGLKLTLKTVNQEDSSMY
                     HLFFGDEEGRYGTEFTIFDMPNHPNHRNGTNRLERTVFLVKNFAALEFWQKRLTEFEV
                     ENEGIQAFGNGHILNFQDEDGQLLGLTYHELVGETFPYKTDEIPSEYAILGIASLHMR
                     IREEKELLSLLTETFGFVEESRFVFEDKMVISLLFDSTFQHQLYLILDQESPISVMGV
                     GAIHHIAFGVLDESDLEDLISRLNLINRPHSGIINRDFIHSLYFRAPNYLMFEVATMA
                     GEREATMPRQDKQLDKVELFLPSFFEEDRQEIEKNLSQRY"
     gene            1301..1693
                     /locus_tag="VCA20_002241"
     CDS             1301..1693
                     /locus_tag="VCA20_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835971.1"
                     /translation="MRLSAFLDFWQDLDIQPYQQITFVGFSNGINFIMGLLTKQSNLA
                     YNYILLHPSALDYAFPMENSRAEILFTLGQNDQLVDQVALKNLVDDWQASAFPRANLA
                     YFDKGHFLSQDELTYVRNWYQEKINEKT"
     gene            complement(1720..3510)
                     /gene="pepF"
                     /locus_tag="VCA20_002242"
     CDS             complement(1720..3510)
                     /gene="pepF"
                     /locus_tag="VCA20_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEP1835972.1"
                     /translation="MTNAMPKRQEIDVQLTWDTDILFPTPDNYKENLATYAKQVTAFE
                     SNYKGKLTDKDAIVSALTEYEKIVILDSRLSHYAFLPLEVDKMNTELASLANEYDLVS
                     AKARPQRDFLYSELGQLDEAFLLELKEEQPQWAAFFDSILRDKPHQLHPLQEELLSNF
                     APTFGQPYNNYGVTKFEDMTFDNFEANGETLGNSYVLFENDYELSHDTEIRRNSAAGF
                     YSTLKKYKNTTAATYLSHIKNEQIEARLRGFDNTIDFLLHSQNVSRDLFDRQIDVIMK
                     ELAPHMRRYVKLVAKAHGLDKITHADLKISLPSEYNQRITPKESKQFLIDCLGILGEE
                     YVKMIEQSFDERWIDFAQNEGKATGGFCATLYDGPSYILLSWTGLMNEVLVLAHELGH
                     AGHFQLAKKQSVLSYEPSLYFIEAPSTANEVLTCNTLLKNNQDPGFQAYLISELISRT
                     YFHNMVTHLLEAAFQREVYTRLDNDEYLNGDILCQIKLDIIKEFWGEDFEIGDDAGLI
                     WMRQPHYYIGLYPYTYSAGLTIGTAMAKQLEEHPEEVVEKWLETLSLGASLSAQDLAK
                     HAGVDVSTDQPLKETIAYVGSLVDKLESLI"
     gene            3677..4015
                     /locus_tag="VCA20_002243"
     CDS             3677..4015
                     /locus_tag="VCA20_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002264292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835973.1"
                     /translation="MQVIKRSGEIVEFDPDKIYQAIIKAAQTVYVIDETWRKNLAQVT
                     KKVVLDLEEAHTERPTISMVQSQVEHRLLDAGYISIAEHYISYRLQRDLERNGYGDKI
                     IVHLRFEQVR"
BASE COUNT         1205 a          781 c          879 g         1238 t
ORIGIN      
        1 tatccttctc taatcagcaa tggttaggga agttttttat tacttggctg tcagtcgtgc
       61 gcaaaagatt tttatttatc aataattagc gataaaataa gtctatcaac atcagtgagg
      121 tcatcttatg aacaatacag gtattcatca catttcaagc ttggtaggaa atattcatca
      181 agcctatcat ttttatcatc atattctcgg cttaaaattg accttgaaaa ctgtcaatca
      241 agaagattca agcatgtatc atctcttttt cggtgatgaa gagggccgct atggaacgga
      301 atttaccatt tttgatatgc ctaaccatcc caatcatcga aacggaacca atcgtttgga
      361 aagaactgtt tttctggtaa aaaattttgc tgccttggaa ttctggcaga aaagactgac
      421 agagtttgaa gtggaaaacg aaggaattca ggcttttgga aatgggcata ttctgaattt
      481 ccaagatgaa gatggtcagc tgcttggctt gacttaccat gaattagtcg gagaaacgtt
      541 tccgtataaa acagatgaaa ttccgtcaga atacgctatt cttggcatcg ccagtctaca
      601 tatgcggatt cgtgaagaaa aggaattgtt atctttgctg acagaaacct tcggatttgt
      661 tgaggaaagt cgatttgttt ttgaggataa gatggtgatt tctcttctct ttgacagcac
      721 cttccaacat caactttatt tgatacttga ccaagagtcg ccaatcagcg ttatgggagt
      781 cggggccatt catcatatcg catttggtgt cttagatgag tccgacttgg aagatctgat
      841 tagtcgctta aatctcatca atcgtccaca ttctggtatc attaatcgag atttcattca
      901 ttccttgtac ttccgagcgc ccaattatct catgtttgag gtcgcgacca tggctggcga
      961 aagagaggct accatgccaa gacaagataa gcagctagat aaagttgaac tgttcttgcc
     1021 aagtttcttt gaagaggaca gacaggaaat cgaaaaaaat ctctcccaac gttattagga
     1081 gaaatgagat atggattatc gctttatcaa agggacaagt ccccgtcttt tggtcttttt
     1141 ccacggaaca ggtggtaaca aggaatccat gctctttctc cgccaacagc tggatccaaa
     1201 agcttctgtc ctttccttgg atggaagttg gggtcaaggc agggaaaggc atttctttgc
     1261 accgctgatc gatggtcagt tggattttgt cgatgttgaa atgcgtttgt cggcttttct
     1321 ggatttttgg caggacttag atattcagcc ctaccagcag attacctttg ttggcttttc
     1381 aaacggtatc aactttatca tgggcttgtt aaccaagcag tcaaacctgg cgtacaatta
     1441 tatcttactc catccctcag ccttagatta tgcatttcct atggaaaata gcagagcaga
     1501 gatattgttt acactggggc agaatgacca actcgtcgac caagtcgctc ttaaaaatct
     1561 tgtagatgac tggcaagcgt cagcttttcc cagagctaac ttagcttatt ttgacaaagg
     1621 acactttcta agtcaggacg aattgaccta tgtgagaaat tggtatcagg aaaaaataaa
     1681 tgaaaagacc tgaacaaatg ttcaagcctt taaaagttat tagattaagg attctaattt
     1741 gtcaaccaat gaaccgacat atgcgatggt ttccttgaga ggttgatctg ttgaaacgtc
     1801 aacacctgcg tgtttcgcaa ggtcttgggc agaaagactt gcaccgagtg agagggtttc
     1861 caaccatttt tcaacaactt cttcaggatg ttcttccaat tgcttagcca tagcagtacc
     1921 gatggtcaaa ccagctgagt aggtatatgg atacaaacca atgtagtagt gtggttgacg
     1981 catccagata agacctgcat cgtcaccgat ttcaaagtct tcaccccaga attccttgat
     2041 gatgtctagc ttgatttgac agagaatgtc cccatttaga tattcatcat tatcaaggcg
     2101 agtgtagact tcacgttgga aggcggcttc aagcaagtga gtcaccatgt tgtggaagta
     2161 ggtgcgacta atcaattcac taatcaaata ggcttggaaa ccaggatctt gattgttttt
     2221 caagagggta ttacatgtca agacctcatt tgcagtagat ggcgcttcga tgaagtacag
     2281 tgatggttca tagcttagca cactttgttt cttagccaat tggaaatgac cagcatgtcc
     2341 taattcatga gccaatacca ggacctcatt catcaaacct gtccatgaaa gtaagatgta
     2401 agacggtcca tcatacaagg tagcacagaa tccacctgtt gccttgcctt cgttttgagc
     2461 aaagtcaatc caacgctcat caaatgattg ttcaatcatt ttcacatatt cttcaccaag
     2521 aatacccaag cagtcaatca agaattgttt ggattcttta ggagtaatgc gttggttata
     2581 ttcagaaggc agactgattt tcaagtcagc atgagtgatt ttatccaaac catgagcctt
     2641 agcaaccaat ttcacatagc gacgcatgtg aggggccaat tctttcatga tgacatcaat
     2701 ctgacggtcg aagaggtcgc gagagacatt ttggctatgc aagaggaagt caatcgtatt
     2761 gtcaaatcca cgcaagcgag cttcgatttg ttcgtttttg atatgagaca aataagtcgc
     2821 tgcagttgta ttcttgtatt tcttcaaggt tgaatagaag ccagcagctg aattgcgacg
     2881 gatttcagta tcatggctga gttcataatc attttcaaag agaacatagc tgtttccaag
     2941 tgtttcacca ttggcttcaa aattatcaaa ggtcatatct tcaaacttgg tcacaccgta
     3001 gttgttatat ggctgaccaa aagttggggc gaagtttgag agaagttctt cttgaagtgg
     3061 gtgaagttga tgaggtttgt cacgaagaat gctgtcgaaa aatgcagccc attgtggttg
     3121 ttcctctttt aattcaagta gaaaggcttc atctaattgc ccaagttctg aatagaggaa
     3181 atctcgttga ggacgtgcct tggcagaaac gaggtcgtat tcgttggcca ggctcgccaa
     3241 ttcagtattc atcttatcga cttccaaagg aaggaaggca tagtgggaaa gtctgctatc
     3301 aagaataacg attttttcgt attctgttaa ggcggaaacg atggcatcct tatcagttag
     3361 cttccctttg taattcgatt cgaaagctgt cacttgctta gcgtaggttg ctaaattttc
     3421 tttgtagttg tcaggggtcg gaaataaaat gtccgtatcc caggttaatt gcacatcaat
     3481 ttcttgacgt ttaggcattg catttgtcat gattttctcc ttatttcatt ttaataggtc
     3541 cattatatca tactaaaggg ctttcagcga aaataagcca aggcaaaatt tatgaaaaag
     3601 aatacttgac aaaatacaca aaaacctaga taatgtaagt atgaaagggt ttactgaaaa
     3661 ccagaaagga gcttttatgc aagtcattaa acgtagtgga gagattgttg aatttgaccc
     3721 agataaaatc taccaagcca tcattaaagc tgcacagaca gtctatgtca ttgatgaaac
     3781 ttggcggaaa aatcttgctc aggtcacaaa gaaagtagtg cttgacttgg aagaagctca
     3841 tacagagcga ccaacgatta gcatggttca gtcgcaagtt gaacaccgtc ttttagatgc
     3901 agggtatatt tctattgctg agcattacat ttcctatcgc ttgcaacgtg atttagaacg
     3961 taacggctat ggcgataaga ttattgtaca tctgcgtttt gaacaagtac gttaagagaa
     4021 actggctctt tgtcaactgt agtgggtaga cgaaaagcta acacctagag aggatgaaat
     4081 tcgttctctc atttttgatg ttt
//
LOCUS       DATUCG010000170         4064 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.172, whole genome shotgun sequence.
ACCESSION   DATUCG010000170 DATUCG010000000
VERSION     DATUCG010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.172"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(26..676)
                     /locus_tag="VCA20_002244"
     CDS             complement(26..676)
                     /locus_tag="VCA20_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEP1835974.1"
                     /translation="MYQTILFDLDGTLTDSGQGILNSVAYALEKMGIEEPDAANLNRF
                     IGPPLYESFSRFYQLSPEDTQSAVDAFRVYFKEKGMFENQLYPGIIPLLEELRTAGKT
                     LVIATSKPEIFAKQILEHFGIAHYFDIIAGASLDSSRLSKADVIGYAINQLEAFPKHG
                     VMIGDREHDIEGARRHQLPAIGVLYGYGSKQEFEKAGATMIVETIQDLKRILLTTE"
     gene            784..1911
                     /locus_tag="VCA20_002245"
     CDS             784..1911
                     /locus_tag="VCA20_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEP1835975.1"
                     /translation="MHILIAPDSFKESLSATKVAEAIKKGFSKVYPQAGYDLIPLGDG
                     GEGTVESLMQALSLDGTQTWVTGPFGDKIKVSYAVKDDLAVFEMAEIVGLASIPHERR
                     SPLFIKTQGVGELIVELVQNGVKHIFIGVGGSASHDGGIGMAAGLGVKFYNRERKEVE
                     AIGAHIGEIVSFSTEDMLVDLSQVRIDLVTDVENPLCGPAGATFVFAGQKGLASAEFE
                     LVDKKMESFYKLVDPMLLDLAGAGAGGGMAAGLVQFTGARIQKGIDFVLDQLDFDYRV
                     AKADLVVVGEGRMDAQSLSGKTPIGVARRTPTGIPIIAICGSLKDDLPEFPFENICAA
                     FPIIASVESLEDTFQKAEKNLVRTAEQVARILQLGGQQRWN"
     gene            1902..2132
                     /locus_tag="VCA20_002246"
     CDS             1902..2132
                     /locus_tag="VCA20_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835976.1"
                     /translation="MELVKYVAIILFIFICLAKLVSVMRLSEKERGTEMNVIKANGAM
                     FFFNEKNERGRLLGILCLTCIIFALIFILLYR"
     gene            complement(2139..2591)
                     /locus_tag="VCA20_002247"
     CDS             complement(2139..2591)
                     /locus_tag="VCA20_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1694 domain-containing protein"
                     /protein_id="HEP1835977.1"
                     /translation="MNDLNTTILQKASGETRLNPDEQRLYMGTYRERVVLIVSFDDLS
                     GEVVGNKFDTICQKLANSYSPLFLKLSPALSDKQQISLLKIAQNFGITTAIIDEKIGQ
                     SPYALVFHTNHAVDIEEVSLESTFPNLISKPEEKKEDVKPSFWKKLFG"
     gene            2741..4048
                     /gene="eno"
                     /locus_tag="VCA20_002248"
     CDS             2741..4048
                     /gene="eno"
                     /locus_tag="VCA20_002248"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEP1835978.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYLGLGTQKAVDNVNNVIADAIIGFDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYTYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMILPVGAPSFKEGLRWGAEVFHALKKILKARGLVTAVGDEGGFAPKFEGTEDG
                     VETIIEAIEAAGYEAGENGIMIGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQI
                     DYLEELVNKYPIITIEDGMDENDWDGWKALTERLGKRVQLVGDDFFVTNTDYLARGIK
                     EGAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAG
                     QIKTGSLSRTDRIAKYNQLLRIEDQLGEVAVYKGLNSFYNLKK"
BASE COUNT         1141 a          766 c          948 g         1209 t
ORIGIN      
        1 cattggctga gtgctggctt ttcttctatt ctgttgtcag taaaatcctt ttcaaatctt
       61 ggatggtttc gactatcatg gtggccccag ccttttcaaa ttcctgcttg ctcccatagc
      121 cgtaaagaac accgatggcg ggaagttggt gcctgcgtgc tccttcaatg tcatgttccc
      181 tatctccaat catcacacca tgttttggaa atgcttctaa ttgatttata gcatagccga
      241 tgacgtccgc cttactgagg cgactactat ccagactagc accggcaatg atatcaaagt
      301 agtgagcgat accaaaatgt tctagaatct gtttggcaaa tatttctggc tttgaggtag
      361 caattactag tgtttttcca gccgttctca attcttcaag cagagggatg attccaggat
      421 agagttgatt ttcaaacatt cctttttctt taaaatagac acggaaagca tcaactgcac
      481 tttgtgtatc ttcagggctg agttggtaga atcgtgaaaa agattcatag agtggcgggc
      541 cgatgaatcg gtttaaattg gctgcatctg gttcttcgat tcccattttt tctagtgcgt
      601 aggcgacgga gtttaagatt ccttgtccag aatcagtaag tgttccatct aagtcaaata
      661 atatagtttg atacatactt atctcctttc tttcagtata acacagacga tgaaaaacag
      721 aaaagattta ttgaaagctg aataggagcg tgatatacta ataatataaa ggaggttagt
      781 accatgcata ttctcattgc tccagattca tttaaagagt ctctttcagc tacaaaggtt
      841 gctgaagcga ttaaaaaagg attttctaag gtttacccac aagcagggta tgacttaatt
      901 cccctaggag atggtggcga agggacagtt gagagtctaa tgcaggcctt atccttggat
      961 ggaacccaga cttgggtgac aggtccattt ggagataaaa ttaaggtttc ctatgcggtt
     1021 aaagatgatc ttgcggtttt tgaaatggct gagattgtag ggctggcaag tatcccgcat
     1081 gaaagaagga gtcctctctt tattaagaca caaggggttg gggagctgat tgtggagctc
     1141 gttcaaaatg gtgtcaaaca catatttata ggagtaggtg gctctgccag tcatgatggt
     1201 ggtatcggaa tggcggcagg tttaggagtg aaattttata atcgagagag aaaagaagta
     1261 gaggcaattg gagctcatat cggggagatt gtcagctttt ccacagagga tatgttggta
     1321 gatctatctc aggtcaggat tgatttggta acggatgtgg agaatccgct ttgcggacca
     1381 gcgggtgcca catttgtttt tgcaggtcag aaaggtcttg cctctgctga atttgaacta
     1441 gtagacaaga aaatggagtc cttttataaa ttagttgatc caatgctact cgatttagca
     1501 ggagcgggtg ctggaggagg tatggcagca ggtctagtcc aatttacagg tgctcgcatt
     1561 caaaaaggca tcgactttgt cttggatcag ttggactttg attatcgcgt cgccaaagcg
     1621 gatctggttg tggtcggaga agggcggatg gatgctcaaa gcttatcagg gaagactccg
     1681 ataggagtag ctagacggac gccgacgggg attcccatca ttgcaatttg tggtagcctc
     1741 aaagacgatc ttcccgagtt tccttttgaa aatatctgcg ccgcttttcc gataattgca
     1801 agcgttgaaa gtttggagga cacgtttcaa aaagcagaaa aaaacctggt ccgcacggca
     1861 gagcaggtgg caagaattct tcagttagga ggccaacaaa gatggaatta gtgaaatatg
     1921 tggctattat cttatttatt tttatttgtc ttgcaaagct agtatccgtt atgagacttt
     1981 ccgaaaaaga aagaggaacg gaaatgaatg taataaaggc aaacggagca atgttctttt
     2041 ttaatgaaaa aaatgaaaga ggcagattgc taggaatcct ttgtctaacc tgtattattt
     2101 ttgctttaat atttatactg ctttatcgat aaataagtct aaccaaataa ttttttccag
     2161 aaggaaggtt ttacatcttc ctttttttct tctggttttg agataagatt tggaaatgtg
     2221 gattctaagc taacttcttc aatatccaca gcgtgatttg tgtgaaagac aagtgcgtag
     2281 ggagattgcc ctattttttc atcgatgata gcggtagtaa tgccgaagtt ctgagcaatt
     2341 tttagtagtg aaatttgttg cttgtcagac agggctggcg agagtttgag gaatagagga
     2401 gagtagctgt ttgctagttt ttggcaaatt gtatcgaact tatttccgac gacttcacca
     2461 ctcaaatcgt caaaggaaac gatcaggaca acccgttcgc gataggttcc catgtataga
     2521 cgttgttcat ctggattcaa tcgtgtttcg ccagaggctt tctgtagtat ggtagtattt
     2581 aaatcgttca tggtttcagt ataacatact cagttaactt tgaaaaggaa ggcgtttaca
     2641 ttatttcttt cagtaaaata acctgttttt tcttgaaaaa tcccttcttt tagggtatga
     2701 tagctatgta acaattttaa actcaaagga gagtaatata atgtcaatta ttactgatgt
     2761 ttacgctcgc gaagtccttg actcacgcgg taaccctaca cttgaagttg aagtttatac
     2821 tgaatcaggt gctttcggac gtggtatggt tccttcagga gcttctactg gtgagcacga
     2881 agcagttgag cttcgcgacg gtgacaaatc tcgttacctt ggtcttggta ctcaaaaagc
     2941 tgttgacaac gtgaacaacg tgattgctga cgctatcatc ggttttgacg ttcgtgatca
     3001 acaagctatc gaccgcgcta tgatcgctct tgacggtact cctaacaaag gtaaattggg
     3061 tgcaaacgca atcctcggtg tttctatcgc tgttgcgcgt gctgctgctg actaccttga
     3121 agtgccactt tacacttacc ttggtggatt caacactaaa gtattgccaa ctccaatgat
     3181 gaacatcatc aacggtggtt ctcactcaga cgctccaatc gctttccaag aattcatgat
     3241 cttgccagtt ggcgctcctt cattcaaaga aggtcttcgt tggggtgctg aagtattcca
     3301 cgctttgaag aaaatcttga aagctcgtgg tttggtaaca gctgttggtg acgaaggtgg
     3361 tttcgctcct aagttcgaag gaactgaaga cggtgttgaa acaatcatcg aagctatcga
     3421 agctgctggt tacgaagctg gcgaaaacgg catcatgatc ggtttcgact gtgcgtcatc
     3481 tgagttctac gacaaagagc gtaaagttta cgactacact aaattcgaag gtgaaggcgc
     3541 tgctgttcgt acatctgcag aacaaatcga ctaccttgaa gaattggtta acaaataccc
     3601 aatcatcact atcgaagatg gtatggatga aaacgactgg gatggctgga aagctcttac
     3661 tgagcgtctt ggcaaacgcg ttcaattggt tggtgacgac ttcttcgtaa caaacactga
     3721 ctaccttgca cgtggtatca aagaaggtgc tgctaactca atccttatca aagttaacca
     3781 aatcggtact cttactgaaa catttgaagc tatcgaaatg gctaaagaag ctggctacac
     3841 tgccgttgta tcacaccgtt caggtgaaac tgaagattca acaatcgctg acatcgcagt
     3901 tgcaactaac gcaggtcaaa tcaagactgg ttcattgtca cgtacagacc gtatcgctaa
     3961 atacaaccaa ttgcttcgta tcgaagatca acttggtgaa gttgcagtct acaaaggctt
     4021 gaactcattc tacaacttga aaaaataaat tagtacagtg tact
//
LOCUS       DATUCG010000171         4049 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.173, whole genome shotgun sequence.
ACCESSION   DATUCG010000171 DATUCG010000000
VERSION     DATUCG010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4049
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.173"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            227..2572
                     /gene="pflB"
                     /locus_tag="VCA20_002249"
     CDS             227..2572
                     /gene="pflB"
                     /locus_tag="VCA20_002249"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEP1835979.1"
                     /translation="MSTKVKTKNVAEDIFAQAWEGFKGTDWQDKASVTRFVQANYTPY
                     DGDESFLAGPTERSLHIKKIIEETKAGYEDTRFPMDVDRATSIADIPAGFIDKENELI
                     FGIQNDELFKLNFMPRGGIRMAETTLIENGYTPDPLLHEIYTKHATTVNDGIFRAYTA
                     DIRRARHSHHVSGLPDAYSRGRIIGMYARLALYGADYLMEEKVADWNAITEIDEESIR
                     LREEINLQYQALQQVVRLGDLYGVDVRKPAMNTKEAIQWTNIAFMAVCRVINGAATSL
                     GRVPIVLDIYAERDLARGTFTESEIQEFVDDFVLKLRTVKFARTKAFDEIYSGDPTFL
                     TTSMAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWSDQLPYSFRRYCMAMSH
                     KHSSIQYEGVTTMAKDGYGEMSCISCCVSPLDPESEDQRHNIQYFGARVNVLKALLSS
                     WNNGYDDVHKDYKVFDGVEPNASEIFDYDQVIANFEKALDWLTDTYVDAMNIIHYMTD
                     KYNYEAVQMAFLPTHLRANMGFGICGFANTVDSLSAIKYAQVKPIRDEDGFIYDYEVT
                     GDFPRYGEDDDRVDDIAKWLMEAFFSRLNKHKLYKNAEATVSILTITSNVAYSKQTGN
                     SPVHRGVFLNEDGSVNTTKVEFFPPGANPTSKARGGWLQNLNTLSKLNFKHANDGISL
                     TTQVSPKALGKTFDEQVNNLVTILDGYFEQGGQHVNLNVMDLNDVYDKIMAGEDVIVR
                     ISGYCVNTKYLTKEQKTELTQRVFHEVLSMDDHAAEVSGQA"
     gene            complement(2754..3544)
                     /locus_tag="VCA20_002250"
                     /pseudo
     CDS             complement(2754..3544)
                     /locus_tag="VCA20_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909160.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
     gene            complement(3626..3889)
                     /locus_tag="VCA20_002251"
                     /pseudo
     CDS             complement(3626..3889)
                     /locus_tag="VCA20_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637363.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
BASE COUNT         1222 a          915 c          783 g         1129 t
ORIGIN      
        1 catgataacg ctttctttgt caatatatca atccattata tttgaccgct gttagatatt
       61 ttagtataat gatgacttga gagaccgatg actggattat tggatttata ttccactact
      121 tggaaaaaat tccaatttat ttttcagtgt tttgtcttga agaaagtgct tacatatgat
      181 atactaaaat atgtgaaaag taacaatatc ttaaaggaga accctcatgt caacaaaagt
      241 taaaacaaaa aatgttgctg aagacatttt cgcccaagcc tgggaaggct ttaaaggtac
      301 agattggcaa gataaggcaa gtgtaacacg cttcgttcaa gccaactaca ctccatacga
      361 tggagatgaa agcttcctcg caggcccaac tgagcgttca cttcatatca aaaaaatcat
      421 cgaagaaaca aaagctggct acgaagacac tcgcttccca atggacgtag atcgtgctac
      481 ttctatcgct gatattccag caggtttcat cgataaagag aacgaactca tcttcggtat
      541 ccaaaacgat gagctcttca aactcaattt catgccacgt ggtggtattc gtatggctga
      601 aacaactctt atcgaaaacg gctatactcc agacccactc cttcatgaaa tctatacaaa
      661 acacgctaca actgtaaacg acggtatctt ccgtgcctac accgctgaca tccgtcgtgc
      721 tcgccactca caccacgttt ctggtcttcc agatgcatac tcacgtggtc gtatcatcgg
      781 tatgtacgct cgtttggcac tttacggtgc agactacttg atggaagaaa aagtagctga
      841 ctggaatgcc attactgaaa tcgatgaaga atcaatccgt cttcgtgaag aaatcaacct
      901 tcaataccaa gcattgcaac aagttgtacg cttaggtgac ttgtacggtg ttgatgttcg
      961 caaacctgcg atgaacacca aagaagctat ccaatggaca aacatcgcct tcatggcagt
     1021 atgtcgtgtt atcaacggtg cagcaacttc acttggtcgt gtaccaatcg tacttgacat
     1081 ctacgctgaa cgtgacttgg ctcgcggtac atttactgaa tctgaaatcc aagaatttgt
     1141 tgatgacttc gtattgaaac ttcgtacagt gaaattcgct cgtacaaaag ccttcgacga
     1201 aatctactct ggtgacccaa cattcttgac aacatctatg gcaggtatgg gtaacgatgg
     1261 tcgtcaccgt gttactaaga tggactaccg tttcttgaac acacttgaca acatcggtaa
     1321 ctctccagaa ccaaacttaa cagttctttg gtcagaccaa cttccgtatt cattccgtcg
     1381 ctactgtatg gcaatgagcc acaaacactc ttctatccaa tacgaaggtg tgacaacaat
     1441 ggcgaaagat ggttatggtg aaatgtcatg tatctcttgc tgtgtatctc cacttgatcc
     1501 agaaagcgaa gaccaacgcc acaacattca gtacttcggt gctcgtgtta acgttcttaa
     1561 agctcttctt tcaagctgga acaacggcta cgacgatgtt cacaaagatt acaaagtgtt
     1621 cgatggtgtt gaaccaaatg cttctgaaat ctttgactac gatcaagtta ttgccaactt
     1681 tgaaaaagcc cttgactggt tgactgacac ttatgtagat gctatgaaca tcatccacta
     1741 catgactgac aagtacaact acgaagcagt tcaaatggcc ttcttgccaa ctcaccttcg
     1801 tgctaacatg ggcttcggta tctgtggttt cgcaaacact gttgactcat tgtcagcaat
     1861 caaatatgca caagttaaac caatccgtga cgaagatgga ttcatctacg attatgaagt
     1921 aactggtgac ttcccacgtt atggtgaaga cgatgaccgt gtagatgaca tcgcgaaatg
     1981 gctcatggaa gcattcttct ctcgcttgaa caagcacaaa ttgtacaaga atgctgaagc
     2041 aacggtgtct atcttgacaa tcacttcaaa cgttgcttac tctaaacaga ctggtaactc
     2101 tccagttcac cgtggtgtat tcctcaatga agatggttct gtgaacacta ctaaggtaga
     2161 attcttccca ccaggtgcta acccaacttc taaagctcgt ggtggttggc tgcaaaactt
     2221 gaataccctt tctaaactca acttcaaaca tgccaacgac ggtatctcat tgactactca
     2281 agtttcacca aaagctcttg gtaaaacatt cgatgagcaa gtgaacaact tggtgacaat
     2341 ccttgacgga tacttcgaac aaggtggtca acacgtcaac ttgaacgtta tggacttgaa
     2401 cgacgtttat gacaagatta tggcaggtga agatgttatc gttcgtatct ctggatactg
     2461 cgtaaacact aaatacctca ctaaagagca aaaaactgaa ttgactcaac gtgtcttcca
     2521 cgaagttctt tcaatggacg accacgctgc agaagtttca ggtcaagctt aatcaagcaa
     2581 acacaaaagg acttgtacat gcgagtcctt ttgctatgtc gaaaaatctt ctgagtgcta
     2641 cggaaggata gtaaatcttc ggaaggatgc acagcagatt ttcggaagga tacagaacca
     2701 gttcataaaa aatccctttc gaaattggat tcgaaaaggg atgattattt atcctagtta
     2761 ttatagatat aggtaatttc tccgatggta tccgtcggat taaaccagcc gcggaaatta
     2821 tccacccact gttggccacg atagttggct tcgaggactt gaatttgtcc atcctcgtta
     2881 acagctgtca cataggcaac gtgccctaaa tcaccgtcat tccaggaaat aatggcaccg
     2941 acttctggca ctgttcctgt acggaaacca tcacgcgccg cattggctcc ccaagaagcc
     3001 gcatcgcccc accagtcacc tgcccaaggg gcaactttct gtactcccca tgtacattga
     3061 ccaacagggt agttaatgcc tggttcatac tcatagcctg gtagatagta gctatcagta
     3121 cttgcctcac tagcctgatt ccagatagtt tctgttaatt tcaaaatctg tccaggatag
     3181 agcggcgtct ctaaggtcat ttggttttgt gttaacaatt cgtagacgtc caatgaaaaa
     3241 gcatctgcaa tcccataaaa cgaatcccca tcttgaacaa catagtctgt ttgactcgca
     3301 actgagactt cttcattaga agcagctgga agatccaaac gttgacctgg tgtaatcagt
     3361 ccgtctattt ccatcgcatt cacttcagcc aattggtagg gatcaacccc atattgctcc
     3421 gcaatagaga acatggaatc ccttccatta ctacatgact agtggcttga acacgagata
     3481 tagttgataa cgcaactgta atccctgcaa gagctagaac agatttgcaa ctaaactttt
     3541 tcatatgaca cctcctcatc atgatttcat tttaccaaga ttttgacggg aaaagatgtc
     3601 gaaagcatta caaataaatg acgatttatc gttttatttc attctttaaa tagtttgata
     3661 taactgagaa agtcttccca ttccgaatgg tcacttgttt gtaaaaaggt gacttagcca
     3721 actgcttgtg gtaggctgtt tgtagatagc taccttcagt gtattttccc caataaatag
     3781 catgctcgta gagatgtagt acttcatcac ctagatgcaa ggactgaata tagtccacca
     3841 tagcctgctt atccaatcct tcggcgaaaa agagaacatc ctttctcgcc caatgtcatt
     3901 aagttaagca actaagcagc taaagaaact attcccgtcc gagcaattga tgacaaaacg
     3961 ggaatagttt ttgtatttag ggcgcacaaa aaggaaggtt tttaacccaa ttttttaact
     4021 attatgatat tctataagtg aaacttacg
//
LOCUS       DATUCG010000172         3918 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.174, whole genome shotgun sequence.
ACCESSION   DATUCG010000172 DATUCG010000000
VERSION     DATUCG010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3918
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.174"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(<1..204)
                     /locus_tag="VCA20_002252"
     CDS             complement(<1..204)
                     /locus_tag="VCA20_002252"
                     /inference="COORDINATES: protein motif:HMM:TIGR03726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835980.1"
                     /translation="MTKTCNHHFLVNQEKGEKHVFRKSKKYRTLCSVALGTMVTAVVA
                     WGGTVASADEVTTSVDTTIQRTEN"
     gene            483..1073
                     /locus_tag="VCA20_002253"
     CDS             483..1073
                     /locus_tag="VCA20_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HEP1835981.1"
                     /translation="MSKKEILLDFIEKNNGIVTNKDCKALGIPTIYLTRLEKEGIIFR
                     VEKGIFLTQNGDFDEYYFFQYRYPKAIFSYISALYLQQFTDEIPQYFDVTVPRGYRFN
                     TPPANLNIHSVSKEYSELGITLVKTPMGNEVRVYDLERIICDFVIHREKIDSELFVKT
                     LQSYGNYPKKNLAKLYEYATKMNTLEKVKQTLEVLV"
     gene            1070..1915
                     /locus_tag="VCA20_002254"
     CDS             1070..1915
                     /locus_tag="VCA20_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEP1835982.1"
                     /translation="MNKAKLTALCHKISKNTGLTFNSVMTYYFLEVILKKLSQSTYSN
                     HYIFKGGFLLSNVIGVESRSTVDIDFLFHQMTLSEDTVKQQLKEILADSEEGISFVIQ
                     STTAIKESDDYGGYRATILCQLENIKQIIHLDIATGDIVTPQPITYDYKAIFNEDTFP
                     IIAYTIETILAEKLQTIYSRNFLNSRSKDFYDVYILSKLKKEDIDFIQLKNACQRTFT
                     YRETELDFAKIIELLERFKSDPIQNKQWQNYSKKYSYTKGISLADVLDEMIRLITIII
                     STDSD"
     gene            complement(2001..2285)
                     /locus_tag="VCA20_002255"
                     /pseudo
     CDS             complement(2001..2285)
                     /locus_tag="VCA20_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
     gene            complement(2398..3408)
                     /locus_tag="VCA20_002256"
     CDS             complement(2398..3408)
                     /locus_tag="VCA20_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HEP1835983.1"
                     /translation="METIIEKMKKESKTGLTSGDLVDHIFKNHKIDIQDDDSVTLLNM
                     GYYHGYKRYRFVKKSNSDGILPLSSFKEIEAIYNFDMELKGLFYPIVMLVETALKNRT
                     IDSIVAESSSGIEDILEHQLACYMDYPEDHEKYGNKLDQYNETRQNVLDTIDYYSDKN
                     EVIKHYLKSDYQIPLWAYFEVITLGQFSRFLGSLTKSSREKLNQDFTIIPNRSNSLEL
                     VVNALIELRNATMHNSAIFDANFSSGVSKALKKQIKQDLGLSTFTCVFIEDYFILLIW
                     TLNILKFDTVVLNNYINQFNEIVERFDVSLNNRDILFKIISTNRKTSIQQLSKFIDEK
                     RN"
     gene            complement(3547..>3918)
                     /locus_tag="VCA20_002257"
     CDS             complement(3547..>3918)
                     /locus_tag="VCA20_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEP1835984.1"
                     /translation="GYAYGQCTWGVAARINQLGLKLKGKNGEKIPIISTMGNGQDWVR
                     TAASLGGETGTSPQAGAILSFAGGGHGTPAEYGHVAFVEKVYPDGSFLISETNYNGNP
                     NYTFRKLSGVDSSLSFAYTTK"
BASE COUNT         1275 a          741 c          649 g         1253 t
ORIGIN      
        1 attctccgtt cgttgaatgg tggtgtcaac tgaagttgta acttcatcag cagatgcaac
       61 cgttcctccc caagcaacaa cagccgtcac catggttcca agggcaacgg aacatagagt
      121 gcgatatttt ttactcttgc gaaagacgtg tttctcgcct ttttcctgat tgacaagaaa
      181 gtgatgatta catgttttgg tcataaatag tccttctttt cttttttatg tagcaacaaa
      241 ctgtcactct ttattagata cgagggcgtt aaacgagcac gacaaaaata ggagttttga
      301 cgaagaactt tccgttctag gagaaactct cttttttgta cagctcgtag cccaagttca
      361 ataccatcat aaaaaagaag gcttgatttt gttatcacaa taaattgagg taaaagaaaa
      421 tgttgctttt ataccacaaa tattgtaaaa ttgtagtgta aaagcaacaa gtgggaggcc
      481 gtatgtcaaa aaaagagatt ctactcgatt ttatagaaaa gaacaatggc attgtcacaa
      541 acaaagattg taaagcacta ggtattccaa caatatatct gacaagacta gagaaagaag
      601 gcattatctt ccgagttgaa aaaggaattt tcctaaccca gaatggagat ttcgacgaat
      661 actacttctt tcagtaccgt tatcccaagg ctattttctc ttatatctca gcgctttacc
      721 tgcaacaatt tacagatgaa attcctcaat attttgatgt gacagttcct agaggctacc
      781 gttttaatac tcctccagca aatctgaaca ttcattccgt ttctaaggaa tatagcgaac
      841 taggaattac acttgtaaaa acccctatgg gaaacgaggt aagagtgtat gatttagaac
      901 gtattatctg tgattttgtg attcatcgag aaaagattga ttccgaactc tttgtcaaga
      961 cacttcaatc ttatgggaac tatcctaaaa aaaatctagc aaaactctat gaatatgcga
     1021 caaaaatgaa tactttagaa aaagttaaac aaactctgga ggtcctagta tgaataaagc
     1081 taagctaaca gcactctgtc ataaaatatc aaagaatact ggattaacct ttaattccgt
     1141 gatgacctat tactttcttg aagtgatttt gaaaaaatta agtcagagta cctattcaaa
     1201 tcactatata ttcaaaggag gattcctact gtcaaatgtt atcggagttg aatctcgtag
     1261 cacggttgat attgattttt tgttccatca aatgacactc tcagaagata ctgtgaagca
     1321 gcaactcaag gaaattttag cagattccga agaaggtata tcttttgtga ttcaatcaac
     1381 cacagctata aaagaaagtg atgactatgg tggctatcgt gcaaccattt tatgccaact
     1441 tgagaatatt aaacaaatta tccatttgga tattgcaact ggggatattg taactccaca
     1501 gcctattaca tatgattaca aagctatttt taatgaagac acctttccaa tcattgctta
     1561 tacaattgaa acaattctag ctgaaaaact ccaaaccatc tattcacgca acttcttgaa
     1621 cagcagaagc aaagattttt acgatgttta tattctttca aaactgaaaa aagaagacat
     1681 tgacttcatt cagttgaaaa atgcctgtca gagaacattt acatatcgcg aaacagaact
     1741 cgattttgca aagattatcg aactactcga gaggttcaaa tctgacccta ttcagaataa
     1801 acaatggcaa aattattcga aaaaatatag ctatacaaag gggatttcac ttgcagatgt
     1861 gcttgatgag atgattcgtc ttattacaat tattatttct actgactctg attaaaactt
     1921 ttaactgccc aactaggtaa tcgtgacatt aaacgaaaag gatatgctaa caaatcaagt
     1981 tattgttagc atatcctttt ttatttcgtc gtataagcaa aactcaaact actatccact
     2041 ccagataatt tacggaaggt gtagttggga ttgccattgt aattggtttc tgagataagg
     2101 aatgaaccat ctgggtagac tttctccaca aaagccacat gtccgtattc tgttggtgta
     2161 ccatgtcctc ctcccgcaaa ggaaaggata gctccttctt gtggacttgt ccctgtctct
     2221 ccaccgagac ttgcggctgt tcgtacccaa tcttggccat tgcccatggt actgataata
     2281 ggcagcacaa ccgataacct ccgaagaata cttaaaaata aaaaaagagg aggactgacg
     2341 catcagaccc tcccagacgt gactagcacg ccccaattcg atacaactat gataccacta
     2401 atttcgtttt tcgtcaataa atttacttaa ttgttgaatt gatgtttttc tatttgtact
     2461 gataatctta aagagaatat cacgattatt gagtgaaaca tcaaatcgtt caacaatttc
     2521 attgaattga ttgatgtagt tattgaggac tactgtatca aattttaaaa tgttcaaagt
     2581 ccaaatcagt agaatgaaat aatcttcaat gaaaacacaa gtgaatgtcg ataatcctaa
     2641 atcttgtttt atctgttttt tgagtgcttt agatacaccg cttgaaaaat tggcgtcaaa
     2701 aatcgcactg ttgtgcatgg tagcattcct taactcaata agagcattga caactaactc
     2761 aagagagttt gacctatttg gaataatcgt gaaatcttga ttcaattttt cacgacttga
     2821 cttagttaaa cttcctaaaa atctactaaa ctgtcctaaa gtaataactt caaaataagc
     2881 ccagagagga atttgataat cactttttaa ataatgctta atgacctcat ttttatctga
     2941 gtaatagtca attgtatcca gtacattttg tctagtttca ttatactgat cgagtttgtt
     3001 accatatttt tcatggtctt caggatagtc catgtaacaa gctaattgat gttctaatat
     3061 atcttctatc ccactactag attctgcgac tattgaatcg atagtccgat tttttaatgc
     3121 tgtctctaca agcatcacta ttggataaaa tagtcctttt aactccatat caaaattgta
     3181 gattgcttca atttccttaa atgaagaaag aggcagaatc ccatctgaat tggatttttt
     3241 aacgaatcta tatcgtttat aaccatgata atatcccata tttaagagtg tgacagagtc
     3301 atcatcttga atatctattt tatgattttt aaagatatgg tcaactagat ccccagaagt
     3361 taaaccagtt ttcgactctt ttttcatttt ttcaataatt gtttccattt aaaatattca
     3421 gtaacctctc cttagtatta taccatatca ctaaacttat caaagtaact taaaggtaga
     3481 atttctaacg aaatatttac aaaacaatat gctaacaaat ccaattattg ttagcatatc
     3541 ctttttttat ttcgtcgtat aagcaaaact caagctacta tccactccag ataatttacg
     3601 gaaggtgtag ttgggattgc cattgtaatt ggtttcggag ataaggaatg agccatctgg
     3661 gtagactttt tctacgaaag ccacatgtcc gtattctgct ggtgtgccat gtcctcctcc
     3721 cgcaaatgaa agtatagctc ctgcttgcgg acttgtccct gtctctccac ctagacttgc
     3781 ggctgttcgt acccaatctt ggccattgcc catggtactg ataattggaa tcttctcacc
     3841 gtttttacct ttgagtttta gacccagttg gttgatacgg gctgcaactc cccaagtaca
     3901 ttggccgtag gcatatcc
//
LOCUS       DATUCG010000173         3884 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.175, whole genome shotgun sequence.
ACCESSION   DATUCG010000173 DATUCG010000000
VERSION     DATUCG010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3884
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.175"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            <1..421
                     /locus_tag="VCA20_002258"
     CDS             <1..421
                     /locus_tag="VCA20_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006532720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEP1835985.1"
                     /translation="QKSGATFTDSQDIAQDVLVKILEAELVLPFDKLRAWLYRSAVRV
                     YIDKYRRDKHYHEILQREFFTSEQVLVYDQDGYEELYQAVADLPAKYQHVIDLYYFQD
                     MSVKEIAHILGYSQSLVKINLYRGRKLLAKKLKRERV"
     gene            418..1401
                     /locus_tag="VCA20_002259"
     CDS             418..1401
                     /locus_tag="VCA20_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti sigma factor C-terminal domain-containing
                     protein"
                     /protein_id="HEP1835986.1"
                     /translation="MNMKTFEIATKKSKKKHLWKTVGLSILGLLLITGATLKTLQILT
                     ANNGEKIYQEHELLSQVAYPNIEYSTYYYKATSLFAGAVHADRHKDLAGVPVAFGDYE
                     YHYDWLGTYLNFSQDGPSYKGEIAYDRQTWSKLPLFYNTEKVDTDDFATVPSQELSLV
                     KDMSNQLVEVAITFDKKYSFGDLQTMLPQNLKKNWYWIGTASKKNTADFRIDQLFGFQ
                     GEALKVYEPDAELPAEAATWNPLTPMKKIAEQYNHYIGEYALVTDVKTFLDKFGKTDF
                     SKQEEINQLEFAGVILTGKAEAFAQLEGKDWIYSSSIGASIPIQPYYQLHH"
     gene            1757..2953
                     /gene="tuf"
                     /locus_tag="VCA20_002260"
     CDS             1757..2953
                     /gene="tuf"
                     /locus_tag="VCA20_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEP1835987.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDSKYEDIVMELMNTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGTVRVNDEIEIVGLQEEKSKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVISKPGSINPHTKFKGEVYILTKEEGGRHTPFFDNYRPQ
                     FYFRTTDVTGSIKLPEGTEMVMPGDNVTIDVELIHPIAVEQGTTFSIREGGRTVGSGM
                     VTEIEA"
     gene            3128..3880
                     /locus_tag="VCA20_002261"
     CDS             3128..3880
                     /locus_tag="VCA20_002261"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEP1835988.1"
                     /translation="MSRKPIIAGNWKMNKNPQEAQAFVEAIAGKLPAGDKIEAAIAAP
                     AVDLNALLWFAKDSELKVAAQNCYFEDAGAFTGETSPKVLAEMGVNYVVIGHSERRDY
                     FHETDEDINKKAHAIFRNGLTPIICCGESLETYEAGKAVEFVGAQVSAALKDLTAEQV
                     ASLVIAYEPIWAIGTGKSATKDDAQNMCKAVRDVVAADFGQEVADKVRVQYGGSVNPS
                     NVAEYMACPDVDGALVGGASLEAESFLALLNF"
BASE COUNT         1216 a          742 c          830 g         1096 t
ORIGIN      
        1 gcaaaaatca ggcgccacat ttacagacag ccaggatata gcccaggatg tactggtgaa
       61 aattttagaa gcagaacttg tccttccttt tgataaatta cgagcctggc tttatcgttc
      121 ggcagttaga gtctacatcg acaaatacag gagggacaag cactatcatg aaattctaca
      181 aagggaattt tttactagcg agcaagtatt ggtttatgac caagatggtt atgaagaact
      241 atatcaggca gtagcagacc tgccagccaa gtatcagcat gtcattgacc tttactattt
      301 tcaggatatg agtgtcaaag agattgccca tatcttagga tatagtcaga gtttggtgaa
      361 aatcaatctt taccgcggtc gaaaacttct agcaaaaaaa cttaaaagag aaagggtatg
      421 aatatgaaaa cttttgaaat tgcaacaaag aaaagtaaga aaaaacactt atggaaaaca
      481 gttggtctat ctatattagg gctcttgctg ataacgggag cgaccttgaa gaccttgcag
      541 attttgactg ccaataatgg tgaaaaaatc tatcaagaac acgaattatt gtcacaagtt
      601 gcctatccaa acattgaata ctcaacctac tattataaag cgacctctct atttgctgga
      661 gctgtccatg cagatcgcca caaagacttg gcaggggttc cagttgcctt tggtgactat
      721 gaataccact atgattggtt gggaacgtat ttgaactttt ctcaggatgg accttcttat
      781 aagggagaaa ttgcctatga tcgtcaaacc tggtcaaaac tccctctctt ctataatacg
      841 gagaaagttg acacagatga ctttgcaact gttcccagtc aagaactttc tctagtgaaa
      901 gacatgtcca atcagctagt agaagtagcc atcacttttg ataaaaaata tagttttgga
      961 gacttgcaga ccatgcttcc tcaaaattta aaaaagaatt ggtactggat cggaacagct
     1021 tctaagaaga acacagctga ttttcgcatt gatcagttgt ttggttttca aggagaggct
     1081 ctaaaggttt atgaaccaga tgctgaatta ccagcagagg cagcgacatg gaatccttta
     1141 acaccgatga aaaaaatagc agaacagtat aatcattata taggggaata cgcactcgtt
     1201 acagatgtca aaactttctt ggacaaattt ggtaaaacgg acttctctaa acaagaagag
     1261 attaaccagt tggagttcgc aggcgttatc ctaacaggaa aagcagaagc ctttgctcag
     1321 ttggaaggca aggactggat ttattcttct agtattggcg cgtccattcc tattcagcct
     1381 tattatcaac tgcatcatta agtgaaaata gtcaatatat tttcaaaacc tatagcaaga
     1441 ggcttgtcag agagtcgtag gatgactgaa tggcgaaagg tctgtcgcag tggtctcgta
     1501 tttgtggcac cttgtagcta tggtaacatc aatattctta cgggaatgag gtatttttag
     1561 ttgagtaata ggttaaaatg ggatgataaa aaaagttata agcgaagcta atagcccttg
     1621 cagaaaatgg ataaaataaa aaacgataga aaatgcttgc aattctgcta caatttgata
     1681 gaatagtaag gtaaagttag actgtattgc ctactgtcta tctataaaat atattttatt
     1741 ggaggctttt accaaaatgg caaaagaaaa atacgatcgt agtaaaccac acgttaacat
     1801 tggtacaatt ggacacgttg accacggtaa aactactttg acagcagcta tcacaactgt
     1861 attggcacgt cgcttgcctt catcagttaa ccaacctaaa gactatgcgt ctatcgatgc
     1921 tgctccagaa gagcgcgagc gcggtatcac tatcaacact gcacacgttg agtacgaaac
     1981 tgaaaaacgt cactatgctc acatcgacgc tccaggacac gcggactacg ttaaaaacat
     2041 gatcactggt gctgctcaga tggacggtgc gatccttgta gtagcttcaa ctgacggtcc
     2101 aatgccacaa actcgtgagc acatccttct ttcacgtcag gttggtgtta aacaccttat
     2161 cgtcttcatg aacaaagttg acttggttga cgatgaagaa ttgcttgagt tggttgaaat
     2221 ggaaatccgt gaccttcttt cagaatacga tttcccaggt gatgatcttc cagttatcca
     2281 aggttcagct cttaaagctc ttgaaggtga ctctaagtac gaagacatcg ttatggaatt
     2341 gatgaacact gttgatgagt acattccaga accagaacgc gacactgata aaccattgtt
     2401 gcttccagtc gaggacgtat tctcaatcac tggtcgtggt actgtagctt caggacgtat
     2461 cgaccgtggt actgttcgtg tcaacgacga aatcgaaatc gttggtcttc aagaagaaaa
     2521 atctaaagca gttgttactg gtgttgaaat gttccgtaaa caacttgacg aaggtcttgc
     2581 cggcgataac gttggtgtgc ttcttcgtgg tgtacaacgt gatgaaatcg aacgtggtca
     2641 agttatctct aaaccaggtt ctatcaaccc acacactaaa ttcaaaggtg aagtttacat
     2701 ccttactaaa gaggaaggtg gacgtcatac tccattcttc gacaactacc gtccacagtt
     2761 ctacttccgt acaactgacg ttactggttc aattaaattg ccagaaggta ctgaaatggt
     2821 aatgcctggt gataacgtta ctatcgacgt tgaattgatc cacccaatcg ccgttgaaca
     2881 aggtactact ttctctatcc gtgaaggtgg acgtactgtt ggttcaggta tggttacaga
     2941 aatcgaagct taattcgatt gagttcccag aaataacaat tatagtcaga caacttaatt
     3001 gtaactctct actttcgggt agagagtttt tctgtaagta ttcataaaat gcacagaaat
     3061 tctttaatct tagctaagtt tatgctataa tgtaccttgt acaataaata tagaagaggt
     3121 aagtgaaatg tcacgtaaac caatcattgc tggtaactgg aaaatgaaca aaaatcctca
     3181 agaagcacaa gctttcgttg aggcaattgc aggaaaattg cctgcaggcg acaaaattga
     3241 agcagctatc gcagctccag ctgtagattt gaacgctctt ttgtggttcg ctaaagattc
     3301 tgaattgaaa gttgctgctc aaaactgcta ctttgaagat gctggtgcct tcactggtga
     3361 aacatctcca aaagtattgg ctgaaatggg tgttaactat gttgttattg gtcactcaga
     3421 gcgtcgtgat tacttccacg aaactgatga agatattaat aaaaaagctc atgcaatctt
     3481 ccgtaacggt ttgactccaa tcatctgttg tggtgagtca cttgaaactt acgaagctgg
     3541 taaagcagtt gaatttgtag gtgcacaagt ttctgcagct ttgaaagact tgactgctga
     3601 acaagtagca tcacttgtta ttgcttacga gccaatctgg gcaatcggta ctggtaaatc
     3661 agctactaaa gatgatgcac aaaacatgtg taaagctgtc cgtgacgttg ttgctgctga
     3721 tttcggtcaa gaagttgcag acaaagttcg cgttcaatac ggtggttctg taaacccatc
     3781 aaacgttgct gaatacatgg catgtccaga cgttgacggt gcgcttgttg gtggtgcatc
     3841 acttgaagca gaaagcttct tggctttgtt gaacttctaa tata
//
LOCUS       DATUCG010000174         3850 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.176, whole genome shotgun sequence.
ACCESSION   DATUCG010000174 DATUCG010000000
VERSION     DATUCG010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3850
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.176"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            <1..1577
                     /locus_tag="VCA20_002262"
     CDS             <1..1577
                     /locus_tag="VCA20_002262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HEP1835989.1"
                     /translation="FNSVENEKQVYEFYIIETYENIYYVKGPQATSAIKRIRENVPYY
                     LLEIFSDDYGIVSYPSYFDKNDIKTYIEGIYNFDFPLVYRKNSNRFDCINLVVYPTKK
                     TVRFSEVILLDESYVAGDTRVKSDRDSDVVLNSIGELSPIYDIEKLDEFSDLGYPVYK
                     TYSSRGAFENIFSVHGGKGMTDFQSKCSALGEAYERYSARLFEYDEQKIICGSFTSMK
                     QRNRVLHPESLVLDVDYNNIFSEKKIFEWVEAYSLTNEKMIYVPANSVFFPYDRDNKL
                     MLHSQSTTGISAEVSTSRAILQALLEILERDSYSIVHKALLSVKEIDENTIKDEKILN
                     TINYLKVRNIRVHLSLISQFDFVYVVHCTLESETFPIFTHGSGANLNINIAIKRAIFE
                     AIQMRVSQIELKSNGDSLLKETPFIKWGEGEEEFVKPFLTKFSSEVIDVSKVIDFSTG
                     NIKTDIEFLVSKLKSNNFEVICVNLSRDDVPLSCVRVIVPGLQDIDNFNGRVTPRLAK
                     ILKEQNRQLNCIELFS"
     gene            1588..2688
                     /locus_tag="VCA20_002263"
     CDS             1588..2688
                     /locus_tag="VCA20_002263"
                     /inference="COORDINATES: protein motif:HMM:NF012703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEP1835990.1"
                     /translation="MRYFVNKYLCREVGGLRISEAFLEMSNDYADFLFVHRGQQVELE
                     LKEAEIQLLINYCVLSKFENYYNLVPNIGYTFFGIEGRCLLEDLNDGNAEKRRVGIIE
                     IPYFQGTLKHDFERDSIITLKDYSHRWPNPNQSLRSVVDKTVPSNTSICDYGFFNINF
                     SEPMLKEVESVVYRIYSSQQVFPIFIGGDHTITYPIIRGINKSGIKKLRLLYFDAHFD
                     ISPISQLHNGNVIRKIQENFDGEIEIINFGQRGPLFFEEFDSSRNIKSFHTIGELLEY
                     LKKETSIPIYISVDVDCLDPIYNPGVTYSVPNGLTLSYLENCLDSVFSVNSKILGCDI
                     VEYNSKNDFSKIGASSVNSILFNVLKLSSQIF"
     gene            2706..>3850
                     /locus_tag="VCA20_002264"
     CDS             2706..>3850
                     /locus_tag="VCA20_002264"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835991.1"
                     /translation="MFSKMKLRWPILPLDGGIEKESSDISVILDYMDIDSLSKQTNYL
                     ERMRYRPTPLFGMSYESYVQSGGLQTDFKVEDLRSYNLECYFTGRLAINPFISNDSNY
                     YTVEKNNEISRIERAKLGNLINYAKRSKYISFEEFVDLLVNTNCDIDFMDREAVAIAN
                     EIIKAGFLIPEYEEIEEVNNSFKEGILFRPTSTKMTQICHVSNRDLNALSSFFSRLLP
                     KRRISKYERLLEYFIDTYSISKIRFLELIKDKTFIRIAREVLEHSNDYQGGEDWFEYL
                     LNRIDAFRLTQNREFVDLKTKEFDFFKSSDFSFDIVTTVITENEKTLFNLDNSKPIYK
                     IFSLNADTEQLNNCYSLNYLSNDQTINFLLRNKYSNRINVNFYSSQT"
BASE COUNT         1360 a          463 c          691 g         1336 t
ORIGIN      
        1 tatttaattc agttgaaaac gagaaacaag tatatgagtt ttatataata gaaacatacg
       61 aaaatattta ttatgtaaaa ggacctcagg caacatccgc gattaagaga attcgtgaaa
      121 atgtacccta ttatttatta gaaatattca gtgatgatta tggaatcgta tcgtatcctt
      181 catattttga taaaaatgat attaaaacat atatagaggg tatttataat tttgattttc
      241 cgttagttta tcgaaaaaat agtaatagat tcgactgtat taatctagta gtgtatccaa
      301 ctaaaaaaac agttagattt tcagaggtaa ttcttctaga tgagtcatat gtcgcgggag
      361 atactcgtgt taaaagcgat cgtgattcag atgtagtatt aaattccatt ggggaactat
      421 ctcctatata tgatattgaa aagttagatg aatttagtga tttaggatat ccagtctaca
      481 aaacctactc ttccaggggt gcattcgaaa atattttttc ggttcatggt ggaaaaggta
      541 tgacagactt tcaatcgaag tgcagtgcac taggagaagc atatgagaga tattcagcaa
      601 gactatttga gtatgatgag cagaaaatta tttgtggaag ttttacttcg atgaaacaaa
      661 gaaatagggt actacatcct gagtcgctag tcttggatgt tgactacaat aatatatttt
      721 ctgagaaaaa gatatttgaa tgggttgaag catactcttt aactaacgaa aaaatgattt
      781 atgtccccgc caattctgtt ttcttccctt atgatagaga taataaatta atgctacatt
      841 cgcagtcaac tactggaatt tctgctgagg tatctacttc aagagccatt ttacaagctt
      901 tattagaaat tcttgaaaga gattcctatt ctattgtgca taaagcatta ctgtccgtta
      961 aagaaattga tgaaaataca attaaagatg aaaaaatact aaatacgatc aattatttaa
     1021 aagtcagaaa tattcgggtt catttatcat taattagtca gtttgatttt gtatacgtag
     1081 tacactgtac attggaatca gaaacttttc cgatatttac tcatggttct ggagcgaatt
     1141 taaatattaa tattgccata aaaagagcta tattcgaagc aatacaaatg cgagtttctc
     1201 agattgaact gaaaagtaat ggtgatagtc ttttaaaaga gactccattt ataaaatggg
     1261 gagaagggga agaagagttc gttaaacctt ttttgactaa attttcttct gaggtaatag
     1321 atgttagtaa ggtcatagat ttctcaactg gaaatattaa gaccgatata gaatttttag
     1381 ttagtaaatt aaaaagtaat aattttgaag ttatatgtgt aaatttatct agagatgatg
     1441 ttccgttgtc ttgcgtaagg gtcattgtac caggtttaca agatatcgat aattttaatg
     1501 gtagagtgac gccaaggtta gccaaaatat taaaagaaca aaataggcaa ttaaattgca
     1561 ttgaattatt tagttaggat tattcaaatg aggtactttg ttaataaata tttatgtaga
     1621 gaggttggag ggctccgtat ttcagaagcc tttttagaaa tgtcaaacga ttatgctgat
     1681 tttttatttg ttcacagagg tcagcaggtt gagttagaat taaaagaagc tgaaattcag
     1741 ttattaatta actattgtgt tttgagtaaa tttgagaatt attataactt agttccaaat
     1801 ataggatata ctttttttgg tattgaagga cggtgtttac tagaagattt gaatgatgga
     1861 aacgctgaaa agagaagagt tgggataatt gaaatacctt attttcaggg gacgttaaaa
     1921 catgattttg agagagattc gatcataact ttaaaggatt actctcacag gtggccaaac
     1981 ccaaatcagt ctttaaggag tgtagtagat aaaacagtgc catccaacac atcaatttgt
     2041 gattatggct tctttaatat taatttttcc gagccgatgt taaaggaagt tgaatcagtt
     2101 gtatatagaa tatattcgtc tcaacaagtt tttcctattt ttattggagg agatcatacg
     2161 ataacgtacc ctattatcag agggataaac aaatcgggta ttaaaaaatt acgtttactt
     2221 tattttgatg ctcattttga tatttctccg atttcccaat tacataacgg aaatgtgata
     2281 aggaaaatcc aagagaattt tgacggtgag attgaaatca ttaattttgg gcaacgagga
     2341 ccattatttt ttgaagaatt tgattcatca agaaatataa agagttttca tactataggg
     2401 gaactattag agtatttgaa aaaagaaaca agtattccta tttatatatc tgtggatgtt
     2461 gattgtttag atcctattta taatcctgga gtcacttatt ctgttccgaa tggattgact
     2521 ttatcatact tagaaaactg tttagattct gttttttctg taaattcaaa aatattagga
     2581 tgtgatattg ttgagtataa ttccaaaaac gacttttcta aaataggtgc aagttctgtt
     2641 aattctatcc tgttcaatgt attgaagtta agttcgcaaa ttttttgaga ggtttagata
     2701 tacttatgtt ttctaaaatg aaattgagat ggccaatact tccgttagac ggcggtattg
     2761 aaaaagagag ttcagatatt tctgtaattc ttgactatat ggatattgat agtttatcta
     2821 aacaaacaaa ttatctagag cgtatgaggt atagacctac tcctctattt gggatgagct
     2881 atgaaagcta cgttcagagt ggggggcttc agacagattt taaagtagaa gatttacgtt
     2941 cctataactt agaatgttat tttacaggaa gattagctat caatccattt atttcaaatg
     3001 atagcaacta ttacacagtt gaaaaaaata atgaaatttc tagaatagaa cgtgcgaaac
     3061 tagggaatct aattaactat gcaaaacgta gtaaatacat tagctttgaa gaatttgttg
     3121 atttattagt aaatacaaat tgtgatattg attttatgga tagagaggca gttgctattg
     3181 ctaatgagat aattaaagct ggttttttaa tacccgaata tgaagaaata gaagaagtaa
     3241 ataattcgtt taaagaaggg attttatttc gtcctaccag tactaaaatg acacagatat
     3301 gtcatgtttc taatagagac ttaaatgctt tatcttcttt ttttagtcgt ttattaccta
     3361 aacgaaggat atcgaaatat gaacggttac tagaatattt tattgatact tactcaatta
     3421 gtaaaattag atttttagaa ttaattaagg ataagacatt tattagaatt gccagagaag
     3481 tcttggagca tagtaacgat tatcaaggtg gagaggattg gtttgaatat ttacttaatc
     3541 gaatagatgc gtttcgtctt acacaaaata gagaatttgt tgatttgaaa actaaagaat
     3601 ttgatttttt taaaagtagt gacttttcat ttgatatagt gactactgtg attactgaaa
     3661 atgaaaaaac tttattcaat ttagataatt ccaaaccaat atataaaata ttttctctta
     3721 atgcagatac agagcaactg aataattgct attcgcttaa ctatttatca aacgatcaaa
     3781 caattaactt tttacttagg aataaataca gtaatagaat aaatgttaat ttttattcaa
     3841 gtcagacatt
//
LOCUS       DATUCG010000175         3795 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.177, whole genome shotgun sequence.
ACCESSION   DATUCG010000175 DATUCG010000000
VERSION     DATUCG010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.177"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(272..913)
                     /locus_tag="VCA20_002265"
     CDS             complement(272..913)
                     /locus_tag="VCA20_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1835992.1"
                     /translation="MNNSIIKIENLSKKFKNCDLFDNLHFECSEGEIVGITGRNGSGK
                     SVLFKLIAGLMQPNSGTIKVYGEDIVGKIPSGLGALIEEPGFIPNYSGFENLEFLSTI
                     QNIIGKEEILATLHRVGLFEDKDKKVRNYSLGMKKKLGIAQAIMEEPRILLLDEPTNA
                     LDSQSVQRVYDLLKELSKEKGVTVLLASHNDSDIRHLCDKVYLLENKQCKLLK"
     gene            complement(906..1637)
                     /locus_tag="VCA20_002266"
     CDS             complement(906..1637)
                     /locus_tag="VCA20_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835993.1"
                     /translation="MRESSIIKNSLFWEWRCLTTSSQFYIYLILSGMYYLLLALLNFF
                     EVGFGEYLLKGPGLDGIETISLLSYLLFQAQNIFLLQQLFSGLSTTNFNLTILRIGSI
                     KDWFLVKLVSLIFLSLLLGLFQTVPLFFIGNMDYLFFLHFFVVNSTNFILFSSLLFLL
                     FHFDFSDAVSFCITYSLLVFIIFSGEILGENTNYFVTILARVAEYEKNSNLSLFIAIF
                     FEIILSAAILSFIKIRYSLGGKIHE"
     gene            complement(2401..3165)
                     /locus_tag="VCA20_002267"
     CDS             complement(2401..3165)
                     /locus_tag="VCA20_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835994.1"
                     /translation="MKFLLMNVRRTVFSKKMLLSFLLAFSCILIGNMVAFNGIYKLEG
                     LSLFFAGSTIRGFSPISLVAAVISAIPIADRVIEDSKNHFLRLQLQRTSRIKYIWTLL
                     VTAGISGFLSLFLPYFLLLVANLCLTPYKEIYIGDYQGVFKSIFDSNQLVYSILITIW
                     YGIFGSVFAVFGLASSLAFRQKIVGVFFPCLYMILGGLFFALLDLSFLEPVGIISWGY
                     QFQLNFLLVFLHLLCIFTICLGMILYHFQFRVEDSI"
     gene            complement(3250..3660)
                     /locus_tag="VCA20_002268"
     CDS             complement(3250..3660)
                     /locus_tag="VCA20_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1835995.1"
                     /translation="MKTRLSIKKPIIVAIALLGLVSPTVFSRGVLDYSMVLPRIGSTT
                     TGSIVKVTHSRGVNRNDSTGGGKTLNTSIRNYHSGSDITPTYSLPSGARVLMSYHGSG
                     SHYVNETTTLGLATQFGNTVRVQTTGSWSPDEEQ"
BASE COUNT         1444 a          700 c          558 g         1093 t
ORIGIN      
        1 tcttttgaga ttgccatgat atgttctcct ttgttttgag cctaatccga gtgacaggtt
       61 ggcgacctgt aaccaagaaa aagctagatt tgtccttttt ggacctcatt tattctatca
      121 aggaataact cttttgtcaa ctgatattat cttccaataa accgagtaga ctttctctat
      181 tctttttcaa attcaccttt acaaattagc cgtaccgtca aaatagtcac gaattttgta
      241 caaaactgaa tgcacaattc ataaattatt tctatttcaa taacttacat tgcttatttt
      301 cgagaagata aactttatca catagatgtc taatatcact atcattatga ctggccaata
      361 aaaccgtaac acccttttct tttgaaagtt cttttagcaa gtcatacacc ctctgaacgc
      421 tctgactatc caaagcattt gtcggctcat ccaatagaag gattctaggc tcttccataa
      481 tagcctgtgc aattcctaat tttttcttca taccgagaga gtaattccta acctttttat
      541 ccttatcttc aaagagtcct acacgatgta aggttgcaag tatttcttcc ttaccaatga
      601 tattttggat agttgataaa aattctaaat tttcaaatcc actataattg ggaataaagc
      661 ctggttcttc aattaatgct cctaaaccag atggaatctt tccgacaata tcttctccat
      721 acaccttgat agtcccagaa ttcggttgca taaggcccgc tatcaatttg aacagaacac
      781 tcttccccga accatttcta ccagttatcc ctactatttc cccttccgaa cactcaaagt
      841 gtaaattatc aaataaatca caattcttaa attttttgct aaggttttca atttttataa
      901 tactattatt catgtatttt acctcccaaa gaatatctga tctttataaa agaaagtatc
      961 gccgcactta gtataatctc aaagaatata gcgatgaaaa gtgacaaatt tgaatttttc
     1021 tcatactcag ctacccgtgc caatatcgta acgaaataat ttgtattttc tcctaaaatt
     1081 tctcctgaaa aaatgataaa aacgagtaaa gaataagtaa tgcaaaacga gacagcatcc
     1141 gaaaaatcaa aatggaacaa taaaaagagc aatgaactga acaaaataaa attagtgcta
     1201 ttaactacga aaaaatgcaa gaaaaacaaa tagtccatat tacctataaa aaacagagga
     1261 actgtttgaa acaaccccaa taaaagtgat agaaatatca aggacacaag cttgacaaga
     1321 aaccaatctt ttatactccc aattcttagt atcgttaagt tgaagtttgt tgttgataaa
     1381 ccagaaaata gctgttgcaa caaaaaaata ttctgtgctt gaaacaatag gtaagaaagt
     1441 agactaatgg tttctatgcc atccaaccca ggacctttta gaagatactc accgaagccg
     1501 acttcaaaaa aattcaatag cgctaacaat aagtaataca tccctgataa aattagataa
     1561 atataaaact gagagctagt agtcaaacaa cgccattccc agaataaaga attttttata
     1621 atggaactct ccctcattcc tattcctccc actctgtatt tcttatttta tataggttta
     1681 acaaaatgaa aaaaagcaag agaattagcc aatacaataa ctccttgtgg taacttactg
     1741 attttaaaat atagtgatgg ataggaaaga cttgactaat ggttgcattg atcggataaa
     1801 accagaaaac agattgtatt aacaaaaatg agaaaagatt agccatgact gtacttcctt
     1861 tttgaaaaag aagaaaatta aacaaggcta tttccgagag gaaaataaga taacataaaa
     1921 aactaaacaa gggcaaaagt atactttgaa aattagaaac gtaagatatt tcataaactt
     1981 ctgatagaag tggttgatgt atagaagaaa ttgaaaaaga aaacgaattt accaacccca
     2041 tcaaaaatac cagacacatc agaagaaata caaataagaa tggaagtagc aagctataat
     2101 ttaataaaaa gtaatactta tctttgatgg ataaaaaacg cagtgtgaca aatgtatctc
     2161 ttgtaaaaac ttctatacaa aaaaataaaa tagcagtgta gataatcggt atcaggtaga
     2221 cacctgttgt tatatcttgg catagaatca gtagccaggt aaaaacattt ggaacaagtt
     2281 gataatgacc taaataatat tgataggtgg tatttgaaaa aatcaaactc ccaagtaaga
     2341 tgataaaaaa taatctgaag atagtagatt ttttatgaaa aaatagaatt ctacaaaatc
     2401 tcatatacta tcctccactc ggaactgaaa atgatataaa atcattccaa gacagatggt
     2461 aaaaatacac agtagatgca aaaatacaag tagaaaattt aattgaaact ggtaacccca
     2521 agaaataatc ccaacaggct ctaaaaaaga caaatctagc aaggcaaaaa acaacccgcc
     2581 caaaatcata tagaggcaag ggaaaaatac cccaacaatt ttttgacgaa aagcaagaga
     2641 actagctagg ccaaatactg caaaaacgct accaaaaatc ccataccata tcgttattaa
     2701 gatagaataa actaactgat tactatcaaa aatagactta aaaacacctt gatagtcacc
     2761 aatatatatt tccttatatg gcgtcagaca tagatttgca accaaaagca ggaaataggg
     2821 taaaaaaagg gacaaaaatc ctgaaattcc tgccgtcaca agaagagtcc aaatatattt
     2881 tatacgggat gttctttgta attgcaggcg cagaaagtga ttcttactat cctcaataac
     2941 ccgatcagct atcggaatcg cactgataac tgctgccaca aggcttatcg gcgaaaaacc
     3001 cctgatagtc gatcctgcaa aaaacaggga caatccttca agtttgtata tgccattaaa
     3061 agcaaccata ttccctatca aaatgcagga aaatgccaag agaaaagaca aaagcatctt
     3121 tttagagaaa acagttcttc taacattcat tagtaaaaat ttcattttta cctcctagta
     3181 aaaagagcta ggtgaataaa ttccactagc tcaatagtct caataaacaa cttcctagct
     3241 cattactaat cactgttctt catctggact ccaactgcca gtcgtttgta ctctaactgt
     3301 atttccgaat tgagtagcta atcccaaagt agtcgtttca ttgacataat gactaccact
     3361 tccatgataa gacatgagaa ccctagcacc agatggaaga gaataggtcg gagttatatc
     3421 actaccgctg tggtaatttc gtattgaggt atttaaagtc tttccaccac ctgtagaatc
     3481 attgcgattc actcctcgcg aatgagtcac cttgactatg ctaccggttg ttgtagatcc
     3541 tatacgtggt aagaccatgg aataatctag tactcctctt gaaaaaaccg tcggagaaac
     3601 taatcccaat aatgctattg ctacgataat tggctttttt atagataatc tcgttttcat
     3661 gtgaatcttc tcctttcatg aaaaccttat aaggaagttg tttatattga tatgtaaatt
     3721 atatcaagta tggtaaaaaa tatcaaggtt ataattaaat aaacttagga aatatgacag
     3781 gagaattaaa caact
//
LOCUS       DATUCG010000176         3777 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.178, whole genome shotgun sequence.
ACCESSION   DATUCG010000176 DATUCG010000000
VERSION     DATUCG010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.178"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(<1..486)
                     /locus_tag="VCA20_002269"
     CDS             complement(<1..486)
                     /locus_tag="VCA20_002269"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010925605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HEP1835996.1"
                     /translation="MIQLQRTQPTLQPFTKWTGGKRQLLPVIKSLMPDNYNSYFEPFI
                     GGGAVFFELIPKKAIINDFNSELINCYRQIKDNPQKLIELLTKHQENNSKEYYLELRA
                     VDRDNRINKMTDVERAARIMYMLRVNFNGLYRVNSKNQFNVPYGRYKNPKIVDSELIL
                     SI"
     gene            complement(576..3401)
                     /gene="uvrA"
                     /locus_tag="VCA20_002270"
     CDS             complement(576..3401)
                     /gene="uvrA"
                     /locus_tag="VCA20_002270"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003037804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEP1835997.1"
                     /translation="MQENIVIHGARAHNLKNIDVTIPREKLVVVTGLSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGNMDKPDVDSIEGLSPAISIDQKTTSRNPRSTVGTA
                     TEINDYLRLLYARVGTPYCINGHGAIAASSVEQIVDEVLELPERQRLQILAPIVRKKK
                     GQHKTVFEKVQKDGYVRVRVNGDVYDVSEVPELSKSKAHNIEVVVDRIVIKEGVRSRL
                     FDSIEAALRIADGYVIIDTMDDKELLFSEYYACPVCGFTVPELEPRLFSFNAPFGSCS
                     DCDGLGMKLEVDTDLIVPDASKTLRDGALAPWNPISSNYYPQMLEQAMNHFGVDMDKP
                     FEELTEEEKNLIFNGSDGKEFHFHYENEFGGVRDIDIPFEGLITNINRRYRETKSDYT
                     RTVMKTYMNELTCGTCHGYRLNDQALSVKVGGEQGLHIGQLSDLSVADHLEVIENLTL
                     SENEATIATPIVKEIKDRLSFLNNVGLNYLTLSRAAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIASLKKMRDLGNTLIVVEHDEDTMREADWLIDIGPGA
                     GVFGGEIVASGTPAQVAKNKKSITGQYLSGKREIPVPLERRVGNGRFLEVTGAKENNL
                     QDVTVRFPLGKFVAVTGVSGSGKSTLVNSILKKAIAQKLNRNSDKPGKFKTISGIEHL
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVFVPCEVCHGHRYNSETLEVHYKEKNIAQVLDMTVNDAVE
                     FFKHIPKIERKLRTIQDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSLYIL
                     DEPTTGLHTEDIAQLLKVLARFVDDGNTVLVIEHNLDVIKTADHIIDMGPEGGVGGGT
                     VVATGTPEEVAENPASFTGQYLKMKLK"
BASE COUNT         1064 a          927 c          778 g         1008 t
ORIGIN      
        1 aatagataaa attaactcac tatcaacaat cttaggattt ttatagcgac cataaggtac
       61 attgaactga tttttagaat taacacgata caagccatta aaattcactc gcaacatata
      121 cataatacgt gcagcacgct caacatcagt cattttatta atcctattat ctctatcgac
      181 agcacgtaat tctaagtaat actctttaga gttattttct tgatgtttag ttagtaactc
      241 aatgagtttt tggggattat cttttatttg acgataacag ttgataagct cactattgaa
      301 atcattgata attgcttttt tcggaatgag ttcgaaaaat accgccccac caccaatgaa
      361 tggttcaaag taactattat aattgtctgg cataagagat tttataaccg gaagcagttg
      421 tcgcttccca ccagtccatt ttgtaaaagg ttgtaaagtt ggttgagttc gttgtaattg
      481 tatcatcgtt tatattatac catatctcta aatatatttg attaattgaa atggaatagt
      541 ctccaacatg caaaaaaagc cgcagaggcg actttttatt tcaacttcat tttcagatac
      601 tggcctgtaa agctggctgg gttctccgcc acttcttctg gtgttcctgt cgcaacaacg
      661 gtaccaccgc cgacaccgcc ttctggcccc atgtcgataa tgtggtcggc tgtcttgata
      721 acatccagat tgtgctcgat gaccagcacg gtattgccat catcaacaaa gcgagccaag
      781 accttgagca actgggcaat gtcttcggtg tgcagacctg tcgtcggctc atccaagata
      841 tacaaggatt taccagttga ccgcttgtgg agttcagaag ccaacttcat ccgctgggct
      901 tccccacctg atagggtcgt tgctggctga cctagagtaa cgtagcccaa gcccacatct
      961 tgaatggtgc gaagtttccg ctcaatcttt ggaatatgtt tgaagaactc caccgcatca
     1021 ttgacggtca tatcaaggac ctgagcgata tttttctcct tgtagtgtac ttctaaggtt
     1081 tcagaattgt aacggtgtcc atgacagacc tcacatggca caaagacatc tggcaggaag
     1141 tgcatttcaa tcttgatgat accatcacca gaacaagcct cacaacgacc acctttgacg
     1201 ttaaaggaga aacgtccttt cttgtagcca cggattttgg cttcattagt ctgggcaaag
     1261 aggtcacgaa tatcgtcaaa gacacctgta taggtggctg gatttgaacg tggtgtccgt
     1321 ccaatcggac tttggtcaat atcaatcaag cgatccaagt gttcaattcc tgaaatggtt
     1381 ttgaacttgc ccggcttgtc tgaattacga ttaagtttct gggcaatggc ttttttcaga
     1441 atggaattga ccagagttga cttaccagag ccagacaccc ctgtcaccgc cacaaatttt
     1501 ccgagtggga aacggaccgt cacatcctgc aggttgtttt ctttggcacc tgttacctcc
     1561 aggaaacgac cattgccaac acggcgttct agagggacag gaatttcacg tttgccagac
     1621 agatactgac ctgtaatgga ttttttattt ttagctactt gggcaggcgt tccagaggcc
     1681 acgatttctc cgccaaagac acccgcacct ggaccaatgt caatcaacca gtccgcctca
     1741 cgcatggtgt cctcatcatg ctcaacaaca atcaaggtat tacccaaatc acgcattttc
     1801 ttaagactgg caatgagacg gtcattgtcc cgctggtgca gaccaatcga cggctcatct
     1861 agaatataga ggacaccaga caggttggac ccaatctggg tcgccaaacg aatccgctga
     1921 ctctcaccac cagaaagggt acctgctgct cgtgaaagtg tcagatagtt gagaccaacg
     1981 ttattaagga aggacagacg atctttgatt tccttgacaa tgggcgtcgc aatggtggct
     2041 tcattttcag acaaggtcag attttcaatg acttccagat gatccgctac ggacaagtca
     2101 gacaactgcc cgatatgaag tccctgctcg ccaccaactt tgacagacaa ggcttggtca
     2161 ttgaggcgat agccgtggca ggttccacag gtcaattcat tcatgtaagt cttcatgact
     2221 gtccgtgtgt aatcactctt agtttcacgg tagcggcgat taatgttggt aatcaagccc
     2281 tcgaatggaa tgtcaatatc acgcacccca ccgaactcat tttcataatg gaaatggaac
     2341 tctttcccgt cagaaccgtt gaaaatcagg ttcttctcct cttccgtcaa ttcctcaaaa
     2401 ggcttgtcca tgtccacacc aaaatggttc atggcctgct ccaacatctg agggtagtag
     2461 ttagaagaaa taggattcca cggagccaaa gccccgtcac gcaaggtctt gctggcatct
     2521 gggacaatca aatccgtatc cacttccagc ttcatgccca aaccgtcaca gtcgctacaa
     2581 gatccaaaag gagcattgaa agagaagagg cgaggctcca actcgggcac tgtaaagcca
     2641 caaactggac aggcatagta ctcagagaaa agcagctcct tgtcatccat ggtatcaatg
     2701 atgacataac cgtccgcaat ccgcagggcc gcttctatag aatcgaagag ccgagagcga
     2761 acaccttctt taatcacaat tcgatcgacc acaacctcaa tgttatgagc cttgctctta
     2821 gacaactcgg gcacttcaga cacatcatag acatcgccat tgacacggac ccgaacatag
     2881 ccgtccttct gcactttctc aaaaacggtc ttgtgctgac cctttttctt ccgcacaatc
     2941 ggtgctagaa tctgcaagcg ttgacgctct ggcaattcca aaacttcatc tacgatttgc
     3001 tccaccgagg aagctgcgat ggctccatgg ccattgatac agtatggtgt ccccactctt
     3061 gcataaagca ggcgaagata gtcattgatt tctgttgccg ttccaaccgt tgaacgcggg
     3121 ttccgcgagg tcgttttttg gtcaatggaa atagcagggc tgagaccctc tatagaatcc
     3181 acatccggct tgtccatatt gcccaagaac tgacgagcat aggcagacaa agattccaca
     3241 taacgacgtt gaccttcggc atacaaggta tcaaaagcca aactcgactt accagaaccc
     3301 gacaacccag tcaccaccac cagcttctca cgcggaatgg tcacatcaat atttttcaaa
     3361 ttatgggcac gcgccccatg aatcacaata ttttcttgca taatttaaga tacaaagggc
     3421 gtaaaacgca cagtcaaaat aggcggtaag tccgaaatct gtgatttcga agacgcaccc
     3481 ccgtcaagac aactagtgct ttcacaggtc gaaaaccgaa gaaagcacta gttgtctacg
     3541 actcagaagc taattcccca tactcataca tgaagaaacc agcaattttt gacaccgcat
     3601 ttagcctatg ttcaattcct ttcttaagag gctataagct atggtatacg gaacaaggaa
     3661 aagtccgtac aacttattcg cttaaatgtc tatacccgtg gtgctagttc atttcaaaat
     3721 agcgtaagtt atgagctaga ataatttgtt ccattcgtaa ctgtagtccg gctaggc
//
LOCUS       DATUCG010000177         3674 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.180, whole genome shotgun sequence.
ACCESSION   DATUCG010000177 DATUCG010000000
VERSION     DATUCG010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3674
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.180"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            98..319
                     /locus_tag="VCA20_002271"
     CDS             98..319
                     /locus_tag="VCA20_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEP1835998.1"
                     /translation="MNVILRKTGNSTVITIPNKIKETLGAEIGEEIEFVTSGNNVIIR
                     KAEPKFDFDKELEKAMNQYDELLKELVDR"
     gene            316..723
                     /locus_tag="VCA20_002272"
     CDS             316..723
                     /locus_tag="VCA20_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEP1835999.1"
                     /translation="MRYLTAEDIIKINVMVIGKYSPNEPVGIADPNSLQMIVEQPKQE
                     IFGKVLYPDIYSKAAIIWINLIKKHPFYNANKRTAVLALHIFLAMNGYQSNISLQDGL
                     EKTVEIATFQGDFEDLKKTIIHFLKQDGNITKL"
     gene            complement(799..1862)
                     /locus_tag="VCA20_002273"
                     /pseudo
     CDS             complement(799..1862)
                     /locus_tag="VCA20_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502723.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
     gene            complement(1896..2218)
                     /locus_tag="VCA20_002274"
                     /pseudo
     CDS             complement(1896..2218)
                     /locus_tag="VCA20_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480469.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            2220..2555
                     /locus_tag="VCA20_002275"
                     /pseudo
     CDS             2220..2555
                     /locus_tag="VCA20_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288973.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2657..3406
                     /locus_tag="VCA20_002276"
     CDS             2657..3406
                     /locus_tag="VCA20_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEP1836000.1"
                     /translation="MQKIELLDGERIDQLFLTDVQIIQNREVFSYSIDSVLLSRFPKM
                     PAQKGMIVDLCSGNGAVGLFASTRTKAQIIQVELQERLAEMNRRSIALNGLEEQVSVI
                     NDDLANLPQYDLRSKVDLMLCNPPYFKVDKESNLNESEHYLLARHEIATNLDSICQVA
                     QQVLKSNGRLAMVHRPDRFLDILDTLRTYKLAPKRIQFVYPKASKEANMLLIEAIKDG
                     SVDGLHILPPLVVHEENGDYTSAIREIYYGK"
     gene            3396..3668
                     /locus_tag="VCA20_002277"
     CDS             3396..3668
                     /locus_tag="VCA20_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEP1836001.1"
                     /translation="MENKAYLYVLECADGSLYTGYTTDVEKRLATHNRGKGAKYTRAR
                     LPVSLLYQEAFASKQEAMSAEALFKKRSRQSKLDYIAEMTKKPRTK"
BASE COUNT         1136 a          767 c          712 g         1059 t
ORIGIN      
        1 agggcttctc actttaatta taaatcaaaa taaagagcat aacacctttt cattcagtgt
       61 tgtgccttga gtgaaacgaa aggaataaat tataaccatg aatgttatct tgagaaaaac
      121 aggaaatagt actgtcatta caattcccaa taaaattaaa gaaactcttg gagcagaaat
      181 tggagaagaa attgaattcg tcacttcagg aaataacgtc atcattcgta aagctgagcc
      241 aaaatttgat tttgataagg aactagaaaa agccatgaac caatacgatg agcttttgaa
      301 agaattggtg gatagatgag atacctaact gctgaagata ttatcaaaat caatgtcatg
      361 gttattggta aatattctcc taatgaacct gttggcattg ctgatccaaa cagtcttcaa
      421 atgattgttg aacagcctaa acaagagatt ttcggaaaag ttctctatcc agatatctac
      481 agtaaagctg ctattatctg gataaatttg attaaaaaac atccttttta taatgctaat
      541 aaacgaactg ctgttcttgc cttgcatata tttctagcaa tgaatggtta ccaatccaat
      601 atttccttac aggatggact tgaaaagaca gttgaaattg ctacctttca aggggatttt
      661 gaagatttaa agaaaacaat tattcacttc ctcaagcaag atggaaacat aacaaaatta
      721 taattaaaaa gtggttatca acctttcgtt gaagaccact ttttatgttc tactgtatcc
      781 tacttcataa aggataaact atctgtcaac caaggtatag tactgatttc tatcattttt
      841 gtttgcgccg taccaattaa ggatgcccaa ttcttgtaag tttttcatag tttttacaat
      901 aaatttacgg cttctaccag taatttcaat cgcctttgct gttgtcattt tctcgccaga
      961 attgtacata tagtggataa tagcctgctc gtctgcgttt agattaccaa aatcagaaaa
     1021 ctatttttcc aaactgtctc gtgttcgcag atgtcgacta acaatgttat tttcaaggat
     1081 tagaactacc ttattacctg gctctgaata tttaggttca tgaagaaaag ctgattccat
     1141 ttcagaatag atacgcttaa caccctcgtt catttcacga acccatccaa attcagtcag
     1201 ggttctcgca atccgaggat ttctcgaaaa acgttcatgc ttgatattgt caacagtaac
     1261 gatatttggc aatttccccg ggctatgaat ctccagtcta tcatcaaaca tgagtacacg
     1321 aacgtgatct ccatagacag agtaatcacg atgagtcaca gcattaacaa cgccttcaaa
     1381 ccaagcaaat tcaggatact caggtaaaat ctggaattgc ccattatcgt ctaagtactg
     1441 aaattcacgt aactgagtgc ggataaaatc tcgcactttg ataatcaagg taggtaggca
     1501 tcatcaaaag tcacttcttt gatgacatta aaactactac ctatccccat atcagtcccg
     1561 tcaaaccgtt gaaaacgaac acgagcttgc ggaaagaaag ctgacggata ttttccaaag
     1621 aggagaatcg ctgacgtggt tagtttgcca ttcacgagaa aacgccttgc tttaaggatt
     1681 tcttctgtcg aacggtttaa aatatcaaac cgatttttaa aatcctgaac caaattgtca
     1741 tcaatgtctt ccaaggttgc atcaggcacg atttcatctt caaagaaacg ttgtccctta
     1801 tcatagctaa gctgagtacg ttgctcatag cttaatttaa ccgattcatc gccctgacgc
     1861 agataaacct catcattagg cgcagcaatc actcggttag atgacaattc aactgagata
     1921 accaaaatca gatcatcttc ccccttatga ttaaccacag ggatttcctc aaagaacaaa
     1981 tccagaggag tctcccgcat atcacggtca atctttttga aatcgtctat gggataagat
     2041 ctaccatcct tgaaacctgt gatgatattg tctgcttctc atcctcaatc ccaataacca
     2101 gctgaccgcc atcagcatta gcaaaagcaa tcaggtgctt gagcaattca gatggtttct
     2161 tacgtgctga tttgcggtct aaatactggt tttctggtga gaattggtag taagtcaacg
     2221 tttttcacga ttcttgggat tcaaccaaag tctgtatcct tcattctgac caaggttttc
     2281 aatataccaa tcaacagtat ctccgaaaat tagaccaata tggtgtgacc atatctcatt
     2341 ctggcaaggg aaattgttat gataatgcag catgtgagaa tttcttttcc cacttgaaga
     2401 gtgaatcctt gagactattt ccgcctgagg atagggaagc cctcatccaa caaatcaggg
     2461 aatacatcga ctggtgtaat tggcacagac cacaagaaaa attaaaaggt atgactcccc
     2521 tagaatttag ggaagcatac cttctaaaaa actaactttt ttaactgtcc attttatctg
     2581 gtgcttgaca gggtattcgc tctcttttca ttcaaaataa acagtgattt tcattcaaag
     2641 atggtataat atacctatgc aaaaaattga gttattagac ggggagcgga ttgaccagct
     2701 cttcttgaca gatgttcaga ttattcaaaa tcgggaggta tttagctact cgattgacag
     2761 cgttcttctt tcgcggtttc caaaaatgcc tgcacagaag gggatgattg ttgacctttg
     2821 tagtggaaat ggggcggtgg gcctcttcgc ctccacacgt accaaggccc agattattca
     2881 ggtcgagttg caggaacgct tggcggaaat gaaccgccgt tccattgccc tcaacggtct
     2941 ggaggagcag gtttcggtta tcaatgacga cttagcgaat ctgccccaat atgacctacg
     3001 gtccaaggtt gatttgatgc tctgcaatcc tccctacttc aaggtagata aagagtcaaa
     3061 tctcaatgaa agcgagcatt atcttctagc cagacacgag attgcgacca acctagacag
     3121 tatttgccag gtggcccaac aggtgctcaa gtccaatggt cgcttggcca tggttcacag
     3181 accggaccga tttttagata ttttagacac actaaggacc tacaagctag ctcccaaacg
     3241 catccagttt gtctatccca aggccagcaa ggaggccaat atgttgctga ttgaggctat
     3301 caaggacggc tcggtggacg gtctgcacat cctgccacct ctggttgtcc atgaggaaaa
     3361 cggcgactat acttctgcca ttcgtgagat ttattatgga aaataaggcc tacttgtatg
     3421 ttttggaatg tgccgacgga agtctttata ctggctatac gactgatgtg gagaagcggc
     3481 ttgctaccca taatcgtggg aaaggggcca agtacacccg cgcacgcttg cctgtcagcc
     3541 tgctctatca ggaagccttt gctagtaagc aggaagctat gtctgccgag gctttgttta
     3601 agaaacgcag tcggcagagt aagttagact atatcgcaga aatgacaaaa aaacctcgta
     3661 caaaatagta cgag
//
LOCUS       DATUCG010000178         3645 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.181, whole genome shotgun sequence.
ACCESSION   DATUCG010000178 DATUCG010000000
VERSION     DATUCG010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3645
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.181"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(156..794)
                     /locus_tag="VCA20_002278"
     CDS             complement(156..794)
                     /locus_tag="VCA20_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEP1836002.1"
                     /translation="MIIHKSVNPVAFQNTYYLENDTHLIIIDPGSDWTKIQVTIEKIG
                     KPISAILLTHTHYDHIISLDILRETYNFPPVYVAESEARWLYIPEMNLSGLPRHDDME
                     NVVCRPAEKIFQYQEDYQFDGFHFKVVPTPGHSIGGVSFIFPEDECVITGDALFRETV
                     GRTDLPTSNFDDLITGIREHIFTLPNHYTVHPGHGQNTSVGHEKNFNPFFRM"
     gene            857..3370
                     /locus_tag="VCA20_002279"
     CDS             857..3370
                     /locus_tag="VCA20_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HEP1836003.1"
                     /translation="MEFVWSFFHEREYAIMNSMKDTRTSNRYAVVDLEATGTGTDAKI
                     IQIGIVLVENGEIIDSYATDINPYELLDDHIKNLTGITDQQLSQAPDFGQVASTIYDM
                     IGDAIFVAHNVKFDANLLAEALFFEGYELLTRRVDTVELSQLFFPTFEKYSLGNLAEH
                     LELGLDQAHTAISDAMATARLLIKIQEKIKSLPRSIVEKILDLADNLLFESRMVIDEM
                     VPVLLENLSYDLESIHDLVLKKPEEIKSAYRLSEDFSTNIALLGLHERKKQTAFAQVV
                     EKRLVDEEKVHFIQAQAGLGKTYGYLLPILAKSEQPLLVTVPTKLLQEQIMEKEGSKL
                     KEIFRIPVVSLKSPKHFIKLDNYWKTLQRQDDNRLVNQFKMQVLVWLCETTTGDLDEL
                     KQKQRYQAYFDEIAHDGKLEPESLFWGWDFWQQLNQQALSSRLLITNHAYFLSHLKDQ
                     DPLMDKRLVVIDEAQKFLLAAENLATASQDLTSMLQVLQSKKDRATTILDQRLYESCQ
                     FELNHFLSRFRQHGQREVQKAELHQLGQNLEELGDVDLNDLHQLIDYYDTFWLEEKNL
                     EEKRLAYLRGSKDSLLNATNYLPDTKIFCISATLTISKKVSLADLLGFEQVTLDLIPT
                     QTVTNQRLLFPTHLPDILAWTKEEHAAYLATTLGEIAELGRPILVLFTSISLLLQVSE
                     LLEDNNIPHLAQHKHGQEMPLKRKFERGECQILLGTGVLWEGVDFASQNQLIQVITRL
                     PFDNPKDRFVQKIKHHLREEGKNPFYDYSLPMMMIKLKQAIGRTNRHDKQDSLVLVLD
                     PRIHTKRYGQQILSFFEKDYSMLSVDAKEIEGAIQDFFE"
BASE COUNT         1092 a          723 c          826 g         1004 t
ORIGIN      
        1 aatggcaagt agtacgaatg cctctttaag aggctagtga cgagtcaaaa gcagtgaggc
       61 ttgaacaaag tgaaagccag cgtctttagg cactggctgg tgatgtgggc ttatagccct
      121 tgttcaaacc acccgtttga cgggtggtca tgattttaca ttctaaaaaa tggattgaaa
      181 tttttctcat gtccaacgct ggtattttga ccgtgacctg gatggacagt atagtgattt
      241 ggcagggtaa agatatgttc tctgatacca gtaatcaggt catcaaagtt gctggttggc
      301 aggtctgtgc gtcccacagt ttcacggaaa agtgcgtctc cagtgatgac acattcatcc
      361 tctgggaaga taaaggaaac accaccaata gagtgcccag gtgttggaac taccttgaaa
      421 tgaaaaccgt caaactggta gtcttcttgg tattggaaga ttttttcagc aggtcggcag
      481 actacatttt ccatatcatc atgacgaggt agacctgata gattcatctc tggtatgtag
      541 agccatctgg cttcactttc agccacatag acaggcggga aattgtaggt ctcacgcaga
      601 atgtccaaac ttataatatg gtcatagtga gtgtgagtca ggagaatagc cgaaattggt
      661 tttccgattt tttcgatagt tacttgaatt tttgtccagt cactcccagg gtcaatgata
      721 atcagatggg tatcattttc aaggtagtag gtattttgaa aagcgacagg attgacagat
      781 ttgtgaataa tcattctttt tccttcttct aacagttact gatgttacta gtctaacaaa
      841 aaaacacgcc ttcttcatgg aatttgtttg gagctttttc catgagaggg aatatgctat
      901 aatgaacagt atgaaagaca caagaacaag caatcggtat gccgttgtag atttggaagc
      961 aactggtaca ggtaccgatg caaaaatcat tcaaatcggg attgttctcg ttgaaaatgg
     1021 agaaatcatt gacagttacg caacggacat caatccctat gagctactgg atgaccatat
     1081 caagaatctg acaggtatca cagaccaaca gctaagtcag gctccagatt ttggtcaggt
     1141 agcaagtacc atttacgaca tgattggaga tgctattttt gtagcccata atgtcaagtt
     1201 tgatgccaat ctcttggcag aagcactctt ttttgaaggc tatgaactgt tgacacggcg
     1261 tgtagatacg gtagaattat cacagctatt ttttccgact tttgaaaaat atagcttggg
     1321 aaaccttgca gagcatttgg aacttggttt ggaccaggca cacacggcca tatcagatgc
     1381 catggctacg gcgcgcttgc tcatcaaaat tcaagagaaa ataaagagct tacccaggtc
     1441 tattgttgaa aagattcttg atttggcgga caatctgctc tttgaatccc ggatggtcat
     1501 tgatgagatg gtgcctgttc tgttagaaaa tctatcatac gatttagaat ctattcatga
     1561 cttggtcttg aaaaagccag aagaaatcaa gtcagcctac cgattatctg aagatttttc
     1621 gaccaatatt gcccttctcg gcttgcatga acgaaaaaaa caaactgctt ttgctcaagt
     1681 agtagaaaaa cgcttggttg acgaagaaaa agtgcatttt attcaagcgc aggcaggttt
     1741 ggggaagact tacggttatc tcttgcctat acttgctaag tcagaacagc ctttattggt
     1801 caccgtaccg accaagctcc ttcaagagca gattatggaa aaggaaggga gcaaacttaa
     1861 agagatattt cgtattcccg tagtctctct aaaatcgccc aagcatttta tcaagttaga
     1921 caactactgg aaaaccctgc agaggcagga tgataatcgc ctggtcaatc aattcaaaat
     1981 gcaggtgctt gtctggttat gcgaaaccac gacaggtgat ttggatgagc ttaagcaaaa
     2041 acaacgctat caagcttact ttgatgagat tgcccacgat gggaaattgg agcctgaaag
     2101 tcttttttgg ggctgggatt tttggcaaca attgaaccaa caagctctct ctagtagact
     2161 gctcattacc aaccacgctt actttctcag ccatcttaag gatcaagatc ctctcatgga
     2221 taagcgtctg gtggtgattg atgaagcaca aaagtttctc ttggctgctg aaaatctagc
     2281 gacggcttcg caagatttga caagtatgtt acaagtattg caaagtaaaa aggacagagc
     2341 gacgactatt ttggaccaac gtttgtatga gtcgtgccag tttgaactca accacttttt
     2401 gagtcgtttt cgtcagcatg gtcaacgaga ggttcaaaag gctgaacttc atcaacttgg
     2461 tcagaacctg gaggagcttg gagatgttga tttgaacgac ttgcatcagc tcatagacta
     2521 ttacgatacc ttttggttgg aggagaaaaa tttagaagaa aagcggcttg cctatttgcg
     2581 gggctccaag gacagcctcc taaatgcgac caactacctt ccagatacta agattttctg
     2641 catcagcgcc accttgacca tcagcaaaaa ggtcagtcta gcggatctgc tgggctttga
     2701 gcaggtcacc ttggatttga taccgactca aactgtgacc aaccagcggc tactttttcc
     2761 gacccatctc cctgacatcc tcgcttggac caaggaggag catgcagctt atttggctac
     2821 tactttagga gaaatagcag agctgggacg accgattttg gtcttgttca catcgatttc
     2881 tctgctcttg caggtgtcgg aattgttaga agacaataac attccccacc tagcccagca
     2941 caagcatgga caggaaatgc ctctcaaacg caaatttgaa cgaggggaat gccaaatttt
     3001 actcggcaca ggtgtattat gggagggcgt tgactttgcc agtcagaatc aattgattca
     3061 ggtcattaca agactgccat ttgacaatcc aaaggaccgg tttgtgcaaa aaatcaaaca
     3121 ccatctgcgt gaagaaggga aaaatccatt ttacgattac agcctgccta tgatgatgat
     3181 taagctcaaa caagccatcg gtcgtaccaa tcgccatgac aaacaagatt cactagtctt
     3241 ggttttggat ccgcgtatcc ataccaaacg ctatggacag caaattctgt ccttctttga
     3301 gaaagattat tcaatgttaa gtgtggatgc gaaagaaata gaaggagcta ttcaagattt
     3361 ttttgaataa gttgaaaggg agtgcgaaag aactcgactg gtcaaaaaca tactaagatt
     3421 agtagtgaag aaatctccga cgggagaaag tagtcactac tttttcttta tgataaagta
     3481 gaggtgtctt gttaagtcga gaactctttt gacgctagac gtcgcatcaa aaactggcaa
     3541 ggcacctgtt ttagaaagaa tgttatcaaa gtatgtggga cgccagacgt cgagtattga
     3601 aaatgacatc gctaaaacaa ggaatcattc aagacaaaga ggtaa
//
LOCUS       DATUCG010000179         3638 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.182, whole genome shotgun sequence.
ACCESSION   DATUCG010000179 DATUCG010000000
VERSION     DATUCG010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3638
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.182"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            <1..262
                     /locus_tag="VCA20_002280"
     CDS             <1..262
                     /locus_tag="VCA20_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017284794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEP1836004.1"
                     /translation="NPYLDIDYFAQRTFNHGMKRLTGRFKLVWKKQDGRCHHCGLPME
                     LGEDREIFFKVPKSKGGVEEVDNMAYVHSYCQRLFIESRSKE"
     gene            267..1229
                     /locus_tag="VCA20_002281"
     CDS             267..1229
                     /locus_tag="VCA20_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000892893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein VirB4"
                     /protein_id="HEP1836005.1"
                     /translation="MLEPYEGKLSRTVLREEGGSNTTNLLDYSIIGQAFGGESIDIAP
                     DSTTFLNVLDLSDENMDEDPVKVKSEFLLSWIGKLLDRKMDGREKSLIDRVTRLTYKH
                     FDTPSLVEWVFVLAQQPEQEAKDLALDMELYVEGSLDIFSHRTNIKTDSHFLIYNVKK
                     LGDELKQIALMVIFDQIWNRVVKNQKLGKKTWIYFDEMQLLLLDKYASDFFFKLWSRV
                     RKYGAIPTGITQNVETLLLDANGRRIIANSEFMILLKQAKSDREELVHMLGLSKELEK
                     YLVNPEKGAGLIKAGSTVVPFKNKIPQHTKLFDIMSTDPEKMRT"
     gene            1231..>3638
                     /locus_tag="VCA20_002282"
     CDS             1231..>3638
                     /locus_tag="VCA20_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEP1836006.1"
                     /translation="MKEDKKLVKQARQNFRSNLKSARMHYRKEVRALKQTVPKKGRFR
                     KSAKNSLFQGKKTELKGNLLSSQKEAKEKFLKEITYVSPRLLKLKEIKYYRLPQAQER
                     LRTARKHLSEVKLSEKQKAVNPKFTFQKENPSLKSRFQFHQEKSFDRLGAEKDISSAK
                     REVKQLKKVQKSKKNSTKVKVGLGLAASESLDLVAQDDDLDGLRTLKDTSLKARRYGR
                     FTFQAGKVAVKSGRTGVRFTKTKFSHGKERFQNFKKGKGFTRQQPLKPRRRYQTFLKH
                     ARKQSVAGFKGIVQAIKGSMTFFSVLAGNPLTWIVSGILLILLLMMSFFMSVSGSSVL
                     QQDEMELTKAYTHMMWEDAEHTRSNEKGITYYTKIDEVMVYMNHQYQDYKLDDFMETG
                     GTTYKAFLSQVWTDLNGGDSIKSMSDLYKEPSYKLSDEDQEELKELTEEGNYLALQEL
                     DNPFQGQTDEDSLNMTYRYGYEVIDEKPTLHHHIILEAKEGQVIVSPMDGKVSLDGEN
                     IILTSGKGVNKTKLTLFGIHSGRVSEHQQVLAGDIIGETKDGTGLKVTYQKVDDDTDK
                     LVYVNPAFYFPKVIQVQTTILPTIGQFGGDEFERAKAIYDYLKSKGATNQAIAAILGN
                     WSVESSINPKRAEGDYLSPPIGATDSSWDDEGWLSLNGPTIYNGRYPNILKRGLGLGQ
                     WTDTVDGSRRHTLLLEYAKGKHQKWYDLGLQLDFMLHGDSPYYTNWLKDFFKNSGSPA
                     SLAQLFLIYWEGNSGDKLLERQTRASEWYYQIEKGFSQPNGGTAQSDPKALEAVRGDL
                     FENS"
BASE COUNT         1169 a          693 c          824 g          952 t
ORIGIN      
        1 caatccttac ttggatatag actattttgc tcaacgaacc tttaatcatg gtatgaagcg
       61 tttgacaggt cgttttaaac ttgtttggaa gaaacaagac ggacgctgtc atcactgtgg
      121 tctcccaatg gaacttggag aagatagaga aattttcttt aaagttccaa aatcaaaggg
      181 aggtgtggaa gaagttgata atatggctta tgtgcatagc tactgtcagc gactatttat
      241 tgagagccgc tcgaaagagt gatgagatgc ttgagccgta tgaggggaaa ctctcacgta
      301 cggttcttag agaggaaggt ggtagtaata ccactaacct actcgactac agtattatcg
      361 gtcaagcctt tggtggggag agtattgata ttgccccaga ttctaccacc ttcttaaatg
      421 tcttagatct atctgatgag aatatggatg aggatccggt taaggtcaag tccgaatttc
      481 ttctctcttg gattggaaag ctcttggacc gtaaaatgga tggtcgcgaa aagtcgctga
      541 tagaccgtgt gacacgactc acctataagc attttgacac accatccttg gtcgaatggg
      601 tctttgtctt agctcaacaa cctgaacagg aagctaagga cttggcactc gatatggaac
      661 tctatgtgga agggtcgctg gacatatttt cacatcggac caatattaaa acagacagtc
      721 atttcctgat ttacaatgtc aaaaagttag gcgatgaatt aaaacagatt gccctcatgg
      781 ttatctttga ccagatttgg aatcgtgtgg taaaaaacca aaaactaggg aagaaaacct
      841 ggatttactt tgatgaaatg cagctactct tattggacaa gtatgcctct gatttcttct
      901 tcaaactttg gagtcgtgtc cgtaagtatg gggcgatacc gacaggtatt actcagaatg
      961 tagaaacgct tcttttggat gccaatggca gacgcattat tgccaatagc gagttcatga
     1021 ttcttttaaa acaggctaag agcgatcgcg aagaattggt gcatatgctt ggactttcca
     1081 aggaactgga gaaatacttg gtcaatcctg aaaaaggggc tgggctaatc aaggccggtt
     1141 caaccgttgt tcctttcaaa aataagattc cacaacacac caagctcttt gacatcatga
     1201 gtacggaccc tgaaaaaatg aggacatgag atgaaagagg ataaaaagct agtcaaacag
     1261 gctaggcaaa actttcgaag taacttaaaa tcagcccgta tgcattatcg gaaagaagtt
     1321 agggccttaa agcagactgt tcctaaaaaa ggaagatttc gaaaatcagc caaaaattct
     1381 ctgtttcagg gaaagaaaac agagttaaaa ggaaatctac tcagtagcca aaaggaagca
     1441 aaggagaagt ttctaaagga aattacctat gtgtctccta gactgttgaa gttaaaggaa
     1501 atcaagtact atcgacttcc tcaagctcaa gaacgtttgc ggacggcaag aaaacattta
     1561 tcagaagtga aactaagtga gaagcaaaag gcagttaatc ccaagtttac tttccaaaaa
     1621 gaaaatcctt ccctgaagtc tcgctttcag tttcaccaag aaaaatcatt tgatcggcta
     1681 ggtgcagaaa aagacataag ttctgccaag cgtgaggtta aacaactcaa gaaagtccaa
     1741 aagtctaaga aaaactctac caaagtcaaa gttggattag gcttagctgc atctgaatcg
     1801 cttgacctgg tagcacagga tgatgattta gatggtctaa gaaccttaaa ggatactagc
     1861 ttaaaagcca gacgctatgg caggtttacc tttcaagcag gtaaggtggc agtaaaaagt
     1921 ggtcggactg gtgtgcggtt taccaaaaca aaattttctc atggaaagga acgattccag
     1981 aacttcaaaa agggaaaagg attcacacgc cagcaacctc ttaaaccaag aagacgctac
     2041 caaacctttt taaagcatgc cagaaaacaa agtgtcgcag gtttcaaagg aatcgtccaa
     2101 gccattaagg gaagtatgac tttcttttct gtccttgcgg gaaatccttt gacttggatt
     2161 gtctcaggca ttctcttgat actccttttg atgatgagct ttttcatgag tgtctcaggt
     2221 agcagtgtcc ttcaacaaga tgagatggaa ttgaccaagg cctataccca catgatgtgg
     2281 gaagatgcgg agcatactag atccaacgaa aagggaatta cctattacac caagattgat
     2341 gaggttatgg tttacatgaa tcatcaatac caagactaca agcttgacga tttcatggaa
     2401 acgggtggta cgacctacaa agcatttctc agtcaggtgt ggacggactt aaatggtgga
     2461 gattctatta aatccatgtc tgacttgtat aaggaacctt cttacaagct gtctgatgag
     2521 gaccaagaag aactaaagga gttgaccgaa gaaggcaact atttagccct tcaagaattg
     2581 gacaatccct ttcagggaca gaccgatgag gatagcttaa acatgaccta ccgatatggt
     2641 tatgaggtca ttgatgagaa accaacgctt catcatcata tcatcttaga agcaaaagaa
     2701 ggtcaagtca ttgtgtctcc tatggatggt aaggtatctc ttgatggaga gaatattatt
     2761 ttgacatctg gtaagggagt gaataagact aaactaacct tgtttggcat tcattcaggc
     2821 cgagtgagtg aacatcaaca agtcttggca ggagacatta ttggtgagac caaggatgga
     2881 acaggtctaa aagtcaccta tcaaaaagtt gatgatgaca cggataagtt ggtctatgtc
     2941 aatccagctt tctactttcc aaaagtgatt caggttcaga ccaccattct tccaactatc
     3001 ggtcagtttg gcggcgatga gtttgagaga gccaaggcaa tttatgacta tcttaaaagc
     3061 aaaggtgcga ccaatcaagc tatcgcagcc attctaggta actggtcggt agaatcctcc
     3121 atcaacccga aacgtgccga gggcgactat ctatctcccc ctattggtgc gacagacagt
     3181 tcgtgggatg atgagggctg gctctcactt aatggtccaa ctatatacaa tggacgttac
     3241 ccaaatattc tcaaacgtgg tttaggctta ggacaatgga cagataccgt ggatgggtca
     3301 cgcagacata ctttattgtt ggaatatgcc aaaggaaaac atcaaaaatg gtatgactta
     3361 ggcttacaac tggatttcat gttgcatggg gatagtcctt attacaccaa ctggttaaag
     3421 gacttcttca aaaattcagg aagtccagct agtcttgccc aactctttct catttactgg
     3481 gaaggaaata gtggtgataa gctacttgaa cgtcagacac gagcaagtga gtggtattac
     3541 caaattgaaa aaggctttag tcaacctaac ggtgggacag cacaaagtga tccaaaagca
     3601 cttgaagctg tacgaggaga cctttttgag aactctat
//
LOCUS       DATUCG010000180         3508 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.183, whole genome shotgun sequence.
ACCESSION   DATUCG010000180 DATUCG010000000
VERSION     DATUCG010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.183"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(904..1113)
                     /locus_tag="VCA20_002283"
     CDS             complement(904..1113)
                     /locus_tag="VCA20_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1836007.1"
                     /translation="MAKPKEKWTPIITHIVPKGTELADGTILDKETTLSQEEFSKNPP
                     VIPDGHPFYNIWARIIREKIEKGEL"
     gene            complement(1225..1677)
                     /locus_tag="VCA20_002284"
     CDS             complement(1225..1677)
                     /locus_tag="VCA20_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEP1836008.1"
                     /translation="MLVTYPALFYYDDTDGANAPYFVTFPDFEHSATQGEDMADAMAM
                     ASDWLGINLADYIENGRDIPTPTPINALSLVDNNPFRDDEDIELIYDPSKSFISMVMV
                     DVAEYLGSQEPVKKTLTIPRWADTLGRELGLNFSQTLTDAIADKKIHA"
     gene            complement(1716..1898)
                     /locus_tag="VCA20_002285"
     CDS             complement(1716..1898)
                     /locus_tag="VCA20_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002961998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEP1836009.1"
                     /translation="MPMTQKEMVKLLTANGWTKTKGGKGSHVKLEKAGERPITIPHGE
                     INKYTERGIKKQAGLL"
     gene            complement(2086..2472)
                     /locus_tag="VCA20_002286"
     CDS             complement(2086..2472)
                     /locus_tag="VCA20_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEP1836010.1"
                     /translation="MFEINMTINERLRDIRDLKDAVSSLENDKLELEKTYPVQSRRIR
                     KRKARLLVAIRGIKAKRQRMTDLINQLSDENQRKILTLQYIEGVKDKHLVEVSGLKDY
                     REVSSIRQKAIKNLERLQKQLEQPQA"
     gene            complement(2607..3107)
                     /locus_tag="VCA20_002287"
     CDS             complement(2607..3107)
                     /locus_tag="VCA20_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1836011.1"
                     /translation="MIELTIESITKPLKQKRLSRVSGKLKGVGITIDLNNLTVNYGSQ
                     QINLASIPGTYGGVRYFFLCPDCGRRCRKLYKQYLYFGCGMCQQVYKKTLNRSKTDCQ
                     YYFELAFKEARKIDPSYYPQKGYVDYDNFPSRPKRMRAKTYWKHYRKFTSYLDKGVSY
                     WLGGLK"
     gene            complement(3177..>3508)
                     /locus_tag="VCA20_002288"
     CDS             complement(3177..>3508)
                     /locus_tag="VCA20_002288"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1836012.1"
                     /translation="KLMEIAKKLQAEDTSLNIYELYKHPEARAKLFSQITEACYMALN
                     ATPTQAQRLAFCDYLEQQYENTLKKMVASTDKQALGELLDLLELPVEIESQFIRDMAI
                     SGLLAKG"
BASE COUNT          979 a          687 c          627 g         1215 t
ORIGIN      
        1 ttataaatca aaataaagag cataacacct tttcattcag tgttgtgcct tgagtgaaac
       61 gaaaggaata aattataacc atgacatttg aagagatttt ggaaaatgtc taggaatggc
      121 ttgaaatagg cgtttatagc ctctgaattt gtggactttt tgaaaaaaca tagtcacaaa
      181 tgacctttaa tattctccca aagtccacaa aaaggggagt aaaaaaagac ctaaaatagg
      241 tcaataattt acgagttcag caggcaggaa ctagcacccc acaaggtgct tttttcaggt
      301 cttcttaatt accatacaaa aaagagggat acccctcctc actatcccct caaccaccaa
      361 caggggacac cagcaaaact aacgacatat tggctggtac gtttatcact gagcggcatt
      421 caatgtcgca ctatattccg ctcctttgct atcagaatag cggtggaacc tgtaaaggat
      481 actatatttt catgctatca tattcacttt acaaaagcaa atcttaagta cacttgataa
      541 ggtgcttttt agttatctga tgtcttgctg atagctattt tataccaaaa aagggagtgt
      601 atgacctcac gggttaactc tccctaatcg gctagactat gcaagcctcc agctgtgaat
      661 atattatacc acggatagct ggcacaagtt agttatttct cacaatatgc catttttaaa
      721 agatagataa ctgtttccaa attggaaaag gttaaaactt taccgtattg ggattttatc
      781 aaccgtaaat taagtagctt aaatgcgcgt ggttatcaat tttgttgacc ttgacaaatc
      841 aatttcaagc aattatcacg cgcaaatcta ctgtatttta ctgttttcta ctgtatcttg
      901 gtgctatagc tcaccctttt ctatcttctc tctaattatc ctagcccaaa tattataaaa
      961 aggatgtccg tctggaataa cagggggatt tttagaaaat tcttcttgtg ataaggtggt
     1021 ttctttgtct aatattgtgc cgtctgctag ttcagtacct ttagggacaa tgtgggttat
     1081 tattggtgtc catttttctt taggtttagc catgttaaaa ttactccttt attgtgtaaa
     1141 aatacaaaat agggctaacc ttacacctta gcagttatct gttttagtgt actggtttag
     1201 ccctatcata taagttagat agtgttaagc atgaattttc ttatctgcaa tagcgtctgt
     1261 taaggtctgt gagaaattta gccctaactc acgccctaaa gtatccgccc aacgtgggat
     1321 agttagggtt ttctttacag gttcttgact acctagatac tctgccacat caaccataac
     1381 catagagata aaagacttgc tagggtcata aattagctct atatcctcat catcacggaa
     1441 agggttatta tcaaccaaag ataaagcgtt gataggggta ggggtgggaa tatctcgccc
     1501 attctcgata tagtccgcta ggttaatacc taaccagtcg ctagccattg ccatagcgtc
     1561 agccatatct tcgccttggg ttgctgaatg ttcaaagtct ggaaaagtga caaaataagg
     1621 ggcgttagct ccgtctgtat cgtcataata aaataaagct ggataagtga caagcatagt
     1681 ttacctccaa aataaagaca agcgggggac tgtctttata acagccctgc ttgctttttg
     1741 ataccccttt cggtgtattt gttaatttca ccgtggggaa tggttatagg tcgctcgcct
     1801 gctttttcaa gtttaacgtg tgacccttta cctcctttgg tctttgtcca accgttggcg
     1861 gtaagaagtt taaccatttc tttttgtgtc ataggcatag cgctcttacc tcctgacaag
     1921 attattatat catacgtgta acacgtattc aagtaaagat tgttattttt aaatcaattt
     1981 ttatgtaaat caaaaatagc ccctaaaatc gtttctaagg gcttttagtt gctgtatggt
     2041 taatttatcg gataaagaaa aacacgcgct aaaatagggc taaatttaag cttgaggctg
     2101 ttctagttgc ttttgtaacc gctctaagtt cttaatagct ttttgtctaa tgctagagac
     2161 ttctctataa tcttttagac cgcttacctc tactagatgt ttgtctttta ctccttcgat
     2221 atattgtaag gtcaaaattt tacgttggtt ttcgtctgat agttgattga ttaagtctgt
     2281 catacgttgc cgttttgctt taattcctcg tattgctacc aataatcttg cttttctctt
     2341 tctaattctc ctagactgta ctggataagt tttctcaagt tcaagtttat cattttctaa
     2401 actagaaaca gcatctttca aatctctaat gtctcttagt cgctcgttaa ttgtcatatt
     2461 gatttcaaac ataatttaat acctcgctat gattatatca aaaatagcgc gtgttctttg
     2521 ttcgttcttt gttcgattga gtaagtttat ttgttttggg aactaacaga gcgttcttta
     2581 tttgtttgtt ttgtaaatgc accctgttat tttagtccac ccaaccaata gctcacccct
     2641 ttatctaaat agctagtaaa ctttctgtaa tgtttccagt atgtcttagc cctcattctt
     2701 tttggtctgc taggaaaatt gtcataatca acgtatcctt tttggggata gtaactaggg
     2761 tctatctttc tagcctcttt gaaagctagc tcaaaataat attgacaatc tgttttactt
     2821 cggtttagtg ttttcttgta gacttgttga cacatcccac aaccaaaata cagatattgc
     2881 ttataaagtt tcctgcaccg cctaccacaa tcaggacaaa gaaagaagta gcggacacct
     2941 ccataagtgc ctggtatact agctaggttt atctgttggc taccgtagtt aacagttagg
     3001 ttattaaggt caatagtgat acctactccc tttaacttac cggatactct tgataagcgt
     3061 ttttgtttca atggtttggt tatgctttct atggtcaatt caatcatttt cccctccctt
     3121 attggcaaaa cccaaattta ttgattaaac aacaaaagag gcgcgtgcct ccctgttcag
     3181 cctttggcta gtaacccact gattgccata tctcggataa actggctttc tatctctact
     3241 ggtagctcta acaaatctag taactcacct agtgcctgtt tgtctgtgct ggcaaccatt
     3301 ttctttaagg tgttttcgta ttgctgttct agatagtcgc aaaatgccaa tctttgggct
     3361 tgtgtcggtg tagcattcaa agccatataa caagcttcag ttatctgtga gaatagttta
     3421 gcacgcgctt ctgggtgctt atacagttca taaatgttca gactggtatc ctctgcttgt
     3481 aactttttag ctatttccat tagtttag
//
LOCUS       DATUCG010000181         3480 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.184, whole genome shotgun sequence.
ACCESSION   DATUCG010000181 DATUCG010000000
VERSION     DATUCG010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3480
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.184"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            291..1367
                     /locus_tag="VCA20_002289"
     CDS             291..1367
                     /locus_tag="VCA20_002289"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEP1836013.1"
                     /translation="MTEKTIVFKVGTSSLTQENGSLDRIKIARITNQLAQLYQKGYQI
                     VLVTSGSIAAGFRRLGFDKRPTKIAEKQASAAVGQGLLIEEYTQNLMKDGIVSAQILL
                     TQDDFADARRYQNASQALQVLLKQRAIPIINENDTIAIEEIKVGDNDTLSAQVASLLK
                     ADLLVLLTDVDGLYTANPNSDPTAQHLPQIKEITEDLFAMAAGAGSSNGTGGMTTKLQ
                     AAQIATKSGVPVFICSSKEDTALLQAVTQANRGTLFLADDHAMNQRKQWMAFYARTDA
                     AVEVDAGAVDAMLHQGRSLLATGVKALEGDFEVGQVVEVYSQADHRLIGKGRVKLSSK
                     DLQDQLANGRAEGVFIHRNDWVSL"
     gene            1432..2670
                     /locus_tag="VCA20_002290"
     CDS             1432..2670
                     /locus_tag="VCA20_002290"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEP1836014.1"
                     /translation="MTTTQVLLDSLLAHKASINLATTEQKNQALSAMADQLVAQTEAI
                     LAGNAIDMENAQGKISQVMQDRLLLTAERIEAMADGIRALIGLPDPVGLVLEESTRAD
                     GLNICKKSIPFGLAGMIYESRPNVTSDAAALAIKSGNAVILRGGKEAFHSAQAIVTAL
                     KAGLEEVGLSPKVIELVQDTSHASATELMTAQGKIDLLVPRGGAGLIQAVVENATVPV
                     IETGTGICHVYVDKDADLDKALRIVVNAKTSRPSVCNAAEVLLVHEEIASQFLPRLEE
                     ALSGQVELRADSQAQALLNQARPAGDQDFDTEFLDYIMAVKVVSSVEEAISHIAQHST
                     GHSEAIVTENSQTAEHFTLHVDSAAVYVNVSTRFTDGGEFGLGCELGISTQKMHARGP
                     MGLREMTTYKYIITGDGHIR"
     gene            2680..3465
                     /gene="proC"
                     /locus_tag="VCA20_002291"
     CDS             2680..3465
                     /gene="proC"
                     /locus_tag="VCA20_002291"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEP1836015.1"
                     /translation="MKIGFIGLGNMGAALAQAVSQQPDTQLLLSNHNPAKAQALQEQL
                     GGQLFSNGEIAEQAEIIFLGVKPHLIQTVLSGLQDQISQNPSAIWISMAAGVPLDSLA
                     EFVLADKLIRIMPNTPVAIGQGMTTYSLVNPELAPLLEQILEKSGKVLQVPESLIDAA
                     TAIAGCGPAFVYQMIEALTDAGVENGLTAQDAKLLAAQTLAGTAQMVLTSDKHPAQLR
                     QEVTSPGGSTIAGVVALEKEGFRYAIIKAVAAALKKTRKLGKK"
BASE COUNT          937 a          821 c          897 g          825 t
ORIGIN      
        1 cagatttgga gtgtgaaaca cgaattgctg agatttgctt cgcaaatcct atctccaacc
       61 tttaacagtc cactggattg ttaaacccaa tcaaccactg cgctgagatg ttgacacgaa
      121 ctctgagtag tagccactgg gctttttgcc cagcctcttt gtttccagcg ttttttaatg
      181 cactcatgat taggaatcta ttccctctct ccttgccgaa taacagtgat tttctttatg
      241 attaaaatat ggtataattt tccaaatatt agtttttgtg agaagagatt atgacagaaa
      301 aaacaattgt atttaaggtc gggacaagtt ctctgacgca agaaaatggg agtttggacc
      361 gtatcaaaat cgctcgaatt accaatcagc tggctcagtt gtatcagaag ggctaccaga
      421 ttgtgctggt gacgtcgggc tcgattgctg ctggttttcg gagattggga tttgacaagc
      481 gaccgaccaa gattgcggag aagcaggctt ctgcggcagt tggacaaggt ttgctaattg
      541 aggaatatac gcaaaatcta atgaaggacg gcatcgtatc ggctcagatt ttgctgaccc
      601 aggatgattt tgcggatgcc agacgttatc agaatgcctc tcaagccttg caggtcttgc
      661 tgaaacaacg tgctattcct attatcaacg aaaatgatac cattgccatt gaggagatta
      721 aggtggggga taacgatact ttatctgctc aggtggcttc tctcttgaaa gcagacctat
      781 tggtgctctt gacggatgta gatggacttt acactgccaa tcctaacagc gatccgactg
      841 cccagcactt gcctcaaatt aaggagatta cggaagattt atttgccatg gcagcaggag
      901 ctggctcgtc caacggaaca ggtggcatga ccaccaaatt gcaggcggct cagattgcga
      961 ccaagtcagg cgtgcccgtc tttatctgct cttcaaaaga ggataccgcc ctcttacagg
     1021 cggtcaccca agccaatcgt ggaacgctct ttttagcaga tgaccatgcc atgaaccaac
     1081 gcaagcagtg gatggccttc tatgctcgga cagacgcggc ggttgaagtg gatgcaggag
     1141 cggttgatgc catgttgcac cagggccgca gtttgttagc tactggtgtc aaagccctcg
     1201 aaggagactt tgaagtgggc caggtcgtag aggtttatag tcaagcagac caccgtttga
     1261 ttggtaaagg acgggtcaaa ctgtcctcca aggacttgca ggaccagttg gcaaatggca
     1321 gagcagaggg cgtgtttatt caccgcaacg attgggtgag cttatagttg ttcagtttgt
     1381 ctcgattatg ttgacgaacg agtaaactga aagactaaat aggagaaatt tatgacaaca
     1441 acacaagtat tattagatag tttattggcc cacaaggctt ctatcaacct agcgacaaca
     1501 gaacagaaaa accaagcctt atcagcaatg gcagaccagt tggttgctca gactgaggca
     1561 atcctagcag gcaatgccat cgatatggaa aatgcccaag gcaagattag ccaggtgatg
     1621 caggatagat tgctcctgac tgctgaacga attgaagcaa tggcagatgg cattcgcgcc
     1681 ttgattggct tgccagatcc tgtcggatta gtcttggaag aatccactcg ggcagacggc
     1741 ttgaacattt gcaagaaatc cattcccttt ggtttggcgg gaatgattta cgaaagccga
     1801 ccaaacgtga cttcagatgc cgctgccttg gccatcaaga gtggtaatgc ggtcatcttg
     1861 cgtggaggaa aggaagcctt tcattcggcc caggctattg tcacagccct caaagctggt
     1921 ttggaagaag ttggcctatc acctaaggtc atcgagctag tccaagatac cagtcacgcc
     1981 tctgcgaccg agttgatgac tgcccaaggc aagattgacc tcttggtgcc acgtggtgga
     2041 gcaggactca ttcaagccgt agtagaaaat gcgactgtgc cagttattga aacaggaaca
     2101 ggtatctgcc atgtctatgt ggacaaggat gcggacttgg acaaggcctt gcggattgtg
     2161 gtcaatgcca aaaccagtcg tccctcagtc tgcaatgcgg cggaagtctt gttggtccat
     2221 gaagaaattg ccagccaatt cctacctcgc ttggaagaag ctctttctgg tcaggtggaa
     2281 ttacgtgccg acagtcaggc ccaggctctc ctcaaccaag ccagaccagc aggcgaccaa
     2341 gattttgaca cagaattttt ggactatatc atggcggtca aggtcgtttc aagtgtagaa
     2401 gaggctatca gccacatcgc acaacactcg acagggcaca gcgaggccat tgtgacggaa
     2461 aacagccaga cagcagagca tttcacactc catgtggact cggcagcagt ctatgtcaat
     2521 gtctcgaccc gcttcacaga cggtggagag ttcggtctgg gctgtgagtt gggtatttcc
     2581 acccagaaga tgcacgcccg tggtccaatg ggactgcgtg aaatgaccac ttacaagtac
     2641 atcatcacag gcgacggcca cattcgttag gaggacaaga tgaagattgg atttattgga
     2701 ctgggcaata tgggagcggc cctagcccaa gcggtcagcc agcagccaga tacccagctc
     2761 ctcctcagca accataaccc agcaaaagcc caggctcttc aagagcagtt aggcggtcag
     2821 cttttttcta atggggaaat agcagagcag gccgagatta ttttccttgg ggtcaagcct
     2881 cacctgattc agacagtctt gtcaggcttg caggaccaga ttagccagaa tccgtcagct
     2941 atctggattt ccatggcagc aggagtgcct cttgacagcc tggcagaatt tgtgttggct
     3001 gataaactca ttcgtatcat gcccaacaca ccagtcgcta tcggccaagg catgacaacc
     3061 tatagcctgg tcaatccaga gctcgctccg ctattggaac aaatcctaga aaaatcaggc
     3121 aaggtcctgc aagtgccaga gagcctgatc gacgcagcaa cggctatcgc aggctgcggg
     3181 ccagcctttg tctatcagat gattgaggct ttgacagatg caggagtgga aaacggcctc
     3241 acggctcagg atgccaagct tttagcggct cagaccctgg caggtactgc tcagatggtc
     3301 ttaaccagcg ataagcatcc agcccagctg agacaggaag tgacctcgcc aggcggctcg
     3361 actatcgcag gcgtggtagc tcttgaaaaa gaaggcttcc gttacgctat tattaaggca
     3421 gtcgccgccg ccctcaaaaa gactagaaaa ttaggcaaaa aatagaagat agcaaaaaac
//
LOCUS       DATUCG010000182         3454 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.185, whole genome shotgun sequence.
ACCESSION   DATUCG010000182 DATUCG010000000
VERSION     DATUCG010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3454
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.185"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(20..559)
                     /locus_tag="VCA20_002292"
     CDS             complement(20..559)
                     /locus_tag="VCA20_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEP1836016.1"
                     /translation="MPKQRILPHIILGIMGASGQMVTGKQITDYVQRDLGEFWQVAHS
                     QVYPELKRMTKEELITCHAVPGNEKEKQYAMTATGRQILDEWLSIPNEETPQQKDLFS
                     IKMFFIREKDDPRIPGLLQSQIELVTKHLKHLDSRKVELFSTKESIQDNYGHYLILTR
                     SIERNRAQLKWLEDILAEM"
     gene            complement(561..1190)
                     /gene="udk"
                     /locus_tag="VCA20_002293"
     CDS             complement(561..1190)
                     /gene="udk"
                     /locus_tag="VCA20_002293"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEP1836017.1"
                     /translation="MTQKPIIIGVTGGSGGGKTSVSRAILDNFPNARISMIEHDSYYK
                     NQSHLTFEERILTNYDHPLAFDTDLMIYHISELLAGRSVDIPIYDYTQHTRSEKTYRQ
                     EPQDVFIVEGILVLEDKRLRDLMDIKIFVDTDDDIRIIRRIKRDMEERGRSLDSIIEQ
                     YTSVVKPMYHQFIEPTKRYADIVIPEGVSNVVAIDLINTKVESILRERG"
     gene            1273..2259
                     /locus_tag="VCA20_002294"
     CDS             1273..2259
                     /locus_tag="VCA20_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEP1836018.1"
                     /translation="MLNFGIVGTSDISHRFIEAAHLSGHYQLQAVFSRKLETAAAFSE
                     QYEGVDLYTEWVKFISAPIDVVYIASPNALHFEQAKAVLAAGKHAIVEKPMVSTPQEL
                     EQLRRVAQENNVFLFEAARNYHEQAQAIIREFLTDKTILGGSFGYAKYSSKMPELLAG
                     QMPNIFSTDFSGGALMDLGVYTLYAAIGILGTPRTARYVAQQLPSSIDLNGSGQLIYD
                     NNIVHIQAGKNITSNLASEIYTNDGTLTLNACQYISSAIFTKHDGEQIVLPIQAVQHP
                     MLEEALEIAQAIKQQNRKLVDQWLAVAESVHQTLYTMRQDAGIIFKADNNEI"
     gene            2249..3331
                     /locus_tag="VCA20_002295"
     CDS             2249..3331
                     /locus_tag="VCA20_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEP1836019.1"
                     /translation="MKSKFPSSWTDQLTLLGFEDFTPIQVQAFEPIANGKSLLAISPT
                     GTGKTLAYLWPSLLALTPKKAQQLLILAPNTELAGQIFDVCKTWSETIGLTAQLFLPG
                     SSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMNINTIVLDEFDQLFSDSQYQFV
                     EKIINYVPRDHQLIYMSATAKFDRQKIAEDIESIDLSEQKLDNIQHCYMMVDKRERLE
                     TLRKFANIPDFRALAFFNSLSDLGASEDKLLYNGVNAVSLASDVNVKFRKVIIERFKN
                     HELNLLLATDMVARGIDIDNLECVLNFEVPFDQEAYTHRSGRTGRMGKEGLVITLISS
                     PSELKQLKKYASVQEVILKNQELYKI"
BASE COUNT         1025 a          764 c          659 g         1006 t
ORIGIN      
        1 gattcggaaa tccgaatcgt tacatttctg ccagtatatc ctctaaccat ttgagctggg
       61 cacggttgcg ttcaatagac cgtgtcaaaa tcaaatagtg tccataattg tcttggatac
      121 tttccttggt tgagaacagc tcaactttac gactgtctag gtgttttaag tgcttggtga
      181 ctagttcaat ttgactctgc aacaaaccag gaatccgtgg gtcatccttt tcacggataa
      241 aaaacatctt gattgaaaac aagtcttttt gctgaggcgt ttcttcattt ggaatggata
      301 gccattcgtc taaaatctga cgaccagtag ccgtcatcgc atactgcttt tccttttcat
      361 ttccaggtac tgcatgacaa gtaatcaatt cttccttggt catacgtttt aattctggat
      421 agacttggct atgggcaacc tgccaaaatt cgcctaaatc acgttgaaca tagtcggtaa
      481 tctgcttgcc agttaccatt tgaccgctag cacccataat ccctaatatg atatgaggta
      541 atattctttg cttcggcatc ttaaccacgc tctcttaata tactctccac cttggtgtta
      601 atcaagtcga tagctacgac attgctgacg ccttcaggaa tgacgatatc tgcatagcgc
      661 ttggttggct cgataaactg gtgatacatt ggtttcacca ccgaggtgta ctgctcaatg
      721 atactatcta agctacggcc acgctcttcc atgtctctct taatgcggcg aatgatacga
      781 atatcgtcat cagtatcgac gaatatctta atatccatta aatcccgcaa ccgtttgtct
      841 tccaatacta aaataccttc cacaataaat acatcttgtg gttcctgacg ataggtcttc
      901 tctgaacgtg tgtgctgagt gtaatcataa atcggaatat ccactgaacg tcctgctaat
      961 agctcactga tatgataaat catcaaatcc gtatcaaaag ccagtggatg gtcataattg
     1021 gttagtatcc gttcttcaaa ggtcaggtgg gactgattct tataataaga atcgtgttca
     1081 atcatagata tacgagcatt tggaaaatta tccagaatgg cacgtgacac acttgtcttg
     1141 ccacccccag atccgccggt tacgccaata ataatgggtt tctgagtcat catcatctcc
     1201 ttctattttc aatactattt taccatgaaa catggtataa tgaaaaggat aacacaaaag
     1261 aaatgagaat ttatgcttaa ttttggtatt gttggcacct cagacatctc tcatcgtttt
     1321 atagaggcgg cccatttaag tggacattat cagctacagg ctgttttctc ccgtaaacta
     1381 gaaactgctg ctgccttttc agagcagtat gaaggagtcg atttatacac agaatgggta
     1441 aaatttattt ctgcccccat tgatgttgtc tatattgcca gtccaaatgc cctgcacttt
     1501 gaacaggcaa aagctgttct agctgctggt aaacatgcta ttgttgagaa gcccatggtt
     1561 tctacacctc aggaattaga acaattgcgt cgcgttgccc aagaaaataa tgttttcctc
     1621 tttgaggctg ctcgaaacta tcacgaacaa gctcaagcca ttatccgtga atttttgacg
     1681 gataaaacga ttttaggtgg ttcatttggg tatgccaaat attcttctaa gatgcctgaa
     1741 ttgttagctg ggcaaatgcc aaatatcttt tcaactgatt tttccggtgg tgctctcatg
     1801 gatttaggtg tctatacgct ctacgccgct atcggcattt tgggaactcc cagaacagcc
     1861 cgctatgtag cacaacagct accttctagt attgacttaa atggttcagg acaactcatt
     1921 tatgacaaca atatcgtcca cattcaagca ggaaaaaata ttacgagcaa tctagcgagc
     1981 gaaatctata caaatgatgg aacactaaca ctcaacgcat gccagtatat cagctctgct
     2041 attttcacaa aacatgacgg agagcaaatt gttttaccaa tccaagcggt ccagcaccct
     2101 atgctcgaag aagcgctaga aattgcacag gcaatcaagc aacaaaaccg taagcttgtg
     2161 gaccagtggt tggctgtggc agaaagcgtc catcaaacac tttacaccat gcgtcaagac
     2221 gcaggaataa tctttaaggc ggataacaat gaaatctaaa tttccctcca gctggacgga
     2281 ccagcttacc ctacttggct ttgaggactt tactcctatc caagtccagg cctttgagcc
     2341 tattgcaaac ggcaagtccc ttctagctat cagcccaaca ggcacaggaa aaacccttgc
     2401 ctacctctgg cctagcctat tagccctaac tcctaaaaaa gcccaacaat tgctcatcct
     2461 cgcaccaaat acagagctgg ctggtcaaat atttgatgtt tgtaagactt ggtcggagac
     2521 aattgggttg actgctcaac tattcttgcc tggttccagt caaaaacgcc agattgagcg
     2581 actaaaaaaa ggaccagaaa tcctgattgg aacacctggt cgtattttcg agttgattaa
     2641 attgaaaaaa atcaagatga tgaatatcaa caccatcgtc ctagatgagt ttgatcagct
     2701 attctccgat tctcaatatc agtttgttga aaagattatc aactatgtac ctcgtgacca
     2761 ccagttaatc tacatgagtg caacagctaa atttgaccga caaaaaattg ctgaagacat
     2821 tgaaagcatt gatttatctg agcagaaatt ggataatatc cagcattgct atatgatggt
     2881 agataagcgt gaacgtttgg aaactctacg taagtttgcc aatatccctg attttcgtgc
     2941 gcttgcattt ttcaacagcc tatcagacct tggtgcaagc gaagataaac tactctataa
     3001 tggtgtgaat gcagtttcat tggcttctga tgtcaacgta aaattccgta aggtcatcat
     3061 cgaacgtttc aaaaatcacg aactcaatct tctacttgct acagatatgg tcgcacgtgg
     3121 tattgatatt gataacttag aatgtgtcct caactttgaa gttccttttg accaagaagc
     3181 ctacactcat cgttctggac gtactggacg catgggcaag gaaggtcttg tcatcaccct
     3241 aatatctagt cctagcgaac taaaacaatt gaaaaagtat gcctccgtcc aagaagtcat
     3301 tctcaagaat caagagctct acaaaatata atgaacaata tacaaaaggc ttagaaccag
     3361 tgtttctact aacatgattc taagcctttt atcattagac ttgttcggta actcaaaagt
     3421 ggtatagtca actgaaacag gagcaggacg aaag
//
LOCUS       DATUCG010000183         3373 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.186, whole genome shotgun sequence.
ACCESSION   DATUCG010000183 DATUCG010000000
VERSION     DATUCG010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.186"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(279..1430)
                     /locus_tag="VCA20_002296"
     CDS             complement(279..1430)
                     /locus_tag="VCA20_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEP1836020.1"
                     /translation="MATFYVPAVNLIGKGVVNEVGPYIKELGYKKALLVTDKFIEGSD
                     ILPKVLKPLDIEGIEYVIFSDVEPNPTCKNVTDGVAALEEHGCDFIISLGGGSPQDAA
                     SCISIIATNGGKPQDYEGLHKSAKKGLPVVAINTTAGTSAEITINYVITDEERKVKMV
                     MVDKNSLALISVNDPELMISKPQALTAATGMDALTHAVEALVTPGAYDVTKKLSIGAI
                     ELIKEYLPRAVANGHDIEAREGMVNAIFLGGMSFNNAGLGYVHSMAHQLGAVYNLPHG
                     VCCAMLLPVIERENTKCVPEAFRNVAKALGLHVEGKSDEECADYAIAEIEKLSETVGI
                     PKKLTELGIEEKDFDFEYLSKNALIDACAPGNPFMPTLEETIEFYKELF"
     gene            complement(1566..3332)
                     /locus_tag="VCA20_002297"
     CDS             complement(1566..3332)
                     /locus_tag="VCA20_002297"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HEP1836021.1"
                     /translation="MIQHPRIGIRPTIDGRRQGVRESLEVQTMNMAKSMADLISSTLK
                     YPDGEPVECVISPSTIGRVPEATASHELFKKSNVCATITVTPCWCYGSETMDMSPDIP
                     HAIWGFNGTERPGAVYLAAVLASHAQKGIPAFGIYGRDVQEANDTDIPEDVKEKLLRY
                     ARAALATGLMRDTAYLSMGSVSMGIGGSIVNPDFFQEYLGMRNESVDMTEFTRRIDRG
                     IYDPEEFERAMVWVKEHIKEGVDRNREDLILSKEEKEKQWEFVVKMFMIGRDLMQGNP
                     RLAELGFEEEAVGHHALVAGFQGQRQWTDHFPNGDFMETFLNTQFDWNGIRKPFVFAT
                     ENDSLNGVSMLFNYLLTNTPQIFADVRTYWSPEAVKRVTGHTLEGNAADGFLHLINSG
                     SCTLDGTGQASRDGKPVIKPFWELEESEVQAMLDNTDFPPANREYFRGGGFSTRFLTK
                     GDMPVTMVRLNLLKGVGPVLQIAEGYTLELPEDVHHTLDNRTDPGWPTTWFAPRLTGK
                     GAFKSVYDVMNNWGANHGAITYGHIGADLITLASMLRIPVNMHNVPEEDIFRPKNWSL
                     FGTEDLESADYRACQLLGPLHK"
BASE COUNT         1009 a          761 c          576 g         1027 t
ORIGIN      
        1 gctcgcaaaa aaagaggtgc tagaggccta tttgccatgg gttaaaaata ttaagagagc
       61 atgtaaattg aaaaaggttc cagagtcgac aggactttgg aacctttttc aatatacctt
      121 gttattgggc gcttaccatc tgcgtcgcct agtctgattt tgattttcat tgagtattaa
      181 tttataaaaa gattgagcat tgtacaatac aaaactcaat ccattaaaca acttatttcc
      241 atctttttaa gttagcacac aattaaactc attatttctt aaaacaactc tttataaaac
      301 tcaatcgttt cttctaaggt tggcatgaat ggatttcctg gtgcgcaggc atcaatcaag
      361 gcattcttag aaaggtattc aaagtcaaaa tctttttctt caataccaag ctcagtcaat
      421 ttcttaggaa tgcctactgt ctcagaaagc ttctcaatct cagcaattgc ataatcggca
      481 cactcttcat ctgatttacc ttctacatga agtcccaagg ccttggcaac attacggaaa
      541 gcttctggta cacatttagt attttcacgt tctataactg gtaacaacat ggcacagcaa
      601 acaccgtgtg gcaaattata tactgcaccg agttggtgag ccattgagtg aacatagccc
      661 agacctgcat tgttaaagct cataccacca aggaagatgg cattgaccat accttcacgc
      721 gcttcaatat cgtgtccatt tgctacagca cgaggaaggt attccttgat aagctcaatt
      781 gctccgatag ataatttctt ggtcacatca taagcaccag gtgttaccaa agcttcaaca
      841 gcgtgagtca gagcatccat accagtagca gcagtcaaag cttgaggttt tgaaatcatc
      901 aattcagggt cattaacaga gataagagca aggctattct tgtcaaccat taccatctta
      961 accttgcgtt cttcatcggt aatcacatag ttaatagtaa tttctgcaga agttcctgcc
     1021 gttgtattga tagccacaac tggcaagcct tttttagcag acttatgaag tccttcataa
     1081 tcctgtggtt ttccaccatt tgtagctatg atagaaatac aactagctgc atcctgtgga
     1141 gaacccccac caagactgat gataaaatca catccatgtt cttccaaagc agctacccca
     1201 tctgtgacat tcttacaagt agggtttggc tctacatcgc taaagatgac atattcgatt
     1261 ccttctatat ctagcggttt taaaacctta ggcaaaatgt cacttccttc gataaactta
     1321 tctgtcacca aaagtgcctt tttatagcca agttccttga tataaggacc cacttcattt
     1381 acaacacctt ttccaataag gttgactgcc ggaacataaa atgtagccat ataattttcc
     1441 tcctacgcct tcgcgctttt tctattatta tttttaaaat ctatgccata catttgtgaa
     1501 ataattaaca agtgtttttt aaaagtatag gagggacgta agctcacctc ctatacaaac
     1561 aagttttact tatgtagtgg tcccaacaac tgacatgcac gatagtccgc tgattctaaa
     1621 tcttctgttc caaataaaga ccaattttta ggtctaaaga tatcttcctc aggtacatta
     1681 tgcatattga caggaattct caacatagaa gccaaagtaa tcaagtctgc accaatgtgt
     1741 ccatatgtta tcgctccgtg attagctccc caattattca tgacatcata tacagactta
     1801 aaagcacctt ttcctgtcaa acgtggagca aaccaagtag ttggccatcc tggatctgtc
     1861 cgattatcta aagtatggtg aacatcttca ggaagttcaa gtgtgtaacc ttctgcaatt
     1921 tgtagcactg gaccaactcc ttttaaaaga ttgagacgta ccattgttac tggcatatcc
     1981 cccttcgtca agaagcgagt tgagaatcct cctccacgga agtattcgcg gtttgctggt
     2041 gggaagtctg tgttatcaag catagcctgc acttcacttt cttccaactc ccagaatggt
     2101 ttaataacag gttttccatc acgactagct tgcccagtac catctaatgt acaagagcca
     2161 gagttaatca aatgtaagaa gccatctgcg gcattacctt ctagagtatg tcctgttaca
     2221 cgtttaacag cctctggact ccaataagta cgcacgtcag caaatatttg tggggtattc
     2281 gtcaataagt aattaaagag catagacaca ccatttagtg aatcattctc tgttgcaaat
     2341 acaaatggtt tacgaatacc attccagtca aactgagtat tgaggaaagt ttccataaaa
     2401 tctccatttg ggaaatggtc tgtccactgg cgttgacctt gaaaaccagc tactaaagca
     2461 tggtgaccaa ctgcttcttc ctcaaaacca agttctgcta aacgaggatt cccctgcata
     2521 agatctcgac caatcatgaa catttttacc acaaattccc attgtttttc tttctcttct
     2581 ttagagagaa tcaaatcttc acgatttcgg tcaacacctt ctttaatatg ttcttttacc
     2641 caaaccatag cacgttcgaa ctcttctggg tcataaatac cacggtcaat acgacgtgta
     2701 aattccgtca tatctacaga ctcatttctc attcctaggt attcttggaa gaaatctgga
     2761 ttcacaatag aaccaccaat ccccattgat acactaccca ttgataggta agcagtatct
     2821 cgcatcaaac cagttgcaag agctgcacgg gcataacgta aaagtttttc tttgacatct
     2881 tctggaatat ctgtatcatt agcttcctga acatctcttc cataaatccc aaacgctgga
     2941 ataccttttt gagcatgtga agctagaaca gctgcaagat agacagctcc tggacgttct
     3001 gtcccattaa atccccaaat agcatgagga atgtctggag acatatccat agtttcacta
     3061 ccataacacc agcatggtgt gactgtaatt gttgcacaaa catttgattt tttgaacaac
     3121 tcatgtgaag ctgtagcctc tggaacacga ccaatggtag atggagaaat cacgcattcc
     3181 acaggttctc catctggata tttcaatgta cttgaaataa gatctgccat acttttagcc
     3241 atgttcattg tctgtacttc aagtgattct cgtacacctt gacgacgacc atcaatagtc
     3301 ggacgaatac caatacgtgg atgttgtatc ataatacttt cctcttgttt tatatattat
     3361 ttacccgtgg tgc
//
LOCUS       DATUCG010000184         3349 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.187, whole genome shotgun sequence.
ACCESSION   DATUCG010000184 DATUCG010000000
VERSION     DATUCG010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.187"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(17..649)
                     /locus_tag="VCA20_002298"
     CDS             complement(17..649)
                     /locus_tag="VCA20_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEP1836022.1"
                     /translation="MKEFKTIREDGIVEEEIKKSRFICFMKRVTSEEEARDFINKIKK
                     EHYKATHNCSAFVLGENMETKRSSDDGEPSGTAGVPMLTVLENHELTNVATVVTRYFG
                     GIKLGAGGLIRAYAGAVANAVKEIGVVEVKEQEGISISMSYAQYQDFANWRAEQGLEE
                     FDTQFTTEVSTMIFVDKEFLEQTLASLTDFYHGKVSVEKANSRIVEVPVY"
     gene            706..1998
                     /locus_tag="VCA20_002299"
     CDS             706..1998
                     /locus_tag="VCA20_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEP1836023.1"
                     /translation="MKELENYYGRLFTKYQLTAKEREIAEKVPSITKKNNCFRCGTTF
                     EEENKLPNDAYYCRACLLLGRVRSDEKLYHFPQKDFPITKCLKWKGQLTDWQQRISDG
                     LVANVENNRATLVHAVTGAGKTEMIYHTVASVIDKGGAVCLASPRIDVCIELYKRLQN
                     DFSVPISLLHGESEPYFRTPLVVATTHQLLKFYQAFDLVLIDEVDAFPYADNPILYRA
                     ADNAVKEAGVQVFLTATSTDELDKKVRTGKLSRLSLPRRFHGNPLVVPQKVWFSKFDD
                     TLKQNRLVPKLKKAIEEQRKSCFPLLIFVPEISKGQEFTKIMKKTFPEETIGFVSSQT
                     ENRLEIVEGFRKREITVLISTTILERGVTFPCVDVFVVQANHYLYTASSLIQIAGRVG
                     RSMERPTGLLQFYHEGSTGAIEKAIAEVKQMNKEAGYV"
     gene            1991..2656
                     /locus_tag="VCA20_002300"
     CDS             1991..2656
                     /locus_tag="VCA20_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEP1836024.1"
                     /translation="MSNCLLCGQAMKNKTRFSDLIFFSKEKSGICEECFSTFEEIAEQ
                     HCPHCYKNGESESCKDCQYWQNQGKPVVHTALYQYNQAMAHYFSRYKFQGDYVLRNIF
                     AKKLRIALSQFPDYTIVPIPISQKRLSERGFNQVEGLLNAANIPYQSLLGKYESQKQS
                     SKNRAERLEAKQMFYLLDEKEVPEKILLFDDIYTTGATIQLAVELFMKIGRKEIKTFS
                     LTR"
     gene            2733..3275
                     /gene="raiA"
                     /locus_tag="VCA20_002301"
     CDS             2733..3275
                     /gene="raiA"
                     /locus_tag="VCA20_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEP1836025.1"
                     /translation="MIKFSIRGENLEVTEALRSYVEEKVAKIEKYFNEEQELNAKVNL
                     KVYRDKRSKVEVTIPVGAVTLRAEDISQEMYGSIDLVVDKIERQIRRNKTKIERKHRQ
                     KVATGQVFTDELVEQTGEEVKVVRTKQVDLKPMDMEEAVLQLELLGHDFFIYTDANDG
                     TTNVLYRREDGDLGLLEVRQ"
BASE COUNT         1132 a          618 c          666 g          933 t
ORIGIN      
        1 tcgccgatag ctttttttaa tagacaggta cctcaacgat tcgagagtta gctttttcca
       61 cagatacttt tccgtggtaa aagtcagtga gacttgctaa ggtttgttca aggaattctt
      121 tatctacaaa aatcatggtt gaaacttcag tagtaaactg ggtatcaaat tcttccagac
      181 cttgctcagc tcgccaattg gcaaagtctt ggtattgggc ataggacatg ctaatggaaa
      241 tcccttcctg ctccttaact tccacaacac cgatttcctt gactgcatta gccacggctc
      301 cagcgtaagc acgaataagt ccgcctgccc cgagtttgat acctccaaaa tagcgggtaa
      361 ccaccgttgc gacattggtc agttcgtgat tttctaaaac cgtcagcatt ggcacaccag
      421 cagtaccaga cggctcccca tcatctgagg aacgcttggt ttccatattt tctccaagga
      481 caaaagcaga gcagttatga gtagccttgt aatgttcttt cttgattttg ttgataaagt
      541 cgcgagcttc ctcctcgcta gtcactcgtt tcataaagca gataaatcgt gactttttaa
      601 tttcttcttc aacaatacca tcttctctaa ttgttttaaa ttctttcata accaaattgt
      661 actaattttt ttaaaaactg acaagaaatt ccgaataaat aagcgatgaa agaattagaa
      721 aattattatg gaagattatt taccaaatat caattgacag caaaagaaag agaaatagca
      781 gaaaaagtgc caagtattac aaaaaagaat aactgctttc gctgtggaac aacttttgaa
      841 gaagaaaaca aattgccaaa cgatgcttat tactgtcgag cctgcttgct tctaggcaga
      901 gtacggtcag acgaaaaact ctatcatttt cctcagaaag attttccaat cactaagtgt
      961 ttaaagtgga aaggtcaact aactgattgg caacaaagaa tttcagatgg actagttgca
     1021 aacgtggaaa ataatcgtgc gacattggtt catgcagtaa caggagcagg taagacagaa
     1081 atgatctacc acaccgttgc ctcagtgatt gataaaggcg gagcggtttg cctagccagt
     1141 cctcgaattg atgtttgtat cgaactctat aaacgtctgc aaaatgactt ttcagttcca
     1201 attagtttac tgcatggaga gtctgaaccc tatttccgaa ccccattagt tgtagcaacc
     1261 acacatcagt tattaaaatt ttatcaggcc tttgatttgg ttttgattga tgaagtagac
     1321 gcctttccct atgcagataa tcccatactc tatcgagcag cagacaatgc ggtcaaggaa
     1381 gctggtgttc aagtttttct gacagcgact tcaacagatg aattggataa aaaagtcaga
     1441 acaggtaaat taagtcgtct tagtttgcca aggcgctttc atggcaaccc acttgttgtc
     1501 ccgcaaaaag tctggtttag taaattcgat gataccctaa agcaaaatag actagttcca
     1561 aagttgaaaa aagcgattga agaacagaga aagtcgtgct ttcccttact catttttgtc
     1621 ccagaaatct ccaaaggtca agaatttacc aagataatga aaaaaacatt cccagaagaa
     1681 acaattggct ttgtatccag tcaaacagaa aatcgccttg aaatagttga agggtttcgc
     1741 aagagagaaa tcacagtctt aatctcgacg actattcttg aacgtggggt aaccttccca
     1801 tgtgtagacg tctttgttgt tcaagccaat cattacctct acacagcgtc aagtcttatt
     1861 cagattgcag gccgggtcgg caggagtatg gaacgtccga ctggtttact ccagttttat
     1921 catgagggaa gtacaggagc cattgaaaag gcaatcgctg aagttaaaca gatgaacaag
     1981 gaggctggtt atgtctaatt gtctattgtg cggtcaagct atgaaaaata aaacaagatt
     2041 ttcagatctc atcttcttta gtaaagaaaa atcgggcatc tgtgaagaat gtttttcgac
     2101 ttttgaggaa atagcagagc aacactgtcc acattgttat aaaaatgggg aatcggaaag
     2161 ttgcaaggat tgtcagtact ggcagaatca agggaaacca gttgtccaca cagctttgta
     2221 ccaatataat caagcgatgg cacattattt tagtcgctac aaatttcaag gagactatgt
     2281 tttaagaaat atttttgcta aaaagcttag aatagcgtta agtcaatttc ctgactacac
     2341 aattgttccc ataccaatta gtcaaaaacg actatcagaa cgcggtttta atcaagtgga
     2401 aggacttttg aatgctgcaa atattcccta tcagtccctt cttggaaaat atgaaagcca
     2461 aaaacaatca tctaaaaatc gtgcagagag actagaagca aaacagatgt tctatctgtt
     2521 agatgaaaaa gaagtgccag aaaaaatatt attattcgac gatatatata caacaggagc
     2581 cacaatccaa cttgctgtag aacttttcat gaaaattggt aggaaagaaa tcaaaacatt
     2641 ttcactaaca cgataagaaa acggttgcaa tattcctata atgtggtata ataatagtga
     2701 agaaaggcga aagccaagaa agaggtacta atatgattaa attcagtatt cgtggagaaa
     2761 accttgaagt tacagaagca cttcgcagct atgtagaaga gaaagtagct aagattgaga
     2821 aatatttcaa tgaagaacaa gagttgaatg ctaaagtaaa tctaaaagtt taccgtgaca
     2881 aacgctcaaa agtagaagtg acaattccag ttggagcagt tacacttcgc gcagaagata
     2941 tttcacaaga aatgtatggc tctatcgatc ttgtggtaga taagattgaa cgccaaattc
     3001 gtcgtaacaa aaccaagatt gaacgaaaac atcgtcaaaa agtggcaact ggtcaagtct
     3061 ttacagatga acttgttgag caaacaggtg aggaagtaaa agtggttcgt actaagcaag
     3121 tagacttgaa accaatggat atggaagaag cagtcctcca attggagttg ctcggacatg
     3181 atttctttat ctatacagat gctaatgacg gtacaacaaa tgtattgtat agacgcgaag
     3241 atggagattt gggtcttcta gaggtacgtc aataaagata ataaaacagt tgcagttttt
     3301 gtagctgttt tattattttc aaaaaaccgg ttgacaatgt atataagtc
//
LOCUS       DATUCG010000185         3295 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.188, whole genome shotgun sequence.
ACCESSION   DATUCG010000185 DATUCG010000000
VERSION     DATUCG010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.188"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            96..833
                     /locus_tag="VCA20_002302"
     CDS             96..833
                     /locus_tag="VCA20_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEP1836026.1"
                     /translation="MLNIFVLEDDFFQQSRLENAIRQCVEETSVRYKFLEVFGKPNQL
                     LESIEEAGNHQFFFLDIEIKGEEKKGMEIAKEIRARDPYAVIVFVTTHSEFMPVTYRY
                     RVSALDFIDKSLEDSDFQKAVSDVLVHAFENIDHTIAENSFIYKTETAHIQVPFSDIL
                     YFETSSTIHKVILKTKTGQTEFYGKVSDIAKADERLYQAHRSCVVNPLNITRLDRTNH
                     IAYFENGDSCFVSRLKQKKLADLLGSD"
     gene            891..2168
                     /locus_tag="VCA20_002303"
     CDS             891..2168
                     /locus_tag="VCA20_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEP1836027.1"
                     /translation="MIFEQISGLKLKNITKLNISFLLTVIQILFFSVYLLIEPISLLV
                     VLQFFKTKWNKTQKLFYGLLPIVIGDMFQRIISLYLRFIFNVDIEYANNSLLFDILLP
                     VLLVPFYYGFFKGLRVEEKYLKVDTIDRDFRTIFWGLNTLFISYLVLIRGNLFLEILI
                     EGRVLNISPDTYFIRTNILIIYFILFTVSMLYLNYQKKEKQENEIQELKDKQLADLGR
                     YSRHVESLYKEIRSFRHDYTNILISLNEAIKEEDIVAIRTIYSEVIADSDRKFYDGKY
                     AIARLSNIQNPAVKSLLSSKMLEAQKKGISISVEVDAEIEPPALESIEFITILSILLD
                     NAIDAAEQCTDGNIVFAYFQEDDRKIVVVENTTVEDKVVTSHIYEYGHSTKGDNRGIG
                     LANVKTILDNYPKFYISTNSSNHRFVQELVFLE"
     gene            2595..2762
                     /locus_tag="VCA20_002304"
     CDS             2595..2762
                     /locus_tag="VCA20_002304"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1836028.1"
                     /translation="MIWLIKLLLIVVISIPVTSILQPFQNHYLNGVTVAVISVIVSHL
                     VDKYGKGGDEV"
     gene            2759..2947
                     /locus_tag="VCA20_002305"
     CDS             2759..2947
                     /locus_tag="VCA20_002305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1836029.1"
                     /translation="MSEELKRMLKAGTLFLALFVLNVFFLKWLSVIGFVIHFSEISYL
                     VPPLFSVIVLSMIEKREV"
BASE COUNT         1112 a          408 c          631 g         1144 t
ORIGIN      
        1 catcggcagg tctatcaagt caagttggac atttatcttt tgctgaaata tgctataata
       61 atgattatta cttacaaaaa atgtaggaga tttctatgct taatattttt gtattagaag
      121 atgatttttt tcagcagagt aggctagaaa atgctattag gcagtgtgtt gaagaaacgt
      181 cagtaagata taaattccta gaagtttttg gtaaaccaaa tcagttattg gaatcaattg
      241 aggaagcagg gaatcaccaa tttttctttt tagatattga aattaaaggc gaagaaaaga
      301 aaggaatgga aatcgctaaa gaaatccgtg ctcgagatcc ttatgccgtt attgtctttg
      361 taacaactca ctcagaattt atgccggtaa cgtatcgtta tcgggtttct gctttagatt
      421 ttatagataa aagcctagag gatagtgact ttcaaaaggc agtatcagat gtgttagtgc
      481 atgcttttga aaatattgat catactatag ctgaaaattc ttttatatac aaaactgaaa
      541 cagctcatat tcaagtccct tttagtgata tcctttattt tgaaacatca tcaacaatac
      601 ataaagtcat tttaaaaacc aaaacaggtc agactgagtt ttacgggaaa gtatccgata
      661 tagcgaaagc tgacgaacga ctttaccagg cacatcgttc ttgtgtagtg aatccattaa
      721 atataacgag attggacaga acaaaccata ttgcctattt tgagaatgga gattcttgtt
      781 ttgtttctcg tttgaagcag aagaaacttg cagatttgtt ggggagtgat taatgagtat
      841 tttttctcta ttgaagacat tctttttttg ttggcgtatt aattagattg atgatttttg
      901 agcaaattag tggtctaaaa ttaaaaaaca taacaaaatt aaatatatca tttctattga
      961 ctgtaataca gatacttttt ttcagtgtat atttgttaat tgaaccaata tcattattag
     1021 tagttcttca attttttaaa acaaaatgga ataaaactca aaaactattt tatgggttac
     1081 ttcctattgt tattggggat atgtttcaaa ggataattag tttatattta cgttttatat
     1141 ttaatgtaga tatagagtat gccaataata gtctattatt cgatatatta ttacctgtat
     1201 tgctagttcc cttttattat ggttttttta aaggtttaag agttgaagaa aaatatctga
     1261 aagttgatac aatagatagg gattttagaa caatattttg gggattaaat actctgttta
     1321 taagttattt agtactaata agggggaatc tttttttgga aatcctaata gagggaaggg
     1381 tattaaatat ttctcccgat acttatttta ttagaacaaa tatattgata atttatttta
     1441 ttttatttac tgtgagtatg ttgtatttaa actatcagaa gaaagagaag caagaaaatg
     1501 aaattcagga gttaaaggac aaacaattag ctgatttagg ccgttatagt cgtcatgttg
     1561 agtcacttta taaagaaatt cgaagtttcc gacatgacta tactaatatt cttattagtc
     1621 tgaatgaagc aattaaggaa gaggatattg ttgcgattag aacgatttat agtgaggtta
     1681 ttgcagattc tgatagaaaa ttctatgatg gaaagtacgc tatagctaga ctatccaata
     1741 tacaaaatcc tgctgtgaag agtttgctgt catctaagat gttggaggcg caaaaaaaag
     1801 gtatttcaat ttctgtggaa gtagatgctg aaattgaacc acctgcttta gagtcgatag
     1861 aattcataac gattctgtct attttattag ataatgctat cgatgctgca gagcagtgta
     1921 cagatggaaa tattgtattc gcctatttcc aggaagatga ccgaaaaatt gtggttgtag
     1981 aaaatactac tgttgaagat aaggtagtaa caagtcatat atatgagtat ggtcactcta
     2041 caaaaggaga taatcgagga attggcttgg ctaatgtaaa aactatttta gataactatc
     2101 ctaaatttta tatatcaacg aacagtagca atcatcgatt tgttcaggag ttggtgtttt
     2161 tggaataaga tttatagatg taagtccagt agaaaggctg gatttttttg catcttctcg
     2221 aaaaaatggt cattcattac aaaataacga cagttcatga catggatgaa aaaatatggc
     2281 tgatttggca tatactaaga gtgtaaaaaa taagaacaag gaaactattt taaaaggagg
     2341 gcgttaacat gcaaagcgag aatagaaaca ctaaatatgt gatgaacgta ataaaaaata
     2401 caatctacct atctatgttt tcagtacttg ttttggattt gattatggaa ggtaaaataa
     2461 attttgaata cattttaagc tggggattat tgttatattt cattgatgac tgatagtttc
     2521 acatgttttg acagtaaata gtaaatgcaa ttttggttgc tatctattca gtatatctta
     2581 ggaggatttg ttttatgatt tggcttatta aacttctttt gattgttgtg atttctattc
     2641 ctgtaacaag tattttacag ccttttcaaa atcattactt aaatggagtc acggttgcgg
     2701 tcatttccgt cattgtgtcc catttagtgg ataaatatgg gaaaggtggg gatgaagtat
     2761 gagtgaagag ctgaaaagga tgttgaaagc tgggacactt tttcttgcct tatttgtctt
     2821 aaatgtgttt tttcttaaat ggttgtctgt tataggattt gttattcatt ttagtgaaat
     2881 tagttatttg gttccaccgc tgttttcagt catcgttcta tcgatgattg aaaaaagaga
     2941 agtatgaaaa tgacttaatg aaaactggtc attcattaca aaataacgac cgttcatgac
     3001 atggatgaaa aatttggtct gagttggcat atactgaatg tgtaaagaaa caaggaaaat
     3061 aagaacaagt ctagtggtaa aaagtcaata gaaagttgat taatttttag ttgatttttt
     3121 agaaaacagg gtgcactaaa aacacctagc gaaaaacgct ttttttcact agcttaatca
     3181 aggacttgtg ccgataatca cttatcttcc ataagcttcc ttggtggcgt atagagttgg
     3241 ggattgcgta gtagcgcatc caccagacgc acaaattttc tagcggttaa gacga
//
LOCUS       DATUCG010000186         3218 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.189, whole genome shotgun sequence.
ACCESSION   DATUCG010000186 DATUCG010000000
VERSION     DATUCG010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.189"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            32..3097
                     /locus_tag="VCA20_002306"
     CDS             32..3097
                     /locus_tag="VCA20_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002266544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEP1836030.1"
                     /translation="MPNVDYTKISLPVAELTNGIVLEEFIKSERYNRDIYQTESEMEK
                     RLIDDLVQGQQYERFLGHSTTELLANLKLQLENLNGVSFSAMEWQRFLDEYLNKPNEG
                     MVEKTRKIQEDHIYDFVFDDGHVQNISLLDKKNIHRNKLQVMNQMIQKGSHTNRYDVT
                     ILVNGLPLVQIELKKRGVPISQAFHQIHRYTKESFNSENSLFQYIQLFVISNGTFTRY
                     FSNTVERNKNNFEFTCEWADRKNHAIHDLEDFAQTFLNKTTLLSILIKYCIFNAEDVL
                     LVMRPYQIAATESILNRIHFAYQNKLYGKVEGGGYIWHTTGSGKTLTSFKTARLATEL
                     EYIDKVFFVVDRKDLDYQTMKEYQKFQKDSVNGSNDTKQLKEAIEKDDSRIVVTTIQK
                     LHHFTKKYPNHPIYDKQCVMIYDECHRSQFGDSQKKVNSSFRANYQFGFTGTPIFADN
                     TLTGQTTQDIFGHQLHSYIITDAIRDNKVLKFKVDYNNVAPKFREEEVQAGHEKDLRI
                     IRQIEKKALLAPERISKVVEHILSVYPVKTHREISHIVKIKNVDGDTLRKQRMRGYNA
                     MLAVQNVYAAKMYYEELHNQQASIPEECRLRIGMIYSFAANEEQVAYGDIYDEDFNPS
                     AMESSAKEFLDRVIADYNKSFSTSFSTESQSFQNYYKDLSQRTKNKEIDLLIVVGMFL
                     TGFDAPTMNTLFIDKNLRYHGLIQAYSRTNRILSKDKPFGNIVCFRDLEKATIDALKL
                     FGEDNNIYITLEESYQEYMEGFEDEETGVIVRGYKEVCREMLTKFPDPTEFYLESEKK
                     EFVGLFGELLKLDNVLRNFDEFTDEERLISQGLMQDYHSKYVDIREESYHSGGRDGHQ
                     PEIDLSDLEFEIELLKSDEINLDYIIALISEKANEDITKEQLQNQVSRVIRSTIDMRA
                     KERLVMDFIRDSNLDELKDNQTILEAFYHYARKSKQEQIELFAQTQQLQGDYKLFIEA
                     AILRGFASTAGTDLNALMPPTSRRQGAREKKKQEILHQLQELVETFTGI"
BASE COUNT         1082 a          502 c          669 g          965 t
ORIGIN      
        1 actatagtat tattttaacc gagagatgaa gatgccaaat gtagattata caaaaatcag
       61 cttacctgtt gcggagttga ctaacggaat tgttctagaa gagtttataa aatcagaacg
      121 ttacaatcgc gatatttatc agactgaaag cgagatggag aaacgcctaa tagatgattt
      181 ggtgcaggga cagcagtatg aacgtttttt aggacatagt actacagaat tacttgccaa
      241 tctaaaacta cagttggaaa atttgaatgg tgtgagtttt tcagcaatgg agtggcaacg
      301 tttcttggat gaatacctca ataagccaaa tgaaggaatg gttgaaaaaa ccagaaaaat
      361 tcaagaagac catatatatg attttgtttt tgacgatgga catgtgcaaa atatttcact
      421 acttgataag aagaatattc atcgaaataa actgcaagtg atgaaccaga tgatccagaa
      481 aggtagtcat accaatcgct atgatgtgac tattttggtc aatggtttgc ctttagtaca
      541 gattgagttg aaaaagagag gtgttcccat cagccaggcc tttcatcaga ttcatcgcta
      601 taccaaggaa agtttcaata gtgaaaattc tctttttcaa tacatacagc tgtttgttat
      661 ttcaaatggg acatttacac gctatttctc aaatactgtt gagcgaaaca agaataattt
      721 tgagtttacc tgtgagtggg cagatagaaa aaatcatgct attcatgatt tagaggattt
      781 cgctcaaaca tttttaaata agacaacgct tctatcgatt ttgatcaagt actgtatttt
      841 taatgctgag gatgttctcc tagtgatgcg cccatatcag attgcggcga cagaatccat
      901 tttaaatcgt attcattttg cttatcaaaa caagctctat gggaaagtag aaggaggagg
      961 ctacatctgg catacaacag gatcaggtaa gactctgacg tcttttaaga cagctcgatt
     1021 ggctacagaa ttggagtata ttgataaggt tttctttgtg gtggaccgca aggatttgga
     1081 ctatcaaacc atgaaggaat atcaaaaatt ccagaaagat tctgttaatg gtagcaatga
     1141 taccaagcaa ctaaaagagg caattgagaa agatgatagt cgtattgttg tcacaacgat
     1201 tcaaaagctg catcatttca ctaaaaaata ccctaatcat ccaatttatg ataagcaatg
     1261 tgtcatgatt tatgacgaat gccatcgttc gcagtttggg gattctcaga agaaagtaaa
     1321 cagtagtttc cgagccaatt accagtttgg ttttacagga acaccgattt ttgctgataa
     1381 tactctcact ggtcaaacaa ctcaggacat atttgggcat cagttgcatt cctatattat
     1441 tacagatgcc attcgtgata ataaggtttt aaagtttaag gtagactata acaacgttgc
     1501 tccgaagttt cgtgaggaag aggtccaagc aggtcacgaa aaagatctcc gcattattcg
     1561 tcaaattgag aaaaaagccc tgttggctcc tgaacgcatt tctaaggtag ttgaacatat
     1621 cctatccgtc tatccggtta aaactcatcg tgagattagc catattgtta agataaaaaa
     1681 tgttgatgga gatacgttaa gaaagcaacg catgcgaggt tacaatgcga tgttggctgt
     1741 acaaaatgta tatgctgcta agatgtacta cgaagagtta cataatcagc aggcaagcat
     1801 tccagaggaa tgtcgtttga gaattgggat gatatacagc tttgctgcaa atgaggagca
     1861 ggttgcctat ggtgatattt atgatgaaga tttcaatccc tcagcgatgg aatcatccgc
     1921 gaaagaattt ctagataggg tgattgctga ttataataag tcttttagta cttcattctc
     1981 aacagaaagt caatcctttc agaactatta taaagacttg tctcaacgaa ccaaaaataa
     2041 ggaaattgat cttctgattg ttgttggaat gtttttgaca ggttttgatg cccctacaat
     2101 gaataccttg tttatagata aaaatttacg ctaccacggt ctgattcagg cttattcacg
     2161 gaccaatcgt attttgtcaa aggacaagcc gtttggaaat attgtttgct tccgagattt
     2221 ggaaaaagct acaattgatg ctttaaagtt attcggtgag gataacaata tctatattac
     2281 gctggaggaa tcctatcagg agtatatgga aggttttgag gatgaggaaa caggtgttat
     2341 agttcgtggg tataaagaag tttgtcgaga aatgttgaca aagtttccgg atccgactga
     2401 attttatctg gaatctgaga aaaaagaatt tgttggttta tttggtgaat tactgaaact
     2461 cgataatgta ttacgaaatt ttgatgagtt tacagatgaa gaacgcttga tttcacaggg
     2521 acttatgcaa gattatcata gtaagtatgt cgatattcgt gaggaatcct accactcagg
     2581 gggacgtgat ggtcaccaac ctgagattga tctgtccgat ttagaatttg aaattgagtt
     2641 gttaaaatca gatgagatca acctcgacta tatcatcgcc ttaatttctg aaaaagcaaa
     2701 tgaagatatt acaaaagaac agcttcaaaa ccaagtatca cgtgtgattc gttcaactat
     2761 tgatatgcgt gcaaaagaaa ggcttgttat ggatttcatt cgtgatagta atctagatga
     2821 gttaaaagat aatcagacta tattagaagc tttttatcat tatgctagaa agtcaaaaca
     2881 ggaacagatt gaattattcg cacaaacaca acaattgcaa ggcgattata aactatttat
     2941 tgaagcagct attttgcgag gttttgcatc aactgctgga acagatttaa atgcgcttat
     3001 gccaccaact tcaagacggc aaggtgcgcg tgagaaaaag aaacaagaaa ttttacacca
     3061 gttacaagaa ttggtcgaaa catttacagg aatctaaagg agtaagtttg aaaaaataaa
     3121 agctaattga tttatagttt atcctaattt tgggcgcact aaaaagaggt gttgaaacct
     3181 gatttttgag attaagcgac attgaatccg tgttgttg
//
LOCUS       DATUCG010000187         3147 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.190, whole genome shotgun sequence.
ACCESSION   DATUCG010000187 DATUCG010000000
VERSION     DATUCG010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.190"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(<1..613)
                     /locus_tag="VCA20_002307"
     CDS             complement(<1..613)
                     /locus_tag="VCA20_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEP1836031.1"
                     /translation="MKNLPKIELNNDEKLFLFEIDSRIKALLLELPESYHNSLQIVRG
                     SRFRPLLTYYGYTLYSDELNETIYQSATAIELIHKASIIIDDIIDQDKKRHGMLTVHQ
                     QYSLNEALILSVFLLGKCIEMLSDIDDSTVRLFSQMIIKMCQGTLSELNINDETAIAD
                     IRKILDNQTSQVIQNSLLIGMKSYNESINNQYLEIVGYKLGYLF"
     gene            704..1591
                     /locus_tag="VCA20_002308"
     CDS             704..1591
                     /locus_tag="VCA20_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000384879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEP1836032.1"
                     /translation="MDYTIISNYLDYCKAHKRLSTHTLRAYKNDLMQFYNSNYECVVS
                     YIEGLTNSNLKTSTLRRKIASLKVFYGYLQLKNVITDNPFNQLRFQFRAEKTLPKTIP
                     HDSLKILYTYLQKKIISAKTIYQKQKAERNLLIVSLLLSTGIRISELCHIQIKDINLT
                     EQTLQILGKGKKERMIFIGDERTFQLLKAYITCYCNHTDDYLFPGKSLKKPLTEQSVR
                     LLLKTAKIQTRLTTTITPHMFRHSFATMLLDNDVDIRYIQQILGHSSISITQIYTHVS
                     QSRQKEILSSYNPISSIYT"
     gene            complement(1637..1936)
                     /locus_tag="VCA20_002309"
     CDS             complement(1637..1936)
                     /locus_tag="VCA20_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1836033.1"
                     /translation="MLEQILQSLLIIAAIGLMLFVLYRIVKVSAALFLIGLISGLVFI
                     EIYGIYLFFTERYLYSEDLVANGIWSFTGFFIIFNILLVLGLMTNLVKSRITEYK"
     gene            complement(1950..2249)
                     /locus_tag="VCA20_002310"
     CDS             complement(1950..2249)
                     /locus_tag="VCA20_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000194854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1836034.1"
                     /translation="MGQTVEEMRYQLEEWLSQGFTSSEDRANYQTLKEQYEDETFDYS
                     FSRREITGQLELIITSRENDFPSLDEVTKTEYLDLVAQLDDLDKRQADYYRKQLA"
     gene            complement(2320..>3147)
                     /locus_tag="VCA20_002311"
     CDS             complement(2320..>3147)
                     /locus_tag="VCA20_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase SNF2"
                     /protein_id="HEP1836035.1"
                     /translation="KYITQIMTSKDPVRSAEDIDEQTMTASDFKALATGNPYLKLKME
                     LENELTVLENQKRAFNRSKDEYRHTISYSEKHLPIMEKRLSQYDKDIAQSLATKSQDF
                     VMRFDNQVMDNRAEAGDYLRKLITYNRSETKEVKTLASFRGFDLKMTTRAPSEPMPET
                     VSLMIVGDNQYTVALDLKSDVGTIQRISNAIDHIIDDQEKTQELVKDLKDKLRVAKVE
                     VEKVFPKEEDYQLVKAKYDVLAPLVEKEAEIEEIDAALAKFSEDTTPQKKQQIALEI"
BASE COUNT          997 a          617 c          471 g         1062 t
ORIGIN      
        1 gaaacaaata ccctaattta taacctacta tttccaaata ttgattatta atagattcat
       61 tataggattt cataccaatc aataaactat tctgtattac ttgagaagtt tggttgtcca
      121 aaatttttct aatatctgca atagctgtct catcgttaat atttaattca ctcaatgttc
      181 cttggcacat cttaataatc atctgactaa ataatctaac agtagaatca tcaatatctg
      241 acagcatttc tatacacttt cctagtaaaa atactgataa tatcaaagct tcatttagag
      301 aatattgttg atgaacagtt agcataccat gccttttttt atcttgatca ataatatcgt
      361 caataataat agaagcctta tgaattaatt cgattgctgt ggctgattga taaatagttt
      421 catttaactc atcagaatat aatgtatacc cataatatgt taataggggg cgaaaacgac
      481 ttcctcttac aatttgtaag ctgttatgat atgattcagg caactctagt aataatgctt
      541 ttattctaga atcaatttca aataaaaaga gtttttcatc attatttaat tcaattttcg
      601 gcaaattttt catagttctc tcctaccgat aacaatttat tatcactata caaagcacca
      661 aaatcagtct ataatagcgt cattatcgtt aaaggagact atcatggatt atactattat
      721 ttctaactat ttggattatt gtaaagctca taaacgcttg agtacacaca ccttgcgggc
      781 ttataaaaac gatttaatgc aattttacaa ttccaattac gaatgtgtgg taagttatat
      841 tgaaggttta acaaattcaa acttaaagac tagtacttta cggcgaaaaa tcgcaagttt
      901 gaaagtgttc tatggctatc ttcaattgaa aaatgttatt accgacaatc cctttaatca
      961 attacggttt caatttcgag cagaaaaaac attgccgaag acaattcctc acgatagttt
     1021 aaaaatccta tatacctatc tacaaaagaa gattatctca gccaaaacta tttatcaaaa
     1081 acagaaagct gaaagaaatt tgttaattgt ctccctatta ctttctactg gcattcggat
     1141 ttctgagctg tgccatattc aaatcaagga tatcaatctc actgaacaaa cactccaaat
     1201 tctaggaaaa ggtaaaaaag agcgtatgat ttttattggg gatgaacgaa cttttcagtt
     1261 attaaaagct tatatcactt gttattgcaa tcatacagac gattatttat ttcccggaaa
     1321 atccctaaaa aaaccactga cagaacaaag tgttcgtttg cttctaaaaa cagccaaaat
     1381 acaaaccaga ctcactacaa ctattacacc tcatatgttt cgccacagct ttgctacgat
     1441 gttattagat aacgatgtcg atattcggta catccagcag atacttggac acagttctat
     1501 ttcgattact caaatatata cacacgtatc acaatcaaga caaaaagaga tactaagttc
     1561 ctataatcca atttcatcta tctacactta atcaaaaaac agacaaaaat aaaagactac
     1621 ctagaatcag gtagccctat ttgtattccg taattctact tttcacgagg ttggtcataa
     1681 gtccaagcac tagcagaata ttaaaaataa taaaaaatcc tgtaaaactc caaataccat
     1741 tggcgactaa atcttctgaa tagagatatc tctcagtaaa aaataggtaa attccataga
     1801 tttctataaa gactaatcca ctgatgagtc cgataagaaa caaagcagct gaaactttca
     1861 caatccgata taggacaaac aacatcagtc ctattgctgc gataatcaaa aggctttgta
     1921 gaatttgctc caacatctta cacctctttc taggctaatt gcttgcgata gtagtcagcc
     1981 tgtctcttgt ctaaatcatc tagttgggca accaaatcaa ggtattccgt ctttgtcacc
     2041 tcatctaagc taggaaaatc attctcacga cttgtgatga tgagttccag ctgtccagta
     2101 atttcacgcc ttgaaaaact gtaatcaaag gtctcatctt catactgttc ctttaaagtt
     2161 tggtagtttg ccctatcttc tgaactcgta aagccttgtg acaaccattc ttctagttga
     2221 taacgcattt cttctactgt ttggcccatg atctattctc cttttgtgat ttcttgactt
     2281 cattttaccg cgttcaaaat tatttgtcag ccttttttct tatatctcga gtgctatttg
     2341 ttgcttcttt tggggtgttg tatcttcact aaacttggcc aaagctgcat ctatctcttc
     2401 aatctctgct tctttttcaa ccaagggagc taaaacatca tacttagcct ttacaagctg
     2461 atagtcctct tcctttggaa agactttttc aacttctact ttggctactc gtagcttatc
     2521 ttttaaatcc tttaccagct cttgcgtctt ttcttggtca tctataatat ggtcaatggc
     2581 attactaatc cgttgaatgg ttcccacgtc tgatttcaag tcaagggcga cagtatactg
     2641 attgtctcct acaatcatta aagagacagt ctctggcatg ggctcactag gagctcgtgt
     2701 tgtcattttc aaatcaaatc ctctaaagct ggcaagtgtt ttgacttcct tggtctctga
     2761 gcggttatag gtaatgagtt ttcgcagata gtccccagct tcagcacgat tatccattac
     2821 ttgattgtca aatcgcatga caaaatcttg cgactttgtt gccaaagatt gggcaatatc
     2881 cttatcatat tgactcaacc gtttttccat aatagggagg tgcttctcac tataggaaat
     2941 ggtatggcga tactcgtctt tggagcgatt aaaggctcgt ttttgatttt ctaaaactgt
     3001 cagttcattt tccaattcca ttttgagctt aaggtaggga ttaccagtgg ccaaagcctt
     3061 aaagtcagaa gccgtcatgg tttgttcatc aatgtcttca gctgatctca caggatcttt
     3121 tgaggtcatt atttgggtga tatactt
//
LOCUS       DATUCG010000188         3138 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.191, whole genome shotgun sequence.
ACCESSION   DATUCG010000188 DATUCG010000000
VERSION     DATUCG010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3138
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.191"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            <1..>3138
                     /locus_tag="VCA20_002312"
     CDS             <1..>3138
                     /locus_tag="VCA20_002312"
                     /inference="COORDINATES: protein motif:HMM:NF018196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEP1836036.1"
                     /translation="AVRSNVDALVLELRKYYPDGEITAVLNTAAGTPAATTIDVTNPA
                     SASYAQHGVWEIPDPVKAANSGLTTAEMKATITEVEKKYPAGYIGDQDANRDTFLVFT
                     LDTIDGYGNGEGFNDWVKHNYYLTFSVSTDAAERKAKDVIYAKLVEKTINGDKVIRTV
                     ELQPNVESKIDELTVLENDSTHKTVYKFTYLKSEQGTTVRQLSIENSNGKNMNVPLYN
                     KLDQSKVGMETNSYVTLVPGSTSQTTSYYVKENKETGRLRELLARYTQDKGLIGDKFQ
                     IGGIVDFDNYELIESELPDVESGILQSDYVVGTQWAVIRGAWNEARLYTITADDGTEQ
                     IQLYMLDPNNPDWAKFYKSNNVTIDDLAEYFRLMFTSENIKPGEVNTKSGTFSAKFYE
                     ADKLVLESTQVQVLDANGDVIGTLNDAKTAYTTPSGETVAVKSVTNTMVTDEDGTIHT
                     INNLKVYKTNADGDYVDANGAVLKPLQWRNDVPGVYKNDPKSKYYGKATFESDGVEVS
                     TGAFSRTVIDPVTGQKVTETFDNGNWMFGVNYKTDPSETRLDDAGQNKGKDTRWDLGG
                     LEMSMANGNAKNEQHARYWYTEKGGVDVYYITSDGKVLTDFTTSDGKTVADKVTIVGH
                     GNTDSAYDTTSVRYPTITAADGTVYYYKEIDTAAKNLHPVVDDKTDTDYRSNEKIDAE
                     KGTIKTDTVKQLTYVYEKAGNVNVHYVDTDGNELQSSVEDVTNGKPGTGYDTVLDNKQ
                     KEITVNGTVYYLVPAGEYKVGTVGDENNLTVVGNGKATGIDATTGTVDAGVTKNVTYV
                     YQKGGDVIVNYVDQDGNPISGTTDKGTTTESTVTDTSYTKAGTAYNTTDLKPTTITTT
                     DGKVYQLVPEATKGNEDGTVESGVTKEVTYVYKEVKGSVVVNYISTTGEEIQAQVVDT
                     PESSTGTEYDTTDVKPETITTKDGRTFKLVPKLTQGSEKGEVVPGVTKVTYVYEEVKG
                     DVVVNYVNTDGKVIATQVVDTKTTSTGTAYDTTDNKPEKIVEDATGDVYYYKEVQAGS
                     NETGKVVEGTTEVTYV"
BASE COUNT         1104 a          573 c          674 g          787 t
ORIGIN      
        1 tgcagttcgt tctaacgtag acgctttagt cttagaattg cgtaagtatt atccagatgg
       61 tgaaattact gcagtcttga ataccgctgc tggaactcca gctgcaacaa ctattgatgt
      121 tactaacccg gcatctgcct cttatgctca acatggtgtt tgggaaatcc cagatccagt
      181 taaggctgct aactcaggtt tgaccactgc tgagatgaaa gcaacaatta cagaagttga
      241 gaaaaaatac ccagctggct atataggaga ccaagatgct aaccgtgata ccttcttagt
      301 cttcacactt gatactattg atggatacgg taatggtgaa ggtttcaatg attgggttaa
      361 acataactac tacttaacat tctcggtttc aactgatgca gcagagcgta aagcaaaaga
      421 tgttatctat gctaaactag ttgaaaagac catcaatggc gacaaagtga ttcgaacagt
      481 tgaattacag ccaaacgtag aaagtaagat tgacgagttg actgttttag aaaacgatag
      541 cacacacaaa acagtttata aatttactta tctaaaatca gagcaaggta caaccgtccg
      601 tcaactttcc attgaaaaca gtaatggtaa gaacatgaac gttccactgt ataacaagct
      661 ggaccaatct aaggttggta tggagacgaa ctcttatgtt acgctggttc caggaagcac
      721 aagccaaaca accagctatt atgtcaaaga aaacaaagaa actggtcgcc ttcgtgagtt
      781 gctagcacgc tatactcaag acaaaggact tataggagat aagttccaaa tcggtggtat
      841 tgttgatttt gataactatg agttgattga gtcagagttg cctgatgttg agagcggcat
      901 tctacagtcc gactacgtag ttggtacgca gtgggcggta atccgtgggg cttggaacga
      961 agctcgtcta tataccatta ctgccgatga tggtacagag caaatacaat tgtacatgct
     1021 ggatccaaac aatccagact gggctaagtt ctacaaatca aataatgtca ctatagatga
     1081 cttggctgaa tatttccgtc tgatgttcac ctctgaaaac atcaagcctg gtgaagtaaa
     1141 taccaaatcg ggaacattta gtgctaagtt ctatgaagca gacaaactcg ttcttgaatc
     1201 aacacaagta caagtgctcg atgcaaacgg tgatgtcatc ggtacgctaa atgatgccaa
     1261 gacggcttat acaacccctt caggagaaac tgtagcagtt aaaagcgtaa cgaatactat
     1321 ggtaacagat gaagacggga ctatacacac aatcaacaac ttgaaagtct ataagacaaa
     1381 cgcagatgga gactatgttg atgcaaatgg agcagtcttg aaaccgttgc aatggcgtaa
     1441 cgatgtacca ggcgtctata aaaatgatcc aaaatcgaaa tattatggta aagcaacatt
     1501 tgaatcagat ggtgtagaag tttctaccgg tgcattctca agaaccgtca tagatcctgt
     1561 tacaggtcag aaagtaacag aaacatttga caatggtaac tggatgtttg gtgtaaacta
     1621 caagacagat ccatcagaaa cacgtctaga tgacgcaggt caaaacaagg gtaaagacac
     1681 tcgttgggat cttggtggtc tggagatgtc tatggcaaat ggtaatgcta agaacgaaca
     1741 acatgcccgt tactggtata ctgaaaaagg tggcgttgat gtttactaca tcacttcgga
     1801 cggtaaggtt cttactgact tcactacttc tgatggaaaa actgtagcag acaaggtaac
     1861 gatagtgggt cacggaaaca ctgattcagc ttacgataca acttcagtac gttatccaac
     1921 tattacggct gcagacggta cagtttacta ctacaaagaa attgatactg cagcaaaaaa
     1981 tctccaccct gttgtagatg acaagacgga tacagattac cgatcaaatg aaaaaattga
     2041 tgcagaaaaa ggaacaatta agactgatac agtcaaacaa ttgacttatg tttacgaaaa
     2101 agctggtaat gtcaatgtac actatgtaga tacagacggt aatgaattgc aatcgtcagt
     2161 tgaagatgtg acaaatggta aaccaggtac tggctatgac accgttttag ataacaagca
     2221 aaaagaaatc acagtaaatg gtacagttta ttatcttgtc ccagctggag agtacaaagt
     2281 tggtacagtt ggtgatgaaa ataacctgac tgtagttggc aacggaaaag caactggtat
     2341 tgatgctact acgggtactg tagatgctgg cgtgactaaa aatgtaactt acgtttacca
     2401 aaaaggtggc gatgttattg tcaattatgt tgatcaagat ggaaatccaa tttctggtac
     2461 aacagataaa ggtacaacaa ctgaaagtac cgtaacagat acaagctata ctaaagctgg
     2521 tactgcctac aatacaactg atttgaaacc aacaactatc acaaccacag atggaaaagt
     2581 gtaccaactt gttccagaag caactaaagg taatgaagat ggtacagttg agtcaggtgt
     2641 aacaaaagaa gttacttacg tctacaaaga agtcaaaggt tcagttgttg taaattacat
     2701 ctcaacaaca ggtgaagaaa tacaagcaca agtagtagat acaccagaat cttcaacagg
     2761 tacagagtac gatacaacag atgtgaagcc agaaacaatc acaactaaag atggtcgcac
     2821 attcaagttg gttcctaaat taactcaggg atctgaaaaa ggtgaagttg ttccaggcgt
     2881 aacaaaagtc acttatgtct acgaagaagt caaaggtgat gttgttgtta actacgtaaa
     2941 tactgatggt aaagtaattg ctacccaagt agtagataca aagacaactt caacaggtac
     3001 agcttatgat acaactgaca acaaaccaga gaaaatcgtt gaagatgcga ctggtgatgt
     3061 atactactac aaagaagttc aggctggctc aaacgaaaca ggtaaggttg tagaaggtac
     3121 aacagaagtt acttatgt
//
LOCUS       DATUCG010000189         3132 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.192, whole genome shotgun sequence.
ACCESSION   DATUCG010000189 DATUCG010000000
VERSION     DATUCG010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.192"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            37..588
                     /locus_tag="VCA20_002313"
     CDS             37..588
                     /locus_tag="VCA20_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEP1836037.1"
                     /translation="MTAILQEEIATSNIFIDKASGKDFDRRNYKRLLRKLRTGDELDI
                     KSIDRLGRNYDEIIEQWRLLVKKKQVDISVLDFPLLNTRNGIENVTGKLISDLVLQIL
                     SYVSQIEQEQIQQRQKEGIAEAMKEGVRFGRPKLEKPFLFDDIAQAYQNGEISVQEGA
                     KVLNVPKSTFHNWLKDENYCPKE"
     gene            773..1537
                     /locus_tag="VCA20_002314"
     CDS             773..1537
                     /locus_tag="VCA20_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEP1836038.1"
                     /translation="MTKNFTTKAAKVLTIATLSAGTLIGAIALDTTTVSASSVSQISG
                     DELKGKIQAVTNSFPTFESAQAFVTGQMSQMLFGESYRAVVIPENSLYIVEVTIDYDA
                     LTSKDQAIALANQVVAAADSAIGGAAPATPAEPEMPVTSEEPTTPVEPETPVTPEEPT
                     TPTEPVVPEEPTTPIEPETPVTSVEPTTPIEVSKNQKSSTTKEEIKKVIPDNSTEAIK
                     KDVKTLPNTGDAGSVLSIIGSGLLSLGGYVALKRKN"
     gene            1785..2513
                     /locus_tag="VCA20_002315"
                     /pseudo
     CDS             1785..2513
                     /locus_tag="VCA20_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406008.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
BASE COUNT         1112 a          605 c          536 g          879 t
ORIGIN      
        1 agagtctcca caaaggagca acacattgac agacaaatga cagcaatttt gcaagaagag
       61 attgccacaa gcaatatttt tattgataaa gcttctggca aagatttcga tagaaggaat
      121 tataaacgat tgttaagaaa acttcgaact ggtgatgaac ttgacataaa atccattgac
      181 agactaggaa gaaattacga tgaaatcata gaacaatgga gattattggt caaaaagaaa
      241 caagtcgata tctctgtgct agattttcca ctcctaaata ctcgaaacgg aatagaaaac
      301 gtcacaggaa aactaatctc agaccttgtt ctccaaattc tttcctatgt gtcacagatt
      361 gaacaggagc aaattcaaca aagacaaaaa gaaggaatcg cggaggctat gaaagaaggg
      421 gtgaggtttg gaagaccgaa attagagaaa ccttttctgt ttgacgacat cgctcaagcc
      481 tatcaaaatg gagaaataag tgttcaagag ggagcaaaag tattaaatgt tccgaagagc
      541 acctttcata attggctaaa agatgaaaat tattgtccaa aagagtagga gtttttagac
      601 atcagctgta tctgttattt ctaactcaga tgacttcaag aaaaatctaa aaaacattat
      661 aagagtaagc ttttctgtca acaagtatat tttaacaaaa ccgtcccaaa tagtctgctt
      721 tttgggacaa gttaagtcta tttctgttta aattatctag gagggtcatg atatgactaa
      781 gaatttcaca accaaggccg caaaggtctt aacaattgcc actctttctg cgggaacact
      841 cattggagca atcgcattag atactactac cgtttcagct tcatctgtct cacaaatctc
      901 tggagatgaa ttgaaaggta aaattcaagc tgtcactaac tcatttccga cattcgagtc
      961 tgctcaagca ttcgtaaccg gtcaaatgtc tcagatgtta tttggggagt catatcgtgc
     1021 agtcgtgatt ccagaaaatt ctctctatat cgtagaagta acgattgatt acgatgcttt
     1081 gacttctaaa gaccaagcaa tcgcattggc taatcaagtt gttgcagctg cagattctgc
     1141 aatcggcgga gctgcacctg cgactcctgc tgagccggaa atgcctgtga cttcagaaga
     1201 gccgactact ccagttgagc cagaaacacc tgtgactcca gaagagccaa ctacaccgac
     1261 tgaaccagta gttccagaag aaccaactac tccaattgag ccagaaacac ctgtaacttc
     1321 ggttgaacct actaccccta ttgaagtttc gaaaaaccaa aaatcttcta caacaaaaga
     1381 agaaatcaaa aaagttatcc ctgataattc aacagaagca atcaagaaag atgttaaaac
     1441 attgccaaat acaggcgatg caggtagcgt actgagtatt attggtagcg gtttgctttc
     1501 actgggtgga tatgtagccc taaaacgaaa aaattaacga ctttgccttc tctgccttag
     1561 atagagaggg cttttactaa attagaagga aaataaatga gcatacgacg ctttttatac
     1621 gaaaacatta gcagacggct aaagcatcaa atcaatagta agcataatgg gaagaacaag
     1681 gaactaattg cctatttaga aaaatgctac ctcgtctaga tgaagaagat gataaggtat
     1741 ggaccgaatg tactctatct aaaatattaa acggaaaatc ttcgcttagt cttactgatt
     1801 tagatatatt agcatcgaca agtatgcaga aatccattca aagaaaacaa ctcacgaaat
     1861 aaaaacttca ttgcgtgatg cttatgctcg tggctatatc tcaacagatt ttctttactt
     1921 agttaaggca aggggcatat tcccataaaa cgaaataagc ctttatctgt caccaatttt
     1981 caaaaactac gtacttatct tttgaacaat caaaaagatg agttccatat tctcgtactt
     2041 ttagcacttg aaataggtat gcgttgagga gaattattgg cattcgacct gaatatctgt
     2101 ttgattacgg aatcaaagtc agagaatcta ttagtccaac ctctagtgat acttcgctaa
     2161 aaactgaaaa ttcaagacga gaggtcacaa tcaataaaga agtctatgac ttactcctat
     2221 cagtttcagt aaaagaaaat ggatatttct tcgatcctga tggatttcat caatctcgaa
     2281 agttagccaa gttactggaa gaactaggaa tctcaaaaat cacatttcac ggtcttcgag
     2341 atactcatgc ttcatcttta ttctcaaagg atgttgacat catctatgtt tcaaaaagac
     2401 ttggtcatgt caatgttcaa actacacaga actattatct ggagttaatg cctgaaaaaa
     2461 gacaccagca agatgccgat gccttaaatt tattgaattc attaagcgag taaacatttg
     2521 aaccaatttg aaccaaaaag tcggcacaaa tgttgtcccc ataacgtttg gattaacgtt
     2581 tagagaattt cgctattgaa aacgaagact tctcacccct tacaaatccc gttctatcaa
     2641 catttacgaa aatgaaactt ccatgaaaat ggactaaaat cctctgattt ggataaaatt
     2701 gacaccaatt aacaccataa aatagactag tcatcagtag gtcaaactaa tttgacctac
     2761 tttatcttat agatggataa tttattaatc aaattcaaaa tgataggtca agtagccttt
     2821 ttgtaaacca ctttagtcgc atttttccaa agatacctct aaaataattt ccaagcttct
     2881 ctatcaataa attttcaatt aatgaaataa tcagcatatg taatgtcctc tctcactaag
     2941 tatatcatac ccccatagga atacatctac tacgattttt gtatcgcttt atcatctcgt
     3001 aattaattca ttgataaagt tttctcaaaa tacagctgaa accccttcaa cacttaatgt
     3061 aaccgcccat aacaaggtat ctatccaatc accccttcct ccagtatact gttataaaaa
     3121 acaaactgga gg
//
LOCUS       DATUCG010000190         3110 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.193, whole genome shotgun sequence.
ACCESSION   DATUCG010000190 DATUCG010000000
VERSION     DATUCG010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.193"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            43..741
                     /locus_tag="VCA20_002316"
                     /pseudo
     CDS             43..741
                     /locus_tag="VCA20_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522433.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
     gene            complement(805..2823)
                     /gene="recG"
                     /locus_tag="VCA20_002317"
     CDS             complement(805..2823)
                     /gene="recG"
                     /locus_tag="VCA20_002317"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEP1836039.1"
                     /translation="MKRLCDELSVLPGIGPKSAEKFLKINIQNINDLLTYYPFRYEDF
                     ESKSIYDLQDGEKAVVVGEVVSPANVQYYGYKRNRLRFSMKQGEVVLAVSFFNQPYLA
                     DKIVLGQDIAVWGKWDKAKASLTGMKILAQVSDELQPVYHVAQGISQVNLVKAIKTAI
                     DQGYLHLLEENLPSVLRERYRLMNRREAVFAMHFPTNLEEYRQALRRMKFEELFYFQL
                     QLQMLKANNRDISNGLKIAYDADRLAMQIRQLPFVLTDAQSGALAEILSDMKSYGHMN
                     RLLQGDVGSGKTVVAGLAMFAAVTAGMQAAIMVPTEILAEQHFESLRQLFPELSIALL
                     TGGMKAAERRTALEAISSGQVDIIVGTHALIQESVTYHKLGLVVTDEQHRFGVKQRRL
                     FREKGDNPDVLMMTATPIPRTLAITAFGDMDVSIINQLPAGRKPIITRWVKHQQLPTV
                     LDWLEQELEVGAQVYFISPLIEESEALDLKNAVDLQSDLQAHFGEQVTVDLLHGKMKN
                     DEKDAIMQAFKERKTNILVSTTVIEVGVNVPNATVMVIMDADRFGLSQLHQLRGRVGR
                     GHKQSYAVLVANPKTESGNERMKIMTETTDGFILAEADLKMRGSGEIFGTRQSGLPEF
                     QVANIIEDYPILEEARRVASQIVSVENWQEDPNWSILLANLKDREELD"
     gene            complement(2942..3013)
                     /locus_tag="VCA20_002318"
     tRNA            complement(2942..3013)
                     /locus_tag="VCA20_002318"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2978..2980),aa:Glu,seq:ttc)
     gene            complement(3036..3108)
                     /locus_tag="VCA20_002319"
     tRNA            complement(3036..3108)
                     /locus_tag="VCA20_002319"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3073..3075),aa:Asn,seq:gtt)
BASE COUNT          971 a          784 c          509 g          846 t
ORIGIN      
        1 atctcatttt tgggaaattt agggagtttt tagaggtgcc cagaagaaac tgcctatttc
       61 cttgatacaa tggctattcc agaaaaacca attgtattga ccggagccat gcgttcctca
      121 aatgaactag gtagcgacgg gatttataac taccgaacag ccctacgtgt ggctgctgat
      181 gataaatccg cagataaagg tgttctagtg gtcatgaacg atgaaattca tgctgctaaa
      241 tatgttacaa aaacgcatac caccaatgtc tcaacctttc aaacaccaac ccatggacca
      301 ctggggttag tcaccaaacg agagattctc tattttaaaa ctgctgaacc acgtgttcga
      361 tttgacttat caacagtcac aggaacggtc ccaatcataa aagcttatgc agatatggac
      421 tccattctcc ttgattctct cactgcaagc tcaatttccg gtttagtcat tgaagcactc
      481 ggcgccggaa atcttccgcc taccatcctt ccagccatta agaaactcct tgttcaaaag
      541 atacccatta ttctggtatc tcgctgtttt aacggaattg ctgagcctgt ctatgcctat
      601 caaggaggtg gcatccaact ggagcaagac ggcattctat ttgttaaaga attgaatgct
      661 caaaaagccc gactcaaact ccttatcgct ctcaatgcag gcttggaaga gcaaagcttg
      721 gcagattaca tccaaggtta atcgctgacg aactatacaa tatgccaaaa gaccaacaca
      781 ggtgtgttgg ccttttttat atgactagtc caattcctct cgatctttca aattagcaag
      841 caaaatcgac caattagggt cctcctgcca attttccacg ctaacaatct gactagcaac
      901 tcgtcttgct tcctctaaaa ttggatagtc ctcgataata tttgcaacct gaaattctgg
      961 caaacctgat tgacgagtcc caaatatttc tccagaccca cgcattttta agtcagcttc
     1021 agccaaaata aaaccatctg tcgtctctgt cataattttc atgcgttcat taccagactc
     1081 tgttttagga ttagcaacta acactgcata agactgctta tgaccacgtc ctacacgccc
     1141 tctcaactga tgtaattggc ttaaaccaaa acgatctgca tccataataa ccatcacagt
     1201 tgcattgggc acattgactc cgacttcaat aacagtggta gaaaccaaaa tattcgtttt
     1261 tcgctcctta aaagcctgca tgatggcatc tttctcgtca ttcttcatct taccgtgtaa
     1321 caaatccact gtaacttgct ctccaaaatg cgcctgtaaa tcagattgca aatctacagc
     1381 attctttaaa tctagagcct cagattcttc aattagcgga gagataaaat aaacctgagc
     1441 acccacttct aactcctgct ccaaccaatc taagaccgtg ggcagctgtt gatgtttgac
     1501 ccagcgagtg ataattggct tccgaccagc aggtaactga ttaataatcg atacatccat
     1561 atctccaaaa gctgtaatgg ctaacgtccg aggaataggt gtagcagtca tcataagcac
     1621 atccggatta tcacccttct cacgaaacag acggcgctgt ttaacaccaa aacgatgttg
     1681 ctcatccgtc acaaccaaac caagcttatg atacgtcaca ctttcctgta taagagcatg
     1741 agttcctaca atgatgtcta cctgaccact agaaatcgcc tctaaagcag tacgacgctc
     1801 cgcagccttc attccacctg tcaaaagagc aatagaaagt tctggaaaaa gctggcgcaa
     1861 actctcaaag tgttgctcag ccaaaatttc tgtcggcacc ataattgcag cctgcatccc
     1921 cgcagtcaca gcagcaaaca tagccaatcc agcaactact gtcttccctg aaccaacatc
     1981 cccttgtaac aaacgattca tgtgcccata ggacttcata tctgatagta tttcagccaa
     2041 agccccagac tgagcatctg ttaagacaaa cggcaattgt cttatttgca tagccaatct
     2101 atcagcatca taggcaatct tcaaaccatt cgaaatatca cgattgttgg ctttcagcat
     2161 ttgcaactgt aattgaaaat aaaataattc ctcaaacttc atacgtcgca aagcttgtct
     2221 atactcttct aaatttgtag gaaaatgcat cgcaaaaacc gcctctcggc gattcattaa
     2281 ccgatagcgc tctcgtaaaa ctgatggcaa attctcctct aataaatgca aatatccctg
     2341 atcaatagct gttttaatag ccttaaccaa atttacttgc gaaatccctt gcgccacatg
     2401 atagacaggt tggagctcat ctgatacttg agccaaaatt ttcattcctg tcaaacttgc
     2461 cttagcctta tcccactttc cccaaactgc aatatcctgc cccaaaacaa tcttatctgc
     2521 taaataaggc tgattaaaaa acgaaactgc taagacaacc tcaccctgtt tcattgagaa
     2581 acggagtcta tttcttttgt aaccataata ctgtacatta gctggagata ccacctcccc
     2641 aactaccact gccttctccc catcctgcaa atcataaata gacttactct caaaatcttc
     2701 atatctaaat ggatagtaag tcagcaaatc attaatattc tgaatgttaa tttttaagaa
     2761 tttttcagcc gattttggac caatccctgg caaaaccgat aattcatcac ataatctttt
     2821 catgtctcta ttatatcaac aaacaattaa tagacaaagt aacaagaaaa gaaaaaggag
     2881 ccaaattaaa gcacattcac ctcaaaaaac aaaaaaaaga gacctaagca gcctccaaat
     2941 atagtccgta cgggattcga acccgtgtta ccgccgtgaa aaggcggtgt cttaacccct
     3001 tgaccaacgg accatatact aaagattttt ctttactccg ccaacagggc tcgaacctgt
     3061 gacatcatga ttaacagtca tgcgctctac caactgagct atggcggaat
//
LOCUS       DATUCG010000191         3089 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.194, whole genome shotgun sequence.
ACCESSION   DATUCG010000191 DATUCG010000000
VERSION     DATUCG010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3089
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.194"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            168..2432
                     /locus_tag="VCA20_002320"
     CDS             168..2432
                     /locus_tag="VCA20_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GBS Bsp-like repeat-containing protein"
                     /protein_id="HEP1836040.1"
                     /translation="MSSLSNSNIAVSQRTSLTVANEGAKQSKTLVAENTTVSSLQTPS
                     SEIVGSANSMSELTNQGESISDSTTISNEVKPDQETTSSNAISVTSGVSLTKENASVA
                     SSTSSTSAGTGNTVTTPSTNVAARSTVSSSSQSTTSTQASNVVAIENVNAVNGSFDVR
                     VSKVSSPKEIKEVYIPTWSEVNGQDDIIWYAAKRQLDGSYLINVDNAQHKKSVGKYHS
                     HVYYLATDGSLSAIGATSAVLPELKASGKVSAVNVNQGAGSFEIKISDIVAPKGIAKF
                     LVPTWTESGGQDDIVWHEASRQSDGSYLARINKNQHKNELGKYISHVYLLEQDGSLSG
                     IGATSAVLPELKASGKVSAVNVNQGAGSFEVKISDIVAPKGIAKFLVPTWTESGGQDD
                     IVWHEASRQSDGSYLARINKTQHKYESGKYISHVYYRGHDGSMTAIGGASVELPELKL
                     SGTITVSDVNVQAGSFNVRITNISSPRGLDKVYVPTWTESGGQDDIVWHEASRQSDGS
                     YLARINKNQHKYSSGKYISHVYYRGTDGSMTAVGGTSVELPSTRSYTVYIDPGHGGRD
                     SGASYGGVHEKNLALSVSNKLRENLLKYGINVLMTRTGDYDVDFKTERSRMTNASNAD
                     LFISIHFNATGAGVSNSSGIETYWYQYDPEYQPKINKEMHNNPTRLAESEILANKVQE
                     SLIKETGAVNRGVRRETFAVLRETAIPAILVELGFMDNPSELQVIKQDSYHTRLAKAL
                     AQGVMNWYGAVEGK"
     gene            2552..2938
                     /locus_tag="VCA20_002321"
     CDS             2552..2938
                     /locus_tag="VCA20_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEP1836041.1"
                     /translation="MLSQAILESAWGTSYLATHGNNLFGIKADAAWTGDTIEIITNEY
                     RDGEKKQEKHLFRKYNSWNESVADYAKFFTSTPWRIKNYQSFREATDYQQAILALRQS
                     GYATDPKYGEKLRSIIENYKLYYLDS"
BASE COUNT         1035 a          534 c          657 g          863 t
ORIGIN      
        1 attaaaaata gttataatca caatttttaa tattgattga taaggagtat tttgtgaaaa
       61 agaaatcatt attatttctt tccttgtgtg cttggacact tcaaagtcat ctcgttcaag
      121 cagataccat agtggatact gcaagccaag catcgcaaga aacagaaatg tctagtttgt
      181 cgaactcgaa tattgcagta agtcaacgaa catccctaac agttgcaaat gagggggcga
      241 aacaatcgaa aacgttagta gctgaaaata cgacagtttc aagtcttcaa acgccttcat
      301 ctgagattgt tggctctgct aacagcatga gtgaattaac aaaccaaggc gaatctatat
      361 cagatagtac aactatttca aatgaagtaa aacctgatca ggaaacaact tcttcaaatg
      421 ctatttctgt aacatcaggc gtttctttga caaaagaaaa tgcttccgta gcaagctcga
      481 caagttcaac ttctgcaggc acaggcaata cagttacaac gcctagtaca aatgttgctg
      541 ctagatctac ggtttctagt tcaagccaat caacgacaag tacgcaggca tctaatgtag
      601 ttgcaatcga aaacgttaat gcagtaaatg ggagttttga tgtacgtgta tcaaaagttt
      661 cttctcccaa agaaattaaa gaagtatata ttccaacttg gtcggaagtg aatggtcaag
      721 atgatattat ttggtatgca gccaaacgtc aattagatgg cagttaccta attaatgtag
      781 ataatgccca acataagaaa tctgttggaa aatatcattc gcatgtgtat tatctagcta
      841 cagatggtag tttatctgca atcggtgcaa cctcagcggt attgccagaa cttaaagcca
      901 gtgggaaagt ttcagcagtg aatgtcaatc aaggtgcagg tagctttgaa ataaaaatta
      961 gcgatatagt cgcgcctaaa gggatagcta agtttcttgt tccgacctgg acagaatcag
     1021 gaggtcagga tgatattgtt tggcatgaag catcacgcca gtcagacggt agctaccttg
     1081 caagaattaa caaaaaccaa cataagaatg aattaggcaa atatatttcc catgtatatt
     1141 tgctagagca agatggtagc ttatcaggta tcggtgcaac ctcagcggta ttgccagaac
     1201 ttaaagccag tgggaaagtt tcagcagtga atgtcaatca aggtgcaggt agctttgaag
     1261 taaaaattag cgatatagtc gcgcctaaag ggatagctaa gtttcttgtt ccgacctgga
     1321 cagaatcagg aggtcaggat gatattgttt ggcatgaagc atcacgccag tcagatggta
     1381 gctaccttgc aagaatcaat aaaacccaac ataaatatga atccggtaaa tatatttctc
     1441 atgtttatta ccgaggtcac gatggaagta tgaccgctat tggcggagct agtgttgaat
     1501 tgccagaatt aaaactttct ggcaccatta cagtttcaga tgtaaatgtt caagcaggta
     1561 gtttcaatgt tcgcatcact aatatttcat caccgcgtgg tttagacaaa gtctatgttc
     1621 cgacctggac agaatcagga ggtcaggatg atattgtttg gcatgaagca tcgcgccagt
     1681 cagatggtag ctaccttgca agaattaaca aaaaccaaca taaatatagt tcagggaagt
     1741 acatttcgca cgtttactac cgcggaacag atggaagtat gacagcagtt ggcggtacta
     1801 gtgttgaatt accatcaaca cgctcctaca cagtctatat tgacccagga catggagggc
     1861 gagactcagg agcttcttat ggaggtgtac atgaaaaaaa tcttgcattg tctgtttcaa
     1921 ataagttgcg tgaaaatctc ttgaaatatg gaatcaatgt tttgatgacc cgtacaggcg
     1981 attatgatgt agacttcaaa acagagaggt cacgaatgac gaatgcaagc aacgcagact
     2041 tatttattag tatccatttt aatgcaacgg gtgcaggagt ttcgaattcg agtggtatcg
     2101 aaacatattg gtatcaatac gatccggaat accaacctaa aattaataaa gaaatgcata
     2161 ataaccctac tcgtttagct gaaagtgaga ttttagctaa caaggttcag gagagtttaa
     2221 ttaaagaaac aggtgcagtt aaccgtggcg taagaagaga aacttttgca gtccttcgcg
     2281 agacagcaat tcctgctatt ctagtggagc taggatttat ggataatcct tcggagttac
     2341 aagttatcaa acaagattct taccatactc gccttgcgaa agcattggca caaggtgtga
     2401 tgaactggta tggtgctgta gagggaaagt agggactgca ggtacaggta ttgaaacagg
     2461 aaaactttct aatgatcaaa aaagtttcat agctaatcta gtgcctgcaa ttgagcaaaa
     2521 ttcaaaagtg aattctgtcc tttcgagtgt catgttatct caagccatct tagaaagtgc
     2581 atggggaacg tcatacttgg cgactcatgg gaataatctt tttggcatca aagcggatgc
     2641 tgcatggacg ggtgatacaa ttgaaatcat taccaatgaa taccgtgatg gtgagaagaa
     2701 gcaagaaaag cacttgttta gaaaatataa ttcatggaat gaaagtgtag cagactatgc
     2761 taagttcttt acctctactc cgtggagaat aaaaaattac cagagtttca gagaggcaac
     2821 tgattatcaa caagcgattc ttgcgctaag acaatcagga tatgctactg atccgaaata
     2881 tggtgaaaaa ttacgatcaa ttattgaaaa ttataaatta tactatcttg atagctaact
     2941 caacaaattg tcttgactat tcgcctatcg ggataaaatt atgataaatt tttctattta
     3001 aaataaaatt tgaaaagact acttatggta tgcagttaaa ttgtagtgga ctgtcatatt
     3061 aagtggtctt tttattgcta aaataagtt
//
LOCUS       DATUCG010000192         3041 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.195, whole genome shotgun sequence.
ACCESSION   DATUCG010000192 DATUCG010000000
VERSION     DATUCG010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.195"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            421..1473
                     /locus_tag="VCA20_002322"
     CDS             421..1473
                     /locus_tag="VCA20_002322"
                     /inference="COORDINATES: protein motif:HMM:NF012606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol-specific phospholipase C"
                     /protein_id="HEP1836042.1"
                     /translation="MKKKITALLLGLMMVFGVSTNVYAHYDRAYWHEEKETTPYIEQY
                     VDWMITIPDDRRLSDLSLPGTHDTMAYNGNLAFGNIARTQTMNLEQQLNSGIRYLDIR
                     LAHRGSYFQLHHGAVDLGYNFDDVLQTVISFLESHPTETVLMRIKQEHTSENDRKMLE
                     LFTKYYEKYKESFWDREKSETPYNPKLSEFRGKILILSDVYSIYQGINYREVIKQDEY
                     HLGSNWDLYRKWESVKSQIEKSDKEYPDKIMMNYLSGSGGAYPYFVASGHVNPDTYAS
                     RLSTGLTEPGFKNYYPDFPRTGRFGVFATISFEGTNTLTADYLRSQGIKRAGIVAADF
                     PGERLIDEIIQCNYRK"
     gene            1495..>3041
                     /locus_tag="VCA20_002323"
     CDS             1495..>3041
                     /locus_tag="VCA20_002323"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019941.1,HMM:NF037291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HEP1836043.1"
                     /translation="MRKEKKWLHRISSMILSFLMVLQVFSGSFGNMQVHAQNDADIWQ
                     GIGENIGMPWNAIYITHPKYTEFKLQMFCIEAKKEFPKENAPIEYELIKPAKDDLIKK
                     SFNQPNTNADVALEGVKRVLWNGHPWNASGLKEKYDVTEEEFQLVTTMAMQYWTDSYW
                     GNKGSNYIHDNHEAFKIDSQRKREKKLLLVDMFKELVGLGEYVLSDPPSDAEFLLGIP
                     HGVEYARSYQKILSMDLKKYPIHFIKVNENGEKISGARLKVTKLDPPFTSIAVGDDYN
                     LEWTSDEKKEGELGYFAFGSYKLEEIKTPDGGYVPFDKFNFNIVPGGKIEFPGGKPSY
                     VTGVETRIEELNGYEQTVSYFTIKNEKTEAPKPQYKGGKLKTTVKVDNKSESEQEVQA
                     GKNLTVVIGEKAAKKDVVDTVAYSDLVGGAEYELTGKLMKIVDGKEPEQVGEAVKKTF
                     KADVSGSGTEEMTFKDVNIEVGVKYVVFEEAVSKEKIEFKEETKKHIVTHHDKDDKSQ
                     TFNVEKE"
BASE COUNT         1153 a          407 c          683 g          798 t
ORIGIN      
        1 attgtatttt gaaatgaact agcaccacga gtaaagatat aagttaaaat tacatggaaa
       61 taggatttat tttaaagaaa tttgtatgaa caattgggga acaattgtaa ctcaacaaaa
      121 ttcgtaaaca aagtttacat aagtcttcat ctttttataa aattatggta taattaagaa
      181 aaatttgggt atagaaaatg aataaaagcg aagaaaagag ttgacaaggg aaatgttaaa
      241 agcgataatc gttagacaga cagacagaca aggtagcatc tgtctttttg gcatagaaaa
      301 atttgaatat acatagctaa aaagtagagt aactctatat aaatagggat attctacttt
      361 tttgcgtttt taagcaggta tattcaaatg aaaaaatgaa tttaagagga ggtgaaagaa
      421 atgaaaaaga agattacagc acttttgctt ggtctaatga tggtctttgg agtttctaca
      481 aatgtttatg cccattatga cagggcatac tggcatgagg agaaagaaac cactccgtat
      541 atagaacaat atgtagactg gatgattact atacctgatg atcgaagatt aagcgacctg
      601 tctcttccag gtacacatga tacgatggca tataatggca atctcgcctt tggtaatatt
      661 gcaagaaccc agactatgaa cttagaacag cagctaaatt caggaattcg ctatctggat
      721 atcagacttg ctcatagagg aagttatttt caactgcatc atggagctgt tgacttagga
      781 tataactttg atgatgtatt acagacagtc atatcttttt tagaaagtca tccgacagag
      841 acagttctta tgagaattaa gcaggaacat acaagtgaga atgaccgtaa gatgcttgaa
      901 cttttcacaa agtattacga aaagtataag gagtctttct gggatagaga aaagagcgag
      961 accccataca atccaaaact atctgagttt agaggaaaga tattgattct ttcggatgtt
     1021 tattctattt atcaaggaat caattacaga gaagtcatca aacaagatga atatcatctt
     1081 ggaagtaatt gggatttata taggaaatgg gagtcggtta aatcgcagat agaaaaaagt
     1141 gataaggagt atcctgacaa gattatgatg aattatctaa gcggatcggg aggagcttat
     1201 ccctactttg tggcaagcgg acatgttaat ccggacacat atgcaagtag actgtcaacg
     1261 ggactgacag agccgggatt taagaattac tatcctgact ttccaagaac aggacgattt
     1321 ggagtatttg cgacaatatc ctttgaagga acgaatactt tgacggctga ctacttacgc
     1381 tcacaaggta tcaaaagagc agggattgta gcagcggact ttccggggga aagactgata
     1441 gatgagatca tacaatgtaa ttatagaaaa taggaaaaga aaaggagaga aggtatgaga
     1501 aaagagaaga aatggctgca tcggataagc tcgatgattt tgtcattctt gatggtactg
     1561 caagtattca gcggctcttt tggcaatatg caggttcatg cacagaatga tgctgatatc
     1621 tggcagggta taggtgagaa tattggaatg ccatggaatg caatatacat tacacatcca
     1681 aaatatacag agtttaaact tcagatgttt tgtattgaag caaagaaaga gtttccaaaa
     1741 gaaaatgccc caatagaata tgaattaata aaacctgcca aagatgattt gattaagaaa
     1801 tcttttaatc aaccgaatac aaatgcagat gttgcattag agggagttaa aagggttctg
     1861 tggaatggtc atccatggaa tgcaagtgga ttaaaagaaa agtatgatgt cactgaagag
     1921 gaatttcaat tagtgacaac aatggctatg caatactgga cggatagcta ttgggggaac
     1981 aaaggatcaa attatattca tgataatcat gaagcattca aaattgattc tcaacgtaaa
     2041 agagagaaaa aactattatt agttgatatg tttaaagagt tggtagggct tggagaatat
     2101 gtattatctg atccaccatc agatgctgaa tttttattgg gaataccaca tggtgttgag
     2161 tacgccagat catatcagaa aatattgagt atggatttaa aaaagtatcc gatacatttt
     2221 attaaagtta atgaaaatgg cgaaaaaata agtggtgcga gactgaaagt tactaagtta
     2281 gatccgccat tcacatcgat agctgttgga gatgattaca atttagaatg gacatctgat
     2341 gagaaaaaag aaggtgagtt aggctatttt gcgtttggta gctataaact tgaagaaatt
     2401 aaaactcctg atggaggata tgttcctttt gataaattta actttaatat agtgccgggt
     2461 ggaaaaatag aattcccagg cggtaagcca agttatgtta caggagtgga aacacgcata
     2521 gaggaactga acggttatga gcagacagtt tcttatttca ctatcaaaaa tgaaaaaact
     2581 gaagcgccaa aaccacagta caagggtgga aaactaaaga ctacagtaaa ggtagataac
     2641 aagtcagaaa gtgaacaaga agtacaagca ggaaagaacc taacggtagt gataggagaa
     2701 aaggctgcta agaaagatgt agtggataca gtagcctaca gtgatctggt aggaggagca
     2761 gaatacgaac taacaggaaa actgatgaag atcgtagatg gtaaagaacc ggaacaagta
     2821 ggagaagcag tcaagaagac attcaaagca gatgtaagtg gaagtggcac agaagagatg
     2881 acatttaagg atgtcaacat cgaagtggga gtgaagtatg tagtctttga agaagcagta
     2941 tcgaaagaaa agatagagtt taaagaagag acgaaaaaac atatagtgac acaccatgat
     3001 aaagatgata agtcacaaac ctttaacgta gagaaagaga a
//
LOCUS       DATUCG010000193         2748 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.196, whole genome shotgun sequence.
ACCESSION   DATUCG010000193 DATUCG010000000
VERSION     DATUCG010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.196"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            686..>2748
                     /locus_tag="VCA20_002324"
     CDS             686..>2748
                     /locus_tag="VCA20_002324"
                     /inference="COORDINATES: protein motif:HMM:NF019202.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HEP1836044.1"
                     /translation="MNRSKKKSFDWYGMRQRFSIRKYHIGAASVLLGMTLVLSAGTIV
                     SAETGVNGDGASNEQTLPVNDAGTSDGTNSTTQQQSDNQGNNSSGVQNDNTTNSTANV
                     QPAGDTGSASGLKTKNPRTMVLKPEGDGSTNPTVDEPIIKFPTQDNQTQPAGYQRVVF
                     YSSFATLVFEDKPGEIVITRNGKPVKVVTFWVKTGTTWEKLKKDPQFRIPTYQLQDGD
                     FILGYDRSVAQKTQHIDFDSLNDTPVEETWLSPTVDYIVGDVDDGKTFEEYMKSGYAP
                     FHIPQFHGITLDANGGTVGGKAKKAFYVAYEHVKWGDELVTKKIVDPSRDGYTFVRWQ
                     TATGGGIPTSGQIETIEFTAVWLKHPTEAVKVFNPEQLSETDKTKVADLIRSVNPDLQ
                     NSIQEITVANNGDATVRFTTGDERTISGASLVSDNSAEQRAKAEESISDAVLKKQDDI
                     AKSQLTAKEQQDKLAELTKLQESIEEQIEGATSEDALDKIVKDGKNSIENFDLTSATK
                     KVAAKEALEEAYNAKKDEIDNSSLTAEEKAAKQAELDKAKEDADKAIDTATDDAGVAQ
                     ALTDGKTAIAGIDISTSAKKAQAKQDLETAYNAKKDEIDNSSLTAEEKVAKQAELDKA
                     KADADKAIDAATDDAGVAKALEAGKATIDAINTTTSAEKAAAKEALEEAYNAKKAEIE
                     NSALTA"
BASE COUNT          937 a          462 c          613 g          736 t
ORIGIN      
        1 tctccttatt actttttatt ctattatctc atttttggga aatttaggga gtttttagag
       61 gtgccctatt acaatttgat tagtagatag ctaattttga ttttaaatga gtattaaaat
      121 tactaatctc taaagtgagt tgcaattaat ttgtgaatga taatcgggtc aattccttga
      181 gcttttggta tttttggtag gtatcatcag tatagtgtct gtttacatag cgctgtttaa
      241 aaagcagata aatgagttca tgtaattttt cttttatcgt gatgtcgaaa tagctacctt
      301 cttcttggat tctggaactc tagtcatcaa tgaacttgtg gcacttgact tgactaaata
      361 atgtgcagaa attccttgaa aaaatggggg gggtatgtta tactggaatg taaagtatac
      421 tttttgtaaa gtaactccgt taagatgtcg ttggtttgat ggggatgcgc gggagttggt
      481 caaactgtcc tgtaaagtta gattttttgt ctcacttttt ttaaggtcag tgcaaagatc
      541 agagtcgctt tcaataaatc gtgtcagcag attcgcactg agaggttgct tatcaatgag
      601 tgttactcaa gagaagatga tgtgttaatg aactaattgg tcataacgtg tattctctgg
      661 aaaataatac aaaaggagtg acgatatgaa tcgttcgaaa aagaaatcat ttgattggta
      721 tggaatgcgt cagcgtttct ctatccgtaa atatcatatt ggtgctgcaa gtgttctgtt
      781 aggtatgacc ttggttctta gtgcaggaac gattgtaagc gctgaaacag gagtcaatgg
      841 ggatggagct tcaaacgagc aaactttacc tgtaaatgat gctggaacat ccgatggtac
      901 aaattcgaca acgcagcaac aatcggataa tcaagggaac aatagctcag gagttcagaa
      961 tgataataca acaaactcaa ctgcgaatgt ccagcctgca ggtgatacag gttcagcaag
     1021 tggcctaaaa acaaaaaacc cacgtacaat ggttctgaaa ccagaaggag atggttcgac
     1081 taacccaact gtagatgaac cgattatcaa atttccgact caagataacc aaacacaacc
     1141 tgctggttac cagagagtag ttttttactc ttcatttgcg acattggttt ttgaagataa
     1201 acctggtgaa attgtaataa ctagaaatgg aaaaccagtg aaagttgtta ctttttgggt
     1261 taagactgga actacatggg aaaaattaaa gaaagacccg caatttagaa tacctacata
     1321 tcaactacaa gacggcgatt ttattttagg ttatgataga agtgttgccc aaaagacaca
     1381 gcatattgat ttcgattctc tcaatgacac tcctgttgaa gagacatggc tcagtcccac
     1441 tgtggattac attgttggcg atgttgacga cggaaaaaca tttgaagaat acatgaaatc
     1501 ggggtatgct ccattccata ttcctcaatt ccacggaatt acattggatg ctaatggtgg
     1561 tacagttggc ggcaaagcga aaaaagcttt ttatgtggct tatgagcatg taaaatgggg
     1621 tgacgagcta gtaactaaaa aaatcgttga tccatccaga gatggttata cttttgttcg
     1681 ttggcaaaca gctacaggag gaggaatacc tacttcggga caaattgaaa cgattgaatt
     1741 tactgctgta tggttaaaac atccaacaga agctgtaaaa gtttttaacc cagaacaact
     1801 gagtgagact gataaaacaa aagttgcaga cctgattcgt agtgtcaatc ctgacttaca
     1861 gaatagcatt caagaaataa ctgtagcgaa taatggtgat gctacggtta gatttacaac
     1921 gggtgacgag cggactattt caggagcgag tttggtatcg gacaactctg cagaacaacg
     1981 cgctaaagct gaggaatcta tctccgatgc agtattgaaa aaacaggatg atattgcaaa
     2041 aagtcagcta acggcaaaag agcaacaaga taagttagct gaattgacaa aacttcaaga
     2101 atctatagaa gagcagattg agggagcgac ctctgaagat gctcttgata agatcgttaa
     2161 agatggtaag aatagcattg aaaactttga tttaacgagt gctactaaga aagtagccgc
     2221 taaggaagct cttgaagaag cctacaatgc taagaaagat gagattgata actcgtcctt
     2281 gaccgcagaa gaaaaagccg caaaacaagc agaattggat aaagccaaag aggatgcaga
     2341 taaagcaatt gatacagcaa ctgatgatgc aggcgtagcg caagcactaa cagacggcaa
     2401 aacagccatt gctggaatcg acataagcac aagtgctaag aaggctcaag ctaagcaaga
     2461 cttggaaaca gcctataacg ctaagaaaga tgagattgac aactcatcct tgaccgcaga
     2521 agaaaaagtc gcaaaacaag cagaattgga taaagctaaa gctgacgcag ataaagcaat
     2581 tgatgcagca actgatgatg caggcgtagc gaaagctctt gaagctggta aagcaacgat
     2641 tgacgcaatc aatacaacta caagtgctga gaaagcagcc gctaaggaag ctcttgaaga
     2701 agcctacaac gctaagaaag cagaaattga aaattctgcc cttacagc
//
LOCUS       DATUCG010000194         2661 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.197, whole genome shotgun sequence.
ACCESSION   DATUCG010000194 DATUCG010000000
VERSION     DATUCG010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.197"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            107..1051
                     /locus_tag="VCA20_002325"
     CDS             107..1051
                     /locus_tag="VCA20_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEP1836045.1"
                     /translation="MKQIFLSTMTDLEEIQTFGPGAWINLVNPSQSESMEVAEHYKID
                     IADLRAPLDAEESSRIAVEDDYTLILVDVPILEERNNKTYYITIPLGIILTDDVIITT
                     CLEPLPLFEQFIHRRLRNFFTFMKSRFVFQILYRNAQLYLSALRTIDRKSEEIEKQLH
                     EATRNEELIVLMELEKTIVYFKASLKTNERLVKKLASSSRLLRKYEEDEDLLEDTLVE
                     TQQAIEMADIYGSILNSMTHAFASIISNNQNTIMKTLALVTIVLSIPTMVFSAYGMNF
                     KDNIIPFNDIPYAFWWIILFAFAISLSLTFYFIRKRWF"
     gene            1067..1723
                     /locus_tag="VCA20_002326"
     CDS             1067..1723
                     /locus_tag="VCA20_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEP1836046.1"
                     /translation="MSFTEINGLTKKNQEFIHIATNQLIKDGKSDSEIKELLEEILPT
                     IIEKQKTGVTARNIYGAPSEWAASKTISEQEKKDQVEYNENPWLMWLDSSLFMLAIIA
                     GINGLMNLFDQGAQYGLLTLFVIGFGVGAGMYLMYHFVYREQIKTGQRPKLLKAIAFL
                     GLATLAWSVVFILAALIPAAFNPVLPPLVTILIGAAAFGARYLLKKKYNIRNAMSPVQ
                     "
     gene            complement(1950..2639)
                     /locus_tag="VCA20_002327"
     CDS             complement(1950..2639)
                     /locus_tag="VCA20_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HEP1836047.1"
                     /translation="MSHLQYTAKSHHLQWNVRQLSQICHHFYQNYCPDSFKHQRNVGL
                     VKVSDESILVLLLLQAELGITSQRHFYTICHLFPCGQLLERSRFNRRSKQLIWLVQLI
                     RKAMSEMLPSDKLAIIDSFPLPLCQPVRNHRVTIFKGLADIGYNGSKHLWFYGFKVHM
                     LVTLSGYILNYVVTPASVHDIKVVDELLEGCRQSVVLADLGYLNQELKDRLEQKGYHL
                     WTPLRQNMVGA"
BASE COUNT          810 a          537 c          524 g          790 t
ORIGIN      
        1 tctacatatt atatcacatt tctatataaa acctttccga aactgcacat tttttggtat
       61 aatagaggtt gcgttaacgc aacaaagata aaaggagggc aaagatatga aacaaatttt
      121 tctatcaacc atgactgatt tagaagaaat ccaaaccttt ggaccagggg cttggattaa
      181 tttggtcaac ccctcccaga gcgagtctat ggaagtggct gagcactata aaattgacat
      241 cgctgacctg cgagcaccac tcgatgcgga agaatcctct cgtatcgccg tcgaagatga
      301 ctatactctc atcctggtcg acgtcccaat tcttgaagaa cgaaacaaca agacatatta
      361 tattactatt cctttgggaa ttattctaac agatgatgtg attatcacga cttgtttgga
      421 accattgcca ctgtttgagc agtttatcca tcgtcgtcta cggaatttct tcactttcat
      481 gaagtctcgt ttcgttttcc aaattctcta ccgcaatgct caactctact tgtcagccct
      541 acggaccatt gacagaaaga gcgaagagat tgagaagcaa ctccatgagg ccactcgaaa
      601 tgaagaatta attgtactca tggaattgga aaaaaccatc gtctacttca aagcttccct
      661 caaaaccaat gagcgtttgg tcaaaaaact tgctagttcc tcacgtctac tccgtaagta
      721 tgaagaggat gaggatttgc ttgaagatac cttggtcgaa acccaacagg ccattgagat
      781 ggcagacatc tatggttcca tcctgaactc tatgacccat gcctttgctt ctatcatttc
      841 caacaaccaa aacaccatca tgaaaacctt ggcattagtg acaatcgttc tctctatccc
      901 aaccatggtc ttctctgcat acggtatgaa ctttaaggat aacatcattc cttttaacga
      961 tattccctat gctttttggt ggattatcct cttcgcattt gcaatcagcc tatcactcac
     1021 cttctacttc atccgtaaga gatggtttta acctaaggag attaacatgt catttacaga
     1081 aatcaacgga ttaacaaaaa agaaccaaga atttatccat attgcgacca accaactgat
     1141 taaagatgga aagtctgata gtgaaatcaa agaactcttg gaagaaattt taccaactat
     1201 cattgaaaaa caaaaaactg gcgtgactgc ccgcaatatc tatggagcac caagtgagtg
     1261 ggctgctagt aagacaattt ctgaacagga aaagaaggac caagtggaat acaatgaaaa
     1321 tccatggttg atgtggctag attctagtct tttcatgctt gcaattattg ctggtatcaa
     1381 tggtttgatg aatctttttg atcaaggtgc acagtatggg ctattaacct tatttgtcat
     1441 cggtttcggt gttggtgcag ggatgtactt gatgtaccat ttcgtttaca gagaacaaat
     1501 taaaactggg caacgtccta aattgctgaa agctatcgct tttcttggac ttgctacgct
     1561 ggcttggtca gtcgttttca ttctagctgc actgattccg gccgcattta acccagttct
     1621 acctccgctt gtgactattc ttatcggagc tgctgctttc ggtgcacgct acctcttgaa
     1681 aaagaaatat aacattcgca atgccatgtc cccagttcaa taatatagtt aaatgaaaca
     1741 aaaaacagta catttatgat ataatacccg tggtgctagt tcatttcaaa atagcgtaag
     1801 ttatgagcta gaataatttg ttccattcgt aactgtagtc cggatatccc tctagctaat
     1861 gtatgttcga tgtcaaaaag gcgacacagt tcagaaaaac gagtttcaat agttcgtctc
     1921 atgacaagta actgccagtg attatgttgt tatgcaccga ccatgttttg acgtaatggt
     1981 gtccataagt gatagccttt ctgttctaac ctatctttta gctcttgatt gaggtagcct
     2041 aaatcggcaa gaactacaga ttgcctacag ccttctagga gttcatcaac gactttaata
     2101 tcgtgaacag aggctggtgt cacaacatag ttcaaaatat aaccagataa ggtcactagc
     2161 atatgaactt tgaagccgta gaaccaaaga tgtttagagc cgttgtagcc aatatcggct
     2221 aatcctttaa aaatggtaac tctgtgatta cgaacgggtt ggcaaagtgg caaaggaaaa
     2281 ctatctataa tggctagttt atcagatgga agcatttcac tcatagcttt tcggattaat
     2341 tggactagcc aaatcaattg tttagaccga cgattgaaac ggcttctttc tagtaactga
     2401 ccgcaaggga agaggtggca gatggtgtag aaatggcgct gtgatgttat gcctagttct
     2461 gcttgtaaca aaagtaagac aaggattgat tcatctgaaa ctttaactaa gccaacatta
     2521 cgttgatgtt tgaaggagtc tggacaatag ttttggtaga agtgatgaca aatttgtgat
     2581 aattggcgta cattccattg caaatgatga gatttagcgg tatactgtaa gtggctcatt
     2641 tggactaacc tcctgtatgt t
//
LOCUS       DATUCG010000195         2623 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.198, whole genome shotgun sequence.
ACCESSION   DATUCG010000195 DATUCG010000000
VERSION     DATUCG010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.198"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(291..2558)
                     /gene="glgP"
                     /locus_tag="VCA20_002328"
     CDS             complement(291..2558)
                     /gene="glgP"
                     /locus_tag="VCA20_002328"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HEP1836048.1"
                     /translation="MINLQNFVQSMYAKRIEDCTDQELYYALLAFTKQQSEAKYTNDQ
                     KKKVYYISAEFLIGKLLSNNLINLGLYDEVKSQLVAAGKDLIAIEDMELEPSLGNGGL
                     GRLAACFLDSIASLGLNGDGVGLNYHFGLFRQLFEYHQQSAVPNEWITPRSWLTESPI
                     SYQVPFANFTLTSKLYDIDVSGYKTERKNRLRLFDLGSVDDNIIYDGIEFDKEDIFRN
                     LTLFLYPDDSTDEGKVLRIFQQYFMVSNAAQLLIDEAVAKGSNLHDLPDYAVVQINDT
                     HPSLVIPELIRLLELRGISFDEAVEIVKKMTAYTNHTILSEALEKWPLDFLNYVVPHL
                     VPFIVELDRRAKEVKDDPAVNIIDEYGRVHMAHMDIHYGYSINGVAALHTEILKTSEL
                     KAFYELYPEKFNNKTNGITFRRWLMHANPSLASYLDGLLGHGYHHDASELEKLLDYKN
                     DKELKSWLEKTKQHNKRKLQRHIAKTQGVHVNPESIFDVQIKRMHEYKRQQMNVLYVI
                     HKYLDIKAGNIPTRPLTVFFGGKAAPAYTTAQDIIHLILVLSQVIKNDPEVAPHLQLV
                     MIENYNVTEASFIIPAADISEQISLASKEASGTGNMKFMLNGALTIGTDDGANVEIHE
                     LVGDENIYIFGQDSQTVIDHYANGTYHPYSFYEREAIRPLIDFITSDTTRHAGGIDER
                     LWRLQDNLKHNDHFMTLLDLEDYISTKEQMLADYEDRDSWLDKVIVNIAKAGFFSSDR
                     TIQQYNEDIWHLEAK"
BASE COUNT          784 a          549 c          511 g          779 t
ORIGIN      
        1 cttacaaata gggcttactt aacaactact tgaagttttt agaggtgccc ataaagaatt
       61 tcaattttga agtttttgaa aagagaatgg ccagtcaaaa tctatgattt ccaatctgaa
      121 cttagatatt gaaattttac acatagaaat tgacctcaat atgctacttt tgatttttat
      181 tgagtagaaa cctttagtta gcccttggaa atctcctagt acaaaccaca acaactcata
      241 ctttcaatgt cagaacacaa aaaatgaccc cgcaaggtca tttttaattt ctatttcgct
      301 tccaaatgcc agatgtcttc gttatactgt tgaatagtac ggtctgatga gaagaatcct
      361 gctttagcaa tgttgacgat aaccttatcc aaccagctgt cgcgatcttc gtagtctgct
      421 aacatctgct ctttagtcga gatataatct tccaagtcaa gcaaggtcat gaaatggtca
      481 ttgtgcttaa ggttatcctg taagcgccag aggcgttcat cgataccacc agcatgacga
      541 gtggtgtcag aagtgatgaa atcaatcaat ggacggatgg cttcacgttc gtagaaggaa
      601 tatggatggt aggtaccatt tgcatagtgg tcgataacgg tttggctatc ttgaccaaag
      661 atataaatat tttcatcacc taccaactca tgaatttcca cattggcccc atcatctgta
      721 ccgatggtca aggctccgtt cagcatgaat ttcatgttac ctgtaccaga tgcttcctta
      781 gatgcaagtg aaatttgctc agaaatatcc gctgctggaa tgataaagct agcttcggtt
      841 acgttgtagt tctcaatcat aactagctgc aaatgtggtg ccacttctgg atcatttttg
      901 ataacctgtg ataagaccag aatcaagtgg atgatgtctt gagctgttgt ataggctgga
      961 gctgctttcc ctccaaagaa gactgttaac gggcgagtcg ggatattgcc cgccttgata
     1021 tccaaatact tatggataac atagaggaca ttcatctgtt gacgcttgta ctcgtgcatg
     1081 cgcttgattt gtacatcaaa gatggattca ggattgacat gtacaccttg agttttggca
     1141 atatgacgtt gcaacttccg cttgttgtgt tgctttgtct tttccaacca agatttcaat
     1201 tccttatcat ttttgtaatc caacagcttt tcgagttcac ttgcatcgtg atggtagcca
     1261 tgtccaagta ggccgtccag gtagcttgcc aaacttggat tagcatgcat gagccagcga
     1321 cggaaggtaa tcccatttgt cttattattg aatttttcag gatatagctc atagaaagct
     1381 ttcaactctg aagttttcaa aatctctgta tgaagtgccg ccaccccatt gatagaataa
     1441 ccataatgaa tatccatgtg tgccatgtga acacggccat attcatcgat gatattgaca
     1501 gcaggatcat ccttcacttc tttggcacga cgatccaatt ctacgatgaa cggtaccaag
     1561 tgcggaacca catagttcag aaaatcaagc ggccattttt ccagcgcctc tgacaagatg
     1621 gtatggttgg tataagcagt cattttctta acaatttcaa ctgcctcatc aaacgaaata
     1681 ccacgaagct ccaaaaggcg aatcaattct ggaataacaa gtgatgggtg agtatcgttg
     1741 atttgaacaa cagcataatc aggcaggtca tgtaagtttg accccttagc aactgcttcg
     1801 tcaatcaata gttgcgcagc gttagacacc atgaaatact gctggaagat gcggagaact
     1861 tttccttcat cggtcgaatc atctggatag aggaagaggg tcaagtttcg gaaaatatct
     1921 tccttatcaa attctattcc gtcataaatg atattgtcat caactgaacc aaggtcaaac
     1981 aagcgaagac gattcttacg ctcagtctta taaccagaca catcgatatc gtacagctta
     2041 gatgtcaagg taaagttagc aaatggcacc tgataagaaa tcggtgactc tgttaaccat
     2101 gaacgtggtg taatccactc gtttggcaca gcagactgct gatggtattc gaataattgt
     2161 ctaaacagtc caaagtgata attcaagccg accccatcac cgttaagacc tagactagca
     2221 attgaatcaa ggaagcaggc agccaagcgc cccaaacctc cattaccaag agacggttca
     2281 agctccatgt cttcaatagc aatcaaatcc ttaccagccg ccaccaactg gcttttaacc
     2341 tcatcataaa gaccaaggtt gattaaatta ttagacaaga gcttaccaat taagaactct
     2401 gctgaaatat agtagacctt tttcttttgg tcatttgtat acttagcttc actctgctgt
     2461 tttgtaaaag caagcaaggc ataatagagt tcttggtctg tacaatcttc aatgcgtttg
     2521 gcatacatag actgaacaaa attttgtaaa ttaatcatga gagctctcct aaatatatta
     2581 atgtaatcat tcagaaaggt ttcgctttgc ctttgtaaac gtt
//
LOCUS       DATUCG010000196         2579 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.199, whole genome shotgun sequence.
ACCESSION   DATUCG010000196 DATUCG010000000
VERSION     DATUCG010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.199"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            <1..372
                     /locus_tag="VCA20_002329"
     CDS             <1..372
                     /locus_tag="VCA20_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEP1836049.1"
                     /translation="GYAYGQCTWGVAARINQLGLKLKGKNGEKIPIISTMGNGQDWVR
                     TAASLGGETGTSPQAGAILSFAGGGHGTPTEYGHVAFVEKVYPDGSFLISETNYNGNP
                     NYTFRKLSGVDSSLSFAYTTK"
     gene            438..1544
                     /locus_tag="VCA20_002330"
     CDS             438..1544
                     /locus_tag="VCA20_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEP1836050.1"
                     /translation="MIQRLNVNTEVLSTYIRNSNTPIEYLRQKLPNIDKFLKGELEPT
                     FNQISALSKMINVPTGLLLLKEPISPSSLNVDFRTFNSQLLSDISSELKDTIIEMQEK
                     QEFLKEIVENECPFVGIFTFDDDYSDVILKAREFLGNEITRGRFNNYRKILGQLGIFI
                     FLNGKYKDNTHRPLNLNEFRGFVLSDKKAPIIFINQLDSKTGQLFTLIHEFVHLLYGD
                     SDLFDGEDPKKRSKTEAIINSITAEILVPKELISDMFDNQIDIRTSLDNLSRKFEVSK
                     FVVLRRLYDLSMVSRKVFISLQRELEAEFKSKEKDKKKSGGGNYQNNLRFRMDSNFFN
                     HVNNAVQQNKITYTDAFGIVGVGYKGYKILSRGG"
     gene            1548..2048
                     /locus_tag="VCA20_002331"
     CDS             1548..2048
                     /locus_tag="VCA20_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4411 family protein"
                     /protein_id="HEP1836051.1"
                     /translation="MYLLDSNIYINFYDRYYRKDFFPTFWSNFAGILNSSVVIPKIVV
                     SENYQDEWFRDWLSNNYTKSIIDHKSYVVEWRAIIQYIQSSPYYKDTALMSQKGWAND
                     DIADPWLIAIAKSMEYTIVTDEVRNVNLNSANPSKNAKIPDVCDQLGVKCISMNEFFE
                     EIGLSV"
     gene            2353..>2579
                     /locus_tag="VCA20_002332"
     CDS             2353..>2579
                     /locus_tag="VCA20_002332"
                     /inference="COORDINATES: protein motif:HMM:TIGR03726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1836052.1"
                     /translation="MTKTCNHHFLVNQEKGEKHVFRKSKKYRTLCSVALGTMVTAVVA
                     WGGTVASADEVTTSVDTTIQRTENPATNLPEA"
BASE COUNT          899 a          364 c          487 g          829 t
ORIGIN      
        1 ggatatgcct acggccaatg tacttgggga gttgcagccc gtatcaacca actgggtcta
       61 aaactcaaag gtaaaaacgg tgagaagatt ccaattatca gtaccatggg caatggccaa
      121 gattgggtac gaacagccgc aagtctcggt ggagagaccg ggacaagtcc gcaagcagga
      181 gctattcttt cctttgcggg aggaggacat ggcacaccaa cagaatacgg tcatgtggct
      241 tttgtggaga aagtctaccc agatggctca ttccttatct ccgaaacaaa ctacaatggc
      301 aatccaaact ataccttccg taaattatct ggagtggata gtagcttgag ttttgcttat
      361 acgacgaaat aaaaaaataa tatagaattt ttctaaattt tttggtaaaa taaattaaaa
      421 agtatgggag gcagtttatg attcaacgtt taaatgtaaa tactgaagtt ctatctacat
      481 atattcgtaa ctctaatact cctatcgaat atttacgaca aaaattacct aatattgata
      541 agtttttaaa aggggaatta gagcctacct ttaatcaaat atctgcactt tctaaaatga
      601 taaatgttcc tactggatta ttgttattaa aagagccgat ttctcctagc tctttgaatg
      661 ttgattttag gacattcaat tcacaacttt tatctgatat cagttctgaa ttaaaagata
      721 caattattga aatgcaagag aaacaagaat ttttgaaaga aatagttgag aatgaatgtc
      781 cttttgtagg tatttttact tttgatgatg attactcaga tgtaattttg aaagcaagag
      841 aatttttagg gaatgaaatt accagaggaa gatttaataa ttatagaaaa atactcggtc
      901 aattaggaat tttcatattt ttaaatggta agtataagga taatacacat cgtccgttaa
      961 atctaaatga gtttagaggt ttcgtgttat ctgataagaa ggctccgatt atttttatta
     1021 atcagttgga ttcgaagact ggacagttat tcacattaat tcatgagttt gtccatttat
     1081 tatatgggga tagtgatcta tttgatggtg aagatccaaa aaaacgaagt aagacagaag
     1141 caataataaa ttcaataaca gcggagattt tagttccaaa agaattaatt agtgatatgt
     1201 ttgacaatca aatcgatatc agaaccagtc ttgataattt atctagaaaa tttgaagtaa
     1261 gtaagttcgt tgttttaaga cgactatatg atttatcaat ggtttcaaga aaggtcttta
     1321 tatctttaca aagagaactt gaagctgaat ttaaatcaaa agaaaaagat aaaaaaaaat
     1381 ctggtggagg taattatcag aataaccttc gattcagaat ggattctaat tttttcaatc
     1441 atgtgaataa tgctgttcaa caaaataaaa ttacttatac agatgctttt ggtatcgtag
     1501 gggttggata taaaggatat aaaatattaa gcaggggagg atgataaatg tatttattag
     1561 attcaaatat atacataaac ttttatgata gatattatcg taaagatttt tttcctacct
     1621 tctggtcaaa ttttgctggt attttaaact caagtgtggt gattcctaaa attgtagtct
     1681 cggaaaatta ccaagatgag tggtttagag attggttatc gaataactac actaagtcca
     1741 ttattgatca taagtcttat gtggtagagt ggagggccat tattcagtat atccagagta
     1801 gtccttacta taaagataca gcacttatgt cacaaaaagg atgggcaaat gatgatattg
     1861 cggacccatg gttgatagct attgcgaaaa gtatggaata tactatagtt actgatgaag
     1921 taagaaatgt gaatttaaat tctgcaaacc ctagtaaaaa tgctaaaatt ccagatgtgt
     1981 gtgaccaatt aggagtaaaa tgtatctcaa tgaatgagtt ctttgaggaa attggtttat
     2041 ctgtttaaaa agtctccttt tatgagtagg gtactgaaaa gtacttctta taaaaaaatc
     2101 ataaataata gaaattattt tatctaatat ataacttatt aacgaatgat tgaagagtgg
     2161 tttaataact acgcatcatc attcgttttt tactgcaatc attgttgcta ctcggacttc
     2221 ttaagttacc tctttatggg tgactttttc ttttccctga actgattgtg ataacaaaat
     2281 caagccttct tttttatgat gaggaaagag tgacagtttg ttgctacata aaaaagaaaa
     2341 gaaggactat ttatgaccaa aacatgtaat catcactttc ttgtcaatca ggaaaaaggc
     2401 gagaaacacg tctttcgcaa gagtaaaaaa tatcgtactt tatgttccgt tgcccttgga
     2461 accatggtga cggctgttgt tgcttgggga ggaacggttg catctgctga tgaagttaca
     2521 acttcagttg acaccaccat tcaacgaacg gagaatccag ccacgaattt accagaagc
//
LOCUS       DATUCG010000197         2562 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.200, whole genome shotgun sequence.
ACCESSION   DATUCG010000197 DATUCG010000000
VERSION     DATUCG010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.200"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(876..1736)
                     /locus_tag="VCA20_002333"
     CDS             complement(876..1736)
                     /locus_tag="VCA20_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP1836053.1"
                     /translation="MTLLASRLRSRRLELNLSQAELAEGVCEQGQISRIEKGKYNPGS
                     DLLYKLSLKLNVSMNYFFDESASKESSNIENFKILSKKLLTGREYESLKYLYELELGK
                     KQTLPLSDQLYLTWIESIILFHCDNWKTEAIKKLESVINKMTENDQEYFKYLNSLLNF
                     YSLMGNKEISEKMYLTIISKMDSSIITDIEDLELLIKVRYNYCRYLWLENKIDIAITE
                     ILETIDICNTNNHCYLLADLYCLLGNVSEGFLEKVEVRRYFSYAQFLYNLYGNERMSL
                     TLENYIKKNF"
     gene            complement(1978..2217)
                     /locus_tag="VCA20_002334"
                     /pseudo
     CDS             complement(1978..2217)
                     /locus_tag="VCA20_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042188.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
     gene            complement(2242..2535)
                     /locus_tag="VCA20_002335"
     CDS             complement(2242..2535)
                     /locus_tag="VCA20_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009567943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="HEP1836054.1"
                     /translation="MAEEEIKQLQNKRKKLISQQKQEERKKRDRRLYEKGAVFESIFT
                     ESKDFTKDEFYQLIIFPNIEEAVNQKIRKIIEKREERENQSTENQEEERSIEE"
BASE COUNT          775 a          447 c          321 g         1019 t
ORIGIN      
        1 agttaccgaa caagtctact tcatcagcca ttgtttttcc ctcctttcta tacattttat
       61 aagtacttat acaaaataat aattactttc actcactaca agactattta ttaagtaaag
      121 taatttataa ttgacttaac taactgatta ggtaatcaac ttttttccaa ataattcata
      181 ttttttacaa tctatttgat tatatgaacc acgcctggat ctataccttg actacatgag
      241 gtaatatttg ccgttcctat agcaataatt gacaagacta aaataattaa actgaatttg
      301 ttgctctttt tcatttttgt aacccctttc tttgaaatca atgatatcac tttaataaat
      361 attcgtcaac cataaaaatg tttattttat ttttttaaaa aaaaatacac tttggttata
      421 caaaaagata tccatttgtg aaaactgttt gctaaaccaa ataaaaatct ttacaactat
      481 taatctcgtt acaacatcga ataatgggat gaaatgaaaa atatctcacc aatccaaatc
      541 atgaacaagc tatttttcaa atagtttttt ggggagagtt agttttcttt tagcatttct
      601 atccaaattc tataagagat tgcttttatt cacaaccaga tactaaaata tttttgagga
      661 actattttgt tactgatatc tacatccact tattcgattg ccaataactt caaaattacg
      721 aaacggcctt gcacctcttt attcgaggaa caaggtcgtt tcgtaatttt ttaacatttc
      781 attatcacct atttagctag aagttatttt aaagagtcaa aatgtttaca caatacccaa
      841 aaacatacta attgaattta ttcaagaaat attatttaaa aatttttctt aatatagttt
      901 tctaaagtta gggacattct ttcgtttcca tataggttgt aaagaaactg agcgtacgaa
      961 aaataccttc ttacttccac tttttctaaa aagccttcac ttacatttcc taacaagcag
     1021 tacaaatcag ccaataaata gcaatgattg ttggtattgc aaatatcaat tgtttctaaa
     1081 atttcagtta ttgcgatatc aattttattc tctaaccata gatatctaca gtaattatac
     1141 cttactttaa tcaacaattc taaatcctca atatcagtta ttatggaact atccattttg
     1201 gaaattattg taaggtacat tttttcacta atttctttat tccccattag tgaataaaaa
     1261 tttagaaggc tatttaaata tttaaaatac tcctgatcat tttctgtcat cttgtttatt
     1321 acagattcaa gcttcttaat tgcctctgtt ttccaattat cacagtgaaa aagaataatt
     1381 gactcaatcc atgtcaggta aagctggtca gaaagaggaa gagtttgctt ttttcctagt
     1441 tctagctcgt ataggtactt tagcgattca tattctcttc ctgtaagtaa cttttttgac
     1501 aaaattttaa aattttcaat attactagat tcttttgaag cgctttcatc aaagaagtaa
     1561 ttcattgata cattaagctt taatgataat ttataaagta ggtccgatcc tggattatat
     1621 tttccttttt cgattcgact aatttgtccc tgttcacaga ctccttcagc aagttctgct
     1681 tgtgacagat ttaattctaa cctccgactt cttaatcggc ttgccaataa tgtcattatt
     1741 tctcctctca aagcatttac attgcaggag caattttatc attattttga ctattattca
     1801 agaaaaaata attatataaa ttttgtagaa tatttttgta atattggatg acagctaatt
     1861 tcttcccaga taattaaaat agaatagggt agggtaggaa agacgtccat gttggctcag
     1921 cactcgtcaa cagattaatg aaaactaaca taaagcactc atgacacata gatatctatt
     1981 ttgtgtttct attttttctt cttttccaat tccccttatc caatttagta atgcctttat
     2041 tcgtcttgat atttctctta aaatcatatt ttgatgtttg atttctcggt taatatttcc
     2101 tctgtctgtg gctatgcctt tcttctccat ttgggttgcg gatacgccta aatgaatggt
     2161 cggtatttgt tctatgcact gtctttgata agaacgatgg tctacttttt cctgtatatg
     2221 tgcgcccttg taaacaaggg actattcctc tatgctcctt tcttcctctt ggttttctgt
     2281 actttgattt tctctttcct ctcgcttctc tatgattttt ctgattttct gattgacagc
     2341 ctcttcgata ttcgggaata tgattagctg ataaaattca tctttggtga agtccttgct
     2401 ttctgtaaag atactttcaa agactgctcc tttctcataa agccgtctat ctctcttttt
     2461 tctttcttct tgtttctgtt gactgatgag tttttttctt ttgttttgta gttgcttgat
     2521 ttcttcttct gccattaaaa ttttttcatc tatagttaaa tg
//
LOCUS       DATUCG010000198         2520 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.201, whole genome shotgun sequence.
ACCESSION   DATUCG010000198 DATUCG010000000
VERSION     DATUCG010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.201"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            359..1183
                     /locus_tag="VCA20_002336"
     CDS             359..1183
                     /locus_tag="VCA20_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1836055.1"
                     /translation="MITVQHIHKQFGDKVALDNISFDIQEGQIFGFLGPSGSGKTTMI
                     KIITGQLPADSGKSYLLGKESTLLSPEDFEAMGLVSDSSGFFEKMTLENNLLFSAKFF
                     GVSSKAVEVILQKVGLLDSKKTIAEKLSTGMKQRMLLARALINSPKVLFLDEPTSGLD
                     PTTSKQIHELLLELKGKGTTIFLTTHDMAEATFLCDQLVLLNQGNLVEKGSPQDLIHK
                     YNVTKKVRVTFEDGSDNELLFSELTPEKLAQATTIHSCEPTLEDIFIQQTGATLNV"
     gene            1176..1919
                     /locus_tag="VCA20_002337"
     CDS             1176..1919
                     /locus_tag="VCA20_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP1836056.1"
                     /translation="MYKRFLALLWLRTQVLVTNKNILLPVLMPYFMLLLFKFVMKTDD
                     TKAMDLMGICFSMGIGMSVGSPISAMISEEKEKNNLSTLLLTGVRPSEYVLSILCYPV
                     VLSFVNLILFPAITQADVSGYVLSYSVIMLLTSLASILINLLIGATSQSQSKSQVTSM
                     LITMAVSMGPSLSLMNETVGQILKYSFLGAYIEFFKNPHFELLNQEFYYLLGWIVLIS
                     LALILYLGNWMKYFRNSLSGMIEKIQYSL"
     gene            2239..2502
                     /locus_tag="VCA20_002338"
                     /pseudo
     CDS             2239..2502
                     /locus_tag="VCA20_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637270.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid transporter"
BASE COUNT          806 a          375 c          531 g          808 t
ORIGIN      
        1 gcgaataaat aggtgaaggg gaaaacaagc tttctccgct atacaatcat tcgtgagtaa
       61 aaataaatta ttttttacaa aagtttacga atagataagt gtaggggaag caagctcaca
      121 acttattcaa aaaaataaga ttttttcaaa aaatgaaaac tatttgcgaa taagtaagtg
      181 agagggaaaa ttcccctcaa ggagatacac acttgaaaaa aataaatcat tttccaaatg
      241 atttacgaat aaacaagtgt aggaaaaatg aaaggagaag aaggatgatt atagcaccag
      301 ctttatggat tttatcatgg tggaaaaaat aagtttgttt taatttaggg gagtagagat
      361 gataacagtt caacatatcc ataaacagtt tggagacaag gttgccttag acaacattag
      421 ctttgacatt caagaaggac aaatctttgg attcttgggt ccctctggct cgggaaagac
      481 aaccatgatt aagattatta ccggacagct accagcagat agcggaaaat cttatctact
      541 aggtaaagaa tcgaccctat tgtcaccaga ggactttgaa gcaatgggct tggtcagtga
      601 ttcaagtggc ttcttcgaga agatgacttt ggaaaataat ttgttgttct ctgcaaaatt
      661 ctttggtgta agttcgaaag cagtagaggt tattctacaa aaagtagggt tgctggatag
      721 taaaaagaca attgcagaaa aactatctac aggcatgaag caacgaatgt tgcttgcaag
      781 agccttaatc aattcaccaa aagtattgtt tcttgatgag ccgacaagcg gtctagatcc
      841 tacaacttca aagcagattc atgaactctt attggagcta aagggtaagg ggacgacgat
      901 cttcttgacc acgcatgata tggcagaagc gacattctta tgtgatcaat tggttctatt
      961 gaatcaagga aatttggttg aaaagggaag tccgcaggat ttgattcata agtacaatgt
     1021 cactaaaaaa gtgagggtta cttttgaaga tggtagtgat aatgagctat tgtttagtga
     1081 gttgacacct gaaaagctgg ctcaggcaac aactatccac tcttgtgaac cgactttgga
     1141 agatattttt attcaacaga caggagcaac cttaaatgta taaacgattt ttagccttgc
     1201 tttggcttcg cacacaagtc ttggtgacca ataaaaacat cctattgcca gtattgatgc
     1261 cttacttcat gttattgctg tttaagttcg tcatgaaaac agatgatacc aaggcaatgg
     1321 atttgatggg aatttgtttt tcaatgggga ttgggatgtc agtaggaagt cctatatctg
     1381 caatgatttc tgaagaaaag gaaaagaata atctgtctac cttgttactg acaggtgttc
     1441 gtccaagtga atatgtactg tctattctgt gttaccctgt tgtccttagc tttgtcaacc
     1501 ttattctatt tccagctatc acacaggctg atgtatcagg atatgttctt agctattcgg
     1561 ttatcatgct tttgacatca ctggcttcca ttctaatcaa ccttttaatc ggggcaactt
     1621 cccaatctca atccaagtca caggttacct cgatgcttat cactatggca gtatctatgg
     1681 gacctagcct gtctttgatg aatgagacag tggggcagat tttgaaatat agttttctag
     1741 gagcttatat tgaatttttc aaaaatcccc attttgaact tttgaatcaa gaattctatt
     1801 acctattggg gtggattgtc ctgattagtt tggccttgat tctctacttg ggaaattgga
     1861 tgaaatattt taggaactcc ctatcaggaa tgatagaaaa aattcaatat tctttataat
     1921 atgagtaaat tagaaaggag aaatttatgg tatcagcatt cattatcggt gtcggaatta
     1981 ttgtttattg tattcaaaac taataggaaa ttgaagaaag agtattatat tatgaaggag
     2041 gagaaatata tgctagtagc tgcagttaca ggtttcgttg ttggttatgt ggcggcacat
     2101 tatttgagac cataattgat ggtgaaatat gagtattaaa attctttggt ttttccagag
     2161 aatttttctt tttgtttacg aataaatagg tgagggagtt attgctcatc tatcttattg
     2221 ataaaaaggg gaaacaatat gaatatgttt aggtcaattt ttaagtcggt aatacatcga
     2281 agggatgttg ctttgttcta tgcttttgcg ggtttgccaa tcttagttcc tatattgtcc
     2341 aaatttttgg taggcgtaaa ggctgaatat acggataatt ttttagattt tctaggagca
     2401 gcattggcaa cacaggacgg gatagtgtta ccaacattgc tgttatccgt gataattttc
     2461 gcagttttta gggatgagat tgatagcggt attctatttt tatagttaaa tgaaacaaaa
//
LOCUS       DATUCG010000199         2412 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.202, whole genome shotgun sequence.
ACCESSION   DATUCG010000199 DATUCG010000000
VERSION     DATUCG010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.202"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(<1..190)
                     /locus_tag="VCA20_002339"
     CDS             complement(<1..190)
                     /locus_tag="VCA20_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HEP1836057.1"
                     /translation="MSYLQYTAKSHHLQWSVRQLSQICHHFYQNYCPDSFKYRRNVGL
                     VKVSDESILVLLLLQAELG"
     gene            474..1175
                     /locus_tag="VCA20_002340"
     CDS             474..1175
                     /locus_tag="VCA20_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEP1836058.1"
                     /translation="MWSPKKLTVLKWFDILILTIILFGDGIINSTLQYIALQNQTTTL
                     QENLTFSPMDNYKALALQLVWLTIAIFYLLLRNFDFSIWKKHIFITPWVPLQAVALFI
                     FSALCLDIYHLVSYQFLASNTPSMFQLFPNIDLSLILYSLLNGFYEEIFFLNLCLLVN
                     PKYVKWAFLYSLIIRCSFHTYQGLISALGLGLILGTIFYLLYQKIKPKNLSPFFLAHA
                     VADVIGLTVLSYILY"
     gene            complement(1415..1774)
                     /locus_tag="VCA20_002341"
     CDS             complement(1415..1774)
                     /locus_tag="VCA20_002341"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1836059.1"
                     /translation="MTFKFRDWAFYESIGGYYFIIPDGLKLTKLVNHSNTDILNHYKS
                     EGAGGDEYRAFENGKGEFDAQWGRSAGFMDNAIFNKWKEENRFSKILFRDYRRDVQTA
                     TYKLELVNLYAEMRYEH"
     gene            complement(1771..2169)
                     /locus_tag="VCA20_002342"
     CDS             complement(1771..2169)
                     /locus_tag="VCA20_002342"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1836060.1"
                     /translation="MTIESNQTLEEQPLTVEEQATKGAENEENQDDEVPNQSANLEEI
                     ENVISEPKAEATPETKVEDSTLSEFTPCAVRSAENNLDMESLPVTPELPNEKQPAYIQ
                     DTNYLGKPFQIQQSVSIEATSVTDKKLSGK"
BASE COUNT          715 a          468 c          373 g          856 t
ORIGIN      
        1 tgcctagttc tgcttgtaac aaaagtaaga caaggattga ttcatctgat actttaacta
       61 agccaacatt tcgccgatat ttaaaggaat ctgggcaata gttttggtag aagtgatgac
      121 aaatttgaga taattggcgt acactccatt gcaaatgatg agatttagca gtatactgta
      181 agtagctcat ttggactaac ctcctgtatg tttcgtcact tacagtatag tccatttggg
      241 ctttttattg tattttgaaa tgaactagca ccacgggtta ttatacttca attatatacc
      301 gattcgtaca atggtgcaac tctatcatag ataaatagag caccatgttc tgctatttca
      361 gctttctttt gacttctttt gtttaaaaca gataaaaaac agatgaaacc aaggacatat
      421 atagattggt ttgataaact gaactaaaca aaaatgaaag gagctatccc attatgtgga
      481 gtccaaaaaa attaaccgta ttaaaatggt ttgatattct cattctaaca attattttat
      541 ttggagatgg aatcatcaac tctactctcc aatacatagc cttacaaaat caaactacaa
      601 ctcttcaaga aaatttgaca ttttcaccaa tggataatta taaggctttg gctctacagt
      661 tagtttggct cacaatagcc atcttctatc tattactccg aaattttgat ttctcaattt
      721 ggaaaaaaca tatttttatt actccttggg ttccattaca agctgttgcc ttattcattt
      781 tttctgcatt atgcttggat atctatcatc tagtatccta tcagtttcta gcttccaaca
      841 ctccatccat gtttcaacta tttcctaaca ttgatttatc actgattttg tactcattgt
      901 tgaacggatt ttatgaagaa attttcttcc ttaacctgtg cctactggtt aaccctaaat
      961 atgtaaaatg ggcattttta tattcgttaa ttattcgatg cagtttccat acctatcaag
     1021 gtttaattag tgcactcggt ttaggcttaa ttctaggaac tattttctat ctactttatc
     1081 aaaaaataaa acctaaaaat ttatcaccat ttttcctagc acatgctgtg gctgatgtta
     1141 tcggtttaac tgttctatca tatattctat actagttatc taacagataa tttgaaaaca
     1201 agctatcctc atatcaccaa agactgagtg gacccctact agtcatgacc acccgtgaaa
     1261 acgggtggtt tattttgtgt aatttagaga aaagggtgcc aaaaaacgca taaaatcatg
     1321 ttgctaagaa cgttgatttt atgcgtttta ctatatgacc tatctcaata tttttattga
     1381 aatttatgtt tgtgcccaac tatacttctc agaatcaatg ttcatatctc atctcagcgt
     1441 atagattgac aagctctaat ttataagtag cggtctgtac atctctgcgg tagtctcgaa
     1501 agagaatttt tgagaaacga ttttcttcct tccatttatt aaatatagca ttgtccataa
     1561 accctgcaga ccgaccccat tgcgcatcaa attctccctt tccattttca aacgccctat
     1621 actcatcccc accagcacct tctgacttat agtgatttaa aatatccgtg ttactatgat
     1681 ttactaactt agttagtttt aagccatctg gaatgataaa atagtatcca ccaattgatt
     1741 cataaaatgc ccagtctcta aatttgaaag tcacttccca ctcaattttt tatcggttac
     1801 ggaagttgct tctatcgaaa cggattgctg tatttgaaat ggtttgccta gatagttcgt
     1861 atcttggata taagccggtt gtttctcatt cggcaattct ggagtaacag ggagggattc
     1921 catatccaaa ttattttcag ctgaccttac agcacacggt gtaaattcag aaagggttga
     1981 atcctcaacc tttgtttctg gggttgcttc agcctttggc tcagaaatga cattctcgat
     2041 ttcctctagg ttagcagact ggtttggaac ttcatcgtct tgattttctt cattctcagc
     2101 tccttttgta gcttgttctt caactgtcaa tggctgttct tctaacgttt gattagattc
     2161 tattgtcaca aggcttgcat cggttgatgc ctccgtatcc gccttgatat ttgatctgat
     2221 tgtattacat tacccgttgt gctagttaat tctagttaaa taaaaacact ttgcgtatca
     2281 taaactcaaa acaaccacga aatgagggaa tgatatgcaa agccaacatt attatcttgc
     2341 aaatcccaat caaacacaag ttattttttc aaaaatcttt acccaagttc tcgctttata
     2401 tgagcgattt gt
//
LOCUS       DATUCG010000200         2291 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.203, whole genome shotgun sequence.
ACCESSION   DATUCG010000200 DATUCG010000000
VERSION     DATUCG010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.203"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            560..2218
                     /gene="ltrA"
                     /locus_tag="VCA20_002343"
     CDS             560..2218
                     /gene="ltrA"
                     /locus_tag="VCA20_002343"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019034340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEP1836061.1"
                     /translation="MNSKLCATTNIANSWESIDFVKAEIYVKKLQMRIVKAWKLGKFN
                     RVKSLQHLLTTSFYARALAVKRVTENQGKKTSGVDKELWLTPNSKYQAIKKLKVRGYC
                     PKPLRRIYIPKKNGKKRPLSIPTMTDRAMQTLFKFALEPIAETTADPNSYGFRSKRST
                     QDAIEQCFSALSKQKSAKWVLEGDIKGCFDNISHEWIMKHIPMNKTILGKWLKSGYIE
                     NRKLFPTEFGSPQGSPISPIISNMVLDGIERKLSTTFRKKKVNGKVYAPKINFVRYAD
                     DFIVTGVSKELLENEVKPAIIEFLKERGLELSEEKTLITHITDGFDFLGVNIRMYDGK
                     LLTKPSKKNYESIVSKIRDIIKNNPSMKQELLIRKLNPIIIGWVNYQKHNVSTEAFQR
                     LDFDIYQCLWQWCIRRHPKKGRKWVANKYFHTFGSRSWIFSVPTADTMENGEPFYLRL
                     KYASDTDIRRHIKIKAEANPFDEQWQLYFEERQEKQMRQELKGRRVINGLYYKQKGIC
                     PVCELKITKETDFRVHQTVKDHKPIKTLVHPTCHKNIKENTLVL"
BASE COUNT          804 a          374 c          500 g          613 t
ORIGIN      
        1 ttgaactgtc tggcatgatg accagaccaa ctattccgtt agttgagcca agtatcaact
       61 gcatgttcgc catgtggtaa gctgatacgt aaggtagccc tactttaagg tcgaaccgtg
      121 aggggagacc gatactgcta gcaacaaagc ggttagggag ctaggcttga tagtatggct
      181 aacaaatgtg aaccactgtt aaatgtcgtt aagttgaaca ggctaaagtt gctgataagc
      241 ctgaaccaaa aggtgtgcag tcggataacc ctttccatag tggggttaca cggacagatg
      301 actgccggca gagaggtgga gcctaaccta tcattttgtt cattaagtgg aacatggtaa
      361 gcctgtattg ttgcttctag caaggcaaat cgtaagaaat gctgataaca gtgcaggtat
      421 aggaaaatgg aaaaagcgaa tgccatcttg taatgagatg gatagaggtt caagatttgc
      481 ctcaactcga aagagtgcag acttccattc ggtttctaat cacaagagaa cttgtagaat
      541 ttttgaaagg agaaagcaaa tgaacagtaa attgtgtgct actactaaca tagctaacag
      601 ctgggaaagt attgactttg tgaaagctga aatctatgtt aaaaaactac aaatgcgtat
      661 tgtgaaagcg tggaagctgg gtaaatttaa ccgagtgaaa tcactccaac atttactaac
      721 aacttcattt tatgcaagag ccttggcagt gaaacgtgtc actgaaaacc aaggcaaaaa
      781 gacaagtggc gttgataaag agctatggtt aactcctaat tctaaatatc aagcaatcaa
      841 gaaattaaag gttaggggct attgtccaaa accactaaga cggatttata ttcctaagaa
      901 aaacggtaag aaaagacctc ttagtattcc aacgatgaca gatagagcaa tgcagacatt
      961 atttaagttc gctctagaac ctattgcaga aacaacagca gacccaaatt cttatggctt
     1021 tagatccaaa agaagtactc aggatgctat tgaacaatgt ttctcagctt tgagtaaaca
     1081 aaaatctgct aagtgggtgt tagaaggtga cattaaagga tgttttgata atatcagtca
     1141 cgagtggata atgaaacaca ttccaatgaa caagacaata cttggaaaat ggttgaaaag
     1201 tggttatatt gaaaatcgaa agttatttcc aacagagttt ggaagtcctc aaggttcacc
     1261 aatctcacca atcatttcaa atatggtact agatggtatt gagagaaaac tttcaacaac
     1321 atttagaaag aaaaaggtta atggtaaggt ctatgcacca aaaattaatt ttgttaggta
     1381 tgcggatgac tttattgtta ctggtgtcag taaggaacta ttggaaaatg aggttaaacc
     1441 agccattatt gagttcttga aagaacgtgg tttggagtta tcggaagaaa aaacgttgat
     1501 aactcatatc acggatggct ttgattttct gggcgtaaac attagaatgt acgacggaaa
     1561 actactaacc aaaccttcca aaaagaatta cgaaagtatt gtttctaaaa tcagggatat
     1621 tatcaaaaac aacccgtcga tgaaacagga gttgttaatc cgtaagttaa accctattat
     1681 tataggttgg gttaactacc aaaagcacaa tgtttcaaca gaagcatttc aaagacttga
     1741 ttttgatatt tatcagtgtt tatggcagtg gtgtatccga agacatccta aaaaggggag
     1801 aaagtgggtt gctaataaat attttcatac ttttggtagt cgtagttgga tttttagtgt
     1861 tccaactgcc gatacaatgg aaaatggcga gccgttctat cttcgcttga aatatgcttc
     1921 tgatactgat attcgccgac atattaaaat taaggcggaa gccaatccgt ttgatgaaca
     1981 atggcaactg tattttgagg aacgacaaga aaagcaaatg cgacaggaac taaaaggaag
     2041 acgtgtgatt aacggactat attataaaca aaaagggatt tgtccagttt gcgaattgaa
     2101 aatcaccaag gaaacagatt tccgagttca tcaaaccgtg aaagaccata aaccaataaa
     2161 aacattggta caccctactt gtcataaaaa tataaaagaa aacacgctgg ttctctaacg
     2221 aggacttata gaagcttgag ccgtgtgagg ggaaactctc atgcacggtt cttagagggg
     2281 aaagaggttg t
//
LOCUS       DATUCG010000201         2259 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.204, whole genome shotgun sequence.
ACCESSION   DATUCG010000201 DATUCG010000000
VERSION     DATUCG010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.204"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            <1..>2259
                     /locus_tag="VCA20_002344"
     CDS             <1..>2259
                     /locus_tag="VCA20_002344"
                     /inference="COORDINATES: protein
                     motif:HMM:NF018196.1,HMM:NF037691.1,HMM:NF037692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEP1836062.1"
                     /translation="AVRTNVDALVLELRKYLGTDIIEIALNTSLTSGLKVGEGEGILV
                     EKGTTATPSMDDANGAGIKPHTLRTGVTATTEDGWYTFESYDLYSYNANMDGTIFNGT
                     ALKAYVRFSLDSDPSTQTVLAELVSTTDGQVLEKYKVDPDSSVVFTYPKKINPNNENL
                     KVSYETQTAVDGSTFGNVRIIGDTRGSIYNNTIIPTYQVNTTTYKVEDGRILALYNLQ
                     TIAGQTVTSSGQREFAGYEFVKKIVEPAQGGYPVGMKYVAGVVNTATDKSNTIQYKVI
                     RTIVSNTEYVHEIYVLDPTYTGTVDWDGLDTRGFFKLLETSHMSYTPDKVSYDYNTNP
                     TITANYKVDPDTGYMVFAESPLNDKGSKYRVVAQWSGTGEMGKYGKIFIGSQVWTKAD
                     TGANTPFAWQSVSGSYVNLGYSPYGIQQTLRGSSTPAALTTTYVYRALNQEAIIRYQV
                     EGATDYLEDSGILTGGSGTDINYSTEETINEYKKLGYELVSDNFTTSGGQDYDYDTAV
                     LQEFLVVIKPRVVDVPKGVVPRKPVDPTDPNSPIWPDTVNNLETSQEVTRTVEYKYED
                     GTPVRVDAAGNVLPKGSTEGTPFVKTETVSFTRPAQVNLVTGKVTYGDWVADTTDTLQ
                     GNVLPEVPGYSASRTTLEGADAPMTETVKDKTVTATDADINEVVYFKENPKYGDVVVN
                     YVNTDGKVIATPVVDTNDALVGTDYSTADRVPEKIVEDETGDVYYYKEIKAEDAAKET
                     GKVVEGTTEVTYV"
BASE COUNT          729 a          416 c          492 g          622 t
ORIGIN      
        1 tgcagttcgt acaaacgtag acgctttagt cttagaattg cgtaagtact taggaacaga
       61 tattatcgaa atcgctttga atacctcatt aacatcaggt cttaaagttg gtgaaggcga
      121 aggaattctt gttgaaaagg gtacaacggc tacaccgtca atggatgatg cgaatggagc
      181 aggtatcaag ccacacactc ttcgaactgg cgtgacagct acaactgaag atggatggta
      241 tacttttgaa tcgtacgact tgtattcata taatgcaaat atggatggta ctatatttaa
      301 cgggacagct ttgaaagcct acgttcgttt ctctttagat tcagatccaa gcacacagac
      361 tgttttggca gaattagtca gcacgacaga cgggcaagta cttgagaaat ataaagttga
      421 tcctgattca agtgtagtct ttacgtatcc aaaaaaaatt aaccccaata atgaaaacct
      481 caaggttagt tatgaaaccc aaactgccgt tgatggaagt actttcggaa acgtcagaat
      541 aattggtgat acaagaggtt caatatacaa taatacaatc attcctacct atcaagttaa
      601 cacgacaacc tacaaagtag aagatgggag aattttagca ctctacaacc tacaaacaat
      661 cgctggacag acagttactt cttctggaca acgtgaattt gctggttatg agtttgtcaa
      721 aaagattgtg gaacctgctc aaggtggcta tccagtcggt atgaaatatg tggctggtgt
      781 agttaatact gcaactgaca aatccaatac gattcaatac aaggtaattc gtactattgt
      841 atcaaataca gaatatgttc atgagattta tgttttggat ccaacttata cgggaacagt
      901 agactgggat ggccttgata ctcgcggatt cttcaagttg ctagagactt ctcatatgag
      961 ttacactcca gataaagtaa gctatgatta taataccaat ccaacaatta ctgcaaatta
     1021 caaagttgat cctgacacag gctatatggt gtttgcggaa tctcctctaa atgataaagg
     1081 ttctaaatat cgtgttgtag cccaatggtc tggtactggg gaaatgggta aatatggtaa
     1141 aatttttatc ggttctcaag tatggaccaa agcggataca ggtgctaata ctccttttgc
     1201 atggcagtct gttagtggtt catatgttaa cttgggctac tcaccttacg gtattcaaca
     1261 gactttgcgc gggtcttcca cacctgcagc tttgacaact acttatgtgt atcgtgcact
     1321 taatcaagag gctatcatcc gctaccaagt tgaaggtgct acagactatt tagaagattc
     1381 aggtatattg acgggtggtt caggaacaga tattaactac tcaactgaag aaacgattaa
     1441 tgagtacaaa aagctaggtt atgaacttgt ttcggataac tttactacct caggcggtca
     1501 agactatgat tatgatacgg cggttctaca agaattcctt gttgtcatca agcctcgtgt
     1561 cgttgacgtt cctaaaggtg ttgtaccgag aaaaccagtg gatccaacag atccaaactc
     1621 accaatctgg ccagatacag taaacaatct ggaaacaagc caagaagtaa cgcgtactgt
     1681 tgagtacaag tatgaagatg gaacgccagt ccgtgtagat gccgcaggca atgtcttgcc
     1741 aaaaggttct actgaaggga ctccatttgt taagactgaa acagtaagct ttactcgccc
     1801 agctcaagtc aaccttgtaa ccggtaaagt gacttatgga gattgggtgg cagatacgac
     1861 agatactctt cagggtaatg tccttccaga agttccaggc tacagtgcaa gtcgtacgac
     1921 tcttgaaggt gctgatgcac caatgacaga aacagtaaaa gataagactg ttactgcaac
     1981 tgatgcagac attaatgagg tggtttactt caaagaaaat cctaagtatg gtgacgtagt
     2041 tgtaaattat gttaatacag acggaaaagt gattgcaact ccagtggtgg atactaatga
     2101 tgctcttgta ggaacagatt actctactgc tgatcgtgta ccagagaaga ttgttgaaga
     2161 tgaaactggt gatgtatact actacaaaga aatcaaagct gaagatgctg ctaaagaaac
     2221 aggtaaagta gtagaaggta caacagaagt tacttatgt
//
LOCUS       DATUCG010000202         2229 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.205, whole genome shotgun sequence.
ACCESSION   DATUCG010000202 DATUCG010000000
VERSION     DATUCG010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.205"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(74..1990)
                     /locus_tag="VCA20_002345"
     CDS             complement(74..1990)
                     /locus_tag="VCA20_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013793764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HEP1836063.1"
                     /translation="MSQYYQLLKEKFPTKSSLITEMINLDAICHLPKGTEHFLSDLHG
                     EYQAFDYLLRNGSGSIKKKLQECFPEKKEKDIEQLCQYIYYPAEKIQAQQEVLDRKDL
                     ETELCQYLLDLLQFVKHIGSKYTRSKVRKMLPADYAYIMEELLTEEQPDKNKQYYYHA
                     IISKVIELEQLDNLFIAFANLIQRLSIDHLHVVGDIFDRGQYPDFIIDRLKGFQNIDI
                     QWGNHDITWIGAMSGSYICMINVIRIAARYNSLALIEDRYGINLRRLIDYSQRYFKEE
                     EVFNPILDGDVISETERLTLNKLQQATALLQFKLEHQLIKRRPEFGMQTSQLFPKIDP
                     EKKSITIGEKTYPLQSFPFHKMVWDQPDVLTSEEEQILKGLMYRFQNSEKLESQIAFL
                     MAKGSMYHISNNHLLFHGCMPMHSNGHFKSMRFGKKIYSGKALLDFFQEQVRESYKNR
                     EQHFDLATDVFWYLWCGEVSSLFGKDRMTTFERYYIADKASHHEEKNPYYRLRENQEI
                     CLHILAEFGLDGSAHIVNGHTPVKEKGGELPIKADGRIIIIDGGLAKGYQKTTGIAGY
                     TLISNSYGLELVAHKPFSSVKDVLEGKCDIIFIKRLVELVENRTLVKDTDNGKKLLQA
                     IADLDHLYHHFDQY"
BASE COUNT          705 a          424 c          403 g          697 t
ORIGIN      
        1 tatgtcaggc tgagccgcaa aaataaaaaa aacaaaagag gtgaaaattt ggcttcacct
       61 ctcataattc tatttaatat tgatcaaaat gatggtaaag atgatcaaga tcagctattg
      121 cttgtagcaa ttttttacca ttatcagtat ctttaaccaa ggtccgattt tcaactaatt
      181 cgactaaacg cttgataaag ataatatcac atttaccttc taaaacatcc ttaacagatg
      241 agaatggttt atgagcaact aattccaagc cataagaatt ggaaattaag gtgtagccag
      301 caataccagt cgttttttga tagcctttag ccaatccacc atcaatgatg ataatacgac
      361 catctgcttt aataggtaat tcgccacctt tttccttaac aggtgtatgg ccattgacaa
      421 tatgagcact tccatccaaa ccaaactctg ctaaaatgtg aagacagatt tcttgatttt
      481 cccgtaaacg ataataagga ttcttttcct catggtggct tgccttgtca gcaatataat
      541 aacgttcaaa agtggtcatc cggtccttac caaaaagtga tgaaacctca ccacaccaga
      601 gataccagaa gacatcggtt gctaaatcaa agtgttgctc ccgatttttg tatgactccc
      661 taacttgctc ttggaagaaa tctaagagag ccttgcctga atagattttc ttgccaaaac
      721 gcatggattt aaaatgacca tttgaatgca ttggcataca accatggaaa agcaagtgat
      781 tattggaaat atggtacatt gagccttttg ccatcagaaa agcaatttga ctctctaatt
      841 tttctgagtt ttggaaacgg tacatgagcc cttttaagat ttgctcctct tcagacgtta
      901 aaacatctgg ctgatcccaa accattttat gaaatgggaa ggattgtaaa ggataggtct
      961 tttcaccaat agtaatgctt ttcttttctg ggtcgatttt agggaataat tgtgaagttt
     1021 gcatgccaaa ttctggacgc cgcttaatta attggtgctc caatttaaat tgcaagaggg
     1081 ctgtcgcttg ctgtaattta ttaagggtca gacgctctgt ttcagagatg acgtcaccat
     1141 ctaaaatcgg gttgaatacc tcctcttcct taaagtaacg ttggctgtaa tcaataaggc
     1201 gtcggagatt aataccgtag cgatcttcga ttagtgctaa cgaattatat ctagccgcaa
     1261 tccgaatgac attgatcata caaatatagg aaccagacat agctccaatc caagtaatat
     1321 catgatttcc ccattgaata tcaatgtttt ggaaaccttt taagcggtca ataataaaat
     1381 cggggtattg gccgcggtca aaaatatcac caaccacatg aagatggtcg atggataatc
     1441 gttgaatcaa gttggcaaac gcaataaaaa gattgtctaa ctgctctaac tcaatcactt
     1501 ttgagatgat agcatgatag taatactgct tatttttgtc aggctgctct tccgtcagca
     1561 actcctccat gatataggca taatcagctg gcagcatctt acgaacctta gaccgggtgt
     1621 atttgcttcc gatatgctta acaaattgga gtaaatctaa cagatattgg caaagttcag
     1681 tttccaggtc tttacggtct aaaacttctt gctgagcttg aattttttca gcagggtagt
     1741 atatatattg gcaaagttgt tcaatatctt tttctttttt ctctggaaaa cattcttgta
     1801 gtttcttttt aatggaacct gagccattac gtaacaaata atcaaatgct tgatattctc
     1861 cgtgtaagtc acttaggaaa tgctcggtac cttttggtaa atggcaaatg gcatccagat
     1921 tgatcatttc ggtaataagt gacgacttgg ttggaaattt ttctttcaac aactgataat
     1981 attgagacat gattggcagt gggtcctttc tattttcaaa aaacaataac cttttaaaga
     2041 ggcctctatt accattttaa aacttattta cctaaatggc tagtaaaaac tgcaagcgca
     2101 tacaaaaaag agagcttcac ttctctccca atgtcattaa gttaagcatt taagcagcta
     2161 aagaaactat tcccgtctga gcaattgatg acaaaacggg aatagttttt gtatttaggg
     2221 cgcacaaaa
//
LOCUS       DATUCG010000203         2225 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.206, whole genome shotgun sequence.
ACCESSION   DATUCG010000203 DATUCG010000000
VERSION     DATUCG010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.206"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(98..1720)
                     /gene="groL"
                     /locus_tag="VCA20_002346"
     CDS             complement(98..1720)
                     /gene="groL"
                     /locus_tag="VCA20_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940029.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEP1836064.1"
                     /translation="MAKEIKFAADARESMVRGVDILADTVKVTLGPKGRNVVLEKAYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPIGIRRGIEAAVATAVEALKAQASPVSNKAEIAQVAAVSSRSEKVGEYI
                     SEAMERVGTDGVITIEESRGMETELDVVEGMQFDRGYLSQYMVTDNEKMVAELENPFI
                     LITDKKISHIQDILPLLESILQTNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLDLKDATIEALGQAAKVVVDKDGTTVVEGA
                     GNPEAIANRVAVIKSQIEVTTSEFDREKLQERLAKLSGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALVNVIDSVAKLELKGDDETGRNIVLRALEEPVRQ
                     IAYNAGYEGSVIIDKLKNSELGTGFNAATGEWVNMMDAGIIDPVKVTRSALQNAASVA
                     SLILTTEAVVANKPEPATPAMPQGMDGMGMGY"
     gene            complement(1731..2012)
                     /gene="groES"
                     /locus_tag="VCA20_002347"
     CDS             complement(1731..2012)
                     /gene="groES"
                     /locus_tag="VCA20_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEP1836065.1"
                     /translation="MLKPLGDRIAVKIEEKEQTVGGFVLAGASQEKTKEASVLAIGQG
                     IRTLNGDLVAPAVAVGDTVLIDAHAGLEVKDGDQNVHIIRETDILAIVE"
BASE COUNT          584 a          558 c          407 g          676 t
ORIGIN      
        1 tattattgta ttaaaagtat ttagggccgc acttatcatc ataggctcaa aacaacacaa
       61 aaaacacagc caaacagctg tgtcatttct gtattttcta atagcccata cccattccgt
      121 ccataccttg tggcatagct ggagtagctg gttctggttt gttggcaaca actgcttctg
      181 tcgtcaaaat caagctggct acagaagccg cattttgaag agcagaacga gtaactttaa
      241 cagggtcaat gatacctgcg tccatcatgt tgacccattc gcctgtcgct gcgttaaagc
      301 cagtgcccag ttcagaattc ttcaacttat cgatgataac tgaaccttca tagcctgcgt
      361 tataggcaat ctgacgaact ggttcttcca aggcacgaag gacaatattg cgtcctgtct
      421 catcatcgcc tttcaactcc aatttcgcta cgctatcgat aacattgaca agggctgtac
      481 caccaccggc aacgattcct tcttcaactg cggcacgtgt tgcattgagg gcatcttcga
      541 tacggagttt catttctttc aactcagtct cagtagcagc accaactttg atgacagcga
      601 caccacctga caatttagcc aagcgctctt gtaatttttc acggtcaaat tctgaagtgg
      661 ttacctcaat ttgtgacttg ataacagcga cacggttggc aattgcttct ggatttccag
      721 caccttcaac aacggttgta ccatctttat cgaccacaac tttagctgct tgaccaagtg
      781 cttcaatggt cgcatctttc aagtccaaac caaggtcttc tgtaatcact gtaccacctg
      841 tcaagatagc aatgtcttcc aacatagctt tacgacggtc accaaatcct ggcgccttaa
      901 cagcaacaac gttgaatgta ccacgaatct tgttgagaac aagggtagga agagcttcgc
      961 catccacatc gtcagcaata atcaacaatg gacgattggt ttgaagaatg ctttccaaaa
     1021 gtggcaaaat atcttggata tgagaaatct tcttatccgt aatcaagata aatggatttt
     1081 caagttctgc caccatcttt tcattatctg ttaccatgta ttgtgataga taaccacgat
     1141 cgaattgcat accttctacc acatccaact cagtttccat accgcgagat tcttcaatgg
     1201 tgattacgcc gtctgttccc acgcgctcca tagcctcaga aatgtactca ccaacttttt
     1261 cagaacggga agataccgct gctacctgag caatttcagc tttattggat acaggacttg
     1321 cttgtgcttt caatgcttca actgccgtcg caaccgcagc ttcgatacca cgacggatac
     1381 caattggatt ggcacctgcc gttacgtttt tcaagccttc acgtacgata gcctgagtca
     1441 acacagtggc cgttgttgta ccgtcaccag cgatgtcatt ggtttttgaa gccacttcag
     1501 ataccagttt ggcacccata ttttcaaaat ggtcttccaa ttcgatttct ttggcaatgg
     1561 tcacaccgtc gtttgttata agtggtgaac cgtaagcttt ttccaagaca acattacgac
     1621 cttttggacc caaggttact ttgactgtgt cagccaaaat atcgacacca cggaccatgc
     1681 tttcacgcgc atctgctgca aatttaattt cttttgccat ttttctacct ctattctaca
     1741 attgctaaaa tatctgtttc acggatgatg tgaacgttct gatcaccgtc tttgacttcc
     1801 aatcctgcgt gagcatcaat caaaacagta tctccaactg ccaccgctgg ggcaaccaag
     1861 tctccgttca aggtccgaat cccttgtccg atcgctagga cacttgcttc ttttgtcttt
     1921 tcttggcttg cgcctgctag gacaaagcca ccaactgttt gttctttttc ttcaattttt
     1981 acggcgatac gatcgcccaa tggtttcaac atggtattac ctcctggtct ttttagcact
     2041 ctttgtattc gagtgctaat tcacatttcc tattgtatca cttggtcaaa aatagtcaag
     2101 aacattctat ccctttttca tgaaaatcca tcttgagacc gagacagaac ttgcctatcc
     2161 attaccgcta aattatgcta tactattttt aaaaagttag ggggtattca tgaaattttt
     2221 tagag
//
LOCUS       DATUCG010000204         2223 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.207, whole genome shotgun sequence.
ACCESSION   DATUCG010000204 DATUCG010000000
VERSION     DATUCG010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.207"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(101..855)
                     /locus_tag="VCA20_002348"
                     /pseudo
     CDS             complement(101..855)
                     /locus_tag="VCA20_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768029.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
     gene            922..1348
                     /locus_tag="VCA20_002349"
                     /pseudo
     CDS             922..1348
                     /locus_tag="VCA20_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611996.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1700..>2223)
                     /locus_tag="VCA20_002350"
     CDS             complement(1700..>2223)
                     /locus_tag="VCA20_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEP1836066.1"
                     /translation="GKSYDTTDNKPTEIVTEDGSRYVLIPSKTIGTENGTVEGGKTTE
                     VTYVYRKVTPAKKVVTNHVDEDGNPIAPQEEGTTPNKSIPGYEFTGKTVTDPDGNTTH
                     IYRKINNSVKPTPPTTDTPSTPKPNAPMKASGVAQLPNTGESSSSVVTALGAGMLLAS
                     LGLVGKRRKDSEE"
BASE COUNT          675 a          450 c          422 g          676 t
ORIGIN      
        1 atgggctcat atcaaaaaac acctcagaag agtattgccg aattgcgata cgtttcttga
       61 ggtactttag tcctgctctt gtttcagttg actatacttg tggcaaggac aatttgaagt
      121 ttgctttgat ggatttttct gccccttcaa taatccaaga catgatggct ggtgctgctt
      181 cgtcgtaaag gtagtccgca aagtttttga tatctgactg accggtgatt ttggcattaa
      241 aaggaatgac aatcagtcgt ctccaaattc catcatcatt cgcacctact ttaggcagat
      301 gattggtgta aagaaccagc gtatgtgatg gtacaaaatg gaaaggtgcc ttgtactttt
      361 tctcagctaa gatttcatct gtcgaacaga gctgtttaac catagctgta ttcatcgcat
      421 tccctcgctc atctctgaag caatgatgag acattttcct ttcaactcag ccatctcagg
      481 actgacattt cgttgaacac tcatagtcag agcatcagcc gataattttc ctgaatagtt
      541 accaagaact cttgcgatag tgttccaaaa ggtggacttg ccatttgcac cactgccata
      601 agcgattatt aaatgttctt gatagacctt tccaatagca gccataccca ccaccatccg
      661 aacgtagtcg attagttctt gattaccaca aaagaaagtt gctagagtct ctagccacaa
      721 gccttttcct ttatcacttg gtgaggcatt ggtcattttg gttagataat cagcagaatc
      781 atgttcccaa acaccagaca agccttgtga caaatcaata gtcgcttctg gagtattcag
      841 taagagttca tttgaacagg ttacactaaa ctagatagaa tttataaagt gttctacact
      901 aaagaaaata ggagaaaaac tatatctaga aaaattcgcc gttacttcac agatgacttc
      961 aaacaacaaa tcgttgactt gcacaatgca ggtagaaaac gcagtgaact gatcaaagaa
     1021 tatgatttaa cgccctcaac cttcgataag tgggttaaac aagcaagaac aactggttcc
     1081 tttaaaactg tcgataatct gacagatgaa caacgtgagt tgatagaact cagaaaacga
     1141 aataaagagc tcgaaatgca attaaacatt ctaaagcaag cggcagtgat tatggcgcga
     1201 aaagggaata atcactgcta acaaggataa atatagcatt tcagacatgt gtcgttggtt
     1261 gaagattcct cgctctagct attactagaa agctgttaag ccagtacctg aagcagaact
     1321 tggagaaaaa atcaaatcta tcttcctcgg attcgtcgca tcatgaagag attgatactc
     1381 atttaaaatc aaaaatagcc acctaccact caaagtggat atggagcgtt ttgattttaa
     1441 atgagtatta tagtgttaat aacccactcc taaaataact ttagagttct caaattgaca
     1501 aacttatata tgtcctgctt ctaattcaat cgactataca acatgtccgt cgaactcaaa
     1561 aaagagagaa tcgttccgat tctctcaaga tattaataat aagtcaccta ctatagttga
     1621 agaagagccg tttttaccag acgctagaca ttaatctttc gcctactaaa cagatttgct
     1681 aaaactagtt ttagttggac tattcttcgc tatctttacg acgtttccct actaatccga
     1741 gacttgctag taacatacct gcaccaagtg ctgttactac agaagaacta gattcaccag
     1801 tatttggcaa ttgagccaca cctgatgcct tcataggagc attaggtttc ggtgtagaag
     1861 gtgtatcagt tgtaggcgga gttggcttaa cagaattatt aattttacga taaatatgcg
     1921 ttgtgttgcc atctggatct gttacagtct tacctgtgaa ctcgtatcct gggattgact
     1981 tgttcggtgt tgtaccttct tcttgcggtg caattgggtt accgtcttcg tcaacgtggt
     2041 ttgtgacaac tttcttagct ggagtcactt tacggtaaac gtaggttact tcagttgtct
     2101 taccaccttc aactgtacca ttttctgtac caattgtctt agatgggatc aatacataac
     2161 gtgaaccgtc ttctgtaaca atttctgttg gtttgttatc agttgtgtca taagacttac
     2221 cag
//
LOCUS       DATUCG010000205         2218 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.208, whole genome shotgun sequence.
ACCESSION   DATUCG010000205 DATUCG010000000
VERSION     DATUCG010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.208"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            20..247
                     /locus_tag="VCA20_002351"
     CDS             20..247
                     /locus_tag="VCA20_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1836067.1"
                     /translation="MSAIERLAEKVARQEEKVSRETEKLEHYRDQLQTAMYSTFIKRQ
                     QSSRLSFHEALEQAFGKETTLQPNYRNEDIE"
     gene            244..633
                     /locus_tag="VCA20_002352"
     CDS             244..633
                     /locus_tag="VCA20_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HEP1836068.1"
                     /translation="MSKTWNFDQPLDDVKSTSSHEERAKIAALFHKQDEKPIEEVDYV
                     AAFEQQQKESKSKDVQMLASTVKQNQPKKVNITSDYKQHLADTIAQNNKDISACQKQI
                     EELHQLIDEKKIQNKKLQTISVAIDDL"
     gene            683..979
                     /locus_tag="VCA20_002353"
     CDS             683..979
                     /locus_tag="VCA20_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1836069.1"
                     /translation="MKFFQKKKQNQDQFKRLIHRLSEMSDTELTKVEQLLDVVFDTNF
                     KPDRNVELSQSVISDEDQSLDRSIQEAKNKLNMEQLEKNIERFRQGRRESNNNK"
     gene            1157..>2218
                     /locus_tag="VCA20_002354"
     CDS             1157..>2218
                     /locus_tag="VCA20_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HEP1836070.1"
                     /translation="MPNNVFELINFKDYLPLDLDITDKTRLVYRPVLAPTQLKCSLEP
                     ALTEEQLYVHMMKENSNWSFEYDGNDDFYKQDCVDFLRDTDFQSFSQYGKLVDRKYIE
                     AKRVYLFDSYFRNFLQEILERIEVFLKKSTADALTIGYNKPSYIFEDDALYYSEQSKY
                     RKENPNRQDIVLKTKYHLSRLILEKQDDATIKNQIDKYGVVLPWTVFRLMTFGNIASF
                     LVALQPEHRNKVAAYISLPLDKEDNIPAKILLSWCNALRYLRNICSHNGRLYGRLHHT
                     LPALHHSDKELLMTGSENDDKTLFIYFITMRHLILSMSLETHSFWNKKLQKLLEESHQ
                     EQVDLRHYGFPKNWMDLLAI"
BASE COUNT          802 a          365 c          416 g          635 t
ORIGIN      
        1 gtgacaagga ggacacccta tgagcgcgat tgaacgtctg gctgaaaaag tagctaggca
       61 agaagaaaag gtctcacgtg agacggagaa attggaacac tatcgtgacc aactacaaac
      121 agctatgtac agtaccttta tcaaacgaca acaatctagt cggttgtcat ttcatgaagc
      181 actagagcaa gcctttggta aagaaaccac actacaacca aattacagaa atgaggatat
      241 agaatgagta aaacatggaa ttttgatcag ccactagacg acgtgaaatc aacatcgtcc
      301 catgaagaac gagctaaaat cgcagcactc ttccataaac aggatgaaaa accaattgaa
      361 gaagtagatt atgtggctgc ttttgaacag caacaaaagg agtcaaaatc aaaagatgta
      421 caaatgctcg catcaaccgt taaacaaaat cagccaaaga aggtaaatat cacaagtgac
      481 tataaacaac acttggctga taccattgca caaaacaata aggatatttc cgcctgtcag
      541 aagcaaattg aagaacttca tcaattgatt gatgaaaaga aaatccaaaa taaaaagttg
      601 cagactattt cagtagccat tgatgattta taagtcaggt agtcctatgc gggctacctt
      661 tttacaatgt atggagaaga taatgaaatt ttttcaaaag aaaaaacaaa accaggatca
      721 atttaaacga ctgattcatc gactatctga gatgtcagat actgaactaa ccaaagtaga
      781 acaactccta gatgtggttt ttgatacaaa ttttaagcct gataggaacg ttgaattatc
      841 tcaaagcgtt atatccgatg aggatcaaag tcttgatagg tcaatccagg aagcgaaaaa
      901 taaacttaac atggaacaat tggaaaaaaa cattgagaga tttagacaag gtaggcgaga
      961 gagcaataat aacaaataac agaccaatta ttttggcctg ttttttgaaa tatgtgtcat
     1021 cgttgaatca aacgaagtaa aaatatttct tgcttagtag gaggtgttgt ggtataattt
     1081 agttagatta gaagtcatcg aaggtacagc aatgtataac taggtggcct tttttcatat
     1141 tgcggagaga tattttatgc ctaataatgt atttgaatta ataaatttta aggactattt
     1201 gccattagat ttagatatta ctgataaaac taggttggtt tatagaccag tattagcacc
     1261 aactcaatta aaatgttctc ttgagccggc tttaactgaa gaacagttat atgtccatat
     1321 gatgaaagaa aactctaact ggtctttcga gtacgatggg aatgatgact tttataaaca
     1381 ggattgtgtt gattttttac gagatacaga ttttcaatcc ttttctcaat atggaaagtt
     1441 ggtggacaga aaatatatag aagcaaaaag agtttactta ttcgatagct actttcgaaa
     1501 ttttcttcaa gagatactgg agcgtatcga agtttttttg aagaagagta cagctgatgc
     1561 tctgacgatt ggctacaata aaccttctta tatttttgaa gacgatgctc tctattattc
     1621 agaacaatct aaatatcgaa aagaaaatcc taataggcag gatattgttt tgaaaacaaa
     1681 atatcatttg tcacgattga ttttggaaaa acaagatgat gccacaatca agaaccagat
     1741 tgacaaatat ggtgtcgtgt tgccttggac cgtttttcgt ttaatgacat ttggaaacat
     1801 agcgtccttc cttgttgccc tacaaccaga acatcgaaat aaggttgctg cctatattag
     1861 tcttcccttg gacaaggagg ataacattcc agctaaaatc ttattgtctt ggtgtaacgc
     1921 tcttcgttac cttagaaata tttgctcaca caacggtaga ttatatggtc gcttacacca
     1981 cactttaccg gctcttcatc attctgacaa agaattattg atgacaggtt cagagaatga
     2041 tgacaagaca ctatttatct attttataac aatgagacat ctcatcttat ctatgtccct
     2101 agaaacccat agtttttgga ataaaaaatt acaaaagctt ctggaagaaa gtcatcaaga
     2161 acaagtggac ttgagacatt atggttttcc aaaaaattgg atggacttat tggcaatt
//
LOCUS       DATUCG010000206         2188 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.209, whole genome shotgun sequence.
ACCESSION   DATUCG010000206 DATUCG010000000
VERSION     DATUCG010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.209"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            1..934
                     /locus_tag="VCA20_002355"
                     /pseudo
     CDS             1..934
                     /locus_tag="VCA20_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775141.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="G5 domain-containing protein"
     gene            932..1123
                     /locus_tag="VCA20_002356"
                     /pseudo
     CDS             932..1123
                     /locus_tag="VCA20_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072409.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4368 domain-containing protein"
     gene            1300..1941
                     /locus_tag="VCA20_002357"
     CDS             1300..1941
                     /locus_tag="VCA20_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1836071.1"
                     /translation="MIRELKDDGKSYIKIFLIGILVGCITRLLDYCSLDTLWSWSSIQ
                     TLLGFWMISNTIIVVKSSSNKCAGISSFLYMFGMTLSFYGLQVILGMFIPMFSDRFRF
                     SLFIMFTILSIPCAIAAFILYYWNKNEFYNSVLYSLPVGALFSETVAIAIKFHMTHTF
                     LFQLLMDGIGTIAFGVLFFKKVKNRYVYLFSIIISFLVFYFILYHREVLTWLE"
BASE COUNT          742 a          334 c          451 g          661 t
ORIGIN      
        1 ttggtgaaaa acgacaacgg ttcggttttc ggaaactgtc aattggactt gtcttggcag
       61 ctgtagcaag tttgttttgg tgtcatcggt cgcagcagca ccttcagcta gtgctcagtc
      121 tatcgattat tcctatgtga cagagcaaga attgacagat gcagaaaagg agttgattgt
      181 tcgtgacttg cctggcttag ctcaggaaac ggatgcaaat tattacctga tctatcgtcc
      241 agctactggc acagcatcta ctccatcgac aagcacgtct caagttttgt caaatactgg
      301 ctctgtagaa accggactat tcgtggcggg tggagtgtct ttcctattat tggctgttag
      361 aattggtaaa aaaggtaaga aggagttggc gggtgtcatc cttttgacag ctacgggagc
      421 aagcttcttc ggtccaacta gctccgcatt gacgagtcaa attttggctc aatataacca
      481 tgcaatcgaa atatctgccg gacaagcgtt accggcccca gctgagattg atggctacgt
      541 ttatgtagga tatttgaagg atagcaaagc tattgaacaa actgcttcag aaggaatccg
      601 tacagaaacc attgtcaaca agacagaggc cattccattt gaaattcaaa ccgtagaaaa
      661 tccccaatta tctgcgggga cagagcgagt ggtacaggaa ggacaggatg gggagcgtac
      721 tgtaacgatt aaacaagtcc attcaaaagg tcaaattatt tcggaagaaa agatttcctc
      781 gacggttaca aaaactgctg ttccaaagat tgttgaagtt ggtacagaaa aagcaaccga
      841 tgatattgtt acagaagtac cagatacaga accaagttat gaaaactcga cgaattcttt
      901 aacagaagaa acagttactc ataccgaagt ctttgataga ttatttaata cctatgatac
      961 agagcaacga gacttagaaa agcaaataca gtattttgaa aatgaaatag aaagctatca
     1021 tcagagaaaa gttgacaccg ataaattcct aaaaatgata gagaaatata cagatattga
     1081 agaactgaca gtaccaatga taaatgagta tatagaaaaa gtagtaatcc atgaagccac
     1141 agggggaaga caaggcaaag accgaaaaca acaagtcgat gtgtatttta actttatagg
     1201 caactgtcaa gtaccacaaa aagccaatat agaaaaaatg gcttaaaaat agtataatat
     1261 agttggataa atttgaattt atgatggaga tagaatgtaa tgataaggga gttaaaagat
     1321 gatggaaaat catatataaa aatattttta attggaatat tggtgggatg tattactcga
     1381 ttgttagatt actgttcatt agatacttta tggagttggt cttctattca aacgctatta
     1441 ggattttgga tgatttcaaa tactataata gtggtaaaga gttcatcgaa taagtgtgca
     1501 ggaattagct cattcttata tatgtttgga atgacattga gtttttatgg attgcaggtt
     1561 atccttggta tgtttattcc aatgttttcg gatagatttc gtttttcttt atttataatg
     1621 tttacgattt tatccatacc atgtgctatc gcagcgttta tcctttatta ttggaataaa
     1681 aatgaatttt ataactctgt tttatattca ttgccggtag gagcattatt ttcagagacg
     1741 gtagctatag caataaaatt tcacatgaca catacttttt tgtttcagtt actaatggat
     1801 ggaattggaa ctattgcttt tggagtattg tttttcaaaa aagtgaaaaa tcgctatgtg
     1861 tacttgtttt ctatcatcat aagtttctta gtattttact ttattctata tcacagggaa
     1921 gttcttactt ggttggagtg aaattattct atatagatta taaaggaacg agacattatt
     1981 ttaactaaat cccagtttgt cgagctgaat aaaactgaat attaaagata tagggcgata
     2041 gaaagtaaaa catctatcgt cttttcttat gctcaaaatt tgaaagagag gaaaacagaa
     2101 aatgaaaaat atatagtcaa ctgaaacaag agcaggacga aagtatctca agaaacgtat
     2161 cgcaattcgg caatactctt ctgaggtg
//
LOCUS       DATUCG010000207         2177 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.210, whole genome shotgun sequence.
ACCESSION   DATUCG010000207 DATUCG010000000
VERSION     DATUCG010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.210"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(841..2040)
                     /locus_tag="VCA20_002358"
     CDS             complement(841..2040)
                     /locus_tag="VCA20_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEP1836072.1"
                     /translation="MAKLTVKDVELKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYILEQGGRAILFSHLGRVKEEADKEGKSLAPVAADLAAKLGQDVAFIAGATRGAELE
                     AAINALEDGQVLLVENTRFEDVDGKKESKNDEELGKYWASLGDGIFVNDAFGTAHRAH
                     ASNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLE
                     KADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKALLEKANGKLILPVDSKEANA
                     FAGYTEVRDTEGEAVSEGFLGLDIGPKSIAEFDEALTGAKTVVWNGPMGVFENPDFQA
                     GTIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPG
                     LAALTEK"
BASE COUNT          606 a          521 c          387 g          663 t
ORIGIN      
        1 ctaaatacaa aaactattcc cgttttgtca tcaattgctc agacgggaat agtttcttta
       61 gctgcttagt tgcttaactt aatgacattg aatacctgcc cagcctttct tttctcctaa
      121 gctcgactgg aaaagcctcc tgttcacgcc agcttttcga aaaaccaata atctgccact
      181 ttaaatcagc tacaagaagg aacccagaag taagacaaac aaatagacgc tgcaaaaaaa
      241 caacgtcttt ttttgcattt atatttttgc taagtaactg aatggagttg tgaccaagat
      301 tcagccttgc ggggttcaaa tagtccagtg gactatttga agttgtcacc caataaaaac
      361 aaagtgacaa gctacggaat catttttgac agaagcttcg cttcttctat ttccagcctc
      421 aaaatatccc ccagatattt tgaactgacc taccacctat ttttcagtca aggcagcacc
      481 tagccagtct ttctaggtca cacttcccag aaagactagg catggcacta ggcactccgt
      541 gccgtcgcaa gtcctggaag tacatcttgt ttcgcttagc ttcgtagaaa ctctacgcga
      601 tgtacaaaat gcaacggaaa tccattgcat tttagccttt acctaggctg attttctaaa
      661 ctcactacgt tcacaaaaaa tctagccttt gtactagaaa agagttggtc tcccaactct
      721 tttcatcgtt catctttcat acgcagtagc tgaatggcag ttcactttgc tacgcttgtg
      781 accaacctat tcagccttgc gggggtgcgt ctacggaatc atagattcct gacttaccgc
      841 ctatttttca gtcaaagctg caagtcctgg aagcacttta ccttcgagga gttccattga
      901 tgcaccacca ccagttgaga tccatgagaa cttgtctgca cggccaaggt tgatagctgc
      961 tgctgctgag tcaccaccac cgatgattga tttcacgcct ggttgtttca cgatagcgtc
     1021 cattacaccg attgtaccag cttggaagtc tgggttttca aatacaccca taggtccgtt
     1081 ccaaacaact gttttcgcac cagtcaaagc ttcgtcaaac tcagcaattg acttaggacc
     1141 gatgtcaaga ccaaggaagc cttctgaaac tgcttcgcct tctgtatcgc gaacttcagt
     1201 gtatccagca aatgcgttag cttcttttga gtcaactggc aagatcaatt taccgttagc
     1261 tttttcaaga agtgcttttg ccacatcaag cttgtcttct tctacaagtg agttaccgat
     1321 ttcgatacct tgagctttgt agaatgtgta cgtcatacca ccaccgataa gaactttgtc
     1381 agctttttca agaaggttct cgataacacc gatcttatca gatacttttg aaccaccaag
     1441 gattgccacg aatgggcgct ctggagtttc aacagcttct tggatgtaag caatttcgtt
     1501 ttccaaaagg aaaccagcta ctgctttttc tacatttgct gagataccaa cgtttgatgc
     1561 gtgtgcacgg tgtgcagtac caaatgcatc gttaacgaag ataccatcac caagtgaagc
     1621 ccagtattta ccaagttctt cgtcgttttt agattctttc ttaccatcaa catcttcgaa
     1681 acgagtgttt tcaacaagga gaacttgtcc atcttccaaa gcattgatag ctgcttccaa
     1741 ttcagcacca cgagtagcac ctgcgatgaa agcaacgtct tgacccaatt tagccgccaa
     1801 gtcagctgct acaggagcca atgatttacc ttctttgtca gcttcttctt tcacacgccc
     1861 aaggtgagag aaaagaattg cacgtccacc ttgctcaaga atatacttga tagttggaag
     1921 agctgctgta atacggttat cgttagtgat aacgccatct ttcaaaggca cgttgaagtc
     1981 cacacgaaca agaacttttt tgcctttcaa ttctacatct ttaacagtca attttgccat
     2041 tagataagaa ctccttaaat tatatttact ttaccattgt atcatatttt ctgtattttt
     2101 tcttgcataa agatattttt ttcacaaatg ttatgaaatc taaaactatc acctcttaat
     2161 cagatttttc aaattct
//
LOCUS       DATUCG010000208         2140 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.211, whole genome shotgun sequence.
ACCESSION   DATUCG010000208 DATUCG010000000
VERSION     DATUCG010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.211"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            <1..1656
                     /locus_tag="VCA20_002359"
     CDS             <1..1656
                     /locus_tag="VCA20_002359"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012948.1,HMM:NF017548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cna B-type domain-containing protein"
                     /protein_id="HEP1836073.1"
                     /translation="EPQEPEVTKVPVKKVWNVKEGKTVPAQVVIRLMPVNQTLALNAE
                     NNWQGEFTNLPLKGDDGQVINYLISEDEVTGFTTEITGNKETGFVVTNTPEEVTPEIE
                     KIEIPVTKVWSPNTPQDKMPQEITVVLDQTQERLVLNAENGWKGKFENLPKTTEDAKT
                     IDYTISEVTVEGFTSKITGNKETGFIVTNTYNEPEEPQTDKIQIPVQKIWNVKEGEIL
                     PESVEITLQPTGDKLVLSAENQWKGEFTDLPKVNEEGEEITYTITETAVEGYQTDITG
                     DQLNGFTVTNTKEEPEKPEVTEVSVKKVWNAKEGTKIPESITVILEQTGESVVLNAEN
                     EWKASFKDLKAKDEQGNIITYTVKEVEVEGFTSTVSGNMKEGYVIVNTEKPVTPDEPQ
                     KPETRDITVTKKWIGESLDKVVVYLMADGKKIDEVILNKENGWSHTFKGLDKTKDGKE
                     ISYTVEEQEVKGYTVKVEGNIEKGFIITNTKEDPKIPDEPKPNVPNDPGHEEPKNPAP
                     KTPKDRGSVLPKTGDASDIGLYGLSGVLSLVGLILIRRKRKEV"
     gene            1876..2061
                     /locus_tag="VCA20_002360"
     CDS             1876..2061
                     /locus_tag="VCA20_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1836074.1"
                     /translation="MSQPIVPLTIPQSHRFEKKSRNDILMKIHLGKTEVTFFQSVNPE
                     VMETILDKVLYYDHPTQ"
BASE COUNT          840 a          358 c          466 g          476 t
ORIGIN      
        1 gaaccccaag aaccggaagt cacaaaggtt cctgttaaaa aggtatggaa tgtaaaggaa
       61 ggaaaaacag ttcctgcaca agtagtcatc cgtttgatgc ctgttaatca gacacttgcc
      121 ttaaatgcag agaataactg gcaaggtgag ttcacaaatc ttccgctaaa aggagatgac
      181 ggacaagtta ttaattatct gatatctgaa gatgaagtta caggatttac gactgagatc
      241 acaggaaata aagaaaccgg ctttgttgta acaaatacac cggaggaagt aacacctgaa
      301 atcgaaaaga tagagatccc tgttacaaaa gtatggtcac cgaatacacc acaggataag
      361 atgccacagg aaatcacagt ggtacttgat caaacacaag aaagacttgt attgaatgca
      421 gaaaatggtt ggaaaggtaa gtttgaaaat cttccaaaga caacagaaga cgcaaaaaca
      481 attgactata caatcagtga agtaacagta gaaggcttta caagtaagat cacaggaaat
      541 aaagaaaccg gttttatcgt aaccaacact tataatgagc cagaagaacc gcaaacagat
      601 aagattcaaa ttccggtaca aaagatctgg aatgtaaaag aaggagaaat attacctgaa
      661 tctgtggaaa tcaccttaca gccaaccgga gataaactgg tattaagtgc agaaaatcag
      721 tggaaaggag agtttacaga tcttcctaaa gtaaatgaag aaggtgaaga gattacctat
      781 actattacag aaaccgcggt agaaggatat cagacagata ttacaggaga tcagttaaac
      841 ggctttaccg taactaatac aaaagaagaa cctgaaaaac ctgaagtaac agaagtatct
      901 gtcaagaaag tatggaatgc aaaagaaggg acaaagattc ctgaaagcat tacggtaatc
      961 ttggaacaaa caggagagag tgtagtctta aatgctgaaa atgagtggaa agcaagcttt
     1021 aaggatctaa aagccaaaga tgagcaggga aatatcatca cttatacagt gaaagaagta
     1081 gaagtagaag gcttcacaag tacagtaagc ggaaatatga aagaagggta tgtcattgtc
     1141 aatacagaaa aacctgtgac acctgatgaa ccgcagaagc cggaaacaag agatattact
     1201 gtaaccaaga agtggatagg agaaagctta gacaaagtag tagtttacct gatggcagac
     1261 ggcaagaaga tagatgaagt catcctaaac aaggaaaatg gatggagcca tacattcaaa
     1321 ggcttggata agacgaagga cggaaaagaa atcagttaca ctgtggaaga acaagaagtc
     1381 aaaggatata cggtaaaggt agaagggaat attgagaaag gatttattat cacaaataca
     1441 aaagaagatc ctaagatacc tgatgaacca aagccgaacg taccgaatga tccgggacat
     1501 gaagaaccga aaaatcctgc accaaagaca ccgaaggata gaggaagtgt cttaccgaag
     1561 acaggggatg caagtgatat aggactttat ggactaagcg gagtattatc tttagtaggt
     1621 cttatactta ttagaagaaa gagaaaagaa gtatagagag tagttcattt gtctgaatag
     1681 acaagtatac caacaaagag ccaatcaaac tcaagtaaga ggagattggc tcttgttgtg
     1741 gtttgacttt aattcgattt aaccaatagc taatatgctg actatgatat aatgtaaccg
     1801 ccccataaca aggtatctat ccaatcactc cttcctccag tatactgtta taaaaaacac
     1861 actggaggat ttattatgtc acagcccatc gtcccattga ctattcccca atctcaccgc
     1921 tttgaaaaga aaagcagaaa cgatattctg atgaaaattc atctcggaaa aacggaagtc
     1981 acattcttcc aatctgtcaa tcctgaagta atggaaacaa tcttggataa ggtgctgtat
     2041 tatgaccatc cgactcagtg atttaggtca agtcttcttg gtatgcggaa aaacagacat
     2101 gcgtcagggg attgattcac tcgcctatct tgtcaaaagt
//
LOCUS       DATUCG010000209         2097 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.212, whole genome shotgun sequence.
ACCESSION   DATUCG010000209 DATUCG010000000
VERSION     DATUCG010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.212"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            68..406
                     /locus_tag="VCA20_002361"
                     /pseudo
     CDS             68..406
                     /locus_tag="VCA20_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637111.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            698..2095
                     /locus_tag="VCA20_002362"
     CDS             698..2095
                     /locus_tag="VCA20_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEP1836075.1"
                     /translation="MKKVSFWLGINIALLSIMVSLAVWLFAGLQERQVSQFIKEKQQT
                     ILAKGKGKIQEGNIDTTHVVAALPTDDAGHVLGPVESRMISYVQRRFGHKKPAGKIQK
                     LVFVSSIEGRTNFKNVTAREIQAEQYKVDNLQIKKQDKLPSERVLLTQDNELFTLEDL
                     LPNLSSAASIIVDHLREALLAQGMKETDVEAIVKKFETLDLNAISFSYGDSQLTLQLP
                     DGYGINQLVLPISDLYPVVKSDYLVDADKVGYDEYMAAQVVDKKHLRQVALTFDDGPN
                     PNTTPVVLDLLKKYNAKATFFVVGKAVAGNEAILRRMVAEGHVIANHTWNHPNLVTIS
                     GEQVQREIQDTQAAITEATGIVPTMVRPPYGSVNQTVVNQMGLPSIYWSVDSKDWKSR
                     NPQAILKEIKEQTCPGSIILMHDIHQSTVDSLESVLQYLTGEGYNMVTVTDLLASPLN
                     PQLIYYSQELSGPAQ"
BASE COUNT          683 a          357 c          446 g          611 t
ORIGIN      
        1 ctttaaaatt gaaattctct atatacaatt tgattagtag atagctaatt ttgattttaa
       61 atgagtatta gaagcatctc aaaaatttta tgaagaagca tttggattta aggaaacccg
      121 tcgcaaggac tatccagagc acaagtttac cttggtttac ttagcacttg aaggagatga
      181 ctttgagatt gaattgacct ataactacga tcatggtccc tatatcgtag gcgacggttt
      241 ctcgcattta gctctttctt cagatgattt agaaggtgat aatgctaagc ataaggagct
      301 gggctatcca acgacagaca tcaagggctt gccaggaagt ccaggtcgtt attattttgt
      361 gacagatcca gatggctacc gtgtggaagt cattcgtgca gattaattta gaaaagattg
      421 cctaggcagt ctttttcttt tcaaaaaaaa tcagaaaatg gcagaaggga gggaattagg
      481 aagcaaatca attatttata ctcaatgaaa atcaaaagta gcctaggaaa cgaagccgaa
      541 gatggaactg gagttcatta aggaaagttg acaacggata attttgattt tcgaagagta
      601 ttacctaatc tttttatgag tttttataga actgtcttga caagagcctt taaatgattt
      661 agcatagttt gtatatgttt tgaatgggag gttggagatg aaaaaagtta gtttttggtt
      721 gggaatcaac atcgccttgc tcagcataat ggtaagtttg gctgtatggt tatttgcagg
      781 acttcaagaa agacaggtta gtcagtttat caaggaaaaa caacaaacca tcctcgctaa
      841 aggcaagggc aaaattcaag agggaaatat tgatacgacc catgttgtgg ctgccttacc
      901 tacagatgat gctggtcatg tcttaggacc tgtagaaagt cgaatgattt cctatgttca
      961 aagacgcttt ggtcataaaa aaccagcagg aaaaattcaa aaactggtct ttgtttcatc
     1021 aatagaggga agaaccaatt ttaaaaatgt aacagctcgt gaaattcaag cggagcagta
     1081 taaagtagac aacttgcaaa ttaaaaagca ggataaactc ccgtctgagc gtgtgttatt
     1141 gacgcaggac aatgaattgt ttactctgga agatttatta cctaatttat cctctgcagc
     1201 aagtattatt gttgatcatc taagagaagc tttacttgct caaggaatga aagagactga
     1261 tgtagaagcg attgtcaaaa aatttgaaac actagactta aatgctattt cctttagtta
     1321 tggagacagc caattaacct tacaattacc agatggttac ggcatcaacc agttggttct
     1381 accaatttca gatttgtacc cagtagtgaa gagtgattat ctagtagatg ctgataaggt
     1441 tggctatgat gagtatatgg ctgcacaagt tgtggataag aaacatttga gacaggtcgc
     1501 tctaaccttt gatgatggac caaatccgaa cacgacaccg gtagttttag atttactgaa
     1561 aaaatataat gccaaagcaa ctttctttgt tgttggtaag gctgtagctg ggaatgaagc
     1621 tattctccgt agaatggtag cagaagggca cgtcatcgcc aaccatacgt ggaatcatcc
     1681 caatctagtc actatttctg gggagcaagt acagagagaa attcaagata cacaggcagc
     1741 tattactgaa gctacaggta ttgtaccaac aatggttcgt cctccttatg gttctgttaa
     1801 ccagaccgtt gtcaatcaga tgggacttcc atcgatttat tggtcggtcg attctaaaga
     1861 ttggaagagt cgcaatcccc aagccatttt gaaagaaata aaagaacaga cttgtccagg
     1921 aagtattatc ctaatgcatg atattcatca gtcaacagtt gatagcctcg agtctgtatt
     1981 gcaatactta acaggcgagg gctacaatat ggttactgta acagacttac tcgccagtcc
     2041 tctcaatcct caattaatct attattcaca agaattatct ggtccagccc agtaaga
//
LOCUS       DATUCG010000210         2091 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.213, whole genome shotgun sequence.
ACCESSION   DATUCG010000210 DATUCG010000000
VERSION     DATUCG010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.213"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            43..693
                     /locus_tag="VCA20_002363"
     CDS             43..693
                     /locus_tag="VCA20_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEP1836076.1"
                     /translation="MPRILVVEDDIVISQVVCEFLKEHGYQVESVFDGKVALERFQEE
                     QFDLIVLDIMIPSMTGLEVLKEIRKTSQIPILMLTAMGDEYTQLISFNQIISDYVVKP
                     FSPTILVKRIENILRGKGDTDSIEIGTILIQPTSGAVYMEEEEVQLTKKEYEVLLYLA
                     KRRGKIVSRDNLMMGIWGYTELDSRVLDNHIKNIRKKLPSLPLKTVVGRGYQIEDT"
     gene            695..2083
                     /locus_tag="VCA20_002364"
     CDS             695..2083
                     /locus_tag="VCA20_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEP1836077.1"
                     /translation="MGIARKNFIVVAVMISFISVTLLGFLYYAMPIYYNQVKKQELRH
                     DYMTVAKQLDGMPEAKIISEIDDFDRKTPNIIISLFSSDREIIYPDPNDEMAKKHENE
                     YLENGNFDEIGSWGAEITSAEGITYYLLFNYGFHSLSDVSQTLVTFYPFILLLIIVLA
                     VSVAFVYSRLSTRRIARISETTRRMQSLESGISCTVVGTDEITALAQDINSLYSKLLS
                     SIDELRTENERAMAREKEKSDFLRITSHELKTPIASMLGLVEGMIYNVGSFKDHDTYL
                     KKCKEILQEQSELVHSILEATNLDMALKESREQIRLDQLIDSSLTSYQSLAEVKGYQF
                     EVELSPVLIEGNPVYFLKAIKNILDNAFRYSCKEAIIRVVLRQNQLIIENQVERVLSD
                     EELDQVFRPFYRPDYSRDKKDGGTGIGLFIVQKIVEKHGFPYTFYASDTQTMRFMIHL
                     QSQITANSNKLI"
BASE COUNT          703 a          280 c          440 g          668 t
ORIGIN      
        1 ataataatat tttataatag aactttacga ggaggtataa aaatgccaag gatcttggtt
       61 gtcgaggatg acatagttat tagtcaagtt gtttgtgagt ttttaaaaga acatggttat
      121 caggtagaat ctgtttttga tggaaaggtt gctttagaaa ggtttcaaga agaacaattc
      181 gatttaattg ttttagatat catgattccg tctatgacag gtttggaagt actgaaggaa
      241 attcgcaaaa cttctcagat tccaattttg atgctgacag ccatgggtga cgaatataca
      301 cagcttatta gttttaatca gattataagt gattatgttg ttaaaccatt ttcaccaact
      361 atattggtaa aacggattga gaatattttg agaggaaaag gagatacaga tagcattgag
      421 ataggaacaa ttcttattca accaactagc ggggcagttt atatggaaga agaagaagtt
      481 caattgacga aaaaagaata tgaagtttta ctatatttag ctaaacgacg tgggaaaatt
      541 gttagtcgtg ataacttgat gatgggaata tggggatata cggaattgga tagtcgtgtc
      601 ttagataatc atatcaagaa tatacgtaaa aaattgccgt cacttccttt gaagacagta
      661 gttggtcgtg gttatcaaat agaggatact tgatatgggg attgctagaa aaaatttcat
      721 agttgttgct gttatgattt ctttcatctc tgtaacactg ttagggtttt tgtattatgc
      781 catgcctata tattataatc aggttaagaa acaggaattg agacacgatt atatgactgt
      841 ggctaaacaa ttggatggga tgccagaagc taagattata tctgagattg atgatttcga
      901 cagaaaaact ccgaatatta ttattagtct tttttcgagt gatagagaaa ttatttatcc
      961 agaccccaat gatgaaatgg caaaaaagca tgagaacgag tatttagaaa atgggaattt
     1021 tgatgaaatt ggctcttggg gagccgagat aacatctgca gaaggaatta catattatct
     1081 gttatttaat tatggattcc actcgttatc tgatgtcagt caaacattgg taacttttta
     1141 tccattcata ttgctgttga ttattgttct ggcagtttcg gtagcttttg tttacagtag
     1201 gctgtccact cgtagaatag caagaatttc agaaacaaca agacggatgc aatcgttaga
     1261 atccggaatc agttgtactg ttgtaggaac agatgaaata acagcattgg ctcaggatat
     1321 taacagcctg tatagcaagt tattgtcaag tattgatgaa ttaagaacgg aaaatgaacg
     1381 tgctatggca cgtgagaagg agaagtctga tttcctccgt atcacatcac acgaattaaa
     1441 aactccaata gctagtatgt tggggttagt cgaaggcatg atttataatg tggggtcatt
     1501 taaggaccat gatacatatt taaaaaaatg caaggaaatc ttgcaggaac aatctgagct
     1561 agttcattct attttagaag cgacaaatct tgatatggct ttaaaggaaa gcagagaaca
     1621 aatccgatta gatcagttga ttgattcctc tttaacaagt tatcagagtt tggcagaggt
     1681 taagggctat caatttgaag tagaactatc accggttctc attgaaggaa atcctgttta
     1741 ttttttaaaa gctattaaga atattttaga taatgccttt cggtactcat gtaaagaagc
     1801 tattattcgt gtagtactac gccaaaatca attaataatc gaaaatcagg ttgaacgtgt
     1861 tttaagtgac gaggaattgg atcaagtctt tcgtccattc tatcgtccag actacagccg
     1921 tgataagaaa gatggtggta cagggattgg tttgtttatt gtacagaaaa tagtagaaaa
     1981 acacggcttt ccgtacactt tttatgcttc tgatacacaa actatgcgtt ttatgattca
     2041 tttgcaatca caaattacag cgaatagtaa taaattgata taggtaaaat t
//
LOCUS       DATUCG010000211         2047 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.214, whole genome shotgun sequence.
ACCESSION   DATUCG010000211 DATUCG010000000
VERSION     DATUCG010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.214"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(31..118)
                     /locus_tag="VCA20_002365"
     tRNA            complement(31..118)
                     /locus_tag="VCA20_002365"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(82..84),aa:Ser,seq:gga)
     gene            complement(121..468)
                     /gene="ssrA"
                     /locus_tag="VCA20_002366"
     tmRNA           complement(121..468)
                     /gene="ssrA"
                     /locus_tag="VCA20_002366"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            578..1171
                     /locus_tag="VCA20_002367"
     CDS             578..1171
                     /locus_tag="VCA20_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEP1836078.1"
                     /translation="MYKFWQKTIQVLSILTLIGTAIFLFWLYKIGILNDQNVLSDLIK
                     SQGALGSLSFLAIQIIQVVFPIIPGGVTTVVGFLVFHFWWGFFLNYLGISIGSIILFW
                     LARRYGKKFCLLFMSEDTFYKYESKIDNKRSYEIFFILCMLSPISPADIVVMITGLTS
                     MSYRKFIMITLLCRPFSVVAYSFFWIYGSQWLQQLLG"
     gene            1438..>2047
                     /locus_tag="VCA20_002368"
     CDS             1438..>2047
                     /locus_tag="VCA20_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HEP1836079.1"
                     /translation="MSYLQYTAKSHHLQWSVRQLSQICHHFYHNYCPDSFKHRRNVGL
                     VKVSDESILVLLLLQAELGMTSQRHFYTICHLFPCGQLLERSRFNRRSKQLIWLVQLI
                     RKAMSEMLPSDKLAIIDSFPLSLCQPVRNHRVTIFKGLADIGYNASKHLWFYGFKVHM
                     LVTLSGFILNYVVTPASVHDIKVVYELLEGCKQSVILGDLGYL"
BASE COUNT          574 a          424 c          371 g          678 t
ORIGIN      
        1 aacgactgac agtcgttctt caataacata cggaagacat gggattcgaa cccacgcacg
       61 ctgttacacg cctaccgcgt ttccaacacg gcctcttgag cctcttgagt aatcttccat
      121 atatggagct ggtgggagtc gaacccacgt ccaaacacct gctaacttat tcgtctacaa
      181 ccataggtta tgtcttgctt taacttgaac tcgatacata actcaaacct agaccaagcg
      241 agtcagtaaa tctcttttgg aactgcctga ctcaatccca acgtagcttg ctattttaag
      301 accaatcagc aaacacaagc aattcgcgac tggtcacgca ggcaggttat taagctgcta
      361 atgcgtaagt gtttgtattt tttgcagtta tatttaactg gcatttttac atctgccgat
      421 gagttgcaga acaagcctca taatgcctgt cgaatccgta acaaccccgt aggatttgat
      481 acaggaatca ttatagcaga attttttata aatagcaaaa ttattttaca ttttccttac
      541 aatttaaaaa aattttgaaa tatggtaaaa tgaaagtatg tataaatttt ggcaaaaaac
      601 cattcaagta ttaagtattt tgacccttat cgggactgct atttttctgt tttggctcta
      661 taagattggc attttgaacg accaaaatgt cttaagcgat ttaatcaaaa gccaaggtgc
      721 tttaggtagc ctctcctttt tagctattca gattattcaa gttgtatttc cgattattcc
      781 aggcggtgtt acgactgttg tgggtttcct cgtatttcat ttttggtggg gattctttct
      841 caattatctt ggaatttcaa tcggtagtat catccttttc tggttggctc gtcgctatgg
      901 aaagaaattt tgtctcctct tcatgtctga agatacgttt tacaaatatg aaagcaaaat
      961 tgataataaa cgtagttatg aaattttctt tattctttgc atgctttctc ccatttctcc
     1021 agctgacata gtggtgatga ttactggatt aacaagcatg agctatcgga aatttattat
     1081 gattacttta ctttgtcgcc ctttctcagt tgtggcctat agcttctttt ggatctatgg
     1141 cagccaatgg ttgcagcaat tgttaggata aaatcataac cacccgtgaa aacgggtggc
     1201 ttgtacaccg gctataagcc gtttctctcc agcgacgtct aaagacgttc gctgaacttc
     1261 gttcaggtta gtgctataat tacttgacta atgcctgtaa caacgggctt ttatcttgtt
     1321 ctaaaactac tatctgaaaa tgaatcttca cccgttgtgc tagttcattt caaaatacaa
     1381 taaaaagccc aaatggacta tactgtaagt gacgaaacat acaggaggtt agtccaaatg
     1441 agctacttac agtatactgc taaatctcat catttgcaat ggagtgtacg ccaattatct
     1501 caaatttgtc atcactttta ccacaactat tgcccggatt cctttaaaca tcggcgtaat
     1561 gttggcttag ttaaagtttc agatgaatca atccttgtct tacttttgtt acaagctgaa
     1621 ctaggtatga catcacagcg ccatttctac accatctgcc acctcttccc ttgcggtcag
     1681 ttactagaaa gaagccgttt caatcgtcgg tcaaaacaat tgatttggct agtccaatta
     1741 atccgaaaag ctatgagtga aatgcttcca tctgataaac tagccattat agatagtttt
     1801 cctttgtcac tttgccaacc tgttcgcaat cacagagtta ccatttttaa aggattagcc
     1861 gatattggct acaacgcctc taaacatctt tggttctacg gcttcaaagt tcatatgcta
     1921 gtgaccttat ctggttttat tctgaactat gttgtcacac ctgcctctgt tcacgacatc
     1981 aaggtggttt atgagctatt agaggggtgt aagcaatcgg ttatcttagg tgatttaggc
     2041 tacctaa
//
LOCUS       DATUCG010000212         2033 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.215, whole genome shotgun sequence.
ACCESSION   DATUCG010000212 DATUCG010000000
VERSION     DATUCG010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.215"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            <1..261
                     /locus_tag="VCA20_002369"
     CDS             <1..261
                     /locus_tag="VCA20_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEP1836080.1"
                     /translation="FLINASLLEEIVYRQLLWEKLTFPFVQIVVTSFLFVLSHGLNQL
                     GSWLMYSCLGLTLAVVRLKTDCMTAIALHLLWNSLAYVVTFL"
     gene            338..826
                     /locus_tag="VCA20_002370"
     CDS             338..826
                     /locus_tag="VCA20_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1836081.1"
                     /translation="MSSEQQERQAMQYVERSSLLTVRTLLKLLEWSARQALAQDSAYK
                     IGVQKLEELLQSPYAIEAIDVSKDILDKPIDVEKFKELMESEKLPIAISWQKDYLYFY
                     AKDKTLLDHHLNELVKKLVSNPEKLDGLTFDKTLDQEIELAKEKIRETEPSAVKTKEV
                     TL"
     gene            826..>2033
                     /locus_tag="VCA20_002371"
     CDS             826..>2033
                     /locus_tag="VCA20_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HEP1836082.1"
                     /translation="MYSRRKALVFGLMGLAFGYFCHRLTLLYDSLTHAPPMERFSYLL
                     GEGLTQVFNPLWLFSFTQKSLLAFILGVLTMTLVYLYVSTGQKVYREGEEYGSARFGS
                     SKEKRLFYSKNPFNDTILARDVRLTLLEKKKPQFDRNKNLIVIGGSGAGKTFRFVKPN
                     LIQLNCSNIVVDPKDHLAEKTGKLFLENGYQVKVLDLVNMTNSDGFNPFRYVETENDL
                     NRMLTVYFNNTRGSGSRSDPFWDEASMTLVRAIASYLVDFYNPPGSSKQEQEARRKRG
                     RYPAFSEIGKLIKLLSKGDNQDKSVLEVLFEDYAKKYGHENFTMRNWADFQNYKDKTL
                     DSVIAVTTAKFALFNIQSVIDLTQRDTMDLKTWGTQKTMVYLVIPDNDTTFRFLSALF
                     FSTVFSTLTR"
BASE COUNT          599 a          382 c          442 g          610 t
ORIGIN      
        1 tttctaataa atgcaagtct ccttgaggag attgtttata ggcaattgct gtgggaaaaa
       61 ttgacattcc cttttgtaca aatagtcgtg accagttttc tctttgttct atcccatgga
      121 ctcaatcagt tagggagttg gctcatgtat agctgtcttg gcttgacctt ggctgttgtt
      181 cgattgaaaa ctgattgtat gacggcaatc gccttacatt tactttggaa tagtttggct
      241 tatgtcgtaa ctttcttgtg ataccaaaat caagagtgct tccgtattat ggaaacacct
      301 tatgtttgat agggaatcga aaaaagagga ggtcaccatg tcatcagaac aacaagaacg
      361 tcaagcgatg caatacgtgg aaagaagtag tttattgaca gttagaaccc tcttaaagct
      421 cttagagtgg tcggctaggc aagccttagc tcaagattca gcttataaga ttggggttca
      481 aaaactggaa gaactccttc aaagccctta cgcaattgaa gcgatagatg ttagtaaaga
      541 catactggat aagccaatcg atgtggagaa atttaaggag ttgatggagt cagaaaaact
      601 gccaatcgca attagttggc aaaaagacta tctctacttt tatgccaagg ataagacctt
      661 gcttgatcat cacttgaacg aactggtgaa gaaactggtg tctaacccag aaaagttaga
      721 tggtctaact tttgataaaa ccttggacca agagatagaa ctggccaaag aaaaaataag
      781 agagacagaa ccttcggcag tcaaaaccaa ggaggtgacc ttgtaatgta ttcaagacga
      841 aaagctttag tctttggact catggggctc gcctttggct atttctgtca tcgtctaacc
      901 ctactctatg atagtttaac ccatgcccca cctatggaac gtttttcata cctcttagga
      961 gaggggttaa ctcaggtttt caatccttta tggctatttt cctttactca aaaatctctt
     1021 cttgccttta tccttggggt tctaacgatg acactagtct atctttatgt atcgacagga
     1081 cagaaggtct atcgagaagg ggaagaatac ggttctgcac gatttgggag cagtaaggaa
     1141 aagcggttgt tttacagtaa gaatcctttt aatgacacga ttttggctcg tgatgttcgt
     1201 ctaaccttgt tggaaaagaa gaaaccccag tttgaccgaa ataaaaattt gattgtcatt
     1261 gggggttctg gggcaggcaa gacctttcgc tttgtgaagc ccaaccttat ccaacttaat
     1321 tgttccaata ttgtcgtaga tccgaaagac catttggctg agaagacagg taaactcttt
     1381 ttagaaaacg gttatcaggt caaggtttta gacttggtta atatgaccaa ttcggacggt
     1441 tttaatccct ttcgttatgt agaaacagag aatgatttga accgcatgtt aacggtctat
     1501 tttaataata cccgtggatc tggttctcgc agtgatccat tttgggacga ggcttccatg
     1561 acattggtga gagctattgc ctcttacttg gtggattttt acaatccacc aggaagttct
     1621 aagcaagagc aggaagcaag acgtaagcgt ggccgttatc cagccttttc tgagattggg
     1681 aaactcatca aactcttatc aaagggagac aatcaggaca aaagtgttct tgaagtcctg
     1741 tttgaagatt atgctaaaaa atatggtcac gaaaacttta ccatgcgaaa ctgggcggat
     1801 ttccaaaact ataaggataa gaccttggat tcggtgattg cggtcacaac agctaaattt
     1861 gcccttttta atatccaatc ggtgattgat ttgacgcaaa gagacactat ggatttgaaa
     1921 acgtggggca ctcaaaagac tatggtctat cttgttattc cagacaatga tacgaccttt
     1981 cgtttcctat ctgctttatt tttctctacg gttttctcca ctttgaccag aca
//
LOCUS       DATUCG010000213         1984 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.216, whole genome shotgun sequence.
ACCESSION   DATUCG010000213 DATUCG010000000
VERSION     DATUCG010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.216"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            <1..1593
                     /locus_tag="VCA20_002372"
     CDS             <1..1593
                     /locus_tag="VCA20_002372"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012948.1,HMM:NF018196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEP1836083.1"
                     /translation="PNTGHLFVNEKTGEVVTSSSNGATRIHQMAFSTDSDGTGTVYVH
                     MYSEDGRLLMWDSLVPRVVEELKFDRKNWWGKLDRTYSIKYASVITINGTFPVVSVST
                     SDASDGWFDELYTNYERALGHTDGRYMGYGWFVPRLIDKKTEYVVKDSNKVLATVTQT
                     EMSGYKFTTAAPETITADGVTYKLIKDSGVNTSGLINPLVMAGDVTAVGTDKGYFIRY
                     LMKDEAGQNIEWELLDPNNEVATTTDGKPARGTLRYGEDKKTYATVRVKSTNTDTTFY
                     INNPYFPGGRTVYYYEVVPTSEVPKGSVTVKHVTTTGEVLKKETPVKTDEPVGTPYET
                     QPEDKIVTDNGRTFVYRGLKEDSADPKGEVKEGTQKVTFVYEEVKGNVTVEHVTTDGK
                     VLKAETPVFSENQSTGTAYETKNETELKDEAGNVYVFKEHKADSAPVSGEVTEKAQKV
                     VYVYELKPVTPTPTPEVSKPKSTTPATPTQDSSIPTSVVSTNKAQLPNTGEDNSAAFA
                     TLGTAMLVAAFALNGKRRQDEN"
BASE COUNT          663 a          360 c          427 g          534 t
ORIGIN      
        1 ccaaatacgg ggcatctttt tgttaatgaa aaaactggtg aggtggtgac atcatcttct
       61 aacggagcta ctcgtatcca tcaaatggct ttcagtacag atagtgacgg cacaggaacc
      121 gtttatgtac atatgtattc agaagatggt cgcttactca tgtgggattc tttagtacca
      181 cgtgttgttg aggaactaaa atttgataga aaaaattggt ggggaaaact tgatcgtaca
      241 tattctatca agtacgcatc tgttattaca atcaacggga cgtttccggt cgtatctgta
      301 tctacttcag atgcatcaga tggatggttt gatgaattgt ataccaatta cgaaagggca
      361 ctgggacata ccgatggtcg ttatatgggc tatggctggt tcgttcctag attaattgac
      421 aagaagacag aatatgttgt caaagattct aacaaagtgt tagcgactgt gacacaaaca
      481 gaaatgtcag ggtacaaatt tacaactgca gcaccagaaa caattacagc agacggtgtg
      541 acttataagt taatcaaaga tagcggtgtt aatacaagtg gtcttatcaa tcctctcgta
      601 atggctggtg atgtgactgc tgtgggtaca gataaaggtt atttcatcag atatctgatg
      661 aaagacgaag caggtcaaaa tattgagtgg gaacttttgg atcctaataa tgaggttgct
      721 acaacaactg acggtaaacc tgctagaggt actctacgtt atggtgaaga taaaaaaact
      781 tatgcaactg ttcgtgttaa atctactaac acagatacca ctttctacat taataatcca
      841 tatttcccag gtgggagaac agtctactat tatgaagttg ttccaacttc ggaagttcct
      901 aaaggtagtg tcacggtaaa acacgttaca acaactggtg aagttctgaa gaaggaaacc
      961 ccagtcaaga cagatgaacc agttggtaca ccatatgaga cccaaccaga agacaagatt
     1021 gtgacagaca acggtcgtac gtttgtttat agaggtctca aggaagactc tgcggatcca
     1081 aaaggtgaag tcaaagaggg tactcaaaaa gtaaccttcg tttacgaaga agtcaaaggt
     1141 aatgtcacag tcgagcatgt aacgacagac ggtaaagttc ttaaagcaga aactccagtc
     1201 tttagcgaaa accagtcaac tggcacagct tatgagacga agaatgaaac tgaattgaaa
     1261 gacgaagcag gcaatgttta tgtcttcaag gaacacaaag cagattcagc cccagtttca
     1321 ggcgaggtta ctgagaaagc tcaaaaagtt gtttacgttt acgagttgaa accagtcact
     1381 ccgactccaa caccagaagt ttcaaaacca aaatctacta cacctgctac accaacgcaa
     1441 gatagcagca tcccaacatc agtagtttct acaaataaag cgcaattgcc gaataccggt
     1501 gaagataact cggcagcttt tgcaacacta ggtacagcta tgttggtggc tgcatttgca
     1561 ctcaacggaa aacgtcgtca agacgagaac taataaagcg ttttgcaatg tatctttaaa
     1621 agagggtatc gttttttacc atcaagctct ttgagtggca agtagaatga gtagcttttt
     1681 gaagcctcct gtcaaccgta gcgggcaact tatagattga ttcaaaagtc taggggaaaa
     1741 ttagtcctta catattaaaa aacgagcaat tccaatttgg agttgctcgt tttccattct
     1801 ataaaactta tttttggtgg tttagttgaa atgtttaaaa aaggtcaaag gaaagagaat
     1861 tccgatatta gggatgtgtc gagggtcaaa ttattaatta tagtctttta acccgttgtg
     1921 ctagttaatt ctaggtaaat aaaaacactt tgcgtatcat aaactcaaaa caaccacgaa
     1981 atga
//
LOCUS       DATUCG010000214         1984 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.217, whole genome shotgun sequence.
ACCESSION   DATUCG010000214 DATUCG010000000
VERSION     DATUCG010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.217"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            563..1882
                     /gene="ltrA"
                     /locus_tag="VCA20_002373"
     CDS             563..1882
                     /gene="ltrA"
                     /locus_tag="VCA20_002373"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000561482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEP1836084.1"
                     /translation="MIAQKAINIFEKVQVFQRKIYLSTKADNKRKFGVLYDKVYRKDV
                     LKVAWNYVKRNKGSAGIDDFTIEEIEAYGVQKFLDEIEDQLRNKKYQPKAVKRVYIPK
                     ANGQKRPLGIPTVKDRVVQTAVKIVIEPIFEADFQEFSYGFRPKRSANQAIREIYKYL
                     NYGCEWVIDADLKGYFDTIPHDKLLLLVKERVTDKSIIKLLSLWLEAGIMEDNQVRSN
                     ILGTPQGGVISPLLANIYLNALDRYWKNNRFEGRGHDAHLIRYADDFVILCSNNPKKY
                     YQYAKQRIDKLGLTLNEDKTRIVHATEGFDFLGYTLRKAKSHKSGKYKTYYYPSRKSM
                     KSIKGKVKDVIQTGQHLNLPDVIERLNPMLRGWANYFKAGNSKQHFKSIDNYVTYNLT
                     IMLRKKHKKSGKGWREHPPSWYYNYFGLVCLRKMSTNINDDSQRYGR"
BASE COUNT          696 a          317 c          457 g          514 t
ORIGIN      
        1 ctcaatctaa caggtgaaag tcctgtccta acaattaacc agttcggtta ggtagctatg
       61 gcatacgtat gcggagtaat ccaaatgcgg aagcaatgcc gactaacgtt gtccccggta
      121 ggctgatagt tattggtctt atctcagtca gtgggagcga gcgaacctac agtccgtaac
      181 gaaagtgaac ttccgataag tgtcgttaag gtcattccaa cagttataac tgagagagtt
      241 ggaaatagag ttgtcgagcc tttagaacgg gggcgaagac tggtattgtt gtcagttcta
      301 actggtatat actggcaata aagctctcgg catatagaag atggaatgta gggaaagatg
      361 agcgaggaac ggaggagacc ctctcctatc atacaaaccc atgtatgtaa agttagatat
      421 aaggttgagc gctgaaattc taactgacgt aggaacaggg agtcggagag gttcatagta
      481 ccaataatca cacgagacaa cagaactcgg tgtagggaag gagccttgcc tttatcggtc
      541 ttaccgatga aagaggaact agatgattgc tcagaaagct ataaacatct ttgaaaaggt
      601 tcaagtattt cagaggaaga tatatctatc gaccaaggca gataataagc gaaagtttgg
      661 ggtcttatat gataaagttt atcgtaaaga cgtcctaaaa gttgcgtgga actatgtcaa
      721 aaggaataaa ggttcagcag gcattgatga cttcacgatt gaagaaattg aagcctatgg
      781 tgtacagaaa ttcctcgatg aaatagaaga ccaactgaga aataagaagt accaaccaaa
      841 agcagtaaaa cgagtttata ttccgaaagc taatggtcaa aagagaccgt tgggaattcc
      901 gacagtaaag gatagagtag ttcaaacagc tgtaaaaata gttatagagc cgatattcga
      961 agctgacttt caagaatttt cttatggctt tagacctaaa cgaagtgcta accaagctat
     1021 aagagagata tataaatatc tcaattatgg ttgtgagtgg gttattgatg ccgacttaaa
     1081 aggttacttt gacacgatac ctcacgataa gttactactt ttagttaagg aacgagtaac
     1141 tgataagtct attatcaagt tacttagttt atggctagaa gcaggtatca tggaagacaa
     1201 tcaagtgaga agtaatattt taggtactcc acaaggaggt gtcatatctc cattgttagc
     1261 gaatatctac ttaaatgctt tagacaggta ttggaagaat aatcgttttg aaggaagagg
     1321 acatgacgcc cacttgatac gctatgcgga cgactttgtt attttatgtt caaataatcc
     1381 aaagaaatac taccagtatg cgaaacagcg tattgataag ttaggattaa cactgaatga
     1441 agataaaaca agaatagtcc atgcgacgga gggttttgat tttctaggtt atacgcttag
     1501 gaaagctaag tcacataaaa gtggtaagta caaaacctac tactatcctt caagaaaatc
     1561 tatgaaatca atcaaaggga aagtaaagga tgttatccaa acaggacaac acctaaacct
     1621 tcctgatgtc atagaaagat tgaatccgat gttaagagga tgggctaatt atttcaaagc
     1681 tggcaactcc aaacaacatt tcaagagtat agataactat gtcacatata atctaacgat
     1741 tatgcttaga aagaagcaca agaagtctgg aaagggatgg agggaacatc cgccatcatg
     1801 gtattacaac tactttggac tagtctgttt aaggaagatg agtaccaata tcaatgatga
     1861 cagtcagaga tatggtagat aacttgtgaa tggctataat gtcgaagaaa gaagataatg
     1921 ggaaagccgt gtaagggaaa accttacgca cggtttgacg aggggtttct gaggagataa
     1981 tttc
//
LOCUS       DATUCG010000215         1914 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.218, whole genome shotgun sequence.
ACCESSION   DATUCG010000215 DATUCG010000000
VERSION     DATUCG010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.218"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            <1..828
                     /locus_tag="VCA20_002374"
     CDS             <1..828
                     /locus_tag="VCA20_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase SNF2"
                     /protein_id="HEP1836085.1"
                     /translation="KYITQIMTSKDPVRSAEDIDEQTMTASDFKALATGNPYLKLKME
                     LENELTVLENQKRAFNRSKDEYRHTISYSEKHLPIMEKRLSQYDKDIAQSLTTKSQDF
                     VMRFDNQAMDNRAEAGDYLRKLITYNRSETKEVRTLASFRGFDLKMTTRGPSEPLPET
                     VSLMIVGDNQYTVALDLKSDVGTIQRISNAIDHIMDDKEKTQELVKDLKDKLRVAKVE
                     VEKVFPKEEEYQLVKSKYDVLAPLVEKEAEIEEIDAALAKFSEDTTPQKKQQIALEI"
     gene            899..1198
                     /locus_tag="VCA20_002375"
     CDS             899..1198
                     /locus_tag="VCA20_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000194854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1836086.1"
                     /translation="MTKTVEEMRFQLEVWLAQGYTSPEDRANYQTLKEQYEDETFDYS
                     FSRREITGQLELIITSRENDFPSLDEVTKAEYLDLVAQLDDLDKRQADYYRKQLA"
     gene            1212..1502
                     /locus_tag="VCA20_002376"
     CDS             1212..1502
                     /locus_tag="VCA20_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000893084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1836087.1"
                     /translation="MLEQILQSLLIIAAIGLMLLVLYQIVKVSGALFLIGLISGLVFI
                     EIYGIYLFFTERYLYSEDLFTNGIWSFTGFYIALNLFLVFSIIMKWWRNRIV"
     gene            1598..>1914
                     /locus_tag="VCA20_002377"
     CDS             1598..>1914
                     /locus_tag="VCA20_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEP1836088.1"
                     /translation="MLNKVKARFLIGVGGLIAVSFMVMIGYTIGSQSVSKQTENQIRA
                     EANKLVTKEKQEEKATVLSSELVKEFLTQYYTKVQLGENNTRIEPYMTDSAFSEEEAR
                     QNKA"
BASE COUNT          660 a          330 c          407 g          517 t
ORIGIN      
        1 aagtatatca cccagataat gacctcaaaa gatcctgtga gatcagctga agacattgat
       61 gaacaaacca tgaccgcctc agactttaag gccttggcaa ctggaaaccc ttatctcaaa
      121 ctcaaaatgg agttggaaaa tgaactgaca gttttagaga atcaaaaacg agcctttaat
      181 cgctccaaag acgagtatcg ccataccatt tcctatagtg aaaagcacct ccctataatg
      241 gaaaaacggt tgagtcaata tgataaggat attgcccaat ctttgacaac aaagtcgcaa
      301 gattttgtca tgcgatttga caatcaagca atggataatc gtgctgaagc aggggattat
      361 ctgcgtaaac tcatcaccta taaccgctca gaaactaagg aagttcggac tctggccagc
      421 tttagaggat ttgatttgaa aatgacaaca cgtggtccta gtgagccctt gccagagact
      481 gtctctttaa tgattgtagg agataatcag tatactgtcg cccttgattt gaaatcagac
      541 gttggaacta ttcaacggat tagtaatgcc attgaccata tcatggatga caaagaaaag
      601 acgcaagagc tggtaaaaga tttaaaagat aaactacgag tagccaaagt agaagttgag
      661 aaagtctttc ccaaggaaga ggaatatcag cttgtaaagt ctaagtatga tgttttggct
      721 cccttggtag aaaaagaagc agagattgag gagatagatg cagctttggc caagtttagt
      781 gaagatacaa caccccaaaa gaagcaacaa atagcactcg agatataaga aaaaaggctg
      841 acaaatattt ttgaacgcgg taaaatgaag tcaagaaatc acaaaaggag aacagaccat
      901 gaccaaaaca gtagaagaaa tgcgttttca acttgaagta tggttggcac aaggctatac
      961 aagtccagaa gatagggcaa actaccaaac tttaaaggaa cagtatgaag atgagacctt
     1021 tgattacagt ttttcaaggc gtgaaattac tggacagctg gaactcatca tcacaagtcg
     1081 tgagaatgat tttcctagct tagatgaggt gacgaaggca gaataccttg atttggttgc
     1141 ccaacttgat gatttagaca agagacaggc tgactactat cgcaagcaat tagcctagaa
     1201 agaggtgtaa gatgttggag caaattctac aaagcctttt gattatcgca gcaataggac
     1261 tgatgttgct cgtcctttat cagattgtga aagtttcagg tgctttattt cttatcggac
     1321 tcatcagtgg tttagtcttt attgaaatct atggaattta cctcttcttt actgagagat
     1381 atctctattc agaagattta ttcaccaatg gtatttggag ttttacagga ttttatattg
     1441 ctttgaatct atttcttgtt tttagcatta ttatgaagtg gtggagaaat agaatagttt
     1501 aacacggagg attaccactg ggtagtcctt tttcttgtga taaaatctcc aagtcattct
     1561 tgttatgatg tgcctatcac taataaagga gtaagctatg ttaaataaag tgaaagctcg
     1621 atttctgatt ggagtaggag gtttaatcgc agtcagtttt atggtcatga ttggctatac
     1681 gattggctca caatccgttt caaagcaaac tgaaaaccaa atccgagcag aggctaataa
     1741 acttgtgaca aaggaaaaac aggaagaaaa agctactgtc ttatcaagtg agcttgtcaa
     1801 agaatttctc acacaatatt atacaaaggt tcagctagga gaaaataaca ctcgtatcga
     1861 accctacatg acggattcgg cattttcaga agaggaagca aggcagaata aagc
//
LOCUS       DATUCG010000216         1813 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.219, whole genome shotgun sequence.
ACCESSION   DATUCG010000216 DATUCG010000000
VERSION     DATUCG010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.219"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(1..517)
                     /locus_tag="VCA20_002378"
                     /pseudo
     CDS             complement(1..517)
                     /locus_tag="VCA20_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636793.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
     gene            complement(518..>1813)
                     /locus_tag="VCA20_002379"
     CDS             complement(518..>1813)
                     /locus_tag="VCA20_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1836089.1"
                     /translation="KMIGEDIEALEVFFAHTIAPICTGILVALGLTVYFGLSSWGLAF
                     VALVTYALLAIAIPKQFAKQLQPLLKEQNASRKGYVSYFIESLKSMKDLMQFQQTDER
                     FANLTNKSQEANGQERKVAQTNFMQYAVSFLVVGLSIMGFAWLTFDLVGSQSLGLATG
                     VALLVSFTSSFAPFLELSRLSLGFKRAMNAGRNIYALLDEKEAERTGDLVEVSVKDIV
                     IEQLDFDYDDRETGLYRNLSVRFEQGGIIGLVGESGAGKSTLMKLIMRWYDWQQGQIR
                     LSGLDSRQVDKAHLQGAFAYVPQVPQIFRQTIRENLVLGRTDVSDETIMDLAEKCHMK
                     ERILAAPQGLDTLVEASDFSAGEGQRLELMRALLKNADCYIFDEPTSNLDSLNEARFI
                     QLVKEHCQGMVFLISHRSSTMTCADTIFRLEDGQLVKEK"
BASE COUNT          544 a          465 c          374 g          430 t
ORIGIN      
        1 agataatggt cgttgacaac catagcacat tctctggagt gaaatcaagc atgacacgag
       61 cgatgtattc tgccgatttt ccacgtgcca aaagattggc ctcataggca tctcgcatga
      121 actacataag aataataggt cccaagtttc caaaagagaa aatgatatag ctaaaaagat
      181 tggtccattt gtgtttatag tgacctgact tcgctaccat ttctgccaaa aaactaaagg
      241 tcaaacttgg gagaaaggcc gctgtcatat agcctgatag gaaaaagaaa caggccatta
      301 ctgctccaac caaactaatg gagccaaact taccaacctt ggcaatcaag agcatataga
      361 ctggaccagc caagagggct gcaaaagctg gagcatacat catattaccc gagcgatcaa
      421 aaaccaagct caacaaggtt cctagaccaa cacaaacaaa gtagagggca gcaaaagccc
      481 ctgttaccat cagatctttt acttgtaatt tcttcattta tttttccttt actaattgtc
      541 cgtcttccaa gcggaaaatg gtatccgcac aggtcatggt cgagcttcta tgagaaatca
      601 agaagaccat gccctgacag tgttccttaa ccagttggat aaagcgggct tcattgagcg
      661 aatctaggtt actagttggc tcatcaaaga tatagcagtc cgcatttttg agtagggccc
      721 gcatgagttc caagcgttgt ccctctcctg ctgagaagtc actggcttcc acaagcgtat
      781 ccaagccctg cggtgctgcc aaaatccgtt ctttcatgtg gcatttttct gccaagtcca
      841 tgatggtttc atccgacaca tccgtacgac ccaagacaag attttcacga attgtctggc
      901 gaaagatttg tggtacctga ggtacatagg caaatgcccc ctgcaaatga gccttatcta
      961 cctgccgact atccaagcca gacaggcgga tttgaccttg ctgccaatca taccaccgca
     1021 tgatcaattt cattaaggtg gatttacctg caccggactc accgactaag ccaataatac
     1081 caccctgttc aaaccgaaca gacaggttcc gatagagccc ggtttctcga tcatcatagt
     1141 cgaagtccag ttgctcaata acaatatctt tcacgctgac ctcgaccaag tcacctgttc
     1201 gctctgcttc tttttcatcc aaaagggcat agatattgcg accagcattc atagcccgct
     1261 tgaaaccaag cgacagacgg cttagttcca agaagggagc aaaggagctg gtaaaggaaa
     1321 ctaggagggc tactccagtt gccaagccca aactctgact acccaccaag tcgaaggtca
     1381 gccaggcaaa gcccatgata gacaaaccaa ccaccaaaaa ggaaacagca tactgcataa
     1441 agttggtttg agcgaccttt ctctcctgac cattggcctc ttggcttttg ttcgtcagat
     1501 tggcaaagcg ttcatctgtc tgctggaact gcatcaggtc cttcatgctc ttaagacttt
     1561 caataaagta agacacatag cccttacggc tagcattttg ctccttgagc aagggctgca
     1621 gctgcttggc aaactgcttt ggaatagcaa tcgccaaaag ggcataagtc accagagcca
     1681 caaaagccag accccagctg gatagaccga aatagactgt caaaccaaga gccaccagaa
     1741 tccctgtaca aatgggtgca atagtatggg caaagaaaac ctccaaggcc tcaatgtcct
     1801 ccccaatcat ctt
//
LOCUS       DATUCG010000217         1772 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.220, whole genome shotgun sequence.
ACCESSION   DATUCG010000217 DATUCG010000000
VERSION     DATUCG010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.220"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(1..289)
                     /locus_tag="VCA20_002380"
                     /pseudo
     CDS             complement(1..289)
                     /locus_tag="VCA20_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001871253.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            479..1432
                     /locus_tag="VCA20_002381"
     CDS             479..1432
                     /locus_tag="VCA20_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_053338614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HEP1836090.1"
                     /translation="MTSISQNLRYLLHTLETRYHAVKTYRNGASVAFICRRYKVSKAS
                     LMRWNKRFDGTKESLKDRSHRLLTPHPKAHTQQELTWIKNCIRRNPKATLIEIFYKLK
                     TNKGYDRHPCSLFRILRKLEFFNSPKTKKKTYVPKPYDTPTNLGIKWQIDVKYVPTHC
                     YTVKLPDKFYQYTVIDEASRERFIYPFKEQSSYSTVQFLKMAIKHFGYKPKIIQTDNG
                     FEFTHFKETKQVHPLDLLCQELGIEHKLIRPRTPRHNGKVERSHRNDNQRFYKHLTFY
                     SYDDLIKQMKTYLYRSNRLPMQTLGWKSPIDIRKALLGTSS"
BASE COUNT          572 a          402 c          301 g          497 t
ORIGIN      
        1 gagcaatcat tcaacttgag ataagaacac ttttgacgat tggtttcata gacaccttgt
       61 ccactatcag cgaaataggt gctgaaatag gttcgtttcc cattttatcc tgtacctgat
      121 taactgaacc acgtttgatt tctcgacaga ttgtagaccg gtgacgacct agaagccgag
      181 cgatttcaat tggtttcttt cccattttaa gataagcaac aatttcacca cgttctgtgg
      241 ctgagagatg agaataggat gagtttctgg tagaatgagt gttggacatg ttcatctgct
      301 gtctatactg agttggggaa ttctagtata tcagacgaac atatcttttt tgttgcactt
      361 cattttacaa cgcgggaaaa gtgtagcgtt acaattgatg tgagacttct agaagtcaaa
      421 aagaagattc cttgatatga taagagtacc acctactcac caaaaaagga gtcttctcat
      481 gactagtata tcacaaaatc ttcgctattt actacacact ctggaaacac gttaccacgc
      541 ggttaaaacc taccgaaatg gtgcttctgt cgcctttatc tgtcgccgat ataaagtctc
      601 aaaagcctct ctcatgcgct ggaacaagcg attcgacgga accaaagaat cgctgaaaga
      661 ccgttctcat cgccttctaa caccgcatcc aaaagcccat acccagcagg aactgacctg
      721 gatcaaaaat tgcatcagaa gaaatccaaa agcaacactc atagaaatct tctacaagct
      781 caaaaccaat aaaggatatg accgccatcc ctgctctctc tttcgaatct tgagaaagct
      841 tgagttcttc aattccccta aaacaaagaa aaaaacttat gtaccgaaac catatgacac
      901 tccgactaac ctcggtatca agtggcagat agatgtcaaa tacgtaccga ctcactgcta
      961 cacagtaaaa ctacccgata agttttacca gtataccgtc attgacgaag ccagtcgaga
     1021 acgttttatc tacccattca aagagcagtc gtcctactca actgttcaat ttctaaaaat
     1081 ggctatcaaa cactttggat acaaaccaaa aatcattcaa acggataatg gttttgagtt
     1141 tacccatttc aaagaaacca aacaagtaca ccctctagat ttactctgtc aggaacttgg
     1201 cattgagcac aaactgattc gtcctcgaac tccaaggcat aatggaaagg tggaacgcag
     1261 tcatcgaaat gacaatcaac gtttctacaa gcacttgacc ttctattcct atgacgatct
     1321 tatcaagcag atgaaaacct atctgtaccg ctccaatcga ctccctatgc aaactttagg
     1381 ttggaaatct cctatcgata tcagaaaagc tttactagga actagctcct agtaaagctt
     1441 aacaaaagga taacttcaaa atcacctttt tggtctcaca tcattgacaa aggtacatat
     1501 tttcactttc tttttttaaa gtatctatac tcatacccgt tgtgctagtt aatcaagcgt
     1561 aagcaaggga atattttaaa ctgaagccaa attcagtatg actgctttta agcaaaagag
     1621 aatagataag taatattgcc tctgtcttaa atttgagcgc ctgaatgttt cggcaacgta
     1681 acccctccat tccaaactgc tctaaactag aaaatacggt ctccactggt ttcctcaatc
     1741 gacttatttt gtaattttct agccatgagt aa
//
LOCUS       DATUCG010000218         1682 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.221, whole genome shotgun sequence.
ACCESSION   DATUCG010000218 DATUCG010000000
VERSION     DATUCG010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.221"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            218..1138
                     /locus_tag="VCA20_002382"
     CDS             218..1138
                     /locus_tag="VCA20_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEP1836091.1"
                     /translation="MIHLICPNPALDRTLLVEKIEKNIPLRPTEVRDYPGGKSFNVAY
                     ALRENGVTDYTIHTILGGQIGRYIQELNADGGNALCVVENNQNTRTCNIYVETSTGDV
                     VLFYEKGFELTEDLLNQFTQQIENSLQAGDILVFSGSLMKGMPDDYIQEFIEKYPEVL
                     TIVDTSGPALRAAYQARPALIKINNEELKDIYPELDEESPEDILRILKDVTPHENIII
                     TMGDKGSLAKIGQRFFRIQSPKKETRNPIAAGDFYLGLLVKGISQGQAPEIFLKEAAA
                     FATANCLNYFPEVEAEQFAEIAGKVVLEQV"
     gene            complement(1124..1366)
                     /locus_tag="VCA20_002383"
                     /pseudo
     CDS             complement(1124..1366)
                     /locus_tag="VCA20_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088453.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1437..1652
                     /locus_tag="VCA20_002384"
     CDS             1437..1652
                     /locus_tag="VCA20_002384"
                     /inference="COORDINATES: protein motif:HMM:NF012948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEP1836092.1"
                     /translation="METAKGESFKAVALPTIDPATVKATETKAAEKKATSTASEKLPE
                     TGEVMTVLSLVGGLGLLGSAALLKKRK"
BASE COUNT          535 a          335 c          366 g          446 t
ORIGIN      
        1 aacaaatcgc tcatataaag cgagaacttg ggtaaagatt tttgaaaaaa ataacttgtg
       61 tttgattggc atttgcgaga taataatgtt ggctttgcat atcattccct catttcgtgg
      121 ttgttttgag tttatgatac gcaaagtgtt tttatttaac tagaattaac tagcacaacg
      181 ggtataataa tataaataaa aagaaaagga gaaccccatg attcacttga tttgtcccaa
      241 tccagctcta gaccgcacgc ttcttgtgga aaagattgaa aagaatattc ccctgagacc
      301 gacggaagtg agggattatc ctggtggaaa aagtttcaac gtagcctatg cacttcgaga
      361 aaatggagtg acagactaca ctatccatac cattttaggt gggcagattg gtcgctatat
      421 tcaggagctt aatgctgatg gtggaaatgc cctgtgcgtg gttgaaaata atcagaacac
      481 aagaacctgc aatatttacg tagaaaccag cacaggcgat gtagtactat tttatgaaaa
      541 aggttttgaa ctaacagagg acttgctcaa ccaattcact cagcagattg aaaatagcct
      601 gcaagctggc gacatcctag tcttttcagg cagtctgatg aaggggatgc cggatgacta
      661 tattcaggag tttattgaaa aatacccaga ggttctgacc atagtggaca ccagtggacc
      721 agctttacgt gcggcttatc aagctagacc ggcccttatc aaaatcaata atgaggagct
      781 gaaagatatt tatccagagc tggatgaaga aagtcctgag gatattttac gaatactgaa
      841 agatgtaacc cctcatgaga atatcatcat taccatggga gacaagggga gtctagctaa
      901 gattggtcag cgcttcttcc gtattcagtc tccaaagaaa gaaacgcgta atcctattgc
      961 cgcaggggat ttctacctgg gcttgctagt caaaggaatc agtcaaggtc aagccccaga
     1021 aatctttttg aaagaagcgg cagcctttgc aacagccaat tgtttgaatt attttccaga
     1081 ggttgaagca gagcagtttg cagagatagc gggtaaagtt gttctagagc aggtttaaaa
     1141 atatttcctc agttcgtctc atggccatca ctttccaatc gttatgttct tcagcccctg
     1201 ccatgttttg tcgaaatggc gtccatagat ggtaaccttc ttgttctaag tccttcttga
     1261 gttcactgct caggtagcct aaatcaccta agataaccga ttgcttacac ccctctaata
     1321 gctcataaac caccttgata tcatggacag aggctggcgt gacaacagac cttccaacaa
     1381 gcgaaggtgc tttagaaaca gctaaaggta caggtgtaac tgcaacatta ccagctttgg
     1441 aaactgccaa aggtgaaagc tttaaggcag tagctttgcc aacaattgac cctgcaacag
     1501 ttaaagcaac tgaaacaaaa gcagcagaga agaaagcaac ttcaacagca tcagaaaaac
     1561 ttccagaaac tggtgaagtg atgacagttc tttcgcttgt aggtggtcta ggtcttcttg
     1621 gctcagcagc tttgctcaag aaaagaaaat aatctacata caaaaaagga cctaaatggt
     1681 cc
//
LOCUS       DATUCG010000219         1673 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.222, whole genome shotgun sequence.
ACCESSION   DATUCG010000219 DATUCG010000000
VERSION     DATUCG010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.222"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            <1..745
                     /locus_tag="VCA20_002385"
     CDS             <1..745
                     /locus_tag="VCA20_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HEP1836093.1"
                     /translation="VAFGGVSNLGGLLSPSTGTNPYQSSQVTTTGTDVSDADAEFMSK
                     VLGSTENFWIQEFKANGLTYNAPTIVFYTNQTQTGCGVGSAQAGPFYCSADRKIYIDT
                     SFYQELSSKYKAAGDFAMAYVIAHEVGHHVQNELEVLGAYHQKRAQLSDKEGNALNVR
                     LELQADYYAGAWAKYVDGQGILDAGDIDEAMNAAHAVGDDTLQQEAYGRTVPDSFTHG
                     TSEQRKKWFNLGYTYGDLAHADTFSVEKP"
     gene            complement(954..1505)
                     /locus_tag="VCA20_002386"
     CDS             complement(954..1505)
                     /locus_tag="VCA20_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEP1836094.1"
                     /translation="MIVFLVICIFWVRIYFLKISIKNEKRILKNGGSEFGVQNTKLLT
                     VVHILFYLSCLVEAVVRHTKFDTLSSLGLIFLLFSMGMLFWVTRLLGDIWTVKLMLVK
                     DHRFVDHWLFRVVKHPNYFLNIIPELIGLALLCHATYSFLILFPVYMVILYRRIHEEN
                     NLLKTVIIPNGTVQSAAEITGNN"
BASE COUNT          550 a          351 c          279 g          493 t
ORIGIN      
        1 agtagccttc ggaggcgtgt caaaccttgg tgggttactt tccccatcaa cagggactaa
       61 tccttaccaa tcgagtcaag tcacaacgac tggtacagat gtgtctgatg cagatgctga
      121 attcatgagt aaggttttag gatcgacaga aaatttttgg atccaagaat ttaaagccaa
      181 tggtttaacc tacaatgcac cgactatagt tttctatact aaccagaccc aaaccggatg
      241 tggagttggt tccgcccaag ccgggccatt ctattgctct gctgaccgca agatttatat
      301 tgatacttct ttctatcaag aattatctag caagtataag gctgctggtg actttgccat
      361 ggcctatgtc atagctcatg aagttggtca ccatgttcaa aatgaattag aagttcttgg
      421 ggcctaccat caaaaacggg ctcaactatc cgataaggaa ggcaatgcgc taaatgttcg
      481 cttagaattg caagctgact attatgcagg agcctgggcc aaatatgttg atggacaagg
      541 catcttggat gctggtgata ttgacgaagc catgaatgct gcccatgcag ttggtgacga
      601 tactcttcag caggaagcct acggccgtac tgttccagac agttttaccc acggcacttc
      661 tgagcaaagg aaaaaatggt ttaacctcgg ctatacctat ggtgaccttg cccacgccga
      721 tacattctcc gtagagaaac cgtaaacatc catagcctct cgtagcaata aagaaactct
      781 tgcaacatac acttttgcaa gagtttcttt ataatataca actgacagaa gccaatttac
      841 acatacggta ccacttttaa atatgaaaac tgaatcatgt aaacttactc atcaactcta
      901 attagagcgg tatcctagtt tcaaacaata tcacaattat tatttagcaa actttaatta
      961 tttccagtta tctctgctgc agactgcaca gttccgtttg gaataatcac tgttttcaac
     1021 aagttatttt cctcgtgaat acgtctgtat aagataacca tgtagactgg aaataagatg
     1081 agaaaactat atgttgcatg acacaataaa gctaaaccga ttaattctgg tataatattt
     1141 aggaaatagt taggatgctt tacaacacga aacaaccaat gatcaacaaa acgatgatct
     1201 tttaccaaca tgagcttaac tgtccaaata tcacctaaca atcgtgttac ccaaaataac
     1261 attcccattg aaaacagcaa gaatatcaaa cctaagctac taagcgtatc aaacttggtg
     1321 tgtcgcacta ctgcttcaac caaacatgat aaataaaata gaatatgtac aactgttaat
     1381 aatttagtat tttgaacacc aaattctgaa ccgccgtttt tcaaaatccg tttttcgttt
     1441 ttaattgaaa tcttcaaaaa atagattctc acccaaaaaa tacaaataac caaaaaaaca
     1501 atcataaatc cctcctgtat tttaacagat tttatcaaac acattcatta tacaaagtta
     1561 gactgtcgcc attctattcc tctcctgttt ttttcctatt ttccatttga gactacaata
     1621 aaagagacct gttttcaaaa atggatgaaa tcatgtctct tttatagtct ttt
//
LOCUS       DATUCG010000220         1671 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.223, whole genome shotgun sequence.
ACCESSION   DATUCG010000220 DATUCG010000000
VERSION     DATUCG010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.223"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(<1..1284)
                     /locus_tag="VCA20_002387"
     CDS             complement(<1..1284)
                     /locus_tag="VCA20_002387"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012235.1,HMM:NF012869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1836095.1"
                     /translation="MEKNVKLFYYLRKCKTELITYFFVSVIYALILAATGFVYARVSE
                     SALSGNQQVFLISSLIAVGFFICDIYFDYLPRYLKFKLVNRIMELTRNELVATYAVED
                     IAQFEEKASSDRVSVLVNNLDVLENGFLIPGLSMVTSVLVFTFSLIGALYLQGTMTII
                     MLVLCFIPFLAPLINNKILAHTTKESQEEKNVYLKRFSEFVQAFSFIRISNITPVFEK
                     NLETSSQEYAKRAILFSKKQSQTYAVSYGLSSIVYSGSWIIGGIFVFQGMLKISDLIA
                     MTTLMGTVAGPIQTMSGLITEYLASRTVVEELTTILKGQSHEDKVQQELTEPIRTLTL
                     DRVSSHNQQHPIFDNFSYQFLADKKYAIVGKSGSGKTTLLRLLLGVQSPNEGAIFINQ
                     INISEVPQASYFDKMYYLPQKTAILATTIGDNLTLF"
     gene            complement(1409..1549)
                     /locus_tag="VCA20_002388"
     CDS             complement(1409..1549)
                     /locus_tag="VCA20_002388"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1836096.1"
                     /translation="MKIISEASKEKIITMCGANQFSHHASAPKFNVELDKKSFSKEEK
                     IS"
BASE COUNT          579 a          306 c          251 g          535 t
ORIGIN      
        1 aaacagtgtc aagttatctc ctatagttgt tgctaaaata gctgtttttt gcggtagata
       61 atacatctta tcaaaatagg aagcctgggg cacttctgaa atattaatct gatttatgaa
      121 aatcgccccc tcatttggtg attgaacacc tagcaagagt ctcagcaatg tcgtcttccc
      181 actaccactt ttaccaacga tagcatattt tttatcagca agaaattgat aagaaaagtt
      241 gtcaaatata gggtgttgtt gattatggga actaacccta tctaaagtta aagtccttat
      301 aggttctgtc aactcttgtt ggactttgtc ttcatgtgat tgtcctttca gaatagtcgt
      361 caattcttcc acgaccgttc gactagcaag atactctgta atcagtcctg acatggtttg
      421 aatgggacca gcaaccgttc ccattagtgt cgtcatagca atcaagtctg aaattttcag
      481 catcccttga aaaacaaaga tacctccaat aatccatgaa ccagaataga caatactact
      541 cagaccgtaa gatactgcat aggtttgaga ttgctttttg gaaaatagga tggctctttt
      601 agcgtattct tgactagaag tctctaggtt cttttcaaag actggcgtaa tattactgat
      661 acgtatgaaa gaaaatgctt gtacaaactc actaaatcgt tttaaataga catttttctc
      721 ttcttgactt tctttagtcg tatgagccaa aattttatta tttatcaacg gtgctaagaa
      781 agggataaag caaagcacca acatgataat agtcattgtt ccttgcaaat aaagtgctcc
      841 aattaaggag aaagtaaata caaggacgct cgttaccatt gataaacctg gtattaaaaa
      901 gccattttct agtacatcta aattattcac taaaacactt accctatctg agcttgcttt
      961 ttcttcaaat tgagcaatat cctctactgc ataggttgca accaactcat ttcttgtcaa
     1021 ttccatgata cgattgacca atttaaactt gagatagcgt ggcaaataat caaaataaat
     1081 atcacaaatg aagaatccaa ctgctataag agaacttatc aaaaatacct gctgatttcc
     1141 agacaaagcc gactcagaaa cccgagcata gacgaaacct gtcgctgcta aaatcagagc
     1201 ataaatgaca gatacaaaaa aatatgtaat caattctgtc ttacattttc taaggtaata
     1261 aaacaatttt acatttttct ccataattct aaaagaaaag tgattgaaaa tattttaagt
     1321 tattttcaat caccaaatta agtataatat tttacaaaaa tcatgttata aaattcaatt
     1381 gatgatcaaa tttaaatata aagcgttatc aactaatctt ttcttccttg gaaaaagact
     1441 tcttatccaa ttctacattg aatttaggtg ctgatgcatg atgtgaaaat tgattagcac
     1501 cacacatagt gattattttc tcttttgaag cttcagatat tattttcata ctctattcct
     1561 cctacttaaa gaaaaacaat ggcacatgaa attgtaaaat atttaaaaac ccgtggtgct
     1621 agttcatttc aaactacaat aaaaagccca aatggactat actgtaagtg a
//
LOCUS       DATUCG010000221         1648 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.224, whole genome shotgun sequence.
ACCESSION   DATUCG010000221 DATUCG010000000
VERSION     DATUCG010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.224"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            <1..204
                     /locus_tag="VCA20_002389"
     CDS             <1..204
                     /locus_tag="VCA20_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEP1836097.1"
                     /translation="GKGVEKNRLGLIFEEFYRADDTRQQVEDHGLGLAIVKNIMERLD
                     GQVSVEATPGLRFIFRLPRKDMK"
     gene            201..875
                     /locus_tag="VCA20_002390"
     CDS             201..875
                     /locus_tag="VCA20_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEP1836098.1"
                     /translation="MTRILIAEDDVEIAYLERDYLEMQGYQVDLVHDGGLVEDQLKKE
                     DYALLMLDVMLPNKTGYDLCRELRDKVDFPILMVTSKQETMDVVRGLGLGLGADDYIS
                     KPFDPMELVARVKAHLARYQRFQKDNDQNELTVGNMTIFLDNWKVTLDGQEIKLPNRE
                     FELLVYLAKHPNQVFSKDHLFETILGYDYVGDSATVAVHVNRLRDKLGVDLIETVWGA
                     GYRLND"
     gene            987..1598
                     /locus_tag="VCA20_002391"
     CDS             987..1598
                     /locus_tag="VCA20_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1836099.1"
                     /translation="MENNNQQQEIIEVAENKKTGVWEAVKQKVTGMSKKSIILLSVGV
                     LALVTAGFVVAEVYENKLEALGDQIERQVGIADDDDNQQVIAQANASSASSVNTTSAS
                     QLATSYGVKLVDTSELDGTYTATSGNDTYTLIVKGNQATLTEVDKDGDQDIEQVIFDL
                     DKKLAYIDGESDSYTLNGPTLTLTEIDANDTTLEKLILNKNQK"
BASE COUNT          556 a          252 c          381 g          459 t
ORIGIN      
        1 ggaaaaggtg tagaaaagaa tagacttggc ttgatttttg aagaatttta cagggcagac
       61 gataccagac agcaagtaga agaccatggt ctggggttag cgattgtaaa aaatattatg
      121 gagagactgg acggccaggt atctgttgag gcaactccag gtcttcgatt tatctttaga
      181 ttacctagaa aggatatgaa atgacacgga ttttaatcgc agaagacgat gtagagattg
      241 cttatttgga gcgagattat ctggaaatgc agggctacca agtagatttg gtacatgacg
      301 gtggtcttgt agaggaccaa ctcaaaaaag aggattatgc tcttttaatg ctggatgtca
      361 tgctcccaaa taaaactggc tacgatcttt gtcgtgaatt acgggataag gtggacttcc
      421 ctattttgat ggttacatcc aagcaagaaa ctatggacgt ggtgagaggg ctaggcttag
      481 gcttgggagc ggatgattac atcagcaaac cttttgatcc catggagttg gtggcgcgtg
      541 taaaggctca tttggctcgc taccagcgtt ttcaaaagga taatgatcag aatgaattaa
      601 cagttgggaa tatgactatt ttcttggata attggaaagt gactttggat ggtcaggaaa
      661 tcaaattgcc caatcgtgaa tttgaattgc tggtttatct agcaaaacac cctaaccaag
      721 tattctctaa agatcacttg tttgaaacca ttttgggata tgactatgtt ggagatagtg
      781 cgacagttgc tgttcatgtc aatcggctcc gagataaatt gggagtggat ttgattgaga
      841 ctgtctgggg agcaggctat cgtcttaatg attaatttat aagtgtttag aatttgttta
      901 gaaagtttct ctaaagtgtt aaaacctatc ctctataata gacttatcag caagagctga
      961 agaaaacaag ttaaaaagag gataaaatgg aaaataacaa tcaacaacaa gaaattatag
     1021 aagtggcaga aaataagaaa acaggtgtct gggaagcagt caaacagaag gttacaggca
     1081 tgagtaagaa atcgattatc ttattgtcgg taggtgtctt ggcacttgtg acggctggtt
     1141 ttgtcgtagc agaagtctat gaaaataaac tagaggcatt aggagatcaa attgagcgac
     1201 aagttggaat tgcagatgat gatgacaatc aacaggtaat agcacaagct aatgcatcat
     1261 cagcctcatc agtgaataca acttctgcta gtcaactagc aacaagttac ggtgtaaagt
     1321 tagttgacac ctctgagcta gatggtactt atacagcaac atctggaaat gacacctaca
     1381 cgttgattgt aaaagggaat caagcaacct tgacagaagt tgacaaagac ggagatcaag
     1441 atattgaaca agttatcttt gatttagata aaaaacttgc ctatattgat ggcgaatcag
     1501 atagttatac cttgaacggt ccaactctta ctttgacaga aattgatgcc aatgatacaa
     1561 ccttagagaa actaatactc aataaaaatc aaaaatagca tattgaggtc aatttctatg
     1621 tgtaaaattt caatatctaa gttcagat
//
LOCUS       DATUCG010000222         1633 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.225, whole genome shotgun sequence.
ACCESSION   DATUCG010000222 DATUCG010000000
VERSION     DATUCG010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.225"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(48..704)
                     /locus_tag="VCA20_002392"
     CDS             complement(48..704)
                     /locus_tag="VCA20_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEP1836100.1"
                     /translation="MSVKDFMTRKVVYISPDTTIAHAADIMREQDLHRLPVIENDKLV
                     GLVTEGTIAEASPSKATSLSIYEMNYLLNKTKVKDVMIKNVITVSGYASLEDAAYLMY
                     KNKVGILPVVDNGQLYGVITDRDIFAAFLHVSGYGEEGVRARFLVENKAGELEKIIRL
                     VADKDYNIVSTVQLATKSGKVVIEVQIDGKVDVEEVRTLFEGAGIQVDSLVPTVAKNI
                     "
     gene            complement(788..1543)
                     /locus_tag="VCA20_002393"
     CDS             complement(788..1543)
                     /locus_tag="VCA20_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEP1836101.1"
                     /translation="MIKVEEILSTLSPNQKVNYDKVFQKMASTWQEARLRPNILMHVC
                     CAPCSTYTLEYLTQYADVTIYFANSNIHPKAEYQRRAAVTAQFVKEFNQKTGQSVGYL
                     EADYSPQEFFKKTQHLANEPEGGDRCKVCYDYRLDKTAEKAVELGFDYFGSALTISPH
                     KNAQTINAAGIEVQKLYDTQYLPSDFKKNGGYQRSVQMCEEYDIYRQCYCGCVYGANA
                     QGVNLVQVKKEALEFLKTHRDYSHIPFKVLDKN"
BASE COUNT          495 a          379 c          273 g          486 t
ORIGIN      
        1 ctatgctaga atgatagcta aagaaaaaaa ccgagaaatc tcggttttta aatattttta
       61 gcaacagtcg gaaccaagct atccacttgg atacctgcac cttcgaaaag agttcgaact
      121 tcttcaacat caacttttcc gtcaatttgc acttcgatga caacttttcc agacttggta
      181 gcaagttgca cagtagatac aatattataa tccttatctg ccaccaatcg aataattttt
      241 tctaactccc ctgccttatt ttcaacaagg aaccgagcgc gtactccttc ttctccataa
      301 cctgagacat gaagaaaagc tgcaaaaata tcacgatcag taatgacccc atatagttga
      361 ccgttgtcaa caactggaag aatccccact ttgtttttat acatgagata agcagcatct
      421 tcaagactag cataacctga gactgtgata acattcttaa tcatcacatc cttgacctta
      481 gtcttattca aaaggtagtt catctcgtaa atagataggc tagttgcttt cgatggacta
      541 gcttctgcga tcgttccctc agtgaccaaa ccaaccaact tatcattttc aataacaggt
      601 agacggtgca aatcctgttc acgcataata tctgcagcgt gtgcaatggt tgtatctggt
      661 gaaatgtaaa caactttacg ggtcataaaa tctttaacag acataggact tcctcctttg
      721 gtaaatcata ctttattata ccacaaatcc gaaaacgatt tcaatattta atgaaaagaa
      781 atgaaactca attcttatcc agcaccttga aaggaatgtg ggaataatct ctgtgcgttt
      841 ttaaaaattc caaggcttct ttttttacct gtacgagatt gacaccttgg gcattggctc
      901 catagacaca gccacaatag cactggcgat aaatgtcata ctcttcacac atttgaactg
      961 aacgctgata gcctccattt ttcttaaagt cactcggaag atactgggta tcgtataatt
     1021 tctgcacctc aatcccagca gcattgatgg tctgcgcatt cttatgtgga ctgattgtca
     1081 aagccgaacc aaaatagtca aaacctagct caactgcctt ctccgcagtc ttatccaacc
     1141 gataatcata gcaaacctta caccgatctc ctccctctgg ctcattggcg agatgctgag
     1201 ttttcttgaa aaattcctga gggctataat cagcttctag gtagcctacc gactgtccag
     1261 ttttctgatt aaattctttg acaaactggg ccgtcacagc tgcccgccgc tggtactccg
     1321 cctttggatg aatattggaa ttcgcaaaat agatggttac atctgcatat tgagtcaagt
     1381 actccaatgt ataggtcgag catggggcac aacagacatg catgagaatg ttcggtcgaa
     1441 gcctcgcttc ctgccacgtg cttgccattt tttgaaacac tttatcatag ttaacctttt
     1501 gattcggtga tagagtcgat aaaatctctt caactttaat catacaaacc tccaaatata
     1561 cattcattat actatatttg aagattactg catagatagc aataacatga gataacactc
     1621 cccatttcca cct
//
LOCUS       DATUCG010000223         1622 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.226, whole genome shotgun sequence.
ACCESSION   DATUCG010000223 DATUCG010000000
VERSION     DATUCG010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.226"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            144..812
                     /locus_tag="VCA20_002394"
     CDS             144..812
                     /locus_tag="VCA20_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SatD family protein"
                     /protein_id="HEP1836102.1"
                     /translation="MNYIAIIGDIVNSKQTSNRSSIQERLKQQLNRINHSFAQDFASP
                     FTITKGDEFQALCKPNPYIFLIIDQIQLAFRDEVEIRFGIGLGEILTAIDPKLSIGAD
                     GPAYWEARKAIDFIHDNHDYGTSKIAFSSEYKQIDRVINPLLTSSDFIKAAWNRSQTD
                     LFETLISKKIYQEDFTQKPIAEKMKLSQSAFTKRLKSSGIKLYLRNRQTAMNLILQLS
                     QKEG"
     gene            816..1556
                     /locus_tag="VCA20_002395"
     CDS             816..1556
                     /locus_tag="VCA20_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HEP1836103.1"
                     /translation="MSFSVISSYLIQHPVLTALLIAHFLSDFTFQSQAQANAKKLHLK
                     ALFMHLFIVAIPLLILALLTPVQNGDLFFQVWLSHLAIDYVKYFLNKHHWIKDSWEAG
                     AFLADQLLHIISIIILYHTIGVNVASRSLWIEPNYILLQILFILLISKPVNILFKLYF
                     SKYQVTEGEEEQTVTGAGALIGQLERFIMGIFLLLGQYIAIGLVFTAKSIARYDKISK
                     SQASAEYYLIGSLFSIISVLVLYVLLIL"
BASE COUNT          504 a          333 c          276 g          509 t
ORIGIN      
        1 atagaagtag caaagcagga aaaatgattt tcgaacttca acattttccg atataatgga
       61 aatattcaaa aatgtccttt ttaacggaca atctttatat tgtccgttat aatggaaata
      121 taatagaaat cagaggacaa ggtatgaatt atatcgcaat aataggagat attgtcaatt
      181 caaaacaaac ttccaaccgc tctagtattc aagagcggct aaaacagcag ctgaatagaa
      241 tcaatcattc cttcgctcag gacttcgcat cgccatttac cattacaaag ggagatgagt
      301 ttcaagcctt atgcaagcct aacccttata tttttttaat aattgaccaa atccaactag
      361 cctttcgtga tgaggtggag attagatttg gaattggttt gggtgagatt ctaacagcta
      421 tcgatcccaa gctaagtatt ggagcagatg gtccagctta ttgggaagca cgcaaggcta
      481 ttgacttcat tcatgacaac catgactatg gcaccagcaa aattgcattt tcatctgaat
      541 acaagcaaat cgatcgtgtg attaacccct tgctaacttc tagcgatttc atcaaagctg
      601 cctggaatcg ttcgcaaacg gatctgtttg agaccttaat ctccaaaaaa atctatcaag
      661 aagatttcac acaaaagcca attgctgaga aaatgaaact cagtcaaagc gcctttacca
      721 aacgtttgaa atccagcgga atcaaactct acctgcgtaa tcgtcaaact gccatgaatt
      781 tgattctcca gctcagtcag aaagaaggat aaagcatgtc attttcagtt atttctagct
      841 atcttattca acatcctgtt ttaaccgcct tgcttattgc ccacttttta agtgatttca
      901 ccttccaaag tcaagcacag gcgaatgcaa aaaaactgca tttgaaagct cttttcatgc
      961 accttttcat tgttgctatc cctcttctta ttctagcctt acttaccccc gttcaaaacg
     1021 gagatttatt ttttcaagtt tggctgagtc atcttgcgat tgactatgtc aaatattttc
     1081 ttaacaaaca ccattggata aaggactcct gggaagctgg agcattccta gctgatcaat
     1141 tgctacatat aatctcaatc atcattctct atcatactat cggtgttaat gtcgcttcac
     1201 gctcactctg gattgaacca aattatatcc tcctgcaaat cctttttatc cttctgatca
     1261 gcaagcccgt caatattctt tttaaactct actttagcaa atatcaggta acagaaggag
     1321 aagaagaaca gactgtgaca ggtgcaggag ccttgattgg tcaattggag cgcttcataa
     1381 tggggatttt tctgcttctg gggcaataca tagctatcgg attagtattt acagcaaagt
     1441 ccatcgcacg ctatgataaa atatccaaaa gccaagcctc tgctgaatac tatttaattg
     1501 gttcgctatt tagtatcatt agcgtcctag tattgtacgt tttattgatt ttataaaaga
     1561 aaaaagttta catattttat tttatgtaaa cttttgttat gtgactttta gtccgtcacg
     1621 cc
//
LOCUS       DATUCG010000224         1615 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.227, whole genome shotgun sequence.
ACCESSION   DATUCG010000224 DATUCG010000000
VERSION     DATUCG010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.227"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(104..1285)
                     /gene="icd"
                     /locus_tag="VCA20_002396"
     CDS             complement(104..1285)
                     /gene="icd"
                     /locus_tag="VCA20_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019804703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEP1836104.1"
                     /translation="MAEKILMREGRLIVPDYPVIPYIEGDGIGRDIWSVSQKVIDAAV
                     KKAYAGQRSIQWREVFAGEKAYELTGTSLPNETLEIIREHLVAIKGPLGTPIGKGHRS
                     LNVALRQELDLYACVRPIRYFKGVPSPLKEPEKTDITIFRENTEDIYAGIEWEAGSDE
                     VAKVISFLQEEMGVVNIRFPESSSIGIKPISEEGSKRLIRSAIEYALEHQLKKVTLVH
                     KGNIQKFTEGGFRKWGYELAKEEFATELAEGTLVVNDIIADNFLQQILLAPENFQVVA
                     LTNLNGDYASDALAAQVGGIGISPGANINYQTGHAIFEATHGTAPDIAGRDLANPCSL
                     LLSACMMLEYIGWKEAAALIIQALEGAFINKNVTLDFAKILDVAHQSTSRFSEVLIDS
                     I"
     gene            complement(1288..>1615)
                     /locus_tag="VCA20_002397"
     CDS             complement(1288..>1615)
                     /locus_tag="VCA20_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790817.1"
                     /note="catalyzes the formation of citrate from acetyl-CoA
                     and oxaloacetate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEP1836105.1"
                     /translation="DPRQEYLKEMARDLTFGTDDEAFYRLSEEVESFIKNKKGLIPNV
                     DFYSATVYHVLGIDSDIFTLIFAMSRMAGWIAHVQEQRKNNKLIRPRSSYLGGRNLVY
                     IPIGKR"
BASE COUNT          478 a          390 c          259 g          488 t
ORIGIN      
        1 aaaaggtatg actcccctag aatttaggga agcatacctt ctaaaaaact aactttttta
       61 actgtccatt ttatctggtg cttgacattt tactttttct tcattaaata gaatctatta
      121 gaacttctga aaatcgacta gtagattgat gagcaacgtc caaaatttta gcaaaatcaa
      181 gcgtaacatt tttatttata aaagcgcctt ccaaggcctg aataattaaa gcagctgctt
      241 ctttccagcc tatatattcc aacatcatac aagccgaaag caagagggaa catggattag
      301 ccaaatccct tcctgcaata tcgggagcag ttccatgtgt cgcttcaaag atagcatgac
      361 ctgtctgata attaatgtta gcacccggtg agatgcctat tcctccgacc tgagccgcaa
      421 gagcatctga tgcataatct ccatttagat ttgtaagagc aaccacttgg aagttttccg
      481 gcgccaataa aatctgctgc agaaagttat ccgcaataat gtcatttact accaaagtcc
      541 cctctgcaag ttctgtagca aattcttctt tcgccaattc gtaaccccac ttgcggaagc
      601 caccttctgt aaacttttga atatttccct tatgaactaa ggtcaccttc ttcaattgat
      661 gttctagggc atattctatt gcagagcgaa tgaggcgttt actcccttct tccgaaatcg
      721 gtttaatacc aatacttgaa gattctggaa aacggatatt taccactccc atttcttctt
      781 gcagaaagga aatgactttg gcaacttcat ctgaaccagc ctcccattca atacctgcat
      841 aaatatcttc tgtattttca cgaaaaatag taatatccgt cttttcaggc tctttcagcg
      901 gacttggcac tcccttaaaa tagcgaatcg gacgcacaca ggcatacaaa tctaattctt
      961 gacgaagtgc aacattaaga gaacgatgac ctttcccaat cggtgttccc aagggacctt
     1021 tgattgcaac taaatgctca cggattattt ccagtgtctc attcggcaaa gaagttcccg
     1081 ttaattcata agccttttct cctgcaaaaa cctccctcca ctgaatagaa cgttgacctg
     1141 cataggcttt tttcactgca gcatcaataa ctttttgact gactgaccaa atatccctgc
     1201 caataccatc tccttcaata tagggaataa cgggataatc tgggacaatt aaccgtccct
     1261 ctctcatcag gattttttct gccatattta cctctttcca atcgggatgt aaaccaaatt
     1321 cctaccacct aagtaggaag agcgcggacg aatcaattta ttatttttcc gttgttcctg
     1381 gacatgtgca atccaccccg ccatacggct cattgcaaaa atcaatgtaa aaatatcact
     1441 atcaatacct agcacatggt aaactgtcgc cgaatagaag tctacgtttg gaattaaacc
     1501 ttttttgttt ttaataaagc tctctacttc ctctgacaat ctataaaacg cttcatcgtc
     1561 tgttccgaat gtcaaatcac gcgccatctc cttcaaatac tcttgacgag gatca
//
LOCUS       DATUCG010000225         1585 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.228, whole genome shotgun sequence.
ACCESSION   DATUCG010000225 DATUCG010000000
VERSION     DATUCG010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.228"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            389..760
                     /locus_tag="VCA20_002398"
     CDS             389..760
                     /locus_tag="VCA20_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1836106.1"
                     /translation="MKKFVYAFLVLAYVAIAYLSVYILPHYYVGAIMGAAGASLGARF
                     KQFKEVKAFPDKALLAYFKRDKKKASSLLLVLLGIFLFVSLVLEFNWQYGLAFVVAIS
                     YAMLWNVLHIQFLRKYYFKNA"
     gene            complement(782..1489)
                     /locus_tag="VCA20_002399"
                     /pseudo
     CDS             complement(782..1489)
                     /locus_tag="VCA20_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942636.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
BASE COUNT          455 a          308 c          360 g          462 t
ORIGIN      
        1 acttgacaaa gggctttaca tatcagtcat ttgaattagc tttgatacat cgtcactttc
       61 ggcttgccgt actctacgaa agtaacttgt ttcgcaagtc ttatttccaa cctagaacag
      121 cctagaggct gttctagctg cctgcacctc agttccttgt ctgaaagctc ttcatttgac
      181 tatagcaaat ccttgaaaaa atctctggct caggctaaaa aatgctaccc aacagcccgc
      241 tctaggctga cgagcaggat tattatagca agcttttgac agaagttgag ttagaaagtc
      301 aattgtcata gtagattatt ttgacttgta tttgcgataa aatttatgtt aaaataggta
      361 cggaaacaaa tagataggga ggtttgtcat gaaaaaattt gtatatgcct tcttggtgct
      421 tgcctatgtt gcaattgctt atttgtcagt ctatatactt cctcattatt atgtaggtgc
      481 gataatggga gcggcaggag caagtcttgg tgctcgtttc aaacagttca aggaagtgaa
      541 agcttttcct gataaagcct tattggctta ttttaagagg gataagaaaa aggcaagtag
      601 tttattattg gtccttctgg gaatattcct ttttgtcagc cttgtactgg aatttaattg
      661 gcaatatggc ttagcttttg tagtggcgat cagctatgct atgctttgga atgtccttca
      721 tatccaattc ctacgaaaat actattttaa aaacgcatga gagtttcatg cgttttcttt
      781 tctaaatact ttgtccagct tttaattttt tcataaagat gatggaactt ggaatcagga
      841 cgatgatgct tccgatccag gcggcggggt tagcggcggc ggttccataa aaaccgaaat
      901 actgcatgcc gatgatggcg actgctgctc ggaaaatcaa ttctccaaag cctgctagtg
      961 ttgggataaa gcccttgcca agtccctgaa taaagctacg aagaataaag agggaagcaa
     1021 caatccagta acaagagccg ttaatgatgt agtaaacaag agccaagtcc agcactgttt
     1081 gactggcatt tggcagaaag agaccagaga agaagcgatt gcccaagatg agaatgaccg
     1141 caaatataat ggcccaagca atgctgagga gtaatgagtg gcgcactcct tctcgaatac
     1201 ggtcatagag cttggcaccg tagttctgag cagtaaaggt tgcgatagcc aagcccagat
     1261 tgaccatggg cagcacagcc aactggtcag tacgaagggc aatggcctgg gcggcgatgg
     1321 cgtctgttcc tagttggtta accataaact gcaaggtcat ggctccgatg gcgataatac
     1381 tggcttgaaa tcccattgga aaagcaagct gggcatgttt cttgagatta cctctatcca
     1441 attttaaatc agctagtccg atatgatagt gaggtacctt gtggataata gacttgttcg
     1501 gtaactatat catctcatgg ttgatgatgc cacaaatcaa ctcagctctt aattcaaacc
     1561 gttttctacg attacgatag ggctg
//
LOCUS       DATUCG010000226         1576 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.229, whole genome shotgun sequence.
ACCESSION   DATUCG010000226 DATUCG010000000
VERSION     DATUCG010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.229"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            265..1182
                     /locus_tag="VCA20_002400"
     CDS             265..1182
                     /locus_tag="VCA20_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017769516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEP1836107.1"
                     /translation="METLQKINFLEAIDSYESLTKAAKHLFISQPYLSKVIKQMEDQL
                     DTALIYNHHTGSQLTYAGKRYLYYLKEIYRLENKIKDEMYLIRNQQQGEIFLGINPGL
                     ASFFLAEFIPQFQSIHPKIFLKLIENNQNITEKMILLDQIDLAIGMSPLLYQDELSTI
                     TIYKEKIYLLVPQQSSLFSTQQAKKINTFHYSYNKLESEPLIITPLEYGLGSLIRSFY
                     NKHHININPILTTSTVPTAINLSLAGMGSTYIPESLIETLPSDSSYNLYKLEDSSLFA
                     EYILIYNKNKSLSLLTLKLLHTIQEKIAK"
     gene            complement(1361..>1576)
                     /locus_tag="VCA20_002401"
     CDS             complement(1361..>1576)
                     /locus_tag="VCA20_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEP1836108.1"
                     /translation="HLWTPLRQNMAGAEEHNDWKVMALRRTIETRFSELCRLFDIEHT
                     LTRGLARLQLRMEQIILAHNLRYFEMN"
BASE COUNT          553 a          303 c          203 g          517 t
ORIGIN      
        1 tcgtttcata atcttcaaag attaaaaaaa aaaagaacat ataactaaaa agttatttag
       61 tcttatcatg tctatgtaaa gtctgcctag aactccggct tattttacca gctatgctat
      121 aataattaaa aatgtatttt caacctttaa acgtcacacc tatcaccaat catcataact
      181 gattggtaat agataactag aatccaacta gcgtaacaat aaacaggcta cattttcata
      241 aatatagaaa gtcaggtatt tcccatggaa acattacaaa aaattaactt cttagaagct
      301 atcgattctt atgagtcact aacgaaagca gctaaacatt tatttatttc acagccctat
      361 ctcagcaaag ttattaaaca aatggaggac cagctagaca ctgcacttat ctacaatcac
      421 catactggta gccaactaac ttatgcaggt aaacggtatc tttactattt aaaagagatt
      481 taccgtcttg aaaataaaat aaaagatgag atgtatctga tacgcaatca acaacaagga
      541 gagatttttc tgggaattaa ccccggatta gcttcgtttt tcttagccga atttattcct
      601 caattccagt ccattcatcc taaaatcttc ttaaaattga ttgaaaataa ccagaatatt
      661 actgaaaaga tgattttgct tgatcaaatc gatttagcaa ttgggatgtc tcctcttctc
      721 taccaagatg agttgtcaac cattacaata tataaggaaa aaatttatct attagttccc
      781 caacaaagta gtcttttttc tactcaacaa gcaaaaaaaa ttaacacttt tcactacagc
      841 tataataaac tggaatctga acctctcatt attacaccat tggaatatgg gttagggagc
      901 cttattcgtt ctttctataa caaacaccat atcaatataa accctattct aacaacaagt
      961 acagtcccaa ctgctatcaa tctatctcta gcaggaatgg gttctactta tattcctgaa
     1021 tcactaatcg aaacgcttcc ttcagatagt tcttataatc tatataaatt agaagattca
     1081 tctttatttg ccgaatatat tttaatatac aacaaaaata aaagtctgtc tcttctcaca
     1141 ttaaaattgt tacatactat tcaagagaag atagcaaagt aggtaatctt agtacgaaac
     1201 cagtagcaag taatccaata tttttaactc taaaacaaat catctatttc tacgttttca
     1261 ttatttttat tattattagc attcaattct tttaccaata tctctaaccc tctagctaat
     1321 tttttgtact tatctagata gtatccaaat acccgtggtg ctagttcatt tcaaaatagc
     1381 gtaagttatg agctagaata atttgttcca ttcgtaactg tagtctagct agacctctag
     1441 ttaatgtatg ttcgatgtca aagagacgac acagttcaga aaaacgtgtt tcaatagttc
     1501 gtctcaaggc catcactttc caatcgttat gttcttcagc ccctgccata ttttgtcgaa
     1561 gtggcgtcca tagatg
//
LOCUS       DATUCG010000227         1543 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.230, whole genome shotgun sequence.
ACCESSION   DATUCG010000227 DATUCG010000000
VERSION     DATUCG010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.230"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            81..1070
                     /locus_tag="VCA20_002402"
     CDS             81..1070
                     /locus_tag="VCA20_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018379641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1836109.1"
                     /translation="MNIWIVDKILSKKYLWGVIFTCWIFFGIILSIFDSSLKNNYVYV
                     LGVNLLLFAQIVTYHCLMRKEGLLSLSISGITIDGYSLFSICNRFGTYLQKWYSIIIS
                     IAIGIIYIISLILLKVLMWNQVITVYGSITLIFTVFIAIQLYLKYIFYILTLRKIANL
                     EFTTFTPISLHNPSEATWIVKFTDTMSTFGNYFGVLGIAYTLLFYLTTPLSAISFSNN
                     KLNINTSNDIVFIITWGIIAILIGLGYILFDYLWKTYIKIIIKKIKRNRLLEYDNSPN
                     QQSQLNKDFIDLFNLYRDTPNFPELFNGQYVNPLGYIPVIINLYNVIIPFLSQ"
BASE COUNT          526 a          236 c          203 g          578 t
ORIGIN      
        1 aatttatcac acaaaaaata tgaataacta caaattatgg tatactagtg ataataaatt
       61 gttatcggta ggagaatgct atgaatatat ggattgttga taaaattctt tcaaaaaaat
      121 atctatgggg tgttattttt acatgctgga ttttttttgg aataattcta tcaatttttg
      181 actcttcgct gaagaataac tatgtatacg tgctcggtgt aaatcttttg ctatttgcac
      241 agattgtaac gtatcattgc ctaatgagga aagagggtct actttcattg tctatttcag
      301 gcattacaat tgatggatat tcgctatttt cgatatgtaa cagatttgga acttatctac
      361 aaaaatggta cagtatcatt atttctatag caattggaat tatctatatc atttcattga
      421 ttttgctaaa agtattaatg tggaaccaag ttattacagt atacggtagt attactttaa
      481 tctttacagt ttttattgca atacaactat acttaaagta catattttac attcttactt
      541 taagaaaaat tgcaaatcta gaatttacta catttactcc aatttctctt cataatccat
      601 ctgaggctac ttggattgtt aaattcacag acacaatgag cacttttggt aattactttg
      661 gtgtccttgg gattgcttat actttattat tttatttaac aactccttta tctgctatat
      721 cattttcaaa taataaacta aatataaata cgtctaatga tattgtattc ataataacat
      781 ggggaattat cgctatatta attggtttgg gttatatatt gtttgattat ctatggaaaa
      841 cttatatcaa gattattata aaaaaaataa agagaaatag acttttagaa tatgacaata
      901 gcccgaatca acaatcacaa ttaaataaag actttatcga tctctttaat ttgtatagag
      961 acacacctaa tttccctgaa ttattcaacg ggcaatacgt aaatcctctt ggttatattc
     1021 cagtgataat caacctgtat aacgtaataa tcccttttct ttcacaataa taactgaaca
     1081 aaaagaagct gggtaagaca tccatgatgg ctccgccctc actcacacaa taatgaaaac
     1141 aaatataaag ccctcactag gtgtataatc ttaatgaggg agtttttgca ttctttttag
     1201 ttttcgtata aaaactaaaa aattccccct cctataaaaa ttttatcaat caaaaacagc
     1261 ttagaatcaa cgtttctgac atcgcgattc taagctgttt tctattttta agacttttta
     1321 cctagcctct ttttatttga tacttgccct tgttttagct aagtttatag catagtcaat
     1381 aaagaatgat aagcgttgca cattcatagt ttctcctaaa atgtttaaat tattctgtat
     1441 atctaattca atacaataaa tttctttttt tattaaatcc aatatgttat atttattcaa
     1501 caaattatcc agaatagagt actttccgcg atcaagttcg gat
//
LOCUS       DATUCG010000228         1512 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.231, whole genome shotgun sequence.
ACCESSION   DATUCG010000228 DATUCG010000000
VERSION     DATUCG010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.231"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            60..302
                     /locus_tag="VCA20_002403"
     CDS             60..302
                     /locus_tag="VCA20_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1836110.1"
                     /translation="MTPISIKKFVELTINNNPDINPKELEENLRAVLEEKEGGARCMN
                     CGAPIWVAGSALVGSHMCFTCLTGEADDSDDFEVLG"
     gene            639..1001
                     /locus_tag="VCA20_002404"
     CDS             639..1001
                     /locus_tag="VCA20_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013851909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1836111.1"
                     /translation="MTDHYRIIRKEINLTPGELEQIQELMKQEHADQFSPFVRQKLMD
                     LVERKQAVTDWFAFWQSQKIEQISRDILQVTIMAEQTQHVTAEHLRIILTCVQELMAE
                     VEKFIPLSTDFRDKYMGG"
     gene            1004..1348
                     /gene="mobC"
                     /locus_tag="VCA20_002405"
                     /pseudo
     CDS             1004..1348
                     /gene="mobC"
                     /locus_tag="VCA20_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
BASE COUNT          461 a          304 c          357 g          390 t
ORIGIN      
        1 cagtgataat tgtttagatg aataggtact agaaatagaa tcaggacgga ggtaaagcat
       61 tgactcctat ttcaatcaaa aaatttgtcg aattgactat caataataat ccagacatca
      121 atcccaaaga gcttgaagaa aacctgcgtg cagtacttga agaaaaagaa ggaggggcaa
      181 ggtgtatgaa ttgtggagct cctatttggg tagcagggag tgcactggta gggagccaca
      241 tgtgtttcac gtgtctaaca ggtgaagctg atgatagtga tgattttgaa gttttaggtt
      301 aagctatttc agaaattttt tgagaacccg atggctcttt ttcttttcct gtgataattt
      361 ttccagctct gtcttgttac actctatcta agtaaaaaaa tcaacccttg atttttgatt
      421 gggggtttgg gggcgaagcc accatagttt cttatcgtcg gctgacaaac ctgtaaggtt
      481 ttggttcagc tggcgattag attttgggtt attgtggaca caataactga gctcgcaaaa
      541 gccgaaaaag agccttttaa ggcttttagg aagcacccgt atcccatacg gtatcggggt
      601 tgacccgata gaggcaagta gagaaggagt ataacgagat gacagaccac tatcgtatta
      661 tccgtaagga aatcaatctg acaccaggag aactggagca gattcaggag ctgatgaagc
      721 aggaacacgc tgaccagttt tccccctttg ttcgtcaaaa actgatggac ttagttgaga
      781 ggaagcaggc agtaacggat tggtttgcct tctggcagtc ccagaagatt gaacaaatca
      841 gtcgagacat tttacaagtg acgatcatgg ccgaacagac ccagcatgtc acggcagagc
      901 acttgcgaat tatcctaact tgtgtgcagg agctgatggc agaagtagag aagtttattc
      961 cactcagtac agattttcgt gacaagtaca tgggaggtta gatatggaaa atctctatcg
     1021 taccaacctc aaaaaagtct ttctgacgaa ccaggagttg gcaactttga acgatcgtat
     1081 cggacagagt ggctgtaaaa atttctctgc ttatgccaga aatgttctgc tcaatcccaa
     1141 catgaccttc ttgaccattg ataccagtaa ctatgaggaa ctggtctttg aactgaaacg
     1201 tatcggaaac aacctcaatc aaatcgcacg gaccgtcaac cagactcaca tcttaacacc
     1261 aagcgaccgt gaaattttgg tgcaaggcat tggccaactc ataaaggaag tggagaagga
     1321 ctttaacatc aagtgccagc agctgaagct ggtctctgat tttggcatga gcaactacct
     1381 agactttccc ggctatggag agctggtgga gatgtatcag gccaacttga ccaacaatga
     1441 tcggctctat gattccagaa acgaccgcca gcaaataaag caggccaaaa tccatgccca
     1501 ccacttgatt ca
//
LOCUS       DATUCG010000229         1503 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.232, whole genome shotgun sequence.
ACCESSION   DATUCG010000229 DATUCG010000000
VERSION     DATUCG010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.232"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(666..1274)
                     /locus_tag="VCA20_002406"
     CDS             complement(666..1274)
                     /locus_tag="VCA20_002406"
                     /inference="COORDINATES: protein motif:HMM:NF014717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEP1836112.1"
                     /translation="MLKLIYYQYIFRKSEWLGALPVLIVAGMITGTCFTILINILANT
                     EVFIQDIPTPLFIAPIFFGGLTLFFLISTLIRLLLNIFKSEYRIWDIIGASRWQLSIL
                     IGGQFAIVTLISSFIGSILSIFPAKSYYILMQGRVGEEYFPSIPIRFSFMSVIFTTII
                     LTLVSYISSFYNAYLLLDGNFFTEDDRIQKKILKLEKLSYSG"
BASE COUNT          594 a          248 c          181 g          480 t
ORIGIN      
        1 taaagaaatt attaaagctg gaaataaaaa atatttagta tcaaaaatca acaaatcagt
       61 ataattaaca atattgatta ctaatagcga tatgattccg tttaagaaca tagatattat
      121 caatacaatt gttgaatata taatagattc caataatttg atttttatca tctggatatt
      181 tgatacccct attgtctcaa gtttcgaatt tatagacttg tcttgctcgg atataataat
      241 tgttacagaa ataagattag aaactataat gaacagcggg gcagctaaat agaataaaaa
      301 tgatatatat atttctttat tttgctcaac tccactcctt atcccattgt tagaaattag
      361 taacaatcca gtaattaatg taatagccgt taccatagat atagtaactg aacttaaaaa
      421 atatttcttt tcaattacat tccacctaga aataattgtt gcaaaattcc tccctttatt
      481 aaataaaatt cggagaattt ttaattcaat ccaaggtgat tgatacttta caaaaaacat
      541 atgaataact aatagataga agatagcaga agacatgaac gataatttat atttaacccc
      601 gcctggtaaa tcaagatagg gcactattat tgatccgatg atcaaggaaa gccataagaa
      661 caaatttatc ccgaataaga caatttttct aattttagaa tttttttctg tattctatca
      721 tcctcagtaa aaaagtttcc atctaacaat aaatacgcat tgtaaaatga gctaatataa
      781 ctcactaacg tcaatattat tgttgtaaaa ataactgaca taaaactgaa acgaattgga
      841 atcgagggaa aatattcttc tccaactcgt ccttgcatta aaatataata acttttcgcc
      901 ggaaagattg ataatatcga gccaataaaa cttgaaatca aagtaactat cgcaaactgt
      961 cccccaatta atattgataa ctgccaccta cttgctccta taatatccca aatcctatac
     1021 tcacttttaa agatattaag taaaagtcta attagagttg aaataagaaa aaaaagagtt
     1081 aatccaccaa aaaatatcgg ggcaataaat aaaggtgtcg gtatatcttg aatgaagacc
     1141 tcagtatttg ctagtatatt aattagaatt gtaaaacaag ttcctgtaat cattcccgct
     1201 acaattaata ctggaagagc tcctagccat tcactttttc taaaaatata ttgataataa
     1261 attagcttta gcatataatc tcttttcttc aaatcaaaat gaaaaacctt ataatatctg
     1321 attggtatag gcaattccaa aacaatagat actataaggc tcataaaaat taaaatatac
     1381 aaactagcaa ccaaacgcac taatcatact ccagttacca caccaactaa gactagttgt
     1441 gctaaaccgc ataccatcaa cttcatctgt ttcttcttcc acagataatg tttgtaattt
     1501 taa
//
LOCUS       DATUCG010000230         1495 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.233, whole genome shotgun sequence.
ACCESSION   DATUCG010000230 DATUCG010000000
VERSION     DATUCG010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1495
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.233"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(<1..324)
                     /locus_tag="VCA20_002407"
     CDS             complement(<1..324)
                     /locus_tag="VCA20_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor regulator N-terminal
                     domain-containing protein"
                     /protein_id="HEP1836113.1"
                     /translation="MTMKTFEEVTKKSKRKNLWKTVGLSSLIGFILLLIGIQTVKIIV
                     GAREGREVWEQTMLMEIAYPNINHDGIKIQSTSYVSGNLYFDLYKDLDGVPMAFGQHE
                     IYYDLF"
     gene            complement(305..808)
                     /locus_tag="VCA20_002408"
     CDS             complement(305..808)
                     /locus_tag="VCA20_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEP1836114.1"
                     /translation="MSIKLDQYEKEIIGIAEEISYYLQKSGAGPSDSQDIAQDLLVKL
                     LESDIVLPFEKLRAWLYRSAVRAYIDKYRRDKRYHEILQKEFFRREDLLVYDQEDYEE
                     LYQAVADLPEKYQQVIDLYYFQDMSVKEVAHILGTGQSWVKIILYRGRKQLRKVLEER
                     GYDYEDF"
     gene            complement(920..>1495)
                     /locus_tag="VCA20_002409"
     CDS             complement(920..>1495)
                     /locus_tag="VCA20_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEP1836115.1"
                     /translation="RLSCFIGVKTLTALSIVTEIGGLRAIPWVFSWSQNRVMLPGWYG
                     VGSSFKRFIDKHPDNLSKLQKMYESWPFFRSLLSNVDMVLSKSNLNIAFEYAKMCESE
                     EGRNIFHVILDEWQLTKEIILSIEQNDELLAELPFLKASLDYRMPYFNVLNYIQIELI
                     HRLRRGELSKEQENLIHITINGVATGLRNSG"
BASE COUNT          446 a          314 c          262 g          473 t
ORIGIN      
        1 aaacaggtca tagtaaattt catgttgccc aaaagccatt ggaacaccgt ctaaatcctt
       61 gtacaaatcg aagtagaggt tcccagatac ataactcgta ctttggattt taatgccatc
      121 atgatttata tttggatagg cgatttccat taacatcgtt tgttcccaca cctcacgacc
      181 ttctcgtgca ccgacaatga tttttacggt ttgaatgcca atcaacaata agataaagcc
      241 gatgagcgat gatagcccaa ctgtcttcca taaatttttc cgcttactct tttttgtcac
      301 ttcttcaaaa gtcttcatag tcatatcctc tctcttctag tactttcctt aactgcttgc
      361 gtccgcggta aaggataatt ttcacccaac tttgaccagt ccctaagata tgggcaactt
      421 ctttgacact catatcttga aaataataga ggtcaatcac ttgctggtat ttttctggca
      481 ggtctgcaac agcttgatag agttcttcat aatcttcttg gtcatagacc aaaaggtctt
      541 ctcttctaaa aaactccttt tgtaaaattt catggtagcg cttgtctcgc ctgtatttgt
      601 caatgtaagc ccgaactgca gaacggtaaa gccaagctct tagtttttcg aaaggaagga
      661 caatatcaga ttcaagcaat ttgactagaa gatcttgggc aatatcctga ctgtcagatg
      721 ggccggctcc tgatttttgc aaatagtagg aaatctcctc tgcaatacca ataatttctt
      781 tttcatattg atctagtttg atacttatcc cctcctttca ctgataaaac gaataagggg
      841 tgatttggtt acgttttctt attattatat atttttagaa tagaaaaaaa caacctccga
      901 agaagttgtt ttatgcctat taacctgagt tgcgcaaacc tgtcgccaca ccattgatgg
      961 tgatatgaat gagattctct tgttctttgc tcaattctcc acgacgaaga cgatgaatta
     1021 attcaatctg aatatagttg agcacattga aataaggcat gcgataatca agactggctt
     1081 ttaggaacgg tagttctgct aaaagctcat cattttgctc gattgacaaa ataatttctt
     1141 tggtcaactg ccattcatct aaaatgacat ggaagatatt tcgtccctct tcgctttcac
     1201 acattttagc atattcaaat gcgatattca aattggattt agacaatacc atatccacgt
     1261 ttgatagaag tgaacggaag aatggccagg attcgtacat tttttgtaac ttagacaaat
     1321 tatcagggtg cttatcaata aagcgtttga aacttgaacc aactccatac caccctggca
     1381 acataacacg gttttgtgac caggagaata cccatggaat cgcacgcaaa ccaccgattt
     1441 ccgtcacaat ggaaagagcg gttagtgttt tgacgccaat aaagcaagaa agccg
//
LOCUS       DATUCG010000231         1476 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.234, whole genome shotgun sequence.
ACCESSION   DATUCG010000231 DATUCG010000000
VERSION     DATUCG010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.234"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            248..1105
                     /locus_tag="VCA20_002410"
                     /pseudo
     CDS             248..1105
                     /locus_tag="VCA20_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735858.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            complement(1247..>1476)
                     /locus_tag="VCA20_002411"
     CDS             complement(1247..>1476)
                     /locus_tag="VCA20_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HEP1836116.1"
                     /translation="QEGYHLWTPFRKNMTGAEEHNNWKVMAMRRTIETRFSELCRLFD
                     IEHTLARSLAGLQLRMEQIILAHNLRYFEMN"
BASE COUNT          507 a          233 c          291 g          445 t
ORIGIN      
        1 catgatggat tattcatggc tagaaaatta taaaataagt cgattgagga aaccagtgga
       61 gaccgtattt tctagtttag agcagtttgg aatggagggg ttacgttgcc gaaacattca
      121 ggcgctcaaa tttaagacag aggcaatatt acttatctat tctcttttgc ttaaaagcag
      181 tcatactgaa tttggcatca gtttaaaata ttcccttgct tacgcttgat taactagcac
      241 aacgggtaag gaatttgctt ggaaattagt agactataaa gataatagag cagagctggt
      301 tgagaaagta aaagctatct ataccaaaac caagatacat atgcctacgt tagaaaagga
      361 caatcaactg atagatattg atccttttac gatatttgga ctcttcaata agaaaataac
      421 agaagagaat cgcatcaaaa ttctgacagc tatagcagaa ttattcaacc tccaaacgga
      481 aatcccatcc tcttttgaaa gtatatcagt actgatgaat cagaatgcga ctttctatta
      541 ttttatcgga gatcgtgatg agaaagatat tgatgactta tgggaacttt tcattgctgc
      601 tttgacatat agcaaggagc ctacggctga gaagaaagaa aaattttcac actattttga
      661 tttggctata aacaagaaag gaaatggcaa cagcaagatt acgatggcct tgtattggat
      721 tgctcccgac tttttcttga acttagatag cagaaataag tggtatgtgt acgaatctgg
      781 agagattcct actgatgtca tcaaaaaact accaaagatg aagccaaaaa tttctgccga
      841 tgattatttt tatatatcgg actctatgca tcattattta aacagtgcag agagcaaact
      901 gaaagatttt aaagaattat catccatggc ttggaaatac tctaaacaga tcaatcaaga
      961 agagaaggaa agaagtgctg gagcaatttc tccactggct gaggaagctg atactggaaa
     1021 cgagataggt agagaagagg tagtagaaga tagctatacg aaaaatcact tcctatcaga
     1081 agtctatatg acagaagaag agtatacaga tatgcatata attagtggat taacatgtct
     1141 gttcgtgata tactttgcat gagattgtgt attatcatac ttgtttctta aaaattagta
     1201 attagattat gaaatatgca atcaataatt ttaaataccc gtggtgctag ttcatttcaa
     1261 aatagcgtaa gttatgagct agaataattt gttccattcg taactgtagt ccggctaggc
     1321 ttctagctaa tgtatgttcg atgtcaaaga gacgacacag ttcagaaaaa cgagtttcaa
     1381 tagttcgtct catggccatt actttccaat tgttatgttc ttcagcccct gtcatgtttt
     1441 tacgaaatgg cgtccataga tggtaacctt cttgtt
//
LOCUS       DATUCG010000232         1375 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.235, whole genome shotgun sequence.
ACCESSION   DATUCG010000232 DATUCG010000000
VERSION     DATUCG010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1375
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.235"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(74..1351)
                     /locus_tag="VCA20_002412"
     CDS             complement(74..1351)
                     /locus_tag="VCA20_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEP1836117.1"
                     /translation="MNKQTYKEILTIAFPAMGENLLQMLMGVVDSYLVASIGIVALSG
                     VSLANNILAVYQAVFIALAAAVSSRLAQALGEGGSEGIGGTASESVKFTVFIGLLLGI
                     FSVLAGPIVLTSLGAESEVVQAGGLYLILVGGGAPFLGLMTSLSAILRTLGEPRFPMY
                     ISLLSNVLNALFSAFAVFVLHAGVAGVAIGTVLSRLIGCSLLWSRLPISLKPWTWSFD
                     KELLRLALPATGERLMMRVGDVVVVALITSLGTATVAGNAIGETLTQFNYMPALGIAT
                     ATIILTAKHRQDKAMVRHIFRTSFDLSLLFMFLVAGVTYFAGPFLISLYIKDIQAVQA
                     SQTVLFYAMLGVPFTAGTLIMTALWQGLGNARLPFYATTIGMWLVRIGIAYLLVTFLK
                     IGLSAIWIATILDNAFRAGVLFFQYRYSKQDSI"
BASE COUNT          453 a          333 c          248 g          341 t
ORIGIN      
        1 gtcctgctct tgtttcagtc gactatatat agctacaaac ttaaacgaaa ctgaaagttt
       61 tcttagcctt atcttaaatt gaatcttgtt ttgaatacct atactgaaaa aatagcaccc
      121 ctgctcggaa ggcattatct aaaatcgttg caatccaaat ggctgacaaa ccgattttta
      181 aaaatgttac aagtaaataa gctatgccaa tccgcactag ccacataccg atagttgtgg
      241 cataaaaggg taatctagca tttcccaaac cttgccaaag agctgtcata ataagagtgc
      301 cagctgtaaa gggaactccc aacatggcat aaaataatac tgtttgacta gcttgtaccg
      361 cttgaatatc cttaatataa agactgatta ggaaaggccc cgcaaaataa gtcaccccag
      421 ccaccaaaaa cataaacagc aaagaaaggt caaagcttgt tcgaaaaata tgtcgaacca
      481 tagccttatc ctgtctgtgt tttgctgtaa ggatgattgt agcggtagca atccctaaag
      541 ccggcatata gttaaactgc gtcagggttt ctccaattgc atttccagct accgtcgctg
      601 ttcctaagct ggtaatcaaa gctacgacta ccacatctcc cacccgcatc atcaaccgtt
      661 caccagttgc tggcagggca agtctaagca gttctttatc aaaggaccag gtccatggtt
      721 ttaaagaaat tggtaagcga gaccaaagca ggctacaacc aatcaaccgc gataataccg
      781 tgccaatcgc aactccagct acacctgcat gaagcacaaa gactgcaaat gctgaaaata
      841 gtgcatttag aacgtttgag agcaggctga tatacatagg aaaacgtggt tcccccaagg
      901 ttcgtaagat tgcactcaaa cttgtcatga gtcccaaaaa aggtgctccc ccaccaacca
      961 atatcaaata aagacctcca gcctgtacca cttctgactc tgcccccaag ctagtaagta
     1021 ctattggacc tgccagaact gaaaaaattc ctaaaaggag accgataaaa actgtaaact
     1081 tgacactttc actagcagtt cctccaatac cttctgaacc cccttcaccc aaagcctgag
     1141 ccaaacgaga agacaccgct gctgctaggg caataaaaac cgcctgatag acggctaaaa
     1201 tattattagc aagggaaact ccagacaaag ctacgatgcc aatactggct actagataag
     1261 agtctactac ccccattaac atctggagaa ggttttcccc catggctgga aaggcaatgg
     1321 ttaaaatttc tttataggtt tgtttgttca taatctctat tccaactgtt taata
//
LOCUS       DATUCG010000233         1365 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.236, whole genome shotgun sequence.
ACCESSION   DATUCG010000233 DATUCG010000000
VERSION     DATUCG010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.236"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            1..1126
                     /locus_tag="VCA20_002413"
                     /pseudo
     CDS             1..1126
                     /locus_tag="VCA20_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608510.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
BASE COUNT          529 a          187 c          244 g          405 t
ORIGIN      
        1 gtatcatcaa ccatgagatg atgtggttac cgaacaagtc tattattgta agagatgatc
       61 gaaacgagga aatagaatta caaagcattg ctgataaatt aagatttcag cctgaacaag
      121 ttacttttac tatagctccc acaatgaatt gtaattttaa ttgttcttat tgctatgaaa
      181 aaggatttag atgttattct atgaaggatg aggtggcaga aaaagtagct tattttattc
      241 ttagtaatat caagcaagaa caatcactaa aaataatttg gtacggtgga gaacctctaa
      301 tgggaataaa agcaataaag attattagtg aaataatcat tgcaaatcaa ttgttgtaca
      361 aagagtttgt ggcggaaata gtcactaatg gatactacct aacgaaaaat atggcaaaat
      421 tattaaaatc tttgcacgta aattatgtac aagttactct tgatggggat aaagaaacac
      481 acgataaaag gaggtgtctg gttgatggca caccaacttt tgatagaatt ataaaaaaca
      541 tacaaaatac tttcgaaatt attcagacaa ctataagaat aaacatagat aagaataata
      601 acgcaaaacc agtcttaaac ttaatggaaa cattacataa aaaaacttac taaattgtgt
      661 aggattttat ttagcaccag ttgaggaatt tgataatgtt gttaatccta cttgtctaaa
      721 tccgagagaa ttttcaatag aagaattgaa aatatataaa gagctattta gtagaggaat
      781 aaatattaca aaattacctc gaacgacact tggtttttgt gaagcaataa atagagatga
      841 ttatgtaata gatccaaatg gttttttgta taaatgttgg gtgcacattg gtaagcagac
      901 gggaagagtt ggaagtattt ttagcggaat agatacgaat atgttttatg aaaagtttac
      961 aaacttgagg ttaatggaag atccaaaatg caaatcatgc aatgtccaac cgctttgctt
     1021 aggtggatgt ccgttaataa aaatgaacac aggaaattcc aaatgtgtat cgctgaagtt
     1081 taatatttct gaacaacttc aaatactggc aaaagttgtg gaatagatac ttgacatata
     1141 aatctctatt actgatatgg attaatttct aaagacataa aacaaacagt aacaggaatg
     1201 cttctatacg ctaaaaccga tatagatatt caacctaatc aggtctacca aatgcatgga
     1261 aaccagatta gtgtcaaaaa tttggactta aacctgcagt ttgcaagtat tgctgaacaa
     1321 ctggatgaca tcattacatc gcattttgca tcccctccaa aacgg
//
LOCUS       DATUCG010000234         1262 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.237, whole genome shotgun sequence.
ACCESSION   DATUCG010000234 DATUCG010000000
VERSION     DATUCG010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.237"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(115..1206)
                     /locus_tag="VCA20_002414"
     CDS             complement(115..1206)
                     /locus_tag="VCA20_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HEP1836118.1"
                     /translation="MVHNVQNYRDLIAFHPGSYVEEIIEDLNISQEEFAIRLGTTPKT
                     ISKLVNGEISVSKDLANKLFKLTGVSILTWLNLQASYDAKVLEIEELQNQDEKEICQL
                     IDFDYFKRFGFVPPGRYTLLQKVAELRKLFQISNLSYLTQFNQLVSYRNTKGFDERSV
                     VNSNVMLELAIKLSKDATDTKFNKSKLVKILPELREMTLKDGGEFYPMLHSKLLECGI
                     CLVGLPKLKNANLNGATKRFKNGSVLLLLTDKNKRSDIFWFSFFHELGHILHNDFYSD
                     YDDRDAYEEKEQKADQFAQEFLIPTKEYEKFIAEKSFDKSSIIRFAHEIGIHPSIVLG
                     RLQKEGFVEYSQYQELKISYNIIINGNLM"
BASE COUNT          409 a          239 c          179 g          435 t
ORIGIN      
        1 tacagtatag cataaaatga caatttacag gaggactgac ctgtgtatcg actcacatat
       61 tgaacacaag aaaaacgagg atttttcaac cctcgtgcac taaccaattt caatttacat
      121 caaatttcca ttgattatta tgttgtaact aatttttagc tcttgatact gactgtattc
      181 tacaaatcct tctttttgga gtcttcccaa aacaatactt ggatggatac cgatttcatg
      241 agcaaaacga atgatggatg atttatcaaa agatttttcg gcgataaact tttcgtattc
      301 tttcgtagga attagaaatt cctgagcaaa ttgatccgct ttttgctctt tttcttcata
      361 agcatcccta tcatcataat ccgagtaaaa atcattatgt aaaatatgac ctaattcatg
      421 gaaaaatgag aaccaaaaaa tatctgaacg cttattttta tcagttaaca acaataatac
      481 actaccattt ttaaatcgct tggtcgcacc attcagattg gcatttttta attttggaag
      541 tccgaccaaa caaataccac attccaataa ttttgaatgt aacatcgggt aaaattctcc
      601 accatctttc aaggtcatct ctcgtagttc tggtagtatt tttactaatt ttgacttatt
      661 aaactttgtg tcagttgcat ctttggataa ttttatagcc aactcaagca taacatttga
      721 atttaccacc gaacgctcat caaaaccttt tgtatttcga taactaacaa gctgattgaa
      781 ctgcgttaga taggaaaggt ttgaaatttg aaaaagcttt ctcaactcag ccactttttg
      841 tagcaaggta tacctgcccg gtggaacaaa gccaaatctt ttaaagtaat caaaatcaat
      901 caactgacaa atctcttttt catcttgatt ttgaagttcc tctatttcaa gaacctttgc
      961 atcatagctc gcttgaagat ttaaccaagt aagaattgat acccctgtta atttaaatag
     1021 tttattagca agatccttac tgaccgaaat ttcaccattc actagtttac tgattgtttt
     1081 tggggttgtc cctaaacgaa tcgcaaactc ttcttgggaa atatttaagt cttctattat
     1141 ctcttcaaca taagaacctg gatggaaagc gatcaaatct ctatagtttt gaacgttatg
     1201 aaccataatg attacttacc tcctcaattt gaattatttt aatactctgg gcattggaaa
     1261 at
//
LOCUS       DATUCG010000235         1256 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.238, whole genome shotgun sequence.
ACCESSION   DATUCG010000235 DATUCG010000000
VERSION     DATUCG010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.238"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            18..>1256
                     /locus_tag="VCA20_002415"
     CDS             18..>1256
                     /locus_tag="VCA20_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HEP1836119.1"
                     /translation="MLHKEKPDYNRNQYGFYTLDQLVPADHFLRQVEAVIDFDFLYNL
                     VEDTYSSDNGRPSLDPVMLVKIPLIQCFYGIRSMRQTIKDIEVNTAYRWFLGLTLDDK
                     VPHFTTYGKNYSRRFQEKGLIESIFTHILGLCINAGLIDPTEIFIDGTHIKAAANNRK
                     FINQEVEKQAKFMSEQLEIEINQDRVKHGKKPLGPAKEPEPIAKKISTTDPESGWFHK
                     GDHKEVFAYSAQVACDKYGWALAYSVEAGNIHDSQAFPALFAKLEPLKPEYIIADSGY
                     KTPSIAKFLIDKEITPVLPYTRPRGKKGQLRPKDFVYDEHFDCILCPEHQALTYRTTT
                     REGYREYKSDPAICANCPLLSVCTTSKNHQKVVTRHIWKEYLEQCEDIRHQRGMKELY
                     QHRKETIERLFGTAKEYHNLR"
BASE COUNT          358 a          287 c          276 g          335 t
ORIGIN      
        1 acttatgagg ttctcctatg ctacacaaag aaaaacctga ctataaccgc aatcagtacg
       61 gtttctatac ccttgaccag cttgtgcctg cagatcattt cctgcgccaa gtggaagctg
      121 tgattgactt cgacttcctt tataatctag tagaagatac ctattcgtct gataatggtc
      181 gtcctagtct tgaccctgtc atgttagtta aaattcctct gattcagtgc ttttatggta
      241 ttcgttccat gcgtcagacc atcaaggata ttgaagtgaa tactgcctat cgctggtttc
      301 ttggtttaac cctagatgac aaggtgcctc acttcacgac atacggcaaa aattattccc
      361 gtcgctttca agagaaaggg ttgattgagt ccattttcac gcatattctg gggttgtgta
      421 tcaatgcagg cctgattgac ccgacggaga ttttcataga cggaactcat attaaggctg
      481 cggctaataa tcgtaagttt atcaaccaag aggtggaaaa gcaggccaaa ttcatgagtg
      541 aacagctgga aatcgaaatt aatcaagatc gggtaaagca cggaaaaaag ccgctcgggc
      601 ccgccaaaga gccagagccc atcgctaaga aaatctctac aaccgaccca gaaagtggct
      661 ggttccacaa aggtgatcat aaggaagttt tcgcttattc tgcccaagtt gcctgtgata
      721 agtatggctg ggcacttgct tatagtgttg aggctggtaa tatccatgac agtcaggctt
      781 ttcctgcact tttcgccaag cttgaaccct tgaaaccaga gtatatcatt gcggattcgg
      841 gctataaaac tcccagtatt gccaagtttc taattgataa ggaaatcact cctgtccttc
      901 cctacacccg tccccgtggg aagaagggac aacttcgtcc taaagatttt gtctatgacg
      961 aacacttcga ttgtatcctg tgtcctgaac atcaggcctt gacctatcgc acgactaccc
     1021 gagaaggcta ccgagaatac aaaagtgatc ctgcaatttg tgccaactgt cccctattat
     1081 cagtatgtac gaccagtaag aaccatcaaa aagttgtcac gcggcatatt tggaaggagt
     1141 atttggaaca gtgcgaggat attcgtcatc aaagagggat gaaggagctc taccaacatc
     1201 ggaaagagac gattgaacgg ctatttggca cagccaagga ataccataat ctgcgc
//
LOCUS       DATUCG010000236         1236 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.239, whole genome shotgun sequence.
ACCESSION   DATUCG010000236 DATUCG010000000
VERSION     DATUCG010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.239"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(<1..386)
                     /locus_tag="VCA20_002416"
     CDS             complement(<1..386)
                     /locus_tag="VCA20_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEP1836120.1"
                     /translation="MYNKTILIGRLTAQPELTQTPTGKNLTRVTVAVNRRFKTENGER
                     EADFLNVIFWGKLAETLVSYGSKGSLISIDGELRTRKYEKDGSNHYVTEILGNTFQLL
                     ESRAQRAMRENNTGDDLADLVLEEEE"
     gene            441..1220
                     /locus_tag="VCA20_002417"
     CDS             441..1220
                     /locus_tag="VCA20_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HEP1836121.1"
                     /translation="MKEFLMEKIQTSQPYTNAELAWLLENIGHPDPTIRDELVYASFC
                     HIFLEGLITREQAQSLLQFSQETKPFSLEGSTQKRSFICLLYCLLLSVDNESESIYHT
                     FLSTNDRELLFQQALDYLAIENDWSGYDEKLGWIHTAAHGADFLLAASCHDQFPTEKS
                     KEVWQTIVTCLTKQSKVFSAGEEIRLAQIPVYLLLNEKVSSQELTEWISKLDFPNQEP
                     VDYFRWLNLQHFLSSLYFQLRSHQASTEEIEQAIEGKIEPA"
BASE COUNT          355 a          286 c          248 g          347 t
ORIGIN      
        1 aattcctctt cttccaagac caagtcagct aggtcatcgc ctgtgttatt ttcacgcatg
       61 gcacgttgag cccgactttc gagcagctgg aaggtattgc ccaagatttc agtcacatag
      121 tggttgctgc cgtctttttc gtattttcgc gttcgcaact caccgtcaat agaaatcaga
      181 ctacccttgc taccatagga gacaagtgtt tccgccagtt tgccccagaa aataacatta
      241 agaaaatctg cttcacgctc accattttca gtcttaaatc ggcgattgac tgcgactgtt
      301 acacgagtca aatttttgcc agttggtgtt tgagtgagtt caggttgggc cgtcaagcga
      361 ccgattaaaa ttgttttatt atacatatta tatcctccta cttatttatt cgtagaagga
      421 ttagaaaagg agcgaaaatc atgaaagaat ttttgatgga aaagatacag acctcccaac
      481 catataccaa tgccgaactg gcttggttgt tggaaaacat cggccatcca gaccctacta
      541 tacgcgacga actggtctat gctagttttt gccatatctt tttggagggc ttgattacaa
      601 gagaacaagc acagtcgcta ctgcaattct ctcaagaaac caagccattt tccctagaag
      661 gctccacaca aaaacgaagt tttatttgct tactttactg tctgttgctg tctgtcgata
      721 atgagtcaga gtctatttat catacatttt taagcactaa cgaccgtgag ctactcttcc
      781 agcaggcctt ggattatttg gcgattgaaa atgactggtc tggctacgat gaaaaactcg
      841 gttggatcca taccgctgcc cacggagcag attttcttct ggccgcaagc tgtcatgatc
      901 aatttcccac tgaaaaaagc aaagaagtct ggcaaactat tgtcacttgt ctaactaaac
      961 aaagtaaagt tttctcagct ggcgaggaaa tccgtctggc acaaatccct gtctacctcc
     1021 tgctgaatga aaaagtgtca agccaagagc tgacggaatg gattagcaag ctggactttc
     1081 caaatcaaga accagtagac tacttccggt ggctcaatct ccagcatttc ctgtccagtc
     1141 tttatttcca acttaggtca catcaagcat cgaccgaaga aatcgaacag gctattgaag
     1201 gaaaaattga accagcataa aagaattggc cacctg
//
LOCUS       DATUCG010000237         1172 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.240, whole genome shotgun sequence.
ACCESSION   DATUCG010000237 DATUCG010000000
VERSION     DATUCG010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.240"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            1..1044
                     /locus_tag="VCA20_002418"
     CDS             1..1044
                     /locus_tag="VCA20_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEP1836122.1"
                     /translation="MINALDLFCGVGGLTYGVQNAGINVVAGFDFEKSCQFAYEKNNN
                     SKFIHKDIKELQDGELLGYYPEETDVKVLIGCAPCQPFSAYSHRYKGSSASEGKLDLL
                     DYFGKQIRQVKPEIVSMENVPQLAKEDIFDRFVTLLEDEGYKVTWKIAYAPAYGIPQN
                     RKRLLLLASKFGEINFDDELSENDYPTVRDAIYHLPKLQAGQIDDKDPLHRARNLNAI
                     NLKRIRSSKPGGTWKDWDESLLPDCYKKKSGKSFGSVYGRLEWDKPSSTITTQFPGYG
                     NGRFGHPEQDRALSLREGAILQSFPMEYQFVENVGSFSSQKIAMQIGNAVPPKLGEVI
                     GNSILKHLEEYYG"
     gene            1037..>1172
                     /locus_tag="VCA20_002419"
     CDS             1037..>1172
                     /locus_tag="VCA20_002419"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HEP1836123.1"
                     /translation="MAKQLGLFDDEKKLEIQNQINELQYELNYDTRDYPIGYIVDLYD
                     G"
BASE COUNT          417 a          156 c          248 g          351 t
ORIGIN      
        1 ataataaatg cattagattt gttctgtgga gtaggagggt tgacttacgg tgttcaaaat
       61 gctggcatca atgttgttgc tggatttgat tttgaaaaat cttgtcaatt cgcttatgaa
      121 aaaaataata attctaaatt cattcataaa gatattaagg aattacagga tggagagtta
      181 ttgggatatt atcctgaaga gacagatgta aaggttttga taggttgtgc tccatgtcag
      241 cctttttctg cgtattctca ccgatataaa ggtagctctg ctagtgaagg aaagttagac
      301 ttattagatt actttggaaa acaaattcgt caagtaaagc ctgagatagt ctcaatggaa
      361 aacgttcctc agttagcaaa agaagatatt tttgataggt ttgtaacttt gttggaagac
      421 gagggttata aagtaacatg gaaaatcgct tacgcaccag cttacggaat tccccaaaat
      481 agaaagaggt tattactact agcttcaaaa tttggagaaa tcaactttga tgatgagtta
      541 tcagaaaatg attatccaac agttagggat gcaatttatc atttaccaaa actgcaggca
      601 ggacaaattg acgataaaga tccattacat cgtgctagaa acttaaatgc tataaattta
      661 aagagaatca gaagttcaaa gcctggagga acatggaaag attgggatga atcactttta
      721 cctgactgtt ataaaaaaaa gagtggaaag agttttggtt ccgtctatgg tagattggag
      781 tgggataaac cttcatctac aataacaact cagtttccag gatatggtaa tggtcgattt
      841 ggacaccccg aacaagatag agctttgagc ttgagagagg gagctattct tcaaagtttt
      901 cctatggaat accaatttgt tgaaaatgtt ggaagttttt caagtcagaa aattgcaatg
      961 caaattggta atgcagtacc tccgaaacta ggagaagtga taggaaacag tatattaaaa
     1021 catttggagg agtattatgg ctaaacaatt aggattattc gatgatgaga agaagttaga
     1081 aattcaaaat caaataaacg aattacaata cgaactcaat tatgatacac gagattatcc
     1141 tataggttat atagttgatt tatacgatgg ta
//
LOCUS       DATUCG010000238         1127 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.241, whole genome shotgun sequence.
ACCESSION   DATUCG010000238 DATUCG010000000
VERSION     DATUCG010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.241"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            154..1127
                     /locus_tag="VCA20_002420"
                     /pseudo
     CDS             154..1127
                     /locus_tag="VCA20_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015445251.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT          347 a          212 c          256 g          312 t
ORIGIN      
        1 tgtgataatt ggcgtatact ccattgcaaa tgatgagatt tagcggtata ctgtaagtag
       61 ctcatttgga ctaacctcct gtatgtttcg tcacttacag tatagtccat ttgggctttt
      121 tattgtagtt tgaaatgaac tagcaccacg ggtgttagcc atattgtgaa ctatgtgaat
      181 gaggttcgtc gtttatatgg tcaagatgca gaagtgttat gtggctacaa agccggatgt
      241 cttggattta ccctatatca ccagctacaa gctcacggga ttccctgtat cgtgatggcg
      301 cctacaacgg tgatgaagga aggatctaag cgtgttaaga ctgataaaaa agatgcagct
      361 cagctcgtaa aagctctggc ctttcgtagc tatcggcctg ttcatattcc tactgttgag
      421 gatgaacaag tcaaagaata tatccgcatg agaacagacc acaaagtggc tctgaagaaa
      481 atcaaacaac aaattcttgc cttctgtctg cgacatgatt ttctctatac cgagggaagc
      541 agtaattgga cacagaaaca tgtccgctgg ctccattccc taaatcctga tggactttac
      601 gcagagattt tgacagaata cctattgacc tatgagaaat tagtagatca aatagaacgg
      661 tatgatgcgc gaattgagca actgggtcaa agcgacagtt atcaagagaa ggtctcacgg
      721 ctctcttgct ttattggcat taaaacacta accgcacttt ccattatgac ggaaatcggt
      781 gattttaacc gctttgcgac agctcaacat tttgcttctt atcttaggct aactcctagc
      841 gaaaattcta gcggcgacaa ggagagaaga ggtgctatca ccaaaggtgg taatagccat
      901 gtgagacgac ttctgataga agctgcacaa tcattggcta aggggactat tgggtataaa
      961 tccaaagaat tgaaaaggag acaaagtgga aaccgagtgg aggtgattgc ttatgcggat
     1021 aaggctaatg aacgcttaag aagacgttat cgcacacttg ttctaggaaa aaataagaaa
     1081 caaaatgttg ctaaaacata ttgcacgaga attatctggt tttattt
//
LOCUS       DATUCG010000239         1121 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.242, whole genome shotgun sequence.
ACCESSION   DATUCG010000239 DATUCG010000000
VERSION     DATUCG010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.242"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            111..737
                     /locus_tag="VCA20_002421"
                     /pseudo
     CDS             111..737
                     /locus_tag="VCA20_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775244.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
BASE COUNT          348 a          198 c          276 g          299 t
ORIGIN      
        1 ccacttttga gttaccgaac aagtctaata gtattagcat agtaaaggat gtaaaaaatg
       61 aaaagaacag aacgaggtca gatttgctag gcctctccag aggaaagaaa atgacagatt
      121 atgtaaacta caaccaagaa cgttggaatc gggtttcagc tagacaggga aatgcttata
      181 ccgttccact cagtcatgag gaatttcttg ttgccaaaga taagccttta tccgtttctt
      241 tgacggttgg aaagactgtg ccgcttgact ggtttgaaaa ggcccaaggc aagaaattgc
      301 tgggtctagc atgcggtggt ggtcaacagg gaccaatttt tgcggctcat gcctatgaga
      361 caactatttt ggacttctcc aaggaacagt tggacaagga taggctagtt gctgagcgag
      421 aaaatctgga tttgcagacg gtgcaggcag atatgacgca agcctttcct tttgaagacg
      481 agagttttga catcatcttt tgtccggttt caaatgttta tatcgaagat ttgaccaata
      541 tgtggcagga gtcttatcgg gttttgaaaa agggcggact gctcatggtg ggttacatga
      601 acccttggat ttatgtcttt gatgctgatg atgtttggga gcatccagac agagcattgg
      661 ttccaatgta tagcctactg ttcaacgcac gaaaattaga agaagcagga caaattacga
      721 ttgacccaga atatggcgtg cgacaagaag tcgtgttata aattgctagt ttagctactc
      781 tatccatttg gggtaatcct accacagcct gcttgactgg taacggttat gttgggaagc
      841 ctgtgggaca atatggaaag tctgatagcc taaaatcgga tggactgtta ggataagaat
      901 actatggtga acagacgtga accatcgcaa gagtcgttac agggactaag tgaaataagg
      961 ctgatacagt tacaccaaaa ggtacggagg aggtcattgg ggctttcata acctgatgac
     1021 aaatggatac attcttcccg gatcaaagat ggcacctaag gtattcatac ttaacaaaaa
     1081 tcataaatag cgatataata gtttcatgac aaaagaactt a
//
LOCUS       DATUCG010000240         1091 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.243, whole genome shotgun sequence.
ACCESSION   DATUCG010000240 DATUCG010000000
VERSION     DATUCG010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.243"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            303..1078
                     /locus_tag="VCA20_002422"
                     /pseudo
     CDS             303..1078
                     /locus_tag="VCA20_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT          397 a          187 c          220 g          287 t
ORIGIN      
        1 atcaatgaac ttgttgcact tgacttgaca acgcggggtg ctatgaaagt tctataatga
       61 agttagccac tttaccttaa tcaaaatcag ctactttact atatatgagt tggcgattga
      121 tttcatttga taacaggttt gaagctgtta ggctaagtag ccaaggctaa tagtagcctt
      181 ggtttagctg acaaaacagt ttcaagggtt cctgttgtca aaggaaatcc attttaaggt
      241 ataagaaaac acctctgtgc tatacttgta gttcaccaca aacacaagaa agaggcacag
      301 agatgcaaaa ctattataca ccaaaaggca aacatttgac aattgataac cgccgcctaa
      361 ttgaaaagtg gaagattgag ggtaagtcta atcgtgaaat tgccgactta cttgaaaagg
      421 ctcctcagac cataaataac gaaatcaatc ggggtacaac tttacaacaa gtgcgaaaag
      481 ggctgtacaa aaaagtctat tctgctgact atgcacaaac ggtataccaa actaatcgaa
      541 aacgatcggt aaaaaagata atcctaacga aagaattgaa agagaaaatt ttacactata
      601 gtaagtaaaa atgttctcct gaaatgatgg ttaagaagaa gaaaatgaaa gtgggtattt
      661 caaccattta ctattggttt cacaatggac acttaggatt gaacaaaaag gatatgcttt
      721 atcctagaaa agggaaaagg gaaaagggaa aagtgttaag aagcaagcca gtcctaactt
      781 taagccggtt ggtaaatcca ttgaagaacg tcctgatatt atcaatcctc gtttggaaaa
      841 tggtcattat gaaattgata cagttctact aaccagaggg aaaaatcatt gccttttagg
      901 cttaaccgac cgtcggagta gacactaaat catccgtcta atacctaaca aaaccactga
      961 atcagtcaat caggcactag agggaatctt gagggaacat cgtatcctgt ctataactgc
     1021 agatagtggt acagaattta gtcgtctggc tgaagtgttt cctgaggagt atatctatag
     1081 ttaaatgaaa c
//
LOCUS       DATUCG010000241         1086 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.244, whole genome shotgun sequence.
ACCESSION   DATUCG010000241 DATUCG010000000
VERSION     DATUCG010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.244"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            <1..390
                     /locus_tag="VCA20_002423"
     CDS             <1..390
                     /locus_tag="VCA20_002423"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1836124.1"
                     /translation="VTEQVYYLPRYLKFKLVNRIMELTSNELLATYAVEDIAKFEEKA
                     SSDRVSVLVNHLDVLENGFLIPGLSMVTSVLVFTFSLIGALYLQGTMTIIMLVLCFIP
                     FLAPLINNKILAHTTKESQEEKNIYLK"
     gene            604..>1086
                     /locus_tag="VCA20_002424"
     CDS             604..>1086
                     /locus_tag="VCA20_002424"
                     /inference="COORDINATES: protein motif:HMM:NF012235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEP1836125.1"
                     /translation="MLKISDLIAMTTLMGTVAGPIQTMSGLITEYLASRTVVEELTTI
                     LKGQSHEDKVQQELTETIRTLTLDRVSSHNQQHPIFDNFSYQFLADKKYAIVGKSGSG
                     KTTLLRLLLGVQSPDEGAIFINQINLSEVAQASYFDKMYYLPQKTAIFATTIGDNLTL
                     F"
BASE COUNT          351 a          181 c          206 g          348 t
ORIGIN      
        1 gttaccgaac aagtctatta tttaccacgc tatctcaagt ttaaattggt caatcgtatc
       61 atggaattga caagcaatga gttgcttgca acctatgcag tagaggatat tgctaaattt
      121 gaagaaaaag caagctcaga tagggtaagt gttttagtga atcatttaga tgtattagaa
      181 aatggctttt taataccagg tttatcaatg gtaacgagcg tccttgtatt tactttctcc
      241 ttaattggag cactttattt gcaaggaaca atgactatta tcatgttggt gctttgcttt
      301 atccctttct tagcaccgtt gataaataat aaaattttgg ctcatacgac taaagaaagt
      361 caagaagaga aaaatatcta tttaaaatga tttagtgagt ttgtacaagc attttctttc
      421 atacgtatca gtaatattac gccagtcttt gaaaagaacc tagagacttc tagtcaagaa
      481 tacgctaaaa gagccatcct attttccaaa aagcaatctc aaacctatgc agtatcttac
      541 ggtctgagta gtattgtcta ttctggttca tggattatta gaggtatctt tgtttttcaa
      601 gggatgctga aaatttcaga cttgattgct atgacgacac taatgggaac ggttgctggt
      661 cccattcaaa ccatgtcagg actgattaca gagtatcttg ctagtcgaac ggtcgtggaa
      721 gaattgacga ctattctgaa aggacaatca catgaagaca aagtccaaca agagttgaca
      781 gaaactataa ggactttaac tttagatagg gttagttccc ataatcaaca acaccctata
      841 tttgacaact tttcttatca atttcttgct gataaaaaat atgctatcgt tggtaaaagt
      901 ggtagtggga agacgacatt gctgagactc ttgctaggtg ttcaatcacc agatgagggg
      961 gcgattttca taaatcagat taatctttca gaagtggctc aggcttccta ttttgataag
     1021 atgtattatc taccacaaaa aacagctatt tttgcaacaa ctataggaga taacttgaca
     1081 ctgttt
//
LOCUS       DATUCG010000242         1084 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.245, whole genome shotgun sequence.
ACCESSION   DATUCG010000242 DATUCG010000000
VERSION     DATUCG010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.245"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(<1..206)
                     /locus_tag="VCA20_002425"
     CDS             complement(<1..206)
                     /locus_tag="VCA20_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEP1836126.1"
                     /translation="MVNCEPLSAYRQLDKAKLVGCWAHVKRKFFEATPKKTDKTSLGA
                     KGLAYCDRLFALENDWADLSTEER"
     gene            complement(279..1001)
                     /locus_tag="VCA20_002426"
     CDS             complement(279..1001)
                     /locus_tag="VCA20_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEP1836127.1"
                     /translation="MTYPVETETITYKSKKTKGVRQAIFSQFTPEIIHHELQGEGCNC
                     PDCHGQLKEIGSTVQRQELVFIPAQLKRIDHVQHAYKCQACSQKNLSDKIIKAPVPKA
                     PLAHSLGSASIIAHTIHQKFNLKVPNYRQEEDWNKLGLPISRKEIANWHIKSSQYYFE
                     PIYDLLHEKLLEQPILHADETSYKVLENDSQLTYYWTFLSGKHEKKGITLYHHDKRRS
                     GLVVEEVLGDYAGYVHCDMLRQ"
BASE COUNT          279 a          266 c          226 g          313 t
ORIGIN      
        1 aatcgttcct cagtagacaa gtctgcccag tcattctcca aggcaaacag gcggtcgcaa
       61 taagctaaac ccttagctcc taaagaagtc ttgtctgtct tcttaggagt cgcctcaaag
      121 aactttcgtt tgacgtgagc ccaacagcca accagtttag ccttatctaa ttgacgataa
      181 gctgacagcg gttcgcagtt caccaagctt agtgtcgctt cgctcctaaa ccttggactg
      241 ctatcgcata ggcagaacta aaggactaaa gtcctaagtt actgccgtaa catgtcacaa
      301 tgtacgtagc ccgcatagtc cccaagaacc tcctccacaa ccaagccact ccgtcgtttg
      361 tcatgatgat agagtgtaat tccctttttc tcatgcttcc cagacaagaa agtccagtag
      421 tatgtcaact ggctatcatt ttctaagacc ttgtaggagg tctcatccgc atggagaata
      481 ggctgctcca acaatttctc gtgcaacagg tcataaatcg gctcgaaata atactgacta
      541 gacttgatgt gccagttggc tatttccttc cgactgattg gcaagccaag cttgttccaa
      601 tcctcttcct gacgataatt gggtaccttc agattgaatt tctgatgaat ggtgtgggcg
      661 atgattgagg ctgaacccaa gctatgtgcc aaaggggcct taggaacggg tgccttgata
      721 atcttatcgc tcagattctt ctgactacat gcctgacact tgtatgcgtg ttgaacatgg
      781 tcaatccgct ttaattgtgc gggaatgaag accaactctt gtcgttgaac agtagaacca
      841 atctctttca actgtccatg acagtctgga caattgcagc cctcaccctg caattcgtga
      901 tgaataatct ctggagtgaa ttgactaaaa atagcctgac gaactccttt agttttcttg
      961 cttttatagg taatcgtttc tgtttcaact gggtaagtca gctgagcttc ttcagggagg
     1021 ttttcctccc caaacaagct cagctgaccg ggttgatata caactttctc tgatgatttt
     1081 ccgt
//
LOCUS       DATUCG010000243         1039 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.246, whole genome shotgun sequence.
ACCESSION   DATUCG010000243 DATUCG010000000
VERSION     DATUCG010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.246"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(<1..206)
                     /locus_tag="VCA20_002427"
     CDS             complement(<1..206)
                     /locus_tag="VCA20_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEP1836128.1"
                     /translation="MVNCEPLSAYRQLDKAQLVGCWAHVKRKFFEATPKKTDKTSLGA
                     KGLAYCDRLFALESDWADSSSEERL"
     gene            complement(279..956)
                     /locus_tag="VCA20_002428"
     CDS             complement(279..956)
                     /locus_tag="VCA20_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEP1836129.1"
                     /translation="MTYPGETETITYKRKKTKGVRQAIFSQFTPEIIHHELQGEDCNC
                     PDCHGQLKEIGSTVQRQELVFISAQLKRIDHVQHAYKCQACSQKNLSDKIIKAPVPKA
                     PLAHSLGSASIIAHTIHQKFNLKVPNYRQEEDWHIKSSQYYFEPIYDLLHEKLLEQPI
                     LHADETSYKVLENASQLTYYWTFLSGKHEKKGITLYHHDKRRSGLVVEEVLGAYAGYV
                     HCDMLRQ"
BASE COUNT          266 a          261 c          225 g          287 t
ORIGIN      
        1 agtcgctcct cgctagacga gtcagcccag tcactctcca aggcaaacag gcggtcgcaa
       61 taggctaagc ccttggctcc taaagaagtc ttgtctgtct tcttaggagt cgcctcaaag
      121 aactttcgtt tgacgtgagc ccaacagcca accagctgag ccttgtctaa ctgccgataa
      181 gctgacagcg gttcgcagtt caccaagctt agtatcgctt cgctcctaaa ccttggactg
      241 ctatcgcata ggcagaacta aaggactaaa gtcctaagtt actgccgtaa catgtcacaa
      301 tgtacgtagc ccgcataggc cccaagaacc tcctccacaa ccaagccact ccgtcgtttg
      361 tcatgatgat agagcgtaat tccctttttc tcatgcttcc cagacaagaa agtccagtag
      421 taggtcaact ggctagcatt ttctagaacc ttataggagg tttcatccgc atggagaata
      481 ggctgctcca acaatttctc gtgcaacagg tcataaatcg gctcgaaata atactgacta
      541 gacttgatgt gccagtcctc ttcctgacgg taattgggca ccttcagatt gaatttctgg
      601 tgaatagtgt gggcgatgat agaagctgaa cccaagctat gtgccaaagg ggccttagga
      661 acgggagcct tgataatctt atcgctcaga ttcttctgac tacatgcctg acacttgtat
      721 gcgtgttgaa catggtcaat ccgctttaat tgtgcggaaa tgaagaccaa ctcttgtcgt
      781 tgaacagtag aaccaatctc tttcaactgt ccatgacagt ctggacaatt gcagtcttcc
      841 ccctgcaatt cgtgatgaat aatctctgga gtgaattgac taaaaatagc ctgacgaact
      901 cctttagttt tcttgcgttt ataggtaatc gtttctgttt cacctgggta agtcagctga
      961 gcttcttcag ggaggttttc ctccccaaac aagctcagct gaccgggttg atatacaact
     1021 ttctctgatg attttccgt
//
LOCUS       DATUCG010000244         1032 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.247, whole genome shotgun sequence.
ACCESSION   DATUCG010000244 DATUCG010000000
VERSION     DATUCG010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.247"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            187..1032
                     /locus_tag="VCA20_002429"
                     /pseudo
     CDS             187..1032
                     /locus_tag="VCA20_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564846.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT          329 a          195 c          240 g          268 t
ORIGIN      
        1 tacaagtcac cgagcgaatt tcttttgggt ggctaacttc aacttgaaat ttggcaacgc
       61 gggacgctac acttttaaaa aaagaaagtg acacgccaaa cgcatgaaga aaaaatcgtt
      121 ttcagaaaac gaaaatcata accaaacatc aatcaaagta gtactctaga atagaggtga
      181 actacaatga ttgattttct tttatccata gaagagcata aagaagagta cgatagccgt
      241 caagcatgga aaattcgcta tcccttatcc actattctgt ttctggtttt cacatgtcaa
      301 ttagcagggg ttgaaacgtg gaaagagagg gaagatttca tagaaatgaa cgaagctgtc
      361 ttgggagaat acgttgactt gagtgtcggc tgtccttcac acgatacctt ggaacgtgtt
      421 gtgagtatgg tcaatccaga tttcttgaag gaactgaaac tgtccttcga aacctcttca
      481 gaaacggata atttttccaa actgattgcc gttgacggaa aaacgattcg ggggaatcga
      541 ggaaaacacc aatccccaac tcatattgtc actgcttatg atggtggtac tcggattagc
      601 cttgggcagg tagctgttga ggataaaagc aatgaaatca cagccattcc tcgcctcttg
      661 cgtcagctag accttcgtaa gagtgtagct acgattgatg ccatgggcac ccaagacaga
      721 cattgcggac gtgattatag gaggtaaggg tgattattgc ttggcagtca aaggaaatca
      781 aggcaatctc catgaggata ttgacctcta ttttaatgac gccaaattat tatctaagtt
      841 gtcagagaaa ggctgtcatt atcagaccat tgaaaaggca cgcagtcaga ttgaggtgag
      901 ggactactgg gtttctcacg atgtgaaatg gctgagccaa cggcatccaa aatggaaaaa
      961 acttcgtggg attgggatga ctaagaatac cattgataaa gatgctatca tcactgaaga
     1021 ggtccgttac tt
//
LOCUS       DATUCG010000245         1018 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.248, whole genome shotgun sequence.
ACCESSION   DATUCG010000245 DATUCG010000000
VERSION     DATUCG010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.248"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(<1..679)
                     /locus_tag="VCA20_002430"
     CDS             complement(<1..679)
                     /locus_tag="VCA20_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3991 domain-containing protein"
                     /protein_id="HEP1836130.1"
                     /translation="MTRIKAVKQKAILDVAESLGYSFRRLSGHIYEHPDHDSFRIFAD
                     TNTFKWFSRDIQGDVIDFVQLVAGVSFKEAVSYLETGDFEQTKVIEEVYQPFHYYLHE
                     EPFQQARTYLNEVRGLSDQIINTFGRQGLLAQATYQTESVLVFKSYDHNGILQAASLQ
                     GLIKNEEKHDRGYLKRIMKGSCGHVGISFDIGNPKRLIFCESVIDMMSYYQLHQKQLS
                     DVRLISME"
     gene            complement(719..>1018)
                     /locus_tag="VCA20_002431"
     CDS             complement(719..>1018)
                     /locus_tag="VCA20_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEP1836131.1"
                     /translation="YMLDYRFESASIYVNTESNVALAEVTYQVTYVSDLSEQQQRTTQ
                     TETKTVTLSYSKVSDKLLVNQLTIWNGKLEDMKEATDGANSSIPTIQGTITSENN"
BASE COUNT          293 a          213 c          186 g          326 t
ORIGIN      
        1 cttccattga aatcaggcga acatctgata gctgcttttg gtgaagttga taataactca
       61 tcatatcgat aactgattca caaaaaatga gtcgcttagg attcccaata tcgaaactaa
      121 taccgacatg gccacaagat cctttcatga ttcttttgag ataacctcga tcgtgttttt
      181 cttcattttt gatgagacct tgaaggctag cggcctgtaa aatcccatta tggtcatagc
      241 ttttaaacac taaaaccgac tccgtttgat aagtagcttg agcaagtaat ccttgtctac
      301 caaaggtatt gataatctga tcacttaggc cacgaacttc attcaagtaa gtacgtgctt
      361 gctgaaaagg ttcttcatgc aaataatagt gaaacggttg ataaacctct tctattactt
      421 tggtctgttc aaaatctcca gtttcaagat aagatacagc ctctttgaaa gaaacacctg
      481 ccactaattg aacaaagtca attacatccc cttgtatatc tcttgaaaac catttgaaag
      541 tattggtatc cgcaaaaatc cgaaaggaat catggtctgg gtgttcataa atgtgtcctg
      601 ataaacgtct gaaggaataa cccagactct cagccacatc taaaatggcc ttttgtttta
      661 ctgctttaat tcgtgtcatt ttgtctccct atcaaaaaaa gcagaagact gtctcctgct
      721 agttgttctc gcttgttata gttccttgga tcgttggtat gctggaattg gctccatctg
      781 ttgcttcttt catgtcctcc agtttcccat tccaaatggt caactgatta accaggagct
      841 tgtcggaaac tttagagtag gacaacgtaa ccgtcttagt ttctgtctga gtggttcgct
      901 gttgttgctc actcaaatca gaaacatagg tcacttgata ggtaacttcc gcaagtgcaa
      961 cattgctttc tgtattgaca tagatactgg ctgattcaaa tcggtagtca agcatata
//
LOCUS       DATUCG010000246         1017 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.249, whole genome shotgun sequence.
ACCESSION   DATUCG010000246 DATUCG010000000
VERSION     DATUCG010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.249"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            <1..300
                     /locus_tag="VCA20_002432"
     CDS             <1..300
                     /locus_tag="VCA20_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEP1836132.1"
                     /translation="YMLDYRFESASIYVNTESNVALAEVSYQVTYVSDLSEQQQRTTQ
                     TETKTVMLSYSKVSDKLLVNQLTIWNGKLEDMKEATDGANSSIPTIQGTTTSENN"
     gene            339..>1017
                     /locus_tag="VCA20_002433"
     CDS             339..>1017
                     /locus_tag="VCA20_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3991 domain-containing protein"
                     /protein_id="HEP1836133.1"
                     /translation="MTRIKAVKQKAILDVAESLGYSFRRLSGHIYEHPDHDSFRIFAD
                     TNTFKWFSRDIQGDVIDFVQLVAGVSFKEAVSYLETGDFEQAKLVEETYQPFQYYLRE
                     EPFQQARTYLKDIRGLSDQTINSFGRQGLLAQATYQAESVLVFKSYDHNGILQAASLQ
                     GLVKNEEKHDRGYLKKIMKGSHGYVGISFDIGNPKRLIFCESVIDMMSYYQLNQKQLS
                     DVRLISME"
BASE COUNT          332 a          188 c          213 g          284 t
ORIGIN      
        1 tatatgcttg actaccgttt tgaatcagcc agtatctatg tcaatacaga aagcaatgtt
       61 gcacttgcgg aagtttccta tcaagtgacc tatgtttctg atttgagtga gcaacaacag
      121 cgaacaactc agacagaaac caagacagtt atgttatcct actccaaggt ttccgacaag
      181 ctcctggtta atcagttgac catttggaat gggaaactgg aggacatgaa agaagcaaca
      241 gatggagcca attccagcat accaacaatc caaggaacta caacaagtga gaacaactag
      301 caggagacat tcttctgctt tttttatagg gagacaagat gacacgaata aaagcagtaa
      361 aacaaaaggc cattttagat gtggctgaaa gtctgggtta ttccttcaga cgattatcag
      421 gacacattta tgaacaccca gaccatgatt cctttcggat ttttgcggat accaatactt
      481 tcaaatggtt ttcaagagat atacaagggg atgtgataga ctttgtacag ttagtggcag
      541 gagtgtcttt caaagaggct gtatcttatc ttgaaactgg ggattttgaa caagctaagt
      601 tggtagaaga aacttatcaa ccgtttcaat attatttgcg tgaagaaccc tttcagcaag
      661 cacgtactta cttgaaagac atccgtggac taagtgatca gactatcaat tcctttggca
      721 gacaaggatt gcttgctcaa gctacttatc aagcggagtc agtattagtg tttaaaagtt
      781 atgaccataa tgggatctta caggccgcaa gccttcaagg tctcgtcaaa aatgaagaaa
      841 aacacgatcg aggttatctc aaaaaaatca tgaaaggatc tcatggctat gtcggtatta
      901 gtttcgatat tgggaatcct aagcgactca ttttttgtga atcagttatc gatatgatga
      961 gttattatca acttaaccaa aagcagctat cagatgttcg cctgatttca atggaag
//
LOCUS       DATUCG010000247          999 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.251, whole genome shotgun sequence.
ACCESSION   DATUCG010000247 DATUCG010000000
VERSION     DATUCG010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..999
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.251"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            129..317
                     /locus_tag="VCA20_002434"
     CDS             129..317
                     /locus_tag="VCA20_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEP1836134.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRAVKPNLQKVTVLIDGKP
                     KKVWASARALKSGKVERV"
     gene            711..999
                     /locus_tag="VCA20_002435"
                     /pseudo
     CDS             711..999
                     /locus_tag="VCA20_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001871253.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          350 a          175 c          203 g          271 t
ORIGIN      
        1 aatttttcgt gcgtatgacg tgagaaagtg aaaataattc ttgccaagtc caatcttttc
       61 tgttagaata gtgtggtatg tggtgctaga acatccgata tatttgatca aatgaggagg
      121 aagatacaat ggctaaagta tgttatttca ctggtcgtaa gactgtatca ggtaacaacc
      181 gttcacacgc tatgaaccaa actaaacgcg cagttaaacc aaaccttcaa aaagtaactg
      241 ttttgattga tggtaaacct aaaaaagttt gggcttcagc acgtgcacta aaatcaggca
      301 aagttgaacg cgtataataa caaagtcgtt agaaaaatct agcgactttt gtttttggaa
      361 aaatagccta gatataaagt cggtgaggag caaattcgac cactacataa taaacaggaa
      421 agaatatcag ctacatagct cttcacattg ctgttgaaaa gttaacaaag aaggaacgtt
      481 gtcaaaaata aactgtgtat tgatgtggga gattttctca agctcgaaca tataaacttg
      541 gattgacatt aggaggagca tatctgtacg gattctataa gaagggaatc acgaactgga
      601 agaatagaga gtgtttaaaa aatcccgcgt tgtaaaatga agtgcaacaa aaaagatatg
      661 ttcgtctgat atactagaat tccccaactc agtatagaca gcagatgaac atgtccaaca
      721 ctcattctac cagaaactca tcctattctc atctctcagc cacagaacgt ggtgaaattg
      781 ttgcttatct taaaatggga aagaaaccaa ttgaaatcgc tcggcttctg ggtcgtcacc
      841 ggtctacaat ctgtcgagaa atcaaacgtg gttcagttaa tcaggtacag gataaaatgg
      901 gaaacgaacc tatttcagca cctatttcgc tgatagtgga caaggtgtct atgaaaccaa
      961 tcgtcaaaag tgttcttatc tcaagttgaa tgattgctc
//
LOCUS       DATUCG010000248          982 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.252, whole genome shotgun sequence.
ACCESSION   DATUCG010000248 DATUCG010000000
VERSION     DATUCG010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..982
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.252"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            82..519
                     /locus_tag="VCA20_002436"
     CDS             82..519
                     /locus_tag="VCA20_002436"
                     /inference="COORDINATES: protein motif:HMM:NF024786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S cluster-binding domain-containing
                     protein"
                     /protein_id="HEP1836135.1"
                     /translation="MILSKFVRIIPNTKGYILCHTLNRCIVQIDETHLVNNELVAEKF
                     SKMEVDYLSENDFFSNNPQKYIDATDYSERNITTITINITEYCNFDCTYCYQNDFDSF
                     NVITVELIQKIINYVEYVLRQGEKNIYVYFFGGEPLLFKKKFF"
     gene            531..>982
                     /locus_tag="VCA20_002437"
     CDS             531..>982
                     /locus_tag="VCA20_002437"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEP1836136.1"
                     /translation="MDKLANKFGAYILYGIGTNAYLLDSDFVSQFDQLVVDTTLTLPS
                     DHNKVRTLKGGKSIFDRIFENLKVASKSPNVVLHVGYNTHHGNINDFESFLKVLKDND
                     IKARIGIYYVNNYVFNNDFFNELNFQDFLKWKSSIAISLLIKYDYMVH"
BASE COUNT          374 a          105 c          158 g          345 t
ORIGIN      
        1 attgaattga acaacgtttt atataaatgt tgtcaagctt aaagcaataa taaatataca
       61 aaaaatattt ggagaacgca tatgattctt tcaaagtttg taagaattat tcccaataca
      121 aaaggatata ttctttgcca cacactgaat agatgtatag ttcagataga tgaaacacac
      181 ttggtcaata atgaacttgt agcagaaaag ttttcgaaaa tggaagttga ttatctaagt
      241 gaaaatgact tcttttctaa taatcctcaa aaatatatag atgctactga ttattccgaa
      301 agaaatatta caaccattac aataaatata actgaatatt gtaactttga ctgtacctat
      361 tgttatcaaa atgattttga tagctttaat gtaataacag ttgaacttat ccaaaaaata
      421 ataaattatg tagaatatgt gttacgtcaa ggtgaaaaaa acatctatgt atatttcttt
      481 ggtggagagc cacttttgtt taaaaaaaaa tttttttaat taagagtgag ttggataagt
      541 tagccaataa atttggagcc tatattttgt atggtattgg gactaatgca tatttactgg
      601 attcagattt tgtaagccaa tttgatcaat tggttgttga tacaacattg acattgccgt
      661 cggaccataa taaagtaaga actttaaaag gtgggaagtc aatatttgat aggatttttg
      721 aaaatcttaa agtagcaagt aaaagtccta atgtggtttt acatgttgga tataatacac
      781 atcatggtaa tattaatgat tttgaaagtt ttttgaaagt tttaaaggat aatgacatta
      841 aagccagaat aggaatttat tatgtgaata actacgtgtt taacaatgat ttttttaatg
      901 agttaaattt tcaagatttt ctaaagtgga agtcaagtat agcaatctca ttattaataa
      961 aatacgatta tatggtacat at
//
LOCUS       DATUCG010000249          978 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.253, whole genome shotgun sequence.
ACCESSION   DATUCG010000249 DATUCG010000000
VERSION     DATUCG010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..978
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.253"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(769..>978)
                     /locus_tag="VCA20_002438"
     CDS             complement(769..>978)
                     /locus_tag="VCA20_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1836137.1"
                     /translation="IGYKSKELKRRQSGNRVEVIAYADKANERLRRRYRTLVLGKNKK
                     QNVAKTAIARELSGFILGMMTGRIA"
BASE COUNT          294 a          193 c          180 g          311 t
ORIGIN      
        1 acaaatcgct catataaagc gagaacttgg gtaaagattt ttgaaaaaat aacttgtgtt
       61 tgattgggat ttgcaagata ataatgttgg ctttgcatat cattccctca tttcgtggtt
      121 gttttgagtt tatgatacgc aaagtgtttt tatttaacta gaattaacta gcacaacggg
      181 tgtcttttag tttactttta gtactaggca acgagctgca gacagcttgt acagtcgagt
      241 gactgtgcaa ggttggagat agcacttgcg gagcaagtca cccctgcaga gtacagcaaa
      301 gcgagttcaa acaatccagt ggagtgtttg aagttggaaa taaggaaacg gagtttcctc
      361 ttacgtcgaa gtaataaaag aaaaactaaa cgactgatat gtaaagccct ttgtcaagta
      421 aaaacaatcg aactgataaa aaatgaaaag tatctagaaa gagcctagtt cttagcttcg
      481 ggcattggcc actctgatat tcgtggattg gttacctaca ggtcaatggt tcttccgcga
      541 gtcctactct ctataagcgt ggatcacaat tgtgccactt agttgtttcg tagatgactc
      601 aaaccttgaa gtcctaaact ccttcaagat actttccatt caaacattat ttcaatcctt
      661 agttctttcc aaattcaccc agtgatttgg gatagaaata ggattcctca tcgctctgtg
      721 atgataaaac ttaattgtca aaagtgtaca tgaaaacaag cgctaacttc aagctattct
      781 tcctgtcatc atccccaaaa taaaaccaga caattctcgt gcaatagctg ttttagcaac
      841 attttgtttc ttattttttc ctagaacaag tgtgcgataa cgtcttctta agcgttcatt
      901 agccttatcc gcataagcaa tcacctccac tcggtttcca ctttgtctcc ttttcaattc
      961 tttggattta tacccaat
//
LOCUS       DATUCG010000250          972 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.254, whole genome shotgun sequence.
ACCESSION   DATUCG010000250 DATUCG010000000
VERSION     DATUCG010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..972
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.254"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            <1..>972
                     /locus_tag="VCA20_002439"
     CDS             <1..>972
                     /locus_tag="VCA20_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HEP1836138.1"
                     /translation="QLAGIETWKEMEDFIEMNESVLGEYVDLSAGCPSHDTLERVVSM
                     VNPDFIKELKLSFETSSETTDFSKLIAVDGKTIRGNRGKHQSPTHIVTAYDGGNRIGL
                     GQVAVEDKSNEITAIPRLLRQLDLRKSVVTIDAMGTQTDIADVIIGGKGDYCLAVKGN
                     QGNLHEDIDLYFSDAKLLSKLTAKGCHYQTIEKARSQIEVRDYWVSHDVKWLSQRHPK
                     WKKLRGIGMTKNTIDKGGIITEEVRYFILSFKGDVQTFSQVVRGHWSVESLHWLLDMV
                     YREDKNQTLDKRAAFNLNAIRKVCLHLLQMMTFPKEKLSYRRKQRYI"
BASE COUNT          286 a          183 c          239 g          264 t
ORIGIN      
        1 gtcaattggc ggggattgaa acgtggaaag agatggaaga tttcatagaa atgaacgaat
       61 ctgtcttggg agaatacgtt gacttgagtg ctggttgtcc ttctcatgac accttggaac
      121 gtgttgtgag tatggttaat ccagatttca taaaagaact gaaactgtcc ttcgaaacct
      181 cttcagaaac aactgatttt tccaaactga ttgccgttga cggaaaaacg attcgaggga
      241 accgaggaaa acaccaatct cctactcata ttgtcactgc ctatgatggt ggtaatcgga
      301 ttggccttgg gcaggtagct gttgaggata aaagtaatga aatcacagcc attcctcgcc
      361 tcttgcgtca gctagacctt cgtaagagtg tagtcacgat tgatgccatg ggcacccaga
      421 cagacattgc ggacgtgatt ataggaggta agggtgatta ttgcttggca gtcaaaggaa
      481 atcaaggcaa tctccatgag gatattgacc tctattttag tgacgccaaa ttgttatcta
      541 agttgacggc gaaaggctgt cattatcaga ccattgaaaa ggcacgcagt cagattgagg
      601 tgagggacta ctgggtttct cacgatgtga aatggctgag ccaacggcat ccaaaatgga
      661 aaaaacttcg tgggattggg atgactaaga ataccatcga taaaggcggt atcatcacgg
      721 aagaagtccg ttactttatc cttagtttca agggtgatgt gcagacattt tcacaggttg
      781 ttcgtggtca ttggtcggtt gaaagcctac attggctgtt agatatggtt taccgagaag
      841 ataagaacca aaccttagat aaacgagctg ctttcaatct gaacgctatt cgtaaagttt
      901 gtcttcatct cttacaaatg atgacattcc ctaaggagaa gttgagttac cgccgcaaac
      961 aacgctatat tt
//
LOCUS       DATUCG010000251          929 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.256, whole genome shotgun sequence.
ACCESSION   DATUCG010000251 DATUCG010000000
VERSION     DATUCG010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..929
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.256"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            102..688
                     /locus_tag="VCA20_002440"
                     /pseudo
     CDS             102..688
                     /locus_tag="VCA20_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941239.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            777..>929
                     /locus_tag="VCA20_002441"
     CDS             777..>929
                     /locus_tag="VCA20_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HEP1836139.1"
                     /translation="MSHLQYTAKSHHLQWSVRQLSQICHHFYHNYCPDSFKYRRNVAL
                     VKVSDES"
BASE COUNT          287 a          149 c          208 g          285 t
ORIGIN      
        1 ttgataaact tcaggaagtt attcaaagct tgtctacttc tcaactaaaa tcaattgtac
       61 atagagagtg gacttccgct atttttgatt tttattgagt attacttgtt gaaaatttat
      121 cggtaagtca gaaagagaat actctctacg agattgaaaa actctgcttg gcatcaggac
      181 aggtggtggg tctggttgga gcgaatggtt ctggaaaaac aagccttgct cgttatctgg
      241 tcggtttagt ggaagataag aagagtagga tttcttggca aggtcaaacc ttatctagtc
      301 gccagagatt ggaaaaaaca gcctttgtta tgcaggatgt tcgactccaa ctatttgcgg
      361 aaagtgttga gcgagaactt acttttggaa ggaaaaatag agctgtagat gaacatttag
      421 tagctcgttt tgctttatcg gatttgttgg agagacatcc ggtcagttta tcgggtggtg
      481 aacaacagcg tgtgatgata gtggccagct tactggcaga taaggaaatt ttcatttttg
      541 atgaaccgac gagcggcttg gatttgagac gaatgcaaca agttgcttct gtttagtaga
      601 cttaaaaatg aagaataagt tagttttgtt aatttctcac gatgaagagc tattgaatct
      661 ggtttgtgat aaaatagtag atattaaaac ccgtggtgct agttcatttc aaactacaat
      721 aaaaagccca aatggactat actgtaagtg acgaaacata caggaggtta gtccaaatga
      781 gccacttaca gtataccgct aaatctcatc atttgcaatg gagtgtacgc caattatcac
      841 aaatttgtca tcacttctac cacaactatt gcccggattc ctttaaatat cggcgaaatg
      901 ttgccttagt taaagtatca gatgaatca
//
LOCUS       DATUCG010000252          869 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.257, whole genome shotgun sequence.
ACCESSION   DATUCG010000252 DATUCG010000000
VERSION     DATUCG010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..869
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.257"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            81..>869
                     /gene="cysK"
                     /locus_tag="VCA20_002442"
     CDS             81..>869
                     /gene="cysK"
                     /locus_tag="VCA20_002442"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEP1836140.1"
                     /translation="MAIYQNITQLVGKTPVIKLNNIVPEGAAEVYVKLEAFNPGSSVK
                     DRIALAMIEDAEKAGTIKPGDTIVEPTSGNTGIGLAWVGAAKGYKVIIVMPETMSVER
                     RKIIQAYGAELVLTPGSEGMKGAIAKAKEIAEEKNGWVPFQFANPSNPKVHEDTTGQE
                     ILEDFGTTGLDAFVSGVGTGGTVSGVSHVLKTANPDIAIYAVEADESAVLSGEAPGPH
                     KIQGISAGFIPDTLDTSAYDGIIRVKSDDALATGRAIGGKEGFLV"
BASE COUNT          245 a          154 c          208 g          262 t
ORIGIN      
        1 tcggcgagat agttttttta tattatcctt ttttgacttt ctactatata atgtataata
       61 acattatttg gaggtgccat atggctattt atcaaaacat tactcaatta gttggaaaaa
      121 cacctgttat taaactgaat aatatcgttc cagagggggc agcggaagtc tatgtgaaat
      181 tagaggcctt taacccagga tcttctgtaa aagaccgtat cgccttggct atgattgaag
      241 atgctgagaa agctggtaca attaaacctg gagatactat tgttgagcca acaagtggta
      301 acactggtat cggtcttgct tgggttggag cagcaaaagg ttataaggtg attatcgtca
      361 tgcctgaaac catgagtgtt gagcgtcgta agattatcca agcttatgga gctgaacttg
      421 tcttgactcc tggtagcgaa gggatgaagg gggctatcgc caaagcaaaa gagattgctg
      481 aagaaaaaaa tggctgggtt cctttccagt ttgctaatcc atccaatcca aaagttcacg
      541 aagatacaac aggacaggaa attttggaag actttggcac aactggttta gatgcctttg
      601 tatctggcgt tggtactggt ggtaccgttt caggtgtttc gcatgttttg aagacagcaa
      661 atccagatat tgctatctat gctgtcgaag ctgatgaatc agctgtctta tctggtgaag
      721 cacctggtcc gcacaagatc caaggtattt cggcaggttt tattccagat actttggaca
      781 ctagcgccta tgatggcatt attcgtgtca aatccgatga tgccttagct acaggtcgtg
      841 ctatcggtgg aaaagaagga ttcttagtt
//
LOCUS       DATUCG010000253          860 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.258, whole genome shotgun sequence.
ACCESSION   DATUCG010000253 DATUCG010000000
VERSION     DATUCG010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..860
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.258"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            64..771
                     /locus_tag="VCA20_002443"
     CDS             64..771
                     /locus_tag="VCA20_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018367901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HEP1836141.1"
                     /translation="MSKYKKVYQDIKKKIEEQVWSTGQALPTENELMEVYSYSKDTIR
                     KALSLLEMDGYIQKKQGKSSIVIEHGLMKDQYLSEIKTAGELNKRSQHQIQTQLTSLY
                     IIQGQEDLMSTFEVDDKVDFYRVSRVRTIDGERLEYDISYFDRRVVPYLSKEIAESSI
                     YRYLEEELHLTISHSRREISFRFANEEEKQLMDLGDYDMVVVVTSITYLSNGQAFQYG
                     TISYRPDKVSFVSMAKR"
BASE COUNT          290 a          154 c          169 g          247 t
ORIGIN      
        1 atagatgtat tgcccttttc tagctctttt atgatacact aatgggtaga aagacaggaa
       61 actatgagta agtataaaaa agtttatcaa gatattaaga aaaaaatcga agagcaggtt
      121 tggtcaactg gacaggccct accgacagaa aatgaattaa tggaagtgta ttcctactcc
      181 aaggacacca ttcgcaaagc actatctctt cttgaaatgg atggttatat tcaaaagaaa
      241 caggggaaat cttctattgt tatcgaacat ggtctaatga aggaccaata tctttctgaa
      301 attaaaacag cgggcgaatt gaacaaaagg tcacagcacc aaattcagac ccagcttact
      361 tcgctttata ttattcaagg ccaagaagat ttaatgtcaa ctttcgaagt tgatgataag
      421 gtagatttct atcgagtgag tcgggtacgg acaattgacg gtgagcggtt ggaatatgac
      481 atttcctatt tcgaccgtag ggtcgttcca tatctcagta aagaaattgc cgaaagctct
      541 atttaccgct acttagaaga agaactccat ttaacaattt ctcattccag acgtgaaatc
      601 tccttccgtt ttgcaaacga agaagaaaaa caattgatgg acttaggaga ctatgacatg
      661 gtcgtcgtgg tcactagtat cacctatctt tcaaatggtc aagctttcca gtatggaaca
      721 atcagttacc gtccagacaa agttagcttt gtatcaatgg cgaaacggta aaaggaaact
      781 ttcgattttt tcgaccaagt gtataaaact tctagaattt tatagttata aaaagaatgt
      841 ctccgctttt ttgcagagac
//
LOCUS       DATUCG010000254          828 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.259, whole genome shotgun sequence.
ACCESSION   DATUCG010000254 DATUCG010000000
VERSION     DATUCG010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..828
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.259"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            1..634
                     /locus_tag="VCA20_002444"
                     /pseudo
     CDS             1..634
                     /locus_tag="VCA20_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027183.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT          274 a          142 c          192 g          220 t
ORIGIN      
        1 ctataccgag ggaagcagta attggacaca gaaacatgtc cgctggctcc attccctaaa
       61 tcctaaggga ctttacgcag agattttgac agaataccta ttgacctatg agaaattagt
      121 agatcaaata gaacggtatg atgcgcgaat tgagcaactg ggtcaaagcg acagttatca
      181 agagaaggtc tcacggctct cttgctttat tggcattaaa acactaactg ctctttccat
      241 tgtgacggaa atcggtgatt ttaaccgctt tgcgacagct caacattttg cttcttatct
      301 taggctaact cctagcgaaa attctagtgg cgacaaggag agaagaggtg ctatcaccaa
      361 agctggtaat agccatgtga gacgacttct gatagaagct gtacaatcat tggctaaggg
      421 gactattggg tataaatcca aagaattgaa aaggagataa agtggaaacc gagtggaggt
      481 gattgcttat gcggataagg ctaatgagcg cttaagaaga cgttatcgca cacttgttct
      541 aggaaaaaat aagaaacaaa atgttgttaa aacagctact gcacgagaat tatctggttt
      601 tattttgggg atgatgacag gaagaatagc ttgaagttag cgcttgtttt catgtacact
      661 tttgacaatt aagttttatc atcgcagagc gatgaggaat ccctatttct atcccaaatc
      721 actgggtgaa tttggacaga aataaggatt gaaataatgt ttgaatggag agtatagtca
      781 actgaaacaa gagcaggacg aaagtacctc aagaaacgta tcgcaatt
//
LOCUS       DATUCG010000255          821 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.260, whole genome shotgun sequence.
ACCESSION   DATUCG010000255 DATUCG010000000
VERSION     DATUCG010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..821
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.260"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            32..271
                     /locus_tag="VCA20_002445"
     CDS             32..271
                     /locus_tag="VCA20_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEP1836142.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFISERGKI
                     LPRRVTGTSAKNQRKVTAAIKRARVMALLPFVNED"
     gene            complement(664..821)
                     /locus_tag="VCA20_002446"
                     /pseudo
     CDS             complement(664..821)
                     /locus_tag="VCA20_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          268 a          156 c          182 g          215 t
ORIGIN      
        1 gtcaaaatta ataatataaa ggagaacaca catggctcaa caacgtcgtg gcggtttcaa
       61 acgccgtaaa aaagttgact atatcgcagc taacaaaatt gaatatgttg attacaaaga
      121 tactgagctt cttagccgtt tcatttctga acgtggaaaa atccttccac gccgtgtaac
      181 tggaacttca gctaaaaacc aacgtaaagt aacagcagct atcaaacgtg ctcgcgttat
      241 ggctctttta ccattcgtaa acgaagatta aaaaaatggt ccagtggacc attttttctt
      301 gcgcccaaga aactggaaag gcgcaacggc gagaatggac tgttttttca cgagctcgga
      361 aattagaaga cgagttgaga ccctgacggg tctttttagc ttgcacccaa gaaactggaa
      421 aggcgcaacg gcgagagtgg accatttttt catgaactcg aaattaagaa agcgaattaa
      481 gttcttgaat ggactgtttc aacccgagcc aagaagttag aaagcgagtc acagtctggg
      541 gatagactgt ttcagcagat tacatagaca agtgacagcg gataaagttc ttgggtgatt
      601 tgatagctga actgtataga taaacaaaag cgactggaat attccaatcg ctttgtttta
      661 tagtcaactg aaacaagagc agaacaaagt acctcaagaa acgtatcgca attcggcaat
      721 actcttctga ggtgtttttt gatatgagcc catattttct caatgggatt atattctggt
      781 gagtaaggag gaagtggtaa aagtctatgt ccctgctcct c
//
LOCUS       DATUCG010000256          820 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.261, whole genome shotgun sequence.
ACCESSION   DATUCG010000256 DATUCG010000000
VERSION     DATUCG010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..820
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.261"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(230..427)
                     /locus_tag="VCA20_002447"
     CDS             complement(230..427)
                     /locus_tag="VCA20_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1836143.1"
                     /translation="MKQPFMITGTTIARAVEIGSILLLAYILLSDLLGWSDMDTALMT
                     TLIASITCNEASLQMEKKGDK"
BASE COUNT          261 a          157 c          140 g          262 t
ORIGIN      
        1 tcgtcttaac cgctagaaaa tttgtgcgtc tggtggatgc gctactacgc aatcgccaac
       61 tctatacgcc accaaggagg cttatggaag ataagtgatt atcggcacaa gtccttgatt
      121 aagctagtga aaaaaagcgt ttttcgctag gtgtttttag tgcaccctgt tttctaaaaa
      181 atcaactaaa aattaatcaa ctttctattg actttttacc actagacttc tacttatctc
      241 cttttttctc catttgtaaa cttgcttcat tacaagtaat tgaagcaatt agagtagtca
      301 ttagagctgt atccatatcg ctccatccca gcaaatcaga aaggagaatg taagccaata
      361 atagaatgga ccctatttcg actgcacgcg caatggttgt tcctgtaatc ataaaaggtt
      421 gtttcatttg gtgactcctt atctgttcat cgtatcagat gaaccttttt gattttcatt
      481 tcaatatcat tgtaacactt ccttttcttt ttgacaacta tctttgtcaa aaaaataata
      541 atgtttgtca aaaagtttat gaacattgtc aaaatgtagt aaatattggt caggatagcc
      601 aaaattcata aaaaagaagc aagattactc tgtcaagcac cagataaaat ggacagttaa
      661 aaaagttagt tttttagaag gtatgcttcc ctaaattcta ggggagtcat aacttttaat
      721 tatgtgactt ttagtccgtc acgctccgta aggtgcgtga caaataaacc acccgctatg
      781 cgggtgcgcg acgaaagtta taccaaaaaa actccaaacg
//
LOCUS       DATUCG010000257          796 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.262, whole genome shotgun sequence.
ACCESSION   DATUCG010000257 DATUCG010000000
VERSION     DATUCG010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..796
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.262"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            5..714
                     /locus_tag="VCA20_002448"
                     /pseudo
     CDS             5..714
                     /locus_tag="VCA20_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027345.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
BASE COUNT          243 a          140 c          180 g          233 t
ORIGIN      
        1 actcatgaaa gccttaattt ttgatgtaga tgatacgctt tatgatcaga ttcaaccgtt
       61 tgaacgtgct ttggaaagac acattgaagt agcaaggagc aaattgaacc actctacctt
      121 tcctttcgca gatatgcgga tgaagtattt gaagcgacag caattggaaa gatgagtctg
      181 aaagacagtc atatttatcg gatgaaacat gctttggcag attttgggta tcaggtgtct
      241 gatgcaacag ccttagctat tcaaatagat tatgattatt ttcaagggca attggaattg
      301 agtccgattt ttccagagat ttttgcatat tgccaatcaa atggtattgc tttgggtatt
      361 atcaccaatg gtccccaccg gcatcaactg cgtaaaattc gtaccatgca gttgggtcaa
      421 tggattgagt ccgaccacat tatcatttct ggtcaggtcg gtgtgaccaa gccaaatcca
      481 aagattttca aattgctgga ggaacgttta gaattgcctg cgagcgacat cgtttatctg
      541 ggagattcct ttgaaaatga catcatcggg gcaaaaacag ctggttggca agcggtctgg
      601 tttaatcaca gaaaacggtc agagccagaa agttcctttc aggcagatta tatgattgac
      661 gaatggttca atctggattc ccttatcaaa aacatcataa agccgattgg ataaataaaa
      721 tggaataact ctcctaacaa gtttttaata ggattagaag ctagtcgggc gagtttttgt
      781 gttcaaaaag aggagg
//
LOCUS       DATUCG010000258          778 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.263, whole genome shotgun sequence.
ACCESSION   DATUCG010000258 DATUCG010000000
VERSION     DATUCG010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..778
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.263"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(70..778)
                     /locus_tag="VCA20_002449"
                     /pseudo
     CDS             complement(70..778)
                     /locus_tag="VCA20_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898371.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT          225 a          186 c          143 g          224 t
ORIGIN      
        1 gaccgcaggg tgacgagggg aatcacgtac cagattcatt tacaaaaaaa gaaaaaagtt
       61 gaaaatgttt caactttttt ctgttaaatc caccacatag gttaattctt gatagacatc
      121 ttctatgtca aagaaatcta ggcctgcttc ttcgctgact acataaacac cttctctcaa
      181 atagtaggga actggctgac cttccatatc tgaaaaggac aatctgcctc gctctaaatc
      241 aattccaaag cggaaggttg gaatatcctt gggtgctccg acgactagat agtcagtgca
      301 aaccaattgt ccaccccatt tcttatacaa atggttgctg cgtcatcctc acgagtaaag
      361 atggctaatt tctctacacc ttgctctctc aactcctgct ctgctttttc aaaaagcgcc
      421 tgggcaatgc cctgcccttg aaaatcaggg tggactgcta ggttagtaaa ataagctgtt
      481 cgcttacttg gtgtgtaaat ataagactgg ctgcactcct cagtgtaaat atcgatgtcg
      541 attagaccaa cttaatagca gaaacttgct acgcaagtcc catctccaac ctcaaacggt
      601 tccccaaacc gtttgagcca tctacttcag ccacccattc cacacgatga ttataaacgt
      661 ctgtcattag ttcgggcttc tctgtttctc tatcatcaaa gaatggagag aagaggtagc
      721 tgagtgcctt acagtaaacc cagcattttt catcactgat ttgatatttt ctgataat
//
LOCUS       DATUCG010000259          762 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.264, whole genome shotgun sequence.
ACCESSION   DATUCG010000259 DATUCG010000000
VERSION     DATUCG010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..762
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.264"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(71..721)
                     /locus_tag="VCA20_002450"
     CDS             complement(71..721)
                     /locus_tag="VCA20_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEP1836144.1"
                     /translation="MTEQNTILTQLEAASHAGTLKRYERIGETKPYYGVPMGAISGIA
                     KAYKNRLDLFAPLWQTGILEAQYLAIQVAHADLETCLNEVVSVNVLDKLASIILSKRK
                     DCKDWEETLLAQKPAIFQRIGWFLRAKYFTGKTATNQEIEETLDYIRQHLQTADPLVQ
                     WTMNQCLVEIAVTYPDYLEQGLVIGQELAVYSDMKVPKGCTSAYAPDWIEALLRRK"
BASE COUNT          197 a          156 c          175 g          234 t
ORIGIN      
        1 atcgcttgct tgaaaagggc gtaaaattag acgttctgtt tctaaaatct gagttccttt
       61 atgttgcatc ttatttcctc ctcaacaatg cctcaatcca gtcgggtgcg taggcagaag
      121 tacagccttt tggcaccttc atatcggaat agacagcgag ttcctgaccg attacaagtc
      181 cctgttccag atagtcagga taggtaactg caatctccac taagcattgg ttcatggtcc
      241 attggacaag cgggtcagcg gtttgcaaat gctggcgaat atagtctaga gtttcttcaa
      301 tttcttggtt tgttgctgtt tttccagtaa agtatttggc acggaggaac cagcctatgc
      361 gttgaaagat ggctggtttt tgggcaagca aggtttcttc ccaatcctta cagtcttttc
      421 gtttactgag gatgatagaa gccagtttgt ctagtacatt aacagaaact acttcattga
      481 ggcaagtttc caaatcagca tgggcaactt gaatagctag gtattgagct tctaaaatac
      541 ctgtttgcca gagtggagca aacaagtcca gtcgattttt ataggccttg gcgataccag
      601 aaatagctcc cattggaacg ccgtaatagg gcttagtttc gccaattctt tcatagcgct
      661 tgagggtgcc agcatgactg gctgcttcaa gttgggtgag aatggtgttt tgttcagtca
      721 taaatatctc cgaaaaaaac tggattgctc ccaatgtcat ta
//
LOCUS       DATUCG010000260          752 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.265, whole genome shotgun sequence.
ACCESSION   DATUCG010000260 DATUCG010000000
VERSION     DATUCG010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.265"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            118..687
                     /locus_tag="VCA20_002451"
     CDS             118..687
                     /locus_tag="VCA20_002451"
                     /inference="COORDINATES: protein motif:HMM:TIGR01168.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEP1836145.1"
                     /translation="MRHNGKNQMFPDKRQRFSIRKFKTGVASVVFVALFLGNMATVAA
                     EQVPTNTDSSAKEVMESDVALPTDETLDKVPTTDEEVAPIEEGVDASLAPLESAEAPQ
                     EFRAAVGTERSAATNTKGVVVTPGQDITTISEIPTDGFTTTEPEVKSMNPNFTGTVNH
                     DPKNYTFAVIKTDNLNKTGPNTNVIKLSI"
BASE COUNT          269 a          123 c          161 g          199 t
ORIGIN      
        1 taagtacaaa agggggatga tgtttctgtt ttgaaaagaa caatttgaag agacttatga
       61 atattgtttt cacgaccagc gacaaaaaat atctatattt atattaagga gaaaataatg
      121 agacacaatg gaaaaaatca aatgtttcca gataagcgtc aacgtttttc aattcgtaag
      181 tttaaaacag gagtagcatc cgttgtcttt gttgcactat tcttgggtaa catggctaca
      241 gttgctgcag agcaggttcc gacaaataca gattcatctg ctaaagaggt aatggaaagt
      301 gatgttgctt tgccaacgga tgagacattg gataaagtgc caacaactga tgaagaagta
      361 gcacctattg aagagggagt ggatgcctcg ttagcgccac tagagagcgc tgaggcacct
      421 caagaattta gagcagctgt aggaactgag cgttcagcgg cgacaaatac taaaggagta
      481 gttgtaacac caggacaaga tatcacaaca attagtgaaa ttccgacaga tggttttacg
      541 acaactgagc ctgaagtaaa gtcaatgaac cctaacttta ctggcacggt aaatcatgat
      601 cctaaaaatt atacatttgc tgttattaaa acggataatt taaacaaaac aggtccaaat
      661 accaatgtca ttaagttaag catttaagca gctaaagaaa ctattcccgt ctgagcaatt
      721 gatgacaaaa cgggaatagt ttttgtattt ag
//
LOCUS       DATUCG010000261          751 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.266, whole genome shotgun sequence.
ACCESSION   DATUCG010000261 DATUCG010000000
VERSION     DATUCG010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.266"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            <1..663
                     /locus_tag="VCA20_002452"
     CDS             <1..663
                     /locus_tag="VCA20_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HEP1836146.1"
                     /translation="RHFYSICHLFPCGQLLERSRFNRRSKQLIWLVQFIRKAMSEMLP
                     SDKLAIIDSFPLPLCQPVRNHRVTIFKGLADIGYNASKHLWFYGFKVHMLVTLSGFIL
                     NYVVTPASVHDIKVVYELLEGCKQSVILGDLGYLSSELKKDLKREGYPLWTPLRQNMA
                     GAEEHNDWKVMAMRRTIETRFSELCRLFDIEHTLARGLAGLQLRMEQIILAHNLRYFE
                     MN"
BASE COUNT          219 a          153 c          144 g          235 t
ORIGIN      
        1 cgtcatttct acagcatctg ccacctcttc ccttgcggtc agttactaga aagaagccgt
       61 ttcaatcgtc ggtcaaaaca attgatttgg ctagttcaat tcatccgaaa agctatgagt
      121 gaaatgcttc catctgataa actagccatt atagatagtt ttcctttgcc actttgccaa
      181 cccgttcgca atcacagagt taccattttt aaaggattag ccgatattgg ctacaacgcc
      241 tctaaacatc tttggttcta cggcttcaag gttcatatgc tagtgacctt atctggtttt
      301 attctgaact atgttgtcac acctgcctct gttcacgaca tcaaggtggt ttatgagcta
      361 ttagaggggt gtaagcaatc ggttatctta ggtgatttag gctacctaag cagtgaactc
      421 aaaaaggact tgaaacggga aggttaccct ctatggacgc cacttcgaca aaatatggca
      481 ggggctgaag aacataacga ttggaaagta atggccatga gacgaactat tgaaacacgt
      541 ttttctgaac tgtgtcgtct ttttgacatc gaacatacat tagctagagg tctagctgga
      601 ctacagttac gaatggaaca aattattcta gctcataact tacgctattt tgaaatgaac
      661 tagcaccacg ggtatattat atcacaaatg tactgttttt gtttcattta actatatttt
      721 aaataaaaaa cctcgtacta ttttgtacga g
//
LOCUS       DATUCG010000262          718 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.267, whole genome shotgun sequence.
ACCESSION   DATUCG010000262 DATUCG010000000
VERSION     DATUCG010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..718
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.267"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            76..717
                     /locus_tag="VCA20_002453"
                     /pseudo
     CDS             76..717
                     /locus_tag="VCA20_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994445.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
BASE COUNT          247 a          110 c          145 g          216 t
ORIGIN      
        1 ttattttgaa aacttgtaaa cgtttttccg agaattattt tacactaact aaaatagatg
       61 tttaggagga aaaacatgta taaagccatt atttttgata tggatggagt tatttttgat
      121 actgaaggtt tctattatca aagacgtaaa acctttttag ataataaagg gatttccatt
      181 gagcatatgg aagccaaaga atttattggt ggcaatctcc agcaagtttg gcaaaagatt
      241 ttaagtgaaa atgagcatgt tggttaagct gatacctttc atcaggagta tgaagattat
      301 aaagcaaagc accgtgcgcc ttattcccaa atcatttttc cagaagacag acaagcttta
      361 gctgatttaa aggcaacagg tctaaaacta gtagccttag cttcaaatac gcaaaagaat
      421 gatgatctct ttgctttaga atcagcatca attttgtcct attttgatat tgttcttagt
      481 cgagaagatg tggacagcca aaaaccgcaa ccagatattt atcttaaagc agctggatta
      541 ttggggatag ataaagctga tagtttaatt attgaagata gtcaaaaggg tatcagcgct
      601 ggcaagtctg ccggaatgca agtctgggca atcaaggata agcaatatgg gattgaccag
      661 tcgcaagctg atcaacttat cgaccatttg ggacaagtcc cagaaaaagt actttaaa
//
LOCUS       DATUCG010000263          715 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.268, whole genome shotgun sequence.
ACCESSION   DATUCG010000263 DATUCG010000000
VERSION     DATUCG010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..715
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.268"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            106..618
                     /locus_tag="VCA20_002454"
     CDS             106..618
                     /locus_tag="VCA20_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEP1836147.1"
                     /translation="MIVLITGASHTGKTVLAQKLLEKYHYPYLSIDHLKMGLIRSGNT
                     KLSVEEEDKLIAYLWPIVREMIKTAIKNEQNLIVEGIYIPFDWATDFATAYLDHIRYY
                     CLVMSENYIQKHFGSIKKHARLIENRIDDEWCTMESVLEENTRFFRRAKKYDANILWI
                     DESYEIEIDL"
BASE COUNT          265 a           98 c          139 g          213 t
ORIGIN      
        1 aataaattga gtaagagatt aaaaaataga taggatgaca agcagtgttt cttacaaatt
       61 ttcatataaa acatagatgc tttgtcgctt agataggaga aaatcatgat tgttttaata
      121 acgggagcat ctcacactgg gaagactgtg ctagctcaaa aattacttga aaaatatcac
      181 tatccttatt tatcaataga ccatttgaaa atggggttaa ttcgtagcgg caatactaaa
      241 ctttcagtcg aagaagagga taaacttata gcatatctat ggcctattgt tcgtgaaatg
      301 attaaaactg ctataaaaaa tgagcaaaac cttattgttg aagggatcta tatccctttt
      361 gattgggcaa ccgactttgc aacagcctac cttgaccata tcagatatta ctgtcttgtg
      421 atgagtgaaa actatattca gaaacacttt ggaagcataa agaaacatgc acgccttata
      481 gaaaatcgta tagatgatga atggtgtacg atggaaagtg ttttagaaga aaatactcga
      541 ttttttagaa gggcgaaaaa atatgatgca aacatcctat ggattgatga gagttacgag
      601 atagagattg atttatgata gaaagacatt gtatctcagt ttatctaagg gatataacta
      661 agaggaagta acaccagata ggcgttgctt cctttttatt tctaagaaat agaaa
//
LOCUS       DATUCG010000264          707 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.269, whole genome shotgun sequence.
ACCESSION   DATUCG010000264 DATUCG010000000
VERSION     DATUCG010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.269"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(131..571)
                     /locus_tag="VCA20_002455"
                     /pseudo
     CDS             complement(131..571)
                     /locus_tag="VCA20_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775050.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
     gene            complement(576..>707)
                     /locus_tag="VCA20_002456"
     CDS             complement(576..>707)
                     /locus_tag="VCA20_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEP1836148.1"
                     /translation="LPPYSPEYNPIEKTWAHIKKHLRKVLPNCDTFFEVLLSCSCFG"
BASE COUNT          229 a          135 c          156 g          187 t
ORIGIN      
        1 aagatttttg aaaaaataac ttgtgtttga ttgggatttg caagataata atgttggctt
       61 tgcatatcat tccatcattt cgtggttgtt ttgagtttat gatacgcaaa gtgtttttat
      121 ttagctagaa ttaactagca caacgggtgg atatttttaa aaaaatgaga ccataaagga
      181 cagcagcaag caagcctcca atgatcggac caactactgg tacctaagca taaccccaat
      241 ctgaatctcc cttgtgcttc aatggaagga tggcatgcat gatacgagga ccaagatcac
      301 gcgcagggtt tagggcatat cctgttggac caccaagaga aactccgagg gaaacaatca
      361 aaccaccgac gattaaagtg ccaatccctg ttggaaaatc atactgacca aaagccagta
      421 atcccaagac caaaacaaag gttccgattg cttcgctaat tgtattggaa acaaggttgc
      481 ggatggcagg tccagtagag aaagttgcca gtacagcacc agtgtcttct gttgcatcgt
      541 agtgatcttt gtacatgagg tagaccaaga tatagtcaac cgaaacaaga acaggacaaa
      601 agtacctcaa aaaacgtatc gcaattcggc aatacttttc tgaggtgttt tttgatatga
      661 gcccatgttt tctcgatagg attatactct ggtgagtaag gaggaag
//
LOCUS       DATUCG010000265          667 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.270, whole genome shotgun sequence.
ACCESSION   DATUCG010000265 DATUCG010000000
VERSION     DATUCG010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..667
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.270"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            1..667
                     /locus_tag="VCA20_002457"
                     /pseudo
     CDS             1..667
                     /locus_tag="VCA20_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027183.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT          197 a          142 c          151 g          177 t
ORIGIN      
        1 tgcagaagtg ttatgtggct acgaagcccg gatgtcttgg atttacccta tatcaccagc
       61 tacaagctca cgggcttccc tgtatcgtga tggcgcctac aacggtgatg aaggaaggat
      121 ctaagcgtgt taagactgat aaaaaagatg cagctcagct cgcaaaagct ctggcctttc
      181 gtagctatcg gcctgttcat attcctactg ttgaggatga acaagtcaaa gaatatatcc
      241 gcatgagaac agaccacaaa gtggctctga agaaaatcaa acaacaaatt cttgccttct
      301 gtctccgaca tgattttctc tataccgagg gaagcagtaa ttggacacag aaacatgttc
      361 gctggctccg ttccctaaat cctgatggac tttatgcaga gattttgaca gaatatttat
      421 tgacctatga gacattagta gatcaaatag aacggtatga tgcgcgaatt gagcaactgg
      481 gtcaaagcga cagttaccaa gagaaggtct cacggctttc ttgctttatt ggcattaaaa
      541 cactaaccgc actttccatt gtgacggaaa tcggtgattt taatcgcttt gcgacagctc
      601 aacattttgc ttcttatatt gggctaactc ctagcgaaaa ttctagcggc gacaaggaga
      661 gaagagg
//
LOCUS       DATUCG010000266          650 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.271, whole genome shotgun sequence.
ACCESSION   DATUCG010000266 DATUCG010000000
VERSION     DATUCG010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.271"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            51..374
                     /locus_tag="VCA20_002458"
     CDS             51..374
                     /locus_tag="VCA20_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1836149.1"
                     /translation="MNRDKKPGMETVKIGGIVYEVSKEPDLQGKSGEWGHIEYKTGKI
                     VLDDSASQQIEDQTLIHEIAHGMLVEAGYVQHEEEQADRIGKILYQVLTDNDFSWLWK
                     GGTNG"
     gene            367..546
                     /locus_tag="VCA20_002459"
     CDS             367..546
                     /locus_tag="VCA20_002459"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1836150.1"
                     /translation="MASFSIDLALNWENQDELQRLLQNVDKAQQAYQNALKELSEFKP
                     DIQVVSKNGGYKAHE"
BASE COUNT          231 a          108 c          163 g          148 t
ORIGIN      
        1 acctagagaa atctaagtgc tttttttgta ccaaaaaaac aggaggaaac atgaatagag
       61 acaagaaacc aggtatggaa actgtcaaaa ttggcggtat agtttatgaa gtcagcaagg
      121 aacctgattt acaggggaag tctggtgaat gggggcatat tgagtacaag acaggtaaga
      181 ttgtgcttga tgactctgcc agccagcaga ttgaggacca gacacttatc cacgagattg
      241 ctcatgggat gttagtcgaa gctggttacg tgcagcatga ggaagaacaa gcggaccgca
      301 ttggcaaaat tttgtatcaa gtgctgaccg acaatgattt cagttggttg tggaaaggag
      361 gaaccaatgg ctagtttttc aattgacctg gcgctaaact gggagaacca agacgaactt
      421 caacgtttgc tgcaaaatgt cgataaagcc caacaagcgt accagaatgc tttgaaagaa
      481 ttgtctgagt tcaaaccaga cattcaggtt gtttctaaaa atggaggtta caaggcacat
      541 gaataaacgt atcaaaaaga aacgtgaact gattgaacaa gttcagggaa ctaaagaagc
      601 tgttgatata gtaaactgaa acaagagcag gacgaaagta cctcaagaaa
//
LOCUS       DATUCG010000267          612 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.273, whole genome shotgun sequence.
ACCESSION   DATUCG010000267 DATUCG010000000
VERSION     DATUCG010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.273"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            1..72
                     /locus_tag="VCA20_002460"
     tRNA            1..72
                     /locus_tag="VCA20_002460"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33..35,aa:Asp,seq:gtc)
     gene            138..210
                     /locus_tag="VCA20_002461"
     tRNA            138..210
                     /locus_tag="VCA20_002461"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:171..173,aa:Lys,seq:ttt)
     gene            214..295
                     /locus_tag="VCA20_002462"
     tRNA            214..295
                     /locus_tag="VCA20_002462"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:248..250,aa:Leu,seq:tag)
     gene            309..381
                     /locus_tag="VCA20_002463"
     tRNA            309..381
                     /locus_tag="VCA20_002463"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:342..344,aa:Thr,seq:tgt)
     gene            394..465
                     /locus_tag="VCA20_002464"
     tRNA            394..465
                     /locus_tag="VCA20_002464"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:426..428,aa:Gly,seq:gcc)
     gene            473..556
                     /locus_tag="VCA20_002465"
     tRNA            473..556
                     /locus_tag="VCA20_002465"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:507..509,aa:Leu,seq:taa)
BASE COUNT          144 a          135 c          182 g          151 t
ORIGIN      
        1 gtcccgtagt gtagcggtta tcacgtcgcc ctgtcacggc gaagatcgcg ggttcgattc
       61 ccgtcgggac cgttcaaata gtctgggaga ctatttgaag ttggaaatag agcaaacaga
      121 gtttgcgtca tatttacgac tcgttagctc agttggtaga gcaattgact tttaatcaat
      181 gggtcgctgg ttcgagccca gcacgagtca tatgcgggtt tggcggaatt ggcagacgca
      241 ccagatttag gatctggcgc tttcgggcgt gggggttcaa gtcccttaac ccgcatagaa
      301 tagaataggc cggcttagct cagttggtag agcatctgat ttgtaatcag agggtcgcgt
      361 gttcaagtca tgtagccggc attaggaaag attgcgaacg tagttcagag gtagaacacc
      421 accttgccaa ggtgggggtc gcgggttcga atcccgtcgt tcgctttgag aggccggggt
      481 ggcggaactg gcagacgcac aggacttaaa atcctgcgat ggcaacatcg taccggttcg
      541 attccggtcc tcggcatgaa gattataata gttagcaccc ttagctcaac tggatagagt
      601 acctgactac ga
//
LOCUS       DATUCG010000268          597 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.274, whole genome shotgun sequence.
ACCESSION   DATUCG010000268 DATUCG010000000
VERSION     DATUCG010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.274"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
BASE COUNT          165 a          113 c          119 g          200 t
ORIGIN      
        1 cgagtaggct ggttttttga tgtgtaaatt ggactacaat ctttattaaa tatgctataa
       61 tagtttttgc agaaaagtaa agacggtggc tctaatttct gaaaggtagg tggtgcctat
      121 gggcaaatca tcaaaatcta acagaaaggc tagcccaagc cagctggtaa gtgaacttac
      181 tcatctggca acgacaaatc ctataagatt tgtctagggc tcacactagc tcgagttttg
      241 aaaaatatcg tttcaaaact ctcacgtcga aggctttagc ttgtcaacct ttgagtggct
      301 aacactcatg tttgtagctg gtacagtcat ccagtttttc ttttattact tcgttaactc
      361 gctttgccgt actcaagtac tgtctgcagc tcgttgccta gtactaaaag taaactgggt
      421 gactattatc ttttaccgct ctcgtgatgt tagtgcttga attggtaaaa tcgacaaaaa
      481 aataaccgtc tataactttg gcgagttcac ggttattttt taaataattt gtaaacagcc
      541 accgtcttaa acggttctgc attgggtagt tgtttctggc aactaccttt ttctatg
//
LOCUS       DATUCG010000269          588 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.275, whole genome shotgun sequence.
ACCESSION   DATUCG010000269 DATUCG010000000
VERSION     DATUCG010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.275"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            <1..142
                     /locus_tag="VCA20_002466"
     CDS             <1..142
                     /locus_tag="VCA20_002466"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312208.1"
                     /note="catalyzes the formation of
                     (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-
                     carboxamido)succinate from
                     5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate and
                     L-aspartate in purine biosynthesis; SAICAR synthase;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEP1836151.1"
                     /translation="ADEFSPDNCRLWDADGKHLDKDVFRRGLGELTEVYEVVLVKLQE
                     VK"
     gene            155..>588
                     /locus_tag="VCA20_002467"
     CDS             155..>588
                     /locus_tag="VCA20_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEP1836152.1"
                     /translation="MAKRIFVEKKADFQIKAEALRKELTHNLQLTSLTSVRLVQVYDV
                     FHLEDDLLEQAIKHIFTEQVTDKVLSEAELDLEEAAYFAIEALPGQFDQRAASSQEAL
                     LLLSSRQEALLLLGSRQEVRVNTGHLYILNGDVQEEELATIK"
BASE COUNT          141 a          102 c          180 g          165 t
ORIGIN      
        1 ggcggatgag ttttctcctg ataattgtcg tttgtgggat gcggatggca agcatctcga
       61 caaggatgtt ttccgtcggg gtctcgggga gttgaccgag gtttacgagg tcgtattggt
      121 aaaattacaa gaagtgaagt aaggattgga aaaaatggcg aagcggattt ttgttgagaa
      181 gaaggcggat tttcagatta aggcggaggc tcttcgcaag gagttgactc ataatttaca
      241 gttgacaagt ttgacgagtg tacgtctggt gcaggtttat gatgtcttcc atctggagga
      301 tgacttgctg gagcaagcta ttaagcatat ctttaccgag caggtgacag acaaggtctt
      361 gtcggaagct gagcttgatt tggaggaggc tgcttatttt gcgattgagg ctcttcctgg
      421 gcagtttgac cagcgggctg ccagcagtca ggaggccctt ctcttattga gcagtcgtca
      481 ggaggccctt ctcttattgg gcagtcgtca ggaggtgcgt gtcaatacag gtcacctcta
      541 tattttgaac ggtgatgtgc aggaagaaga attagctacc atcaagaa
//
LOCUS       DATUCG010000270          580 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.276, whole genome shotgun sequence.
ACCESSION   DATUCG010000270 DATUCG010000000
VERSION     DATUCG010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.276"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(84..233)
                     /locus_tag="VCA20_002468"
                     /pseudo
     CDS             complement(84..233)
                     /locus_tag="VCA20_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775244.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            complement(231..314)
                     /locus_tag="VCA20_002469"
                     /pseudo
     CDS             complement(231..314)
                     /locus_tag="VCA20_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000892893.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(319..>580)
                     /locus_tag="VCA20_002470"
     CDS             complement(319..>580)
                     /locus_tag="VCA20_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017284794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEP1836153.1"
                     /translation="NPYLDIDYFAQRIFNHGMKRLTGRFKLVWKKQDGRCHHCGLPME
                     LGEDREIFFKVPKSKGGMEEVDNMAYVHSYCQRLFIESRSKE"
BASE COUNT          171 a          132 c           99 g          178 t
ORIGIN      
        1 tacagcggaa gcctgaaaaa tcttgcacaa tcatttcaac tttacctact gagttgaaaa
       61 gaccatcttt cggtggcttg tttctaccac tttatcgata aattcgatag gtaatctgag
      121 gcaaattgag tcaagtgatt gcgactgtct ttggattcat aaaaatcaat catggcaaaa
      181 ccagctcgta attacccagc aatctgttct tctaaggtgt ggctaaattc atagtcgagg
      241 aggttagtgg tattactacc accttcctct ctaagaaccg tacgtgagag tttcccctca
      301 tacggctcaa gcatctcatc actctttcga gcggctctca ataaatagtc gctgacagta
      361 gctatgcaca taagccatat tatcaacttc ttccatacct ccctttgatt ttggaacttt
      421 aaagaaaatt tctctatctt ctccaagttc cattgggaga ccacaatgat gacagcgtcc
      481 gtcttgtttc ttccaaacaa gtttaaaacg acctgtcaac cgtttcatac catgattaaa
      541 gattcgttga gcaaaatagt ctatatccaa gtaaggatta
//
LOCUS       DATUCG010000271          564 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.277, whole genome shotgun sequence.
ACCESSION   DATUCG010000271 DATUCG010000000
VERSION     DATUCG010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..564
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.277"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            <1..>564
                     /locus_tag="VCA20_002471"
     CDS             <1..>564
                     /locus_tag="VCA20_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000512610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HEP1836154.1"
                     /translation="IRNGYVLVAENKPFNAQRHDVFIWGKRGYSSGEGGHTGIFVDNV
                     NIIHCNFKRNGITIDDYNKVSRGMYYYLYRPANQPSTSNKSLDQLVKETLAGVHGNGD
                     ARKASLGNQYEPVMAVINGKATASEKTDEELAREVLAGKHGAGEDRKRSLGPRYEPVQ
                     AIVNKLLKAKEKTSETVKNEPQTVQLED"
BASE COUNT          179 a          102 c          140 g          143 t
ORIGIN      
        1 atacgtaacg gatatgtttt ggttgctgaa aataaaccat ttaacgctca aagacatgat
       61 gtttttattt ggggcaaacg tggttattcc agcggtgaag gtggacacac tgggatattt
      121 gtagataatg tcaacattat ccattgtaac tttaagcgca atggtattac tattgatgat
      181 tacaataaag tatctcgtgg tatgtattac tatctatatc gcccagcaaa tcagcccagc
      241 acctccaaca aatcattgga tcagcttgtt aaggagactt tggctggggt acatggtaac
      301 ggagatgccc gcaaagcaag tttgggcaat caatatgagc ctgtcatggc agttattaat
      361 ggcaaagcta cggcaagtga gaagactgat gaggagcttg ctagggaggt cttagcaggt
      421 aagcatgggg ctggagagga ccgaaaacgg tcactaggac cacgctatga gcctgttcaa
      481 gccatagtca ataaattgct caaagctaag gaaaaaacgt ctgagacggt caaaaatgaa
      541 ccacagacgg ttcaattaga ggac
//
LOCUS       DATUCG010000272          561 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.278, whole genome shotgun sequence.
ACCESSION   DATUCG010000272 DATUCG010000000
VERSION     DATUCG010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.278"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            <1..>561
                     /locus_tag="VCA20_002472"
     CDS             <1..>561
                     /locus_tag="VCA20_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEP1836155.1"
                     /translation="QEEVYQNLRDLSRFYQNLTEDIVRTKNRLHKVLQVTFPEVENIL
                     STPTGEQYWNLVIAFPCKDFVLDLSKVELSESIRQSTSKRISDKRVAYLAEKLTALAN
                     QSYCAVKKTSPMLEEVRYYAKELLRLSEQRQTVLDQMVELAQPLPEYDILLSIPGIAE
                     TTATSIIGELGDIRRFQSANQINAFI"
BASE COUNT          167 a          132 c          113 g          149 t
ORIGIN      
        1 tccaagaaga agtctaccaa aacttgcggg atctcagccg tttctatcag aatctgactg
       61 aagatattgt tcgaactaag aaccgtctgc acaaggtctt acaagtcact tttcctgaag
      121 tggaaaacat cttgtcaaca ccaactggtg aacaatactg gaacctagtc atagcttttc
      181 cttgcaagga cttcgtgctt gatttaagca aggtcgaact ctcagagagc attcgtcagt
      241 ccacctcaaa acggatttcg gacaagcgtg tagcgtactt agctgagaag cttacagcac
      301 tagctaatca atcttactgt gccgtcaaga aaacctctcc aatgctggaa gaggtccgtt
      361 actatgcaaa agaattgctt cggctttctg aacagagaca aactgtccta gaccaaatgg
      421 tagaactagc tcagccatta cctgaatatg acattctgct ctctattcct ggaatagctg
      481 agactactgc aacaagtatc attggtgaac tgggagatat tcgccgtttt cagtcggcca
      541 atcaaatcaa tgcctttatc g
//
LOCUS       DATUCG010000273          549 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.279, whole genome shotgun sequence.
ACCESSION   DATUCG010000273 DATUCG010000000
VERSION     DATUCG010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.279"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            <1..421
                     /locus_tag="VCA20_002473"
     CDS             <1..421
                     /locus_tag="VCA20_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HEP1836156.1"
                     /translation="RDYWISHDVRWLSQRHPKWKKLRGIGMTKNTIDKDGIITEEVRY
                     FILSFKGDVQTFSQVVRGHWSVESLHWLLDVVYREDKNQTLDKRAAFNLNAIRKACLH
                     LLQDMTFPKEKLSYRRKQRYISVHLEDYLPQLFGHRG"
BASE COUNT          165 a           85 c          133 g          166 t
ORIGIN      
        1 gagggactac tggatttctc acgatgtgag atggttgagt caacgacatc caaaatggaa
       61 aaaacttcgt gggattggga tgactaagaa taccattgat aaagacggta tcatcacgga
      121 agaagttcga tactttatcc ttagtttcaa gggcgatgtg caaacttttt ctcaggttgt
      181 tcgtggtcat tggtcggttg agagcctaca ttggttgtta gatgtggttt accgagaaga
      241 taagaaccaa acattagata aacgagctgc tttcaattta aatgctattc gtaaggcttg
      301 tcttcatctt ttacaagata tgacattccc taaggagaag ttgagttacc ggcgtaaaca
      361 acgctatatt tctgttcatt tggaggatta tttgccacaa ttatttggac atcgaggata
      421 aggtggttgt aaaaaggtag ataccaagtt taaagcggtt atttccgggg acaaaaggag
      481 agaagaaatt tttcatgcgt atggcgtgat ttccacgtcc ttgtattgac ataggtgacg
      541 gaaaatatc
//
LOCUS       DATUCG010000274          540 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.280, whole genome shotgun sequence.
ACCESSION   DATUCG010000274 DATUCG010000000
VERSION     DATUCG010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.280"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            1..489
                     /locus_tag="VCA20_002474"
                     /pseudo
     CDS             1..489
                     /locus_tag="VCA20_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735942.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
BASE COUNT          168 a           87 c          130 g          155 t
ORIGIN      
        1 attgttatta taggcgcctc ctttgctggt ttaagcgcag ctttggaatg tcgaaaattg
       61 tatcaaaatg ctcaaataac actaattgat agagaaaaag atgtggctta ctttccaaat
      121 accttgaatt ggaaagtggc aggaaaaatt tctggttggg aagaagcgaa gatttctttg
      181 ttcagtgagg tgcagcatgc agctatttct tgtctttttg aaacagaatg tctcgcagtt
      241 tatcccgaaa tgcgaaaggt aaagatacgg catcatcaag atataggtga tattatttat
      301 gaccggctaa tattggctat gggagctagt caaacatggg aatggggaac ggaagaactt
      361 caagactatc taattcgctc aaaaacgcta tctcaagcac aagccagtct agagaaattg
      421 acagatgcaa aaacagttgc agtgattgga gcaggacaga ttggtttgga aagcttggat
      481 gcccttagct agttggattt gaatttgcat gtgtttgagg cgcaagattc actccttgca
//
LOCUS       DATUCG010000275          536 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.281, whole genome shotgun sequence.
ACCESSION   DATUCG010000275 DATUCG010000000
VERSION     DATUCG010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.281"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            <1..235
                     /locus_tag="VCA20_002475"
     CDS             <1..235
                     /locus_tag="VCA20_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1836157.1"
                     /translation="KEYYRSLNFYLMILLFLGSIGEWLNKGPSLWLALLLFATVGTAR
                     SAFWSKEWTLKANWQIYLCIAFLLVGAWIEFFV"
     gene            321..536
                     /locus_tag="VCA20_002476"
                     /pseudo
     CDS             321..536
                     /locus_tag="VCA20_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002278791.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          162 a           94 c          121 g          159 t
ORIGIN      
        1 taaagaatat tatcggagtt tgaactttta tttgatgata ttgttgtttc taggaagtat
       61 cggcgagtgg ctaaataagg gacctagcct ctggcttgca ctcttgctct ttgctactgt
      121 aggtactgcc cgtagtgcct tttggtctaa agaatggacc ttgaaagcaa attggcaaat
      181 ctatctctgt atcgccttct tgcttgttgg agcttggatt gaattttttg tttaagagtt
      241 gagggcgggg tgaaaagaac cgcttataat gaacagtata aaaaagttat atactgttca
      301 tataaggagg ttcttttttc atggctaaaa aaggaagcaa gttcaccaaa tatccacccg
      361 agttcaaact acaggtagtt gaagactacc tcagcggaaa gagcggaggg atgccttcta
      421 ttgtcaaaaa ataggggtta aaatcagaca accaagtttt aacttggaca agaaaatacc
      481 gagaagaccc tactttaccc tcccaagatt tacgaggtag aaagtccact ggtcgt
//
LOCUS       DATUCG010000276          526 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.282, whole genome shotgun sequence.
ACCESSION   DATUCG010000276 DATUCG010000000
VERSION     DATUCG010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.282"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            <1..>526
                     /locus_tag="VCA20_002477"
     CDS             <1..>526
                     /locus_tag="VCA20_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEP1836158.1"
                     /translation="ITEDGTVIKTPVNDETNAKPDSPYDTTDNKPTEIVTEDGSRYVL
                     IPSKTIGTENGTVEGGKTTEITYVYKKVANWVPEIPGVPENERPVIPYPFDPNNPDVP
                     ITPTPDTVIPNVPGYTPVDPKDNTPLKPVDPNDPGKGYVPPTPEKPGEDTLIPYVQNG
                     NVVVNYVTEDGTVI"
BASE COUNT          195 a          118 c           96 g          117 t
ORIGIN      
        1 acattacaga agatggtaca gtcatcaaga cacctgttaa tgacgaaaca aacgcaaaac
       61 cagatagtcc atacgacaca actgataaca aaccaacaga aatcgttaca gaagatggtt
      121 cacgttatgt cttgattccg tctaagacaa ttggtacaga aaatggtaca gttgaaggtg
      181 gcaagactac tgaaatcact tatgtgtata agaaagttgc gaactgggtt ccagaaattc
      241 caggtgttcc agaaaacgaa cgtccagtaa ttccatatcc atttgatcca aacaaccctg
      301 acgtaccaat aacaccaact ccagatacgg tgattccaaa cgttccaggc tacacaccag
      361 tagatcctaa ggacaacaca ccactgaaac cagttgatcc aaatgatcca ggtaaaggtt
      421 atgtgccacc aacaccagaa aaaccaggtg aagacacact aattccttat gttcaaaacg
      481 gtaacgttgt tgttaactat gtcacagaag atggtacagt tatcaa
//
LOCUS       DATUCG010000277          519 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.283, whole genome shotgun sequence.
ACCESSION   DATUCG010000277 DATUCG010000000
VERSION     DATUCG010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.283"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
BASE COUNT          173 a          101 c           82 g          163 t
ORIGIN      
        1 atttgaatta ggaaaaggac agttttaagt tatagtgtta ataacccact cctaaaataa
       61 ctttagagtt ctcaaattga caaacttagg tttgtcctgt ttctaattca ctcgactata
      121 aaactaccaa agcctagaat agtttgcaca acttcaaaag ctgtcaaaag atgctactcc
      181 tttccgtcag attttgatga attgcccata ggcatcacct cactttcaga aaatcaagag
      241 ccaccgtctt cacttttcta cccatctagt atagcatact cattcctaaa aattattgga
      301 atagtgcagt attcccgaaa tctttgagaa accataaaaa aattacaact cttctatagt
      361 gatgatattt tttcatttta tactcaatga aaatcaaaat cagactagct ccaaaggttt
      421 ggggaacctt tggaggttgg agatagagcg aacgtagttc gtttctactc agcagataga
      481 accctgttac tattttgttt caaggtaaca ggctgaaag
//
LOCUS       DATUCG010000278          502 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.284, whole genome shotgun sequence.
ACCESSION   DATUCG010000278 DATUCG010000000
VERSION     DATUCG010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.284"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            <1..>502
                     /locus_tag="VCA20_002478"
     CDS             <1..>502
                     /locus_tag="VCA20_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017284794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEP1836159.1"
                     /translation="ATQEIALEAKELIREFLLGRGLELSEEKTLVTHINDGFDLLGWN
                     FRKYKGKLIVKPSKNSIQAVIGKFSETILKRGKAWEQEVLIMKLNQQIGGWTNYHQSV
                     CASEAFSYLDYHLYELLWRWAKRRHPKKGQWWVSTKYWHRRGNRSWVFASGDKELIRV
                     DHTAIVR"
BASE COUNT          164 a           89 c          120 g          129 t
ORIGIN      
        1 gcgacacaag aaattgcatt agaggctaaa gaattgatta gagagtttct gcttggacga
       61 ggtttagagc tgtcagagga aaagacattg gtaactcata tcaacgatgg tttcgaccta
      121 cttggttgga attttcggaa gtacaagggg aaattgattg taaaaccatc taagaattct
      181 attcaagctg ttattggtaa attctcagaa accatcctca agcgaggaaa agcatgggaa
      241 caagaagtct taatcatgaa actaaatcaa cagataggag gatggacaaa ctaccaccaa
      301 tccgtgtgtg ctagtgaagc cttctcctat ctggactatc atttatacga attattgtgg
      361 cgatgggcaa aacgtcgtca tccaaagaaa ggccagtggt gggtttcgac caagtattgg
      421 catagaagag gcaatcgaag ttgggtattc gcttcaggcg ataaagaact cattcgagtt
      481 gaccatactg ctatcgtcag ac
//
LOCUS       DATUCG010000279          492 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.285, whole genome shotgun sequence.
ACCESSION   DATUCG010000279 DATUCG010000000
VERSION     DATUCG010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.285"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            79..459
                     /locus_tag="VCA20_002479"
                     /pseudo
     CDS             79..459
                     /locus_tag="VCA20_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636705.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
BASE COUNT          168 a          102 c           90 g          132 t
ORIGIN      
        1 ataaatcaaa ataaagagca taacaccttt tcattcagtg ttgtgccttg agtgaaacga
       61 aaggaataaa ttataaccat gactactact attatcggct tcccacgtat cggagaacac
      121 cgtgagttaa aatttatcac tgaaaaatac tttagaaatg aaattccaca agaagaactt
      181 ttggaagcag caaaggacct acgtgctaag cactggaata ttgttaaaga agcaggcatt
      241 actgaaatcc caagcagcga cttctcccac tatgacaatg ttttggatgc aactgttctc
      301 ttcaacatcg tacctaaagc agtacagaac cttgacttga ctgaccttga aaaatacttc
      361 gcttttgcac gtggttatca aggagaaaaa ggggacgttc gtgctagacc gatgaaaaaa
      421 tggttcaaca ccaactacca ctatatcgtt ccagctattg gacttgttcg gtaactacat
      481 catctcatgg tt
//
LOCUS       DATUCG010000280          490 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.286, whole genome shotgun sequence.
ACCESSION   DATUCG010000280 DATUCG010000000
VERSION     DATUCG010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.286"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
BASE COUNT          151 a           85 c          109 g          145 t
ORIGIN      
        1 ttttagttta cttttagtac gaggcaatga gccgcaggca taactgaagt taggcaaggc
       61 gagttcaaac aatccagtgg agtgtttgaa gttggaaata gggagtggga aagaactcga
      121 ctggtctaaa aagagttcgt ctttccccct gcacagttga ttaggtcaga tttggagtgt
      181 gaaacacgaa caaatctgcc aatcaaccac tgcgctgaga tgttgacacg aactctgaga
      241 agcgaggatg gactttctac ccagcctcct tacgtcgaag taataaaaga aaaactaaaa
      301 gacgatattt tataatttca cacagaggta gtctttggta agggttatct tacatattgt
      361 ctataactaa acattggcaa gtcatcatct tgatttacca attcattcac ttgagcttaa
      421 agaagacttt gtgcttttat tgtgtgagaa tgtgtcatct cttcaaagtt ttgcttgatg
      481 tagtacaagg
//
LOCUS       DATUCG010000281          485 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.287, whole genome shotgun sequence.
ACCESSION   DATUCG010000281 DATUCG010000000
VERSION     DATUCG010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.287"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            <1..>485
                     /locus_tag="VCA20_002480"
     CDS             <1..>485
                     /locus_tag="VCA20_002480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1836160.1"
                     /translation="QDESGKAVASTVKSVETKTTDKTAIATVTETATAPAGYELVAND
                     MTTSTTGVVEGANTKVVFKVVKKVAADTASTTTSSSTTDEPAATPATTSTVAEPTTVA
                     EAKVILEQNISEAQVLSDEANRLYTASAEGNEALKITADATKLAATKATAVFENSVAT
                     L"
BASE COUNT          182 a          105 c           98 g          100 t
ORIGIN      
        1 tcaagatgaa tctggaaaag ctgtagcatc tactgttaaa tcagttgaaa caaaaacaac
       61 tgataaaact gcgattgcaa ctgttactga aacagcaaca gcacctgcag gttatgaact
      121 tgtcgcaaac gacatgacaa cgtctactac aggagttgta gaaggcgcta atacgaaagt
      181 tgtcttcaaa gttgtgaaaa aagtagcagc agatacagca agtacaacaa catcatctag
      241 tacaacagat gaaccagcag caacacctgc taccacttcg actgtagcag aaccgacaac
      301 tgtagcagaa gcaaaagtca tccttgagca aaacatttca gaagctcaag tcttgtcaga
      361 tgaggcaaac cgactttaca cggcttcagc agaaggcaat gaagccttga aaataacagc
      421 agatgctaca aaactggctg caacgaaagc tacagcagtt tttgaaaatt cagtagcgac
      481 acttg
//
LOCUS       DATUCG010000282          483 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.288, whole genome shotgun sequence.
ACCESSION   DATUCG010000282 DATUCG010000000
VERSION     DATUCG010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.288"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            <1..324
                     /gene="tnpA"
                     /locus_tag="VCA20_002481"
     CDS             <1..324
                     /gene="tnpA"
                     /locus_tag="VCA20_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HEP1836161.1"
                     /translation="EIIEGHMMPDHVHMLVLISPKLSISDFMGYLKSKSALMIFDKHA
                     NLKYKYGNRKFWARGYYVSTVGLNEKTVAKYIREQEKNDIALDKLSVKEYEDSPVVLV
                     HFKIA"
     gene            complement(301..>483)
                     /locus_tag="VCA20_002482"
     CDS             complement(301..>483)
                     /locus_tag="VCA20_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEP1836162.1"
                     /translation="GAEEHNDWKVMALRRTIETRFSELCRLFDIEHTLTRGLAGLQLR
                     IEQIILAHNLRYFEMN"
BASE COUNT          157 a           81 c           99 g          146 t
ORIGIN      
        1 gaaatcattg agggacacat gatgccagac catgttcata tgcttgtctt gatatcccca
       61 aaactttcga tttccgattt tatgggatat ttgaaaagta aaagtgctct catgatattt
      121 gataaacatg ctaatttaaa atataagtat ggaaatcgaa agttttgggc tagaggctac
      181 tatgtgagta ccgttggact gaacgaaaag acggtagcga aatatattcg tgagcaggag
      241 aaaaatgaca tcgcacttga taagttaagt gttaaagaat atgaagattc acccgtggtg
      301 ctagttcatt tcaaaatagc gtaagttatg agctagaata atttgttcaa ttcgtaactg
      361 tagtccagct agacctctag ttaatgtatg ttcgatgtca aagagacgac acagttcaga
      421 aaaacgtgtt tcaatagttc gtctcaaggc catcactttc caatcgttat gttcttcagc
      481 ccc
//
LOCUS       DATUCG010000283          479 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.289, whole genome shotgun sequence.
ACCESSION   DATUCG010000283 DATUCG010000000
VERSION     DATUCG010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.289"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            <1..180
                     /locus_tag="VCA20_002483"
     CDS             <1..180
                     /locus_tag="VCA20_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEP1836163.1"
                     /translation="LKYLCAEQGHRLLPLPAYSPEYNPIEKTWAHIKKHLRRVLPNCD
                     TFLEVLLSCSCFSWL"
     gene            complement(178..479)
                     /locus_tag="VCA20_002484"
                     /pseudo
     CDS             complement(178..479)
                     /locus_tag="VCA20_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646735.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT          131 a          104 c           94 g          150 t
ORIGIN      
        1 ctaaaatatt tatgcgcgga gcagggacat agactgttac cccttcctgc ttactcacca
       61 gagtataatc ctatcgagaa aacatgggct catatcaaaa aacacctcag aagagtattg
      121 ccgaattgcg atacgtttct tgaggtactt ttatcctgtt cttgtttcag ttggctataa
      181 atagtgatta acagcttcaa gagcttcggt catataactt tgtccccacc atttactgcc
      241 aacacccaac ctaattcggc agcttggact ctttcatcaa ggctgacaac ggaaatatct
      301 ccaatggctt gctgattttc tttgagctca attacccacc tgtatgtttt ttcctgtgaa
      361 taggattgaa gacagaactg gacatagtct tcagtatctt ggagtgtctg atgagttggc
      421 caggtcagat aggtggtaac cttttcatct gaagtccagt tttggaaaac tgattcata
//
LOCUS       DATUCG010000284          446 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.290, whole genome shotgun sequence.
ACCESSION   DATUCG010000284 DATUCG010000000
VERSION     DATUCG010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.290"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            57..422
                     /gene="mscL"
                     /locus_tag="VCA20_002485"
     CDS             57..422
                     /gene="mscL"
                     /locus_tag="VCA20_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEP1836164.1"
                     /translation="MLKELKNFLFRGNIVDLAVAVVIGGAFGAIIKSFVNDIITPLIL
                     NPALKAANVQNIADLAWNGVKYGSFLSAVINFLIIGTSMFFVVKAAEKAMPKKVEEKA
                     PAGPTQEELLAEIRDLLKK"
BASE COUNT          144 a           65 c           95 g          142 t
ORIGIN      
        1 ttttttatgg gtataataga taaagtaaaa tatttatgaa aggacatatt taggatatgt
       61 tgaaagaatt gaaaaatttc ttgttccgcg ggaacattgt tgaccttgct gtagcggttg
      121 taattggtgg tgcgttcggc gcaattatca aatcatttgt aaatgatatc atcactccat
      181 tgattttgaa cccagctttg aaagcggcaa atgtacaaaa tattgctgac cttgcatgga
      241 atggtgtgaa gtacggtagt ttcttgagcg ctgttattaa cttcctaatc attggcactt
      301 caatgttctt tgttgttaaa gcagctgaga aagcaatgcc taagaaagtt gaagagaaag
      361 ctcctgctgg tccaactcaa gaagagttgc ttgcagaaat ccgcgatttg ttgaaaaaat
      421 aatcaataat atagtaaact gaaaca
//
LOCUS       DATUCG010000285          444 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.291, whole genome shotgun sequence.
ACCESSION   DATUCG010000285 DATUCG010000000
VERSION     DATUCG010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.291"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(1..444)
                     /locus_tag="VCA20_002486"
                     /pseudo
     CDS             complement(1..444)
                     /locus_tag="VCA20_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987985.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
BASE COUNT          141 a           90 c           90 g          123 t
ORIGIN      
        1 ccatagatgg taaccttctt ggtctaagtc cttcttgagt tcactgctca ggtagcctaa
       61 atcacctagg ataaccgatt gcttacaccc ttctaataat tcataaacca cccttgatgt
      121 catgaacaga ggctggcgtg acaacatagt tcaaaatata accagataag gtcactagca
      181 tatgaacctt gaagccgtag aaccaaagat gtttagaggc gttgtagcca atatcggcta
      241 atcctttaaa aatggtagct ctttgattac gaacgggttg gcaaagtggc aaaggaaaac
      301 tatctataat agctagttta tcagatggaa gcatttcact catagctttt cggattaatt
      361 ggacgagcca aatcaattgt tttgaccgac gattgaaacg acttctttct agtaaccgac
      421 cacaagggaa gaggtgcaaa tgtt
//
LOCUS       DATUCG010000286          441 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.292, whole genome shotgun sequence.
ACCESSION   DATUCG010000286 DATUCG010000000
VERSION     DATUCG010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.292"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            <1..153
                     /locus_tag="VCA20_002487"
     CDS             <1..153
                     /locus_tag="VCA20_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEP1836165.1"
                     /translation="LKYLSAEQGHRLLPLPPYSPEYNPIEKIWAHIKKHLRRVLPNCD
                     TFLEVL"
     gene            complement(150..>441)
                     /locus_tag="VCA20_002488"
     CDS             complement(150..>441)
                     /locus_tag="VCA20_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEP1836166.1"
                     /translation="INVAGLTFKYGPESSIGMVKGKKLLHIQSNGGVYAGQDPAAQYI
                     KSIFEFLGFEDIHQIFIEGQSADPSQAQAIFEEAMGKLIQFWKYSQLKQEQD"
BASE COUNT          129 a          103 c           80 g          129 t
ORIGIN      
        1 ctaaaatatt taagtgcgga gcagggacat agactgttac cccttcctcc ttactcaccg
       61 gaatataatc ccattgagaa aatatgggct cacatcaaaa aacacctcag aagagtattg
      121 ccaaattgcg atacttttct tgaggtactt tagtcctgct cttgtttcag ttgactatac
      181 ttccaaaatt gtatcaattt ccccatagct tcttcaaaaa tagcctgagc ctggcttgga
      241 tcggccgatt gtccttcgat aaaaatctga tggatatctt caaagcccag aaactcaaag
      301 atagacttga tgtattgggc tgcaggatct tgaccagcgt aaactccacc attggactga
      361 atatgcaaca attttttgcc cttgaccata ccgatagagc tctcaggtcc atacttgaat
      421 gtcagtcctg caacgttaat g
//
LOCUS       DATUCG010000287          428 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.293, whole genome shotgun sequence.
ACCESSION   DATUCG010000287 DATUCG010000000
VERSION     DATUCG010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.293"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(25..327)
                     /locus_tag="VCA20_002489"
     CDS             complement(25..327)
                     /locus_tag="VCA20_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1836167.1"
                     /translation="MYEFVLEYGSFPVKLIDGFVNNRSEVPDFLKEDDEMIARLNQIN
                     ELFHQLFLTIECKFDYIGKQFPDKIEHIRTLYHPLADDLLAKYSNQIELKIEPLIL"
BASE COUNT          110 a           65 c           97 g          156 t
ORIGIN      
        1 gatgattgtg tcatctcttt tgtcttacaa aataaggggt tcaatcttta actctatttg
       61 gttgctatac ttggctagta aatcgtctgc caggggatga tagagtgttc ttatgtgttc
      121 aatcttgtca ggaaattgtt ttccgatgta gtcaaattta cactcgatgg ttaaaaagag
      181 ttgatggaag agttcgttga tctggttcag gcgggcaatc atctcgtcat cctctttcag
      241 aaaatctgga acttctgagc gattgttgac aaagccgtcg atgagtttta cggggaaaga
      301 gccgtattct aggacaaatt cgtacatagg gttctcctta tcattgttag gactattgta
      361 gcatagtttg gagtgtgaca aaaggatttc aactttattg ctgtttaagt tttgtttaac
      421 attatttt
//
LOCUS       DATUCG010000288          415 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.294, whole genome shotgun sequence.
ACCESSION   DATUCG010000288 DATUCG010000000
VERSION     DATUCG010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.294"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
BASE COUNT          168 a           66 c           36 g          145 t
ORIGIN      
        1 cacaaaataa cactcgcaaa ttgaaaaata atgatgctac ggaaagaatt tattatatct
       61 aacttatata aaataatatt acttatacat atgataataa gagaaaatat acttcttact
      121 accatagggt atagataaat ttttgctatc attctttttg tgtatcccag atgtttcaat
      181 gcagcaatat caccaacaga atttttcaac attaactcaa ataatcctgt caaaaaacaa
      241 actgtaaata taagtaaaat aagtagcact accacactca aacttataag tttttccaca
      301 ctagtatcta aatcatcata atattcaaat gcgtgattta cattataatt attattggat
      361 aatatcttcg ctatatctct tatatcttca atagattcaa cattaatata tattt
//
LOCUS       DATUCG010000289          398 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.295, whole genome shotgun sequence.
ACCESSION   DATUCG010000289 DATUCG010000000
VERSION     DATUCG010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.295"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            46..398
                     /locus_tag="VCA20_002490"
                     /pseudo
     CDS             46..398
                     /locus_tag="VCA20_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017284794.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT          120 a           89 c           73 g          116 t
ORIGIN      
        1 actttagagt tctcaaattg acaaacttat atatgtcctg cttctaattc actcgactat
       61 atcttcacag cactatcacg aaaagcttct cctcagtgga ttttagaagg cgacatcaaa
      121 ggctgttttg acaacatcaa tcatgactgg ctcttagaga acattccgat ggataaatct
      181 atcttaaaac aattccttaa agcaggtttt gtgttcaagg gtgagttatt cccaacggaa
      241 gacggcacac cacaaggtag tatcatctca cctattctcg ccaatatggc actagatggg
      301 ctacaaaaag tcttgtcgga taggtttcat accaatcgtc tcgggagaac tgaccttcga
      361 ttcaagaata gccataaggt caatcttgtc cgttatgc
//
LOCUS       DATUCG010000290          387 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.296, whole genome shotgun sequence.
ACCESSION   DATUCG010000290 DATUCG010000000
VERSION     DATUCG010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.296"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            <1..>387
                     /locus_tag="VCA20_002491"
     CDS             <1..>387
                     /locus_tag="VCA20_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEP1836168.1"
                     /translation="MSEQLEIEVNQDRVKHGKKPLKPVEKREVKNQKLSTTDPESGWF
                     HKGDHKEVFAYSAQVACDKYGWALAYSVEAGNIHDSQAFPALFAKLEPLKPKSIIADS
                     GYKTPSIAKFLLDKEITPVLPYTRPR"
BASE COUNT          112 a           92 c           86 g           97 t
ORIGIN      
        1 tcatgagtga acagctggaa atcgaagtta atcaagatcg ggtaaagcac gggaaaaagc
       61 cactaaagcc cgtcgaaaaa agagaggtta aaaaccagaa actatccact accgaccctg
      121 agagtggctg gttccacaaa ggagaccata aggaggtatt tgcttattct gcccaagttg
      181 cctgtgataa gtatggctgg gcacttgctt atagtgttga ggctggcaat atccatgaca
      241 gccaggcttt tcctgcactt tttgccaagc ttgaaccctt gaaacccaag tctatcattg
      301 cggattcagg ctataaaact cccagtattg ccaagttttt gcttgataag gaaatcactc
      361 ctgtccttcc ctacacccgt cctcgtg
//
LOCUS       DATUCG010000291          378 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.297, whole genome shotgun sequence.
ACCESSION   DATUCG010000291 DATUCG010000000
VERSION     DATUCG010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.297"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            complement(3..>378)
                     /locus_tag="VCA20_002492"
     CDS             complement(3..>378)
                     /locus_tag="VCA20_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEP1836169.1"
                     /translation="YQTLRLAAEEQGKLAFLDTVKPSRLSHYLQAIQETTTFFQTHSN
                     VITMAIDNDDAGRVFCQKLSNIGLPVSQDLPPLQVLETKSDWNDIVKQQSELSLSDII
                     QTVQAQVNKNHPPPKRERVLEL"
BASE COUNT           98 a           73 c           75 g          132 t
ORIGIN      
        1 tatcacaatt ccaaaacacg ctctcgtttt ggtggaggat gattcttatt gacttgtgct
       61 tggactgttt ggataatatc acttaaggat agttcactct gctgtttcac aatatcattc
      121 caatctgact ttgtttcaag gacctgcaat ggtggtaaat cttgagaaac cggaagtcct
      181 atatttgaca gtttctgaca aaataccctt cctgcatcat cgttatcaat agccattgtt
      241 atgacgtttg aatgagtttg aaaaaaggtt gtcgtctctt gtattgcctg aagataatgg
      301 ctaagcctgc ttggttttac tgtatctaga aatgccaatt tcccttgttc ctcggctgct
      361 agacgtaagg tctgataa
//
LOCUS       DATUCG010000292          343 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.299, whole genome shotgun sequence.
ACCESSION   DATUCG010000292 DATUCG010000000
VERSION     DATUCG010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.299"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            45..257
                     /locus_tag="VCA20_002493"
     CDS             45..257
                     /locus_tag="VCA20_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP1836170.1"
                     /translation="MEMIRVNLDKVLKDRQMTSKDLAEQVGITEANLSILKTGKAKGI
                     RFNTLMSICRILDCQPGDILEYVREG"
BASE COUNT          121 a           70 c           75 g           77 t
ORIGIN      
        1 aaaggggcaa caacttgaaa ccgagaacag cgagtttatc tgatatggaa atgattcgcg
       61 taaacttaga caaggtcctc aaagaccgac aaatgacttc caaggactta gcagagcaag
      121 tcggcatcac cgaagccaac ctgtccattc tcaagacagg caaggcaaaa ggcattcgtt
      181 ttaataccct gatgagcatc tgccgaatcc tagactgcca accaggggat attttggaat
      241 atgtgaggga ggggtaaaca cataaaaaga gtataatact catttaaaat caaaaatagc
      301 cacctaccac tcaaagtgga tatggagcgt tttgattttg tta
//
LOCUS       DATUCG010000293          338 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.300, whole genome shotgun sequence.
ACCESSION   DATUCG010000293 DATUCG010000000
VERSION     DATUCG010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.300"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
BASE COUNT           96 a           63 c           62 g          117 t
ORIGIN      
        1 aattgattac cttgaaagcc gattttcagc ctacgaaagt tttgcagaat ggtttttaaa
       61 acgtaaatta ggcttttggg ggaaaatgat attcgcctac ctactctggc tagtctggtg
      121 gcttctcttt tcccaccctc actatatcat ctttttcttc tacggggtta tcctactttc
      181 tctaattatc atgctgatag aatggtggaa atatagaaaa tgaacagcaa aaaacaccaa
      241 ctttcgtcgg tgtttttaag gagatatgaa aaatatttag gattgactat agtatagcct
      301 acttttcaat acgctccatc ggtcttggga ctgatatt
//
LOCUS       DATUCG010000294          336 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.301, whole genome shotgun sequence.
ACCESSION   DATUCG010000294 DATUCG010000000
VERSION     DATUCG010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.301"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
BASE COUNT          119 a           40 c           54 g          123 t
ORIGIN      
        1 gttttgggtg accgaagtaa aatatctata aaagcctatt aaattaagct ttcaagaatc
       61 attttcatta aaaatagata cttataaccc aatcttataa atcatggatt accattagat
      121 taccaaaatt atgttttcgg agcttatgtt attgagaaac aatgtgtatt cactttactt
      181 ttttgtaagt tcattttata atgtatatac aaacggaggt aagtttgaaa aaaataaaag
      241 ctaattgatt tatagtttat cctaattttg ggcgcactaa aaagaggtgt tgaaacctga
      301 tttttgagat taagcgacat tgaatccgtg ttgttg
//
LOCUS       DATUCG010000295          336 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.302, whole genome shotgun sequence.
ACCESSION   DATUCG010000295 DATUCG010000000
VERSION     DATUCG010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.302"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
BASE COUNT          134 a           47 c           37 g          118 t
ORIGIN      
        1 tcactaaaca atatatacaa tattcaagtt caagcaaata attatttttt cgaaaacaat
       61 aagaattcta tacaaaaaaa cttcaaatac tatttcggag gatattcaaa atttgtcaaa
      121 aagtatgata tcaatttaac aaaagatttc atatataaaa atgattactt tacaactagg
      181 gaaatgtatc tactatctcc agcacactat ctatactata catttaaagt attcaaatta
      241 tgtagttaca tttgttcaga ggatattgta gacttttcaa ctgatagaat taaggtattt
      301 tattctggtc tcttagagtt cagcactgtt aataca
//
LOCUS       DATUCG010000296          309 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.303, whole genome shotgun sequence.
ACCESSION   DATUCG010000296 DATUCG010000000
VERSION     DATUCG010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.303"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            <1..>309
                     /locus_tag="VCA20_002494"
     CDS             <1..>309
                     /locus_tag="VCA20_002494"
                     /inference="COORDINATES: protein motif:HMM:NF037291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HEP1836171.1"
                     /translation="VRYEGLVPGAKYTLTGKLMKIENGEAVETGHTKTMEFDADQSGS
                     GEVSLSFENVEVEVGTKYVVYEKAESKESYDYAEGKKKHQASHEDKNDKSQTFNVEKE
                     "
BASE COUNT          124 a           40 c           88 g           57 t
ORIGIN      
        1 agtacgttac gaaggattgg tgcctggagc gaagtataca ttgacaggaa agttgatgaa
       61 gatagaaaac ggagaagctg tagaaacagg acatacaaaa acgatggaat ttgatgcaga
      121 ccaaagcgga agcggagaag tcagcctaag ctttgagaat gtagaagtgg aagtcggaac
      181 gaagtatgtc gtatatgaaa aggcagaatc taaggaaagt tatgactacg cagaaggtaa
      241 gaagaaacat caggcatccc atgaggataa gaatgataag tcgcagacct ttaatgtaga
      301 gaaagagaa
//
LOCUS       DATUCG010000297          303 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.304, whole genome shotgun sequence.
ACCESSION   DATUCG010000297 DATUCG010000000
VERSION     DATUCG010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.304"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            5..>303
                     /locus_tag="VCA20_002495"
     CDS             5..>303
                     /locus_tag="VCA20_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEP1836172.1"
                     /translation="MLSKYVGRLTSCIENEITKTRNHSRQRGNIMRAVFGIDVSKASS
                     EVAIVINSEKIHGYSMTNDAIGFSRLLEDLKTVSNPEIVFEATGVYSRRLQAFLD"
BASE COUNT           93 a           46 c           69 g           95 t
ORIGIN      
        1 tagaatttta tcaaagtatg tgggacgctt gacgtcgtgt attgaaaatg aaatcactaa
       61 aacaaggaat cattcaagac aaagaggtaa tatcatgcgt gcagtatttg ggattgatgt
      121 gagtaaggca agttcggaag tagctattgt tatcaatagt gagaaaattc atggttattc
      181 aatgaccaat gatgccatag ggttttctcg cttgttggaa gacttgaaaa ctgtctctaa
      241 tcctgagatt gtcttcgaag caacaggtgt ctattctcgt cgtcttcaag cctttctgga
      301 taa
//
LOCUS       DATUCG010000298          296 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.305, whole genome shotgun sequence.
ACCESSION   DATUCG010000298 DATUCG010000000
VERSION     DATUCG010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..296
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.305"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            <1..>296
                     /locus_tag="VCA20_002496"
     CDS             <1..>296
                     /locus_tag="VCA20_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEP1836173.1"
                     /translation="IPEYQCLTSVPGVGKVYAAGIIAEIGQIERFKDHPQVAKYAGLN
                     WKQYQSGNTNSQNTELVKRGNRYLRYYLVEAANSVRRHDSEYQTFYMKKYQEVP"
BASE COUNT           95 a           56 c           62 g           83 t
ORIGIN      
        1 tcattcctga ataccagtgt ttaacttctg tgcctggtgt tgggaaagtt tacgctgcag
       61 gaattatcgc tgaaattgga cagattgagc gatttaaaga tcatcctcaa gtcgctaaat
      121 atgcaggatt gaattggaaa cagtatcaat ctgggaacac taactctcaa aatacagaac
      181 ttgtaaaacg aggcaatcgc tatcttcgct attacttagt tgaagccgcc aactcagtga
      241 ggcgacatga tagtgagtat caaacctttt acatgaagaa gtatcaagaa gttcct
//
LOCUS       DATUCG010000299          281 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.308, whole genome shotgun sequence.
ACCESSION   DATUCG010000299 DATUCG010000000
VERSION     DATUCG010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.308"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            <1..>281
                     /locus_tag="VCA20_002497"
     CDS             <1..>281
                     /locus_tag="VCA20_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEP1836174.1"
                     /translation="SRFYQNLTEDIVRAKNRLHKVLQVTFPELENILSTPSGEQYWNL
                     VIAFPCKDCVLELSKDKLSESIRQSTSKRISDKRVAYLAEKLIALANQS"
BASE COUNT           86 a           63 c           56 g           76 t
ORIGIN      
        1 agccgtttct atcagaatct gaccgaggat attgttcgag ctaaaaaccg cctgcacaag
       61 gtcttacaag tcactttccc tgaattggaa aatatcttat cgacaccatc tggcgaacaa
      121 tactggaact tagttatagc ttttccttgt aaggactgcg tacttgagtt aagcaaggat
      181 aaactctcag aaagcattcg tcaatccact tcaaaacgga tttcggacaa gcgtgtggcg
      241 tacttagccg agaagctgat agcactagct aatcaatctt a
//
LOCUS       DATUCG010000300          277 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.309, whole genome shotgun sequence.
ACCESSION   DATUCG010000300 DATUCG010000000
VERSION     DATUCG010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.309"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
BASE COUNT           86 a           45 c           57 g           89 t
ORIGIN      
        1 tatgaaacgt ggactcaagt attcatttat cctagtgaca gttggcattt gttagcctta
       61 cttgtgattt tactgttggc gcctttgcta tattaggatt gattaacaaa caaaaacata
      121 ctaagattag tagtgaggaa atctccgacg agagaaagta ctcactactt tttccttatg
      181 ataaagtaga ggtgtcttgt taagtcgtag ggctttttga cgctagacgt cgcaacaaaa
      241 actggcaaga cacctgtttt agaaagaatg ttatcaa
//
LOCUS       DATUCG010000301          265 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.denovo.310, whole genome shotgun sequence.
ACCESSION   DATUCG010000301 DATUCG010000000
VERSION     DATUCG010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..265
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.denovo.310"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            <1..>265
                     /locus_tag="VCA20_002498"
     CDS             <1..>265
                     /locus_tag="VCA20_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HEP1836175.1"
                     /translation="PSHDALERVVSIVNPDFIKELKLSFEASSETTDFSKLIAVDGKT
                     IRGNRGKHQSPTHIVTAYDGGNRISLGQVAVEDKSNEITAIPRL"
BASE COUNT           78 a           55 c           60 g           72 t
ORIGIN      
        1 tccttctcat gacgccttgg aacgtgttgt gagtatagtt aatccagatt tcataaaaga
       61 actgaaactg tcctttgaag ctagttcgga aacaactgat ttttcaaaat tgattgccgt
      121 tgacggaaaa acgattcggg ggaaccgagg gaaacaccaa tccccaactc atattgtcac
      181 tgcttatgat ggtggtaatc ggattagcct tgggcaggta gctgttgagg ataaaagcaa
      241 tgaaatcaca gccattcctc gcctc
//
LOCUS       DATUCG010000302        23010 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.guided.019, whole genome shotgun sequence.
ACCESSION   DATUCG010000302 DATUCG010000000
VERSION     DATUCG010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23010
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.guided.019"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            128..1126
                     /locus_tag="VCA20_002499"
     CDS             128..1126
                     /locus_tag="VCA20_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004192896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEP1836176.1"
                     /translation="MKVLLYLEGKTVLEKSGIGRALQHQMEALDMAGIPYTTDLLGDY
                     DVIHINTYGPRSWLLLHAAKRSGKKVIMHGHSTKEDFQNSFIGSNTLAPFVGKYLASM
                     YQKADFVITPSEYSKKLIQSYGVTTPIIAVSNGIDLDKYKKDERKEAIFREHFKIEEG
                     QPVVVCAGLYFRRKGIEDFVKVAEKMPHVRFIWLGSINKWLIPSYIRKIVEGDHPANV
                     EFPGYFKGAVFQGAMSGADAFFFPSYEETEGIVVLEALASHQHVVLRDIPVYDGWIDE
                     NVAELGHTVDDFVDSIQKILDKQVDKREAGYQVAVSRSIDNVSHQLVDAYRQVLEM"
     gene            1127..2440
                     /locus_tag="VCA20_002500"
     CDS             1127..2440
                     /locus_tag="VCA20_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEP1836177.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKTELEKLGHTVFIFTTTDED
                     VNRYEDWDIIRIPSVPFFAFKDRRVAYAGFTDTLKIASRYKLDIIHTHTEFSLGILGK
                     MIAKELDIPVVHTYHTQYEDYVHYIAKGRIIRPGMVKYLVRAFLKDLDGVICPSEIVE
                     DLLMSYNVPISKRVIPTGIDLVKFDRPEIGKDETDELREQLGIASDETMLLSLSRISH
                     EKNIQAIVQAMPKILLENPKVKLVIVGGGPYTDDLQEMIAELNLDEHVQLTGMIAPSD
                     TALYYKAADFFISASTSETQGLTFLESLASGTPILAHSNPYLKNVITDKMFGTLFIRE
                     QELADAVVEAIVATPPMNPYVLDKKLYEISSTNFGRKVFEYYLDLKISYDFRKEHTNQ
                     DSVAEALIKEAIYLPQKVVVKSTDTTAKILKKSVEQVKSIRNFFE"
     gene            2455..2829
                     /locus_tag="VCA20_002501"
     CDS             2455..2829
                     /locus_tag="VCA20_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1836178.1"
                     /translation="MERLQMESWKSLVLATLIVLSMYAGIYLIAFHLISDTMLSKELV
                     VGNILLYTPIVLFLTGLLYGFFHGFSPTLLALILLLYPVTIFFFKEWIILYQIVYTLC
                     ALIGNGVGALLFSLRKKMRKNP"
     gene            3262..3447
                     /locus_tag="VCA20_002502"
     CDS             3262..3447
                     /locus_tag="VCA20_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HEP1836179.1"
                     /translation="MNRLIMTKQGRYYDETPYTLEHKMAENIWWLIELADRLDIDIQK
                     EMETFLAQKEELLGIKK"
     gene            3597..5546
                     /gene="thrS"
                     /locus_tag="VCA20_002503"
     CDS             3597..5546
                     /gene="thrS"
                     /locus_tag="VCA20_002503"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018164389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEP1836180.1"
                     /translation="MLKITFPDGAVREYQAGVTTFEIAESISKSLAKKALAGKFNGKL
                     IDTTRAIEEDGTLEIVTPDHEDALDILRHSAAHLFAQAARRLFPDIKLGVGPAIQDGF
                     YYDTDNAAGQISNEDLPRIQEEMMKIVKENFPSERREVTKEEALEIFKNDPYKLELIH
                     EHSDDEGGLTIYTQGEYVDLCRGPHVPSTGRIQIFELVNVAGAYWRGKSENPMMQRVY
                     GTAWFDKKDLKAYLQMREEAKERDHRKLGKELDLFMISQEVGQGLPFWLPNGATIRRT
                     LERYITDKELASGYQHVYTPPLASVELYKTSGHWEHYSEDMFPTMDMGDGEEFVLRPM
                     NCPHHIQVYKNHVRSYRELPVRIAELGMMHRYEKSGALTGLQRVREMTLNDGHIFVTP
                     EQIQEEFKKALQLIIDVYADFNLNDYRFRLSYRDPEDKEKYYDNDEMWENAQRMLKGA
                     MDEMGVDYFEAEGEAAFYGPKLDIQVKTALGNEETLSTIQLDFLLPERFGLTYIGADG
                     EEHRPVMIHRGVISTMERFTAILIETYKGAFPTWLAPTQVTMIPISVEAHLDYAWKVA
                     KELQDRGVRVHVDERNEKMQYKIRQSQTSKIPYQLIVGDKEMEDNAVNVRRYGSKATQ
                     TQSVSEFVDHILADIARKSRPAETE"
     gene            complement(5630..6673)
                     /locus_tag="VCA20_002504"
     CDS             complement(5630..6673)
                     /locus_tag="VCA20_002504"
                     /inference="COORDINATES: protein motif:HMM:NF013884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEP1836181.1"
                     /translation="MKRLIYIDQLKGFGILAVLFLHTVGYIRNALPYTDIPLWRLEAW
                     LHSFLMPFFVFVSGFSFYHFYLKPKPRNNTYIYIYIFRLLQIYAVFGSSIVISKILFS
                     SYTVDKIQPSHLVSNFLLPDTSMWYIWFLILMATSTLILHRLQLTKPVSLICIGLFTL
                     FAVLWGGPLNISNLPIGLQHLTSLPLFYFLGIYFAKKEIHKKMVRQTVFHSLAGLVTI
                     FYLIYLQSYQTYPSQHLPFHTFLRYANGLAMTYLIVSIIITLERRLPAVISWFGQCSL
                     YFYLIHTYILTILTLFIKKYFLDFPTPVLLLISFGLLLIINSLVLMIVKRIPILDKVF
                     QGKVISYPQKKSN"
     gene            complement(6796..7455)
                     /locus_tag="VCA20_002505"
     CDS             complement(6796..7455)
                     /locus_tag="VCA20_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009659320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEP1836182.1"
                     /translation="MNYVLELLKPSTFQLLWSGLKLTLYISSISVILSILFGMILAIM
                     RNGKNPIFKWTATIYIEFVRNVPNLLWIFTVFLVFQMKSTPAGITAFTIFTTAAMAEI
                     IRGGLNAIGPGQTEAGLSQGFTSVQIMVYIIMPQAIRKMLPAIISQIVTVIKDTSFLY
                     SVIALQELFGSSQILMGRYFEAEQVFALYLMVALIYFSINFAISSLSRYVAKSWASSI
                     E"
     gene            complement(7470..8168)
                     /locus_tag="VCA20_002506"
     CDS             complement(7470..8168)
                     /locus_tag="VCA20_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEP1836183.1"
                     /translation="MTVLTTSPYAWENWVSYFKDFPTFCQGFLFTLAISVGAFIMAMF
                     LGIVFGSLSSSKNKVLKIIARVYVEYYQNTPLLVQFMIVYYGLPLISNYVLMPSIYWT
                     AVICVGLYHGAYIAEVIRAGIEAVPTGQTEAALSQGFTQSETMRIIILPQALPTILPP
                     LTNQVVNLIKNTATVAIISGADIMFMTKSWSAMNANYIPAFAGAAFLYFIMCFPVASW
                     GRRIEEKNKSAFSH"
     gene            complement(8183..9022)
                     /locus_tag="VCA20_002507"
     CDS             complement(8183..9022)
                     /locus_tag="VCA20_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEP1836184.1"
                     /translation="MDKKKINIFFAIILLAFGLILLVFAKQPVAQTETPASITNSDQV
                     QAIIDRGVLRVGVKQDVPNFGYKNPDTGEFEGMEIDIARKIADELGVEIEFTPVTAQT
                     RGPLLDNGQVDMVIATFTITEERKLLYNFTTPYYTDAVGFLVNKDSGIKTFTDLDGKT
                     IGVAQGSITRTLISELADKYGISVNFAELGSYPELSVSLRAHRTDAFSVDQSILSGYI
                     SSKSELMDFSFSASDYGIVTKLSNKDLNDYLNSLVEKWTADGSLQSIYDANGLKPVTE
                     TDE"
     gene            complement(9033..9794)
                     /locus_tag="VCA20_002508"
     CDS             complement(9033..9794)
                     /locus_tag="VCA20_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEP1836185.1"
                     /translation="MPLVEFKDVNKYYGDYHALRNINLEFEPGQVVVLLGPSGSGKST
                     LIRTINGLEAIDEGTLIVNGHDISSTSIKELVSLRKEVGMVFQHFNLYPHKTVLENVT
                     LAPIKVLGINKATAEKTAQKYLEFVNLWDRKDSYPAMLSGGQKQRVAIARGLAMKPEL
                     LLFDEPTSALDPETIGDVLAVMQKLARDGMNMIVVTHEMGFAREVADRIIFMAEGEVL
                     VDTTDVNGFFDNPTEPRAQQFLSKIINHESDKVKL"
     gene            9988..10692
                     /locus_tag="VCA20_002509"
     CDS             9988..10692
                     /locus_tag="VCA20_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEP1836186.1"
                     /translation="MKKILIVDDEKPISDIIKFNMTREGYEVVTAFDGREALEVFEAE
                     FPDIVILDLMLPELDGLEVARTIRKTSNVPILMLSAKDSEFDKVIGLEIGADDYVTKP
                     FSNRELQARVKALLRRSELAETQTNLESTGTPELVIGDLVILPDAFVAKKHGKELELT
                     HREFELLHHLAKHLGQVMTREHLLETVWGYDYFGDVRTVDVTIRRLREKIEDTPSRPE
                     YILTRRGVGYFIKGND"
     gene            10685..12034
                     /gene="vicK"
                     /locus_tag="VCA20_002510"
     CDS             10685..12034
                     /gene="vicK"
                     /locus_tag="VCA20_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HEP1836187.1"
                     /translation="MINQLRYLMTTAEFWFVVILIGFLIALTVLLIENYRDNKQIKQL
                     NQKVNALIEGNYADVLDMRGSPEITDMANSLNDLSEVIRLTHDNLEQEKTRLTSILSY
                     MSDGVIATDRIGRIIMINDMAQKQLGLSNPKQEQYHLLEVLDLSDRYTLRDLLAQTPE
                     IVIDHTNENEEFLTLRANFATIRSESGLISGLVVVLHDMTEQAKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALTESVAPSFVKVSLDETNRMMRMITDLLSLSRIDNQVG
                     QIDVELINFTAFVTFILNRFDQMKNADSDKVYTIVRDYQISPIWVEIDTDKMTQVLDN
                     ILNNAIKYSPDGGTITFSMKTTDSQLIVSVSDEGLGIPKADLPRIFDRFYRVDKARSR
                     AQGGTGLGLAIAKEIVKQHKGFIWAKSEYGHGSTFTIVLPYSKDIALDEWDDSDEEE"
     gene            12041..12844
                     /locus_tag="VCA20_002511"
     CDS             12041..12844
                     /locus_tag="VCA20_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEP1836188.1"
                     /translation="MIGKGFNYSILASGSSGNCFYLETDKKKILVDAGLSGKKITSLL
                     AEIDRKPEDIDAILVTHEHSDHIHGIGVLARKYGMDIYANELTWQAMESKLGKIDVAQ
                     KHIFELGAMKTFGDLDIESFGVSHDAACPQFYRFMKDDKSFVMLTDTGYVSDRMVGIV
                     ENADAYLIESNHDIEILRSGSYSWNLKQRILSDKGHLCNEDGADAMIRSLGNRTKKIY
                     LGHLSKENNIKELAHMTMVNQLAQADLGVGVDFQVYDTSPDTATPLTKI"
     gene            13270..14586
                     /locus_tag="VCA20_002512"
     CDS             13270..14586
                     /locus_tag="VCA20_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEP1836189.1"
                     /translation="MSLQAGQKVGLVGNNGVGKSTFLKILLGLDRDFAGQIEVKADWA
                     YVPQLQERSSLSGGEQVWKSIQEAFAQRPQLLIMDEPTANLDQEHQEKLIKQIKRYRG
                     SLLVVSHDRHFLNQIASHIWHLEEGSIQVYTGNYEAFVESRRARREGQQQAYEAYQKK
                     VAQLKKAQHERQAKAQKMNKKKKGISSSEWKVNAMMGSYDSQAKSIAKSAKQLEKRIE
                     RLEKVSQPRKEAHIKMESKGALDTDLHRLFLLQAGQVLMDEVYLFDFPQIGMTFGEKL
                     ALVGANGSGKTSFVRKLISKELEGYYNPKLKIAYFAQDLINLDEEKTALENVSCTSLQ
                     DRMTILNLLGMLGLSYDKAQQKVANLSGGERVRLSLAKVLLADANLLILDEPTNFLDM
                     AAIEALETFLKEYEGSVLLVSHDQQFVETSVHSQWEIVQACLVKKS"
     gene            14678..16753
                     /locus_tag="VCA20_002513"
     CDS             14678..16753
                     /locus_tag="VCA20_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEP1836190.1"
                     /translation="MKKRKNKPLEHDGNFISLRLNILFSIVIFLFLVLILRLADMQII
                     NHDFYSNKLSTASQKIISNGSIRGQIYDAQGKPLVENEIQQVVSFTRPNKMSAQEMKE
                     VANKLLQWVNVSDVTITQRDKADYYLADEEVYRQVVEALPEDKKYDSDGNYLSESTIY
                     SNAVDSIPAEALQYSEQDSKAIELFKQMNGATYFSTVNLVTDTLTAEQVAHIVANESQ
                     LPGISTTNNWQRTILPTSLSSIIGTVTSEQAGLPAEDAEYYLSKGYSSNDRVGTAYLE
                     KQYEEVLQGQREKKQINLDRNGNVESIETIQEGQQGNNIKLTIDLAFQDGVNAILKKY
                     FESELATGSALYSEGVYAVALEPSTGAVLAMSGYSHEKGAGSITEDALGTITSVFTPG
                     SIVKGATISSGWENGVISGNQVQLDEPIYFAGSAPITSWYGAYGSFNIDATEALEYSS
                     NVYMVKIALGLLGQTYSANMYLNDGDTLTNAMTKLRSTFAEYGLGASTGIDLPLESTG
                     FLPEEYSTANFITNAFGQFDNYTPMQMAQYAATIANNGTRISPHLVEGIYGNNAQGGL
                     GELIETVSGKEMNQVNISAEDMSLLRQGFYQVVNGSGRFNTGSAIGRGAAVTISAKTG
                     TAETYTTTASGDVVTAVNTNVVAYAPSDNPQIAVAVVLPNLTNQNSTTTKTITQEIIN
                     LYQSLHPMN"
     gene            16762..17358
                     /gene="recR"
                     /locus_tag="VCA20_002514"
     CDS             16762..17358
                     /gene="recR"
                     /locus_tag="VCA20_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEP1836191.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMEDDVVNEFA
                     KNLLAAKRDLSYCSICGNLTDQDPCAICQDSTRDQSTILIVEDSRDVTALENIQEYHG
                     LYHVLHGLISPMNGIGPDDINLKTLLTRLMDNEVTEVIVATNATADGEATSMYISRVL
                     KPAGIKVTRLARGLAVGSDIEYADEVTLLRAIENRTEL"
     gene            17463..18509
                     /locus_tag="VCA20_002515"
     CDS             17463..18509
                     /locus_tag="VCA20_002515"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEP1836192.1"
                     /translation="MSKETLILLYGGRSAEREVSVLSAESVMRAINYDKFFVKTYFIS
                     QTGDFIKTQEFSQTPSADEKLMTNATIVEEQKIRPSDIYEEKAVVFPVLHGPMGEDGS
                     IQGFLEVLRMPYVGTNILSSSVAMDKITTKRVLESAGISQVPYVAVIEGENIDDKIAE
                     IEGKLTYPVFVKPANMGSSVGISKAEDLAGLRKALDLAFKYDSRVLVEQGVNAREIEV
                     GLLGNADVKTTLPGEVVKDVAFYDYDAKYIDNKITMEIPAKIDESIMDIMRDNAAKAF
                     RAFGGCGLSRCDFFLTEDGDIFLNELNTMPGFTQWSMYPLLWDNMGLAYPDLIEELVR
                     LAKEMFEKRESHLI"
     gene            18597..18977
                     /locus_tag="VCA20_002516"
     CDS             18597..18977
                     /locus_tag="VCA20_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEP1836193.1"
                     /translation="MYQTTVKGEGLFQALSQGYGEPVRTFGLTEKGETPVSLVNIGLA
                     ACITMCVQGYYASQEGNKTMPVQVESRLDNQRFEVLIGIGESVSAQKQKEILAYVEKK
                     CKVTALLKEDLQFETSFFVLESVE"
     gene            18978..20054
                     /locus_tag="VCA20_002517"
     CDS             18978..20054
                     /locus_tag="VCA20_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP1836194.1"
                     /translation="MFLAIEEMRQNKLRYGLILGLLVLISYLVFFLTGLAYGLMQENR
                     TAVDKWQADYVLLNSESNRLITASKIDTDLLDQVDASNKALIRQQAGVAYVDENATSD
                     EKEKVNIFAVESDSFIVPNIVEGRLYEKTGEVLVDKTLSEVEGFGLGDQIYLSGSDEK
                     VTIVGYTDNAYFGVTPVIYMDFTAFAELMQADQQQAATSNLASAIVVRGDVASLPNEL
                     EKVAIADFIENLPGYKAQNMTFGFMIGFLIVISAIVIGIFIFVLTTQKSPIFGLMKIQ
                     GLSNGYISGSVLAQTFLLAGVGTVLGLAGTYLSSLVLPSAVPFENNWTFYIVIGLALV
                     VFALLGASFSVRSIFKVDPLRNLS"
     gene            20064..20732
                     /locus_tag="VCA20_002518"
     CDS             20064..20732
                     /locus_tag="VCA20_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1836195.1"
                     /translation="MKTLIFENISKTFQDGSQTITALKPTNFSIEEGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPTTGRVLINQSDYSDLPEKKRAKLRYKDIGFVLQASNLIPFLTV
                     EKQLTLVDKVNKKAEPSKRNELLSELGVDHLKNKFPKDLSGGERQRVAIARALYNDPA
                     LILADEPTASLDSNRAFEVVELLAKEAKERNKSIIMVTHDQRMIEKSDKVYEMKDGVL
                     TQVR"
     gene            20787..22148
                     /locus_tag="VCA20_002519"
     CDS             20787..22148
                     /locus_tag="VCA20_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEP1836196.1"
                     /translation="MKLTLHEVAQVLGAKNDISLYPDTALNKLEFDSRLITEGDLFVP
                     LKGARDGHDFIPVAFENGCAVTLSEVSLDVPYILVEDCLAALQQLAAYYLEKTGVEVI
                     AVTGSNGKTTTKDMIHDVLATTYKTYKTQGNYNNEIGLPYTVLHMPDDTEKLVLEMGQ
                     DHLGDIHLLSEIAKPSLSVITLFGEAHLEFFGSREEIAKGKLQIADGMAAGNKLLLPA
                     DPIADKLLPSHVDLIRFGEQGDLQVTSLVESKDSLTFTVNFMDGDIFLPVTGKYNATN
                     AMVASYVGKTLGVPDEAIKSALAGLNLTRNRTEWKKAANGADILSDVYNANPTAMRLI
                     LETFSSIPANEGGKKIAVLADMKELGSQSVDLHNQMILSLLPDVLDTVIFYGQDIEGL
                     AQLASQMFPIGKVYFFRKNAEQDQFEDLVKQVRESLGKEDQILFKGSNSMNLGKVVEA
                     IEK"
     gene            22239..22922
                     /locus_tag="VCA20_002520"
     CDS             22239..22922
                     /locus_tag="VCA20_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HEP1836197.1"
                     /translation="MSDFFSNQISEAPNLDVLEHLFLMIIWFFMLYLCFRCYQKRWFH
                     IVFWWLQFIQIVSLYTWYIVADFPLSESLPFYHCRLAMLFILWAKPGPLKRYFAYLGL
                     FGSLSAFIHPVFDPFAFPHLTFFTFVIGHYALTVNCMLYLLSDLEGEMLKGKEVVKYT
                     LIMNMLILGVALLTGGNYGFLRQAPLVNTNNLPLNFFLVTCLLCFSILSIQAMLIAYL
                     KKESKQMDS"
BASE COUNT         6819 a         4178 c         5313 g         6700 t
ORIGIN      
        1 atatcataaa tgtattgttt ttgtttcatt taactatatt ctttagtatt ggttttaatt
       61 gatacaaaat tcgtgtagaa tacagtttaa aatatgaaaa aatgtaaaaa aggtctataa
      121 tggtattatg aaagtactat tgtatttaga aggtaaaact gttttagaga aatcaggaat
      181 tggccgggca cttcaacacc agatggaagc gctagatatg gcaggcattc cttatacaac
      241 agacctgctg ggagattatg atgtcattca tatcaatacc tatggtccac gctcttggtt
      301 attgctacat gcagccaaac ggagtggaaa aaaggtaatt atgcatggac attccacgaa
      361 ggaagatttt caaaattcct ttatcggttc gaatacccta gctccttttg tagggaaata
      421 tctagcaagc atgtaccaaa aggcagactt tgtcatcact ccttcagagt attcaaaaaa
      481 actcatccag tcttacggtg tgacaacgcc aattatagcc gtttcgaatg gtattgattt
      541 agataaatat aagaaggatg aacgtaagga agcaatcttt cgagagcatt ttaaaattga
      601 agaaggtcag ccagttgttg tctgtgcagg actttacttc cgacgtaaag gaattgaaga
      661 ctttgtcaag gtagccgaaa agatgccgca tgtccgcttc atttggctcg gttcaatcaa
      721 taagtggctg attccgtcat atattagaaa aattgttgag ggagatcatc cagcgaatgt
      781 cgagtttcca ggttatttta aaggtgctgt ttttcaaggt gcaatgagtg gagcggatgc
      841 ctttttcttc ccatcgtatg aggaaacaga agggattgtt gttctggaag ctcttgctag
      901 tcatcagcat gttgttctaa gagatattcc agtttacgat ggttggattg acgaaaacgt
      961 tgccgaattg ggacatacag tggatgattt tgtcgattct attcagaaaa tcttagacaa
     1021 gcaggttgat aaacgtgagg cgggctacca ggttgcggtt agtcgctcga ttgacaatgt
     1081 ttcccaccaa ttggtggatg cctatcgaca agtattggag atgtaaatgc ggattggatt
     1141 gtttacggat acctattttc cacaggtttc aggagtcgcg acttcgattc ggactcttaa
     1201 gacagagttg gagaaattgg gacatacggt atttattttt acgacgacag atgaggatgt
     1261 gaatcgctac gaagattggg acattatccg aattcctagc gtaccttttt ttgcctttaa
     1321 agatcgtcga gtggcctacg caggttttac agatacctta aaaattgcaa gtcgctataa
     1381 actagatatt attcatactc atacagaatt ttctttgggg attttgggga aaatgattgc
     1441 caaggaacta gatattccgg tggtacatac ttatcacacc cagtatgaag actacgtaca
     1501 ctatattgcc aaggggagga ttattcgtcc cgggatggtt aaatatttag tacgggcatt
     1561 tttaaaagat ttggatgggg taatttgtcc tagtgagatc gttgaagatc ttttaatgag
     1621 ttacaatgtt ccgatttcta aacgagtaat tccgacagga attgacttgg taaagtttga
     1681 tagaccagaa attggcaagg atgaaacaga tgagttgcgg gaacagctag gaattgctag
     1741 tgatgaaacg atgttgctaa gtctatctag gatctctcac gagaagaata tccaagcaat
     1801 cgttcaggca atgccaaaaa ttttgctgga aaatccaaag gtgaagttgg tcattgtcgg
     1861 aggtggacct tatacagatg atcttcagga aatgatagcg gagcttaatc tagatgaaca
     1921 cgtccagttg acgggcatga ttgcaccaag tgatacagca ctttattata aagctgcgga
     1981 tttctttatt tctgcttcga ccagtgaaac gcagggacta actttcttag aaagcttagc
     2041 ttctggaact cctattttgg cacatagcaa tccctactta aaaaacgtta taacagataa
     2101 gatgtttggg acactcttca ttcgagaaca ggagttggca gatgccgtcg ttgaggcgat
     2161 tgttgctaca ccaccaatga atccgtatgt gcttgataag aagctctatg agatttcttc
     2221 tacgaatttt gggcgcaagg tttttgaata ttatctagat ttgaagattt cgtatgattt
     2281 tagaaaagaa catactaatc aagatagcgt agcagaagca ttgataaaag aggctattta
     2341 cctaccgcaa aaagtagtag ttaaatcaac agacacaaca gcaaaaatat tgaaaaaatc
     2401 agtcgaacaa gtaaaatcaa tccggaattt ctttgaataa tttggaggtt tcaaatggaa
     2461 agattacaaa tggaaagttg gaagagtctg gttttggcaa ctctcattgt tctatccatg
     2521 tatgcaggta tttatctgat tgcctttcat ctgattagtg acaccatgct ttctaaggaa
     2581 ctggtggtcg ggaatatatt gctctatact cccattgtac tattcctaac aggtcttctt
     2641 tatggatttt ttcacggttt ttctcctacc cttcttgcct tgattttact gctttatcct
     2701 gtaaccatct ttttctttaa ggaatggatt attctctatc agattgtcta caccttatgt
     2761 gctctgattg gaaatggggt aggagctctg ctattttctc taagaaagaa aatgaggaaa
     2821 aatccttgaa aaatcaggaa atcctgttat aatagctttt agagacttat caattctagg
     2881 aaatctttga gagagtgtgc ggtgggtgca agcacataga ggataggatt gacactactc
     2941 ggtatgattt tatgcaaaca taaaagggtg gttcccttat cgccaatctg aggtagtcag
     3001 gctttttagt gtgactgcga atgaaggtgg aaccacgtta cgacgtcctt ttgtgagggc
     3061 gtcgtatttt cttttggagg aggaatgatg ctacctgatg acttattaag acggtgtcga
     3121 gatgtgcgtc gtgcttacag agatttggaa cttaagcacc acgacaaaga gtggtccatt
     3181 gaggagtgaa acagtctggg gatagactgt tgcagctcaa caacttaaaa taaagacttg
     3241 ttgaccaatg atattggcaa catgaaccgt ctgattatga ccaagcaggg tcgttactat
     3301 gatgaaacgc cttatacttt ggagcataaa atggctgaaa atatctggtg gttgattgaa
     3361 ctggctgacc gcctggatat tgatattcaa aaagaaatgg aaaccttttt agctcaaaaa
     3421 gaagagttat tggggattaa aaaataaaaa tagaaaggac tagggtctcc gtatttggtt
     3481 gaactgaata cgggctacgg actgggccaa aaagataaac gagaactaga cgcttgcgtc
     3541 gtcgttctgt ttcctatttt ggctgtgtcc gcttaacgcc ctttatatct tatattatgc
     3601 ttaaaattac tttcccagat ggtgctgttc gtgagtacca ggctggtgtg acgacttttg
     3661 aaattgcaga gagtatcagc aagtctttgg cgaaaaaggc tcttgctggt aagttcaatg
     3721 gtaaattgat tgacaccact cgtgctattg aagaggacgg cacgcttgaa atcgtaacgc
     3781 ctgaccacga ggatgccttg gacatcttgc gtcactcagc ggcgcacttg tttgcccaag
     3841 cggctcgtcg tcttttccca gacatcaagt tgggggttgg tccagctatt caggacggtt
     3901 tttattacga tacggacaat gcggctggtc agatttcaaa tgaagacctg ccacgcattc
     3961 aggaagaaat gatgaagatt gtcaaggaaa acttcccttc agaacgccgt gaggtgacca
     4021 aggaagaagc tcttgagatt ttcaagaatg atccatataa gctggaattg attcacgagc
     4081 actctgacga cgagggtggt ttgaccatct atacccaggg tgaatacgtt gacctctgtc
     4141 gtggtccaca cgttccgtca acaggtcgta tccagatttt tgaattggtc aatgtagccg
     4201 gtgcttactg gcgtggtaag agcgaaaatc caatgatgca acgtgtttat ggtacagctt
     4261 ggtttgacaa gaaagacctc aaggcttacc ttcaaatgcg tgaggaagcc aaagagcgtg
     4321 accaccgtaa acttggtaag gaattggatc ttttcatgat cagccaagaa gttggtcaag
     4381 gtttgccatt ctggttgcca aatggggcga ctattcgtcg taccttggag cgttacatca
     4441 cagacaagga attggcttct ggttaccagc acgtttatac gccaccattg gcttctgttg
     4501 aactctataa gacttcgggt cactgggagc attactcaga agatatgttc ccaactatgg
     4561 acatgggcga cggtgaggag tttgtgcttc gtccgatgaa ctgtccgcac cacattcaag
     4621 tttacaaaaa ccatgtgcgt tcttaccgtg aattgccagt acgtatcgct gagcttggta
     4681 tgatgcaccg ctatgagaaa tctggtgctc ttactggttt gcagcgtgta cgtgagatga
     4741 cgcttaatga cggtcatatt ttcgtgacac ctgagcaaat tcaagaagag ttcaaaaaag
     4801 ctctccagtt gattatcgat gtgtacgcag atttcaacct aaacgactac cgtttccgcc
     4861 tgtcttatcg tgacccagag gataaagaaa agtactatga caacgatgag atgtgggaaa
     4921 atgcccaacg catgttgaaa ggtgccatgg atgaaatggg cgtggactac tttgaggctg
     4981 agggtgaagc tgccttctac ggtcctaaat tggatattca agtgaaaact gcccttggta
     5041 acgaagaaac cttgtcaact attcagttgg acttcctctt gccagaacgc tttggtctga
     5101 cctatattgg tgctgacggt gaagagcacc gcccagtcat gattcaccgt ggtgttatct
     5161 cgactatgga acgcttcaca gccatcttga ttgaaaccta caagggtgcc ttcccaactt
     5221 ggttggctcc aacgcaagtg accatgattc caatctctgt ggaagctcat ctggattacg
     5281 cttggaaggt tgccaaggaa ttgcaagacc gtggcgttcg cgtacatgtg gatgagcgga
     5341 acgagaagat gcaatacaag atccgtcaga gtcagaccag caagattcca taccaactca
     5401 tcgtcggtga caaggaaatg gaagacaatg ctgtcaacgt ccgtcgctac ggaagcaagg
     5461 caacacaaac tcagtctgta tcagaatttg tggaccatat cctagcagat attgctcgca
     5521 agtcacgtcc agctgaaact gaataataat tataaggagg agtccgaaag ggcttctttt
     5581 tgacagcaaa aactcccaag cgattggctg ggagtgctag tatcacttat tagttacttt
     5641 tcttttgagg gtaggaaatc accttccctt gaaatacttt atccagtata ggaattcttt
     5701 tgacaatcat caagactagg gaattgatga taagaagtaa tccaaaactt atgaggagga
     5761 gtacaggtgt aggaaaatct aaaaaatatt tcttaatgaa aagagtgaga atagttagga
     5821 tataggtatg gattagataa aagtatagtg agcattggcc aaaccaggat atgactgcag
     5881 gcaatcttct ctctaaagtg attatgatag aaacgatcag gtaggtcata gctagtccgt
     5941 ttgcatatcg taggaaggtg tgaaatggca ggtgctgact tgggtaggtt tggtaggact
     6001 gtaaatagat gagatagaag atggtgacca gaccggctaa actgtgaaaa acggtctgtc
     6061 taaccatttt cttgtgaatt tctttcttgg caaaataaat acctaaaaaa taaaatagcg
     6121 gtaaagaagt caggtgttgt aacccgattg gtaagttgct aatatttaag ggaccacccc
     6181 ataggacagc aaatagtgta aaaagaccaa tgcaaatcag tgaaactggt tttgttagtt
     6241 gcaatcgatg gagaataaga gttgaggtgg ccataaggat taaaaaccaa atataccaca
     6301 tagatgtatc tggcaggaga aaattagaaa cgaggtgact aggttgtatt ttatcaacag
     6361 tgtaacttga aaagaggatt ttgctaatta caatactact gccaaagact gcgtatattt
     6421 gcaacagtcg aaatatatat atatatatat aagtattatt tctaggtttt ggttttaggt
     6481 aaaaatgata gaaactaaag ccactgacaa atacaaaaaa tggcataagg aaagagtgga
     6541 gccaggcttc tagtcgccaa agagggatat cggtatatgg aagagcgttt cggatataac
     6601 cgactgtatg cagaaataaa acagctaaaa ttccaaaacc tttcagctgg tcaatataga
     6661 tgagtcgttt cataagtttc tcctttattt aactctaaac tatttgggaa cagaggcatt
     6721 tccaaaatgg tttgagtaca ttttctttga ttatatcata aaaccagctt tctcgagaaa
     6781 actggttcag attgtttatt caatactgct cgcccaagac ttagccacat aacgtgacag
     6841 gcttgaaata gcaaagttga tgctaaagta aatcaaggct accatgagat agagggcaaa
     6901 aacttgttct gcctcaaagt agcgtcccat gaggatttga ctcgaaccga agagttcttg
     6961 cagggcgata acggagtaga ggaaactagt gtccttgatg accgttacaa tctgcgagat
     7021 gatggctggc agcatcttgc gaatggcctg cggcatgatg atatagacca tgatttgcac
     7081 tgaggtaaaa ccctgtgaca agccagcttc ggtctggccc ggtccgatgg cgttgagacc
     7141 accacggata atctcggcca tggctgccgt ggtaaaaata gtaaaagcag taataccagc
     7201 aggcgtggac ttcatttgga agactaagaa tactgtgaaa atccaaagca agtttggcac
     7261 gttacgaaca aactcgatat agatagttgc agtccacttg aaaattggat tttttccatt
     7321 tcgcatgatg gcgagaatca ttccgaaaag aatggacagg attaccgaaa tgcttgagat
     7381 ataaagtgtt aacttcaacc cactccacaa gagttggaag gttgaaggtt tgagtaattc
     7441 taaaacataa ttcatcgttc aacctccttt tagtgtgaaa aggctgactt attcttttct
     7501 tcaatgcgac gtccccagct tgcaacaggg aagcacatga tgaagtaaag gaaagcagca
     7561 ccagcaaagg ctgggatgta gttggcattc atagcagacc aagattttgt cataaacata
     7621 atatcagctc cagagatgat ggcaacagta gccgtattct taattaagtt tactacttgg
     7681 ttggtcaaag gtggaagaat ggtcggaagg gcctgtggta aaatgatgat tcgcatggtt
     7741 tcagactgag taaatccttg tgaaagtgcg gcttcagtct gcccagtagg cacagcctca
     7801 atccctgcac gaatgacctc tgcgatatag gccccgtgat agagtccgac gcagataaca
     7861 gctgtccagt agatggacgg catgagaaca tagtttgaaa tcagtggaag tccataataa
     7921 acaatcatga actgaaccaa aagtggcgtg ttctgatagt attcaacata gacacgtgcg
     7981 atgattttta aaactttatt tttacttgaa gagagactac caaagacaat tcctaaaaac
     8041 atggccatga taaaggcacc gacagaaatg gctaaggtaa agagaaaacc ttggcaaaag
     8101 gttggaaaat ccttgaaata actgacccaa ttctcccaag cgtaaggact agttgttaaa
     8161 actgtcatat tgtttcacct ttctattcgt cagtttctgt gactggtttg agaccattgg
     8221 catcatagat gctttgtagg ctaccatcgg cagtccattt ttctacgaga ctatttaagt
     8281 agtcgttaag gtctttgtta gataatttag taacaattcc atagtcagaa gctgagaagc
     8341 taaagtccat gagttcagat ttcgaactga tatacccaga aaggatagat tggtctactg
     8401 aaaatgcgtc tgtacggtga gcacgtagcg agacagagag ttctggataa gaaccaagct
     8461 ctgcaaaatt gacagagata ccatatttgt ctgctagttc agatatgagg gtacgtgtaa
     8521 tggaaccctg agctacaccg attgtctttc catccaggtc ggtaaaggtc ttaattccac
     8581 tatccttatt gaccaagaag ccaacggcat cagtgtagta aggagttgtg aaattgtaga
     8641 gcaacttacg ttcctcagtg atggtaaaag ttgcgatgac catatccact tgaccgttat
     8701 ctaagagtgg tccgcgagtt tgtgccgtaa caggggtaaa ttcaatctcc acccctaact
     8761 cgtctgcaat tttgcgagca atatcaatct ccattccctc gaattcacca gtatcagggt
     8821 tcttgtagcc aaagtttggg acgtcctgtt tcactccgac gcggagaacg cctctatcga
     8881 tgatagcttg aacttgatca ctattggtaa tggatgcggg tgtttctgtt tgagctactg
     8941 gttgttttgc gaaaacgaga agaataagtc caaaggccaa taatataatt gcaaagaaaa
     9001 tgttgatttt tttcttatcc atagacttcc tcctagagtt ttactttgtc agattcgtgg
     9061 ttgataatct tgctgaggaa ttgttgggca cgaggctctg tcggattgtc aaagaagcca
     9121 ttcacatctg ttgtatcaac taaaacttct ccctcagcca tgaagatgat gcgatcagct
     9181 acttcacggg caaagcccat ttcgtgtgtt acgacaatca tgttcatccc gtcacgtgcc
     9241 aatttttgca taactgcaag tacatcgccg atagtttctg ggtccaaggc agatgttggt
     9301 tcatcgaaga gtagaagctc aggtttcata gctaagccgc gagcgatggc aacacgttgt
     9361 ttttggcctc cggatagcat agcagggtag gaatccttcc gatcccagag gttaacaaat
     9421 tcaagatact tttgagcggt tttttcagct gtagccttat tgattcccaa aaccttaatc
     9481 ggtgccaagg taacattttc taacacggtt ttgtgtggat aaaggttgaa atgctggaaa
     9541 accatgccaa cttctttgcg aagtgatacg agctctttga tagaagtaga ggaaatatca
     9601 tgtccattga cgatgagcgt tccttcatca attgcctcta aaccgttaat cgtgcgaatg
     9661 agagtagatt ttccggatcc ggaaggtcca aggagaacga caacctgtcc aggttcgaat
     9721 tccaagttga tattgcgtag ggcgtggtag tcgccatagt atttgttgac atctttaaat
     9781 tctactaaag gcataaaatt ctccttgcat agtgtttttt aggtattacc atcttagcat
     9841 tgcctatata caatgtcaag taaattctga caatttaatg tattgtatgt aacaaaatgt
     9901 aacatcgttg tgtcagatgg ttttgaaata tgaatttttt aggtaagttt gatataataa
     9961 aagaaataaa gcaaaggtaa gatactaatg aaaaaaatat taattgtaga tgatgaaaaa
    10021 ccaatctcag atattattaa gtttaatatg acgcgtgagg gatatgaagt tgtgacagct
    10081 ttcgatggac gtgaagcctt ggaagtattt gaggctgagt ttcctgacat tgtcattttg
    10141 gacttgatgc taccagaatt ggacggacta gaggtcgctc gaacgattcg taagaccagc
    10201 aatgttccca tcttaatgct atctgctaaa gatagcgaat ttgataaggt tatcgggctt
    10261 gaaatcgggg cggatgatta tgtgaccaag cccttctcta atcgcgaatt gcaggcgcgt
    10321 gttaaggctc ttcttcgtcg tagtgaatta gcagagacgc agacaaatct tgagtcaaca
    10381 ggaactccag agttggtgat tggcgatttg gtcattctgc ctgatgcgtt tgtcgctaag
    10441 aagcatggca aagagctgga gctgacccat cgtgagtttg aattgctcca ccatctggcc
    10501 aaacacttag gtcaggttat gactcgagaa catctattgg aaacagtttg gggttatgat
    10561 tactttggtg atgtccgcac ggtggatgta acgattcgtc gtctgcgtga gaaaattgaa
    10621 gatacaccaa gcagaccaga atacattctt actcgtcgcg gagtgggata ttttataaaa
    10681 ggaaatgatt aatcaattac gttatttaat gaccacggca gaattttggt ttgttgtcat
    10741 tttgattggc tttctaattg ctctgacggt ccttttgatt gaaaactatc gggataataa
    10801 gcaaattaaa caactcaatc aaaaagtcaa tgccttaatt gaggggaatt atgcagatgt
    10861 gttagacatg aggggaagtc ctgaaatcac agatatggcg aattctctta atgatctgtc
    10921 cgaggtcatc cgcttaactc atgataattt agaacaagaa aagacgcgtc tgacttccat
    10981 cctgtcctat atgagtgatg gtgttattgc tacagaccgt atcggacgga tcatcatgat
    11041 taacgatatg gcccagaaac agttggggtt atctaaccca aagcaggagc agtatcacct
    11101 gctcgaggtt ttagatttat cagaccgtta tacattgaga gatttgttgg ctcagacacc
    11161 tgagattgtg attgatcaca ccaatgaaaa cgaagaattt ttgactttac gcgctaattt
    11221 tgccacgatt cgtagtgaga gtggtctgat ttctggtttg gtcgtcgtct tgcatgatat
    11281 gaccgagcag gccaaggaag aacgggaacg taggctgttt gtgtcaaatg taagtcatga
    11341 attgcgtacg ccattgactt ccgttaaatc ctatctagag gctctggatg agggagcgct
    11401 gacggagtct gttgcaccga gttttgttaa ggtatcctta gatgagacca accgcatgat
    11461 gcggatgatt acagacctcc taagcttatc gcgtattgat aatcaagttg gtcagataga
    11521 tgtcgaactg ataaacttta ctgcttttgt gacctttatt cttaaccgtt ttgaccaaat
    11581 gaaaaatgct gattctgata aggtctatac gattgttcgt gactatcaaa tcagtcctat
    11641 ttgggttgag attgatacag ataagatgac tcaggttttg gataatatct taaataatgc
    11701 cattaaatat tcgccagacg gcgggacaat taccttcagc atgaagacca cagatagcca
    11761 gttgattgta tccgtctcag acgaaggtct ggggattccg aaagcagatt tacctagaat
    11821 ttttgatcga ttctatcgcg tggacaaggc gcgatctcgt gcccaaggtg gtacaggtct
    11881 tggtttagct attgcaaaag aaatcgtaaa acaacataaa ggctttatct gggctaaaag
    11941 cgaatatggt catggctcaa cgtttacaat tgtcttgccg tacagcaagg acatcgcact
    12001 agatgagtgg gatgattcag atgaggaaga ataggagaat atgatcggaa aaggttttaa
    12061 ttatagtatt ttagcttcgg ggtccagcgg gaattgtttt tacctagaaa cagataaaaa
    12121 gaaaatccta gtggatgctg gcttatcagg gaaaaagatt accagtcttt tggcggaaat
    12181 tgatcgaaag cctgaggata ttgatgctat cctggtaacg catgaacata gcgatcacat
    12241 ccatggtatc ggtgttttag cacgtaagta tggcatggac atttatgcca atgaattgac
    12301 ctggcaggct atggagagca aattgggcaa gattgatgtg gctcaaaaac atatcttcga
    12361 attgggtgct atgaagactt ttggcgacct agatattgag tcatttggag ttagccatga
    12421 tgctgcatgc ccgcaatttt atcgttttat gaaggatgac aaatcctttg ttatgttgac
    12481 ggatacaggc tatgtcagtg accgcatggt tggaattgta gaaaatgccg atgcttattt
    12541 gattgaatcg aaccatgata ttgaaatttt gcgctcgggt tcttattcat ggaatttgaa
    12601 gcaacggatt ctatccgata aaggccatct ttgtaacgaa gatggagctg atgccatgat
    12661 tcgctcgttg ggaaatcgga ccaaaaagat ttacctaggg catttgtcaa aggaaaacaa
    12721 tatcaaggaa ttggctcaca tgaccatggt taatcagttg gcgcaggctg atttaggagt
    12781 tggagtggat tttcaagtct atgacacatc gccagataca gcgactccct tgaccaagat
    12841 ttgacaaatt cattgaactt gtttagaata aatatataaa atacgtgagg tgaagttaca
    12901 tgaataatta agctacttat tagtcgttct taaaacttgt tgaaaatcaa ttatttgata
    12961 atttagtctt ttagtttgct tttagtacta ggcaacgagc tgcaggctgt actgaagtac
    13021 ggcaaagcga gttaacgtag taataaaaga taaactaaat gactacacaa gggataagac
    13081 tgctcataag ggcttactat tcatgtgact ttttccatag ggaaaggtca tgttttgcat
    13141 ggcttttttc ttgttcattg agtagaatag gggccctttt tgtgaagaaa agaggtctat
    13201 atggaagtaa taaaatgtat tcgattagag aaggaatttg cgggacgtag tttgtttaca
    13261 atccagcagt tgagccttca agctggtcaa aaggttggtt tggttggaaa taatggtgtt
    13321 ggtaagtcta cttttttgaa aatcttactg ggactggata gggattttgc tggtcagatt
    13381 gaggtaaaag cagactgggc ctatgtaccc cagttacagg aaaggagctc gctttcaggt
    13441 ggggaacagg tttggaagtc tattcaagag gcttttgccc aaagaccaca gttgttaatc
    13501 atggatgaac cgactgccaa tctggatcaa gaacaccagg aaaagctaat caaacaaatc
    13561 aaacgctatc gcgggagtct actagttgtt agtcatgatc ggcattttct caatcaaata
    13621 gccagtcata tttggcattt agaagaagga agtattcagg tctacacagg gaattacgag
    13681 gcgtttgtag aaagtcgccg ggctaggcga gaaggacagc agcaggccta tgaagcctat
    13741 cagaaaaaag tcgctcaact aaaaaaagcc caacatgaac gtcaagcaaa ggctcagaag
    13801 atgaacaaga aaaagaaagg catttcttcg tcagaatgga aggtcaatgc tatgatgggt
    13861 tcctatgata gtcaagccaa gtcaatagct aagtctgcta aacagttaga gaaaaggatt
    13921 gagaggttgg aaaaggttag tcagcccaga aaggaggctc atattaaaat ggaatcaaaa
    13981 ggggccttag atactgattt acacagacta tttcttttgc aggctggtca agttttgatg
    14041 gatgaagtgt atctatttga tttccctcag atagggatga cgtttggaga gaagttagcc
    14101 ttagtggggg cgaatggttc tggaaagact agttttgttc ggaaattgat ttcaaaagaa
    14161 ttagaaggct attacaatcc aaaattaaaa attgcttatt ttgcacagga tttgataaac
    14221 cttgatgaag aaaagactgc tttggaaaat gttagttgta cctctcttca agataggatg
    14281 acgattctca atttattagg tatgttagga ctttcttacg ataaggctca gcagaaggta
    14341 gcaaatttgt ctggtggtga gcgtgttcgt ctctccttag ctaaggtatt gttggcggat
    14401 gcaaatcttt tgatattaga tgaaccgaca aattttctag atatggcagc tattgaagct
    14461 ttggaaacat tcttgaaaga atatgaagga agtgtcctct tggtttccca tgatcaacag
    14521 tttgtggaaa catctgttca cagtcaatgg gagatagtgc aagcttgtct cgtgaagaaa
    14581 tcataagaaa atattttctg aaaaggaggc tctgtctaaa aaatattttt tgcctgtggt
    14641 ataataagaa ggatatttct agaatgaggt ctttgaagtg aaaaaaagaa agaacaagcc
    14701 tttggaacac gatggaaatt ttatttccct tcgtttaaat attttatttt ctatcgtgat
    14761 ttttcttttt ctagttttga tattgcgttt ggcagatatg cagattatca atcatgattt
    14821 ttatagtaat aagctgtcta ctgccagtca gaaaatcatc agcaatggct ctattcgtgg
    14881 gcagatttac gatgcgcagg gaaaaccttt ggttgaaaac gagattcagc aggtcgtctc
    14941 cttcacacgt cccaacaaga tgtctgctca ggaaatgaaa gaagtagcta acaaactctt
    15001 gcagtgggtc aatgtgtcgg acgtaacaat tactcaacgt gacaaggctg actattattt
    15061 agcggatgaa gaagtttatc gtcaggtagt cgaggccttg ccagaagata aaaaatacga
    15121 ttctgatggc aactacctga gtgaatcgac aatttattct aatgcggttg atagtatccc
    15181 tgctgaagca ttgcaatatt ctgagcaaga cagcaaggca attgagctat ttaagcagat
    15241 gaatggagct acttacttct ctactgttaa tttggtaaca gatactctaa ctgctgaaca
    15301 ggttgctcat atcgtagcca atgaaagtca acttccaggc atttcaacga ctaacaactg
    15361 gcaacggacg attttaccaa cttccctcag ctctattatt ggtacagtaa cgagtgaaca
    15421 ggctggtttg cctgcggaag atgcggaata ctatctctcc aagggctact catcaaatga
    15481 ccgtgtggga actgcctacc tcgagaaaca atatgaagaa gtcttgcaag gtcaacgtga
    15541 gaaaaaacaa atcaacctag atcgaaatgg caatgtcgaa agtattgaga caatccaaga
    15601 aggacagcag ggaaataata ttaaactgac tattgattta gcctttcaag atggggtcaa
    15661 tgctattttg aaaaaatact ttgaaagtga gctggctact ggtagtgcac tttactcgga
    15721 aggagtttat gcagtcgcat tggaaccttc aacaggtgcg gtgcttgcca tgtctggata
    15781 cagtcatgaa aaaggagccg gtagcataac agaagatgcc cttggtacta ttacaagtgt
    15841 tttcacacca ggttctatcg tcaaaggagc gacaatcagc tctggttggg aaaatggagt
    15901 tatcagtggc aatcaagtgc agttagacga accaatctat tttgcaggtt cagcaccaat
    15961 tacatcttgg tatggggcct atggtagttt taatattgat gcgacagagg ctctagaata
    16021 ttcttctaac gtctacatgg ttaagattgc tttgggacta cttggtcaga cctactcagc
    16081 taacatgtat ttgaatgatg gcgatacatt gacaaatgcc atgactaagc ttcgttcgac
    16141 ttttgcagag tatggtttag gtgcttcaac aggtattgac cttccactcg aatctacagg
    16201 atttttaccg gaagagtatt cgacagccaa ttttattacc aatgcttttg gacagtttga
    16261 taactataca ccgatgcaga tggcacagta tgcagcgacc attgcgaata acgggacacg
    16321 gatttctcca cacctcgttg agggaattta tgggaataat gcccaaggtg gtttggggga
    16381 attaattgaa accgtatctg gtaaggagat gaatcaggtt aatatttccg ctgaggatat
    16441 gtctcttctt cgtcaaggtt tctaccaagt tgtcaatggt agtgggcgct ttaatacagg
    16501 tagtgctatc ggtcgtggtg cggctgtgac catcagtgcc aagactggta cagccgaaac
    16561 ctacacaacg acagcttctg gtgatgtggt tactgcggtc aataccaacg ttgttgctta
    16621 tgcgcctagt gataatcctc aaattgcagt agcagttgtc ctaccaaatt tgaccaatca
    16681 aaattcaaca actactaaga caattactca agaaattatc aatctatatc aatcacttca
    16741 cccaatgaat taaggaggct tatgctttac cctacaccta ttgccaagtt aattgatagc
    16801 tattctaaat tgccaggtat cggtataaaa acggctaccc gtttagcttt ttataccatt
    16861 ggcatggagg atgatgtcgt taatgagttt gcaaaaaatt tattggcagc caagcgagac
    16921 ctatcttatt gctctatttg tggtaatttg acagatcagg acccctgtgc catttgccag
    16981 gactcaacgc gagaccaatc tactattttg attgtagagg atagtcgaga tgttacagcc
    17041 ttggaaaata ttcaagaata ccatggtctt tatcatgtct tgcatggctt gatttctccc
    17101 atgaatggta tcggaccaga tgatattaat ttgaaaaccc tcctcactcg cctgatggac
    17161 aatgaggtta cagaagttat tgtggcgacc aatgcaacag cagatggaga ggctacatct
    17221 atgtatatct cacgtgtcct caaacctgcg ggaatcaaag tgaccagact agctcgtggc
    17281 ttagcagtag gcagtgatat tgaatatgca gatgaagtga ccctgttgag ggctattgaa
    17341 aaccgcacag agctatagca gttctgtagt tagatacgtt cctaatttta agataggata
    17401 gatatgattt gttaaattat gctatactaa gagtaatagc gttgatacag gaaggaaaat
    17461 atatgtcaaa agaaaccttg attttactat atggtgggcg ttctgcagag cgtgaggtat
    17521 ctgttctgtc tgccgaaagc gtcatgcgtg ccatcaatta tgataaattt tttgtcaaaa
    17581 cctatttcat tagccagaca ggggacttta ttaagacgca agagttttcg caaacaccgt
    17641 ctgccgatga aaaactcatg acaaatgcga cgattgttga agagcaaaaa atccgtccga
    17701 gcgatattta tgaagaaaag gcagttgttt tccctgttct tcatgggcca atgggagaag
    17761 acggatctat tcaaggcttc ttggaagtgc ttcgtatgcc ttatgttggc actaatatct
    17821 tgtcttcaag cgttgctatg gacaaaatca ctaccaagcg cgtacttgaa tcagcaggaa
    17881 tctcccaagt accttatgtg gcagttatcg agggtgaaaa tatcgacgat aagatcgcag
    17941 agattgaagg aaaattgact tatccggtct ttgtaaaacc agccaatatg gggtcaagtg
    18001 tcggtatctc aaaagctgag gatttagctg gcttgcgtaa ggcacttgat ctggccttca
    18061 agtatgatag ccgtgtcctc gttgagcagg gagttaatgc gcgtgagatt gaggtcggtc
    18121 ttttgggaaa tgctgacgtg aaaaccaccc ttcctggcga agtagtcaaa gacgttgctt
    18181 tttatgacta cgatgccaaa tacatcgaca acaagattac tatggaaatc ccagctaaga
    18241 ttgatgagtc tatcatggat atcatgcgtg ataatgctgc caaggccttc cgtgctttcg
    18301 gtggctgtgg tctttctcgc tgtgatttct tcctaacaga agatggcgac attttcttaa
    18361 atgagttgaa taccatgcca ggatttaccc agtggtctat gtatccactc ctttgggata
    18421 atatgggctt ggcttaccca gacttgattg aagagcttgt tcgtttggct aaggaaatgt
    18481 ttgaaaaacg ggaaagtcac ctcatttaat tattatcaag attggaatca ttccaatctt
    18541 ttttcttgca aaggtttgca aaaaggaata aaatggaaca agttctggag gtgtttatgt
    18601 atcaaacgac agtaaaaggg gaaggtctgt ttcaggctct ttctcaaggt tatggcgaac
    18661 cggttcgtac ctttgggtta acggagaagg gtgagactcc ggttagtcta gttaatattg
    18721 gcttagctgc ctgtatcacc atgtgtgtgc agggctacta tgccagccaa gaaggcaata
    18781 aaaccatgcc tgttcaggtg gagagtcgct tggacaatca gcgttttgag gtgttgattg
    18841 gaattggtga gtcggtttcg gcgcaaaagc agaaagagat tcttgcctat gttgaaaaga
    18901 aatgtaaagt cacagctctt ttgaaggaag acttgcagtt tgagacaagt ttttttgtct
    18961 tagaaagtgt ggagtagatg tttttagcaa ttgaagaaat gcggcagaat aagctgcgtt
    19021 atggtttaat attaggacta ctggtcctga tttcctatct ggtctttttc ctgactggtt
    19081 tggcttacgg actcatgcag gaaaatagaa cagcagttga taaatggcag gcagactatg
    19141 tcctgcttaa ttcagaaagc aatcgcctga ttacagcatc aaaaattgat acagacttgc
    19201 ttgaccaggt ggacgctagt aacaaagcct tgattcgcca acaagctgga gttgcctatg
    19261 tggatgaaaa tgccacctca gacgaaaaag aaaaggtaaa catctttgct gttgaatcag
    19321 atagttttat cgtcccaaat attgtagaag gtcgtttgta tgaaaaaacg ggagaagtcc
    19381 ttgtagacaa aaccttgtca gaagtagaag gttttgggct aggtgatcaa atctatctat
    19441 caggttctga tgaaaaagtg accattgtag gttatacaga caatgcttat tttggagtga
    19501 cgcctgttat atatatggac tttacggcat ttgcagaatt gatgcaggct gatcaacaac
    19561 aagctgcaac gagtaatctt gccagtgcca ttgtcgttcg tggagatgtc gcttctctac
    19621 caaatgagct ggaaaaagtt gctattgcag actttattga aaatctgcca ggatataagg
    19681 cacaaaatat gacctttggc tttatgatag gattcttgat tgtcatctca gccattgtta
    19741 ttggtatttt tatctttgtt ttgacaacac agaaatctcc gatttttggc ttgatgaaaa
    19801 ttcaaggtct ttcaaatggc tatatttcag gttctgtatt ggctcaaacc ttcctacttg
    19861 ctggtgtggg aacagtcctt ggattggctg ggacttattt gtcatccctt gtcttgccaa
    19921 gtgctgttcc atttgagaat aactggacct tctatattgt aatcggccta gcactggttg
    19981 tatttgccct attgggtgct agtttctctg tacgttctat ctttaaggta gatccattac
    20041 gaaatctatc ttaggaggtt gttatgaaaa cacttatttt tgaaaatatt agtaagacct
    20101 tccaagatgg cagccaaacc attacggctc ttaaaccaac aaattttagc attgaagaag
    20161 gcgaatttgt tgccataatt ggaccatctg gttctgggaa gtcaacattt ttgaccttgg
    20221 caggtggttt acaaacaccg acaacgggtc gcgttttaat caatcaatcg gactattctg
    20281 atttacctga gaaaaaacgt gccaaattgc gctataagga cattggtttt gtcttgcagg
    20341 cttccaattt gattccattt ttaacagtcg aaaaacaatt aaccttggtt gataaggtca
    20401 ataagaaagc tgaaccgagc aagcgaaacg aactcctatc tgaattgggt gtggaccatc
    20461 tcaaaaacaa gttccctaag gatttgtcag gcggagaacg gcagcgtgta gctattgccc
    20521 gtgcacttta taacgatcca gctttgattc tagctgatga accaacagcc agtctagaca
    20581 gtaatcgcgc ttttgaagtg gttgagttat tagctaagga agccaaagaa cgaaacaaat
    20641 ccatcatcat ggtaacacat gaccagcgca tgattgaaaa aagcgacaag gtatatgaga
    20701 tgaaagatgg tgtcctcact caagttcgat aaaaaatggt ataatagaaa gagcgaaagc
    20761 tcttttttag tttattggag aaaacaatga aattaaccct acacgaagtc gctcaggtct
    20821 tgggcgcaaa aaatgatatt agtctttatc ctgatacggc actcaacaag ttggagttcg
    20881 acagtcgcct gattacagaa ggggatcttt ttgttcccct caaaggtgcg cgtgacggtc
    20941 atgactttat tcctgttgct tttgaaaatg gctgcgcagt aaccctgtct gaagtcagcc
    21001 ttgatgttcc gtacattcta gtagaggact gcctggctgc gcttcaacaa ttagcggcct
    21061 actacctaga gaaaacaggg gtagaagtca ttgccgtgac aggatcaaac ggtaaaacga
    21121 ccaccaagga catgattcac gatgttctgg cgacgactta caagacttac aagacacaag
    21181 gcaactacaa caacgaaatc ggtctgccat acaccgtcct ccacatgcct gatgatacag
    21241 aaaaactggt tttggaaatg ggccaagacc acttgggtga cattcatctc ctatctgaaa
    21301 ttgccaaacc aagtctgtcg gttattactc tatttggcga agcccatttg gaatttttcg
    21361 gctcacgaga agagattgcc aaaggaaaat tacagattgc ggacggcatg gcagcgggaa
    21421 ataaattgct gctccctgca gatccgattg cggataagct cttgccgagc catgtagacc
    21481 tcattcgctt cggtgagcag ggagatttgc aggtgactag tctggtcgag tcaaaagata
    21541 gcctgacctt caccgtcaac tttatggacg gcgacatttt ccttccagta acgggtaagt
    21601 acaatgccac taatgctatg gtagcaagct acgtaggtaa gactttgggt gtgccagatg
    21661 aggcaatcaa gtcagcccta gctggtctga acttgacccg caatcgtacc gagtggaaaa
    21721 aagcagcaaa cggagccgat attctcagtg atgtctacaa tgccaaccca acggccatgc
    21781 gcttgatttt ggaaaccttc tctagcattc cagccaatga aggcggcaag aaaatagctg
    21841 ttttggcaga tatgaaagaa ctggggagcc agtccgtcga tctccacaat cagatgattt
    21901 tgagcctgtt gcctgatgtg ctagacaccg tcattttcta cggtcaggat attgaaggtc
    21961 tggcccagtt ggctagtcag atgttcccaa taggaaaagt ctatttcttt aggaaaaatg
    22021 cagagcagga ccaatttgaa gatctggtta aacaggttag ggaaagtcta ggaaaagagg
    22081 accaaatcct ctttaaaggc tctaattcca tgaatcttgg gaaagtagtg gaagcaatag
    22141 aaaagtagag aggcgagacc tctctttttt agcaagtctt gtcaaaacat cgaaaagttg
    22201 atatactaac ttttggggaa agagaggaat ttaataagat gagtgatttt tttagcaatc
    22261 agatttctga agcgccgaac ctggacgtgt tggaacatct gtttctcatg attatttggt
    22321 ttttcatgct ttatctttgt tttcgttgtt atcaaaaaag atggtttcat attgtttttt
    22381 ggtggcttca atttatacag attgtctccc tctatacttg gtatattgtg gctgattttc
    22441 ctctttctga gagtcttcct ttttatcatt gtcgcttggc tatgctcttt attctttggg
    22501 cgaagcctgg acctttgaaa cgttattttg cctatctggg tctttttgga tcgctttcag
    22561 cctttattca tccggtattt gacccgtttg cttttccaca ccttaccttc tttacttttg
    22621 ttattggtca ctacgccttg acggttaatt gtatgttgta cctgttatct gatttagaag
    22681 gggagatgtt gaagggaaaa gaggtagtga aatatacgct tattatgaat atgctgattc
    22741 taggagtagc tcttttaact ggcggaaatt atggattttt gagacaggca cctttagtga
    22801 atactaataa tcttcctttg aatttcttct tggtaacttg cctactctgt ttctctattt
    22861 tgagtataca agctatgtta attgcctatt tgaaaaaaga aagcaaacaa atggattcgt
    22921 agatattgaa aaaataagac ttgaaattag tttttcaagt cttattttta gttatgtgac
    22981 ttttagtccg tcacgcctcg taaggtgcgt
//
LOCUS       DATUCG010000303        11382 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.guided.082, whole genome shotgun sequence.
ACCESSION   DATUCG010000303 DATUCG010000000
VERSION     DATUCG010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11382
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.guided.082"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            <1..344
                     /locus_tag="VCA20_002521"
     CDS             <1..344
                     /locus_tag="VCA20_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1836198.1"
                     /translation="IGTPKSKEEGFDVYNELKDLLVDRGIPKEEIAFVHDANTDEKKN
                     SLSRKVNSGEVRILMASTEKGGTGLNVQSRMKAVHHLDVPWRPSDVGRILRTFKIKKN
                     VEVTDNGKDNF"
     gene            325..699
                     /locus_tag="VCA20_002522"
     CDS             325..699
                     /locus_tag="VCA20_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001129922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnpV protein"
                     /protein_id="HEP1836199.1"
                     /translation="MAKTIFEEMGGKYERQGDYLIPCLTVPAEEEQPIGIWGQRHLDY
                     LKHHCKVTYTNLLTSGRLNAYLADIDRQAQERFERLIEGMKQAQGITEQLKAENALEW
                     TGCLNNIRACAREIVEKEIIFA"
     gene            1066..2985
                     /gene="tet(O)"
                     /locus_tag="VCA20_002523"
     CDS             1066..2985
                     /gene="tet(O)"
                     /locus_tag="VCA20_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032490535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(O)"
                     /protein_id="HEP1836200.1"
                     /translation="MKIINLGILAHVDAGKTTLTESLLYTSGAIAEPGSVDKGTTRTD
                     TMNLERQRGITIQTAVTSFQWEDVKVNIIDTPGHMDFLAEVYRSLSVLDGAVLLVSAK
                     DGIQAQTRILFHALQTMKIPTIFFINKIDQEGIDLPMVYQEMKAKLSSEIIVKQKVGQ
                     HPHINVTDNDDMEQWDAVIMGNDELLEKYMSGKPFKMSELEQEENRRFQNGTLFPVYH
                     GSAKNNLGIRQLIEVIASKFYSSTPEGQSELCGQVFKIEYSEKRRRFVYVRIYSGTLH
                     LRDVIKISEKEKIKITEMCVPTNGELYSSDTACSGDIVILPNDVLQLNSILGNEMLLP
                     QRKFIENPLPMLQTTIAVKKSEQREILLGALTEISDGDPLLKYYVDTTTHEIILSFLG
                     NVQMEVICAILEEKYHVEAEIKEPTVIYMERPLRKAEYTIHIEVPPNPFWASVGLSIE
                     PLPIGSGVQYESRVSLGYLNQSFQNAVMEGVLYGCEQGLYGWKVTDCKICFEYGLYYS
                     PVSTPADFRLLSPIVLEQALKKAGTELLEPYLHFEIYAPQEYLSRAYHDAPRYCADIV
                     STQVKNDEVILKGEIPARCIQEYRNDLTYFTNGQGVCLTELKGYQPAIGKFICQPRRP
                     NSRIDKVRHMFHKLA"
     gene            3037..3210
                     /locus_tag="VCA20_002524"
     CDS             3037..3210
                     /locus_tag="VCA20_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002779755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEP1836201.1"
                     /translation="MMKCEWILCPVCGSKTRNKIRKDTVLENYPLYCPKCRQESLIKV
                     DNLKITVIKEPDA"
     gene            3700..4209
                     /locus_tag="VCA20_002525"
     CDS             3700..4209
                     /locus_tag="VCA20_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEP1836202.1"
                     /translation="MKYAPRKVYIKESGGYVELSYTDFCRRRQADKGYMDKLFIPVQG
                     CLLEVVREQYADFYRDRERWRYLQKLDARNSLLSLDGFTDSEGNPLDFIADEAADIAE
                     TVVNAVMVDRLKAALPLLSDSEQELIQAIFFDGLSEREVGARLGITQSVVNKRKARIL
                     RKLRKIIEN"
     gene            4304..4729
                     /locus_tag="VCA20_002526"
     CDS             4304..4729
                     /locus_tag="VCA20_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEP1836203.1"
                     /translation="MAYNHGREDRKWRIWKEAEEKLLRECGVDEVTIEQIRIADRADF
                     NSNRRFYRWTNDVAEYLEDMADRERQAEVNTVAELLDEIESENLYQVLVTVDGRTLKI
                     VLLKMQGYSTKEIAPLVHLTTGAIYARLDHLRKKLRKIL"
     gene            4813..5286
                     /locus_tag="VCA20_002527"
     CDS             4813..5286
                     /locus_tag="VCA20_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEP1836204.1"
                     /translation="MAYKAKAYTLREESTESGIRYFISFKDGQGEHHELEVSEQLFFE
                     FRQMERRNRNLLQWDERHREFSEVWDETLNRRALKLPKSIEEQMIEAERAELLCKAVD
                     RLPEIQRRRFLLYYEYEFNFYQIAAMEHCTASAIQKSVAVAKKKVKAEMRKYLQP"
     gene            5440..6471
                     /locus_tag="VCA20_002528"
     CDS             5440..6471
                     /locus_tag="VCA20_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002596255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEP1836205.1"
                     /translation="MQKLQTVNAETLLYEPLEKPSFVVDSLIPTGLSLFCGSQKIGKS
                     WLMLKLCLCVSQGIPLWDMTTMEGDVLYLCLEDTFCRIQDRLFRLTDEASGRLHFAVA
                     SCKLSDGLIVQLEDYLKDYPDSRLIVIDTLQKVRTASKDNAYASDYGDISLIKDFADR
                     HSLAVIVVHHIRKQNDSDVFNKVSGTTGLTGSADATFVLEKEKRASDTAKLYVTGRDT
                     PYQEYTLRFRDCSWELVERKTQEQLAKETIPDVLFRLVDFMRDKEEWAGTATELLAAM
                     GETETIPTVITKWLNEYHTTFLSENRICYQYSRRKDGRQIALARRAGDSGDGGDSDIG
                     IPPVTVIDA"
     gene            6717..6983
                     /locus_tag="VCA20_002529"
     CDS             6717..6983
                     /locus_tag="VCA20_002529"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1836206.1"
                     /translation="MPDVPSGLPYGRSYALLSYALRLRIKERRHDGYSSCEGVSQKHR
                     HHRHDRHAGDKGDSYGNRQGASQNCRHHRHRCHPKGSYPPKKGR"
     gene            6990..7955
                     /locus_tag="VCA20_002530"
     CDS             6990..7955
                     /locus_tag="VCA20_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HEP1836207.1"
                     /translation="MPYAILRFQKRKAGGVAACERHNERKKEAYKSNPDIDMERSKNN
                     YHLVAPPKYTYKKEINRKVAEAGCRTRKDSVMMVETLITASPEFMNQLPPEEQKAYFT
                     MALDFISERVGEQNILSAVVHMDERTPHMHLCFVPITPDNKLSAKTILGNQKSLSEWQ
                     TAYHERMSSRWNQLERGQSSMETKRKHVPTWLYKLGGRLDKQYGEIVSALSDINAFNA
                     GKKRDKALELVAAWLPEVEKFSKEIGRQQAYIDSLKEQIGQEADYAGRMRDEKYEQEL
                     KVQKANQRIFELQRTNEQMGRLLSKIPPEVLEELQRTGRNKSRER"
     gene            7963..8817
                     /locus_tag="VCA20_002531"
     CDS             7963..8817
                     /locus_tag="VCA20_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002596257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEP1836208.1"
                     /translation="MKKQDFKVLKTKDLYPFPDNPFHVVEDETLSELAESIKEFGIVT
                     PIITRPKEDGNGYEVIAGQRRVRASELAGINTVPAFVLPLDRDRAIITLVDSNLQREN
                     ILPSERAFAYRMKSEAMKRQGFRTDLTSSQVVTKLRTDDKVAQGFGVGRMTVQRFIRL
                     TELIPPILQMVDEGKIALTPAVELSFLKKDEQENLFATMESEEATPSLSQAQRMKSMS
                     QSGQLDMDMIFSIMTEEKGNQKETLKINTSKLKKYFPKDTTPKQMEETIIRLLERELQ
                     RKRSRDSR"
     gene            8894..9124
                     /locus_tag="VCA20_002532"
     CDS             8894..9124
                     /locus_tag="VCA20_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1836209.1"
                     /translation="MKEIQYEIVKEIAVLSASDSGYTKEINLISWNGREPKYDIRSFS
                     PNREKCGKGITLNADEAAALLEALQKEVNSGD"
     gene            9195..9386
                     /locus_tag="VCA20_002533"
     CDS             9195..9386
                     /locus_tag="VCA20_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HEP1836210.1"
                     /translation="MGEDRKTTGRRKRIVPKGQVQMIISREYIGTQTVAEAFVPIISE
                     DIRKKIAEGDTFDNEGLSA"
     gene            9440..11323
                     /locus_tag="VCA20_002534"
     CDS             9440..11323
                     /locus_tag="VCA20_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEP1836211.1"
                     /translation="MAGIRTENKIYEVGIYCRLSKDDGTDNESASIATQKSILTDYVK
                     RQGWHLAKTYVDDGYSGTNFQRPSFQNMIKDIENGLINCVITKDLSRLGRNYLDCGLY
                     LEVFFPEHNVRYIAVNDGVDTLNKSAMDITPFRNILNEMYSADVSVKIKSTYRARFQQ
                     GKFMGTTAPYGYVKDPADHNHLLIDDKVAHVVREIFDLALAGNGIAKIRKHINKQHIL
                     RPAAYAVEQGATGYERYFEDNEENRYIWSENSVRGILRSPIYAGNLAGYKRIAANMKS
                     KKRPSKLPEEWEVIPDTHEGIVTQEEFDTVQQLMTSRRREQNAGGFENIFSGVIKCAD
                     CGYAMRAASANRRKRPDIIDCVQYTCNNYGRYGNVMCTAHSIEARDLFNAVLADINRF
                     ADMAVNDEKAVRAIERRLTETDQSRAKSLEKEKKKLNKRLAELDRLFSSLYEDKVMER
                     ITERNFEMMSGKYQKEQLEIEARLKEVTETLNDSYEKSQGVRDFLSLIRNYQGLKELD
                     ATIINALIDKILVSEREKLADGTVRQEIKIYYKFIGFVGELHITPTKRWTALKPKNCT
                     VCGVEYVPSSGISKYCPACAKRIQREKSNESKRRSRERNRRACIELSAKNDRLILSSE
                     MDD"
BASE COUNT         3457 a         2314 c         2981 g         2630 t
ORIGIN      
        1 atattggaac ccctaaaagt aaggaagaag ggtttgatgt ttacaatgaa cttaaggact
       61 tgttggtcga tcgaggaatt ccaaaagaag aaattgcctt tgtccatgac gccaatactg
      121 atgaaaagaa aaactcattg tctagaaagg tcaatagcgg agaagtgcgg attctcatgg
      181 cttcgacgga aaaaggtgga acgggtttaa acgtgcaatc acgcatgaaa gctgtccacc
      241 acttagatgt tccctggcgt ccctctgatg taggacggat tttgcggaca ttcaaaataa
      301 aaaagaatgt ggaggtaaca gacaatggca aagacaattt ttgaggaaat gggcggcaaa
      361 tacgaaaggc aaggcgatta tttaataccg tgcttaactg tacccgccga agaagaacag
      421 ccgataggca tctggggaca gcggcatttg gattatctga agcatcactg caaagttaca
      481 tacaccaatc ttcttacaag cggcagactt aacgcttacc ttgccgacat tgacagacag
      541 gcacaggaac gctttgaaag gctcatagag ggtatgaaac aggcacaggg cataacggaa
      601 cagctaaagg cagaaaacgc cttagaatgg acaggatgcc tcaataacat aagggcttgt
      661 gcgagggaga ttgtggaaaa ggaaattatt tttgcataaa cagatgatta gtggcagggg
      721 gaaatcctgc cgctttttct gctttagttt gtcagcttga caaataaagg gttaaggaat
      781 ataattagat tcagtattat acaaggagtt aataaatatg cggcaaggta ttcttaaata
      841 aactgtcaat ttgatagtgg gaacaaaaag tagcagtccc gtttcacttt taatatgggg
      901 cttagttttt tgtacccagt ttaagaatac ttttatcatg taattttata tgcccgaaaa
      961 catataagtg ttttggggct attggagtta tttacccagt gataggagta tttatcactg
     1021 ggtattttta tgcccttttt tgggtgttga taggaggaaa atcacatgaa aataattaac
     1081 ttaggcattc tggctcacgt tgacgcagga aagacaacat taacggagag tttattgtat
     1141 accagtggtg caattgcaga accagggagc gtagataaag gcacaacaag gacagataca
     1201 atgaatttgg agcgtcaaag gggaatcact atccagacag cagtgacatc ttttcagtgg
     1261 gaggatgtaa aagtcaacat tatagatacg ccaggccata tggatttttt ggcggaagta
     1321 taccgttctt tatccgtatt agacggagca gtattattag tttctgcaaa ggatggcata
     1381 caggcacaga cccgtatact gtttcatgca ctacagacaa tgaagattcc gacaattttt
     1441 ttcatcaata aaattgacca agaggggatt gatttgccaa tggtatatca agaaatgaaa
     1501 gcaaagcttt cttcggaaat tatagtgaag caaaaggttg ggcagcatcc ccatataaat
     1561 gtaacggaca atgacgatat ggaacagtgg gatgcggtaa ttatgggaaa cgatgaacta
     1621 ttagagaaat atatgtcagg gaaaccgttt aaaatgtcag aactggaaca ggaagaaaac
     1681 aggagattcc aaaacggaac gttatttccc gtttatcacg gaagtgctaa aaacaatctg
     1741 gggattcggc agcttataga agtgattgcc agtaagtttt attcatcaac gcctgaaggt
     1801 caatctgaac tatgcgggca ggtttttaag attgaatatt cagagaaaag gcggcgtttt
     1861 gtttatgtgc gtatatatag cggaacattg catttgaggg atgttattaa aatatctgaa
     1921 aaagagaaaa taaaaatcac agagatgtgt gttccgacaa acggtgaatt atattcatcc
     1981 gatacagcct gctctggtga tattgtaatt ttaccaaatg atgttttgca gctaaacagt
     2041 attttgggga acgaaatgct gttgccgcag agaaaattta ttgaaaatcc tctccctatg
     2101 ctccaaacaa cgattgcagt aaagaaatct gaacagcggg aaatattgct tggggcactt
     2161 acagaaattt cagatggcga ccctctttta aaatattatg tggatactac aacgcatgag
     2221 attatacttt cttttttggg gaatgtgcag atggaagtca tttgtgccat ccttgaggaa
     2281 aaataccatg tggaggcaga aataaaagag cctactgtta tatatatgga aagaccgctt
     2341 agaaaagcag aatataccat ccacatagaa gtcccgccaa atcctttctg ggcttctgtc
     2401 gggttgtcca tagagccgct ccctattgga agcggagtgc agtatgaaag cagagtttca
     2461 cttggatatt taaaccaatc gttccaaaat gcggttatgg agggggttct ttatggctgc
     2521 gagcaggggc tgtatggatg gaaagtgaca gactgtaaaa tctgttttga atatggattg
     2581 tattatagtc ctgtaagtac ccccgcagac tttcggctgc tttcccctat cgtattggag
     2641 caggctttaa aaaaagcagg gacagaacta ttagagccat atctccactt tgaaatttat
     2701 gcaccgcagg aatatctctc acgggcgtat catgatgccc caaggtattg tgcagatatt
     2761 gtaagtactc aggtaaagaa tgacgaggtc attctgaaag gagaaatccc tgccagatgt
     2821 attcaagaat acaggaacga tttaacttat ttcacaaatg ggcagggagt ctgcttgaca
     2881 gagttaaaag gataccagcc agctattggt aaatttattt gccaaccccg ccgcccgaat
     2941 agccgtatag ataaggttcg gcatatgttc cacaagttag cttaacagct tgcaaaagtc
     3001 atataaaatg agatttgaaa ggattagaga ctaattatga tgaaatgcga atggatattg
     3061 tgtcctgttt gtgggagcaa aacccgtaat aaaattagga aggacactgt tttggagaat
     3121 tatccccttt attgtccaaa atgcagacaa gaaagcttga ttaaagttga caacttgaag
     3181 ataactgtca tcaaagagcc agacgcttaa gacgcagagc cgatgaaatt gtggaacaat
     3241 tcacgaatca tcggctcttt ttgtttcgta tttgaaagaa caagctcacc aaataaaaaa
     3301 aacgatattc gggtgggtta ttttgttata ccctaaatta ccctctgaat ttgtttttaa
     3361 atttggaggg atttttttat gttctttttt cgggcggtta ttcatctgct catacgaagt
     3421 aaaatttatc aatacatagc atatcagaga atggcaggaa accagttaaa aaaatttctg
     3481 ccagtgcagc ggtacttctc accttgaaag tggaagtaca atctccatac aacagaattt
     3541 gtatttccca taaccgtgaa ggtcacagag cctttgcggt ttttcttttg tcgttttttg
     3601 ttggaaagtg ccgcagggct gtttctggtg tgcgttgccg ctccccgcct ctccatctgg
     3661 atttttacat ttcaaaaatt cagattggag gtattagcgg tgaaatacgc accaagaaaa
     3721 gtatatatca aagaaagcgg cggctatgtg gaactgtcct atacggattt ctgccgccgc
     3781 aggcaagccg acaagggata tatggacaag ctgtttatcc ccgtccaagg ttgtctgctt
     3841 gaagtcgtaa gggagcaata tgcggacttc taccgtgaca gggagcggtg gcgttacctg
     3901 caaaaattag atgcgaggaa cagcctgcta tctctggacg gatttacgga cagcgagggg
     3961 aatcctctgg actttatcgc tgatgaagcg gcggacattg cggaaaccgt tgtcaatgct
     4021 gttatggtgg acaggctgaa agccgccctg cctttgttgt cggatagtga gcaggagttg
     4081 atacaggcaa tcttttttga cggactttcc gagcgtgaag tcggggcgag gttaggcata
     4141 acccagagcg ttgtgaacaa acgcaaagcc agaatcctaa gaaaactaag aaagataata
     4201 gaaaattaaa atttaaggcg ttcagccccc ttgttttttc ctttgggaaa atgagggggc
     4261 ttttctctgc cctctcaatc aattctgatt ggaggaagaa agaatggcat acaaccacgg
     4321 acgggaggac aggaaatggc gtatctggaa agaagcggag gaaaagctgc tgcgtgagtg
     4381 cggcgttgat gaagtgacca ttgagcagat acgcatagcg gacagggcag acttcaattc
     4441 caacaggcgg ttttaccgat ggacgaatga cgttgcggaa taccttgagg acatggcaga
     4501 cagggagcgg caagcggagg tgaatacagt tgcggagtta ctggacgaga ttgagagcga
     4561 aaatctctat caagtattag tcacggtgga cgggcgtacc ttgaaaatcg tcctgctgaa
     4621 aatgcagggg tattccacaa aggagattgc cccacttgtg catttgacga ctggtgccat
     4681 ctatgcgagg ttagaccatc tgcggaagaa gcttcggaaa attttgtaag cgtctaatac
     4741 atcccattat tctgcgggct actgggtggg gagtggaatc tccccgctta tttttggcag
     4801 gaggaaaacg ggatggcata taaagctaag gcgtacacgc ttcgggagga atccacggaa
     4861 agcggtataa ggtattttat cagttttaag gacgggcagg gggaacacca cgaactggaa
     4921 gtatccgagc agttattctt tgaatttcgg cagatggaac gcaggaacag gaatcttctc
     4981 caatgggacg agcggcacag ggagttttcg gaagtatggg acgaaacgct gaacaggcgg
     5041 gcgttaaagc tgcctaagag cattgaggaa caaatgattg aagcagaacg ggcggaactg
     5101 ctctgtaagg cggttgacag actgccagag atacaaaggc gacgtttcct gctctactac
     5161 gagtatgagt tcaattttta ccaaatcgcc gctatggagc attgcaccgc ttctgcaata
     5221 cagaaatccg ttgctgttgc aaagaagaaa gtaaaagcgg aaatgaggaa atatctccaa
     5281 ccatgaccga caccgcccga aaaagaaatc tgttttttat ttgtgtggga ttggcggtat
     5341 tacatactct cacttttttc gtgggagtaa atctattttt aaggaggtca acgcctatgt
     5401 gcaacaaaaa caagataact gcccgaaagg aggaaaccca tgcagaagtt acagacagtc
     5461 aacgccgaaa cgctccttta tgagccgctt gagaagccgt cctttgtggt ggacagcctt
     5521 atccccacag gcttgtcgct gttctgcggc tcacagaaga taggcaagag ctggctcatg
     5581 ctgaagctgt gcttatgcgt gtcgcaggga atccccttat gggacatgac gacaatggag
     5641 ggcgatgtgc tttacctctg ccttgaggac acgttctgcc gcatacagga caggctgttc
     5701 cgtttgacgg acgaagcgag cgggcggctt cattttgccg tggcaagctg caagctgtca
     5761 gacggtctta tcgtgcagct tgaggattat ctgaaagact atccagacag caggctcatt
     5821 gtcattgata ccttgcagaa agtccgtaca gcttcaaaag acaacgccta tgcaagcgac
     5881 tatggggaca tctccctaat caaagatttt gccgacaggc actctctggc ggtcattgtc
     5941 gtacaccaca tccgaaagca gaatgacagc gatgtgttca acaaggtgtc tgggacgaca
     6001 ggcttaacgg ggagtgcgga cgctaccttt gttctggaaa aggagaaacg ggcatctgac
     6061 accgccaagc tgtatgtaac gggcagggac acgccctatc aggaatacac gctccgtttc
     6121 cgtgattgca gttgggaact tgtggagagg aaaacgcagg agcagcttgc gaaagaaacg
     6181 ataccagatg tcctttttcg gttggtggat tttatgaggg ataaggaaga atgggcaggc
     6241 acggcaacgg agctgttagc cgctatgggg gaaacggaaa ccatacccac ggtgattacg
     6301 aaatggctga atgaatatca caccacattt ttaagcgaga accgtatctg ctaccagtac
     6361 agccgcagga aagacggcag gcagattgcc cttgcaagga gggcgggtga cagcggtgac
     6421 ggcggtgaca gcgatattgg gataccccct gttactgtca ttgacgctta aagccatgta
     6481 aagttggcgg ctctgccctg cgggtgacag cggtgacggc agtgacagtg attttaggat
     6541 accctgccgc tgtcatccct acgggagaac accccgcaaa cgcaaaatgc aggcgtggga
     6601 ttcacccgcc cttttcgggc gtgtaaaatc acccctgcgg tcagaaaaat ctctccgatt
     6661 tttccgactg cgtttacagg gtgtaacaca ctacactttg ccctgcaaag agccgtgtgc
     6721 cagacgttcc ctctggactc ccttatggca ggtcttacgc cctgctatcc tacgccttgc
     6781 ggcttcggat aaaagaacgg cggcatgacg gttacagctc ttgcgaggga gtatcccaaa
     6841 aacaccgtca ccatcgtcat gaccgtcatg caggggataa gggtgacagt tacggcaatc
     6901 ggcagggggc atcccaaaac tgccgtcacc accgtcaccg ctgtcaccca aagggcagtt
     6961 acccgccgaa gaaaggaaga tgatggatta tgccctatgc aatcctgcgt ttccagaaac
     7021 gcaaagcggg cggcgttgcg gcttgcgaac gccacaacga gcggaagaaa gaagcctaca
     7081 aaagcaaccc agatatcgac atggaacgct ctaaaaataa ctatcatctt gtcgcaccgc
     7141 caaagtacac ctataagaaa gagattaacc gaaaggtagc cgaagcaggg tgcaggacaa
     7201 ggaaagacag cgtgatgatg gtggaaacgc tcatcacggc ttcaccagaa tttatgaacc
     7261 agttaccgcc cgaagaacag aaagcgtatt ttacgatggc tctggatttc atttcggagc
     7321 gtgtcgggga gcagaatatc ctctccgcag tcgtccacat ggacgagaga acgccccata
     7381 tgcacctctg ctttgtgccg attacaccag acaataagct gtccgccaaa acaattttag
     7441 gcaatcaaaa gagcctgtcg gagtggcaga ccgcctacca tgagcggatg tcctcacgtt
     7501 ggaatcagct tgagagaggt caatcttcga tggaaaccaa gcggaaacac gtccccacat
     7561 ggctctataa gttgggcggc aggcttgata agcagtatgg ggaaatcgtg tctgccctgt
     7621 ccgacatcaa cgcctttaac gcagggaaaa agcgtgacaa ggctctggaa ctggttgccg
     7681 catggctgcc cgaagtggag aaattctcta aggaaatcgg cagacagcag gcgtatatcg
     7741 acagcttaaa ggagcaaatc gggcaggaag ccgactatgc agggcgtatg cgtgatgaaa
     7801 agtacgagca ggaattaaag gtgcagaaag ccaaccagag gatatttgaa ttgcagagaa
     7861 ccaacgagca gatggggcgg ctgctctcaa agataccgcc agaagtgttg gaagaattac
     7921 agagaacagg cagaaacaaa tcaagagaaa ggtaaaaagt gaatgaagaa acaggatttt
     7981 aaggtgttaa agaccaaaga tttatacccg tttcccgaca atccgtttca tgttgtggag
     8041 gatgaaacgc tgtcagagtt agcggaaagc atcaaggaat ttggcattgt cacgccgata
     8101 atcacacgcc cgaaagagga cgggaacggt tatgaagtga ttgcggggca gcggcgtgtc
     8161 cgtgcgtccg aacttgcagg tataaatacc gtgcctgcat ttgtcctgcc cttagaccgt
     8221 gaccgagcca tcatcaccct tgtagacagt aatttacagc gtgaaaatat cctgccatcg
     8281 gagcgggcgt ttgcctacag gatgaaatcc gaagccatga agcggcaggg tttccgcaca
     8341 gacttaacct cgtcacaagt tgtgacgaag ttgcggacgg acgacaaagt ggcacagggc
     8401 ttcggcgtgg gcaggatgac cgtccagcgt tttatccgcc tgacggaact gataccgccg
     8461 attttgcaga tggtggacga ggggaaaatc gccctcacac ctgcggtgga actgtccttt
     8521 ttgaagaaag acgagcagga aaatctcttt gccacgatgg agagcgaaga agcaacgccc
     8581 tcactctcac aggcacagcg gatgaaaagc atgagccaga gcgggcagct tgacatggat
     8641 atgatatttt ccattatgac ggaggaaaag ggaaaccaga aagaaacctt gaaaatcaac
     8701 acaagcaaac tgaaaaaata ctttccgaag gacacaacgc cgaagcagat ggaggaaacc
     8761 atcatccgac ttctggaacg tgagttgcag agaaaacgga gcagggacag ccgctaacct
     8821 tcttttttcg ggaagtaaat gcaggaattt ggaacagaag aaagacagaa ttttaaggag
     8881 aatgaggtaa ggaatgaaag aaatccagta tgagattgta aaggaaatcg ccgtattgtc
     8941 tgcgagtgac agcggctaca caaaggaaat caatcttatt tcatggaacg ggagagagcc
     9001 gaaatatgac atccgcagct tttccccgaa ccgtgagaag tgcggaaagg gtatcacgtt
     9061 gaacgctgat gaagcagcgg cactccttga agcattacag aaagaagtaa acagcgggga
     9121 ttgatggtat ctgattgaca gggcggggcg tttccagacg ttctcctgcc ctgtctggaa
     9181 aggaagattt aagtatgggc gaggatagga aaacaactgg aagaagaaag cgtattgtgc
     9241 caaaagggca agtacaaatg attatcagtc gagaatatat cggcacacag accgttgcag
     9301 aagcgtttgt cccgattatc tccgaggata ttcggaagaa gattgccgag ggcgacacct
     9361 tcgacaatga ggggctgtcc gcttagaatg tacgcaatgg aacatgaaaa cgttaggaca
     9421 gatatggagg ttttacagta tggcaggaat aagaacggag aacaagattt atgaagtcgg
     9481 catttactgc cgcttatcaa aggacgatgg cacggataac gagagtgcga gcattgcgac
     9541 acaaaaatcc atcctcacgg attatgtgaa aaggcaggga tggcatttag caaaaacgta
     9601 tgttgacgat ggctactctg gaacaaattt ccaaagacca agtttccaga atatgattaa
     9661 ggacattgaa aacgggctga taaactgcgt gattacaaaa gatttatccc gtctggggag
     9721 gaactatctt gattgtgggt tatatctgga agtctttttc ccagagcata acgtgaggta
     9781 tatagcggtc aatgacggcg tggacacctt aaacaaatcc gctatggaca tcacgccttt
     9841 ccgcaacatc ctaaacgaaa tgtattctgc cgatgtgtca gtcaagataa aatcgacgta
     9901 ccgtgcgagg tttcagcagg ggaaattcat gggaacaaca gccccttatg gctatgtcaa
     9961 agaccctgcc gaccacaacc atctgctgat agatgacaaa gttgcccatg tggtaaggga
    10021 gatattcgac cttgcgttag cgggcaacgg catcgccaaa atccgcaagc acatcaacaa
    10081 acagcatatc ctacgccctg ccgcttatgc ggtggagcaa ggggcaacag gctatgagcg
    10141 gtactttgag gataacgagg aaaaccgtta tatttggagc gagaacagcg tgaggggcat
    10201 tttaagaagc ccgatatatg cgggcaacct tgcaggctac aagcggattg cagccaacat
    10261 gaaaagcaag aaacgcccct ctaagctgcc cgaagaatgg gaagtgatac cagacaccca
    10321 tgaggggata gtcacgcagg aggaatttga caccgtacag cagcttatga cgagccgcag
    10381 gcgggagcag aacgcagggg gatttgagaa tatcttttcg ggcgttatca agtgtgcgga
    10441 ctgcggctat gcgatgcggg cggcgagtgc caacaggagg aaacgccccg acatcatcga
    10501 ctgcgtacaa tacacctgca ataattatgg cagatatggc aatgttatgt gtaccgcaca
    10561 cagcattgaa gcgagggact tattcaatgc cgtccttgcc gacatcaacc gctttgcgga
    10621 tatggcggtc aatgatgaaa aggcggtcag agccatagaa aggcggctca cggaaacaga
    10681 ccagagcagg gcgaaatcat tagagaaaga aaagaagaag ctgaacaaac gccttgcgga
    10741 gcttgacagg ctgttttcct ctctctatga ggacaaggta atggagcgta tcacggagcg
    10801 gaattttgag atgatgtcgg ggaaatacca gaaagagcag cttgaaattg aagcacggct
    10861 gaaagaggta acggaaacgc tcaatgacag ctatgagaaa tcgcagggcg tgagggactt
    10921 tctatcccta atccgcaact atcaggggct gaaagagttg gacgcaacca tcataaacgc
    10981 actcatagac aagatacttg tttcggaacg tgagaaactt gcggacggga cagtaaggca
    11041 ggaaatcaag atttactata aattcatcgg ctttgtcggt gaattacata tcacacccac
    11101 aaagcggtgg acggcgttaa agcctaaaaa ttgtacggtg tgcggtgttg aatacgtccc
    11161 cagctctgga atatcaaagt attgtcccgc ttgtgctaag aggatacaga gggagaaatc
    11221 aaacgagagc aaacgcagga gtagggagag gaaccgaagg gcatgtattg aactgtccgc
    11281 aaaaaatgac cgactgatat tgtccagcga aatggacgac tgattcgaca aggaaacatg
    11341 agccaggagg tagatattta tcactatatt actaaaggga gc
//
LOCUS       DATUCG010000304         3699 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Streptococcus suis isolate NK028
            SAMN38798994-rid20660163.guided.179, whole genome shotgun sequence.
ACCESSION   DATUCG010000304 DATUCG010000000
VERSION     DATUCG010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38798994
            Sequence Read Archive: SRR27195369
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040768.1
            Genome Coverage       :: 323x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 22:45:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,536
            CDSs (total)                      :: 2,487
            Genes (coding)                    :: 2,334
            CDSs (with protein)               :: 2,334
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 80 of 153
            Pseudo Genes (incomplete)         :: 63 of 153
            Pseudo Genes (internal stop)      :: 37 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3699
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38798994-rid20660163.guided.179"
                     /isolate="NK028"
                     /isolation_source="pig"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:1307"
                     /country="Czech Republic"
                     /collection_date="2018"
     gene            147..1874
                     /locus_tag="VCA20_002535"
     CDS             147..1874
                     /locus_tag="VCA20_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1836212.1"
                     /translation="MFRLIFDYIKRHKWLYLLVAVTLIIYDATLLLPTQIIQRMVDIL
                     TKNELTQAILVQEMTLLLLVTVLNYATAFIWHLKLFQASVNFKFDMQQRAFKKLVTMR
                     TPFYEKFRSGDVMTRFSTDVDGLMEMVGYGLMIVVYAGGMLAFIIPTMFFIDWKISLV
                     ALLPMLFMTLCIFFIGRKQDEAIDANREAVAQLNNEVLEVIEGIRVTRAYSKKANQKA
                     QFQARTKQLAQGGDRITSLQSLYNPLATVCLGLSTIFVLLMGAQAVKAGQLTLGQVIA
                     LQLYVGSLLEPFWTLADFILVYQTGKTSFEKLQELIETGDDLEADGSKEIAELSSISF
                     KNYSFSYPQAERASLQDINWTLKAGQTVGIVGKTGSGKTTLVRQFLRQYPIGRGNFFI
                     NHQSVLDFKRSSIEEKIGYVPQEHILFSRSVGENIALGKVASSSEEIEQAIATAAFSQ
                     DLKRMTDGLDTMIGERGVSISGGQKQRISIARAFLREPDLLILDDSLSAVDARTERQI
                     IQNIQKERAGKTNVIVTHRLSAVNHADWVLVLDEGRIVEKGRPADLLAQRGWYYEQYQ
                     RQQSQEGGE"
     gene            1874..3613
                     /locus_tag="VCA20_002536"
     CDS             1874..3613
                     /locus_tag="VCA20_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1836213.1"
                     /translation="MTILGFLLKQIGRVKWLFIVAVGFYLLASTMVRLAPLLIQQAID
                     GPITDLSKGLPFDEAVFLNQSAQYMGMIILGAIGFYLSMRLLMHCANRIAENLRNQAY
                     DVMQRLPISYFDDKPAGKIATRIVNDTETLRTQFYGTLVNAFNNIVRLLFTYGVLFYM
                     NRSLGWLMLLLIPLYIGIQFAYKKMTDKPMKDFYDARSDVNTQVNETMNGASLIQLFG
                     QEERIMKEFEVTADKMRRADNKIIWAQSLATWNLSGFLQNLVIAAILTVVGYQFLAGQ
                     DGVTAGKLFVYVNYIEGVFIALGALVQQFPNMLRSFETGKRLMALLEEEVEDDCDRIL
                     EVEQGQVVFEHVNFSYEEHRPVLKDITIRAEKGETVALVGHTGSGKSSIMNLLYRFYD
                     PQEGRVLIDEKNIRDYSRESLRSHMGIVLQDPYLFTGTIASNVSMNEEEADRTRIMQA
                     LEKVGAGPMLSRLEKGIDEPVVEKGAAFSSGERQLIAFARTLYSDPKILILDEATSHI
                     DTETEEIIQHAMEVVKEGRTTFIIAHRLSTIQNADQILVLDQGRIIEHGKHEELVARG
                     GVYAQMHEIQAKV"
BASE COUNT         1028 a          674 c          874 g         1123 t
ORIGIN      
        1 ttgtcaactt gccttgatga actccagttc tatcttcgac atcgttttct aggctacttt
       61 tgattttcat tgagtataac gtctatcaat tgttgctgat tgaataaagt ggcaaatgat
      121 aggttataga tttgtaaagg agatatatgt ttcgattaat ttttgactat atcaagcgtc
      181 ataagtggct ctatctattg gttgctgtga ccttgattat ttatgacgcc acacttttgc
      241 taccaacgca aatcattcag cggatggtcg atatactaac caagaatgaa ttaacacagg
      301 ccatattggt tcaggagatg accttattgt tattggtaac agtgctgaac tatgcaacag
      361 ccttcatttg gcatctgaaa ttatttcagg cctctgtcaa tttcaagttt gatatgcagc
      421 aacgtgcctt taaaaaatta gtgaccatgc ggactccatt ttatgaaaaa ttccgctcag
      481 gagatgtcat gactcgtttt tcaacggatg tggatggcct gatggagatg gtgggttacg
      541 gtcttatgat tgtcgtctat gcaggtggta tgttggcttt tatcattccc acgatgttct
      601 ttattgattg gaaaatttcg ctagtagctt tactaccgat gctgttcatg acactatgta
      661 tattttttat cgggagaaaa caagatgagg cgattgatgc caatagagaa gctgtagccc
      721 agctcaataa tgaagttttg gaggtcatcg aaggtattcg agtgactcgg gcttatagta
      781 aaaaagcaaa tcaaaaggct caatttcaag ctcgtaccaa gcaattggct caaggtgggg
      841 atcgcatcac ttccttgcaa tcattgtata atccattagc cacagtctgt ttgggattgt
      901 ctactatttt cgtacttctc atgggggcac aagccgtgaa agcagggcaa ctgaccttgg
      961 gacaagtaat tgcccttcaa ttgtatgtcg gttcgctttt agagccgttt tggacactag
     1021 cagatttcat ccttgtttat cagactggta agacatcttt cgaaaaactg caagagctga
     1081 ttgagacggg tgatgatttg gaggctgatg gttctaagga aattgcagag ctgtctagta
     1141 tttccttcaa aaattatagc tttagttatc cacaggcgga aagagccagc ctacaggaca
     1201 tcaattggac actgaaagca gggcaaactg taggtatcgt tgggaaaact ggttcgggaa
     1261 aaacgacctt ggttcgccaa ttcttacgcc agtatccgat tggtcgggga aattttttca
     1321 tcaatcatca atctgtttta gactttaaac gttctagtat agaggaaaaa attggctatg
     1381 ttccacaaga acacattttg ttttcaaggt ctgttggaga aaatattgcc cttgggaaag
     1441 tagctagtag ctctgaggag attgaacaag ctattgccac agcagctttt agtcaagatt
     1501 taaaacggat gactgatggt cttgatacca tgattggtga gcgtggtgtg tctatttctg
     1561 gcggtcagaa acaacggatt tccattgccc gtgctttcct gcgagagcca gatttgctaa
     1621 ttctagatga ttctttgtcg gcagtggatg ctcggacaga acgccagatt atccaaaata
     1681 ttcaaaaaga acgtgcaggc aaaaccaatg tcatcgtgac ccatcgtcta tctgctgtca
     1741 accatgcaga ctgggtacta gtcttggatg aggggcgtat cgttgagaaa gggcgtccag
     1801 ctgatttgct tgcccaaaga ggttggtact atgaacaata ccaacgacaa caaagccagg
     1861 aaggaggaga gtaatgacta ttttaggttt tttattaaaa caaatcggtc gtgttaagtg
     1921 gctatttata gtagctgtcg gcttctatct attggcttcg accatggttc gattggctcc
     1981 tctgctgatt cagcaggcga ttgatgggcc gattacggat ttgagcaagg gtttgccctt
     2041 tgatgaagct gttttcttaa accagtcagc tcaatacatg ggaatgatta ttctgggagc
     2101 tattggcttc tacctatcta tgcgtttact catgcattgc gccaatcgta ttgctgaaaa
     2161 tctacgtaat caagcttatg atgttatgca acggttgcct atttcttatt ttgatgataa
     2221 gcccgctgga aaaattgcaa ctagaattgt caatgataca gaaactctgc ggacacaatt
     2281 ttatgggacc ttagttaatg cctttaacaa tattgttcgt ttgctcttta catatggcgt
     2341 cctattttac atgaatagga gtcttggttg gctgatgtta ctgctaattc ctctatatat
     2401 tgggattcaa tttgcttata aaaaaatgac tgacaagccc atgaaggact tctatgatgc
     2461 cagaagtgat gtgaatactc aggtaaatga aaccatgaac ggtgctagtt taattcaact
     2521 ctttggacaa gaagagcgta tcatgaaaga atttgaagtg actgctgaca agatgcgtcg
     2581 ggcagataat aagattatct gggcccagtc tcttgccacg tggaatctga gtggttttct
     2641 gcaaaattta gtcattgctg cgattttaac ggtagtcggc tatcaatttc ttgcgggaca
     2701 ggatggagtt acagctggga aactctttgt ctatgtcaac tatatcgaag gggtcttcat
     2761 tgctttggga gcgctggttc aacagttccc aaatatgctt cgatcgtttg aaacaggtaa
     2821 gcgtctaatg gcccttctag aagaagaggt ggaggacgac tgtgatcgta ttttggaagt
     2881 tgaacaaggg caagtcgtgt ttgaacacgt gaactttagc tatgaagaac acagaccagt
     2941 tttgaaagac attaccattc gggcagaaaa aggagaaaca gttgctctgg ttgggcatac
     3001 tgggtctggt aagtcttcta ttatgaacct cctctatcgt ttctatgatc cacaggaggg
     3061 acgtgtactg attgatgaga aaaacatccg tgattattct cgtgaaagcc tgcgcagtca
     3121 tatggggatt gttttgcaag atccctatct ctttacaggg acgattgcca gcaatgtttc
     3181 catgaatgaa gaggaagcag atagaacaag aatcatgcag gcgctagaaa aagttggtgc
     3241 agggcctatg ctatctcgct tagagaaagg gattgatgaa ccggttgttg agaagggggc
     3301 ggccttttca agtggggaac gtcagttgat tgcctttgcg cggacactct actctgatcc
     3361 taaaattctg atattggacg aggcaacttc tcatattgat acggagacgg aagaaattat
     3421 ccaacatgct atggaagtgg tgaaagaagg tcggacaacc tttatcattg cccaccgact
     3481 ttctaccatt caaaatgcag accagattct tgtcttagat caaggtcgaa ttattgaaca
     3541 tggaaagcat gaagaattgg ttgcacgtgg tggagtatat gctcaaatgc atgagattca
     3601 agcaaaggtg taataagcag ttaaaaattc caagctttgg ctggaatttt ttgctataat
     3661 tatgtgactt ttagtccgtc acgcctcgta aggtgcgtg
//
